LOCUS       DACDYI010000001        77876 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.001, whole genome shotgun sequence.
ACCESSION   DACDYI010000001 DACDYI010000000
VERSION     DACDYI010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 77876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.001"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(194..1363)
                     /locus_tag="IWM52_000001"
     CDS             complement(194..1363)
                     /locus_tag="IWM52_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HAP9972290.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            complement(1434..1984)
                     /locus_tag="IWM52_000002"
                     /pseudo
     CDS             complement(1434..1984)
                     /locus_tag="IWM52_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299296.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
     gene            complement(2187..2666)
                     /gene="rlmH"
                     /locus_tag="IWM52_000003"
     CDS             complement(2187..2666)
                     /gene="rlmH"
                     /locus_tag="IWM52_000003"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAP9972291.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            3094..4383
                     /locus_tag="IWM52_000004"
     CDS             3094..4383
                     /locus_tag="IWM52_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP9972292.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(4422..5027)
                     /locus_tag="IWM52_000005"
     CDS             complement(4422..5027)
                     /locus_tag="IWM52_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAP9972293.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(5232..5696)
                     /locus_tag="IWM52_000006"
     CDS             complement(5232..5696)
                     /locus_tag="IWM52_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9972294.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(5718..6038)
                     /locus_tag="IWM52_000007"
     CDS             complement(5718..6038)
                     /locus_tag="IWM52_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP9972295.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(6182..7261)
                     /gene="pepA"
                     /locus_tag="IWM52_000008"
     CDS             complement(6182..7261)
                     /gene="pepA"
                     /locus_tag="IWM52_000008"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAP9972296.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            7461..7811
                     /locus_tag="IWM52_000009"
     CDS             7461..7811
                     /locus_tag="IWM52_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP9972297.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            8034..8981
                     /locus_tag="IWM52_000010"
     CDS             8034..8981
                     /locus_tag="IWM52_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9972298.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9038..10456)
                     /gene="pepV"
                     /locus_tag="IWM52_000011"
     CDS             complement(9038..10456)
                     /gene="pepV"
                     /locus_tag="IWM52_000011"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAP9972299.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            10605..11450
                     /locus_tag="IWM52_000012"
     CDS             10605..11450
                     /locus_tag="IWM52_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAP9972300.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            11454..11894
                     /locus_tag="IWM52_000013"
     CDS             11454..11894
                     /locus_tag="IWM52_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAP9972301.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            11944..12138
                     /locus_tag="IWM52_000014"
     CDS             11944..12138
                     /locus_tag="IWM52_000014"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972302.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            12274..12960
                     /locus_tag="IWM52_000015"
     CDS             12274..12960
                     /locus_tag="IWM52_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9972303.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIRRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(13368..14057)
                     /locus_tag="IWM52_000016"
     CDS             complement(13368..14057)
                     /locus_tag="IWM52_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972304.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(14032..16161)
                     /locus_tag="IWM52_000017"
     CDS             complement(14032..16161)
                     /locus_tag="IWM52_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAP9972305.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(16209..17546)
                     /locus_tag="IWM52_000018"
     CDS             complement(16209..17546)
                     /locus_tag="IWM52_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAP9972306.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(17798..18157)
                     /gene="rplT"
                     /locus_tag="IWM52_000019"
     CDS             complement(17798..18157)
                     /gene="rplT"
                     /locus_tag="IWM52_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAP9972307.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(18210..18410)
                     /gene="rpmI"
                     /locus_tag="IWM52_000020"
     CDS             complement(18210..18410)
                     /gene="rpmI"
                     /locus_tag="IWM52_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAP9972308.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(18482..19006)
                     /gene="infC"
                     /locus_tag="IWM52_000021"
     CDS             complement(18482..19006)
                     /gene="infC"
                     /locus_tag="IWM52_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAP9972309.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            19326..19958
                     /locus_tag="IWM52_000022"
     CDS             19326..19958
                     /locus_tag="IWM52_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP9972310.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(19960..20865)
                     /locus_tag="IWM52_000023"
     CDS             complement(19960..20865)
                     /locus_tag="IWM52_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP9972311.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(20950..21603)
                     /locus_tag="IWM52_000024"
     CDS             complement(20950..21603)
                     /locus_tag="IWM52_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP9972312.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            21925..22869
                     /gene="mvk"
                     /locus_tag="IWM52_000025"
     CDS             21925..22869
                     /gene="mvk"
                     /locus_tag="IWM52_000025"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAP9972313.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            22862..23839
                     /gene="mvaD"
                     /locus_tag="IWM52_000026"
     CDS             22862..23839
                     /gene="mvaD"
                     /locus_tag="IWM52_000026"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAP9972314.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            23852..24937
                     /locus_tag="IWM52_000027"
     CDS             23852..24937
                     /locus_tag="IWM52_000027"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAP9972315.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            24934..25998
                     /locus_tag="IWM52_000028"
     CDS             24934..25998
                     /locus_tag="IWM52_000028"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAP9972316.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(26247..27050)
                     /locus_tag="IWM52_000029"
     CDS             complement(26247..27050)
                     /locus_tag="IWM52_000029"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAP9972317.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(27070..27912)
                     /gene="kduI"
                     /locus_tag="IWM52_000030"
     CDS             complement(27070..27912)
                     /gene="kduI"
                     /locus_tag="IWM52_000030"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAP9972318.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(27928..28935)
                     /locus_tag="IWM52_000031"
     CDS             complement(27928..28935)
                     /locus_tag="IWM52_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP9972319.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(28942..29586)
                     /locus_tag="IWM52_000032"
     CDS             complement(28942..29586)
                     /locus_tag="IWM52_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP9972320.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(29686..30444)
                     /locus_tag="IWM52_000033"
     CDS             complement(29686..30444)
                     /locus_tag="IWM52_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAP9972321.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(30571..31722)
                     /gene="fucO"
                     /locus_tag="IWM52_000034"
     CDS             complement(30571..31722)
                     /gene="fucO"
                     /locus_tag="IWM52_000034"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAP9972322.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(31804..32115)
                     /gene="rhaM"
                     /locus_tag="IWM52_000035"
     CDS             complement(31804..32115)
                     /gene="rhaM"
                     /locus_tag="IWM52_000035"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAP9972323.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(32117..32962)
                     /gene="rhaD"
                     /locus_tag="IWM52_000036"
     CDS             complement(32117..32962)
                     /gene="rhaD"
                     /locus_tag="IWM52_000036"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAP9972324.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(32976..34232)
                     /gene="rhaA"
                     /locus_tag="IWM52_000037"
     CDS             complement(32976..34232)
                     /gene="rhaA"
                     /locus_tag="IWM52_000037"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAP9972325.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLACLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLEEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(34229..35689)
                     /gene="rhaB"
                     /locus_tag="IWM52_000038"
     CDS             complement(34229..35689)
                     /gene="rhaB"
                     /locus_tag="IWM52_000038"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAP9972326.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            35842..36711
                     /locus_tag="IWM52_000039"
     CDS             35842..36711
                     /locus_tag="IWM52_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9972327.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(36837..37736)
                     /locus_tag="IWM52_000040"
     CDS             complement(36837..37736)
                     /locus_tag="IWM52_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9972328.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(37901..38725)
                     /locus_tag="IWM52_000041"
     CDS             complement(37901..38725)
                     /locus_tag="IWM52_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP9972329.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(38736..40031)
                     /locus_tag="IWM52_000042"
     CDS             complement(38736..40031)
                     /locus_tag="IWM52_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAP9972330.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(40047..40526)
                     /locus_tag="IWM52_000043"
     CDS             complement(40047..40526)
                     /locus_tag="IWM52_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAP9972331.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(40532..41560)
                     /locus_tag="IWM52_000044"
     CDS             complement(40532..41560)
                     /locus_tag="IWM52_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAP9972332.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(41577..42683)
                     /locus_tag="IWM52_000045"
     CDS             complement(41577..42683)
                     /locus_tag="IWM52_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP9972333.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(42912..45509)
                     /gene="adhE"
                     /locus_tag="IWM52_000046"
                     /gene_synonym="adhC"
     CDS             complement(42912..45509)
                     /gene="adhE"
                     /locus_tag="IWM52_000046"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAP9972334.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(45814..46194)
                     /gene="yajC"
                     /locus_tag="IWM52_000047"
     CDS             complement(45814..46194)
                     /gene="yajC"
                     /locus_tag="IWM52_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAP9972335.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(46291..47436)
                     /gene="tgt"
                     /locus_tag="IWM52_000048"
     CDS             complement(46291..47436)
                     /gene="tgt"
                     /locus_tag="IWM52_000048"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAP9972336.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            47654..48352
                     /locus_tag="IWM52_000049"
     CDS             47654..48352
                     /locus_tag="IWM52_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP9972337.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(48520..51159)
                     /locus_tag="IWM52_000050"
     CDS             complement(48520..51159)
                     /locus_tag="IWM52_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9972338.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(51778..52809)
                     /gene="queA"
                     /locus_tag="IWM52_000051"
     CDS             complement(51778..52809)
                     /gene="queA"
                     /locus_tag="IWM52_000051"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAP9972339.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            53002..54177
                     /locus_tag="IWM52_000052"
     CDS             53002..54177
                     /locus_tag="IWM52_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9972340.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            54181..54822
                     /locus_tag="IWM52_000053"
     CDS             54181..54822
                     /locus_tag="IWM52_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9972341.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            54819..55736
                     /locus_tag="IWM52_000054"
     CDS             54819..55736
                     /locus_tag="IWM52_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9972342.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            55733..56404
                     /locus_tag="IWM52_000055"
     CDS             55733..56404
                     /locus_tag="IWM52_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9972343.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(56945..57433)
                     /locus_tag="IWM52_000056"
     CDS             complement(56945..57433)
                     /locus_tag="IWM52_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAP9972344.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(57634..57900)
                     /locus_tag="IWM52_000057"
     CDS             complement(57634..57900)
                     /locus_tag="IWM52_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP9972345.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(57900..58163)
                     /locus_tag="IWM52_000058"
     CDS             complement(57900..58163)
                     /locus_tag="IWM52_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9972346.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(58156..58428)
                     /locus_tag="IWM52_000059"
     CDS             complement(58156..58428)
                     /locus_tag="IWM52_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972347.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            58639..58824
                     /locus_tag="IWM52_000060"
     CDS             58639..58824
                     /locus_tag="IWM52_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9972348.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(58966..60789)
                     /locus_tag="IWM52_000061"
     CDS             complement(58966..60789)
                     /locus_tag="IWM52_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAP9972349.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(61387..64092)
                     /locus_tag="IWM52_000062"
     CDS             complement(61387..64092)
                     /locus_tag="IWM52_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAP9972350.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(64398..65345)
                     /locus_tag="IWM52_000063"
     CDS             complement(64398..65345)
                     /locus_tag="IWM52_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAP9972351.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            65535..65804
                     /gene="rpsN"
                     /locus_tag="IWM52_000064"
     CDS             65535..65804
                     /gene="rpsN"
                     /locus_tag="IWM52_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP9972352.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(65980..66342)
                     /locus_tag="IWM52_000065"
     CDS             complement(65980..66342)
                     /locus_tag="IWM52_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP9972353.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(66353..67474)
                     /locus_tag="IWM52_000066"
     CDS             complement(66353..67474)
                     /locus_tag="IWM52_000066"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAP9972354.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(67490..67840)
                     /locus_tag="IWM52_000067"
     CDS             complement(67490..67840)
                     /locus_tag="IWM52_000067"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAP9972355.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(67970..69481)
                     /locus_tag="IWM52_000068"
     CDS             complement(67970..69481)
                     /locus_tag="IWM52_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP9972356.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(69803..71155)
                     /locus_tag="IWM52_000069"
     CDS             complement(69803..71155)
                     /locus_tag="IWM52_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAP9972357.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(71308..72384)
                     /locus_tag="IWM52_000070"
     CDS             complement(71308..72384)
                     /locus_tag="IWM52_000070"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAP9972358.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            72580..74325
                     /locus_tag="IWM52_000071"
     CDS             72580..74325
                     /locus_tag="IWM52_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAP9972359.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(74605..75042)
                     /locus_tag="IWM52_000072"
     CDS             complement(74605..75042)
                     /locus_tag="IWM52_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAP9972360.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(75026..76399)
                     /locus_tag="IWM52_000073"
     CDS             complement(75026..76399)
                     /locus_tag="IWM52_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972361.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(76721..77209)
                     /locus_tag="IWM52_000074"
     CDS             complement(76721..77209)
                     /locus_tag="IWM52_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9972362.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(77231..77833)
                     /locus_tag="IWM52_000075"
     CDS             complement(77231..77833)
                     /locus_tag="IWM52_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP9972363.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        22397 a        16558 c        14170 g        24751 t
ORIGIN      
        1 aaaaggatag agaaattcaa ttctaaaact atttactaca actaatttat ttatgtatca
       61 atattttttt acattttttt ataaaaaata accacccgaa ggtggctgaa gaataagctt
      121 ttatttgtaa gtttccagaa taggttgtgt caataattat gtgtaaatgg aaaagcccat
      181 tctgttgatc aagttaaaac attgattcta atgtatcatg tatttcttta aaacctttat
      241 gaactcggca tagaaacttt tgattatagt tgttgaattg agatactaga aatctctcca
      301 gagattcttc attaggaaat tgctcttttc ttttggtata tttctttaat tgtttgttaa
      361 aaccttcaat caaattagtt gaataaatag ttcttctgat tgacggaggg aaattataaa
      421 aggttaatat agcaggattc atgagcaatt taaccactcg tggatactgc tttttccatt
      481 tatctatcat aaaccttatt tgattatttg cttcttcttt tgaacttgct tggtaaacta
      541 atttgaaatc gctacagatt tcttgtcgat cactaacacg aaccttatga gcgatatttc
      601 tagatatatg gacacaacat tgttgaaatt gagcatttgg ataaacacta tgaatactat
      661 cgtcaatacc acttaaacca tctgttacga ctaacaaaac ctcttctaaa ccacggtctt
      721 ttagatcttg aagaatctct ttccaaacat aagcagattc agttggagct atagtaaatc
      781 ctagaacctc tttagttcca tccaacctga taccaattac tatatagaca gcttcttttg
      841 atacggtttg tctttttaag ggaatatgag tagcatccat aaaaatgact gagtatttag
      901 cttctaaagt tctttcttta aatgccgaaa catcttcaga cacaatttta ctcatatttg
      961 aaatagtttg tggtttgtaa taatgaccat acattttttc gattagatca gagatttcgg
     1021 acatagtaat tcctttttga actagctgaa taatagtggt ttctaaagag tcattggttc
     1081 ttttataagc aggtaaagtt tgttgagaaa agtctccatt cctatctcta ggtattacta
     1141 aattcaattc accgtactct gttttaaaag agcgcgaata attcccatta cgggaattac
     1201 ctgaattaaa tccagctcta tcatactttt catagtcaag aaataccgtt aattcagctt
     1261 gtaataaagc gttaatagcc aattctaggt gacgacgaaa caaatcatcc aaatcacctt
     1321 tattgattag tatttccata atttctgtag taaaatcgtt catatggaag tcctcttttc
     1381 tgtgaatgtg ttgtggtaac tctattctac agaagggact tccttttttc tatttacaca
     1441 aaatatttta cactctcttc aacgatattc ttgtaagtaa ttatgcccca actaggattt
     1501 gtgggttcat ttccatctgg agttaaaaaa acaaaaattt ttttatagtt agaataattg
     1561 cgttctatat atatttggta tttattaagt tgccctccag actccgaaga cagaatttta
     1621 ttttcgatgc acaccactat tttatttttt tccgatacca ataaaatatc aatattctta
     1681 ttttctctgt aaacaattaa atcttcatag ctattaagta aagcatcaaa ataattgata
     1741 gcagctttgg tatcatcatt agaatctata taccttttca aaaacatttt aaaaaactca
     1801 tcagataaat tgtgggtttc atttggagca aacaaaaaag ctaaaaaatt actatgtctt
     1861 atttcatttt tagatagctt aagtatctga aatatatttg ttttcccagt ccatttagat
     1921 agttttcttc aatatcatcc atatcctcta atagattttt taaacacttt ttttcatcta
     1981 tcatgttttt tctccttgcc attattctaa ctaaaatttt acgtatttgt ttctatgtct
     2041 acattatctt tttaacagta tctatttttc ttttgcaact tacttggcta ccaaagcaaa
     2101 taaaaaagtt atttataaat ttctccctac cactacctat tgctattatt taaaataggg
     2161 ataatttttt ctcttaaaaa atatcattat ttgtggtatg gagcccccgc attgatccgg
     2221 aaggcacggt agatttgctc gacaaggatc agacgcatca attgatgtgg atacgtcatt
     2281 tttccaaaag agatctgagc attgcttctt ttcatgacgg cttcgctgag tcctaaagag
     2341 ccgccgataa caaaaacaag gtggcttttt ccttggatac ctaattggtc gatttgttta
     2401 gcaaaagatt cacttgtagg atttttccct tcaattgcta aggcatagac gtattcttgt
     2461 tctttgattt tcgctaagat ccgttcccct tctttttctt ttacttgtct catttgcgct
     2521 tcgcttaaat tctccggggc tttttcgtca ggaacttcga tcaattcgat ttttgcatag
     2581 gcattcagcc gtttgacata ctcattgata ccttggatca ggtatttttc ttttaatttt
     2641 cctactgaga taattttgat attcatgttt tctctccttt ggtcttcatg ctcactttgt
     2701 ttattataat caattttttc gtctatatgt ttattttggc tatttttgtt aaaaagattt
     2761 ccccaacagt tatgcacaac atccacaggt ttatccacag aaaacagtgt tattttacct
     2821 tatttcatca cattcattag gcaaacatac gttttataag aatttatcca ctttatacac
     2881 aggctgtgga tatgttttat cccttatttt tccaatccta tccgcgtagt tttacacagt
     2941 atctgtggaa aaagaaaatc aaatattttc atctgaaaac aatgattttt cctaaagttt
     3001 ttcataaaaa gttcacagtc tcaaagtaga ctaaagtcaa caaataagac tatactaaca
     3061 gtagataaat gaatgtaatt ggaggaggaa cacatggata gaaaaaatgt gacaccaaag
     3121 atgaaaaaaa gtaaaaataa tagcctctgg cgtaagttag gtctggggct tgttggaggg
     3181 atcgttggag gactactgac agctggcatt ttttatgcag cgatgggcac tggcaacaat
     3241 agttctactg ctacaagcgg caatcagaat tctgccggtg aaacagttgt ggaaaatgtc
     3301 aaagtcaatg tagattctga tatcaccaaa gcggtagata aagttcaaga cgctgtcgtt
     3361 tctgttatca acttacaaag tcaaaaccaa tcaagcggtt ttggcggact attcgggcag
     3421 caagaagaaa gtagcagctc agatgatagt aatttggaag cttatagtga aggaagcggc
     3481 gtgatctaca aaaaagacgg gaatactgct tatgtagtca cgaataatca cgtagtagat
     3541 ggccagcaag gtcttgaagt cttgatgaaa gacggtacga aggtaaaagc tgaattagtt
     3601 ggaacagatg cttattcaga tttagcggtg ttgaagatca attcagacaa agtcgaaaca
     3661 gttgcctctt ttggtgactc aagtgctctg aaggttggtg agcctgcgat tgcgattggt
     3721 tccccactag gatctgaata tgcaaattct gtcacttcag gaatcatttc ttccttgaac
     3781 cgacaagtga caagtacgaa tgaatcgaac gaaacagtca atatcaatgc gatccaaaca
     3841 gatgcagcaa tcaaccctgg aaattctggt ggtcctttag ttaatatcga aggacaagtt
     3901 atcggtatca actcaagtaa gatcgcaagt acttctgaat ctacttctaa tgtaagcgta
     3961 gaaggaatgg gatttgctat tccaagtaac gatgtagtga atatcatcaa tcaactggaa
     4021 aaagacggaa aagtgacacg tccagctcta ggtatcacaa tggtcgactt atctgccgtt
     4081 tctactcagc aacaagaaca aatcttgaag attccttcat ctgtaactaa cggtgtcatc
     4141 gtgacaaatg tccaaacagc tacaccggct gaaaaagctg gattgaaaca atacgacgtc
     4201 attactaaga tcgatgacaa agaagtttct tctggtgtag aacttcaatc tgctttgtat
     4261 aagaaaaaag tgggagattc tgtcaaaatc acttactatg aaggctcgaa agaaaaaaca
     4321 actactgttg aattgacgat cgatcaatcg gcattaaaac aaaatcaaaa tagtgaaaat
     4381 taataaaaaa gaggttgtga caaagacttg tcacaacctc tttattttgg aaatgcttct
     4441 ccgactacgg catcctcagg caatactaag attccttttt ttgctggagc atttggcaaa
     4501 tgcaattcac gagcggaaca gatcattcca tagctatcta cccctcgcaa agttcctggc
     4561 cagatcatca ttccatctgg catcatagct cctggttttg caacgacaac tttttgtcca
     4621 gcttcgatat tgggtgcacc gcaaacgatt tgcaatactg taccattgtc tacttctgtc
     4681 tcagtaatcg ataaatggtc agaatcagga tgtgggacgc atgtttttac gtacccgaca
     4741 acgatcttcg gttcatgatc agcttcaagc tgttcttcga atcctgcttc tttgatcaat
     4801 cgattcaatt tcgctaattc ctcatctgtt aagcttactt ggccgttccc ttcgattgtt
     4861 agatgatcgg agatatggaa aaagttccaa gcaacaactt gaccttgttc atctttgatc
     4921 cgagcaacat tccatttgcg ttcaacagtg ttttctgttc ctttatcgtc ggctataata
     4981 accatcaagg tgtctccgac gtgtgctttg ttataagcaa aaatcatgtt ctttcctcca
     5041 ttatcctttg ctgagattta tcttaacaaa aaattgcatt cactgcatga aatttttttc
     5101 atgctttatt tccgaagaag cctcttctgg aacaccattt attcgtaaca agggactgtg
     5161 acaagacttt tgtcacagtc cctttactgt tttagttaat tgattaaaaa ggtcgtacaa
     5221 gttccacttg cttaattact tggctgttct tcttcgtcag aaacgatcaa gacatcacaa
     5281 ggtgaatgac gtatcacgta gctggctaca cttcctgtca taaagcgttc gactgcattt
     5341 agacctgact gcccaaccat gattagatcg atctcatatt tttttggcaa tttgactgcc
     5401 atggattcac gaacagaacc atatgcaata attccttcta tatttgtgta atcaacagat
     5461 ttcccatatt ctttacattc ttcgatcatt tccttggctg tttcagtttg ctgatcgact
     5521 agattttgat caataggtgc atagcccata aagttataaa attgctgatc gattacgttt
     5581 gctacatata ctttcccatt attacggcga gccacttcta ccgctttttt gaaagcagca
     5641 agtgcttgtt cactaccgtc gatacctact aaaatattct tatattcttg attcatcttg
     5701 acccctccta acagatgtca gccttttgtt acactttcta ggaacgattc gatttcctgt
     5761 tttgttttac gatctttatt cactagacga cctaattctt ttccgtcttg gatcacgaca
     5821 aaacttggga taccaaaaat gttccattca gcggctacat ctatgtactt gtcacggtct
     5881 acttcaataa attgccaatc agaaaagtca ttttcaattt caggcatttg cggtttgata
     5941 aaacggcagt ctccgcacca gccggcagta aagaaaaata cactctttcc agtttctacg
     6001 tagcctgcta attcttctaa actatttggg ataatcattc tttgtcctct actttctgtt
     6061 tatgtatttt tcaattaaat tatacacctt tctttcgaac taacaaacat tgatcctctt
     6121 tttataaaaa aaatctggca aaaagcaatc ttttgccaga ttttatagtt tcactatcgc
     6181 attattttcc gtatacgatc gtatttactg ttgatttatc caaacctttc agtgtttgaa
     6241 tcaacatttc tcgtgcagct tcaaaatcgc gaatactgaa catcgtttga tgcgtatgga
     6301 tataacgtcc cactacgcca atcactgtac ttgggattcc ttcgttttgt gtatgagcag
     6361 cacctgcatc tgtaccgcct tttgaaacaa agtattgata agggatattg tttgtttcag
     6421 cgatatccag caagtactcg cgcaaacgag gaagcatgat caaacctgga tcttggatac
     6481 gcatcaatgt accttctcct aaatgtccga atgtgccatt tttcgtcact gtatcatctg
     6541 ctgcagagca gtctactgca aagaacagat ctggcttgaa tttattgaca gaagctttag
     6601 aaccacgcaa gcctacttct tcctggacat tcgcgcctgc aatcaatgta tgacctaatg
     6661 tttcattttg caacgcttct aaagcttcta ataccattgt acagccatag cggttgtccc
     6721 atgatttact gataatattc tttccgttgg ctgttttgat cgtttcagcg taaggaacga
     6781 ttgtgtctcc tggaaggaca ccgtaactca ttgcctcttc tcttgattca aacccagcat
     6841 cgaacaaaat atcggttact tctactgatt tttgtccgct tgttcctcgc aataaatgtg
     6901 ggggaacgga agaagaaata catggataat tcttaccagt agaggtttta agtgtaaaac
     6961 gctgagcaga aacaacgtaa ggattccagc cgcctagagg tacgacttta aaaagcccgt
     7021 tttcttggat ttgtgtcagc atgaatccga cttcatccat atgtgctgcg accattacac
     7081 ggggtgcatc cgcttcttca tgatggcgca gaccaaaaat gccgcccaaa ccatctaact
     7141 gtaactcatc taccagcggt tccatatgac tttccatata agcgcgaaca tcttgttcaa
     7201 aaccgcttgt tccttgtaat tctgttaact ctttgattcg ttgaaatgtt ttttcttcca
     7261 tatgctattc gtctaaattt cagactaaac tcctctcaga cttatattga tctgcggata
     7321 aaatatgatt ttttctgcag accgatcgta tttattatag cttatttttt ctggtagcgg
     7381 ttatttattt caaagaaaat atgagagtgt ggtaaaataa gcacaaggag taacaagtga
     7441 gaagaaggag gtatgtccac atgtatgaag aaagcgaact aagctatttt aaaggcgggt
     7501 tagcagttgg aatcggtcta ggattattga gcggggtagc ttcaacgatt tggtatcatc
     7561 gcaaaaaagt attgtcagca gaccaagtac ttgaaaatat caagtcatct tttctaaaag
     7621 aaggtgcaat cgaaggttct tggatctctt ttgaaaagga acctattagg aaattcgccg
     7681 ttcggtcgag tgcttatcgg ggaggtcttt ctcgaataga agatggcgaa actgtttatt
     7741 atgaattttt agcagatgct catacaggaa ccgtattaga aatttcaaga aaaaaaggtg
     7801 caacagatta atacgtcaaa aaagtgagag aacgaatacg cacccactat tcaaaagaga
     7861 ggtatcatgt ccttcttttg tagtgggtgg ttttttagat agagctttcc aaacatatat
     7921 tccagtattt tctttttaaa taaatgatat ttagcgggaa aaactagcat ttcctttgat
     7981 tatgaggtat ttttaattta gcttttgttt tctatagtta aggagagtga aaaatggaac
     8041 aacaagaaaa aattgctgtt ctgattcctt gttacaatga ggaagcaaca atttcaacag
     8101 tcattgcaga ctttaaacga gaattaccag aagcagatat ttatgtctat gataataatt
     8161 cgaccgatcg aacatacgaa ttggccgttc aaggtggagc aatcgtaaaa aaagaacctc
     8221 gtcaaggaaa aggaaatgtg atccgccaaa tgttctttga tatcgatgcc gactattatt
     8281 taatggtaga tggcgatgat acttatcctg cagaagctgt acatggtttg ctcgagaaat
     8341 tacgttctgg tgaagcagat atggtcattg gtgatcgcct atcaaatgga acctactttg
     8401 atgagaataa gagacctttc cacgattttg gaaataacct tgttagaaat acaattacta
     8461 gaatgtataa aacaaaaatt cttgatgtca tgaccggcta tcgaggattc aatcgtatct
     8521 ttgtcaaaag ttttcctatt atgagttctg gttttcaaat cgaaacggaa ttgacgatcc
     8581 atgctttaga taaaaaattc aagttcgttg aaatcccaat cgattatcgt gatcgtcctg
     8641 aaggcagcga atcaaaattg aatacattct ctgacggatt caaagtgatc atgatgatta
     8701 ttaaaatgtt taaagattat aagcctttgc ttttctttgg gatttggacc atcttcttct
     8761 ttttatttgg attggtcacg gggattcctg tgattcgcga atatatgctg acaagcttta
     8821 ttacacgcat cccttctgcg attttaagta ctggattgat gactttagca cttctttcat
     8881 tagtaacagg gttgattttg gatacagtcg tcacgaacgc taagaaagaa tatgaattga
     8941 atctatatca tgtttatcat gaataccaca gtagaaagta aaaaaaggat caagactgca
     9001 aaaataattt gcaattcttg atcctctttt taattaatta tttcgccaaa cgatagatgg
     9061 catctgcata gattgctgtt gctctgaaca agtcatcaag actcataaat tcgtttgctt
     9121 gatgcattgt atcagtatat cctgggaaca tcgctccata agcaacgcca cgtttcaaca
     9181 aacgtccgta agttccgcca ccgatgattt gttcgtagcc tttttctcca gtgtggtctt
     9241 catagacttg caataatgta gccaccaatg gatcatccat tggtacgtaa tgtggttcca
     9301 ttacgcgagc acctcgagta actgttgcgc cttcttgccc cactgtttgt tcgatgtcac
     9361 tttgcaatcc gtctactgtt acaccttttg gaaaacggaa attcatattg atgaagttcg
     9421 cttcctctgt accattttct tcaaaagcaa atagtccagc attcattgtc aatttgccca
     9481 ttttttcatc ttcataagcg actcctagtt tttctccata aaaatcttca tggacataag
     9541 cagctgctac atggataaat tgtttagcag atcctaggaa ttcatagtta tctaagaaca
     9601 atgccaagaa agaacctgca ttgataccag attgtgggct tgcaccatgt gcgcctttac
     9661 caactaattc gatttttaca tacgtattgt ccgcttcaat cgttccgctt actggatttg
     9721 cttcgatgaa ttggtaaaat gcttcttcca tcgcgatagc tgcatctgca cttggtactt
     9781 gaagtgctgc agttgctgtt cctggcacca tattttcgcg aagtccggaa acaaaagatt
     9841 tcaagacata atctcctgca ttgtcgcctt tgaagcgtaa agctaaagaa acatttcctt
     9901 tttcaccatt gatgattggg aattcagcat ctggtgagaa accaaaatct ggttcttctt
     9961 catgttcgaa ataataagcc atatcgcccc agccgctttc ttcatcgctg ccgactacga
    10021 agcgaacttt ttttgatagt ttcaagtcaa gttctttaat gatcttcatc gcatagtaag
    10081 ctgccatact tggacctttg tcatcgcttg atccgcgtgc aaagatcttt ccatccttga
    10141 ttactggttc ataaggatct gtgtcccagc catctcctgc aggaacgaca tccatgtgtc
    10201 caaatatccc tagcgtttcg tctccttcgc caagatcaat atgccctgca tagttatcga
    10261 cattttttac tttaaatcca tcacgttcgc cataagctaa catatgtttc aatgcgtcgc
    10321 gaggtccagg tccaaatggt gcatctggtg ttacttttga gtcatcgcgc tcgctgttga
    10381 cacgtaaaag atttttcaag tcttcaagca agtcttcttt acgtgcttct acttcttttt
    10441 gccaatcaat tgtcataaag ttaatcctcc acttatttaa gtcttcgtgt tcatgatacc
    10501 acaaattaca gaaaaaaata gttcaaaaaa tctgacaatt ccttttctta tctgtttttc
    10561 tctaaaaaaa atatacgcta taatgagtaa aaggaggttt ttagatgctg ataaacgaac
    10621 aactagtgaa aagagtcatc acacctcgtc caaaaatttc acataaagga acatttggac
    10681 gtatcgtttt gattggcggc aatgaacaat atggcggagc aatcattatg agtgctttag
    10741 ctgccgtaca cagcggagcg ggtctcacga ctgtagtaac tgatgaaaaa aatcggagtg
    10801 ctcttcacag tcatctgcca gaagcaatga ttgtagtttg gacggatact gaccagtttc
    10861 tttccgtaat aaagcaagca gatgtactct tgattggtcc cggattgggt acttcttcta
    10921 agagtttgga acgtttcaaa caagtactag aaaaccaatc aaaagaacaa tggctggtga
    10981 ttgatggctc tgctttaagt ttacttgctg ctaatcatct tttgctaccc tatcccgaac
    11041 aaactgtact tacacctcat caaatggagt ggcaacggtt gagttctcta ccgatacaag
    11101 aacaacggga taaagaaaac aaaaagaagc aacaggaaat cggtgctgtg ctcgttttga
    11161 aaagtcatga gacaaaaatt tatggtcacc agcacttcta caccaatcct ttaggttctg
    11221 ctgctatggc aacgggcggc atgggtgaca ctttagctgg gatgatcaca ggatttttag
    11281 cccagtttcc tcaaaatgac caaacgattg cagcagctat ttatcttcat agtttcattg
    11341 gagaccagct ggcaaaagaa caatatattg ttttgccgac acaaatcagc gaagcacttc
    11401 cgaaatggat gaattatttt tgtaaaaaat caatggaaaa cgaggcatga gtcatggaga
    11461 aatttacagg ttatgtgcga gaaccttttt attatgaaac agatcaaatg gggattattc
    11521 atcacagcaa ttatatccgc tggtttgaag aggctcgggt agccttattg gtacatctag
    11581 ggtttcccta taaaaaaatc gaagaatctg ggatcatcat ccccgttctg gaagtaaatt
    11641 gcagctataa agaaatgatc cattacggag aaaaagtgag catccatcca actatccaaa
    11701 agtatacggg gacacggttg gattttagct acgaaattgt gggaactgaa gacaaaaaat
    11761 taaaaactac tggcagtagt aaacactgtt ttttactttc tgattcgcaa cgattagtga
    11821 agctgagcaa agtcaatccg gagttagatc agttattcca agcgtacgct tctttggatg
    11881 atgtaaattc ttaataaaga ccgtctaagc atttttcttg tattgtgtcg agtagttcgt
    11941 ataatgaaaa gaaaaaagca ggtgatcccc atgtatgtcg ttaaagtaat gcacggttac
    12001 atcgataaaa ctggctgtcg cactcgggaa aaaaacttag acaatctgct cattttcaaa
    12061 gacaaaaaag aatccgaagc ttttgctaaa agaatcggcg gtcgtgtcaa accgatccaa
    12121 gaggtacgac cagattgatt caatattttt cttttcgagg ctctaatagg catgcctatc
    12181 agggcttttt ttgcagactg tgtttttatg cccacacgat ttgtaagcga tttatgttat
    12241 aatagattat atctgcatag aaaggaaggt taaatggtta tcaataattt gcaacagcaa
    12301 gcttatgaat ctatacgtaa acaaatcatt tattccgagt tagcacctgg aagtaaaatc
    12361 tccgataaag tgttagaaga acaactagct atcaggcgta caccgattcg agaagcactg
    12421 atccaactgc gcaatcaaga gcttgtaaaa accattccac agtccggcac ttatgtttca
    12481 aaaatcgata tccgatctgc ttcattagcc cgttatacgc gggaaaagtt ggaaaaaccg
    12541 atcctgcaag aatgcagtgc gaaaatgacc aagcaagatc aagctaaact agaaaaaata
    12601 ctagaacaaa ccgatcaggc agtaatcgct caaaataaaa aagctttttt tgatttggat
    12661 actgattttc accgaacttg ttatgaaatt gctggaaaac gagaaatttg ggattggcta
    12721 gaaagttata gtacgcactt gaaccgtttc agatggttac gtttgacgat ttcagaatta
    12781 gactggggtc gagtattaga tgaacatcag acaatgcttc aatcaatgat tgatcataac
    12841 tttgatgaag tcggatttct ttgcacgatg catttacata tgattatcga ggagcaagaa
    12901 tacgtgattc ataattaccc cgattatttt gaagatattc aggacagacc catcaaataa
    12961 gaggttttgg aatcagcgtt ttgacctgag tattagagaa agaaacggca aaatagttcc
    13021 tcatattttg ccgttttttt tgagctaata ccgagggtca gctgattgca caccgtttat
    13081 tgagaggttg tgaaaaagct gccctgcatc aagtaataag aacagccaag taaaaatagc
    13141 ttcttatgtt tttaacttgg ctggacttat taccgcagat gcaagctttt gaacaccgtt
    13201 ttccaagagg tcgtgagcct gccgttcatc tccgagtcac aaggaacaat ttcaaaaaac
    13261 agcttctcat gtttgttgaa tattttgact tgtgaccgag gagttgacag gagaacaccg
    13321 tttattcgga aaaaaaggac tgtgacacga cttttgtcac agtctcttta tttgtaatct
    13381 aaatcatact ccgtacatct tttttctatt ttttttattt cttctaagat cccttggcta
    13441 cgaatttcat tatatggcca aagtaagact gcctctaata cataaggcga aaaatcgctt
    13501 tgattcatct cttttgaaaa actcatcgct gctatctcgc tcaatgcacg aatgtttgat
    13561 tgatgtgtat aaaaaggcaa ccgccacagc acaatttttt cgctttgcgt ataaattccc
    13621 ttaacagaag cttcgatgtg gtgaaatagt tcatgtccaa taatcaaagc ttgcgctgtt
    13681 tctttagaga tcgaagctag tcctgaacat tttaattttt ctaacggctc cgtcatcaat
    13741 cgaatccctt catcttctgc ataagttgca aaagtgattc gttcgtttat ttgctctgat
    13801 tcacaggaaa cgattggaac agccatttgt tcacaaattt ctcctacatc ggcaaaaggg
    13861 tacttttttt tgatgctaca tgccgtttcg ataccacaat cgattgattg ttgtataaga
    13921 attttctgct tggacaaagg aatctttttc caaaataaat ctcgccgaaa ctggtagaat
    13981 ccccaaattt cttcggggat ctgttgtaat tcgtttagta actcttctaa cttatgtttg
    14041 tttgatattt ccggcaacga cgatcacttc ttcctccggt tccatagtag atacttccag
    14101 atccatcaaa agctgtagtt gttccagatc cgtcgctgta aaatcagaga ctcttgctcc
    14161 taagcagaga taaaattctg gtcgggcaat caattcagtt tgataaactg ccatatcttc
    14221 ccaaagttgt tcgacagctg tcatataatt ggctgcagtt gttttcatac atgaagcatg
    14281 tccacgttgg accttgataa atcctttttg atcgatgatc cgaagacttc cagcattttt
    14341 tgaacgattc tgttcgccgc atacataaaa gaaaggtgta ttttccaata agcaaacttc
    14401 atttttattt aatcgcatat cctctgcagc aagctctagt gcttcttctg ttgtgatctc
    14461 cttggttaga tcggttgctt tcacttccgt agaaccagta gcgatagccg ttacttttga
    14521 agtctgtgga tcgatttcta cgtggacttc gatcgattct ggtgtagcgc ctgattcaat
    14581 cgctttattc atcgcttcat ttttcaacga tcgtatatct tctttacttg gcgaagggat
    14641 gatacgttct acaacatctc ggaccattgc aagtgctaca ccaatagaag aaatcacttc
    14701 ggcgttttca ggaatcgaat atttcacacc cattttatta ctaaagtaag taatcaatga
    14761 ggcagcgccg ccccctacac ctacaagaga aatctgatct ttctccaagt gatacttgtc
    14821 tgccaattct aaaatcaccg gttcaatctt agcataggat ttttccatga tctgttctgc
    14881 gatgtcttca accgtcgtat gacaataatc agctaatgct tggatggctt ttcgtgcaga
    14941 tgggacattt ccataagaaa aatgttcttc ttgcactaat ccaaggacat tggctgcaca
    15001 tgtatttgta atagtcactt cttctccgtc ttccatcact accttgacgt aatcagcagg
    15061 gtctccttct ttaggagaga aaaattcaac cttaggacct ttgatttttt ctgtctccgt
    15121 aaatactgca taatctagac cggcaatatg tgcagaacgt ggaccgacat cgatgattcc
    15181 tgactgatta gcccgtacca tcgaaccgcc tgccacacct agaacccgaa catctaatga
    15241 agagatatat gttgggtggc cacctacaat tgaataatcg attgctggac gtccattttt
    15301 aatgaccccg atattggttg ttgttccgcc tacttcaaaa taaacaccat tagacgctct
    15361 caagtacatc aaagagccca tgactgatgc tgctggaccg gaaagcatgg ttaaaacagg
    15421 tcgttttttc atttcattga tttccattac tccgccgtct ccgcgcataa tcatcaaaga
    15481 aacattgacc cctgcttctc gtacggaatc ttctgtagag gttgccgtat ctagcatttt
    15541 tggtaaaata gatgcattga ttgctgctgt ccgagttctt cttgtcaatc catataattt
    15601 tgtgatgtca gaagccatcg ttgtagggat cgattttacc gatgctgctt cataaacaac
    15661 tcgttctggt tccccattat ctactccaaa tgccatagaa gaaaccaaga cctccgctct
    15721 ttctctttct aaagaagaaa tcgtttctga aacacggtct acatttaagt gtttgacagg
    15781 taagaatgca ttgacaatct ctattttttt cttattccct aaatcgatat cgttcaatct
    15841 agtttgtcgt tttgctaaaa aaccttctaa tccacctttt gccatcccga ttacgccgac
    15901 ttttgctacg tctccttcaa tcagtgcatt agtcgcttgt gtggtagaat gtgcaacaaa
    15961 cacaacatct tctggactga tattattttc cagcaaacaa ttttgaaaag actgaacgac
    16021 acctgcggct actccacgga catcatcgtg ggttgttttc actgaagatt ttccgatgat
    16081 ttcgtgcgtc gcattatcga ttgcgactgc tttcgtatgt gtaccgccaa catcaattcc
    16141 cattcgcaca aaacgtttca ttttcttttc ctccattcat ctaatcgttt ttatcgacgt
    16201 tttgtttact aggcgttccc gtacaataag aacgtgatca ggcataagat cgctccgatg
    16261 atccaaccag ttgggatgga tttcttcata tattctcgag aagttacttt tgtataacct
    16321 aatccccaag caacccaaga ttgtgtaata tctaaatgct gaggagcaat cgttgtgatt
    16381 gcaaataacg ggaacaaaaa ggctggcgac atcgttggat taacagctaa tacgacagct
    16441 aaaatagctg agcctgaacc gactagattc attggaccgc ggaaaaatcc tagtggtgtc
    16501 aatatagcaa atacgattgc caagactaat ggtgattgcg gtatccaatc tccaaaaata
    16561 gctgaaaaat aaggagaagc atacgcagct gaattattga acatcgctaa ggtcatcaag
    16621 aaaccaacca ttggcgcaac atctactgcg ccatctgtaa ataatttcgc aaactgacga
    16681 cagatttctt tataacctcc gcgcaatttt ccagtaaaca gcaatgccca gatacctgct
    16741 agaataaaac ctaagatgat tggaagatct aaaagaacga cccctaaaac tggtaataat
    16801 acggctaacc atgagatcat tggtgcgtcg ccaccttcag ctggtacgtt tgctgccatt
    16861 gcatagcgtt tctttttgtt cattgatata ttagcgaccg ttagtaccac aactaagctt
    16921 acaatcatcc caatcatacc aatttggaaa tagtcattat aagtatagct ttcagccgct
    16981 ggattgaaac cagcataaat cgtttgatat tgtttgaaat tgacgatatt agcaaagatt
    17041 ccagccataa tcgagcccat aaatgagaac agtgcaaccg gtactgaaat acctaatgac
    17101 atcaagattg gcaacacaat aactgcaata gaaatcactg gtccgattcc tgtcatcgac
    17161 atgaatataa cagcggttac aatacatagt aagctcatcg tgattcttgg cttgtcccct
    17221 ccaagttcaa ctacctttcg aatcagggta gctgcgattc cgctatctac tagaacacgc
    17281 ccgaaaaagg caccaaagaa gacatttacc aagatcgatt tggcgtactc agctggcccc
    17341 gtcgtataga tatgcgtcaa cacatcaatt actccttgcc cttccatcgc cgggtttggt
    17401 gaaaacgtgt ttccgactag cgctaatccc atccatacag tagccatgat ggcaaagccg
    17461 accatcaagt tgtagccttt tacacagtac cagaccatga aaaagaatga gataactaac
    17521 aaaataccaa taataatatc actcatgtta ttcctccgtc cattttgttt ttctgataag
    17581 caaactaata tatcagtcaa actcattcta acttatgtag ttagcgcttt cattaaaggg
    17641 tgaccataaa tgttcagttt tcaattaaaa aaccttaatt atgctatttt agacttatac
    17701 gttattgtcc tgtttcattt gatggaggat tgtaaataaa aaaagaggaa agaacataac
    17761 ttttcgttat cttctttcct ctttataatt gtttagctta tttagccaaa gcttcttttg
    17821 cttggtcagc caatgcagta aatgctgctg catcgttaac agccaagtca gctaacattt
    17881 tacggttgat gtcgatttca gcaactttca aaccgtgcat taattttgag tagctcaagc
    17941 cattcatacg agctgctgcg ttgatacgag caatccacaa tttacggaag tcgcgtttct
    18001 tttgacgacg atcgcgatat gcgtagttgt atgaattcat tacttgttct tttgctgttt
    18061 taaataaagt gtgttttgaa ccgtagtaac cttttgctaa ttttagcact cttttacgac
    18121 gtttacgagt tactgttcca cctttaacac gtgccatgta taattcctcc taataaaaat
    18181 aaatcttcag attctgttat ttgtacttct tatttcattt ttgctagttg ttggcgaata
    18241 cgtttgaagt cgccactaga aaccatgctt gctttacgca attgacggcg ttgttttttt
    18301 gttttgccgt ggaaacggtg acttgtaaac gcgcggaagc gtttcaatcc gcctttacca
    18361 gtacgtttga aacgttttgc tgatccgcgg tgtgtttttt gttttggcat gactatattt
    18421 cctcctcaaa atctttcgtt atccgactat cggctgtcca acagccagtt agtcattcaa
    18481 attacttact gtcgtttttc ggtgccagcg tcaagaacat gctgcgtccg tccattttcg
    18541 ctttttgttc cactgttgca atatccgcag tttcttcagc taagcgattc aagacttttt
    18601 gaccaatttc tttatgggta atggcacggc ctttgaaacg gatagaagct ttcactttat
    18661 cgcccttttc caagaactta cgtgcattac gaagttttgt attgaagtca ttcaagtcga
    18721 tcgtaggact taagcgaact tcttttacat tgatcacttt ttgtttttta cgcgcttcgc
    18781 ggtctttctt ttgttgttcg aaacggtatt taccgtagtc catgattcgc gcaactggtg
    18841 gtttcgcacc aggggcaact aacacaagat ccaagtttgc ttgttctgca atattcaatg
    18901 cttctgcttt tgtcttaact cctaattgct caccatctga accgatcaat cgtaactctc
    18961 gtgcacgaat gccgtcgtta accatcatat cctttgctat ggtcattcac ctccaaatat
    19021 tatgagagaa agaaaagctt accgaataaa cggcaataaa aaaacgagtt ctcttacaga
    19081 acccgcgcgt acttcaccta atagaaccag tgaaaactat ctagcccagt gacattatca
    19141 actaaggcga gaagcgggtg cttctgcttt tatttctcaa ccatcatagt atagcaaaaa
    19201 ctaatctttt tgtcaatgtt tttcatacag tttgtcttcc ttttctgaaa atgattagat
    19261 tagcagactt tctagcagtg attcccgctt ctttgctata ctaaagaaaa acgaggtgaa
    19321 cccacatgaa aaaaatcgaa cctattccac cttctcttcc tcttcttaca aagggagagt
    19381 tttatttaga agatgaggag atcttctctg gtcttctgat caacgggggt gaccaaagct
    19441 atctcgatgc caaaaatatg gtattaagag agagtcatat ccaaaaactt acgatgcaaa
    19501 aaacagtttt acgtcatttt gaatgcagta atgtcatttt tgaaaaatgt gatttttcca
    19561 atctggagtg gattggtggc agtttccacc aagtcgtgtt tcatcaatgt aaattaacag
    19621 gtacgaattt tgccgaaagt tatcttcgag actgtacttt cactgattgt attgcttcga
    19681 tggcttcctt tagtagtaca aacttgaaag ctgtctcctt cgatcattgt caattagaag
    19741 acagcgaatt ttatgaggtc acttggaaaa accttttttt gtctgacaat cagttgaccg
    19801 gttcgaactg gtttcgaact tctctgaagg gattagactt tacgactaat ttcttcacag
    19861 caatcgcttt atctcaagat tctttggcgg gactaatcgt caaccaagag caagcacttg
    19921 tgatcgccca agcccttggt ttgcagatca aagactagat caacgaatcc gccgccacag
    19981 tgcaataagc atccaaatag ataagaccaa actgatgatc aaggcgattc cccatccgaa
    20041 aacggaatgg gtaaagggta actggagatt cattccgaag aatcctgtca caatagttgg
    20101 aacggtcaag atcagcgacc agaccgtcaa aaatttcatc gtgtcattca agttgttatt
    20161 cagcaaatta ttataagtgc cagagagctg gtctaaaatc tgcgaagcta agttcgtcat
    20221 ttcaactgct tgtcgtgctt cgatcagcgc atcgtccaac tcttcttttt ccttttctga
    20281 caacttgcga taaatagcca atgccttgat ttgttctaaa agtactacat tttgttttgt
    20341 tcctgtaact aagtaaacca aaccgatctc taaatctgaa agttgcagca gattcttgtt
    20401 cgttgttttt tctcttaatt tcagattaag ccgctgacgt tcactattca cttcctcgat
    20461 taaaggaaaa aaggaatcag aaatcaaaaa caatgtacga aacaataaac tgtatacact
    20521 catgtctggc attcgttcca aaagcgatcg aattagtcga atcacgtact cggtattcgg
    20581 cgtggtaaat gtaaacaaac cgtcttcttt taaaataaaa gcgataggac tagtttcata
    20641 atgattatcg actttttgct ggtgcggaac atttgataca agcagtaacg cttcatcaaa
    20701 agaatcgtac tctacgcgcg cccgttcatt tttatctaga gaataagtca gcatctcgtc
    20761 actaatgcca tattcttctt ttaagttttt aacagctagc acatcgtttt gctgtacatt
    20821 gatccaaaaa ttacggtctt gattaaaata gtgtttttcc cgcatactct atcctcctga
    20881 catttgtttc cagtataaag aaaaaagtcg ctcaaataaa ggtttaccct ttatttgagc
    20941 aactgtaaat caaatggcta ctttggtttg gcgaatctct gctaattttt cttcgatcat
    21001 tttcaagact tcttcttttg cttgaggatc ttccacaaaa ttatagcggt ctccatcgat
    21061 ttggattttt ggactttctt tgtattcttc ataccattga tcgtaacgtc tgttcaattc
    21121 tttgtaataa tcgtatagac ttggatcaaa ggacaattgt tcatacgaac gaccacgttt
    21181 ttcgatacgc tccaacatcg tatcaaaaga tacacgaata tgaactaata aatccggatg
    21241 ttttttatgg gcagcatagg gaagttcttc catcatgttt tccagcagtt catcatacac
    21301 acgaacttct gtttctgttg ctcttcctag atctgcattt agatggaaaa gcagtgaatc
    21361 ttcataaatc gaacggtcca atacattatt gtcatctttc attgcttgtt ttatgcttgc
    21421 aaaacgttta ttcaaaaaat aaatttgcag caaaaaagca tactgttcag ggtttgcata
    21481 aaataagggc aagacttcat tatcatcaat cgattcataa aatgcctgac tatcaaagtg
    21541 ttcagccatc atttctgtta aacttgattt tccggcacca attgtacctg ctaaaacgat
    21601 cacgctcatt tacctccagc tcacttcatc actaaatatt gtgtttaact ccaacctttt
    21661 taaatatacc cccaatatct agtagtgtca attgtatttt tttctagtca aagaagaaaa
    21721 aaagtcaaaa aattcttaag aaaaaagtgt tttttcttta gaaaacgtat tcttagtttt
    21781 acttctgaga tggttttagt aaaataaagg aggaatgttt tttgagaccc ttagtggaca
    21841 aaaaaattcg tgaagcagcg caactcgttt tgggcgcttt attttttgct taaaaatcac
    21901 ttttacgtga gaaggagtta gattatggca aactatggcc aaggagaatc aagcggaaag
    21961 atcatattga tgggcgagca cgcggttgtt tatggagaac cagcgattgc ctttcctttc
    22021 tatgcaacaa aagtcaccgc attccttgaa gagctggatg caatggacga tcaactggtt
    22081 tcttcctact attcaggaaa tttagccgaa gctcctcatg cattaaaaaa tatcaaaaaa
    22141 ttattcattc acttaaaaaa acagcatgac atccaaaaaa acttgcaact gaccattgaa
    22201 agcacgattc ctgctgaacg tggaatggga tcaagcgctg cagtcgccac agcagtcact
    22261 cgtgcttttt atgattactt agcatttcct ttgtctcgtg aaatactatt agaaaatgtc
    22321 cagctttcgg aaaaaatcgc ccacggtaat cctagtggaa tcgatgcagc cgctactagc
    22381 agcttgcagc cgatttattt tacaaaaggg catcctttcg actacttttc tttgaacatc
    22441 gatgcttttt tgattgtcgc tgatacagga atcaaaggac aaacaagaga agccgtcaaa
    22501 gatgtcgctc acctctttga aacccagcct catgaaactg gacaaatgat ccaaaaatta
    22561 ggatacttga cgaagcaagc aaaacaagca atcattgaaa attcaccaga aacgttggca
    22621 cagacaatgg atgaatcaca atcacttctg gaaaagctga caatcagcaa tgattttctt
    22681 aatctattga tccaaacagc aaaagatacc ggggcattgg gtgccaaatt aactggcggt
    22741 ggacgcggtg gctgtatgat tgcattagca caaacaaaaa caaaagccca agaaatcagt
    22801 caagcacttg aagatgcggg tgctgctgaa acttggatac aaggattagg agtacatacc
    22861 tatgtttaaa ggcaaagcac gcgcatatac caacattgct ctaatcaaat attggggtaa
    22921 gaaaaatgaa gaactcatcc ttccaatgaa caacagcctt tcgttaacat tggatgcatt
    22981 ttatacagag acagaagtca tcttttctga tagctatatg gtagatgaat tttatctaga
    23041 tggcaccttg caagacgaaa aagcaacaaa aaaagtcagt cagtttcttg acctttttcg
    23101 taaagaagcc ggcctctcac taaaagcttc agtaatcagt cagaatttcg ttcctaccgc
    23161 agctggatta gcctcctctg ccagcgggct agctgcttta gcaggcgctt gcaatactgc
    23221 tcttaagctt ggattagatg atctctctct ttcaagattt gctcgacgcg ggtctggttc
    23281 agcttgccga agtattttcg gtggtttcgt cgaatgggaa aaaggccatg acgacttaag
    23341 ttcttacgct aagccagtcc cttccgattc tttcgaagac gatttagcaa tggttttcgt
    23401 tttgatcaac gaccagaaaa aagaagtgtc cagcagaaat gggatgcgtc ggacagtcga
    23461 aacatccagt ttttatcaag gctggttaga ttccgttgaa ggggatctat atcaattgaa
    23521 acaagcaatc aaaacaaaag atttccaact tctcggagaa acgatggaaa gaaacggact
    23581 aaaaatgcat ggaacgactt tagctgccca gcctccattc acttactggt ctcctgacag
    23641 cttaaaagcg atggatgcgg tgcgacaatt gagaaagcaa ggcattccat gctactttac
    23701 gatggatgct ggccctaatg tcaaagtttt agtggaaaac agccatttat ctgaggtaca
    23761 agaaacattt accaaattat ttagcaaaga acaagtaatt actgcacatg ctggtccagg
    23821 aattgcgatt attgaataaa gggagatttt tatgattgaa gtatctgcac caggcaaact
    23881 ctatattgcc ggagaatatg cagttgttga aacaggccat ccagcagtta tcgctgcagt
    23941 cgatcaattc gtgacagtca ctgtagaatc tgcacgaaaa gtcggaagta tccaatctgc
    24001 acaatatagt gggatgcctg tacgttggac aagacgcaac ggagaattag ttttagatat
    24061 tcgggaaaat ccttttcatt atatccttgc tgctattcgc ttgactgaaa agtatgcaca
    24121 agaaaaaaac attcttttat ccttttatga tctgaaagta acaagtgagt tagacagttc
    24181 aaatggccgg aaatatggat tgggttctag cggtgccgtg accgttgcaa cggtcaaagc
    24241 cttgaatgtt ttttacgcgt tgaatttatc tcagttggag attttcaaga ttgcagcact
    24301 agccaattta gcagttcaag ataatggttc ctgcggcgac atcgctgcta gctgttatgg
    24361 tggctggatc gctttctcaa ccttcgacca tccttggctc caagaacaag aaactcagca
    24421 ttctatcagt gagttacttg ccctggattg gccaggtcta tccattgagc cattgattgc
    24481 tcctgaagat ttacgtttat tgattggttg gacgggtagc cctgcctcta cttctgattt
    24541 ggtcgatcaa gttcaccgtt cgagagaaga taaaatggtg gcttatcagc ttttcttaaa
    24601 aaatagtaca gaatgtgtca acgaaatgat caaagggttt aaagaaaata atgtaacgtt
    24661 gatccaacag atgattcgaa aaaaccgaca attactgcat gatttatctg caatcactgg
    24721 ggtcgtcatc gaaacgcctg ctttgaacaa attgtgtaat ttagctgaac agtatgaagg
    24781 agccgcaaaa tcttctggtg caggtggggg cgattgcgga atcgtaattg ttgaccagaa
    24841 atctggcatt cttcctttaa tgagtgcatg ggaaaaagca gaaatcactc cactgccgtt
    24901 acatgtctat agcgatcaaa gaaaggaaaa ccgatgaatc gaaaagacga acatgtttca
    24961 ctagctaaag cctttcatga taaacaaaaa aacgaatttg acttcgtacg gatcatccat
    25021 aaccctttgc cgcaaatagc agtttctgat gtcgatctaa gtacacaggc agttgggttt
    25081 acactaagca gcccatttta tatcaatgcg atgactggcg gaagcgaaaa aacaaaaaaa
    25141 atcaaccaag atcttgcaat cgtcgcaaga gaagcagatt tgatgattgc cactggttca
    25201 gtcagcgctg ccttgaaagt cccttcttta gccgatactt atacgattat gcgacaagaa
    25261 tatcctcacg ggaaaatcat tgccaacatc ggtgcaggga cttctgtcga aagagccaaa
    25321 gaagctatcc gattgtttca cgcagatgcc ttacagattc atttgaatgc acctcaagag
    25381 ttggttatgc ctgagggaga ccgtgatttt accaattgga aagtacttat tcaagaaact
    25441 caaacagcca ttgacgtccc cctcatcgta aaagaagtcg gttttggcat gacaagagaa
    25501 acgctcaatg atctagctgc tttaggagtt catacagttg atatcagcgg tcgaagcgga
    25561 acaagcttca cccagatcga aaacgcccgt cgctccaagc gggaactgag ttatttagct
    25621 gactgggggc aatcgacagt ctcttcattg cttgaagcaa atgaagcaga cacttctatg
    25681 gaaatcctag cctccggagg tatccgcaat gcctatgaca tatttaaagc cctttgtctc
    25741 ggcgcaaatg ccgtgggtac ttcaggaacc gtactgacgc atctgatgaa tcacggcgta
    25801 gaagaaacta ttatactgat gaagcaatgg caagaagagc ttcgtctgct ttatactatg
    25861 gtcggcgcaa cgaataccgc ggccctacac caacaatctc tgatcttttc cggaccagtg
    25921 aaggattggt gcgaagcaag agggatcgac ttagtgaagt atggaagacg cacagaaaag
    25981 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta caaacggcgg
    26041 aagtaaaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
    26101 cgtgaattcc ttcttatttc ttggagttaa atgcttctgt cccagcctct tttttcgaat
    26161 aaatgatgtt taatcagctg tccttcggta ttcgttcaat ccgctgattt cacaacctct
    26221 ccactaaaca gtgtactaaa gctcttttaa ggttgttttc cgatataggc aagtatgcct
    26281 ccgtccacat acaagacatg gccgttaacg aaatcagatg cgctagaagc caagaagaca
    26341 gatggtccag ccaaatcgat tggctcgccc cagcgtgctg ctggcgtacg tccaacgata
    26401 aattgatcga atggatgacg ttcaccgttt tcttgtgttt cgcgtaatgg agcagtttgc
    26461 ggtgtagcaa tataacctgg tccaattcca ttacattgga tattgtattg accgtattca
    26521 gaagcgatgt ttttcgtcag catttttaat ccgccttttg ccgcagcata agcactgacc
    26581 gtttcgcgtc ctaattcact catcatggag cagatattga tgattttacc gccgcctttt
    26641 tcgatcatat ccggaataac tgcttttgaa acgatgaatg gggcatttaa atccacgtcg
    26701 attacttggc ggaagtctgc cgcatccatt tcgatcatcg gtgtccgttt gatgatccct
    26761 gcattgttga ctagaatatc aactgaacct acatcttctt tgatctgttg aaccatcgct
    26821 tgaaccgctt cttcatcggt cacatcgcag acataacctt ttgcatcgat gcctgcttct
    26881 ttataatgcg ctataccttc atcaactgat tcttgattca agttattaaa tacgattttt
    26941 gctcctgctg tagctaaaga gcgtgcaatc tcaaatccaa taccatagac agcaccggtg
    27001 actaatgcta cttttccatc taaacgaaac atatccatac taaattccat cattttctct
    27061 cccttatgct tattttaatt gatccatttg caccatatcc atgtctgtat aggtgatatt
    27121 ttctccgcac attgcccaga tgaatgagta attgcttgta cctacacctg aatggatcga
    27181 ccagcttggc gaaataatgg cttgttcatt gtctacaact aaatgttttg tttcatctgg
    27241 tttgcccatc atgtggaaca cacgtgtatc ttcattggca taatcaaagt agacataagc
    27301 ttccatacga cgttcatgtg tatgacaagg catcgtattc caagcgcttc ctggttctaa
    27361 aatcgtataa cccatttgta attgacagct ttcgcataca tttggatgga tgtattggta
    27421 gattttacgt tcattcagtg tcaggccttc accagtctcc attggtttta cttggtcgat
    27481 actgattttt acatttggat atttatgatg agctggaaca gaactaatat agaatttagc
    27541 tgggttttca gcatcttgag aagaaaatac aacatgtcgt gtttcttttc cgatatagta
    27601 tccgtcttgt tttttcattt cttctttttg cccatcgatc tcgatgaatc ctggtccacc
    27661 gatattgatc acacctagtt cacgacgctc taagaagtaa tcaacaccta gttctttgtc
    27721 taatgtgatt tctaattctt tttcagtagg tgtcactcca ccaaagatca ttcgatcgtt
    27781 gtgcgtataa gtcaaactga ttttccctgg ttcaaatacc ttttctacta aaaattcttt
    27841 tctcaattgt tccgttgaat aatgacggat atcttctgga ctgtgtgtat aacgtgtttc
    27901 catttcttgc attttcttct actccttcta tcgaatgatt tttccagagc cgcatgctaa
    27961 aaattgtgcg acttcttctt gactaaattg gttgcagtcc ccatggatcg tatgcttcaa
    28021 ggcagaagct gccgtagcaa aatcaatgat ttcttgcgga gacatattgg ataaaatccc
    28081 atggagcacg ccacctgcaa aagcatctcc gccacctact cgatccacga ttggggtgat
    28141 tctatggact tgggattcca catattcatg gttcatccac aatgtcccct tcaattcatt
    28201 ttcacttgct gaatgaacgg tacgcttggt cgaataaaac acctgaatat tcgggaactt
    28261 ttcctgcatt ttttgataat aataaattag aggttgttct tctgcttctg gcgctttctc
    28321 gatacctaac aaataaagag catccatttt tccagctgag cagatatcta ctaaaggtaa
    28381 aagctgatgg attacttcgc tggcttcttg ctgtgtccat aattttccac gatagttgat
    28441 atcgaagctg atcaggcagc cagctttttt tgcagcttct atcacctgtt tcgtcatttt
    28501 ttgccatttt ttagacaaag ctggggtgat tcctgaaaca tgaaatatat ctattccttg
    28561 gaataactcc tctagcgacc attccaaatg atccatttca gcaaaacttg atccggcacg
    28621 atcatagatc actgaggcgg cgcgttcgcc actaccagtt tccatataat aagtacctag
    28681 tcttgatcca ccagataata cctgtttcgt atctactcca taacattgca agtgcttttt
    28741 gactgctttt cccaatgcgt tatcaggtac ttttgtagca aaaatcgctt catgtccgta
    28801 attagctaaa gaaattgcta cgtttgcttc ccctccgcca taatgggcag cgaatcgttc
    28861 acttgaatcg atacgggttc cctgctctgt ggacaatcgc aacatgattt ctccgagtgt
    28921 cactactttt cccataagca gctacctctt gatttctttc atttttgctg catactgttg
    28981 cgcgacttcc gttactttgt caaaatctcc tgtagctgcg ggtgctaaaa ggttcccacc
    29041 tactccgact gttacgacac cagcatcgaa ccactctttc atattgtcca gactcacacc
    29101 gccagttggc atgatattga cttgaggcat cggcgccttg aaggcagaaa tgatacttgg
    29161 tccataagcg cttccaggaa atagtttgac gatatctacc ccgcttttca gcgctgtttt
    29221 gatctcggta atagtcatac atcctggtaa gtaaggaatt tgatacagat tgcacatctc
    29281 tgccgtttct tgatcaaagg aagggctaac aatatactct gcgccagcta agatagccaa
    29341 gcgagctgtg acagcgtcta atactgtgcc tgcgccaatc actatttctg gttggtcttt
    29401 gtagaatgac aaaagttctt tgattacttg atctgcttga gggactgtaa aagtcaactc
    29461 aataccagtt agaccacctt tgatgattgc gtggcaagca ttcaaagctt cttctttgct
    29521 ttttccgcgt accaccgcaa tcactccggc gtttttcaat cgagataaaa tatcaatttt
    29581 tttcatcttc tccacctatt tcctatttag aaattttatt ttataacttg gaaatctata
    29641 cccatattat acggcagaaa agaaaaattg caaacgcttt tttcattatt tttgaattct
    29701 ttgctcgatt tcgctttttg tttgcttgag ggcactgatt atttgttctt ttgtctcatc
    29761 tgttaaacga tatttcggca tacttacact aaatgcacca atgatttgtt cgtttttcac
    29821 gattgaggct cctatacaaa aaatatcacg ttcgatttct tcatcgtcaa atgcgatgtg
    29881 ggtttctttt accgttttga tttccttttt caaccgtaat gcattggtga tcgtatattc
    29941 tgtgtaaggg atcaacggat gcttttcaag ataatcagtg tactgctctt cactgaattc
    30001 tgctaatacg gctttgccca tcgcagaatt gtataatggt cgggtgattc cgatttttga
    30061 agacatgcgg atcgtttgat gttttggatc tagtttgttc acgtacaaga tctcattgtt
    30121 gtccaatacc cctaagtgga tagtctcatc gatcttttgc tgcagctgtt ctaaaaacgg
    30181 taaagtcaac tctaccaaat cgatttgttc aatgttttta ttcgcgtagc gaataagctt
    30241 tgcacctaaa cgatagtttt tctctgaatt tttatttacg tagccgatca tcaacaaagt
    30301 atctagtatt ttcaagactg tagaagaggt cattcccaca ccctttgcga tcgtctgcaa
    30361 agagacatct tggttttccg ctaaatagtc tagtatttta gaggctttga tcaatacagt
    30421 tccataaggc tttttatttt ccatataata ttctcctatt tctttataag aaaaattata
    30481 gcggaaatac catcaattta aaagagatat ccagatataa actaccattt ctctttacag
    30541 gcaatttaat gttgaagatc cgcttcgttt ttacatcaaa ctttgatata aagcaatgat
    30601 ttcttctact gtcgtttctc ttggatttcc tggtgtgcat acatctcgca aagcatcttc
    30661 tgcaattgct ggaatatctg cttcttttac acctaattca gaaatggttt ctgggattcc
    30721 aaccgctttg ctcaaccgat cgatctcacc acaagccgct tctcttacat cttctaaaga
    30781 catttcagct gctcctttaa tccctagaac aagagcgatc tcacggtact tttctccagt
    30841 atattctttg ttatatttca tgacagttgg caaaagtgct gcacacgcta cgccatgagg
    30901 aatgttgtac catgcactta acgggtgagc catcccgtgt accagtccta aaccgacatt
    30961 tgagaagccc attcccgcaa tatactgtcc taatgccatt ttttctcgcc cattttgatc
    31021 gccatctaca gaagcttcca aagaatggcc gatgatttcg attgctttta aatgcagcat
    31081 atcagtcatt tcccaagcgc cttttgtgat aaacccttca atagcatgcg tcatagcatc
    31141 catacctgtt gatgcagcca attttttcgg cattcccatc atcatatcgc tgtcgatgaa
    31201 cgcgacgatt gggatatcat gaggatcaac acaaacaaat tttcggtgat ttacttcatc
    31261 tgtgatgacg tagttgatcg ttacttctgc tgctgttcca gaagtcgttg gtatagctaa
    31321 tataggtaaa catggatttt tcgtatcagc aacaccttct aaactaatga cgtctgaaaa
    31381 ctctggattc gtcaaaataa tcccaatggc ttttgccgta tcgattggcg aacctccacc
    31441 gattgcgatc aaacagtctg cctctgcctc tttaacaaaa gccactccat cttttacatt
    31501 ttggatcgtt ggattaggta cgatcccgtc gtataaagca taagcgattg ctgctttatc
    31561 tagaagttct gttacttttg ttgctatacc gtgctcgatc aatcctttgt cagtaatgac
    31621 agctgctttg ccgaatcctc gctgctgaaa ttcagggacg atcgactcaa tcgctccttt
    31681 tccaaaatac gatgtttcat ttaaaatcat tcgattactc attttctatt cctcctaatt
    31741 tgtattattt ttcacaataa aaacgaaaga accccaagca accggttgcc cagttgcttg
    31801 ttgctacagt tcaaagactg gcatcaattc ccaactgact ggagaggcat ctggatttgt
    31861 ctccatgata tcttccatgt acttccacca tttttgattg atagatgtat ttgccatttt
    31921 ttcccataac ttttcatctt cgatcactaa gtaaccaaat aacaaacttg tactcggatc
    31981 aagatagatc gaatagtcga tgaccccgtg ttcacgaagt gctgtacgca tttctggcca
    32041 aagttcatca tgtctttttt tgtattcttc ttgtttttct gggtaaactt tcatgcaaaa
    32101 agcttttttc accatgtcag acacctgctt tcgtatgaac ttcctccaaa taacctgctt
    32161 ttggagtgac accaaacgca tctgctaaat gccacaggtt ttcgtccgtg attgtttgca
    32221 agattggtcc ttgcgctttg acgtacgtat agacttctgc tgccttttct gctgtctcga
    32281 tcaaaccaaa gacttcatcc atgtcttttc ctgacccgta gattccatgt tgtggccata
    32341 aaaccaatcg agtttctttc attttttcag ctgttgcttc cccaatctca tttgttcccg
    32401 gaaccatcca cggaatgatg gaaattcctt ctggaaaaac aactaagcat tccgtacaca
    32461 tttgccatag cgtacgacta aaggaacgtt cgtctaattc atgcgtaaaa ctcatcgcta
    32521 atagatgact agcatggcaa tgcataacga tccgattatc tgggtctact tctaaacggg
    32581 cgatatgact catgaaatga cttggcaact cgcttgtagg aaggcattcg ttttccagcc
    32641 cccacaaaag ttccagtgtt ttgccatctt cagcaactcg aacgattcca agattctcgg
    32701 caggttcgct cgctacattt ttaaaatatc ttcctgaacc agttacaata aaatagcatc
    32761 ctgctaaacg gctggcatca aaaatcatcg agatgcggcg gatcactttt tcaggatcca
    32821 agaacggtgt gatctcttct tcttttagta aataagaaat attcccgcca tttctttcat
    32881 cccatcccaa gcgataaaga ttcgacgtcg tttcgatcat ttctttcaca taaggcgctt
    32941 gtaaaatatc tatttttttc aatatagcca cactcctatt ttcttaattc caacacgtct
    33001 ttttgatatt cctggacttc ttccagccaa tccaaaccaa ctggaacttg atttttgtaa
    33061 cagaaataat tccatacatc ggcatacggg aaatctttca attcttccgt ataagccatt
    33121 cttgaagtaa agtcaaaagc cagctccatt tcttccaatc gctcaactgg ttccagcata
    33181 gctttcagca gtgctttttg tgtatttctc gtaccgatga cccaagcagc gatacggttg
    33241 atcgttgcat caaagaaatc cagaccgata tttgtttttt ccaacaattc gttacggact
    33301 aattctttag cgatttcctg caattcatca tccatcaaaa ccacatggtc tgaatcccaa
    33361 cgcactggac gggaaacatg caacatgatt cctttgctga ataaagctaa tgaagataat
    33421 ttattggaaa tcacttctgt tggatgaaaa tgtccagcat ctagacaagc aagtttgtca
    33481 cgagtcaacc cgtaacccat ataaaattca tgggaaccga ctgtataagc ttcagcgcct
    33541 aaaccaaata gtttgctttc tactgcatct tgtacatacc gttcatctaa ctcttctgta
    33601 aaaatttcat cgagtgattc cattaaacgt tcccgtgggg taaaacgatc aatcggatta
    33661 tctttcatcc catcaggtac ccaaaagtta ttgatacttg tttgcccagt ttctttccca
    33721 aagtactccg cgattttgcg gcttcttttc ccgtgttcga tccagtattc acgaacttct
    33781 ttatctggat gagccaatgt aaagccgtct ttcatcattg gatgcgaaaa gaaagtcgga
    33841 ttgaagtcaa gaccgatttc ttcttttttt gcccactcta cccatttttc aaaatgtttc
    33901 ggctcaattt catttagatc gacagattct tctgtatcca aatagatagc atgaaggttc
    33961 aatttgtgtt tgcccggtat caacgaataa gccttctcca gatcttgtct aagctcttca
    34021 ggtgttcgtg cggctcctgg aaaattccct gtcgccatga ttccaccggt caattcgcca
    34081 tccggtgtca agaaaccttt gacatcatct ccttgccagc aatgcataga aattttgatg
    34141 tcctgtatct tttctagtac agcatctgta tctacaccga tagttgcata tttttctttt
    34201 gcttgttcgt aatttctttc aatcgtgttc atacgattac tcctttgatt ttattcacgt
    34261 accattccat cgtgttttgc ttattttcat ctgggtaaaa agtttcacat ggaaacgact
    34321 gtttgattac atttcttgct tgttctatat tcgaaaactg acctgtactg agcatttgca
    34381 tggcaacatt cccgattgct gtggcttcac taggtcctgc ttctacaatg attccggcat
    34441 aatctgctgt caattgattc agtagacgat tattcgatcc gccccctacg atgtgcagct
    34501 tttccaattt cgttccagtt aagtgttcta ctttgatcag ctcttgtgcg taacaaagag
    34561 caagattatc atacacacaa cgagcgatag ctcccgatga gacagggact ggctgctctg
    34621 tttcaaaaca gtattgctga atctcttcta tcatatttgg gggattcaaa aatcgagggt
    34681 cattgatttc catcacttgc tgcagcggtt tttcttttgc agcaagctct gccaattccg
    34741 cataactgta ctcgtactct tgcattcggg caacttcctg gatcaaccac atccccatga
    34801 tgtttttcaa aaaacgcacc gtgttatggg caccccattc attcgtataa ttttcagaaa
    34861 aagcattgcg agaaacattg gcgatctttg tttcaatccc taaaagcgac catgtcccac
    34921 tgctgatata accccaacgt tctccttgac ctggcgttcc taatacagct gacgccgtat
    34981 catgacttgc tactgtgata aaggtcacat ctggcaaatc atattttgaa aattgttctt
    35041 tttgcagatc tcctagtatc gttcccggct ctgtcaatgg tggaaataat tccttcgaga
    35101 caccgatttt ttcaagaagt tctatttccc attcttttgt ccctgcattc agcagctgca
    35161 tagtagatgc attagttttc tccgtcacca tgttaccagt aaagacatac cctaaataat
    35221 caggtatcag taacaattta tgtgcttttg ccaaattttc tttttcttca gcaaacaatt
    35281 ggaacaaggt attgaaaggc tggatctgaa tccctgtctg ctcatacaac ttatctagag
    35341 aatagatttc agaaaactgt tctactgctc cattggtacg cttgtcccga taagcaatcg
    35401 gatcatttat ccgatcccct tgttcatcaa tcagacagta atcgactgcc catgtatcga
    35461 tccccgcaaa aaggcgatca atccctgtct gctttaattt ttcaaatcca atcaaaatct
    35521 ctttgataag ataatcgatc ttccagcgat cgtgaccgtc tttgttccga aagccgtttt
    35581 tgaaccggtg tacttcttcc aagatcaaag aaccttttgc atccctcgta cttttcatta
    35641 gtcgtccgct tgatgcaccg atatcgatgg caacaaatgt tggattcatt tctccacctc
    35701 ttttacttta ttgttgatta gcactttatt aataaagcgt tatcatttat tgacaatgct
    35761 atcataatac agaatcttat attttgcttc acactatttt ggtatataat tgcattatct
    35821 tgacaagttg ggagtgttta catggattac tgggaaaaag ccaaccactc atggtcggaa
    35881 gactcaaaaa gagtgatctt gacgcctaca caaagaagca ggaatttatt tttttatatc
    35941 caagaaatcg gttacttcaa agcaccaatc ccttactata ctgaacgagc acatttacct
    36001 tcttacttga taaagtttac tttgtctggc aaaggttcgc ttacatataa tgataatcag
    36061 caacagctaa aagctggaga cttatttttt attgactgcc aagaatatca gcgttatcaa
    36121 acgatcagcg aagaaccatg ggaaatggat tggatccatt tgaatggtca gaacgtccaa
    36181 tcattttatc aggagttcac aaaagacggc cataacacct tccattcaac aggagatcca
    36241 tcagaaaaca agatccacca tttgatcaca gaattgatcc acatgcaaca aggcacgaat
    36301 gcccgtaccg atttccagtc ctctgtagct atccacgagt tgctgaatga actgattttg
    36361 caaaagtatc agctagattt cgaagaagag gatattcccg cctacgttta caaactgaag
    36421 caatacttag acgaacattt caaagaaacg attacacttg atcaattaga acaacaattc
    36481 ttattgaata aatatcagct gagcaaagat ttttccaaat ttatcggttc tccaccaatc
    36541 gattatttga tcagcaaaaa actttcttac gctaaagacc ttcttcggta tacagaattt
    36601 acggttcgaa tgatttccga agaaataggg attgagaatt ttgcttactt tagccgcctg
    36661 ttcaaagcaa aaacaggact atcgccgaga gactttcgta aattcagctg attcatataa
    36721 tatgtacgtc attttttcaa aatattttga aatatataga taaaaataaa ataaccgcag
    36781 tttctgcttc tatgtaaaca acagcagcta ctgcggttat tttttagtga tcattctcat
    36841 tgttcatctt caaaatgttc ataatgaacc aactgtttct gtgtatcacg tttcaaaaat
    36901 tctttaggcg ttatgccgaa ctctctcttg aatgctttca gaaaatgcgg ataagaagaa
    36961 aaaccacttt tactatatac agcactagag ctgcggtatt ctcgcaacaa gtgcttggca
    37021 tagatcagct ttttcttcat tacatactga tgaaacgtaa atccagtgta ctttttaaat
    37081 tctcgtgtga tatggtattt gctgacaaaa aactgctttt ccatttcctc taaagaaagc
    37141 ggatgagata aattttttga gatatattgt aacatttgtt ccacacgctc attaggaact
    37201 acagcattct gttcttgcgc actctcttct ttttgtacaa cttgattgag aaaaatcaag
    37261 tattctacca gagcttgttc atattttatg tcttcaccgt aaattttttt atcaggagga
    37321 tggtcgataa atgataattt ttgcttcaat acttcctgtt ctatttgcaa tacacgactc
    37381 ctcttctctc ccattggttg aaaacaagcc gatagattag accatggcgt ggaacgactt
    37441 tctagaaaag aaggggtgac aaagatatag acacgctcga atacatctga agattggcgg
    37501 acgatacgat gcaaatcatt ggaatggatg agcagcacac ttcctgcttt gatcgtaaat
    37561 tgtcgtccgt tcaaataaaa agtcgcttct ccctttaatg tagcatggat ctcatagaaa
    37621 tcatgatgat gatagatcgt tttattttgg ctgacgacat ctttgacatg atagatttca
    37681 tactgatcag aaaccatcgt attgacttgt tcaccgctga ttttcttttg gagcatgtgc
    37741 cgcacctcct tttgttcact tatgcacaat tttattatac cacgtttttt gtaatcaaaa
    37801 agagctgctt ttatatactc ttataaaaaa gaagagacgt ggcatcttct agccccgccc
    37861 cctccattct aaatattagt taaccaacgt cttctttaca ttattcaaga agacttcttc
    37921 cataacgaat cccatcatct tttgtttctt caagaaccac atacaggtac ggacgattct
    37981 tcatgatgat ttctttgatt tttgtgtact cgatcacacc gtctcccaaa tttaccggca
    38041 cgattttttc ctgttcgaca cgaaaatctt tcaaatgaac cgcacatatc ttttcaccaa
    38101 aacgttcaaa agcttcttct accaattgga cttgttcttg ataatcacta cttgtgatca
    38161 aattggtcgg atctaaaatg attcctagat agtccgaatc tactgcttgg atcagctgct
    38221 ctacccttgc taaagaatac accggatgat tcaaacctgg ttctatcccc accatcattt
    38281 ggtatttttc tccagctttt accaagcgac ggataacgga cacagcttca gcaaatgctt
    38341 catctgtgaa attttcttcc gtatattgga tttccggcaa aacacatcca gtttccgaag
    38401 caaccatcga cgcaccaaag gaagaagcat agcgaagata ctgctcgaac tttgtcagca
    38461 actcttgtcg tatcgttaaa tctggatgaa tcatattgat atagcaactt aaaattccta
    38521 cggtcacttc ttgcttttct aaaactcgtt tgacataatt tcccatccca gaattgatct
    38581 catttgcgcc agaaggtatt tcaggaaacg aaagacctaa ggcgagctgc agtgtatgaa
    38641 tcccttgttc ttttgttttt tctgcaagat cttctatcgt ttggacttcg gaaagatcat
    38701 gtcctctaat tgtaatgttc aacataacta ttctcctaac tcagcatatt gggtagccac
    38761 atgacgattt gcgggaagta agtaatcaaa aacaagacga ctaagataac cccataaaaa
    38821 ggaagaagcg gtttcagcat tgcctctgct tttacgcctc ctacacttgc accgacatat
    38881 agtcctgtac ctactggcgg cgtgatggag cctacacaca gattcatgat tgaaaacaac
    38941 ccgtaatgta caggatcaac ccctacactt gtgacgattg gtaaaaatat tggtgtaaaa
    39001 atcaagatag caggcgccac atccataaac atacccacca gtaataacac aagattgact
    39061 aataacagaa taataaattt attatccgtt acgcccataa tcaacgaact aattgcctga
    39121 ggaataccgg taaacgccat ggcgaaagac atcatggaag aagtagccaa taagaacatg
    39181 atcgtccctg acattgctac tgattgaaac aacatattag gtatatcttt tagttttacc
    39241 gttttatagt aaaacatagc aatcaacagg gagtaaatga cagctatcgc tgaagcttct
    39301 actgctgtaa atatccccgt caagatacca ccgataatga tgacaatcaa tagaaggcta
    39361 ggaatcgcct cttttattat ttttccagca tgagccatct gtcctttttc gacaacaggg
    39421 tatttattct ttttagcaac gatgaatgct actagcatga ttcctagcgc ccacaaagcg
    39481 cctacgagat aacctcccat aaacaaagca gagatcgaag caccaccagc aacaagtgaa
    39541 tacatgataa aagcagtact tggaggaatg accatacctg tcggagctga cgcgatattg
    39601 accgctgtcg caaacttccg gtcatatcct tcttccactt gctgagggat caacactccg
    39661 ccaatcgcgg tagaagcggc aatggccgag ctagagatgg aaccaaataa agcattccct
    39721 aacacattcg tatgagccaa agcccccggc acgcgcccga cgaacaacat cgcaaaatcc
    39781 actagtttct tagctatccc tccattattc atgataatgc ccgagagaac gaaaaacgga
    39841 atagcgatca gcgaaaaact attgatactt cccaccattt tttgagctga actaaaagca
    39901 gaaacatcaa acggtataac taacaataaa gtcatcatcg atgagaatgc aatacttata
    39961 gcaatcggca tccctaatac aagcaacaca acaaatacaa aaaataacac taatccggct
    40021 tctattgcca ttttattcct ccatttttat attttttctt gaattgctgg cttgttgctt
    40081 aatgtatcga ttagattgat caggctatag ataatgatga gtacccccga tacaggtact
    40141 gacagataaa catatcccat aggaatactt aaagaaggcg agatctgccc cattgtcaat
    40201 gtgacagcat tccatccgcc atacaccatg atcatcgcag caaataccag aaataaactt
    40261 tgaacaacaa cttctaaaat tttacttttt ttgccagtta atttttcact taaaaccgtt
    40321 acggctaaat gtgctttttg tcccacaaca tacgcggaag acaacatggc aaaccagaca
    40381 agaccgaatc gaacaagctc ttccgtgacc gtatttggat ttcctaaaac cgttctggaa
    40441 aatacttgcc ataaaacaac agcgaccatc ccaacaagca aaacagctcc caggatctct
    40501 aataccttgt ttaaaatcat tttcatcaca gctacgctcc ttgctcttgg atcttttgat
    40561 aaaggtcttt gtaggtagga tcgtttttga atatatcatg aagtggggca actgcctctc
    40621 gaaacggtgt attatctaca tcattgaatt ggactccatg ttcttcttgt gctaaagctt
    40681 tttcttcttc aattgctttt gcaaaagctt caatttcaaa aaccgttgat tctttcgctg
    40741 cttcttcaat ggctttttcc tgctcatcag ttagctgttc tttgatggca tcattcatga
    40801 tgatgatatc tggcacacgt gtatgctcat cataggaata gtatttagcc acaccaccat
    40861 gtccagctgt atacaagaca aattcattgt tttctgatcc gttgatcaaa ttggattgaa
    40921 gagacgtgta cacttcatca cttcccatag gtacgggcga accgcctaaa agctctacca
    40981 ttttgatagc ggtttcgctt tgcatcacac ggatcttctt tcccttcaaa tcttccggcg
    41041 tatggatcgg accgtctacc atataaaagc tgcgctgacc agaatcataa taggtcaatc
    41101 cggtaaatcc taaatttttg gtcgattggt agattggctc cattatttct tcattatcca
    41161 tcaccttaaa gaaatgttct tcatcattga atagataagg aactgaaaaa atcgaataat
    41221 ctttagagaa actttccaac gctgaggcac ttacttttgt gaaatctact gctcctgact
    41281 gtgtcaattc gatcagttca cgttctccac ccagttgtcc atctgggaaa tactggatct
    41341 gtacggaacc atctgttttt tcttttacta aacgcccaaa ttcattcatt gcatcaatta
    41401 ctggctggct gttgcttgca taagcaaagc gcaaggtaac tatctcattt acatctgcat
    41461 ctgcgacagt tgccacacta gaattatttc cgcagccgct tagacctagc agacctaggc
    41521 taagcgaact tgctgcaaac agcagtgatg tttttttcat atgggtttcc tttcttttaa
    41581 caataagaga cgagcaattc tgtcaaacaa agcaccgtta acgactgtcc ataaggcatt
    41641 gctgtgattc cgatattccg ataaaaatct aaactgtccc ccattcctgt cccgattgaa
    41701 acgttttgaa cttctccttt ttcatcgatt tgttcaagca atccttggat agcacgataa
    41761 gcgacttctt tatattcttg tggtaaataa cgtttatgga ccgctttcaa aataccgtaa
    41821 gcaaatcctg cagttgcaga agattctacg taagaagaag gatcatccaa caacgtatgc
    41881 cataaaccgc tttcagactg ataagttttt aatgcacgca cttgtgcttc aagtgtttcg
    41941 atcaataatt tgcgcaatgc atcttctttc gataattcta agatttcaat gatctctgga
    42001 atagcaatcg tcaaccagca gtttccacga gcccaaaatg cttcggcata gttatgattt
    42061 ccttcaaacg tccagccgtg ataccacaat cctgttcgtt tatccatcaa gtatttgata
    42121 tggatcataa actggtaacg agcttcttct aaatattctg ggcgattcaa cagtttcccg
    42181 atttttgcca aaggaagcac tgtcatcatc aatgtatcgt cccacaactg attcttattt
    42241 tccggtccat atgtggcatg ctgtaacccg tcttcatttg tccgaggcat gtcattcatg
    42301 acccattctg cccattgttc taaataaggc aaatagcgtg aatctttcgt gtcttcatac
    42361 aagtaagcca tagtcaatag cggtgccatt gtattgacgt ttttgggcgg agcgccttct
    42421 tccatacgat cctcaaacca ttcattgacc acttgataag cgcgaggatt ttttgtcatc
    42481 tggtaatttt tataaatacc atatagaccg acaccttgtg gccaattcca cactgtccag
    42541 cttttgtcat ctactttcag tccatcaaag tccaacaaaa attcaccatt atcgtctttg
    42601 atcgttgtca ggttttcaac gagcaggtca attttttctt tcactaattc tttaggtaca
    42661 aaaatctctt ctttaataat catctttacg ccaccttttt aaacgctttc attttatatc
    42721 cttaatatag cagtaatacc gtccactata aatatcgctt tttgaacaaa aactgtctaa
    42781 aattgaccta aatgttaaat aacaaaagta actttcagtt acttggaaat taaaaaaaga
    42841 acttggagaa acaacgtttt ccaagttctt tttcatctat tttcttatct tttttctacg
    42901 ctttcttttg cttatccgtt gtaagcatca atgatgatct gtttcaattc agagatcaat
    42961 ggttctttcg gattagctgt cgtacattgg tcttcgtaag ctaattcagc catacggtct
    43021 acggttgtat ctagtgtttc ttgtgtgact ccttgagctt ttagactcat gttgatgccg
    43081 acagattttc ctaattcata aacagccgtt gctaaagctt cgactaattc ctctgttgtt
    43141 tcgcctttca gacctaagaa ttttgcgata tctgcgtaat ctgtatctgc acggaaatag
    43201 tcatatttag ggaacatcgc atgtttttgt ggatcttttg cattatagcg gataatatgc
    43261 ggtaaaagaa tcgcatttgt ccgtccatga gggatcccgt attccccgcc gattttatgt
    43321 gcgactgagt gacaaatacc taggaatgcg ttagcaaacg ccataccagc cattgtagaa
    43381 gcgttgtgca ttttttcacg tgattccata tcaggtgttt ttactgattt ttctaaatag
    43441 tcaaagacta acttgattgc ttgtaggctc aatccacgtg tataatctga agccatgact
    43501 gaaacatagg attcgattgc atgtgttaac acatccatcc cggtatctgc tgtgacagat
    43561 gctggaactg acatgacgaa ttgaggatcg acgattgcga catctggcgt caatgcataa
    43621 tctgctaacg gatatttcac atgtgtttca ctgtcagtga tgaccgcaaa tggcgttact
    43681 tcagaaccag tccctgatgt tgtcgggata catacgaatt gtgttttttc tggtttgtcg
    43741 atcttatatg tccgtttacg gatatccaag aatttttgtt ttgcaccaaa gaaagatgtg
    43801 tctgggtgtt catagaacat ccacattccc ttagctgcgt ccattgcaga accgcctcca
    43861 agagcgatta ctgtatctgg cttgaagtct gccatcagtt tcgttcctgc gtatacggta
    43921 tttgtcgatg ggttcggttc aacatctgag aagacttcga tcttcacatc atttttacgt
    43981 cgtgccaata cttcacgaac tgtatcgcag taaccgaatt ggaccatacc agggtcacaa
    44041 acgatcatga cacgttcaac gttttccatt ttctctaagt acaatagaga gttcttttca
    44101 aagaagattt ttggcggtaa tttgaaccat tgcatgttat ttctccgttt cgctacagtt
    44161 ttgacattga ttaagtttac agcagagacg ttacgagaaa cagagttctt accataagaa
    44221 ccgcatccca aagtcaatga cggaatcatt tcgttataga tatcaccgat gccgccttct
    44281 gcagatggtg tattcactaa aatacgacaa gctttcattc gaagaccgaa tttgacatgc
    44341 aactcttcgt tttctgaatg gataactgct gtatgtccta atcctccaag attcaacata
    44401 ccttcacaca aatcaaacgc atggtccgtg ttgttagctt tgatcatggc aagaactgga
    44461 gaaagttttt ctcttgatag cggataatct gggcctacgc cttccaattc agcaatcagc
    44521 attttcgttc cttttggtac actgattcct gccaattttg cgatttccat cgcagaatgt
    44581 ccaacgattg atggattgac cgcatatttt ccttcgttca tcactgcatc ttctaatttt
    44641 tgcaattcat ctggttttac gatataaact tggtgtgctt ggaattctgc ttttactgct
    44701 gcatagattt ctttatccac gatcacagct tgttcagaag cacaaatcat tccattatca
    44761 aatgttttcg aaacgatcaa atcgttcact gcacgtttaa tttttgctgt tttttcaata
    44821 taagctggta cgttacctgg acctacacct aaagctggtt ttcctgtaga gtaagcagac
    44881 ttgaccattc cagctccacc tgtcgctaag acgatcgcga tacctggatg gttcattagc
    44941 atagaagtcg cttcgatcga aggatgttcg atccattgga tacaattttc aggagctccg
    45001 gcagcgatag cagcatcacg taccactcgt gctgcttctg cagaagattt ttgggcactt
    45061 ggatggaaag caaaaacgat tggatttctt gttttcaacg cgatcattga tttgaaaatc
    45121 gttgtggaag tcggattcgt tgttggagtt actccgcaaa ccacaccgac tggttcagca
    45181 atttcaatca accctttttg gacatcttca ttgatcacac caactgtttt atcatgtttg
    45241 atattatgcc aaatatattc ggacgcatac atatttttga ttgctttatc ttcgtaaaca
    45301 ccgcggcctg tttcctctac agccatcttc gctaatggca tatgttgatc caaagctgcc
    45361 attgccattt catgcacgat atggtctact ttttcttgat caaagctttc catttctttc
    45421 aatgctgcat ttgctttttc tgccaaatca tcaatcattg cttgtacgtc aattgtcttc
    45481 gttttttctt tttctattac tttagccatc attatcctcc taatgggtca tggttatttg
    45541 ttaaataatt cacatgtata tcatacaata tttatttttc tatgtaaaca cttttttgtg
    45601 atttttttca cagttatttt taaatataaa attgctttat ttacaggtgt ttgtatagaa
    45661 aaatatataa agttatttat tattatttat atgacacacc cgattattca atattgagca
    45721 atatatccca tgctcttatc tctttctttt ttgaataaaa aatgaggtag gacatctttc
    45781 tgccctacct cgtctttaaa taagctttga tacttatttt tcttccttct cttctgtctc
    45841 ttccagcgtt tctgttgttt cagtttttac ttcaactgtc tcagctggtt cttctgttgt
    45901 ttcaacagta gtcgttgcat catttgttac gacagtccct ggtttgaccg ttttgattgc
    45961 tgcacgatcg tactcaagga agatcccttc gcagtctaaa acaactgtcc gtttgtcatt
    46021 gtcgatttct gaaacaactc catgtaatcc gccgatcgtt acgactgaat cacctggttt
    46081 catgctatct aacaagtttt gacgttcttg ctgttgtttc ttttgcgaac gtgacataaa
    46141 aaaccacatt gctaatagcg cgataaacat cacaagcatt ggtaatgttc ccattttttt
    46201 cacctcacta attctgtatc tacaaaaaac actttagcag aaaaatgctg ataacactag
    46261 ttttttactt aatttttaaa agattttcct ttagaagctt tttgcgtttt ctttattgaa
    46321 cccgtattct tcaaaaaagg cttctctaaa ttcaagcaaa ttatcatcca tgattgcttg
    46381 acgtacttgt ttcattagat tcaacaagaa gtataaattg tggtaagaag tcaaacgaat
    46441 cccaaatgtt tcatcacatt tgatcaaatg gcggatatag gcacgtgtat agtttttaca
    46501 tgtataacaa tcacatttct catcaattgg acggaaatca tgggcatatt tcgcattttt
    46561 cactactaga cggccttgag aagtcataca tgtgccattt cgagcgattc gagtaggcaa
    46621 gacacagtca aacatgtcta ctccacggat aacaccatcg atcaatgaat cagcagcacc
    46681 gacacccatc aagtaacgtg gtttgttttc ggggatcaac ggtgtagtga attctaatac
    46741 acgattcatt tctgatttag gttccccgac agataacccg ccgattgagt aaccagggaa
    46801 atccatcccg actagatctt tcgcgctttg acgacgaaga tcttcaaacc ctgctccttg
    46861 gatgattcca aataatcctt gacggtctgg attagcatga gctttcaatc ctcgttctgc
    46921 ccatcgagac gtacgttcta ctgatttttt cacgtagtcg taactttcat cgaatggtgg
    46981 acattcgtca aaactcatca tgatatcaga gcccaattta ttttggatgt tgattgcttt
    47041 ttctggtgat aaaaacatac gcgaaccgtt caagtgattt ttgaaatgca cgccttcttc
    47101 ttcgatattc cgcatatcac ttaatgaaaa aacttgaaag ccgcctgaat ctgttaaaat
    47161 cggttgatcc cagttcataa atttatgcag tccgccagct tcttctacta agtcttcacc
    47221 agggcgtagc cataaatgat atgtattact taaaataacg ccagcaccca tttctttcaa
    47281 ctcttctggg gacattgttt ttactgtagc taatgttcct actggcatga acattggtgt
    47341 ggggaaagtt ccgtgtggcg tgatcaattc ccctaaacgc gcgccagtat gtttttcttt
    47401 cttgattaaa cgatagcgaa tggcaggttg cgtcattcat ttccctactt tctatataaa
    47461 atatcttgac aactcccacc atcataatcg tttcaagtgc tttttgcaag ttccctcggc
    47521 ttccttctac tttatttaat attctttttt ttatcctttt tttatgaata acaaaaaaaa
    47581 gaaaaacatt atgacagaaa tattttttga tacatggtat attggtgtta ttaaagaaaa
    47641 ggagggaaaa atcatgcaat cttctgcaga attaaaacgc caagcaaaag agagtctaaa
    47701 aggacgttgg ggacaagctg ttttactaaa tctaatccct acattgattg gtattgctac
    47761 tattcttctt attatgattc ctattatctt gtttgccacc accatatatg atggatcagc
    47821 gagcgtctca gaagcgacaa cttcaggagg cggtagttca ggaggcggtg gcggaattat
    47881 ttccagcatc attagcgctt tgttcatgag cggtatttct tggacttatc ttgatatttt
    47941 acgtggacaa aaagatcaga tcgaaccatt caaagatgca tttcgtggat tcaaaggttt
    48001 gtttatcggc ggagtgatca gcttagctgt gctgatctcg atctttacaa cattatgggg
    48061 tttgctatta gttattcctg gaatcattaa aagctattct tattctcaaa gctattttat
    48121 ttactacgat atcgtccaag aaacaggaca aacacctaaa gtactggata cgatcactgc
    48181 aagtcgaaaa ttgatggatg gttataaagg acaacttttc tggttagatc tttcatttat
    48241 cggctggcat atcttagcat tagcaactgc tggtattggt tatctatggc tcagcccata
    48301 catttctgca acaaaagcag cattttatga acaattgcca aaagatattt aaaagagagt
    48361 gtgacttgtg accgaggggg tggcaggaga acaccgttta tcgagaggca gtaaaagaaa
    48421 cattcagccc attagcaaaa aggaggtgtg acaacattca tgtcacacct cctttttgta
    48481 tagttccagt aaaaagaaaa tcaacttaga aacagttaat cattcctctt cttctgcttc
    48541 cggtaatcga tggaacttga tcagttcatt caccgcgatt ggtacgatag ataaaatccc
    48601 taccaagaac cagtgattca aactgatgtg tactaggccg aatagttctt gcgtaaatgg
    48661 cagcaaagca acagccgttg taatagctaa tgccagtaac gccatttcta ccaaagcttt
    48721 attggtcgct aaattgactt tgaagatcga ttggctgctg cgtacattga acacatgcag
    48781 aatcgatgaa taagctaaga tcaagaatgc aacagtttga ccgacatgct gacttgcgtc
    48841 tacccatggg ctgatagtat cgacataact acctaaataa taaccaaaca aggttacgat
    48901 ggtgaagacc cctgcattta tggcaatttt ttgcaataaa cctcgagcaa agatcccttc
    48961 atttttcgga atgggtggct gatccatgat gccgtgttcc gctggctctc ttcctaaagc
    49021 aaatcctgga agtccatcag caactacatt gacaaacagt agttgcacag ctgtaaatgg
    49081 tgctccccat cctaacagca tcgcaatcaa aacaataaag atctccgaaa tattacagct
    49141 tagcaaaaag ttgatagctt tacgaatatt ctgataaacc gaacgtcctt gactgacagc
    49201 gtctacgatc gtcgcaaagt tgtcatccgt caaaatcata tctgcggcac tttgcgccac
    49261 atctgttcct gtgatcccca tggcacaccc aacgttactt gctttcaacg ctggtgcatc
    49321 attcacacca tccccagtca tcgctacgac tgcacccgta cgttgccaag atttgacgat
    49381 ccggatcttg tcttctggcg ttacccgagc atagacagag agagctttga cacgcgcatc
    49441 taattcttcg tctgataatt gatgcagttc tgatcctgaa agtgcttctg acggatcatt
    49501 caagatacct aattctttcg ctatggcact ggcagtcacc acatgatctc cagtgatcat
    49561 gactgttttg atgccagcct ttttcgctcg cttgattgct cctttacttt ctggacgtgg
    49621 tggatcgatc attccgatca gaccaatcaa acgtaagtct ttttccagtg cttcagaaga
    49681 aatatcttgc ggttcttcgt cataagtagc ataaccaaca gcaatcactc tcagtgcccg
    49741 tttaccaaag cgatcattaa cgatcgccgc ttgttctaca tcgcctgaac taaagcgcgg
    49801 agcaagaaca tcgaatgctc ctttggtgat tgagatgtat ttttcgccca tttggtgaac
    49861 tgttgtcatc atttttcgtt cagaatcaaa aggaagctcg tttactcgtg gatatttttc
    49921 ttctaattct gcctttgtat gataattctc ttcaacagcc cttacgatgg ctacttctgt
    49981 tggattacct ttgataacaa ggtcgtcatc ttcttgctcg accgtcacat ctgtacatag
    50041 tgcagccatt ttcaatattt ccatcgcgct gttcgtcatt gaatcttcga tattcgttac
    50101 ttcatcttct ttgctccata cccggcggac gcgcatttca ttttgtgtca gcgttcctgt
    50161 tttgtctgag cagataacat tagcggttcc tagcgtttca actgctggca actgtcggat
    50221 gatcgcatgt ttcctcgcca tcttctgcac accgtatgca agtgtcaacg taacaataac
    50281 agtcaaggtt tccggtacag ctgcaacagc caaggaaatc gaagtcatga acatctcaag
    50341 gagtggttct ccttgcagtt caccaatcac aaatactaaa gccgcagccg ctaaagcgat
    50401 catactgatt cgtttgccta gctgattcaa tcggacctgt aaaggtgttt tttgcatagt
    50461 attttcgttc aaaagatccg cgatcttacc catttctgta gccatgccaa tggcagtgac
    50521 gatcgctttc ccacgacctg cagcaaccgt acatccttta aataccatat taagttggtc
    50581 acctaatgac tcatcttctt cagcaataaa tgctgcatct ttttcaactg cttcactttc
    50641 tccagtcaac gccgattctt ctacacgtaa ttgtgatgct tcgatcaaac gggcatcggc
    50701 agaaatagca tcaccggact ctaaaacaag aatatcaccc actaccagtt gctctgactc
    50761 aacagtggtc actttgccgt ctcgtatgac agttgccgtt tgtttattca tatcttgcaa
    50821 ggattccagt gctttttctg cgtttccttc ttggacaatc gccaataccg agttgatcac
    50881 aacgatagcg atgatcagca gtccttcaaa taaatcccca tgttctgtag caaatgcggt
    50941 ataaaaggaa atcaccgctg ctacaagaag gatgatcgtt gtaaaatcgg acaagcttcg
    51001 aaagaatttt ttgatcatcg attctttcgg cgcctcatcg aatttattca atccctgctt
    51061 ttttcgccgt tcatccactt cttgtgaaga caacccagta tctggatctg cttctgttac
    51121 cttctttact tcttctattg tctgcttgta ttcttccatc ctattcctcc tttaaaatct
    51181 tcgtacaaaa aaaggtactt attttactat aacatccttt ttttatttac ttcaaataaa
    51241 gggcttatcg attatttgtt aagaaagcgt aaaatttaaa gtgcttatat ttgcaaacgc
    51301 taccaaagga tgctattata tagatgtaag gaaggaggat accatcaaat agacagaaga
    51361 atttctcgat agaacagtgt aagcaaacaa agttgttaga agtacaggtt tcacccagac
    51421 atgcgtgtag agtacaaacc tataaatagt gaatgcgtat ggatgtttgg atcacgaagt
    51481 atccgaaaca agaagtaatg cgatacgtaa caattgaata acgtaaacgt ctgaaaattg
    51541 aataggaaca agagatgttt caaatgttga agatgtaacg aaaacaaaat ttttgtttaa
    51601 catgaagaaa ttcaacaccg tgctttgcat gtatgatgga aagttacaat catttctgtt
    51661 tatttgaatc cttccgaggt acaagaaagc cgagcttatc gagagctcgg ctttttcttt
    51721 gtcataaaat ctttgtaaag cttttttgtt attcaaaact agcggctttt cataacctta
    51781 tttcacgaac atcgcatctc caaaactaaa gaaacgatag cgctcttcaa tcgcatgctg
    51841 gtaagcagat aaagtcaatt ctcttcctgc aaaggcacta actaacatga ctaaagtcga
    51901 ttttggcaaa tggaaattag ttgagaaagc ttctaccact ttgaattcat agccaggcgt
    51961 aatgaaaata tcagtccatc cgctgtctgc tttgatttct ccattaaatt tcgttccaat
    52021 cgtttctaat gttcgaattg aagttgttcc gacagccacg attttcccgc catttttacg
    52081 tacttggttc aatcgctcag ccgcttcttc cgtcaaacgg tagaattcac tatgcatatg
    52141 gtgttcttca attttatcta cgctgacagg tcggaatgta cctaacccta catgtagtgt
    52201 cagatagact aattcaacac cctttgcttc gatctcatca agtaattctt tcgtaaaatg
    52261 caatccagct gttggcgctg cagcagaacc attttctttc gcatagacag tttgatagcg
    52321 atctgggtct tccagacgtt ctttgatata tggaggcaat ggcatttctc ccaatgattc
    52381 aagaatctct agaaaaattc cttcatactc aaagtcaatg atccgtccgc cgtgttccaa
    52441 ttcttctttc actactgctt gcaatctgcc atcaccaaaa gagattcttg tcccaacttt
    52501 ggcacgtttc gcaggtttga tcaatgtctc ccacgtatct ccttgtgtat tcgttagaag
    52561 aaggacttct aaatgcgcac cagtatctgg tttttctcca tataaacgag caggcagaac
    52621 tcgtgtatta ttcataacta gcgcatcacc aggctgtaac tcttcaatga tttcatggaa
    52681 atgtttatct tccatctctc cagttttccg atccaaaaca agtaaacgag aatgatcgcg
    52741 gtcttttaat ggtgtttgtg cgattaattc ttccggcaag tcaaaatcaa aatcttctgt
    52801 tgttaacata tcgtcactca ctttctatca tgtgcttcat cattctatca tgtttttcgt
    52861 tctgttggca aaatcagcac tttttcactt ctattttttg ttttcctaaa taaaaaaact
    52921 accctatttg tattttttca ttgttttagg catactagta atagatagtt cttcattgaa
    52981 aatagaaaaa gaggtggcac catgattgaa tttcaaaatg tgtcaaaaat ttataaaggt
    53041 ggaaaagtcg ctgtagagga cgtcaatctt tcatttgaaa agggtgagtt catttgtttt
    53101 atcggtacga gcggaagcgg aaaaacgacg acgatgcgga tgatcaaccg aatgactgat
    53161 ccaacacaag ggaaaatttt gattgatgga aaatccatcc aagaaattga tccagtggaa
    53221 ttgcgacgtc agatcggcta tgttatccaa agtattggat tgatgcctca catgacgatc
    53281 cgtgaaaata tcactcttgt ccaaaaattg ttgaaagtca gtcaagaaga acggaataaa
    53341 acagctgaaa aaatgattga tcttgtggaa ttgccccgtg agatgttaga tcgctaccca
    53401 catgaattat ctggtggtca gcagcagcgt attggagtcg ttcgtgccct tgcagcgaat
    53461 caagatatca tcttgatgga cgagccattc ggtgcgcttg atccaatcac tcgtgattcc
    53521 ttgcaagacc ttgtcaaaga tttacaagag cgtttgggaa aaacgatcgt atttgtaacc
    53581 catgacatgg atgaagcgat taaactagca agccgtatcg ccatcatgag tgaaggccgt
    53641 gtgatccaat ttgatacacc agaaaatatt ttacgtcatc ctgcaaacga tttcgtagaa
    53701 gagttgattg gagaagatcg cctactacaa gccaagccaa atgcgacaag agtcggcgaa
    53761 gttatgctaa atacagcaat cacgattaca ccggaaaaat cattacaaga agcaatcaaa
    53821 ctgatgaggg aaaaacgtgt agatacgttg ttagttaccg ataattcgaa tgtattaaaa
    53881 ggatttatcg atgtagaaac gttgaataaa aaacgaggaa aagtatccag tgtcggcgac
    53941 attttgaata aacatgtttt ttacgtaaaa gaatccgcct atcttcgtga taccttgcaa
    54001 cgcattttaa aacgcgggct gaaatacgtg ccggtcgttg atgaacagaa caaagtcgta
    54061 ggtatcctga ctcgtgcatc attagtggat atcgtctacg atgtgatttg gggagaagat
    54121 cccgcaacta ctgatgttgc agagtcagct actcctgaag aatctaagga ggtctgacct
    54181 atgcagcagt tttttcatca gcacggttca gaaattctct caaaaggttt tgaacatctt
    54241 tatatttcag gacttgcttt gatcattggt atcatcattg ctgtaccgct tggtgtctta
    54301 ctgacacgtt ttccaaaagc tgccacgatt gtgattggct taaccagtgc cttgcagaca
    54361 atcccttctc ttgctttatt ggcattgatg atcccgctat ttggcgtagg taaattacct
    54421 gcgattatcg cactctttat ttattcatta ttgccgattt tacgtaatac gtacattggg
    54481 atgaaaaatg tcgattggaa ttatcgggat gtggcaaaag gcatggggat gactgagttc
    54541 caatccattt tttccgttga attgcccatt gctatgccaa cgatcatggc tggtattcgt
    54601 ttagctgccg tttacgtgat tgcatgggct acacttgcct cttatatcgg cgctggtggt
    54661 ttaggcgatt tgatttttag tggactgaat aattaccaac cagatctcat tttggcaggt
    54721 acgatccctg taacaatttt agcactattg gctgattggc ttctaggtct cttggaacac
    54781 cggctgacac cgatcgcact tagagaggag aatgaagaat gaaacgtata ctaaaaatga
    54841 ccatggttct tttctctctt cttatacttg cgagttgttc cttccctggt cttgccagca
    54901 atacagatga agacaccatt tcgatcacag gtggaatcac ttcagaagca cagatcttag
    54961 caagtctagt tgccggaatg gttgaacatt atacagataa aaataccgca atcatcaaca
    55021 atctggctac tacaactatc aaccatcaag cgatgatgaa cggagatgct tcaatttcag
    55081 cggctcgcta cacaggaaca gatttaacga ctacattgaa ccttcctcct gaaaaagatc
    55141 cgaaaaaagc gtttgctacc gtgaaagatg aatttgagaa aagatacggt caaacgtggt
    55201 tcccgtccta tagttttgaa aatacgtatg tctttcttgt tcgaaaagac accgcacaaa
    55261 aataccatct atctaaagtc agcgatctga aaaatgtagc ggatgaatta gtagctggtg
    55321 tagatacttc ttggatcaat cgtaaaggag acggttatga cggcttccaa gaaacttatg
    55381 gcttctcctt caattcgata ttacctatgc agattggact tgtttatgac gcagtcgaag
    55441 caggaaaaat ggatattgtt cttggctact ctacggatgg acggatcgcc agttatgatc
    55501 tagtcatgtt ggaagatgat cgtcaattct tcccaccata tgatgctgct ccagttgtag
    55561 acaacaagat attagctgat acgcctcatt taaaagaagc cttaaataag ttaggtcata
    55621 ccatcagtac ggaaaaaatg caggaattga actacgaagc agataataat ctagtggaac
    55681 catcagtagt agcacaacgt ttcttacaag aaaaccacta ttttgaaggg aagtgaagca
    55741 attggataaa atgaatgtct ttcaacaatt tttctattat ttccaggaaa atggctccta
    55801 tatctttgca caatttatcc gtcacttcct gatttctatc tatggtgtcc tctttgcagc
    55861 agttgtcggg attccggttg ggatcatgat ttctaggaga cgtaaattag caaactgggt
    55921 gattcgtttg gcgaatatca tccagacgat cccttctcta gccatgatct ccattttaat
    55981 cattggatta ggcctgggag tcaatgtcgt gattgttact gtatttttgt attccctctt
    56041 accgatcatc aagaatacgt ataccgggat gatccaagta gacaaaaaca tccttgatgt
    56101 cggcaaaggc atggggatga ctgctagaca acgactattc atggtagagt taccactctc
    56161 tgtttctgtt atcatggcag gcataagaaa cgcgttagtt gttgccatcg ggataaccgc
    56221 gatcggtgca ttcgtcggtg ctggtggtct tggagatatc attatccgag gaaccaatgc
    56281 aacagagggc acatcgatta ttttagcagg tgcattgcca actgcattaa tggcgatcat
    56341 cacggactgg cttttaggta ttttggaaag acggctagat ccagccagcc gtacatcacg
    56401 ctgatgttat tgtaaaaaac aaaagcttct gcgaccattt gttggtcgca aaggcttttt
    56461 tagttctgtc atttttaagg caataatcca ctctgattct tccctcctta tgggcaaaga
    56521 gttgcttttc ttagctattt atccattccg tatactccaa cttcatactg ccaacgggtt
    56581 catatctgca tccaccgact aagttttttt cttatccgct aaggcatttt gccaatccat
    56641 tttcccatca ctcgtcatgg aattcatacc acatgatctc taaatactcg tctgcttgtt
    56701 ctttgaattg ggcatcaatt cttacatcct ttggcgtggc tttcatttaa gccttttcca
    56761 tcagtcctcg attgttcgat gccttcagct gcatcatcaa aactggtgac tgccgtatcg
    56821 gtaacttcga tcatctttta tacaatcggg aacttgccat cctcatcctt ggctaaaaac
    56881 atatctagcc attccagctt ctcgccatgg gaggtttgcg gttcctaaaa ccggtacacg
    56941 attcttatgg actgcatccg tcgcaactag acttggcgat acgatgatcc ttcaccagaa
    57001 gagcaccccc gtaaaccaat tgatcgatat attgccaata agaaaagaca ttggcgtaaa
    57061 agatatcgat ccctcgtgat gcattgccgt tgatactgct attcatggcc gaaagcgcca
    57121 cgactttaac ttcttccgtt tgagtgacat tcatgttcgg caatgtcgct ttggataccc
    57181 gctctgctaa aggaacgaca ctggtattgt atttcgtatc tgtatctttt ccaaaagacc
    57241 atgccaacga atcctctggg aaccaataag acgactccgg ttgattgtcc attgcttttt
    57301 tcaacatact tttggctgtt tctcgataaa cgacatttcc aattgtcttc gcctcacttt
    57361 ggcgataatc gatggtcgtt gccactgcca acactaacaa cgatgctcca attttactga
    57421 ttttccttgt cacacttctt cctccttgtt ttacataaac ccgtcaacat tccattccta
    57481 aactcaaaca atgacccttc aaaggtggag atataggaac aaattaaaga aatcatgcaa
    57541 taaatccaag aaggttggtt ttagaacaaa aaaagagtct cctttttggg gagactcttt
    57601 gttaactatc taaaaaggtt ggctcttctc acttcactca taatgtgtcc aggcagaata
    57661 aatttctaca actttatttt cctcatcaac tttgtaaacc actcgatgct gtctattcag
    57721 cctgcgtgag tatctgcctt catgtgtagg tctcagcttt tcaaaagact gcgtgggcat
    57781 atatggatct tccttcaatg tctgaatcac ttcttcaaat tgtttcttta aattcgtttg
    57841 cttgattttt cttaaatcca ctttcgcaga gtttttgatt gcgactgtat agttactcat
    57901 caaagagctt cccaatcaat atcatcgata ttcgtaaagc tactgtcgtc tttttcgcgg
    57961 tctcgtactt tatctaaagt acctgtttgc tcaagaaaaa gggtttcttg aatcgctctc
    58021 cagtcttcca gcccaatcaa tacagcattg tttccactac gatcactaat aatctcgatt
    58081 tcagtatgat tttcatttac ctctttcaat aactgataaa aatttttacg agcagtgcta
    58141 ggattcatcg ttgtattagc cattgtctct cctccttttt ctgatataat tatagcgtac
    58201 ttgattaagt acgtcaaaaa aatacaataa agcacttaaa ttcagcaaac ttttcctctt
    58261 ttttacatag agaaaaagac ccctttttag ggaggatctt tgtccacgct cacttcttac
    58321 tcctttacga ctagcggtag ccaaacgtaa agtaattggt aacaacgtat catctttagc
    58381 aaaggtcgaa tccaaagcct gtgagtctat gcaacaacac caatacacga aggttcctcg
    58441 ttgcagtgtg acttgattga tatcttgaat aacctgagga tacgcacgaa agatttgtaa
    58501 cggttattca aaactgattt tggccgtcaa gtcttctact gcctcaaaaa tgattgcctc
    58561 tttcgtctcc tgtcattcat gttcactatc gatttgatac ttgcttagac cgttgggttt
    58621 gtgaatcacc aattaaaaat ggcactgagt tatggtaaca aaaaatggtc acttgacgag
    58681 gctgagattc ctacttatga agacacatgg cacttaagag tcgaggtaaa ggaagaagct
    58741 gaaaatatag gaggctgtac attatttggc aaaggattcg gaaattatga tcaagacatc
    58801 aaaatcaaaa tgttttacag ctaaatagga agaataataa catttcatac gctgttccca
    58861 tctttaatgg cttatagagg gcacctttag ctgagaataa gacaaaacag accctctagc
    58921 ttcaattaag agctagaggg tctgtttttg attttaaaac ttggtttatt tcttcaaatg
    58981 gtagctatag gtgctgacaa cgatattttc acgccaagac aatcgcaatc ttaaccgcaa
    59041 acttgtttgg ttatggagaa tattctgcca gcgtctgttt ggtacaaatt gaggaatcaa
    59101 cacagtagtt gtatagtttt gtttcgctgc atttttactt acgatatcta cataacggat
    59161 aattggattt tcaatcgagc gataagaaga gtggacgatt gaaaaacgga catctgggaa
    59221 atgcttttta aattctgctt cgatttcttt ttctttttcg acatcttcgt ctagcgaaac
    59281 atgcatcgca acgacataat caccgatcga acgtgcgtaa ttcaacgcac cgatatttac
    59341 ccgtgtcaca ttcccgacta atacaagaac ggtattcccg tcataatcat gtagttttgc
    59401 atcattttct aaacgaagct gttctgctac ttttttataa tgatcatgga tcttataaaa
    59461 aatgaacata acgattggca taatgatgaa gaaaggccaa atgtccccta accgatagac
    59521 aaacaaaatc gcaatgattg catatgagat gaacgcaccc gtaatattgg caatcgattt
    59581 gcttaaccat ctttttcctt cttttttcca tttgacgacc atccctgttt gagataaagc
    59641 aaatggaatg aacacgccga tagagtataa cggaatcaag cgttctgttg agccatggaa
    59701 aataaacagc aatacgatcg aacctagcgc tagtgtgata atcccattgg aatagcctag
    59761 tcgatcccca cgatcttgat acatgtgagg catgaattta tcttttgcca gattgtatgc
    59821 aagaactgga aaagcagaaa aaccggtgtt agcagcaact gctaaaatca gcgctgtagc
    59881 aaattgaagg acgtaataca aaataccatg tccaaagaca gctttaccaa tttgagacaa
    59941 aacagtgact tctttttcag gaacgatccc ataccaatag ttgataaatg tgatccccac
    60001 gaagaagaaa cctaagatca atgccatcat cgtcaatgta gcagcggcat tttttgctct
    60061 tggcttctta aagaatggta cagcattact aatcgcctcc accccagtta aagaggaaga
    60121 accagaagaa aatgctcgta aaatcaaagc gatagaaaca cccggcacag ccgctcctgg
    60181 aagtgcagta gcttgcaatg gcacagcacc tgtcacgata ttatataaac caacaacgat
    60241 cagtaacgag atcacaagga taaatgtgta caccggaaat aataggaacg atgcagattc
    60301 cctcagccca cgaagattca gcatcatcag tagcaaaacg attgtcacag aaatagcgac
    60361 ttgatgaccg tagagtgctg gcaccgctga tgtgatcgct tcagcacctg ctgagacaga
    60421 tactgctact gtcaacatgt agtcgatcaa taaagagcca ccggaaatca agccagcatt
    60481 ctttccaaga ttctcactgc tgacgacata tgctcccccg ccatgaggat aagcatggat
    60541 gatttgacga tatgatagcg ttaatgaaat caataaaata ataacaaaag cggcaatggg
    60601 tagcgaatac cagatggctg cagcagacaa agcgactaaa acgacaacga tttgttccgt
    60661 accatatgca atagatgata acgcatcaga cgacaataat gccaaagcag caaatcgggt
    60721 taatttgtgc tcatcatttt cagcagattt taaaggctta cctactaaca acctttttaa
    60781 gtaatccaat ttctaaactc ctttacacac aattttactt caagttttat agtcgaaaat
    60841 ttatagtaaa caagatgtta tagtacgctc actgacctta aaagtcaata aactttaaga
    60901 aaataattgg aaaaaaacag aaaaatatca agcgcttttt caaacgcttt aatacagcaa
    60961 aaatctaaca tttttttatg ggctttttct ttgaattcta taataaaagg atagaacttg
    61021 acttagaaaa tcaagttcta tcctttttga tatcttaact tttggaagag agatgtaagg
    61081 ctgctttcta cccatcattc ttactactcg aagcatagcg cagttgtcac aacctcttga
    61141 taaacggtgt ttaaaagctt gcatctgcgg taataagccc aaccaaatta aaaacataag
    61201 aagctgtttt tatttggtcg ttcttattac ttgatgcaga gcagcttttt tacaacctct
    61261 tggtacacgg tgttcaatca gctgaccctc ggtattagct caaaaaaacg gcaaaatatg
    61321 aggaactatt tttccatttt tttctctaat actcaggtca aaacactgat ttcacaacct
    61381 ctttttttaa tgaaaaatat ggctctcatg cgccgcttct ttgaatttcg tcattttttc
    61441 ttcgatgaac ggatagattg ccgttcctac gattccaaag acaacaaaca aaccgatcat
    61501 gatccagatt gctttcgtag catttggcca ataaattcct cctgcagatt cccgtaacaa
    61561 gtttaccgca tgagtaaatg gcaagaacgg attgatcatt tggaagaatt tccctgagac
    61621 ttggatcgga tagttcccgc ctccgccaga gatcgacagt actaagatga taatagcgat
    61681 ccctttaccg acattatcga ataatgcagc caagacatag acgatcatca tgaacgctaa
    61741 ggcgatcaag acagcaaatg acacgctgta gaccggattt tggacatcta ctcctagtaa
    61801 gaaaatattc cctaatgtaa cgatcaagct ttgcgcaatc gccattgtca aaaatgtcag
    61861 catacgagca acgaattgtt ctcgtttcgt aaaattgttt cggtctttct ttcctaaata
    61921 gtattctgtc gtagcaacac ttgaaagaag aacagcgccg acccacagac atagtgcagt
    61981 ataaaacggg gtgctagctg aaccgttatt tgcgattgga taaagcgcat tggtttgtag
    62041 ctcaacaggt tgtgtaaaga agtcactttc ttttgttgcg tctagtttca ataatttgat
    62101 gacttcgcct agatcgactt cttgttcgcc ttttcggatc gcgtctgctg ctttttggat
    62161 accagctttg atatttggcc agtcattgtt gatcaagtcc gcagcagcgt tcacagctgt
    62221 ttcgacttct ggcatttttt catttactgt tgtcaatgta ccggtgattt cattttttag
    62281 tgctggccaa tcattttgga taaaatcagc ggccaatcct aatttttgct gaatgatcgg
    62341 taattcattg ttgtataggt ctgccccttc attgatgcca ttgacgatgg tatccatatt
    62401 tccattcagc attgtattgg catcatggat ttcttgtcca attgcaggca tttctttttg
    62461 atatttttct aaaattgcca ctgcattcgt tacagttgct ttcgttgagc tgagaagcga
    62521 atcaaagtcg atctggtccg ctttgctcag tgcgccttgt gcagtcccaa tcgtatcgat
    62581 gacttggtct agtagagaag tgacagtgga actgatttga cctgtattga taccagcaat
    62641 gatgccattg atattattaa caacggcatc aatttgatct agcaattcat caatagcctt
    62701 cgtgtcttca ttttgaacag ctgtgttcaa agcagatagg cggttttgca gattgcttag
    62761 caagggtttt aattgattta gctgatcaat aattggctgt aatttcgtat ttccagcact
    62821 tgtttgcaat ttagtcagga actcgaccag tttatcgatt gcattttgtt gttgagatat
    62881 tccgtcaata aagccgttca atacattgct gatgtttttc ttttcatcat cagtcagctt
    62941 accatcttca atagctgttc ttacagtatc tattaacgaa cctgtcgttg ttgccacttg
    63001 tccaattgac ttcaatgtaa catctacact gtttgtgatg cttggtatcg catctttcag
    63061 ctgagaggca cctgattttg tttggtttgc tagatcactt gcctgatttt ctaaattttt
    63121 cacatctggc aagatatctt gtacttgttg gatgatcgtc aagccttgtt ttgcttcatc
    63181 gattccttgg ttcattgtct cttggatcga agcaaaatcg ccgtcgatct ctgctagttg
    63241 cttaccggca ttttggatct ctgggatttt ttcttgcaaa tcaaggatga tttttgcttg
    63301 atctttcaac tcaggcattt tgtcattcaa tgcaacgact ttttcaccca tctcatctac
    63361 tttagggatg taatccacga attcatttgc tttattcagt ttttccttga tttcaggtag
    63421 ttgactttgt aattcgagga cttgttttgt gtagccgtcg atcgtgtcca aattttcatc
    63481 tgttgataaa atcatatctt taactttatt gatacttact aggttcgtat cgatgtcgta
    63541 acctatctca ttgaatactt tcaataaagt cgaactggct gttttgataa attcatttgt
    63601 gatttgcgat tggatcgaac ttgctccttt atcggtgatt ttcggtgcaa tcgcattgat
    63661 tttttcattt accgtatatt cgatttttgg tttcttgata tcaccactag taaaactcag
    63721 cagatcttct gagaaatcct ttggcaagta aatacctgcg taatactttc cggaacggac
    63781 tccgtcttct aattgttctt ttgaatcaac gaattgccat cctaattgtt tatttttatg
    63841 aagcgattcg atgacttgct ccccaatggc cacttcttta ccttgaaact ctgctggctt
    63901 gtctgcactg taaacagcaa ttggcagctc tccagtattt ccatatggat cccataatgc
    63961 tttgatgtta aaccacgcat atagcgaagg gatgatcatg atcgcgatga tcagaagtgt
    64021 tgcaatcggg tttttgaaaa tccgtttcca gtcaagcaaa tataatcgaa gcgtattttt
    64081 gattgactgc atagttctcc cccttcttct tttttcagct gaattacttt accatgtatt
    64141 tcatactttt ttcaatcggc ttttttcaaa aaagtcgaat ttttggacat atcacggcaa
    64201 ttgtctcatt gtaatctctt acatattgag aaataattaa ggattacagc ctttacttta
    64261 tcatgaaata aaagggaaag ataattttat gccaaagttt ttatctatta tacgtatacg
    64321 caaaaaagct aaaacatttt ttgtctttgc ccctacaaaa atgtttcagc ttttttcgtt
    64381 ttttattcct gtatttctta tttttcgaat gacaggatga ttttcccagc aggaggatgt
    64441 ccttcaacta gctgatgtgc ttggatgaca tgcttaagtg aagcagacag attcattgaa
    64501 ataaaaacat gaaaggctcc tttttggtag gctttgatta tgccagcaaa ggcttctgca
    64561 tctagatatt cctttcttgg aacaaacgat tcataaaagc cttccttttt ttgccgcaaa
    64621 tctagatctg ggaggtcatt taatgcaaca tacctgccgc ctggtttcat gatctggatg
    64681 ccggtttctc ctttgatact tcctttcgtc gcatcgatta caaggtctgc ctgatcagca
    64741 aattgcagac ctggatttgt tttatcataa gcagcaaatg ctgaagctcc taattttttt
    64801 accttttcct catttttact ggatgcactg gttaagattc ggattccctt ttcatggaga
    64861 agctggatca gacttgaccc gaccgcacct gatgctccta gtatcattac agtatctgtc
    64921 ggctggattt ccgtcaaatg gttgattaga ttataagccg taattcctgg cgtgaccatt
    64981 ccggctgctt cttcccaact cattttatct ggaatttttg ctacttttgc tgatggcaac
    65041 acgacttctt ctccgtaagt cccgccgact gcatggacag ctactcgatc acctacatga
    65101 acagttgtga catctgaacc aatctctgtc acaataccag cgccatcatt acctggtaca
    65161 taagggaatt tcaatgttcg tatctctttc atttctccta aacgcaaggc aacatcataa
    65221 ggattcacac tgaatgcttt tatttctaca cgaacatgtc cagaaccgac ttctctggga
    65281 tgagcatcga tttcttcaaa aacatcttgt gcttctccgt aaccgttaat tgcaaatctt
    65341 cgcatggtca ccatctccct ttttctttaa gtttacgttt atgcctttat tttgacaaaa
    65401 aaaatgtctt ttcgttttgg cgcatgattc aaaacaaaat agttgtcttt tttcgcgtaa
    65461 cgtgttaaat tatttatcta aaagcgtaaa gattacgatt tacgctaata aaaaataatt
    65521 ggaggaattt gttgatggct aaaaaatcaa agattgccaa agcaaaaaaa cagcaagaaa
    65581 cgattgaacg ctatgctgct ttacgccagg aattaaaagc gcaaaaagat tacgaaggct
    65641 tgcgtaaact tcctttagat gctcgtccag aacgattgaa aaatcgtgat ttaatcgatg
    65701 gacgccctcg cggatacatg cgtaaattcg ggatgtcccg tataaaattc cgtgaattag
    65761 cacataaagg tttgattcca ggagtaaaaa aagccagctg gtaatccaag tctggctgta
    65821 atgtaaagga ggagggcgca tggaccactc cttggatcat caaaaaagat acgttcgctt
    65881 cttctagcag aaatgctggt actagaagca gacgtatctt tttttacgca cagctggtcc
    65941 ttttttatat gtaaaggctg catggttact tcgctatatt tatttagtaa caggagtcag
    66001 tgcttcgata cccacactaa ttttcaatgc tcggtcaact tctgccataa tgggtcgatc
    66061 caaatggcag accttttctt tcaatcttga tttatcgatc gttctgatct gctccaacag
    66121 gataacggag tctctttcta ttcctgttcc agtagctttg atcccaatgt gagtcggaag
    66181 cttaggttta gccatttttg ccgttatggc cgcaacaatg atcgtcgggc taaaatggtt
    66241 acctagatta ttttgaataa ccaaaactgg gcgtgttccg ccttgctccg atcccacgac
    66301 tggagaaaga tcagcaaaat aaatatctcc tctttttacc ataggatcct ccttattctt
    66361 gatattctct tggtatccgc tgtcctaatc cgcaagccac ttcatagtgg atcgtaccta
    66421 attgatctgc tatatcctgc atagtgattt ctttgtctgc atttttgccg ataagcgtca
    66481 cttttgttcc gacaggaaat tcttgaggca atcggatcat cagttgatcc atacatacac
    66541 gcccaatcgt ttcacagtag ttcccttcta ccaatagaga aaaaccctgc atctttcgtg
    66601 gccagccatc tgcataaccg atcggtatcg ttccgatcca ctctgcttca ggagtgatat
    66661 atgtttctcc atagccgatt ccttcacctg ctgggagttt tttgacttga atcaattcag
    66721 aaaccagctc aagtgctggc tgcaatggat aaacttccgg tagtgcgtgc ccagagggat
    66781 taagtccgta cattgctact ccataacgaa tcatattgcc gattgtttca tcatgccata
    66841 aagccgttgc actattgctg acatgtacat atcgaggaag ttcgggaagt tttttcagca
    66901 cttctataaa gcgttctttt tgcagattcc aatatgtatc atctgcctga tctgctgtag
    66961 aaaaatgggt aaataaacct tcccattcta atacacgact ttctttgatg aatgcagctg
    67021 cctcttttac ttcttcaggt gtacaaaatc cgatacgacc catcccagtg tctgctttta
    67081 gatgaatcga aagaggcgtc tctagcttac ttttctctaa aacagcagct gctgcttgca
    67141 accattcccg tgtacctgca gttactgata aatcatgagc aacagctaca gtcagtgcgt
    67201 cgtaaggaac catattcaag ataaggatgg gctgagtaaa tccagcttct ctcaactcga
    67261 ctccttcatc taaatttgaa acacaaaagc ccgaagctcc acctgcaact gctgcctcag
    67321 ctgtttcaat agctccatga ccgtatccgt tcgcttttac tactgcaaac aattcttttc
    67381 cttgcggaag tcttgcaact tcatttgcta cattttcagt tatagctttt ttatgtatca
    67441 ctgctttcgt tgggcggtgc caagcgacaa ccatcgtttt tccttctttc tagtcttcca
    67501 aaataatttg tgcaaaagct gtttcatttg tatgagtaat actgacccat acattgcctt
    67561 catgaggtga acgcgtcaca acaggctgac cattttcatt ttttaaaatc tcgacatctt
    67621 gaaaggtaac ttttccgatc ccagttcccc aagctttaga gaaagcctct ttgcaagcgt
    67681 aacgcccgcc taaaaattct acttggcgat gaaaaggcaa ggactggaat agtttcattt
    67741 catcagaggt caagattcgt gcaatgaatt ttggtttttc ttcgatcagc cgagtgatcc
    67801 taggaagttc gactgcatcg atcccaattc cttttatcat atgcttttct cttttcaata
    67861 aatttagaac actctcattc taccaaatta taaccatttt gccacaaata agtccacaaa
    67921 taacaaaaat gaaagtttct cttaaaaaag agaaactttc attttatgtt taatcgttat
    67981 ttgaacggat tacaaatccg cgttttttat cattgtgacg tttaccgcca tctttttggc
    68041 tgttattatt tttgctataa ccgccttttg atttgtttcc acgataatta ccgccgtcac
    68101 gacgattacg gttgtttccg ccaccacgtt tgccattttt attgtatcct tttttctgca
    68161 ttggcaatgg gcgttctggt gtgatcttca ctggcactgc atcagctgga tcttttgctg
    68221 tcgtttttag caaaagtgca actaaatctt gtgcagaata ttcttctaat aatttatctg
    68281 ctgtttgcag gtatttatct agaccatttt ctgcaagttt tgtttcgatt tgttcaactg
    68341 cagcacctaa ttgacctttg aatgcttctt tttcggttgg tggacgtaaa gtcgtcatcc
    68401 gttttttcgt caagttttca atcacatgca agtagtccat ttcatttggt gtaacgaaag
    68461 tcacagacat accgcctttt ccggcacggc ctgtacgtcc gatacggtga acgtagcttt
    68521 ctggatcttg agggatatca tagttgtaca catgagtgac acctgaaata tctaatccac
    68581 gtgcagcaac gtctgtcgct acaaggatat ctaaatgacc gctcttaaat gaacgcaata
    68641 cgctcattcg tttttgttga gacaaatcgc catggattcc ttctgcacgg taaccgcgtg
    68701 cttctaatcc gcgtgccaat tcgtctacac ggcgttttgt acgtccgaag acgattgtta
    68761 attcaggtgt ttgaacatca aacaagcgag tcatgatatc gaatttttcg tattcttttg
    68821 cgcggacata gtattgatcg atcaagtcag ctgtcatttc tttcgctttg atcttcacat
    68881 gatgtggatt tttcatgaat ttaacgccaa tattcttgat agctggcggc atcgttgctg
    68941 agaaaagtaa tgtttgtctt tgatcaggaa cttgtgaaat gattttttcg atatcttcca
    69001 agaagcccat attcaacatt tcatctgctt catccaaaac taatgtttga acggttccaa
    69061 gtttcaatgt gtggcggtta atgtggtcaa gcatacgtcc tggtgtaccc acaacgatat
    69121 gtggacgatc tttcaagcca cggatctgac ggccaatatc tgctccgccg taaacggctt
    69181 gtacacgtac ttttttgtcg cgccctaaac gatataattc ttcttgtgtt tggatagcta
    69241 attcacgtgt tggtgcgata actaaacctt gtaattcgtg acgatctgga tcgatttttt
    69301 ctaacattgg taatccaaat gcagctgttt tccctgtacc tgtctgtgct tgtccaataa
    69361 cgtcttttcc agctaatgct agtggaatcg tttctgcttg gataggcgtt gcttcttcaa
    69421 aaccagcgcg ttcgactgat ttcaacaatt ctggtgataa ttctagttct ttaaatttca
    69481 aatgaatcct cctatatggt tgttaactga ggatgtagct tttttgctac cccgtcgatt
    69541 tgttcggtca aagtgtattg tttctttttg acagtggcca cgcacgtttt tatccattct
    69601 tagcccctgt ggcaccaccg aaaaatcact ttgcgaagtt taggcattag aaaagccaaa
    69661 cgggtcgcgt tcagctttat cattattatg acatctggct tcaacaagtt gtttctatgg
    69721 caaaaccagt ccaatttctc ttcatctttc ggcaataggt tattctaaca tagtttttcg
    69781 ttttctgcaa gtttacagat ttttatttca tttgctgaag ttgttctacc acttcaaata
    69841 agcccatgct gttgctgcct tttagcataa tactatctgt tgcttcaaga ttttgtttga
    69901 tagcatcaat caatttattc ttttcttttt caaaatagaa cacaggtagt tgggggtatt
    69961 tttggttcaa ttcttctttt agtgcatgca tttgttcccc ataaaggaaa atcatggcga
    70021 atttctcagg atcgatatgt tcactcattt gcgcatgcat atttaaagac tctttcccta
    70081 attccagcat atctgctaac acagcgattc tgcgtccttt tgttggcaga tttttaaaag
    70141 tatctaatac cagtcccatg gctgttggat ttgcattgta gacatcgctc aaaatagctg
    70201 caccatttcc tgccgttagc cattcggttc tgttttgagt gatagaaaca tgggcgagcc
    70261 cttggcgtat ctcaggtatg gacagtccaa agaagcgccc gatcgtatac gcaatcaaag
    70321 cattcgttac attataactt ccaggcagcg ggatcgtaaa tgtttctcct tctactacaa
    70381 aatcggtttg tgcttttcct tttgctgtca cttccgcatg aacatcgcca tttccaatac
    70441 cgaatgttgt gactgtttgt actaatgttg ccgttaaagg tgtcagcaac ggttcatctg
    70501 cgggcacaac gagtaatccg tctgatacaa gtccgtcagt gatttccatt ttggctttag
    70561 cgattccttc tcgcgaccca agattttcta tgtgtgcttc accgatcatt gtgattgctg
    70621 cagcttttgg ttgagccata agtgaaagtt ccgtgatttc attggcgtga tccatgccca
    70681 tttccaaaac gatcttttct gtatcttggg gcatgtgcaa aatcgtgtaa ggaagaccaa
    70741 tattattgtt gtaatttcct tgcgtcttat atgttttgta tttttcagac aagacagacg
    70801 cggttagatc tttcgtggtg gttttaccat tgcttcctgt aattgcaacg acttctgcgc
    70861 ctactttttc caagtaatag gttgccaatt gctgcatggc tttcaaagga tctgctacag
    70921 gaataactgg aaaatttttt ggagcttgtt caacaggcaa gctccaaaat gaagcagatg
    70981 ctccgtttgt gatcgcttgc tggataaatt catgtccatc tcgcgtacca gcaagaggaa
    71041 cgaataaact tccttcggtt atcttccggc tgtcaaattc gacattggta atctttgtca
    71101 gtgcagagca ttcgctgccc acggcttgtg caatttcttg tattgttaga ttcattgaga
    71161 tctctcctaa aaaaggttgt gaaaaaagcg tttggcctaa acggcgggtt cgcatgactc
    71221 tcacttccaa gtaaatttct ctattcgtat ttagaagcta gaatgaacgc ggttacgcaa
    71281 tcactccagc ctcttcttta tttctcttta ttcatttttt tcaaaaaaag attgacgctg
    71341 atggtatcga ttcattccta actggatcag ttcttcaatc aaatcaccgt atttcaagcc
    71401 catattttcc cataagagtg ggtacatact gaactccgta aatcctggca tagagtttaa
    71461 ttcattcagg aataattcat ttttatttgt caaaaagaaa tcgcaccggc tcaatccgct
    71521 tccacctaac atcgtgtaag ctaacttcgc gtactcttgc gctttttgat aaacttcttc
    71581 tggcacttcg gctggaatct gcatttcgat tttattattg atatattttg cttcataatc
    71641 atagaatgct acgtctttta cgacttcacc aggcaaagtc gtccgaacgt cttcatttcc
    71701 taatacagca acttcgattt cgcgcgcttc aattccttgt tcaacgattg ctcgagaatc
    71761 atactgatag gctgttgcta aagcattttg cagctcttct cggttttctg cttttgtaat
    71821 gccgacacta gaacccatat tcgccggttt gacaaacatc ggataaagca aagaaccttc
    71881 acattgatca aatacttttt taggattttc tttccattga ttcttaagta ctggtacata
    71941 aggaacttgc ggcacaccag cagcttgtaa aatatacttg gtcatgattt tatccatggc
    72001 acatgcactg gtcaatacgc ctgcgccgac ataaggcata ttcaatgtct ctaagaagcc
    72061 ttggatcgtt ccatcttccc cgtttggccc atgtaaaact ggaaaaacga tggctccttc
    72121 ttctttgatt tcgcccggat tgatcacttt tcctgtaaag ccttcctctg tctgtccact
    72181 tgggtcccat gaaagatgca agacatcttt gctggcaggt ttttctgtta gtaatggacc
    72241 tttgacccat tgtccttctt ttgtaataaa tacgagttga acttggtaat aattataata
    72301 aatggcattt aaaactgaaa atgcggaaag aatggacact tcatgctctg cgctgcgtcc
    72361 gccatatagt aaagtaatct tcaaaagatt gtcctcctgt attctaaatc gttcagtgat
    72421 ttactctcat attttagcac aaaacaaaaa taaacgaata acttatttat aaaaaaacga
    72481 gccagcttct aaaagaacga acgtcattta ttttttgtca gctagattat ttgtcccgtt
    72541 ttttataaaa agttatgata aagaaaagga gggatgatga tggcgcaaac aaatccacgc
    72601 agccgcttgg cagaaatcat tcgagtgttc atcaattaca atgtagtgcc aaactttgta
    72661 cagcaaaaaa atcccgaaca ggtaaaaaaa gcctttgaag aactaggccc caccttcatc
    72721 aagataggac agatgctttc tgttcgagaa gatttgcttt cttctgcctt cactcaaaca
    72781 ttcaaaacat tacaagatag tgtacctagc gacacatttt caacggtcaa aaagacgatc
    72841 gaaacagaac tttctctttc attatcagat atattcgatg atttttcaaa atcgcctttt
    72901 gcctctgctt ctatggggca ggctcatcgt gccaagctga agaacggtga cgcggtagtc
    72961 gttaagatcc aacaccctaa catcgccgaa gagatccgac ttgatcttca actatttgaa
    73021 cgagcgatcc ctttgatcaa atacattcct gaaactagcg tagttgatct aaaaggggta
    73081 ctccaagaag taaaacgttc cttgatcaat gaaatggact tcttaaaaga aagtcaaaat
    73141 ggcgaacaat tctatcaaaa gaacaatggc tggaaagaga tccgcagccc caagatttat
    73201 gacgcttttt gttcgaaaaa agtgatcgtg atggaagaaa tgtctggtaa aaacttgaat
    73261 catttaatga acgccgaaaa caaaactgaa acttttatca ctggaattca aaacaagcag
    73321 ctgaaacaag aagttgcaaa gcttttagtt gagaacttta tgaaacaggt gtttgatgat
    73381 ggttttttcc atgctgatcc tcatccaggg aatctgctat tccatgtact aacaaaagaa
    73441 gaacagacac aagctagccg aaaaactgaa acagtccatg aaaaagagtt cggttccttt
    73501 gcttttcgag cctccacttc tgcagaagac cctgttgcgc catataccat caactacatc
    73561 gattttggta tgatggggca tctttctgca ggacttaggc aaaaactcac acaagccgtt
    73621 ttagcgctgt atacgaaaga tgcttatcga atcgaaaaag cggtccttcg actttgtcag
    73681 caagaaggct cgtttgatga aagtcggttc caccaagaac tgacaagttt tcttgaacag
    73741 tattatgatt ctcctattga tgaaatcaac ttgcaagaag tattcacaca tgtcgtgacg
    73801 atttgtcatc aaaataatct gcagttcgat cgtgatatca cgcttttgct gaaagcattt
    73861 gggacattag aaggggtgat ccgcgtcctc gatcctgaag tttccctcat ggaagtcgct
    73921 tcgccttttg ctcaacatta ttttctcact catctggatg tagaagatac attgaaacaa
    73981 agcggattag acttattgga agggatgaag gctgcgccta agatccctca acagctccac
    74041 catctattag aaatgtggac ttcgggacaa ggaaaagtca atttggaatt gaaaaagcaa
    74101 gacaaattat tgagcaggat cgaaagtatg atcaatcggc ttgttttcgg tatgatttta
    74161 gctgcattga tcgtagggtc ctccctgctt gtccaagcag cacctgtaga aaacgcagag
    74221 gtcgtttcct tattagggat atttacgtat gctattgccg cattcgtcat tatttttttg
    74281 gcaatcgatg cattgatcca aatgtataag aagaggaaaa agtagaaaaa ggttgtgaca
    74341 gaaagaatca gcttcaagaa ataagaaggg gttttacata aattgtttct caaatttttg
    74401 tgaaaattcg gcttattttt cgaagaggct acttctgaaa caccgagaat aaaaaatgtg
    74461 aaaaagctgt tctgcaccca gtaataagaa caatcaaaaa gggaacatgg aacacattta
    74521 tcctgtttcc ctgttccctt tttgatgtat tcgagcataa ataaaatacc ttcgttacct
    74581 actatcttct gtacttggta aaccttagtc aattgccttt tttaacgctt gacgaaaaga
    74641 catatgatct ttctccgtaa agggttttgg accctttgtc cttatgccag cttttcttaa
    74701 ctttccacta tgataacgtt gttctagaag cgtgccgata ttcgcttcgt caaattcttt
    74761 tccagagtga tggaacactc gcacaccctt cggcaataca agtgaagcta aaccataatc
    74821 ttgcgtgatc aaaaagtcat tcttcttaac cataccgacg attttataat ccgcactatc
    74881 agctccgcgg tctacgtaga cgatttggac atgattagcg tattctttgg tcgtataatg
    74941 gtccacactg gtcacgataa ccacgtcaag accataggat tctcctaatt ttatgacatc
    75001 ttcttttact ggagaaccat ctccatcaat gaatagcctc attctttttc gccttcttcc
    75061 ataaatgata aacagttact aatccgctca gtaaaagacc aacattgatc cattttgcca
    75121 agataagcag ttgtacactt tctatcggca ctgattcatg gttcagcgtg atcaataata
    75181 gatggattgg ctctcccatt tcttttgcta aaacaaacaa aagcgtcatc aataaaaact
    75241 ggtggatcac actcccttta ttttgagtag tatacttgac aacgatccgg cgatccattg
    75301 gaatagctgt atcagtttga taatattcct tatttagcca gctgcctgtt actacgtgcc
    75361 agaaactaag cagaaagaca cctatcgcaa aaccatttgg aatcaagagg accaataaag
    75421 aagaactcag tccaagcaat tgcatagcca atggagacca tgttaaacgt ttcaaaatcc
    75481 atggtccgac tatttttgct gcaatcaagc ctgctgcata aggcaaatac ataaaaatcg
    75541 aaagtttttc taatccgtac agtatgccaa cataaaaact gctaaataaa aaaaggaaat
    75601 tccctagcat tccatttaga aaagtaaaca gatttagcca gcttggcaac ttccattcct
    75661 tcttctggtg tcctaaaaca aacacgaaca agatgaacag cagcaagaaa ccataaatag
    75721 caagatcaaa aagcctttca tcgaataaca atcgggcatt tctcaaaaga aacaataagc
    75781 caaagaatat caaaaataaa acaagctctc gcgtagctac cagatttctt ttgatgtctt
    75841 tgaaatccag ttcgtaacgc ggataatgac taaccgtatg gtaggccatg acgtaaaaaa
    75901 gtgtataaat agtaaagctt gctgcaatct gagtcggtgc aggcaactca atcgcttgat
    75961 acaggagaaa tagccagata gctgcaaata agtacttttt cccatccata ttgataaaac
    76021 cttgcgtctt ttcgtgaaaa ttgaccgtgg tattagcagg tacaagccat gctgcactca
    76081 gccctagcaa aacacttgaa aaataaaata aaggaaaata aaatacgcct aatattccaa
    76141 gcaatgaacc agcaccacca atcagcagag cgatcatcag caacgtgtaa ctatctagac
    76201 caaatcgtag actgttgact aaaaatagtc cagtcatccg aaaggcataa aacagcgtaa
    76261 atggcaaaat cgtcagccaa ttatgcgagt ctgttagtcc caaaaataaa agataaggaa
    76321 taaaaataac cgtgttactt aagataaagg gcgttgtttg tgtaaaatta cgcccaaatc
    76381 gtttgagttt ttcgaacatg acaatggctc cttaaacagt cttataacta atctgctctt
    76441 cgattttagc attaaagggg aaataaacca attttacaga atatattctt gtaaaaaaaa
    76501 atacagaaag aagcataagc ttctttctgc atttttacgg tacttgatta ctcaagcaga
    76561 caagcccttc tccacaaaag tcttgcccga cacccctgca tccggcaggt gataaagata
    76621 gaaatcaatt atcctgtgcc tccaaaatca attaggagct ctgtaaacca gagtccttct
    76681 cgacccgtcg cataaattta ctttaacaca gttcctatgt ttatgcgaga gcttttttca
    76741 ttttataatc gaccatttga aatccttgac cgatatctga aataagttct tctgtaatct
    76801 caaatccttc atgcttataa acctcgattg cttgatgatt cccctgattc actcgcaaaa
    76861 agatctctct ttcctttgtt tgcgcagcaa gttcttcata ccagcgaaaa actgaagaag
    76921 tcaatccttg accacgtact ttatttagca aatataattt acttaaataa atttttccat
    76981 ttatctcttc ataagcagtg taaccgacag tcttttcttc gctttttaat aaaaagtagg
    77041 acacaccttc tgtcagttct ttttgaatct gtttttttga ctgataagtt ttcagcatgt
    77101 aatccacttg tttttgacca atgatcggca caaaaacttc cggccatact tcttgtatga
    77161 ctgccatcag ctcatccagt tcattttctg ttacttttat tttttccata cacccacgtc
    77221 cttttttaca ttataaatga ccttgcgctt gatggaataa aaagagcatg atcatgctat
    77281 acacacccac cgaccacggt ttcaagaaaa tcacaatctg cataaattcc ctaaaagata
    77341 atttcgtcaa accagctact agacagatca agtcgtctgg cgcaaaggga agcagcaagg
    77401 taacaactag tactttttta atatctttcg tttttcttgt cagcaattca tcaaatttct
    77461 gatacttttt tggcgataaa ataagctgga caaaggctac gccatagtaa cggacaagca
    77521 aaaatccgat tgcttcccca atcactaggc caatacaact atataaaagc ccaaatccat
    77581 tgccaaacat cagcatgccc gctactgagg agatccctcc cggcaaaata ggaatgatca
    77641 cttggatcac ttgcaacaga acaaatatca tcactgcata cttgccaaat tgctggataa
    77701 acgcttgcaa agacgtgctc gatcgaaaaa tcccatgatg ataagcataa agaatcaaca
    77761 caaagaaaaa aaacagacca ataatgggca tgatctgaag acatttttgt aaaactgatc
    77821 cttttttaga catctatatt ccccacatct tttttcattt attaaaactg cctcct
//
LOCUS       DACDYI010000002        76976 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.002, whole genome shotgun sequence.
ACCESSION   DACDYI010000002 DACDYI010000000
VERSION     DACDYI010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 76976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.002"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IWM52_000076"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IWM52_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP9972364.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="IWM52_000077"
     CDS             complement(322..891)
                     /locus_tag="IWM52_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAP9972365.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="IWM52_000078"
     CDS             1077..1979
                     /locus_tag="IWM52_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9972366.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IWM52_000079"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IWM52_000079"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAP9972367.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IWM52_000080"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IWM52_000080"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAP9972368.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IWM52_000081"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IWM52_000081"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAP9972369.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="IWM52_000082"
     CDS             8547..8732
                     /locus_tag="IWM52_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAP9972370.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="IWM52_000083"
     CDS             complement(8794..9990)
                     /locus_tag="IWM52_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9972371.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="IWM52_000084"
     CDS             complement(10160..11596)
                     /locus_tag="IWM52_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP9972372.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="IWM52_000085"
     CDS             11889..12698
                     /locus_tag="IWM52_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9972373.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            complement(12884..15037)
                     /locus_tag="IWM52_000086"
     CDS             complement(12884..15037)
                     /locus_tag="IWM52_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAP9972374.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(15331..16179)
                     /locus_tag="IWM52_000087"
     CDS             complement(15331..16179)
                     /locus_tag="IWM52_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9972375.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSLKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(16403..18343)
                     /gene="thrS"
                     /locus_tag="IWM52_000088"
     CDS             complement(16403..18343)
                     /gene="thrS"
                     /locus_tag="IWM52_000088"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAP9972376.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(18680..18859)
                     /locus_tag="IWM52_000089"
     CDS             complement(18680..18859)
                     /locus_tag="IWM52_000089"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAP9972377.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(18906..20729)
                     /gene="cadA"
                     /locus_tag="IWM52_000090"
     CDS             complement(18906..20729)
                     /gene="cadA"
                     /locus_tag="IWM52_000090"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP9972378.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            21007..23286
                     /locus_tag="IWM52_000091"
     CDS             21007..23286
                     /locus_tag="IWM52_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9972379.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(23379..24026)
                     /locus_tag="IWM52_000092"
     CDS             complement(23379..24026)
                     /locus_tag="IWM52_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972380.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(24205..24924)
                     /locus_tag="IWM52_000093"
     CDS             complement(24205..24924)
                     /locus_tag="IWM52_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9972381.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(25055..26206)
                     /locus_tag="IWM52_000094"
     CDS             complement(25055..26206)
                     /locus_tag="IWM52_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP9972382.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(26423..27163)
                     /locus_tag="IWM52_000095"
     CDS             complement(26423..27163)
                     /locus_tag="IWM52_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9972383.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(27167..27490)
                     /gene="rsfS"
                     /locus_tag="IWM52_000096"
     CDS             complement(27167..27490)
                     /gene="rsfS"
                     /locus_tag="IWM52_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAP9972384.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(27547..28140)
                     /locus_tag="IWM52_000097"
     CDS             complement(27547..28140)
                     /locus_tag="IWM52_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9972385.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(28124..28771)
                     /locus_tag="IWM52_000098"
     CDS             complement(28124..28771)
                     /locus_tag="IWM52_000098"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAP9972386.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(28784..29095)
                     /gene="yhbY"
                     /locus_tag="IWM52_000099"
     CDS             complement(28784..29095)
                     /gene="yhbY"
                     /locus_tag="IWM52_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAP9972387.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(29111..30220)
                     /gene="yqeH"
                     /locus_tag="IWM52_000100"
     CDS             complement(29111..30220)
                     /gene="yqeH"
                     /locus_tag="IWM52_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAP9972388.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(30217..30744)
                     /locus_tag="IWM52_000101"
     CDS             complement(30217..30744)
                     /locus_tag="IWM52_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAP9972389.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(30924..31709)
                     /locus_tag="IWM52_000102"
     CDS             complement(30924..31709)
                     /locus_tag="IWM52_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAP9972390.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(31702..32571)
                     /locus_tag="IWM52_000103"
     CDS             complement(31702..32571)
                     /locus_tag="IWM52_000103"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAP9972391.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(32572..33951)
                     /locus_tag="IWM52_000104"
     CDS             complement(32572..33951)
                     /locus_tag="IWM52_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAP9972392.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(33959..34378)
                     /gene="fabZ"
                     /locus_tag="IWM52_000105"
     CDS             complement(33959..34378)
                     /gene="fabZ"
                     /locus_tag="IWM52_000105"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP9972393.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(34403..34879)
                     /gene="accB"
                     /locus_tag="IWM52_000106"
     CDS             complement(34403..34879)
                     /gene="accB"
                     /locus_tag="IWM52_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAP9972394.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(34882..36117)
                     /gene="fabF"
                     /locus_tag="IWM52_000107"
     CDS             complement(34882..36117)
                     /gene="fabF"
                     /locus_tag="IWM52_000107"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAP9972395.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(36136..36873)
                     /gene="fabG"
                     /locus_tag="IWM52_000108"
     CDS             complement(36136..36873)
                     /gene="fabG"
                     /locus_tag="IWM52_000108"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAP9972396.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(36885..37802)
                     /gene="fabD"
                     /locus_tag="IWM52_000109"
     CDS             complement(36885..37802)
                     /gene="fabD"
                     /locus_tag="IWM52_000109"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP9972397.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(37909..38133)
                     /locus_tag="IWM52_000110"
     CDS             complement(37909..38133)
                     /locus_tag="IWM52_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP9972398.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(38186..39148)
                     /locus_tag="IWM52_000111"
     CDS             complement(38186..39148)
                     /locus_tag="IWM52_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAP9972399.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(39158..39598)
                     /locus_tag="IWM52_000112"
     CDS             complement(39158..39598)
                     /locus_tag="IWM52_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9972400.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            39904..40869
                     /locus_tag="IWM52_000113"
     CDS             39904..40869
                     /locus_tag="IWM52_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP9972401.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(40926..41156)
                     /locus_tag="IWM52_000114"
     CDS             complement(40926..41156)
                     /locus_tag="IWM52_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAP9972402.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(41242..42129)
                     /locus_tag="IWM52_000115"
     CDS             complement(41242..42129)
                     /locus_tag="IWM52_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP9972403.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTNTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(42218..43441)
                     /locus_tag="IWM52_000116"
     CDS             complement(42218..43441)
                     /locus_tag="IWM52_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9972404.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(43458..44537)
                     /locus_tag="IWM52_000117"
     CDS             complement(43458..44537)
                     /locus_tag="IWM52_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9972405.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(44668..46395)
                     /gene="ptsP"
                     /locus_tag="IWM52_000118"
     CDS             complement(44668..46395)
                     /gene="ptsP"
                     /locus_tag="IWM52_000118"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP9972406.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(46395..46661)
                     /locus_tag="IWM52_000119"
     CDS             complement(46395..46661)
                     /locus_tag="IWM52_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAP9972407.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            47140..49374
                     /locus_tag="IWM52_000120"
     CDS             47140..49374
                     /locus_tag="IWM52_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP9972408.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            49522..49842
                     /locus_tag="IWM52_000121"
     CDS             49522..49842
                     /locus_tag="IWM52_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP9972409.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(50047..50270)
                     /locus_tag="IWM52_000122"
                     /pseudo
     CDS             complement(50047..50270)
                     /locus_tag="IWM52_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(50468..52048)
                     /locus_tag="IWM52_000123"
     CDS             complement(50468..52048)
                     /locus_tag="IWM52_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAP9972410.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(52449..53825)
                     /locus_tag="IWM52_000124"
     CDS             complement(52449..53825)
                     /locus_tag="IWM52_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP9972411.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(53953..55071)
                     /locus_tag="IWM52_000125"
     CDS             complement(53953..55071)
                     /locus_tag="IWM52_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9972412.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            55213..55647
                     /locus_tag="IWM52_000126"
     CDS             55213..55647
                     /locus_tag="IWM52_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9972413.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(55725..56258)
                     /locus_tag="IWM52_000127"
     CDS             complement(55725..56258)
                     /locus_tag="IWM52_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAP9972414.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(56352..57530)
                     /gene="mutY"
                     /locus_tag="IWM52_000128"
     CDS             complement(56352..57530)
                     /gene="mutY"
                     /locus_tag="IWM52_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAP9972415.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(57523..58320)
                     /gene="recX"
                     /locus_tag="IWM52_000129"
     CDS             complement(57523..58320)
                     /gene="recX"
                     /locus_tag="IWM52_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAP9972416.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            58427..59809
                     /gene="rlmD"
                     /locus_tag="IWM52_000130"
     CDS             58427..59809
                     /gene="rlmD"
                     /locus_tag="IWM52_000130"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP9972417.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(59983..60561)
                     /locus_tag="IWM52_000131"
     CDS             complement(59983..60561)
                     /locus_tag="IWM52_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAP9972418.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            60883..61353
                     /locus_tag="IWM52_000132"
     CDS             60883..61353
                     /locus_tag="IWM52_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAP9972419.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(61461..62687)
                     /locus_tag="IWM52_000133"
     CDS             complement(61461..62687)
                     /locus_tag="IWM52_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAP9972420.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(63441..63641)
                     /locus_tag="IWM52_000134"
     CDS             complement(63441..63641)
                     /locus_tag="IWM52_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9972421.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(63775..64383)
                     /locus_tag="IWM52_000135"
     CDS             complement(63775..64383)
                     /locus_tag="IWM52_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAP9972422.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(64468..64938)
                     /locus_tag="IWM52_000136"
     CDS             complement(64468..64938)
                     /locus_tag="IWM52_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAP9972423.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(64986..65900)
                     /locus_tag="IWM52_000137"
     CDS             complement(64986..65900)
                     /locus_tag="IWM52_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP9972424.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            66046..66849
                     /locus_tag="IWM52_000138"
     CDS             66046..66849
                     /locus_tag="IWM52_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAP9972425.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            67027..67974
                     /locus_tag="IWM52_000139"
     CDS             67027..67974
                     /locus_tag="IWM52_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP9972426.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(68042..68335)
                     /locus_tag="IWM52_000140"
     CDS             complement(68042..68335)
                     /locus_tag="IWM52_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAP9972427.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(68363..69028)
                     /locus_tag="IWM52_000141"
     CDS             complement(68363..69028)
                     /locus_tag="IWM52_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAP9972428.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(69168..69800)
                     /locus_tag="IWM52_000142"
     CDS             complement(69168..69800)
                     /locus_tag="IWM52_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9972429.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(69807..70874)
                     /locus_tag="IWM52_000143"
     CDS             complement(69807..70874)
                     /locus_tag="IWM52_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9972430.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(70871..71602)
                     /locus_tag="IWM52_000144"
     CDS             complement(70871..71602)
                     /locus_tag="IWM52_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAP9972431.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(71771..72250)
                     /gene="greA"
                     /locus_tag="IWM52_000145"
     CDS             complement(71771..72250)
                     /gene="greA"
                     /locus_tag="IWM52_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAP9972432.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(72386..73561)
                     /gene="mltG"
                     /locus_tag="IWM52_000146"
     CDS             complement(72386..73561)
                     /gene="mltG"
                     /locus_tag="IWM52_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAP9972433.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            73765..74934
                     /locus_tag="IWM52_000147"
     CDS             73765..74934
                     /locus_tag="IWM52_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP9972434.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(75201..76823)
                     /locus_tag="IWM52_000148"
     CDS             complement(75201..76823)
                     /locus_tag="IWM52_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972435.1"
                     /translation="MNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAFHINR
                     ALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLYIVTL
                     ITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPLILLG
                     FIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIFLIIK
                     LIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLS
                     FGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLNIIQA
                     PWRFMIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNMVKEM
                     NFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPNKDEI
                     TFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTIRYSY
                     TFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT        22554 a        16044 c        13029 g        25349 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gacaattttt gtcacaacct tttttgccga gtaaacggtg ttcaaaagcc
    12781 agtttctcgg tatgaagtca aatttaagca aaaatgtgag aagctatttt cacaaaattt
    12841 ttcttcatac tcgaaactaa gcggcttttt cacgacctct tttttatttt gccatgaaca
    12901 tatcataata cgcgtccatt atttctttag caatcgtcaa gtttatgtgg tcttgttcgt
    12961 ctaataagtt cggcaagaca acactgatgg caatttccgg attgtctgtt ggtccgtatg
    13021 ccacgatatt actattgact gtcgcaatat ctggatgacc ttccgcaacc gtttctgcag
    13081 tccctgtttt tgcggaaagg tccattttcg cactggctaa tggtttggct gttgtgtaag
    13141 gatcggtacc atggacaact tggtggaatc cttcacggag gatcgccata ttttctgcag
    13201 aaatattgac cgtattttct actgttgtgc caatttcttt cttcacttca cccaagtttc
    13261 cattttcgtc attgccatag atcgctttga ctaggtgcgg ctgtactcgt ttcccgccat
    13321 tagcgactgt tgaagcatat tgagctaatt ggattggtgt atatgtgtca aactgtccaa
    13381 atgccaaatc ggtaaagtta cctggagcat accattcttt gccattatcc gcattgaact
    13441 tcttcatata atcaacagaa cgtgagatcc caggagattc attcggcaaa tcgatcccag
    13501 tcgttgtacc aagaccaaac tctttgaaag agtctctcaa ttgctgataa gctttagctt
    13561 cttcatctaa actaggtagt cccattccag gtgtatagtc tagtcctaac atctttaacg
    13621 ccactttgat catatacgta ttagaagaca attccgatgc tttgactgca tcaagagcaa
    13681 cttgtccgct tcgattaaag acagagcttt tttctgaagc accttgcaga cgaatcggct
    13741 catcaatcaa aacttgattt ccgctgatcg cgttacttgc ccagcctgct gtcaaagtac
    13801 cagccttgac tacagaccct ggatcaaagg cgctagtaat cgtgcctaaa gcattctcgg
    13861 tgatttcttt ggaaccttgt tcatgactga aaccagtcat tgctagcact tctcctgtct
    13921 gaggattcat agccactgca taagcaccag gcgaatactg tgctttgccg ttgttgatca
    13981 atgtttgata attccttttc aaaatttctt ctacttttga ttggaattct gcattcatag
    14041 ataacattaa attagaacct ttttccccag agaagatttc tttttgattc gaaacatttc
    14101 cttcattatc aagggaaact tcatattggg ttttggttcc ttgcaggaca tcttcatatt
    14161 gtttttccaa ataactttgt cctacacggt cattccgaga ataacctttt gctaggtatt
    14221 tctctgcttc atctgctgga agtccttgct tttcagtcgt cacactgcca agaatacttt
    14281 tcagtgaact tgctgcatta tattctcgtg tccagtctgt gccagtcgaa actcctggaa
    14341 gttcagaagc gcgttctgcc acaacagcta actcttgatc agtaacacca gagtttttga
    14401 tatagacagt gtttaattca tatgcactat tcatccgttt aaagatggta gcaattttta
    14461 attgctcctc gtttaaatta atctcatcag ctgtgacttt attgaccatc gcactatatt
    14521 gttcgctgtt ccctaattgc ttttcttttg ccgaaagtct ttttgtggct tctttcaaat
    14581 gtttgggatc agccagccaa aaatcttttt tatctctatc tgtcagattt tcatctaccg
    14641 gaacatcgat cagcgagttc agcttcgttg cgatttcaag caaatcttta gccgtaactt
    14701 gtgttcctct cgtatatgtg atggcctgat tcgctttatt cgtaactaaa gcttttcctg
    14761 tggagtcgta gatcattccg cgaggagtac tttcttggac cgtaatcgta gaagtagact
    14821 tcactcgcgc ctccatctct tcgccgttca cgatctgcaa ataacccaat ctagcaatca
    14881 acgcaacaaa taacgtaaat ataatgaaaa aaagaaaatt taagcgaaaa ggaacatggg
    14941 accggcgaaa cgttttgttc ttaggttctt gtgcaccttt tcttattttt tctaaccaag
    15001 aagtatttaa tatttttttc ataaaatctt tcatcatttc ctatccttcc gcctatttac
    15061 attcatgctt cattgtaccc aaaaacttct taataaaaaa gaaaaaatgg gcggagcaag
    15121 aaattcttta cctaatttca agataaaatc aactttttgt ttcacagcaa tcacttaaag
    15181 ttacatatcc aactattttt ttcattaaaa aataaactta tttttgaaaa ttatgttttc
    15241 ttaagtcgtg aaacactttg atggatcatt tatttgtgga aaactacgtt ttgttattct
    15301 aaaaaactta tataaaaaat agtaaggttt ttatttcgaa tccataaaat attcttggat
    15361 tgcttctcgt gtttttttca ctttttcgct aagcttttcg tttttagcca tatagaaata
    15421 aaaaatgata tcgatcgtag caaattgggc caaaagtgaa ttagttgccg cacttcgatt
    15481 cgtgatttct tttggtctag atgtttgtac gagtacatcg gctatcttgc taagcgggtt
    15541 atttccaaat tgcgtcaacg caatgatttc gatattcatg cttttcgcca gctcggctaa
    15601 tgcgactaca tctgcacttc tacctgagtt tgaaacaagc caaagcactt tctttttctc
    15661 attgctgaca agttgcggga gcaagacgtg gatatctttt tcaaaaatga ttggcttacc
    15721 gacacgagac catttttgca gaatatcttc tactactaaa gaagaggcgc caaccccata
    15781 taaaaaaaca gtctctgccg attccaaaca ctctacgact ttttggatcg tttcttcttc
    15841 tagaatatct actgtttctt ttatcgctac ttgtgcattg ttaccaagtt tgtttttgag
    15901 agagtaaaag gactcatttg cttctatatc actaaaacct tcatcgattc cagtctgtag
    15961 ttctgttgaa atcgtgattt tgaagtccgt cagactttga aaaccgattt ttttggaaaa
    16021 acgtacaact gtcggcgcac tcaacccgga agcttctgct aattcattag ctgtcattgt
    16081 agggaccttc tcaatattac cgataatgta gttggccaac tttttttctg ctgaagaata
    16141 atcttccatc atactttcaa tacgactaat tacgctcata agttcctcca cccttttaat
    16201 gatagtctat gtaagtatac catatcagac ccttattcac ggtcgtatac tggataaaaa
    16261 acaaaaaaac aatccgccca acagaatatc ttatcccatc aatcccacag attctattga
    16321 gcatctgtgt ctttgaggaa tacctgttat ctattgttcg gattgctttc tatctactac
    16381 ttttttacag gaaatttacc tattagtttt ctctgctgta atttttgact tctgcttcca
    16441 tagcatcaat aaagatattc aaatcttcta cagctgtttc tttacttcca taacgacgta
    16501 cgttcactgt tgcatcttcc aattccttgt ctccaacaac taattggtaa gggatctttt
    16561 gtgtttgaga ggcacgtatc ttatagccca ttttttcatt acgatcatca acttctacac
    16621 gcaacccctt catttgcaaa cgttctttga tttcataagc ataatcgcca tgtgcatcaa
    16681 cagaaacagg gatgattgtt gcttgtatag gtgccaacca tgttggaaaa gcgcctttgt
    16741 atacttctgt taaataagcg acaaaacgtt ccattgtaga aacaattccg cggtggataa
    16801 ctactggacg atgcgtattt tccccgtctt ctccaacata tgtcaaatca aagcgctctg
    16861 gcaataagaa gtctaattgt atagttgata atgtttcttc tgtaccaaga gctgttttga
    16921 cttgaacatc tagttttggt ccgtaaaagg ctgcctcgcc ttctgcttca aagtaatcta
    16981 gttctaattc atccatagcg gctttcagca ttgcttgggc tttttcccac attgcgtcat
    17041 catcaaaata tttatctgta ttgtttggat cgcgatagct caagcggaaa cgataatctg
    17101 taatattgaa gtcagcatat actgccacca tcaattcaag cgtacgttta aattcatctt
    17161 tgatttgatc aggacgaaca aatgtatgac catcattcaa cgtcatttct cgtacacgtt
    17221 gtaaacctga caaagcacct gatttttcat agcgatgcat cattcctaat tcagcgatac
    17281 gaattggtaa ttcacgataa gaatggatcg tgtttttgta gaccatcatg tgatgagggc
    17341 agttcattgg gcgaagaaca agcatttcgc catctcccat atccattggc gggaacatgt
    17401 cttcttgata atgatcccag tgtccagatg ttttgtacaa ctcaacatct cccattattg
    17461 gtgtatatac atgttggtaa cctagactaa cttctttgtc tacgatataa cgttcgatcg
    17521 tgcgtcggat tgttgcccct tttggaagcc agaatggcaa accagaacct acttctgggg
    17581 aaaccatgaa caagtcaagt tcttttccta gtttacgatg gtctctttct tttgcttctt
    17641 cacggagacg gatatattct ttcaaagctt tcttgtcaaa aaaggctgtc ccgtatacac
    17701 gttgcatcat ttgattgttt gagtttccac gccaataagc acctgccact gataataatt
    17761 taaatacttg tatacggcca gtggatggaa catgtggtcc acgacacaaa tcgatgaatt
    17821 cgccttgttg ataagcagta atcacttctt cttctggcaa ttcactaatc agttctactt
    17881 tgtacggatc acttgcaaaa atttctaatg cttcctgttt tgaaagaaca cggcgttcga
    17941 tcggcagatt ttctttgacg attttcatca tttctgcttc gattgccggc aaatcttctg
    18001 cagttacttg gttttgtcca ttatctgtat cgtagtaaaa ccctgaatcg atagctggtc
    18061 caacgccaaa gtggatattt ggatagaggc gacgcatggc ttgggccatc aaatgagctg
    18121 ctgaatgacg gactaagcca agtgcatctt catggtctgg tgtgatgatc tcaatacttg
    18181 catcttcctc gattggacga gtcaaatcaa cgagttttcc gttcacttta ccagctaaag
    18241 ctttttttgc taagcttttt gaaatgcttt cagcaattgc caatgttgtt gttccagact
    18301 caaattcttt gactgcacca tctggaaaag taatttttat cataatagtt tcctccttga
    18361 aatgttttga ttgatcagat catcaagctg aaaaacaaaa aaatcctagc atctcttgaa
    18421 aagagatact aggacgaaaa gttcgtggtt ccacctaatg ttcaaacgca aatgcgttcc
    18481 tcaagcgttg taacggtccg accgcccttg cttcaacaag ggtttcgaaa gtggtcatcc
    18541 tttggttttc tcctggaaaa gtttcagcct agcttttcct ctcttatcga agactctcaa
    18601 attcagttgg ctttctcatc aatcaattat gtgtttattt aaccactaat aaatagaaaa
    18661 atcaagtggg aaactaaact tattttttcc attccccatt tacaccgtat gttccttttt
    18721 tcatctcatt gatgatgaca tggatgtgtt cttttggtgc ctgcgtgttt ttatgaacag
    18781 cttccgtgat atctttcatc atattttcta attgttccgg actgcgacct tctactaatt
    18841 cgacatgtac gaatggcata atgctctctc ctctttctga tcgttctttt ttcaactcga
    18901 ttctatcata ctatgtttgt cttgggtact gaatcaggaa aaatggctgg atcttttttt
    18961 gctctcaagg cactcaaaat agagaccgta tccacgactt cttgcaaaac agctccgaat
    19021 aatgcgggga ttagtcccgt acttgcaatt aacatcaaaa aaatacaaat aaagatgcca
    19081 atcagtacag actgtttcgc tattctcatc gtatctcttg aaatcactac tgcttttgct
    19141 acacgcaaga gatcatcttt caagatgacc gcatctgcac tttcactagc agctgtagaa
    19201 ccatgagccc ccattgcaat accgatatct gcagcagcaa gtgctggtgc gtcattcacg
    19261 ccgtctccta ccatcgcaac aggtctttga tctttaggga tactttctaa aatggtcagt
    19321 ttatcttgag gcaagctacg ggcatgtaca gaatctaatt ggactttttc tgcaatcgtt
    19381 tgagcaatgg cagatccatc cccggtgatc atcatcaaat ttttcacatc aagttttctt
    19441 agctccttga ttgtctttgc tgcttctggg cgaatctgat ccgaaaatag gatcgttcct
    19501 gcaaaagatc gatcaattgt tacatagatt gctgtattgg acgtttctat ttcttcagct
    19561 gaaacaaaag aggcacgtcc aatctttact tcatgattat tgactactgc ttggattcct
    19621 tgaccggtca cttctttcag ttgactaaca ggatacagtt ggctgttttt cacgttgttc
    19681 actaacgaac gagctaagat atggctagat tcctgttctg cactagctgc atacagaagt
    19741 agctcttctt tttttattcc attgactggc tgaatcgcct ctacttccaa tatccctttg
    19801 gtaagcgttc ctgttttatc aaaagcaatc gtttttgtac gtgcgagctt ttcaatcgtt
    19861 gttccagttt ttacaacgat cccgttttta cttgaacggc tcattcctgc aactaaagca
    19921 atgggcgcag ctagaatcag tggacaaggt gatgccacta ccagtacctc agcaaatcgt
    19981 aaagggtctt ttgagaacca ccaagaaata ccaccgatca aataagcaat caacgtgaaa
    20041 gggactgcat aacgatcagc taaacggaca aatcgggcag gcttttcttt tgattcttcg
    20101 actagtttaa taatcatttg atactggctg tctgcggcac gtttttcagc gatcatttgg
    20161 atcgatccgt ctccatttaa agaccctgac ataatctgat ctccaggttt cttggtttcc
    20221 gggcgggact cacctgtcaa agaagattca tcgaccgaag attctccttt ggtcactcga
    20281 ccatctgcgg gtacaagttc gccaggcttg actagtaact ggtcatcaat atttatttca
    20341 tcgattacta tatcctgcaa ttttccttct ttgaatcgat gggcaattct tggggtgtta
    20401 tctaacaaag actgcagttc tttacccgct cgtttattcg cgtaatcctc tagactatct
    20461 ccgcccgtaa gcatgatcaa tatcatcaaa ctagcccagt attctttgac ggctaatgtt
    20521 gcaaggattg cggtaatcgc taatacatct acgccatatc ttcctgaacg taacgtcttg
    20581 atcatttcca aaaacatcaa gaaggcgaga atccctccag taaatgcgac aatcgcaaat
    20641 gcaagggccg gctggtgaaa aacaaattca cttaataaag ctatcccacc aataagcaaa
    20701 gtaagtaaaa attttttgaa atgtgtcatt gttttttctc ctcttttatg tgacttagag
    20761 aggttagtaa taaatttcca gctgcctctt tcatcttaag catagtcctt gactagcact
    20821 ttttaaaaca ataggcctta attgaaaaca actatcacta tcagcgtttc caaaattcat
    20881 cagttctaca aacaaaaata ttcagcaagg attacttttt cgattcatca gttaaaaagt
    20941 accttttttc gaaaaactat gctatacttt taattcgtta tttacggcgg aaggagcaaa
    21001 catgcgatga atgagaaaca actagaacaa acgcacgtgg atgaaacgat cgaactaatc
    21061 cagatcgaac aaaatatgct ggatacaaaa caacaagaat tgactcagga aatgaatcat
    21121 tcgacaaaag acacagctaa tcacaagatt cgagctggtt ccaatgaagc tttttatgaa
    21181 tctgctgttg aatatcgcca acatgagcag gaattgcttt tgaaatacca tactcttgaa
    21241 gcacagcaaa aacgcctgca aacgttgtct gtcatgaaag atagtcctta ttttgcccgt
    21301 atcgacttta aagaagaccg tgaaacagaa acattatatc taggtatcgc ttctttaaga
    21361 gatctggcag aagaaacaat cgttattgac tggcgcgcac caattgccaa tctttattat
    21421 gaaggtgaga ttggtcccgc attttatgaa actgaaacag atagaataga tgtcgatctg
    21481 cagttgaaac gacaatttaa aatccaagaa ggacgtattt tatctatggt agatacgtcg
    21541 gaagtaatca atgatgactt tttgctagag atattggatg aatcatcaag caaccatatg
    21601 aaaaacatcg tctctacgat acaaaaagca caaaatgcaa tcattcggga cacaacaagt
    21661 aaagtaatgc tgattgaagg aatcgccgga agtggaaaga catccgcttt gcttcaacgt
    21721 attgcttttc tgttgtatca taatcgcaag tggctcgagg ctgaaaacgt tcttctattt
    21781 tcacccaacc atttgttttc tgattacatc tcgactgtat tgccttcatt aggtgaaagc
    21841 ggcgtgccta cacagacatt caaaaattat ttggtgcagc tgctgccaga gttttcttta
    21901 ctagatgaac agcaacaaga aagtggtttt ttgctagggg aaaaagatcc gatacaagtg
    21961 atgaaaagcg gattgacctt agtcgatcag attgatcgct atatccaagg catcacaaac
    22021 tacggtccct tgttccgtga tatgaaaata aatggccgta cgatcctatc aaaggaatcg
    22081 attcgccaat ggtataaaga aacaaatgaa caattacctt tacatcatag actttctcta
    22141 ttacaaacga agctcctgaa aaaattaggc ggtctgcaaa aagacgaaac acgtcagcaa
    22201 tgggtcaaag atctggcgga agaacagctc caagaactat acgcaacaga tccaaatctt
    22261 gaatatacag aacaaaagga gcgttcatta cgtaaaaaac tggccaacca aatcgtacgt
    22321 aaaagattcc gtcgaatcca tcgagggatc atgaattatc aatttgtcaa tataacgaaa
    22381 caatatctcc actttctaca aactgtgcca ctttctcttc taacacgaca taaaatcagc
    22441 gagaccaggt ggcaggaaca tttgacaact gttcgtacac acttgcgtca aagagaattg
    22501 cgtcaagagg atgctgtact attcttttta ctgatgcgcc gaacatatcc agtgacggtt
    22561 acacacaaag ctcgctatat ctttattgat gaaatgcagg atttcgcacc atcacaagtg
    22621 gcattgctgc aagccttgtt cccaaaagca aatttgactt tctgtggaga tctaaatcaa
    22681 aatgtctttg gaaatgaaac gatcgttgga tcattggaag accttttcgt ggaccgtgaa
    22741 gtcactcgtt tccaattaac gacaagctat cgctccacaa aagaaatcac tgattttgct
    22801 aatcatttta ttgtgaaaga acatcaagta gaaacaacag caagaaaagg agaccttcct
    22861 ttgctcatcc aaggaacaac agatgatatc aaattgaatt ggttagcaga taccattcga
    22921 acgactcagg gaaaagcccg gtattggcgt acagcaatca tagctaaaac acaagaagaa
    22981 tgcgaagctc tttatgctca actgccagac gagttgaaac aacaagtcca gctgatttta
    23041 aatgagagtg actttatgaa gcgttccatt attttacttc cagccttttt agcaaaaggt
    23101 ctggagtttg accgagtttt cttgtgggat gtagatgcag aacatttttc ttctcagcaa
    23161 gacaaattga tcctttacac catgtgtaca cgtgcgatgc atgaattgac cctttttacc
    23221 tccaataatg aaagtccttt gttaaacaat atggatattt catcttatga gaaaaaagta
    23281 ctgtaagtaa aaagacaact gctatcatta atgttagcag ttgtcttttt atttacaata
    23341 ataattgcag aaaaagtaat tcaatcattt aaggtcattt attctatata taaatccaaa
    23401 tctgtttcag gatattttaa tttgacaagc gaaataatca ttttcagttc ttccatgttt
    23461 tctggatatt ctgggtcaaa taccttgcta tgacggccaa tcccgccatt gtcgatcaat
    23521 cggtaaattc gattccattc aatgaattgg tcaattgatt ggaaattgct gattggatag
    23581 ccgccacaat ccaaaatctc tgatacacga aatgctaggt atgcactatc gatcacttta
    23641 ttttttttcg tgtctatctt ctcatttctt tcgataagaa ttttttcagg acgatgtttc
    23701 aagctccact ctaatagtag aaagaaagcc tccatccgat actcttcttc aaataaccga
    23761 aaggctgaat cagaacgacg aaaaagcagc ggaatcagat aattttgtct ataaatcagc
    23821 attggtgcgc ggtccaccat acactctaaa taacggttca gataccctgt accgacatag
    23881 tttcctaatc gatagccttc aacgccgaat cttgcgtatt tttgcgctaa ttttgaacaa
    23941 tcagttacgt tcacagctaa ctcttctact ttgcggtaac cgcttaagat acgttcaaaa
    24001 ggttctaaag tgatttttgc ttccatattc ttccccgtcc atcttccaca tacttcatat
    24061 catttcacat gatacttatg ctctcatcat accacaggtt gaattagaaa agaaaataaa
    24121 tcacacaaaa aagcaagaca atctgttagt gagattgtct tgcttttcga aaaagactga
    24181 atacttttcg ttaaaactgg ttgcttagtc ttctaatgcc acattatgga acacatgctg
    24241 tacatcttct agatcttcca gtgcatcaat cattttttca aatgtttctt gatcttctgg
    24301 agatagtgtt acctcgtttt gagggatcat ttggatctca gcgattgaaa aatcagtgat
    24361 tcctttttct tttaatgctt cttgtatact atggaaatcc gctacttcac cgtaaacgat
    24421 gatttgtcct tcttcttcca ttacatcgcg aacatcgatg tctttttcca ttaggtattc
    24481 aaggatctcg tcagcgtctt ctcctgcaaa agcaaagatt gctgtattgt caaacatgta
    24541 agaaaccgct ccggaaacac ccatgttccc gccatttttc ccaaatgctg cccgtacgtc
    24601 tgctgctgtt cgattcacat tgttcgtcaa tgtatcaaca atcaccattg agccgcttgg
    24661 tccaaatcct tcatagcgca attcagagta tgtttcatct cctgcacctt ttgctttttc
    24721 gattgctcgg tcaataatat gttttggtac attataagtt ttagcgcgat caatgacgaa
    24781 acgtagtttt tggtttgcat gcggatcagg atcacctgat tttgctgccg cgtagatttc
    24841 aatcccaaat tttgcataaa tccggctatt attagcatct ttagcagttt tctttgctac
    24901 gatatttgcc catttacgtc ccatattttc acttcgcttt ctgatttgat agtttcataa
    24961 cgcaaaaacg tcctgttcca ttatacttgt gaatcacgat tctgtgaagc tttttttctt
    25021 tcttccttca ttcttctcat ttttagtcaa ttatctatac tcggattggg atttgcccga
    25081 aattctgttc cggaatattg cctcccaatt gatagatttg atcacttcta actaacagtt
    25141 ccgcttcttg tgcctctttt tttccgattc gggaataaat agaaatctct tttttttctt
    25201 ttaagaatct tcttccagca tcggtaaatc caagtagtcg aagataattt ttattttgtt
    25261 ggacaggaat ctcttgcttt ttgatattca gcaagacata acaagataat cgttggatcc
    25321 gtgtccatgt atatcttttt gtttttacct ttgacacaaa atcctcaaaa ctatcagcag
    25381 ttgcagctgc tgttttcatg cgcgattcta ttccttcggt catctgatag atatctgtca
    25441 gttccagcaa agtagaagat aacaaccgat aacgcaaata cggccaataa gattcccaag
    25501 tctgcagcga acaagttgaa agatcttctt ctgtctgagg cggcacaagt ccagctattc
    25561 tttttccttc ttctaacgca tggcgaatgg ctgttgcact ggctatcctc tgatctgtga
    25621 tgattttttc atgatagcct gcacttttac gtcggatcgg atgtaattcc atagggtgga
    25681 catacttggc attttccttt gcataactta atcccaagat atgatttggc gaagaaaaat
    25741 caagcgtaat atcgggatac agactgcgaa aaacctcagt cattttttgc ggataactat
    25801 acgtttgatc agacagttct tgaaatgcct cttctatcct atcttgattc tcttggacaa
    25861 agcgtccgaa actgctgtaa tcaaacgaag tctcttcttc tgttccaaaa cacaaactgt
    25921 cacagtccag tgtttgcaag atttttactg cacctttagc aaaatagtca gctgactgaa
    25981 ccgcccattc gacgggaagc tccactacta aatccacacc attttgtaaa gcttcattcg
    26041 cgcgatgcca tttatcgatg atagccggtt cgcctctttg caaaaaattc ccgctcatta
    26101 cagcaatgac gacctctgct cctgttattt ttcgtgccat ttccacatga tagcgatgtc
    26161 cattgtgaaa aggattgtac tctacgataa tcccgcagct tttcatgcgc atcctccttc
    26221 atagttatcg ataaaaaaga aatagagaca agtaaaaaaa ggcaaaaatc ggaaaaaata
    26281 cccgaactat ataaactatt ctcgcaaaca caatacttct gcgacgaaaa gctgcactgc
    26341 aagagcaatg cctatatcgt tcggatatat aatttatgtt gaagatttat tcttttcttg
    26401 ttaactcgag caggcaaaac cgctagtctt tgtatgctac aaagaaccag cgctttgatt
    26461 tctctgatgc tgcttcgtct gtaaaatcag aaaatgcttc gactttcgaa aagccgctgt
    26521 tttccagcat tcttaaataa ctttccaatg gataggtacg ctcttgatgt agttcatctt
    26581 cacgaataaa tcgaccagac ccttcttgtt cttcaacgaa aaaggtaaga aaatgttcga
    26641 tgctgtgttt ctcgcttcct ggataactat cccacaaaaa agcaaaatta tccccttgat
    26701 aatgatagct atattctgga aaaacctcat ctacttgata ggtggagtgt acgtcaaata
    26761 aaaatcggcc attttcttct aacgcttgat agacttcatc aaacacttgc tgtacctctt
    26821 gtctattttt catatagcac agcgaatcag aaaaacaggt aattgcctga tattgtccga
    26881 tttctgataa atcgagcata ttgccttcca caaattggat cgatgcgtct tcttcatatg
    26941 ctcgctggct ggcaatcatc aacatctctt ctgaaaggtc taaccctgtc actttatatc
    27001 catcttttgc aaaggcaacc gctaatgctc cagtaccgca tgccagttcc aaaatatttt
    27061 ttgtgtcttc ttgtaaatga cgtttcgaaa aagctaacca ttgctgataa agtgtttcgt
    27121 ccatcacttc atcgtaaatg aaagcaaatg tttcataagc catatgttaa tccaaccatt
    27181 cactaagatc gactagcgga gcatctgacc atagtttttc cagattatag aaatttcgtt
    27241 ctggcgcatg gaaaacatgt acgatcacat ctccaagatc gatcaatacc cattttccgc
    27301 cttcttttcc ttcaatacgt tttacttcat atttgttttc ttcttcttta tcaatgattt
    27361 cttctgtaat tgcgttgatt tgacgttcgc tatttgctga acagatcatg aaataatctg
    27421 ccaacaagga aacatttcga acgtctaaag cgataatgtc ttctgcccgt tttgaatctg
    27481 ctgcttcaat cgcaattttt aattgttctt tactatctat gatgatttcc tcctaatatg
    27541 agcagcttat ttttttgcta cccagtaatt atatgtttcg attgtttttg gataaatctg
    27601 ttgttcttgc gcaagcaaat gggccaatgt atgttttgtt tcaaatgcta cagcttcgtc
    27661 tagatcagcc catgcaatgg ctcgtgcatc ttgtacccca ggaaaatctc gacctggttc
    27721 aatatagtct gctacataaa tgattttgtc aaggagactc atcttagcag ccccagtcgt
    27781 atgcacccgg attgcatgaa gaatctctgc atcggtgatt cctaattccc gctctacaaa
    27841 cgaagcgcca acaagaccat gccagattgc attattataa ttcaataact ccggatcaaa
    27901 accgtctctt acgattacca ttttgaactc ctcatctggt cgttcttttg cataatcatg
    27961 tgtcaatgca gcaatactag ctttttcagg agaagcaccg tattttttgg caagagccac
    28021 ggctgtttct tctactccaa gcacatgctt gaagcgttgt tcgctcattc tcatctgtat
    28081 tttttgcatc aggttttctc tagaataagc agtatattca ttactatagt ccatattcat
    28141 acaacccttc attttgaata taatcaatta ctttatctgg aacaaggtag cggatagaac
    28201 agccttcttt gatcttttga cgtattttcg tggaactgat gtcgatctca ggtacatcta
    28261 cccaaattac aggatatgga gtatccgttg tgtatcctgg tcgacgtata cccacaaagt
    28321 tgaccataga agtcagctca tcgattttat accattttgg caaatattct accatatccc
    28381 caccgataat aaagtaataa tctgtatccg gattgttttg cgtcaattcc ttcatagtat
    28441 cataggtgta acttttccca ccacgagcta attcaatcgt ttcaatctgc aaaaaaggat
    28501 tgtcttccac cgctaattcc aacatgttga gtcgatgttt tgcatcaatc gtctgtttct
    28561 catcaacatg aggtggctga taagaaggca tcaaaaatac gcggtcaaga cctaaattcc
    28621 ggccagcttg ttcagccatc actaaatgtg caaggtgaac gggattgaat gttccgccca
    28681 agatccctac ttgctttttc ttctctaaga attgaggcat ttcttcagga aataattgtg
    28741 aacgaacaaa tgtcttagct tgcgtattca tcatggtcac tccttatatt gcttttactt
    28801 cttttgatag cctttgatac ttttcttttg tggaaggttt gtataataca ataacacgtc
    28861 caatgatttg gacgatttcg caatctattt ctttttctaa tacttgagct gcttcttctg
    28921 caatctcatc tgtattttga agtaacgata ctttgatcag ttctcttttt tctaaagctt
    28981 cttcaatttg aacgatcatt gctgaattga tcccaccttt tccgatttga aaaatcggct
    29041 gaagatgatg cgctttgcta cgtaaaaaac gcttttgttt tcctcttaaa ctcataattt
    29101 tccttcttcc ttaaatcaat gctttgcgtt tcaatacatc tacacccttt ggcgcccagc
    29161 ccgcaattac tactggcgct gttacagtga tccagcctag accggcaaaa acgatgtctg
    29221 ttttttcttt cacagaaaac tcaaaacgaa caagctccgg aaatgcctcg acttcatcag
    29281 gacgaggagg ctgtaataat ccacctacat gtttttgata aaattcgtct gctttttcca
    29341 gtttcgttcg atgaatattt acatcatttg acacataagc aataaatgaa gcacgattgc
    29401 cgctgataaa atcaaatctt gcaagtccgc ccaagaaaag tgtttgccct tcattcagct
    29461 gataaacttt aggtttgatt tcttttaacg gtgcaatgat ccgcaaatct tttttaccta
    29521 agtagtgcgc catttgatgg cgatggataa ttcccggtgt atcaatcaaa aaatgcccgt
    29581 catctaaagg aatctcgatt ttatccaagg tcgttcctgg aaactgcgaa gttgtgatta
    29641 catcttgtac tcccgctgta tttttgatga tttgattgat aagcgtcgat tttccgacat
    29701 ttgttactcc gacgatatag acatcccgtc catcgcggta ttcttcgatt ttttctaata
    29761 gttccgtcat ctcatgagat ttcttagcac tagttaataa tacatctact ggacgaattc
    29821 ctgcttcgta agcacgttcc ttcatccatt gtgttaattt agtttttttc aatgatttag
    29881 gtaatatatc cactttgttc ccaactaaaa gtactggatt gtcaccgaca aaacggtgta
    29941 gtcctgggat tagtgaacca ttgaagtcaa aaatatccac cacattgacg atcaatgcat
    30001 ctttttgccc taattcattc agtaagcgca aaaagtcgtc atccgtcaat gagacatctt
    30061 gggtctcatt ataatggcgt aatctgaaac aacgctgaca atagacttcc ccggaagcta
    30121 aacctttttc caaagccgtt tgcggcgtgt atcctaattc accgggtttg tttgtttgta
    30181 taacggcacc acagccgata cattgtaatt cttctgtcat tcgattccac ctcgccagcc
    30241 catatcaggg tgttttgcta atagataggc cataattctt ttttcccaaa aacgattaaa
    30301 ttgtgtcttc caactatctg ttgcgataat gggttttaca aggatgctac gtatccctgc
    30361 acgattggct ccacgtatat cagtcataat ctgatcgcct accattacta tctcatcatc
    30421 tgctaaatcc aattgtttct ttgctatgtt gatccctctt gctaaaggtt tcattgctcg
    30481 tgcaacatac aaaagatcaa atttttcaat cgctcgcgcg acacgttttg aattattgtt
    30541 tgaaacaaca acaacaggga ttcctgcatt tttcatttct aaaatccaag taagcaactc
    30601 ttctgttcca tctggattat tccatgcaat caacgtattg tctaaatcgg tcaatactgc
    30661 tttgatgcct agattcttta attgggcagg tgtaatctga tagatggctt ctaccatcca
    30721 tgttggtttg taatttgaaa acatgctgtc tcctcatctg caaaaaatga aggagcacat
    30781 aaagtactcc tcaaccccgt attatactgc atatcagcaa aaaaaactac ctctatactc
    30841 gttcgtcttt cagaaaaagc atttctttac aaaatgacca aaaataaaga ttgatcacag
    30901 aaaaatttag aaacaaaaaa caactaatat tggcggaacc tcttgtatcg tttttccagt
    30961 aatttatgct gatctaatcg aacaagttcc gtcaatcttt caatcaacgc ttttttcata
    31021 atgtgtgcga ctttatatcg tggcaactgc tcgccgttga acgtttcagg aataactcga
    31081 tcaacgattc ctaactcgcg tagttctggc gcagtaattt tcatcaactc agctgcttct
    31141 gcggcgcgac taccgtcttt ccacaaaatc gacgcaaatc cttctggaga aagcacagca
    31201 taaatcgttt cttccagcat ccagacttca tccgctaaac ctagagcaag ggcaccgcca
    31261 cttccacctt caccaatgat gatggcgatg atcggtacct ttaaatcaga catttcgagc
    31321 agatttctgg caatagcctc tccttcgccg cgctcttctg ctcctacacc gcaatatgca
    31381 cctgctgtat tgatcaaggt aatcaccggt ctattgaact tttccgcttg tttcatcagt
    31441 cgtaaagctt ttcgatagcc ttcaggatgg ggagaaccaa aatttctgcg gatattttcc
    31501 tgcagatccc gtcctttttg aataccgatc actgttaccg gtttgccatc taatcgagcg
    31561 attccaccaa caatcgcttc gtcatctgca aataaacgat caccatggca ttcctcgaaa
    31621 gaatcgaaga tctcttcaaa aaaatccaag cttgtccatc gttctttagc tcttgctaac
    31681 tgaacgacat catgtgctgt cttatccacg atctatccat cctttctgtg tatgcaattc
    31741 aagaagcgta tggattttct gtttcaattc catgcgggga acaatctgat cgacaaaacc
    31801 atgagataat aagaattcag ccttttggaa atcttctgga agttcctgct tgatcgtctg
    31861 ttcgatgacg cgtcttccag caaaaccgat caagctttgg ggttctgcta gaataatatc
    31921 gccttccatt gcaaaactgg cagttactcc tccggtcgtc gggtccgtta aaacagttag
    31981 ataaaaaaga ccttcattgc tatgccgttt gacagcagca gagatttttg ccatctgcat
    32041 tagagaaaag attccttctt gcatccgtgc gccacctgaa gctgtgaaca agacgactgg
    32101 aagactttcc tttgttgctc gttcgaataa tctggtgatc ttttctccga caatcgttcc
    32161 catacttccc atgataaaat taggatccat cactccaata atgaagggaa tcccctgaat
    32221 ggttgcttta ccagtgagga ctgcttcatg aagtcctgtt ttatcttgca tttttttaat
    32281 tttttccgta tattcgggaa atgctagcgg atctttggtt acaagatccg tatcccattc
    32341 ttcaaaactt ttttcatcaa taatcaaagc tagacgctgc catgcaccga ttcgaaaagc
    32401 gtatccgcaa tgagggcata ctttttcttc accgatgtct ttcgtataaa ggatatgttt
    32461 gcaatttgga cactttgccc acatgttatc aggaacagat ggcgagttag caggtttgct
    32521 ctctctatta ggattgatcc gaatatattt ctttttctta aacagagcca tctatgcgcc
    32581 tcctatctcc gtttctggtg tccaattcgg taaaaattct tcttgtaaaa atgctgtgct
    32641 gtaatcacct gctatgactg caggatgact aatcaaatcc atctgaaatt ctgcgttcgt
    32701 gatgattcct tctgtgataa attcacttaa ggctcgctgc atcttcatta atgcttcaaa
    32761 acgcgtattt ccatggacga ttatttttgc aatcattgaa tcataatatg gcgggatcgt
    32821 atatcccggg taaactgcac tgtctactcg cagtcccatc ccacctgctg gaagcagtaa
    32881 gttttggatc ttccctggag aaggagcaaa atggaaagca ggattttctg cattaatccg
    32941 gcattcgatt gcgtgtcctt ggaatactac ttcttcttga cacacattca aaggctcacc
    33001 ggctgctatc tccacctgtt tttttactaa atcgattcct gtgaccattt ctgttactgg
    33061 atgttccact tggattcgag tattcatttc catgaaataa aaatcgcctt cttcatccat
    33121 cagaaactcg atcgttccgg cattttcata attgactgct ttagctgctc gaacagcagc
    33181 gtctcctagt gcttctcttt ttgtttgtga gatcactaca gaaggagatt cttccagtac
    33241 tttttgattg ttgcgttgta aagaacaatc tcgctcgcct aaatgaatga catgaccaaa
    33301 tcgatcacct aagatctgaa cttctatatg gcgagcagga taaatgattt tttccaaata
    33361 catatcatca tttccaaatg ccgctgctgc ttcttgctga gctgaagaaa aatgctgtgg
    33421 gagctcttct tttgattgaa ctttccgaat gcctttcccg ccaccacctg cagctgcttt
    33481 cagcatcact ggataaccaa ttctgtctgc tatttccaat gcttcttcta ctgtagataa
    33541 tacaccagta cttcctggaa tgactgggac atttgcttct tgcatcaatt gacgcgcatt
    33601 gatcttattt cccattgcat cgatcgttgc acttttagga ccaataaatg tgatattgca
    33661 ctcttcacac atggaggcaa atcggctatt ttcagataaa aaaccaaatc ctggatgaat
    33721 tgcttcggct tttgtcacga ttgcagcgct cagtacttcc tgaacattca gatatgaatc
    33781 aacggctttt gccgggccaa tgcagattgc ttcatctgct aactgtgtat gaagtgcttc
    33841 ggcatctgct tctgaatata tcgctacggt ttggatccct agttcgcggc atgcacgaat
    33901 gatacgtaca gcgatttcac cgcggttagc tatcaatact ttagaaaaca tatttcacct
    33961 atccaatcat aaaggttaat tccgcttcgg ctactttttt accgtctact ttagcgattc
    34021 ctttgcctat tccggctgaa gctttcactt tcaatatctc aacttcaagg tataaggtat
    34081 ctcccggtgt aactttctta cggaattttg ctttgtccaa tccgccgaaa taagccgtct
    34141 ttcccttgaa ttgttcaaga gaaagtaatg caactgctcc tgcttgagcc attgcttcaa
    34201 caatcaatac ccctggcata acaggttctt ctggaaaatg tccttgaaaa aagggctcat
    34261 tgatcgttac atttttctta gcaacgatcc gttctccttc aaccatttct tccactcgat
    34321 caatcaaaag cattggataa cgatgtggaa tgatttcttt tatttcttgt atgttcattg
    34381 ttttccctct ttcctgcttt gcttattttt gctgaatcct aaagagtggc tgtccatatt
    34441 cgacaactga ctcgttttct acaaggattt ctgtgatcac tccgtcggcc tcactagtta
    34501 tctcattcat cagtttcatc gcttcaataa tgcagataac ttctcctttg gcaacttgat
    34561 cacctacttt tttgaaagct ggtttgtcgg gcgctggttg cagataaacg attcccacga
    34621 ttggcgaaac aacctcgatt ccatcaaata ctgccgattc tttttctttc attatttctg
    34681 gagcagttgt tgcttgttca ggtatgacat tgactggctc cgaaactggc aacgtttcgt
    34741 ttgtttgaac catattcatg cctcgacttg ttttattttt attcatatac aattcaaatt
    34801 gaccttcacg caaatcgaat tccgttagcg aggattgatc aaattgactg actaattctc
    34861 tgatttcatt gatatccaaa attattcctc ccagcgtttc atgcacagaa ccgcattatg
    34921 tccgccaaac cctaatgaat tactcattgc ataagtaaaa cttgcaggtt tgctttcgtt
    34981 tttgacaact gtcaagtcaa tctcaggatc ttgtttttct acattgatcg taggtgggat
    35041 aaattgatgt tctaatgcca gcaaagttgc caaggcttcg atcccgccgg ctgctcccaa
    35101 taaatgtcca gtcatcgatt tcgtgctgct gacgtaagta tctctgaaat tttcacctaa
    35161 tgcgatttgg attgcttttg cttctgcctg gtcattggct ggtgtacttg ttccgtgagc
    35221 attgatatat ccgacttgtt ccggctgtac accagcttct tccatcgcaa gcttcattgc
    35281 tttagatgca ccagaaccat ctggagtagg agctgtcata tggtaagcgt cacagtttgc
    35341 accgtatcca acgatttctc catagatttt ggcaccgcga gcttgtgcat gttctaaaga
    35401 ttcaagaact agaacgcctg ctccttcacc catcacaaaa ccattccgat tttcgtcaaa
    35461 cggaatcgaa ccacgattag gatcagttga ttctgagagt gctgttaaag ctgcaaatcc
    35521 agcaatcccc atctcacaaa tcgttgcttc tgttcctcca gcaagaatga catcagaata
    35581 gccatgtttg atgttacgga atgcttcccc aattgaattc gttccgctag cacaagctgt
    35641 tacgatagct gtacaaatac cttttgcacc tacacgcaaa gcaatatttc cagctgccat
    35701 gttggcaatc gccattggta cgaacattgg agaaactcgt tttgcacctt tttcatgtag
    35761 acgaataact tggttttcga ttgttggaag accaccgatc ccagatccga tgatgacgcc
    35821 aaaccggtct tgattcattt tttctttatc taaaccgctc atctccattg cttctaaagc
    35881 agcgtagatt ccatagatcg aaaacatatc cattcgtttg ctatcttttt taataaaata
    35941 tttatcgtaa gggaaatctt tgacttcacc cgctaaggta atccctgttt ctgaagcatc
    36001 aaatttagtg atcggtgcga tgccattcgt cccctctttt aagctattca aaaagctgtc
    36061 tgcgtcattt ccaataggtg aagtgactcc atagccagta attaccactc gattcatttg
    36121 tttgctctcc ttttcttatc cgtgcatgac caatccgcca tcgacattga tgacttgtcc
    36181 ggtgatatat ggattttgtg ctaagaaaac tgctgtttgt gcaatatctt ctacttgtcc
    36241 gaaacgctgc atcggaattt gcttttccgc ttgttccttg actttgtctg ctaaaacttc
    36301 cgtcatatca gttgtgatga atcctggcgc aatggcatta catgtaatgc ctctagttgc
    36361 agcttctcgt gccacagatt ttgttaatcc tactacgcca gctttactag ctgcataatt
    36421 tgcttgacca atatttccaa tcaacccaga tacactagag agattaatga tggccccttc
    36481 tcgctgcttc atcatttttt tcaatacgtg ctgcgtcata ttgaaggttc caatcaaatt
    36541 gatatccaga cattttttga aatcttctgc atccattcgc atcacaagtt tgtcatttgt
    36601 gattccggca ttgtttacta acacatggat cgatccaagc ttttcttctg cttctttgat
    36661 catttgtccc gctttctcat aatcagagat gtcaccggaa actcctacgc attttacacc
    36721 gaatgcttcg atttcttcta tcttttcttt tggaatctct ccgcgaccat tcaaaatgat
    36781 atttgctcca gcttttgcaa aagctaaggc catcgctttg ccgattcctc ttgtactacc
    36841 tgtgataaat acattttttc cttttacatc cataacgttc ctccttatag tattgctatt
    36901 gtttcatcaa atgtctgctt attctcaaca cgcagaactg gtattgtttt atcgattttt
    36961 ttcataaatc cgcttaatac ttttccgggg ccgacttcga tgacttgttc gatgccgatt
    37021 gtttttaact tatggatact ctcatagaaa cgaacaggtt gcatgacttg ctgttcaagt
    37081 agtcccgcga ttgtttcttt cttcattatt tccgttgttg tgttgcttat gataggaaaa
    37141 gaaggttcag agaattgaat ctgacttagg tccttcgcca gtttttttgc tgccggttct
    37201 aaaatagctg tatgaaaagg cccgctgaca tttagaggga tcattctttt gaaccctttt
    37261 tcttttaata gtaaaacagc ttcgtctact gctttttctt cgccaccaat cacgatctgc
    37321 tgaggtgtat tataatttgc aggagatacg atgccgtact tgctagcttc atgacagctt
    37381 tcctcgatcg tttcaatcgg cgcattcatc actgcaacca ttttcccgct tccagctggg
    37441 gcagcttctg tcatataagc gcctcttttt gctactaacg caactgcttc agtgaaggat
    37501 agcgcattac tagctactaa tgccgaatat tctcctaaac tcagtcctaa agctgcatct
    37561 gggatgattc catgctcctt caataagcga taatacgcga tgctgactgt tagaatagct
    37621 ggctgtgtat attgcgtctg atccaaacgt tcattttctg taaagcaaag ttcagccata
    37681 tcgtagccga gaatctcact tgcttcatca aatgtctgct ttacaacggc ttcttcatat
    37741 aaatcttttc ccataccttg atattgtgca ccttggccac taaataagaa tgctgttttc
    37801 atgtttttct ccaatatatc cagaatatat gtttgattct tcctattcaa aaaggccgag
    37861 acattcctat ttcaggaaat gtcccagccc ccaacctaat tttaatgatt atgcagtttg
    37921 tttttctacg taagtcacta aatcttgaac cgttttgatt ccgtcttctg tttcgatttt
    37981 cacgtcaaat tcatcttcaa tttcattgat gatttggaat aaatccaagc tgtcagcttc
    38041 taagtcttct tggatgtcag tagtcaattt gatttcttca gtttcttttc ctaattcttc
    38101 tgcgatgatt tcttgaattt tttcgaatac cataataata tattcctccg atattgtttg
    38161 ctttttattt ttataattta acagtctaca actccatgag aatacttccc catgttaaac
    38221 ctccgccata gcctgtaaat attactttct gctgttttcc taaggtgatc tttccactag
    38281 aaactgcttc atccaataag atagggatgc ttgcagcaga tgtattccca tatttatcca
    38341 tattggtcag gaacttttct tgcggcatct ttacttttcg ggcaattttc tcaataattc
    38401 tgaggtttgc ctgatgcaaa agcagataat caactggttt gtctttcatg acttcttgga
    38461 tattgtttgc aacgtctcgt acggcaaaat caaaaatatc tctgccagtc atttgcaaat
    38521 aatacgaacc ttctgaatct tctgaataga acggattttt attttgctgg tagccagcgg
    38581 tcaatgagct acttctggta ccgtcagctg ctaatttttc tttcagcaat tttttctgtg
    38641 gattcgcttc taagagaatc ccacctgctc catcgccaaa caacacagca gtcgtacgat
    38701 cagaccaatc gattagctta gatagtgttt caccaccaat caccagtccc ttagttgagc
    38761 cgcagcggat cattttttca gccatgctta acgcataaac aaatcctgag cacgcagcac
    38821 tgatatcaaa agcgatagca tgagttgctc ccaatttccc ttgtacttgt gctgctacag
    38881 atggtgtcgt gtaatcgggc gacattgttg ctactaaaat aaaatccaaa tctttagggt
    38941 ttagattttt ctgttttatc aatttttccg ccactaaaat gcataaatca gaagtctcct
    39001 gatttgttac acatcttcgt tcacgaatac ccgtccttga cgaaatccat tcatcagagg
    39061 tatccatcat cttggaaaga tcattattag taataatctt ttcaggaagg acgctggctg
    39121 ttgcagtaat cattccaaat tgttccatta aatatcctca cttatagtct tgcaagaagt
    39181 cgtgcaagtt tttcagtgca tccagcaagg catgctgttc ctccgtagcc attccgtcta
    39241 agatgttctt gaccatttca cgatgaaaat gctgatggac ccgaaatagt aactttcctt
    39301 ttttcgttag tcccaacttt acaacgcggc ggtcatcttc actgcggata cgttcgacat
    39361 agcctttttt tactaacttg ttaatagcag tggtcaaggt cccaacagta attgatagtt
    39421 ctttggctac ttctgaagtt gtttttttct tgtacatccc gattgcctcg atcgtgtgca
    39481 tctctgtgat tgacagatct ttgaattgag attttttcag ctcagactct tcgattgtca
    39541 aaatatcatt gaagacacta acaagataat catttactgt ttttaaatct ggttccatgt
    39601 tccaccgtcc actaattagt ttgattatca aatcttttga tgttcaaact atatcttaac
    39661 ttttttcaaa atgcaacctt aaaagatcat ttttttacta tatgagcttt ttttaagatt
    39721 ttactcattt aaaacaactg ataaatcagc atttataatt tcttttatta gaaaaatcat
    39781 ctatctcaca tattttgatt atcaaagtat tttctgtgaa cagaactata gaattcccta
    39841 aaacttgtcc attatttcaa aatttgttat agtcttttta ttcaaagcgt aaaaggagat
    39901 cctatgttaa aagaaaaaat gaaaactctc cccatgctgc cgggtgtcta tcttatgaaa
    39961 gaccgagatg gtaaggtcat ctatgttggt aaagcaaaac ggctcaaaaa tagagtaagc
    40021 agctattttc atcagaataa acagcattca aaaaaagtat tacgtatgat tcatcatata
    40081 acagattttg atttcgtcgt agtagacacg gaattagatg ctttattgct cgagtgtcaa
    40141 ttgatccaac attaccgtcc tttttataac cgacaaatga attatttcag taattataat
    40201 tatgtacata ttaccaataa gggatttgta ttaacagata ctcctacggc tcgaacatac
    40261 gggccttttc gtctctataa aaaaatgccg tctattttgc gtataatgga agaaacttat
    40321 caaatgcctt ggttgtctga aattagtttg ttagctttgc gagtccagct tcctgaccta
    40381 caggaaatga cttttgaaca gaaaaaaaag gaactccaag ggctgtttca aggccgaaat
    40441 aaaaagctac tgacttactt gaaaaaaaga caacaacatt tcatctatca gttgaatttc
    40501 gaaaaagccg gtatgcttca aaaagatatc gaattagtta cttattttat tagacggatc
    40561 caggaacaaa agcagttttt aagaacacca tcgctcacct tcagcatgcc attagcagca
    40621 gatgagagtc agaaaaagca ctacctcgtt tgttatggtc aacttgcaga aacaatgatt
    40681 acctctggtg acgtttctcc tgacttctat tatgaaaaga aagaagctca tttatctttg
    40741 aaacgtcaat tgtcaaaaga agaaatcgat cctgtacaaa tcttgatcag ttaccgcaaa
    40801 aaattagaaa aagaacagtt tgaaattgaa ctgttaaaca aaaaagaagc tgaaaagcaa
    40861 ctaaattagt tgcttctcgg cttcttttag gcatcttcaa cttcaaatgt caattatcct
    40921 aaaatttatt ggattaagtc atagatttcc atcgctacga tatcgatatt atcaaattcg
    40981 tattgctgat gagtatctgt ttcagttaat tcaaatgtat cggtcgcttt atcataagtt
    41041 actgtacact tctctacgcc ttctttttca aaacggcgaa cttgcagttc attatctgct
    41101 gactcgacca tagcctccag tcgttttatg attgcgacta attgtgaatg tgtcatcgga
    41161 ggtcctcctt ttcttatttc tgttatattg tatcaaaagc atagcctact tgcattgatt
    41221 ttctctaaaa aaactcgttt tttatgattt tttttctggt atttttcctt caggccacca
    41281 aagtttgatc aaagcttggg ttccgtcatt ctcaaaacct ttatctgcca actcttcata
    41341 tagtttcaat gcttgggtag ttgctggcaa tacaagatcc attttttctg cttcttctaa
    41401 tgcaatcttt aaatctttga taaaatgttt gacaaaaaaa ccaggtgtgt aatcttcttt
    41461 taaaatacga ggagaataat tgcttaaaga ccagttagct gcacttccac ctgaaagcgt
    41521 ctcaatcact ttctttaaat caagtccgtt tttttgtgca tagacaagca tttccgtcat
    41581 tcctgtcatc gtacctgcaa tcatgatctg attagccatt tttgtatgct gacctttccc
    41641 agcagaacca tgcaatgtaa atgtcgtacc aaattctttg aataaaggca gtacttgatc
    41701 ataaacggct tcttctccac cgaccatgat cgtcagtgta ccattctttg ctcctaagtc
    41761 accaccggat accggtgcat ctaacgcatc tgcgcctttt tcttttgctg tttttgcaat
    41821 tttttctgcc aggcttggtg tatttgtagt caaatcaact aaaatatgtc ctgagatatc
    41881 tgcagaaaaa atccccgact gtccatagta gatttcttct acatctttgg ggtaacctac
    41941 cattgtaaag agaatattac tttgttctgc taccgctttt ggtgtatctg cccagatagc
    42001 tccttctgtt actaaaggat cagcttttgc ttttgtccga ttatagacag ctacttcgtg
    42061 tcctgcttct agtagatggc gtgcgacagc acttcccatg acgcctgttc cgataaaacc
    42121 aagtttcatt ttttcgacct cctaaaaatc aaaaatcctt gcttctatgc tagtatacgc
    42181 tagaaacaag gattttttca taaaaaatgt ttgaatttta tcttttaaat gaatttaatt
    42241 ttattttgat tcgttcaatc gagtcttctt cttcttttct tcgcatctct tcttcatgat
    42301 aaacaaccat gtcttgataa aattcgatca tagcttcgcc gaaatgtgta gatgaaatat
    42361 cataaagttt agcttctaaa acttttggat ctttaggaac ttcactggaa atataatcaa
    42421 taagtgctgg tgcaaagtct tcgtcggttt caaaagtttt ccctaatgaa ggatgatcga
    42481 acaatcggtt caaataagca tttccttcca cgacacaagg tgttccggct gccatcgctt
    42541 ctgtataagt aagcccttgt gtttctgaag tagaagcact gacaaaataa tctgccgctt
    42601 tataataaag tgctacatca ttgttgtcca cttctcctgt gaactgcaca aattcttcta
    42661 actgcagttc tgagatcaat tcctctaact cttctacata aggtcctttt cctacgataa
    42721 ctaatctggc gttaggataa gcggagatga cctgaggaaa cccattaatc aaggcttgga
    42781 tatttttctc ataagaaatc ctgcttaaag aaagaatcat caaatggttt tcctgaagac
    42841 ctagtgactc ccttaatttg ctgatatcct gctgagtgat atcagaacgt ttgaattttt
    42901 caatgtcgat accagtagga atcacgcgaa gcggtgctat tacaccatag ctgcgtaatg
    42961 tatcaataac acgttcactt ggacaaacta cacctgtcgt atgatttgca aataatctag
    43021 acaagtattt tacatgggat tgcctgatca cttttccttt accgatataa tgaaggtagt
    43081 cttcatacat cgtatggtaa gtatggatca gaggtatttt caacttctta gctaccatct
    43141 tcccaagtat ccctgctccg aattctgtat gggtatgaat aagatctagt tctagttctt
    43201 tggcaaccaa ataagcatac cacatgcctc taacaacaac tcttcgatcc ttgaaagaaa
    43261 taaatggtac acttggcatt cgaatcacat ctttttccaa cttatccgca ttaggatcgg
    43321 tcgttgtaaa aatatagact tcatgcccat gtctttctaa ttcttgtttt aatgttttga
    43381 tcgatgtagc aactccgctg acttgcggaa agtaggtatc ggtaaaaaag ccgattttca
    43441 tgtttttcgc ctccaaacta gtccttgacc tgatttactt tttttaattt gctagagact
    43501 ttttcattca gtacactttc gtatacattt ttaagttctt caccgatttt attgatactt
    43561 cgctcttttg ctgtttgata accggcttgt gtcgtactcg gtagttcttg attggtaatc
    43621 tgttggatca gtcggataaa ttcttcgttt gtcttcccca tatagcagtt cttgctatct
    43681 tctaaccagc cattatacac tggaatatct cgaacaagta cgttttgttt gcttgctaaa
    43741 gcttctaaaa caacgatccc ttctgtctct tcataagaag ggaaaaagaa tagatctgca
    43801 cctgaatacg caccttctat gatctctcca cgaatgtaac caggaaacag gacattttca
    43861 ggatgatctt cttttacaat tttccggatg ttccgaggaa tcgagtacat gggaacatga
    43921 ccaaaccaaa taaatgtgta ttcgggcatc ttcttagcaa tttctacgaa atcaatgatt
    43981 cctttacgtt cgaaaaacaa accgacacaa atgattactt tttgatccgg agacagtttg
    44041 aaatattctc tgaacttttg ttctttcatt ggatcagggt gatatttctc taagtcgatc
    44101 ccattggaaa tagcttgaat cggcacacgg ataccatagc tttccaacaa ctgttttgaa
    44161 taaggtgttg gcgtgatcag atagtccgct ttttggtaca atcctaccag atattttttt
    44221 acgataggag aaagctggtt cgatccgata aaggaatttc ggaaatcttc ctctgtagaa
    44281 tgcgcatgat agacaacttt ttttccttct cttctggctt tattgaccat attatgacta
    44341 ttgattccgt atgtattgat gtgcaggatg tcataatctt ctttttcatc tagtgtataa
    44401 gagataccga cttccgtcag agcacgtttt tgatgatcaa gcgcacgccc gattccagat
    44461 tttgcaagca tcttttcact ttcaaaatat aataatacct tcaccttacg tcccccttca
    44521 cttatatagt ataacatagc ctacaaactg tcactcaaac ttaagcccta tttttgtcta
    44581 ttcttactga caaaaacgtt ttcttaaaaa agaacaaaaa aactggcgta gctcttttac
    44641 aagccacacc agcttttgat acgttgttta ttttacatat tcatgaacaa gttcaacgac
    44701 ttcttccatc atatcgcatt ctttcaatgc acgatcagca agttcagcca ttttcgctgt
    44761 gtccaagcgt ttcattaaac tacgcgtttt aagaatagat gtcgcactca ttgagaactc
    44821 atctagaccc atacctacta gcaatggaac agctgtttga tctccagcca tttctccaca
    44881 catacctgcc cattttcctt cagcgtgtgc tgcatcaatt acatttttga tcaaacgtaa
    44941 aattgatggg ttatatggtt ggtacaagta agaaacgcgt tcgttcatac ggtctgctgc
    45001 cattgtgtat tgaatcaaat cgttcgtacc tacagagaag aaatctactt cttttgcaaa
    45061 cttgtctgct aagactgctg ctgctggaat ctcgatcatg atgccgactt ggatcgtatc
    45121 agatacttcc ttgccttcac ttatcaattt ttgtttttct tcttcaaaga tcgcttttgc
    45181 tgcgcggaat tctttgagtg tagctaccat agggaacatg atacgtaaat taccatgaac
    45241 agatgcacga agcaatgcac gcatttgtgt acggaacatt tcgtcgcctt gttcagataa
    45301 actgatacgc aatgcacgat atcctaagaa tggattcatt tcatgaggta attgcagata
    45361 tggtagttct ttatctccac cgatgtccat tgtacgaaca actacaggtt ttccttccat
    45421 accttctaat acagctttgt aagcttcata ttggtcatct tctgttggga agtctggaga
    45481 atccatatac aggaattcag tacggtacag accaactgct tctccaccat tattatggac
    45541 accaactaga tcttttggtg ttccgatgtt tgcagctaat tcaaaatgct tgccgtcagc
    45601 tgtgacagtt tctgcatttt ttaatttttc ccattctgct ttttgtgctg cgtaatctgc
    45661 acctgctttt tcaaattcag ctttttgttc atctgtagga tcgatgatta catcgccttc
    45721 gatcccgttg acagccaaaa tatcgccttc tttgacttta gcagtaattt cttttgtccc
    45781 aacgattgct ggaatttcta aagaacgtgc catgatagca gaatgtgaag tacgtccacc
    45841 aatgtctgtt acaaacgctt ttacaaaatt gcgatctaat tgtgctgtat cacttggagt
    45901 caagtcatgt gcaacaacga ccacttcttc gttgatcatt gatggatttg gtaaagtaac
    45961 gcctaataag tgcgctaaaa tacgtttagc aacgtcacga atatctgcag cacgctcttg
    46021 catataagca ttatcttcca ttgcttcgaa cataccgata tacatatccg ttacttcttt
    46081 tagcgctgat tcagcattta cgctgttgtc tttaatattt tgcttgattt gacctaccat
    46141 ttctggatct gaaagaacca tcaaatgggc atcaaatact tgcgcttctg cttcacctaa
    46201 gctttgcgct gctttttcac gaatttgctg caattcttca gtagatttag ctaatgcagc
    46261 atccaaacga gcttcttcag ccgcagtatc ttctactgtt gccttactga atgttaaatc
    46321 cggttgaact agcaggtaag cttttgcaac ggctacaccg tcactagcgg caatcccttt
    46381 tagcatttca accattattc agataagcct tctttcttca ttgtttcaac gattgctgcc
    46441 attgcatcag cttcgtcagc accttctgca gtaatagtaa catcagaacc ttgacctaca
    46501 cctaaagaca taacgcccat gattgatttc aagtttactg atttaccttt atactctaag
    46561 tttacatcag agttaaattt gcttgctgtt tgtactaata gtgtagctgg acgagcgtgg
    46621 atcccagttt ctgctactac gtgaaattct ttcttttcca tattaatcgg tctcctttaa
    46681 gtgagtgttg tttttatgtg agggttttct tttttagtaa ttcagagaat cctaacaaat
    46741 gccacccttt catctgtaaa gattaacatt ttttttcgtt agatacaaca gttttcattc
    46801 aatttcagat aaaagtattc aaattattcc ggattctttt tcatcctttt cttcacattt
    46861 tataacaaac gtgtttattt cctttttctt gtcgctttct cttctcctga cgtttatcat
    46921 tctttccttt gaccctcttt gttttcatat gaaacgaatt acttgcttcc ctttttattt
    46981 ttagtataat aagagtcaaa ggtcaggaat ggtcaataga gatttcacct tttctacctc
    47041 ataaatctct taaaaaacta ggggttattc ttttacctca tgaaaaacga gatgtaaaat
    47101 atggatgtac ccatttttgt gaaaggacgt gaatgattca tgctttgtca aaactgcggc
    47161 aagaatgagg caacgattca cttgtatgcc actgtcaatg gtcagcggac acaactcgat
    47221 tactgtcaga gctgttatca aaaaatcaaa aatcaacaaa atggaggtct gactaatatg
    47281 gcccaaaatg atccatttgg atttggcagt ttggacgatc ttttccgttc catgtctcgc
    47341 caaatgcaac agcaaggaaa ttatgagcaa acaccaccaa cacaatttgg cggaaacaac
    47401 aacttcaatg gtggtcagcc acctcaaggc aatacagaag gtttattggg agaatacgga
    47461 atcaatatca cagaacaagc acgacaaggt gatatcgatc ctgtgatcgg acgcgatgac
    47521 gaaatcaaac gagtcattga aattttaaat cgccgaacca agaacaatcc tgtcttgatt
    47581 ggagaaccag gtgttggtaa aacagcagtt gttgaaggtt tagcacaaaa aatcgtagat
    47641 ggtgatgtac cgcaaaaatt aatggacaaa gaagtcattc gtttagatgt cgtatcttta
    47701 gtccaaggta ctggtatacg aggacaattt gaagaacgta tgcaaaaatt gattgaagag
    47761 atccgtcaag cagaaaatgt gatcctattc atagatgaag ttcacgaaat cgtcggtgct
    47821 ggttctgctg gtgatggaaa tatggatgct ggtaatattt taaaaccagc tttagctcga
    47881 ggagaattgc aaatggttgg tgctactacc ttaaatgagt accgcatcat cgaaaaagac
    47941 gctgctttag aacgtcgtat gcaacctgta cgggtagatg aaccaactgt cgaagaaacg
    48001 atcagtattt tgaaaggttt gcaaaaacgt tatgaagact atcatcatgt aaaatatact
    48061 gatgaggcca tcgaagcagc cgcaacttta tccaatcgtt acgtccaaga tcgcttcttg
    48121 ccggataaag ccatcgattt gctagatgaa acaggttcta agaaaaactt gacgatccaa
    48181 atcgttgatc ctaaaacaat cgagaaaaaa ctgcaagaag cagaagaaca aaaagtttta
    48241 gcttctcgag aagaagattt cgaaaaagca gcttattatc gcgatcagat caataaactt
    48301 caaaaaatga aagaacgtca gctcactgaa gaggaaacac ctgtgattac cgaaaaagat
    48361 atggaaaaaa tcgttgaaca acggacaggt attccagtgg gtgaattaaa agaaaaagaa
    48421 cagacacaac tgaaaaactt agcagatgac ttgaaagctc atgtcatcgg acaagataac
    48481 gctgtagatc gagtagcgaa agcgattcgc cgaaaccgtg ttggattaaa caaacagaat
    48541 cgtccaatcg gctccttctt atttgttgga ccaactggtg taggtaaaac agagcttgcg
    48601 aaacaacttg cctacgaact attcggttca caagattcaa tgattcgttt cgatatgtct
    48661 gaatatatgg aaaaacatag tgtctctaaa ttgattggtt ctcctccagg ttatgtcgga
    48721 tacgaagaag ctggtcaatt aacagaaaaa gtacggagaa atccttacag tcttgtcttg
    48781 ttagatgaag tagaaaaagc tcatccagat gttttgcata tgttcttgca aatcttagat
    48841 gatggtcgat tgacagatgc acaaggaaga acggtcagct ttaaagatac gatcatcatt
    48901 atgacaagta atgccggaac tggtaaagta gaagcaaacg ttggttttgg tgcggcacgc
    48961 gaaggtgtta ctcgttctgt attgaaccaa ttaaacaact actttacacc agaatttctg
    49021 aaccgttttg acgggatcat cgaattcagt gcattatcaa aagaaaactt gatgacaatc
    49081 gtcacactaa tgctagacga tgtcaaccaa atgctggcag cacagcaact tcacatcgaa
    49141 gttcctacga atgtcaaaga aaaattagtt gacttaggct atgatccatc tatgggtgct
    49201 cgtcctcttc gacgcacgat ccaagaacaa atcgaagatg gtatcgctga attttatctt
    49261 gatcaccctt ctatccatga gttgaaagca aaactggata aagatggaaa aatcatcgtt
    49321 acttctaaac cagaacgttt ggctaaagaa tcagccgaag aaacggctga ataaatccag
    49381 ttttatagcg tgagacggta tttctttgag aaatgccgtc tcattctttt ttcagaaaag
    49441 tcaaaatact gttcatttta taaaaaagaa attataataa gagaaacaag caattaaaaa
    49501 tacataaaag aggtgagcgc catgaaattg gtaaatgtaa caaacagcca ttcccgtctt
    49561 gtattaaacc agcttgaaaa cactgatgcc catctagtca aggtctacac agccggaaat
    49621 accactattg tctacactga agcccctgaa cataatgaaa ttctgctgat caatgacaaa
    49681 cgtaaaatcc agccgaaaga aatcgaagat gctaaaaatt atttcttaga taaaatcaaa
    49741 gacgccgttt accacgagga tgaaatcaaa gtaatcgaat tggcaggcct tgttgaaatc
    49801 tcgattccaa aacatgaatt aggcgctaca ttagccgtgt aaacaaattt tgaacataat
    49861 aattaaagag cgtgggacaa aagtaaagaa cacttttgtc tcacgctcta aacacgtata
    49921 aacggcgaaa gcagaagcaa ctccttcgga aataagctga aattcacgaa aattgctttt
    49981 caaatttttg tgaattccag cttatttctt ggagttaaac gcttctgtct cagcctcttt
    50041 ttctttctag ctttttgatt tttctctttt cttttgtact tttctctttt cattttcagt
    50101 tctacgcgtg tctttatttt taactttttc ctctttatgg atgattgatc gtactttttg
    50161 tgcaatttgc tcgtttttgt cccagtcttt tacatctaat aatcgacata atcgtaacat
    50221 ctcttctttt tctgtttttt tctttcacgt ctttgccatt ctgtttccat gtgtcacact
    50281 ctcctttctt taatcatatg tacgcaaaat aatttttata tttttttgat tagacatttc
    50341 tatgatttta atatagtaaa aagagccgtc acggaaaatc cgtcacggct ctaaacaaaa
    50401 tatgagaggc tgtttttgtt tggctattct tattacttga tgcagggcag cttgtgtcaa
    50461 gacttcttta catcaaactt tttaatttta cattgggata tttatccgca aaccagcgtt
    50521 ctgcaaattg gttctcgaat aagaacaacg gttggtcgaa acggtcacga gccaagatat
    50581 tgcgacttga actcatcttc tcatccaaat cttctggatc gatccagcga gcgattttgt
    50641 gtcccattgg agacatgatt acttcggcgt tatactcatt taacatacga tattggaata
    50701 cttcgaattg tagctgacca acagctccaa tgatatattc ttccgttaga tatgttttat
    50761 aaagctgaat cgctccttct tgaactaatt gatagattcc tttgtggaag gatttttgtt
    50821 tcatgacatt ttttgcagtc actttcataa acagctctgg agtaaatgaa gggagttctt
    50881 cataagcaac ttttagtttt ccttcgaaca atgtatcccc aatctgataa ttgcctgtat
    50941 catatacacc gatgatatcg cctgctactg ctttttcgac gttttctctc gaatctgcca
    51001 taaattgtgt gacattgctt aatttcagtt ttttatttgt acgttccaac cagacatcca
    51061 ttcctcgttc gaatgttcca gaacagattc ggacaaaagc gattcggtca cgatgggctg
    51121 gattcatatt tgcttggatc ttgaatacga aacctgaaaa ttcttcctca taaggactta
    51181 ccatctgatc atcttgtgtt ttgtgtccat aaggagctgg tgccaattca acgaaagtct
    51241 ctaaaaaagt ctgtacaccg aagttagtca atgcagaacc aaaaaatact ggtgtctgat
    51301 cgccacgagc aattttttct tgattaaatg catctccagc ctctttgatt agttctactt
    51361 cttctaagac ttgttcatat aaggaatttt cagttagagg atgattttcg ggaagcagtc
    51421 catcttcatc caattctact aatcgttcat cctgataatt ttccggacga taaaactcta
    51481 cacgattatg gtagatatca taaagacctt ctagcccttt acccataccg attggccaat
    51541 tcatcggata agattcgata tctaatagct cttctaattc ttctaacaat tccaatggtt
    51601 cacgaccatc acgatctagt ttgttgataa aagtaaatat cgggatgcca cgttttttta
    51661 caacttggaa taatttcttt gtctgtgctt cgatcccttt tgcactatcg attaccatga
    51721 ccgcactatc cacggccatc aaagtacgat aagtatcttc agaaaaatct tcatgtcctg
    51781 gtgtatccaa gatatttact cgtttccctt gataatcaaa ctgcataaca gaactagtaa
    51841 cagaaatccc acgttgtttc tcgatttcca tccagtctga tttggcaaaa ttacctgttt
    51901 ttttgccttt tacggtcccg gcttgacgga tagccccacc aaacaatagt aattgttctg
    51961 taatcgttgt tttcccagca tccggatggg aaatgatcgc aaaagttctg cgattgtcca
    52021 cttgttgctt taattttgga ttattcataa cctaactcca tttcttccat tcaattattt
    52081 atattttctc taggcacaga ttgtccgatc tcttaaagaa agacaatttc atatagggct
    52141 gtgacaaaag ccgtgtagca gtctcttttt ccgagtaaac ggcgttctag agacagccta
    52201 tcagcaataa gcccacccaa gccaaaaata cgaaaagctg tttttgcttg gctgctctta
    52261 ttacttaatg caggatagct ttttcacaac ctttgtcaac tactacaact gaatcagtat
    52321 agcatggatt cagcgaatga aaaagccacc aaaccataag agtcaaatat ttttttgtca
    52381 gctgactaaa agaaaagacg tgttgcttgt ttattcaagc aacacgtctt tcataaccaa
    52441 gtttccaatt atcttcttgg ctcgtcatct tcgaattctt ttctgaagta tcttcgttct
    52501 tcttcaggtt ccgcttctgt ttcttccgga tcatgaaaaa tgacacggat tttaaggatt
    52561 cgagaacctt ccatttcttc agaaaccagc gtaatattat cgacatcaaa cgataacttt
    52621 tcgccttcat ccggaatcat gcctaatgct gtaatcaaat aaccagccat cgtatctaca
    52681 tcactcatat gcagatcact gtcaaaagct tcattgaact catcaatcag catacgtcct
    52741 tggatgatat attcgtgttc atcgattttt tcataaagat tctcaacttc atctgattca
    52801 tcatcgattt ctccgacgat ttcttctaaa aggtcttcta gtgttgctaa cccaacgacg
    52861 ccgccatatt catctaataa aatagccatc tgattctgcg tctttttcat ttcatataac
    52921 agatcatcaa taaagaccgt ttcaggaacg aataaaggct cctgcatgat tttcttgata
    52981 tctaaattat caaagccgaa tttatgggca gcttttagta aattctttgt atgaaggatt
    53041 ccgactactt tatctttgtc ttcgttataa acagggattc ttgaatagtt ttcggataaa
    53101 acttcattca cattttcttc cacagtgtcg ttgatatcaa tcataaatgc atccgttcgt
    53161 gggaccatga cttctcgggc aactttcgta tcaagagaaa aaaccccttg gagcatttct
    53221 aattcttcat tattcaatac gccttcgttt tcaagcatat aacgcatttc atctctggtc
    53281 atttttgaat cttcatcatc aaaagtcatc ggcgtgatac gcgccaataa acttgtggaa
    53341 gcagaaagca gccagacgaa gggcttagct actacaccaa tcaagcgtat cagccctgaa
    53401 gtgaactttg cgacttcttc tgatttattt aaagcaatac gtttagggta caattctcca
    53461 aaaacaatcg atacataggt caaaatagcc agcacaatga tatttgcaat attttttgcc
    53521 gctgtgccgc cacctaatac cggagctaac cgttccgata aagtattagc tagtgaagca
    53581 ccagacaaaa tgttgaccaa agtgattcct acttggattg tcgataggaa attatttgga
    53641 ttctgcaata tttttaaaag cttttgagat ttcacatctc cttcatcagc tttttgctcg
    53701 attcgattct tattgatcga aacaacagca atttcggaag ctgctaaaaa agcattgatc
    53761 aatgtcaaaa cgatcaataa taaaatctgc gctaacagcg actgactctc agggtcagca
    53821 ttcataatcg cattctctcc ttataaaatt aatttttagt ttacgataga aaaaaacgtg
    53881 gcactatagc cacgttaaaa tggtatcaca aagcaatttt ctttgcaata aaaacaagtg
    53941 tactaaaact acctacgagg tcgtagcaat tttcttcttg ttttcatttt gcttgtcttc
    54001 tttgacgatt ttgattatat aggaaatgat cgtcttgcag attgcgtaaa aaggaacacc
    54061 taagaagata ccaagcaatc cggcaatatt tccagctact agaagaacga taatgatcgt
    54121 taatggatgg attttcagtg atttcccaat tacattagga taaatgatat tcccatccaa
    54181 ctgttggacg attagcacaa ccacacaaca aagtaatgct tttaccggat cattgaagac
    54241 tgttactaga aatgcaggtg ctagtcctag ataaggtcct aaatatggaa ttaggtttgt
    54301 caatcccgca atgaccccaa ataagaaagc ataatccaca ccaattacca tatatcctaa
    54361 aaatgtgaag gtaccaacga ataaacattc gattgcttgt ccgctaatgt aattagccaa
    54421 tgttttattc agttgcccca ataactccac aatttggttt cttctttttt ctgggaaaaa
    54481 ctgttggatg tttggcacta gtttttctcc gtctttcaac atatagaaca agatgaatgg
    54541 tgctgtaatg attactacgg ttgccgaagc gatcgttcca acgatcgaac ctaaactatt
    54601 ggatactcca ctaaggaatt gctgaatgat tgtcccgtaa gaaatgtcta attgctcaaa
    54661 atatttattg atatctactt ccttgaacaa aggcaattcg gctacctctt ttaaccagct
    54721 ttctacctgt ttgatgaaat caggcatatt agatgccaat gaggaaattt gctgtactag
    54781 acttgggatg acactaagta aaatccaaat aattgctgca actaaaagga gcagcacaat
    54841 tgcgacagcc catattcttt tgatttttgc tttcatcaac agcttgacaa taggattcaa
    54901 taaatagtaa agaaagcctg caatcagtac aggtgcaaat aacgtggaaa aaaatgtccc
    54961 gatcggttcg aaaataaagt ttatttctga tgaaatcatg atcaatgcag ctatgatcaa
    55021 taattcaagc gaccaaaaca ttagtttaga ctgtctaagt ttttcaaaca atagatttcc
    55081 tcctttcttt cttactagta tatattatat tcgttaaaaa ttcttttatt ctttttttat
    55141 ttttgaaaac ttgccatact ttttatttgt tttattttcc agtatactat acgaaaggaa
    55201 ggtggctaga ccatgaaagt tgtacaaaca aaagatacaa tgagcaatat ttacttagat
    55261 gctgtacgga tcaggcatca agtttttgta gtcgaacagg gtgtgccttt gtctcgagaa
    55321 atagacaaag atgaagccca ttgtatccac ttcgtcctgt actcagataa aaaagaaccg
    55381 caaggaactg tacgtctttt gcctttagaa aacggaaaaa tgaaattaca gcggatggcg
    55441 attctttctg aatacagaca ccaagggtta ggaaaaatat tgattgaaga agcagaaaac
    55501 tttgctaaga atcaaggcta taatacgatt ttattagggg cacagtccac agctgaaact
    55561 ttttacgaaa aactaggata tacagcttac ggcgatcctt ttgaagatgc tggcatgcct
    55621 catatttata tgaaaaagac tttataaaaa tttatttcga aaaaagcaaa aaacagcaag
    55681 cttttgagaa aaatctctca ctggcttact gttctttttt gtgtttactt acgcgacaat
    55741 tgcttgtaac ggttatacca aatatcaata tagccttccg agaacgggcc atctccatta
    55801 ttgatccagt caaccagaat cttcacattt tcttttagga tgaagtcaat atcgttcggg
    55861 taattcatca ttttgctgtg catctcatat tcatcgacat ccaatagtcg tttctcgccg
    55921 tcaggaaata ccttgatatc caagtcatag tcgatatatt tcaaggcctc atcgtccagc
    55981 aaaaaaggtg aagctagatt acaataatag gaaaccccct tttctctgat cattgctatt
    56041 acattgaacc agtatttttt atgaaaataa acaatcgctg gttcgcgcgt tacccatcgg
    56101 cgtccatcag attcagtgac taatgtgtga tcattcacgc cgatcataga gtgttcgctt
    56161 gtttttaata ccatggtatc tcgccacgtt cgatgcaatt tgccgtcgtg cttgtaactt
    56221 tggatcgtca caaactctcc ttcttttgga actcccatag caacccttcc ttaagcagca
    56281 ttttgtgctt tgtgtttggg tgttcattac accgacatca taattattat atcaataaaa
    56341 atgagctttg cctagaaagg attcctattg tttcgatttt tctctaactg atctaccagt
    56401 ttcatttgga ccttaggaaa aacgaccgaa tcaaatgcat caggcttcaa ccacttcgaa
    56461 gttttgttct cggtaaattc ttgttctgct ccctcggttg cccgtccgta aaaaaggaga
    56521 acatgccatt tcagatgact aaaaatatga gtaacttctc ctaaatgtcg tgtctgccaa
    56581 acaaagagtt cctgcttttc aaatgggaga ttttgctctt cttctgctac taggtcatca
    56641 aacagatcga tttctggctt tgtttcccat tcttttttca atcgctcata ctcctcttgt
    56701 gtcacttcca tcattggaaa tgtccacata ttcgccagca atttttggct atctctttct
    56761 tcaaaataat acgctccaga atgattttga agagctgcac tgatataata aacgtctttt
    56821 ggctttgctt tttttgtttt tacaggaaaa ctcgtttgga ttcctcgttt gttagctaaa
    56881 caaaaggctt ggatcggaca agtctcacac tttggagaag ttggtgtaca gatagctgaa
    56941 cctagatcca tcattgcttg gttgaattca cctggatgct tttcatcaat gattttccgc
    57001 atcgcttcat caaagatttt cctgctagaa gcttttgcga tgtctgcttc aatgcaaaac
    57061 aatctgctga ctactcgcat cacgttgccg tcaactgcag gttctggaag tccaaatgcg
    57121 atacttgcaa tcgcgcctgt cgtatatggt cctattccct tcaatgagct aatttcttca
    57181 ggcgtttgcg gcatttcccc atcaaattct gacatgatct gtttagcagc agcttggatg
    57241 ttccgggctc ttgagtaata accaaggccc tcccatgctt ttaaaagctt ttcttctgga
    57301 gcatttgcta gctcttcaat tgtaggaaac cattccatga aacgataaaa ataatcgatc
    57361 actgtgtcta ctctggtttg ctgaagcatg atctcagaga tccagatacg ataaggatca
    57421 cgattatagc gccaaggcag gtttcttttt tcttgttcat accattgaat aaactgatct
    57481 tgaaattctt ttgtttcttt atctgtccat ttttcccatt ctttattcac tttcctcttc
    57541 cttttccgta atgaatcgct ggatcatatc caaatcaaat cctttttgat ataagctttg
    57601 ctttactttt agatttcttt ttttaggttc aaaccttaaa tttcttcgcc atagtttatc
    57661 tccttgtttg actaaataat catattctat ttcttgatct gcttcttgcg gaagatcagc
    57721 tatcgcttgt tggatcactt cttgagaaaa gcctttcgtc atcaatgttt gttggatttt
    57781 tctcaataat tctttttggc tttttccgtg attttttcca aacgcttttt cggctgtctg
    57841 atgtgcattt tgaacttggt cttcaaatgt atattggctg agcgcttgtt caatgatttc
    57901 aggtttcaaa cctttttgct gcatctgctg cgctaatttc tttggtcctt tgtcacttaa
    57961 gcgcatattc gtgcgcacat aactttcccc atagaccaaa tcatcgacaa ggtccaattc
    58021 ttttaagcgg gctacgattt tatttcgatc ttcttgaagg atttcctttt cttttaagta
    58081 tgttcggatt tctttttccg atcgcaactg ataacttaag taattcattg ctaggtgaaa
    58141 accgaaatca gctccgctac tcttcttgat ctcttggata gtttcttcgt ctaattcccg
    58201 tccttttaat aatcgatagc gtactaatac atcttcagat acttgtaaag cttctccttc
    58261 agataaatct acgcgataga aagggccttt gcctttactg attctcgtaa cggttaacat
    58321 aaacatcctc ctcgaacata tattctctat cataccacag aaaatccccg taatcaggta
    58381 ttatatgtta gaataaacca cagaaaaaag aaaaagggag atacttatgg cagaaacaat
    58441 tgtcaaaact ggacaaacgg ttcctttgaa aatcaagcgt ttaggcataa atggtgaagg
    58501 aatcggttat ttcaaacggt tgatcatatt tgttccatat gcactaccaa aagaagaagt
    58561 actagtgaaa atcacgaaag caactcctcg ctatgcagaa ggagaattaa taaaagtcaa
    58621 aaaaacaagc aaagatcgcg tagtggcacc atgtcctgtt tactatgaat gcggtggctg
    58681 tcaattacaa cacttagctt atcaagctca attggatttt aaaaaggatc tgcttttgca
    58741 agcattggaa aaattcaaac cagcaggctt tcgatcttat caacttttgc cgacgatcgg
    58801 gatggatgag ccttggcatt atagaaacaa agcacaattt cagttacgaa aaaataagca
    58861 gactcaaaaa attgaagctg gtttatacga agcaaactct catcagttag tccctataac
    58921 agactgcttg gttcaagaac ctcgaacaca aaaagtaatg aatactgttg ttcaactatt
    58981 gaataaattt gatgcaccca tctatgatga acggacaaac agtggaatct tccgcacaat
    59041 catggttaga atcggtgtta aaactgggga gttacaagtt gtttttatta ctcgcagtaa
    59101 aaaattcccg caaaaaaacg caatgatccg tgagatcaat gaacgactgc ccgaagttgt
    59161 ttcaatcatg caaaatgtcc aggcacaaaa aacgtctctc gtgatggggg atgaaacgat
    59221 ccacttatgg ggaaaagaag ccattgaaga acaaatcaat gaagtgactt tcgatctttc
    59281 tcctcgtgcc ttctttcaac tcaatcctca gcagaccgaa ttgctttatg gcgaaggaat
    59341 caaagcatta gatgtccagc ctaatgaaac gatcgtggat gcttattgcg gagttgggac
    59401 gatcggctta agtgtagcaa aacaagccaa agaagtacgg ggaatggata ccgttcctca
    59461 agcaatcgct gatgcaaaaa aaaatgcgga aagattagga tatcacaaca ctcgatatga
    59521 agtcgggaca gcagagcaag ttctgcctaa atggttgaat gaaggttttc agcctgatgg
    59581 tatcatcgta gatccaccac gtacgggttt ggataacgca ttgatccaat cattattgaa
    59641 ataccctcca aaaaaacttg tttatatctc ctgtaatgtt tctacacttg ccagagatct
    59701 ggttaattta agtaaaaagt tcaagattga atatttacag tccgtcgaca tgtttcctca
    59761 gactgcacgt tgtgaagttg tcgtgaagat gacgagactc gctgactaaa tgaaagaggt
    59821 tgtgaaaaag tctgccctgt atcaagtaat aagaacagcc aaacaaaaat agctcttcat
    59881 gttttatgaa tactgtactt gtgaccgaag agttaacagg aggacactat ttatttaaaa
    59941 aaagggttgt gacatagttt ttgtcacagc cctttttgat tattattgga tattcacgtc
    60001 tatcacttga taaaatgcat ttgtcgtatc gctgatattc catactgcta aaatcacatt
    60061 atatcctttt cgatcctgcg gaagtgtgat cgtttggttg actgtttttg gtggaacagt
    60121 tgctttgtcg tctatttttg tgatcaattc aaaatcacta aatttcaaag gttgattagg
    60181 attccaagtt ggtttggtaa tgtagtaatc ccaagtactt gtccgatgtt gtgctgttaa
    60241 attccaagta atatttaatg ttcctggatt aaccatgttt ttatgccatc tgttagatgt
    60301 ctgttcatct aaaggctcaa agccgctgac tcctgcacta gcaatttttc catcaatgaa
    60361 tgtatttttg gtagcttcga tactttgcgg ttcccattgt gctcttccga cattttgatt
    60421 taagtttcct gctgctgatg ttcctaggta tgcacggctt cctggagaag taatataacc
    60481 atgcgcaaaa cctggaatgc taaaaccgaa caaccctaaa ctaactaacc caataaacaa
    60541 taacgtacta atctttttca tgtagaaacc tccttcattt gataaccaca ttgtaaacgc
    60601 tttattgtat taacacaaac attatgcttt tataatttta tgttgaagta aaaaaggtga
    60661 aaaaagtaag atgcagatta taacagaagt agacgagaaa agtacaaata gaaaacatga
    60721 gaatcaaatc atatgatctt tttcaaaaac atcattcata taaacaaatg ttcaactttt
    60781 taataacaac aattatcaat ttttttctta cttcacaaac taaatacagg tcttgctgtg
    60841 agccttcatg gtaaactaat attatagaaa ggagttagta aaatgaagat gataaaagat
    60901 attcgttttt gggtttgtgt tataggaatt gtcatattag gctttttatc agggttgctt
    60961 agcggaaatc ccggaaaata ttactattct ttgcaacttc caccatttgc accacctagc
    61021 tggatatttg ggccgatgtg gaccttactg tatatattga tgggtatttc tctttatttg
    61081 ttacttaatc acaacaacaa aaaacagcga aacaatcttg tcggtctttt tgtgattcag
    61141 ttcattttta attttatatg gtctgctcta ttttttaatt taagaaatat tttcattgca
    61201 gcaatcgata ttacactttt agttattttc ttgtcagtgc ttatgtatca gttgtggctg
    61261 catcatcgcc tgacaatgtg gctcatgatt ccttattatt tatgggtact attcgcaact
    61321 ctattgaatt attcaattta ttttttaaac taagcgctaa aaagagacgt cctgtaattt
    61381 aacacataga aaaagtgcca cctaagttag attttttgtc taatttaggt ggcactcttt
    61441 attaaccaaa ctattttttt ctagtaccca cttgttgttc catactcatt attctggtca
    61501 taaccataac cattttgttc ctgtccagcg taaggatcat aactttgagt atcttgactt
    61561 ggtgtatagt actgacttgt atcttctgtt tgctgataat aggtattcgg atcttggtac
    61621 tgatcatatg tgttttctga ataggaagaa taatcggtgt acccttcact attcgatcca
    61681 tcaccgtttg tcgaatcgcc atcttccgaa tcatcaaaga acatcattga agcattctca
    61741 tttttcaaat taggtaatgt atcgcttgtt tttatattta attgtttctt cagttcattt
    61801 tggatcgaaa gaagatcatt ttttcctaaa atttgatagt agatatcatt gatcatttgt
    61861 ccttcgcctt gcaactggtc ttgtttgatt gtttcaaacg ccggggtgta gttcgtcgct
    61921 acatcaagca tgtcatccca atctaagtcc gtcgtcacat tgttctcaac tgcttttaaa
    61981 atttttctat aattgcttac gccatctaaa cttaaaactt tattgacgat tttcgtcact
    62041 acttcccgct gacgacgttg tcgaccgata tcgccttctg gatcttcgtg tcgcattcga
    62101 gaataggcta aacctttttc tccattcaat gtttgttcac ctactggtac atggacacca
    62161 tccaaagtaa agtcaatttt attattcact ttgatgccac cgaccgcatt gatcaaatct
    62221 tccatgccat ccaaattgat cgttacataa tgatcaatag gaatatcgag cagttgctca
    62281 attgaatcca tggccatttc gacaccacca aatgcgtagg catgatttaa tttatctacc
    62341 gttccataac ctacgatatt cgtataaata tctcggtcca aactgatgat cgttgatttt
    62401 ttttgtttcg gattaactgt tactaccatc atcgtatcag aacgtccttg ttccgttctt
    62461 cctaatccac cggtatctaa acctaatagc aaaaccgaaa aaggttcttt gtcatcaatg
    62521 cttactctct cagaacgttt ggaggatatt cgattagagt ttttagaaat tttgtttact
    62581 gtttggctcg cttctcccat aaatctgatt ccgtatgctg ataatccggc aatcagcaaa
    62641 acgaataccc ctaacaaggt taatagaact ttttgccatc gcttcataat acacctctgt
    62701 ttcacatttt ctaaaatatt gtctcatata actgttcaat cagcaaattc actttttaat
    62761 cataagaaaa ctttaaaaaa catttttgtg taacgattat tattttttaa aatttctcaa
    62821 aattcttaac tatcatacca taccgtgaaa aagatcgaaa gaccgaatac acgcagttcg
    62881 caatcgtttt atcttttttt atgtattttt tccagaaaaa gcgaacaaca aggtattttc
    62941 caaataatcg tgcagtaaaa tgatcgcaca tacctttccc atctttttaa cagtctttgc
    63001 ctaccgttca gattatcatt cgatttcacg acaatttctt cctatgactg gtccttttgt
    63061 taaacccatc ttctattcgt tttatttcca gctcactgca tggctcttaa aatgactcga
    63121 actagtgatt gcattaccat catcttttaa tggatagata tgctcctctt caaagtaaaa
    63181 cgattttctt ttaaagtaat agaattgaca tggtaatgag tatgtatact agtggtttca
    63241 caaatggtct tttattcaac atccaaaaac tgaaatttct ttaaaatcca ttactaacga
    63301 agaaaatgtt ccttgcttga taattagtta ggacatgtca agtgaacaaa tgcatttact
    63361 tagccaaaaa atctaaaaac ttcataaata gacaaaaaga cacgactctc taagaagtcg
    63421 tgtctttatt caacaaatta ttataattta gtcacttcag cagcttgagg tccgcgtgca
    63481 ccttcaacaa tcgtgaattc tacttcttga ccttcttcta ggctcttgaa gccgtctcct
    63541 tgaattgctg agaaatgaac aaatacgtca tctccgccgt ctactgtaat aaacccaaat
    63601 cctttttcat tgttgaacca tttaaccgtt ccgtgttcca taaatgtata tcctccaaaa
    63661 aagttcttgc aagtaacctt gcaagaacat tatacccaat ttagaaaacg ctagcaagct
    63721 ttcgctcttt tattttccaa attattttta agactgatat gacggaggaa atggttattt
    63781 tgctgcttcg aaacgtttgt ttacttcatc ccaattaata acattccaga atgctgcaat
    63841 ataatctggt cggacatttt tatatttcaa ataataagca tgttcccaaa cgtccaaacc
    63901 tagaattggt gtctttcctt ccatcaatgg agaatcttga tttgcagtag aggtaatagc
    63961 taattttcca ttttccatta caagccaagc ccatccagaa ccaaatcgtc cagcggctgc
    64021 ttttttgaat tcttctttaa aagaagaaaa atcaccaaaa gcttcattaa tcgcttcttt
    64081 tatttctcct gtaggttcgc caccagcatt tggtgccatg atttcccaga aaaatgaatg
    64141 gttagcatgt ccgccaccat tgttacgtac agctgtcttg atatctgttg gaatagcgtc
    64201 catatcagat aataattctt ctattgtttt ttcgcctaat tctgggtatt tctcaattgc
    64261 tgaatttaaa ttcgtcacat aagtattgtg atgcttatca tgatgcagat gcatcgtttc
    64321 ttcgtcaata taaggttcca atgcgtcata tgcataaggt agatctggta atgtgtaagt
    64381 cataaaaaaa ttcctcctca atgttttctt aataatgtct acattacaaa gagtaacaaa
    64441 gagaaggaaa ttcttcaatt gttatgccta gtcgtctttt tcactttgac ctacttcttc
    64501 ttcaaaaacc tcagtttctt taaaaataaa caacttgctg aaaatatagt tcgcaaggat
    64561 aacaataatt tgagtgatga ttttcgccac atactcattg ctgttcataa cattgatcaa
    64621 caataccatt gcccccatgt ccaatccgta agaaaggata cggtagaaga agaacttacc
    64681 gcattcaata agtgcatgtg taaaattcgg tgattttgaa tggaataccc aaagtttgtt
    64741 cgttacaaaa gcaaacaata cagaacaaaa ccacgaaata gaattggata aggccatact
    64801 taaatcaaaa tattggcgtg cgagagcaaa actaacaaaa tttacaatcg tagccaaacc
    64861 accaaaaaag agatagacta gtatctccca atatcctttt gcttctaaat atccttttaa
    64921 ctgtcgatac tgcttcatga gccaacctac cttattaaaa attcttattt atcatactct
    64981 atatttcaat aactttcatc tttatttaaa aaaattaatt ttttttgatc ctttgccttt
    65041 gtctctgctt ggatcgtcac atgtgtaata ccatatttta attttaataa agaaattata
    65101 cgaggatata tcttttcaat ctcgcttagt agctgatctt caaaatcaat atgtccgtcg
    65161 aaaataatta tcttttcgct atactgccaa atatggacat gatgaacatc ctttacccca
    65221 tcgattgctt caatttcttg ttccattttt tcatagtcga tagcaggtgc actttgtatc
    65281 aaaatagcgg cagctttttt gatcaccatt atcgcttcta tcaatatata tatcgaaatg
    65341 atcagtgtaa taactggatc gatccagtag atatcgaaaa aacggatcaa aattgctccg
    65401 ataatcacac ttaccgaaga cagtgtatca cttaacaaat gcaaataact agattttata
    65461 ttcaaacttt catctgcttc ttgtcgtaat aataatactg agaacaaatt cgcgagcaat
    65521 ccaataactg ctactgttag catcaaatga ctatttatct tttctggtga agtgaaccgc
    65581 ttgaatgctt caacggttaa aaacaaggat aatatgatca atatgaatga gttgatgaaa
    65641 gcactcaata tttctaaacg ctggtagcca tacgtacgtt ttttatttgc ttcttttcgg
    65701 gccatttttt gtgccccgta agaaaaaata attgccaatg tatcactaag gttatggaaa
    65761 gcatctgaaa caagagacaa actccctgac ataatccctc caataaattc tgataaagtg
    65821 atcaccgcat tcaaaaccac tactccaaat agtttttttg atgatctctg tggctgtgta
    65881 ggcactattc cttcctccat tttatttata ttggatagtc tttagacaag aataacattt
    65941 ttttgatatt tcaccaatca ttcagctgtt tccttaaagc acttgtattt cacttgatac
    66001 ttcgctagaa ttgcaagtga tgacactact agatgaggag tacttatgac aacaaaacaa
    66061 ttaattatca gttcctttat acgattcaaa gacctaaagg aggcgagaaa tgtcccattt
    66121 tggaaagcca ttgtctatct tttagttttg agtataatta tggctttgcc aatctctttt
    66181 caagtttttc aagtcttaaa caacattcaa ttggatgggc aaaagattgc tactcgcatt
    66241 cctgatttta caatccaaaa cgggcagctt caaaccgaag aaaaagaagg attcatttac
    66301 caaacaaact ccattatctt cacatttgat ccagagggaa aacgaacaga acaggacatc
    66361 tctactgatt tgatggggaa ttttgttagt gtcgggatgt taaaaaacaa acttatcata
    66421 gctcttccta atacaggcac gacttctgct ttgttgaaca ataaccaatt agaacttccc
    66481 tacaccaatg aatctttaaa aaacttaaca ggtaagcagc tccgttcttt tttaagtgaa
    66541 gcatctatac cactttggat aaaagtcctg acatttatat tttctattta tcctagtttt
    66601 ctgaatctgc tgatcacttt gctatttacc aacattgctg cttttctcta tgcacgcttt
    66661 cgactaacaa aagctacttt tcttgattgt ttaaaaacat tgatctattc aatttcattg
    66721 cctactataa ttgcaacaat cttgatggtc tttcttcctt cttttgacac tagcgccttc
    66781 attgcttttg caggaatctt tatttttgct caagctgtca aaggatgggc aaaaatatcg
    66841 atcaattaag aaataaagac aactcgttat ttctacttca atagaccctt ttagaggttg
    66901 tgacattttt ttgtcacagc ccctttttta tttgaaaaat atttctttct caaaaaacaa
    66961 gcaaggcgct tcaaagttct tcttgtatgc gctacactta gagtaaagaa aggcgggaaa
    67021 ttaactatgt cagaaagaat catttatgca gatcgtccat ttcgtgatga atttatcgaa
    67081 gaaatccata ctatagcgcc tggttatatc tttaaaacag aattacaacc agaggatcta
    67141 caaaatgtag agatcagttt gggctggaac tcagactacc aagaaaaact attagcctct
    67201 tctaccttga aatgggtcca atctatctca gctggtgttg atcagctacc ccttgagact
    67261 ttttcaaaga aacatatttt actttcaaat ggtagtggca tccacagtga atcgattacg
    67321 gagcatgtct taggaatcat ccttgggtat tctcgtggtc tttttcaagc acaaaaggca
    67381 cagagtgaca tgaaatggtt aggttcatcg gtccattacc aatctatcca agggaaaaaa
    67441 atactaatca ttggaactgg ccatattgga aaaatgattg caaaaaaatg caaagcatta
    67501 gacatgaact gctatggaat caatacatcc ggtcatccag cagaaggaat ggaaaagact
    67561 ttttctttaa atcaactaaa ggaagttctt cctgaagtag atttcgtagt caatatctta
    67621 cctctaacgg aggaaacaac tgggctcttt gatcagcata tgtttgaaca ctttgatcca
    67681 aaatctgtat tcatcaatgt tggccgtggt gcttctgtta agacacaaga tctggtgcag
    67741 gctttaaata accagcaact ttcctttgca gcattagacg tttttgagga agagcctctt
    67801 ccacaagatc atcctctttg gaaaatggac aatgtactga ttacttccca cattgcaggt
    67861 ttgacacctg attttcaaaa gaaattaatg gctatttttt taaccaatct gaaaagctac
    67921 tttgccaccc atgagcttca aaccaatcaa gtaaaattgt ctgcaggata ttaatagctg
    67981 aaaacaatat tttcaaaaga aaaaagagaa ggtgacaaaa attgtgtcac cctctctttt
    68041 tttattgttt tttaaagtga taggttggct cctccaggtt gggatcataa tcaacaagta
    68101 aatcatatcc tttaaagaac cattcatctg cttcctcgat aaaaaaaagt gtcccatcca
    68161 tttccacttt ctttacagga tgttctggct gttcaactga cattccaatc gaaaaaccat
    68221 catgaacctc tgttttgccg tagacttttc caaaaaaacg tattccgtaa gtttctggca
    68281 gatcaatttc tgagtcaaac cacttttgtg cttttggcgt taccgttaat ttcatcccaa
    68341 caacctactt tctcttttct ttctattgat tcatcttatc gtctagaata tccacatcgc
    68401 ctgtctctcc aataaccagt agattatcat tttcacgaac atattcatct gctgcaggag
    68461 aagcgataac cgtttgatct actcttcgta tggcgactac tgttaagtca aaacgttgcc
    68521 tgaaatttaa ttcagctaat gttttgttga aaaatttagg attggagact cggatttctg
    68581 ctaaagaata tttatctgac aactctaggt aatctaaaat attctttgac actaaattat
    68641 gcgcaatcct tattcccatg tctcgctcgg gatggacgac gcgatcagct ccgatctttt
    68701 caagtactct ggcatgatac tcattttggg cttttgctaa aatatttggt acgcccattt
    68761 cctttaccat cagagtaact aagatactgg cttgaatatc ttctccaata gcgacaatta
    68821 catgatcgaa atttcttatg cctaatgatc gaagtgtcat ttcatcctgt gcatttgcca
    68881 caacagcatg agtggctata ttcatatatt cattgacccg atcttcatta ctatcaatag
    68941 ccagtacttc ttgaccagct tcgatcagtg ttcggcagat acttccgcca aatcgtccaa
    69001 gacctacaat tgcaaaattt tgtttcaccg ttttcctcct ttagtatctt cttttctcag
    69061 tataacaaag cctttacaaa aacaaaagca agcatcatta ttgtaaaaaa gccaagaaaa
    69121 ataatagtta ttttctcagc ttttgattat cctctttatc atcgttttta tttcaccaaa
    69181 ccatgtttga atgcatagat agcggcttgt gtacggtctt ccacttctag cttagccaat
    69241 atattggaaa catgtgtttt tactgttttc agtgtaataa acaattcgtc cgctatttcc
    69301 tgattacttt ttccttcaga aatcagcatt aaaatttcat tttcccgatt cgttagttct
    69361 tcatgtaata caggctcatt tcgccgactc attttttcca tcatttttgt tgtcacttct
    69421 ggttctaata ctcgctcgcc acgttgcgtt gcccttatag catcagcaat ttcatgtgcg
    69481 gtagaggtct tcaacaaata ccctgctgca cctgcttcaa tggcaggata aactttttcg
    69541 tcatcaataa aactggttac gataatgatt ttagcttctg gccaatcttt taagattgct
    69601 tttgttgatt cgataccatc catttcttcc atgactaagt ccattaaaat gacgtccggt
    69661 cgaagtgtca tcgccttctc atagccttgt cgtccatttt cagcttctcc aattacttca
    69721 atgtcttctt gaatagataa ataagaagag acgcctaagc gcaccatttc atggtcatct
    69781 actaataaaa cttttatcat ccttatctat gcctccttca tcaaagggac cttgatttct
    69841 acacttgtac cttgaccttt aaaactaatg atttttactg ttccaccaat accagctact
    69901 cgttctttga tattgttcaa tccataactt cccgttttcg tttcattcat attaaaccct
    69961 gttccgtcgt caataatacg taacaaaaga ttattgtcta ttttgtgcaa atatacctct
    70021 aattcattgg ctttagcatg tcttaatgta tttgataaca attcttgaac gattcggaac
    70081 aagtgatcct caatggaact agatagtttt acatcttcta catcccattt cagcgaaatc
    70141 tggattttat tttgcaattc cttcaataac tgctcgatac cttgctttaa acttttctct
    70201 tctaaattga ctggacgtag atgaagaagc agcgcacgca tctcagactg ggaagcgttg
    70261 atgatctctg ctaccatttt caactgcttg cgaaacattt ctggtgtctc gcttttttct
    70321 gcttgctccg tcaatgccga catcatcatc attgctgcaa acaattgttg cgaaactgaa
    70381 tcatgcagct cacgggctaa tcgatgtctt tctaattcta aaatttcttc tttggtttgt
    70441 ccatctacat attggggacg gctcgttaca atttgtaatt cactagatac ttcaatcatt
    70501 ttttctttta tcttagtgat ttccttatca atatctttga tgtataaatc gtccgatgca
    70561 cttgggatcg gaagaagcaa caatttggat tcataattgc cagaagaaag tgcacgcagc
    70621 ttttcttcga ttttcccgta ttttgtcttt tgaataagtg ataataatag aaagacaatt
    70681 aatcctacac ctaaggaaat ggctaacaaa tggaagataa gcggaacata taataaacgt
    70741 gcacgcagta attctcccca ccaatgactt tgattgctgg cataaaaata agtgaaaagt
    70801 gaaaatagaa taataaggaa agccgcaatc cccgaataaa cagctagcat cgctctggat
    70861 atttttccca tcatattcgt atcacctcga catctccaat caaggtatta gtaataatct
    70921 tcaatctacg agggttttca tcataatcac tgctgtagat tttgatttgt tcatttttca
    70981 aagaaaattg ctcttcttca aacaagacat ttcctactaa tgtcgcatgc tctaatcgga
    71041 tggctacgcc caaaggtact aagatccgtg ttctgcctat cccttttctt acaatcacaa
    71101 tattatcatc ttttggcaaa agcgtattgc ccaaatctat gatagtatca ccagaaatga
    71161 cggcgatgtt gatatcgtcc cattcataga cgtcgtttcc aatacgttgg ttaccgaata
    71221 attgctgtct tttccgctca ttattatgtg tttttaattg ctctgtttgg atcatcatga
    71281 tctgtttctt tcgccaaaaa gcatttttgg ttaaatctat ccctgaaatc tctacacctt
    71341 tcagaccaat aaataaaacg gcaaatacag ccatcatcca taaagcagga ctgttgacta
    71401 agctaaaaaa aatgaccaaa cttcctaata tcaattgaaa attttgaaat ttagtacgag
    71461 gatgtttgcg catagctaga tatatattga aaataccgaa tatcactaaa agcaaaagtc
    71521 cagtattgtt tacgatttgc caaacagcaa ataacagcag tagtgcttca actaccacaa
    71581 aaaaacgcca agaactattc atcgctcttt ctccctcctg acacattctc atttcatcat
    71641 acctattttt ttaactgttg gctataggac tttagatgta agatcgtttg ttgaaatcta
    71701 aaatacacaa agagaaaaag atctagagtg tatcatcagt aagatgaact ctctagatct
    71761 ttcagtcaga ctacgcttgt tctactttga cgattttaac taacatatcg ccacctggag
    71821 tagcaatagt tacttcatca tttttacgtt tgccaatcaa tgcttgcgca ataggagaat
    71881 cattggaaat tttgccagaa aacggatctg cttcagcgct tccgacaatc gtgtactctt
    71941 cttcttctcc atctggtagt tcgatgaatg taactgtttt accaattgat acctcatctg
    72001 catctacacc gtcgttatcg atgatttgag caaaacgaat catattttca attgttgtga
    72061 ttcttccttc aacaaatgct tgttcatctt tcgctgattc atattcagag ttttcggaaa
    72121 gatctccgaa accgcgggca attttgattc gttcaatgat ttctccgcgt ttgactgttt
    72181 ttagttcttc taattctttt tctaattttt ctttaccttc tagagtcatt ggataaactt
    72241 tctctaccat gtgtaacgtc tccttttttc gataaaatac cgcaaaaggt aatcttttga
    72301 ggaatatagt ccaaaaaaga ttaccacgct tttcttcaaa tgtaaatgtt tttctaaaaa
    72361 tgatcaatgt ctagagcctg tttttctaag aattatttac atatttttcc actaattcca
    72421 tatgttcttc ataagtttta gcgaaataaa catttccagt gctcgtatct gctacgaaat
    72481 aataatagtc attttctgct ggttctaata ctgccttgat tgcctcttcg cttggattgt
    72541 caaaaggtcc aggtccatag ccggtattgg tatacaaatt atatggtgaa tctacagctg
    72601 tatcttcata cgtcactaat tctttgtgtt cacctaatgc gtataaaaca gagatatctg
    72661 actgaagtgg catatttgct ttgattcggt taaagaagac ttgtgcaatg tttttacgat
    72721 cattttcttt tacgccttct ttttcaacta gagaagctag agttaaaact tcttgtacag
    72781 tcaaattttt ctgcttgata gctgaataat agttttgcat gaccgtattc gtcttgttca
    72841 ccatttcaat cactagatcc ttaagcgaag tactcgtgta ataatcatat gtggctggga
    72901 ataagtaacc ttccaaacgg tattttgtgt tctcagcttt agatgcactt tccaacagtt
    72961 ctgggaattt ttggtgcaac tcgttaaaga aagtctcatc attcactaaa tctaaaaatt
    73021 ctttcttgtc tttaccagtt accttcgcta cccgttcagc aatctgatcg atatcgtacc
    73081 cttctggaat ggcaattttc ccatctgcaa cttttgttgg ctcagaagta ccgccttctt
    73141 gcaactgttt gccaatctcg tcaagagtca tattaggcgc taactgataa tagccagctt
    73201 gaaagccagt taaattttta aattttgtat aataattgaa aacaatcccg cttttaataa
    73261 tattgtcctt ctccaaaatc tcaccaatct gcttgttaga agagccactc gggatctcga
    73321 cttggactaa ttggtcatct gatttatcta aaggcttcaa cccgctatct acataacgat
    73381 acacggtaaa tccaagaaat ccgccaataa tcaataatgt aagagcaatc actaaaatga
    73441 tcttcctcac aatcttatct tcttttttac gcagatttct ccgcttgtcg attatatctg
    73501 ctgtagctgc cttactggct tcttttttat ctttacttgg gtgttgtttg tttcctgaca
    73561 aaacaactcc tccttactat caagttcgta actttattca ttatacatga tatttgaatt
    73621 ggcgtgaatg attttattta aatattctat caaaaatgta agtagtaaat ccattactat
    73681 aagattaaaa taaatgttat caagatacaa gctattttag ctttattctt tctgtaaaaa
    73741 agaaaggaga ctaaataaac tattatgaaa aaaggaaaac atgctaaaaa aagaaaatat
    73801 tcatttttaa tgatcgttgt cgtgttgttt agccttagtg cgttctctac aggaactatt
    73861 cttggatggc aattttacaa taatgaacac tctcagatag ttgcccatgc tcaatcagct
    73921 caaaaaaagt tgatactgac taccaatgaa caacagctcg ttgaaaaaat cgatcaacta
    73981 ttaagaaaaa atgattatat tggctcaatt tacgtaaaga aaaataatcg catcatcatg
    74041 gaaaaaggct atggctatgc gaataaactc acgcaacaac caaatgatcc ttctctttat
    74101 tatcagattg gttcggttca aaaagcattg actactcttt tgattttaaa acaagtcgag
    74161 cgtgggcacc tttctttaga tactaagctg gctcaattct atccacaaat tgctggcagc
    74221 cagcaaatta ctatccgtga cttgctgtat atgcgctcag gcttgcaccg aactgcttct
    74281 ccaaccatac cgatgactga tgaagaagtc gttcaatttg cgattcagca cttaaaattt
    74341 acagattatc agacctctca ttatgattca ttgaacttta ccatattgac tggaattttg
    74401 atcaaattaa caaatcaacc gtatgaaaaa ttattgaccg atgatatcat cgaaccttta
    74461 ggattacagc atacaaaatt ttatgaccaa gtcaaaaatt catccaatca tgcactatcc
    74521 tatcaaatgt ctaaagagga tgattacttt aaaccactga cagaatctga aacagagatc
    74581 cataacgaat tagggactgg taatatcagt atgtccgtct atgatttaaa tacgctgttt
    74641 acacaagttt tatctggaaa actagtttcc aaagacttac tcttctcatt atggcgtgaa
    74701 aatgcgaatg gacatccata tagtggtggg gtatatagcg gagataatta tatcctgtcg
    74761 caaggaaata tcgactcttt tgatacatct gctatattta gcaaagattc acaggatgct
    74821 atcatcatgg aaagcaatat aaaagctgac aaaactatca aactgcctac tgctgatcta
    74881 agaaaccaaa tctatgacct gatggaagga actactaagt tggcaaatag ctgatatgat
    74941 gaaaagatag caaatactac ttctttgaat agcatttgct taaaaaaagt actcgactaa
    75001 acgtaggaaa gagtgtggga caaatctaaa aatcaatttt tgctacagac tttaattcca
    75061 aataaacaga acagaaagag gtgttcttct ttctgttctg tccaaattat tctaagattt
    75121 ctctttacca acattatttg acaaagggcc tattaaatct aaaatagtat ctttagattg
    75181 ctaaaatatg tgctagcaaa tcattcatgg cttgttctta tttttaattt tatgcaataa
    75241 aaaagaagaa caattagcgt gattatagaa ataaataatg agattttaga aaaaaatgtg
    75301 tatgaatacc tgattgttac tttatttatt ccactttcac tttttactgt aacaaaagga
    75361 cctccttgtt ttagtactgt agcatttttt gaattaacag taaccgttaa tcctttatac
    75421 cggaatacag gtaaaactat tgttttgcct tttgaagatt taaacgtaaa tgtaatttca
    75481 tctttatttg gagaaaagct actataaata acttgaccat cttctgtaac ttgatggttt
    75541 ttcaaaagca attgatcttt tttatagcct ataggtacat aatcaaaatt ctcaaattct
    75601 tcaagtttat tttccatttg ttcattagaa taagttcctt gaaaattcat ttctttcacc
    75661 atgttaacta ctgatgaata atttaaaatt gaaatagcta atattaataa tagtataaaa
    75721 tttcttctcc ttgtataaga aaatttttcc aactgtggag aaataaatag agatactgaa
    75781 aaagatatga acaatgttga aaatatcata aaacgccaag gagcttgaat aatatttaaa
    75841 ggagtatttt gtaaggcaaa ccatggaaat atttttgtac aaagtagaat agatattata
    75901 cccattatat aagtgatttt agataagatt cctaattttt taaagttaaa tgttagtata
    75961 attaaacaaa taagcaaagt taagccaata ccaaatgata gtaattctga atctatactt
    76021 ccaatcaaaa actctttacc cagataaatt gctctttttt gtaatatatc cacataaggt
    76081 ctattcagat cttgatacaa tgtctgctca atcataggac ctataaacgc tagtgaaaga
    76141 agtaaagtaa ctatagcagc ttttattaat ttaataatta agaagatttc tattttcttt
    76201 ttccgataaa attgaataat taaagcaatt ataatcatta ccatagtcat agctacagaa
    76261 agaacatgag tgtaaattaa taaagtcatg ctaatcgcca acataaccca agattctttt
    76321 tttgagtcgt aaagctttat aaacccgagt aaaataagag gaagtattga cattgcgagt
    76381 aattccccca ttgcccctcg aagaaatatt tctacactcc taaaagaaga tgttgtatat
    76441 ataatagcga aaaaggagct agtaacatgt tttttggtaa tttcatacat tacataatga
    76501 catataaata gagtaatgag cgtaacaata taaagaaaaa ggttatagcc tattgctaaa
    76561 ttagtaaact tgatcaataa aaataaagga tatagagtta accatgggta aaaattattt
    76621 acttgcatgc catatgatct aaacgtttca aaattaatag gggagctaaa aatagattct
    76681 aaacttaacg cacgattaat gtgaaatgcc gtatcataaa agccaaatat ttccatgttt
    76741 ttaaatgctg gaaataaata taataaacta acaaatgcat aaactataaa tatttttaca
    76801 gaaaaaattt ttttcaaatt catataccac ctcatgtttt tttatattac atatagtatc
    76861 atttattttt ttaaaacact agattagtca ctatataaaa tgtgattaat tttattatta
    76921 ataaaatatt ttttatatta caaaagtagt gtttcaaagt tagtagagaa aatgta
//
LOCUS       DACDYI010000003        67252 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.003, whole genome shotgun sequence.
ACCESSION   DACDYI010000003 DACDYI010000000
VERSION     DACDYI010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 67252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.003"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            35..466
                     /gene="mraZ"
                     /locus_tag="IWM52_000149"
     CDS             35..466
                     /gene="mraZ"
                     /locus_tag="IWM52_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAP9972436.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            487..1446
                     /gene="rsmH"
                     /locus_tag="IWM52_000150"
     CDS             487..1446
                     /gene="rsmH"
                     /locus_tag="IWM52_000150"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAP9972437.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            1451..1852
                     /gene="ftsL"
                     /locus_tag="IWM52_000151"
     CDS             1451..1852
                     /gene="ftsL"
                     /locus_tag="IWM52_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAP9972438.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            1853..4045
                     /locus_tag="IWM52_000152"
     CDS             1853..4045
                     /locus_tag="IWM52_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP9972439.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKPMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            4071..5033
                     /locus_tag="IWM52_000153"
     CDS             4071..5033
                     /locus_tag="IWM52_000153"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAP9972440.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            5047..6408
                     /locus_tag="IWM52_000154"
     CDS             5047..6408
                     /locus_tag="IWM52_000154"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAP9972441.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            6434..7522
                     /gene="murG"
                     /locus_tag="IWM52_000155"
     CDS             6434..7522
                     /gene="murG"
                     /locus_tag="IWM52_000155"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAP9972442.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            7537..8757
                     /locus_tag="IWM52_000156"
     CDS             7537..8757
                     /locus_tag="IWM52_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAP9972443.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            8913..10238
                     /gene="ftsA"
                     /locus_tag="IWM52_000157"
     CDS             8913..10238
                     /gene="ftsA"
                     /locus_tag="IWM52_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAP9972444.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            10261..11502
                     /gene="ftsZ"
                     /locus_tag="IWM52_000158"
     CDS             10261..11502
                     /gene="ftsZ"
                     /locus_tag="IWM52_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAP9972445.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            11519..12196
                     /locus_tag="IWM52_000159"
     CDS             11519..12196
                     /locus_tag="IWM52_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAP9972446.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            12210..12809
                     /locus_tag="IWM52_000160"
     CDS             12210..12809
                     /locus_tag="IWM52_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAP9972447.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            12824..13111
                     /locus_tag="IWM52_000161"
     CDS             12824..13111
                     /locus_tag="IWM52_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAP9972448.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            13160..13942
                     /locus_tag="IWM52_000162"
     CDS             13160..13942
                     /locus_tag="IWM52_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAP9972449.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            13963..14670
                     /locus_tag="IWM52_000163"
     CDS             13963..14670
                     /locus_tag="IWM52_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAP9972450.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            14932..17730
                     /gene="ileS"
                     /locus_tag="IWM52_000164"
     CDS             14932..17730
                     /gene="ileS"
                     /locus_tag="IWM52_000164"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAP9972451.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(17779..19305)
                     /locus_tag="IWM52_000165"
     CDS             complement(17779..19305)
                     /locus_tag="IWM52_000165"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAP9972452.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(19320..19967)
                     /locus_tag="IWM52_000166"
     CDS             complement(19320..19967)
                     /locus_tag="IWM52_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAP9972453.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(20151..20480)
                     /locus_tag="IWM52_000167"
     CDS             complement(20151..20480)
                     /locus_tag="IWM52_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAP9972454.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            20657..21385
                     /gene="sfsA"
                     /locus_tag="IWM52_000168"
     CDS             20657..21385
                     /gene="sfsA"
                     /locus_tag="IWM52_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAP9972455.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            21401..22414
                     /locus_tag="IWM52_000169"
     CDS             21401..22414
                     /locus_tag="IWM52_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9972456.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            22414..23691
                     /locus_tag="IWM52_000170"
     CDS             22414..23691
                     /locus_tag="IWM52_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP9972457.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            23754..26456
                     /locus_tag="IWM52_000171"
     CDS             23754..26456
                     /locus_tag="IWM52_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9972458.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            26608..26925
                     /locus_tag="IWM52_000172"
     CDS             26608..26925
                     /locus_tag="IWM52_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9972459.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            26955..27275
                     /locus_tag="IWM52_000173"
     CDS             26955..27275
                     /locus_tag="IWM52_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9972460.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            27383..28843
                     /locus_tag="IWM52_000174"
     CDS             27383..28843
                     /locus_tag="IWM52_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9972461.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            28911..29132
                     /locus_tag="IWM52_000175"
     CDS             28911..29132
                     /locus_tag="IWM52_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972462.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            29163..29345
                     /locus_tag="IWM52_000176"
     CDS             29163..29345
                     /locus_tag="IWM52_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAP9972463.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            29345..29758
                     /locus_tag="IWM52_000177"
     CDS             29345..29758
                     /locus_tag="IWM52_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAP9972464.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            29881..31062
                     /locus_tag="IWM52_000178"
     CDS             29881..31062
                     /locus_tag="IWM52_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9972465.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            31451..31524
                     /locus_tag="IWM52_000179"
     tRNA            31451..31524
                     /locus_tag="IWM52_000179"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31485..31487,aa:Arg,seq:cct)
     gene            complement(31593..32732)
                     /locus_tag="IWM52_000180"
     CDS             complement(31593..32732)
                     /locus_tag="IWM52_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9972466.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(33031..33666)
                     /locus_tag="IWM52_000181"
     CDS             complement(33031..33666)
                     /locus_tag="IWM52_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972467.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINSASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(33779..34414)
                     /locus_tag="IWM52_000182"
     CDS             complement(33779..34414)
                     /locus_tag="IWM52_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HAP9972468.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(34448..34909)
                     /locus_tag="IWM52_000183"
     CDS             complement(34448..34909)
                     /locus_tag="IWM52_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HAP9972469.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(35039..35470)
                     /locus_tag="IWM52_000184"
     CDS             complement(35039..35470)
                     /locus_tag="IWM52_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP9972470.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(35488..35808)
                     /locus_tag="IWM52_000185"
     CDS             complement(35488..35808)
                     /locus_tag="IWM52_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9972471.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            36107..36883
                     /locus_tag="IWM52_000186"
     CDS             36107..36883
                     /locus_tag="IWM52_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP9972472.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            36898..37101
                     /locus_tag="IWM52_000187"
     CDS             36898..37101
                     /locus_tag="IWM52_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9972473.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            37117..37455
                     /locus_tag="IWM52_000188"
     CDS             37117..37455
                     /locus_tag="IWM52_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAP9972474.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            37442..37621
                     /locus_tag="IWM52_000189"
     CDS             37442..37621
                     /locus_tag="IWM52_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972475.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(37664..38134)
                     /locus_tag="IWM52_000190"
     CDS             complement(37664..38134)
                     /locus_tag="IWM52_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972476.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            38221..38919
                     /locus_tag="IWM52_000191"
     CDS             38221..38919
                     /locus_tag="IWM52_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP9972477.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            39097..39438
                     /locus_tag="IWM52_000192"
     CDS             39097..39438
                     /locus_tag="IWM52_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972478.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            39431..40102
                     /locus_tag="IWM52_000193"
     CDS             39431..40102
                     /locus_tag="IWM52_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP9972479.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            40108..40794
                     /locus_tag="IWM52_000194"
     CDS             40108..40794
                     /locus_tag="IWM52_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972480.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            40797..41546
                     /locus_tag="IWM52_000195"
     CDS             40797..41546
                     /locus_tag="IWM52_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9972481.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            41558..41827
                     /locus_tag="IWM52_000196"
     CDS             41558..41827
                     /locus_tag="IWM52_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972482.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            41989..42291
                     /locus_tag="IWM52_000197"
     CDS             41989..42291
                     /locus_tag="IWM52_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972483.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            42288..42449
                     /locus_tag="IWM52_000198"
     CDS             42288..42449
                     /locus_tag="IWM52_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP9972484.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            42446..42751
                     /locus_tag="IWM52_000199"
     CDS             42446..42751
                     /locus_tag="IWM52_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972485.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            42751..43107
                     /locus_tag="IWM52_000200"
     CDS             42751..43107
                     /locus_tag="IWM52_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAP9972486.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            43067..43312
                     /locus_tag="IWM52_000201"
     CDS             43067..43312
                     /locus_tag="IWM52_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972487.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            43309..43728
                     /locus_tag="IWM52_000202"
     CDS             43309..43728
                     /locus_tag="IWM52_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAP9972488.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            43725..44282
                     /locus_tag="IWM52_000203"
     CDS             43725..44282
                     /locus_tag="IWM52_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP9972489.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            44279..44575
                     /locus_tag="IWM52_000204"
     CDS             44279..44575
                     /locus_tag="IWM52_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972490.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            44652..45065
                     /locus_tag="IWM52_000205"
     CDS             44652..45065
                     /locus_tag="IWM52_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP9972491.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            complement(45523..45798)
                     /locus_tag="IWM52_000206"
     CDS             complement(45523..45798)
                     /locus_tag="IWM52_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972492.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            46252..46458
                     /locus_tag="IWM52_000207"
     CDS             46252..46458
                     /locus_tag="IWM52_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972493.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            46654..46821
                     /locus_tag="IWM52_000208"
     CDS             46654..46821
                     /locus_tag="IWM52_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP9972494.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            46847..47191
                     /locus_tag="IWM52_000209"
     CDS             46847..47191
                     /locus_tag="IWM52_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP9972495.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            47196..47477
                     /locus_tag="IWM52_000210"
     CDS             47196..47477
                     /locus_tag="IWM52_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972496.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            47580..47894
                     /locus_tag="IWM52_000211"
     CDS             47580..47894
                     /locus_tag="IWM52_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAP9972497.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            47872..49566
                     /locus_tag="IWM52_000212"
     CDS             47872..49566
                     /locus_tag="IWM52_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP9972498.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            49586..50764
                     /locus_tag="IWM52_000213"
     CDS             49586..50764
                     /locus_tag="IWM52_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP9972499.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            50727..51413
                     /locus_tag="IWM52_000214"
     CDS             50727..51413
                     /locus_tag="IWM52_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP9972500.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            51413..52573
                     /locus_tag="IWM52_000215"
     CDS             51413..52573
                     /locus_tag="IWM52_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP9972501.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            52583..53458
                     /locus_tag="IWM52_000216"
     CDS             52583..53458
                     /locus_tag="IWM52_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972502.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            53455..53766
                     /locus_tag="IWM52_000217"
     CDS             53455..53766
                     /locus_tag="IWM52_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972503.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            53756..54109
                     /locus_tag="IWM52_000218"
     CDS             53756..54109
                     /locus_tag="IWM52_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP9972504.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            54099..54500
                     /locus_tag="IWM52_000219"
     CDS             54099..54500
                     /locus_tag="IWM52_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972505.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            54493..54897
                     /locus_tag="IWM52_000220"
     CDS             54493..54897
                     /locus_tag="IWM52_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972506.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            54909..55514
                     /locus_tag="IWM52_000221"
     CDS             54909..55514
                     /locus_tag="IWM52_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP9972507.1"
                     /translation="MAKTTTVTTFDNISIKRISFNFKNATNAISTDCNGQLDGETEMQ
                     RIVKKCGSTEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDERIKSGIYSYGPDSVG
                     EDFSLAAEIVDDFEEKNKLIGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFGK
                     FYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            55534..55896
                     /locus_tag="IWM52_000222"
     CDS             55534..55896
                     /locus_tag="IWM52_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972508.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            55899..56081
                     /locus_tag="IWM52_000223"
     CDS             55899..56081
                     /locus_tag="IWM52_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972509.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            56098..59529
                     /locus_tag="IWM52_000224"
     CDS             56098..59529
                     /locus_tag="IWM52_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP9972510.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDFIVAIGGLTAAFGILLPAILAVSYLFGPMML
                     IIGGIIAVIAGVIVAIKNWGAITDWFSGLWKKFTDWLGDTWESIKDGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPEFIMEGSF
                     NPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGVGEAGPEAVLPLKRSVLQEIGDRILS
                     STSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
     gene            59580..60317
                     /locus_tag="IWM52_000225"
     CDS             59580..60317
                     /locus_tag="IWM52_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP9972511.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            60327..62618
                     /locus_tag="IWM52_000226"
     CDS             60327..62618
                     /locus_tag="IWM52_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972512.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVDGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            62642..64768
                     /locus_tag="IWM52_000227"
     CDS             62642..64768
                     /locus_tag="IWM52_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972513.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSENPNLMPYITEPWVG
                     PLLGNGHTVKDSVKRVITHTKTRTANSGDILSLGLGIPCTAEANNRYLITTLRPSTTY
                     TLSVTMSVGSDWTGETNTIGVRLRYLNEQGGIELPINALIPANVERDKMVTHTFTGIT
                     KDNVTSITNCYVEIFSLNSEYKGTVSVSYDVKLKAHYPNLLDGPYWLGKVPLGENIAD
                     PTVVFPHKTSEYMVYGRRNTENYIADQTYTISMKATKLTVQSFAVYIAAGRVKVGDMK
                     PTEGLANTWELTFTVTKQHIDSGVTNYLEIYQYPSATKGAVQIEWLKLEKGNTRTPNI
                     SEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINS
                     QLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKL
                     YREGKTVSIYFVALNGKRSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSI
                     GADGAIKWRRNSSYGSDYTFAITYTI"
     gene            64931..65377
                     /locus_tag="IWM52_000228"
     CDS             64931..65377
                     /locus_tag="IWM52_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972514.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            65379..65516
                     /locus_tag="IWM52_000229"
     CDS             65379..65516
                     /locus_tag="IWM52_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP9972515.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            65554..65847
                     /locus_tag="IWM52_000230"
     CDS             65554..65847
                     /locus_tag="IWM52_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972516.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            65844..66068
                     /locus_tag="IWM52_000231"
     CDS             65844..66068
                     /locus_tag="IWM52_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP9972517.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            66065..67090
                     /locus_tag="IWM52_000232"
     CDS             66065..67090
                     /locus_tag="IWM52_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9972518.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
BASE COUNT        23106 a        10922 c        13926 g        19298 t
ORIGIN      
        1 taattttagt tagtggagga atggggggct ttcaatgttg atgggtgaat tccaacataa
       61 tattgatgca aaaggacgtt taatcgtgcc ctcaaaactt cgtgaagaac ttggcgaaaa
      121 gtttgttctg acacgcggat tggatggctg tcttttcggc tatccgatga gcgaatggga
      181 aaatctggaa gctaaactga acgaaatgcc gttggctaaa aaagacgcac gtacttttgt
      241 gcgattcttc tattcggctg ccactgaatg cgaacttgat aaacaaggac gaatcaatat
      301 acccagcacg ttaagaaatt atgcagcttt gacaaaaggt tgcgtagtca tcggtgtatc
      361 taaccgaatt gaaatttggg atgagacacg atggcaagat ttttctgcag cagcagaaga
      421 gaactttgac gaaattgctg aaagtatgat tgatttcgga ctataagaat aggaagaggc
      481 tgactaatga ctgaaacatt tcaacactat acagtcatgt tgaaagaaac ggtagatgga
      541 ttgaacatca aaccggatgg cgtttatgta gactgtacac tcggcggtgc cggacatagt
      601 gaatatttat taagccaact aagttctaaa gggcatctgt atgcgtttga ccaagatcaa
      661 aaagcaatcg atcacgccaa gctgcgttta gcttcttatg tcgaacaagg tatggtcaca
      721 tttatcaaag cgaacttcag agaattagaa gaacgcttga gtgagcatgt agataaggta
      781 gacggcattt tgtatgatct aggggtttcc tctcctcagc tagatgaagc agaacgtgga
      841 tttagttatc atcaagatgc accgttggat atgcgcatgg atcagtcagc tcctttatcc
      901 gcttatcacg taattaatga atattcctac catgaattag taaagatttt cttccgctat
      961 ggcgaggaga aattttccaa acaaattgct cgtgaaatcg aacgagtacg aaaaacaaaa
     1021 ccaatccaaa caactggtga gcttgtcgaa atcattaaac aagtcatccc agctcccgca
     1081 agaagaaaag gcgggcatcc tgcaaaaaga atttttcaag ctgtgcggat tgcagtaaat
     1141 gacgaactcg gtgccgaaga ggcctcttta gaacaggcaa tccggttatt gaaagtcaat
     1201 ggaagaatca gcgtaatcac attccattca ttggaagaca gaattgtcaa aagtatgttt
     1261 aaagaatata gtacggtaca agatctacca ccgggacttc ctgtcatacc tgacgaattc
     1321 caaccagaac tgaaagtcat tactcgtaaa ccaatccttc cttcagagga agagctaaca
     1381 gaaaacaatc gatcacgcag tgcaaagtta cgtattgcag aaaaaataag aatgaaagag
     1441 gagtagaatg atggcagaat taaagaaaat gcaagattac gaatatgatt tacctgaatt
     1501 ggtggatcag ccggaacaac aaatgcctga ttcttcttcg aatcaagaaa tcattcttcc
     1561 acaatcaccg gcaaaacgat taaagcagat ttctgctttg gaaaaaattg ctgtggcaag
     1621 cgtggcgatt gctattgttg ctttatgtgt tttaacagtt atgctgcgca caaatatcag
     1681 cggagctgaa aaagaaatca cgtcaataca aacagaaatc aatgataaaa agcaagaaaa
     1741 aacgagttta gaacaagaaa aaaacgaact ttctcgtact gaacggatta aacaaatcgc
     1801 agaagaaaaa ggattgtcta ttaatgacga caatttaagg aaagtgaagt aaatgagtct
     1861 taaaaataaa attcggcggt ttatggaaaa gaaaaatcta aatccgatga acaatcgcaa
     1921 aaaagtaggt atcattttat ttgctacgag tattggattg ttctttttat ttgccgtccg
     1981 tttctcgtat atcgttattg gaggtcatgt tgctggtacc tcattagctg aaaaaacaaa
     2041 acagttgtac caaggaagtg aagtagtaaa agcaaaaaga ggaacgattt atgatcgtaa
     2101 cggtgtggcg ctcgcagaag atgcctcctc ttattcaatt aaagcgatac tctcaaaaac
     2161 atatacctct ggggataaaa aactgtatgt tgaagaaaag aattttgata aaatagcaga
     2221 aatcctgcat aagaatcttt ctatagacaa aaaagatgca ctgaatattt tagaggacgg
     2281 cgcaaaaaaa gagctgtatc aagttgaatt tggtagctat gggaaaaata tctcccaaga
     2341 aaccaagcaa aacatcgaag cggacatgaa aaaagaaggt gtcgctggtc tttattttgt
     2401 cgaccatcag gctcggatgt atccaaatgg cgtcttttct tctcatttta ttggatacgc
     2461 agttccagac aaagatgaga atggcttagt tggtaaacta ggtttggagt ctgcttacaa
     2521 tgatatcttg agcggtaaag atggtaagat tatttatcaa aaagataatt ttcaaaatcc
     2581 tctgccaggt acagtggcag aagaagaaaa agctgtcgac ggacaagata tctacacgac
     2641 tttagacagc agactacaaa gttatttaga aacactgatg gatcaagtaa atgaagaata
     2701 tcagccagaa gaattgacag cagtcctgat gaaagcaaaa accggagaaa tcttggcaat
     2761 gggacaacga cctactttca atcctgaaac aatggaagga ttgactggaa aagatgctat
     2821 ctggcgtaat ttcttggtac aagatagtta tgaaccagga tcaaccatga aagttttcac
     2881 tactgcagct gctatcgaag aaggagaatt caacgagaat gaaaccttcc aatctggaaa
     2941 aatccaagta gaagatgcta cgatcaatga ccatgacttt ggtgaaaaag gtgtgctgac
     3001 catgcgtcaa gctctttcat ggtcaagtaa cgtcgggatg gtcatactgg aacagcgatt
     3061 aggcggacga tggtataact atttacaaaa actgggattt ggacaaagta ctcattctgg
     3121 attagatgat gaagtaaacg gtgctttacc tacattaaat attgtcgacc gagcaatgag
     3181 tgcttatgga caagcggtag gtgtgacgaa tttccaaatg atgaaagctt tcacttctat
     3241 tgcgaataac ggtacaatga ttcaacctcg atatatcagc aaagtggtgg atccccaaac
     3301 tggagaagag cgaacaactc aaacagaagt tctagggcaa cctttttcaa aagaaacaac
     3361 agaaaaagtc cgtgaatata tgagagatgt tgtcgaaagc gaaaactacg gtagtgctta
     3421 tggtgtatat agtgtacctg gatacaatgt atcggcaaaa aacggaacag cccaaatcgc
     3481 ttctgataca gggggatatc aaacaggaga tacagcctac ctgtattcga ttgtggagat
     3541 ggttccttct gaagatccag actatgtctt gtatctaaca atgaaacacc ctaagacata
     3601 tgatcgaatg gcattggcta aaatcgcaaa tccattgatg aaacgggcaa tggattttaa
     3661 agaaactgaa gaagattcag atacagaaac aaaaacagaa aaagtatccg tagccgatta
     3721 tcgtaatctt gaagcggacg ttgcagcagc tgatgctcaa aaaagtggac tgcaacctgt
     3781 agtgatcggt aatggtaaaa aagtacaaaa acaatctact gccaacggtg atcagttgat
     3841 ctctggagaa aaactgatcc tatatactgg tggtgataag ccgatgcctg acgtaacagg
     3901 atggtcaaaa gcagatatca tgaagctggg gaaaatcctc ggtattgaag ttagttttga
     3961 tggagatggt tattgtgtca aacaagaact tgctccatat gaaaaaatca ctgaagacaa
     4021 acttagtttt actttagaag aataagaaga gaaataggag agaaaagagc atggaatgga
     4081 cacaagcatt gattcccatt gtcagcagtt gtgcaatgac aattgccgca atgccgctat
     4141 ttattggtta ttttcaaatg aaaaagcaag gtcaggcgat tcgtgaggag ggaccaaagt
     4201 ggcacaatag caaagccgga actcctacaa tgggcggctt agtgtttctg atcggctcta
     4261 ttttgacagg aatttgggtt ggagcttggc aaaaacagct aactcctact cttttcatct
     4321 tgctttttgt attagcgtta tacggagtaa tcggctttct tgatgatttt ataaaaatat
     4381 tcaagaaaag aaacatggga ctgaactcga agcagaaatt gattggacag attctagggg
     4441 gaatcatctt ttatcttgtt taccgttctg aaggttatcc aggtgtattg aatttcttcg
     4501 gactagatct acctttagga atcatttatg gtattttcgc tctcttctgg cttgttggat
     4561 tttcaaatgc agtaaatctg acagatggca tcgatggatt agttgctggc ttaggaagta
     4621 tctcttttgc agcttacggt ctaattgctt ggcatcagca tcagtatgat gtattaatca
     4681 tttgcttaag tgttttagga ggtttactgg gtttcttcgt ctacaacaga aaacctgcca
     4741 agatttttat gggagatgtc ggttcattag cgttgggcgg acttttagcg gcaatttcta
     4801 taatgctaaa ccaagaatgg acattgcttt tgattggttt gatctatgtt atggaaacag
     4861 caagtgtgat gcttcaagtt acttccttta agcttacagg aaaacgaatt tttaaaatgt
     4921 ctccaattca tcatcacttt gaaatgtgcg gatggtcgga gtggaagatt gatacagttt
     4981 tttggttgat cagcattgtg acttcgttga tcacattatg gatcgtttgg taggagatga
     5041 cgggaaatga aaaaaatatc aacatatgaa aataagaaag tgcttgtctt aggactcgca
     5101 aaaagcggag taagtgcggc caaattatta catgaattgg gcgcacttgt caccgttaat
     5161 gatggaaaac cttttgacga aaatccggaa gcacaagagc tgctttcttt agggataaaa
     5221 gtaataacag gtagtcatcc aatcgaattg ttggatgaag aattttcatt gatggtcaaa
     5281 aatccaggga ttccctacag tcatccttta gtagcaaaag cccaagaaat gggtattccg
     5341 gtaattacgg aagttgagct agcttacgaa gtagcggaat gcccaattat tgggattaca
     5401 ggaacgaacg gaaaaacaac gacaacaacg atgacaggac tactactgaa tgcaggagca
     5461 gatcaaggaa ttgcacgttt agcgggtaat attgggtatc cagcaagtgg tgtagcgcaa
     5521 gaagcaaaat cagaagataa aattgtgatg gaattgtcta gcttccaact catgggaatc
     5581 acagatttcc gccctcatat agcggtgatt accaatatct atgaagcaca tattgattat
     5641 catggtacaa gaaaagaata tgtcaaagct aaatggaacc ttcaaaaaaa catgacagaa
     5701 aaagattatc tgatccttaa ctggaatcag tcagaactac aagaattagc gcaaagaaca
     5761 aaagcacgcg tactgccttt ctcgacaaaa gaagtgttgg aagacggtgt gtatgctgac
     5821 gattactcaa tatactataa aaaagaaaaa ataatggaaa tatctgaact tggcgtacca
     5881 ggtaagcata atgtagaaaa tgcactagct gctatttctg tagcaaaatt gtatggtata
     5941 tcaaatgaag ctatacgaga aacgttgcat tttttccatg gcgtaccaca tcgtacgcaa
     6001 tatgtaggag aaatccaagg cagaaaattt tataatgatt caaaagcaac caatatttta
     6061 gcgactaaaa tggctctaag tggatttgaa acgtctaagg tcgtgttatt ggctggcgga
     6121 ttagacagag gaaatacatt tgatgaatta attccttcat taaaaggaat caaagcaatg
     6181 gtcgtatttg gccaaacaaa agaaaagttg atggatgctg gaaaaaaagc aggcatcgag
     6241 acaatagtaa cagccgattc tgtagaacaa gcagttcctt tagcattaga aaacagtaca
     6301 gatggtgatg tagttttatt atcaccagcg aatgccagct gggatcaata cccaaatttt
     6361 gaaactagag gaaacagatt catggaagct gtgaaccgat taaaatagaa aaagtagaag
     6421 cgagcgataa aaaatgaaaa ttttggttac aggcggagga acagggggac atatttatcc
     6481 agctcttgcc tttgtcaatt atgtaaagac tcaagaaccg aatgctgaat tcatgtatgt
     6541 gggagcaaaa cgaggtctag aaaacaaaat cgtccctgat acaggaatgc ctttccatac
     6601 cttggagatt caaggattta aacgaaaaat ttccatgcat aacatcaaga cggtccgatt
     6661 gtttcttaag agtatccgtc aagcaaaaaa aatcctcagt gattttcaac cagatatcgt
     6721 tatcggtact ggaggatatg tttccggagc ggtagtgtac gctgcagcca aaatgggtat
     6781 tccaacagtt atccatgaac aaaacagtgt accaggtgtt accaataaat ttttaagccg
     6841 atatgtggat cggatcgctc tttcatttaa agatgcggct tccttttttc ctgaaaacaa
     6901 agcagttctt gtaggtaatc ctcgagcaca agaagtagca gatactaaaa aatcagatat
     6961 tttgaaaaca tttgatttgg atccagaaaa gaaaacagtc ttgatttttg gcggtagtca
     7021 aggagcactt aaaatcaatc aagcagtatc agttttttta agtgaatata ttccttcttc
     7081 ttaccaagtt ttgtatgctt caggagatag atattatgaa gagatcctaa ataaaataaa
     7141 tcctgaaata caaaatatta gtgtccagcc ttatatcaaa aatatggcag aggtcatggc
     7201 ttccagtgat ctgctggtgg ggcgtgctgg tgcaacttcg atcgcagaat tcacagctct
     7261 ggggttaccg gcagtgttgg ttcccagccc atatgttacg aatgatcatc agactaaaaa
     7321 tgcaatgagt cttgttcacg caggagcagc aaaaatgatt gcagataatg aactaacagg
     7381 tgaatcacta agtcagacag tcaacgaaat catgggtgat gaagaattgc aaaagcaaat
     7441 gtgccgagct tctaaagaac aaggaatccc tgatgcttca aaacgtcttt atgacttagt
     7501 aaaacaaata atcaaaaaat aaaggcggtg gtcttagtga gtaagaaaaa agaaccaaaa
     7561 aaattgtctc atccgtcttc tgatgaaaac aatttgactc cttggcaaaa ggctaaccga
     7621 aaatatctgg cagaacaaga gatgaaaaac caagagccaa aaaaagaaga cataacatta
     7681 gatacacccc tttcagattc tgaagaagaa aaaacggttc aagaggaaca gcaagaagaa
     7741 agccagtccg agaaaaaaat cgaatttttt gaagagatca agcaaaagaa aaacggtgga
     7801 ccatataacg gttctttttt gaaccggctg cccaatttaa aaagtcaacg gcacaaagta
     7861 ctatttcgtc ggttagcttt gatcatcact gtattgggta taccgctgat ttttttgatc
     7921 tattatgttt ctccttatag caaattacaa gcagttgcag tatcaggcaa taagacagtc
     7981 aacagccaag aaatcatctc tgatacaaaa ttgtcattag gagaaaatgt ttggggacag
     8041 tattttcatc gatctactta tatcgatcgc cttaaaaaag cacagccaag aatcgaaaca
     8101 gcaaatatac atttcaaagg gatgaatgaa ttcgatttgg atgtaacaga atataaagag
     8161 attgctttga tcgcaaaaaa caatcaatac tacccagtga ttgaaaatgg aacagtatta
     8221 gatgaaaaag tagcaaatcc tacaaaaaat ctccctatct tggaagagtt caaagactct
     8281 gcaaaaatca aagaattaac aaagcaatac aatcagttat catcagaatt acaaaaagca
     8341 atttctgaaa tcaagtacac gccaagagca agcaataaaa atctgatcca attgaatatg
     8401 aatgacggca atcaagttat tgtaaatatc aataatcttg cgaaccaaat gaaatattat
     8461 tcgcaagtag ccaaagatat ggatgaaaaa ggtgtgatcg acatggaagt tggtatcttc
     8521 tcgcatcctt acggtagcac tcaagaaacg gaagaatcgg gccaatcgga agaaacaact
     8581 gaaagtgaag aaaatgcgga tcaaacaaac gaatcaagtg aagaggcaga aacgcaaaaa
     8641 gcacaaacac ctggatcgtc tagcacaaac ggatcatctg attcaaatga ttcaagtgaa
     8701 acaagtcaaa tagaagataa tccaagtaca actccgtcaa gtttaaatga aaattgataa
     8761 aacaacttta aaaagtaaga atgtactttc taaaaaccag taatttatgg tagaattgga
     8821 agcagtgaat atatctatta ataagtatat tttaaaacgg acgctagttg ttcgcttgta
     8881 tatggaatag taagtaggag ggaatcccat tcatggcaaa aacaggaatg tatgtaggcc
     8941 ttgatattgg aacgacatct gttaaagttg tcgtggctga atatatcgac agccaaatga
     9001 atattattgg agtaggaaac gcaaaatcag aagggattaa ccgaggcatt atcgttgata
     9061 tcgataaaac ggtccaagcg atacaacgag cagtacgaca agcagaagaa aaagcaggta
     9121 tccagatcaa aggcgtaagt gtcggtttgc cagcaaacat gcttgaagta gaaaattgcc
     9181 agggaatgat tgcagttaac ggagattcaa aggaaatcac cgatgaagat gtcagaaatg
     9241 tggcttcagc tgcccttgtt cgttcaatac ctcctgaacg tcaaatcgtg tcaatattgc
     9301 cacaagattt tactgtcgat gggttcgagg gaatcaaaga tcctcgtgga atgatcggcg
     9361 taagattaga aatgtacggt ctgttgttta caggacctaa aacgatcatc cataacatac
     9421 gtaaatgcgt ggaaaatgcc ggattaatcg tcaacgaaat ggtgattact cctttagcgc
     9481 tgactgaatc aattctttct gacggagaaa aagattttgg tacgatcgtc attgatatgg
     9541 gtggcggcca aacaacaacg gctgttatgc atgataagca attgaagttt accaatcttg
     9601 accaagaagg tggcgaattt gtcactaaag atatctctat tgtattaaat acgtcattta
     9661 acaatgcaga agcgttgaaa ataaactacg gggatgcata cccagaacgg acatcaccag
     9721 atgaagaatt tccagtagat gtgattggtc agtcagaacc agtgaaagtc gatgaacgct
     9781 acttgtcaga aattatttca gctcgaatgg aacagatttt caacaaagca aaagatgcat
     9841 tggaccaaat cgaagcatta gagttgccag gtggcgtcgt attgacaggc ggagctgcta
     9901 gtcttcctgg tgttgtggac ttggcacaag aaatcttcgg cgtaaatgta aaattgtatg
     9961 ttccaaatca catggggctg agaaacccgg tatttacaaa tgtaataagt atcgtggatt
    10021 attcagcaaa cttgagtgaa gtgtatcaat tggcaaaaat tgctgttacg ggtgaaacct
    10081 cagcagctcg tcaaatggta gttgaacaag aaacaacaaa cacatatgag agctatgaag
    10141 cacctgaaga aacaatctac gatgagccgg aaccaaaaga gtccggagaa aatgtaaaaa
    10201 ataaaatcaa gggtttcttt acaaatattt ttgattaatt catcctggga ggaataagat
    10261 atggaattct caattgataa taatatcaat gacggcgcag taatcaaagt catcggagtc
    10321 ggtggtggcg gtggaaatgc tgtaaaccga atgattgaag aaaacgttaa aggtgttgaa
    10381 ttcattacag ccaacacaga cgtgcaagca ttaaaaaatt caaaagctga aacagttatc
    10441 caattaggcc ccaaatatac tcgtggttta ggtgctggtt ctcagccaga agttggacaa
    10501 aaagcggcag aagaaagtga acaatcctta cgcgaagctt tagacggtgc agatatgatt
    10561 ttcatcactg caggaatggg tggaggaacc ggtacaggtg ctgcaccaat cgttgcaggt
    10621 atcgcgaaag aattgggcgc attgactgtt ggtgtcgtga cacgtccatt cacttttgaa
    10681 ggaccgaaac gtggccgttt tgctgcagaa ggtatcgctc gtttgaaaga aaatgtggat
    10741 acattattaa ttatctcaaa taaccgttta ttagaagttg tagataaaaa gacaccgatg
    10801 cttgaagctt tccgtgaagc agataatgtt ttacgacaag gtgtacaagg tatctctgat
    10861 ttgatcacag cgccaggtta cgttaacctt gactttgctg acgtgaaaac agtgatggaa
    10921 aaccaaggta cagcattgat ggggatcggt gtggctagtg gagaagatcg cgtagtagaa
    10981 gcaacgaaaa aagcaatctc ctctccattg cttgaaacat ctattgatgg agctgaacaa
    11041 gtacttctta acatcacagg cggtttggat atgacattgt tcgaagcaca agatgcttca
    11101 gatatcgtag caaatgcagc aactggcgat gtaaatatca ttctaggtac atctatcaat
    11161 gaagaaatgg gcgatgaaat ccgtgtgacg gtcatcgcaa caggtatcga tgaatcaaaa
    11221 aaggaacgca agtctagccg accagctaga caagcacaaa tgcaatcacc tgctcaaaag
    11281 actgtattag atatggatca agctaaacca acttcttcag aagaagaaaa cagttttggc
    11341 gattgggata tcagacgtga acaaaatgtt cgtcctagag ttgatgattc caattttgac
    11401 aatatcgaaa aaaaagaatt tgatacattc aaccgcgaag aaacaaaatc tcatggcgat
    11461 gatgaattaa atacgccacc gtttttccgt cgtaaaaaat aaaggagagg aagagcacat
    11521 gattgctgac aacttgcaga aaatcaagca agaaattcag gattcgtgtg ctcttgttca
    11581 tcgtgatccg aaagaagtta cattagtagc tgtaacaaaa tctgttgaat ccgctcaagc
    11641 tgaagaattg gttagagctg gcgttatgaa ttgtgcagaa aaccgagtag ataaattatt
    11701 agaaaagaaa caagatttat ttgattttcc tgaaataaag tggcatttaa ttggtaattt
    11761 acagcgcaga aaggtaaaat tgattgtaaa tgaaattgat tactttcatg cgttggacag
    11821 tttaagatta gcagaagaaa tccagaagcg gttagagaaa gaattatcct gttttattga
    11881 agtaaatgtt tctggtgagg aatcaaaaca tggaatttca ccagatgagc ttttaccgtt
    11941 tgtggaatct ttagctgcct gcacaaaaat caaggttgtt gggttgatga ctatggcgcc
    12001 taaagaagct tctgatgagc aaatccataa agtgtttggt acgctgaaag aattgcgtga
    12061 tcttgttcaa gcaaaaggtt atgcccatgc accatgtagt gaattaagta tggggatgag
    12121 tcaagacttt caaattgcga ttgaagaagg tgcaacattt atccgtgtag gaacagcgct
    12181 attcaaagac gaataaagga ggaacaaaaa tgccgctttt aaacagagga gcaattgcta
    12241 attttttcgg attagctgat gaggaagagt acgaatacca agattatcct gaaacaacaa
    12301 aaaaggctgt acagccaact aaaaaaaata caacacagaa tcaatcaact cattctgtat
    12361 ctgctgcgaa tactcatgaa aaacaaaaaa cggcgacttc acgtcctgta acgcatgaaa
    12421 gtattcagcc ggctgaaaag aaaaaagtaa ttgaactaca tcaatcacct acaaaaactg
    12481 tagcgaaaga aagaacagct tcaagaacct ctacacaacc taaagcaaat cttgctggaa
    12541 aaatcactgt aatggaacca agagcttatt ctgaagctat ggcgattgct aaacgaatca
    12601 ttgctggaga agcaacctta gtgaattttc atttagtaga agaaaaacaa gcgagaagaa
    12661 tcgttgattt tctaacagga acagtttatg cactagatgg agatatcaaa cgtgttggag
    12721 atgagatttt tctttgtaca ccgactggga tggagattga cagttcaacc gctcaatctt
    12781 ttgcagatgc gaatttgttt gatctgtaag aggaggaact acgatttata ctattgcact
    12841 attacttcat ttacttcaac gggcagccta tttctatagc attatcttga tggtatatgc
    12901 attgctctct tggtttccag gcgggtatca atcagggcta ggacgattat tagctaagat
    12961 ctgcgaaccg tatatcagtt tgttcgatcg tctgcctttg aggattggac ccttggattt
    13021 cacaatttta ggggcaatcc tgatcttgaa cttagccgta caaggtttag cacagatcgt
    13081 tatatggata actgtccatt tagtttattg attgaagcaa caaaatcttt attctcagaa
    13141 tagataggag gggaaaattt tggatgcaaa tgtataccag cactttcggg cagatgaacg
    13201 tacatttatc gattcggtag gcgattggat tgaacaggtt caaggacaat atgcacccta
    13261 tctgaccgaa tttcttgacc caagacaatc ctatatttta gaaacactga ttcgacaaag
    13321 cagtgatttg cgtttcatgt tttatggcgg atatgaacag gcagaaagaa aaagatgttt
    13381 gattttcccc gattattacg aacctgtaga ggaagatttt gaaacagcat tgtatgaaat
    13441 ccattatcct agcaaatttg ccagcctaag tcatgggaaa atccttggaa ctttagtggg
    13501 aacggggatc aaacgctctt actttggcga tatcatctcg gacggtgatc gctggcaagt
    13561 atttattgca aatgaggtgg atcattttgt tgctgcacaa gtaacaaaga ttggaaaggt
    13621 tgctgtacgc ttggaaccaa tcaaatacac agaaatgatt atgccgaaag atagctggac
    13681 acaagaaaga ggaacagtta gttccatgcg actagacagt gtgatctctg agatttataa
    13741 tatttccaga cagcggtcca agcagctgat cgaagctggt aaagtaaaag tgaactggac
    13801 agaaaataca agacctgatt ttgtacttga actattagat attatttcag tcagaggtta
    13861 tggcagagtc cagatccaag aacttgaggg gaagacgaaa aaagaaaagt acagggtact
    13921 gttcggtgta ttacgtaaat aaaataaaaa ggtggtttac tcatggcttt aactccatta
    13981 gatatccaaa ataagacatt cccgacaaaa atgcggggat ataatcaaga cgaagtagat
    14041 gatttcttag atttagttgt tcgtgactat gaggaattga ctcaaagaaa tcgagaatta
    14101 gaaaaagcag tcaaacattc tgaagaaaaa cttgagtact tcaatgaatt aaaagatgct
    14161 ttgaaccaat caatcatcgt agctcaagat acggctgata aagtaaaaac aagtgcaagc
    14221 aaggaatccg aagtgatcgt tacttctgca caaaacaaag cagatgagtt agttgcgaac
    14281 gctgaaaaac gtgcacatca attaacaact gatgcagaag aaaaagcaag aaaaatcttg
    14341 acagatgcaa cagaaaaagc aagacagctt gcgacagaaa ctgaagatct taagaaaaaa
    14401 acacgagtat tccatcaacg tatcagcttg atgcttgagt ctcaattaga acaagtgaaa
    14461 agtccggaat gggatgaaat tttacaacca ttctccagct acgtgactga cagccatgaa
    14521 gtgatcaaag aagttttagc aaaacagctt gacaatgaaa atgatactga tgtaaactca
    14581 gacacaggag tcatagaagc acctatgacg gcatttgata cacaggctat tgatatctcg
    14641 atcgtgcctg gtgaaaatga aaacgagtag aacagaaaag cactttgtta ttcttaaagc
    14701 gagccgatga gagtggaagt tcggtaagac actgcatggt gaaagatcct ctacttgaga
    14761 cctgcttttc cgaaattaga gtaagaagca gcggctgacc tcgttattgg tcacagagag
    14821 tttgcactag atagatgcaa ctaaatttgg gtggtaccac ggcatgttcg tcccttgcgg
    14881 ctgaacatgc ttttttgtgt ccggaaaaca aataaaggag tgtatggttg aatgaaaatg
    14941 aaagaaacct tgcaattagg aaaaacaaaa ttccctatgc gtggtaattt gccaaaccga
    15001 gaaggcgagt ggcaaaaaga atgggaagaa aatcgaatct accaaaaaag acaagagttg
    15061 aacgaaggaa aacctagctt tgttcttcat gatggacctc catatgccaa tggtaatatc
    15121 catttaggac attcattgaa taaaatcagt aaagacatta tcattcgttc aaaatcaatg
    15181 tctggctatc gtgcaccata tgttccagga tgggatacac atggattgcc gattgaacaa
    15241 gtattggcta ataaaggcgt gaaacgtaaa gaaatgtcca tggcagaata cttggaaaaa
    15301 tgtcgtgaat atgcactttc ccaagtagat aagcaacgag cagatttcaa acgcctagga
    15361 gttgcaggag attgggaaca tccttatgtt acattagatc cttcttatga agctgcacaa
    15421 gtacgcgtat ttggtaaaat ggctgaaaaa ggctatattt acaaaggatt gaaaccaatt
    15481 tactggtctc cgtcaagtga gtcttcttta gcggaagctg aaattgaata taaagatgta
    15541 aaatctccat ctatttttgt agctttcaat gtcaaagacg gcaaagatat tcttgatgaa
    15601 gatacagctt ttgtcatttg gacgacaaca ccatggacat tgccagcaaa ccaaggtatt
    15661 gctgtaaatc caaaatttac gtatgtattg gtggaagccg atggccgtaa gtttgtcgta
    15721 gcgaaagatt tgctagaaac agtccaatca gaaatcggtt gggaaaatgt aacagttcta
    15781 aaagaatttg ctggacaaga aatggaatat atgactgcaa gtcatccatt ctatgaacgt
    15841 gaatcacttg tgattttagg cgatcatgtt actttagacg ctggtactgg attagtccat
    15901 actgctccag gacatgggga agatgactat atcgctggta gcaaatataa actatctgta
    15961 gtgtcacctg tagactcaaa aggattgttt acagaagaag cacctggatt tgaaggtatc
    16021 ttttatgaca aagcaaatcc tatgatcact gatctattaa aagaaaaagg agctttgctg
    16081 aagctagatt tctttactca tagttatcct cacgattggc ggacgaaaaa gcctgtgatc
    16141 tatcgggcaa ctccacaatg gtttgcatct atcgataaat tccgtcagaa tattttggat
    16201 gaagtagaaa aagtagagtg ggttatccca tggggtaaaa cacgtttgta taatatgatc
    16261 cgtgaccgcg gcgattgggt aatttcccgt cagcgtgcat ggggagtacc acttccgatt
    16321 ttctatgctg aaaatggaga agcaatcatc acaccagaaa caatcgaaca tgtggcaaaa
    16381 ttatttgctg aacatggatc aattatctgg tttgaaaaag aagcgaaaga acttcttcct
    16441 gaaggattta cccacccagg ttctcctaat ggcgaattta caaaagaaaa agatatcatg
    16501 gatgtctggt ttgattctgg ttcttctcat gaagctgttt tacgtcagcg accagaatta
    16561 tcattcccag cagatatgta tttagaagga tcagaccaat atcgtggctg gttcaactca
    16621 agtatcacga ctagtgtggc tatcaatggt gtagcaccat acaaatcagt tatttctcaa
    16681 ggatttgctt tagatggtga aggccgtaag atgagtaaat ctttaggaaa catcattgca
    16741 ccagaaaaag tgatcaaaca atttggtgca gatatccttc gcttatgggt atctagtgta
    16801 gattatgaag cagatgttcg tgtttctatg gatatcttaa gtcaagtggc agaagtatat
    16861 cgtaaaatca gaaatacgat gcgtttctta ttagcgaata cagaagattt cgatcctaaa
    16921 aaagatactg tttcgtacaa tgatttacgt tctgtagaca aatatatgac cgttcgattg
    16981 aaccaagtaa tcaaagaaat ccgagaagaa gggtacgaca aatataactt catgcatatt
    17041 taccgtacag ttatgaactt cttgacggtt gatctatcgt cattctacct ggattttgct
    17101 aaagatgtag tttatattga agcagaagat gattatcaac gtcgttgcat gcaaactgtt
    17161 ttctatcaaa cagctgtagc tttgacaaaa ttattgactc ctatcattcc gcatacagca
    17221 gaagaaatct ggtcattcct gaaagaagaa gaggagtacg ttcagttaag cgagtttcct
    17281 ggttatgaag agtttgcaaa ccaagaagaa ttgatggata cctggactgc tttcatggac
    17341 ttccgtgata atgtcttaaa agcattggaa gaagcaagaa acagcaaatt gattggtaaa
    17401 tctttggaag caaaactaac tgtttatcca aaacaacaag ttcgtgaaat gttaaaagct
    17461 ttggatgcag atattgcaca attattgatc gtttcaccag actactttga agttatggca
    17521 gcagatgaac aagtgccaga taacgctatg gtatttgatg atgttgctat tacagtggaa
    17581 aaagcagacg gggaaacttg cgatcgctgt cgccaagttc gtaaagatgt aggagtagat
    17641 gaaaaacttc ctcatctatg cggtcgttgt gcaaaaatcg ttgaagactt ctatcctgaa
    17701 gctgtagctg aaggattcga agaaaagtaa aagggaaaag ggggtgtgac atgttctttg
    17761 tcacaccccc ttttgttatc aatcgtttaa ttttccacgt tcacgatacc agttatctgg
    17821 ttgccagatc cattcgaatc cgtctttttc aagtagttcg aaagctgctt taggtcccat
    17881 tgatccagct ttgtagttgg gaaaatccgg ttgtgtttgg tcccaagctt ttcgaatgac
    17941 atccacgata tgccaagatt gagcgacttc ttcccaatga gagaagttcg ttccatcacc
    18001 attcaacgca tctaatagta atttttcgta agcttctggg ctattttcaa ccatttctgc
    18061 actatttcta aattccaatt tgattggttc cgtttcaaat ccttgcccta cttctttccc
    18121 attcaaggat aaagaaaatc cttcagttgg ctgaatatag atcgttagaa cattaggtgg
    18181 aagtgtcgta tcgtcacatg gttcatcgac agatgttttg aatacattaa caggaacttg
    18241 tttgaatacg atgttgatcc gagtcccttt ttcagtcaaa cgtttgcctg tacgtacata
    18301 gaaaggaaca cctgaccaac ggaagttatc gatagtgaat ttgcctgcga cgaatgtttc
    18361 agtctctgat tgagcatcca ctttgtcttc ttcgcgatag cccttgaatt gtacaccatc
    18421 caattcacct tgagcatatt gcccgcgaac gaagttttct aacacttctt cttcgttata
    18481 aatgcggata gctttcaatg ctttgatctt ttctgtacgg atttcttttt cggaaaatgc
    18541 tactggcggt tccattgcaa gtaatgctac aacctgtaaa atatgatttt gcaccatgtc
    18601 tttaagtgcg ccactatgat cgtagtagcc cccgcgatct tctacaccga ggttttcgcc
    18661 aaaagtaatt tggatattgt cgatatagcg attgttccat tgagcctcaa aaatattatt
    18721 ggcaaaacgg atagctgaga tgttttggat catttctttt cctaaataat gatcaatcct
    18781 gaaaatatct tgttctggga atacttctct gatttgctgg ttcaattcaa aagcagactg
    18841 gtaatcagaa ccgaacggtt tttctatgat caaacgattg aagccatttt ttgtcataat
    18901 attttgtgat ttcaaatgag aaacgatcgt tccaaaaaat tgaggtgcca tagccaaata
    18961 atagacacgg ttaccgttta attgatagtg tccatctaat cggtcagaca attcttttaa
    19021 tgtctgataa tgcgttgaat catttacatc atgggattga tagtaaaaat ggcttgcaaa
    19081 ggtagttgct tcttcatctg aaggattcaa tgcacggatt gtttctttga caacttcacg
    19141 gtagtgctca tcgctccagg gacggcgagc ggttccgatt acagcaaaat gctcgcctaa
    19201 atttcctttt ctgtaaaggc ggaacaaaga cgggtacaat ttacgctggg ctaggtcacc
    19261 tgtaccgccg aatatcgtaa acaatacatt tttttcatta gacatgtgga tacactcctt
    19321 tatattttat cgacaaaaat cgtgctggcc gccttaaagc tgacggggat tttctttcta
    19381 tcattttgga tagtgatcgg tccttcataa gctgcgatgt caacgatctc atattcttcg
    19441 ttgatattca ggtcaatagt gactaagtaa tccaacaatt ctttttcatc aagcactcgt
    19501 gcgatacgca cttttgtacc gacaggatag gactccaatg tttgtcggcg ttcttcatgg
    19561 acaacttcct cttttttagg aatcgatcct ccatgaggac aatgttcagg atgattcaaa
    19621 tagttttcta aatgatctgc aagatgttcc gaagtcacat gttccaatac ttcagcatca
    19681 tcatggactt cattccagct atagtctaaa tgttcaacta aaaaaacttc ccataagcga
    19741 tgtttgcgaa tcagactgct agctttttgc agtccgcttg cagtcaattg tacaccttga
    19801 taaggggaat gttctacaag ctcttctttc acaagtttag aaatcatttc actgacagat
    19861 gcagctgaga catctagact agatacgatt tgtttattgt tgactttcgt tgtatctcca
    19921 cctagttcta aaatgatttt caaataatct tctcgatttg gtgtcatatt cgatcatcct
    19981 ttcatggtaa aaaaccatca ttagtaagtg taacaaaaaa aacgttaatt gactattttt
    20041 ctaaataaaa aacaaaggat ttttagacgt ttttacaaca aaaaacgaaa gatgatagag
    20101 aagactcatt ctttcgttta taaaggatat atatatacat tgggagggat ctatttttca
    20161 gataacaact tatatgaagt gtctgcagat tcgtcataaa gagtttgttc gggataagaa
    20221 aagataagat aaaaaccata ctgatcacgt acgactaaac gatattcatc tatttctacc
    20281 acagatgtgc caagagactg accgttgatc aaaaacgtgt aatcagatgt aatctgtaat
    20341 tgttgttttt gctggctatc actaaagaac catgtacctt gatactttga gagtccttct
    20401 gacttttccg cttgtttttt ctttttcaag taaataaaac ttgagagaac ggtaatgagt
    20461 gaaccaagaa aatagatcat tttttttatc accaaatcac tcctttatga gaaaataatc
    20521 gcatagacat attctttctt ctttatttca ttgtaccgaa taaaagctaa aatttctcta
    20581 agactttcga ctggtttttt tactgttttt ctacaaatta tgataggata catagtagga
    20641 agaaaggtga cataatttga agtatcagca agtggaaata gcttttttta ttgaccgccc
    20701 gaatcggttt atagcacatt gtatgaaaaa agacggagaa ataattattg cgcatgtcaa
    20761 aaatacaggt agaggaaaag aggtcctgat accaggcgct gaagtggcta ttgtatttgc
    20821 tcctggacct aaacggaaaa ccgcctatga tctgattgct gtaaaaaaga agggtgactg
    20881 gtttaatatc gatagtcaat tgcctaatcg gctggcaatt gatggtattt tagacggcac
    20941 gatcctttta ccaggattaa ctggtccatt tacggtatac aagcgtgaag taacttatct
    21001 tcactccaaa tttgatattt atttagagac ggtggtcgga caaaaagcat ttgttgaagt
    21061 caaaggtatg acacttgaaa ataaagcgat tggtgctttt cctgatgcgc ccacactcag
    21121 aggattgaaa catataggag aattaacgta tgccgcacaa gatggttatg ccgcttatgt
    21181 cttgtttatt gctcagttcg aacatcttca tctggcaacg atccatgaag aaatgcaacc
    21241 agcactagct gatatggttc gacatgcgca gcaaagcgga gttcaaatcc tcgcctataa
    21301 ttgtcaagta tcggcagatg aggtaactgt caagcaggcc attccatttg atgtagatat
    21361 cccttttgaa gatcctatac aatagaaaga aggtaaaata atgatcaact taggtattat
    21421 cggaacaaat tggattactc atcaatttgt ccaagcagct ttaagcacaa aaaaatatga
    21481 attgacagcg gtatattcta gacatttgga aaaagcacag gaatttggtt ctcattatga
    21541 aggggatatc gaattcgcaa cagatttgaa gacttttttc aatattactc atatggacac
    21601 catttatatt gcttctccga atagtcttca ctttagtcaa gctaagcaag caattttggc
    21661 aggaaaaaat gtgatagttg aaaagccagc tttttcgacg ccaaaagaaa tggatgaaat
    21721 catccatttg gcaaatgaac acaatgtgta tttttttgaa gcagcaagaa atatccatga
    21781 atcaggtttt aaaaagattg ccgattactt accattaaaa gacgagataa tcggtgcaaa
    21841 tttcagctat atgaaatatt cttcgcgcta tgaccaagta ttagatggag aagaaccgaa
    21901 tatcttctcg actcatttct caggtggtgc attgatggat ttaggtgttt accttgtata
    21961 tgcagctgtt gcttggtttg gaatgccaaa agaagtccat tatttcccaa gaaagatttc
    22021 tactggtgta gatggtcttg gatgggggat cttacgttat gagaattttg atgtatcgat
    22081 ccagccaggt aaaatcggtg attcttattt accgtcagag atttattttg actcaggaac
    22141 gctagtgatg aatggagtca atgcaattga acaagcacaa tttcatgatc gcgcgcataa
    22201 agaaatcatt gatttggcgg taacagctgc agaaaatcca atggcagatg aagcagagga
    22261 ttttgccaat gtcatggctc atccgaaaga tccagcttgg ggattgttgt atgaagagtg
    22321 gatcgagcta gctagaaatg tgaaccaagt gatttatgat ttaagaaaaa atggcggcat
    22381 caaattcgat gcagataatg aggaagattt ttaatgaacg aatttgtaca agtatttccc
    22441 gaaaattggc agaaaaaata tgaggataaa gggtttatct caccttctct tattcaacga
    22501 gcagtttttc agccactaag taataaacaa agtatagtag cggtttctcc tacaggttca
    22561 ggtaaaacac tggcttatct ttggcctcta ctactaaacg tggaaccagg tgaagcaagt
    22621 gcgttggtca tttttgcttc ttctcaagaa ctagctattc aagtagcaga tgttgcacgt
    22681 gaatggggaa aagacaaaga gttgaaagtc caatctttgg tgggcggagc caacgtcaaa
    22741 cgtcagattg agggtttgaa ggaaaaacca gaaatcttga ttggtacacc aggaagaata
    22801 ttggaattga tgaaagcaaa aaaaataaaa gcacatcaag tgaaaacaat ggtatttgat
    22861 gaagcagacc agctatttga cgctgggaac agccaaatca ttgatcaaat tcttcatcaa
    22921 gctccaacag agtatcagct agcttttttt tcagcgacag cagatcgttc ccttgaaagc
    22981 attgaaaaaa tcactgggaa aacgtttgag acaattgacg tgacagcaga ggacgattct
    23041 cgaaaaggac tgagacatta ctttttacgt gtaccgatac gaaagaaaga tgactacttg
    23101 agacgtctca cgcatataga agattttcat ggtctgattt tctttaatca actgactgaa
    23161 ttaggcgtaa tggaagaaaa attgctgtat agaggtgtac cggtaggaag tctggcctct
    23221 gatcagaaca agctactccg aaaagcagcg ctagaacaat tcaaaaagaa aatggtcaga
    23281 gcacttttga ccacagatgt ggcggctaga ggtttagata ttacaggtct tccttatgta
    23341 gtgaatgcag aagttccgtt aagtgaagaa gcttatttgc atcgttcggg aagaactggt
    23401 cgaatgggta atgaaggaat agtaattaca ctcgtacaag ataataatct aagagattta
    23461 aaacggcttt tacgtccatc aaatataaca cttgaagaga tttttttata cggaggtaag
    23521 ctgcaaactg agcagccaac caaagaagat gcagctcctg cagtaaataa aaagtatgcg
    23581 aaaacttttt ctggaaagga gcagaaccca gaatataaca aagaaaaaat aagaaaaaaa
    23641 gttaaaaata aatcaaaaaa agagagaaat aaaggagcaa ggcgcaaata agcgcctttt
    23701 tatgctatga ttatgaaaac gggaacaaaa ttggttgcca gaggaggggt tatgtgaaag
    23761 aacgtcaaga agagatcttg cagctattga cagaaaacca acaaggattg acagcaagtg
    23821 atgtagctga gcgcttaact atcgatcgaa gcaatgcaag ccgatattta agtgaactat
    23881 acaaggccca tcatattgta aaaaccgctg gccgaccagt cgtttactct ttgccaacgg
    23941 aaaagtcaaa atctgatgaa gtgcatgtcg actcttctac gcaagtgact tttgaaacac
    24001 tcgttggaga aaatgattct ttaaaagtca gcatacagca ggcaaaagct gcgattcttt
    24061 atccaccgcg ggggttgcat acgattattt ttggagaaac tggtacaggg aagtcgatgt
    24121 ttgctgaatg tatgtatcat tttgcaatcg attcagagat gctttctgca gatgcgccat
    24181 ttgtttcttt caattgtgcg gattatgctc aaaatccgca actgctcttt ggacacatct
    24241 ttgggatcaa aaaaggggct tacacaggag cagctcaaga tagtcccggt ctaatagcca
    24301 aagcagacgg cggtatattg tttttagatg agatccaccg tttaccgccg gaagggcagg
    24361 aaatgctgtt tactttcatt gataaaggta tttaccgtcc gcttggagaa agtgcacaag
    24421 tacatgaagc atctgttcag atcattgggg cgacgactga atcttcagaa agtttcttat
    24481 ctacgttcaa tcgacggatt cctatggcaa tcaccttgcc gaatcttgct tcacgttcac
    24541 tggatgagcg ttatgaaatc atctctcttt ttatcaaaca agaagcgaat cgtttgaatc
    24601 aacgtatcga tatcgaaaaa gaagcgattc ttgccttcat gctttatgat gcggaaggaa
    24661 atatcggtca agtgaaacga gacttgaaac tagtctgtgc aaaatctttt ttacattacc
    24721 gtactcatca tgagaaaaag ctaatcattc gaaaagaaga actttccctt caagtacaaa
    24781 aaggattact gaaaatcaag gaaataccgg aacgtctcga ccgattcatg gacagcaaaa
    24841 gtcagtattt gacatttgaa ccaggatttg ctgatgttgt ctggtcacaa gatccagaac
    24901 gaaacatgca ggtctataac gatattgagg aaaaagtcac ttctctttcc gaaaccggtg
    24961 tggagaacat tgatctagag accctcattt caaaagatgt ggatgcttat ttccagacat
    25021 atgtcaaaga attgactaaa agttcgattc ccaaagatct tttgccagat gaaatctggc
    25081 ggttaaccaa tcaattgtat gatgtggctg aaaaacggtt agaccgtatt tataatgaaa
    25141 aagctcgttt tgcttttgct ttgcatctgc aaagtacatt ggatcgtgtg agagaaggtc
    25201 atatgatcgt ccacccagat ttgaataacg tacggaaaaa ttttaagagg gaattccagg
    25261 tagcaatcga tctttcaacg atcattgaag aagaagaaca agttgagatt ccttttgatg
    25321 aaatcggctt tattagtatg tttctttcta tcaatctagg agaaagtgag cctgttaaag
    25381 aaaacaaagt agatgttctc atattgatgc acggcagaca gactgcttca agcatgttgg
    25441 agatggcaca agaactatta ggaacaaaag tagggaaagc ttttaatatg ccactggtta
    25501 tggaagttca aacaatgtat gaagaagtgt tacaatatgt caaaaactat caagagcatt
    25561 tgacgaatgg cctactcttg ttaacagata tgggatcatt aaacaccttc agtgatttga
    25621 tttatgaaga gactggcatt cggacgaaag ctatcacgat gacaagtacc atgattgtga
    25681 tagaagcgat acggatggca agtgttggaa gaagcttaga agatatttat caaaatatcc
    25741 agctttcctt tgaatcgatc gtacgtgatc agttcaaagc ggaaaagtct gaaactttac
    25801 agcgtaaaaa agccgtaatc gtgacatgct ttactggtga aggagtagct gccaaattat
    25861 atcagcgtat tatgccagtg attaatcaaa ataaggtaga actgatccag atgcagtttt
    25921 tagaacgtga atcattcaaa aaacacatcg atgccttatt ggaagaatat gagatcaaag
    25981 caattgcggg aacagttgat gttgattatc aaaatatccc atttttttca gcctatgacg
    26041 tttttgatga tgaaaaacta aatattttaa agcgtatagc tgatgatgag atcccgacag
    26101 ctaaaattgt cgattcactt gctggatcat taagcgatgt cttttctatt gatgaactga
    26161 tccgttcatt gcaaaagaca gttcagcaga tccagaccga tttgtcgatt atcgtggaac
    26221 ctggagtaga tactggaatc gttatccatt tagccttcat gttagacgga ttgatcaaac
    26281 aagaaagcca tcggcaattt gagaaactgt ctattttctc aaaaatttat cgtttggaaa
    26341 tggatatcgt tcgggcaaac ttgatggcac tagaacgaaa atatcaggta aaaattccag
    26401 aagatgatat cgcatttctt acacaaatgt ttcttgaaaa taaaataaaa aaatgaacac
    26461 actaatcata ctgtttacac actaaacatc tgtgtgcgca ctataaaaaa gagctaaaaa
    26521 gcatgaaaac gctatttcag ctcttttttt tattggcatg cttcttgcgt tatataaaag
    26581 tgtaaaaaaa taaaaggagt gttaaaaatg gcaaaaaaaa caatcatgtt agtttgttca
    26641 gcagggatga gtacgagttt attggtcaca aaaatgcaaa aagcagcaga agaaaaaggg
    26701 atggaagccg atatttttgc ggtctctgcc tcagatgcag ataataattt agaatctaag
    26761 aatgtcgatg tattattatt aggtcctcaa gtacgtttca tgaaagctca atttgaacaa
    26821 aaacttgcgc ctaaaggtat tccattagat gtcatcaata tgcaagacta tggcatgatg
    26881 aacggagaaa aagttcttgc acaagcagaa aatctaatga aataaatcaa gtatgcagtt
    26941 gggggtataa caacgtggaa gatcaacaaa atctagaagc catcatgggg ttaatcatgt
    27001 ttggcggtaa tgcaaagagc gatgcaatgg aagcgattgc ggctgctaaa atcggtgatt
    27061 ttgagttagc ggataggaaa atcgctgatg cagaagaatc acttatacaa gcgcatcatg
    27121 cacaaacaga catgttgaca caagaagcac aaggaaatca tatgcaagtg acattattga
    27181 ctgttcacag tcaagatcac ttaatgacat ctattgcgtt tactgattta gcaaaagaaa
    27241 tcatcgaact ttatcgtcgc gtatacagcg aataaataca acttcttttt tgaacggaag
    27301 aagaaaaatt tttgaacgga ttttttattt tgttataagg agaactctct ttatataaaa
    27361 aacttatagg aggaagtaaa aaatgaacgg actatcaagt tggatggaga aatacatcct
    27421 gccggttgcc gcaaaaatcg gagcgcaaaa acatttagtt gctttgcgtg atgcttttat
    27481 cggcacactg cccgcaacaa tggcgggatc agtcgctgta atgatcaatg caattattcg
    27541 tgatttaccg caacagttta acagtaccta tgctgacagt ttagcagcag acaaaggaat
    27601 ctttgccgta ttgaatgtca tcatcggaat caatggtttt gtctggaacg gtacactagc
    27661 aatcgccgga ttgatctttg ccttttcttg ggggtataat cttgcccgag cttatggagt
    27721 aaatgatcta gctgggggga tcgttggttt agctaccttg attcaaggta tttcctttgg
    27781 tccagcatta tcagcagcat tagatgtgaa tgttccttca aatgtcgcag atgcaatcaa
    27841 tagttctgag attggtgcaa cgattgcaga tggcacactg tcagttggat tatggggttg
    27901 gctaaaacta gatcatttga atggaaacgc ttattttaca gttatgatca tgggagctat
    27961 ttcagtcatt attttttgta aattgatgct tgccaacatt acgatcaaaa tgcctgattc
    28021 agtaccacca gcagtatcta aagcatttgc agccatttta ccagcgacga tcgctttata
    28081 cgtcattgct attatcaatt atatcgtagg tcgtgtagct gatgggcaac tgatcattga
    28141 tttggtgcaa aaatatattg cagaaccatt cctaggatta tctcaagggt tgggagcagt
    28201 attgatcgtc acagtatttg ttcaagtatt ctggttcttt ggtattcatg gaccaaatgt
    28261 attggcacca gtgctagagg gaatctgggg acaagcacaa ctaatcaata ttgatatttt
    28321 ccaaaaaggt tataacggaa aaactggtac accagccgtg ttagatgcaa tcgaagatgg
    28381 aaaagcttat atgtgggtcc gcggatcgtt cgatgctttt gcatggtttg gcggatcagg
    28441 cggaacgatc gtcttactga tagctatctt gattttctct aaacgtgcag attatctaac
    28501 agtagctaaa ttatccattg ggcctggtat cttcaatatc aacgaaccag tgatgtttgg
    28561 tctgccaatc gtattgaatg caatcatgtt tatcccattc ttgattgctc cagtagtagc
    28621 aacagcaatt ggatggacag ctacttatct tggcctagtc gctccggtat cgcagcaagt
    28681 cacatgggta gtaccgccgt tcttattgtc attcttagca acaggagcgg attggcgggc
    28741 accgatcgta acattagtat gtatggtcgt gactttcttg atttgggcac catttgtcat
    28801 ctctgcaaac aaaatggacc ctgccgcagg cgaacaacaa taagatgaac gtgaggagcg
    28861 ggacgcttta agtttcgttc ctcataatga attttctagg aggaactgaa atgattacaa
    28921 ttgccagtgc aatgcaacaa gatgaaatca aagaattgtt agaaggatac aatgaagaag
    28981 gaatgacttt tacatttaaa gaaaaacaag gaatcaagct gttctttgaa acaagtgcag
    29041 aagatccgga agttgcggct aaaaaagcaa aggaactaat caaagcacag ccttggggaa
    29101 cagtccttta ttttcaatca acagctgttt agaaaatggt ttaaaaagat aggatggttg
    29161 agatgataaa aaacggttta tttttatgta gtattggttc tttgatcgtg ttgtatagtt
    29221 tgaatccaca ttcgcaaagg tatgatttgt atagtatggt cacaggtctt tttctaacag
    29281 gattaggaat ctatttcttc atgaagggga aaaaaagaga agaagcaaag gagaaaaata
    29341 actaatggaa atcacgaaaa aattaaatat tccagcatcg tttttttacg ataaagttat
    29401 ggactctgtc cttttcgatg tacgtaaagc gaccggcaaa tcagtaacta gaaaacagct
    29461 gaaaaatttg gagtacgtca aacaattctc gcaaaataac cgggctcgca taaaaatcga
    29521 agaagtcatt gaaaaccagt cttataagtt tcgtacatcg acaacgaaga atgaattcgt
    29581 cgttcattat caaatcaaac cattggacga ttcgacctgt gaggttcatt acacagaaaa
    29641 aatggagtct tatggcatgc ttcaaaagtt gaatgatatg cttttaggaa caatcttgat
    29701 gtattttaaa cggagacgtt tcaaaaaaat gctagatatg atcgaagcaa cgtattaaat
    29761 caatgaaaca tggtactatt tagttattga aaaatgaagt aagctagaag gaatttttca
    29821 taggaagata gatctttcct gcgaatcctc actagctttt ttgtaggaag gggcagcaaa
    29881 atgacaaaca aagtaagaga gctaggtgta tcttcgattg aggaaatgtt tgatttagcc
    29941 acttatgcat ttaatggggc agatacacct gaaaggcgag aacgttttcg tacacttgcg
    30001 gagaattctt ggaattacgg attttttgat gaggaggatc ggctaacaag tcaagtcatg
    30061 gcgacccctt ttccagtgaa tttttacgga caagagtacc taatggccgg cattggctat
    30121 gttgcttctt atccagaagc aaggggacaa ggcgggatca atcagatcat ggaaaagatc
    30181 cttgaagatt gccgtgatcg taaagtaagc ttatcttatc tggcgccatt ctcctatccc
    30241 ttctatcggc gctatggcta tgaacaatca tttgataaaa tcagttatca actaaacagt
    30301 cgtgatgtgc cgtttatcaa aaaaaaatct ggaaaaataa agcgtatgga ttttgaagat
    30361 gcaaaagctg caatcagaag aatttatgaa cagatgccgg aaaaccaacg agctggtctg
    30421 aagcgcgaag actggtggta tacgtataaa ttcaagcaaa aaggcaatta ccagtttgct
    30481 ttgcattttg atgataatga ggaagcaacg ggatatatcg tttatcagct tgcaacgtct
    30541 tcctttatta ttgcggagtg gggatttttg gatcatgatg ctttttgcag ccttgttcgc
    30601 ttcgtaagtt ctcacaatgg tgcttttgaa acatttgaat attcatgcgg ctacggcggt
    30661 aatgatcaaa attatctatt agaaaatcca tttgcttctg taatgatcac accttatatg
    30721 atggcgagga tcgtagatat ttctttgttt ttggaaaatt atccatttaa aaagagacaa
    30781 gctgcatttg ccctacaaat agaagaagat cattatgcga aatggaatga aggaatcgtg
    30841 gaagtaacaa tcgaaaatgg aaaaaatcat ctctcaaaag tagaacaaac aaaactccca
    30901 atcgttcgag gaagtatcca acatatcacg cagctattat tagggtatcg taaagtggaa
    30961 gagttggtat tccaagaaag aatacaggtg acaaaagaat atgaagaaac gttaagctca
    31021 atcttgccac agcaacagcc aattcttaat gactattttt gataaaaaac gggtgatgac
    31081 aatatttttg tcaccaccct tttgtttttc atttggcatt tgaaggaaaa tgttaaagta
    31141 aatcctcttt tttcgtaaat ttgaattttt tgttttcttt tgttttaggg ttttcttttt
    31201 tgtggtcttg gattgcttct ttcgtaatct tttcccgatt ttttttagat gaatctaagc
    31261 gttctgcgta tactttttct tttggcttat cattcattgg aaaggacctc cctacattat
    31321 ttcattctac ctctagttta tcatctgtta aggactagtg gaaacgtttt gcctaactta
    31381 taagctgatt tttttagcag ataagcttgc aagtaaaaaa caaacaagct acaattgatt
    31441 tattgaaatt gttcctgtag ctcagtagga tagagcaatc gcctcctaag cgataggtcg
    31501 ctggttcaat tccagtcagg aacgtataaa taacccctgt atcctttgcg gtacaggggt
    31561 ttgttttgta tttgtgccaa aaatgtgcca aactagttat catttttata taaactatcg
    31621 aacacagtat tgattttttc tttatcctga tcttcaagct ctcgaacaat atgaagataa
    31681 gtagacattg tagtttctaa actgctatgt cctaaacgtt tcgatacgct aagtatattt
    31741 actccttgat aaagtaaaat agatgcatgt gtatgcctta gtccatgcaa agtaaattgc
    31801 ttttcaatcc ctaattcttt taattttttg cgcaaatatt tgctgacggc gtttgatgaa
    31861 actaatccat tttttaagtt gaaaaaaaca aaattatgag ggtttttgat ttcaaaattc
    31921 tcgaataatt ctttttgatt gattttaaac ttttttaaga gatcaatcgt atgtttatca
    31981 atggaaattt tcctatttga agtttcgttt tttgtttttc cccattcatt taatttataa
    32041 ttccatgttt tattaatagt gatgatttga tcttcgaaat cgatatcttc ccatgttaat
    32101 cctagaagtt cagcaaatcg cattccagtg gcaccagcaa ctaacacgag cataggagaa
    32161 gagtactgcg cagaaaggtt ttcttctacg agtttcatta aagatttaaa ttgatcataa
    32221 tctaaatatt tatcttcttt tggcttcaat gaatcttttc ctttgattac tgcttttcta
    32281 gttggatcaa acggaattaa tccttcttct acggcatctt tcaacgacgc tctaatatgg
    32341 ttattaaact taataacggt agattttaca tggtctttag catacttatt taaaaagcgt
    32401 tgatatccca ctctatctaa atcagaaatc aataccgctg gcatatattt ctttatgttc
    32461 attaacgtat cttcgtattt tctataagtg ataggagata ctgttccttc tttataaagt
    32521 tgcatccaat cctcaaagta atctgataat aatagatttt tgcgatcccc tttaaggcct
    32581 ttgttcaagt tatattctaa ttcgttggcg gcatcttttg cttctccttt tgtcttaaat
    32641 cctgattttc ttatcttcgt gtattttccg tcatcttttt tgtatgaaat ttcgtattgc
    32701 caactatttc cacgtttgac taatcttgcc ataattgaat cagctctctt tctctgatac
    32761 aataggaact ataaagaagc ccattgtttg ggttttatta ttttttgcac atcctttttg
    32821 cttgccggcg caaggatgtg ctatttgtcg ttttaatggt ataattggat gtgtattaga
    32881 tcatccaatc agaggtgttt attatgatta aagtgatttt taaaagaata actgaacttc
    32941 cagagtttag attatttgtt gattattggt atgtctggtt ttttattgtt gcattgctga
    33001 taggtctaga tgtcatatta gaaagaaaac ttaagtaaga gttgagttaa taaattgaat
    33061 tcctaaatga gatattttgt agcccatatt ttgtttgccg acatcaccta atactatgga
    33121 gctaatcgtt ttttctaaca gaccaaaatc agataatcta tttaagctac catctattag
    33181 ctcttgtgaa tagccagaca tgctgatatt tccagtgaaa ttttcgtttc cacgactcat
    33241 taagcatgct aacaattgaa tctcaatcga tgttaattga ttcagtacaa gaagataata
    33301 ctcctcgtta tcccatgaag cgctgttaat tcgaagtaaa caattcttat aaagattttt
    33361 atacatatct attttatttt gtgcctttga tttttctacc tcgtcattta tcccttcgaa
    33421 tattccaatg agttggtcct tgttctctaa gttgaaaaca tcttctggaa tttgatcgcg
    33481 aatgttttct aatgattcgt ttaaattttt gtagaatttc tcgatgcgtt tgaaacgctt
    33541 ctcgttttta gacccaaaat aaagtgactg cacaacgcca ccaatataag gaattgctcc
    33601 aataccgcct tcaactatta attcaagata ttcttttttg gttagttttt cattgctatt
    33661 atccatagtt acctcctatt ttgtcatttc ctttacaagc attgtgactc ctgatggcac
    33721 tcttttgtat aggttttatt attgtgcaca atctaactct tagtggggcg tggttcattt
    33781 attttttact tactttacta ttaatcatat cccattgagc ataggatttt tctatgttcg
    33841 atcctcttga actaatttct cctgtaaaag ttactttatc gccctttgta aaggtatcag
    33901 cattgtttaa gtccttcgca aaaattacat atgaaacttt aggtgttgca gaaatatcat
    33961 tccaactttt atcatcgtac tctttgtctg ctataatggc tattctagta gtcattgatt
    34021 cgataacagt tccttcaaaa gtatatgttt taccactaat atatttagta aaagctgtat
    34081 tttgctcagt agctggagta atagaataat aactatctaa gaaagcaccc cagtcatgat
    34141 tatttgtctc taaaaattct tcaaattgag ttaggctttt attgctgctg ttagatgtag
    34201 tagtttgagt atcagatgat gagctttcct gtttgttatt gtcatttgcc tcgtttgatg
    34261 aggaaagcga gaaacctacg gtagtaaaaa taatcccgat aataaggaga atcataggtg
    34321 ttttcttatt tgtttttttg ataaatctta caattaacat gattaaacct ataataaata
    34381 aaacaatacc agtaaaacca aatataccgt ccattaatag atcctctttc tcttttgata
    34441 ttgaatatta taaaccaagc aattcttttt tcttaaaatc gaattcttct tgattaataa
    34501 tcccttcatc taacaactct ttatactttt taatttcatc tgcacttgaa atttgtgttt
    34561 ctttggaatt attatctaat atagattgtt gcttctctat atattttttt aaattttcca
    34621 tttcattcca atattttttt gtaaacataa cggtgttttc atctttcgtg gccgctaata
    34681 tacctccgcg actttcgttt ccgcctagca aggtaaattg tatataacca ttactcatac
    34741 ctggtttttt taattgtacg gctgatatat ttttaaaggg gatagttttt tctcctttaa
    34801 taccttgatt aactaaattt ataaatcctt ttctaataat tgaaatagaa tcatcagtta
    34861 ctttaatcaa ggtttttcct ggagatttta ttaaaatctc tttttccatt ttttcttcct
    34921 catttctgtg atatgatttt ttttgtagaa gctcatatat gaggaaagag cccgtgttgc
    34981 agcacgggct tttttaatgt tttgctgaaa tcggtttttt taataattct tgatgtattc
    35041 atacatattg ccttgcgtaa gaatattttt tcttaaaata gcattggcag acagcataac
    35101 aagtgtatct gagcttattc tgaatagaat aatactccat aaatttttcg agattaaact
    35161 gagattcatc agtcagttcg ttctcaatat agatatttaa taaaattaga atagctattt
    35221 tatctgcttc tgtttcaaat tttgagtgaa aagttgtaga agtatcgtac aaaactgaaa
    35281 attcaaaatg agaggcgctg aaatgtgcaa gttcatgaga taaatgaaag gcttctgcag
    35341 tttcaccgta tagattttca ttcaaaaaaa tgattctggg ttttggatag tagaaaccag
    35401 gctctttcat ctccatataa actactttca aattgtattc gctcaacatt tctttcaatt
    35461 tcaaatacat acaaaccatc actccaacta ttcattttcc tctaaagctt tagcaattgc
    35521 aatcgcttta cgcattgtct ccttagatat ttcttttcca tcaaaagaaa aaacagtatc
    35581 gtcttctgat aaatccacat gtttaggggt ttctctttct tctctaccta gaaggtagtc
    35641 tacagagaca tcgaaatagt cagcaatccg acttaattca tctgaattag gagtattatt
    35701 tttccacttt gctagataac cgttagaata tccaaaatta atttctaact gccgtataga
    35761 tatctttttc ctcttcgcca agtcttttat tatttcatag gtattcatcg ataaaacaac
    35821 ctttctaaat gtttacaaaa aaagtttaga aaaatacgca gaaataattt gactaattct
    35881 gagtaatgag ctatactata tcttgtaaac agatttaaca actaaaaaga caacaaaaaa
    35941 cactattgat atgtaaatgc agaccgccaa gaaagcttta aaatcaatgt ttttatgtct
    36001 tatttaatta tgctcttagt atagagtatt actcagatac tgtcaattga atttagaaaa
    36061 aagttgttaa atttgtttac gaatataaaa gaaaggaatg agaaatatga acacaccgca
    36121 aatttttaat ttcgaacaaa acgaagttcg gacaatttta gtaaatgatg aaccatattt
    36181 tgtaggcaaa gacgttgcga gtgttttggg ttattcaaac actaaagatg ctttgtcgcg
    36241 gcatgtagat ttggaagata agatggggtc gcgaatcacg acctcaggtc aatcaagaga
    36301 gatgacaatc atcaacgaat ctggtttgta cagtttaatc ttaaaatcaa aacttccctc
    36361 tgccaaaaaa tttaaacgtt gggtaacaag tgaagtgttg ccagcaatta gaaaacatgg
    36421 aggttatcta actccagaaa aagtagaaga agctttgctt aatccagata caatcattca
    36481 attagcaact caactaaaag aagaaagaac tggaagatta atcgcagaac aaaagattgc
    36541 agagtacgaa cctaaaatct cctatttaga tagcatatta tcttctacag attcagtaac
    36601 aattagtcag attgcagcag attatgggat gtctccacaa cagatgaata aattacttca
    36661 taaactaggt attcagaaaa aagtcggtaa ccaatggtta ttgtgcaaaa aacacatgaa
    36721 ccaaggatac acaaaatctc atacaactga gatcccgaaa gccgatggtg gcactaaaat
    36781 tgtaatgaat accaaatgga cacagaaagg gcgtctattt atctacgaat tactaaaaaa
    36841 agaaggatat taccctcaaa tggatttaga ggaaattggt tagaaaggag ttttagtatg
    36901 actgacattg cagaaatcac tcaacgagat agagaaaaaa tcaaagaata tgtcgaaagt
    36961 tcaaagttct taacttacac catgcttgct gaaagatttg gaattagcaa aagctactta
    37021 tctttaattt taaacggtaa aaagacttct gcagaagcaa acagaattat agattcgatt
    37081 atcactatgt acgaattgta aagggaggaa cagctaatgc aatatctaga agcaaaaatc
    37141 ccaattccag aaggctatgt aattatctcc caagtggatt atgaggagtt aaaaaaagct
    37201 gatgatactg gtagatggat gacgttgcca gaagtgctag aacggattaa cagaaaatat
    37261 gattggttta cttctagagt tttaaagaac ccaagatata gaaatattat cgatatcgag
    37321 aaaaacaaaa atggatttgt ctattatcca gttgaaggaa gagacacata tctattttta
    37381 agaagtaaaa cacttgaatt tttagaaaca aatttttcgg aaatcttaag gaggcaagcg
    37441 gatgggaaaa tttaatagag cgctagtgtt cagcgcaccg ctaatcatct acgctttagg
    37501 actttgggga agcaggcaag cgttgatagg aacgattgtt tacatggttt ggatttttat
    37561 ggggcttgat gaagctgaag ctgagtacag agcgaaaaag ccaaccgagg gggctgacta
    37621 aaaatgaata gaaaagagaa actagaatgt atattactat tactcagttt aattctttca
    37681 ctaatttctc tattgggaag tttgtatttt tgatatcaac aattttgtgg atttgggttc
    37741 tatttagagt aaatatcatt tttgtttggt gattaaggag tttattaaaa ttaattttcc
    37801 ctacttcaaa tgcaaggtaa caagatatag cacttttagg aggaatattt agcggtacag
    37861 cttcagagta tattctatta tcatcgactg atcctccttt agcaattaat ttcttaaatg
    37921 gagtagctga ccatctgcgg tttaagttag cggatgaaag ttctaaatta actaacatag
    37981 ctggttctgt tgaatagtta gaaatgatta cttttgtata aatcatatct gcagcgaagt
    38041 aagaagcatt aagttcaact tctagttgag ggcgcattcc ttttattttt atccctgtta
    38101 aaaacgtact caatataaaa cttattattg acatccattg aaaaattgtt aagtgaaaaa
    38161 aattcaaaat aaacaccacc agtttttaac taaattatac caaaaaggag agaagaaata
    38221 atgcaagaat tagtaatttt gaaaaataaa gaagctgtga ctacgagctt acaagtggca
    38281 gaaagcttcg agaaaaaaca tcaacatgtt ttaagagata ttgatgcact aaaaaaagat
    38341 gtgtccaatt ttggacagat gtttgtagaa ggtaatgaac cagattcata tggcagaaat
    38401 cgacgagttt tcttcattag tagagatgga tttttcttgc tggctatggg gtttacagga
    38461 aagaaagcta tctacttcaa acaaaaatac attgaagcat tcaacgaaat ggaagatgtt
    38521 attcgcaaga atactgttcc tcaaacaatt gaagatatga tgatctatca attagaagaa
    38581 atgaaagatg ttaaaaaaga tgtttccatg cttaaagata ctatgcgaat tagcggacaa
    38641 caagagtttg aaattaagca aaaaggaaat atgaaagtta tgggagttct agggggaaaa
    38701 gaaagccgag cttatgaaga aatcagcaaa aaagtattct caaaattttg gtctgaattt
    38761 aaacgtacct tttcaatccc aagatatggc gagttacctc gtaagagatt cgatgatgct
    38821 gtttcattta ttgaaatgtg gttgccagaa actgcgatcc gtatggaaat tgatcaactg
    38881 aacagacaac agagactttt tggtgatgac aatgaataga gctgaagcgc taagaatagg
    38941 gacggcaatt gctaattgct ggtggaaata ctataaacca atcatcctaa gccaacaaca
    39001 tattgacaag caaaaagcat ggcaacaaat aaaaaagtga ctccgccggc aagcaaagag
    39061 tcacaaagaa aacacatcat aaggagattt tagcatatgg aaaaagaact ttccactcta
    39121 gatcaatatt tgattgatcc tgattggggc aagccgaaaa ttgaggaaac aagtggtcga
    39181 aaaatcagac gaaatctttt gacgaatgaa gaactagctt gggatcaaga tgatttaggc
    39241 aaccatgtaa ctatttggga tcatgtttat cttatccatc tatcgaagca ttcgaataaa
    39301 cctgaatata tttacgtcat cgaagatggc ttgattgatg cgctagaaga gtacgacaga
    39361 gataacttga ttgatatctc ttattacgga ccaggtaaga aatacattgc tgaaatggag
    39421 gcagaatttg atgagtgaaa tcaaagggac aacgaacttt gaaaaacttt ttagtcgtaa
    39481 gttaaataaa attctcaaga aaaaaggaaa ttttgattat ttatcttggg ctcacgcgtg
    39541 ggagattatg aaaaagaatg atccacaggc aacggtaact attaatgagt acaaacacta
    39601 cagggttgtt tctggaactc atcaagactt tcttgttgag gaatataaac cttttcttat
    39661 ggatgaaact gggacttatg tatctgtctc agtaacggtt aaaggacaca cggaaaccga
    39721 attatttcct gttttagatt atcgaaacca accagttgtt aaaccaaatg caatgcaaat
    39781 caataactca ttgaagcgat gctttgtgaa agcattggct ctacacggac tgggattata
    39841 tgtatttcaa ggggaagata ttccaacacc acctagaatc gatacaaaga aattaaacat
    39901 gctagagacg attctagaag ctttcaatga gcagatgggt aaagatatga ccaaaacctt
    39961 aatcgaatat gttaatgagc agacagataa attagggctc ttagctgata acgttgaaac
    40021 tattgaacag ttaagctatg agcaatgtgc cttgatggag cgagcaatag cagctaagag
    40081 aaaagaatta gataagaagt gatatgagtg tttaaaccat taatcgattc atattcagcg
    40141 gttctgaaaa agttcaaagg aaaagacata agcgcaacca tcaatgagga agtgaacatt
    40201 gatcgactaa agacgatgta tgacggctac gatggtgatc gagtcattga aattcgtttt
    40261 attgatccta gacgtttcac cgtacagcaa cgaaacttca tctatgcgct gataggcgat
    40321 atttttatcg atacaggcat gccaacggac ttctggaagg aattcttcta ctttcgtttt
    40381 gaaggtgtca cagggcgcaa aataagcctg aaagacgaat cgaatacaac tgtgagtgat
    40441 gccaatgtct tagcaaatat catcttagat ttcatctttg aacatcatat tcctttcaaa
    40501 gaaggctatg agattttacc tgcgaatcaa gaatattact tctacaaatg catcacaaaa
    40561 agagtctgct gcatctgtgg caaaacagga gctgacatcg atcactttga caaagcgcta
    40621 ggaagacgaa agcgcaaaga agttgatcat gcagagtaca catttgcagc actctgcaga
    40681 atccatcaca cagagaagca caaaataggt gtgattaatt tcaaaaataa atatcaaatc
    40741 aaagggatca aattaaacca gaaaacaatc aaaaagttaa atattggagg gtaaaaatga
    40801 cagaacatcg aagttattat gcgattatac cagccaacgt aaggtacgac aaaagactta
    40861 aaccaaatac taagttgtta tacggagaga taacggcctt gtgtaatgaa agaggctttt
    40921 gttgggcagg caatgagtac tttgcagatt tatatggtgt gaataaagag accatatcgc
    40981 gatgggtaag tgatttgatt aagtttggat acttgaatcg ggaaatcatt tacaaagagg
    41041 gtaccaatca aataatcaat aggtacctac gaattaatca ataccctatt gacgaaaaac
    41101 gcaatacccc tattgacgaa aaagtcaaag ataataatac atctattaat aatacattta
    41161 ataatacaaa agaatatata agagagttac cgccttcgaa aaaatcgaag gctaagcccg
    41221 tccgtcataa atacggagag tataaaaatg ttcttttgtc agatgagcag atggagaaac
    41281 tcaaaacaga attccctaat gattaccaag agcgaatcga acgactgtca gagtattgtg
    41341 aatcatctgg taagacttat aaaaactatt tggcaactat tcgaagttgg gcaaggaaag
    41401 aaaaaagtga acctaagaac gcaagcagtg gatacaagcg cacaggaaga cgagagaagc
    41461 ttcctgaatg ggcaatcgac caagaagcct atctcaagaa aaaagcgcta gaacgagcta
    41521 atagacaatc aaaagcacca ttctaagagg tggaaaaatg aagatcgatt atctagaact
    41581 aattaatgaa atagcgaatt ataaaaaggg cgaggaatta gacgtcctga gagacgtata
    41641 tgatcaactc gaagaagctg gaattgaagg aattaagaat gatcgttcga gttggagtaa
    41701 gctcagatac tatttcgcac tttatatcga tacaacacaa ttaagaaatt tagcatatac
    41761 caaattacta tttgttgatt gtgttaaagg cttgcaaaaa catcttaatg aacttgagca
    41821 ggtgtaatca gatggaccta aagacattta cagcacagat tgaactaatg catcaagaag
    41881 ctttaagaca aagtgtatcg tacgaagaca agtggctcaa cacgttccat ggcggacgtg
    41941 agagcgcact tgatcaagta ctcaaattac tgaaaggaga atgtcgggat ggataagaaa
    42001 gcggcaatgc agcgaattat cgaattgact tattcagaag attggcaaaa tgacaaagaa
    42061 gctgcttcag aagtgatgag gcttggaaga gcgatgtggg cagacaagag caacaagcca
    42121 agaccacgaa aaatcgcaat ttggcacggt gacaaacttc tagtgatagg gacagctgaa
    42181 cagttagcaa gtctcacagg cttgcacgag aaaatcgtga ggaaaagagc aaggtgtggc
    42241 tacacagacg ctaagaagag aacgtttaga tacgtggagg aatcatcatg acaacagaag
    42301 aagtgattca aatgcgtatt cgaagccttc agcgtgagat tgacgatctg gaacgaacaa
    42361 aggcagtgat ggtcaatgaa acggcgagaa aggcaatcga tttgcacata gagaatttaa
    42421 gaagggaaat ccatcgattg gaggaatgag cgtggataag aaagcagcaa tgaaaagaat
    42481 tgctgaatta accaagtcag aatcttggca agaagacaaa gaaatagttg cagaagtcca
    42541 aaagctcggt aaatcaatgt ggactgaaaa gcccaaacgg agaacgccga gaaaaattgc
    42601 aatctggcat gatgaccgaa ttttagtaac aggtactgct gaacagttat ctgaaattac
    42661 tggattaagc aaaaacatta tctgggatag agctaggagc ttatggattg attcaaaagg
    42721 acgacagttt aggtatgtgg aggagagata atggatctca ttacacaata cagtgacatc
    42781 atcctcaaga aaatcatgat gaagattcag aaagataaaa aatcaaaaga acgagctgaa
    42841 ttagttaagt tagaaatggc tgaaacagga gcaggagtgc gaagtagcag gcattggaaa
    42901 gcagcagcaa acattgaatt ttattacaac gaaattcaaa aagggttcga tcagatgcgt
    42961 gagctggatc ggcaaacaaa ttggagcaag aaacttcatc aagatcgttt caaatttgta
    43021 gagaagtata aagaaatatt agaagagtat ttgaggagga cagcaaatga taaaaaaact
    43081 cgttcaattc agcatggatt tatatgatat cgaatcagga gcaacactat ctgtggaatc
    43141 ggaccatcta atcataaatt ttggtggaaa gcgccagatt attttgtggg tagttgatga
    43201 tgtactgttt ccagaaattg ttcatgattt cgaagaatca aaagcggttg agtttgaaat
    43261 agtgaaaaaa gtaatggaat tgattgaaaa atacgaggag gacagcgaat gataccgaag
    43321 tttagagcgt gggataaacg aaagaacgta atgagagatg tagccgtctt gcattttact
    43381 aaaaacggca aaacaaactt tattgaatat tggataaatc ctaccgaatt gaaatcatat
    43441 cacgtgcgaa acatcgacct catgcaatcc acaggaatga aagataagaa tggtgtggat
    43501 atatttgaag gggatatagt attagtcagc gtgcgaaatg gcttcgatta cttagataat
    43561 aaagtctgta ttgtcaaaaa ttcaatagat tattccggat tagtttgtgc cactgttgat
    43621 gaagacttag agtatcaaat ttttaacaca gagctgtttg aagaatacac gtacgaagtc
    43681 accggaaata tatacgagaa tagcgagtta ttggaggaac agcgatgaat aaacaggaag
    43741 caatcaaaaa gttagaaagt attaaagcga taggaaatga tgcaatagct gcttgctata
    43801 acgagagtat aaattcaggt attacgttaa tgaaaaaaat agacgaaccg cagaaaccag
    43861 ttattccaca actcgtggcc ggttggcttg agaaatctac ggaccctttt acaaaagctg
    43921 aaaaaatagc gtatttaatc aaatctaaag atggtgattc atattatttc tgtgattggt
    43981 ttgtacgaga tggcatagtg acgcaagagc aaggagaaga attactcgct tgggcaacaa
    44041 gacaatcata tgaaacacta ttgagcctat acaacggcta cgaggttgag aaagagccgt
    44101 tatgggcaat aaagaatgcc gatggaaact atcttactaa atgtgcttta tggggaaaag
    44161 atggagtaaa ttatagtttt gaatgcaatc catctcatcg attgcttttc actgataaag
    44221 caacagcgga tgctgcagca ttgttggtga atggaacagt ggaagaggtg gtagaaagat
    44281 gaaactaaaa gacggatttt acgctagcag tcacggtatc ggcggtttaa tgctagatat
    44341 gccgacaaag aaccctaaaa cacgcaagaa atcaaaattc aaagtcggtg acatggtccg
    44401 ctgtgaagca gaagagttcg tttatccgtt cagaggatac gttaaaaaga tactgtcaaa
    44461 ctcagcaatc attcgtattg aaaatacaat gaagtgtgat cgttcgactg caaaacacaa
    44521 gcattattta gctgtagcga gattagttga catggaagta atcaagagca aataaaaaaa
    44581 gccggatcgc tccgactgat tcaataaatc caacacattt attatatcac ataaaggagc
    44641 ggtttgactt gatgcaattg ttacgagagg tagatttcaa acagacaaga tgtaatgcga
    44701 gagatgtgct gaagaacttt cggcgtttgg agcggatggc aggtcgctct ttgatagata
    44761 ttaagtcgcc gattataacg gatatgccga aggcaccgaa gcatggcaat aaggcagaag
    44821 acgcgattat tcagatgatg gatatagaaa cggagagaga tgcgattcta gcggctttga
    44881 tggctcttag tctgattagt cgtcagatac tctactacag cttctgtgtg ccagatagct
    44941 tctcaaacta cagaattagc cgtgaagtgg gttattcaga aagaagtata caacggatga
    45001 agtcggaagc tctaatagag tttgcagaag catataaaca cggaaaaata attgcttata
    45061 aataatttgg cggttttttg gcggaatgat ggcggttttt agacatttac cagtgttatt
    45121 atggtagtgt cgaaagatta ggaaacagga tcgacaaaat aaacgaaggg aggcaatctc
    45181 cctcatcgtt ttaaacttct tcttagatag acagcaacta attatgaaga aaaggatgtg
    45241 aattcaactc ctcctaaatt cttcatcggt tgctgtttat tttgattaga cagcagcact
    45301 cacaaaacta aacatgttct tgtcgtcttt caactgctgc tgtctattaa tttatgtatt
    45361 ggagggaaga aaaatgaaat tatcagtaga aggcactcca gaagaaatac aagaattgct
    45421 ccaagctatt ggtagtagca aggagcaaaa ggtaagtgtt tctaattcag atattgacgg
    45481 tatttgcaat tgtttaaaca gagaaaaaat atctaatcct tactaagaga aaatatgttc
    45541 gttaatgaat ttccaatttt cctctgtagc tctaccttga taattttttt gcagttcaca
    45601 gatgaataag gaatcgttat tatcaagtac tgtatttaat ttttcacaca tttgatttgg
    45661 attcaaatta gttttaacta accaaaaaga tttttgtagc tttatatatg ccccaaattc
    45721 ttttattatt tcaaatactt tatcatactt ttgccctggg ttattaagat cataactaat
    45781 catgtagggt ttatacatgt tgtcttgcac atccttgttt tatatttcag cggaccactc
    45841 gctgataaat aaaattatac gcttagtatt tattttcaca atattaattt atcgtaataa
    45901 cttttgagta aatagaacaa aaaacctgca ctagtttccg ctagtacagg cagtgactat
    45961 atcggttgat aatctagcat atattaaaaa taattgcaag aaaagaaatt tatagtggtt
    46021 tataccaaat tatcaaagca atgttatttt gttgctgtct attgtttttt aattattcac
    46081 acgataacta aaggtgggtg aagagaaatg attccattaa taatttcaat ttttgcgctc
    46141 tgtcttaatg tctatatgat tggatttaaa aatgggcaaa ataaaaaata gtagcagcca
    46201 agaataattt tatagtgtca ctgtggcgga aagggtagac gctaagcatg tgtgctaggt
    46261 caatgcttcg gcaaccatgc aaggttcgat tccttgccag cgacattaaa tgcctatgac
    46321 ggttacgact accgaaaaaa gatcgttaag aagctatacg gtgctacgta cggcaatgta
    46381 gtaagtgtgc tatctgtaca ccaccaagct tcggtcactg tggcggaagt agaagacgca
    46441 gcggtaaatg gcgagtagcc tcgtgagagc ctggtaagtt ctcgtgagtg gtgcaatccc
    46501 actccagcga ctttaagcaa ccgaggcatc ggcggtttaa aaatataggg gtgcgcaatt
    46561 tcgtacgcgt tttgtgcatc gtgcaagtta ctattacata ttagatcact ctttgagtgg
    46621 tctttttatt tttgcacaaa ggaggaaaca acaatgtata gaccacaata cttagaacag
    46681 aagtatgaag taatcactgt tcataaaggt aatggcgaaa aagtatatga gtatagaaga
    46741 ccaataaaga gcgatacata taaacgaaag gaaaacaatg aagttattcc attgtatggc
    46801 aaaagaatag ctaagcatta aataagattg cgaaaggaga cggaacatga ccgaggaatt
    46861 ctatagatgg ctattacagt tgacaagaga agatcgtttg gttaagttct atcagtctcc
    46921 taaatggcgc aggcttagag agaaagcgat gaaacgagat cactatgaat gccaagagtg
    46981 tagaagacta ggtaagtatc atagagtaga gaacgttcat catataaagg aagtcaagga
    47041 tagacctgac ttagctttag atttagataa tcttatttgt ttatgtgttg aacatcataa
    47101 tgaagttcat ggcagatatc ttacagcatt agataaacaa gagaagaaga tagaaagctt
    47161 tgctaacttc gatgcaagtg aaaggtggta agtgcatgat catcaatgat aatggcagag
    47221 agtatgatac agaaaagatt gaagagtatt catcttatac tcagggatta attaaacgtt
    47281 tgatatacgt tcgctatgta ggtattaggg atctgttatc agataactgt tgtagtaaat
    47341 acaaagtgaa tcaagtaaga gaagcgttga ataaagataa taacgtcgaa agaataaaaa
    47401 atgtttttgg atatagtatt gaagagatta attattacat tgacttcgct gaagctttca
    47461 ttccgatggt gagataaccc ccccttaaaa taaatcgcaa attttttggg ggtgatgaaa
    47521 cggagggggc tgtcaggaaa agagattttt tcgaacttta tcatgaaagg agggctaaaa
    47581 tgtttaaaaa cgaattgtct caaaatcgct acagagaaaa attacgccgc tctttaataa
    47641 gccaattgga aagtcagaaa acaaatattg agccattctt agataatgtt gatcgttata
    47701 tcagtttatg ggaaacggcg atatcactgg aagaagatat atccgagaac ggcattagac
    47761 tggagaatgg taaaaagaat gaatcagtag cgttgcttgt ttctgtcaac aaacaaatgg
    47821 gattgatgtt ggataaactt gccattactc ctgaattggt aggtgaagca aatgaatcaa
    47881 ttcctgagtt ataagcatat tgaaaattgg ttcaaagcta tagaagaagg cactatcaag
    47941 gtatgcaaag agcaattatt gctaaaaaat tatctagaag aaagagtctt tactagagaa
    48001 gatatttact tcgataagca gatggtagag gattcaatca atataccagc acaatacttt
    48061 ccattcgaat taattccgtg ggaaaaattt ctacaatgtt ttatttatgg tgttcgatgg
    48121 aaaaaagata aaacactagt gttcaataga tatctttcat taatgggacg tggtaatggt
    48181 aaaactggtt ttgcttcttg gaacaacttc tttctactaa ccgctaaaca cggtattaaa
    48241 aattatgata ttgatatcta tgccaataat gaaagccaag caaagactag ttttgatgat
    48301 gtatttaaag taattaaaga tcatcctgat ttagataaaa aagtatttaa agctacgaag
    48361 gaagttattc aaaatatcgc tacaaacagc aaacttcgtt ataacacggc aaatgctaga
    48421 acaaaagatg ggaagcgacc aggtgcaaac cgctttgatg aaattcacga aaatgaagat
    48481 tattcaatga taaatgtggc tacttctggt ggtggtaaaa ttcgagatta tagagaattt
    48541 tatgatacaa ctaatggtca tgttcgtggt ggtccgcttg atgacattat agaagaatca
    48601 aaaatgattc tttctggaga acttggaatt gacaaggatg gagcagaatt ttctagtttg
    48661 tttccattta tttgtcgctt ggataacgat aatgaagttg atgatcccga catgtgggaa
    48721 aaagcttgtc caactattaa ttacaatgca gatttaaaac ggaaaatgtt tcaagaatac
    48781 tctcaaatgc aacgtaatgc tggtttaaga cttacgttca tgaccaaacg aatgaacaga
    48841 cctatggaag atacacgatt tgctgttgct tcatatgatg atgttctgca tacgaaagaa
    48901 aaagaatttc ctgaaaaaat ggatgaagtg ataggaacag tcgattttgc tgatagacga
    48961 gattttgcca gcgttgggtt gctaggaaaa tacgataaag atgtttattt tacacaacat
    49021 acttttatcc acgaatcagc ccttcgatta caaaacatca aacgagaggt tatagatatt
    49081 tctatagatc aaggaaaatc acagatcgtt catggaaaaa atatagaagc tgattatatt
    49141 gtaggttggt ttcttgaaat gagtaataaa tattatatta aaaaaatcgc tatggatatg
    49201 taccgtgcaa aaatattgaa gcccgcttta gaagaagcag gttttactgt ggaaattgtt
    49261 cgaagcggat ctgttacaca tggtatgtta aaagatctgg ttgatgacct ttttattaat
    49321 caacgtttat tttttggtga cgatgcgatt atgcgttggt attgcatgaa tgtatatgaa
    49381 gagcatattt ctaatggaaa tatacgctat gaaaaaatag aacctgaaac tagaaaaacg
    49441 gatggctttt tttcattcct tcatggtttg aattttttag atgatattta tgattctgct
    49501 cctgtaacag tcacaaatag ctcagtagaa aatacaggaa ctggatttac tcctctagta
    49561 ttctaacttg aaaggaggtg agaaagtggg gatttttcaa aaggcggtag gatacttcac
    49621 aaaaaaagca acggttcctt tagaagaata cttttgtaaa ttgcaagttg attttgtgta
    49681 tcgaaaattt gcgattgaaa cttgtattga tttgattgca aatgcgatga gtaaagcgga
    49741 attcaagtca tatgaagatg gaaaaaataa aaagaatgat ctttactata ggctgaatgt
    49801 agctcctaat aagaaaaata atgcaacaga atttagaaaa aaactgatca ggagattaat
    49861 attctacaat gaagtattga tcgtttctcc gtctaataat tctagcgaaa tatttattgc
    49921 ggatagttgg gatgtcacag aatatgcatt gaaagatgat gtgttttctc aagtgcaaat
    49981 taacaacata gtccttgata gagaatttct agaaagtgat gttatctata taaaatacgc
    50041 agatcaacaa attaggcaac tagtcgatgc gtattatcaa gcgtatggga aactcatttc
    50101 tagtgccatg aatgtttaca agcgctctaa cgctcgtaga tacgtactga aagggaattt
    50161 attccgaccg caagacaata caacacaaga tcaaatcaat aaaatgatga catcacaatt
    50221 taaggctttt atggaagctg ataatgcagg tgcggtattt caattacaaa atgagtacac
    50281 attagaagat ttcagcggaa acttccaaag caattcaaga gatataaaaa acttaataga
    50341 cgacatcttt gagatgacag cagcagcgtt tcacgttccg aaaaacctac taaagggaga
    50401 catgagtggg ttatcggatc aagtggacgc ttttttaatg ttcgaaatca taccgattgc
    50461 tgaacttatt caggatgcgt ttaacgctag tctctatgaa gtagaagaat acttgtcagg
    50521 gaattttgta cgtgtggata caactatgat caagattact agcttcaaag atttggttga
    50581 cgctattgat gtaggcatta gaaatggagt atttacaatc aacgaaggaa gagaacgcgt
    50641 tggaaatgat cgctctgata aggcgatggc agatgaaata tttataacta aaaacaatca
    50701 acaagtatcg aaaggaggtg aggcgaatga cgacaatgaa aacatttcta gcagtaaaga
    50761 atgaaggcgc agtaccgcaa atttttattc agggatttat tggttctagt tggttctttg
    50821 aagggaatac tgacaaggga atcaaaaata ttttggatag tctaggtgat caagaagaaa
    50881 ttgaagtagt aattaattca aacggtggag acgtatttca agggattgct attgggaact
    50941 tacttaagtc aaataaagca aaagttaacg ttgtgattaa cggcttagcc gctagtgctg
    51001 cttcaattat cgcaatggct ggcgatacta taaaaatcta caacaatgca caattgatga
    51061 ttcaccgcgc ttccacatac ggagaaggaa atgtcgatga cttccgtacg attgctgacc
    51121 aactggaatc aattgataaa tcggtaaagg cttcatataa aacacgattc aatggcacag
    51181 atgaagcatt gcaagaactt cttgaaaaag aatcgtttat ggatgcagaa acagctttga
    51241 gttatggatt ggtcgatgaa attatcgatg cagaaaatag ctcaggtact gaagctaaaa
    51301 aagaacaaag cgttgaagaa attttgaatg acgttaaaga aaaaagagca gaaaaaattg
    51361 ctgcatttac agcagcatta aataaaacat ttggacaagg agatgtaaaa taatgacagt
    51421 taaaaattta aaaggtgtaa cagctgcaag cgaccaattg atgaaagctt ttaaagatgg
    51481 taacgaagaa tcttttagcg cagctatggt aagcttatct aaggaaattc aggataaaat
    51541 tttagaagaa gcaacagcaa aaaatcaaga tcaattagta ttaatgaacc gtggtcagcg
    51601 tgtattaact acacaagaaa caaaattcta taacgaagta gtgaaaaacg aaggttttgc
    51661 aggggtcgaa gaattagtgc cagctactgt atttgaacgt gtatttgaag atttagaaca
    51721 atctcatcca ctattgcaaa aaattacttt tgttaacaca actggtgtaa cagaatggat
    51781 tgtgtcacgt ggagtcaatc cagcatggtg gggtaaactt tgcgaagctg ttaaaaaagt
    51841 tttagataat ggctttgatg taattaacat gaagcagttc aagctatcag gttatattcc
    51901 tgtatgtaag gcaatgcttg atttaggtcc agtatggtta gatcgttatg tccgtactgt
    51961 tttagtagaa tcattgagaa ttgcattaga acaagcaatt gttgatggta ctggtaaaga
    52021 tatgccagtc ggaatgatgc gtgacatgag caaacaaact agcggagaat atgctgaaaa
    52081 aaaagcagaa cctattacag ctttagatgc tgtaactatg ggcggtttga tggcgcgact
    52141 atcaaaattc aatatcgaag gtgtgaatga tccgatttat cgtaatgtga atccttctga
    52201 tgtggtccta attgtgaatc caactgatta ctggtctaaa gtattcccag ctaagactgt
    52261 actaactgct aatggagaat acgtacaagt attgccagta ccagtttcag atttgcagtc
    52321 aacggctgtg ccagaaggaa aagcagttat tggggtagcc tcagattact tcatgggtgt
    52381 aggatctaca ctaaaaattg aagcttcaga tgaataccat tttgttgaag acgaacgcat
    52441 ttatctagct aaacaatatg caaacggtca acctaaacgt aacgatagtt tcattgtatt
    52501 agatattagc gctttgggaa ctactactac aactacaaaa ccaacaacca caacaactac
    52561 aacacaagcg taggtgatca gaatgaagta tattctttgt cagccggcaa tcaatcggtt
    52621 taaatgggag cttgaagttt gtttaactaa tctgaagaaa ctaggaatca aagatatcgt
    52681 attgcttttc agcagacacg atgatcagat tcctattttt tttgagaagg aatatggtgt
    52741 tgaagttcat gtgtacgatg atctgcggga cgacaaagag tatattcctt cgattaaacc
    52801 atatttatgg tggaaatatt tagaagaaga tcattcgcgt gaggacgacc gatatttcta
    52861 tatcgattcg gatgtcattt tcaataaaag aattaatttg cgcaaattgc cttctaaaga
    52921 tgatgtttgg tattgtagcg actgctgtag ttatctaagt cttgattata ttagaagctg
    52981 tgaaaacgga gaaaatattc taaaagatat ggcaaacatt gtaaatgtta cagtagaatc
    53041 tttggaaact ataaacacta attcaggagg cgcacagtgg gttattaacc gccctaaagc
    53101 gaattattgg aaaaaggttt atctggattc taatcggcta tatcgctacc ttagagggca
    53161 aaaaacaaat atacaaatct ggacagccga gatgtgggca cagctttgga acatgatgta
    53221 tttcaatatt ggtcctaaag ttcacgagga attagacttt tgttttgcta ctgatccaat
    53281 agaaaaagtt aaagaagtaa aaatcttaca caatgctgga gtaacaacaa acgatgaaga
    53341 tttatttttc aaagggagat acgtgacttc tacgcctttt gatgaagatt tatcatttgt
    53401 aaacaagaaa aaatgctctt acgcatatgt taaagcaatt aaggcggtgg ttagatgacg
    53461 cctgaacaag tgactgaaga attgctaata gctgtgaagg ataatattta cgttacctgg
    53521 aacgaagaag atgagtcaat taaaaagatg atagctaaaa atgctgttta tcttcaaagt
    53581 aaagtgagta caacactttc tttttctcct gaaagcttag aatacggatt gctaatcgaa
    53641 agatgtagat acgactggaa tcgtgcttta gatgagtttg aacaaaattt cgctagtgag
    53701 ttattaggtt tcattcaaca ttatgcgcta caagaatata ttgcaggtga tgtgaatggc
    53761 gaataatcgt agactcgaag aaacattcaa cgatggttgg ttaaagattt tgacgcaaac
    53821 taccaaaaga aatgaactag gaaaaaagat tggtgtagaa gatacagaaa tcacttcttt
    53881 aaaatttaga aatctttcca tgagagatag tgatataaca gctatggatg cgatgggatc
    53941 gaaattaact aagaaagtaa agactccatt tcatccaatc gccaagaaat ttaataaaga
    54001 tcaatatttt atcgtaatcg atagtatgcg ttacaacgtt atctatgccg attacgataa
    54061 tttttatatc tatttttatc ttgaaagtgt gggtgaatat ggtgattgat aattctaaag
    54121 aaaaagaacg tttaaataag caaatttctg ctatcaaaac ttccttagaa gagcattttg
    54181 gcctcaaact ctttcaagac tccgtaggcg aggatgagct acctgatgat tttaattact
    54241 tcattctcga aacaggagaa atagaaatga tcactgagcc aaaatatagc gtgggtcaaa
    54301 atctatatct aactttctat tcagaaaata gagaagattt aacaggagat tcactagata
    54361 ttatttcatt gattcaaaat cgttcgattc gttttcagag aatggatccc aatcatttaa
    54421 aactagagaa ccaagatcgc tatatcgatc aattggtatt tacgtttaga cgattattga
    54481 agagtgattg tcatggctaa aaatagttgg gagctaaaaa taaatggaca tgatgaactt
    54541 cttgtgcgga tggaacgcta ttcaagcgag agcgaacgac tgattaacga agcattgaaa
    54601 tcaaaaggtt cggctattgc agtggatagg attacagaaa aaattcctgt ttctgaagca
    54661 gatttaagaa gagggcacca acacgcaaaa aatagtcgtc cacttaagac tcaatacatt
    54721 aatttgggtt tcatcattag acctacaaga aaatttgagt atttaaaata tcctgatttg
    54781 gggataggta cttctaaaag aaatcagccg gacgaattta tgagaagagg attaggtctt
    54841 gcacttgatc caattacaga acttctgatt cgtcaattcg ataaattaaa taaataggag
    54901 gaacaacaat ggctaaaaca acaacagtaa caacttttga caatataagt attaaaagaa
    54961 tttcttttaa ttttaagaat gcaacaaatg caatttcaac tgattgtaat ggacaattag
    55021 atggcgaaac agaaatgcag agaattgtaa aaaaatgcgg ttcaacagaa gtaaaatcga
    55081 aatctaaacc aatcaatatg acggtaacaa ttactgcaca tgtaccgatg gaagtttatc
    55141 gacgcttcaa tgggttgaaa caagatgaac gtattaaatc gggaatttac tcttatggtc
    55201 ctgattccgt aggggaagat ttctctcttg ctgcagagat cgtggatgat ttcgaagaaa
    55261 aaaataagct gattggtatg ttagcatgca cttcgaatac aggattaaca ttctctattg
    55321 aaaatggtgc ggatgaagta gctgcgttag aactagaaac aaaagttatg caagatgaat
    55381 ttggtaaatt ttatcatgaa gcaattgttg cagaacttga agaagactta acagatcaat
    55441 ggatgacgaa tctatctgct gatgtgatta aaaaaacttc agttgtgaca actacggcca
    55501 ctcaatcaca gtaaaaaaaa cggaggtagc gaaatgaacg aagattactc aaaaattgaa
    55561 ctaaacgatg gaacaatttt gaatttagaa cctaaactga atatcaagaa attattgatg
    55621 atcaatagag attttaacac agacgagttt gcaaaaatga ctgtgggaaa aggatccatg
    55681 gatatttctg ttattcaagg tgcaaaggct gtgtatattg cttaccgcca agcgaacatg
    55741 actgattata tttcattcga tgaatttatc gataaatggg attttgatat ggctactgcc
    55801 agctatattt atcaattgat gatgttcaaa caagcacgcg atgcttatca aaaagaattt
    55861 gaaaaagcaa ataaggaaaa aaagcttcaa aagtaaaaat gccaaagctc ttagttgaaa
    55921 cgtgggtcga tgtctattcg atgttgaccg acgttttttc tatgccttca gatttggttt
    55981 taagcgatat ctgtttagat gacattttgc aaatggctta caacaagagt gcttatgaag
    56041 gatggaaaaa ctatgcaata aaccaatccc agaaaaacta aagaaaggag gtaaaaaatg
    56101 gctaaaaaga gaacagaagc agaagtaaca ttcatagcta acgatgacgg attgaaatct
    56161 acgttaaaag aaatcagtgc tgaattaact aaaaatagag cagaattaaa actagaacaa
    56221 gctcaattac aacagactgg ttctgaatca gacaagttag gaagtaaatt atcttcttta
    56281 gaaaagcagt atgaattaca aagtcaaaaa gttgaagtaa ctagccaacg tttagccaat
    56341 gccaaaaaat attatggaga aaattccacc gaagttcaga aacttgagag agaactgatt
    56401 aaccaacaaa cagcgcaaca acgtttgtca aacgaaattg ataaaacgag taatgcacta
    56461 gctcaagcaa aaggcgaaat acagacgtac gagtctacaa tgcaacagtt ggatagtgaa
    56521 caaaaaaatg ttcaagctag tgcttctctg attgaatccg aatacaaaaa atggcaagca
    56581 actgctggtc aatcagcttc tgaatccgag aaattagcga aagcccaaga atatgtttct
    56641 caacaatctg aaaatgcaga gaaaacgata gatatcctga gacgacagtt agaagctaca
    56701 cagtctgagt ttggcgctac atccacagaa gcaatgcaga tggaggcgaa gcttaatgat
    56761 gctgaacgtg aatttgaaga gttaggacaa gctgctaaaa atgtagatac aactaacttg
    56821 gacgatatcg gaagcaaaat agatatgaat aatctaatgg aagcttctga cgttttaagc
    56881 gacattggcg ataagcttac agaattaggg aaacaagcag tggactctgc taatagtgta
    56941 ggtagttccc agagtaaaat acaagctaat tttggtttga ctaaacaaga ggctgaagaa
    57001 ttaacgaatg tagccagaga catttattat aaaggttttg gagaatcgtt agatcagtcc
    57061 acagatgcat tgattttggt aaagcgtaat ttaggcgatt taaataatca agatttacaa
    57121 aatatcacgg aacaagctat ggtcctagaa aacaccatgg gcgctgatat ggatgaaacg
    57181 ttacgtggtg taaatggctt aatggtcaat ttcggcttga gcgctcaaga tgcaatggat
    57241 ttaatggttt cgggtactca aaacggttta gataaaacgc acgaattagg cgacaatatg
    57301 gcagaatata gccaattatg gagtcaaatg ggatattcag ctgatgaaac gttcggaatg
    57361 cttcaaaatg gtttagatgc gggtgcttat aaccttgata aagtcaatga cttagttaag
    57421 gaaatgggaa tatcgttaac agatggtcga tttgagcaaa acatggatat gtttagtgaa
    57481 agtactagaa aagcttttga agagtggaaa aatggcggag gaacacaaaa agacgttatt
    57541 aattccatga ttcaagattt tagcaatatg gatggtcaat acgaccaatt aaataaagct
    57601 tctacaattt ggtctgcact tggcgaagat aacgcgatga aagttgtcca atctttgact
    57661 gatgttaacc atacatttga tgatgttagt ggatctgcac aaaaaatgaa tgaagattct
    57721 actactccgt tacaagaatt aaacggaaaa atagctgaat taaaggattc attagctcct
    57781 ataggcaaca caatcataga tgcactcgaa ccagtaattg attttctagg aaagatggct
    57841 gatgcgttta ataatcttcc acaaccagta caggatttca ttgttgctat aggtggtctt
    57901 acagcagcat ttggcatact gttaccggca atactagccg tgtcatattt atttggtccg
    57961 atgatgctta taattggagg aattatagct gttatagcag gcgttattgt ggcaattaag
    58021 aactggggtg caattactga ctggtttagt ggtttatgga aaaaatttac tgattggttg
    58081 ggtgatactt gggaaagtat aaaagacgga gcctcatcag tttgggatgg ggttaaagaa
    58141 acctggtctg gatttgtaga ttgggttcaa gatatttggc aaggagtttc tgattggttt
    58201 ggagagctat ggagcggatt agttgaagga gcttccaaca tctggcaagg agtccaagaa
    58261 acttggcaaa cattcgttga ttgggtttca aatatttgga acggagtcaa agaagtatgg
    58321 tcgattattt gggcagacat tgtaggaatt gttcaaatac catggacatt aataacgtca
    58381 ttgattcaag ctggtattaa tattatcgtg ggtatttttg atgtagctgg acagttattg
    58441 ggcgcagctt ggcaagctgt ttggacacct atttctgatt tccttaaaaa tacttgggat
    58501 actatgacac aatggataag tatcgcttgg aatgggattg taactacatt ccatactata
    58561 tttgatccag tagtggcatg gtggaatggt atatggacag ctattagtac tacggcttca
    58621 aatatttgga attcaattag tgcaacagct tctagtattt ggaacagtat caagaataca
    58681 atcactagct tggtacaagc agctgctaca gtaattcaaa atatttggtc aactgtatct
    58741 agttggttag gtggaatttg gaattcaatc agctctacag catcaaatat ctggaatagc
    58801 gtgactagta gtataagcaa tgctataaac gcagctaaaa gtgccattca aagtgtttgg
    58861 aatagtatat cttcgtggat tagcggaatt tggaacggta tcaaaaatac tgctttgaat
    58921 ctttggaatg gaattacaag cactattagc tctaaagtaa acgatggaaa aaatgcaatt
    58981 tcaagcggtt ggtccaatct aacaggtatt gtttccgaca tattcaataa tgttaaaagt
    59041 acaattgcta acatatggga aggcatcaaa aagactgtta gcgctccaat tgattggatt
    59101 agagacaaaa tcagtggcat ttttgataat ttgaatattt ctataccaca tattccgtta
    59161 ccggaattta tcatggaagg cagctttaac ccgctaaaag gtcaaatccc ccatttgcgt
    59221 gtaaaatggc atgctaaagg aggtatcttt actaaaccaa ctttactagg tggaatgaac
    59281 ggtgtcggtg aagcaggacc tgaagcagtt ttacctttga aaagatctgt tttgcaagaa
    59341 attggtgatc gtatcttgag tagcacatca gtttcatcta gggcacaaac gattcaacct
    59401 gtgaataact acgaattcaa tttcacaatt gatggtaacg cagatgaggt tactatgaag
    59461 caaacaactc aacaaatcat tgatagcatt acaaaaattc aaaatgataa tgcttcggca
    59521 tggcgttaaa caggagagta tttctcctgt ttttttagta ttaaaaagga tgtgaaaaaa
    59581 tgactgattg tatacattct ataatcgatg gatttcctga ttatttgcat aaattggctt
    59641 tagctgaaag accaaccata ccttctccaa aaagacagag agttgaaact tctgttttag
    59701 gtaggttagg tggcttagta caagattact cgtttgaaga tatgtcgttt acattgcact
    59761 ataactattt agaggatgtg gaagaccatc aagcgttcaa gcaatcgttt tatatcatgc
    59821 gtcattggtt aaactatgca aagaaattag aattctctga cgatcccaac gtctattacg
    59881 ttatccagac tatcgatatt ggggatgcag aaaacgatat tgttgaatgg ggagagttcg
    59941 atgtaaatat tactgcgaaa ccattcgcaa gagttcaaga agatgtacct ataaccgtag
    60001 ataaaccaca gtcatttaac ttgctgaata atagtttaga agaaagtttt ccaaagatta
    60061 tcatcactcc ttcagctact tcatgccagt tcatcttaaa tgattatgtg tttagtttcg
    60121 aaggcttagt agcaggaact gatgtagtca ttgatagtga tttgatgctt tgctacgaag
    60181 agcaatcgga cggagatatt ttagatcggt ccaacaaaat gaagaccatg caatatccga
    60241 cattgcaagt ggatatcaat cattttaatt gcactggttt gagcaaaata caaatttatc
    60301 gtaacgggtt aaggtaggtg aaatagatga tcgataattt aataactatt tacgataaaa
    60361 atgacgcgaa taatttagct gaacatttat atgatacgca aggtttaggc gctttgtcag
    60421 actggttaac agctactgtt agcaataaac taaacggagc cgagatattt cagggtactt
    60481 attcaataaa cggaactaat gcagatttga ttgtagacgg acgtattatt cagtgttatg
    60541 tagatgaaaa tcgagcaaaa cagcgtctac ggatttatta tgcaaaaact tctgtaatag
    60601 gcaatacgat agaagtaaaa gctgaaccta ttttcaatga tataagaaaa tcggtgttga
    60661 ataaatatga cagcggaaca gaaaagatca ctgctactca ggcatggcaa aacgcaaaag
    60721 ttttagcgaa accagctatc ccttcgcagt tttctttctc gtcattagta gatacgcttg
    60781 ctaatgtgaa gatagaaaag gcgaattttt tagaattctt tggtggaaaa gagggatcta
    60841 ttctagatcg atttcatggt gagtttctaa aagataataa cacattacgt catgaaaaaa
    60901 gtctaggtac ggatcataaa atcaaagcga tttatactaa aaacttaact ggtcttgact
    60961 tagagattga tgctcaaagt gttttagttg gagtttatcc attcattagc agctcttcag
    61021 aaggagaaga cgagatcact ctaccagaag aagttatttt cacggattac gtggatgatt
    61081 atcctgctgg atatgtttct tttgttgatt ttaaagacaa agcgactgat gtagccacat
    61141 taagggaagc tgctaaagac tggttgaaaa caaacataga taaacaaaaa ccacaagtga
    61201 gtggttcgat tgaattagta ccattgaggc atcaaagagg ctatgaaaaa tttgttgatc
    61261 tagaaaaagt ttcgatgggt gacggagtag atgtgtatca tccacagtta aaagtgaata
    61321 tgtcagcgag aattgtggaa tatacgttta atgttctaac caattcatac gataaattag
    61381 ttgtaggaaa cgtcaaaaca aacttcttag aaaacacaga gaataatgtc agcaatttga
    61441 ttaatgatgc cattgatcaa ttgaaaaacg gtggcgaaat cagtgattta ctcaatgata
    61501 ttgtagatca tcaaactgat atgattactg gtcaaaatgg tggttatgtt ttattagatc
    61561 ctaaagaagc gcctagtcgt attttgatta tggacacacc agataagaat accgcaagga
    61621 atgttttaca aatcaacaat gctggtattg gtttttctaa aactggcatt aatggaacat
    61681 atgaaacggc atggacgtta gatggcggat tcaatgcctc gtttattacg gctggtgaaa
    61741 tagtaggaat tactattaga ggtactacat taattagtga tggtgctgat tatagaacaa
    61801 gtattgctaa tggcaaaatg acttggtact caaaaaaagt taacaaagat attatggagc
    61861 tagaagcacg tgattatgta agtgctgatg ccggtattgt atcatacacc atgaaaactg
    61921 gtggtggttt catgattaga aatccacagg gtaacttggt ttttagtacg tgggataatg
    61981 gtaataacag accgtttcta tcttttggtg cgcccaattt caggtatagc aatgctagtt
    62041 atgtaacttc tggcgacggt agttctttaa gcattaatgg tagtgcgggt aactcatggg
    62101 aatttaaggt agctggtagg actatgaaat ttactagtga tggtatgcta acgttaccag
    62161 gttgtttttt tggttcatgg gaagatggga aacttgctag gtttgaacaa tcaacggtac
    62221 aagtatataa agattttact gttagaggta ctaaaaactc aactgtacca acagaacatt
    62281 atggacaacg actattgaac gcttatgaaa ctccagaata ttatttcgct gattatgggg
    62341 aagccgttac aggtgacaat ggtaaagttc gtgttgatat tgaccccatg tttgctgaaa
    62401 cagtaaatct aagtcggtat atgacacatg tgacacctac agaactagtt ttgtgtgctg
    62461 ttactcatga agatattgac catttcatca ttgaaactag taagccaaac gtattagtta
    62521 gatggaattt agtggcacac cgtctagggt atgaagatat tagattaaaa gaggatacag
    62581 catatgatag cacagtgctt gaccaaaaac gtttttaaaa cgaagacaag gaggtatata
    62641 aatggctagc agtttatata atttggcttt agatttcagc aaagaattaa actacaccaa
    62701 agctattatg gctcgtcaag gtgataaagg gattacggtg acggttaaac cgtttctaaa
    62761 tggcttgcag atggatacga gtggcggaac atttacttta aaaggaacaa caccatctaa
    62821 ccgttacgta gataatgttg caactagtgt aactagtgaa gaagtcacgt tttctcttga
    62881 tggcacattt atgagtgaag caggatatta taaacactgc tacgtagaat atagaaaaga
    62941 caatcaaatt ttaacaacgc aagatatcat ttttttctca ctaggagtgt ctgacatttc
    63001 gcaaggccaa gccgatgaat atgtttcgca attagaagag ttgattcgaa agtacaacga
    63061 aacttttgat gcttttatgg ctgaaatcaa aggtagagtg gatagcttaa atcaacagat
    63121 tactgattta actggtcaag ctaaaacgct acaagacaag ttagatgctc tgaaagaaga
    63181 aatttctaag ttaggtaact tacaagtgat gtacagtaac agcatcgact tcggggacta
    63241 tgattattcg gagaacccga atttaatgcc atatattaca gaaccttggg ttggaccttt
    63301 gttaggtaat ggacatacag ttaaagatag tgttaaaagg gttataactc atactaaaac
    63361 tagaacagct aattccggcg acatattatc gcttggctta ggtattccgt gtacagcgga
    63421 agctaataat aggtatctta taaccacttt acgaccatct acgacataca cattaagcgt
    63481 tactatgagt gtcggttcag attggactgg agagaccaat actatagggg ttaggcttag
    63541 atatttaaat gaacaaggtg gtatagaact gcccattaat gcattaatac cagcaaatgt
    63601 agaacgcgat aaaatggtca ctcatacatt tactggtata acaaaagata atgtaactag
    63661 cataacaaac tgttatgttg aaatattctc acttaatagt gaatataaag gaacggttag
    63721 tgtcagctat gacgttaagt taaaagcaca ctaccctaac ttactagatg gcccttactg
    63781 gttaggtaaa gttcctttag gcgagaatat tgctgaccct acagttgtct ttccacacaa
    63841 aactagtgaa tatatggttt atggtagacg caatacagaa aactatatcg ctgaccaaac
    63901 atataccata tcaatgaaag caactaaact aacagttcaa tcgtttgccg tgtatattgc
    63961 tgctggccgc gttaaagtag gagacatgaa gcctactgag gggttagcta atacatggga
    64021 gttaactttt actgtaacta agcaacatat agatagtggg gtaactaatt atctggaaat
    64081 ataccaatac ccgtcagcta caaaaggagc tgttcaaatt gagtggctca aactagaaaa
    64141 aggcaatact cgaaccccga atattagtga gtataaatat cgtggtactg gtatgcgtga
    64201 ttcaaacaat ccaaaagatt atgtttggga tctagcacca gaatatgtcg aagataattt
    64261 ggccacagat attaaaattt ctgaaattac tggtaaagca aacaattata ccgatgggaa
    64321 agtatcggag attaattcgc agttgactgc ttcaattaat gaagtagaca ccacagctaa
    64381 ggatgctcaa acaaaagcga atgctaatgc gactgctata gatgaattag acaataagat
    64441 cgatgaacgc attaatgata cagctactac cacattaaca gttacaaacg ggaataccgg
    64501 atcagcaaag ctttatcgtg aaggaaaaac agtttctata tattttgtgg ctttaaacgg
    64561 aaaacgcagt ggtggaaatg attcaacgat actaacaatt ccagaaggct atcggccacc
    64621 aattagtttt gagcaactgg ttggctcgat agaccgttct actttgaaca gtgctcagtt
    64681 atctattggt gcagatggag ccattaaatg gcgaagaaac tcaagttatg gatcggatta
    64741 tacctttgca attacttaca cgatttagaa aagcgtgaat cgatatgaag gcagcatata
    64801 gaccaattga accttacgga ttcgagcaaa tcattgtgaa tgatgaagaa catttaccgg
    64861 aagaatgcac agaagtcgaa ccaccgattc caaattggaa accgaaattc aattactggg
    64921 agggaaataa atgaaaaaca tttggaaata tggacgtact ggcggagagt acgcaggaaa
    64981 agtattggac gacatgcttg tatccgttcc ttacacagat cagccaccgc ttgaaggaat
    65041 tcgtgctgat ggcgaaccgt taacaatcgc tgatcaaatg tttgatccta aattgaatca
    65101 atggattatt ttagcgaacg cactagatca caacgattta aacaatctca aagcaatgta
    65161 tgagtcgtta gaaaatgaga acggcgattt aaaacagatc aatgccaaac tcatgctaag
    65221 cgatgtagca attaaacagg aaaatactgc attgaaagaa aaagcggata gtttagcaca
    65281 aatcaattca aaaatgatgc ttgcttcgtt acaaaatagc aaagacattt cagaaattaa
    65341 agagcaacta aatccagctt caaagggagg tgagtagtat gtttagtttt agcgatgtga
    65401 aaatgatgta tgattggggc tgtttcacta acgaacaagt aatggttttc gttccgttgt
    65461 gcattactga agaaaaagca gataaaatca ttagcaaaga agagagcgca tcttaattga
    65521 tgtgcttttt attttgattc aaggagttgt cacatgatta atttagggga atggggagcg
    65581 atagcaggat caataaccgc tatcgtttct ttgattttat tagtaataaa accaattact
    65641 gcatctttct cgaagattac tgagactctt tcaaaagtaa gtcacaattt agatttgctg
    65701 actaaagatt tagaatcgag caaatcagat cgattgatga ttcatgaaga actaaagaaa
    65761 cacgatgaaa gattagatac acatgcagaa aaattggtag aacacacgca acaaattaaa
    65821 actttattta gggagagaag aaaatgaata ataaaacgtt cgaagtacta aaatggttcg
    65881 cactggtaat tattcccgca ctagctactt tcgtggggtt agttggtaaa gcgctcaatt
    65941 ggcagtacac agatatctgt gttgtcatca ttactggttt tggcgcgttt ttagggagtg
    66001 tgttgggtgt atcaaatcga acctacaaaa tgttctcggc tgaaagcgaa gaaggaggaa
    66061 acaaatgaaa aagaaaatta ctattactgc gatgagcctg ttaacggctc tttttttatt
    66121 acccattaat acgtttgctt atactattaa tgacgagtat aatttagcgc cgaatcaagg
    66181 agactccaga ttagcaattc ctaacaaaat tattttgcac gaaactggaa tagatgcacc
    66241 agcaagaaac gtagccgcca acatgaaaaa taattataac ggaagcaatt cttatactac
    66301 agatgttatt ggtgacggtg ggattgttta ccgtgtgggt gagcaaggat atgtttcgtg
    66361 gggagctggt aacgctaatc cttatgcgcc tgtacagatt gaattacagc gcacatatga
    66421 taaagcattg tttgaaaaaa actatcgagc ttatattgaa tatacaagag atagtgcaaa
    66481 aaaatatgga attccattga ctcttgatca aggaacttct ttatttacaa aaggaatcat
    66541 ttctcatttg tgggtgacaa attatgtttg ggggaaccac acagatccat atggttactt
    66601 atcgcaaatg ggagttagca aagaaaagct tgcttatgat ttagctcatg gatttaccga
    66661 tgaaaatcca acaacttctg aaaacaagcc tgtcattgat ccaacacgag ctggtgcagc
    66721 taatcctacg ctgacagatg gaacaaatca cgcccacatt gatcagtttg gggaaatcga
    66781 aaacgcaaac ttgcatgtgg ctggatggca cattgctaac tataaatacg agtatatttt
    66841 cattatggac tacaatactg ggaaagaatt agctcgagta agagctgatg gaatttatag
    66901 accagatgta aaccaagctt ataatacttc tggaaatgtt ggttatcatg tatctttcaa
    66961 tatgcgtaat tttcctagta agaaagtcta tgtaatgatg cgggcaacga atgatccaga
    67021 gggaaacact aaaggcggtg cgcaagattt ccatgacaaa cgttggtatt taaatattcc
    67081 taaacgataa aaatagctcc tcgttgagga gcagtacata actatattga caactataaa
    67141 aattattcga taaaatagtg atgttatcgc atatcttcac tatcacccat aaatagtcac
    67201 actccaagct atgcgataac aggtttgttg ccacacattc tactggttga tt
//
LOCUS       DACDYI010000004        61939 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.004, whole genome shotgun sequence.
ACCESSION   DACDYI010000004 DACDYI010000000
VERSION     DACDYI010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.004"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(79..765)
                     /gene="gpmA"
                     /locus_tag="IWM52_000233"
     CDS             complement(79..765)
                     /gene="gpmA"
                     /locus_tag="IWM52_000233"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP9972519.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(937..1518)
                     /locus_tag="IWM52_000234"
     CDS             complement(937..1518)
                     /locus_tag="IWM52_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9972520.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="IWM52_000235"
     CDS             complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="IWM52_000235"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAP9972521.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2653..3066
                     /gene="rpsL"
                     /locus_tag="IWM52_000236"
     CDS             2653..3066
                     /gene="rpsL"
                     /locus_tag="IWM52_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAP9972522.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3150..3620
                     /gene="rpsG"
                     /locus_tag="IWM52_000237"
     CDS             3150..3620
                     /gene="rpsG"
                     /locus_tag="IWM52_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAP9972523.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3714..5801
                     /gene="fusA"
                     /locus_tag="IWM52_000238"
     CDS             3714..5801
                     /gene="fusA"
                     /locus_tag="IWM52_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP9972524.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5973..7160
                     /gene="tuf"
                     /locus_tag="IWM52_000239"
     CDS             5973..7160
                     /gene="tuf"
                     /locus_tag="IWM52_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP9972525.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7567..7875
                     /gene="rpsJ"
                     /locus_tag="IWM52_000240"
     CDS             7567..7875
                     /gene="rpsJ"
                     /locus_tag="IWM52_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAP9972526.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7906..8535
                     /gene="rplC"
                     /locus_tag="IWM52_000241"
     CDS             7906..8535
                     /gene="rplC"
                     /locus_tag="IWM52_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAP9972527.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8563..9186
                     /gene="rplD"
                     /locus_tag="IWM52_000242"
     CDS             8563..9186
                     /gene="rplD"
                     /locus_tag="IWM52_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAP9972528.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9186..9476
                     /gene="rplW"
                     /locus_tag="IWM52_000243"
     CDS             9186..9476
                     /gene="rplW"
                     /locus_tag="IWM52_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAP9972529.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9515..10348
                     /gene="rplB"
                     /locus_tag="IWM52_000244"
     CDS             9515..10348
                     /gene="rplB"
                     /locus_tag="IWM52_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAP9972530.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10391..10669
                     /gene="rpsS"
                     /locus_tag="IWM52_000245"
     CDS             10391..10669
                     /gene="rpsS"
                     /locus_tag="IWM52_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAP9972531.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10692..11039
                     /gene="rplV"
                     /locus_tag="IWM52_000246"
     CDS             10692..11039
                     /gene="rplV"
                     /locus_tag="IWM52_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAP9972532.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            11053..11709
                     /gene="rpsC"
                     /locus_tag="IWM52_000247"
     CDS             11053..11709
                     /gene="rpsC"
                     /locus_tag="IWM52_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAP9972533.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11712..12146
                     /gene="rplP"
                     /locus_tag="IWM52_000248"
     CDS             11712..12146
                     /gene="rplP"
                     /locus_tag="IWM52_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAP9972534.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12136..12324
                     /gene="rpmC"
                     /locus_tag="IWM52_000249"
     CDS             12136..12324
                     /gene="rpmC"
                     /locus_tag="IWM52_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAP9972535.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12349..12615
                     /gene="rpsQ"
                     /locus_tag="IWM52_000250"
     CDS             12349..12615
                     /gene="rpsQ"
                     /locus_tag="IWM52_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAP9972536.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12673..13041
                     /gene="rplN"
                     /locus_tag="IWM52_000251"
     CDS             12673..13041
                     /gene="rplN"
                     /locus_tag="IWM52_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAP9972537.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13078..13386
                     /gene="rplX"
                     /locus_tag="IWM52_000252"
     CDS             13078..13386
                     /gene="rplX"
                     /locus_tag="IWM52_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAP9972538.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13414..13953
                     /gene="rplE"
                     /locus_tag="IWM52_000253"
     CDS             13414..13953
                     /gene="rplE"
                     /locus_tag="IWM52_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAP9972539.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13972..14157
                     /locus_tag="IWM52_000254"
     CDS             13972..14157
                     /locus_tag="IWM52_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAP9972540.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14193..14591
                     /gene="rpsH"
                     /locus_tag="IWM52_000255"
     CDS             14193..14591
                     /gene="rpsH"
                     /locus_tag="IWM52_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAP9972541.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14623..15159
                     /gene="rplF"
                     /locus_tag="IWM52_000256"
     CDS             14623..15159
                     /gene="rplF"
                     /locus_tag="IWM52_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAP9972542.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15320..15676
                     /gene="rplR"
                     /locus_tag="IWM52_000257"
     CDS             15320..15676
                     /gene="rplR"
                     /locus_tag="IWM52_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAP9972543.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15697..16197
                     /gene="rpsE"
                     /locus_tag="IWM52_000258"
     CDS             15697..16197
                     /gene="rpsE"
                     /locus_tag="IWM52_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAP9972544.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16212..16391
                     /gene="rpmD"
                     /locus_tag="IWM52_000259"
     CDS             16212..16391
                     /gene="rpmD"
                     /locus_tag="IWM52_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAP9972545.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16435..16875
                     /gene="rplO"
                     /locus_tag="IWM52_000260"
     CDS             16435..16875
                     /gene="rplO"
                     /locus_tag="IWM52_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAP9972546.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16875..18170
                     /gene="secY"
                     /locus_tag="IWM52_000261"
     CDS             16875..18170
                     /gene="secY"
                     /locus_tag="IWM52_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAP9972547.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18232..18879
                     /locus_tag="IWM52_000262"
     CDS             18232..18879
                     /locus_tag="IWM52_000262"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAP9972548.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19070..19288
                     /gene="infA"
                     /locus_tag="IWM52_000263"
     CDS             19070..19288
                     /gene="infA"
                     /locus_tag="IWM52_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAP9972549.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19321..19437
                     /gene="rpmJ"
                     /locus_tag="IWM52_000264"
     CDS             19321..19437
                     /gene="rpmJ"
                     /locus_tag="IWM52_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP9972550.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19455..19820
                     /gene="rpsM"
                     /locus_tag="IWM52_000265"
     CDS             19455..19820
                     /gene="rpsM"
                     /locus_tag="IWM52_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAP9972551.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19848..20237
                     /gene="rpsK"
                     /locus_tag="IWM52_000266"
     CDS             19848..20237
                     /gene="rpsK"
                     /locus_tag="IWM52_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAP9972552.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20317..21255
                     /locus_tag="IWM52_000267"
     CDS             20317..21255
                     /locus_tag="IWM52_000267"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAP9972553.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21283..21663
                     /gene="rplQ"
                     /locus_tag="IWM52_000268"
     CDS             21283..21663
                     /gene="rplQ"
                     /locus_tag="IWM52_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAP9972554.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21935..22774
                     /locus_tag="IWM52_000269"
     CDS             21935..22774
                     /locus_tag="IWM52_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9972555.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22750..23619
                     /locus_tag="IWM52_000270"
     CDS             22750..23619
                     /locus_tag="IWM52_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9972556.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23609..24406
                     /locus_tag="IWM52_000271"
     CDS             23609..24406
                     /locus_tag="IWM52_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP9972557.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            24609..25355
                     /gene="truA"
                     /locus_tag="IWM52_000272"
     CDS             24609..25355
                     /gene="truA"
                     /locus_tag="IWM52_000272"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP9972558.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(25810..28479)
                     /locus_tag="IWM52_000273"
     CDS             complement(25810..28479)
                     /locus_tag="IWM52_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP9972559.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(28717..28905)
                     /locus_tag="IWM52_000274"
     CDS             complement(28717..28905)
                     /locus_tag="IWM52_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAP9972560.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            29175..29537
                     /locus_tag="IWM52_000275"
     CDS             29175..29537
                     /locus_tag="IWM52_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9972561.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            29589..31271
                     /locus_tag="IWM52_000276"
     CDS             29589..31271
                     /locus_tag="IWM52_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAP9972562.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            31388..33424
                     /gene="recG"
                     /locus_tag="IWM52_000277"
     CDS             31388..33424
                     /gene="recG"
                     /locus_tag="IWM52_000277"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAP9972563.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            33473..34474
                     /gene="plsX"
                     /locus_tag="IWM52_000278"
     CDS             33473..34474
                     /gene="plsX"
                     /locus_tag="IWM52_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAP9972564.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            34596..34838
                     /gene="acpP"
                     /locus_tag="IWM52_000279"
     CDS             34596..34838
                     /gene="acpP"
                     /locus_tag="IWM52_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP9972565.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            35106..36110
                     /locus_tag="IWM52_000280"
     CDS             35106..36110
                     /locus_tag="IWM52_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9972566.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            36111..37055
                     /locus_tag="IWM52_000281"
     CDS             36111..37055
                     /locus_tag="IWM52_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9972567.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            37055..38017
                     /locus_tag="IWM52_000282"
     CDS             37055..38017
                     /locus_tag="IWM52_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9972568.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            38044..38958
                     /locus_tag="IWM52_000283"
     CDS             38044..38958
                     /locus_tag="IWM52_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9972569.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            38984..40765
                     /locus_tag="IWM52_000284"
     CDS             38984..40765
                     /locus_tag="IWM52_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9972570.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            41113..41799
                     /locus_tag="IWM52_000285"
     CDS             41113..41799
                     /locus_tag="IWM52_000285"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP9972571.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            41819..45400
                     /gene="smc"
                     /locus_tag="IWM52_000286"
     CDS             41819..45400
                     /gene="smc"
                     /locus_tag="IWM52_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAP9972572.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            45409..46218
                     /locus_tag="IWM52_000287"
     CDS             45409..46218
                     /locus_tag="IWM52_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9972573.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            46229..47227
                     /gene="ftsY"
                     /locus_tag="IWM52_000288"
     CDS             46229..47227
                     /gene="ftsY"
                     /locus_tag="IWM52_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAP9972574.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            47414..49681
                     /gene="gshAB"
                     /locus_tag="IWM52_000289"
     CDS             47414..49681
                     /gene="gshAB"
                     /locus_tag="IWM52_000289"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAP9972575.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKDR
                     RLTMNVLKLLYPEVF"
     gene            complement(49770..50222)
                     /locus_tag="IWM52_000290"
     CDS             complement(49770..50222)
                     /locus_tag="IWM52_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAP9972576.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            50372..51322
                     /locus_tag="IWM52_000291"
     CDS             50372..51322
                     /locus_tag="IWM52_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP9972577.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            51315..52274
                     /locus_tag="IWM52_000292"
     CDS             51315..52274
                     /locus_tag="IWM52_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP9972578.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            52271..53026
                     /locus_tag="IWM52_000293"
     CDS             52271..53026
                     /locus_tag="IWM52_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9972579.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            53049..54002
                     /locus_tag="IWM52_000294"
     CDS             53049..54002
                     /locus_tag="IWM52_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9972580.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(54646..54942)
                     /locus_tag="IWM52_000295"
     CDS             complement(54646..54942)
                     /locus_tag="IWM52_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAP9972581.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            55297..55653
                     /locus_tag="IWM52_000296"
     CDS             55297..55653
                     /locus_tag="IWM52_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9972582.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            55663..56562
                     /locus_tag="IWM52_000297"
     CDS             55663..56562
                     /locus_tag="IWM52_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAP9972583.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            57079..57810
                     /locus_tag="IWM52_000298"
     CDS             57079..57810
                     /locus_tag="IWM52_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP9972584.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            57955..59982
                     /locus_tag="IWM52_000299"
     CDS             57955..59982
                     /locus_tag="IWM52_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAP9972585.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            60090..61187
                     /locus_tag="IWM52_000300"
     CDS             60090..61187
                     /locus_tag="IWM52_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP9972586.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            61494..>61939
                     /locus_tag="IWM52_000301"
     CDS             61494..>61939
                     /locus_tag="IWM52_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9972587.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYVSVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT        20459 a        11084 c        13212 g        17184 t
ORIGIN      
        1 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
       61 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
      121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
      181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
      241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
      301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
      361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
      421 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
      481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
      541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
      601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
      661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
      721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
      781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
      841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
      901 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
      961 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
     1021 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
     1081 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
     1141 taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa
     1201 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
     1261 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
     1321 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
     1381 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
     1441 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
     1501 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
     1561 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
     1621 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
     1681 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
     1741 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
     1801 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc
     1861 tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc
     1921 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
     1981 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
     2041 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
     2101 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
     2161 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
     2221 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
     2281 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
     2341 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
     2401 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg
     2461 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
     2521 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
     2581 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
     2641 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
     2701 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
     2761 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
     2821 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
     2881 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
     2941 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
     3001 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
     3061 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
     3121 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
     3181 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
     3241 tggttgacgg aaaacgcggg attgcagcta acattatcta taatgccttt gacttgatca
     3301 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
     3361 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
     3421 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
     3481 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca
     3541 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
     3601 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
     3661 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
     3721 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
     3781 aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa
     3841 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
     3901 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
     3961 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
     4021 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
     4081 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
     4141 acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc
     4201 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
     4261 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
     4321 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
     4381 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
     4441 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
     4501 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
     4561 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
     4621 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
     4681 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
     4741 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
     4801 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
     4861 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
     4921 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
     4981 gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg
     5041 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
     5101 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
     5161 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
     5221 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
     5281 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
     5341 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
     5401 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
     5461 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
     5521 tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa
     5581 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
     5641 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
     5701 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
     5761 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttgaa accggatagt
     5821 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
     5881 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
     5941 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
     6001 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
     6061 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
     6121 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
     6181 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
     6241 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
     6301 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
     6361 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
     6421 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
     6481 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
     6541 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
     6601 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
     6661 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
     6721 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
     6781 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
     6841 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
     6901 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
     6961 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
     7021 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
     7081 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
     7141 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
     7201 taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga
     7261 tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt
     7321 ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga
     7381 gtttatatat attataatga caaaggtgcg tttactttag tgaattgcac agacgtaatc
     7441 agaatgattt cgtcggctaa gaaacgagag gttgcgacac gcccggacgc tttgccacga
     7501 acgagcgtga cggaaatttt cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg
     7561 aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta
     7621 gatcaatcag cggataaaat cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt
     7681 ccaattccat taccaactga acgcagtctt tacacaatca tccgtgcgac tcataaatac
     7741 aaagattcac gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga cattgtgaac
     7801 ccaacaccaa aaactgttga tgctttgatg aagctagact taccatctgg tgtaaacatc
     7861 gaaatcaaat tataaaacta aaacaaaaat aatggaggtg tactcatgac caaaggaatc
     7921 ttagggaaaa aagtgggaat gacacaaatc ttcactgaat ctggtgaatt aattccagta
     7981 actgttgtag aagctactcc aaacgtagta ctacaagtga aaacaatgga aacggatggc
     8041 tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg
     8101 aaaggtcatg ttgcaaaagc aaacacggct cctaagcgct tcattcgtga attcaaaaat
     8161 gttgagctag gagaatatga agtaggaaaa gaaattacag tagatgtttt ccaagcagga
     8221 gacattattg atgtaacagg tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc
     8281 cacggacaaa gccgcggacc aatggctcac ggttctcgtt accatcgtcg tcctgggtca
     8341 atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt
     8401 ggcaaccgcg taactatcca aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt
     8461 atcttgatca aaggtaatgt tcctggagct aaaaaatctt taattactat caaatcagct
     8521 gtgaaagcta aataattgta ggaagaaagg aggaactaag gaatgccgaa tgtagcatta
     8581 ttcaaacaag atggaagcca aaacggtgaa atcacactta acgaagaaat cttcggaatt
     8641 gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa
     8701 ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt
     8761 caaaaaggaa caggtcgtgc acgtcaagga tctatccgct caccacaatg gcgtggaggt
     8821 ggcgtagtct ttggaccaac accacgttct tacagctaca aacttcctaa aaaagttcgt
     8881 cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg aaaacaactt ggtagcaatc
     8941 gaaggcttga acttcgacgc accaaaaaca aaagaattca aacaagttct tgctaactta
     9001 tctatcgact ctaaagtatt ggttgttcta gaaaaaggta acgactttgc tgctttatca
     9061 gcacgtaact taccaaacgt ttctgtagta acttctgata atgtgagtgt gctagacgtt
     9121 gtatcaaata caaaagtgtt agcaacacaa actgctctta ctcaaattga ggaggtgctt
     9181 gcataatgaa cttactagac gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg
     9241 ccatggatga caagaaatac acttttgaag tggacactcg cgcaaacaaa actttagtaa
     9301 aacaagcggt agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac
     9361 gtgcgaaatt caaacgtatg ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga
     9421 tcgttacatt gactgaagat tcaaaagaaa tccaattatt cgaagctgct gaataagcac
     9481 taatagataa ctcattaaat taggagggaa aaacgtggcg attaaaaagt acaaacctac
     9541 ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa catcaacacc
     9601 tgaaaaatca ttgttacaac cattgaaaaa ccatgctgga cgtaacaaca acggccgcat
     9661 cactgtacgt catcaaggtg gcggacacaa acgccaatat cgcgtgatcg acttcaaacg
     9721 taacaaagat aacgttgttg caactgttca aacgatcgaa tatgatccaa accgttcagc
     9781 taacatcgcg ttagtacact atgaagatgg agtgaaagct tacatcttag cacctaaagg
     9841 actagaagtg ggtatgaaac ttgtttcagg tccagaagca gatatcaaag tcggaaatgc
     9901 tcttccatta gaaaacattc cagttggtac tgttatccac aacattgaaa tgaaacctgg
     9961 taaaggtgga caattgatcc gttctgctgg tacaagtgct caagtacttg gtaaagaagg
    10021 caaatacgta ttgatccgct tgaactctgg tgaagttcgt atgatcttag ctgcatgccg
    10081 tgctacaatc ggttctgtag gtaacgaaca acacgaatta atcaacatcg gtaaagctgg
    10141 acgctctcgt tggatgcgta aacgcccaac tgtacgtggt agcgtaatga accctaatga
    10201 tcacccacac ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc
    10261 ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat cagacaaact
    10321 tattgttcgt cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac attttgagag
    10381 gaggttcacc atgggtcgta gtttaaagaa aggacctttt gtcgatgagc atttaatgaa
    10441 aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg
    10501 ttcaacagtt ttcccaagtt ttgttggata taccatcgca gtttatgatg gacgtaaaca
    10561 tgtaccagtt tacatccaag aagacatggt aggacacaaa ttaggtgaat ttgcacctac
    10621 aagaacttat cgtggacatg ctgctgacga taagaaaacg aaacgctaat ttgaggggag
    10681 gataaatcca aatggcagaa caaatcacat cagctaaagc aactgcaaaa acagttcgca
    10741 cttcacctcg aaaagcccgt ttagtaattg accttatcag aggtaaaagt gtcgcagatg
    10801 caatttcaat cttgaaattc acaccaaaca aatctgctgg aatcattgaa aaagtattga
    10861 tgtcagcagt tgcgaatgca gaaaataact ttgacttaga tgttgaaaac ttggtagtaa
    10921 gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat
    10981 cagcttctcc aatcaacaaa cgtacaagtc atatcacggt agtcgtatca gaaaaataag
    11041 gagggataat cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg catcatccgc
    11101 gactgggatg ctaaatggta tgctgaaaaa gagtatgctg agttcttaca cgaagattta
    11161 agaatccgta aatttatttc aactaaactt gctgatgctg ctgtatcaac agttgaaatc
    11221 gaacgcgcaa caggtcgcgt aaacatttca attcacacag ctaaaccagg tatggttatt
    11281 ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat taaacaaatt aactggtaaa
    11341 agaatccata tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt
    11401 gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc
    11461 atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta
    11521 aacggagcag atatcgctcg ttcagaagga tactcagaag gaactgttcc tcttcatact
    11581 ttacgtgcgg acattgatta cgcatgggaa gaagcagata caacatacgg aaaactagga
    11641 gttaaagtgt ggatctatcg tggagaaatt cttccaacta aaaaaaacac tgagaaagga
    11701 gggaaataat catgttagta cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa
    11761 tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg
    11821 ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt
    11881 acatgaaacg tggtgggaaa gtatggatta aaattttccc tcacaaatct tatactgcca
    11941 aagcaattgg ggtacgtatg ggttctggta aaggggcacc tgaaggatgg gttgcaccag
    12001 taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag
    12061 cgttacgtct agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa
    12121 tgggtggtga atcgaatgaa ggttaaagaa atcagagaat taaccactgc cgaaatgctt
    12181 gatcaagaaa aacaattaaa agaagaattg tttaatctta gattccaatt agcaacaggt
    12241 caattagaaa acactgcacg tattaaagaa gtacgtaaat cgattgcacg catcaaaact
    12301 gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca ttaagcgtat gactgaagga
    12361 agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt
    12421 accgttgctg tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat gaactattct
    12481 aagaaataca aagtgcatga tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt
    12541 atggaaactc gtccattatc agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa
    12601 gcagttatta tctaataaca cgagattttc ctatatagat tgaatgcaaa atctgaaagg
    12661 aggatacaca acgtgatcca agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca
    12721 cgtgaaattt tgacgatcaa agtccttggt ggatctggtc gtaaaactgc taacatcggt
    12781 gatgtgatcg ttgctacggt taaacaagca acgccaggtg gggttgtcaa aaaaggtgac
    12841 gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat
    12901 attaaatttg atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca
    12961 cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca
    13021 ctagcaccag aagttttata atcttgagac acttatcaaa ggaggtgcga aacagttatg
    13081 tttgttaaaa aaggcgataa agttaaagtt atcactggaa aagacaaaaa caaagaaggc
    13141 gttgtactag cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt
    13201 aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg gaattgttga gatggaagcg
    13261 ccaattcatg tttctaacgt gatggtggtt gactctactg gtgtagctgg ccgagttggc
    13321 tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga agttttagat
    13381 aaataaaagt ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga aaaatatctt
    13441 aaagaagtaa ctccatcatt gatggaaaaa tttaactata gctctgttat gcaaacacct
    13501 aaagttgaaa aaatcgtcat caacatgggt gttggtgatg cggtatcaaa cgctaaaaac
    13561 ttagataaag cagttgaaga attaactttg atcactggtc aaaaaccaat gattacaaaa
    13621 gctaaaaaat caatcgctgg attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt
    13681 acattgcgcg gagaaagaat gtacgaattc ttagacaaat tggtttctgt ttcactacca
    13741 cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg aaactatact
    13801 ttaggtatta aagaacaatt gatcttccca gaagtagatt atgatttagt agataaagta
    13861 cgtggtatgg acatcgttat tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg
    13921 ttgacacaat taggcatgcc attccaaaaa taattaaagg aggcgaacta cgtggctaaa
    13981 aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc ttatactcgt
    14041 tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt tccatctttg ccgtatttgc
    14101 ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa
    14161 gtaaattcgc ataagaagga ggtaaaagat caatggtcat gacagatcca attgcagatt
    14221 ttctaactcg tatccgtaac gcaaacatgg ttaaacatga gtctttagaa gtacctgcat
    14281 caaaaattaa acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg
    14341 aatacatcga agatgacaaa caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg
    14401 gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca
    14461 aagctgacga agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt tcgacttctg
    14521 aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt
    14581 acgtatggta atcaagtaga atctacaagg aggtgtcttt aagtgagccg tatcggaaat
    14641 aaagtcgttg ttatccctga aggagtaaca gttactcaag acggaaacaa cgttacagtt
    14701 aaaggaccaa aaggtgaatt gactcgtact ttttcagcag atatcaaaat gaatatcgaa
    14761 ggaaacgaag taactttcac tcgtccaaac gacagtaaag aaatgaaaac aattcatgga
    14821 acaactcgtg cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg
    14881 ttagaactaa tcggggttgg gtaccgtgca caattacaag gatcaaaact tgtattgaac
    14941 gttggttact cacatccagt agaaatcaca ccaccagctg gagtaactgt tgaagtacca
    15001 gctaacacac aagtaattgt taaaggtgcg aacaaagaag aagttggcga attagctgca
    15061 aacattcgtg gaactcgtcc tccagaacct tataaaggta aaggtattcg ttatgttggc
    15121 gaatatgtac gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa aattgcatag
    15181 aagccagcag caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa tacattttta
    15241 cttgtgccgc agcttctatt tttcgaaacg tatttactgc ttgaaatagt agtacaataa
    15301 aaataaagag gtgacaattg tgattacaaa accagataag aacaaaacac gtcaaaagag
    15361 acatcgtcgt gtacgtaaca atatctctgg tactgctgag cgcccacgct tgaacgtttt
    15421 tcgttctaac aaaaacatct acgctcaaat tattgatgac gtagcgggtg taacgctagc
    15481 aagtgcctct accttggata aagaaatttc aggtggaaca aaaacagaag cagcacaagc
    15541 tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag tagtctttga
    15601 ccgtggtgga tacctttacc atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa
    15661 tggactagaa ttttagaaga aggaggaata ccattcatgg tttatatcga tccaaaacat
    15721 ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga
    15781 cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca aaaacggaca cgtaggattt
    15841 ggtacaggta aagctcaaga agttcctgaa gcaatccgta aagcaattga agatgcgaag
    15901 aaaaacttag ttgaagtacc tatggttggt tcaacaatcc cacacgaagt aatcggtgct
    15961 tttagtggtg gtagaatcct aatgaaacct gctgtagaag gttctggggt tgctgctggt
    16021 ggaccagttc gtgccgtatt agaattagct ggggtagcag atatcacttc taaatcttta
    16081 ggctcaaata caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa
    16141 cgtgcagaag aagttgcaga acttcgcggc aaatcagttg aagaattaat cggataagga
    16201 ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc
    16261 aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa gattaacagt tcagtggtta
    16321 agccttctaa tgaagcaatt aaaggcatga tcaatactgt ttctcaccta gtggacgttg
    16381 aagaaattta atttagaaaa atcataacta gaacattaag gaggtgccaa acctatgaaa
    16441 cttcatgaat taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt
    16501 acttcatctg gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa agctcgttca
    16561 ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac cattgttccg tcgtttacca
    16621 aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag tcattaatct tgatgtattg
    16681 aaccgctttg aagacggagc tgaagtaact cctgttgctt tagttgaagc tggaatcgtg
    16741 aaaaacgaaa aagctggaat caaagtatta gctaatgggg aactaaacaa gaaattaact
    16801 gtgaaagcag caaaattctc taaagcagct caagaagcaa ttgaagcggc tggtggatca
    16861 atcgaggtga tctaatgttt agactattaa aagatgcttt taaagtaaaa gacattagat
    16921 cgaaaatcct atttactgta tttgtgttgt ttgtatttcg cctaggtgca catattactg
    16981 taccaggcgt aaatgctgct tcattgcaaa acctttcttc tttgccattt ttcaacatgt
    17041 tgaacttggt aagtggtagt gcgatgcaaa acttttccat tttctcaatg ggggtttcac
    17101 catacattac tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg
    17161 ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg actcgatatc
    17221 tgacattagt attggcattt atccaatctg tcggtatcac tgctggattc aatcaatacg
    17281 ctcaattagg tttcgtgaat aatcctaacg tagcaactta cgtaacgatc ggtttgattc
    17341 taacagccgg tacgatgttt gttacttggc taggagaaca gatcacagaa aaaggtatcg
    17401 gaaatggcgt atcaatgatc atttttgcag gtatcatttc tagattgcct gaaagtatca
    17461 gagaaattta tgaagattac tttataaata ttgatccttc agacatatgg aaatctgcta
    17521 tttttgtggc cattttacta gtagcggtct tgttgatcgt gacattcgta acatttttcc
    17581 aacaagctga acgcaagatc ccgatccaat atacgaaacg cgtagctggc gcgcctacaa
    17641 gcagctatct gccgttgaaa gtaaatgcag ctggagttat tccagttatc tttgctagtt
    17701 cgtttattgc tacgccaaat gctgttttac aagcattgag cggtagattc ggttctgaaa
    17761 actggtacaa agtgatgatg gagatcttta actataatac agcacctggt gctacgattt
    17821 atacgattct gatcgttgcc ttcactttct tctacgcctt tgttcaagta aaccctgaga
    17881 aattagcgga aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc cctggtaagg
    17941 ggactgaaga gtatgtctca agattactga tgcgtttaag tacagttggt gcagtcttcc
    18001 ttggtttagt ggcattgctt ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa
    18061 tcggattagg cggtacaagc ttactgatcg ttatcggtgt agctttagaa actgcgaaac
    18121 agttagaagg cttaatgctg aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt
    18181 tgaggtttcc cctcaacaca cgttctcaca catttattag gagggaaaac aatgaacctc
    18241 attttaatgg ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct
    18301 tatggtattc cgcacatctc aacaggagat atgttccgcg cagcgatgca aaacgaaaca
    18361 gctcttggcc tggaagcaaa gtcttatatg gataaaggtg cattagttcc tgatgaagta
    18421 acaaatggaa tcgtgaaaga gcgactagct gaaccagata cagagaaagg cttcttatta
    18481 gatggctttc cacgtacgct agatcaagca aaagctctgg acgcaatgct aaaagatttg
    18541 aataaaaaaa ttgatgctgt catcgatatc catgttggtg aagaaatctt ggtcgaacgt
    18601 ctagccggcc gatttatctg cagcaattgt ggagcaactt accataaaat tttcaaccct
    18661 acaaaagttg aagacacatg tgatcgttgc ggtgggcatg aattctatca aagagaagat
    18721 gataaacctg agacggttaa aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc
    18781 ttggcttatt ataaagaaca aggtttgcta aacactattg atggtgctcg tgaaatcgat
    18841 gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc acgattgccc gtagccgact
    18901 tgcattatgc agttactctt gtcaagttca ataaattatg atagaatatg tcagtccgac
    18961 ttctagagaa tcctctagat gatccatttt gagaattttg aggtgggaac tgcagtttcc
    19021 gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga
    19081 agatatgatt gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt
    19141 tgaactagaa aacggacacc aagttcttgc tactgtttca ggtaaaattc gtatgcatta
    19201 catccgtatc ttacctggtg acaaagtaac ggttgaattg tctccatacg atcttaaccg
    19261 tggccgtatt acttatcgct ttaaataatt gtactccgta aaactacagg aggtattatc
    19321 atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa
    19381 ggacgcgtta tggtgatttg cccagcaaat ccaaaacata aacaacgtca aggataattg
    19441 gaggtgctat aaatatggct cgtattgcag gagtagatat tcctcgtgat aaacgtgtag
    19501 tagtttcact tacttatatc tatggtattg gtaacactac tgctaaaaag atcttagctg
    19561 atgctggtgt atctgaagat gttcgcgttc gtgaattaac gaatgaacaa acagatgcta
    19621 tccgtgcaga agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga
    19681 acatcaaacg tttgatggaa atcggttctt accgtggaat ccgtcatcgc cgtggattgc
    19741 ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg taaaggccca gctcgcgcta
    19801 tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa
    19861 aagtgaatcg taaacgccgt gtgaagaaga atatagaagc aggtattgca catatccatt
    19921 ctacattcaa taacactatc atcatgatca ctgatactca cggaaatgca ttagcgtggt
    19981 catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa
    20041 tggcagcaga aactgctaca aaagcagcaa tggaacacgg attaaagact gttgaagtaa
    20101 cagttaaagg acctggttct ggacgtgaag cagcaattcg ttcattacaa gctacaggtt
    20161 tagaagtgac tgcaattcgt gacgtgactc cagttcctca taatggtgcc cgccctccaa
    20221 aacgccgtcg tgtttaatga gtgccgctca ttttgagttt tatcagtaac gtcattgaac
    20281 aagacacttt tcgttttgaa aggggtaaag ataagaatga ttgagttcga aaaaccaaga
    20341 atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca tcgttgaacc tcttgaaaga
    20401 gggtatggga ctactttagg gaattcccta cgtcgtattt tattatcttc tttgccaggt
    20461 gctgccatca ctaatattca aatcgatggt gtactgcatg aattctctac catcaaaggt
    20521 gttagagaag acgtcactca aatcatcttg aacattaaag gcttagcgct gaaaatgtat
    20581 ggccaagaag aaaaaaccct tgaaatcgat attaccggac ctgctacagt aactgctggc
    20641 gatattatcg ttgatagtga tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc
    20701 tctgaaggag ctacgttcca tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa
    20761 gcagatgaaa ataaaaaaga agatatgcca attggcgttc ttccagtcga ttcaatctac
    20821 acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca ccgtgaagat
    20881 tttgataaat tgacgatgga gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg
    20941 agtttgtctg cgaaaatcat gactgaacat ttggatattt ttgtgaactt aactgatgaa
    21001 gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa
    21061 atgacgatcg aagagttaga cttgtctgtt cgctcttaca attgtttgaa acgtgcagga
    21121 atcaatactg tacaagaact tacaaataaa tctgagcctg aaatgatcaa agtccgtaac
    21181 ttaggacgta aatcattaga agaagttaaa gcaaaattgc acgacttagg tttaggttta
    21241 cgtaaagaag attaaaacct tttacgaagg agggaaacca acgtgagtta tcgtaaacta
    21301 ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt taacaactga tttattaatc
    21361 aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa
    21421 atgatcactt taggtaaacg cggagatttg catgcacgtc gtcaagctgc ggctttcgta
    21481 cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga atcagcttta
    21541 caaaaactgt tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc
    21601 attttgaaga cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc
    21661 taaaatcaca ctaataacat cactttatag atgattcttt tagagtgtta tgatgttgga
    21721 agatattttt ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa
    21781 gcactcatca taaagtgttt ttttatacct tcaattacct gtcatgacag atatgtttga
    21841 aggcataaaa aaacatttta agcgagaaat aattgaaaat tttgtctttt tttgatagtg
    21901 ttagtaggaa ctttaggatg aagaaggagt aatcatggag ccaattattg aattagaaaa
    21961 gataaattat aaataccagc cagatgatct tcgccccgca ttgaaagatg tctcctttac
    22021 aattgataaa ggtgagtgga tcgctattat tggacacaat ggttcaggaa aatcgacact
    22081 tgccaaaacg atcaatggat tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca
    22141 aatattggat gaagaaaata tttggacgat ccgacagatg gttggaatgg tttttcaaaa
    22201 tccagacaat cagtttgttg gttcgacagt agaagatgac gttgcttttg gtcttgaaaa
    22261 tcaagggatt cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag
    22321 aatggctgag tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt
    22381 tgccattgcc ggcgtggttg ccttacgacc tgatatcatt attttagatg aagcaaccag
    22441 tatgctagat ccagaaggtc gtgaagaagt gatctctacg atcaagaaga tcaaagaaga
    22501 aagtcaattg accgttatat ctatcactca tgatattgat gaagcagcta atgccaatcg
    22561 gatactagtg atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc
    22621 tgctggtcct gaactgatcg acttaggtct agatctacca tttcctgaaa aactgaagag
    22681 cgcactgaaa gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag aaaggatggt
    22741 ggattggtta tggacatccg ttttgaacaa gtagacttca cttatcagcc gaatacacca
    22801 tttgaacaaa gagccttatt tgacatcaat atgacgatca aagaaaacag ttatactgca
    22861 ttagtagggc ataccggaag cgggaaatca accttacttc agcatttgaa tgcgcttgtt
    22921 aagccaacaa gcggtacagt ccatattgga gaacgagata ttcagccgga tacggataat
    22981 aagaatttaa agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag
    23041 ctattcgaag aaacggtagc aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc
    23101 gaagaagaag cactagtcct agcaaaagaa acattagaac aagttgggct ggatgaaagc
    23161 tatttggaac gttcgccatt tgaactttca ggagggcaaa tgcgtagagt agcaatcgct
    23221 ggtgtgcttg ccatgaggcc ggaagtactt gtattggatg aaccaacggc aggacttgat
    23281 ccccagggca gaaaagaaat gatggagatg ttttggcgtc ttcataagga acagcagatc
    23341 actattgtat tagttacgca tttaatggat gatgtagcca attttgcaga ttatgtctat
    23401 gtgttggaaa agggacgaat cgttaatagc ggggagccgc aagaggtctt tcaaaacatt
    23461 gaatggctaa aagaaaaaca actaggcgta cctactgcca ccgagtttgc agaagaatta
    23521 atggctaaag ggatgaactt tgctaccttg cctttgacag ctgaagaatt agcagacgct
    23581 atcgtccatt atgctggagg aaatgcccat gatgaataaa ttgatatttg gtcgttatat
    23641 gcctggagat tccttgatcc atcgattgga tccccgagct aaattattag ccagttttta
    23701 ttttatcgga atcatttttt tagcaaataa ttgggaaacg taccttttgt tggcattttt
    23761 cacattactt tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg
    23821 cccattgatc tggctaatcc tcttcactgt agctttgcag gtcttgttta caagtggcgg
    23881 agaaacttat tggagttggg gtgttttcca tatcactgaa tttggtgtga tgaacggcct
    23941 ctttattttc tgtcgattcg tgttgatcat ttttatgtcg actctactca cgcttactac
    24001 accgcctttg gaattatcgg atgcgattga gtacttgctt cgtccattga aagccgtacg
    24061 gttcccagtc cacgaaattt cattgatgct gtcgattgct cttcgttttg tgcctacatt
    24121 gatggacgaa acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg attttggtga
    24181 aggaaaccta gttcagaaaa tgaaagcagt cattcctttg ctgattcctt tatttgtcag
    24241 cagcttcaac cgtgcggaag atcttgcgac tgctatggaa gcaagaggct acaaaggtgg
    24301 ggaaggaaga acgaaatatc gtgtattgca ttggcataat agagacacga tggttatcat
    24361 tgcttttgta ttattaactg tgctactgat ctttttaaga ggataaacgt tcagtgggcc
    24421 gctatctatt cttttcttgc tgatctgttt gcaagaaagg atgcaagcag tgtatgacaa
    24481 gaaatattag ggtctgtggc agactaaggt cacaggcctc tttcttttat ctgaaaagaa
    24541 agaacgaaaa aattttatag aaaaatcatt attccagata accttttagg aacaaaggag
    24601 agaaaaacat ggtacgttac aaagcaatca ttgcgtacga cggaacaaat tttaatggat
    24661 tccaaaaaca gccaaatggg cgtactgttc aagaagaagt agaaaaaaca ttacaaaaaa
    24721 tggcaaatgg aaaagaaatc acggtctttg gttcgggaag gacagatgcc ggagtacatg
    24781 cgatcgggca agtaatccat tttgattatc cagaggagcg tccgttagaa cgaatgcgtt
    24841 tcgcgctaga tacccagtca ccagaggata ttgctgtaag acaagtagaa attgtttcag
    24901 aagacttcca cgcaagatat ttggtcaaag aaaagacgta tcaatttcga gtggatatag
    24961 ggaaaccacg cagtcctttt cgacgccatt atgcaagtta ttttccttac ccacttgatt
    25021 tggaaaaaat tcagcgggcg cttcctgatt tattagggac tcacgatttc acctcttttt
    25081 gtgcatcagg aagctcgata gaggataaaa tacgtacgat ctacgaggct aagatggagg
    25141 taaatgaaac aaaagacgaa ttgttgttta catttagggg aaatggcttc ttgtataaga
    25201 tgatacggat cttagtagga acgcttttga aaattggcaa tggccgattg cctgaagata
    25261 gtatcccaga tattattgcg aaaaaagatc gtaatgcagc aggacctaca gcacaccctg
    25321 aaggacttta tttgtatgaa gtgaaatatg aataagaggt tatgatagaa gtggtcagct
    25381 tcaagaaata agaaggtatt tacgaaaatt acttctcaaa attttgcaaa atttcggctt
    25441 attttcgaag aagctacttc tggaacaccg tttaaggaag aggtcgtgaa atcagcgttt
    25501 tgacctgagt attagagaaa caaacgggaa aatagctcct catattttgc cgtttgttga
    25561 gctaataccg aaggtcagct gattgaacac cgtttaagaa agaggtcgtg agcctgtcat
    25621 ttaactctga gtcacaaaga gcaatttcaa aaaacagctt ctcatctttg atgaatatcg
    25681 tgacttgtgg acgaggcgtt gataagtgaa tcatcttttt gttacctatt ttttctgtaa
    25741 ccaaaaaacg atggaacgtt gttttgattg cccttggtgc ggacagtctg aacaggttcc
    25801 atcgtttctt tattttctac gtcttcttct gtgtgtgttt cgtggttgtt cttctattgc
    25861 tggatcaatc gtgccataaa aggggcgatt gcggtcaagg taccagacat aaagaataaa
    25921 caatgcaata cataggacaa aaaggattgc acctatcgta aatccttcag gcaagtaact
    25981 gaaagtgaca gtatgtttac ctgctggaac ttttatcatc aagaatgcgt ctttgaaatt
    26041 gctgaccttg actggtttcc catcaatttt agctttccat cctttgtcat aaggaatagt
    26101 tgtcaccaac atggaatctt cagcggtatt tacagtccca gaagcttttc tgccagtggc
    26161 ttccatttcg acaccttttt ctttgatctg attcattgcg gcttgatagg ctacaacatc
    26221 tagaccaacg actttaggct ccatgaaact tatttctttc gtcccataaa aacttgcagt
    26281 aaatgtcact gttgtctctt gaggataata gccaagatca tagtattgac cagaaatgtt
    26341 gatttgcgtt tttcgcgatg taccattgac agtgatcgtc gcactagaac tttctaattg
    26401 cgcaaaattg gttgggaaca aacttaagta agcttgtgta tttgcaggca catggacttc
    26461 ccaagtgagc tcttttggct ggttatctgc tgtttctgag taagtgacgc cagtcgttgt
    26521 ctttgtgatc gtcgtattca actgattctt caaagtaggt gtatagaatg taaaatactg
    26581 ttcatttaga ccagacaatg tattgatcaa gtttgtctga ctggtcaaat tatctgtcac
    26641 tggctgctca acatcattaa cggacaacgg tgcagtaaat gctagtggca atgcattgac
    26701 attctgatac agtgtatagt cgccgctttg accagcttgg cggaagccat atttactgaa
    26761 cgtgctttcg tttttagaaa tgttatattt gattcctgta aacccatcca tcagcaaagt
    26821 attattgtta taacggatat tcaaatttgt ccctcttgaa cggaaaccta atttatccag
    26881 gtaagaggaa gaattacgat ttcttataga agaaaacaaa ctgataccac tgtaaccata
    26941 gttgatcgaa tcgtttgcag atactggatc cagattttcc aagcggtaaa acgagtcgga
    27001 atcttttttt gtttcattta caagagcttt caagtcagga taaggatcag tatataaact
    27061 gcgggaagca taattccaat catcaagaat gccatggatc atactgttcg tattgaccag
    27121 tgcttcagca gacatgatca ataataacaa tatactcaga tgattgaatg aaagtttttt
    27181 atgctgataa aatcccacag caaaagcata aatccctaag aaaaccactg tcaatacaaa
    27241 ggaagtcatc gtcacatagg tgtagcttcc ttcaggtttg atgccccatg ccaccgcaaa
    27301 gagagcagct acgaccaaaa tgattcctaa caacagccca cgatcgtctt gttctatctt
    27361 ttcccatcct tttcctgcaa gcaggagcac taagaaagag aacagatagg cataacggaa
    27421 taaaaacata ttcggtgcat gcataccatg ccagaacaaa ttcaatggaa caagataaaa
    27481 acttgcgata agtatggcga aaagactgcc gtacaaaaat ttatttttca acggtacttt
    27541 ccgactggca aaataaaaca gacagaggac taatggtagc agacctgcgt aaacgaacgg
    27601 aatcgatccg tattttgtcg tgtcataaac accaatcata tttttcatga ttagatccag
    27661 ataagaagtc gcttctgttt tcaacgtagt gatctgactc agtgcctcgc catttgaccg
    27721 aagatccagc actgctggca atacaatgat cattgaagcg ccgcctgcca gtaaagaagt
    27781 gataccataa ggaacaatcg tttttttata ttctgaccag tttgtcaata gacgggcgat
    27841 aaaatataag aaagaaaata cgccaatcat aaaacccata taaaaactgg ataaaaacaa
    27901 gagtaaataa ctgataaata aaagtttcgg ccgtttaaga tccataacac gatgaatccc
    27961 caaaatcact aatggaagat aaaaaaatgc atccagccac atgatgatct ccgaatgtgc
    28021 agtgataaat gagatcaatg cgtaactgac gctaagtgca acatgccccc agcgaggaat
    28081 cttgaacgta cgtgcaagaa accaaaaagc caatccggca cagccgacct taagcaaagt
    28141 cagaacatac agtgcatctg gcatcatctg gtttggaaaa aagaacacca gtggcgtgaa
    28201 gatcccgcct agatagtaag aaattaaaga taaataattc aatcctagag aagcattcca
    28261 tgtataaaaa atactttgct ttccaagaag aacatttcgg aagctggcat gaaaattaga
    28321 aaactgtgaa aaggcatcac ttgccaaaat acttctgctg cttccaggat agattccgat
    28381 acttagataa acaagtgcta aaatcaaaaa cggaatcagg aaactagctg ctgcgtagcg
    28441 tccgttttct ctaaagaatg actttaaatt ttttttcata tttattttcc accaaatttc
    28501 tatgaagttg tattcagttt atcatatcct tgtttttctc tgcttaaaaa gagtctagtt
    28561 ttcacaaaaa aataaaattt agaataaata acttcataat ttgttcgtat aacaaaaata
    28621 aacgcaaaaa aagggtagga atcaaaagtc ccacccttaa aaatttttat taaataaaaa
    28681 accgttcact atctgaacgg tccaagtatc attcaattaa acgcgttcaa cttttcctga
    28741 tttcaaagca cgagttgaca cccaaacttt tttaggtttt ccgtcgacca taacgcgaac
    28801 tttttgtaag ttaggtttaa cagtacgttt tgttgagttc atagcgtgtg aacggttgtt
    28861 tccacttttt gtcttgcgac ctgtaaagta acaaactttt gccatggtaa tttcctcctt
    28921 tgccttgatt tggagcaata gaataattca gctcatacca gactaacata tcatatagaa
    28981 tgggaatttg caagtacatt ttcatagata caattgacaa ttgagaagaa taattgtaaa
    29041 ttaaacaagg ttatacctga aattttgcaa ggtttttgag tactttgggt aactgccttt
    29101 tatttcaagt tgcgctatga taaaatatga tagaagaaaa atgggagttt tttacccgtt
    29161 aaggaggctc attcatggct gtaaaaattc aaacgtcagc tggaaccatt gaaattacca
    29221 atgatgtaat tgcaacagtt gttggtggcg ccgcgacaga tgtgttcggt gttgtaggaa
    29281 tggccagcaa aaatcaaatt aaagataata ttactgagat tttgcgtaaa gaaaattact
    29341 caagaggagt agttgtccgc caagaagata atggtattgc tgtagatgtt tatacgatta
    29401 tcagctatgg tacgaaaatc tctgaagttt cccgcaacgt ccaagaaaaa gtgaaataca
    29461 atctagaggc attattaggc gtaactgcca attcagttaa tgtctttgta caaggtgttc
    29521 gtgtactgcc agactaggca ggagactggc tggtaactca gcagtcgatc tgaaggagga
    29581 ttatctaggt gagtgtaaca gaaataagtg caagccagtt ccaggaaatg gtccaggcag
    29641 gtgcgaatcg tctgcaagta aatgcagaat acgtcaattc attaaatgta ttccctgtgc
    29701 cagatggcga tacgggaaca aatatgaatc tatctatgac tagtggagct aaggcagtag
    29761 tagattcaac ctcagaaaaa gtaggagaat tagcagcaat gctttcaaaa ggattgctga
    29821 tgggtgcgcg cggaaattcc ggcgttattt tgtctcaatt gtttcgtgga ttttctaagc
    29881 aaattccaga tgttgtctca ttaaatgctt ctgatcttgc tgcagccttc actcatggag
    29941 ttgaaactgc gtataaagca gtaatgaaac cagtagaagg aacgattttg acagtagcta
    30001 gagaagcagc gaaagctgga gaaaagaaag caaaaagtac tgatgatgtc gttgaagtaa
    30061 tgacagctgt tgtaaaagga gcaaagcgtg ctttagctaa aactccagac ttgcttccag
    30121 tcctaaaaga agtcggtgtc gtagacagtg gagggcaagg actgctgttt gtttatgagg
    30181 gctttttaag tgcattgaat ggagaattcc aagcggatga aacatatgaa ccttctccag
    30241 cagaaatgga tgatatggtc aatgcagaac atcatcgcag tgtccaaggt caaatggcga
    30301 cagaagatat ccattttggt tactgtacag aaatcatggt aaaaatcgga gaaggaccta
    30361 cagtcgatag tacattcgat tacgaagaat tccgtaacta tttagatggt ataggagatt
    30421 ctttacttgt ggtcaatgat gacgagatca tcaaagtcca cgtgcataca gaaaacccag
    30481 gagaagtctt gaattatgga caaaaattcg gttcattagt caaaatcaaa gtcgacaaca
    30541 tgcgcttgca gcatgagacg attttggaac acgatgaaga agtgaacgag tttgcgaaaa
    30601 aagaaatacc acgtatcccg tatggtatcg tagcgatcgc tgctggtaaa ggcgtccaag
    30661 agctgtttga aagtttaggc gctaattatg ttatcagtgg tggacaaaca atgaatccaa
    30721 gtacggaaga cattgttaaa gcgattcaag aagtaaacgc agataaagtg attgtcttgc
    30781 caaacaataa gaacatcttc atggcagctg atcaagcagc agaagtagca gatatcccag
    30841 tagcagttgt tccttcaaga acgatttccc aaggattgac agctatgctt tcattcaatg
    30901 atcaagcttc tttagaagaa aacaaagaaa cgatgacaga aatgttgtct agcgttgtga
    30961 gcggtcaagt cactcacgcg atcagagata cctcaattga cggtgtagag atcaaagaag
    31021 gagacttttt gggcatgatc gatggaaaaa tcctgatctc taacccagat atcacagcaa
    31081 cagcgttaag tacactagaa caaatgatca atgaggacac agagatcgta acgatcttgg
    31141 caggtgatga aggaacagtt gaacaagcaa atgaatttgc tgagaaacta acagaaaaac
    31201 atcctgattt ggaaatcgag atccaccaag gagatcagcc tgtctatcct tatctatttt
    31261 ctgctgaata atttctgtgt cagaatatct ttctaacaca gtattcattc ataaaaggcc
    31321 ttcggttagt gatcaggtat gatcgctgac caagtccttt ttttgttaaa ggagggagga
    31381 aaaatttatg cgttctttag atgatccaat cactatgctg gcaggagtcg gagaaaaaag
    31441 agcagctagt cttgccagct tgggcatcca aacgattgaa gacttactga cttattatcc
    31501 ttttcgctac gaagatattc aggaaagaaa tctaaacgag atccaggatc aagaaaaggt
    31561 tactttgaaa ggaatcgtag tctcgcctcc tgtgatgaat cgatttggtt ataaaaaaag
    31621 ccgattgcaa tttcgtatga tgcaagaaca tgatgtgttc aacgtttctt tttttaatca
    31681 accttatcta aaagaaaaag tcattttatc ggaggaaatc gccgtatacg gtaaatggga
    31741 tgcaaagcgt aaagccttga acgggatgaa gattttaggt tctcaaacaa tggatgactt
    31801 ttcgccgata tatcacgtca acaaatcaat tcgtcaaacg acattagttg atctaatcaa
    31861 aaaagggttt caagaatacg gcacattgat tgaagaaaat ctgccaaaca accttgtgga
    31921 gaaatatcga ctgcttgacc gagcttctgc tgtaagaagt atgcattttc caaaagacca
    31981 cgaagaaaac catcaagcca aacgacgtgt cgtttttgaa gagttctttt tatttcaaat
    32041 gcgtcttcaa ggattgaaaa aagcagaaaa agcagaaacg aatggtttgg agatccttta
    32101 tgatgtccaa aaattgaaac aatttactca aaagctacct tttgaactga caaatgcaca
    32161 aaaacgtgta acgaatgaaa tctgtcggga tcttcgcagt ccgcagcata tgcagcgact
    32221 tttgcaggga gatgtaggta gcggaaaaac agtggttgcc gcgattgcgt tatacgctac
    32281 cgttacagca ggtttccaag gtgcgttgat ggtgcctaca gaaatcttgg cacaacaaca
    32341 tatggaaagc ttgcaacagc tgtttgatcc caatgaagta accatcgcat tgattacggg
    32401 gtcaacgaaa gcaaaagaac gacgtgagct ccttgaaaaa ctagagcaag gggaaattaa
    32461 cgtcgtgatc ggcacacatg cattgatcca agatggagtc gaatttcaga atttaggtct
    32521 agttattacg gatgagcagc accgttttgg cgtgaaccag cggaaagtac ttcgtgaaaa
    32581 aggctggcga ccagatgtct tatttatgac agcgactccg attcctagaa cattagctat
    32641 tacagcatat ggtgagatgg atgtatctat cattgatgaa cttccagctg gacgtatccc
    32701 gatcgaaaca cgctgggtaa gaacacctca attggacagt gtactggatt ggacgaaaaa
    32761 agaactggca agaggacatc aaatgtatgt catctgtccg ctgattgaag aatcagaagc
    32821 attagatgta aaaaatgcag tggaaatcta tgaaaaactt cgtgaactat ttgcaccaaa
    32881 atatgaagtt ggcttgttac atggtaaaat gaagaatcag gaaaaagaag taatcatgga
    32941 agccttcaaa gacaaccaga tgcagatatt agtttcgact actgtaattg aagtaggggt
    33001 aaacgtgccg aacgcgaccg tcatgctgat catggatgcg gatcgatttg gattggcaca
    33061 gctgcaccaa cttcgtggac gtgttggacg cggaagtgat gcttcctact gtatcttagt
    33121 tgctaatcct aaaaatgaac taggtgtgga acggatgaaa atcatgacag agaccaccaa
    33181 tggttttgta ctaagcgaaa aagatctgga attacgtggt cctggtgaag tatttggctt
    33241 ccgtcaatct ggtctgcctc aatttgcgat tgctgacttg gtgacggatg gaaatgtttt
    33301 ggaggtagcc agagaagagg ctgcagccct ttggcaaata aaggattggc agatgcttcc
    33361 tgagtatcag cctttagcgc aaagcctcca aatagaaaat caggataatc gttatttcga
    33421 ttagcagact gtgctatact ttatactgat gacggaaaaa ggagtggaaa caatgaaaat
    33481 tgccgtagat gcaatgggcg gagatcacgc acctcaagca attgtagaag gcgtggcttt
    33541 agcgcagcaa gattttccag aagtagaatt cttactttac ggaaaagaag cagaaatcaa
    33601 gaaatactta acaaatgaaa aaaacatcac gattatccat acagacgaaa aaatcaatag
    33661 tgatgatgag ccagtaaaag caattcgacg aaaaaagact gcatcaatgg tccttgctgc
    33721 acaagcagtg aaaaacggtg aagcagatgc tgtattttca gcagggaata caggcgcgct
    33781 actagcagca gggttgttca tcgtaggacg tatcaaaaat atcgaacgcc ctggcttgat
    33841 gtctaccttg ccagtagtag ggaaagaagg tgccggtttc gatatgctgg atcttggcgc
    33901 taacgcagaa aacaaaccag aacatttgtt gcaatacggt attttaggtt ctttttatgc
    33961 acgtaaagtc cgaggtatcg gacgtcctcg tgtcgcactc ttaaacaacg gaacagaagc
    34021 aacaaaaggt agtgaagtga cgaaaaaagc ctatgaactc cttcagaatg aaacaagctt
    34081 gaattttatt ggaaacgtag aagctcgtga gctactcaat ggtgtggctg atgtagtggt
    34141 cacagatgga tttacaggca acgctgtttt gaaatctatt gaaggaacag caatgaatat
    34201 gatgaatcta ttaaaatctg ctattctaaa tgaagggatc aaaggaaaaa tgggcgctat
    34261 gcttttaaaa gacggcttac gttctttgaa agcagaaatg gattattcca aacatggcgg
    34321 tgccgttttg tttggcttga aagcaccagt cattaaaact catggagcaa caggccccga
    34381 tgctgttcgc tatacgatcc gccaaatcca cacaatgctt gagacagatg tagttggaca
    34441 attagtcgaa caatttgaaa aaagcgaaga atagtatttg gatatctgaa gcgatccgtt
    34501 ccattgaaaa aaaggtggac attcctgaaa aatgccagta aaattagcca tggtgtaggt
    34561 aattgacaag tgtatgattc ttggaggtga aaaagttgac tcgcgaagaa gtatttaaca
    34621 aagtagcgaa aattatttca aatcattttg agatagatac cgataaagtg acggatgaat
    34681 tgaacatcaa agatgattta aaagctgatt caattagtat tatggaattc gttttagagc
    34741 ttgaagatga attcggtacg gaaatttcag atgaagacgc tgaacaaatt gaaacagtcg
    34801 gtggcgcagt agattatatc agcagccatc ttaaataatt tcaaataaaa gcaaagaagg
    34861 ataaaaagct gcatgcagct ttttatcctt ctttttttaa tctttttgac acgtaaaagt
    34921 catttttctg ataattctgg cgataaacag aaattatgtt gcaaagtaaa gcggttttct
    34981 atataattat taataattca ttacacaaag ataagaataa cctttgttat aaaacgattt
    35041 attctgtgta atgtcctttt ttattaagaa aaacttccat ttaggaagca gagaggggta
    35101 ttcgattgtc cgataaccaa ttattagaag ttcagaacct acatacagga ttccgactca
    35161 aagatgacta ttacgatgca gtagatgacg tttcatttac tttagacaaa aacgagattt
    35221 tagcaatcgt tggggaatct gggtgcggga aaagtacctt ggctactacg attatgggat
    35281 tacttgatcc taataacaca aaaattacag gtgagatttt atataacgat ctgaatttaa
    35341 tcgacctgaa cgaaacgctc tataataaaa tccgcggaaa tgatatcgga atgattttcc
    35401 aagatccgtt gtctgcatta aatcctctca tgagaatcga agaccagatc aaagaaggac
    35461 tttcctatca cacaaagatg aatgcagaac agcgtcaagc tcgtgcacta gaattgctag
    35521 aacaagtagg gattcctaat cctgcgagag ttggacgcca atatccccat gaactatcag
    35581 gcggtatgag acaacgtgtc atcatcgcaa tcgcaatcgc ctgcaaacct ccaatcgtca
    35641 ttgcagatga accgacgaca gccctagatg taaccatcca agcacagatt cttgatttac
    35701 tgaaagacct tcaggaagaa acaaaaacag ggatcatctt gatcactcat gacttaggag
    35761 tcgtagcaga aatggctgat cgagtggcag tgatgtatgg cggacagttc gttgaagtag
    35821 caggtgtaaa agaactgttt gatcatccta agcatcctta tacacgctct ttactgaact
    35881 ctataccaca agaaggaaat catgatgcag aactccatgt gatcgaaggt gtcgttcctt
    35941 ctctgaaaaa catgcctcgt aaaggttgtc ggtttgcacc acgtattcca tggataccag
    36001 caagcgatca tgaagaagag cctatccttc atgaagtcgc accgaaccac ttggtacgtt
    36061 gctcctgcta taagcatttc cactttagag acgaaaaagg agaggtgtag atgacagaat
    36121 taatcacaat caaagacctg aaagttcatt accctattcg cagtggattc ttcaatcgag
    36181 taacggatca tgtatatgct gtagatggtg tggattttat tatcgaaaaa ggaaagacat
    36241 atggtttagt gggagagtct ggctctggaa aatctactac gggaaaagcc gttgtgggat
    36301 tagaaaaggt aacagccgga caaatcatgt atgaaggaaa agatgtaacg aagcgaagca
    36361 accgcaaaaa aatgggttat aacaaggatg tgcagatgat cttccaagat tcaatgtcca
    36421 gtttgaatcc taaaaagcga gtgttggata tcattgctga acccatccga aattttgaac
    36481 ggttaagcga tcaagaagaa aagaaaaagg taaaaggact gctagatatc gttgggatgc
    36541 cggaagatgc gctatataaa tatcctcatg aattttcagg cggacaacgt caacgtctag
    36601 gagtagcccg tgctgttgct accaatccaa aactgatcgt agccgatgaa cctgtatctg
    36661 ctctggatct atctgttcaa gcgcaagtat tgaatttcat gaaacgaatc cagcaagaat
    36721 acggcctcag ctatttattc atttcacatg atttaggtgt agtaaaacac atgtgcgaca
    36781 atatcgcaat tatgtacaaa ggtcgatttg tagaaatcgg tacaagagaa gacatttata
    36841 atgacccgcg acatatctat acgaaacgtc tgctttcagc tattccgcaa atcgatgtag
    36901 aaaataggga aagccacaaa gaaaatcgac gaagagtaga acaagagtat atccaaaacc
    36961 aaaaagaata ctatgatgcg actggtcgag tctacgattt gcgtacgatc acaactacac
    37021 acaaagtggc gttgaaagat ggaggtgcaa gctaatgtgg aaaacgattc tgcgacgtgt
    37081 tttattgatg atcccgcaag ttattatttt aagtgttctg atcttcttat tagcccaagc
    37141 gatgccgggg gatccattta ccggtttgat caatccaaat caggatccgg cagtgatcga
    37201 acagatgcga caagcagctg gcttgaatga cccatggtat gtccagtact tccgttggat
    37261 cggaaatgct ttgcaaggcg attttggtca aagtttctta tacaaacgct ctgttgctga
    37321 cttgatcggc gaacggatca tcaacacttt atatctatcg atcttgacag ttgtccttac
    37381 ttatttgatt gctgtgcctc tggggatgct tgctggccgg tatcaaaatt caatcttgga
    37441 taagattgtc gtaatctaca acttctttag ttttgctgtt cctttattta tcttcggctt
    37501 gatcatgcta tttgtttttg gttatcgttt aggctggttc ccaacaagtg gatcacaaac
    37561 actaggtttg caaggtggat ttatcactca atggatagac cgtttgaaat atattctgtt
    37621 accatcaatt acccaagcat ttttggcgac tgccgtcaca atccaatatc tgcgaagtga
    37681 agtaatcgat tcgaaatcgc tcgattttgt cagaacagcc cgttccaaag gggtaccgac
    37741 gaataaagta ttcagtcgac atattttccg aaatgcagcg ttgccgatgg cttctcaaat
    37801 ggggtatgaa atcacttcgt tgattgctgg ttctgtagtt atcgaaaaga tttttggtta
    37861 tccgggagtc ggaaaactat ttattgattc catcggccaa cgggactata ccgtgatcac
    37921 atccttagta ttgattttag gaatcgcgac gctagtgggg acattacttt cagatatcat
    37981 catgagtatc gttgacccgc gtattcggat tcaataaaac caatcagaaa ggagaatgga
    38041 tcgatgagtg aaaaaaacaa tacagcagtt caagaaagta ttcctcctat ggggatacga
    38101 atgattgctc gagaatttaa aaaagataaa attgctattt tttcattagg tttgctagtg
    38161 gttttattat tagtgatctt tatcggtgcg atgctgaccg atcaagacaa agtgatgtat
    38221 gttagtatct tcgacaaata cgctgcacca ggaacagttt cgacacaagg acaaaagttc
    38281 attctgggag cagatgaagg cggacgtgat gtattaggac aattgatcat aggtgctaga
    38341 aactctgtta cgatcggatt tgcgatcacg atccttactt cgatcatcgg ggttggatta
    38401 gggatcatct ctggctacta cggagggatc attgacaaca tcttgatgcg gatcgttgat
    38461 tttatcatga ttctaccgat catgctgatc attatcgtct tcgtatcgat tatttccaga
    38521 tacaatgtct ggtcctttat attcatcatg agtgcatttt attgggtagc taaagcccga
    38581 ttattccgaa gcaagacatt atctgaagca agaagagatt atgtcagtgc ctcaaaaaca
    38641 atgggaacca atgattttaa aatcatgttc cgcgaaatca tgccgaatct aagttcatta
    38701 atcattacta atttgacgat gaactttgcg gcaaacatcg gtatcgagac aactttgact
    38761 ttcttaggtt tcggcttgcc ggcaaatgta cctagtttag gtacattgat cggttatgca
    38821 agtaacggag aagtcttagt gaatcggcaa tggatctggt tacccgcatc aatccttatt
    38881 ttagttttga tgttgagtat caattatgtt ggacaagcat tcaagcgttc tgcagatgcg
    38941 cgccaacggt taggctagta tagaagcgaa gggggaaata ggaatgaaga aaacgatttt
    39001 tggatttgca gcgctgatgt ctgctgtcgt tcttgcagca tgcgggggcg gaggtacaga
    39061 tacctctgga aacaactctg gaggcaacgg caataacaaa gaggaattaa cttttccaat
    39121 tgcaacaaaa aataatgatg atgcaatcga agacggcgaa ttagaagctg cggtcgcaac
    39181 ggacagtcag tttaaaggat tgttccagtg ggagttttac caagattcat atgatgcagc
    39241 ttttatggct ccttctcatg aaccgctatt tgcaaacgat gacgatttca gaatcacaga
    39301 cgacggagct gcaacgcttg atctagatga agatacgaaa aaagcgacaa tcaaagtaaa
    39361 agacaacgtc aaatggtcag atggtgaaga tgtaacggcg gacgatgtga ttttccctta
    39421 cgaaatcatt gggaataaag attacactgg tatccgttat gacgacactt tccgtaacat
    39481 cgtaggtatg gaagaatatc atgatggaag tgccgacaca atctccggaa tcaaaaaaat
    39541 agacgacaaa acagttgaaa tcgaatataa agaagtaaac ccaagcatgc ttcaatcggg
    39601 tggtggtatc tggtcatatg cagcaccaaa acatacgtta gaaggtgtag caatcaaaga
    39661 tatggaatca agcgatcaag tacgtaaaaa tccagtgact tttggccctt attacatgag
    39721 taatatcgtt gcaggagaat cagtagaata cttgccaaac gagcactact ggaatggtac
    39781 gcctaaattg aaaaagatca cgatgaaatc tgtaccgatg gcaagtgcaa ctgaagcatt
    39841 gaactctaaa ttatatgata tgattttcca aatgccaaca gatacttacg atacctataa
    39901 agaaattgct ggctatacaa acttaggccg tgaacaaact tcttatactt atctaggatt
    39961 caagttaggt aaatggaatg cagatacaaa gagtgtggaa tacaatccag attcaaaaat
    40021 ggctgataaa tcattacgtc aagcaatggg ctatgcatta gataataatg cagtaggtga
    40081 acgtttctat gcaggtttga gaagcaatgc gacttcattg attcctccag tattccagtc
    40141 ataccatgat gatgagatga aaggattcac acaagatcta gacaaagcga aaaaactatt
    40201 agatgatgct ggctacaaag atgtggatga cgatggttta agagaagaca aagatggaaa
    40261 taaactaaca atcaactttg cttcgatgtc cggtggggaa acagcgcaac cattggcaga
    40321 ttactatatc caacaatgga aagaaattgg tttggatgtc caatacacaa caggtcgtct
    40381 gatcgaattc aattctttct atgataaatt agaaaacgat gatcctgaaa ttgatgttta
    40441 ccaagctgct tggaatacag gaactgatcc aaatccaagt ggactatggg gaccaaatgc
    40501 tgcgttcaac tacactcgtt atgaatcaga agaaaataca aaattgatca acgatatcag
    40561 ttcaagcaaa gcatttgatg aagagtacca aaaagaagca tatcaaaaat ggcaacaata
    40621 tgcatttgaa gaagcatttg cgattccaac gttgttccgt aatgaagtat tgccagtcaa
    40681 cgaccgtgtc aaagactggg attggtctta tgatgtacca aacccatggg caaatgtttc
    40741 tgttacagca gattcacgtt cataatcact aaaaatgacc gaaaaagggc gatgtccttt
    40801 ttcggttttt tgatattttt ctgtttattt ctctaatatt caggtcaagg cgctgatttc
    40861 atgacttctc caataaacgg tgttcaaagt ccagtttctc ggtattaagt caaatttatg
    40921 caaaaatatg gaaagccatt ttcacaaaat tttttcttca tactcgaaac taaacgaatt
    40981 tttcacaact tctcgtcaat cactaggatt attaaagctt tacttacctt ttgaccgaac
    41041 catttcaaat ttgacaaaaa accagtataa taaggaagta tcgaaaaaat atccataaaa
    41101 ggaatgtgga aaatggacaa ccagctgaca aaagaactaa aagaaaacta taacatcgtt
    41161 tttcacgatc taaatttgct tgaacaagct tttacccatt catcctatgt gaatgagcat
    41221 cgcaatctgc aattatcgga taatgagcga cttgaatttt taggagatgc cgttttagaa
    41281 ctgatggttt ctcagtattt attccgttta tatcctcatt tgccagaagg aaaattgaca
    41341 aaaacacgag ctgcgatcgt tcgcgaagac agcttgtcga aatttgccaa agaatgtcac
    41401 tttgatcaat atgttttgtt gggcaaggga gaagagaatt caggaggacg tacgcgacca
    41461 gcattgcttt gcgacctatt cgaagctttt ttaggcgcgt tatatcttga ccagggattc
    41521 gacacgaccc atgcattcat cgaaaaagtg atttttccta aagtcaaagc aggtgctttt
    41581 tcacatgaga tggatcataa aacaaaacta caagaagtct tgcaaaaatc aggtgatgta
    41641 tcaatcgaat accgattgat caatgaagaa ggtccagctc atgaacgagt attttggatc
    41701 gaagtttatg tagatgatca attgattggc actggacaag ggaaatcgaa aaaattagca
    41761 gaacaagctg cggcggaaaa tgcattggca gcactgtaaa agtaagtgag ggaaagcttt
    41821 gtatttgaaa cgaattgaaa tagccggttt taaatcattt gcagacaaga cagtcattga
    41881 ttttgaaaat agcgtcacag cagtggttgg accaaatggc agcggaaaaa gcaatattac
    41941 agaggctatc cgctgggtat taggagaaca atctgctaaa agtctgcgag gcggaaaaat
    42001 gccggatatc atctttgccg gttccgatac ccgtaagcag ctgaatattg cagaagtcac
    42061 agtgatttta gacaatagtg atcattatct gccattggag tacaatgaga tcagtgtaac
    42121 ccgccgttat cgacggactg gggaaagcga gtttttcatc aataaacaaa gctgcagatt
    42181 aaaagatatc caagaattgt tcttagattc tggattagga aaagaatcgt tctcaatcat
    42241 ttctcaagga aaagtagaag cgattttcag tagtaaaccg gaagaccgtc gtgggatctt
    42301 tgaagaagca gctggtgtct tgaaatacaa gcagcggaaa aagaaagcag aacagaagct
    42361 ttttgaaaca gaagacaacc tcagccgcgt ccaagacatc atccacgaat tggaagaaca
    42421 gttaactcct ttggccgccc agagtgaggc agcaaaggag tttttacggc taaaagaaac
    42481 actgactcaa accgatgtct ctttgatggt tgctgaaatc aagacagcaa aaaaagattg
    42541 ggacaacaaa caagcacagc ttgcgaaatt caatctagaa ttaggcaaac tatctgagtc
    42601 gatccaagaa caagaatcca ttttggcgaa gcagcgtaaa gaaaatgcgc aagccgatcg
    42661 tctgatcgaa aaaaatcagc aagtattatt agatctttcg gaaaaactga agcagacaga
    42721 aggacaaaaa gatgttttgc aggagcggac aaagcataca caaaaaagct cgcaagaata
    42781 tcaaacttca ttagcagaag cacaaaaaaa ggttaaacat tttgaaaagc ttcaagaaag
    42841 cttgatgaaa gcagcagcag aaaaagaaac agagatccaa aaagctgaag caaatctcat
    42901 aaaaacacag caagaattag aaaaatacca aaaatcaacg aaagaattat tggcagaatt
    42961 aagagatcaa tatgttgatt tgatgcaaga acaagcagct gtaggaaacg aactgaagta
    43021 tctagaacgt caatatatac aagaaacagc aaaaagcaag cagacgttag ccaagcaatc
    43081 ggaagtagaa gcatcagtag accgattgat cttacaaaaa gaagaattga cacaaaagca
    43141 ggcgcagcta aaatcatctt taactgaaac aaaagagaag ttagaaatga tccaacaaaa
    43201 cgggaaaaag ttccaagaag cattagcaaa agaacagcct aaaatgtatc aattaatgaa
    43261 tcaagtccag cagctacgtg ctcgtaagaa aagccttcaa gaaatccaag aaaactattt
    43321 tggtttttac caaggggttc gattggtctt gcagcataaa cagcagcttt cgggtattgt
    43381 cggagcagtg gctgaactga tcgatgttcc tgcagatttc acgttagcta tcgaagcagc
    43441 tttaggtggg gcagcccagc atgtcatcgt tgaaaatgaa aaagatgctc ggcaagccat
    43501 cacgtatttg aaacagcaaa gaggaggaag agcaacattc cttccactga cgacaatcaa
    43561 accaagacaa ttaccggctc atatattgac acaagcagca gctgtagaag ggtttatagg
    43621 gattgccagt gaacaagtca gttatcccga tcaaatccaa actgtggttc aaaacctgct
    43681 aggaacgatc cttttagcca aagacttaac tagtgccaat gcaatcgcgc aaacgatccg
    43741 ctatcaatat cgagtggttt ccttagaagg tgatgtgatg aatgccggag gatccatgac
    43801 aggtggagcg aataaacgag gaaaccaagg cagtttgttt gtacaaaatc aagaattgaa
    43861 acagttgact agtgaatttg aagaagcaga taaacaactc caagcacaag aaaaaaaagt
    43921 tcaggagctg caacaagaaa cagctcgttt agccgaagaa caagaagttt tgcgtacacg
    43981 aggcgaacaa ttacgttttg aagaacaaga agcgacaaat cagctacaaa acatcataaa
    44041 tgaacttgaa cgttttgaaa aagaaaagca aatctcaact tttgagacac gtgaactgca
    44101 gcagtttatc gaagattacg aaaagcaaac gaacgaattg aaagacaaac aaacagatct
    44161 tgaatcgcaa cgacaaaaaa tcgacgaaga aatcaaatcg ttaagtcaag aaagcgacca
    44221 gatggaagcc agaagagcac aagtacaaag tcaaaaagca caagaacaag cagatcttgc
    44281 tgtcttgaaa gagcagttta atcatctgca gatccagctc cgcggtgccc gagtccaaaa
    44341 agcagaggca accgaacgtc aagaagcgat agaaaagcag ttggcgacat tgacagcaga
    44401 cttttctgat catgaagtca cagaagaaag tttggaaaaa caaattaacg aattatctgc
    44461 tcagcgagaa acattaaaag ccgagttggc aaaagccaaa gagcagcgag atagaaggca
    44521 aaaggagatc gataagcttg aagcagtctt ggcagagcgt aatcgtgagc aaaaagcacg
    44581 actgtcggaa caatccaaat tagaagtcca aaaagaccga gctgaaatga ttttagataa
    44641 tcatttgacc tatctgcagt cagaatatca gctgacattt gagaaagctt cccaagacta
    44701 tcaagaaaca accgatatcg aagacagtag aacaaaggtt tcatccttaa aagaacagat
    44761 tgaaaaactg ggaccagtaa acttaaattc aatcgaacaa tacgaacaag tcagtgaaag
    44821 acatacattc ttagcaacac agcgagatga tctgttagca gcgaaaaacc aattgtttga
    44881 aacgatggat gagatggatg atgaagtgcg gacaagattc aaagaagtat ttgaagctat
    44941 ccgtcaagaa ttcaaaattg tctttccaaa tatgtttggc ggcggtaaag ctgaattagt
    45001 actgacggaa ccaactgatt tgctgaaaac aggtattgag attgaagtac aacctcctgg
    45061 caaaaaactg caaagtctaa gtttgctttc tggaggagaa cgagcactta cagccatcgc
    45121 gttgctcttt tccattatcc gtgtctgtcc tgttccgttt tgtatcttag atgaggtaga
    45181 agcagcgtta gatgaagcca acgtgaaacg ttttggtcgt tacttgagcg atttccaaga
    45241 tgacacacaa tttatcgtag tcacacaccg caaaggaacg atggtcgcag cagatgtact
    45301 gtacggagtg acgatgcagg aatccggtgt atcaaaaatc gtttctgtac ggatggaaga
    45361 tatcaatgaa gaggcgaaaa tcaacgaaac gaaagtgtag gaacgaaaat gatcaaatta
    45421 gtagcaatcg atttggacgg tacattatta aatgaacaaa agctgatttc agatgagaat
    45481 aaacaagcat tagcacaagc aaaacagcaa ggggtcaaga tcgttctatg tacaggacgc
    45541 cccttagctg cgatggcgca ttatctgcaa gaattaggac tggtagatga aggtgactac
    45601 agcatcactt tcaacggcgg tttggtacaa aaaaatgata ctggtgaaat catggaaaag
    45661 aaagtgatgg aagtatcaga tatccaacgc ttatacaaac tggctcagca attggatctg
    45721 ccactagatg ttttgtctga caatgtggtt ttacagctgc ctagtgcccc aaggaagcaa
    45781 tccttgtaca atgtgttgaa taagctgctg caattccaac cagcggatat aagcgatatt
    45841 acagatgaat ttgtgcttaa taaggcagta atcgcttatg accaagaaga attagatcct
    45901 aaaatcaaag aaataccagc agaattccat gaaatgtatg agatcatcaa aacaagaagt
    45961 atgcttttgg aatttatgcc aaaaggtgtt acaaaagctt atggtatttc tttattggca
    46021 aaagatcttg gtttgaaaca agaagaaatc atggcgatcg gtgatgagga aaatgattta
    46081 ccgatgatcc aatatgctgg actaggcgta gcgatggaga atgctgtgcc tttcgtcaaa
    46141 gaagcagcag atttcgtgac agccagcaat gaagaaaatg gcgtagccaa agtgatccaa
    46201 aaatttgtat tagagtaggg ggagtcaaat gggatttttt gataaaatca agcgtgcttt
    46261 tactggcgaa gagaaaacag aagaagttga agtacaggag aagtacgaca agggtttaga
    46321 aaagacgcgt aaaacatttg gtgaacggat gaatgagttg tttgccaatt ttcgaacagt
    46381 cgatgaagac ttttttgaag aattagaaga aacacttatc ggtgcagatg taggttttga
    46441 aacagcgata cgaatcaccg aagcattacg tcaagaagtg aaactcagaa atgcgaaaaa
    46501 atcggcagaa gtccaaaatg cgatcatcga gaaacttgtg gatctctatg aagcggaagg
    46561 aatcaatgaa gtaaatgaac taaatatcca agaagacgga ctaacagtta ttttgtttgt
    46621 cggtgttaat ggtgtaggga aaacaacaag tattggtaaa cttgcccatc agttcaaaaa
    46681 cgaaggtaaa aaagtattga tggcagcagc agataccttc cgcgctggag caatcgacca
    46741 gttagttgtc tggggcgaac gcgctggtgt tgaagttgtt cgtggcaatg caggaggcga
    46801 tccagcagct gtcgtattcg atgctttgga aagagcaaag acagagcagg cagatgtttt
    46861 actagttgat actgcaggcc gactgcaaaa taaagtgaac ttgatgaacg aattagaaaa
    46921 aataaaacga gtaatccagc gggaattacc agaagcaccc caagaaacac ttcttgtatt
    46981 agatgcaacc actggtcaaa acgcgatggt tcaagcaaaa caattcaaag aaacaacaga
    47041 tgtgaccggt cttgttttga caaaattaga tggtacagca aagggcggta tcgtacttgc
    47101 tatccgtaat gaattgcatt taccagtgaa attggtcggt cttggtgaag gaatcgatga
    47161 cttggagccg tttgatccaa atgattttgt cgttggattg ttcaaaggat tactaaaaga
    47221 agaataaccc atgtctaaaa agcaggagct gtgacaaaag ttgcagctcc ttgtttgaat
    47281 gattgttttt aacatagctg tgtttttgat gccttcattt ttatacttat ttgtaagcgg
    47341 ttctcaaaca atttcgttct ttttcttaga ctttttgatt ataattagat tcatatgaat
    47401 tggaggtcac ttaatgatga attttaagca attattattg catgtcaatg cgcgcccttt
    47461 tatcgaccaa gctcgatttg gtatagaacg ggaaggacag cgggttgatc ttgcaggaaa
    47521 tctagcaaaa accgatcatc cagcaatctt tggcgatcga tcctatcacc cctatatcca
    47581 aacagatttt agcgaaacac aaacagagat gatcacccct gttaccgatt ctattcccga
    47641 attatttcag tatctcgctg ctgtttatga tgtgactgct cgttctatac cgaaagaaga
    47701 gatgatctgg ccattaagta tgccacctgc cttaccggaa aaagacgaag agatcattat
    47761 tgcaaaatta aaaaatttcg aagatgtctt gtatcgacgt tatttagcaa aagaatacgg
    47821 gaagcgaaaa cagatggtaa gcggtattca tttcaatttt gaatttggcg atgagttgct
    47881 aagaacattg ttcagccatc aggaagaatt tcaagatttt tccgaattca aaacagaact
    47941 ttatttgaaa acagcgagaa actttatgcg ctatcgctgg atgatcactt atttattcgg
    48001 tgcttcacca atgagtgaga agaactactt tttagatgaa tcacatccgc aagaacctgt
    48061 tcgcagtatt cgcaacagtg cgctgggcta tacgaatcat ccaaatgtga aagtttccta
    48121 tgcttctatg aaacagtatt tagcagatat cgagcgaatg atcgaagaag ggaaactttc
    48181 ggaggagaaa gaattttata caccgcttcg tttccgaggc ggaaagaaag tcgcagattt
    48241 agcaacaaca ggtgttcgtt atattgaatt gcggaatatc gatttgaatc cttatgcaag
    48301 attgggtatc aatccagagc aagttcggtt cttacaatta ttcctgatgt acatgttatg
    48361 gacagaagaa aaagaagact gtgaccaatg ggtagcagaa ggtacgactc gaaataacaa
    48421 agtagcacta gaacaaccat cagatcaaac agaatttcat caagaaggta gagaaattct
    48481 tgaaggaatg aagcaaatgc tggttgaatt ggattggttg gattctcttt atttagtaga
    48541 ggaagcattg actcaaatgg atcatcctga acaaacgtta gcagcgaaac tctatcaaga
    48601 agcacagcta tcgagtcagc aagaagttgc tgtggcattg ggacatcaat attataaaga
    48661 aagccatgaa cgtccctatc agttggccgg ttttcgcgaa atggaacttt ctactcagat
    48721 atttatgttc gatgccatcc aaaaaggtgt ccaagtcaaa gtattagatg agtcggatca
    48781 atttttgcgt ctgcaattcc aagaccatgt agaatatgtg aaaaatgcaa acatgacaag
    48841 taaggacagc tatatcgtcc cacttatcat ggaaaacaaa acagtaacga aaaaagtctt
    48901 aaaagaagct gggttccggg taccaggagg cgcagaattt tcatccatgg aagaagcggt
    48961 aaaagcttat ccgagatttg ccgagcaggc atttgtgatc aaacctaaat caaccaatta
    49021 cgggttaggt atcacgattt tcaaggaagg agctagtctg gaagattatc aagcaggttt
    49081 agccattgct tttcgtgaag acagttcggt tttagtagaa gaatttatgc cgggaacgga
    49141 atatcgtttc tttgtgatcg acggagaagt ccaagccatc atgttgcgag tccctgctaa
    49201 tgttattggt gacagtatcc gaacagtgaa agaacttgtt gaagaaaaaa atagtgaccc
    49261 tttgcgtgga acgaatcatc gtgcaccttt agaattgatc caattaggtg aattagaaca
    49321 attgatgttg aaagaacaag ggttgacgat cgaatcagtt ccccaagcca atcaaattgt
    49381 ttatctaagg gagaactcaa atatcagtac aggtggtgat tcgatcgata tgactgatga
    49441 attttctgaa gcttacaaaa aaatcgccgt ttctgcggta gaagcactag gagctaagat
    49501 tagtggtatc gatttgatta taccagataa agaaatcgat cctacaactg ataaaaaagc
    49561 atatgggatc atcgaagcaa atttcaatcc agccatgcat atgcatgttt atccttttgc
    49621 aggaaaagac agaagattga cgatgaatgt attgaaattg ttatatcctg aagtatttta
    49681 aaagaaaaag cttttgacag attgtcctat ttgatgtcaa tctgatcaaa agcttttttt
    49741 gcatgtaaaa atttatgtgt tttttcgttt taaaataaac gatcccagaa gctttttttc
    49801 ttttttggtt tttctttttc agctggcgaa gagttttctt ctgggtattt tttttcgata
    49861 tcaatcacag gttcatttac tgcttctttt gctgttaaga gcaatccgaa ttcctcagga
    49921 tttgcagtga cgtaatcgtt gactacagtg aaacggctat tgtttttcgt tgctagctgg
    49981 atatattggt tttggacaga aaggggtaga gccccattca agagtatgga agcatctgga
    50041 tgttttttga gttcttttaa aaagttttct ttgttttttg gttggatcat ttcagcaaca
    50101 gtcatactaa gatagcagcg ttccctgaat gtccctaaat atttatgctg ttcttcaggg
    50161 ttcactaaag gtgtaccata aagtccctta tccaaatggc tttttacatc atcttgtgcc
    50221 ataaaaaata ccctccttct tctctttgtc tttcttacga ttatagcata ttcccaataa
    50281 tacctaaaac atatgaaaaa gcggttgctt ttgttcaacg attcacttaa aataagaatg
    50341 aaaatcattc tcaattagaa aggtggattg gatgcgctac ggacgactag tagcattatt
    50401 agttatttta agtagtattt cattgttcat cggagtatcg aatgtacaat tgtcagaact
    50461 gctccagtta gatgggcaaa gcttacaaat cttgctagtg actcgtttgc cacgaacgat
    50521 ttgtttgatc cttgtgggag cgaccagtag tatttgtggg ttgatcatgc aacatttgac
    50581 ccaaaataaa tttgtttcac caacgactgc gggcacgatg gatagtgcgc gtttaggtat
    50641 tttagtggct atgattttcc tacctggtgc atccttgatt gtccgatcct tgacagcatt
    50701 ttgctttgct tttgcaggaa cattgctttt tttatccctt acgcgattgt tcctccagaa
    50761 aaatcaattg atgcttccgt tgattggagt catgttaggg aatatcattg gttcagttgc
    50821 tacttttttt gcttatcaat tccagttggt acaaaatatg acttcatggc ttcaagggaa
    50881 ttttgcgact gtgatacgag gaaattatga gttattgtat gctgttgttc cattgttgct
    50941 cttggtttgg ctatttgctt accgatttac gatcattggt ctgggcgaat cgtttgcagt
    51001 aaatctggga atagattacc agaagatgca gtttttaggc attggcttag tatcattggc
    51061 tagtgcagtc atgttgatca tggtaggaag cattcctttt atcggagtga tcattccgaa
    51121 tcttgtctct agaatatatg gagatcaagt gcacaaaaca attggcgtaa ctgctttagc
    51181 aggtagtttg ttcttagtat tttgcgatct tgtcgctcgc gtgttgattt ttccttatga
    51241 aataccagtc agtgtgatcg ttggaatcat cggtggggct atcttccttt atcttctgat
    51301 aaggggacga cgatatgctt aaaaaaaagc catctatttt tattggtatg cttgttcttg
    51361 ttttatttgt tacaggaatg tacttgacga tccatacata tggaaactgg cattttgcat
    51421 gtgcattgag gagtaagaaa atttttgcat ttatccttgt ctcgctagct gtcagcagtg
    51481 ccacaatcag ctttcagaca attacgcata accagttttt gactccttca gtattgggac
    51541 ttgagtcgtt atatgtcctt gtccaaacgg tccttttctt ctttatcgga ggagtgacga
    51601 tgcttcagca gacaggtaca gtttttttct tattgaacgt tgggctgatg gtcgggctga
    51661 gtctattgtt atttttcttt cttttgaaaa aaatgagagc gaatcttttt ttattactga
    51721 tgtcagggat gattttaggt acgctttttg gtagtgtcag taccttttta caagtcatca
    51781 tggatccgaa tgaatatgat ttgcttcaag gcagattatt tgcaagcttt ggcaatatcc
    51841 agagtcagta tcttttgcct gcttctttga tcattatcgg ctgctggtac ctgttatttc
    51901 gatatcgaca tgtgctagac gttttccatc tcggaaatga acaagcagta aacctcggga
    51961 tagacacaga tcgttttgaa tttttgattt tatgttgtgt cagtcttttg acagggacat
    52021 caacggctct tgtgggtcca gttacttttt tagggtttat tgtcgcaaat atcagttatc
    52081 gtgtcttttc tacttatcgt catggtcagc tatttatcgg aagctttctg gtagctatgc
    52141 tgtttctgac aggaggacag ctcgcagtag agcagatttt cggctggaat acaacagtga
    52201 gtgtcgtcgt tgaatttgtc ggaggaattt attttgtcac aaaattgatt cgcgaaagga
    52261 agagtatcct atgattgatt tacaaaaggt caataaactg taccatggaa aatcagtggt
    52321 taaagatgta cagttaacga ttcctgaaaa gaagttgact gcttttatcg gtccaaatgg
    52381 tgctggaaag tcaacggtgt tgtcgatgat cagtcgtttg ataccaaaag atacaggtga
    52441 aatctattta gaccataacg aagtaaaggc ttggaaatcc aatgaattag ccaaaaaatt
    52501 atcgatccta cgacaatcga atgaaataaa tgtccaaatc accgtgaaag aattagtaga
    52561 atttggacga tttccttata cgaaaggaaa attgacgaca aaagacgagg aaatcgttca
    52621 ggaatcactg gaacaccttg ggttagaaga aatagcggat caatcgattg acactctttc
    52681 aggcggtcaa ctccaacgtg cgtatattgc aatggtgcta gcgcaagaca cggattatat
    52741 tttactggat gaacctttga acaatctaga tatgaatttt gcggttcaaa tcatgcagat
    52801 tttaaaaaat ttagtacaag aaatcgggaa aacaattgtt attgttcttc atgatatcaa
    52861 ctttgctgca agttatgcag acgaaattgt tgcaatgaaa gatggacagt tatttacgca
    52921 aggaacgacc caagaaatga taaaaaaaga tgtattggat gaattatacg aaatggacat
    52981 acggatttgt gagattgaag gccgcagatt ctgtctttat ttctaaaact aacattagga
    53041 gagaagagat gaaaaaatta gtagtaggta tcttgattct agcatcattt ggtttagcag
    53101 catgtggaaa tagcaacaac accagtcagg cagacacaaa aaaaagcagc acacaaactg
    53161 aaactacatt gacgataaca gatagtaatg gagaccaaat cgaggtgcca aataatccag
    53221 aaaaagtcgt cgtattcgac aatggttctt tagatacgat ggatgctctt ggtgttggag
    53281 ataaggtagt tggagcagct acaagcagcc ttcctgagta tctttcatcc tataaaaaag
    53341 tagaatcagc aggtggaata aaagaaccag acttagaaaa aattaatcag cttcagccag
    53401 atctaatcat catttctggc cgacaaagag attttcaaaa agatctaagt caaattgctc
    53461 cgaccatttt cctttcttta gatgcgaaaa atccatggga atctttccag caaaatgtca
    53521 ctgctttagg ggagatcttt ggtaagcaag aagaagctaa aactcaatta gaggaattat
    53581 ctagtgcaat cgatcagacg aaaaagaaag cagaagctac tgacaaaaaa gcactagtca
    53641 cacttgtgaa tgaagggcaa ttatcagcct acggatctgg ttctagattc ggtttcatcc
    53701 atgatttatt tggctttgaa caagcagatg atcaaatcga agcatccact cacggtcaaa
    53761 gtgtttctta tgaatatgtg ctggaaaaaa atccggatat cttatttgta gtagacagaa
    53821 caaaagctat tggtggagac gacagcaaag atgatatttc tgccaatgaa ctggtagcac
    53881 aaacgaatgc tggtaaaaat cagcagatca tctcccttga accagatgtc tggtatttaa
    53941 gcgggggcgg tttagaatca atgaaactga tgatcgaaga tgtgaatcaa gcatttaaat
    54001 agcataaata acagaggatt aaactctcat actatgtaac aataatgttt cttacattct
    54061 taaatagcaa gagcctatcc tcaaagaagt tatcaaaaac catccgtaaa atcgtattga
    54121 tgattttacg gatggttttt atttatttga gaaatctttt ttcaacgtga attcattatc
    54181 tacatcatac gggttgattc tagaatgggg agtggcaaaa aatagtgtga tccatttttt
    54241 cagcatcaag atcgcctcct ttgatttgaa tcaaaggtac aaagcgaaac ttagataaaa
    54301 cttaaattta gatattttta ttttgtaccg atctcttctt taaacgatgt tccatctgct
    54361 gcttctcggc aataagtcca ctagactaga aaatatgaga agctattttc tgtccagcgt
    54421 tcttattact caaaacaaat cgcagttgtc acaatctctt ctaaacgatg ttccatctgc
    54481 tgcttctcgg caataagccc actagactag aaaatatgag aagctatttt ctgtccagcg
    54541 ttcttattac tcaaagcaaa tcgcagttgt cacaatctct tctaaacaat gttcaatcag
    54601 ctgaccttcg gtattagctc aaagcactga tttcacaacc tctatttagg catgtgtctc
    54661 agcaagcggt tgatagttga tgatctgcat ttttcgataa aaatcggcgg gaagcttact
    54721 ccaccgtttt agtaattgat agaaataact ctcttctgct ttgattttat actcgcttaa
    54781 tatcaatcca aaactattcg ttttttttga agctaatttt ttataaatat ccaatgcttt
    54841 ggttttatct tcattgacaa agagtgtttt tacttcaata taagaaccct cgatattttg
    54901 tgtttcttcg ataaaataga tgttttttat atattccttc atagtgattc ctccaaagaa
    54961 gtcttttatt tgataagttt actctaccga agagttgtta ataatccgtg aacaaaaagt
    55021 aattcttttt ccaatagttg ttaaaggttt tttatttttt atagtatttt ttgttattta
    55081 agaaaggaat aaggttaaac aaataacagg ttgctttttg ggtctttttc cgttcaagaa
    55141 actgaacgaa gaaaagggaa agttgtctct tttgtgataa gagaaatcat aataaacgaa
    55201 tgacttactt tccaataaat tgagaacagt cagaaccaga caaattagtt gcatctgatg
    55261 aataaagtga ttattctatg acatgaggtg atacagatga aattagaagt gacaaaagaa
    55321 gcacaagaag tattgaagaa taaattagaa gcagacgatc aacttttatt agatatcgaa
    55381 aacggtgacg gtccatttgc agaaagccaa gtcagttgcc aattggatac ggcatttcga
    55441 ttgatcattg tcaaaaaaga tacacaaaca gatctaagtc tttattctgt agtggctgat
    55501 acaccagttg gaccaatcaa aatcaaagac agtgcaatac tttacatgga tgatccatcc
    55561 acacttgctt tagaaccaac ttataatagt ttacaattaa aaggaccaag cggcttgaga
    55621 aaaggcaact tgcaagttgt tcgaaaagac tagagggata aaatgggaaa attggcgatc
    55681 atcagcgacc tacatgtgga catcaataaa ttcggcgaat cagagctgat gcagctttgg
    55741 caagttttgc aaaagcacaa gatcactcgg cttcatctag ccggagatac ggctaataaa
    55801 gtagaccgtt gcctagctgt tgtcgaattt ttcgtcaata aaggattatc tacgacattt
    55861 catttcggca atcacgagtt ggcagatgtg cccaatgaac agatgatgga ccattttcca
    55921 gacccacatt ttttaaatga acggtatatt gctttaaatg agcagaccgt tcttttaggg
    55981 atgaatggct ggtacgacta tcagttttcc gaattgaaag attacgaggc aatcgtacga
    56041 atgaagcgtt tgtattggta tgatcggttc atccagcgtg ggaaaacaga tctcgaggtc
    56101 aatgcagaaa tgcttgcagc tgcaaaaaat ttgctagacc agctggaagc aaaaaaactt
    56161 gaggtgattt tatccactca tttcgttcct aaacgggcat ttatcatgta tcaatctgct
    56221 ccttatgagc gttggaacaa attaaatgca tttttaggat cagaatcatt cggagaatta
    56281 cttgaccgtt attcaaatat caaacaagta gtttttggcc atacgcatcg tcgctttgaa
    56341 gataaatcca tcggtcacac gatttacagc tgcagacctt ttggttatta ttatgaatgg
    56401 cagctgaccc gagattttgt cttgaaagaa cagttgctag acagctatga tcctatgaaa
    56461 ttacgtgtcc tcttgcgtgc ccatcaccca gcttttcagg cctatcaaac acaacatttg
    56521 caagaggaat ttgaacaagc gatgacaatc gttccgtatt gagacagcat tagtaatgtg
    56581 taaaagaaaa taccttgtaa aacataagga taagtaagag cagtagatcc atttatcgtg
    56641 gatatgctgc tttttttgcg gaaaataatc agcgaatgtt tatttgatcg gatgatttgt
    56701 aactcgcaga gtactagatg caagcgtgta ttcttttttt gaacgataat cacaaaaaaa
    56761 aatataatat acatttttta tttaattata attaatattg attgaagtct aggcagtcaa
    56821 gtcacatttt gatagatagg agggggactt acgaattatt ttaaaggcta tgacagctgt
    56881 atttctagat tgtgtttgca tcaagttgca ataaaaacta gaaatcaaat cgggctgata
    56941 atcatttata actaatcgat acataggacc aaatataggt ctatgttctt ttatttcata
    57001 agtatcgtaa aacagaattt tgctatggat taatccaatc gactgatatg tttagtaaat
    57061 aaagaaagag gaatgtcaat gatgaaaaaa gaaatcgtat taacagcagc aatgatgtta
    57121 ttcagcatgg tagcaagtac gacttttgtt tctgcaactg aagtataccc aaaggaatat
    57181 aacacagaag gaacgattac atttgaagca ggagatgagg gtgtaactcc tcctgttgat
    57241 ccagagaatc ctgatcccaa taaacctgtc gatccatcag atcctccaag ccctggaaca
    57301 ggaggggcat tatcgattga ttatggatca aaattcaaat ttgggactca aaaaatttct
    57361 actgcggata aaacgtatta tgcagctgct gatgtgatga acgatgggtc tagaaaaccg
    57421 acatatgtcc aagtgacaga taggagaagt accttatctg ggtggaaatt aagtgtctct
    57481 caaccagaac agtttaaaac tgccagtggg gatgaactag ttggagcaca gctgaaattt
    57541 acgaaagggc aggctgtttc tttggttgat ccaacatata cgccgcaaac ggtgaattct
    57601 gaattaacgt tgacaccggg aggaaacaat acgttagcaa ttaatgctaa atcaggaaca
    57661 ggtgtgggga cttgggtcta tcgatttggt gcaaatgaaa atgaaaatca agatgcggta
    57721 cagctttcgg tacccggtaa atctgtcaag ttagcgcaac aatattccac taaattagtt
    57781 tggacattag aagatacacc taacaactag tttactgaaa agaactgtta aaaataaata
    57841 cgtagtcata ttgtcttgcg aggtaatcga gcaaaaattg tcaaaggcta ggctaaattt
    57901 gtttggatac gaatagttca tctaggaatt taacgatcag gaggaaaaga tatattgaaa
    57961 aagaggagga tcgtgctcat tttaagcagt ctcttagtga ctaatatctt tccgatcata
    58021 gggtactctt tggaattgaa tcagttggag catggaaata gaagagacga agaaagcaaa
    58081 ctcacttcga cattcgacag tttaatagaa tccaaccaga cacaagaaag cagtgaggat
    58141 ttaagtattt cgagtagtgg agagaccact gacaacgtag aagctgaggg ttcggacaaa
    58201 caaggactag aagatgcacc aatttctgcg gttgagctgc ctgacgatcc caatattatt
    58261 tctatcgata aaatttttca aacacctatc ggtactagca cgtccatatt ggaaaatggg
    58321 aagttactgc agttgaaccc agcagaaagg tctcaaagag gagctatttg gtcaaataag
    58381 aagattagtt tactctcgga ttttacattt aaatcttatt tatacttagg aaatcaacga
    58441 gatagagcgg gagatggcat gacctttaca ttgactggcg atcctagaat ggctagtaat
    58501 cccagtcaag ttattggatc gccaggtatg ggaattggct catattctac tcgttctggc
    58561 caaccttaca ttgcgaatgc gctctcgatt gagtttgata cgtataaaaa tagtggttct
    58621 tctgatcgaa tggatcgaga gataactgct gataatagag ggcatggaca tgtcgctttt
    58681 gtcacgccta aaccaaataa taataattat tctggagagc atactggcgt gacggtagct
    58741 cctacatttc tttctaatgg cacatggcgc ttgttaataa ttaaatggga cgcggagcaa
    58801 agaagtctta cttatgattt ggaaggtgtc ggtcgctctt ctcggattat acctgatctc
    58861 attaggcagt ttggttctac agaagtaaat tgggggttta cttcttcaac aggtactttt
    58921 tttcaagaaa atgctgtcgc tgtcacgcga cttccatcga atgtcacatc aacagctgat
    58981 gtaaaagtca acgatagtga ttatggggcc atcgctgaag ctggaggaaa cgataagata
    59041 actatcagaa atacattgaa ggtcgagggg gattttcttg aatataagaa aatgcagact
    59101 agtatcgacc tcccacaaga attaactatt cccgagcata tcttgatcaa tggaaatcaa
    59161 attgatgcca aagatatctt gataaccgga aatcgagtca cgctaaatct gacagattat
    59221 tttattgctg gagatacgtt aataatcgaa ttggagacaa ctttgcagga agtaattcct
    59281 gaaaaagtac tcactatgca ttttgaatac cttgaaaacg ggaatttgat tgagcaatcg
    59341 aatgaaataa acctaaagat cgcaaagctg aaggagatag atatccatgt ttattataaa
    59401 gataaagaca gtcagattga tattgctgaa atgaaaacaa tcactgggaa aataggagag
    59461 aagtataaag aaactcctat tgatattgaa gggtatgttt ttgactcgga ttcagcgaat
    59521 acagaaggca tcttctccga aaatacagag gatatctatt tttattacag aaaaggcaaa
    59581 ctttatctct caactgctcc tcaatatgtc aattttggaa aacataaaat atccagtaga
    59641 ccattaagtg aattcggaca gttttcaggt gggttgaaaa ttatagatga acgggctttt
    59701 ggaagttgga aattacaatt gaaacaagag aaagttctta caaatggaga ttttgagctt
    59761 ccagaagctc tttcctttgt tacctctgaa aatacaacgg ttatagggac atccgctgtt
    59821 acgattttcg aaagtgatca aaagggtgaa agcgatttgt cctctctttt agattctaca
    59881 gctcatcgtg gaatcagaat aaatattcct gtagaaaacc aaagaatcgg aacctttgag
    59941 gggaaactat cttggatact tgcagatgtc ccaggaaatt aacaggaaga gaagaatata
    60001 acaaaattat tgagtttcaa gaagggcaca ggtaccatag gctcttgtgt cccacagaga
    60061 caagattttg ttatagtctt ctcttacata tgattaaagg agcgattttc ttgagaccaa
    60121 aacaacaatt atttataaca ctcctagtta ttttaggggc actttttaac aatatatcag
    60181 tgtcagctag tgcatttaat ttttcagtga cgccgataac ttcagagaat caaatcgaca
    60241 agagaaaaac ttacttcgat cttcaacttg atccggatca ggaagtagaa gtaaaagcgg
    60301 aattgagaaa tgatacagaa aaagaagtga aaatcgatat ttctgtgaat agcgcaacca
    60361 ccaattcaaa tgtgatggtt gaatacggaa aaaacgagat tgaaaaagac atgagcctga
    60421 tctttgatct ggtagattat gtgagctatc cacagatagt gacgctaaaa cctaaaagtg
    60481 tccaaaccgt tgcttttcat gtcaggatgc cgaatgagcg atttgatggt gtattggctg
    60541 gtgggatcac attccaagaa atccagacgg aaaaggacca aacagaaact aaggatcaaa
    60601 gcttatcgat tgagaatgaa tacacctata ttattgcttt gttgatgcaa cagacactga
    60661 acgaagttgc tccgaacctt actcttcatg aagttaaacc agatcaaatc aatgcaagaa
    60721 atgtcatctt agcaaacgtt caaaatgatc aaaaaaccta tatcaatcaa gtagttattg
    60781 aaacaaaaat tacaaaaaaa ggacattctg aagttttata tcaagaggaa aaagaaggat
    60841 tgcagattgc gccgaataca aatttttctt ttccaactgc tttaaatggt caaccgctaa
    60901 ccccaggtga atatcacctt acaatgactg ttttaggaaa tgaaaatgaa aatggaaaat
    60961 tttcgagaaa aaaaggaaat actacgatca actataccaa tcaatgggta tttgaaaaag
    61021 attttacaat agacggaaaa gtagccaagg aattgaacac gacggatgtt acgattaaaa
    61081 aagacaataa ttggatttat atcttagtag cttcactttt cttattgttt attttattat
    61141 gcatactttt gtttgttttg agaaaaaaag aagagaaaga aaagtaaggt taagataaca
    61201 aatagtaaat ttttattatt gtaggatggt tgggctcttt ttataacaat ataaaatata
    61261 ccattttatt aataaaaatg catactagta aatgatataa ttctaataac aggtttgatc
    61321 gcgagaaagg ccaaataaaa cgctctccaa aatgttaaaa aaaagacagg acgatgtata
    61381 ttatacaaac aagaatcgct gacaacagtg actcggatcc aggaaatcag ttttatcccc
    61441 agctagttgg tgaatgcatg gaaagtaata tacgtagaaa gagctgatga ctaatgaatt
    61501 atgagttttt tttaagcaaa gaagatcaca gaagttattc attacttaaa tatttagaag
    61561 caagctcaaa tttatcagag tcaatatcga atgttcaaga agagttgtcg ctatcgacat
    61621 ttctcttgaa aaaaacgatc gataagctga aacatgattt acaaaagttc gatctagaga
    61681 aaaatttgcg agtaaccgtc agtgaattgg atatatgtct ggaaattaat ggaaaatgtt
    61741 ctagcaaagc tctgttatct cgttatataa cggattcttt atctatgaaa atggtattgg
    61801 ctttctttca ggaaaaatat gtctcagtag aaaattttgc taaacagcat cacgtgagtt
    61861 attctgtggc ttataaagta ttacaaagac taaaaagaaa tctaaaaaaa tatcaaattt
    61921 tttttgaaaa gagaaagtt
//
LOCUS       DACDYI010000005        61605 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.005, whole genome shotgun sequence.
ACCESSION   DACDYI010000005 DACDYI010000000
VERSION     DACDYI010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.005"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..2327)
                     /gene="esp"
                     /locus_tag="IWM52_000302"
     CDS             complement(<1..2327)
                     /gene="esp"
                     /locus_tag="IWM52_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAP9972588.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
     gene            complement(2945..3379)
                     /locus_tag="IWM52_000303"
     CDS             complement(2945..3379)
                     /locus_tag="IWM52_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972589.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3396..4601)
                     /locus_tag="IWM52_000304"
     CDS             complement(3396..4601)
                     /locus_tag="IWM52_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAP9972590.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            5295..5681
                     /locus_tag="IWM52_000305"
     CDS             5295..5681
                     /locus_tag="IWM52_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9972591.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            5775..5966
                     /locus_tag="IWM52_000306"
     CDS             5775..5966
                     /locus_tag="IWM52_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9972592.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            6480..6812
                     /locus_tag="IWM52_000307"
     CDS             6480..6812
                     /locus_tag="IWM52_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9972593.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            7108..7263
                     /locus_tag="IWM52_000308"
     CDS             7108..7263
                     /locus_tag="IWM52_000308"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP9972594.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            7539..8879
                     /locus_tag="IWM52_000309"
                     /pseudo
     CDS             7539..8879
                     /locus_tag="IWM52_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9120..10736)
                     /locus_tag="IWM52_000310"
     CDS             complement(9120..10736)
                     /locus_tag="IWM52_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP9972595.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            10854..11072
                     /locus_tag="IWM52_000311"
     CDS             10854..11072
                     /locus_tag="IWM52_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9972596.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            11267..11728
                     /locus_tag="IWM52_000312"
     CDS             11267..11728
                     /locus_tag="IWM52_000312"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9972597.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(11921..12160)
                     /locus_tag="IWM52_000313"
     CDS             complement(11921..12160)
                     /locus_tag="IWM52_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972598.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(12184..13707)
                     /locus_tag="IWM52_000314"
     CDS             complement(12184..13707)
                     /locus_tag="IWM52_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP9972599.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(13725..13847)
                     /locus_tag="IWM52_000315"
     CDS             complement(13725..13847)
                     /locus_tag="IWM52_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP9972600.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(13877..14860)
                     /locus_tag="IWM52_000316"
     CDS             complement(13877..14860)
                     /locus_tag="IWM52_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP9972601.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(14884..15033)
                     /gene="rpmG"
                     /locus_tag="IWM52_000317"
     CDS             complement(14884..15033)
                     /gene="rpmG"
                     /locus_tag="IWM52_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP9972602.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(15054..15431)
                     /locus_tag="IWM52_000318"
     CDS             complement(15054..15431)
                     /locus_tag="IWM52_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972603.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(15463..15642)
                     /gene="rpmF"
                     /locus_tag="IWM52_000319"
     CDS             complement(15463..15642)
                     /gene="rpmF"
                     /locus_tag="IWM52_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP9972604.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(15657..15926)
                     /gene="rpsN"
                     /locus_tag="IWM52_000320"
     CDS             complement(15657..15926)
                     /gene="rpsN"
                     /locus_tag="IWM52_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP9972605.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(16449..18221)
                     /locus_tag="IWM52_000321"
     CDS             complement(16449..18221)
                     /locus_tag="IWM52_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9972606.1"
                     /translation="MRMQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(18175..19929)
                     /locus_tag="IWM52_000322"
     CDS             complement(18175..19929)
                     /locus_tag="IWM52_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9972607.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(20039..20608)
                     /locus_tag="IWM52_000323"
     CDS             complement(20039..20608)
                     /locus_tag="IWM52_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAP9972608.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(20626..22050)
                     /locus_tag="IWM52_000324"
     CDS             complement(20626..22050)
                     /locus_tag="IWM52_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9972609.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            complement(22052..22720)
                     /locus_tag="IWM52_000325"
     CDS             complement(22052..22720)
                     /locus_tag="IWM52_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP9972610.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(22850..23434)
                     /locus_tag="IWM52_000326"
     CDS             complement(22850..23434)
                     /locus_tag="IWM52_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9972611.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(23765..23998)
                     /locus_tag="IWM52_000327"
     CDS             complement(23765..23998)
                     /locus_tag="IWM52_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAP9972612.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(24013..24963)
                     /locus_tag="IWM52_000328"
     CDS             complement(24013..24963)
                     /locus_tag="IWM52_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP9972613.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(24960..25802)
                     /locus_tag="IWM52_000329"
     CDS             complement(24960..25802)
                     /locus_tag="IWM52_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP9972614.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(25804..26523)
                     /locus_tag="IWM52_000330"
     CDS             complement(25804..26523)
                     /locus_tag="IWM52_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP9972615.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            27215..27373
                     /locus_tag="IWM52_000331"
     CDS             27215..27373
                     /locus_tag="IWM52_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9972616.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            27445..28671
                     /locus_tag="IWM52_000332"
     CDS             27445..28671
                     /locus_tag="IWM52_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9972617.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(28759..29151)
                     /gene="rpsI"
                     /locus_tag="IWM52_000333"
     CDS             complement(28759..29151)
                     /gene="rpsI"
                     /locus_tag="IWM52_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP9972618.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(29167..29610)
                     /gene="rplM"
                     /locus_tag="IWM52_000334"
     CDS             complement(29167..29610)
                     /gene="rplM"
                     /locus_tag="IWM52_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAP9972619.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(30129..30596)
                     /locus_tag="IWM52_000335"
     CDS             complement(30129..30596)
                     /locus_tag="IWM52_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP9972620.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(30716..32254)
                     /locus_tag="IWM52_000336"
     CDS             complement(30716..32254)
                     /locus_tag="IWM52_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAP9972621.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            32334..33020
                     /locus_tag="IWM52_000337"
     CDS             32334..33020
                     /locus_tag="IWM52_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAP9972622.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(33221..33748)
                     /gene="msrA"
                     /locus_tag="IWM52_000338"
     CDS             complement(33221..33748)
                     /gene="msrA"
                     /locus_tag="IWM52_000338"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP9972623.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(33832..34509)
                     /gene="pgmB"
                     /locus_tag="IWM52_000339"
     CDS             complement(33832..34509)
                     /gene="pgmB"
                     /locus_tag="IWM52_000339"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP9972624.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(34506..35636)
                     /locus_tag="IWM52_000340"
     CDS             complement(34506..35636)
                     /locus_tag="IWM52_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9972625.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(35636..37819)
                     /locus_tag="IWM52_000341"
     CDS             complement(35636..37819)
                     /locus_tag="IWM52_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP9972626.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(37816..38841)
                     /locus_tag="IWM52_000342"
     CDS             complement(37816..38841)
                     /locus_tag="IWM52_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9972627.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(38886..39713)
                     /locus_tag="IWM52_000343"
     CDS             complement(38886..39713)
                     /locus_tag="IWM52_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP9972628.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(39710..40777)
                     /locus_tag="IWM52_000344"
     CDS             complement(39710..40777)
                     /locus_tag="IWM52_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAP9972629.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(40803..41639)
                     /locus_tag="IWM52_000345"
     CDS             complement(40803..41639)
                     /locus_tag="IWM52_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9972630.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(41636..42505)
                     /locus_tag="IWM52_000346"
     CDS             complement(41636..42505)
                     /locus_tag="IWM52_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9972631.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(42575..43858)
                     /locus_tag="IWM52_000347"
     CDS             complement(42575..43858)
                     /locus_tag="IWM52_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP9972632.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(43872..45557)
                     /locus_tag="IWM52_000348"
     CDS             complement(43872..45557)
                     /locus_tag="IWM52_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAP9972633.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            45832..46857
                     /locus_tag="IWM52_000349"
     CDS             45832..46857
                     /locus_tag="IWM52_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9972634.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(47452..51105)
                     /gene="rpoC"
                     /locus_tag="IWM52_000350"
     CDS             complement(47452..51105)
                     /gene="rpoC"
                     /locus_tag="IWM52_000350"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAP9972635.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(51184..54801)
                     /gene="rpoB"
                     /locus_tag="IWM52_000351"
     CDS             complement(51184..54801)
                     /gene="rpoB"
                     /locus_tag="IWM52_000351"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP9972636.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            55529..56497
                     /locus_tag="IWM52_000352"
     CDS             55529..56497
                     /locus_tag="IWM52_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9972637.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            56515..57420
                     /locus_tag="IWM52_000353"
     CDS             56515..57420
                     /locus_tag="IWM52_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9972638.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            57417..58223
                     /locus_tag="IWM52_000354"
     CDS             57417..58223
                     /locus_tag="IWM52_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9972639.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            58380..59348
                     /locus_tag="IWM52_000355"
     CDS             58380..59348
                     /locus_tag="IWM52_000355"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAP9972640.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(59665..60189)
                     /locus_tag="IWM52_000356"
     CDS             complement(59665..60189)
                     /locus_tag="IWM52_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAP9972641.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            60476..>61605
                     /gene="scm"
                     /locus_tag="IWM52_000357"
     CDS             60476..>61605
                     /gene="scm"
                     /locus_tag="IWM52_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP9972642.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSAS"
BASE COUNT        18691 a        12368 c        10318 g        20228 t
ORIGIN      
        1 cctgcggtga aggttacttt gtggtagcct gcaggaatgt tttctcctgg attttcataa
       61 atatctttac ttacagttac tgctaaatcg gtcgtgccta cagaaccatc ttggtaaaca
      121 attttaatag gaattgttcc agtaatcggc tccgattctc ctacaccttt agctggagtg
      181 aatgtaacaa taccagtgtt tgcatcaaca cttgcattac cgaagtcctt attatagaca
      241 tctgaaattg aaaaggcatc tttagctaaa ggaacatctg gtttatcaaa tgtaggtttt
      301 ccactttgaa ctgtcgtttc tggagttgtt gttataggta cgtatgttgc atcattttcc
      361 atagcttgag aagcttttac agcaaactct acatccacgt cgaaagttcg atttccgatc
      421 ttaacagtta caataccttt cgtactatca gtaacgttag aagtatcagg ctctgttttc
      481 catgaatact ctgcatcttc aggtaagttt ttattattcc gaattgattc tttagcatct
      541 ggtttaaatc caactaccac ggtttgttta tcaaccgctt ttggtgattc cttaataacg
      601 gttgaacctt cttctggttt atcaaaacct ggagaaacga tttggatttt taattcatct
      661 ttcgcgatta atttacttga atctacaccc gtaaattcaa attctacagt atctgaaatt
      721 ggagccccat ctttttcatt tggagcgata gttttttctg ctaatacagt accttcttta
      781 tcaacaagtt gggctttgtg acctgttccc gctaactcgt ggatgaatac tttcccctta
      841 actgttgtgt caacatcata tacttcttct aattcaggtt tcccgatata tggattcgtg
      901 tctccgctct cttctttatc gtcagggttt ccatcaccat caatatcttg caaataataa
      961 taacctgttc cataagtgtt ctggattaat tttttattcg tatccgacaa ccaagaatca
     1021 aagatgaaat cttcaccaaa tggtttattt tcttcaactt gtgctgttgc tttcatatct
     1081 tctaagattt catctaatgg cttagtcaat tgtaaaacat atcgaataac gactggacgt
     1141 ggacttgcat tagcaaaatc gataagttta gttaaatcat tattagttat tgaacctatg
     1201 ccataatttt catgaccttc ccgtcgctcg attgctaaat cagctacttt tgttccggat
     1261 tcaaacgaaa catcatatgt tactttgtca gcctgaacca tgtgcatctg tatacccgct
     1321 acatatggaa ctaatcgttc atcaattttg taattagcat accaaggcgt gccctttgcg
     1381 gaagatgaat atgagaaatt ataatttttc gttaacttat gatctaagat tatagctcca
     1441 ttagcacctg tggatcctga accatctttt gtttgagtat taggtttctc atattttgca
     1501 cttactgatc catgtttaaa ctggtcagga ttattttcag atacatctaa gctattatag
     1561 aaatcatctg aatctgttaa aaagtagccc gtactatcaa tacccggtac aattttatca
     1621 tcgtcttgat gacttaccaa gtagattcga tacattaaac gatcggtctt gagattgtct
     1681 atgcctatct ctttaaaaat ttcatctagg ctcttttcaa acgtaattac cccattagga
     1741 gcggtttgcg tatcggttgt ttctgcccca gcaaataatc catcacttgc acgaatataa
     1801 ttgaccttcc aaatatttgt aggtctaccc agcgaatcat taattcttac aaaggtccgt
     1861 ctagaagatc caattccttt ctttggatcg acctgaattt tttctacata tttagctaat
     1921 cgttcatcta attgaagacg gatttcccag tttttagttg atgctgcaat attatgtttt
     1981 ccgtaaactt caaatccaat tgtattgcct tttagtgtgt ctccagttag ttcttgagga
     2041 ctaaattgta aatccgaaaa gttataattt ttcgtataac caggattctc ctgaaaagta
     2101 ttccccgaag ctactttgtc acctacttgt acggagctta tcgctgtttc aagtgggctg
     2161 cttggagaaa tttcatcttg tgctttaact agttcagttg ttcccaagac aagaccaaca
     2221 ccaatcaaaa cagatgcaac cccaacactt aattttttga tactgtactt caatactcgt
     2281 ttctttgttt tttctagaaa tactctctta ttattcttgc taaccattat tcaaatcaac
     2341 tcccttaata attactttcc tagtaagaat aaaagataaa tctgatacgt attattcaca
     2401 gaataggaat cttactaacc ttatgttaac taatttactt agagaatttc atgaacatta
     2461 caacaaaaaa aagtacattt cttcatgttt ttttcggata tttgtgcttt ttaaccattc
     2521 agcagctgaa aacatataaa aagatatgta tgtattcttt ctgaaattaa gttcatttct
     2581 atgcgatgaa gcatgttttc aaaatattac cctttcttag attaaacttc agagtattca
     2641 aataacggaa taaggagcct tatcccgcat tactgaatga gactgtgaca taagtaaaaa
     2701 ggtgacttca aattccttct tcggaatttg aaatcacctt ctttataatt ataaagtatc
     2761 ggtttcacag catttctgaa tgattcctag aatcttaaac gaatgattgg agttatcaac
     2821 attttttcac tcgtttttag cgaaacaaaa aatgacattt gtcacagcct cattaaatta
     2881 ttgattcttt ttctttataa ttgaattgtc catatttaaa gtgtctagta ctctacatga
     2941 ataattaatt gctttcactc aaatacttat aatctattaa cttgtagttg tttaactttt
     3001 ctgaaaaagt tagacccacg taataatctt cccagctttc tctttcaatt aactgatcca
     3061 atttttgata atccataaat tcatcatgta tcaaaaagaa taaataactt gatattttag
     3121 catctagctt aatcagatta aataatgttt gtttaaaatt gctaccatta atatgactga
     3181 tagattctct agcttcttca cataaacttt ttactttatt aattactact ttgtcatatt
     3241 catttaacac actattatta ccattactta gacgctcaga ttctacgaaa caactcaaat
     3301 cagaaatttc ccaaaaaaat tttttgttaa aatccaattt cctcaactcc tccaaactac
     3361 gtaaatgata tagtctcata ataaaataaa acctctcagc taatgttgtt gaaattgtcc
     3421 actaaaccta attgatattg cttaggagat acacctgcgt actttttgaa tactctcgaa
     3481 aagtgtgaag aatctgaaaa tcctaaacct aaagaaactt cactgatagt atggtttgat
     3541 ttcaacatta ttttagccgt agaaattttt cgcttagaga tataattata aatcgtaata
     3601 tgcatttcat tcttaaagct ttctcttaat ttagtttcac ttacagaaaa ataattagct
     3661 atttccttta cagtaattct tttatagata tttaagccga tgaattgaac gacagaagaa
     3721 attaagggag aaagatgctc tatcttagtt tttccgattt ccttagtata ataaacaatc
     3781 ccagaatccc tcacttttaa gacctcaggt aacgatacag ctaattcgtt ttcttgtatc
     3841 aaagcatctc tactttgata cgcttctatt atatccattc ctaatgttat agacgtttgt
     3901 gctaactttt caaaaacaat gatagaataa tttttttcag accgaatcgt atctccacta
     3961 acacttggaa ttattccatt actcagatta aaaaccatgt tttctaaatt tcgaatatct
     4021 ccatttttaa catattccag tattttattt tcataaagaa acagatatat ttctggatcg
     4081 taattttgtg ataacagatt atctattttg atttgctcga tagttttact tagattttct
     4141 gtatattcaa ttaacggttc atggaataga tcttctattt ttccagtaaa aaaataatga
     4201 actaaaatca gaatatctcg aatgtctcct aaagaataca aagtcaaatt ttgcaatgaa
     4261 ttatagagtc gttctctatc agccattgca acattcaaat tgttcatttt tagtttaaaa
     4321 aaaactttat caatattatt tgctctaaat ggaccaaata agtaatatct ggttttaaac
     4381 atgtatagaa gaaacatctc attaaaatca ccagtaaaga aatggaaatc tttagttttt
     4441 ttaatattat cagttagtaa ctttttataa tcataaaaaa aataagtagt tctatctgag
     4501 cgatactctt ttataatact gaattcggaa ttaaaaacca cgataggtaa ctttaaacaa
     4561 acatgaagag actttaataa gacttcttta tctgaaaaca cttttgtttc tctctttcct
     4621 ttctacatca ttaaatagta ttttatacgt tatctaaaat actattcctc tgtcaaaata
     4681 agctaactta cttcattata aaatatccac ttatcgcttt catgtatatt ccaccgtaaa
     4741 aagtacattt tatcaccgag aactcatcat ttttcatcat gatgaatact atttacatat
     4801 atctagttca aagacaataa ttattatgat ccgttattat ctaagcacta tacagataat
     4861 aggaattttt cgtttctaaa tattccaatt tgggaatcca ataaaaattt tcgttcaaat
     4921 acgtgtctat tcgaactctt ttctagttgt taattatgaa gggggaagaa ataaaaaata
     4981 caatctatac acgaataaga atattatcca aaaggaggtt ttagaaaaga tttattatac
     5041 gtaaaaagct caagacattt tcttagccat aaggacatgt ttgatattta agaagatata
     5101 tgaattttct tttctaagta taaagataac tatccattat caactaaaaa aacatttttg
     5161 aaaataaaca tcagcagtcc tatcaatgca tgtgagctta tagaagttat tcgcagcgca
     5221 gcataattgc attagcttct tttcgtgttt tttgcctctt ttccctttaa atcttaatgg
     5281 aggaggtgtg caatatgtca gataacgacg aacgcgtaaa tgcccagttg attcgattct
     5341 ttgaaaaggt cattttgaac accgctaata cttattataa aaaacaagaa gaaattcaaa
     5401 aacatgaaca acttgatcaa ctcccaccat atttgtttac taaagatact ataaaattta
     5461 aacaaccgat taatgtttta aatgtgactc tccttgttga agatataaaa ttagcaaaaa
     5521 tccttccttt gttgaaagaa cctgaacaaa tttttcttat cgaaaaattt atctttgata
     5581 aaactgataa agaaattgga aaggttctgg ggattacaag acagggtgcg acaaatttaa
     5641 aacatcgact ttacaaaaaa attcaaaaaa tgctggttta atatctgcat aataactaat
     5701 tttgtatgct tctttgctcg ttctcctcct tcccaaagaa atagattata agtccatggg
     5761 aagaaggtag atttatgaat tctttaaaag aagatttcat tttagcaaag gctggaaatg
     5821 aagaagctgt tgaggcaatt ctcaaaagat ttagctcttt gattcataag caatcctgga
     5881 gaactggaaa atatgatcaa gattgttatc aagaatgtat gcttgcaata tatctagcta
     5941 tctcgaaatt cgaaattaaa gaataactca actaagggag tctgtgacat aagtaaaaag
     6001 atagtttcaa atttcgaaca ataaatttga aactatcttc tttataatta taaagtatcg
     6061 attttacagc gttctataat gattcataga atctaaaacg aatgatttta attatcaacg
     6121 ttttttaatt cgtttttagc ttaaaaataa cttttgtcac agtctcactc ctaaaattac
     6181 tttatttacg tatgaagtgg gcaggaggtc gaatccagaa ctgggctatg gtacttcaac
     6241 gaggacttct cctctagaat tgaaaagcat gcgatttacc ttccgtaacg acggtagatt
     6301 tattcaacta tgtgtccaat caaaatttgt ctgatttcaa aaatagtcaa gagaattttg
     6361 gttgttaaaa actcttatct atcaacttcc acagtttagt tgacaaaccc tgatactaag
     6421 ttgggctatg atacactttt cttgactatc agttacgtta ccggaaggaa tgaactctat
     6481 tgagaaaaac atatgatcgt aaatttaaac taaagatttc acaggatatc cttgagaaaa
     6541 aagtaactac caagaaaatt gctgaagaat acaatatctc tcgtccaaca atttcccgat
     6601 gggtttcaga atatcgtcga tacgggaaaa atgcttttgc tggacaagga aaaagattac
     6661 ctgataaagc agatttctac attcttgaac aggaaaataa acgactaaaa gaggaagacg
     6721 agtgttctac gaacacaaag atcgatacgg atctccaggg atcactcaaa aactttacga
     6781 tgaaggaatc gaaatcaata aacgtgttgt agctgttctt atgcgtgaaa tgaatttatg
     6841 cacaagagga tttcatcgac gcaaatcttc tcatggtaga gaaaaaacaa tcaaagaaat
     6901 ggtaaaaaaa tgttgaatcg ataatttgaa cagaaccaaa ttgatgctgt ttgggtgatg
     6961 gatattactt acattccatg taatgacggt cgcctctatt tgtccaccta tattgactta
     7021 gcaactcgta ttctacgtta ctatatggta gattcacaca tgaaaaaaga gattgtcgtc
     7081 cgtccattac aaatttatta aggagaattg cctacggtaa ttcattccga tagaggtagc
     7141 cagcatcggt cttttattta tcaagagctg ttgaacgacc accacatcac acattcagtg
     7201 agccaaactg ggactcctgt tgacaatgca gtaattgaag cgtttcatcg atcaatcaaa
     7261 tgagaattaa tcgactccaa caaatataaa agtaaaatag agatgaaggt gttaatccaa
     7321 gattatttag aagactacta cccaaacaaa agaatccata caaaatttat gatgactcct
     7381 tgacaatttg aagcgaagaa aataagggat ctataatatc tagtgttggt gacgccttct
     7441 gttgccaata ctttttcttt tttctaataa acgcaaacag acaccttaaa atcctttaga
     7501 ataaagtcga cgaaaaccat tcaaaggagt gtttcaagat gtcctattca gagtttacca
     7561 aagagttact agatattcta gacctaaatc ttatctttca tgaagatgcc tttcgcaaag
     7621 agcgaatcaa tgacgaaaca tgttttgttt ttgatggcac attgacctat caaccggagg
     7681 aatgctttca tcgccactat caaaatgaac aaacgatcat taaatagggc tggaaaaaag
     7741 tatccatctt attgaatgat gtcagtaact acaagaccat tctgcggatt aacaaacagc
     7801 gcttcaaatg taaacactgt ggaaagactt ttttagcaga agattcagtt agtgatcgtc
     7861 gctgctccat tgctcgcaga gtcaaacaag ccattcttga gctattaagc gaacctcttt
     7921 ctatgtcttt gatcgcacgt atgaagcaca tttctccgac cactgtcatt cgaatacttc
     7981 gcagccttcg gcctaaaacc atttctttga gccagctgtt accagaagtg gtgtgtttcg
     8041 atgaattcaa atcagtgaaa aatgtctcgg gagccatgag ctttgtcatg atggacggca
     8101 agacccatca actgattgat attgtggaaa atcggcaact caatccttta cgcgactatt
     8161 tcttgcgcta ccctataaaa gtccgtgaac aagtgcgtct agtggtttca gatttctata
     8221 caccctatcg cacattggtg aaagagtgtt ttccgaatgc ccgaatcgtc gccgatcgct
     8281 ttcatattag ccaacacatt ggtcgggctt ttaccaacca ccgaattcaa gtaatgaaga
     8341 cctttaaaaa aggggatcgt cggtcgaagc acttaaagaa atactggaaa ctacttcaaa
     8401 agaatgcgtg ggaattgaaa ggtcagcatc gctactggcg tccgtcattt cgagatcatc
     8461 tgacagaagc agagatagtc gatcggctat tatcttatga tgattcctta aagcgaggct
     8521 acgaagtcta ccaggatttt ctttccgtta tcagaagaca agatgttcct gaattcgtcg
     8581 cgctcctaaa agaagactac aaggagttgc cagagcacta ccaaccagtg ttcacgacct
     8641 tcaagaaata ccaaacagaa atcaaacgag cgttgcgcgt tccctattcc aacggcccta
     8701 tagaatgttt gaataaccat atcaaagtac taaaacgaat tgcctatggc tatagaaact
     8761 ttcaaaacta ccgagaacga atctttttat atcgaggaaa atattttaag aaaactaaga
     8821 atactaccca actgactaaa gccagaacaa caacacggct agacaagata gctgtctagc
     8881 cgtgtgagta tctcgattat tctattgaat ttcttcacca acactcattg acgaagagcc
     8941 aaaataagta atctaaccga ctgttaacta aaccattgca ggtcccgtta aagatacttg
     9001 tgcctgttgt caccgcttct ttttcttgtc cacaagcgct taacacactt aataaagccc
     9061 ctgctattat taataataat atctttttct tattaaaaac ctcctcatct atttccagat
     9121 tagtaaatgt agtaatctgt gctttctttt ttctcaatac taataactaa cttatggggt
     9181 aaacaaatac tcgtttgacc gggttttgag atccatccag ttctaaccgc gatttgatca
     9241 ggactattat cttctttaac tcgaattctt ccatctttaa tctcaacaat attatattgt
     9301 cccttcgcag gatgataatt cacgagttta tgattgatct tatccaaatc gaagcgatcc
     9361 atttccttgc catcaattcg aacgatggca tatttgatag catcttcagt tgtttcattc
     9421 tccttttcta tagagaatac aaataaggga ataaaagagg taataatcaa aatgccaata
     9481 attatgacat cgcctatctt gacaccagct tttcttaaaa atttcattaa cgatatctgg
     9541 ctaaacatat tagacttttt ccatggaaat ggtgaaattt ttcggaaatt atctgcatta
     9601 agagaatcag ttaaacaacc ctcaagttgt tccttaatac attgttgatc catcttcata
     9661 ccaataaaaa ctagtttatt aatgcgttca ccaaacaacg gatgccaggc ttccttcaat
     9721 tcgggatttt tttgtataat catttgacac tcaattttag gtaaactagc tacccaataa
     9781 tttaatgatt caatactttt atagctaccc gatgtttcaa agatcgtcga aaaatcacta
     9841 tgttgggcaa accaaagaaa cccttttgct cttgtaatat tgccaggaaa atttttatcc
     9901 gtaagatggg caaatttttg tgcgtcaagg agtaaccgat ttcgatagat aaaacttgag
     9961 ataccatatt cttctgtttc aggaatatga ttttgatagc caagctctaa ctctttgaac
    10021 cagcccactg aataggaagt ctcatctata ttaaaaagat ttgtatttag aattttcgat
    10081 aagtctactt cacctttaat tgtcctaatt attttggcat tggactgtaa cttagcaatt
    10141 agaccgttaa ttttgtaaat cgtcttggtt gacactaagt cactattatt taaaagtaat
    10201 atgttacaac actcgaattg atcaattaac aagccacgaa tatcctgttc ttcttcaact
    10261 acattacctt cagcttttct ttcctgaatc ttttcacccg tttcgaaatt atcccaaaaa
    10321 cgttttacat ccactactat aataatggca ccaatctcta atcgttgggt caaatcaatg
    10381 tctaaagggt ttttagctaa tataattgct tgaataattg gaattggttt gctgattcct
    10441 gaagcttcaa taacaatttg attcaagtca gaaaattgca ctaattgatc tagttcaaca
    10501 atcaaatctt cgcgtaatgt acaacaaatg catccattcg gcatcgaaac aagtttttct
    10561 ccagttcttt taaaaccttt cttgtcgact aataccccat caacatttac ttcccctatg
    10621 tcatttacaa tgacagcaat ttcctttccg tgatcaccgt acagaatatg atttagaagt
    10681 atcgtttttc ctgaaccaag atagccagtt aagattgtaa taggtatttt tttcataggg
    10741 aacctctttc tataccttga atttacactt taagtgcaac acataaaaat gagtcataat
    10801 cgatatccaa ctagtataag tttgtcgaga acactcctag aaggaaaata attatgagcc
    10861 agtatcgtca tcttactata ctcgaacgag aaagaatctt tctgcttcac gaagaaggat
    10921 tttctattag tataattggc aacgacattc agcatagccc gtttactatt ttacatgaac
    10981 tgagaagaaa caagaaaaaa actattcacc ttccaaagcg gaaaaaaatt atgcttatcg
    11041 aagaaaaaaa tgcggacgtt atcacattct aaatgatccg tgagtttggt caattgttcg
    11101 tcatttattt gtcgattttc agtggtcgcc agaggaaatc tcgaatcgtt tgaagcatga
    11161 acatatggac ttttcaatta gttttatcac gatctaacgt gcaatttgca atgatctttt
    11221 cgatacagaa cccctctcac acggcaataa aggcctgatc cgttcattgc accatcgagg
    11281 aaaaacaaga catacaaaaa atcatgtcga gtctcgtgga aagatttgcc ttactcattc
    11341 gatccatggc cgtccaaaat ccgcagatga tagaagtgat tttggtcatt ggaaagccga
    11401 tattgtagct ggcaaaactg gtggtacttg tttagtgact ttgacagaca gaaaatcatg
    11461 cttcctttta gcaggaaaga aggtccccag aaagaaagcc gcttatgtga gcgagaaaat
    11521 gattgaactt ttaagcagta ttccaaatga ggcggtcaaa acgatcactc cagatagagg
    11581 aaaagaattc tccaatcatt tcgcagttac agaagcattg aataatatgc ccttttattt
    11641 tccagatcct catgcccttg gcaacaatga acaaattaaa atacgaatag actcattcga
    11701 gaatacttgc caaaatcgaa agagatagct acggtagata actcaacgat aaatcagatg
    11761 attgaaaaac tgaataacag acctagaaag tgtttagatt ggagaacacc ttatgaagtc
    11821 ttctttgata aagtgttgcg cttgatttga caattcaaga cataataaaa agcagatcaa
    11881 atccaaataa gggaaaaatg atctactctg gtattatcaa ttaattggcc aagctattat
    11941 gccgttttgc taaacgtcta cttcttccag tattgacctt gataaaacaa caatcaggat
    12001 cccgttttaa tactctatga actgtgtcaa tactaacatg taaagttgca gcaatatcag
    12061 ctaatgtact cccatttaat ctcatttgct ttgcaaatcg ataacgccga taagcatcaa
    12121 tattcttgga atccatagac aataatactt ttcttaccat gtatttcctc caaaaattga
    12181 attttaatga gccatcattt cttgagcaat ttcatgttta gacaatgaag ctggatagaa
    12241 agtaggccaa ttatgcaatt cattatagag tttttcttga ctttctccac caaagaaaat
    12301 atggaaatgc tctgccttac ttggtgcaat tccatgatca ctaaattgaa tatatttaaa
    12361 acgaccagca cctgcatcat cagtttcaaa attaaaacga acaccacggt ttcctttttc
    12421 ataattcaaa attttgtatc caacatattt gtatgtgtat tgatgatgtt caccgttgac
    12481 tacaaaatca atggtgttgt ctttaatgtt gatagtatca atatctgtct tatagccagt
    12541 agtataataa tctttgtact cggcagctgt catgtcttta ttgatttttg ctttgtaatc
    12601 gaaaacttca tccaaaattc catcttttaa caacggataa acagattgcc attcgcctgt
    12661 ataatcactc aattcgcgat ccttcacatc ggcatcttta aagtacccat tagcaactgt
    12721 cttttcatcc ttcgcttctt tttctggaac tacctcttca ccagcaacca tagttgtttt
    12781 ttctaacgct tttagattgt cttccattac tgaaacataa tcttcaccat tatccatttg
    12841 ttcttttgta agactttcaa gaggattcag tacttccaat tttatcccag cttcgttggc
    12901 caaagttttt gcaattttat cattagcatt cttttcaaag taaatataat tgatcttatt
    12961 ttttttaaca tactctttta attcagctaa tcgaccagaa gatggctctt cttcaggact
    13021 tagtccagca ataggaactt gtttcaatcc ataatccaaa gctaaatatc caaaggctgc
    13081 gtgttgcgtc acaaaacttt tttgtttcgc ttctttaaga ctagtggtat agtcagcatc
    13141 caaccgtttc agtttagtca aatacttttc agcatttgtc tcaaatactt ttgctttttt
    13201 cggatacaat ttaactaact gatccttgat atttgaaact tccttaattg ccattttggg
    13261 tgaaacccac gtatgaggat ctagttcatg atggtgatct tcttctccgt gatcatgatc
    13321 ttcttcttca ctacctggca ataaaatcat gtcttttgtt ccttctacta cattaggccc
    13381 agctttcttc caactttctt tagcttttgg tacccatgat tccatgtttt cgttatgata
    13441 tacaaaaaca tcagcatcat gagcttttgc catatcctta gctgagggct catagtcatg
    13501 aggttctgat cccgctggaa ccattaaatc cacgttcgct tcatcgccga cgatgttttt
    13561 ggtgaaatca tacattggat agaatgttgt tacaatttct aattttccat ctttatcatt
    13621 cgatactttt gcatcattag tgctggagca agctcccaat aatcctagag ctgctatccc
    13681 aataagtcct acactgattt tcttcattct ttttccttcc ttaattatgc taatttcttc
    13741 attttatttc gtttatgttc tttgataatt tttaatgccc tttttctagt tttaatattc
    13801 gggcttttaa gtcgtcgata tgcactagat atatccgttt tctccattag aaattacccc
    13861 caatcactac ttatcattat ttgctttccg ccagtaattt atcaaaactc tgtttgatcc
    13921 tttcttcatc caacattttt ccgattagaa taatagtggt ttggtctttt tgtcgagtgg
    13981 tgtattgaaa attaacctgt tcgtttacct cttgcatggc aactataaaa tcctgctctt
    14041 gtaaagcaat caatccttta aaacgataca ggctcccttg gttggttaaa atcagccagt
    14101 caatccattg gagtaaaaaa tcttttttta tgtcttgatc tgaagttaag agaattgatt
    14161 taaactcatg tacttgatgg tgttgatgat agtatttgcc attatagtga ttttctgatg
    14221 aatcactttc aaaaactaag ggatgattaa attttttcaa attataaaaa tccttttttt
    14281 gaatcgtttc actatgtgtg aacattcgaa tatcggataa agggttaata cttttgattt
    14341 ctctctgtaa tttttttagt gcagataaat tcaagttttg ttttctagac acaaaaatgc
    14401 gatctgccat gacaagttgc ttaagggctt gtggttctct ttcaataagt tcaaaactgt
    14461 cactatcaat aacagtcaat aaactatcga tatatactct tcctcttagt tgaggcgctg
    14521 ttaaaagtgt ttgaatgatt ggttggggat ctgctatacc tgtagtttca atgatgattt
    14581 gtgaaattgg ttgttgatgt tcttctattg tttccagtaa agaaatcaat attgctaata
    14641 aatcactgcg aagactgcaa cacatacatc cattatttag ttgtaacacc cgttcctcgc
    14701 tatgaactaa taatttgtga tcaatcccaa cttcaccaaa ctcattttca attataataa
    14761 tttcttccct ggggtagggt gagacttgca aggcttgatt gattagcgtt gtttttccag
    14821 cccctaaaaa cccagaaacg attgtaactg gtatgcccat tttgtcacct ttttaccact
    14881 tagctatttc acttctttaa aaattgcccg tctgcgcaat tttggcgaat acttttttaa
    14941 ttctaaccgt tcagggttat tccgtttatt tttgctggtg agatacagac gttctccagt
    15001 ttcgacacat tccaaaataa tattttgacg cataaatctt cctccttcaa tacttatcga
    15061 tcttcaagat atgcttgaat ttgttgtgca tcttcaaaag ttaactcgcc agattccaat
    15121 tgtgaaacta aattttcttt catatcaaca ggtatctccc aataaccatc gatacattga
    15181 aataactcct cgtcatactc ttcaaaaatt ggatagattc ttaaaaaatc atcaaatgat
    15241 aagccttggt cctcatagta atctttgact aatccatttt caattgcctg ttgggtcggt
    15301 aaacccgttt cagtaataat tcctctctct accatcgaac gataaaaagt gcatttatct
    15361 agcgattgct gggtcgtttc aagataatac tttaatcctt cttcaactaa aggtgggtaa
    15421 ataatttcca ttttgattcc ctcctaaaag ttatatatta aactattttg aagctccttt
    15481 aattactttc tttccattat agtacccttt taaagataca tgatggctac gtctataatc
    15541 accattcgct tcgtcaaatg aaatttctgg ttttactaca gtttgatgag ttcttcttaa
    15601 ccgtttcttc gccttagatg tttttcttgc tggtactgcc atgatatttc ctccttttac
    15661 caacttgctt tttttactcc aggaatcaac ccttgatggg caagttctct aaacttaata
    15721 cgggacatac cgaactttcg catataccct cttggacgac catccgtctg atcacgaagc
    15781 ctcagacgat tcggatttga atctcttggc aatttagcga gagcggtgcg atctcctgcg
    15841 gcttttaatt cctgacgttt atctgcatac ttttcaatca tcgccatttg tttctttgct
    15901 ttagcaattt tcgacttttt tgccatatac ctttatactc cttttatttt gatttagaca
    15961 cctcaaatcg taatcgttac gttttgacac tgatacttta ccaacaagag ttgctcttgt
    16021 caacaataaa taacatgaaa taaaactcat atgaatagat aactatttga aaacgaattt
    16081 atcggtacat agagataatc aaatcattta aaagaatatg cagtttaggc tctgaaaaat
    16141 aagaattcac ttttattagt ccaatcaaac acaaataccg ttttaagtag cttttcctct
    16201 acttaaaacg gcaatatgaa agaaactata actttttatt caatctttta tgtttaactg
    16261 gtttatcgcc aattttttaa aactttgatt attccttata ttataagaat agacattaag
    16321 tgaactaatt caatcatact gattctttcg tgtattcaaa attagatgct gtttaataaa
    16381 attattcttt ttgaaaaaga attataacta tatcccaaaa attgtaggtt aatttaaagc
    16441 ctatttcatc atcgtgattg agcttctttt tcatagacga atttctcaga tagtttagtt
    16501 ttctttatga gatttttgta tatttctgac cttcgataca actcattatg agtaccttga
    16561 tcaactagtt ttccttgatc catcactaca attttattta ccttttcaat agacttcaag
    16621 cgatgcgata taattagtac tgtcttatct tgtattaaac gatttaaact ttcctgaata
    16681 cgtttttcat tttccgcatc taaactggca gtaatttcat ctaaaattaa aattggcgca
    16741 tttttcaaaa aagcccgtgc tatggatagg cgttgtcgct ctcctccaga aagtgaactt
    16801 ccgttttctc cgaccaatgt atgataaccg tcaggtagtt tttcaataaa gtcttcgcag
    16861 ttagctaaac gagctgcttt tttcacttcc tcatctgacg cttgactatt tccaatacgt
    16921 atattttcca aaatacttgt attaaacaaa gttacctctt gaaaaacaat agcgattttg
    16981 ctaaacaaag aagctggaga tacacgttta atatcatacc catcaattaa aatacagcct
    17041 tcatcataat catacagtct agacactaat ttcaagatac tagttttccc ggaaccacta
    17101 gcacctacta acgcagtcac ttctccttgt ttagccgtaa aagagatatg atctaaaatt
    17161 ggggtattgt tatcatatga gaaagagaca tctcgcaact caatgtcaaa agatttcaga
    17221 ggactatctg atccctcttg gatactggtt tccttcatag ctctaattcg ctgtatttta
    17281 ggagctaaat aaaatatttc aagcactgct tctttcatag aatcgaagga atcctttatt
    17341 tttattgcgg ctagtaagta accaacgacg tagagtatag tcacttcacc cgttaacagt
    17401 aagtgaacac ctactagaat gactactgct aaactaacat atgagaaaat agaggataag
    17461 gccatgactg aaaaagtact taattctacc tttaaatgaa tcctttcact ctcttccatt
    17521 tttttgaaaa gtctgtcttg aactttttta gatagattaa agctattaat ctcttgctgc
    17581 aattctatag tttcttgaaa ttcttccgag ttttctctca aagtatcgta atatttagta
    17641 ttggctttcg tttgggattt ctttgataac aagattaaga caaaactaaa taacgttggc
    17701 aaaataacag ctaatcccat tttgacatta ccaaccaaaa gcatcactga aataaaaggg
    17761 aaaaacagag ccataccgcc ggctttaggt attgcatgac tcatcgcatg ctcaatacct
    17821 tcaacgtcag acatgatagt ttgagataaa tctgataaat tatgttttga aaaataggat
    17881 aatggtaaat ttgacaaatc gtctgcaatt tgcactctta aatgcgcact ttctttataa
    17941 gtactattat ataagttttc atactcccta tctaacaacc aatacatgaa aagcaaagta
    18001 acaaacgata cggcaaaata aagccaacgg ggctttgtat tttctaaaac taattcgtct
    18061 atcagcatca ttataatgaa tgctggtacc atattaatgc agtatacaaa aaaactagaa
    18121 atagaagctt tagttaaagc ttctgcgccc tgatcactta aagcaaattt gtttttataa
    18181 aaacttttca ttgtcaagcc tccaatcatt tgcattctca taattagtga ccaattgttt
    18241 atacacttga tgtttagcca ttaattcatt atgatttcca cgttctacta ttttcccttc
    18301 tgacaagaca agaatttcat ctacagattt aatgctggat aatcgatgag ctatcataat
    18361 aactgttttt tctttcatca gatgttgaaa agccttttgt agttcatact cattatctgg
    18421 atcaatagat gcactggctt catccataat aacaatcttt gcatctttta aaattgctct
    18481 ggcaatagct atacgttgtt tttctcctcc tgacagataa actcctttac taccaattaa
    18541 ggtatgctct ctttcgggaa atttttctaa aatcgattcg caaccggcta atcttagcgc
    18601 gtcaaatact ttacttgctg gcgcttgagg atttgctata gaaacgttat cgtaaatact
    18661 ctgattgaat agtttcgtat cttgaaagac aaaagcaatt gtgttaataa ttgtgtcttt
    18721 tgaatagtga tttaaaggct taccaccaat tttaatctgt cccttatcca cattgtagaa
    18781 acctgaaatt agtttagcta tcgtactctt tccgcttcct gattttccta ctaatgcata
    18841 aattcttttt tgttctaaaa ctaagttgaa attttctaaa acacactctt ctttgtatga
    18901 aaagctgaca ttttcaaatg caatgtcaca atttttaatc tgtgtctcgt tcccatattt
    18961 tagttggtct tgcttcattt ctaaataaag ggagtctagt gtttcgactg catatcttgc
    19021 ttcaaacaag tgcatactta catacattat tctcatgaaa gaaacaaaaa taattccact
    19081 taaaaaaaac aacatcaaca agtcaacagc taatatcttg ggagtggtaa gagaagttat
    19141 aaaaaaggtt ataggaacta tcaaaatgca aactatgcca aaaaatagcc attgaaaaag
    19201 aacaaaaggt gttttcccac tcaaagaata cttatatgca aacttagcat aatcgttaat
    19261 tgctttattc aaggctttaa atgatgttac tttagcacca aatatcttta taacttgaat
    19321 tccgcggata tattctaccg tttcagagct taacacatct aacgattctt gatatttttg
    19381 aataaaattt gtattctctg tcattttctt taacaagaat acaccaatta ctaacatggc
    19441 gattagaacc aatccaattt taaagttaat gaaaaagcct aaggcaactg ataaaacagg
    19501 tgttactatg gcttgagcac tatctggaat caagtgagca attgccatat gtgttttagc
    19561 cgcgttgtca tcaattgtct ttcgaatata tcccgaagaa tttaaatcaa aaaaacgaaa
    19621 acttgcttct gttaaaccgc taattccctt ttttctcaaa ttagtctcta agcgaaaccc
    19681 aaggatatga gaaaataagc cagaaaaaat ataaaataaa gctcctaaag ttaaccaagc
    19741 aacagtttga accgcataac tcttagctaa atattcatct cctacaataa ttagagactg
    19801 aagaaatttg taaatactgt aatatccata gactgttaat acgacagaaa aatcagaaaa
    19861 taaagtggct aaccatccgt agtatttcat tttagggaca taactaaata attttttgta
    19921 tactttcatt ttttcctccc ccgcattcaa tttcttctct tattcgttta caactgttaa
    19981 tgctttccgt aatagcgtaa attagagatg tacaaacaga taaaaggtaa actacgttct
    20041 attttttgta tttttttaag acttttcttc caatcaatac accaatcata gataaacttg
    20101 ctacaactaa cactgaaatg attccccata tacctaccgc atagtggatc attgcatcca
    20161 ttgttgcagc agattgtcct ttatctatcc aatcttgacg aaaacgatct gtaaagaacc
    20221 atataggaat ccactgtccg gcagcactgc ctaattgcat tagagcccaa gacgcaactt
    20281 gaggaattgc tcgatcatat ccaaatacag atagaataat atcagctaag atcgccatac
    20341 taatcgaact gataaaccaa ggtaagtacg ccccgcccat taacgtaaaa aataacaata
    20401 aaacaattgt gtaaatagta aaaattcctt ttctagggta gttatagcta atataaacaa
    20461 atatgattcc agataataat ccaaaaatcc cgggggatat gctgtgacca aacgccccac
    20521 caatactact aaccattatt gaaaaaaagt aaattactag ggaaataact gtagctaaac
    20581 caataaaaat ataatctttt gttttcatat atattctcct caattctatt tcgttatgtc
    20641 attaaaaatc tgttctatca aagttgaatc ttctactcta agttttatat ctttttttat
    20701 tccaccattt tttaaataaa taattctatt gcatgtttta taaagaaatt ccggatcatg
    20761 agtagctata actactgcac ccttttcttt taagtcttta ataaggtcag caatcctttc
    20821 catattaata taatccaacc cacttgtagg ttcatcaaat agaaataaat tagtttttga
    20881 caatgaagct atagaaacca ataatctttg tttttctcca ccggataaat caaacggtaa
    20941 ttgatctttc attttataaa gttccatttt ttttaaaatt ttttcttgct gatctttact
    21001 aactatttca ttccccaggg ctaactcgct cctcactgat tctgtgaaaa attggtaatc
    21061 catttcttgc attactaaaa acggcgatga caaatttttt ttattttcga ttatctttcc
    21121 tttggtaggg cgaataatct tcatgagtat tcttagtaag gttgttttcc ccacaccgtt
    21181 tttccctacc aaaccaataa catctccaac tttgacatca aaatttatgt cttttagaat
    21241 attttcataa gtaatttctt caacttttaa tacatctgca cttacctttt cgatataggg
    21301 aacttcaatc gaactattaa aaatgtcaaa tttccttaat gcgttattgt tttcagtttt
    21361 taacaactga atagttgtag tttttacagt tctattttta acaaatagaa cttgatcaat
    21421 taattctttc aaatagtaaa aacgatgttc agctataaca atagtaaatc ctttattttt
    21481 taaatcacaa agtaagtcag caagtttctt agtcgctttg aagtccaaat ttgaagacgg
    21541 ttcatccaat ataattaatt tagagtttag cgataaacta catccaatag aaattttctg
    21601 ccgttcacca cttgatagcg aaaaaatgtt tttatctaat atatttgaaa gatctaataa
    21661 ttcagctaat tcattgattc ttttatccat atgctctttt gtaaatccaa agttttccat
    21721 cgaaaatact aactcactcg ttgtatttgt tgtaaagaat tgtcctctag gatcttgact
    21781 tacgtagcca atattttttg atatactttc ttgtgttaag gattgtagcg agttattttc
    21841 aaataaaatt tccccaacca attttccgtc ataaagtctt ggaataagtc cattcattaa
    21901 ctttaatagt gtggatttcc cactcccaga agaaccagta atcaatgtaa gactgccttt
    21961 ttttattgaa aaattaaggt caacaagcgc ttgttcctta acatccttat atgaaaaaga
    22021 aacattgtta aattcaaaca attctttcac ctcaaatcaa aatacttaag aaaaaaatag
    22081 taacggaaaa caggatgata tagtctaata agctcatgtt tgttttatag taagaacttc
    22141 ttttcacagg agattctatc ccctttgtta ttgcattagc ggtcatttct tcagcaatca
    22201 tagttgcacg tataagaacg ggaactaaca gaaattccaa atattgcaga ggctgaaaaa
    22261 aagacaagcg aatgtttctc atttttaaag attctttaat cattcttatt tcagaacgat
    22321 aagtcggaaa gaatctgatc actactgtaa tacttaacat tatttttttc ggtaaatgta
    22381 ttttttctaa agaggataat aattcattgc tagtatagac actaaccaac aaccatgaga
    22441 tcattacgac cggcaacatt cgaaatacaa tatataagga tgtcgcaaaa aatatagtaa
    22501 aaaactctat cttgatagca tcaataattt tcattactaa aaacagaact gaataaatca
    22561 agatgaaata aatacatgtc tttattttac gaaacatcaa tactaaaact gtagcaataa
    22621 tgaaacaccc cacttgaaat atatttccct ttcctaaaaa aataatgacc ggaaaaaaaa
    22681 tatttaaagt aatcaaggtt aacggattga tttttgacac tctttcacct ccaaaaaata
    22741 agatcgcact gttatcttaa gctgataaaa atgcaccgtt attcagataa cagtgcgatc
    22801 ttatcaaaaa gcataactga gaacaattct caatgcaagc tttttttaat taactcagta
    22861 attcaatcca cccaggaaca gaaaattgat acaatcttct ggttaatagc atagtctttt
    22921 cttttgaata gctatgcttc aatatatcat agaatgtagc gtagaatgag taaatgtaga
    22981 agtggatttc ttcttcatta aagactaaat tttgattgat cattccgata gtctttaact
    23041 tatctagaca acagactgtc tttttcgtca caaatgatgt aattttttca acaatatctt
    23101 gatatttggt tccagctgat tggaataaaa tcaaatcgaa tatacgttta ttctcaaaaa
    23161 tataattaat aaatggatct aattcattga agttctctct ccaacttttc tctatatcta
    23221 tattttctaa gtttaagagg tatttggtaa actcttcatt atacatatta ataagcgcat
    23281 taatatgcgg ctgaataatt tcttcaaata acgcttcctt agaagtaaaa tgtttataaa
    23341 aagcacccgt agttacgcct gcggtcaggc acagttgcct tatcttagtt tgctgaaatc
    23401 cttctttttc aaaatggctt agtccacttt ctattaactt ttggtgagtt ataatataat
    23461 ctctggtttc tatgggtact tcctcctaat aagatgaatt aactttatta tcttacacag
    23521 gacttgaagt ttcaatcgtt aacgtttatt actgaactca ttccttagag ctaaactaat
    23581 gagaaagagc agaataactg atacaaaaag caatcctaca agaattaata gactcatcta
    23641 aatcattcac cttttctaaa ataatccgag aaagatttaa aaaaattata tccagctatt
    23701 aggataagtc ctatcataat cactccaata gccattttct tatcctccct aaaaataaag
    23761 tcttttactt aaaacctttt ttgtaatcgt caataaattt tttcccttta tcagttaatt
    23821 gaaatcctat atctaaatag tcctcatatc taagtttagc cagcatctca cttacagatg
    23881 ctggactaac acttagaact tttgctaaat cattattttt taatttcttt tttctttttg
    23941 ataattcctg aattgctagt aaataaatta gtatagtagt cgttttaatt tgtaccatac
    24001 ctaccctcca aattatttcg aaaggccttc agcaatttta tccaggttcc atttcatcat
    24061 cgcatagtaa ctatcaccat cttgtccttt ttctgcaatt gaatcagtaa aaatcgttga
    24121 atagatagga atattggtat cttttgatac tgttttcatt ggtctatcat ccacactgct
    24181 ttctacgaat aaggagggaa cttttgttgt gcgtaatttt tcaactaagt gttttatttg
    24241 atctggtgtt ccttcttctt cagtgttgat ttcccaaatg taagcagaag gcacattata
    24301 cgctttcgag aaatatttaa agcatccttc acttgtaaca atcatttttt tatcattcgg
    24361 aattgaagca aatttagatt tagcttcttt gtctagagaa tccaattttt caatatactt
    24421 atctagattt tctttataga attttttatt atcaggatct ttttccgcta actgtttttc
    24481 aatattttta gcgtaaataa taccgttttc taaatttaac caagcatgag gatcttcctt
    24541 ccctttctca ctctgaccct ctaagtaaat aacatctatg ccatcacttg ctgcaaaata
    24601 gtctttgttt tcctctttgt tcgcattttt aactaatttt gtaaaccaag catttcctcc
    24661 agtttccaag ttaacaccgt tataaaaaat caaatctgct tttgaagttt tttgaacatc
    24721 ttcaggcaaa ggttcatatt cgtggggatc tttcccaata ggtacgatac tgtgtaaatc
    24781 aattttatct tttgctatat tttcggtaat atctgctaaa atcgaattag taactactac
    24841 ttttaatttt tcgttcgaag ccactttatc tttgtcttta ctatttattg aacatgcccc
    24901 gaatataaaa agagcaatca aaatccccag acttcccagt actgctttcc attttctcat
    24961 cataacgatc tctccttctt tcttaaaaat ttttgttttg gtgagattag aaaactaatt
    25021 acaaataaga atgccgctgt taaaacaata cttgatcctg ctgctatgtt aaaactataa
    25081 ccaataaaca atccaagcaa tgatgaactt gctccgactg ttgcagaaat aaatatcata
    25141 gttttcaagc tatttgcata taaatatgct gtagctgctg gtgtaattaa cattgcaaca
    25201 attaatatag ttcccacact ttgcatagct gttactgcta ctaaagttaa tagaaccatc
    25261 aatagataat gataaaaact gacattcatc cctattgctt ttgccattaa tggatcaaaa
    25321 gaagtgatta ataattcttt aaaaaataga actactataa ccaacactaa aaccgaaacg
    25381 ccaattgtaa tccacttatc aatatcttgt acagctaaaa tatttccaaa taaaatatga
    25441 aataaatccg ttgagctatt tgcaactcct ataaggataa ctcctagtgc taaaaaagaa
    25501 ctgaaagtaa taccaattgc tgtatctcct tttattacac tattgctttt tataaaagta
    25561 ataatgatag aagctaatag accaaataca atcgcaccaa taaaaaaatt aatacctaaa
    25621 atataagaaa ttgctactcc tggcaaaaca gcatgagaaa tagcatcacc cataagagac
    25681 attcctctta aaataataaa acaacctact gttccagaaa caatccctat aactacagat
    25741 gtaattagag cattctgtaa aaagtgaaag tcatacagac cattaataaa attttgaagc
    25801 atattattcc ccctctccaa taaatatagt atctccatag gcttttttta aattttcttc
    25861 attaaataca gattctacac ttccgtgagc aattagattt cgattcaaaa gaataatatt
    25921 atcaaaatac ttctttacct tgcttaaatc atgatgcaca attagcactg tttttccctg
    25981 atgtttcaat ttttttaacg tatccataat tattcgttca ctaactaagt ctatccctac
    26041 aaatggttca tctaaaaata tgaaatccgc attttgagca agacatcttg ctaaaagtac
    26101 acgttgaaat tgtcctccag ataattctcc tatttgatga ttagaatatt ttcccatatc
    26161 gaccttttcc aaagcctttg aaactctttg ccattctgca tttttaattc tttgaaaaac
    26221 tttcattccg gcatatgttc ccatagatac acattctttt acttttattg gaaaagtaaa
    26281 atcaatatca gctttttgct ctacatatgc aatcttcacc agttttttat tgatatcttc
    26341 attattaatg gagacctctc cctgatgagg gataatattt aacattgctt taagcaatgt
    26401 ggattttcca gcaccatttg gaccaataat acctgttaaa gcaccttctt gtatttttaa
    26461 agaaatctcg ttcaatgcta aaaaatcgtt ataacaaaca ttaagctttt tcacttctaa
    26521 cataactttc tccttctaaa tgataatgat tatcacgatc atatggtata caaaaattat
    26581 attttagtca accctaaatt ttatagttta gatttttttc tgttgctgaa gattagaatg
    26641 acaaaactat ttgaaactac gcgtatagta agtagctttc aaaatatata aaaaaggagc
    26701 gttctcaaat gagaacatgc tcctaaaact actttattgg cgtatggaaa aattatactt
    26761 gaagtggata gcgggttgaa taagttgata agcatcaaaa catttataaa aattattcaa
    26821 gactatgtct gttccatata caaatatgaa aatcagtaaa ctccatttaa cttttttcgt
    26881 agtgtgtctt tttatacatg tttatcgtta ttaatagtga ataggaaaaa aactattttt
    26941 gatcattgac aattaaatac aatttttgaa taacgatccc tgctattcga gctagaaact
    27001 caagtacgcc atgacctgat tagcgacaaa tacttagaga tttctttttg ttctgggaaa
    27061 acaaaatgcc atgacaatag cagcaaataa tgatacttcc gtaaggctcg ccgtaaactg
    27121 gggagaggtg aaattcctat gtgcttaccc aaagcaattc aaaaatttca taactactat
    27181 tttaggagat gattttatag aatccagagt ctacctgaat aaaaaacaaa cctgtgcatt
    27241 tctaggaatc gataataata ctctagataa gtggattcgt atatataatt tcccagtaat
    27301 aagagttggt ggtgtagttc gctttgacgt agaagatatt aaagacttta tgaatacatt
    27361 taaaataact tgaaattttc ttctcgccgc atatactaaa attagtgaaa tatactgcct
    27421 tacttttttt aaggagtgag gattttgcct acaaagcttt caaatgggaa atataaaact
    27481 aatctgagat atccaaaaag gtttaaagaa ataacaggaa ttgcatcaga aaagtatcaa
    27541 aaaacatttc caaataggca attggcgatt aaagccgaaa atgatatgaa gaagaaaata
    27601 gaaaaagttt tgagagagga gaatgcaaac tcattagaat taaaaggaaa aataacgttt
    27661 aaaaaatttt acgaatctaa atggcttcct agatatgaat taggtcaaac aattagatca
    27721 aacaggcctc ctagtgatat aaccattagt aatacaaaag atatatttcg gttgcatatt
    27781 ttacctatgt ttggtgaata cgcaatgaat tatctaaata tcaatactga gataatatca
    27841 gatgaattaa ccaaaaaatc gaaagaatat gcaaatataa aaatcataaa aggatatgtt
    27901 cgttccatgt tcgatattgc agaaatacta aattatatcg aatttaatcg aactactaaa
    27961 attattcaaa gtatcactgc acctaaaaaa aatgctttag aagaaaaaag aatacaagaa
    28021 ggtaagcaag cactatcatc aaaagaactt actaattgga tagaagcagt aaatgatgat
    28081 tttaataatc atttacttac ttttcatgat tacacacttt ttatgattac cctgtatctt
    28141 ggtgatagaa aaagtgagac ttacgctttg caatggaaat atattgattt tgaaaaacaa
    28201 acggtccgtc ttaaacatac tttagataaa tatcaaagaa aaaagtttac aaaaggtcga
    28261 aaagataccg tcattcaagt accagaagtt gtaatgacat tgctaagtga atggaaatcg
    28321 gtacaagctg atcaattgtt gaaattaaaa attaagcaaa cgttagatca gtatctcttt
    28381 acttatacta aaccatctgg agaagtaaat tgtcctgttc atgccgatta cctaaactat
    28441 cgaatcaatt caattaaaag aaggcatcct gacttagttc atttatctcc acacaaactc
    28501 cgtcatactt atgcaactat agctagacaa ggtggagcag atatgaatca aatttcaaat
    28561 gctttaacac actcagatat ttctacaaca aagatatatg tgaacacacc agatatagtt
    28621 gataaggctg tttttgaagc ttttcagaga ggattgaata agtgtgattg atatgtgttc
    28681 ttaaatgtgt tcttaaaaac aaaagaagca ctttccaaat aattgaaaag tgccagaaac
    28741 gttgatatat aaaggatatt aacgttttga gaattgtgaa gctttacgtg ctttcttaag
    28801 acctggtttt ttacgttcga ccatacgtgc gtcacgagta agtaagccag cacgtttaag
    28861 agctaaacgg aagtcaggat caacttctag caatgcacga gcgataccat gacggatagc
    28921 tcctgattgt ccagcgtatc ctccgccgtt tacgttaaca aatacgtcat atgcgccttt
    28981 tgtttcagta acagcgaaag gttgattgat tacttcacgc aagtcagcat gtggaatgta
    29041 ttcttcaaca tcttttttat ttacagtaat tttgccagtt cctggtacta gacgtacgcg
    29101 agcaactgcg tttttacgac ggcctgtgcc gatatattga acttgtgcca atgaaattcc
    29161 ctcctattag attaagtttg tgatgtctaa aacttctggt tgttgtgctg catgtggatg
    29221 ttctgctcca ccgtatacgt tcaattttgt aaattgtttg cggcctaaag tgtttttagg
    29281 aagcatacct tttacagaag tttcgatcaa acgacgagaa tttttagcac gcaattcgcc
    29341 agcagtgatt gatttcaatc ctcctgggta gttgctgtga cggtaataaa ttttgtcagt
    29401 cgctttttta cctgttaatt tcactttgtc tgcattgatg acgatgacga aatcgccagt
    29461 atccaaatga ggtgtgaaag ttggtttatt tttaccacgt agtacagatg caacaactgt
    29521 tgaaagacgt cccaatggaa catcagttgc gtctactaca taccatttac gttctacttc
    29581 gcctggtttg gccatatatg ttgtacgcac gttattttcc tccaatttaa gttctgatgt
    29641 ttgacataca cttcgagttt ccggggctct ttgtggggca aacaatacca tttaacatat
    29701 tacccgctaa tctagtcaat gtcaacgttt ttctgcttga ttgaaaaatt tatatgaaac
    29761 tttacttctt ttctttcttt tatataaaag ggtcgtttcc tgtcaaaatg aaaaacaaaa
    29821 agaggctgtg acaaaagtct tgtcacaacc ccttattttg aataaacggt gttctcctgc
    29881 caactcctcg gtcacaagtc acaatatcca taaaacataa gaagctgttt tttaaaattg
    29941 tttcttgtga ctcggagctg aacggcaggc ttacaacctc ttggtaaacg gtgttcaatc
    30001 agctgaccct cggtattagc tcaaaaaacg gcaaaatatg aggaactatt ttgacgtttg
    30061 tttctctaat actcaggtca aaacgctgat ttcacaacct ctttttcggt caagttcaac
    30121 aacagttttt acgcgtcgat ctctggcgct ttgcccaaat gagcttggat catccagata
    30181 cgtttttctg tggctgtttt gaattcgata aagatatctt gtgttggatc gtcgccttct
    30241 tctccagaaa cttcgattcc tttttggtaa agatctgcta gataacgata gcctgcaaca
    30301 agtgtttcca aacgttctgg aatagtacga tcccagcggc caatttcatc agggattttt
    30361 gtgttatctg caaattcacg caatgtagaa taaggtgaac cgtctaatgt gatcaagcgt
    30421 tcggatactt catctaactg agcgttcaaa tcatccatga actcatccat cattgggtgc
    30481 aatgtaagga aacctggtcc tctcatgtac caatgtgttt ggtgaacaac aacagacatt
    30541 tggcttagat ctgcaactaa ttgatttaat acttctttgg tttgttggaa tttcatcttt
    30601 cttgcctcct aacattttct tacacttaaa ataatagtag actttcaatg aaaagtacaa
    30661 caaaatcatt tacctgagaa cgattttcag ttcgcttttt tatccgtaaa gccaatcatt
    30721 tttcagaatc agctaattct tctcgcagtt gagcaaattg ctgataagtc tgtgctaaat
    30781 gcttttgtgt cgaaacaaat aatggataga ttttctgata ttttttatga tcagtttctt
    30841 gtactccaac ttgctctttc aattctatca attcctgtgc ttcactgata ctttggatct
    30901 ttccgatgct tctccagcct aaaatcacag ctcctaatgc ggatgcttct gtgacagtag
    30961 ggaaaacaag cgggtaaccc agtacatcag ccatcatctg acgaaaaatc ggagattgag
    31021 caaacccgcc tgtagcacgg atctctgtga gctgattttc ctggcaaact ccttttaaaa
    31081 tccgataaag gttgagacag atcccttcta gaatcgcctt catcatttct ttttctgtat
    31141 gttgccgctg taaaccgata aaactgccaa acgcatctgc gtcccaaagc ggtgctcgct
    31201 caccaagtaa atagggatga aaaatcagac cattggcacc cgctggcaca aaagcgatag
    31261 ccgaaagcag tgcatcatat ggattttctc ctttttcttt agcagattga acgacttcct
    31321 gtaaaaatgt atgccctgcc caatcaaaga tgccagcacc attgctagtc gctcctccca
    31381 ctacccaatg tttctgatcc agaacataac aaaacgtctc catttctgga tggaactgag
    31441 gctgagtagt aataaaacga agtgctccgc tcgttcctac ggtcaaagcg gcgatcgtat
    31501 caccaaaagc acctaaccct agattagaaa gcggcccgtc tgcccctccc aaaacaaatg
    31561 atatatcatt tggcaaacct agttctgctg ctgtttttga tggtagacct gttacttgat
    31621 aggtggaagg aaagacttgc ggcaattgct ctattcgaat gttcaagtag tcaagcactt
    31681 cttctgccca ttggaattca tggatatcaa aatatccagt tcctgaagct gttccgtagt
    31741 cgctgatcag ttcgccaaac aatctataaa agatatagtc tttgataccg acaaaataag
    31801 cagttttttt gaataattca ggttggctgt tttgcagcca cttgattttg ttcaatggtg
    31861 tcattggatg aatcggcgtc ccagtctgcc gataaaattc tttgccaagc acggtatatt
    31921 tcagtttttc tgcttcgtcc gctgctcggt tatctgccca agtgatcatt cgagataacg
    31981 gtgttttctc ttcatccaac aatatcaggc catgcatcgc agaggagaag gagacaaatt
    32041 gaatgattgt gtctgaaggc aactgtttaa ttacttcttt tataacggtg cagacacctt
    32101 gaaagatcaa ttcctgatct tgttctgcca tcccttgtgt ttcttggatc aaagggtagc
    32161 gaaaataagc ggatgctgct tcttgggctt catctgtgaa agcaactgct ttggtttgtg
    32221 tggttccgat atctaatccg attgaaacgt tcattttttc acctcaaatt ttatgtatgt
    32281 acaaaaattt agtttaagct tgcgtatctt ggttttgaaa agggggagat ggcgtgttac
    32341 tttattttat cattggttgc tgtatcggat catttttatg tttaaccgcc cagcggattc
    32401 cgttaggtca ctctattatt tatcctagat ctcattgtgt caaatgctgt cgttcgcttt
    32461 cttggtatga attgattccg attctctcga tcatcgtcca acgatttcgc tgccgctatt
    32521 gcaggtgtcg gttgcccttc tactatttgt tggcagaagc cttatgtggt ggtctttttg
    32581 cctggttctt tactttttct tccgcaagaa atttttcgac gttcatatgg atgctttcgg
    32641 ctttgctatt ttcgttgatg gatttatttt acttcatggt ccattctcac acgctattct
    32701 gttcttgggc aattttatgg attttttggt tgcaggccgg cgtcttccaa tggcaaagtg
    32761 tgctgttaac gtttatcgta ggcggtgtgt gccttcgcta tggtcaatcc tatctcggag
    32821 ctggcgatac tctgttgatg ctttcttgga gtggtggctt gtctctagaa gagttgcttc
    32881 agatcttgtt tcttgctagt ctgtttggaa tatccgtttt ttctgtttac tttatctgtc
    32941 ataaaaaaaa gttggaaaag ctgcctttca ttcctttttt gagcagtgcg ttgctgattg
    33001 tccttcatct taggtattga aaaaaggttg tgacaaacga cttgtcacaa cctctgatat
    33061 atagtgttct cttaccaaat ccttgtgact tggagctagg cgacaggttc acaacctctc
    33121 cactaaatgg tgttcaatca gctgaccctc agtattagct caaaatgctg gttttacaac
    33181 ctctgatata cggtgttcca taagtagctt tttcggcttc ttatttcttg acgccccaat
    33241 gtcgacgaag gaaacctgca cgtccagaac cttctcgata aagattgtaa tggagcttgt
    33301 ttttcttgta ataatcctga tggtaatctt ctgcaggata aaattcgctt cttggttcga
    33361 tttttgtcac gattggctta tcgaatcgcc cgctgtcttg caatgcttgt tttgaacgct
    33421 ctgcaatatt tcgttcttct tcatcattat agtagatcac tggacgataa gaatcaccgc
    33481 gatcagcgaa ctgtcctaat gcgtcagtcg gatcggtttg ctgccaatag atttccacca
    33541 actgatcata agaaataact tctggatcat attcgatctc tactgcttct gtatgccctg
    33601 ttgtgcctgt tgttacttgt tcataagttg gatttggtac atgcccgcca gtataaccgg
    33661 atgtcacaga aatgatccct ggctgctctt caaatggttt gaccatacac caaaagcatc
    33721 cgcctgcgaa aactgctttt tttgtcatat aaaatccccc ttttttctca tcataccttg
    33781 atttccaaaa ggaggataac gttttgcttg tgacagaaaa aacggaaact attatatacg
    33841 tgatacggct tctgataaat gccaaaacga acgtacttga tcaaaaacgg ttaaatcagg
    33901 aatcacatat tcagcagaag gaagatttat gttttcacct attcccaata catgcatgtt
    33961 accagccaaa gctgcttgac aaccagcttc tgaatcttca atgaccaagc atgcatttgg
    34021 cggtacattc aattgctgtg ctcctttgag aaaaacttct ggatcaggtt ttgcttttga
    34081 tacttgcgtg ccgtcaataa ggacatcaaa ataacttgtc aaattcgttt tttctaaaat
    34141 gaggcgtgca tttttgcttg ctgatcccaa tccgatcttg atgtttgttt ttttcaagta
    34201 ctcaagaaca gctattgcac caggcaaaac ggatgtttca tccatttttt gaatcgcttg
    34261 aagatataga ttgttttttt gctcagcaaa ggctgatttc tctttttctg agaatttctg
    34321 ttccattttt ccaaatgaca gcaatcgctc tagtgactcg gttctggata ttcctttcag
    34381 ttgctcgttt tctttctcag tgaatggaat ggacagctgg ttagctaaaa ccagccacgc
    34441 ttcgtaatga taacgagctg tgtctacaag aacgccatct aaatcgaata gtgctgcttt
    34501 gattttcatc gattacctcc ttgaaaatga acgacttgaa gtaaagatgc gatgttttta
    34561 gctgtgcgat gaagattatc agtggcttgc tgtaaagctt cttgtaaagg cacagctgca
    34621 ggaaggatcg gaaaaacggc cgaaatgcca acatctgctg ctttttcgct tcctttacca
    34681 acacttccgc aaatagctat cacaggaacg ccctctggta ctcttctagc aatcccaact
    34741 ggcgcttttc cttttagact ttgtccatct agacgtcctt cgcctacgat tacgaggtct
    34801 gcttgctgta cccgtttatc gaactccagt aaatccaata cgtagtcgat gccagagact
    34861 attttccctg aagcaaatgc taacagtcct gcacccattc caccaccagc actactgccc
    34921 gatgcctcga ttacttgagg ggcaaatctc atataaaaat catgcatggc ttggtcagcc
    34981 actgctaatt cattttcttt caagcctttt tgtggtccga aaacaaaaga agcgccttct
    35041 tttccgcata aaagattagt aacatcagta atgacagtga ttttggcttt aatcagctca
    35101 ataggcacgt tcgttttatc aactgtagca attttaccta gattttctcc cagtgcttct
    35161 actttttgat tatcttgatc gtaaaaagca taacctagac cttctgccat gccgatgccg
    35221 ccatcgttgg tactacttcc gccaacgccg atgatgatgt gtcgaacacc ttgttgcaac
    35281 aattgtcgta tcagttctcc tactcctcgc gtggataacc taagtggctg ttgttggttt
    35341 agtgggacat ctttcaatcc gacgatttct gccatttcaa aaatcgcagt ttgaccgtct
    35401 gaagcgtact ggatttcttt gtttttgcca aatgcattag taacgactgc tgtttgccgc
    35461 ttcaacctta aactgtctgc taatgatgaa accgttcctt ctcctccatc gcctataggt
    35521 agcagatcat attcagcctg aacaaaaact tcttgaaaac ctgattgaat ggcttgtgct
    35581 gcctctaagg ctgtcagact ttctttgaaa gaatctggcg caatcagaat acgcattatt
    35641 ttcccctccg ttccaagatc agttcttttt ccagctggta ttggtctcca ttgacaaaaa
    35701 taggtaatgt ttctttcgtc tcttttttta ttactatttg tttttttgtc aaaacaaata
    35761 gcaacctgtg atttttccaa ataaaaggaa aagaaatttt ttcccatgct gaaggaagtc
    35821 ttggagaaat agaaagagtt ccattcgtta ctgtaatacc tgaaaaacca aatataactg
    35881 ctagccatag tgctcctaat gaagcagcat gaattccgtc atctgatgaa tgcggatctg
    35941 gtcctaaatc aatcaggcac gcttcttgga ataaatgata cgcccattcc atttcttgga
    36001 tacggcaagc ctctatggcg tgaatcgctt tactcaacga tgagtcgtgg attgttttgt
    36061 tttcatagta atgcaaattc ttagaaacga ttggttgaga aaaatactca ggtaataagt
    36121 ataacagcat gacaagatct gcttgcttgc tgacttggag ttcatttact tcgctacggg
    36181 cataatcaag taaaatagac tggctgccag gacgttcttt gtaagtggtc aaatcaataa
    36241 ctggcttact gagatacgtg tcatcttgcg gaatcagctg ttctttgtta ggcactggca
    36301 aatataactt atctaaaaaa ttttgacatt ctttcaaaaa aagcggatcg ccagcttgat
    36361 atttaacagc taaggccaca ttataatgcg ccagatagtt cgtatatgca ttattgtcga
    36421 tatattctgt gtattcatct ggaccaatga ctctgtgtat ctctaaatga tcatcgactt
    36481 ttacagctct gctcagccaa aaagctgctg tttcatgaat gagcgttttt ccctttgttt
    36541 ctaaaaacga ttgatcctct gttgcttgga catactcttg aacggcataa gcaaggtctg
    36601 cactgatatg atgttctgcg gcagctgaag caacttcttg tcttgttccg gttttgatat
    36661 tcaatgcagc aaatttgggt gtctcttcct gaccagtgtg tgcgctttcc catgggaaaa
    36721 gtgcaccttg atagtgataa tctcttgctt tttgccgtgc gccttctaat cgatcataac
    36781 ggtacagcag caaattcttt gctacttcag gaaactgata taaaaagaaa ggcaggataa
    36841 aaatttccgt atcccagaaa acatgccctt tataaccttc gccagttagt cccttcgccc
    36901 ctacactaac acttggatca ttgcggggag tcataatctg taaatggtaa gcagcgaagt
    36961 ccatcgctaa ctgatcgaaa gaagaatcgc cttggatcgt tatccgatgc tgatgccaaa
    37021 aatctgtcca ataagacatc gactgctcta aaatttctgt ataactaacg cttttctggg
    37081 cttgggcaat acttttctta aacgggtctg tttgctgaag agaggtagag agcacgatga
    37141 tcttctccag cgtaaaggac gtattttttc tgatcttttc tgaaaaggaa gcggtgatcc
    37201 ggcgattttt tgcttggaat acacctgctt tattgaattt agctgcaatc ccaatatcaa
    37261 ttttactttc tgttgtctgg taggatgcac tgatcaaatc gtcaccaaat actcgcagtt
    37321 ctttttcaac caactgctgt gtaccaaaat tggtttgctg tccatcgata cccgttttca
    37381 catctacttg gcagtcttcg gtcaacggct tgatgaccac tttcagggct gcctgatgga
    37441 gattattttg atcagcaaca cgacaagtaa tgacttccac tttttttcct tgagcagttt
    37501 caatgagcgc atagcgttta agctctccca tttctaaatc tagtgcccgc tcgtatgcaa
    37561 gaactttcat cgtctcaaga gaaaaatgtt caccgtcgat ttttatttcc catctgatga
    37621 catctgggag attcactaac tcggctgatt ctgctcctaa aggtcggtta tatacaccag
    37681 ctgcaaacat tccccttact tgattccgat agtcttcctc ctggcttcct ctgataccaa
    37741 aaaatccatt tccaatggtc atctgactcg catacttatt gatatactct aaatcaaact
    37801 gtttttcttc tactttcata aggtaataac ttctcctgtt tctgccgaac gatataaatt
    37861 ttccaccaga ttctggatga catatccttg ccagccatct gcaatcatct gagtctggtc
    37921 accgagcgcc cgattcacaa aatgagccat acttagttca tgtgggtcat taactggcgt
    37981 ggtcttttgt aaggaaacca gctttccatc tacatcttcg aatagttcgc ctggatacag
    38041 attagcacct gcctgatcgc cacaaaaagc cacattcagt ttgtcttctc tgatattcaa
    38101 agcaaacgaa gtattcagtt ggatgatctt gccgttttct aattccagtg tgccaaaaat
    38161 tgcatcttct actgtgaatt tgcttggatc ccatttcccg aatttccctg aatttttttt
    38221 cgtaccaatt ttttggaagc tgtacgcccg aacgcttttt actttaggaa atccagtaat
    38281 atacatcgct gtatcaagca tgtgaatgcc ataatcgatc aaaggcccgc ctccttgaag
    38341 ttctttgtcg ataaacgttc cccagcctgg aacaccagac cgacgaagcg cattcgcttc
    38401 tacgtaataa atatccccga gaacatcaag atgattcttg atcattgttg cttcgtcaga
    38461 aaaacgatgc tggaatccat aagccaaaat ctgcccgttt ttatcggctg tctctgccat
    38521 ctttcttgct tgatcagctg tcatagctgg tggtttctcg cacatcactg aagcattagc
    38581 ttcgagggca gccaatacat gttcgaagtg aaagcgattg ggtgtacaga cactcacgat
    38641 atcgggagaa atctcattca gcatttgtgt aactgatgta tacgcatgag aaaatcctgt
    38701 acgtcggcaa aagtcttctg ctcgctggct gactggatca accacagcgg ccaattctaa
    38761 gacatctgat cttttttgat aataagcagc atgtactttt tctgctactt gtccagcgcc
    38821 tatgattgct actttcttca tttatttctc cctcctttgc ttatttactt attgttattc
    38881 tttctctatt ctttctctaa atcatttaaa tgacgcgctg tttccagaca gtttttcaag
    38941 tattgcaatg attcctgata aattttcact tcattttctc ctcgcaaacg acattcatat
    39001 accatataac cttcatactc attttgaagt agcgtttgaa aatggcgagt aaaatcaatt
    39061 gcgccagatc ctggttggta gcgatgatta tctgctaaat ggatatgacc cacatactct
    39121 ttttgcgtag tcaatgcttt tgaaatatca tcttcttcaa tattcatatg ataaaaatca
    39181 gcgatgatct gcacatgttt caattgatta gagtcaatgt aatgacgcgc atccgctaac
    39241 aaattgatca tatggtcttg gtatcggttc aaaggctcca aataaataac cgtacctgtt
    39301 tcttcggcta ctttatctaa ataacgaagt gattcgctga ctgctttata gtctccttct
    39361 ttgcttcggg gtgaaaccat cggcggtaat cgataagtga acattcccca agctgctggg
    39421 ataacgattc ctttgcctcc aacctcgctt agtgcacgca aaatctcagc aatttgacgt
    39481 acaccattaa gacgccgttc ttcgataaag tcaccgatcc acccatcgta gccgttacaa
    39541 gctgtcacta ctgggatccc tgtttctttg attgcctgtt tgactgtgtc gatattttct
    39601 attaacaact taccgtcgat ttcgtattct tcaaatccac agtctttgat aaatctaaat
    39661 ttttctaaaa tgtctcttgg gaaaaatgct tgatcttgcg taccgaattt cattgttctc
    39721 ctcctcggac ttgaacgccc attttgatac tttcttctgg gtgctggtct acataacgca
    39781 tgtaagcttc tgcgctttct gcaaatggca caactggatc aatgatttct tcacaatcca
    39841 agtaaccgtt catcaataat tcccaacatg tgtcctcgat tcgtttacgg ttccatcttg
    39901 ggtaatctgg gtttggttca ctagatgctc gagcaaagac gatttttgca ttattgaaat
    39961 gtgcttcgcg accaagatta aacccagctg gaaaaggttt agcaaaggca acataagaaa
    40021 tgatcccgcc atagccgatc cctcttaaag aagcttgtaa tgcggcagca ctgccactcg
    40081 tttcaataac tgaatctact ccacgtttat ctgtcaattc ctttagacga aaaccgacat
    40141 ctgctttcat aggatctagc gtgtaatcgg caccgtattt ttctgctaat tctctacgct
    40201 gcgctatggg atcgatccca acgatggtag tagcacctgc ttttttagct aattgaatcg
    40261 caatttgccc gatagcgcct aaaccgaaga ttgctacagc atcacctgct cgaacatgtg
    40321 cttcccggat accacctaaa gcgaattgag caggatcgta acatactgca tttttccatt
    40381 gatccaaccg atcaagttta cgtagtttat aattatcaac accgtttgta atcagcgttt
    40441 cacgaatggg tccataagtg cagactaact ctcctttttg atactctgtc accaaactgc
    40501 ccgtttcttc gatcgtaccg acaatcatat ttcctaattg aaattcgcca aataccacac
    40561 cgcgttggct tccttcttct cttggcaaga ataggcgcca ttcttctgaa aattcttcat
    40621 cgataaatgg acttaatcct ctaaagtcga caatttctgt tcctggtttt ggagtagcga
    40681 aaattactcg gatacgtact tcgttctttt cgatctcacg gtcttcgtaa tctttgaatc
    40741 cggctattct tggttctaat gcaactaatt ttttcatctt ttctttcctc tttcttttct
    40801 ttttatcctt ttacgccgcc ttcgactaaa tcacctttaa taaatcgttc agataacgca
    40861 tacataatga tgactggtaa agcggtcacc aatgaagcgg ccatcattcg tccccaaatg
    40921 tagtctggtg tactgaataa tgtgttcaaa ccgattggta aagtaaattt ttcggaggtc
    40981 gacaggaaaa tcgaagcaaa taaataatca ttccacgaaa ccataaagca ataaacgaag
    41041 acagaaatga ttccagaaat agccaatggt ataatgatcc gaataatgat ctggatccgg
    41101 ctaagaccat cgatcattgc tgcttcttca atatcagtcg gaatcgtgtc aaaataactt
    41161 tttagcatga atatcgcagt aggaagtgtt tgaacgacca tcgtgataat cagtgctgta
    41221 ttggtatcat acaaacctag accggaaatg attttaaata atggaacaac gatcaaaata
    41281 ccggaaaaca tataaacggt gtaaaagcta gcattgattt tcgagcgagc tttgaagcgt
    41341 agttttgaca aagcgtatgc cccgctgatc ccaatcacta ctgcagttac ggaggctatc
    41401 aaggagatca ctaaactatt tttgaaatac atgatgaatg gaaaaatctc agcattgaaa
    41461 atatcctgat aatgttcaaa agtccattct ttcggaaaaa tagtttgtgt cgttgcaatt
    41521 gcttcttgtg aatccttgaa tgatgtcatc agcatgatca aaaatggaaa caaattgaca
    41581 gctaatagca acaaaatcgt cgcgtaaaat aagattttcc tgctcttttt atttttcatt
    41641 tcaagtccac cttctttcgt gagagcaaga cgaccgctgc aataatgatg aacaatagaa
    41701 tcgatatagc tgctgctttt ccaagatcat tgaacgcaaa ggctgtctga tacaaataaa
    41761 cgcctaagat attgacttta ttcgtcagta gatagacatc tgtaaacata tagaacatcc
    41821 agatagatcg taaaacaatc acagttgcta gtactggttt gattgccggc agtgtcacga
    41881 tcttgaactt ctgccaagca tttgcaccgt ccatttcagc tgcttcataa agcgttttgt
    41941 cgactgtttg caaaatagcc aaaaatgaaa taaacgcata cggatagtat ctccagatag
    42001 caaacaaaac gaccataaag aagctagcaa ctggctggtc gaaccacagc ggtgcttcgt
    42061 tgaaaagctg caacctatct actaacagat cattgacgat tccataacta ttattgaaca
    42121 tatatttcca agcaaaaatc aaagaaatag atggcgtaac ataacttaat aaaatcagtg
    42181 aacgcaccgt tttcctaaat ctgaacttgc ggttaagcaa aatagctacg gctaatccta
    42241 aagctgtact gcctgctaca actaagaatg tgtaagcaac tgttaaacct aaagaacgat
    42301 aaaattcact atcacttaaa atttcaagat aattgcttaa tccgataaaa acggcgtctg
    42361 agttaggatt cagcggcata tcgaaaaaac tgatctgaat attggaaagc attggaatca
    42421 gcactaaaac agctaataaa agcatgcttg gtgctaaaag tcccatggct aattttaagt
    42481 cagaatgttt ccttggacga ttcattgaaa cctccccctg ataatctgcc aatagctatc
    42541 tacttttttc gaacaccatt ggcagaactt cttgttattt cgtatttttt tgtgcgtcag
    42601 ctaaagcttg atcgacatct tcgtttccga cagttacttg gttcactgct tttccgatca
    42661 cacctgagct tgtgatatct cccattttcg tgaaattctt ttccccgact agtccaaaga
    42721 cttgtacttc atcaaaagca ctagcaatct cagatggcag ctcgccaaaa gacttgatga
    42781 cttcattttc tttgtaacca tccagttcga ctacttgttt attcactggt tgagcaccac
    42841 caggagacat caagacccac ttttccatgt ttttcggttg agataagaaa gctgtgaatg
    42901 cttcagcagc ctctttttgt ttctcgtcta gaccagaaga aatcgtcaat ccagatactg
    42961 tgccatagac agcttgatct ttttgtgttg ggatagcaaa accgatattt tctgagttcc
    43021 ccgcttcata aacagaaggc aaaatgtaag ttgaatagat tgccattggt gctgttccat
    43081 tcatgaaagc atctttgatt tctgtcacat cattagagcc tggcatcgtg tattgggata
    43141 agttttgata aaacgacaat gcttctctca tcttttccgt atcgatcgtt acctcgcctt
    43201 catcatccaa aacatttgct ccatttgaca aagcaaattg agagaacgct tgctcactca
    43261 ttgttccttc agcagtaggc atagcaattc catattgttt attctcttta tctgtgaaat
    43321 gttgagcaat ttttaaaata tcatcccaat tttctggttc tgaaaaacca gcttctgata
    43381 atttttcttt gtcataccaa ataccttgaa cccagccgct aattggtgca gcaatatagc
    43441 tttttccatc ttctgaacga acaaggtttt tggctccgtc ataataatta tcttctccag
    43501 cttcctcgat aacgttttgg actgccgtct gatcaatcag ttcatcttta tccatcactt
    43561 tagcaaaatc ctgactgact tccattactt ctggtaattt accggaacga gctaacgtaa
    43621 cgatttttgt attgaacgct gattcttcta ccggcacttg cttgattttg atgtctggat
    43681 tttctttttc aaaatcagca accagttcat tgatcacact taatcgttcc tgttcgacag
    43741 aagagtgcat gaattgaatc gttgtctgtt gtgattcgtt agagctttga ctttcaccac
    43801 aagcagtaag aagaaatcca gtagctagca gacaggtagt aacaataatt tttttcattt
    43861 ttttccacct cttattttat tattttcagc cacatatatt catacggctg gaatatcaac
    43921 tttcgttctt gactgcaatg gataatttgt ccagaaaaca ggtctaggta atctcctttt
    43981 ggtaaaagat aacctgctga ttgattggaa aaattatgga taactaagac tttctcttcc
    44041 ttcgcttcgc gaataaaact gataaaagaa tctgctgagt ccagtaagta aatcaaacta
    44101 ttcggatgga aggctttttc tttttttcga atagatagca tctgggtcaa ttcaccatat
    44161 actgctgttc ttaatggatc tgtctgcaac tctttctcaa tatctgctaa caaaaatttt
    44221 tgccgattga tttttcgatt ctctcctgtt ttttctacgc ctttgtaatc atttcgactt
    44281 cccagcacac tttgaatata aattgccggc acgcctggta acgttaccaa aaatgaatga
    44341 gcagctaaaa agcgtttgct tctccattcc gtcgtatctg ttggagaact taacgcatct
    44401 agatacgtag cgtttatctc atatggactg gtcgttccat ctgggttttt cttgtagttt
    44461 actcgggtac cctctttctt cagctgttcc actaattcaa ggatcgtttt ttccggcaga
    44521 atacctcgta ccggattcaa tccgataccg tcatgggagg ctaaaaaatt aaaaaaagtt
    44581 gtttttttcg atgcgaaatc gatggttttt aaccattctt tcaacttact tgtattaccc
    44641 gaacgaatcg cttgaagggt caaaggtggt agcgaaaact gatagaccat ctgtgcttca
    44701 ttctcgccat tcccaaaata actgatatta tctttgtgtg gcacatttgt ctcagtgatc
    44761 aataccgttc ctggagctgc ttcatcagcg attgcacgga ataattggat gattttatgc
    44821 gtttctggca ggtgtataca agaagttcct agttctttcc ataaaaatcc cacagcgtcc
    44881 aagcgaataa atgaagcgcc tttttctaag taaaacaaaa gaatatcgat cattttcaat
    44941 aaaacttctg gatttttaaa attcaagtct acttgatctt cgctaaaagt cgtccatacg
    45001 tgtttttctt tcccgttttt gtcaatgaac gttgtaagta acgggctggt ccttggtcta
    45061 gtcacgctgc ttgtatctgt atccagtggc aaatcaataa aaaaatcttt gtacgtatca
    45121 ttcaagttta aatattcctg aaaccaagta ctttcggcag acatatgatt gcagacaaag
    45181 tcgaacatta accgtgtttc gcttctcatt tcttctatat cttcccatgt gcccaaacgg
    45241 tcgttgattg cctgataatt tacaacggag aaaccatcat cagatgtata tgggaaaaat
    45301 ggcaagaaat gtacaactct aaatgtatct gccaaatact tttgataaaa tttcttaaat
    45361 gtacgcaaag ttggttgctg ctcttcaaaa aattgatccc cataagtaat caagaaaata
    45421 tcttcttcgt cccaatgcag tttgcctgcc tggcctttct tctgctgata aacactgact
    45481 ttttgttcaa tcatagaggc ggttttctca gacaaatctg agtcgcgata aatcgcttgt
    45541 aaaagttttg tatacatcgg attccccttt cttttttggg agcgttccca acgatttgat
    45601 catattatgg aaaacgctta ctgtcaatag cattttttta aataattttg atatacacaa
    45661 aaatacaaat aagaaagctt atgtatcaat aattaatagc ttcttaaaaa aatatggtct
    45721 ctcatgatca tgcaagctat tcctaaattt tcaaaatcat agacaaaaaa gaatgtctat
    45781 tgtactatgg aattattcta aaacgctccc aaaaaggagg tgcatataaa attgaaacct
    45841 actatttatg acattgcgaa aaaagctaat gtctcaaaat caactgtctc cagagtatta
    45901 aataaccagc caaatatctc tgataaagca aaagcaagtg tacttcaagc aatcaaagaa
    45961 ttgaattatg aaccgagcaa aattgcaaga ggactgtcag gaaatggttt tgacgcgatt
    46021 ctagcattat ctcatcgtac ttctcatacg acctatggca atcctttttt ttcagatgtt
    46081 attcaatcca tttctgccgt ttctgaacaa aataacttcg acttgattct acaaacggca
    46141 aaaacaccag aagaagagct agaaaaatgc gcgaaaaaga tccaagaaaa aatgatccga
    46201 ggaattatta ttttaagctc tacgatggat gaaacacttc tttttgaatt ggatcaatac
    46261 aatattccta ttgtggtaat cggaaaagtc ggaaacgact acacgaacat ctactcagtg
    46321 gacacggata atttccagga tagttacgat ttggtggaaa cactgatcaa gaatggacac
    46381 cgtcatatcg gctgtctcca tgctccgaca gaagtttatg tatccaatga tcgtctcaat
    46441 ggttaccgtc aatgcttatt tgatcaccac ctagatatcc gtaatgaatg gattatagac
    46501 ggcggctttc gcttagaaga tagcatggca gcaatgaatg atttaatgaa gctacgagaa
    46561 agaccaactg cgattttttc taccgatgca ttaaaatcat tgagtattta caaagtggca
    46621 gaacaatctg gaaagcagat acctgatgat ctttcgatcg caggattcag tgatccctta
    46681 ttgtcctccc tattttcgcc acagttgact cggatagaca ttccgacaaa tcagttagga
    46741 atcaaagcca cagaactttt attccaacta atccaccacc aataccctga agaaaaaaga
    46801 atcattcttt caaccgagat tactctcaca gattcgatcc aacaacaaga aatgtagaac
    46861 agaattataa aagaacagaa acactgagga taagtcagtt actctgttct tttttcaatc
    46921 actcatccct cgttaccccc tcccggtatt ttagcgaatt tttcttattt tccatctgtt
    46981 ttagatagct gtggataact ataatgtttg tgttaaacta ttttttccac agggaagaaa
    47041 aaactatttt taaccacgtt gtgtataagt ttttatagtt cgttttttta aacacattta
    47101 cccttatttc aatccatgtt atttggggat acttatctct aaaacataaa tagaataaca
    47161 gacatgtcta ctggttttgt tttattcgag gaattattca ataatagcta gcaaaatatt
    47221 aaaaataacc gctctatctt cccatcaatt tattttgcat tcaaaaaatg atctatcttt
    47281 acttttgttg attgacttct cttggaagat gatcgttccg ataccttaat tggatacgaa
    47341 ccgatgttgt taagatcctt tcccactttt cttctagtta gacaaattat tttagacaaa
    47401 ccaaaaaaga ggttgtgaca ataatttgtc acaacctctt ttttaaaata tctattttgt
    47461 ttgattgttc attgcgtctt ctgcagccat ttgcgcttca atatctgaaa tactgtagac
    47521 attttcgctt gctacaccga cttcttttgg ttccatgtca cgataacgtt gcataccagt
    47581 accagctggg atgatcttac ctataataac attttctttc aaaccaagaa gaggatcttt
    47641 cttaccgcgg atcgcagcat ccgtcaagac acgagttgtt tcttggaatg atgcagcaga
    47701 taagaaacta tttgtttcta atgatgcttt tgtgatacct agtaagacag gtcgagaagt
    47761 tgcaggaacg cctcctgaga ctagcgtatt gtagttttga tctttaaagt ctgcgatgtc
    47821 taataatgta cctggtaaga tatctgtatc acctggatcc attacacgaa ctttacgcag
    47881 catttgacga accattactt cgatatgctt gtcgccgatt tctacccctt gcatacggta
    47941 aacacgttgt acttcacgaa gcaagtagtt ttcaacagat aatacatcgc gaacttgcaa
    48001 caattgtttt ggatcgattg atccttctgt caatggtgca ccacggtgga tcatatcgcc
    48061 ttctgctact ttcatacgtg ctgtataagg aacagtatac gtacgtgtat ctgtttttcc
    48121 tttgattgtt acttctttgg tacgtgttgc aggatcttct gagatatcga tgacatcccc
    48181 tgttacttct gtgatcactg cttgcccttt cggattacgt gcttcaaaga tttcttggac
    48241 acgaggaaga ccttgagtga tatcgtctcc ggcaacccca cctgtatgga atgtacgcat
    48301 cgtcaactga gtaccaggtt caccgattga ttgagcggcg atcgtaccaa ctgcttctcc
    48361 gacttcaacg tcagagccag ttgcaaggtt gcgtccgtaa caatgtttgc agacaccatg
    48421 ttttgtatta catgtgaaga cagaacggat tgtcactgtt tcgacacctg catcaacgat
    48481 ttgttttgcg atgtcttcgg aaatcaattg gtttcctgca atgatcaatt catttgtttc
    48541 aggatgacgt acttctttac gggtgtaacg tcccactaaa cgatcttcta atgattcgat
    48601 gatttcattt ccctcgcgga tggcttggat ttcaagacct cggtcagtac cgcaatcttc
    48661 ttcacggatg attacatctt gggccacgtc aaccagacga cgagtcaagt aacctgaatc
    48721 ggcagtcttc aaggctgtat cggtcatccc tttacgagcc ccgtgagttg agataaacat
    48781 ttccaagacg gatagtcctt cacggaagtt cgaaatgatt ggcaattcca tgattcgacc
    48841 attaggagcg gccatcaaac cacgcattcc ggcaagctgt gtaaagttgg aaatgttacc
    48901 acgagctcca gaatcagaca tcatgaagat tggatttttc gcttccaaac cttccatcag
    48961 tttttgttgg atgctgtctt ttgcagcatt ccaaacagca atgacacgtt cataacgttc
    49021 gtcatctgtg atcagaccac gacggaattg ttttgtaata gtttcaactt gtttatgggc
    49081 attttcaatg atttcttgtt tttcattcag tacactgatg tcagcgatcc ctactgtgat
    49141 accggcatgt gtcgaatgtt tgtaccctaa gtctttcata cggtcaagca tcttagaagt
    49201 ttctgttact ttgaatcgtt tgaagacttc tgcgatgatg tttcctaagt ttttcttctt
    49261 gaacggtaat actaattctt gctctttgat atgtgcagga atatcagtac cagcttcaac
    49321 aaagtatttg tccggtgttt ggacggtcaa gttgaagtct gttggttcat tcagataagg
    49381 gaattcagga ggcatgatct cgttgaagat gatcttacca actgttgtga tcatcgtacg
    49441 ttctttttgc cattcagtaa atggtttttc acctaatgag ttagggttca caccgatacg
    49501 tgaatgtaaa tgaacatatc cgttgcgcca tgcgataacg gcttcgtttt gatctctgaa
    49561 gaccattcct tcgccttcgc gtccttcttc ttccattgtc aagtagtagt tacctagaac
    49621 catatcttgg gaaggagtaa caactggttt accatcttta gggttcaaga tgttttgagc
    49681 agctaacatc aacatacgtg cttctgcttg tgcttcttca ttcaatggaa cgtggacagc
    49741 catttggtct ccatcgaaat cggcattata agcttcacat acaagtggat gcaaacggat
    49801 tgcgcgtcct tgtactaaaa ctggttcgaa tgcttggata ccaagtcggt gaagcgtagg
    49861 tgcccggttc agcaatactg ggtgttcctt gattacgtct tctaaaacat cccatacttc
    49921 gtcttcttga cgttcgattt tacgtttcgc atttttgatg tttgaagcta gttcgcgttg
    49981 aacaagctca cgcatcacga atggtttgaa taattcaatg gccatttctt ttggaagtcc
    50041 acattggtac atcttcaaga aaggtccaac aacgataacg gaacgacctg agtagtcgac
    50101 acgtttacct agcaagtttt gacggaaacg accttgtttc cctttcaaca tatgagaaag
    50161 agatttcaat gggcggttac ctggtccagt aactggacgt ccgcgacgac cgttatcgat
    50221 caatgcatct actgcttctt gcagcatacg tttttcattt tgaacgatga ttccaggtgc
    50281 attcaagtct aacaaacgtt tcaaacggtt attacggttg attacacgac ggtaaagatc
    50341 attcaagtca cttgttgcaa aacgtccacc ttctaattga accattgggc gtaaatctgg
    50401 tggaataaca gggataacgt ccataaccat ccagcttggt tgatttcctg aagcgcggaa
    50461 tgcttctaaa atatccaaac ggcggattgc acgtgtacgt ttttgtcctt gtgcagattt
    50521 caattcttct ttcaattcgg ctgcttctgc atctagatca acattatcaa gtaattgttt
    50581 aatcgcttct gcacccatag cagcttggaa ttcttgtcca tattgttcac gtttttcgcg
    50641 atattcgcgt tctgtcaaca attgtttttt ctctaaattc gtatttcctg gttcgatgac
    50701 tacatatgaa gcaaaataaa tgatttcttc caaagcgcgt gggctcatgt ctaatacaag
    50761 acccatacgt gaaggaatcc ctttaaagta ccagatatgt gaaacaggag ctgctaattc
    50821 aatatgtccc atgcgttcac ggcgaacttt cgaacgagta acttccactc cgcatcgatc
    50881 acaaacgatt cctttataac ggatacgttt gtattttccg caggcacatt cccagtcttt
    50941 tgtaggacca aaaatccgct cacagaacaa cccttcgcgt tcaggtttta aggtacgata
    51001 gttaatcgtc tcaggttttt ttacttcacc gtaagaccag cttctgattt tttcgggaga
    51061 agccaaacct atttgcatac tttcgaattt atttacatcg atcaaaaggg gttccctcca
    51121 tttcatttaa tggcaaaccg caagacggct gatcttcgac cgccttgcag ctcatagacc
    51181 ggattagtct aaattatctt ctgcggtttc aaagttttgg atgacatcgt ctttttcatc
    51241 ttccacttgt tcagcagctt tcttttctaa ttctttggca gtttgctgtt cggcaaattt
    51301 tgtcaaagca tcaacagtga tcaaatcgtc gtcttcatcg tccatgtcgc gaagttcgat
    51361 ctctgtttct tcgatatcca agacacgcat gtccagacct agtgattgca attctttcac
    51421 caatacgcgg aatgattccg gtacacctgg ttttggaatt ggttcaccct tgacgattgc
    51481 ttcgtatgtt ttcacacgtc cgactacgtc atctgatttg tatgtcaaga tttcttgtaa
    51541 agtataagcc gcaccgtaag cttccagtgc ccatacttcc atttcaccaa aacgttgtcc
    51601 accaaattga gctttacctc ccaatggttg ttgtgtaacc aatgagtaag gtccgattga
    51661 acgtgcatgc aatttgtcat cgaccatgtg agcaagtttg atcatgtaca tcacaccgac
    51721 agacacacgt ccatcaaatg gttcacctgt acgtccgtcg tacagaacag ttttcgcatc
    51781 gctggccata ccggcttcac gaactgtttc ccatacgtct tcatcacttg caccatcaaa
    51841 tactggcgtt gctacgtgga tacctaactg acgtgcagcc atacccaagt gtaattccaa
    51901 tacttgcccg atgttcatac gtgaaggcac ccctaaagga ttcagcatga tatcgattgg
    51961 tgttccatca ggtaggaatg gcatatcttc ttccggcata atacgggata caaccccttt
    52021 atttccgtga cgaccggcca ttttatcccc ttcatggatt ttacgttttt gaacgatata
    52081 aacacgaact aacatgttta cgcctggtga caattcatct ccagcttcac gagtaaagat
    52141 tttcacatcg tgaacgatac cgccgccgcc atgtggtaca cgtaatgatg tatcgcggac
    52201 ttcacgagct ttttcgccaa agatcgcatg caatagacgt tcttctgcag ataattctgt
    52261 tacccctttt ggcgtgactt tccctacaag caaatcgcca tcttggactt ctgcaccaat
    52321 acggatgata cccatttcgt ctaaatcttt taatgcgtct tccccgacat tagggatttc
    52381 gcgtgtgatt tcttcaggtc ctaattttgt atcgcgggct tctgattcat attcttcaat
    52441 atggatagaa gtgtatacgt catcttttac caaacgacgg ctcatgatga tcgcatcttc
    52501 atagttgtac ccttcccaag tcatgaaacc aactaaaacg ttttggccta gagccatttc
    52561 gccttgttcc atagatggtc cgtctgccag agtatcccct ttttcgactt tctcacccaa
    52621 atgaacgatt ggacgttggt tgtagcttgt tccggagttt gaacgacgga attttgttac
    52681 atcgtattta tctaatgcac cgttgtcgcg gcgtacgcgg atttcagaag catcaacgta
    52741 ttcaacgaca ccgtcatgtt tacataacaa agcagctcct gagtcatgcg ctgatttgta
    52801 ttccatacca gtaccaaccc atggtgcttg aggattgatc aacggcactg cttgacgctg
    52861 catgttggca cccatcaagg cacggttgga gtcatcgttt tccaagaaag gaatacatgc
    52921 tgtcgcaacg gcaactactt gtttaggtga tacgtccatg tagtctactt tatcaataga
    52981 tacttctaag ttttcacttt gagcacgagc cataacaacg tcttgagcaa atgttccatc
    53041 ttcattcaat gggctgtttg cttgggctac gatgtaatgg tcttcgatat cagctgtcaa
    53101 gtagtcgatt tggtcagtaa cacgacctgt ttcacggtct acacgacgat aaggtgtttc
    53161 gataaatccg aatttattta ctttcgcata actggacaaa ctattgatca acccgatgtt
    53221 tgggccttca ggagtttcga tcggacacat acggccatag tgggagtagt gaacgtcacg
    53281 aacttcataa ccggcgcggt cacgagtcaa tccgccaggt cctaaggctg aaagacgacg
    53341 tttgtgtgtc aattcgccaa gagggtttgt ttgatccatg aactgtgaca actgagaaga
    53401 accaaagaat tctttgatac ttgccacaac tggacggata ttgatcaatt gttgaggtgt
    53461 caatgtttca gtatcttgga tcgacattct ttcacgaacc acacgttcca tacgtgctag
    53521 cccgatacgg aattggtttt gtaaaagttc accgactgaa cggatacgac gatttcctaa
    53581 gtggtcgata tcatcgacat tgccgatacc ttccatcaag ttcaagaaat agctcattga
    53641 agcaatgatg tcggctggac gaaccgtttt tacagctgct tctggatagc cgtttccgat
    53701 cacgtttact tcacgttcag ggtctcttgg tgaaaatact ttgatgactt ggacagtcat
    53761 tggatctgtg accacaccgt cttctgatgg atagtaagtc actgaattta ggccattatc
    53821 aatgaaagga gctagtgttt ccatgacttg gtgagtcaga actgttcctt tttcaacgat
    53881 gatctcgcct gtttctggat caactaatgt ttcagctaat gtcagattca atagacgtgt
    53941 tttcaaatct aattttttgt ttactttgta acgaccaacg tttgctaagt cgtaacgttt
    54001 tggatcgaag aaacgagcat tcagcaagtt acgtgagctg tctgctgttt ttggttcgcc
    54061 tggacgcaaa cgttcgtaaa cgtctttcaa accttcttct gttcttgagt cgcttgcgtt
    54121 tttatgcaag tctttttcga ctgtattgcg taatgtttcg ctgtcgccga agatttcaaa
    54181 gatcgtatca tcggaaccaa atcctaacgc acgaactaat acggttaaag gaatcttacg
    54241 tgtacggtcg atacgtacat aagagatgtc ttttgcatca gtttccattt ctagccaagc
    54301 tccacggtta gggataacag tagaaccgaa tccttctttt ccgtttttgt cgacttttcc
    54361 atggaaatag acacctggtg aacggactaa ctgagaaacg attactcgct ctgccccgtt
    54421 gatgatgaat gttccttgtt ctgtcattaa tgggaaatca ccgaagaata cttcttgtgc
    54481 tttgatttcg ccagtctcgc ggtttgtcaa acgcaatgtt acatgtaatg gtgcagagta
    54541 gtttgcatca tgcgcacgag cttctgctac tgtatacttt ggttctttca attcataatc
    54601 tacaaattcc aaagatagat tgccattgaa gtcatcgatt ggcaaaatgt cttcaaacat
    54661 ttctctaagt ccctcatcta agaaccattg gtaagagtct gtttgaattt cgatcaaatt
    54721 tggtaattcc aacacttcac tgattcttgc gaaacttcta cgttcgcgat gttttccgta
    54781 ttttactacg tgtccagcca agctcttcac ccctctaaaa tgtcattcaa aaagactacc
    54841 cgatcaattg ttacaataat atttcaaata ttatttttag gtaacaacag acattttttg
    54901 cggcttttag gcaaaaaaaa accaaaaata gaacattctc ttgaaacctt acttcgtgag
    54961 cttctacttt tgtttcctta tattaaagcc gaaacggaca atatttcgtt tctgcctttt
    55021 cttcatcaga tagtttttgc aacttcatat actacactta ttttctataa aagtcaacac
    55081 ttcctggcgt ttcttctgct ttttcgcaac attttcttaa tgttttcaat tttatgtttt
    55141 caattatctg acaattctta attgacagac ctttttatct cggttatact ttagaaaatt
    55201 cattttgtgg aggaacttct atgcgaacca acatcaacca aatcgcacca acacaactga
    55261 ataactttta ctttagctac tttagatagg tttgtattca tcatgatgga tacaaacgtt
    55321 aaacgacagt ttgtatctat gatggctaaa gaactttgta ttgcagcaaa aagccacgta
    55381 gacaaaatgc taagtggctg agcagtgaaa atacaaagta aaagtatcaa gcattctact
    55441 ttttcaattt attttccgac ttttcaatcc atttggacat tttgtctgaa tggatttttt
    55501 gtttcccaaa attaaggagg aaaaagaaat gaaaaaaaat ataatcatcg gattaggcgc
    55561 tgccctgcta ctagctttag gaggttgcgc acaaaaagac aattccgcag aggcatcaga
    55621 tggacaaaaa acaattggcg tattgcaact tgtacaacat aactcgttag atgctgccta
    55681 taaaggattc aaagaaggat tagcagaaga agggtataaa gaaggcgata atctaacaat
    55741 cgattatcaa aatgcccaaa acaatcaaga caacctgaaa agtatgagtg agaaactcgt
    55801 caaagaagat cctgatttgc tacttggaat cgccactcca gcagcacaat ctttattaaa
    55861 tgaaacaacc actatcccga tgactgtcac agcggtcaca gacttggaac aagcccattt
    55921 agtcacatcc aatgaaaaac ctggcggcaa tgtgactggc acgacagata tggtgccaat
    55981 cgaaaaacaa atcggcttac tgttaaatat cgtgccagac gccaaaacaa tcggcatcat
    56041 gtataacaac ggtgaagcaa actctaagat ccaagctgat ttggcagaaa aagaattgaa
    56101 aaaagcgggc gtcaaagtca aagtactgac agcaaattcc acaaatgatg tccaacaagt
    56161 gacgaaaagt ctagctaaag atgtagatgg aatctatatt cctactgata acacgttcgc
    56221 aagtgcggca agtgttgtcg gtgaagtcgc aaaagaaaca aaaacacctg tcgttgctgg
    56281 cgcagttgat caagtcaaag acggcggtct agcaacagta ggaatcgact acgaaagctt
    56341 gggtaaacaa acagggatca tggcagcaaa aatcttagac ggcaagaatc caagtgaact
    56401 tcctgtcgaa acagcgaatg atttggaact gtatgtcaat aaagacatgg ccaaagcatt
    56461 aggcatcaat ccagacagca tcaaagaagc tgaataaaac acaggaggat tctcatgaca
    56521 aatttgctct ctcttttctt atcatctgca tcgcaagggg tcttatgggc cctacttgcg
    56581 attggtgtat acgtcacttt ccgtatcttg gacattgccg acttgactgc tgaaggaagc
    56641 tttcctttag gtgcagggat cgcggcagta agtattacaa atggctatca tccattgaca
    56701 gcttgtcttc taggcttttt agggggtgcg gcagcagggc ttgtttccgg actgcttcac
    56761 acaaaattga aaattcccgc ccttcttgca gggatcatca ccatgactgg tttatattct
    56821 gtcacatccc gtgtgatggg tgcgccgaac atctctttat tgggtcagcc aacgatcttt
    56881 cattgggtcg agtcattcgg cataacaaaa gacaacgctg tactgatcgt cggtctgctt
    56941 tttgctttga tcgttgtatg tttacttgtt ttgttcttac gtacagaaac aggacttgct
    57001 attcgtgcaa ctggcgataa tctggcaatg agcgaagcaa acgggatcaa cacagataat
    57061 atgaagatca tcggttatat gatttctaat ggatgcattg ctctttctgg ctccttgctt
    57121 gcgcaaaaca acggctttgc tgatttaaat tctggtattg gaacgatcgt aattggactt
    57181 gcttctatta tcatcgcaga agttttgtta cgtaaccagc cactatttct ccgtctcttg
    57241 actgtcgttt taggtgcggt catttatcgt tttattttag cgttagtctt cgaactgaat
    57301 gttgaaccaa gtgattcgaa acttgcttct gctttcgtgc tagtgatctg tctttctttc
    57361 ccgcagatcc agcaaaaatt ccatttacct caaaaaaaga aaggcggtag tgtcaaatga
    57421 atgacgtctt aacattggaa aatctccatc atacctttga aaaaggaacg atcaacgaaa
    57481 atcatgtcct gaaaggcatt aatctttcct tagatcccgg cgacttcgtg acgatcatcg
    57541 gtggaaatgg ggctggtaaa tctactttgc tgaacagcat cgccggtaca ttttctgtcg
    57601 atcagggtca aattttattg aatggaaaaa atatcacgaa aaaatctgtt gtcgaacgtt
    57661 cgaaatccat cagtcgcgta ttccaagatc ctaaactagg gacagccgtt cgtttaacgg
    57721 tagaagaaaa tcttgctctt gctatgaaac gaggaaaaaa acgcggcttc ttccgtggtg
    57781 tcaaaccaca agaccgaaca tttttcaaag accagctggt tcggctaaat ttggggttgg
    57841 aaaatcgttt atcctcagaa atcggtttgc tgtctggagg acaacgtcaa gctattacgt
    57901 tattgatggc cacattacag cgtccagaac tgatcttatt agatgaacac actgcagcgc
    57961 ttgaccctgc tacttccaaa acggtgatgg aattgacaga aaagctgatc actgagcaga
    58021 atctcactgc ttttatggtc actcatgaca tggaagacgc gatccgctat gggagtcgac
    58081 tgatcatgct gcatcaagga aggatcgtcg tagatatcca gcaagaggaa aaacagcagc
    58141 tcacagtgcc ccaattatta gcgttgttca aacaaaacag cggcacggca ttaaaagacg
    58201 atcaagtact tttatcttca taatcagaaa gaggatggga caaaaaatga acgcccaccc
    58261 tcttcttttt tctctttcct tctttcgttt ttgctttatg tatcaaccgt tgtttcttcg
    58321 aaaactattt tgttctctcc ttcttcctga tagaatggat gggaaaggag cttgatgcca
    58381 tgacaacgaa agacaaggta cttcaattat tgaaaaagac accagatttt ctttccggag
    58441 aaaagttggc acagcaactg gaagtttccc ggacaagtat ttggaaagcc atcaaagaat
    58501 tggaaaaggc aggctatcgt tttgagcacg ggcctacagg gtatcgttac ctgccctctg
    58561 acgtactaga tgcaaaagaa atccagcaag agctacggtc gtttattcct gatttgaaag
    58621 tacaaacgat gttgacttct gaatctacga tgaaagatgc caaactggca gcagctcaag
    58681 gagattctgt ttcgaagtta tttgttgctg atacacaaga acaagctcgt ggacgttttg
    58741 gacggccttt tttcgctgaa cctggacgag gaatctacat gagtctcttg ctccatccag
    58801 acaaacggtt tgatgaattg cctcaatata cgatcatcgc agccagtgcc tgtgtgaaag
    58861 ccattgaatc tgtaaccggt cgttctgctt cgatcaaatg ggtcaatgat atctacatgg
    58921 atggaaaaaa agtctgtggg attttatctg aagcagtcag tgatatggaa agcggcagga
    58981 tcagtcatat catcattggg atcgggctta atttttctgt tcctcaaaat cattttccag
    59041 ctgatctcca agaaaaagcc atctctgtct ttcccgacgg acaagctagt gtcacaagga
    59101 atcagctaat cagcaaaatc tggcaagaat tgttcactta tctttctcac ccagaaaaag
    59161 cgttggactt ctataaagaa aaatcattcg tattgggaaa aaccgtgtcg ttcaatcaaa
    59221 aaggaacgac ttatgtcggt cttgcaaaag aaatcggtga tcatggcgaa ctgatcgtcc
    59281 aaacaaatga acagaagatg agtttgtctt ctggcgaaat cagtctcaca tctatccgta
    59341 cgcaataatt atcagatttc acaaaaggac acctgatctc atacagtgtc cttttttctg
    59401 aattctcttc aaaagactct ccttattcta tcagctgaca gtttctctat cctctagaaa
    59461 aaggtattct aaaaatatct ataaagcatg gtacactttc tttaaatgaa tcaatgcaaa
    59521 aaacaagaga gtcatcagtt tacacgatca aaagagatag caggccgctt cctttttcaa
    59581 tcttatagat agaccatggg taacgataaa aacaaaaaag aacttgcgac ttattttgcc
    59641 gtaagttctt ttttagctag tgccttaatc tcttaatccc aacaaacgtc tcgcatagat
    59701 cacctgacca gagtgcatag cagcatcatc aataatagaa acgagacgca ttcctcgtgt
    59761 gactggtggt gtccagttct cgtcaattac ttcatctagc tcggaaggtt ccagccttct
    59821 taagtaagaa atagcaaaat cagcagctcc tcgcaagtat cctagcagat cttctatttg
    59881 attagcttgg atctgctttg cttgttccag actatgcttc caatcttgtt cgttatctgc
    59941 tatcgtcatc ggaaaccggc tttcccagtc ttgactatgc caaattggag aagttcctgc
    60001 taaatcagca atctggaaat ctaattctcg agcggtatgc caagccagcc aagtcattga
    60061 tttgatctgc ggggctctat cttccactgg aaaacgattg gcctcctcaa tagagacgcc
    60121 ttcaaacaaa cgttcaaacc gatcgtatgc ccgatccaca ctctcgcata gcatatctaa
    60181 ataatccatt tattttcctc ctttttattt atcctactta ctttttccta attctaggat
    60241 acctacttat atgttactac acacccttat tttttacact ttaacagtca ccatcatcaa
    60301 tagtttggag attgaaagaa aaagatagtg tcgaactatc aattgaacaa gaagcaacaa
    60361 tatgaacgtt tcattaatat tctttaaata aacaatgatt tttcaaacga acagtttaag
    60421 actaggagaa aaagagtata ataggaaaat gtttgaaagg aaaggaaggg gaaaaatgaa
    60481 aaaaagggca agtttactag tcctagttgc tttgttgttc aatatctttt cgtatggtgt
    60541 ggccgtttca gcggaaacat acattcctaa agaccctggg aatatttata atgatctaac
    60601 tggtaactat ggcttgttag gaattgctag tcaattccac gtatttgcta aaaataagac
    60661 aactgtgaat gcacatacag acggaaatat agcaacacat gaacttgatg caaaaaataa
    60721 ttttggtaca gagcttcatg aaggtttatt gaaccaagaa ataaattata tacaaaaaac
    60781 tgattctttc gctaattctt caggcatacc tacaggtagc acaagaatga ataaatttgt
    60841 cgttggcagt aacgtggatg tagcacttca agatggtcat cctacagtca atggatccag
    60901 gcttgatcac ttaactccag atgattttta taaagatcgt gctgggcatg aatatattga
    60961 tttcgagcag gaatttgcta aattgaatgt agcagcaaat gatcttgcaa cgatcactcc
    61021 agctaaaaca tatactgctg cagattttcc agacatgaac aatcggacaa tcgatttaac
    61081 tgatttatca gataccgggt ttttattggt aaatatcgat gcagaagtgt taacgatgaa
    61141 tacgccactt caaatcatta atccaaatga tcaagtagtt gtatttaatg tcatcaacag
    61201 ttcttctgca ttgaacgtac agtcaccaat caaatacaat gatcgttcaa atcatgaaac
    61261 agaagatttt tcggatgcga atatctcttg gaattttgga aatgagatga ctgatttgac
    61321 aatcagtgct ccttttcaag gaacaatcct tgcgcctaat gctactatcc gcattactca
    61381 aaaccaagac ggaacgatta taggaacaaa tgtgatttta gatgcagcta ccaatcgttg
    61441 ggatcctaac gaaatattta tcagcgacaa tggtacagat acgacagaca caagcgacag
    61501 tacagataca tctaccacaa gtgatagctc agattcatca acttcaagcg acagtacaga
    61561 tactacctct accacaagtg atagcacgga tacatcagct tcaag
//
LOCUS       DACDYI010000006        59686 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.006, whole genome shotgun sequence.
ACCESSION   DACDYI010000006 DACDYI010000000
VERSION     DACDYI010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.006"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..377
                     /locus_tag="IWM52_000358"
     CDS             <1..377
                     /locus_tag="IWM52_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAP9972643.1"
                     /translation="DFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQEMLQGFAKK
                     IKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEENHITASKY
                     HLDDAFFSKSWQHQIPDKLQLK"
     gene            518..1600
                     /locus_tag="IWM52_000359"
     CDS             518..1600
                     /locus_tag="IWM52_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAP9972644.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            1600..2403
                     /locus_tag="IWM52_000360"
     CDS             1600..2403
                     /locus_tag="IWM52_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP9972645.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            2473..3213
                     /locus_tag="IWM52_000361"
     CDS             2473..3213
                     /locus_tag="IWM52_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9972646.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            3224..4228
                     /locus_tag="IWM52_000362"
     CDS             3224..4228
                     /locus_tag="IWM52_000362"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAP9972647.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(4273..6117)
                     /locus_tag="IWM52_000363"
     CDS             complement(4273..6117)
                     /locus_tag="IWM52_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972648.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            6320..6622
                     /locus_tag="IWM52_000364"
                     /pseudo
     CDS             6320..6622
                     /locus_tag="IWM52_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320930.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase"
     gene            6870..7088
                     /locus_tag="IWM52_000365"
                     /pseudo
     CDS             6870..7088
                     /locus_tag="IWM52_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296656.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(7217..8425)
                     /locus_tag="IWM52_000366"
     CDS             complement(7217..8425)
                     /locus_tag="IWM52_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEnfa110 family transposase"
                     /protein_id="HAP9972649.1"
                     /translation="MNCVGIDVSKGKSMIAVMRPFGEVVVSPFEVRHTANELSELAGL
                     LKSLDGETRVVMESTGNYHAPVAWLLHDAGLYVSVVNAMLVHDYGNNSLRRAKTDKKD
                     AVKLANYGLDHWLTLPRYIPEEDTRLMLKICYRQYQQYSKVQTMLKNNLISLLDTTFP
                     DANRLFTSPPRADGSEKWVDFVATFWHCECVCGRSEKAFTTQYQKWCRKHGYNFSEDK
                     ALDIYASACRHFGVIPKTDTAKLLVEQAISQLQTTSSALAALKQEMQSLAASLPEYPV
                     VMGMFGVGPTLGPQLLAEIGDVRRFHSKKALVAFAGIDAPPYQSGQIDVRSRSISKRG
                     SASLRRTLFLVMSVILQCAPIDEPVYQFMDKKRSEGKPYRVYMMASANKFLRIYYASV
                     KAYLESLEHD"
     gene            8715..9680
                     /locus_tag="IWM52_000367"
     CDS             8715..9680
                     /locus_tag="IWM52_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004605046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9972650.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            9702..9914
                     /locus_tag="IWM52_000368"
     CDS             9702..9914
                     /locus_tag="IWM52_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP9972651.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            9989..10465
                     /locus_tag="IWM52_000369"
     CDS             9989..10465
                     /locus_tag="IWM52_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HAP9972652.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            10462..10962
                     /locus_tag="IWM52_000370"
     CDS             10462..10962
                     /locus_tag="IWM52_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003525523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG"
                     /protein_id="HAP9972653.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPS
                     APVRAV"
     gene            11522..13339
                     /locus_tag="IWM52_000371"
     CDS             11522..13339
                     /locus_tag="IWM52_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP9972654.1"
                     /translation="MRNPIHVLKSLEEKASVSNYKYERLYRNLYNPEFYLLAYANIAK
                     SQGSMTQGVDGQTLDNMSLPRINRIIESIRNRTYQPKPAKRKYIPKKNGKLRPLGITS
                     TDDKLVQEVVRMILEAIYEPTFSNNSHGFRPKRSCHTALTQVKKNFTGVTWIVEGDIK
                     ACFDNFDHHVLVELLRKRISDEAFIGLIWKFLKAGYMEQWQYNCTYSGVPQGSGISPI
                     CANIYLSELDNYMQEYKEKYDCEPERRRTTREYERASRRYRKARKALMGAEKSTPELV
                     KEFKDSRRKKMNQHYYNPFEEGFKKIQYNRYADDFVIGVIGSKKDAEKIKEDVKIFLQ
                     EKLHLEMSEEKTKVTHSSKPVRYLGYDFKVIHSKNMKRCKNGDMKRVWYGKVFLYMPK
                     EKWIKKAMERGAIQVKRNNDTGKEMWRPMPRKDLMNRSDAEIVSTFNSEIRGLYNFYR
                     IAENVGALHKYYYMVRYSMLKTLAGKHRTNVSVIKKRHMVNGVLRIPYDTTKGRKYCE
                     FYHDGFRKHSDGYDNVADVMPSYRKYDSRHTIVNRIKAGVCEICGEHADYLCMHHVRT
                     LKSLKGRDIFEQKMLKMRRKSLALCPDCFELLHETKESR"
     gene            13448..14773
                     /locus_tag="IWM52_000372"
     CDS             13448..14773
                     /locus_tag="IWM52_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006771541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP9972655.1"
                     /translation="MQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTIN
                     FSKSMKYNPFVYIHSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIW
                     YEAPAEEMNFSTLLEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLL
                     SAGKTRSSILISCGARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFN
                     FILAMLQSQLINLLCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREI
                     SASIILQSQSQLKAIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTS
                     ENRGSQTSHGLNYQKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYK
                     YLADADKKNTFDVDRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            14850..15011
                     /locus_tag="IWM52_000373"
     CDS             14850..15011
                     /locus_tag="IWM52_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003509345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972656.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKHLMSSM"
     gene            15052..15432
                     /locus_tag="IWM52_000374"
     CDS             15052..15432
                     /locus_tag="IWM52_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnpV protein"
                     /protein_id="HAP9972657.1"
                     /translation="MTISCKAKGDTMKIEYTKVGDYYLPNLYYPEETRPIGLWGMLRK
                     EYLKEHKSGTYTYLLLTARLDSYLADLNEQAQERFELIETQMRSAEGVSEELKRQNPM
                     EWLCHCNNIRNRAAEIIKQELIYV"
     gene            15447..15728
                     /locus_tag="IWM52_000375"
     CDS             15447..15728
                     /locus_tag="IWM52_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972658.1"
                     /translation="MRRELIESYYEEYQRELERFLESDPEMFTLEAEIHHYIEILEPV
                     LRDGGQEMWQTLDRIISAKNAMEGIVAKEMYIRGFLDYERLVCEKDSKI"
     gene            15987..16769
                     /locus_tag="IWM52_000376"
     CDS             15987..16769
                     /locus_tag="IWM52_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAP9972659.1"
                     /translation="MKVKNADEFYKILERKAIYPVFQPIVNLQTGDVAGYEALSRIDR
                     HDTTLMISDLFVIAEQVGCVWKLEKLCRNKALKAAANKPEHAKLFLNVDGNIIQDKSF
                     IQGFTNRKAAKAGVPSCDIVFEITERSDIENYQILQQIMKHYADQGYEIALDDVGAGY
                     SGLNRVVNTSPNYLKVDIELVRDIQKHKKKEIMMEFLLHYCNETGAILIAEGIETEAE
                     LECLCRLGVHYGQGYFLGRPDRTFKNVRNEAMLVLRRLNNKS"
     gene            16942..18660
                     /locus_tag="IWM52_000377"
     CDS             16942..18660
                     /locus_tag="IWM52_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HAP9972660.1"
                     /translation="MNENVKVVFGLIGGLALFLYGMNSMSDALQKAAGERMKKILGFL
                     TRNPIMGALAGALVTAVLQSSSATTVMVIGFVSAGLMSLPQAISVIFGANIGTTMTAQ
                     LMAFKISNYIYPIIFVGFILNFVSKKEKVKNIGMVIFSFGLLFEGIEIMGEVMKPLAG
                     SPVFVDLMGKVSSIPVLGVVLGAVMTLVVQSSSATIAVLQNFASQAGPDGVSSVIGLT
                     GAIPILLGDNIGTTITALLASIGQSKNAKRTAIAHSIFNISGSCVFIFLVPWFAKFVQ
                     FISPKGNEIDVISRQIANAHTTFNIVCTLVWLPLIPLMVKIVTTIIRGNDKTEKAAFE
                     PKYLDMKVIEQPAAAMVLVSKELNRLGELAESLLSDLKTAIVADGDSKTHGSFIENLE
                     IVHQLQDSVTEYITRLFASGNLTEQQSEQTAGLLYVNNSIQRIADRCEDIDQICEKVN
                     NNGKRLTSEASSEMEDCIDIIQQLLKKAMEAIRKGDAVVAETVFKNKKKMHKAEKKYS
                     KAHLNRVKNEVCDASMTRYFSGIMYNFDRMADNCVSIAEEACDNVAFINLDEETGKSM
                     MERGAV"
     gene            18662..19516
                     /locus_tag="IWM52_000378"
     CDS             18662..19516
                     /locus_tag="IWM52_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972661.1"
                     /translation="MVKKKNIRKSALLLIMIMLATGITACGTEEEPLPDLSAMGAVTA
                     VSREEGSGTRAEFENLLKLPESDTGIVVDSTEKVLKKVEEDKNAVGYVAYSSATDTNG
                     KILQINGVLPSEKTIDNNSYPLCRDYYLAYNGELTDVEQDFLTYVKSKGQDIVKQYCI
                     QADSTTTFLSDKSEGKILIEGSTSMEPMVKALADDYQKQNPNAEIEVKATDSSRGITA
                     VISGECDFAMSSRELKDYEAELLETKVIGKDAIAIVINEENPLENLTIKQLTGLYNGT
                     YKNWDDLS"
     gene            20192..20512
                     /locus_tag="IWM52_000379"
     CDS             20192..20512
                     /locus_tag="IWM52_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972662.1"
                     /translation="MPGKEQFMTELKMKEMQLINWFGCPNLIYTRERIHLVAMLTTDE
                     EMKKNLYRVCRFLAREEIAYRYPIMYEMIRKIFSPDDTNPPEACAMFMPDDETDDEEY
                     AMAA"
     gene            20536..20946
                     /locus_tag="IWM52_000380"
     CDS             20536..20946
                     /locus_tag="IWM52_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9972663.1"
                     /translation="MNKVVLMGRLTKNPEIKYAGKDNDMAVARYTLAVNRRYKRDGEQ
                     EADFISCVTFGKSAEFAQKYLHKGMRIVIGGRISTGNYKDKDGKTIYTTDVIVEEHEF
                     AQNKDNGVGADSSETPKTDKDGFMEAPEGEVPFD"
     gene            21093..21299
                     /locus_tag="IWM52_000381"
     CDS             21093..21299
                     /locus_tag="IWM52_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005341250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9972664.1"
                     /translation="MGVSYNRLWKMLIDKNMKRIEMQYLTGISGNILARMGKNQYVSM
                     ETIEKICKKLDCTVDEMLEFTDDE"
     gene            21646..22167
                     /locus_tag="IWM52_000382"
     CDS             21646..22167
                     /locus_tag="IWM52_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9972665.1"
                     /translation="MIRKAILKDVNSIEDTYNEHFQYELNHTAFTVFKKGVYPTKDDA
                     ERAIYAGALFVYEENGTIVGSIIIDKVQPIEYATIPWKEKLSEDEVMVIHLLMVRPSM
                     SGKGIASSLIKFATELAQKNSCRALRLDTGSQNIPALSLYQKNGFEIIASASKKVGDV
                     IAHKNHLFLEKIL"
     gene            22225..22650
                     /locus_tag="IWM52_000383"
     CDS             22225..22650
                     /locus_tag="IWM52_000383"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005423693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP9972666.1"
                     /translation="MIRELRKVDINKVAEIWLDTNIKTHYFISAQYWKSNFELVKELL
                     LQATVYVYEDKQEIQGFIGLSNEYIEGIFVSAEMQSQGIGKILLNYVKGKRNKLILNV
                     YQKNTRAISFYQREGFEIQYSGLDEATGEKDYVMAWQQK"
     gene            22829..23059
                     /locus_tag="IWM52_000384"
     CDS             22829..23059
                     /locus_tag="IWM52_000384"
                     /inference="COORDINATES: protein motif:HMM:NF025559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HAP9972667.1"
                     /translation="MQDDDSGFTYEISRCLYFDTCKEHGYAEFCKVFCNHDWYAYGVL
                     KHHSRFIRKSTIAEDGTVCHDTIEKVTEKHFL"
     gene            23093..23275
                     /locus_tag="IWM52_000385"
     CDS             23093..23275
                     /locus_tag="IWM52_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylan 1,4-beta-xylosidase"
                     /protein_id="HAP9972668.1"
                     /translation="MAKEERFEKIYSQGALTTTEIWVDKETGVNYLYHACGYSGGLTP
                     LLDRDGKPVISQAINR"
     gene            23388..24899
                     /locus_tag="IWM52_000386"
     CDS             23388..24899
                     /locus_tag="IWM52_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972669.1"
                     /translation="MRRSGWTKWLIVIPAMLGIVLVTYVRYTTDIWGFGAFICQLIAI
                     LELAYGLRIAMLAQNRKKSYRLTPEERHEYARYLYEKQYHRYPAVANQMLLVMARMSV
                     LLNNYERAAQELADIRIDKFNPAQLKLYYYLKVVTAMAAGDATGIQESQMCYAGIPDT
                     KCTYPSKAELAMWIEQRAAAQMAEALKKAVPDKKEHPIRISIITLVLAYSTFFYGLWY
                     GIKRDMGYEVRYRFAEISITVITISLAVLLIWGIVSLYRHNCQALSIQFGKNKVALII
                     VYGAAAIFALLIIGMSGLSVVFGMDGTETVTGCDSKYTYITVQEDYGGERRYRTNNPF
                     VMQSMDGLWWSSDTIDEEDNQEEDTTELPDLDTENSTDSEDIQSGNTIEDYETEGQHD
                     GLTIQNAMLAVYNYLQEQNTLQNMAFSYTANAKGEVYAVVSETQETKNGNTVNVRYCL
                     YDNGSKTDADGNSCEELVLEKVYPDGNYETELVDFYLVNADTMQVTDEQKNTW"
     gene            25222..25446
                     /locus_tag="IWM52_000387"
     CDS             25222..25446
                     /locus_tag="IWM52_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3791 domain-containing protein"
                     /protein_id="HAP9972670.1"
                     /translation="MSEKTEITFMQTRLIRLASEEWHLPVEQIIHLFKEADVLGYIEK
                     CYGIFHCEGDEAVLEDITEFLQRKGIEISA"
     gene            25439..26005
                     /locus_tag="IWM52_000388"
     CDS             25439..26005
                     /locus_tag="IWM52_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
                     /protein_id="HAP9972671.1"
                     /translation="MLELKDGFVLYHGSYCEVKEPDLAKCAKRKDFGQGFYLTTSKEQ
                     AESFLRTSIAKAIATGTIEEGQKFGYISTFEFKLSENLETHIFENADVDWLHCIAAHR
                     KKKMFIEVEREMAKYDIIAGKIADDATNATLTAYLAGAFGTAGDKVADDFCIRQLLPN
                     KLKDQYCFKTEAAIECLKFVKGEKIWLK"
     gene            25993..26223
                     /locus_tag="IWM52_000389"
     CDS             25993..26223
                     /locus_tag="IWM52_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972672.1"
                     /translation="MAEVTIPKEKMNYTIDLLITMVTDEIAEETGKDRNEVLTDFLCS
                     KTGKALYDEKTKLWCTGPAYIAELYMEELKKS"
     gene            26424..26789
                     /locus_tag="IWM52_000390"
     CDS             26424..26789
                     /locus_tag="IWM52_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="HAP9972673.1"
                     /translation="MSRAKNPKYESMSLEELKANMEKSRQELEAAIHHKNILEQRKKL
                     VERRERSHRLIVKGAEFEKVFPLSRDLEQEDVQDMMNQLQNSSYNKSIVRQAHIAALH
                     KDQQQIAEAVKKTEKGDDS"
     gene            26789..28516
                     /locus_tag="IWM52_000391"
     CDS             26789..28516
                     /locus_tag="IWM52_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HAP9972674.1"
                     /translation="MALYHFSVRNVSRGKGQMVVASAAYISGQRIFDSYYNKIHDYTS
                     KSGVIFTEILTPEYVPERLTDRETLWNEVEHVERNNKAQLAYSFDIALQNELTLEENI
                     RLAKEFCQEQFVARGMIVDLAVHEGKAENEEEPDNPHFHVLVPIRPFTEEGIWGNKQR
                     REYILDEDGNRVKDAKGKDMFNAVSTTGWNDPELLKEWRRAWTEKVNGKFRECHMASR
                     IDHRSYKEQGIDLIPTIHEGYEVRAMEKKGIKTVIGELNRAIRQFNQMFISLKESIQW
                     MKTAYEEMKAELDRRQNPTLLESLQDYYDKKTQGRPPLPNFYAEMKQRGKNLSNLQEF
                     AKSISYLQTHQIETMDDLQERIEELNGVVSVSKKEISEKRKQLKELENLEKMAEVIKT
                     NQPLIDEYNHFYFQKRRERYYQQHKKEINYYRKCERELKQHLDKNGKVPTARWKREKE
                     ELQAGIEELKADNQPYQEELTFVKKVQSCADIARRDREMAEADTSGRSEEKREKQVKF
                     PTFYTVQAKEILEENGKAEQQPLNQSEQNPEKKTSLLRKLDEKKKECAERDAKQQAVK
                     KKRNYDMSL"
     gene            28546..30462
                     /locus_tag="IWM52_000392"
     CDS             28546..30462
                     /locus_tag="IWM52_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9972675.1"
                     /translation="MSKMQYTEEQIIRANQTDLVSFLSAQGEQLGKSGKEYRWKKHDS
                     VTVSGNRWYRHSQGRGGYPVDFVMEFYNATFPEAVKMLTGEEGEGRNSTCPAPSPDFR
                     LPEKEENNDRIIRYLTENRGIEKNMVEEWIGSGDIYEEKKHHNVVFVGRDADGIPRYA
                     HCRGTGETKYRGDVAESDKSYGFCHRGTDNQLFVFEAAIDLLSFIQLFPKDWKKRSYL
                     SLGGISSAALMAFLSERPQITSVFLCLDNDHAGNEASEKLAIEIPDGYSVIRLKPSRK
                     DWNEILCDKNADRKKSIIETVTMKVPEKEELVPMLCYEDIEQTSVEWLWFPYLPFGKL
                     TIIQGNPGEGKTYFAMMLTAACTNRKTFPNMEEIEPFNVIYQTAEDGMGDTIKPRLVE
                     AGADLSRVMVIDDTEEALTLSDDRIEKAIRQNQVRLLIIDPVQAFIGADVDMNRANEV
                     RPVFRKLGMIAEKTGCAIVLIGHLNKSSGTQSTYRGLGSIDIMAAVRSLLFIGKVKKD
                     PTTRVLIHEKSSLAPPGETMAFKLGDEEGFRWVGAYEISADDLLDGKEGKPTETKLQR
                     GTKLIYELLADGNAVTIRELDEKAKAQGISQRTMREARSRMKEELDYRMNEKQENTIR
                     LKKQGRMGDGRILE"
     gene            31147..31329
                     /locus_tag="IWM52_000393"
     CDS             31147..31329
                     /locus_tag="IWM52_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972676.1"
                     /translation="MAVKKKIINIRVYFDGDQDAKEVFAEVIAQKIRDTKTKDKLAIM
                     KDKQYNDYEFSNLKSA"
     gene            31356..33053
                     /locus_tag="IWM52_000394"
     CDS             31356..33053
                     /locus_tag="IWM52_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9972677.1"
                     /translation="MNNRYMSNNIERNATWRVGVYCRLSKDDELTGESASISNQRDIL
                     VNYCQSQGWQVVDVFQDDGYTGLNTDRPDLQRLLKACEKGLVNLVITKDQSRLGRNHV
                     QTGFLMEEFFPKHGVRYIALYDNVDTFAGDNEIAPFKNVLNEMYSRDISKKVHASYHL
                     QATKGKFTGVVPPLGYQKDPEEKGHLLIDDETAPIVRKIFQWAVDGHGINFISRELER
                     QQIPCPSWWHRKRGLRTYYTKWEKKDPENGKYVWDESYLKSLLMNPVYCGDMASQKRY
                     YRFKIGNQGDKAPEDWITVRDTHEAIIPQDVFDLVQAKMKGRKRQTEQGEYSMFAGLL
                     RCAECGGALTLKKTHTKDQHEVYTCSTYIHKGKAHCTQHRVDADDLYDAVLTRIQECA
                     KAVTGDGTELENRVKELCEEDTQGHRESLEKLVSKQKDRLETLDRLIAKLYDDLINDK
                     ITESVFDKMLEKTQKEQTDIKKELFQNESVLNTEEKLDAQSQQWIDDISEYADIKELD
                     ANLLNRLISKIVISEPQEKDGTENYCRTPETVTMEIHFNLKPIPELGTIERGSGSHK"
     gene            33078..33209
                     /locus_tag="IWM52_000395"
     CDS             33078..33209
                     /locus_tag="IWM52_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005927774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972678.1"
                     /translation="MLIFSRLISSKYFLIKDIKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            33301..34164
                     /locus_tag="IWM52_000396"
     CDS             33301..34164
                     /locus_tag="IWM52_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972679.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            34195..34737
                     /locus_tag="IWM52_000397"
     CDS             34195..34737
                     /locus_tag="IWM52_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HAP9972680.1"
                     /translation="MFISTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVE
                     RLASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTR
                     GNAEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFAR
                     QKTPGWDVWGNEVECDLILT"
     gene            34751..35173
                     /locus_tag="IWM52_000398"
     CDS             34751..35173
                     /locus_tag="IWM52_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAP9972681.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            35103..37502
                     /locus_tag="IWM52_000399"
     CDS             35103..37502
                     /locus_tag="IWM52_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAP9972682.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            37534..39525
                     /locus_tag="IWM52_000400"
     CDS             37534..39525
                     /locus_tag="IWM52_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAP9972683.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            39548..39799
                     /locus_tag="IWM52_000401"
     CDS             39548..39799
                     /locus_tag="IWM52_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HAP9972684.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            39789..41018
                     /locus_tag="IWM52_000402"
     CDS             39789..41018
                     /locus_tag="IWM52_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HAP9972685.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            41015..43096
                     /gene="topB"
                     /locus_tag="IWM52_000403"
     CDS             41015..43096
                     /gene="topB"
                     /locus_tag="IWM52_000403"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9972686.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            43245..47165
                     /locus_tag="IWM52_000404"
     CDS             43245..47165
                     /locus_tag="IWM52_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HAP9972687.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            47166..48110
                     /locus_tag="IWM52_000405"
     CDS             47166..48110
                     /locus_tag="IWM52_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972688.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            48114..48317
                     /locus_tag="IWM52_000406"
     CDS             48114..48317
                     /locus_tag="IWM52_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972689.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(48617..48853)
                     /locus_tag="IWM52_000407"
     CDS             complement(48617..48853)
                     /locus_tag="IWM52_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9972690.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(49036..49407)
                     /locus_tag="IWM52_000408"
     CDS             complement(49036..49407)
                     /locus_tag="IWM52_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972691.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(49404..50732)
                     /locus_tag="IWM52_000409"
     CDS             complement(49404..50732)
                     /locus_tag="IWM52_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP9972692.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(50693..51022)
                     /locus_tag="IWM52_000410"
     CDS             complement(50693..51022)
                     /locus_tag="IWM52_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP9972693.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(51280..51651)
                     /locus_tag="IWM52_000411"
     CDS             complement(51280..51651)
                     /locus_tag="IWM52_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9972694.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            51796..51981
                     /locus_tag="IWM52_000412"
     CDS             51796..51981
                     /locus_tag="IWM52_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9972695.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            52924..53583
                     /locus_tag="IWM52_000413"
     CDS             52924..53583
                     /locus_tag="IWM52_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HAP9972696.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            53586..54929
                     /gene="vanS"
                     /locus_tag="IWM52_000414"
     CDS             53586..54929
                     /gene="vanS"
                     /locus_tag="IWM52_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP9972697.1"
                     /translation="MERKGIFIKVFSYTIIVLLLVVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQNKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLGNIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            55100..55906
                     /locus_tag="IWM52_000415"
     CDS             55100..55906
                     /locus_tag="IWM52_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HAP9972698.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            55924..56751
                     /gene="vanW"
                     /locus_tag="IWM52_000416"
     CDS             55924..56751
                     /gene="vanW"
                     /locus_tag="IWM52_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HAP9972699.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATIPEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            56748..57719
                     /locus_tag="IWM52_000417"
     CDS             56748..57719
                     /locus_tag="IWM52_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAP9972700.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTARGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            57712..58740
                     /gene="vanB"
                     /locus_tag="IWM52_000418"
     CDS             57712..58740
                     /gene="vanB"
                     /locus_tag="IWM52_000418"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HAP9972701.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKFG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQIIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            58746..59354
                     /locus_tag="IWM52_000419"
     CDS             58746..59354
                     /locus_tag="IWM52_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HAP9972702.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
BASE COUNT        17376 a        12893 c        15222 g        14195 t
ORIGIN      
        1 ctgattttca gctggtcttg gatcaattag caaaaaacca taacgaagta ttgttcatca
       61 tcccaccggt taacgagaaa tggagcgatt ataccggact atctcaagaa atgttacaag
      121 gatttgccaa aaagatcaaa tttcaattaa atagccaagg attcaatcga atagctgatt
      181 ttgtcaatca agcgggtacc aactatttta tggaagacac gatccatcta ggctggaaag
      241 gctggttggc tgcagatcaa cagatcagac cgtttttaga agaaaatcat attactgcta
      301 gtaagtatca tcttgacgat gcttttttta gcaaatcttg gcaacatcaa ataccagata
      361 aactacaatt aaaataaaga aaacaggcga gtgttgctcg cttgttttct ttatttttga
      421 atattctgaa taaaagataa aaataccaag tttgcctgaa caaaaatcat aagcaatggt
      481 ataatcacaa tatgaatata gaaatggggg aaaacatttg gatacaaaag aagaacagct
      541 acttattgat ttaacttctg caaaaggagt acctggaaat gaagaagagg taagagaagt
      601 attcagagaa tatgccaaac cattcgctga tgatattttt tatgacggtt taggcagtgt
      661 gattgccaaa catggtgctg gcggaggccc caaggtcttt atttctggac atatggacga
      721 agttggtttt atggtaacaa aaataaccga aaaaggattc ttggaattcc aaactcttgg
      781 aggttggtgg gggcaagtca tgcttgctca gcaagttgaa atcaaaacac gtgaagggaa
      841 aatcgtccac ggtgtgattg gttccaaacc accacatgtt ttaacacctc aagtaagaaa
      901 taaaccttat gaaataaagg atatgttcat tgatattggc gcatcaagcc aagaagaagc
      961 caaagagtgg ggcatacgtc ctggagatat ggtcactcca tatattgaat acaaacgaat
     1021 gaatggcagt aaatatttac tggcaaaagc atgggataat cgtatcggga cagctgtttc
     1081 tctgcgagta ttagagaatc tatctaaaga agcacaccca aatgttttat tcgcaggaag
     1141 cgatgtgcaa gaagaagtag gcttgcgagg tgctcgaaca agtacacacc ttgtaaatcc
     1201 cgatattgct tttgctttag atactggtac agcaggagac actccaggaa tgacaccaaa
     1261 agaagcagat tcgattttag gaaaaggccc acagattttg atttttgatg catctatgat
     1321 cccgcataaa aaattattga attttgttat tgatgtagca gaggaaatgg atattccatt
     1381 ccaatataca gtgatttctg gcggaggaac tgatgcagga caaatgcatt tgacaagaga
     1441 tggtgttccg tctgtagcca ttaccgttcc tgttcgttat ctgcattccc atacatcagt
     1501 gattcatgaa gatgattatt tgaatacagt gaaactagtc acagaattag tgaaacggct
     1561 tgatagtgaa acagttgaaa aaattcggag ttatgaataa tgaataatag aatacctatt
     1621 tctgaatatc ggaaaaaagc acgagtttct ttggaaaatc aatggggaat caatacttgg
     1681 cttgttttct tatctataat atttacgagc gtcatccaga acttttttga cggtttcagt
     1741 ccttttccgg aagaaagcat gcagcagcac ttattgagtt ttctagtcga taatttactt
     1801 ttattcgcat tcaactatgc attgttttac attgcgctag tagttgtacg gggtggcaga
     1861 ccaaaaagta accttttatt ttcagttttc caaagaaagt attttggttc gatacttggc
     1921 atcaatttga tcaattcagt catcaactgg ctactcaaca gtttaatctt tctgcccatg
     1981 tttttaatca gcggagtgag tacttatatg cagctgcttt tcagttcaaa tgcggtacct
     2041 acgggtgtca tgccagaaga ttttcttgat atcagctttg ttttatcgat gatagggatg
     2101 atttttgttt cagcactcat catgagtatt gttggcggtt tattccaatt tgctgcatgg
     2161 acaaagttcg actatcctga tttgtcggtt attcaatgtt tgaaatatgg ctggttttta
     2221 ttgaaagata gaattggtac gtatattttg ttgcagctgt cttttatcgg ctggtatttg
     2281 ctaggagcaa tcgctttgtt catcggtctt ttgtgggtca ttgtttatgt gaatgtgtca
     2341 gttgcagaat tttatgaaca agcacggatt gaaaaaggag gtccagacgc ctattttact
     2401 tagcatgtaa gcagtttatt catcaaaaaa ataacttttg tggtaggata aatctttgag
     2461 ggggaatgta cgatgaaatt aactgtttta ggatgcttag gagcgtatcc ttataaaaat
     2521 caagggacaa caggttattt gcttcaatct gacgggttca atttgttgat cgatgcaggt
     2581 agtgctacgc tgatcaaatt acaagaacac cttgatccac ttgatctaga tgctgtgatc
     2641 cttagccatt atcatcatga tcatattgct gaccttggtg tattacagta ttactggcaa
     2701 cttcatccta agagaaatcc ggagacattg ctttcaattt atggacacac aaaagattcc
     2761 attcactttg aagaattgac gttggaaaat gtctcaaaag gacatcctta ttttgaagct
     2821 gaagaattaa aaattggtcc ttttctgatt acttttatgg aaacaatcca tccagtagta
     2881 tgttacgcca tgcgtatcgt agaagaaaca acggggaaag tattcgtatt tacaggtgat
     2941 tcaggttatt tggaaagttt cgctgatttt gcgaaagatg ctgatttgtt tctagcagat
     3001 acctatcttt ttgaaggaaa tgaacatcat catgcccatt tcacttcaaa agaagctgga
     3061 gaacttgcga aatcagctaa cgtaaagaaa cttgtactta cacacctacc tcagtttggt
     3121 gatttagaac aattgaaaaa tgaagcgcaa aaggcagcag gcaaagggat tgatatagaa
     3181 cttgctgcag ttgacaaagt gtttaacatt taggaggaag aacatgattt ttgtaccaaa
     3241 tgaaaaccac gacccaagaa tcaatttagc aattgaacaa tatttgctgc aagaaatgcc
     3301 tttagatgaa cctattttgc tcttttatat caatgaacct tcaatcatca tcggcagaaa
     3361 ccaaaatacg cttgaagaaa tcaatcgaga ttatgtggaa gaaaatggca ttcatgttgt
     3421 ccgtcgctta agtggaggcg gagctgtcta ccatgatttt ggaaacctga actttagttt
     3481 tatcatgcct gatgatgggg attcattccg tgactttgcg aaagtaacac agccaatcat
     3541 ccaagcactt catgaattgg gtgttgctgg ggcggaattg aaaggccgaa atgatctagt
     3601 cattgatggc atgaaatttt ctggcaacgc gatgtatgct accaacggtc ggatgtttgc
     3661 tcatggaaca attatgttcg actctgatat caatgaagta gtcaatgcat taaaagtaaa
     3721 aaaagacaaa atcgaatcaa aaggaatcaa atcgatccgt tctcgtgtaa cgaatatcaa
     3781 accatttctt ccagaagaaa agcaaaaaat gacaacagaa gaatttcgtc aagaaatctt
     3841 gttgaaaatt ttcggcgtag agtcagtaga agaagtaaaa acgtacgaat tgactgatga
     3901 agattggaaa aaaatcaatc gaatctctga ggaatattat cgaaattggg attggaatta
     3961 cgggaagtct ccagatttca atttctctcg ccaaaaacgt ttttctatcg gttcgattga
     4021 agtccatttg aatgtctcag aaggaatgat caaagaagca aaaatctttg gcgatttctt
     4081 cggactagga gatatctctg acgtggaaaa aaaattggtt ggacagaaat atgataaagc
     4141 atctctatca aaaatagtca gccaaattga tgtgaagaaa tattttggtg cgatagaacc
     4201 agaagaattg cttaccttac tttattgaaa ataaaaaata gcggaatcca catatatgga
     4261 ttccgctatt ttttatacag gattcgggta atttcgagag agttgaacag tgatccgatc
     4321 aatgaaagta tctttttcct gcgttagtgt tcctgtgtaa cgatccgcta cgtctttcat
     4381 catcattaag tcaaaacgtt cctgatcatc taattcttct gtgatgcctc gactaaatag
     4441 gtttgtatcg taatgtgcac tatgttcgca acaaatgacg aaatgttcct cttgttcgtg
     4501 tatcgacaaa taaatttttc gatacagtgg attttcgatg gcatgagttt ctctaaagga
     4561 atgctctaaa gcgtggatca aaagttgagc cagatcgtca tcgttgatag gaagttcttc
     4621 gggaagatct gcttggaagg ttatctgaat attccgtttg gcagctaact tctggtaacg
     4681 cgctaagata agatttgtca cctggtggtt gcaaaaagaa cacagcattt cgaaattttt
     4741 tgcttcttct aatatctttt ccacatagtt tttagctttt gtttggtccg tatgcattaa
     4801 atcttgcaga ttctccatct cttggacaaa ttccaggcga agtgtattta cttcttggat
     4861 atgctgtcga agctgctcat aatgttgctc caacaaatca tttttgattt ttaagaaatc
     4921 tacttgtctc cgatctttgt cggtatttaa gatgtgatcg attagattgt agctgataat
     4981 aacgattaga atgataatat ataaaatcgt agaccaattg atcgttgttt gatagatacc
     5041 taatgccgtt tggatgtaaa tcaagcaatg gataatggct accaagacga tccaaggtgt
     5101 acaagctaca taaaaacggt tattcttata tgcttcgcca atgcatgcaa gtgaagtgac
     5161 taaagtgctt ataacactag caatattagc aatcggcatt aattcaggaa gttctgcagg
     5221 agtaaaagtt gcataaagca gaacgccgat aaaaatcaaa agttggaacg tacaaaaaat
     5281 tccgtaccat tttttatagt aaaacattcg gctccaatag tagcagatga tgaaagctga
     5341 cgttaagata ataaataaat gagataaaaa agaatgaacg aatggttcaa aaagaatacc
     5401 acttaaaata tcttctgata cagcttcaaa tcctaatgcc aaagcaaagc aacttaagag
     5461 aataagtgag tattcaagac gtttttgttt atacatcaag tagatcgtat agaaaaaggt
     5521 ccctatagcg ataaaaacag cgattatcat cgtcaatact tgcggcactg attgtgaata
     5581 aataaaacta atcaatggtc cagttgttcc tagataaact ttcggtggca gtccagcata
     5641 gttctgataa ggagatgaga ctgtaagggt caattttttt ccgatataat ttgctggtaa
     5701 atctatcaca gccaatgaga gtccgggatt gctttcttcc tgataatctt tccgttggta
     5761 taaaatcttt tcgcctagat atacagtaat ccattgatgg tttcctttga ttagtaaatt
     5821 tgcttcgtcc aacggttttt caagtgtcgt ttccataatg aatgtttcgt ctttatccac
     5881 ggtaggaatc gtacgagtgt acttgctgtc aaacttaaaa tcaggatcat cttttgtata
     5941 gaaaacccac gaatctttta tttctttcat ttgattgctg tccgcaggag aaaaaataaa
     6001 ataaaagaaa taagcaattc ctgttaaaaa taacaatgat aatataatga ttatgctcca
     6061 ctttatacgt aaataaaaag attctttttt attatatatg ttattaaaaa aagacataaa
     6121 ggtattcctt tctaaaactt gtaatgttag ggtaaacgtt ttttaaacta acgtcaatta
     6181 tttataataa aaaatgagat aaagatgagg aaatagaatt agaaacatat aattattaaa
     6241 gggaaacatt tttacagcta taatttgaaa aaaatttatc gttattatat gattaagaag
     6301 gattgaataa agaaagaagt tgaaaacgat acctgattac aaaaaaataa cttttataag
     6361 ttctataacg ttgattatat ttttaattct tattcttctc gtcgtcttgg ttccccgaat
     6421 ttttggtttg actccttata ttattgagga caaagaaatg agtccatatt atgcacaagg
     6481 aaatctgata tatgtacgac cgaaaaatac aaatgagatt ttagtcggag atattattac
     6541 atattatgaa aattcaggaa aaaaaatctc gacgcgtcgc gtaatagccg ttgaaaacaa
     6601 ccacgaagat ttttcctcaa aattttaggt tatcgctgaa agccccggaa atacgggcat
     6661 ttcgcaggat atgagaactc aatttactac caatttacta ctttcggatt gagccgcata
     6721 tatgctgaac catactgcta atacccgcca ccccaaatac ggcacatcat aaattgaata
     6781 cgacaccgta aacgcggcgt tcctctttcc cgtcatggga gaacaggacg ccgctttttt
     6841 atgcccaaat agagaaagga ggcgacgcca tgctatttac ttccggccct gaccgggcgt
     6901 ttgaacggct gatgcaggaa aagcccggtt tcgatcacta cgacggcggc ggggcggacg
     6961 cgccggagga ttgcggcacc tgccgatttt accgcccgga ttggaaatat caattctgcc
     7021 agtttgcaga gtgtccctat cagccgggca agctgaccga ctatgcagcg gtcaaattca
     7081 cagtgaaaga cctgctaata aaagtcaagt agaaatttca gatttttagg gaggcgaaaa
     7141 aatgcaatac aaaatcaagc cgctgagcat ctcaaaatta aaacggcggc cagccagatg
     7201 gtatagagtg taaaaatcag tcgtgctcca gggattccaa gtaggctttc acagaagcgt
     7261 agtagatgcg caggaacttg ttggcagagg ccatcatgta gacacgatat ggcttgcctt
     7321 cagagcgttt cttgtccatg aactggtaca ccggctcgtc tattggagca cattgcagga
     7381 tgacgctcat caccagaaag agcgtcctgc gcagtgaggc agatcctcgc ttggaaatgc
     7441 tacggctacg gacatctatt tgaccggatt ggtagggcgg ggcgtcaata cccgcaaagg
     7501 caaccaacgc ttttttggaa tgaaaacggc gtacatcgcc aatttcagcc aggagttgag
     7561 ggccgagggt tgggccaaca ccaaacatcc ccatcactac aggatactcc ggcagagaag
     7621 ctgccaggga ttgcatctcc tgtttgagag cagctaacgc ggaagaagtt gtctggagtt
     7681 gagaaatggc ctgttctacc aaaagttttg ccgtatccgt tttcggtatg acaccgaagt
     7741 gtctgcaagc agaggcataa atatccagcg ccttatcctc gctgaaattg tagccgtgct
     7801 ttctgcacca cttctggtat tgggtagtaa aggccttctc agaccggcca caaacgcact
     7861 cgcaatgcca aaaagtagcg acaaagtcca cccacttctc gctgccatcg gcgcggggcg
     7921 gactggtaaa caagcgattt gcgtctggga aggtggtgtc cagcagggag atcaggttat
     7981 tcttcagcat ggtctgaact ttggaatact gttggtactg ccggtagcag attttcagca
     8041 tgagtcgggt atcctcctcc gggatatatc tcggcaatgt gagccagtgg tcaagaccgt
     8101 agttggccag ctttacggca tcctttttgt cggttttggc ccgtcttaaa ctgttgtttc
     8161 cgtagtcatg caccagcatt gcattgacta ccgagacata aaggcccgcg tcgtggagca
     8221 gccaggccac cggagcatgg taattacccg tggattccat caccacacgg gtctcgccgt
     8281 ccaggctttt gagcagtcct gccagctcgc tcagttcatt ggcggtgtga cgtacttcaa
     8341 agggtgaaac cactacctct ccgaagggcc gcatgactgc aatcatgctc ttacccttgg
     8401 aaacatcgat gccaacgcag ttcattcaca ttcctccaat acaaagaatc tgcaaccgga
     8461 atccatcttt tctcgctgcc gattcaatct attgtgtgac acgaactctc cgacatccgg
     8521 tggttcaacc tgcttaaatc gaacgctgca acgagaggat ggctaacagt ctttcacacg
     8581 gacatacaag cccaaggagt gattggtcag ccagttgctc ctctcattgt agcttaggca
     8641 cgagatggag ggaaagccgg actggctgtc cggctttcct gtccaaattt attgtaatag
     8701 gagcgaacaa cgctatggca gttttccgtg tggaaaagaa caaaggctat acggtgatgt
     8761 caaatcacca cctgcggaat aagaacctga ccttaaaggc caagggcctg ctgtcgcaga
     8821 tgttgtcctt gccggaggat tgggactata ccctaaaagg actgtcctat atcaaccggg
     8881 aaagcatcga cgcgatcagg acagcggtat gggagcttga aaaagctgga tatatccggc
     8941 gtgagcaggg ccgtgacgca aaaggcaaaa tggccgatat ggtctatacc atctacgaac
     9001 agccggtatt ggaaaacccg gtattggaaa atccaacatc ggataacccg gtgttggaaa
     9061 atccgacatc ggataatccg gcgtcggaaa atccaacgca attaaataaa gaagtacaaa
     9121 gaactgactt atcaaaaaaa gaaaaaataa ttacagatgg acaaagtacc cattccattc
     9181 ctatcccatc cccgacccct gcccctatgg agggcgaggc agcggctacg ccgccggaac
     9241 ggaaaaggac aggaaaggat gaggctgtac ggatttatcg ggaaatcatt ttggagaaca
     9301 tcgagtatgc ctatctcatt caggacagga gcattgaccg tgaacagctt gacgaaatcg
     9361 tagacctgat gctggaaacc gtctgcaccg cccgaaagac aatccgtgtc gccggggacg
     9421 actaccctgc cgagctggtg aaatccaagt acatgaagct gaccggcgaa catatccgct
     9481 ttgtccttga ctgtctgcgg gagaacacaa cgaaagtccg caatatcaaa caatacctgc
     9541 gggctatgct gttcaacgcc cccagcacca tttccaacta ctacacggcc cttgtcgcgc
     9601 acgacatggc acagcccgat tggggaaagc ctaaatccgg cctgccggac tattcctgcg
     9661 gcgagggcga aagcctgtaa cgagaaaagg aggacgaccc tatgaagcag ggcgcattga
     9721 tttttgacga gcgaacagac cgttacgaca tccgctttga tctggccgac tattacggcg
     9781 gcctgcattg cggggagtgt ttcgacgtga tgatcggcgg caaatggagg cccacccgca
     9841 ttgaaatggc cgaaaactgg tatcttgtcg gcatccgggc cgacgacctt tccggcctgc
     9901 gggtgcggat ttgacctata tctacctgcg gcgtctggtg gttccgcctt agacgccgct
     9961 tttgactttc tgacaaggag ggatgcgatt gcaggaagaa gttactcaaa agaccattgc
    10021 cctgtccatg aaaacgggca agctcacggc tcaagtgctg caagcggcgc tgaaaaagta
    10081 cctgcaacac cgggccaagg gcaaaaacac gctgcaccac ggccagcaga gcctaaagca
    10141 gcttaaaaag catggggctg atctctccaa cattgagatc accgaggcca acattggagc
    10201 gttcaagcca tgcgccaaga aatacggcgt ggattttacc ttacgaaaag ataagaccac
    10261 ccagccgccc cactacgttg tgattttcaa ggccaaggat gcagataacc ttgatctggc
    10321 gtttcgggag tttaccgcca agacgctttc caaggagcag cggccctcta tccgcaaggt
    10381 tttgtccgct gtgaagcaaa aggcggcggc ccagaccaag cagcgggcca aggagaaaat
    10441 caaggaaagg gggctggaac tgtgaagcct gacgtaaaga aattgctgat cctgaacctc
    10501 ccgtatctga tttttgtgta cctgtttgcg aaatgcgggg aggcgtaccg gctggccgct
    10561 ggtgctgacc tgtcggggaa gctcctgcat tttgcggatg gttttgcggc ggcttttgcc
    10621 aacccgctgc caagcctgca cctgtttgac ctgtgtatcg gtatcgccgg tgccgtgatc
    10681 gtgcggctgg tggtgtactg caagagcaag aacgtgaaga aataccgcaa aggcatggag
    10741 tatggcagcg cccgctgggg gacggccaaa gacatagcgc cttacatcga ccccaagttt
    10801 gaaaataata tcctgctcac ccaaacggaa cggcttacca tgaccggcag gcccaaagac
    10861 cccaagacag cgcggaataa aaacgtgctg gtgatcggtg gcagcggttc agggaaaacg
    10921 agatttttcg tgaaacccag tgcgcccgta agggcggtat aaatcctacc ttgagcaagt
    10981 agtaggtaaa agtatcaagc ggcattgtgc cgcaacctat cattccccgt cttaccccaa
    11041 aagggaaacc ggagggcgac catagcatga cggaggacac ggggcgctga agcctcagaa
    11101 gcgccggcgt gacggaatga aaatccacgt atgaggatgg aagctagact gcttgaatga
    11161 cagcctgaaa tcgggaccaa gagcgtaccc ctgacgtaga gaggttgtac tcgtcagtgt
    11221 tcctgacagt cgcattgttt ggctatgcgg ctgctgatac tcggagcagg ttcagccacg
    11281 ggaaagtgga aaaaggcgaa cgtcacatac cgacaacgtg gaacgcttgt ttataccgta
    11341 ctaaacgggg attgcctaaa gtgaaatgcc gaaaggctat gaaaacgctg aaatgcgggg
    11401 ttctgaatat tcaccatggc aacagagttc ccatagtagt ctgcggacgg gaaagccgtc
    11461 tacatggcaa aggggaacag tgaatcattt tcaaaacaga aaggatggtg tgtgagacac
    11521 tatgagaaat ccgattcatg tcttgaaaag ccttgaagaa aaggcaagtg taagtaacta
    11581 caaatatgaa agattatatc gcaacctata caatccagag ttctatctcc ttgcatatgc
    11641 gaacattgcg aaatcgcagg ggagcatgac gcagggggta gacgggcaga cgctggacaa
    11701 catgagcctg ccgagaatta accggattat tgaatccata cgcaacagga catatcagcc
    11761 aaagcctgca aaaagaaagt acattcctaa aaagaacggg aaacttcgcc cgttgggaat
    11821 cacttctact gatgataagt tagtgcagga agtggtcaga atgattcttg aagcaatcta
    11881 tgagccgaca ttcagcaaca attcacacgg tttcagacca aaaagaagct gtcacacggc
    11941 acttacgcaa gtgaagaaaa acttcacagg tgttacatgg attgtcgagg gagacattaa
    12001 ggcgtgcttt gataacttcg accatcatgt gttggttgaa ttactgcgga aaaggatttc
    12061 agacgaggct tttatcggtc taatctggaa gttcctgaaa gccggatata tggaacaatg
    12121 gcagtacaac tgcacctact ccggtgttcc gcagggcagc ggtatcagtc cgatatgtgc
    12181 aaacatctac ctcagcgaac tggacaacta tatgcaggaa tacaaagaaa agtatgattg
    12241 cgagccagaa cgcagaagga cgaccagaga atacgagcgg gcgtcccgga gatacagaaa
    12301 ggcacgtaaa gcgttaatgg gcgcagaaaa atcaactcct gaactggtaa aagaatttaa
    12361 ggattccaga aggaagaaga tgaatcagca ctattacaat ccgtttgagg aaggcttcaa
    12421 gaaaatccag tacaaccgtt acgccgatga ttttgtaatc ggcgttatcg gctccaaaaa
    12481 ggacgcagaa aaaatcaagg aagatgtaaa aatctttctc caagaaaaac tgcatttgga
    12541 aatgtccgag gaaaagacga aagtcaccca ttccagtaag ccggtacgct atctggggta
    12601 cgatttcaaa gtaatccatt ccaagaacat gaagcgttgt aaaaacggcg acatgaaacg
    12661 ggtgtggtat ggaaaagtat tcctgtatat gccgaaagaa aaatggatta aaaaagcgat
    12721 ggaacgggga gcgatacagg tgaaacgcaa caatgacaca ggcaaagaaa tgtggcggcc
    12781 tatgccaagg aaagacctga tgaaccgcag cgacgcagag attgtgtcta ctttcaattc
    12841 cgaaattcga gggttatata acttctatcg gatagcagaa aacgtaggcg cattgcacaa
    12901 gtattactac atggtgcggt acagcatgtt aaaaactctg gcaggaaagc acagaacgaa
    12961 tgtcagtgtt attaagaagc ggcacatggt aaacggagta ctgagaatcc catacgatac
    13021 aactaaggga cgtaaatact gcgaatttta ccatgatgga ttcagaaagc acagcgacgg
    13081 ctatgacaat gtggcagacg ttatgccgag ttataggaaa tatgacagca ggcacacgat
    13141 agtaaaccgc ataaaagccg gagtatgcga gatttgcggg gaacatgcag actatttatg
    13201 tatgcaccac gtaagaacgc tgaaatcgct gaaaggcagg gatatatttg agcagaaaat
    13261 gctgaaaatg agaagaaaat ctctggctct ctgccctgac tgctttgaac tcttacacga
    13321 aaccaaagaa tcaaggtgat tcatggaaag ccggatacgc tgagaggtgt acgtccggtt
    13381 tgggaggcgg ctccctgaaa cctactgatg aaagtcagca aggcgcaggg tgcctacctc
    13441 atacctgatg caatgctttc cgaccacgga ctatccgact tcgtttgtgg tgacagatcc
    13501 gaaaggcact ttggttctgg aaaccggcag gatgttccag caggcgggct accgcattaa
    13561 aatcctgaat accattaact tttccaagtc catgaagtac aacccctttg tctacatcca
    13621 ttcggaaaag gacgtgctaa agctggtaaa taccctgatt tccaacacca agggcgaggg
    13681 ggaaaagtca gcggaggatt tttgggtgaa gtcggaacgg ttgttttatt cggccctgat
    13741 cggctatatc tggtacgagg ccccagcgga ggaaatgaac ttctcaacgc tgctggaaat
    13801 gataaacgcc agtgaagccc gcgaggacga cccggagttt caaagccccg tcgatctcat
    13861 gtttgagcgt ttggagcaga aagaccccga ccattttgcg gtgcgccagt acaagaaatt
    13921 cctgctgtcc gcaggcaaga cccgttcttc catcctgatt tcatgcggtg ccagacttgc
    13981 gccctttgac atccgggagg tacgcgagct gatggaggac gacgaaatgg agctggacac
    14041 catcggggac gaaaagacgg tgctgtatct cattatgagc gatacggaca cgacctttaa
    14101 tttcattctg gctatgctcc aaagccagct aatcaacctg ctttgtgacc gggctgatga
    14161 tgtgtacggc gggcggctgc ctgtccatgt acgcctgatt ttggatgaat ttgccaacat
    14221 tggccaaata cccaatttcg ataaactcat tgccaccatt cgaagccgtg aaatatcggc
    14281 ctctattatt ttgcagagcc agtcgcagct aaaagcgatt tacaaggacg ccgccgagat
    14341 catttcggat aactgcgata gtgtgctttt ccttagtggg cgcggcaaaa atgccaagga
    14401 gatttccgat gcgctgggga aagaaacgat tgacagtttc aacaccagcg agaaccgggg
    14461 aagccaaacg tcccacggat tgaactatca aaaattagga aaggagttga tgtcccaaga
    14521 cgaaattgcc gtgatggacg gcggcaagtg tatcttgcag ctacggggtg tgcgcccgtt
    14581 tttatcggag aaattcgata ttaccaagca tccccggtac aaataccttg ccgacgccga
    14641 caagaagaat acctttgacg tggacagatt tttgactatc acgcgccgga aacggcagca
    14701 agtggtaacg caggatgaag tattcgacct gtatgaaatc gacctgtcgg acgaggacgc
    14761 cgccgctgaa taaacagcgg cttttttcat tccaacaaca aaatcaaaaa atcattatga
    14821 gccggaaacc ggcagaaaga ggtaaattta tggcattttt taattccgca gttactactt
    14881 tgcagactat cgtgatcgga ctgggcggcg ctctgtgcgt atggggcggc atcaatctgt
    14941 tggagggata cgggcaggac aatccggggg caaaatcgca gggtgtcaag cacttaatgt
    15001 cttctatgtg aaaagatttt aaagagaaaa tgcacatcct acgcagataa catgacaatt
    15061 tcatgcaaag cgaaaggaga caccatgaaa atcgaataca caaaagtggg agactattat
    15121 ttaccgaatc tttactaccc ggaggaaacc agaccgattg gcttatgggg aatgcttcgg
    15181 aaggaatatc tgaaagaaca taaatctgga acttatacat atcttcttct gaccgccagg
    15241 ctggacagtt atttggcaga tctgaatgaa caggcacagg aacgctttga attgatcgaa
    15301 actcagatgc gaagtgcgga gggagtttcc gaggaactga agagacagaa tcctatggaa
    15361 tggctatgcc actgtaacaa tatccgcaac cgggctgctg agatcatcaa gcaggaactg
    15421 atttatgtat aagggggatt ctagatatga gaagagaact gattgaaagt tattatgaag
    15481 aataccagcg ggagctggaa agatttcttg aatctgaccc ggagatgttc actctggaag
    15541 cagaaattca tcattacata gaaatactgg aaccggtcct cagagatggc ggacaggaga
    15601 tgtggcagac actggacagg attatttcag cgaaaaatgc aatggaggga attgtggcaa
    15661 aagaaatgta tatccgggga tttctggatt atgaaagact ggtgtgtgaa aaagacagta
    15721 agatctgaat aatcagaaca tagactatat ataacgaggc ttttattgta tagacagtaa
    15781 gagcctcatt taatatagtt ttcataataa aaaacaggtg gtagtacgta tttgctttgt
    15841 gttatataag aattggtctg gctgaacaat ggtgaaaatt tactatgatt ttacttaaat
    15901 ctgattgaag gatgaaaaag tgatgatatc atatagtggg ttgatagggt tacagaaaat
    15961 gtattcacag aggcagagaa gaaataatga aagtaaaaaa tgcagatgaa ttttataaaa
    16021 tattagaaag aaaggctata tatccggtat ttcagccgat tgtaaattta cagactggag
    16081 atgtggcagg atatgaagct cttagtcgta tcgacaggca tgatacgact cttatgattt
    16141 cagatttgtt tgtgattgct gaacaggtag gctgtgtttg gaaattggaa aaattgtgca
    16201 gaaataaagc attgaaggca gcggcgaata aaccagaaca tgcaaaatta tttcttaatg
    16261 tagatggtaa tatcattcag gataaatcgt tcatacaggg atttacaaac cgtaaggctg
    16321 cgaaagccgg agtaccgtcc tgtgacattg tatttgaaat aacggaacgg tcggatatag
    16381 aaaactatca gattctgcaa cagattatga agcattatgc agatcaggga tatgaaatag
    16441 ctttggatga tgttggagct ggatattctg gtctgaaccg tgtagtaaat acatcgccaa
    16501 actacttaaa agttgatata gaactggtca gagatattca aaaacataag aaaaaggaaa
    16561 ttatgatgga atttctgttg cattactgta atgagacagg agcaattctg attgcagaag
    16621 ggatagaaac agaagcagag ttggagtgtc tgtgtagatt aggtgttcat tacggacaag
    16681 gatattttct tggaagaccg gacagaacgt tcaaaaatgt aaggaatgag gcgatgttgg
    16741 tattacgtag actgaataat aaatcgtaat gaaaactata tgtcagcagg tgaaaaatga
    16801 ataagtcaat tataacatta taaagatttc cattgatttt tacacctatt ttacacaagc
    16861 actcacatag atttgatttc tgtcttctat aattgtatag gaaaatgtag atcttatata
    16921 ataacaggag aggaaagaga aatgaacgag aatgtgaaag tggtttttgg tctgatcggt
    16981 ggccttgcct tgttccttta tggaatgaac agcatgagtg atgcacttca gaaagccgca
    17041 ggagaacgaa tgaaaaaaat ccttggattt cttacaagaa atccaattat gggagcactt
    17101 gccggagcac ttgtaacagc tgtattgcag agcagttcag cgacgacagt tatggttatc
    17161 ggttttgtca gtgcgggact tatgagtctt ccgcaggcaa tttctgttat ctttggtgcc
    17221 aacataggaa ccactatgac ggcacagctg atggcattta aaatcagcaa ttatatttat
    17281 ccaattatct ttgttggatt tattctgaac tttgttagca aaaaagaaaa ggttaaaaat
    17341 atcggtatgg taattttttc attcggactt ttatttgaag gaattgaaat catgggtgaa
    17401 gtcatgaaac ctttggcggg aagtccggtc tttgttgatc tgatgggaaa agtttcatcc
    17461 attccggttc ttggtgttgt actgggagcg gtcatgacat tggttgtgca gagtagttca
    17521 gctacgattg cagttctgca gaattttgca tcacaggcag gaccggacgg agtaagcagt
    17581 gtaatagggc ttaccggtgc gattccgatt cttcttggag ataatatagg tacaacgatc
    17641 acagcacttc ttgcatcaat tggacaatct aaaaatgcaa aacgtacagc aattgcgcat
    17701 agtattttta atatcagtgg aagctgcgtt tttattttcc tggttccgtg gtttgcaaaa
    17761 tttgtccagt ttatttcacc aaagggaaat gagattgatg tgatttcaag acagattgca
    17821 aatgcacata caacgtttaa tatcgtctgt acactggtct ggctgccact gattccgctg
    17881 atggtaaaaa ttgttacaac aatcatccgt ggaaatgaca aaacagagaa agcagctttt
    17941 gaacccaaat atctggatat gaaagtgatt gaacagccgg cggctgctat ggttctggta
    18001 tctaaggaac tgaaccgact tggagaactg gcagaatctc tgttaagtga tttaaaaacg
    18061 gcgattgtag cagatggaga ctcaaaaact catggttcgt ttatagaaaa tctggagatt
    18121 gtacatcagt tacaggacag tgtgacagaa tatattacca gattattcgc aagcggaaac
    18181 cttacagaac aacagtctga gcagacggca gggttactgt atgtaaacaa tagtattcag
    18241 aggattgcag acagatgtga agatattgac cagatatgtg aaaaagtaaa taacaatgga
    18301 aaaagactga ccagtgaagc ctcctcagaa atggaagact gcattgatat tatccagcag
    18361 cttttaaaga aagcaatgga ggcaatccgt aagggtgatg ctgtagtggc tgaaacagtt
    18421 tttaaaaata agaaaaaaat gcataaagca gagaaaaaat acagtaaggc acatttaaac
    18481 cgtgtaaaaa atgaggtttg tgatgcgtct atgacaagat atttttctgg gatcatgtat
    18541 aattttgacc gtatggcaga taattgtgtc agcattgcag aagaagcctg tgataatgtg
    18601 gcttttataa accttgatga agagacggga aaatcaatga tggaaagagg tgcagtataa
    18661 aatggtaaag aaaaaaaaca tccgaaaatc tgcacttctg ctgataatga taatgctggc
    18721 aacgggaata acagcatgtg gaaccgagga agagccattg ccggatctgt ctgctatggg
    18781 agctgtcaca gctgtatcac gagaagaagg gtcaggaacc agagcagaat ttgagaatct
    18841 tttgaaactg cctgagagtg atacaggaat agttgttgat tctacagaga aagttctgaa
    18901 aaaggtggaa gaagataaaa acgcagttgg ttatgtcgca tatagttccg caacagatac
    18961 gaatggaaaa atacttcaaa tcaacggagt tcttccttct gagaaaacaa tagacaataa
    19021 cagttatcct ctttgcaggg attattatct ggcttataat ggagaactga cagatgtgga
    19081 acaggatttc ctgacttatg taaagagtaa aggacaggat attgtgaaac aatattgtat
    19141 tcaggctgac agtacaacaa cattcctttc tgacaagtca gaaggaaaga ttctgattga
    19201 aggatccact tcaatggaac caatggtaaa agcactggca gatgactatc agaaacaaaa
    19261 tccaaatgct gaaatagaag taaaagcaac agattcttca agagggatta cggcggtgat
    19321 aagtggagag tgtgatttcg caatgtcatc cagagaatta aaggattatg aagcagagct
    19381 tctggaaaca aaagtcattg gaaaagatgc tatagctatt gtgataaatg aagaaaatcc
    19441 actggaaaat cttacaataa aacaacttac aggtctgtac aatggaacat ataaaaactg
    19501 ggatgatctt tcctgagaaa aacgaatata atatcttaca agcactgact ataacggtcg
    19561 gtgcttcttt tttggatttt ttcaaaatta tctgacattc ttcaaattcc cacgtgtgat
    19621 tagtggaaaa ataatttttt ctgcaaaagt accgtccaaa tgccctctga aacttgctat
    19681 tagtgaggga atatttcatt tatgcgaaaa aaacttgcaa aagggggttg gtaaataccg
    19741 cttcccaggc tttataggta gaggaacaat tattttaaaa attttacccc aaagatgtcc
    19801 gcttttgcct gtctcagtac agagtatgtg aagggactaa caaaagtaaa agaaaaaatg
    19861 ataaaaatgc aaaaaaatat cacgaatctt ttgcatcttg cgaagtcatt tgactctagt
    19921 aatcagagga cgttttggaa tgttccttcg atgaaccttg acaaccgata agtgcaccct
    19981 agaagcctaa caggagttat gccatgacct ttctttttct gagagcgagc gaaattataa
    20041 tgacactccc gatgatgggc ggctgaccga agccttatgg tcagtgacct gctgatgcag
    20101 gcagaggcag tctgagacgg ctgcaggtga cagggaaacg cacgccagac cgccggacga
    20161 atgaaactga tgacaatgtt tacagggcaa aatgcccgga aaggagcagt ttatgacaga
    20221 actgaaaatg aaagaaatgc agttgattaa ctggtttgga tgcccgaatc tgatttatac
    20281 aagggagaga atccatctgg ttgccatgct tacaacagat gaagaaatga aaaagaatct
    20341 ttacagggta tgcagattcc ttgcaagaga agaaatcgca tatcggtatc cgatcatgta
    20401 tgaaatgatc cggaaaatat tttcaccgga tgataccaat ccaccggaag catgtgcaat
    20461 gtttatgccg gacgatgaaa cagatgatga agaatatgca atggcagcgt agctgcggaa
    20521 aggacagtaa cgattatgaa taaagtggtt ttaatgggaa gactgacaaa gaatccggag
    20581 atcaaatatg ccggaaaaga taatgatatg gcagtagcaa gatatacact ggcggtaaac
    20641 agaagatata aacgtgacgg tgaacaggaa gcggatttta tttcctgtgt cacattcgga
    20701 aagagtgcag agtttgcaca gaaatatctt cacaagggaa tgaggattgt gattggcggt
    20761 cgtatcagca ctggcaatta taaggacaag gacgggaaaa caatttatac tacagatgtg
    20821 atcgtggaag aacatgaatt tgcccagaac aaggataatg gtgttggagc tgattcatcg
    20881 gaaacaccga aaacagataa ggacggattt atggaagcac cggaaggtga ggttccattc
    20941 gactaaaccg aagtaaatgg ttggagaggt tgccctttgt cggggcatcc tcttttccat
    21001 tttacagaag acatcaaaaa ttacaggtat tcaacctaac gaaatcgtga tataatcaaa
    21061 atatgttcag atgaaaaata tggaggttgc ttatgggcgt aagttataac agattatgga
    21121 aaatgctcat agataaaaat atgaagcgga tagagatgca gtacctcacc gggatcagtg
    21181 gaaatattct tgccaggatg ggaaaaaacc agtatgtatc aatggagacg attgagaaga
    21241 tctgcaagaa gctggactgc accgttgatg agatgctgga atttacagat gatgagtaac
    21301 aagagcagac atgtcaaatt attaaaactg gaagaatgct tcagaagtcg aaaaaagaaa
    21361 ttgaaaaaag ataacacgcc ggatacggca taaatctctt agcaacgcta agagcgcact
    21421 tctatacagg caattccatc tgcctgtatc gtgcgacctg cggtgctgtt cccgatttca
    21481 agaaatctca aaccgggaaa tacagcgaaa tcagagattt cgtacaaggg gaagatcccc
    21541 ttgcggcaca gatgcctatc cctgataaag tttgtgactg aatatttcag atatagacat
    21601 ataaggggaa agaaagtgtg aaatcaaaat ttgtggagag attttatgat tagaaaagca
    21661 atactaaaag atgttaattc gattgaagat acttacaatg agcatttcca gtatgaactg
    21721 aatcatacag cctttactgt atttaagaaa ggggtttatc caacgaaaga tgatgcagaa
    21781 cgggctattt atgctggtgc tttgtttgta tatgaagaaa acggaacaat tgttggaagt
    21841 atcattattg ataaagttca accaatagaa tatgcaacta ttccttggaa agaaaaatta
    21901 tctgaagatg aggtaatggt aattcacctt ttaatggtac gtccgagtat gtctggtaaa
    21961 ggaattgcat catcactaat aaaattcgct acagaattag cacaaaaaaa tagttgcagg
    22021 gcactacgtt tggatacagg aagtcaaaat attcctgcgc tatctttgta tcaaaaaaat
    22081 ggatttgaaa taattgctag tgcttctaaa aaagttggag atgtaattgc acacaaaaac
    22141 catctttttc tcgaaaagat attgtagttc gtaaattcca gtttgaaaaa ttgagaaaat
    22201 cggagtttga ggagcatcac cgttatgatt agagaattac ggaaagtgga tataaataaa
    22261 gttgcagaaa tatggttaga tacaaatata aagacacatt attttatctc tgctcaatat
    22321 tggaaaagta attttgagtt agtaaaagaa cttctattac aggcaactgt ctatgtttac
    22381 gaggataaac aggaaataca gggttttata gggttaagta acgaatacat tgaaggtatt
    22441 tttgtttctg ccgaaatgca atctcaaggt ataggtaaaa ttttgctaaa ctatgtaaag
    22501 ggaaaaagaa acaaattaat cttaaatgta tatcaaaaaa atacacgggc aatatccttt
    22561 tatcaaagag aaggatttga gattcagtat agcggtttgg atgaagctac tggagaaaaa
    22621 gactatgtaa tggcatggca gcagaaatag aaattcaagt tttagtgttt ggaaaattat
    22681 gacttagtgt gcaagttggg agaaaatatg gtaacggaaa aagagaaaaa agcatgtcga
    22741 tttattgccg aatggctata agattgtgag acagtctctg gtagatgatc tggagggcga
    22801 aaaaaaggta tgttgggaga cagaaatttt gcaggatgat gatagtggat ttacttatga
    22861 aatcagcagg tgtctgtatt tcgatacctg taaggaacat ggctatgcag agttctgcaa
    22921 agtattttgc aaccatgact ggtatgctta tggagtatta aagcatcatt cgagatttat
    22981 cagaaaatcg acgattgccg aagatggaac tgtatgtcat gatacaatcg agaaagtaac
    23041 agagaaacat tttttataaa accgaaaaat tgattatggg aggaaattga aaatggcgaa
    23101 agaagagaga tttgaaaaaa tatattctca gggagcatta actactactg agatatgggt
    23161 ggataaggaa actggtgtga attatttata tcatgcatgt gggtattcag gtggactgac
    23221 tccattgttg gacagagatg ggaaaccagt tatttctcag gctataaata gatgacctta
    23281 gaaacagcaa ctgttatggt aagtaacagc atcgtcacaa tatgtatatg gtcgattggt
    23341 gtgtgcggta atatactata ttcagaaaaa caattcagga gatttttatg agaagatcag
    23401 gttggacaaa atggttgatc gtgattccgg caatgctagg cattgtgttg gtgacctatg
    23461 tgcggtatac aacggatatc tggggatttg gtgcatttat ctgccagctg attgcaattt
    23521 tggaattggc ttatggtctg cgaattgcga tgctggcaca gaatagaaag aagtcctatc
    23581 ggttaacacc ggaagaacga catgagtatg cacggtattt atatgagaag caatatcacc
    23641 gttaccctgc tgtagcgaat cagatgcttt tggttatggc acggatgagt gtactcctta
    23701 acaattatga acgggctgca caggagctgg cagatattcg tatagataaa ttcaatccag
    23761 cacagttgaa gttgtattat tacctgaaag tggttacagc aatggcagca ggagatgcga
    23821 cagggattca agagagccag atgtgttacg cagggattcc ggatactaaa tgcacatatc
    23881 cgtcaaaagc agagcttgct atgtggatag agcagagagc ggcagcgcag atggcagagg
    23941 cactgaagaa ggcagtgccg gataaaaaag agcatccgat acggatcagt atcattactt
    24001 tggttcttgc gtatagtacc ttcttctatg gattgtggta tggtataaaa cgtgatatgg
    24061 gatatgaagt gagatatcgt ttcgcagaaa tatcgattac tgttattaca atcagtctgg
    24121 cggttctttt gatctggggg atcgtgagtt tatacagaca caattgccag gcattatcca
    24181 tacaattcgg taaaaacaaa gtggcattga taattgtcta tggagctgct gcgatatttg
    24241 cacttttgat tattggaatg agtggtctga gtgtagtgtt cggaatggat ggaacagaaa
    24301 cggtgactgg ttgtgacagc aaatatacgt atataactgt gcaggaagat tatggtggtg
    24361 aacggagata cagaaccaat aatccatttg ttatgcagag tatggatggt ctgtggtggt
    24421 cttcggatac aatagatgaa gaagataatc aagaagagga tactacagaa ttaccggatt
    24481 tggatacaga gaattcaaca gattcagagg atattcagag tggaaatacc atagaagatt
    24541 acgagacaga agggcagcat gatggtctga caattcaaaa tgcaatgctg gcagtttata
    24601 attaccttca ggagcagaat acacttcaaa atatggcgtt cagttacacg gcgaatgcaa
    24661 agggagaagt ctatgctgtc gtcagcgaaa cgcaggaaac aaagaacggt aatacggtaa
    24721 atgtgagata ctgtctgtac gataatggtt ccaagacaga tgcagatggt aattcctgcg
    24781 aagaactggt gttagaaaaa gtatatccgg atggcaatta cgagaccgaa ttggtagatt
    24841 tttatctggt aaatgcagat acgatgcagg tgacagacga acagaagaat acgtggtaaa
    24901 ataaattctg gggaagtagg tgcattgtat gacaatgaga gaaaaaataa aactattatc
    24961 gtaaatgttt caagcggact gggttctttt ggaagtatgc aaaaggactc caagacataa
    25021 gaggtgaact gcgctgatcc aggtgccacg gcaacagatt tgaatgtcga cggaggaaag
    25081 caaacagtac aggcgcaatc gtaaaacttg caatgattga taaaaccgga ctaacagtaa
    25141 cttttattag tcgatttgga aatatgcctt ggtaatcaac aattccggtt ttactgacta
    25201 agaaaatgag gaggattcat aatgagtgag aaaacagaga ttacttttat gcaaacaaga
    25261 ctaatccggc ttgcttcaga agagtggcat ttacctgttg aacagattat ccacttgttt
    25321 aaagaagccg atgttcttgg atatatagaa aaatgctatg ggatttttca ttgtgaaggc
    25381 gatgaagctg tgcttgaaga tattactgaa tttttgcaaa gaaaggggat agagattagt
    25441 gcttgagtta aaagatggat ttgttctcta tcatggaagt tattgtgaag taaaagagcc
    25501 agatcttgcc aaatgtgcaa agagaaaaga ttttggacag ggattttatt taaccacatc
    25561 aaaggaacag gcagaaagtt ttctgagaac ctcaatagcc aaagccatcg caaccggaac
    25621 aattgaagag gggcagaagt ttggatatat ttcaactttt gaattcaaac tttcagaaaa
    25681 tcttgaaacg catatttttg aaaatgcaga tgtggactgg ttacattgta tcgccgctca
    25741 ccgaaagaaa aaaatgttta ttgaggttga acgtgaaatg gcaaaatatg atatcattgc
    25801 aggaaagatt gcggatgatg caacaaacgc tactcttacc gcttatcttg cgggtgcttt
    25861 tgggactgca ggtgataaag tagcagacga tttctgtatc agacagttgc tgccgaataa
    25921 attgaaagat caatactgct ttaaaactga ggcagctatt gagtgtctga aatttgtgaa
    25981 aggtgagaag atatggctga agtaacaatt ccaaaggaaa aaatgaatta tacaatcgac
    26041 cttctgatca ctatggtgac agatgaaatc gcagaagaga caggcaagga cagaaacgag
    26101 gtattgacag attttttgtg ttcaaagaca ggaaaagcac tgtatgatga gaagacaaag
    26161 ctgtggtgca ctggtccggc atatatagct gaactgtata tggaagaatt aaagaaatcc
    26221 tgatatggga tgtgagttta aatttatacg acaatggtga caaagacttt cattccaagg
    26281 agcaacaatt aaatatttca aaaacgctga ggtcagcatt tcctaatcac aggacaacga
    26341 cctcagtttt ttatacccag gaatcggcat tcatgaataa gccggttctt tttgtttaca
    26401 gaaaaaagaa ggagtacaag aaaatgagca gagcaaaaaa tccaaagtat gaatccatgt
    26461 cattggagga actgaaggcc aacatggaaa agagcagaca ggaactggag gcagcaattc
    26521 accacaaaaa cattctggag cagcggaaga agctggtaga acgcagggaa cgttcccacc
    26581 ggctgattgt caaaggggca gagtttgaga aagtatttcc gctgtccaga gatctggaac
    26641 aggaagacgt ccaggacatg atgaatcaac tgcagaacag cagttacaac aagagtattg
    26701 tgcggcaggc tcatatagcc gccttacaca aagatcaaca gcagattgca gaagcagtaa
    26761 aaaagacaga gaagggagat gacagttaat ggcactgtat catttttcag taaggaatgt
    26821 atccagaggg aaagggcaga tggtggtcgc ttccgcagcc tatatttccg ggcagaggat
    26881 ttttgacagt tattacaaca agatacacga ttatacgagc aagtcaggtg tgatatttac
    26941 agaaatcctg acaccggagt atgtgccaga gcgtctgact gacagggaaa cactctggaa
    27001 cgaggtggag catgtggaac ggaacaacaa ggcacagctt gcctattcgt ttgacattgc
    27061 actgcagaat gaattgacac ttgaggaaaa tatcagactg gcaaaagagt tctgccagga
    27121 gcagtttgtg gcacgtggca tgatcgtgga tctggcagtc catgagggaa aagcggaaaa
    27181 tgaagaagaa ccggataatc cccatttcca tgtgcttgtc ccaatccgtc cttttacaga
    27241 ggaaggaatc tggggcaata agcagagaag ggaatatatt cttgatgagg atggaaaccg
    27301 ggtgaaggat gccaaaggaa aagatatgtt caatgctgtt tctactaccg gatggaatga
    27361 tccggagctt ttaaaagaat ggcggcgggc atggacagag aaagtaaatg ggaaattcag
    27421 ggagtgccac atggcatcca gaatcgacca ccgttcctac aaagaacagg ggattgacct
    27481 gatcccgacc atccatgagg gatatgaagt ccgggcaatg gagaaaaagg gaatcaaaac
    27541 ggtgatcgga gaattgaacc gggcaatccg gcagtttaac cagatgttta tttctctgaa
    27601 agaatccatt cagtggatga agacagctta tgaggaaatg aaagcagagc tggatcgcag
    27661 gcagaacccg acactgctgg aaagcctgca ggattattat gataaaaaga cacagggcag
    27721 accaccactt cccaattttt atgcggagat gaaacagaga ggtaagaatc tctccaattt
    27781 acaggagttt gcaaaatcca tcagttatct tcagacacat cagatagaaa cgatggatga
    27841 cttgcaggaa cggattgagg aactgaacgg tgttgtttct gtcagtaaaa aagaaatttc
    27901 tgaaaagcgg aaacagttaa aggaacttga gaatctggaa aagatggcag aggtaataaa
    27961 aacaaatcag ccattgattg atgagtataa ccatttctat tttcagaaaa gacgggagag
    28021 atattatcag cagcataaaa aagaaatcaa ttattaccgc aaatgtgaac gggaactgaa
    28081 acaacatctg gataaaaatg gaaaagtgcc aactgccaga tggaaacggg agaaagaaga
    28141 acttcaggcg gggatcgaag agctgaaagc agataatcag ccgtaccagg aggaactaac
    28201 ttttgtgaaa aaggtacaga gctgtgcgga tattgccaga cgtgaccggg aaatggcaga
    28261 agcagatact tccgggcgtt cagaagaaaa aagggagaag caggtgaagt tcccgacatt
    28321 ttacacagtt caggcgaaag agattcttga agaaaacggg aaagcagagc aacagccgtt
    28381 gaaccagtcg gaacagaatc cagaaaagaa gacctctctt ctgagaaaac tggatgaaaa
    28441 aaagaaagag tgtgcagagc gtgatgcaaa acaacaggca gtgaagaaaa agcgaaacta
    28501 tgacatgagt ttataacagt ctgtcagcag gacagacggg aaaggatgag caaaatgcag
    28561 tatacagaag aacaaattat aagagcaaat cagacagatc ttgtttcttt tctgagcgca
    28621 cagggagaac aactagggaa aagcggaaag gaataccgtt ggaaaaagca tgacagtgta
    28681 acggtatccg gaaaccggtg gtacaggcac agtcagggga ggggtggcta tccggtggat
    28741 tttgtgatgg aattttacaa tgccacattt ccggaagctg tcaagatgct aaccggggag
    28801 gaaggcgaag gcaggaacag tacctgcccc gccccgtccc ctgacttccg attaccagag
    28861 aaagaggaaa ataatgatag aatcatcaga tatctgacag aaaacagagg gattgaaaag
    28921 aatatggtgg aggaatggat cggcagtggg gatatttatg aagaaaagaa gcaccataat
    28981 gtggtgtttg ttggcagaga tgcagacggt attccaagat atgcccattg caggggaact
    29041 ggtgaaacaa aataccgggg agatgtggct gaatctgata aatcttacgg attctgtcac
    29101 agagggacgg ataatcagtt gtttgtgttt gaagcagcca ttgatctgct ttcttttatc
    29161 caattatttc ccaaagactg gaaaaagcgg agctacctga gtctgggtgg catttccagt
    29221 gcggcactga tggcttttct ttctgaacgc cctcagatta cttcggtttt cctgtgcctg
    29281 gataatgacc atgcagggaa tgaagccagt gagaaattgg caatagaaat cccggacgga
    29341 tacagtgtca tccgtctgaa accgtcccga aaggactgga atgaaatcct ctgtgataaa
    29401 aatgcagaca ggaagaaatc aatcattgaa acagttacaa tgaaagtacc ggagaaggaa
    29461 gaactggtgc cgatgttgtg ttatgaagac attgaacaga ccagtgtaga atggctgtgg
    29521 tttccctatc tgccatttgg aaaactgacg attattcagg gcaatccagg agagggaaaa
    29581 acttattttg ccatgatgct gaccgcagcc tgcacgaacc gtaagacttt cccgaacatg
    29641 gaagagattg aaccttttaa tgtgatctat cagactgcag aagacggtat gggagatacg
    29701 attaagccga ggctggtgga agcaggagca gacctgagtc gggtgatggt cattgatgat
    29761 acagaggaag cattgacgtt atccgatgac cgtattgaaa aagcaatccg tcagaatcag
    29821 gtcagacttt taatcattga tccggtacag gcttttatcg gtgccgatgt ggatatgaac
    29881 cgggcaaatg aagtccgtcc ggtattccgg aaactgggaa tgatcgctga aaagaccggt
    29941 tgtgcgattg tgctgatcgg acatctgaat aaatcttcag ggacacagag tacttaccgg
    30001 ggacttggtt ctattgatat catggcggcg gtgagaagcc tgctttttat cgggaaagta
    30061 aagaaagacc cgacaacaag agtactgatc catgagaaaa gttctcttgc acctccaggt
    30121 gagacgatgg catttaaact tggagatgaa gaaggattcc gctgggtggg agcttatgag
    30181 atttccgcag atgatcttct ggacggaaag gaaggaaagc cgacagaaac aaaactgcaa
    30241 cggggaacga aactgatcta tgaactgctt gcagacggaa atgcagtcac aatccgggaa
    30301 ctggatgaaa aggcaaaagc acagggaatc tcccagagaa ccatgcgaga agccagaagc
    30361 cggatgaaag aagaactgga ttatcggatg aatgaaaagc aggaaaatac cattcgatta
    30421 aaaaaacaag ggagaatggg tgacggcaga attttggagt gaacggcagg ctttatagat
    30481 accctatctg ccgtctgccg tctgccggtg caaggcaatc aaacggaaat agcaggtatc
    30541 catacagaat aaggattgct gtgaatctgg aacaaaaatg aattgtaata tggcggatat
    30601 aatggtatga ttatccggta acagcaatgt tacggggaag cacctgtgac agggtgtggc
    30661 tgcctcctga tatttacaga ccggagcctt tcggggttct gagatgaaag aaaggaggtg
    30721 cgtgatatga cagcttatga gattatctca atattcttag gcgtactcag tttactgatc
    30781 gcttttggta gcctgttaat tgcgattatt gcctttcttg atcggaagaa caagaggaaa
    30841 taaaaatgcc tatcctgtct gccaacagga taggcggtgg tcgtaagacc atttagtcaa
    30901 ctcaggcggc atccactttg gagtgggtgc ttttcctata acaaatgtac cacacattcc
    30961 tcttgttttc aatgggaaaa ttaaaatatg cagttcatct gacagggatt tatccctgtc
    31021 tttattatat ccccaatttc tgaaatgtta atacaaaaat tttaatatat gtaaccaaca
    31081 gtctgggcag ttaatctgta aaaatggatt agctgctctt tttttgcaaa aaatggaggt
    31141 tttacaatgg cagtgaagaa gaaaattatc aacatcagag tatattttga cggtgatcag
    31201 gacgcaaaag aagtgtttgc agaggtgatc gcacagaaaa tacgtgacac gaaaacaaaa
    31261 gataaacttg caataatgaa agataagcag tataatgatt atgagttcag taatctgaaa
    31321 tctgcgtagg atgtgcaaag taacggaggt atcagatgaa caacagatat atgagtaaca
    31381 atatagaaag aaatgcaacc tggagagttg gtgtatactg ccgactttcc aaagatgacg
    31441 aattgaccgg agagagtgcc agcatctcca accagagaga catactggtg aattactgtc
    31501 agtcacaggg ctggcaggtg gtggatgtgt ttcaggacga tggttataca ggattgaaca
    31561 cagaccgacc tgatttgcag agacttctca aagcctgtga aaaaggactg gtgaatcttg
    31621 taattaccaa agaccagagc cgactgggac gaaaccatgt acagacagga tttctgatgg
    31681 aggagttttt cccaaagcat ggagtaaggt atatcgcatt atatgataat gtggatacct
    31741 ttgccggaga caatgagatt gccccattta agaacgtgct gaacgagatg tattccagag
    31801 atatttccaa gaaagtccat gcgtcctatc acctgcaggc aaccaaggga aagtttacag
    31861 gtgttgttcc accactggga tatcagaaag acccggagga aaagggacat ctcctgattg
    31921 acgacgagac agcaccgatt gtccggaaaa ttttccagtg ggcggtggac ggtcatggga
    31981 ttaactttat atccagagaa ctggaacgcc agcagattcc ctgcccgtca tggtggcacc
    32041 ggaaacgggg actccgcacc tattacacaa aatgggaaaa gaaagacccg gaaaatggga
    32101 aatatgtatg ggatgaatcc tatctgaaat ccctgctgat gaatcccgtt tactgtggtg
    32161 acatggcttc ccagaaaagg tactaccgtt tcaagattgg caatcaggga gataaagctc
    32221 cggaggactg gatcacagtc agggataccc acgaagcaat cattccgcag gatgtatttg
    32281 atctggtaca ggcaaagatg aaaggcagga aacgccagac agaacaggga gaatacagca
    32341 tgtttgccgg attactccgc tgtgcagagt gtggcggtgc gttgacgttg aagaagacgc
    32401 acaccaaaga ccagcatgaa gtttatacct gttccactta tatccataag gggaaagcac
    32461 attgtaccca gcaccgggtg gacgctgatg acctgtatga tgcagtcctt accagaatcc
    32521 aggaatgtgc aaaagcagtt accggagacg gaacagaact ggaaaaccgg gtaaaggagt
    32581 tatgcgagga agatacacag ggacacaggg aaagtctgga gaagctggta tcaaagcaga
    32641 aagaccgcct ggaaacgtta gaccggctga ttgcaaaact gtatgatgac cttatcaatg
    32701 acaagattac agaatctgtt tttgataaga tgctggagaa gacacagaag gagcagacag
    32761 atatcaaaaa ggaacttttc cagaatgaga gtgtgctgaa tacggaagaa aaactggatg
    32821 cacagtccca gcagtggatt gacgatatta gcgagtatgc ggacatcaaa gaactggatg
    32881 ccaatcttct gaaccggctg atcagtaaaa tcgttatctc agaaccgcag gagaaagacg
    32941 gaacagaaaa ttactgccgt actcctgaaa cggtcacaat ggaaattcat ttcaacttaa
    33001 aaccaatacc ggagcttgga accatcgaaa gaggttctgg ctcccataaa taagaaaaat
    33061 ctatcaaaat ttaatctatt ttaatctttt caaggctcat ttcgagcaaa tattttctca
    33121 taaaagacat aaaacagctt gtcgctggcg gcggcgtggc cctgatcggt gtcactctgg
    33181 ttcccctgct gtccgggctg ctgggctgat cctggccttt gtccctgtcc aaacccttaa
    33241 ctaatcgcct ccgcgtcctc ccggccagac cgggagggcg cgggaaagga ggtgctaccc
    33301 gtgattagtg aaatcattga agaatggata aaaggcatcc tcatggacgg tatcacgggc
    33361 aacctgtccg ggctgttcga tacggtaaat gccaaagtcg gagaaatcgc cgccgacgtg
    33421 ggacgaaccc cgcaggcatg gaacagcggt attttcaata tgctgcacag cctgtcggaa
    33481 accgtgatcg tccccatagc cggggccata ctcgcccttg tcatgtgcta tgagcttatc
    33541 cagatgataa cggaaaagaa caatatgcac gactttgaca gctccatgtt cttccgctgg
    33601 atattcaaat ccgcgtttgc aatcctgatc gtaacgaaca catggaacat cgtcatgggc
    33661 gtattcgacg ccacccaaag cgtcgtcaat caaagcgccg gtgtgattat cgggcagaca
    33721 tccattgatt ttaacaccct gataccagac cttgaaagcc aattggaggc tatgagcgtg
    33781 ggcgctcttt tggggctgtg gttccaaacc cttgttgtgg gcctcaccat gaatatcctt
    33841 tccatctgca ttttccttgt gacctatggc cgcatggtgg aaatctatgt tgtcacgtcg
    33901 ctggggccta tcccccttgc cacaatgggc aacgcggaat ggcgcggcat gggccaaggg
    33961 tatctgaaat ccctgctggc gctgggcttc caagcctttt taatcatggt tgtggtgggt
    34021 atctacgcgg tgctgataca gaatatcggt tccgcttccg acatatccgg cgcaatctgg
    34081 ggctgtatgg gctatacggt gctgctgtgc ttctgcctgt tcaagaccgg cagtatatca
    34141 aaggccgtat tcggggcgca ttgatcccaa aatccaagca tacacaggag gaacttgttt
    34201 atttcaacgt acaacatcat ttacgctgac ccgccgtggc ggtatgactg caaaaacgta
    34261 cagggcgcag cggaaaatca ctattccacc atgagcattg acgagctgtg cgccctgccg
    34321 gtggaacggc tggcgtccaa agactgcctg ctgtttttgt gggccacatt ccccatgctg
    34381 ccggaagctc tgcggttaat caaagcgtgg ggcttttcat tcaagaccgt ggcttttgta
    34441 tggctgaaac agaaccgtaa aagccttaca tggttttacg ggctgggccg ctggacaagg
    34501 ggaaacgcgg aaatctgcct gctggcaaca cacggcaagc cgaagcgtcg ttctgcctct
    34561 gtccatcagt ttattatcag cccgattgaa caacacagca aaaagccgga tgtgacccgt
    34621 gaaaaaatcg tagagctggc gggcgatttg ccccgcgtgg agctgtttgc cagacagaaa
    34681 acccccggct gggacgtgtg ggggaacgag gtggaatgtg accttatcct gacgtgaaag
    34741 gaggtgccaa atggcttatg taaccgtccc caaggactta acccatgtga aaagcaaggt
    34801 tgtgtttggg cttaccaaac ggcaactgat ctgtttttcc ggggcgctgg tgatcggcgt
    34861 ccccttatat tttctcaccc gtgactatct taccaacagc gcggcggccc tgctcatggt
    34921 atttgccatg ctgccgggct ttttgcttgc catgtacgag cgtcacggcc agccccttga
    34981 agtggtgatt atgcagatca tccagtgctg cttcatccag ccaaaggagc gtccctatca
    35041 gaccgacaac acctacaccg cccttgtgcg gcaataccag atggaacagg aggtaaaggc
    35101 cattgtccaa aaaggaaatc ccaagagaaa ccgaaaaaac aaagctgacc cgcgcccagc
    35161 gaaaagaaat tgacgcggta atccgtaaat ataagggcga cggcaggccc cacacggcgc
    35221 agcagagcat cccctacgag gtcatgtacc cggacggcgt gtgccgggta tcgcccggcg
    35281 tcttttccaa gtgcattgag tttgcggaca tcagctatca gcttgcccag ccggacacgc
    35341 agacggctat ctttgaaaaa ctgtgcgacc tgtataacta tgtggacgcc tccattcata
    35401 tccagttttc tttcctcaat cggaaagttg acccggtgca gtacgccaag agctttgaga
    35461 tcgcgccgca gggggacgac ttcgacgaca tccgggccga gtacaccggc attctgcaaa
    35521 agcagcttgc aaacggcaac aacggcatgg taaagaccaa atacctgaca ttcaccatcg
    35581 aggccgaaag cgtgaaagcg gcgcgagcca gattgaagcg catcggcttt gacctgttag
    35641 gctatttcaa gagcatgggg gccgtagccc atgtgatgga cggctgggaa cggctgaacc
    35701 tgctgcatgg cgtgtaccac ccggacggcg agatattcaa ttttgactgg aagtggctgg
    35761 ccccgtccgg cctctccacc aaggatttta ttgccccgtc ctccctgtgc ttcggcaatg
    35821 caaagacatt cggcatgggc ggcaaatacg gcgcggtaag tttcctgcaa atcctgtccc
    35881 cggagctgtc ggacgatatg ctggccgact tcctcaatac ggaaagcggt gttttagtca
    35941 atctccatgt gcaggccatt gaacagacca aggcgatcaa gaccatcaag cgcaaaatta
    36001 ccgaccttga cgctatgaag attgcggagc agaaaaaggc cgtccgcagc ggttacgata
    36061 tggacattct tcccagcgac cttgccacct acggcgagga cgccaagaag ctgctgacaa
    36121 agttgcagac ccgcaatgaa cggctgttcc agttgacgtt ccttgtcctc aacgtggccg
    36181 acaccaagca aaagctgaat aacgacgtgt tccaagccgc aggcgtggcc caaaagcata
    36241 actgtcccct tgtccggctg gactaccagc aggaacaggg ccttgcaagc agcctgccgc
    36301 tgggcgtcaa tcagatcaag atacagcgca gccttacgac ttccagcgtg gccgtgttcg
    36361 tcccctttgt gacgcaggag ctttttcagg gcggcgcggc catgtattac ggcatcaacg
    36421 ccaagtcccg caacatgatt atgctggaca ggaagcaggc ccgttgcccc aatgccttga
    36481 agctcggcac ccccggcagc ggcaaatcca tgagctgtaa atccgagatc gtcagtgtgt
    36541 tcttaacgac gcctgacgat atttttattt cagacccgga ggccgaatat tacccccttg
    36601 tcaaacggct ccacggacag gtaatccggc tgtcgcctac cagcaaggac tttgtgaacc
    36661 cgctggacat caacctgaat tattcggagg acgataaccc gctggccctg aaatccgact
    36721 ttgtgctgtc gttctgtgag cttgtcatgg gcggcaagaa cgggctggaa gccattgaaa
    36781 agaccgtgat agaccgggct gtgcgcgtga tctaccggcc ctatctggca gacccccgcc
    36841 cggagaatat gccgatcctg tccgacctcc acaaagcgtt gttagatcag catgtcccgg
    36901 aggctgaccg ggtggcgcag gcccttgacc tgtatgtgtc cggctccctg aacgtattta
    36961 accaccgtac caacgtggac atcggcaacc ggcttgtgtc ctttgacatt aaggagctgg
    37021 gcaagcagct caaaaagctg gggatgctca ttgtccaaga ccagatatgg gggcgcgtca
    37081 cggccaaccg aagccaagga aaggccacat ggtattttgc cgacgagttc catttgcttt
    37141 tgaaagagga acagacggcg gcctattctg cggagatttg gaagcggttt aggaaatggg
    37201 gcggcattcc aacaggtgcc acccagaacg tcaaagacct gctttcctcc ccggaaattg
    37261 aaaatatcct tgaaaacagc gacttcatta cgctgctcaa tcaggcgtcc ggggatagaa
    37321 aaatccttgc ggaacgcttg aatttgtcca ccgaacagca gaagtacatc gacaattccg
    37381 aacccggcga gggccttttg atctttgaaa acgtggtgct gccctttacc aaccccatcc
    37441 cgcacaacac ccagctttac aagattatga cgacccgctt aaacgaggtg gcgggagtat
    37501 gacccggcga cgccacaaag gaggtgaacc accatgaaag agctgaaagc caaggcgaaa
    37561 gtgacgcagg ccatgacccg cgacgggctg acgctggaaa atcaggccac cggcgaggtg
    37621 gagaacattt ccagccggga ggccgaacag gatttgtccg cgccggggcc gggcggcacc
    37681 gccgataagc tgctggaacg ggccgacgcc atccatgacg gccataaggc caagaaagcc
    37741 gcgaagcagg ccggggctgt ggccgccgag ggggacgctg ccctgcaccg gccatccgcc
    37801 cggctccaac tgtcggagga agaacgggcc gacccggatt tacagcccta tatccgaaaa
    37861 tcggacaaac gggccgataa gctggataag gcccgcgccg ccatccccaa gaagcgcgtc
    37921 ccggtcaagg aaaccgtctt tgacgaggca agcggcaaag caaagaccgt cctgcacttt
    37981 gacaaacagg acaaggggcc tccctccctg aaacccaatc cggcaggccg tcctgtttcc
    38041 gaggtttttc tattcggcca cgggaaaatc catgaagtcg agcatgaaaa cgtgggtgtg
    38101 gagggcggcc ataaggggga ggaacttgcc gagcggcagg ccggaaaagc catccgcagc
    38161 accatccggc accacaggct gaaaccctac tgggacgcgg aaaaggccga gcgcaggcta
    38221 ttgtctgcca acgcggagta tttctaccag aaatctttgc gggataaccc ggagatcgca
    38281 caggcggcca gcaaccccat ttcccggttc tggcagaaac agcagatcaa gcgccagtac
    38341 gccaaggccg cccggacagc gggccagacc gcaggcgcgg cggcacaggg cgcggccaag
    38401 acagccgggg cctccacgaa agcggccaag gccgcagcca aagaaagtga acgggcggcc
    38461 tcctttgtgg cccgccactg gaaaggggcg ctggccgtcc ttgtgttggg gcttttgatg
    38521 ctgcttatca tcggcggctt acagtcctgc accgccatgt tcggcagcgc cggaagcggc
    38581 atcacggcgt cctcttactt ttccgaggat agcgatatgt tggccgccga ggacgcctac
    38641 gcggagctgg aagccgagct gcaagactat ctggacaact acgaggccac ccatgactat
    38701 gacgaatacc attttgacct tgacgagatc agccatgacc cctatgtgct gatttccatt
    38761 ctgtcggcgc tccatgacgg gatatttacc atcggggagg tacagggtga tttgtccatg
    38821 ctctttgaca agcaatatat cctgacagaa aacgtcgtgg tagaaacccg ttaccgcacc
    38881 gaaaccgata catggacgga cgcggagggc aacacccata cagacaccta ccaagtgccg
    38941 tatgactatt acatctgcta cgtcacgctg gaaaacttcg acctgtccca tgtgccagtc
    39001 tatatcatgg acgaggaaac gctaagtttc tattccctgt atatgtccac gctaggaaac
    39061 cggcctgatc tgttttccgg cagccagtac cccaacgcct ccaccctgac gccgccgacc
    39121 tactacgacg tacccccgga ggctttggag gatgccacct ttgccgccat gctcaaagag
    39181 gcggaaaaat atatcggtta tccctatgtg tggggcggca gctctcccag caccagcttt
    39241 gattgcagcg gcttcatttc gtgggtaatc aatcatagtg gatggaacgt gggacggctg
    39301 ggggcgcagg gcctatataa catctgcacc cctgtttcca gttcgcaggc caagcccggc
    39361 gaccttgtat ttttcaaggg aacctacgac acccccggcg tgtcccacgt cggcctgtat
    39421 gtgggcaaca atgtgatgct gcattgcggg aacccgattt cctacacgaa ccttacttca
    39481 aactactggc aatcccattt ttacagttac gggcgtttac cttaacgcca gaaaggagct
    39541 ttcaattatg gcaaccaaac ttgaccgcat cgaaaaggac atccagaaaa ccaagtctaa
    39601 gatcgcggag tttcagaaac agcttcggga gctggaaacg cagaaaaccg aacaggagaa
    39661 tttgcagatc atccagcttg tgcgcggcat gaacatgaag ccggaggaat ttgccgcgtt
    39721 cttacgcagc ggcgcaatgc aggccgcccc tgccgcaacc ccttaccatg aacaggagga
    39781 caatgcccat gaagaataag accagacgga tttcccgtat tctccccgca cttctggccg
    39841 tgatgctctg cgtcacggcc tttcccgtta ccgcccttgc aggcggcaat gacccggcac
    39901 cggccccgct gccggaggct acaaccgaac ccgctacggg cggtttggag cctgaaacag
    39961 acgaaaccgg caccgccacg gacgggacag aaaccgaggc cgaaaaagat accggccctc
    40021 tgaccggcaa ggacatcacc gacctgtttt ccgcgctgtt cggttccaag gtgagcattg
    40081 ccgccacgga cgacggcatc cagatcacca ccggcaccga caaaaaagaa cctgaacaga
    40141 ccggcaccgt caccaccaac ggcggcaggc tgaatgtccg caccggcgcg gggcttgaca
    40201 agaccgcgtt cacccagctt cccaatggga cgacggttga ggtagtcggc acagacggcg
    40261 actggattaa aattctcttg ccggagcgca tcggctatgt ccattccgac tatatgaccg
    40321 tcagcgaaaa ggaagtagcg gctaccggcg agggcggttt ttccctctcc attgacccgg
    40381 aggaaattgc ttctctgctg gaactgttca atggcggcgg ggccagcgcc gccctgtccc
    40441 cggacgggaa cctgtcactg attgacgaca tcggcagcgc cgcgaaaaat ggcaagcagt
    40501 ttatcaccgt ggaaaccaaa aacggcaatg tgttttatct gattattgac cgggacgacg
    40561 agggaaaaga aactgtccat ttcttaaatc aggtggatga agccgatctg ctgacgctga
    40621 tggaggacgg ggaaaccgtc acccccgctg ctgtctgctc ctgtaccacg aaatgcaagg
    40681 ccggggctgt gaacacgaat tgcccggtat gtaaaaccaa cctgaccgag tgcagcggcc
    40741 cggagccgca ggaaccccag ccggaggaac ccgaagcccc ggaggaagaa cccaagggcg
    40801 gcgcgggcgg cctgatcgtg ttcctgctgg tggcgctggc gggcggcggt gcggcccttt
    40861 actatttcaa gttcaaaaag cccaaggctg aaaccaccgg cagcgacgat ttgggcgagt
    40921 atgattttgg ggaggacgag gatttggagg acgaggaacc cgaaaccgag tttgacccgg
    40981 aagctgattt tgtgagtgaa caggaggacg aggaatgaca tataaacttg taatcgcgga
    41041 aaaacccagc gtggcccgca gcattgcggg cgtgatcggg gccacggaga aacacgacgg
    41101 ctatttggag gggaacggct atctggtgag ctggtgcatc ggacatctgg tgtcctttgc
    41161 cgacgccgga cgctatgacg agcgtttcaa aaaatggcgc tatgaggatt tgcccattct
    41221 cccggaaaca tggcagtaca tcgtccccga cgacaagaaa cagcagtttg acgttctctg
    41281 ttcgctgatg cagcgccccg acgtgaccgg ccttgtgtgc gccaccgacg ccgggcgcga
    41341 gggggaattg attttccgtt tcgtctacca gatggcggga tgccaaaagc cctttgagcg
    41401 cctttggatt tcctctatgg aggacagtgc tattaaggac ggctttgccc acctgaaacc
    41461 gggcggcgac tatgatccgc tgtatcaatc ggccctgtgc cgggccaagg ccgactggct
    41521 gatcggcatt aacgccaccc ggcttttttc cgtcctgtac cacaagaacc tgactgtggg
    41581 ccgcgtccag acgccgacct taaaaatgct ggtggacagg gacgccaaga ttaccgattt
    41641 caagaaagag aaatatcaca tcgtccatat cacggcgggc ggcgcggacg cggtaagcag
    41701 ccgcttttcc gacgctgccg aggcaaacac cgtcaaggcg gcttgtgcaa aagcgcaggc
    41761 cgtttgcgtt tccgtgacgc gggagaaaaa gacggaacag ccgcccaagc tctatgacct
    41821 taccaccttg cagcgggagg ctaaccggct tttcggctat accgccaagc agacccttga
    41881 ctatgcccag gcactgtatc taaagaagct gctgacctat ccgagaacgg atagtcaata
    41941 tcttaccgac gatatgctgc ccgccgtgga aagccttgtg tccggcctgt gggagctggt
    42001 gccgtttgcc aagggcctga acctttcccc gcagtttgac cgcatcctga acagcaaaaa
    42061 ggtgtccgac caccacgcca ttatccccac cgctgaattt gtgaagcagg gctttggcac
    42121 tctggccgag agtgaaaaaa agctgctgtc cctgatctgc tgcaagctct tatgtgccgt
    42181 ggccgggccg catatctatg aggccgtcac cgccacattt acctgtgccg ggcaggagtt
    42241 taccgccaag ggcaagaccg tcctttcccc cggctggaaa gagattgaac gccggttccg
    42301 ttcttcctta aagaccgacg ccgacgagga cgccgaggcg gtgcgggagc tgccggagct
    42361 ttgcgagggc cagacctttg cagacgtggc cgcctctgtc accgagcatt tcaccacgcc
    42421 gcccaagccc tacaccgaag atacgctttt gtccgctatg gaacgggccg gggccgagga
    42481 tatgccggag gatgccgagc gcaagggatt aggcacaccg gccacccgcg ccgccattct
    42541 ggaaaagctg gtgcagatgg gctttgtcca gagaaaaggc aagcagctca ttcccaccaa
    42601 ggacggcatc aatctggctg tggtgctgcc ggaggcgctt acttctcccc agcttactgc
    42661 cgaatgggaa aaccggctga ccgagatcgc caaggggcag gccgatcctg acgaatttat
    42721 ggacggcatc gagacgcagg cgcgggagct ggtgcagacc tattcctgta tcagtgagga
    42781 aaagcagaaa ttgtttcagg ccgagcgtgt tcctatcggg acgtgtcccc gctgtggtga
    42841 ggctgtctat gagggcaaga aaaactacta ctgcggaaac cgggcctgcc agttcgtcat
    42901 gtggaagaat gaccgatttt ttgaggaacg caagaaagcg ttcaccccga agatcgccgc
    42961 tgcgctgctc aaaaccggca aagtcaaggt aaagggcctc tattccgtaa agaccggcaa
    43021 aacctacgac gggacggtgc ttttggctga taccgggggc aagtacgtca actaccgcat
    43081 tgaacacagg agctaaagga cagtaacaca gcaagcatag ggagtgtggc tacactcccg
    43141 gtaaaatccc ccggccgcgt ccctgccggg ccggggattt ttgcttgttg gggaagtccc
    43201 caacccccta ttttttgaaa cagaacagag aaaggagtga ccgcgtgaac gaagtatttt
    43261 ctatccaagt ccataaccgg cagtttttgg aacagggcaa acaaggcgtc tggctggatt
    43321 tgcccaccac aaccgaaacg ctgcaagccg ccctgcggga tgttggcatt actgccgaca
    43381 atccgcagga tttttttatc aacgggtatt cctgcccgga ggacagacac cttgccattc
    43441 cctatgacat ggtgctggcc gccgacgtgg acgagctgaa tttccttgcc gcccggctgg
    43501 gccagcttga cgccgccgag atcgcagagc tgaacgccgc cctgcaaaat ccccgtggcg
    43561 gctttgagaa tatcggccag atcatcgact acaccgaaaa tgtggattac tatgtgcatc
    43621 ttcccgacgt taggggggat ggacagttgg gcgactacta cctgaaccgt tccggcatgg
    43681 tggatatgcc ggaggaatgg aaagccggga ttgatacggc gcagtttgga cgccacattg
    43741 cccagcagga acaaggcgtt tttacccagt acggctatct ggtgagaagc ggcgacgaat
    43801 ggcagcgcgt ccatgagggc cagccggtgc cggaggaata ccgggtgatg cactaccccc
    43861 agccggaagc tgaccgggag gaaccgcagg cccagcagcc ggaggcatcc gaaagccccg
    43921 acaaagcgcc gcccccggtt atcccgatca tgctggacgg catgaacagc aaagaacgca
    43981 tgaaagtgat tactgaccgt ctggaaaccg gcgtacagga gctttttgaa agtagccgct
    44041 atatggctta cttatccgct atgtccaaga tgcacgatta cagtctgaac aatacgctgc
    44101 tgatcgccat gcagggcggt tcgattgtgg cgggctataa caggtggcag gatgaatttg
    44161 accgccatgt gaagcggggc gagaaaggca ttaagatact ggccccggcc ccgttcaagg
    44221 ttaagcagca gatggagaaa atcgacccgg ccacccagca gcccgtgatc ggcgcggacg
    44281 gcaagcccgt gaccgaagaa caggaagtga cggttccggc ctttaaggtg gtgacagtct
    44341 ttgacatcag ccagaccgag ggcaaggatt ttcccatgcc ctatatcagt gaacttaccg
    44401 gcgacgtgca gcagtatcag gatttctttg ccgcgctgga acggacatca ccctatccca
    44461 taagcattga gccgatttca gacggggcca agggccgctg cttctacgca aaacagcgca
    44521 ttgccatcaa cgagggcatg agcgaattgc agaccctaaa gaccgccatc catgagattg
    44581 cccatgccaa gctccacgcc attgaccggg aggcccccgc cgaggaacag gcggcccggc
    44641 cagaccgcag cacccgcgag gtacaggccg aaagcgtggc ctataccgtc tgccagtatt
    44701 acgggctgga cacgtcggac tattcttttg gctatgtggc cgattggagc agcggcaggg
    44761 agttatccga gctgaaagcc tcccttgaaa ccatccgggc cacggccagc gaattgataa
    44821 ccgagatcga cggccatttt gcccagcttc agcaggagcg cgaggcccgg caggaacagg
    44881 aggccgtcac ggttgacgtt accccggagg cagcggcccc ggcgcaggcc gaggaagaac
    44941 aggccccggc ccttgatccg gcagccgagc cgcttgtgac tgtgctatgg agcgaaagcc
    45001 cccagctaaa ggacggccag caaatgccgt tgcatgaggc cgacgcgctg tttgcccgtc
    45061 tggatgcagc atatccggca gaggcaggct atgacaaaac cgcgttccgc attgactgta
    45121 tctttcaggg cgagccggac agctacgagg ggcggcagga ttttggagat agggacggtt
    45181 ccctgatcga acatatccag agctaccacc aattttacgc gcaggacgaa aactggaaaa
    45241 agcatgtaat cgctacaacc ggcatagagg gctgggagca ggacaaggcc catcgggata
    45301 tgctgttagg cgagtttgtc ccctatatga agctgcattg cagccttgcc caaatggaac
    45361 agacggcgca gggccgctta cagtccgggg aaaccctgac gccggaggaa acggcctatt
    45421 tgggggcgct tctctcttat gtgcaggagt gccgccccat gctcaatcag ggccagtatc
    45481 agttgccgga gccgccgaaa ctggctgact tcgaccagag cttgcaggac tacaaagcac
    45541 aggtacaggc cgagatcgcg caggaggccg ccgcagccgg tatgaccgtg gaggaatacg
    45601 cggcggctgg ctatgaagca ccccaacagg acaccttttc catctaccag cttcgaggcg
    45661 gggaggaaac ccgcgactat cggtttgagc catacgagcg tttacaggcc gcagggctgg
    45721 ccgtggacag tgccaactat gaccttgttt atacggcacc gctggataaa agcacgtctt
    45781 tggaggatat ttaccgcaca ttcaacatcg accacccggc agattttagg ggacacagcc
    45841 tttccgtttc cgacgtggtg gtgctgcacc agaatggaca ggacaccgcc cactatgtag
    45901 acagcttcgg ctatatggaa gtgccggagt ttttgcggga aaatcccctg cgtaccgccg
    45961 aactgtctac ggagcagaac gaaaacatga ttgacggcat tatcaacaat acgccgcccg
    46021 aaccgcagcc ggaggaaccc gaacagctat cttttgagga tgtggcagtt gcttccggcc
    46081 agacacagga ggaacccccg gaggctgcgc ccccgcaaac ggctgtccgc tattatccca
    46141 tcaatgaggg agccgcccgc cgcgccaagg aagccatgag cttttctgac taccggcccg
    46201 gcagcgccac ggcggaatac cggcactatg tcgatcaggc cgtggaaatt gcggagcgcc
    46261 agaaaaagcg tgtggagcca gagtatcacg aaaagataga cagcctcctt gacacctacg
    46321 cccgcaagct ggccgagaac atgaataagg gctatgagat taccgcccgc gtcccctctg
    46381 tcctgattgc cgggccgtcc aatttcccgg tacgggcgaa agaaaagcag aacgccgcca
    46441 gtgaccgcaa catggaggaa tttcagtaca ttcagggctt gcttgacaaa atccgcagca
    46501 ccggcatggg tggtatcagc gccgacgatc cgcaggccgt ttccaagctg gaaaagaaac
    46561 tggaaaagct ggaagcgtcg caggagttga tgaaggccgt caatgcctac taccgcaagc
    46621 atgggacgct ggacggatgc ccccatctga ccgaaagagg cattgaaaac ctgaaagcgg
    46681 atatggcgtc gggctggcac tatgagaaaa aaccgttcca gtcctggcag ctctccaaca
    46741 ataacgccga gatacgccgg ttaaagggcc gcattgagga attgacccgc caaaaggagg
    46801 cggcctatgt gggatgggag tttgacggcg gcactgtgga gatcaaccgg gtggccaacc
    46861 gcctgcaaat cttctttgag gggaagccgg atgctgctgt acgcgacgag ctgaaaagca
    46921 acggtttccg ctggtcgccc aaggccgggg catggcagcg gcagttaaac gacaccacaa
    46981 tccgcgtcgc tgaccgtatc aagtgcatcc agcccctttc cggcgaaaag cccagcgcct
    47041 tacagcttgc ggcccgcagg gagaaagaaa agccctctat ccgggcgcag ctcaacgcgg
    47101 ccaagaccga acaggccaag cagccaccgg cgcgggaaaa atcaaaggga ttggaggtag
    47161 agtaaatgca gaagtttgaa aatgtggacg ttctggccgc gctggaacag atcatgcggc
    47221 agaacaccgc gttttatcaa agtgactttg gcattgacaa agacattatc aggcgggatg
    47281 ccgccagcga tcaggccgca gacaagaccc tgctctggat gtcccggccc tccgggacgt
    47341 actgtttccg ggaacgtgat gttttcctga aagacacccg acagtacaac acatggaaat
    47401 tttacgggga gcagacccgt gacaagatac tggcctacgc ggtggagctg accggcacac
    47461 agggcggcac tatccggggc aatctctatg agctggacta tccgcagcat ttccggcacg
    47521 tcattgaaac cgcccagccg gtgagtgtga accggctctt ttatgagcat ggcacccgtg
    47581 acatcccgga gagccagcgt tttgacggaa gccctgaccg ggtattggga aactttttgt
    47641 actatgaggc ccagccccat gacccggctg tgctgcagga ggcgctacgg acggagcagc
    47701 acagccgggg gcgggccgtc cccggcgatt tcaaagtcca cgtcaccgct ttgcatgaca
    47761 gccggattga aaccgaggcc cggcgtattg tggacagcct gaaagatctg tccgcgccta
    47821 acagccccaa taaaacgcat ttcatggtgg agatctcccc gcactttgcg gcgattgctt
    47881 ccaccaagga taatgaccgg ctctttgcca tgctgcccta taaatccctg tgttttaccg
    47941 ggatgaaagg caggcatggc gtgtatgccg tgattaaccg ggacgagcgc cgggatgtga
    48001 gtattcgcag gccccggccc tctatccgca agcagctatc cgacagcaaa caggccgcca
    48061 gcccaaaaaa ggcggcggcc agaaccaaaa agaatgaatt ggaggtataa ggaatgatgc
    48121 actttaccgt ggaggaagaa aacctgatct gtatgtacca caatgcagac cggcgcagaa
    48181 cgataaacag gctcaccgcc gtcatgccgg ttatggatgg ggatatgcgg gcgcaggccc
    48241 agcagaccct ttccaagctg gaacgcatga cggatgccga ctatgacggc cagaagttcc
    48301 acttcacaga cgaataacga acagcagacg ccgggcgcgg cggccatgtg gtcgcttgcg
    48361 tccggcgtct tttctttttg cctattccta cgcatttaga acccggtatt gtcggggata
    48421 ggatgaagta ttcgcttgcc cctgcaaaca tgcgggagaa gccttgcagc gggcggtttt
    48481 caatccctta aatgcgtagg ttgcagctat aattagaccc ctgaaacaga tatagccgcg
    48541 cagcgggtgt atctgtttca ggggtgtgca ggtgatagcc gcaaagcggc gtaagagggt
    48601 gcagccctct tgccgtctat ctctgttcca gcgggaagct cggcaatgct tccagcagtt
    48661 tcagggaaat gcgctgccgt atatcttcgt caatgacttt cttctttttg ccgtttgcct
    48721 gtctgatctc caccgtggaa agctcgttga tgtagtcagc ataatgggcc aacacttttt
    48781 ccgtggccca cctctcaccg gcaacggcgg ctttgatcgt ttcatagtcc agcagctttt
    48841 cttgtccgtc catcctgata cccccgtcag actgattttt tctatgatac catattcgga
    48901 agcatggaac cagagaaaac ggcgcttttc tgctggtaaa ttctacgcat ttagaacagc
    48961 acttcggcgg ggataggata aaacacccac ataccccgct gaccatgccg gaaaagcctt
    49021 gaaatataag ggcttttaca gctctaagtg cgtaggtttc cagtatcttt tccgctggcg
    49081 ctcggcctct ttgcggcggc gcatccgttt ggagcaggcc gcgcagtatt tggcccggtt
    49141 agacttcggc gtgaacgtgg ccccgcactc acagcagcgt ttcacttcgt cccggtcttt
    49201 ggtgatctcg gcatacaggg ccgcatccag cgggaggaca gcggccttaa accagcggca
    49261 cagaagcgaa taggaaatgc tctgtacgca aatacattct tcgccgtcgt ccagcaacag
    49321 gcagttgccg tcacaatagt tgcagcactc atgcgtcagc ttccgggcct tgcgatattg
    49381 agggtaattc attcgtggaa tactcatagc tcttgcgtcc attcctttcc cggcgtcata
    49441 cgcaaaatgc cgtccacatt ctgcttgatg atgccgtatt cctgcatctg ctttttcgct
    49501 tcgccgtact tctcatacag ccgttccttt tcctcggcca atcgcttgta ctcggctttc
    49561 agggcgtaca gatcggggag ctttttcaca ccctgtccct ttagggcctt tgccgcagct
    49621 tcatacaaaa tcaacgtgct ttcatgctca cgccggaatt ttgccttatc cgctgcattg
    49681 cggtattcca tagccacggg ccagagctgc ttgtaggtag aaatattctt tatcagcacc
    49741 gccatatccg caagccggtg ttccatagct ttgagggtag cggcggcctc gtcgctggcg
    49801 gcggtgatct cggccacttt gctttccaaa tccgggtagg tgtctatccc atgttcagtc
    49861 aggaaattca aagtctttgc ggcctgcttc aaattgtgga gcttcgccca cctctcatag
    49921 cctgccgact gctgggcctt aatgttgttt tccagatcaa tccgcaaaga aatccccttg
    49981 cgctctttgg gcgctctggt gataacgcgg cctttgatac gctctttgat ggcttcctcg
    50041 gtgtaggctt cccccaatgt cttgcagcgg gtaaagcgtt cctgtccggg agcgcggaaa
    50101 gaaataaact ttcccggctt gatctcatag ccctgcgcct gcatgagccg caggaaatca
    50161 tcaaaatctt ttgcctgcgg gatagcggcg tcaatggctg ctttgagctt cgctttccag
    50221 ctcgtccctt tccgctgggc gtcccactcg gcatagctct tgcccttact ctggccgggc
    50281 ttcaccacgg acaatccgtt ttccttgcac agcctgtcgc tggtgcgccg gatataggca
    50341 tagctctgtt tgttggaaat gtacttgcgc tgatttacca tatccacggc acagaaaata
    50401 atgtgattgt ggacgtggcc cttatcaata tgggtagtca gcacataggg atatttccct
    50461 tgcagcactt cctcggcaag ctgcttcccg atctcatggg cctgttccgg cgtggcttcg
    50521 ccgggggcaa aggattgtat caggtggtgg cccagattat tgcccttttg gtatgcctgc
    50581 gccagcagca tttcaaattc aatgtccgct gtttcataag agcaggcaaa ggaggccacc
    50641 agcagcttgc cgtcagtttt gtccgggttc tcgatgtagt caagggcttt tttcagcgtt
    50701 gacttgatag gatgtatctt tgtaactgcc atatctgcgc cagcgccccc tttatatcct
    50761 cgatgtcctg tgcgtacacc ggccccatac cgttcagcct ctttgcaatc tgattgacgt
    50821 tgacgccgat cttctgtatc tcggcggtct gcgcccggat gtcgctggtg tccatgttga
    50881 cgatatagcc gtcaatgagc atcttccggg cgtaggctga aaagttcttc gtatgcagca
    50941 gcgccatttt ctgttcaatc atagcccgtt cctccggcgt cacagggacg cgcagcacga
    51001 tttttcgctt tctgtttgcc atgcttcacc gtcctttcca ttcaggggtt tggggatttc
    51061 cccaacaagc aaaacggacg cggcccggca gggacggggc aggccgtttt tccgggagtg
    51121 gctacactcc ctatgcttgc tacgttactg tttctccact aacaagacgt ttccaaagcc
    51181 ccaaactacc cgcaaaatag ccctctggaa agaaaaaagt gcaaaaaaat agaagctacg
    51241 ctgtgcagct cccgctaaaa gtaaaacaga ggatgccagt tacacatcct ccgtttcatt
    51301 cttcttcggc ttttcaatta gtatggcgct gtcaatcgcg ccctcgatga tcggcaggta
    51361 ttcttcgggg cagagtttca gcttgtggct gaccctttgc cgcagttcgc tttcctcccg
    51421 catgatctcc gggttaaagt agcgttccac aggaagcccg caggctttaa taagctgtat
    51481 gacaaccggc agactgggaa tggccccgtc gttttcgatg ttggcaagat agcgccagtc
    51541 aatatttatt ttctctgcca ttgctttgcg cgaaacgcgc tttgcttgcc gggccgcttt
    51601 cacatccgcg ccgaaagttt caaagccggg acaatcttca atattcgcca tatcgcacct
    51661 cccacttaca ttgtatattt catactctgc ccgtggaatg ttggtatatg cacctttctt
    51721 aaagtttata tttcacattt tgtttctttt attcttcgag caaaagaatt ataatgtacc
    51781 ctgtggaggt gtactatgga ctacatgacg ttgaaagagg caggcgagaa atggggtgtg
    51841 cccccacgat ggataaacta ctattgtgct gcgggacgca tccccggcgc tgtaaagatg
    51901 gcgacgatct ggctcattcc caaaactgcg gaaaagccga ttgacaggcg tacaaagaaa
    51961 ggaaaggcgg ttactgaata gtataatgaa accggcgcat tttggatatg gttttgaaga
    52021 gagagaaagc atggtatttt gtcacggaaa aactataaga tgatagatgg caggctgctc
    52081 cagaccgata aaaagtattc tcatctgaaa cagaaagaaa agatttacac atggatgtat
    52141 gaagaagcga aactttatca tgacagaaat ggaaaatgcc cgaaacaaag acgaggaagc
    52201 tgctgctgta tcagccgtat atggtagaat tgaaaaggcc ggcatctgga ttctttatgg
    52261 tgaagtctcc agacattgga taactgctgg tctaatgcgc ctttgagtat ggggtgattg
    52321 attttaatac cggggaggta cggctttttg aggaaaatat tctgggattt acatttggcg
    52381 attattacat ttcctgtaac tgcaaggaca cggatgatga agatatgagg aatatagact
    52441 tgctgatttt ctactgtctt gaacggtatt aggactgaat ctgctgcgcg gagtgcggca
    52501 agagcgaccg gagggagtac acacaggccc tgcctgtgtg aaaagccata ccgcaaggtg
    52561 cattcgcaaa aaagaggccg cgcattttgc tgaggcttgg ggtgtcccca acaagcaaaa
    52621 cgagaacgcc gcaggcgggc aagtggtgga tcagaatatc cgtaaaggat attctgatcc
    52681 attgcttgcc agtattatcg ggatgttttg acaaccgctg taatcgaaac atcccaaaca
    52741 ttaagaaggt gttaattatt attatcttga tttaattcgt ttaaaccaat aatatagata
    52801 catacaccat gaaaagaagc gagtatccca atgacggatg attaaaatcc gaaaagaaat
    52861 cgctttgaaa ctacagggaa actacagact gttatgttat cttcttaaaa tggagggatt
    52921 tttatgtcga tacgaattct acttgtcgag gatgatgatc atatctgtaa tacagtaagg
    52981 gcgtttctgg ctgaggcagg atatcaggtg gatgcctgca cagatggaaa tgaggcatac
    53041 accaagtttt acgaaaacac ttatcaactg gttattcttg atattatgct gcccggtatg
    53101 aacgggcatg aacttttgcg tgaatttcgt gcgaaaaatg atactcccat tctgatgatg
    53161 acagccctgt cggatgacga aaaccaaatc cgggcgtttg atgcagaggc agacgactat
    53221 gtaacaaagc cattcaagat gcagatttta ctaaagcggg tggaagccct gctgcggcgc
    53281 agcggtgcgc tggcaaagga aatccgtgtc ggcaggctga cacttctgcc ggaggatttc
    53341 acggtacttt gtgacggtac agagctgccc ctgacacgaa aagaatttga aatccttttg
    53401 ctgctggtgc agaacaaagg cagaacctta acgcatgaaa tcattttgtc acgtatatgg
    53461 ggatatgact ttgagggtga tggcagcaca gtccacactc atatcaaaaa tttgcgggcc
    53521 aagctgccgg aaaatatcat caaaaccatt cgcggtgtag gataccgatt ggaggaatca
    53581 taataatgga aagaaaaggg attttcatta aggttttttc ctatacgatc attgtcctgc
    53641 tactggttgt cggtgtaaca gcaacactgt ttgcacagca atttgtgtct tatttcagag
    53701 tgatggaatt acagcaaaca gtaaaatcct atcagccatt ggtggaactg attcagaata
    53761 gcgataggct tgatattcaa gaggtggcag ggctgtttca ctacaataac caatcctttg
    53821 agttttatat tgaagataaa gagggaagcg tactctatgc cacaccaaat gccaatacat
    53881 caaatagttt tagacccgac tttctttatg tggtacatag agatgataat atttcgattg
    53941 ttgctcaaaa caaggcaggt gtgggattgc tttatcaagg gctgacaatt cggggaattg
    54001 ttatgattgc gataatggtt gtattcagcc ttttatgcgc gtatatcttt gcgcggcaaa
    54061 tgacaacgcc gatcaaagcc ttagcggaca gtgcgaataa aatggcaaac ctgaaagatg
    54121 taccgccgcc gctggagcga aaggatgagc ttggcgcact ggctcatgat atgcattcca
    54181 tgtatgtcag gctgaaagaa accatcgcaa ggctggagga tgaaatcgca agggaacatg
    54241 agttggagga aacacagcga tatttctttg cggcagcttc tcatgagcta aaaacgccca
    54301 tcgcggctac aagcgttctg ttggagggaa tgcttggaaa tatcggtgac tacaaagatc
    54361 attctaagta tctgcgcgaa tgcatcaaaa tgatggatag gcagggcaaa atcatttccg
    54421 aaatactgga gcttgtcagc ctgaatgatg ggagaatcgt acccatagct gaaccgttgg
    54481 acatagggcg cacggttgcc gagttgctgc ccgattttca aaccttggca gaggcaaaca
    54541 accagcggtt cgtcacagat attccagccg ggcaaattgt cctgtccgat ccgaggctgc
    54601 tccaaaaggc actatccaat gtcatattga atgcagttca gaacacgccg cagggaggcg
    54661 aggtacggat atggagtgag cctggtgctg aaaaatgccg cctttttgtt ttgaacatgg
    54721 gcgttcacat tgatgatact gcgcttccaa ggctgttcac cccattctat cgcattgatc
    54781 aggcgcgaag cagaaaaagt gggcgaagcg gtttaggact tgccatcgta caaaaaacgc
    54841 tggatgccat gagcctccag tatgcgctgg aaaacacctc ggatggcgtt ttgttctggc
    54901 tggatttacc gctcacatca acattgtaaa tatttaaaac ttaaatgatt ttgaccgaca
    54961 ggtataaccc tgccggtctt tttttgctgc tacaggaaaa ctacagactg gctacaggga
    55021 aagtacagat gcgcttgcca taataacaat cgtaccagcc acaaatcgta gttttattgc
    55081 aaaggaggca ttcgatcaaa tggaaaaaag caactatcat tccaatgcgg atcatcacaa
    55141 acggcatatg aaacaatccg tggaaaagcg ggcttttcta tgtgcgttca ttatttcgtt
    55201 cacagtctgc acgctgtttt tggggtggag actggcttcc gtattggagg caacacagat
    55261 accgcccatc cctgcaactc atacaggcag cagcactgac gtagtggaga atttggagga
    55321 aaacgctctt gccaccgcca aagaacaggg agatgaacag gaatggagcc tgattttagt
    55381 gaacaggcag aaccccatcc ccgcacagta cgatgtggag cttgagcaac tatcaaatgg
    55441 tgagcggata gatattcgga tttctcccta tcttcaagat ttgtttgatg ccgcaagaac
    55501 tgatggagtt tacccgattg tcgcatccgg ataccgaaca acagaaaaac agcaagaaat
    55561 tatggatgaa aaaattgccg aatataaggc gaaaggctac acctctgcac aggctaaagc
    55621 ggaagcagaa acttgggtgg ccgtgccggg aacgagcgag catcagcttg gtcttgctgt
    55681 ggatatcaat gcggacggaa ttcattcaac aggcaacgag gtttatagat ggctggatga
    55741 aaacagctat cgttttggtt ttattcgtcg ctacccacca gacaagacag agataaccgg
    55801 tgtgagcaac gagccgtggc attaccgata tgttggcatc gaagctgcca cagagatgta
    55861 caaccaaggg gtttgccttg aggaatattt aaaaccagaa aaatgagaaa aggatgttat
    55921 gttatggaca gaaaaagatt gacacagcgt ttcccgttcc tgcttccaat gagacgggcg
    55981 cagagaaaaa tgtgctttta tgcgggaatg agatttgacg gctgtcgcta tgcgcagacg
    56041 ataggggaaa aatcgctttc ccatttgctc tttgaaacgg attgcgcatt atacaaccac
    56101 aataccggat ttgacatgat ataccaggaa aacaaggtgt tcaacttaaa gctggcagca
    56161 aagaccttaa acggcttatt gataagaccg ggggaaacct tttctttctg gtggctggta
    56221 cgccatgcgg acaaagatac cccctataaa gacggcctta cggtgaccaa tggtaaactc
    56281 accaccatgt cgggcggcgg tatgtgccag atgagcaatt tactattttg gatgttcctg
    56341 catacgccat tgacaattat ccagcgcaga ggtcacgaag taaaggagtt tccagagcca
    56401 aacagtgacg agattaaagg ggtggatgca accattccag agggctggat tgatttaaaa
    56461 gtgcgaaacg ataccgactg cacctaccaa atatgggtga ccttagatga tgagaaaatc
    56521 atcggtcagg tgtccgccga caaagagccc caagcattat acaaaattac aaatggcagt
    56581 atccagtatg tccgtgaaag tggcgggatt tatgaatatg cccaggttaa acggatgcaa
    56641 gttgccttag gtaccgggga aataatagat tgcaagctgc tttatacaaa caaatgcaaa
    56701 atctgttatc ccctcccgga aagtgtggat attcaggagg agaaccaatg agaaaaagtg
    56761 tgggcattac tgtttttgga tgcgaacagg atgaggcaaa tgctttccgc gctttatcgc
    56821 cggattttca tattatccct acgctgatta gcgatgcgat atcggcagac aacgcaaaat
    56881 tggccgctgg caatcaatgc gttagcgtag gccataagtc cgaggtttcc gaggcgacaa
    56941 ttcttgcgct gagaaaggtc ggggtaaaat acatttctac ccgcagcatc ggctgcgatc
    57001 acattgatac gactgccgcc gagagaatgg gaatctcggt tggcacggtt gcgtattcgc
    57061 cggacagcgt tgcggattat gctttgatgc tgatgctgat ggccatacgg ggtgcaaaac
    57121 ccaccatgca cgccgtggcg caacaagatt tcagattgga tcgtatccgg gggaaagaac
    57181 tgggggatat gactgtggga gttattggaa ccggccatat cgggcaagcg gtcgtcaaaa
    57241 ggctgcgggg atttggatgc catgtgctgg cctatgataa cagccgaaaa atggatgcag
    57301 attatgtcca gcttgatgag cttctaaaaa acagcgatat tgttacgctc catgtgccgc
    57361 tttgtgcgga tacccgccat ctgatcggtc agaagcaaat tggagagatg aagcaaggcg
    57421 catttttaat caacaccgcg cgcggagcgc ttgtcgatac cggggcgctg gtggaggcgt
    57481 tggaaagcgg aaagctgggt ggtgcggcac tggatgtgtt ggagggcgag gatcagtttg
    57541 tttataccga ttgctcgcag aaagtgcttg accatccctt tttgtcgcag cttttaagga
    57601 tgccgaatgt gatcatcaca ccccatacgg cgtactacac tgagcgtgtg ctgcaggata
    57661 ctacagaaaa aacaatcagg aattgtctca attttgaaag gagtttacag catgaataga
    57721 ataaaagtcg caatcatctt cggcggttgc tcggaggaac atgatgtgtc ggtaaaatcc
    57781 gcaatagaaa ttgctgcgaa cattgatacg gaaaaattcg atccgcacta catcggaatt
    57841 acaaaaaacg gtgtatggaa gctatgcaag aagccatgta cggaatggga agccgacagt
    57901 ctccccgcca tactctcccc ggataggaaa acgcatgggc tgcttgtcat gaaagaaagc
    57961 gaatacgaaa cacggcgtat tgatgtggct ttcccggttt tgcatggcaa attcggggag
    58021 gatggtgcga tacaggggct gtttgtattg tctggtatcc cctatgtggg ctgtgatatt
    58081 caaagctccg cagcttgcat ggacaaatca ctggcctaca ttcttacaaa aaatgcgggc
    58141 atcgccgttc ccgaatttca aattattgat aaaggtgaca agccggaggc gggtgcgctt
    58201 acctaccctg tctttgtgaa gccggcacgg tcaggttcgt cctttggcgt aaccaaagta
    58261 aacggtacgg aagaacttaa cgctgcgata gaagcggcag gacaatatga tggaaaaatc
    58321 ttaattgagc aagcgatttc gggctgtgag gtcgggtgtg cggtcatggg gaacgaggat
    58381 gatttgattg tcggcgaagt ggatcaaatc cggctgagcc acggtatctt ccgcatccat
    58441 caggaaaacg agccggaaaa aggctcagaa aatgcgatga ttacagttcc cgcagacatt
    58501 ccggtcgagg aacgaaatcg ggtgcaggaa acggcaaaga aagtatatcg ggtgcttgga
    58561 tgcagagggc ttgcccgtgt tgatcttttt ttgcaggagg atggcggcat cgttctaaat
    58621 gaggtcaata ccatgcccgg ttttacatcg tacagccgct acccacgtat ggtggccgcc
    58681 gcaggaatca cgcttcctgc actgattgac agcctgatta cattggcgtt aaagaggtga
    58741 taagtatgga aaatggtttt ttgtttttag atgaaatgtt gcatggcgtc cgttgggatg
    58801 ccaagtacgc cacatgggat aacttcacag gaaaaccggt ggatgggtat gaggtgaatc
    58861 gcatcatcgg cacaaaggcc gtggcgtttg ctctgcgcga ggcacaaatc catgcggctg
    58921 cgcttggcta tggcttgctt ttatgggatg gatatcggcc aagaactgcg gtggactgct
    58981 tcctgcgttg ggcagcgcaa ccggaggaca atctcacaaa agaaaaattt taccccaata
    59041 tagagcgagc cgagttgatt acaaagggtt atgtggcctc acaatccagc catagccgtg
    59101 gaagcgcaat tgatcttacg ctctaccacc tggatacagg ggaacttgtt tcaatgggaa
    59161 gtaacttcga ttttatggac gaacggtcgc atcatacagc aaaagggata ggggatgcag
    59221 aggcacaaaa tcgaagatgc ttgcgtaaaa tcatggaaag cagcggattt cagtcttatc
    59281 gctttgaatg gtggcactat aagttgattg atgagccata ccccgatacc tattttaatt
    59341 ttgccgtttc ataatgaaag tattggtttt gtatcagatt ttggatttga taaaatctat
    59401 gctggtcatg gacaatcttt tactggaaag tggttaaagc atcttttgta atacattgca
    59461 aattatagta ttcaatctgc ccagcggcag aaaagaaaaa aaccgctgtt gggcagacct
    59521 ttcccacgcc cattttgtat tttgctggcg gcggttttga gaaatcaagg ccgccgttct
    59581 tttctggcag caaaaggtgt ggcggcccat aggaggatgc cgctatgctg ttttgctatt
    59641 gccatgctct aatccacttc caccactcaa aaaagccttg cccctc
//
LOCUS       DACDYI010000007        59393 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.007, whole genome shotgun sequence.
ACCESSION   DACDYI010000007 DACDYI010000000
VERSION     DACDYI010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.007"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(59..187)
                     /locus_tag="IWM52_000420"
     CDS             complement(59..187)
                     /locus_tag="IWM52_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP9972703.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="IWM52_000421"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="IWM52_000421"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP9972704.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="IWM52_000422"
     CDS             complement(1674..2729)
                     /locus_tag="IWM52_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP9972705.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="IWM52_000423"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="IWM52_000423"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAP9972706.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="IWM52_000424"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="IWM52_000424"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAP9972707.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="IWM52_000425"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="IWM52_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAP9972708.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="IWM52_000426"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="IWM52_000426"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAP9972709.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="IWM52_000427"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="IWM52_000427"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAP9972710.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="IWM52_000428"
     CDS             10491..12050
                     /locus_tag="IWM52_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAP9972711.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12161..14092)
                     /locus_tag="IWM52_000429"
     CDS             complement(12161..14092)
                     /locus_tag="IWM52_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP9972712.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="IWM52_000430"
     CDS             complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="IWM52_000430"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP9972713.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15021..15773)
                     /locus_tag="IWM52_000431"
     CDS             complement(15021..15773)
                     /locus_tag="IWM52_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9972714.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15932..16141)
                     /locus_tag="IWM52_000432"
     CDS             complement(15932..16141)
                     /locus_tag="IWM52_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972715.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16152..17480)
                     /locus_tag="IWM52_000433"
     CDS             complement(16152..17480)
                     /locus_tag="IWM52_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972716.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(17845..18432)
                     /locus_tag="IWM52_000434"
     CDS             complement(17845..18432)
                     /locus_tag="IWM52_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAP9972717.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="IWM52_000435"
     CDS             complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="IWM52_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAP9972718.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20012..21301)
                     /locus_tag="IWM52_000436"
     CDS             complement(20012..21301)
                     /locus_tag="IWM52_000436"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAP9972719.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21455..22387)
                     /locus_tag="IWM52_000437"
     CDS             complement(21455..22387)
                     /locus_tag="IWM52_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972720.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22544..23203)
                     /locus_tag="IWM52_000438"
     CDS             complement(22544..23203)
                     /locus_tag="IWM52_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP9972721.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="IWM52_000439"
     CDS             complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="IWM52_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP9972722.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24244..24534)
                     /locus_tag="IWM52_000440"
     CDS             complement(24244..24534)
                     /locus_tag="IWM52_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972723.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            24944..26749
                     /gene="glmS"
                     /locus_tag="IWM52_000441"
     CDS             24944..26749
                     /gene="glmS"
                     /locus_tag="IWM52_000441"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAP9972724.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(26821..28176)
                     /locus_tag="IWM52_000442"
     CDS             complement(26821..28176)
                     /locus_tag="IWM52_000442"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAP9972725.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(28200..29369)
                     /locus_tag="IWM52_000443"
     CDS             complement(28200..29369)
                     /locus_tag="IWM52_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972726.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(29366..30253)
                     /locus_tag="IWM52_000444"
     CDS             complement(29366..30253)
                     /locus_tag="IWM52_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAP9972727.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(30442..34122)
                     /gene="nifJ"
                     /locus_tag="IWM52_000445"
     CDS             complement(30442..34122)
                     /gene="nifJ"
                     /locus_tag="IWM52_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAP9972728.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            34320..34841
                     /locus_tag="IWM52_000446"
     CDS             34320..34841
                     /locus_tag="IWM52_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP9972729.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(35540..36105)
                     /locus_tag="IWM52_000447"
                     /pseudo
     CDS             complement(35540..36105)
                     /locus_tag="IWM52_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(36284..36940)
                     /locus_tag="IWM52_000448"
     CDS             complement(36284..36940)
                     /locus_tag="IWM52_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972730.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            complement(37508..38443)
                     /gene="whiA"
                     /locus_tag="IWM52_000449"
     CDS             complement(37508..38443)
                     /gene="whiA"
                     /locus_tag="IWM52_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAP9972731.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(38477..39475)
                     /locus_tag="IWM52_000450"
     CDS             complement(38477..39475)
                     /locus_tag="IWM52_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAP9972732.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(39472..40356)
                     /gene="rapZ"
                     /locus_tag="IWM52_000451"
     CDS             complement(39472..40356)
                     /gene="rapZ"
                     /locus_tag="IWM52_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAP9972733.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(40491..41222)
                     /locus_tag="IWM52_000452"
     CDS             complement(40491..41222)
                     /locus_tag="IWM52_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAP9972734.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(41226..42491)
                     /locus_tag="IWM52_000453"
     CDS             complement(41226..42491)
                     /locus_tag="IWM52_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAP9972735.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(42754..45570)
                     /gene="uvrA"
                     /locus_tag="IWM52_000454"
     CDS             complement(42754..45570)
                     /gene="uvrA"
                     /locus_tag="IWM52_000454"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAP9972736.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(45580..47574)
                     /gene="uvrB"
                     /locus_tag="IWM52_000455"
     CDS             complement(45580..47574)
                     /gene="uvrB"
                     /locus_tag="IWM52_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAP9972737.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            47833..49335
                     /locus_tag="IWM52_000456"
     CDS             47833..49335
                     /locus_tag="IWM52_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9972738.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            49337..50074
                     /locus_tag="IWM52_000457"
     CDS             49337..50074
                     /locus_tag="IWM52_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9972739.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(50442..51608)
                     /gene="dnaJ"
                     /locus_tag="IWM52_000458"
     CDS             complement(50442..51608)
                     /gene="dnaJ"
                     /locus_tag="IWM52_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP9972740.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(51759..53588)
                     /gene="dnaK"
                     /locus_tag="IWM52_000459"
     CDS             complement(51759..53588)
                     /gene="dnaK"
                     /locus_tag="IWM52_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAP9972741.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(53639..54202)
                     /gene="grpE"
                     /locus_tag="IWM52_000460"
     CDS             complement(53639..54202)
                     /gene="grpE"
                     /locus_tag="IWM52_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAP9972742.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(54296..55339)
                     /gene="hrcA"
                     /locus_tag="IWM52_000461"
     CDS             complement(54296..55339)
                     /gene="hrcA"
                     /locus_tag="IWM52_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAP9972743.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(55574..56740)
                     /locus_tag="IWM52_000462"
     CDS             complement(55574..56740)
                     /locus_tag="IWM52_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAP9972744.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            complement(56756..56983)
                     /locus_tag="IWM52_000463"
     CDS             complement(56756..56983)
                     /locus_tag="IWM52_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAP9972745.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            complement(57370..57626)
                     /locus_tag="IWM52_000464"
                     /pseudo
     CDS             complement(57370..57626)
                     /locus_tag="IWM52_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            complement(57969..58992)
                     /locus_tag="IWM52_000465"
                     /pseudo
     CDS             complement(57969..58992)
                     /locus_tag="IWM52_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
BASE COUNT        16402 a        12848 c        10314 g        19829 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcccgc ttttttgaag taagcgtcat aataaccagc acttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tctgagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 agttggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcttcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgcttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttctgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgacagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcaattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg tgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ttccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggga attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat atcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt tgccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccgatcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatatgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atggaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaaggtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtggtg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 aagaagagac tgggaaaaac cagcctcttc ttttttattt ctatgcttct gctgctgcag
    12181 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    12241 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgcca tgaggagcca
    12301 gtaatttgat cccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    12361 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    12421 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    12481 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    12541 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    12601 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    12661 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    12721 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    12781 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    12841 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    12901 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    12961 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    13021 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    13081 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    13141 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    13201 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    13261 atactggtgc agtaccacta agggctagat tgatcagctc ttctggttta cggataccat
    13321 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    13381 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    13441 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    13501 tcagagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    13561 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    13621 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    13681 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    13741 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    13801 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    13861 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    13921 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    13981 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    14041 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    14101 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    14161 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    14221 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccggcaa tcattgaatc
    14281 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    14341 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    14401 agctagcaat tttttaccat acggaatgat ttcttctaca gattggaagg ttgtattgta
    14461 aagctcagct aattcgtgat tgtttggttt gatcaacaat ggtttcttaa caagtgcatt
    14521 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    14581 aatcagttct tgataaaatc cttttcgcag gctagccggc gcgcttcctg acaatacgac
    14641 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    14701 aatagatggg cccaaaccgt taatttcagt ttcagtatct gattttagtt tgatattgat
    14761 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    14821 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    14881 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    14941 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    15001 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    15061 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    15121 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    15181 gaatttttca tggctaaacg tttaacagct gcctcttctg ggtccggagt cgtgaatcct
    15241 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    15301 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    15361 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    15421 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    15481 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    15541 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    15601 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    15661 cgtcttatcg ttgattcaga agcattaaaa aggtttacaa gatcttgtga tttcacgatt
    15721 ttctgctgtt caagcaagtc taggattttt tgtctacgtt cctctgtaag cattttcacg
    15781 atcacctcaa tacaaataat agcatatttt ttttctactc gcaatataaa tcagtcaaaa
    15841 acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcaa aaagactggt
    15901 tttttatttt tttgtctatc atcaagccgt tttatcgttt tttattttcg cttcttgttt
    15961 ttttcatata aacagaagca ggttttcttt ccttatgaag aagataataa taaagtccca
    16021 tcccgccaaa catgaccaaa aaaccgatga ctgatcgaat aggaaaatta ggaacaaaaa
    16081 agagacagac ggataataat gatgccggaa ccattattcg ataatagagt tgtttatcca
    16141 ttttcttcac ctcatatttt ttgtaaagta atcaattgtt ctaattctcg gtaatctaaa
    16201 aattgcacac gcagatgctg ctcacctttc ttgacatcga attggatact tcgccgcttt
    16261 ttctttattc gtttgaaggt aacatcagag agtctatgtt tgatttcctt taacggtaat
    16321 tctgacaggg attctgaagg aacatagatt cttaaggata aaagatactc ttgatatttt
    16381 tcatctgtta cctctacttc tagatattcc ctaatataat aacaataagc ttcgattcca
    16441 ttatccaaag caacagtcgg atcgataact ttatagcaca tcatgcgcga gcgattctcg
    16501 tcaacccaag gaaaatacgc ctgagcattt agtgttccgc gaacacgcct ttctttataa
    16561 tctccttgtt gaatatcata actttttgtt acaaaaaatg aagcattctt tttaggaaca
    16621 ggttgcattt gtgcattctg atactggaca gattggatcg tatggtcagt gtaaatgtat
    16681 tcaacgaatg gcggtttcaa taaaccgaca ccattgtccc aagcaagatg atcaaaccca
    16741 agctggtccc taaaatagga ttgaaacatt tgattgtttg gcttttgcca gttaaagggg
    16801 tctcgataaa gctggataca tatgacttcg ttttctaatt ccttcacttt ttctacttgt
    16861 tggatgtcga gaaaaatcgg tttagggatg atttgatgat aatagcgact ataatacatt
    16921 tcccaacacg aagtgaaaca taaaccttgg taaaaaagat cgtatcctgg aaattggttg
    16981 caatcgacta ctacttcccc gttttgttgt ttcattttag gtaaatgttc gatttcatcc
    17041 tttttctcaa acaatagccg ttgagagatc tgttttgtat tgtgatagat aaactcgcta
    17101 taagctcgca tataggcgaa caccccatga gcaatcattt ttttctgaat gaagtctcga
    17161 atcaaaaatt gcttttcttt gaaaacagag atagacaata cattttttat caataattga
    17221 ttatcttcca agcgaaaact acattcgttc tcgtcatttc ctacaactaa ccaactttcg
    17281 tctcttaccg atgctagcat tattttttct tgaagctttc gaagatcata cggatccgct
    17341 tgttgtgtga aagagcggat aaatgttggc tggatcaaca gacgtgtcaa aatagcccaa
    17401 tcgcttattt cctgtttttt ttcttcttca tttaagtctg tgagctggat agtcatcaat
    17461 aatttatctt cgccaatcat tttctcacct ctttaaaatc tttttataat ttaattataa
    17521 ccaacgataa agaaaaacta aaactttatg tcttagaaac caatttggac gattacatga
    17581 tagactaagt aaaaagaaac aaagaggtgt ttacaatggg gttgaaattt gaacgtacag
    17641 ataatttaga taaacttttc gaatcatttg ctgtcgatcc ggaaaaaaca gttgcaccaa
    17701 aagaagagat cgatcgattt ctaaaaccac agccacaaaa tgaaaagaaa aaagacaaga
    17761 aacaaaagta agttaagctc aaaaaaagac ttaccagcca aacttttatg aaagagtttg
    17821 actggtaagt cttttttatt ctttttatga ttcaattgct tgttcgattg ctgcccacgc
    17881 ttcttctttt ccctgttttg taacggaaga aaagagaata aacgtgtcat tgcgatcaaa
    17941 atccaacgct tttttgattt cgctctcatg cttgttccat ttaccacgag ggatcttatc
    18001 tgccttagtg gcaacaacaa ttacagggat atcataatac ttcaaaaatt gatacatctg
    18061 gatatcttct tgagaaggct tatgacgcaa atctactaaa gaaacgaccg cacgtagctg
    18121 gtctctagtc gttaaatatg tctcgatcat ctttccccat tttgctcgct cagttttgga
    18181 tacttttgca tacccatatc ccggaacatc gacaaaatgt aatgcgttct cgatcaaata
    18241 aaaattcaat gtctgggtct ttcctggctt accggaagta cgggctaaat tctttcgatc
    18301 aatcaatgtg ttgataaaag aagatttccc tacatttgat cgtcctgcca gagctatttc
    18361 tggcaattgt gtatctggat attgttttgg tgccactgca cttatgataa tttcagcttg
    18421 atggactttc atggtctctc tccttaacaa ggtgatgaat aaagcaagcg gctgtcgcaa
    18481 aagccaaaat gacttttacg gcagcctctt ttcatcaacc cgcttttttc tctgaatctt
    18541 tatagattac tgttggctca ccagacaatt cagctgcttc ttttgtgata atcacttttt
    18601 cgatgttttc atttgaaggg atatcaaaca tcacatccat catgatttct tcaatgatgg
    18661 aacgaagtcc tcgagcaccg gtattgcgtt cgattgcttt tttcgcaatg gctctcaacg
    18721 cttcaggttc gaattcaagt tcggtattat ctagagaaag aagtttttga tattgtttga
    18781 ctagcgcatt tttcggctca gtcaagatcc gtaccagatc atctgttgtc aatttctcta
    18841 aagctgccat aacaggtaat cgtccgataa actcagggat caatccgaat tttagtaaat
    18901 cttcaggaat gatatgctgc atgacacttt cgtcttcaga aagtttttgg ttttgtgtac
    18961 caaatccaat cgtcttttcg cccatacgat tcttcacaat cgtttcgatg ccatcaaatg
    19021 caccacctac gatgaacaat atgttcgtcg tatcaatctg aataaattct tgatgtggat
    19081 gtttacgtcc tccctgaggt ggcacgcttg caacagttcc ttctagaatt ttcagcaagg
    19141 cttgttggac accttcgccg gaaacgtcac gagtaataga tacattttcg cttttacgcg
    19201 caattttatc gatctcatca atatagatga tccctttttc tgcacgttcc acattgtaat
    19261 cagcagattg aagcaattta agcaaaatgt tttctacatc ttcccccaca taaccagctt
    19321 ctgtcaaact tgtcgcatct gcgattgcaa aaggtacatt caatgttttt gctaatgttt
    19381 gtgctaaaaa tgttttacca gaccctgtag gtccgatcaa gcagatattg cttttttgca
    19441 gttcaacatc atctgtgttt tcttgtgctt gctgattgac acgtttatag tgattgtata
    19501 cggcaacagc taacgagcgt tttgcgcgat cttgtccaat cacgtagtta ttcaatactt
    19561 caagaatttc tagtggttta gggacttcag tgaattcgcg tacagcttct tcataaaatt
    19621 cttcatcaat gatttcttta cataggtcga tacattcatt acagatgtag acccctggac
    19681 cagctacgat ctttttcact tcttcttggg ttttcccgca aaaagaacag cgtacggtct
    19741 cattgctact tggattgtca tacatggtct tcacccctta acttgcaaaa agtatgcaag
    19801 aaactatcaa agactttaca gtcttttact atcataacat agtttagtaa aaagaaaaag
    19861 tgtttaacaa aggatcaact gacctatacc tttgcatttt ttactcttaa caaaaaataa
    19921 ggaactgtga caaaatcgtg tcacagtccc ttatttcgaa taaacaattt tctagaagta
    19981 gcttcttcag aaatgattta ttacttgatt tctattcttt cgcttctgtc gctgtacctg
    20041 tgatcaattc gattgctttt ttcattgtca catcatgttc cagcatatct tctgtcaaca
    20101 cgcgtttgat ttgttcgatt ggcatgttgt atgtttctga taattcttgg atttctgcat
    20161 tgatttcttc ttctgttgct tgaagatttt cagcagctgc aattgcttcg atcacaagat
    20221 ttgttcttgt gcgcatttct gcttcacctt cgaattgttt atgcaagtct tcttctgtag
    20281 aaccagttaa ttgatagtac atttcaggag agataccttg acgttgcatg ttgttcaaga
    20341 attcgtccat tgaacgatgt acttcatcat gaaccatcac gtgtggcaat tcaacgattt
    20401 ctgcgttttc aacagccata cggatcgctg cttcatcttt tgcatcgtcc gctgcttttt
    20461 cttttgtttc tactaattct ttgcgatatt tttctttcaa ttcgtctaat gttgatactt
    20521 cgtcatcaac atcttttgcg aattcgtcat ctaattcagg cacttctttt gcttttactt
    20581 catggattgt cactttgaag actgcttctt ttccagcaag atcttctgct tgataatctt
    20641 ctgggaaagt tacatttaca tctagacttt cacctgattt atgaccaact aattgatctt
    20701 caaaacctgg aatgaatgag cctgaaccta attcaagtga gtaattttcg ccttttccgc
    20761 catcaaacgc ttcgccatct acaaagcctt cgaagtcgat tactactgta tcgccatttt
    20821 cagctgcagc atcttctttg ataactaatt cagcttgtgc ttcttgttca cgtttgatac
    20881 gtgcatccac gtcagcatct gttacttcac ggtcttgttt ttcaacaact agatctttat
    20941 attgtcctaa ttttacttct ggtttcactg ttacttcagc agtgattacc cagtcttgac
    21001 ctttttccat gctttcaaca tcgatttttg gttgagaaac aggatcgatg ccagcttctt
    21061 tgacagcagc ttcataagct tctggtaata cagcattcaa tgcatcttca tataaagctt
    21121 cttcaccgta catacggttg aatacttgac gagaaacttt ccctttacgg aatccaggta
    21181 cgttcaagct ttttttcact ttattaaacg cagttgttaa tcctttttgg atttcgtctt
    21241 gcgcaatcgt aaattttaaa acaccatcat tcgtgccttt tttttcaaat gttgcagtca
    21301 tttaattccc tccgagttta gaaattcatt ttatacatca aaaacgtttc atgcatacct
    21361 ttaaatagta cactaaccct ttcaaattgt aaacatattg aatggataga atccttgttt
    21421 ttcataacat ttttatcttt ttcactgata aaatctaatg gaagaagatc atttgctgca
    21481 aaattttttc tctttttttt aaaaaatctt gtttttcatc ttctgtacct tcatacatcg
    21541 tttttcctgt acacgcctct acagtcagtt gaaaccagcg cttcgggtct tcgatgtatg
    21601 tatcatagac aggataaaca attgcagatt gcaacgtgaa ttcttcattt aacacggaac
    21661 taagcagcgc atcttcatta ccgtaacgat ctgaaaatac acataattgc tggtaacttg
    21721 cctgctgctg cggctttggc agatcttttg gtatgacttt gactaacttt tcttcaatcg
    21781 tcaaatagaa aacaggatgg cattcgttca attgcctaag tgtctccagt aattttgccc
    21841 ttgctaaaat atggacctct ggcgatacaa taagtttact cgctgtttgg ataaactcat
    21901 gataaggaag ctgttcgatc tcatcaatag acgcaagctg acgtgcaggt tccaacgaag
    21961 gaagttcctc cgatttattg tggagtgcct tgatcttctg cctctccatt tgtccggata
    22021 attcttctgc aaattggatt ttttcaatga cccgctgctc gaaagcttca taaaaatctg
    22081 tacttgctat caattttcta gcatataaaa aatcttgtgt actgattaac aagtcaaaat
    22141 aaaattcagc aaactcctca ttttgaagat actgtttttc atgcaagagt gcttgcttca
    22201 atgcttcttt tttctcgtcc aactcgtaaa gacaaaaaac aagcaggcta ttagcctcaa
    22261 agctttcttt caactcgtaa gctcgttgaa ataattcttt tgcctgctga tattgctggt
    22321 ttgctagtgc ttgttgtgct actcgcatta aattctcata tttttctgga aaatcaatcg
    22381 gcttcacgca gtgtcctcct cttgctcatg ttcagctcat gttcaatagt agcaaaacaa
    22441 aaaaagcatg acaattaaaa ataactgaag caccctctaa aagctagatt ttagatctga
    22501 cttttagaag gtgctttcaa ctgttactgc tttttattgc tcatcagctg ttgacttcct
    22561 ggttgaatgc tttgatcaga ttgatcgtaa caacacttgt attagtgaaa aaatcgccta
    22621 gatgttgctt tttctgatta aagattgctg gaagataccc taacatcagc aaaggaaatt
    22681 ttagaatgaa ccggcaagca ccttctctaa ctaaaacggt tgaccagcta agttcttctt
    22741 catcaaagct gatgactcga ataccaaaga tcattttccc aatcgtttga ccatgattga
    22801 atttcgtcaa taaaataaaa tacgctagat aaataagtaa ggctgtgaat ccgtagatac
    22861 tcagataaga atctgaaaaa ttaagccaac ctagattttt ggcaatacca agtgttaaac
    22921 gcgtaatagc tccgatacac aaaagatcga ccaagaaagc aaacattcgg ataaaaaaac
    22981 ctgcatagaa atacttagga aaatcatttg cttttggcgg ttcatttctt gtgtccgtat
    23041 actttttcca tttacgttgt ttctttttga tttcttcggg cgtcaaaggc tctctttcaa
    23101 aagagctcag taccttttgg gaaaagttgg agtcaggtcc tacagcaggc tgctgttgct
    23161 tatctttttc ttgttcctta ttttcatttg aaagctctga catgcttact cacctccgta
    23221 ataatacatt gcttttggtg attccgctgt ccctagattc tcaagaacgg ataagatctg
    23281 actagtctca gaaggcttca atccctggaa ttctgctagt ttacttccta accatgtact
    23341 agcaaaccca gatggcgtgg aagaatattc tactagttcc gcatcttcaa gctgttgttc
    23401 ctttcgcata gcttttaaag catcttcagg aaatcccaat tcatcgacta aacctacttc
    23461 ttttgcttgg acaccatcat aaatccttcc atcagcaatt tttttgactt cttcttcaga
    23521 tttattccgt ccctcgctga cgattgctac aaaacgacta tatgcgctgt cgatatatgc
    23581 ttgaagcacc gcttcttctt cttttgtctc tggtcgcgtt cctgaaccca tatctttcaa
    23641 cgctccgctt ttaactgttg tatcttccac acccagtttt tctagaagtc ctgaataatt
    23701 caatcctgac atgatcacac caattgagcc ggtcactgtt tcttctgttg caaagatttt
    23761 atctgcttga gcagaaatat agtagccacc gctagctgcc atgtttttca tgctgacata
    23821 cattgggata tcatgttctt tacgaatcgt atccaatttc ttagcgattt ccgcactttc
    23881 gtagacaccg ccaccaggag aatttacttc taaaaagata cctttgactg ttggatcttc
    23941 ttcaatcgcc ttcagttgtt ccataaaatt tttatgattg taaccttcag aagcaaataa
    24001 acctgaagac cctcccgcag caatcgttcc atctactgat aattttacga ttttttgcgt
    24061 actgtctccc tcttccaaca catttgaagc tagttcttca tccccatata accaactgtt
    24121 caatccctca atttgatcgt tttcctgagg cttagtcaaa ctagctgaaa ctaacgaaac
    24181 cacaaataat cctgcagcaa tcagaactgc tgcccaacgt ctcttattca ttctttctcc
    24241 tttctaatac aagtttttta tgacgatcac agtttctgac tgctcagaag tgtcgaaccg
    24301 ctctgctttt tcttcccgcg catacccgga aaacgactct tttgtcaatt catcttctac
    24361 cgtaaactca cgagcttcag caacttccaa ggcatcaggt gcgatttctt ggatgctatc
    24421 ccagccagta tctaaaaata ttttcgtatc atctgataca ccagctgatg cttcgaattt
    24481 ggacaattgt ttttttaaca ttcccagtgt catgatctca atttttgaat ccatttttac
    24541 tcctcctcta tccgattact ttctattgta ccgaactcct gaaaaagatg atagataaaa
    24601 acccattatt ttttgacata aaaagagcat ttgacaaagc atttgatata taccaattta
    24661 ttttttattc tttttttgtt gacaagaaca ttcgcaaaag gttatgattt taaatgtttc
    24721 cagataaaaa acaaaagagg aggatagtta taccttttag gatagcgcca ggtctgtgta
    24781 aacagatgac gaggagaggg tttatcgaaa gattcggcgg atggccctag ggactgcact
    24841 ctacagaaaa tccacaaaaa atatctgcaa agataaaaca aaaggatttt cctgcagctg
    24901 gaagcattca tacgaattca ttatagaaag taggatgttt tttatgtgcg gaattgttgg
    24961 aatgattgga ttaaaaaatg taacaccagg attgattgac ggtctagaaa aattagaata
    25021 ccgtggttat gattcagctg gtatctttgt ctcagatggc acaactgatt atctggtcaa
    25081 agcacaaggc agaattcaaa atttgaaaaa caaaataaat attgatacta caggaaatat
    25141 aggtattggt catacacgct gggcaactca cgggcaacct tctgaagaaa acgcccatcc
    25201 acatacttcc caaagtggac gcttcgtgct cgttcacaat ggcgtaatcg aaaactttga
    25261 agaacttaaa attgcttatt tagcaaatga ccactttatt ggtgagaccg atactgagat
    25321 tatcgctcat ctaattgaaa ctttcgcgaa ggatactaca acgcaagagg cgtttttaaa
    25381 agctttgcgg gtcatcaaag gttcttacgc tttcgctttg attgatcgga cagcacctga
    25441 tgttatttac gtcgctaaaa ataaaagtcc actattaatc ggactcggcg atggattcaa
    25501 tgtgattgcc agcgatgcaa tggctatgct tgctcatacg aaggaattcg ttgagattga
    25561 agatgaagag atggttaccg tcacatcaga aaaagttatt attcaaaatt ttgcaggtga
    25621 catcgtagaa cgttcttcat tcgaagcaca agtagattct tcagatatcg agaaaggtac
    25681 ctatcctttt tatatgctga aagaaatcga cgaacagcct attatcatgc gtcgtatcgc
    25741 tcaaaaatac gtaacagaag acaatcgtgt ctctcttgat caaaagctgg tcgatacatt
    25801 gtctgatagc gatcgaatct atatcgttgc ttgcggaact agttaccatg cggggttagc
    25861 aggtaaacag actctggaaa aattgacaca gatccctgtt gaggttcatt tagccagtga
    25921 atttggctat aacacacctc ttctttcaaa aaaaccattc tttattttct tgagtcaaag
    25981 tggcgaaaca gcagatagcc gccaagttct ggtaaaaatc aaccgtctcg gctacccttc
    26041 acttacgatt acaaatgttg ctggttctac tctttcgcgg gaagcatcct ttacactgct
    26101 attgcatgct ggacccgaaa tcgcagtagc atcaacgaaa gcttatactg cacaaatagc
    26161 tgtactaact ttattagcca aagctatcgg tgataaaaaa gaatttgaaa cttctcttgt
    26221 attcgatatc actcatgaat tgagtttagt tgctaatgcg atggaatcgg tcatcgccca
    26281 aaaagattat ttagaagagt tggcagaagt atatctcagc gatacaagaa atgcctttta
    26341 cattggacgt ggcgctgact acaacgtttc tcttgaagct gctttgaaat tgaaagaaat
    26401 cagttatatc caggcagaag gttttgccgc tggcgaattg aaacatggaa cgattgcttt
    26461 gatcgaagaa ggaacaccag tgattggaat catcagtgaa gaagtgactg gtgcgcatac
    26521 tcgaggaaac ctgaaagaag tcgaaagccg tggcgctaaa acactagtta ttgtctcaga
    26581 gggattagaa aaggaaagtg accagctggt gctaccaact gtccaccctt atctaactac
    26641 tctaaccaca gtcgtaccga ctcaattact tgcatattac gcaacgctat tacggggatt
    26701 cgacgttgac aaaccacgta atttagcgaa atcagtgaca gtagaataat aaaagaacaa
    26761 ggaccagtgt cagacatctg acactggtcc ttgttctttt attttttatg aaaatatctc
    26821 ttaatccagt ccgatttctt ctttgactac tgccgcgatt ttatcgacat agtaattaac
    26881 tttctcatca gtaggcgctt ctgccatcac tcgaagtaat ggttctgtac cggaaggacg
    26941 aactaggatt cttccttctg atcccatttc agcttctgcc tcttcaatca cttttttgat
    27001 tgccggaaca tccatcgcac cgtttttatc acttactcgg atattcacta atttttgcgg
    27061 atagattgtt acctctgctg caagctctga taatttctta cctgtctgtt tcatgatatt
    27121 cagtaactga attcctgaaa gcatcccatc tccagttgtg ttaaaatcta agaaaatcat
    27181 gtgcccagac tgctcgccgc caaagttata atcgtttttg cgcatttctt ctacaacgta
    27241 acgatcacca acttgtgtaa cgacatcttt caaaccgatt ccttccaccg ctttgtggaa
    27301 tcctaagtta ctcatgacag tcgtaacgat cgtatctttt ttcaatcgat ttttttctgc
    27361 caagtattta gcacagataa acatgatttt atcaccgtcg acaatgtttc caagttcatc
    27421 caccgcaata atacgatcgc catcgccatc gaaagctagt ccagcatctg ctcctttttc
    27481 aacgaccatc tcagctaaac gttctggatg ggtagagcct acgccatcat tgatatttaa
    27541 cccgtctgga tttgtgccca tggtataaaa atctgtttca agatcggcaa acaaacgatt
    27601 aacacttgtt gaagttgctc cgtttgctgc atcgacacaa atggtcaaac cggaaaggtc
    27661 accgttgatc gtttgtacaa ggaattgaga atatttcaat aagccttctg gaaattcttc
    27721 tacagttccc aatccttcag ctgatggacg aggcaattca tccacttcag catctaataa
    27781 tgcttcaatt tctagctctt ggtcatctac tagtttaaaa ccatctgcac cgaaaaattt
    27841 gattccatta tcttgtgctg gattatgaga agcagaaatc atgacccctg cgcttgcttt
    27901 ttgcaaacgt gtcagataag caacacctgg tgtagagatc acacctagct ggaacacttc
    27961 gatacccacc gataaaagtc ctgaaatcag cgcttcttct aacatttgac ctgagattct
    28021 tgtatcccgc cctactaaca cgcgtgggcg ttttccttct tcttcatgct ggcttaggac
    28081 ataacctccg caccgtccca gcttaaatgc aagttctggc gtaagttcct tgttggcttc
    28141 accgcgtacg ccgtctgttc caaaatattt acccatagtt gtaaaattac tcctttaact
    28201 taattttctg aggaatttgt tctgctcatt tgtgttgatt cgcttgtttc tgatgtttct
    28261 tctgtacttg tggtcgagct gcttgtccca gattccgaac tggaagaaat ggttgttgtc
    28321 gatggttccg ttcctgaatc agcagatgaa atacttctac tcgtacttgt attttcttgt
    28381 gtccgactgc tcgtcgaagc agagctactg cttgacgttt gcaacacggg tgtaatctcc
    28441 acactaactg attcgggctt gatacttaac ccctcttcta ctggtatttc aattttacga
    28501 gttgttcgat gattgatacc tgagacattg atcggtacag caatactgtc accgatcgat
    28561 gcaagcaaat cgctagagcc gcttacttct gcttccagtt ttgaaaaact gaaatcatag
    28621 ctttctaccc cggcagctgg tgttccttgc tgtatcccat aaagtcgtac cgacttgctt
    28681 ggcttgatcg aatcagctct cactgttact tgcactggat cagagacgat acttaatact
    28741 tcccctgctg cattcagcgc ctgtattggg acctcactat taatcccatc atctgtgacc
    28801 atcgaagcgt ccacatcagc tactactcgt ttgatttctg ccaatgtttt gtcacctgta
    28861 gtgatagaga cttttttagg actaacggaa gtatcatcaa gttcatatcc agatggtgtg
    28921 aacgttgaag gaatcaacgt ttccacatcg aaatctttta ttaccttttt ctcaaccgtt
    28981 actgtaatag tcgatggttc gatctttgct gtgacagcac tgctcaaatt ttgaattttt
    29041 agcggaacct catgtgttcc ttcgcccagc ttagtgatat ctgctgtgac gcggaacatc
    29101 cgtgtatctt cgtttgcctc tgcattcaac tgaactcggt ttgcactgga aagtttgaca
    29161 gtcactgcat tttcatatcc atggatatag tagcgctttg aatcataaga aatcttgatt
    29221 ggcaccccag taactgtttg attatacgct tcatttccag aaagcgtatt tggaacggac
    29281 tggccatttg cattgaagta cagcgcaata ctgaacagca atgcaataaa actgtaaaga
    29341 atgcttgtcc gtttctcttt tgagatcatt tgcgatcccc tcccttagtc ataccgtcta
    29401 ggaaagattg aatcacgttt ttcttgtttt ttgttttctc ttcagcaatc agttctctac
    29461 gcaaaattgc caaatattct tgctgggata agccagccat cagttcatta tctaatgtaa
    29521 tgctgacatc cccagtttct tcagagacaa cgatggtaat cgcgtcgctg acttcgctta
    29581 taccgacagc tgcccggtgt cgagtcccga attcttttgg aatcaacata ctttctgata
    29641 gaggaagata ggcgcttgct actgcgatct ttccttcttt tacaataact gcaccgtcat
    29701 gcagcggtgt attaggaata aagatattga tcagcagctc tccagtgata tctgcatcta
    29761 atgcaattcc tgtctcaata tactcatcta atccagtatg ccgttcgata gtaattagcg
    29821 caccgatttt tcgcttggac atatattgga tggctttgtc aaaagacaag atcatcttct
    29881 catcttcctg ctgttcgctt tttgattgtt tgaaaagcga actgcgtcca agatgttcca
    29941 gtcctctccg gatttccggc tgaaaaatca cgatagcagc tatcaccccg tacgtaatga
    30001 cttggttcat cagccaagac aaggtgtgca gaccaatgat ttccgctacg attcggataa
    30061 caataaaaat agctacacct ttcagaagct gaatcgcttt tgtgcctcgc agcagttgga
    30121 tcagtttgta tacaagatac cagacaacca gaatatcaag tatgttcacc aaaaaattca
    30181 gagagaacaa gtcaccggaa aacatttgct gccaatagtc caaattaaaa agttccccga
    30241 tttgaaatga cataaaaagc ctctcttctc ttttgagatt tcctctctag tatatcatat
    30301 gttttaattt tagaggctga tctgctcgta tttttcttga gaaaaacgca taattttcag
    30361 gaaaattgca agtatgcgtt ttatttttct ctgcaataaa tatttttaaa cttcagagaa
    30421 ttattcatta tattttttct gttatttttc ctttttttct tttgtagctt gttccatttt
    30481 tttacgtcgt tctgctctgg cagctcttct ttcttctgct tcaaggtccg tcgttttttc
    30541 ttttctagtc ttaacgggta catcgataga ttcctctggt tcaagtcgtt tcttgatttt
    30601 ttctaattct cccgtcagtg cagcatagtt gtaaaaacgt gttttagcgt cttggatagt
    30661 cttttcaaat aaagcttttg caagatttgg atttgcgttc tctagcgagg aaaagcgaac
    30721 ttgtttcctc atgaaatcag gcatagcaga gaaatcaggt tttttgtagt ccaaagtcat
    30781 tgggttcttg tttttttcga ccattgcagg gttatatcga tataatgacc aatagccaga
    30841 agaaacagct tcttttgctt ctttaagcga ttctttcatt ccaccaagca aaccatgtgt
    30901 gatacaaggc gtataggcaa tgattaaaga aggtccagga tacttttctg cttcttcaaa
    30961 agcctttatc gtctgcattt gattggcacc tgaagcaatt tgcgccacat aaacatttcc
    31021 atatgtcatc gccatcattc ctagatcttt ctttgaagca tactttccac tagccgaaaa
    31081 tttagcgatt gccgaagctg gtgttgcttt tgaggtttga ccgccagtat tggaatagac
    31141 ttcgttatcc aaaacaagaa tattcacgtc tgctccgctg gcaagtacat gatcgatccc
    31201 gccaaaacca atgtcgtaag cccatccgtc tccaccaatc atccattggc tgttttttac
    31261 gaatagatcc tttctcttgt atatatcctc cagttcaggg acaattgatc gttcgctttc
    31321 gatcgcagct tgcagttttg ctgctctttg ctgcgttcct tcgctctcaa acatgtgtac
    31381 caaccagtct tccatcaatt ctcgtagttc ttctgatact tttggcaata cttctttgat
    31441 tgaacttgcc aaagcctcgc gacgagtctg tgccgctaga agcataccat aaccaaattc
    31501 agcattatct tccaataaag aatttgacca tgcaggccct tgaccacatt cattggtcgt
    31561 ataaggagaa gcaggagaag caccgcccca aatagaagaa cagcctgtcg cattcgcgat
    31621 catcattcga tcaccgaaca tttgtgtcaa taattttaca taaggtgttt cgccacagcc
    31681 tgagcaagcg cccgagaatt caagaagcgg ttgattgaat tgtgtagaca agacagtatt
    31741 ctttcctttg actggatttg ctttttgtct taatgtcatt gcaaaagccc aattcacagc
    31801 ttgttctttc tgttcttcat aaggcttcat cactaaagct ttttcttttg cgggacaagc
    31861 ctctacacac agaccacaac ctgtacagtc ttccactgaa acttggatac ggtattttac
    31921 gccgtctgcg cctcgcattt ctctcacgat aaatccttct ggcgcttctt ccatttcctc
    31981 ttcatctgct aaaaatggac ggatggcagc atgaggacaa acaaaggcac attcattaca
    32041 catcgtacag cgatcactaa tccactcagg tacttctaaa gcgacgcccc gcttttccat
    32101 agaagccgtt cctaaaggca tttcgccagt tgtcattcca tttgctacaa gtgtaccaac
    32161 agagagttga tttccttcct gtgcattcac tggttcaagt atttcttgta tataaccagt
    32221 gttcctttta ttgatttttt caggcatctc caacgttctc caatggtctg ggatcgcgac
    32281 ttgatgaagc agttcggtcg tccgatcgat tgcttgtatg tttttttcta cgatagccat
    32341 tgatttatgt ccatagcttt tcagggcttc ttcttttagc agcggaagca cctcatcaaa
    32401 aggaatgatg tcagctaatt taaaaaaagc cgtttccatt gccgtattga tccggcgtcc
    32461 caaaccgact tcttgggcca atctcatggc attgatcgta tagaatctaa tgttattttc
    32521 agccaagtaa cgttttaatt ttaagggcag atgcgtttct aattgttcgt cgctccaaag
    32581 agtattcaat aaaaaaatgc cacctggctt caatcctttg accaagtcat agctatgcag
    32641 gtaagctggt gtgtgacagg caacaaggtc cgcatgctcg acaagatacg ctgaacgtat
    32701 cggcgtatct ccaaaacgaa gatgagagac agtcagaccg ccagattttt tcgaatcata
    32761 ataaaaatag ccttgcgcat atttatcggt gtgatctcca ataatcttga tagctgattt
    32821 atttgctcca actgttccgt cagatccaaa tccccaaaac ttggcctgga atgtttgcgg
    32881 ttctgtcaaa tccagacttt cctttggttc caaagattga taagtcacat catccacgat
    32941 ccctatggta aaccgttttt tgcgtatgct tgggtctttt ttcaattcat caaaaactgc
    33001 tgagatttgg tcgggagtca catctttaga ccccaatccg tagcgaccac caataacagc
    33061 cgggcgaagt tcagaatcat ataacgcact ttgaacgtct aataagagag gttctccacc
    33121 tgcccctggt tctttacttc gatcaagaac agcaattgtt tttactgtat gaggcaactt
    33181 ttgcacgaat gtttccactg gaaaaggacg ataaagatgg atattcaaaa aacctgtttt
    33241 ccgtccgttt gcattcaagt aatcaatcgt ttgttcaatc gtctgtgcaa ctgatcccat
    33301 agaaacaatg acttcttccg catcgtctgc tccgtaataa gtcaccaagt cataagaagt
    33361 tcctcgtaat cggttgatct ctttcatata tttttgaacg atttcaggta atttatcata
    33421 ataggggttg atcgtttccc gttgttgaaa atggatgtct ggattctggt tcgtaccact
    33481 aacagtcgga tgattgggat tcatccctcg tttacgaaat gaagctaatt tctcctgatt
    33541 gatcagagga gctaattctt catattccaa aacttcgatt ttttgaatct cgtgactcgt
    33601 acggaatcca tcaaagaaat tcaaaaaagg tacttctcct tcaatagaag caagatgggc
    33661 aacaggtgct aaatccatca cttcttgtac actgctttca gctaacatag caaatcctgt
    33721 ttgtcttgca gccatcacgt ctccgtgatc tccgaaaata ttcaacgcat ttgtcgtcac
    33781 tgcccgacta gcaacatgga aaacacttgg cagtagttcc ccagcaatct tgtacatatt
    33841 cggtatcatc aaaagcagcc cttgagaagc ggtatatgta gttgtcagtg caccagtttt
    33901 caatgatcca tggaccacac cagctgctcc tgcttcggat tgcatttcta ctacttttac
    33961 tggttgaccg aatacatttt cttttcctgc tgctgaccat tgatctacta attcagccat
    34021 ggtcgaactg ggtgttattg gatatattgc agcaatctcg gtaaatgcat aagaaatata
    34081 tgcggctgcc gtattgccat ccattgtttt catttttcgc atgattcatc atccttgttc
    34141 atattctctc ttattatctg ttccattata acgtaaaaca ttccattttt tcttttattt
    34201 ataaaaatat ttcacatatg tctatttgaa ttggtcatct aatttttaga caaacagtct
    34261 aaatcgttgc ctttttttgt cagacatgta taaactataa gcgaaagagg tgttttaaca
    34321 tggatatatc agtgattgat gcaacgaaaa caaatacaca aaaaggaatc ctttatggtt
    34381 catcgaatgc tcctaaaaaa atggtcgaat tcatcaacct atcttgtccg tattgccgac
    34441 aatggtttga agaatcttat gagctattag aagaagcagt ccaaagcggg caattgcagc
    34501 gagtgatcaa attgttcgat aaagaaaaag aaagcttgca gcgaggaaac gtgatgcacc
    34561 ggtatctcac aatcagcgat ggtcaaaaag cgatcaaaga gatcaaacaa atattcgata
    34621 ctcaagacga gtggaaacat ctttctctcc aagaagtagc ggattttgca gtagataaac
    34681 taaagctgac agaacaaaaa gacgaacagc tgagccaagc tgtcatcaac gaagctgagc
    34741 aagcacatat tcgatttgtt ccgactgtca tcttgggtaa agaaatcttt gatgaatcta
    34801 tttcaataga ggaattaaaa gaattgatcc aaacaaaata aaaaaataag gagcgtagga
    34861 caaaagtaaa gaacactttt gtctcacgct ctaaacacct ataaacggcg gaagcagaag
    34921 caactccttc ttatttcttg gagttaaacg cttctgtccc agcctcctta ttccgaataa
    34981 atggtgtcca aaagcttgca tctgccagat tatctcattt cttatgacaa gctgagcaaa
    35041 ttatctccac caaataattt attcatattt ttgacttgcg catactaact aattcttaaa
    35101 ttttcataca aaatttctca aattccctta acgagtaata tttatgtacg gtgattcaca
    35161 ctagtatccc aatcactttt taagttatcc cgatttcaat tctacttcaa aaatagttgc
    35221 ttttatgtat gctgttagtt gattagatgg tcgtttgttg agctaggtcg tttattttaa
    35281 acaagttgtt tctttagctt atacctatct cgtctaccaa acgcaagatg gcaaacacaa
    35341 atgcatacgc atctgtattt gccactttga ttcgtataaa gctaacaact cctaactcct
    35401 ctcatttaat agttgctctc ttttttattc agatacattt ttgctgatca cagcctagta
    35461 tttcaatggt tttgaacact aaaccaaatt gttccattac aacattcact ggctgatgaa
    35521 atggattaat agatttcgat tattcaaagc gattttctat tggtggattg tgttcccaac
    35581 taacaagacg gattccaaag tcattatata actcttcaag agttaaccct ttcctgttcc
    35641 caatagatgt atctctccca cgctcgatat aaaatatgga gtttcgaatg ccaagacctg
    35701 gcaaatcttt ccttgttaca gttacactgc tactaacttc cccatataaa atccagctcc
    35761 tccaaaatga taatatattt ttcgaccatc atctaactca tgtaacaata attgttgtgc
    35821 cctcttgtca ctcgccaaat gcaaacgcca cgtgcgatac catgccatgc tacctgcatc
    35881 tgcagaaaat ccatcaaata atgcaacaat tgtatcttct atcacaatcc tttccccatc
    35941 tacttcatat tctaatctaa aatcaaactc ccctcgggta atcgcaggtc ttggtggctt
    36001 aggacccaat attattctac ttaaaaccgc attcgttccc agatgaaaca cagctataac
    36061 aacaataatg attaatattt tgtttctatt tgataaattt ctcattattc gcaaatcacc
    36121 tctacattct cacatactag ttcgtaatac tcctttaata tcaaaatatc tactgatcgt
    36181 aataatattc gctatgaatt cctactactc gtataagaaa ggccaaatca tcagctttta
    36241 cttgctgatg atttggcttg tttctattta tcgtaattgt tttctaatca ccaaaattgt
    36301 taacaatcgg ttcagatatt tcccaattca ctagccttat attatattga tttaacaatt
    36361 cttcttcatt tattgtattt tgatcaagcg gaccaaattg ttctaataat tctttgtcat
    36421 gtaaaatatc ccttctagta atgcctcttt ccctttcaat gaaagaagca ctaggaaaag
    36481 tatgattata accattcaac ctatctccca tgtaataatt agcaggtcct ggaaaataaa
    36541 atatttgctt attatcacta acttctaaca gcaacactaa atcttctccg ctggctaaac
    36601 gacttgtcca tctacgatat ctccctatgc cttcgttcat tccaaatcca tcaaattcaa
    36661 ctattaacgt gtcttctatc acatgtaatt cttcattcaa ttcatactca agtctaaaag
    36721 gaaattctcc atatgtaatc tctggcaatg gtggtgctgg agttctccaa ataaatacaa
    36781 taactgtgaa tagaaaaaat ccaacgatcg atataaatgt tatgagacct gctcgaagta
    36841 aaacccgata taataaaaat gctgtatttc gctttgctat atctagaaaa ataaaaagta
    36901 ctgcaatcat aatgatagta aaagttgtaa aaaaacgcat ttttgccctc cctttataaa
    36961 catcgattct caaattctaa accaaatctt ggtagttcaa gttcaggtat aacgtattcc
    37021 aaccatacat ctcaattact atttatgaac ttatgattat cactgaataa atcactccat
    37081 ccccttgtta aaacttctac attaattaca cctggttgtt ttcgactagc taatccaatg
    37141 aacttatcaa ccatccttat ccaccaacca cgagaatctt gaccagaagt aagaagagca
    37201 gttctcaagg atgatcgagc agttccttcc agttttttaa ttaatcaggc cagttataga
    37261 taaatacttg atcttatcta tatcaggact tctatattaa gcaaaaattt atgtacaaaa
    37321 ctaaaatctt cattcagttg tctccccgct ttttatacga ttgtattaaa aacaatagaa
    37381 gcctttccaa attatgttac tccagtaaag atggaattat agtttgacgg aagaaaagca
    37441 agtataactt ttaaaaaaaa ctgatacatt cgcatctcct gcttcatgta tcagtttttt
    37501 ctttggatta aactgcttct tcacgcagtt tttccgcaaa ttcatttatt ttgcgaattc
    37561 ggtgattaat tcccgatttt gatatcgcac ctgagggaat catttcccct aattctttca
    37621 agctgacctc tggatgttga agccgaagtt ctgcgatttc ctgaagtttt tcaggaaggg
    37681 catgtagacc gacctttgct tcgatcaatt cgatgttttc gatttgttta gaagccgcat
    37741 caatcgtctt gttcagattt gctgtctcac aattgactag acgattgacc gaatttctca
    37801 tatcccgtac gattcgaaca tcttcaaatc ggagcattga gttggttgca ccaatcaatg
    37861 ttaagaaatc agcaattttt tctgctcctt ttaaataaga aatatagcca ttgcgccgtt
    37921 caagcgttcg agcatgtaga ccgtaataat ttaacatatc gcaaatgtct tggttatgct
    37981 gttcataaat agaaaatatt tccaaatgat aacggctcgt ttccggatta ttgactgaac
    38041 ctgaggccat aaaagcacca cgtaagtaag aacgcatttt ttgcgaattt cccataatct
    38101 cgtccgaaac gtgtccattg aacatcatac catccatgat atcaagatcc atcaacacat
    38161 cttttgtgcc ttgcttcaac cggacgatat agacattgtt cttttttaat ttcatttttt
    38221 tacgaacgag tagctcactt cgtacctgat aatgatcttt caaaagagaa tagatacgtc
    38281 tggcaattgc agcattttct gtttggacgt tcaaaataaa ctgctggttc acaataccta
    38341 aagagccgtt cattcgcagc aaagcagcta attccgcttt cgcatgttct cgatgaactt
    38401 ccaaaccagt tagttctttc ttcacatctg cagcaaatga catcctattc tcctcctttc
    38461 tccaacaata aattgcttaa tattttgaac cgaaaactaa acggaacaat tcttctacta
    38521 ctttgtctcc atcgtggaag acaccaccat ctttcaattc caaaaagtcg gtggaaatca
    38581 ctcgacatcc ttcatttctc aatccgttga agtcgtgtgc gacttgtact agatactcat
    38641 catagatctc tgggtccata taatcttcag gcactttttc cgtattgacc aatactgtat
    38701 cgataaaagg ctgattcaaa tgcttgtgta aaacacgtat atgatcggca tctgtgaaat
    38761 gttccgtttc ccctttttgg gtcatgatat tacagatata gacggtttca gcagttgtct
    38821 tgagcattgc ttcgccgatt tctggtatca ctaaatttgg aaggatactg gtgaataaac
    38881 ttccgggtcc caaaacgacc atgtcggcgt ccataattgc tgaaacgact tttcgagccg
    38941 ctttggcttc acttttatca tttgtgttgc agacatacac acggtcaatg gttttacggt
    39001 cgatcgcgat tttagattct cctactgcag tcgttccgtc tttaaagaca gcatgcaagg
    39061 tcaaaggagt ttctgaagaa ggataaacat gtccatctac atgcatcatt tttgctaata
    39121 attgaatcgc ctcataagtg cttccccgca tctcggaaac tgcggcaatg atcagattac
    39181 cgatcgtatg attggctaaa aactgatctt cctttttgaa acggtattgg aaaatatctt
    39241 catagaattg tggcatatcc gataacgcca ctaagacatt tctcaagtca ccaggaggag
    39301 ccatagcgat cgattctctg attgcaccgc tgcttccccc atcatctgca acagtcacta
    39361 ctgctgtgat atctgctcct tgatttcgca aacttttcag gataactggc agaccagtgc
    39421 caccgccgac cactacgatc tttggttttc ttatccggta ggttttcatt ttcacgaccg
    39481 gttcaccgtc tctttccttt tatccttgtc ccgatgtgtc acattgactt tatagttttt
    39541 ccctaaagca tttgccaaac gttcagtcaa tgccacagaa cggtgttgac cgcctgtaca
    39601 gccgattgca actgtcaagc tgctttttcc ttctttaatg tatccaggca atacagtttc
    39661 cagaagatcc aaaaatttat ggtagaatga ttgcgtttca tcaaaggaca tcacataatc
    39721 atagactggc tgatcttttc cggtcaatgg tcttagttcc gggatataat gagggttggg
    39781 caaaaaacgc acatccatca caatatccga atcgatcggc aacccgtatt taaaaccaaa
    39841 tgatacaaat tcgatccgaa atccttggtc tccctttgat ttgaatgact cattgatttt
    39901 ttcacgtaat tgtcgtggtg tcaatgtcgt cgtatcaatg atcacggaag cttgggcttt
    39961 caagtcgtct agtatcgccc gttctttacg tatcccttca gtgatcaaac cgtccatcgc
    40021 cattggatgt gctcgtcttg tttctttgta gcgagccact agttcttcgt ctgaagcatc
    40081 taaaaataag atagaagtgt cgatgaagtt cgtattttcg atttcgatca gcatgttttg
    40141 gatttcttca aagaaggaac gagaacgtaa atccactcct aaagccattt ttgtgacttt
    40201 gcctgattct ttgattaatt cccagaattt tggaatcaat cgaggcggca tattatcgat
    40261 acaaaaatag cccatatctt caaaactttg gatagctact gttttccctg caccgctcat
    40321 acctgttatg atgactaatt gcaaattatc tgtcataatt acgaactcct ctcacattgt
    40381 gcatagtaaa attatatcat tccgggcgta tcattaccag ttttcttttt cttttacaaa
    40441 aagatttgga ccaaatatct tttaggatat ttgccccaaa tccaattttt ttacttttcc
    40501 gatgctgtat ccaatgcctt gcttctttca gctagcgctc gttcaatcgt ccgatctgct
    40561 aaaatagatt gggcatcgat cactggataa tggttatctt cagcaaattc atgcatcaat
    40621 gacaattctg tgcaacctaa gataactttt tcacagttta ggcgctctac cgcttcttct
    40681 aagatttcgt aatatagctc ttgattcaaa taatccgatt ccttgatctc atgatagatc
    40741 agataattga ttttttcttg aagaacagta tcaggaatca ctgtttcgaa tccaagattt
    40801 ttgacttcgc gttcatagat tccagctttc atacttcctt ctgttcctag aatcgctact
    40861 ctccctgtcg tatgctgacg gacaagttca tttgctgctt ctcttggcat atgaaggata
    40921 ggaatatccg ttgccgcttg taattcttca aaaaagtagt gtgcggtgtt acaagtcaac
    40981 acaatgaagt tcggctgcaa taaattttgt ttctcaatat catccagcag aaacggcatt
    41041 ggattttctt ctgatcgatc taaaatataa gctgtacgat caggaactgt tgcatgattg
    41101 aataagacat aattcaaata ttcttgatct tttgtagctt tcgttcggtg gttgatcaag
    41161 cgaacaaaac tttctgtcgc catcgtgccc attccgccta aaatactgaa aaaattctcc
    41221 atgaactagc cttctttcac atgaaaatac tttttgaatc ttggaatata gttatggaac
    41281 atatatttca tcaacaacca gcgcttgatt gaccggtctt tttcataaaa cacagtagta
    41341 ccgtaacgat tttctttgat catttgctca gctagttttt tgtcttcatt ttcccgagca
    41401 tattctaaaa agattttttt gggcacaccc atccataatc ttgctttatc tgattgattc
    41461 gttccatagg tcgtttcaac aaagggtttg ttaaacactc gatcttctgt gacgaaacga
    41521 gccagattca agccgttcaa tgtcacaaag aaactgctac gaccttgtct caaattgatt
    41581 tcaaataatt tatattctcc atctctagga tcatacttca tatcgaaatt ggcaaagcct
    41641 gtatattcga ttttttccaa aaatgcctta atggtttgat agattttttc gttataatct
    41701 ggcatgatca caacgtaatt accaatcgat gctggtgtag gatcttctaa cagtggatga
    41761 ccaagacaca tcatccgaac ttgatgatct tcatctacat acgcatttag cacacgcata
    41821 ttactatcat cacccgggat aaaatcttgt acaatcattt cacttttata accggcttcg
    41881 tatatccggc ctaaaattaa atcgaactct tctctagtct ctaaaataaa agcttttttt
    41941 cttccttcaa attggacaga aagatactcc acactgtttg caggtttcaa agcaactggg
    42001 aattcaaaag gcaattcctg ttccaaatgt ccatttacta acatttcttc tctgacgatc
    42061 aatgttttag gatacggaag atcatattct tcacaaactt cataaaagct tactttattg
    42121 atcagtcttt caaataaaga ataatcaata tatggacaga taaaatattc tgacagctct
    42181 tgtttatgtt gggagatcaa ttctgcatat ccatcaccac aagcaatcaa aagatatttt
    42241 ttactcttat cagaatatct ttcttttgcc aaacgaagca tcgtctcgat aaacacaggg
    42301 tctttatcaa atcctggaat aacttctaca ttgacaattt tactgtagcg ggtaggggct
    42361 aactgatccg aagcatatgc ttggcagatt ttgccgtatg cttcattaaa agaacgtgcc
    42421 ataccataaa cgttcatgtc gcttcctaat aatataggga taaattcttc attttcaata
    42481 ctgttcatca ttatctctca cttactcata atctcttgtt tttcttttcc aactacacaa
    42541 ggaatatcat accataaaga aaaaagcaat caaaggaaac tcctccgatt gctcaattct
    42601 tttttgaacg gattgaactt ataggaagtt tttgtctata ccaatcagtg tagaaagaac
    42661 taccatgtaa tgattctaga acactgatat taataaaaca agcaaaaatt cattttttta
    42721 tgttttttta actatctcag ctatctatcc tctttatacc attatttttt tcaaatagtg
    42781 acctgtataa ctttctttta cgttaatgat ttcttctggt gttccagtcg caaggatcgt
    42841 tcctccacct tctccacctt ctggtcctaa atcaatgata taatctgctg ttttgattac
    42901 atctagattg tgttcaatca ctaaaactgt gttaccagca tctactaatc tttgtaatac
    42961 atgcaacaag cgggcgatgt catcgctatg aagtcctgtc gttggttcat ctagtatata
    43021 gaaattcttt ccattagaga ttttgtgcaa ttcactggca agtttcatcc gttgtgcctc
    43081 accaccggac aatgtcgttg ctggttgccc catcgtcaca taacctaagc caacatcaac
    43141 aatcgtttgc agtttgcgat gaatctttgg aatgtgcttg aagaattcta cagcatcttc
    43201 tactgtcatt tccaaaatat cagcaatgct ttttcctttg taatgcactt ctaatgtttc
    43261 agagttatat cgtttgccat gacatacttc acaaggaaca tagacatcag gcaaaaagtg
    43321 catttcgatc ttaataattc catccccgcg acaagcttca caacgaccgc cttttacgtt
    43381 gaaactaaaa cgccctttct tataaccccg catttttgcc tcgttcgttt gagcaaataa
    43441 cccacggata tcatcaaata cacttgtata agtcgctgga ttacttctcg gcgtccggcc
    43501 gattgggctt tgatcgatat cgatgatctt ttcgatactt tcgtagccgg aaattgtctt
    43561 gaatttacct ggcttagcag aattcttatt taatttttgc gctaacgatt tctttaagat
    43621 actattgacc aatgtactct ttccagaacc agacacacct gtgactgcta caaattctcc
    43681 taatggaaat tcaacactaa tatttttcag attgttttct gaagcaccag tgatcttgat
    43741 tgctttccca ttacctttac ggcgttcttt tggtacaggg attgattttt tccctgaaag
    43801 atatttccct gtcaaagaat tttcatcatt agctacttct tcaggagtgc ctgcagccac
    43861 gatttgtccg ccttgttctc cggccccagg tccgacgtca atcagataat ctgctgccca
    43921 catcgtatct tcatcatgtt cgactacaac gagggtgttg ccaagatcac gcattttctt
    43981 taatgagcca atcagtcgat cgttgtcgcg ttgatgcaat ccgatcgatg gttcatctaa
    44041 aatatacaaa actccagaaa gattcgatcc aatttgtgta gccaatctga ttcgctgcgc
    44101 ttctccccca gataatgttc cggaagcgcg actcaaagtt agatagtcta gtcccacatt
    44161 ttccaagaat gaaagtctgt catttacttc ttttaaaatc ggtttggcaa ttactttctc
    44221 ttgttcagat aacgttaagt ttgagaagaa atctactgct ttttgaatag acaactcatt
    44281 tgtttgcccg atattcgttc cattgatttg aacagataga gcttggggat tcaatcgata
    44341 acctttacat gtctgacaag ttaattctgt catataaaga cgcatttgct cacgcgtgaa
    44401 atcactgttc gtatcatgat aacgacgctc gatattcttc aatacccctt cgaaagtcgt
    44461 ttctacatcg cgaacgccac caaaatcatt ttcataatgg aaatggaaaa gatcatctgt
    44521 cccattcaag atgatatcct gatgctcttc cggtaattct tcgaatggtg tatctaaatc
    44581 gataccgaaa ctttcacaag cctgcgctaa catttgagga taatactgag agctgattgg
    44641 attccatgga acgatagcac cttgactaag tgtcttcgtt ctatctggaa tcactaaatc
    44701 ttgatcgact tctaatttga cacctaaacc atcacagtct ggacaagctc caaaaggcgc
    44761 attgaatgag aacagtcttg gttctagttc gccaacagta aatccgcaat aaggacaggc
    44821 atagtgttcg ctaaacaaca tttcttcttc accgatcacg tcaaccagag catagccgtc
    44881 tgccaatcgc agagccgcct ctaaagaatc aaatagacga gaacgaatcc cttctttcac
    44941 aatgatccgg tcgatgacga tcgcgatatc atgttttttg tttttctcca gctcaggcac
    45001 ttcacttaaa tcatagatct caccatcgac tcgaacgcgg acgtatccct ctttttggat
    45061 tcgttcgaat atttttttat gttgtccttt tttctttacc acgatcggtg ccaacacttg
    45121 gatctttgtg cgctcaggca aagccattac ttgatctgcc atttgatcta ctgattgact
    45181 ggtaatctcg atatgatcgt tgggacaaat cggatgtcct acacgtgcaa acaacaagcg
    45241 taaatagtca ttgatttccg tcacagtacc tactgtcgac cgaggatttt tacttgttgt
    45301 tttctgatca atagaaatcg ccggactcaa tccgtcgatg ctatctacgt ccggcttatc
    45361 catttgtcct aaaaactgac gagcataagc agaaaggctc tctacgtatc tccgctgccc
    45421 ttcagcgtac agcgtatcaa atgctaggga actttttcca gaaccggaca aacctgtaac
    45481 gaccacgaat ttatctcgtg gaatcgttac atctatattt ttcaagttat gggcacgtgc
    45541 accgtgaatc gtaattttat cgttagccat gttgtcctcc tactttgaag cttttaattc
    45601 taaaatcata tctcgcacat tagcggctgt ttcaaaatcc aatgctttcg ccgcattttt
    45661 catttctttt tctaatttca tcaataacgt atctttttct tctttcgtca tttcttcata
    45721 cgagacttgt accgcttctt ttgtatcatc ttccgattct ttcgtaatag aaatcaaatc
    45781 tcggatttct ttaatgatcg tttttggaac gatcccatgt tcttcattgt acttctgctg
    45841 aatcgtacgg cgtcttgatg tttcatccat cgctagacgc atagaatctg ttactttgtc
    45901 tgcgtacatg attactttac cttcttcatt ccgagcagct cgtccaatcg tttggactaa
    45961 ggaacgttca ctacgtaaaa agccttcttt atccgcatcc aagatagcaa caagggaaac
    46021 ttcaggtacg tctaatcctt cacgaagcaa attgattcca actaacacat caaattcacc
    46081 taatcgaaga tctcgaatga tttctgttcg ttccagcgtt ttgatatcac tatgcaaata
    46141 tttgactttg atccctaatt ctttgaaata atctgtgagg tcttctgcca tttttttcgt
    46201 caatgtcgtt acaaacaccc gctggtcttt ttcgactcgt tcatttattt cgcctactag
    46261 atcatcgatt tgtcccatga ttggacgaac ttcgatcact gggtcaagca gccctgtcgg
    46321 acggatgatt tgttggataa cggtatctgt ttgttcatgt tcatatggtc ctggcgtcgc
    46381 agagacataa atgatctgat tgacatgttt ttcaaactct tctaaccgaa gtggccgatt
    46441 gtctaaagca gatggcaaac ggaaaccgta atctactagc atctgttttc tcgctcggtc
    46501 accattatac attcctctga cttgaggcat ggttacgtgc gattcgtccg ccacgatcaa
    46561 aaaatcttca ggaaagaaat ccaatagcgt ataaggcggt tcaccttctt tacgtccgtc
    46621 catatgccga gaataatttt caattcccga cgtgtagccc atttctaaca tcatttcaat
    46681 atcgtaattg gttcgctgtt ctaaacgttg agcttccaga agtttgtttt catttcgcaa
    46741 gacagttaaa cgctgttcta attccgcttt gatattcgct acggcatgct ccatatgttc
    46801 atcattcgtc acaaagtgag tcgccgggaa gattgccacg tgttccgttt ctcctaaaac
    46861 ttcacctgtc agtgcattga cttctcggat acgttcgacc tcatctccaa aaaactccac
    46921 acgtaaggca cgttcatctc ttgaagccgg aaaaatctcc acgacatcac cgcgcactcg
    46981 aaaacgaccg cgctgaaaat ctatatcatt tctttcgaat tggatactga ctaagtcacg
    47041 aatcaactga ttgcgatcta gttctgctcc ctgtcgaatc gaaactactt gtttttggta
    47101 ctcaaaaggc gaacctaatc caaaaataca tgaaacggat gcaatgacga tcacatcatt
    47161 tctttctaat aatgaactag tagccgaatg tcgtaatttg tcgatctcat catttacact
    47221 tgaatctttt tcgatatacg tatcgcttga aggcacataa gcttcaggct gataataatc
    47281 ataataagaa acaaaatatt caacagcatt gttagggaaa aattccttga attccccgta
    47341 cagctgacca gccaatgttt tgttatgggc aataattagt gttggtttat tcactttctc
    47401 aataagattg gaaatcgtat atgttttccc tgttccagtc gcacctaata agatttgtgc
    47461 ttttttcccg ccgactacgc catccactaa ttggttaatc gcttcaggct gatcaccagc
    47521 cggttggtat ttcgagacaa gctcaaagtg tcgggaggta tctctctcaa tcattcttcg
    47581 tcctcctttt cattcattcc ttttcatttt acgaccaata aaaagaaaag gaaagcaaag
    47641 gcattcctta ctaaaaaatt ataccacacc gaacatactt tcgccacttt tttatcttat
    47701 tatgcatgtt acgttttata acataaaatg ttagaaactc gcattttttt cattttttcg
    47761 agcttacagt cttgtctgat tcagcacttt tcgcctataa taataaaata ttggaaattt
    47821 aggaggttga ttatgaaaag aaagcattta tttcactcat ttgttttctt aatgacagga
    47881 cttgtgacac ttgcgtttgg acatgttgca caagctgaca acactacaac ggcagagtca
    47941 agcacaagcg agacaatgca aaaaatagag cccaaaaaag acgtatacgt aattgccagt
    48001 gactctgctt ttgctccatt tgaatttcaa aacgcaaagg gcgagtacga aggcatcgat
    48061 gtaaaattga tggaaaacat cgccaaactg caaggattta acatccgaat ggactaccgt
    48121 ggtttctctg ctgctttgca agctttggaa tcaggacaag cagacggaat gattgccgga
    48181 atgactgtaa cagatgaacg aaagaaaagc tatgattttt ctgattctta ctttgatagc
    48241 gggatccaat tagctgtcaa aaaaggagat acttcgatca agtcttatga agatttgaaa
    48301 ggaaaaacag tcggagcaaa agttggtacc gaaagtgctg aatttttaag cgaccatgaa
    48361 tctgaatatg gctattctat caaatatttt gatgctgctg atcaattgta cgacgggtta
    48421 aaagttggcg caatcgatgc aatgatggat gattatccgg taatcggtta tgcgatcaaa
    48481 caaggacaag aattggaaac accgatcaaa aaagaatctg gcggaaaata tgcatttgcc
    48541 gttaaaaaag gacaaaatcc tgagcttctt aatatgttca atgaaggatt gaaagaatta
    48601 aaaagaacag gcgaatacga taagattatt aaagaatatg tagcggatgg ttcagaaact
    48661 tccagtgagt cttccactgc tgatgaatca acatttcttg gacttatcca aaacaactat
    48721 caagcattgt tgaaaggact ttggagaaca attctcctaa ctttgatctc ctttgctttg
    48781 gctatagtaa tcggtgtgat ttttggatta ttcagtgtag caccaatacg cgctcttcgc
    48841 acaattgcta gtatttatgt cgatatcatc cgaggaattc caatgatggt tcttgcgttc
    48901 tttattttct ttgggttgcc tggtatcgtt ggatttacga ttcctgactt ccttgctggt
    48961 gttatcacct tgactcttaa cgcaagtgct tatatcgcag agattgttcg aggcgggatc
    49021 aacgccgttc ctgtcggaca aatggaagct tccagaagtt taggtctttc ttataatcgg
    49081 acaatgcaaa aaatcatttt gccgcaagcg atacgcatca tgatcccatc attcgtcaat
    49141 cagttcgtta tctcattaaa agacacaacg atcatttccg caatcggtgt agtcgaattg
    49201 ctgcaaactg gtaagatcat tgttgcacgt acaacacaaa gtacgtatgt ttacttgatc
    49261 attgcgatca tgtacttgat tttgatcaca gcattgacaa aattagcaaa agttttagaa
    49321 aagaaggtga agtaagatgg cagaaaaaat cttagttgag catttagtca aaaaatacgg
    49381 cgataacaca gtattaaacg acatcaatgt gtccatcaac gaaggagatg tcgtctgtat
    49441 cattggccct tcaggttcag gaaaaagtac atttttgcgt tgcttgaatc gtttagaaga
    49501 accttctgac ggtgaaatta ttattgacgg cgcccacttg atggataaaa gcacagatat
    49561 caacttggtg agacaacata tcggaatggt attccagcac tttaacctat tccctcatct
    49621 ttctattttg gaaaacatca ttttagcacc gatggacctg aagaaagaat ctagagaaga
    49681 agcagagaaa aaagcaatca aattgctgca gacagttggt ctagaagata aaaaagatat
    49741 gtatcctgaa atgctttccg gtggacaaaa gcagcgtgtg gctattgctc gtgctctagc
    49801 gatgaaccct gacatcatgc tttttgatga gcctacatct gcactcgacc ccgaaatggt
    49861 cggtgatgtc ttgaacgtca tgaaaaagtt agcagaacaa ggtatgacga tggttatcgt
    49921 tacgcacgaa atgggcttcg ctaaagaagt ggccaatcgt gtcatgttca ttgacgacgg
    49981 aaacttttta gaagacggaa cgcctcaaca aatctttgaa aatccgcaaa atgaacggac
    50041 aaaagatttc ttgaataaag tattaaatat ttaataaaaa ccaaaaatgc ccggtcgctt
    50101 catgacgacc gggcattttt agtttcttgt tttttcactt aactgtacca gccgcatcgc
    50161 ttgttctcct gcacaaacaa gcaatcgctc tacctgttcc atcgcataag aaagtaccat
    50221 cggttcattg acgatatcaa gtacatagtc gataccacct tcagaaacta tgatgattat
    50281 cttattctaa agctggcttt tttgttggta caaacatgac aactacaaaa aaaccaggcg
    50341 aacgtatgat tgttcgtctg gtttttaatt attaatgact tagcgttttt actagaaata
    50401 gaaaatttgg gcaagtctac tttatatttt tactagaaaa actacttccg ctttttcccg
    50461 ccaaaagcat ctttaaactt atcgaagaaa ccgtcttctt gctgttcttc aactttgatg
    50521 ccgccagctt cagcgaatgc acgtaatgct tctctttgtt gttcatttag atttttcggt
    50581 gtgatcagtt ttactttgac atgttggtcg cctgttgcac caccacgtaa cttcggagcg
    50641 ccttttccac gtaaacggaa gttcgtacct gtctgtgttc cagcagggat cttcaatttc
    50701 acatctccat ggacagttgg tacttggact tcatccccca aagctgcttg tacaaaactt
    50761 aaaggcaatt catagtaaat ttcagaacct tcacgatcaa agatatcact ttcttctaca
    50821 cgaaaaacca catacaaatc accaaatggt ccgccgtttt ctccagcttc tccttgacca
    50881 gctaaacgca tttgttgtcc ttcttctaca ccagctggta cattcacttt aactgaatgt
    50941 gctttcttct catgtcctga gccatgacag gttggacaag gttctttgat ttctttccct
    51001 gtgccatgac aaacatcaca agtttgacgg ctcatcactc gtccaagtgg cgtttggcgt
    51061 tctacattga tcgtacctgc accatgacat ttatgacaag tagttggttg tgtgcctggt
    51121 tttgcaccgt ttccaccaca agtatgacag atttcttccc gattatactt gatttctttt
    51181 tctacaccaa aaatcgcttc ttcaaaagac aagtcgaccg tatattgcaa atcagcacct
    51241 tgacgaggcg catttggatc gactgaccga ccgcctcctc caaagaaaga gtcgaagata
    51301 tcttcaaaac caccgaagcc gccaccagag aagccgccga atccaccaaa tccgccacca
    51361 gctccaccag caccgtaatt tggatctgtg cctgcatgcc cgtattggtc gtatgcagcg
    51421 cgtttttgcg ggtcacttaa aatctcatat gcttctgata cttctttgaa tttttcttcc
    51481 gcatcaggtt ctttattgat gtcaggatgg tattttttcg aaagcttacg ataagctttc
    51541 tttatttcat cttctgatgc tccttttgag agccctaaga cttcataata atcacgtttt
    51601 gttgccatag gctttcctcc tattggttct catcattttc acgtcgcttg ttatcatatc
    51661 atattttgtt tgaaacaaaa accgatactt caaactattt ttcaaaagaa aaagccaaag
    51721 tagcaacttt ggccttctct cgaaattcag tgaactgatt atttatcgtc gccatcgact
    51781 tcttcaaaat cagcatcaac gacatcatct gctccgcctt gagctgcttc tggattttct
    51841 tgagcttgtt gttgtgcagc ttgttcgtat agtttaacag tcaagttttg aacgatttcg
    51901 ttcaatgcat cgcgtttagt tttcatttcc tcgatgttgt ttgcttcaac agcggctttt
    51961 aattcgtcac gagctgtttc tgcttttttc acttcttctt cgtccacttt gccttctaat
    52021 tctttcaatg ttttgtccac agagaatagc aatgcgtcta catcattacg taggtcaact
    52081 tcctctttac gagctttatc tgcttcagcg tttgcttctg catctttcac catacgttcg
    52141 atttcttcgt ctgttagacc tgaagaagat ttgatcgtga ttttttgttc tttttgtgta
    52201 cctagatctt tcgcagatac atttacaatc ccgtttttat caatatcgaa tgttacttcg
    52261 atttgtggaa taccacgagg agctgctgga atatctgtca attggaatct tcctagagtt
    52321 ttgttgtcag cagccattgg gcgttcacct tgtaatacat ggatgtctac tgctggttga
    52381 ttatctgccg cagttgagaa tacttgtgat ttacttgttg gaatcgttgt attgcggtca
    52441 atcaatttag tgaacactcc acccattgtt tcgataccta atgacaatgg tgtaacgtca
    52501 agtaaaacaa catctttcac atcaccagtg atcacgccac cttggatagc agcacccata
    52561 gcaactactt catcagggtt aactgatttg tttggttctt ttcctgtttc tttacgtact
    52621 gcttctacaa ctgcagggat acgagtagat ccaccgacta aaataacttc gtcgatttct
    52681 gattgtgaca gacctgcatc ttttaacgct tgacgtactg gtacttttgt acgttctact
    52741 aagtcagctg ttaattcatc aaattttgca cgtgtcaatg tcatttccaa atgcaatggt
    52801 ccagattctc ctgctgtgat aaatggcaag ctgatttgtg tgcttgatac gccagataaa
    52861 tcttttttcg ctttttcagc agcgtctttc aagcgttgta aagccatttt gtcttgagac
    52921 aagtcaatgc tgttttcttt tttgaattct gctaccatgt agtcgatgat tttgttatca
    52981 aagtcgtccc cacctagatg gttgtcgcca gctgttgaca atacatcaaa gacaccgtct
    53041 cctagttcaa ggatagatac gtcaaaagta ccgccaccaa ggtcgaatac taagattttt
    53101 tcgtcgcgat ctgttttatc taaaccatag gcaagagcag ctgcagttgg ttcattgacg
    53161 atacgttcta cttctagacc agcgatctta ccagcatctt ttgttgcttg acgttgtgca
    53221 tcattaaagt aagctggcac agtgataact gctttttcga ctttttcacc taaatagtct
    53281 tctgcgaatc ctttgatata ttgaaggatc attgctgaga tttcttgtgg tgtatatgat
    53341 ttaccttcta catctacttt gtaacctgct tcaccgatat ggcgtttgat tgatgaaatc
    53401 gtatttgggt ttgtcacagc ttgacgtttt gcaacttccc ctacttggat ttctccattt
    53461 ttgaatgata caacagaagg tgttgtacgg tttccttctg ggttagcgat gatttttgct
    53521 tcgtttcctt ccaatactgc tactgcagag ttagtcgtac ctaagtcaat accgataatt
    53581 ttactcataa ttaacgtctc cttctttaat tgaaagtgta agttttatat ccgcacaatt
    53641 attgtgcaac aatgaccata gttggtctta acacgcgatc atgtaatttg tagccttctt
    53701 gtagtacttc tacaatcgta tcggctggtg tatcttcagt cgctggaaca gtttgtactg
    53761 cttgatgaag attaggatca aaggcttctc ctttagcagg gattttttca attccttctt
    53821 cttccagtgc gttacgcaaa ctttccaaga ccatttccac gccttttttc aaacctgccc
    53881 cttgatcatc tgacacttct gtggctaatg cacgttccag gttgtcaatt gatggaagta
    53941 attttttggc taaatcttgt gaacggtatt tttgtaattg ctcacgctca ttttttcctc
    54001 gatttgccat atttgcgatt tcagcacgag cccgcaagta tttatcttcc atttcttcta
    54061 actcagcttt taatttcgct gtttcgaatt cttccgcttc agcttctgaa atacccgaat
    54121 cttctgcagc ttcgacatcg atttccggtt ctggctgagc atctgtcatt tctttatcta
    54181 gttcttcttg attttctttc acgatacagt cttcctttca ttattcagtc agctgtttga
    54241 ttgctcttac taagaaagag agaacgatca gctgcaaata gcttcgttat tcacattacg
    54301 agtttgacaa gtcaagcgag cggtagtaat cagcaagttt agctgctagt tctcttcgat
    54361 aaacatcgac tagaccgaac atccgagaat aaggcatact agtaggccct aacaaggcta
    54421 ttgtacctct tccatgacca tctatctcgt aacttgcttg aatcatactc atattctgca
    54481 ataaatcatt gcctaattca gcaccaatct tgatatgaat attgctatcc atcggagtga
    54541 tcatttgtgt aagtccgtct gagtctttca taaaagaaaa catcgatttg aactgatcaa
    54601 gatcttgacg gggctcataa tccaagagat tcatttgtcc actgacataa accttttctt
    54661 caaaaacatg accaagaacg gtgtcaaata actgagacat cccttctgtc gtctggaaat
    54721 atttatgaag aatcatcgga atctccgtac gaagtttatg gtacaccgtc atcagaggat
    54781 ctccgactag acggtcgttt acaatctgta ccattttttc tagttcctga cttcccatat
    54841 attcgggaag agtgaacact tggctttcta cattccccat atctgttacg acgatagcaa
    54901 taatctgtcg attattcaag ggtacgatac gaaagccagt cagtcggcgg tcttttattt
    54961 caggtcccaa tgaaaaagtc gtatagcttg tcagctcaga taatatctca gcagattgct
    55021 taataatctc attgatttca tgaaattctc tgcctagaga ctgacgaatc agcagcaaat
    55081 catcttctgc tacgcgagca ggttttaaca aatgatccac gtaataacga tagcctaaag
    55141 cagatggaat ccgcccagaa gaagagtgag tcttcaacag caatccttca tcttctaatg
    55201 ctttcatatc attgcgaatc gttgctgagc tggccttgac tccctcagac attaatgttt
    55261 tggaaccaac gggcacgcca ctactcgtat agtgttggat gatcagacgt aaaatgtcgt
    55321 tttgtcgctt tgttaacatc ttcctcacct ctcattagca ctcatatatt ctaagtgcta
    55381 attcacttat aaatatagca agaagccctt ctcctgtcaa gagaaagact ccatttttta
    55441 gcactctaaa cacaagagtg ctaattctaa ataattgatg taaatccagt tatatcaata
    55501 cttcaatacc tcattgtcaa actttctaaa ctttttcttt tcaagtttcg ataaaaaaca
    55561 aatattcttc aaactatttt tcaaataaaa agcgctcaaa cacattgttc cccaaaaaag
    55621 tccctcttgg tgtcaaataa acacggtctt cttcattaac aagtaaccct tcttgctcta
    55681 attcccttag cacatcaccg tacaccgatt caattgtttt ttgatagcga tcatagaaat
    55741 gttttttagt aatccctgtt ttttttctta gtcctaagaa catttcttct tctatttgat
    55801 tttttcgtgt caatttttct tcttccaata ccgggcgttc atttgctcgt aatgggcgta
    55861 aatagtgttg gattggtcct ttgttgcggt aacgaatatc tccaagataa ccgctagcac
    55921 cagctccaaa cccaaaatag tggtcattgt tccagtacat gaggttatgt tgagattcat
    55981 gtccggagag cccaaagtta cttatttcat attggtgacg tccatgcttt tccattgctt
    56041 caatcgtctc ttcaaacatt ctggtttccg tttcctgatc aggcagttcg agtcttcctt
    56101 gtcgtaccca gttcatgaac attgttttgt tttcgaggat caatgagtac aaagaatagt
    56161 gaggcagatc caaagccaaa gctttttcca aagtatcccg atatccttct agtgtttgtc
    56221 ccggtaatgc ataaatcaaa tcgatgctta cattggtaaa attttctgct tctaaaaatt
    56281 gcattgtttg atacacatct tctgcagtat gtttacgtcc aatttttttc aataagcgat
    56341 tgtcaaatgt ctgcactccc atggataaac gattgacgcc atagtttttc atcacttgaa
    56401 gtttttcacg ggtcaaatca ccaggattcg cttctacagt aaactcatta ccattttgaa
    56461 aaggcaggat ctctctagca cctgataata gtcgatcaag ttgtttagca gataaagaag
    56521 ttggtgttcc gcctcctaca taaagtgttt ccaaattcgc tgtaggatga ctttccagca
    56581 ttaatctcat ttcattcagc agcatttcca catactcatc tactggctgc ccttctaaaa
    56641 aaactttatt aaagtcacag taatagcaga tatgctcgca aaatgggata tgaatgtacg
    56701 cagaaacctt gttttctgcg ggatttattg tttctatcat tgaaattctt tctccttacg
    56761 tcgaaaagtc gtgcctgttt tcgaccttct taaaatcgcg ttcaagcacc aactcattga
    56821 aataagccgc atatttagaa tcaaggaaac ctagctctaa aaatgcttgc ttattgtcat
    56881 cggagctaca cggtgctttt acagcctctc gataaacggt aatcgcaaag ctgaccttcg
    56941 gcattaagtc aaaatctgca aaaatatgaa aagctatttt cacagattcc tccttaagtg
    57001 ctcagaccac aacgctttgc ttacaacctc tcatatacgc atactaaacg tatttggcga
    57061 acaaatccac taaaaaatcc aagaatcgac ttcttggatg atttttttac actcatgatt
    57121 cttcctaaac aattttgtcc ttattttatt cataaaaaaa tctagtcaaa tctctcactt
    57181 caagagtttt aactagattc caattattat ttttttgtaa aaataaacag tctcttaatc
    57241 atagacattt cttcggtgcc ctacttgaat agcgataaca acgaattgat tatcttctat
    57301 ctctaccaat acacgtattt gccaatcaga gctgcttctg aatttcgggc gtgaatttta
    57361 aacggcaggt cattccagcc caccaaattc attaaggatt cttccatgaa tactgttgtt
    57421 ttcccacttc ttatccaatg tttatgggca atttcagccg tttgacgatt gtattcatct
    57481 tctagttgtt ccatcgaata gttttttaga agttcagata aagtaatccc ataaaattca
    57541 gccatataag aaagccattc ttttttggcg tcacttacac gaaccgtaat catagcccta
    57601 taattgcctc tcttaaagaa tctcatgcat ttcctgatgt gattttagaa aaatttactt
    57661 gctaaaaaac tgtgaaaatg taggagctag catccttgta gtaaatgatc gagctcttta
    57721 ttatacatta aaaaagttca gaaattttga tgaagtagcc aactactttt tcatgagtaa
    57781 agcaacacta gaaaaagaat cctccagtac ttagaattta ctggaggatg tacttttgaa
    57841 gaagcctata ggttatttaa aaaatattac tacaggaagg aacgagaatt acttcgttac
    57901 ttataataga acttagtctt atatagaagg catagctcac attagcttat ttgtcctata
    57961 aaaataaatt acggtatttt agaaaagcta ttttctcttt tttcaacgat cttcagcaat
    58021 ttttctttat cgtcaggtaa ctcaagttca tgaattctag catgacaatt gggacataac
    58081 gcgatacagt tttcaatcac atcttttcca ccttgcgaca aatattctat atgatgacaa
    58141 tgtaaataag gtacaccgtc ttttataaat ggcccttttt cttggcaaag ttgacaaata
    58201 ccttttgcaa gtcgtttaac atattcagat acgaagggat tacgtttata tatttttgtt
    58261 tttgagaagc aaaactcctc ttcgttaaca tattttgcag ccattttttt attataatta
    58321 ctaacttttc ttgcttcttc ctctaattta gcattagata aattacgaat atgtctagtt
    58381 tgattttctt cctggttata tatctttatc ttcaatcaat tgctcttgat ttattaaagc
    58441 taatggaaat ttatatactt ttctgctgtt gcccgaacta tctttttcat taacagtata
    58501 tatttgtcct gctaaatata cttctcccag atatgtgtgt tcttttggtt taaatgtttc
    58561 aaataaatga atctttacaa aattactttt tgaattggct aaaactttat tttgattctt
    58621 atccaatact tgatcccctt ttagccccat accggtatat tgtaaaatac catttaacca
    58681 cttatcttta taaacattac ttcctgtttg atcactaaac agtacaagag tattagtttg
    58741 atgagatctt ctcattccac cctgtttgga acatttaaac ttctctgaaa tttctttgtt
    58801 agtatatact ctaccaactt ctaactcacc taatatgttt ttagaccaat tttctcgcca
    58861 tgtaataatc ttatcaagat taaacatttc tccctctttt ggcatgccat ttctaattaa
    58921 attagctaat acaccagtag aaatatctaa tttttttagt aattctttct tagtaatcaa
    58981 tgttggattc ataacaatgc agctcctaat gatatcaata taattgcatt agataacttg
    59041 aaaaatcgaa aaaaactcat tttgtgtcaa gttatctgta atatataaaa atatcattat
    59101 tatacctcat ctatgtgaat ttttactatt cttaccttag catattcttt ataggtatat
    59161 cagagctaat caaactaaaa aatacccgaa gtcttcttat gtaaaaacac cggttattac
    59221 ttaaatatga cttaggtaaa acaatgagtg atccaattat aaaattttat ggcgaataat
    59281 ttagtaagcg ctacatattg aatgatttag aaattttgaa aaagtagcta gttcctttta
    59341 taccagaaaa gtaaacctaa taaaaaacac aactaatttt tagctgtgtt ttt
//
LOCUS       DACDYI010000008        58159 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.008, whole genome shotgun sequence.
ACCESSION   DACDYI010000008 DACDYI010000000
VERSION     DACDYI010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 58159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.008"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(524..1078)
                     /locus_tag="IWM52_000466"
     CDS             complement(524..1078)
                     /locus_tag="IWM52_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAP9972746.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(1204..3132)
                     /locus_tag="IWM52_000467"
     CDS             complement(1204..3132)
                     /locus_tag="IWM52_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP9972747.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(3438..4154)
                     /locus_tag="IWM52_000468"
     CDS             complement(3438..4154)
                     /locus_tag="IWM52_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP9972748.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(4155..5978)
                     /gene="glpO"
                     /locus_tag="IWM52_000469"
     CDS             complement(4155..5978)
                     /gene="glpO"
                     /locus_tag="IWM52_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAP9972749.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(5992..7488)
                     /gene="glpK"
                     /locus_tag="IWM52_000470"
     CDS             complement(5992..7488)
                     /gene="glpK"
                     /locus_tag="IWM52_000470"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAP9972750.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(7624..9084)
                     /locus_tag="IWM52_000471"
     CDS             complement(7624..9084)
                     /locus_tag="IWM52_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9972751.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(9074..10369)
                     /locus_tag="IWM52_000472"
     CDS             complement(9074..10369)
                     /locus_tag="IWM52_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9972752.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(10701..11378)
                     /locus_tag="IWM52_000473"
     CDS             complement(10701..11378)
                     /locus_tag="IWM52_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAP9972753.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(11408..12499)
                     /locus_tag="IWM52_000474"
     CDS             complement(11408..12499)
                     /locus_tag="IWM52_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAP9972754.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(12600..13034)
                     /locus_tag="IWM52_000475"
     CDS             complement(12600..13034)
                     /locus_tag="IWM52_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9972755.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(13618..15075)
                     /gene="ascB"
                     /locus_tag="IWM52_000476"
     CDS             complement(13618..15075)
                     /gene="ascB"
                     /locus_tag="IWM52_000476"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP9972756.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(15072..16967)
                     /locus_tag="IWM52_000477"
     CDS             complement(15072..16967)
                     /locus_tag="IWM52_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9972757.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(17025..18800)
                     /locus_tag="IWM52_000478"
     CDS             complement(17025..18800)
                     /locus_tag="IWM52_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9972758.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(18850..20346)
                     /locus_tag="IWM52_000479"
     CDS             complement(18850..20346)
                     /locus_tag="IWM52_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9972759.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(20540..22162)
                     /locus_tag="IWM52_000480"
     CDS             complement(20540..22162)
                     /locus_tag="IWM52_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP9972760.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(22611..23924)
                     /locus_tag="IWM52_000481"
     CDS             complement(22611..23924)
                     /locus_tag="IWM52_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP9972761.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(24169..24393)
                     /locus_tag="IWM52_000482"
     CDS             complement(24169..24393)
                     /locus_tag="IWM52_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972762.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(24413..24781)
                     /locus_tag="IWM52_000483"
     CDS             complement(24413..24781)
                     /locus_tag="IWM52_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP9972763.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(24978..25688)
                     /locus_tag="IWM52_000484"
     CDS             complement(24978..25688)
                     /locus_tag="IWM52_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972764.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(25706..26203)
                     /locus_tag="IWM52_000485"
     CDS             complement(25706..26203)
                     /locus_tag="IWM52_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9972765.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(26240..26692)
                     /locus_tag="IWM52_000486"
     CDS             complement(26240..26692)
                     /locus_tag="IWM52_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9972766.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(26827..27432)
                     /locus_tag="IWM52_000487"
     CDS             complement(26827..27432)
                     /locus_tag="IWM52_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP9972767.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(27882..28502)
                     /locus_tag="IWM52_000488"
     CDS             complement(27882..28502)
                     /locus_tag="IWM52_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9972768.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(28504..29373)
                     /locus_tag="IWM52_000489"
     CDS             complement(28504..29373)
                     /locus_tag="IWM52_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAP9972769.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(29396..29803)
                     /locus_tag="IWM52_000490"
     CDS             complement(29396..29803)
                     /locus_tag="IWM52_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP9972770.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(29822..31111)
                     /locus_tag="IWM52_000491"
     CDS             complement(29822..31111)
                     /locus_tag="IWM52_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9972771.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(31138..31440)
                     /locus_tag="IWM52_000492"
     CDS             complement(31138..31440)
                     /locus_tag="IWM52_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9972772.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(31465..33558)
                     /locus_tag="IWM52_000493"
     CDS             complement(31465..33558)
                     /locus_tag="IWM52_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9972773.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(34047..34811)
                     /locus_tag="IWM52_000494"
     CDS             complement(34047..34811)
                     /locus_tag="IWM52_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAP9972774.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(34922..35791)
                     /gene="mdcB"
                     /locus_tag="IWM52_000495"
     CDS             complement(34922..35791)
                     /gene="mdcB"
                     /locus_tag="IWM52_000495"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAP9972775.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(35816..36892)
                     /locus_tag="IWM52_000496"
     CDS             complement(35816..36892)
                     /locus_tag="IWM52_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAP9972776.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(36916..37509)
                     /locus_tag="IWM52_000497"
     CDS             complement(36916..37509)
                     /locus_tag="IWM52_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAP9972777.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(37513..39189)
                     /gene="mdcD"
                     /locus_tag="IWM52_000498"
     CDS             complement(37513..39189)
                     /gene="mdcD"
                     /locus_tag="IWM52_000498"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAP9972778.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(39179..39478)
                     /locus_tag="IWM52_000499"
     CDS             complement(39179..39478)
                     /locus_tag="IWM52_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAP9972779.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(39580..41226)
                     /gene="mdcA"
                     /locus_tag="IWM52_000500"
     CDS             complement(39580..41226)
                     /gene="mdcA"
                     /locus_tag="IWM52_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAP9972780.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(41233..42144)
                     /locus_tag="IWM52_000501"
     CDS             complement(41233..42144)
                     /locus_tag="IWM52_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP9972781.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            42264..43121
                     /locus_tag="IWM52_000502"
     CDS             42264..43121
                     /locus_tag="IWM52_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9972782.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(43308..43670)
                     /locus_tag="IWM52_000503"
     CDS             complement(43308..43670)
                     /locus_tag="IWM52_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972783.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            44016..45455
                     /locus_tag="IWM52_000504"
     CDS             44016..45455
                     /locus_tag="IWM52_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9972784.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(45462..48014)
                     /locus_tag="IWM52_000505"
     CDS             complement(45462..48014)
                     /locus_tag="IWM52_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAP9972785.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPKGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYELGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(48128..49459)
                     /locus_tag="IWM52_000506"
     CDS             complement(48128..49459)
                     /locus_tag="IWM52_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9972786.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(49425..50903)
                     /locus_tag="IWM52_000507"
     CDS             complement(49425..50903)
                     /locus_tag="IWM52_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9972787.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(51208..53043)
                     /gene="tdc"
                     /locus_tag="IWM52_000508"
     CDS             complement(51208..53043)
                     /gene="tdc"
                     /locus_tag="IWM52_000508"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP9972788.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            53513..54940
                     /locus_tag="IWM52_000509"
     CDS             53513..54940
                     /locus_tag="IWM52_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9972789.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(55032..55397)
                     /locus_tag="IWM52_000510"
     CDS             complement(55032..55397)
                     /locus_tag="IWM52_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP9972790.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(55394..55759)
                     /locus_tag="IWM52_000511"
     CDS             complement(55394..55759)
                     /locus_tag="IWM52_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP9972791.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(55951..56199)
                     /locus_tag="IWM52_000512"
     CDS             complement(55951..56199)
                     /locus_tag="IWM52_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972792.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            56198..56326
                     /locus_tag="IWM52_000513"
     CDS             56198..56326
                     /locus_tag="IWM52_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAP9972793.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            56383..58077
                     /locus_tag="IWM52_000514"
     CDS             56383..58077
                     /locus_tag="IWM52_000514"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAP9972794.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
BASE COUNT        16931 a        12370 c         9789 g        19069 t
ORIGIN      
        1 ggaagaggtt gtgaaatcag cggtttgacc taagtattag agaaaaaaac gggaaaatag
       61 ctcctcatat tttgccgttt tttgagctaa taccgaaggt cagctgattg aacaccgttt
      121 aaggaagaga ttgtgaaaaa gtcgcttagt ttcgagtatt aagaaaaatt ttgtgaaaat
      181 ggatttctac atttttgcat aaatttggct taataccgag aaactggctt ttgaataccg
      241 tttaaggaag aggttgtaaa atcagcggtt tgacctgagt attagagaaa caaacggaaa
      301 aatagctgct catattttgc cgttttttga gctaataccg aaggtcagct gattgaacac
      361 cgtttaccaa gaggttgtga gcctaccatt taactccgaa tcacaagaaa taatttcaaa
      421 aacaacttct cctgtttttg gtttggttgg cttattatcg agaagtagct actgggagtc
      481 tatttatcaa aaaaaggaat gcgacatagg tcacacccct tttttatttt ctttgaaaca
      541 tctttcggta ctcgacagca tacaagacaa aagattgtaa aagaccgtaa gtgatgacgg
      601 caaaaatgac tctcacccac agcgttggta attctgggaa aagcagcaaa aggatcatgc
      661 caacatagat gacagcagtc gctactttgc cataccactt cgctccactt aatttgatat
      721 ttttagatag cccgattaat ccagctacaa acataaagcc ttcttttatc aagaatagac
      781 caaacaacag ccaaagccac ggtcgatacc atgccataga caaaatcaga aaaagttgtg
      841 tcaatttatc tgcaaacgga tcaagcacct tccctaagtc tgttacctga ttatatctcc
      901 gagcaatgac cccgtctaga taatcagaca aacctgagac aacaatgacc gctcctgcta
      961 tataaaatga ctgtctcagc acaaaataaa gataaatcgg cagcaaaaaa atacggaata
     1021 tcgataaaag attcggaatc gtttgtattt ctttacgcca gtctgtgctt aatttcattt
     1081 tatcgcccta cctcctcagc actcttttct ttttatttta gcttacacgg ggaataaaac
     1141 caatgatttc actgttttta tttttcttta cagccactga tattatgagc taatttgaga
     1201 taatcacttg ctgttttcat cgtttactct tctttcttcc tgtcgtttgc gcatttcttt
     1261 tactagtact ttatgagctt tttttgatcc tcgctctagg tcacgctgcc gtttacgtgt
     1321 tttataatcg ataaacagac tgtctaaatc gtaacctgtc gggtacagat cttctgctga
     1381 acgcaccaga cgaacacgct gacgaggcac gtggattatt tttttgtcta aatatacatc
     1441 aatcgtatct tcacctatat cttcataaac taacgcttct ttttggtatt cattgacaaa
     1501 tatccgatca ccttttgcaa agagctggcg ttctttttta ttctccttcc tatttgtaac
     1561 tgctgtctta ggaagaaatt cttttttctg tgtccgatag gctttttccg ccaagtaacg
     1621 ctctgcttga tggatcaatt tcatcgacat atccatttta tgagcaatcc acaaagcttg
     1681 gctttctcct gtttctccaa cccgcaactg atatttagga cgaagtgctt cccgatcgaa
     1741 agccatagca gccggcacaa aatcttcatg atctccggcg aatttcttga tttctccata
     1801 atgggtcgtg gcaatgatca atgccccttg ctcatacata gattccatga ttgcgatcgc
     1861 tagtgcagcc ccttcatttg gctctgttcc actgccaatc tcatctaaaa gtaccaacgt
     1921 attccttttt atctttttca aaatagcagc gatgttttgc atatgaccag aaaatgtact
     1981 caacgcattt tctaaatttt ggtggtcacc gacatcgacg aaaatctcat caaagacagc
     2041 aagctctgtc ccttcttttg ccggaacttg cataccgaac atcgccatca aagtcaataa
     2101 accgactgtc ttcaacacga ctgttttgcc tccggcattt gctccggtga taatcaagcc
     2161 tcgatagtct ttaccgattt caacatccaa tggtaccgca tgctcaggta aaaatggatg
     2221 tctgccttgc ttgataatga tatgttccga tttattgatt ctaggtgtgc ttccattgat
     2281 ttctcggcta aattttgccc gagcaaagat gatatctaac accgtgatcg tttcgatcaa
     2341 taaatcgatc acttcttcgt tttctgcgat agcaccagtc aatgccgcca ggacttgata
     2401 ttcttcactg atttcttcag cttttaacaa ctgatagtgt tcattcaatt tagaaatcac
     2461 agttggttca ataaaaaccg tcgttctttt atttgattca tcgataatcg ttccatctat
     2521 tttattttta tagctggctt tgataggaat cgtatagtat tcgccttttt gaacaatcat
     2581 tgcttcttgg atcatttctt tgtttttcgg atgacgcaag aatttcagta attttgactg
     2641 gatttctttt tcgattagtt gtaattgttt gcgtacttta cgcaaattac gtgacgcgtc
     2701 gtcactgact ttattatttt ttattttctg gtcaatcaac tcttctacat ttatcaaatc
     2761 aggaagatgt ttgctataag caaacaacaa gggtgtctgg tattgattct tatcaaaaaa
     2821 ctttgtgatc atccgactgc ttctaagaaa atccgcatat tcgatcaaat cggttggctg
     2881 cagaatcaac ccttttttga cttgttctgt cagtgcatcg atcctcggca accccataaa
     2941 tggaacatgc tgactgcttt ctaagatcaa gcgtgcttct cttgtttctt cttgacgatc
     3001 caagacagtt tgtaggtttg tcgagaccgt catatcgctg attcgttttt tgctatagtt
     3061 tccaattgct cttgcttgga tttccttttt gatccgatca aattgtaatt gactaatcat
     3121 ttcgttgtcc atattctctc cttttcttga agagatacct gtcccaaaat aaataagggc
     3181 ctagagataa ataaaaggat agaacacagc ttaaaaaagt catgtcctat ccttattaga
     3241 atacaaagat attgttttcc tgacaggtac cgtttcataa gcacaccaaa taaactttgt
     3301 ttattggcta ttcgacttac tgattagata ccagctacac tcacaaaaca taccccattc
     3361 ttctaggtct tatttatttt ccacttaact atagcaagac acattttggt tgtcaaactt
     3421 tagtatgttt tatagattta caccattaat gaatagacac ctaccgcgat aattgctccg
     3481 atcattggac ctgcgattgg tacccatgaa tatgcccaat ctgaagttcc tttattagct
     3541 attggcaata ccgcatgtgc taatcgagga ccaaagtccc gagcagggtt gattgcatat
     3601 cctgttggcc ctcctaatga aagaccgatg gctaagatca atccacctac agcgaatacg
     3661 ttcgttccag gtgcaaattc gttttgtcca aaagctagca acccaagaac taatacaaat
     3721 gtaccaatta attctgtgat cacatttgct ggataattcc gtatagcagg acctgttgca
     3781 aacgttccta aaatcgcgct ttcatctttc gttgcattcc aatgtggcaa ataagaaagc
     3841 caaacaagaa tggcccctgc aaaagctcct aaaacttgcg caatgatgaa aggaacgaca
     3901 agattccaac taattgcccc tgtcatcgcc atagctagag agactgcagg attcaaatga
     3961 gctgggccca tgaatccaga aatatagaca gccattgtta cagcgagtcc ccaaccaaat
     4021 gcgatgacaa tccagccaga agcctgtgct ttacttttgt ttaaattcac agccgcacac
     4081 acaccatctc caagcaaaac taggatcagt gtcccaataa actctcccat gatttgagtc
     4141 atatcactgt tcatttattt ctcccccttt agttcccgca gatctgattc tgcaatcaat
     4201 gcttctaaac gtttagtctg ttgcgccttc tcctcttctg tccattcaaa atatccagca
     4261 attgcatcga tcacaggttg cttgatctca tcaagctgat ctcgttcaaa caacagatga
     4321 tttgtacgac gaatgagata atcgcctggg gccaaaacca tttcttcttc taaaccataa
     4381 cgtaaccgag ctgactcagc gagactcaaa ccaggataag gtgccatttc ttttgctaat
     4441 tcaaagatac gtcgggcatt agtcccgtaa aaatcagcga tataagtagc atcttcttcc
     4501 tctaaaccgg ctgctactcc ttcttttgct aattctgtca ctgtttcttc taatttagtt
     4561 ggatcgaagt ttccacctga aatctgatat tttttagagt cgatttcttt cgtctctatt
     4621 ccgtattctt cttttaataa ttggcgtatc aatcgcaaag ctccttctgc catcttacgg
     4681 taatcggtga ttttcccacc agataaagtc agcaaaccat ctggttctct ttctaaagaa
     4741 ctacctctgg aaatcgtaga aggtgctttt tcatcacggc tgttttccaa atggttcaac
     4801 acgtcttcta cttcagcacg agaaactttg ttttccttat attcacttac tgtatcgaca
     4861 actttattga agctcttgtc tgaaatcgat ccattatctc caccattgta atcagaacca
     4921 gaattaccaa tcaacagtgg acgaagtcct gcccagcttg cttcgatatc attcaatgtg
     4981 atatttgctt ctggatagcg atgattgatc acatccaata gataatctac atcttcttgt
     5041 gtgactttcg gatccgtaaa atctccttgg taatccgtat ctgtcgtacc aaagtaagtc
     5101 ttgttttctc ttggaatagc aaaaaccatc cgcccatctt gctttcctgt atcgaagtat
     5161 gtgggttgcg gtacaggcag ttttttcgca tctacaacta aatggatccc tttagttgga
     5221 cgcattttag gagagactgg acgcgtaaaa ttcaagtttc gtactttatc tacccaagga
     5281 ccactcgtat tgatcactaa ttttgctttg atttcgatca cttcatctgt cagcaggtca
     5341 cgagctttga cgccaacgat ttgatcgcca tcatataaaa agccaaccgc tttcatttta
     5401 cttactagat aagccccatc ttcggcagcc tttttgatat tatcaatcac taaacgggca
     5461 tcattgttgc ggaaatctag atagacaccc gcgcctttta gcccttcttt tttcaaaaac
     5521 ggttctcttt ccaatacttc ttctggtgtc agcgtataat tctcatattt ggttcctgtc
     5581 acatttgcca gtttgtcata aaggtccatt gctactttga ccgagaacat attgaaggtt
     5641 gttgctcctt cgccctcata gattggcaaa agcattggat ctggttttgg gatgtgtggg
     5701 gcaatacttt gtacgactgc acgttcacca actgtgtcag ctactacttc cacatcaaat
     5761 gtcttcagat aacgaatacc gccatgaaca agtttggtcg agcgagagga agtcccttct
     5821 gcaaaatcct gcatttcgat caatcctgtt ttgattcctg atgctgctga ctggactgcc
     5881 acaccggcac cagtgattcc gccaccgatg atcaacagat ccaattcttc ttgctgcatt
     5941 ttttgaatgc tatcttgtct tgtcttgttt gaaaacatat ggatttcctc cttattcaaa
     6001 tgcttgggta gcttttactg cttttttcca accgttgtaa agtttttctc ggcgctcttc
     6061 ctccatttga acttcaaata gcttgccagc ttcataaaat tcgcggatct cgtctgtatc
     6121 tttccaataa ccgacagcta atcctgctaa aaatgctgcc cctagcgccg ttgtttccaa
     6181 gttttctgct cgttggatcg gtacattcaa gatatctgtt tggaattgca tcagatattc
     6241 attgtttgct gcaccgccat ctactttcaa tacaggaatc ttgattccag tgtcatcttg
     6301 catcgtatcg atgatatctc tcacttgata agcaatcgac tgtaaagtag ctttaatgat
     6361 atcttctttt gtcgtaccac gtgtcaagcc aaacatagcc cctcgtgctg cctgatccca
     6421 ataaggagca cctagcccaa cgaatgcagg aacaacataa acctcatctt cgcttgtaga
     6481 gcgtttcgct gcgtcttctg aatcgctagc tttttgcaac atttgaagtc catctctcag
     6541 ccattgaatc gaagaaccag ccacaaaaat acttccttct aaagcatagt aaacctttcc
     6601 atttatgccg taaccaattg tcgtcaataa attatttttg gataattgcg gtttttcacc
     6661 ggtattcatg acaataaatg aacctgtacc ataagtattc ttgaccatgc ctggttcgaa
     6721 tgccatttgt ccgaataatg cggcttgctg gtctcctgcc atccctgaaa ttggtgcttc
     6781 gcttccataa aaatgataac cttgcgtata accataaact tctgagttag aagtgacttt
     6841 tggtaacatc actcgaggaa tatttagtaa atctaaaatc tcctgatccc aatctagctg
     6901 atggatattg aacaacatcg tccgactggc attcgagtaa tcagtaacat gtgcttgtcc
     6961 atcggtcagt ttccatacta accaagaatc gatcgttcca aacatcaatt cgcctttttc
     7021 agctcgttct tgcgcgcctt ctacatgatc aagaatccag cgtatcttcg ttgctgagaa
     7081 ataggcatcg atcactaacc ctgttttttc gtggaaaaat tcttgatagc cttcattttt
     7141 taagttatct gcaattccag ctgactgtct ggattgccaa acaactgcat gatagattgg
     7201 acgtcctgta tctttttccc atattactgt tgtttcacgc tggttcgtta tgccaatccc
     7261 agcaatctga tttggtttga tccctgattc gatgaacgct ccggcaataa ctgactgtac
     7321 agaattccag atttcatttg catcatgttc cacccaactt tcttgcggga aatattgcgt
     7381 gaactctttt tgagaactgc cgatgtgtct tcctttcttg tcaaagataa ttgctcgtga
     7441 gctagttgta ccttggtcaa tcgccatgat atacgttgat tctgtcattg tgattccctc
     7501 cattatatgc aaacgctttc tttaactact cttatcatac tactgtttta aaaaaagaaa
     7561 ctgtcaaaat tttattcttt ttttcttttt ttgacagttt tttattatga agctattttt
     7621 taatcaattc ttttcttttt tcagcgtctc tattcttctt ttcaatcgaa taatgtcgta
     7681 aggtgacgca aattctgaca gataatattc acactgtttt ttgtttctct ggcaaaatgt
     7741 gatcaccaaa tcatattctt taccttcttc ataacttcca atctcaatac cgccgattcc
     7801 cctcagttct ctgatcagat attgttgaaa agcaatacga taaatcggca gactttctaa
     7861 atcgattcca atcactactg gttttgcaat ataaaaatcg atcatcaaaa gaacattggc
     7921 atactggatc atcaggtaac taagctcact atcttcagct cttttcctcg gcaattgctg
     7981 caccgctgtt tcctgtaatt tcgccagtaa ctgtgttaag gaatgcccga gcattttctg
     8041 ctgctgttcc aaaagccgat cacgctcata gacttcgatc tttccaacaa agaaaaacca
     8101 ttcattgttg atctgggaaa tttggtagcc tactgccttt tcttctttga ttttcaaacg
     8161 acgaggacga taatgccata acatcgtttc ccgaatgtaa gtatctaaaa cagcggttgg
     8221 taactttttg ctccgtgtca aatcatactg ataaaatatt tcttcgtcta ctatcgaaaa
     8281 agaaacaaaa aagctgtaaa acatcattgt ttcgtataca ttcagttctg ctgctgttcg
     8341 actaagatgc atcacgatga ttggatcgat tgcttgataa agccggtccg actgatagag
     8401 taatttcttt tctttcaaag ttgcgtacgt acttttttca ttcaatagtc tttttttcgt
     8461 aatcccgagc cagcaagcaa tctgtgcttc tgctgatgcc gtaaacgttg tttctaatac
     8521 acggtccaat cctagaatga acggtctttt ttctggtgta ttttgcaaaa aaagcggtcg
     8581 ctgatcttcc gggatatatt ggaatagttc ataataaaaa tatcgtatct gtagctcttc
     8641 tccgtataat tgaccgtttt tgatttgtaa tccaaattcc tctaagagct ggttcaattc
     8701 tttgatcttt cgaaaaacag aagactcact gatattaaac gctgtcgcta atcgaacgat
     8761 ggatacttgc tgatgttcta ataaatagac gagcatctta aacttcaagc tttcctgcaa
     8821 taaaaacaat agaatttttt ccaaacttac atcttcgtcc aacacaagca gcgcctgata
     8881 ctcatttcgc tgtacttcta cttttttttc catcattcgg ccaatcaatt caatatcttc
     8941 caaatattga tcaaacgagg cttttgacag ttggacatgc tctctcaaac cattgataga
     9001 ggctgttccc ccttgcagaa caaggtttcg caaaatcagg atctgtaact gctcttttct
     9061 ttctaataat gcttcaatct tcataggcag tattcgccac ctttgaccac accatatctg
     9121 gcacacggta ttctggctgg aaaaatagct catgttcttc taactgttca aacgtccatc
     9181 cttcttctac tgcaattgtc agttggtcaa gaaggtggaa catctctttt ttgctttgga
     9241 gctgtgctcc aagaatcttt ttttcagaat caaatagaaa tttgagttta actttgcttt
     9301 gatttattgc tgtaagcttg tagtctacgt atcctgtttc aattttttgg ggataaaaaa
     9361 tgctctcttc ttctgtcaaa cctacgcttc ctaagaaata gccaaaccac tgattgccga
     9421 ctggacgata ggttccctga tcctttgtct gtgagccact aattgtcttg ctgacatttc
     9481 gtgctgtacg gacagcgtta tttactagag aaacatacat tttttcattt gttggacgaa
     9541 aagttacttg gatcgcatcg ccaatcgcat aaacgtcttt ttgagacgtt tgcaagtagt
     9601 catctaccca gatcgttcca tcatcattca atgttaattg ttcttcccac atctgattgt
     9661 cggggcgaga atgattagcc agcaaaacaa gatcatagtt ctcctttctt ttttccgtct
     9721 ctacaatagc ggtttcattt tcggtcaaag acacaacttg ttcttctaaa aaaagtgaaa
     9781 gcccttgatt tttcatttcc tttactaacg gttctgtcat ttcaggatct agataacgaa
     9841 aaagaattga ctttcggctt tcgtaaagat ggatttgttt ttttcttgat gccaaccctt
     9901 cagctatttc gattcctgct tgtcctgcac caataattgc aattttcttt gcattcatga
     9961 tcttttgctg aagagccgtt acgttttgta tctttttaac tgggtgaata agcgttgatt
    10021 ctgcagaaat atttcgaaac ttttgatttg aaccagtcgc tagaatcaaa cgatcaaaat
    10081 ccaatttttt tcctcctgct aaaacgactt ttttttcttt caatcctatt actgtctttt
    10141 ctgtgtaaac atggattcga tagcggtcag tcagttcttc tttcgtgatc caagaaagtt
    10201 gtttttcact ggtaaccttt ccttttaata tcaaattgaa cccgctggga atccatccta
    10261 acttcgattg cttttcaatc aaatatattt cacttgttgg catcaattgg cgaagataaa
    10321 tagccgcatg aatccctgca aacgacccgc cgacaattac aatgttcatc tgcattcctc
    10381 tttcttataa ggtgttctac tgctaacttc tcaatcagaa aacacagtat tcataactcg
    10441 ctttcaacct tatgatacat agcgatgcag cagaatcaac cctctccatc ataccgtgtt
    10501 ctaggtctgc ttctcaataa tgtactagtg gacctaattg aagagtatca tttctgaaga
    10561 gatgtttcaa aaaatgtact cttataaaag aaaaaaactc gccttttacg tacctctgtc
    10621 caaataggaa acagaaaaga acgaaaagac gagttttagc gtgtgtaatg taatagttag
    10681 aatagacaca tcatttattt ttaattcttt aatgcttcaa caactgcttc gcctaattga
    10741 tgagcagatt gaggattttg ccctgtaaga agccgcccat caatttgaac cacttctgaa
    10801 aaagctgctc cagactgata gtttgctcct ttttctttca aagcatcttc tgctaagaat
    10861 ggtacttcat tcgttgtacc atttgcttcc tcttcttcat tactgaaccc agtcactgta
    10921 cgtccagcta aaatagattt tccagactca tctgtaactg gcaacaatcc tacaacaccg
    10981 tgacaaacag ctgagaccag cccttcattt cgatagattt gtgaagcgac tttcccaaga
    11041 tctattgctt ctggaaaatc ccacattgtt ccgtggccac cagcataata aaccgctgca
    11101 taatcatttg catccacatc atcaggacgt aaggtatttc ccaaggcccg atttctgaaa
    11161 tcttcatctt cataataggc ccaatcgatt tcatccatat tcaaaacact ttgcggttct
    11221 aaaggtacat atccgccttt aggactgaca aaatctgcat cgatcccatt ttttactaat
    11281 acatcataaa aatgagtcag ttcacttaac cacaaacctg taggacgctc gatcttttcg
    11341 tacttttcta catttgttaa tacgatcaat actttcattt tcccatcctc tttctataaa
    11401 aattgcgtta ttgataacgt gctttcaaga aattatataa tgggcttggc aaataaagct
    11461 ctttatatgc ttcttcaaaa ggtttcgtta caaattcatc ctcacggttt tccaataatt
    11521 tctctgccca gtcaggatcg acgatcattt gctgtccaat tgctgcaagt tctgcatatt
    11581 tcaatacctg ctctacatct tcgcgattgc ggatcccacc gacaccaact aaagggatct
    11641 ttccttgaat cgtgttatgg accacttcaa gaatcgattt atcgttgtat ttatcagaac
    11701 gagctattcg atcatacata tttaatgaaa catgcagata atctagtttt gactcacgta
    11761 atcgttcaag aagccagatc gtgtcttcaa aacggatacc tggtgtttca tattcttctg
    11821 gtgagaaacg ataccctacg atgaatggtt ttttagcata ttgttcaacc gctgagaaaa
    11881 ccgttgttaa taattcttca ataaaacgat aacgattttc caagctaccg ccccattgat
    11941 cctctcgtct attcgaatgc ggagagaaaa attgttggat caaatacgta tttgctccat
    12001 gcaactcgat tccatcaaat cctgattcaa tcgctctacg tgtggctttg ccaaatgctt
    12061 ggattgtttc aacgatttct tcttcactca ttgctcgtgg tgtttccgca tcaggacgtt
    12121 ttgctgcaac tgcacttgca gatacaggct gttctccaga taaagttgct cgacttgtca
    12181 ttcgtccacc gtggaaaatc tgaataatcg cttttgcgcc ttctccttgg attgctttcg
    12241 ctagctcact taatcgagga atcatttcat catgagcgat gctgagttca cctggccagc
    12301 cttttccgat atcttggaca tttgcagcac cagtaataac agcaccaaga cctttagaac
    12361 gttgtttata ataagtgatc tcatcttcgg tgatcactcc attgaaaaag ctgagttgtg
    12421 tcgtcatagg cgacagcatc aatcggttct tcaattgaac tcctgaaggt aaagtaattg
    12481 attgatcaaa ttgtgacatt gtcattcctc ccataattcg ctctgattaa cctatcgaga
    12541 atcatctatc attaaaccac tcgattagtt gtacatgcaa ctaatttgct taaaaaattt
    12601 tatttttttt gtaacttatc tgttgcatca ttcattgtct gggctaattc tttacttgct
    12661 tcatttccca aaactgcttt gtatgactga tgaagttctt cccagctacc atcgacttcc
    12721 ttagcaaatt ggcgtccttg ttctgtaaga taaactaatg acaagcgacc ttcgctaact
    12781 gttttgacgt atcctttata ttctaatttt tcaacaaaac gagcaatcgt tgatggggcg
    12841 gaatagagca tttgtgcaat atctttttgt ggtattccat ctttttcttt taatatcaat
    12901 aaaataaaac cataagttgg tgccagacct gtccttctaa acgcttcatc agctaattta
    12961 ttcaacatgc gatctagctt tttcacgcta aagaaaagac attcatctaa cgctttttca
    13021 attggctttt ccatttcatc ccctccattg gcgtacatat catagtatga tttttatttt
    13081 ttgtcaaaaa acacggaata atgtccgcct cctctgtcta gatttgaaaa aaagaagtag
    13141 ccatcttcaa agaatttcgg cttgttttcg acaaaactcc ttctggaaca ccgtgaatca
    13201 gaggttgtga aaaagtcgtt tagttttgag tatgaagaaa aattttgtga aaatggcttt
    13261 ccacattttt gcataaattt ggcttaatac cgaaaaactg gcttttgaac accgtttata
    13321 tcagaggttg tgaaaaagcc gtcctgcatc aagtaataag aacagccaaa caaaaatagt
    13381 tcctcatatt ttgtcgtttt ttgagctaat gccgatggtc agctgattgt acaccgttta
    13441 ttgagaggtc gtgagcctgc cgttgaactc cgagtcacaa ggagcaattt caaaaaacag
    13501 cttctcatgt tttatgacta tggtgacttg tgatcgagga gttggcagga gaatactgtt
    13561 tattcgtaaa gaaggggctg tgacaagact tttgtcacag tcccttattt ccttttatta
    13621 taggatttcg ccatttgatt ggatgacttg tctgtaccaa tcaaatgatt ttttctttaa
    13681 ccgtttcttt gtgccattcc cttcgtcatc ttgatcaaca tagatatatc cataacgttt
    13741 tgacatttgt gaagtagaag cgctgacgat atcaatgcag ccccacgacg tataaccaaa
    13801 taaatctact ccatctttga tagcttcctt catctgctca atatgctgtt tcaaatagtc
    13861 aatgcggtaa tcgtcttgaa cggttccatc ttcttctact ttgtctatag ctccgagtcc
    13921 gttttcagct atgaacagtg gtttttgata cctatcatat aattgattca aagcaattct
    13981 caaaccctta ggatcaatct gccagcccca ttcacttgat ggaagatacg gatttttaat
    14041 tcctccagtt attaagtttc cttggacccc ttcccaattt tctggatgag cactgactgc
    14101 actagacatg tagtagctaa aagaaacaaa atcaacaggg taggctgcta agatttcttc
    14161 atcacctact tctattttta agttgacacc tttttcttca aacattcgtt gcgtatacgc
    14221 tggataatat cctcttgctt gaacatcact aaagaacaaa gaagttcttt gctgctcaat
    14281 tgttttttga atatcatccg gatcacaaga atacggaaca agaagctgat agctgatcat
    14341 acaaccgact tgtgaacctt tgataatttc atgacaagct tttgtagcta aagcacttgc
    14401 tataaactga tggtgtgcac cttggtattt cgcttgttcc aaatggggat ggttttcttc
    14461 aattactcct acgctaacaa aagggtgatg ttttacacag ttgatctcat tgaaagtcat
    14521 ccaatatttt actttatttt tatagcgttt gaatactgtt tcagcgaaac gaacgtaaaa
    14581 ctgaataagc tttcgattag gccatccacc atattctgta accaaatata acggcatttc
    14641 ataatgggaa agagaaacga ctggctcaat cccatatttt agacattcgt caaacacttg
    14701 atcataaaaa aataatcccg cttcattggg tgtttcttca tcaccatgag gaaaaattct
    14761 tgtccatgcg atagacatcc gatagcattt aaaacccatt tcagcaaata aacggatgtc
    14821 ttctttaaag tgatgataga aatcatttcc gtgtctttta ggataataat acttgctgtt
    14881 gggatcctgc gccgctttta cttgctgatg cgtcatcata tgccattgat ctgtccattt
    14941 ttctttaggt aaatcgctgt cgaatgtata cacatcagac acagataatc ctttttcatc
    15001 tacatcccat gcaccttcac attgattggc cgctgtcgct ccgccccata aaaatccttc
    15061 tggaaatttt ttcatatcga tccccctaaa cttgctagtt ttcttgtttg attatttttc
    15121 ttatgactag taatgatatt tttgaatcta ctactatttg taacaataac cattgtggtc
    15181 ggatcatatc cctctttgat taattcttgc aaatctactt ccatcaataa atcgccttta
    15241 cgtacttttt ttccatcaga tactgcactt tgaaagtatt tgccatttaa attcactgta
    15301 tcaataccga tatggatcag tatctctgct ccttcttcac tgactacccc aattgcatgt
    15361 ttcgttggaa aaactgttga aacttccccg tttacaggac tgtagatttt tccgtccgtt
    15421 ggaatgatcg caaacccttc tcctaaagat ccactagcaa atgttggatc gtttacttca
    15481 gataaatcaa ttatttcacc tgctgcagga gtacttaaca ctatatcctt tgaggattct
    15541 tcttcgattt catccttgta gccgaaaaga aaagttagca tacaggaact gatcatcgct
    15601 aagaaataac tagcaactag ccaaacaaat ccttcaccaa tataaatcgg gaaagacaaa
    15661 atacttcttg tagcaactgc caaagcaccg gctcctgcgg caccagctac tccaccaaat
    15721 atagctccac atatacaagc aatgtaaaat ggtcgtttat atttaagagt cacaccataa
    15781 acagcaggtt cagtgattcc aaataatgct gtgataaatg cagataaaga taaacttttt
    15841 actttcttat ttttcgtttt caaaaatacc ccaagtactg ctcctgcttg cgcagctacg
    15901 gcagtcatac atgctggacc tagcgtatca cgaccgtaga aagaaagatt gttggtcatt
    15961 actggtacaa gtccccaatg taacccaaaa atcactaaca cttgccaaaa tccgccgata
    16021 acagcaccag ataaaacagg gctaaagtta taaacagcta aatatccagc tgcaatcagc
    16081 tgacttgcat aatctgcaat tggacctaca actaataaag taattgggac catcacgatg
    16141 atacaaatga atggtgtcaa taaatttcga atactttcgt gcaagctttt gcgaatctta
    16201 ggctcaatat aagaaagaac ccatacagct aaaattgctg gaataaccgt acttgtatac
    16261 cttgctagaa tcacgggcat ccctagaaat cgcaaggtta tgctatcact atatgcactg
    16321 acaatcgtag gataaacgag agctgctgca ataaccatag cgatgaaacg atctgtattg
    16381 aacttttttg cagcagtata tgccaaaaat ataggcaaga aatagaaaaa tgcatcagct
    16441 gctgcattca aaatgatata agttcctgat tcagtagtta ataaattcaa actcgtacat
    16501 aaggcgagta ctcctttgat caaaccactt cctgccaaga cgcctaaagt cggggcaaaa
    16561 acagcggcga tcatgtctat aaaagagttt aaaatgcctt ttttctcagt tcctctcttt
    16621 ccactagatg catcatcttc tagtttcatt tgtttggata atacgtcata cacttcattt
    16681 actgcatttc ctactacgac ttgaaacatt cctccagctt cgactacagt gatcacacct
    16741 tctagttgct ctaccttctc cttatctgcc ttcttattat cttttagggt aaagcgtaac
    16801 cttgttgcac aatggtaaac attgctgaca ttttcttcgc ctccgacaaa tcggacaata
    16861 tctttcgcta attgtgaata gtccattttt acttccccct tgttaattaa tgccgtagca
    16921 atccttctta caagataagt atagttctta gaaaacggtt tctccattca attagtaccc
    16981 cttataatag cgaacgaaac tgtacaatag tgtgtatggt ttacttagcc gagcaaatca
    17041 ctcactgctt gaaagaaaac aagtatatcg tttctcttat agtcaaagga gacaaaaaaa
    17101 acacgcttga tcttcctttg ctgatgtata agtggtatct caagctgata ctctgtgatt
    17161 tttgtttcta cttggctttt gataagacag cacaacaaag tctcggcatt taacggaagc
    17221 ataactaggt cagaactagg agagattagt tgctgaatgg aatctatctt tttatggatt
    17281 tttatttgat tttcttcttt gtagtaatga tcaagcagtt ttgttttctt tttttcttca
    17341 actatggtca gcttttcacg caaaatctga taaatctccc aagattcaaa ctcatctaga
    17401 acactaggta gataaatcaa atcatcccac tcaaaaataa gctcttgctc agtagtcaaa
    17461 attgcatcat attccgttat tttgtttgat tgatttttga aaagatacac tggttctatc
    17521 cggcattctt tgatccacat gttcatactt tgttgaatct gtttttttag tgtattcaaa
    17581 atactaattg gctggttact aacaatcagt atctcccctt gttttcgaaa aggattcaac
    17641 gcttcggcaa gatacaaaac aaaataagcg ctttcagcta aattgggacg taattgaaaa
    17701 tgtattggga agaaacgccg aattaaataa atttctaaag gataacggga gatgatcttt
    17761 tgtacaaaat gattcatggt attatgtccg gcatccatcc tcgttttcat ttgtttgata
    17821 tgggttagca aaaattctgt ttcatggaac aataacgaaa caggaagatc taaaaaacga
    17881 ataatcgttt gctcaaatgc tcttagattc ttctctattt ctttgttttt ttcatcatta
    17941 atcatcaaat aatttaactc taatagtcga ttttcaattg cttgtttttc ttgattggaa
    18001 aaagaatagg gtagttctga tgataacttt tttgtgaggg tctctaccat cggaataaat
    18061 aatgtatacc tgtttgtttt ttctaaaagt tcttctcttc gaagaatggt caaaacgaga
    18121 taccgagaaa atcgtatgaa gtcctctcct gaaacaatat aattatatga tataagaacc
    18181 tcttttaatg tcttttggac agtcggcatg atattggaaa aaattgtact gatttgttga
    18241 ggcaaccttg cgtgttggat caccgtttct gttccaacaa gagataagaa aattcttttt
    18301 gaattttctt cagcaatgat ttttagtcct tttgtcgacg aaacttccag ttctaattta
    18361 ggattgcgct cgatccaacg caaaatcgtt tttatttgta atgatacggc tgtttttgaa
    18421 agaaagaaaa tgtctgctat cttttgcata ctgacatacc ctttttgaaa caataagtac
    18481 gtcacaatag ttgaagctcg gaaatccatg ctggaataga ttttatgttt cgtcacctct
    18541 tgtatgattt gctgtttagt ttcagattca attgattcta aaaaataacc tgaacgttta
    18601 tttgactgga taatgatttt cttctcgtag atagcaagca tatttttgat ttcagtttgg
    18661 atcgttttct tgttggtacc taaagcttca gctaattctt cgcttgttat ccaatgattc
    18721 ttatttctta atactacaag aatttggagt tgtcgtttat ttaagtccat ttgcttacac
    18781 ctcctctttg ttataaacat acaggaaaaa cctctcaaat gacaatagtc acttgcagag
    18841 gtttaaaaat catttgaaga aagatctata ttcaaggttt ttaccattag actggataat
    18901 ttgtttataa tattcaaaac tatctttttt caatctccta aagcttccgc tcccatcatt
    18961 ttgtctatcc acataaataa agccatatcg cttcctcatt tcccctgttc ctgccgatac
    19021 gatatcgatt ggtccccacc ataagtaacc aatgatttgg caaccatctt ttattgcctc
    19081 agcaatttcc tgaagatgta tttttagata ctcacaacgt tttggatcgt ggattcgatt
    19141 caattcatct ggtgcttcat ctagtccaat gccattttca actatgaata atggtaattc
    19201 ataacggtct gtcaattcat tcatgacata acgaaaccct agaggatcaa ttggccaact
    19261 ccaaggttcg ggagattttt ccttcaaaaa gggattatcg aaaccatttg ctccgccagt
    19321 atctacgcgc atttctgctt cttttttata gacagcgcta cgataataac taaatgcaat
    19381 ataatctgcc gtgttagctt gcaagcactg ttcgtctcca tctaacatta ctggtgcaca
    19441 gtcttcttcc ttccatgtcc tgtaaatata agctggataa tgccctcggc acatgacatc
    19501 ggagaaaaac catgaattgc gtttaaattg cagtgaacca aatacattct ctggatcaca
    19561 gtcatatgca taagtcgcta atgcagaaaa gctacacata atacccatct tagcatttgg
    19621 atcgattttc tttaataatt gagtagcacg ggcgttcgct agaaacatat gatgacaggc
    19681 ttgataaatc actttttttg ttagtgtagc aatgggttca agtttatctc tagcagggaa
    19741 tgtaaccaat ccagcagctg cataaggcat acgaaaagca ttattgatct cattaaaggt
    19801 catccaatat ttcactttcc cttggtagcg ttcaaagacg gtctgcgcat aaaattcaaa
    19861 aaagtcgatt aattttcgat tttgccaccc cccatactct cctaccaatg ctaagggggt
    19921 ttcataatga ctaagagtaa ttactggctc gatatcatat cgatttaatg tattgattag
    19981 tctgtcataa aatacgagac cagcctcatt tggcttctgt tcgtctcctc ttggaaatat
    20041 tcgtgcccat gagatgcttg ttcgaaaagc ttttaaacct aactccttca gtaaacgaat
    20101 atcctcttca aatcgatgat aaaaatcaat cgcttcatga gaaagataat ggagtgattc
    20161 gtctaaagca atctcccaaa tctttttttc ttcattccac tgaatactag gatgctggtt
    20221 catgatcccg ataagcgtat ctgtcacatt aggtaatttg ccgtcttcca accatgcacc
    20281 ttcacattga ttagcagcta ctgctccgcc ccataaaaaa ttttctggaa atatttcctg
    20341 atccataact atctctcctc gctatcaaca atttcttact tgaagtatag ctcgattcat
    20401 caaaacaaga tattctaact atttcggcag aagagaagaa aaaaattgaa tgctactctt
    20461 catttttaat atttatatat aacaaataca taagacgatc cccgaaagaa aacactcgtt
    20521 aaccatctaa gcaaacgatt tattttactt ccaacacaaa tgcttcataa ggtcgtaaat
    20581 caactttgcc gtctggtaac tgtttcagag gataatttga cacaataacc cttaaagctt
    20641 cacgtggaac agtgaacgtc tgcttttcac tggacatatt acaaataacg agccattgct
    20701 ggtcttcata tatgcgagaa taggcaaaga catgctcagg cgtcttttcc actaattcat
    20761 agtctcccca gacgatgatg tcatgttttc tacgcaaatc gatcaatttc ttgtacatat
    20821 aaaaaacaga atcttgatct gctaacgctt cttctgcatt gatttcttta taatttggat
    20881 tgactcgaag ccatggaatt ccagtagtaa acccagcatg ttcacgggcg ttccattgca
    20941 taggcgtcct agcattgtct cttcctttcg cattgattga ttccaagatt tcctcttttg
    21001 taaatccaga agaaatcctt tcgttataca tattgattgt ctcaatatct tttgcttccg
    21061 cgatatcatc gattgggcaa ttcgtcatgc ctaactcttc tccttgatag atataagggg
    21121 tgcctttcat catatggagc aagatagcaa acatcttggc actttctttt cgatacacat
    21181 cgtcatttcc ccaacgagaa acaattcgcg gaagatcgtg attattccaa aataaactat
    21241 tccatccttc atcacctaaa gatgtttgcc atttggataa tacttttttc agttcagaga
    21301 tactcaaggg tttcaaatcc catttgtctt tgccttcttg ctgatctaat cctatgtgtt
    21361 cgaattggaa gaccatggat aattcatttc ttttaggatt ggaataaagt ttagcgattt
    21421 cggtagttgc tccccatgtt tcgccaacag tcatcagatt tttgtcacca aaagttgctt
    21481 gattcatttc ctgaagatac tcatgcagtt taggtccatt acttgtgatt tctttatccg
    21541 gttctttgcc aatcatatcg atcacgtcca tacgaaagcc cccaatacct ttatcgatcc
    21601 agaaattcat catttgataa atctcttggc gaacctgttt atttttccaa ttcaaatcag
    21661 gttgacgctt gctatacaag tgcagaaaat attgccccgt tttttcatcc aactgccaag
    21721 cagaaccact gaatattgag cgcaatctat taggctcttt tccattgacc ggatcacgcc
    21781 aaatataata gtcccgataa ggattttctt tatttttacg ggcctcaatg aaccacggat
    21841 gttcatcaga agtatgattc accaccagat ccatgatgat acggatattt cttttctttg
    21901 cttcttcaat cagttcttcc atttcttcca tcgtaccgaa ttctgccatg attgcttgat
    21961 aattactgat atcataccca ttgtcatcat tcggagactc gtatactgga gaaagccaaa
    22021 ttgcatcaat acctaaatac gccagataat ccaacctgga aataatcccc cgcaaatctc
    22081 caataccatc ttgatttgaa tcttgaaaac ttcttggata gatttgataa acaactgaat
    22141 ggtgccacca tttttgttcc atgattttcc tcctataaat ggatgtttct attttttgct
    22201 taatcattta ttttagtctc tctcactaaa aattgtaacg ctttcaaata gctattgcac
    22261 cttgttatcg gtaacagaat aaaacaaaaa aacagctgag gaaattccct caactgtttt
    22321 ttgaaaggat tgatttatgc agggaagaat gctgatttca caacttcttt ataaacggtg
    22381 ttcactcagc tgtcccttgg tattagctca aaaaacggca aaatatgagg aactattttt
    22441 tcgttttttt ctctaatact caggccagaa cactgatttc acaacctctt tataaacggt
    22501 gttcactcag ctgtccctcg gtattagctc aaaaaacggc aaaatataag aaactatttt
    22561 ttcgtttttt tctctaatac tcaggccaga acgctgattt cacaacctct ttatttcaca
    22621 ttcaatatcc acatgaatcc cacaaataca aagaacagac catacatgat tggagatact
    22681 tcttttcctc ttttagctgc taacatggta attggataga agataaaacc taatgcaatc
    22741 ccatctgaaa tactataagt caaaggcatg ccgatcaaga tcaagaatga agggatcgca
    22801 atctctaagt ttccccattc gatttgtcgc aaagattgtg ccatcaatac tcctaccata
    22861 atcaatgctg gtgcagttac ttgtgaagta acaactgata gtaatggaga aaagaacaag
    22921 cctaacaaga agaaaatccc tgtagtaata gctgtgatac ctgaacgtcc tccaacggcg
    22981 ataccagctg atgattctac aaatgcacca acaggagaag taccaaataa ggaacccgca
    23041 agcatagctg ttgaatcaga agctaaagct ttccctacac gaggcatctt gttgtctttc
    23101 atgaaacctg cttggtttgc taatccaact aaagttcctg ctgtatcaaa gaaagtcaca
    23161 agcaagaatg tcagcactac gacccacatt tgaagagaat tgatgtcccc tacatgtttt
    23221 aatgcaacta aaaatgttgg tttcaaactt ggtgctgcgg caacaatttc agaaggcatt
    23281 ggaatcaaac ctgtgaccag acctaaaatc gttgtagctg ccatcccgat gaagatgcca
    23341 cctggtacac gccgaacaag caagatagca gttacgacta atccaaaaat cgtcaaccaa
    23401 gtagaaccaa cgttgagtga tcctaatcct actaatgtag attcattcga aacgatgatc
    23461 ccaccttcgc tcagaccaag gaaagcgata aatagaccga taccgccaga aatcgcatat
    23521 ttcaaatctg ctgggatcgc atcaatgatc atttcccgca gcttgaagat tgtgatcaaa
    23581 ataaaaatca aagaagcaac gaatacgccg gctaatgctg tttcccatgg aatccccatt
    23641 cctaaacaaa cagaataagc aaaaaaagca ttgatcccaa gagcaggtgc cgttccaatt
    23701 ggataacggg ctaatacccc cattaaaaca cagccgatcg cactggctaa tgcagtcgca
    23761 gtaaacactg ctccttcatc cattccagaa gcacctaata cagttggatt tacaaataag
    23821 atataagcca ttgagataaa tgttgtaaat cctgccagta cctcacgttt taccgtggtg
    23881 ttcaattttt ctatttcgaa gtaggaaagt attttttcct tcatcttcca tccccctaaa
    23941 tccgaacatt ataaataaag tccgttaaaa tttcgattta ttaataaaac aacgtgtttt
    24001 atcatatact aaaacaaaac ataagtaaac aaaaaattcg gattttaagc aaaatccgaa
    24061 cgttccatct gattaaatcc ctttatctca aagttcatga acattcactt tggattacaa
    24121 gaatttcttt attgtgttaa taaagtttct tcattctagt ctcaaaaatc aatagtgtga
    24181 tttgatttta ccatttcgtt tctgttcttc tttgatgaat tcttttgtat cgacaggatt
    24241 cagattattt agtccgccaa aacctgtatc gaccattttt ccccagcctt ttgtcttctt
    24301 ttttcctgat ttgagattct ttgctcgaag cttctcacta tgttttgctc gccgatactt
    24361 caaataacct tctaatcttc ccattagatc cactgttttt cctccttttt attcaatcag
    24421 acgactcagc ttttcctata acaaaataat tgccttcagg gtcagaaaaa ttgaaagtca
    24481 agatgccatt gttatctgtg atttctcctg gttcttccat gttctcgtag atagtttcaa
    24541 attcatccgt aaaaaatact aaagaaggcg gtggtcctaa tacctctggt gaaaacttct
    24601 gcacaaatac ttttgggaat acgcctaatt ctaattcttt tgagatgctg agtacaatac
    24661 tcttaaattc ttctggcaat tcgattgttt cagaaatttc tgcaccaatt ttttctacaa
    24721 agaatcggca aatcatctct acgtcattga cataaagcat cacacgtgtt ttttgtatca
    24781 tcatttgacc cttctttcta tttgattttc tttcctgtct ataaactgat tccattatat
    24841 cacgaaagcg caaacacctt gtgtttgata ctattcaaga aaaataaagc aatagtttct
    24901 aggattgctt tcctccacaa acttttttta caaaaaaaaa aacggcaatg aatgccgctc
    24961 ttcttatttc gattctttta taattctgct tctgattcat ctttggttag tgtgtgtcta
    25021 acactagtta cgccaaatgt ttgatcgatt ttttccttca acgcttcgat tgcctgtgcg
    25081 ccacgtgccg cttgttcagc attgatggat tcaatgacca gcaccgctgc ttgtgtttct
    25141 tccgtcagca ttgttctttg ggcttcacgc cagttgagac agcgacagat cgctccttgt
    25201 tcatcatagt agatgatttc ttccggaagt gccggagcat cggaatctgc acctaaaggc
    25261 aggaatgatt cgccaccttg ggcttttcct aaatgcagat ttcctgcgat cttttcgata
    25321 tcttctccgc cgcaaggtac gccaaaagac aaggaaatac tattatagat atctacaagc
    25381 ggattgattg ggaaaaattc ccgttcttga tttgcacgtt tcagtaaagc ttcaatagaa
    25441 gaacgtgcac cttttttagt cttgaatgtg gtaaatgcct gacgccactg agcaatgact
    25501 tcgttttggc tgaatgtttc ttctgtaagg taatttcttg cttcttttgc tgcctcattc
    25561 aataattgat gatacgtttc ttcactttct tttgttacgt ggttatcgat accatttacg
    25621 gataagattt cgatggttgc ttcaggaaat acttcccaga aattggtatc cactattact
    25681 tttttcatct gttcacacct cttgattaga ataattgctt gatgaataga tagtctgttt
    25741 ggggatcatc tcccatataa aaaacatgtt cgctgtattt gataaatcct tttttacgat
    25801 aaaaagcgag cgcaacatcg ttgtgttccc aaacaccgag ccaaatggct gttttttgct
    25861 tttcttttgc tttttcaata gctgtatcta gtaaaaatgt accgatacct tgatgtttaa
    25921 atttttttcg tacatagatt cgctcgattt ctaaaaaatg atcagccatt tcctcagatt
    25981 gactcgttcc tgtatttagt ttcaggtaac ccgcaggctc ttgatcgata ctcactagga
    26041 aaaaatcggt ttctttatct tttagctctt ctgtaagttt ttctagtgca taagcttgtg
    26101 tcaagtaatc agctaaatgc tccggtgaat tatcttttcc aaatgtatct gtaaatgttt
    26161 cgatactaat ttgttgtagt aagtctacat ctgaaggcgt catttttcga atttcgtatt
    26221 tattcacggc ttcttccttt caatagatcc ttttattgcc ttttttgaca aaattccaat
    26281 cttcgctaac attcttgcat actttattta aaagatattc caattgtttt gcttcttcgt
    26341 cagaaagacc atttaatgcg actgcattgg agtagttgtt ttctcgaatg atgaaaggat
    26401 agatttctgc tcctttttta gtcggataga gtttcttgat ttttttattt tctttatctt
    26461 ccaaacgttc gatcatccca ttggattcta atttcttgat tgctcgggca gtcgttgtac
    26521 gatctacttt gatcatttct gctagttttt cttggatgat tccaggattt tcgcaaatac
    26581 gtactaaata aagatactgt cctcttgtta aatcgacttc tttgaattcg atattgctaa
    26641 tagaatctag cgctcgggca atcattccga tgtctcgaag gatttcagcc atttcatccc
    26701 acctttattt tgtttgtaag actagtttac ctctttttgt tgcaaatgca actattttca
    26761 ataaaaaaat caagttcttt tttaataact agtaaagtat attcttttct tcttttacga
    26821 aaaagctcaa tcacaataga tctggttttt gttttctaaa ataagtaact gtttcttcgt
    26881 tgttcgcttt tctgatagct ttggcaactc ttctatacta aaaaatccca tgttggaagt
    26941 ttcattattc tcaataaact tggcctcttc accgtggatc gcacaagcaa aaacaagttt
    27001 ataatactga aaaagctgtg gaatatcttt ttgtttattc gtatcaaaaa ctgctcgcaa
    27061 ctgagtagtt tctactgtta atcctgtttc ttcatagact tctttacgca cattttcttc
    27121 aggagacaat cctatttcag caaatccacc aggaagtgac cattctttcg tccgtttatc
    27181 ttcgattagt aggaccttgc catctttttt gatataggca cgaacatcta cttttggtgt
    27241 tggatgtcct tctccaatcg ttgtgagcgc ttttatttct tcgaaagttt cttttcctat
    27301 ttcactgatc agttccaatg aaatcgtccg gatctcctca aaacgctctt tatcaaatac
    27361 atcttttcca taatatagac cagcttgaga aagtgcgagt agtcttttgt attgagccaa
    27421 cttcttgttc attctcttcc cttctttttt agcaaatgta tgactatgta atcaagtata
    27481 gaagactggt ttaattacgg caaataaata taatgaaatc tgcaataaat aaaagaatgt
    27541 taaaaaactc gagaacaaaa gacattcgac ttctctgggg ctgtgaataa atggcgttca
    27601 aaagcttgca tctgcggtaa taagccccac caaacgaaaa atatgacgag ctattcttgt
    27661 ctggctgttc ttattaattg aggcaggaca gctttttcac aacctcccca ctaaacggtg
    27721 ttctcctacc aactcctcgg tcacaagtca caatattcat caaacatgag aagctgtttt
    27781 tttgaaattg ctccttgtga ctcggagctg aacgacaggc tcacgacctc tcggtaaaca
    27841 gcgttcaaaa gccaattttt cgatattaag ccatttcttt gttacttatt gtcattgatt
    27901 tctttttcta atttatccaa tagtggagtg taatccactt gagtatagtc tgttacatca
    27961 aattcatata gtgttcctaa ctggaatttg tcatattttc ggtcggcgat gatctttttg
    28021 aatgcttctt tcgtaatatg attggtccct ttcgttcgat cttccaatac gtcaccatag
    28081 tgataatgat ccatgatata gctcaaatga cgatctgatt cacgatcacg tttgtaccga
    28141 cgttgacata acacttcaaa gtctgctgtt aggcgaatcg tgatcacctg atatccgtat
    28201 ctatctgcta attcttggag tcgtgctttt tgcttatcac taaaaggata ctcggacaca
    28261 atcactttct ttcctgcttc catatacaaa tcaagtgctt tgtaatagaa aggataaact
    28321 ttcgtccttt cttgttccgc ccgttcttcc aaattgttaa acccgatgga ttctgcatag
    28381 tattccttga tttcatcgag cgtgatggca tacatatctg gaaatcgttc ccgtagttta
    28441 ttcataagat atgtttttcc tgtaccagga ctaccagcca gaagaatgaa atactttttc
    28501 atctcattta tttcctaagt taattggact ttccaagttc ataaacagtc caatcttatg
    28561 cttgacaatt tcttttactg cttcatttgc tggaatcaca ttatggcgaa gagacttgtt
    28621 gatttctgtt tcactaaagg tttctttagc tactttcgtc cagttgacta aaagctccgt
    28681 tccaacattg aatttgcgga tcccgttatt gatcaattca ggataatctt cttctttcac
    28741 acctgttcct ccatggataa ctagggggac atcaactact gcatcgattt ctttcagcaa
    28801 gggaatgttt acttctgttt ttgatttgaa ttgtccatga ttcgtaccga tagcaatagc
    28861 taatgcatcc acgcctgtag cttctacaaa tttcactgcg tcttccggtc ttgtatatac
    28921 cttgtcattt tcagcgacat ggattccttc ttctgtaccg ccgattgttc caagttctcc
    28981 ttctacggaa acaccgtgcg catgggcata ttctactact gcttttgtct tcaggatatt
    29041 ttctttgaaa ggcagatggg acccatcata catgacagaa gtatatccgc tgtcgattgc
    29101 ttctttgata tcatcaaagt ctcgggcgtg gtctaagtgc aagactgcat caaccatttc
    29161 ttcttctgca atagctgtca ttacattgac taacacatgc atgccgatat atttagcggt
    29221 atcgacactt gtttgaataa tgatcggcga acccatttct tttgctgcgc ctagcatagc
    29281 aggaagcatt tctaggttat gcgtattaaa agctccaact gtaaaattca actcttctgc
    29341 tgtttttgtt acttctttca atgttgtata catcttttat tgccacctta tctttttatt
    29401 tgtttccttt gatcgttact tgttttgcaa tttgtcgcat ctcatccatt ttgcccatgt
    29461 agtaatcgat cccttgatca tcaccagcac gagcagcatc tgcacgcttc tggttataga
    29521 ggctcatcag ttttttatac ccatctccga tcgtctgttt attttccaaa ctcttttcta
    29581 aatattggat ggcttgatcg ttcacataca attcagaaaa tttaagtcca acttgttcat
    29641 aaagtttcgc taatttatgc ttatcttggg tagtggaaat ctcttgtttc aattgtgtaa
    29701 tctctgattg aatcttttcg gtcgcttctg gcgatagggc tttttcttct tttgccagaa
    29761 cttttttcgg ttctttttct tttttcttaa aaaatccaaa catcattttc cctactttca
    29821 attaaaatgc tttcaaaact gggcttaaga tccaggcata taagagtgct gcggctactg
    29881 tatctacatc ggttgctgta gcattgatga atcccatcga attaaagatc gttactagca
    29941 aagcgggcaa taatgtaata aagaaaccat gagcaatacc gccaatgatc gctcctcggc
    30001 gtccgccaac tgcgttgcca aatatccctg ctgtccctcc ggcaaagaaa ttggttagca
    30061 taccaggtaa aatcatcgct aagccgaaca tgggcaaagt aaacatggca ataatcgtac
    30121 caatcgttgt cgtaatgaaa cctaaaatga cagcatttgg gctataagga aagaaaactg
    30181 gacaatctaa ggcagggatc gcatcaggaa ctaatttcat agctatccct cgaaatgccg
    30241 gcacgatttc acctaacagt agacgtacac cagctagcaa tacatacaca cccactacga
    30301 attggatggc ttgtaaaaag gcaaacatga tatagttttg atctcctgat aattcagaag
    30361 cgaatggttc gcctgcaaat aaaacagtga tgatatatag cgggaccatc acgaccatga
    30421 cagacaaata agtatcttgc agaaattcga aatgtgtcgg caacttgata tcttcgatcg
    30481 actttttatt ttcgcctttc tcaccaaaaa gcttagcaac accggcttca aataagtacc
    30541 cgatcgtaca aaaatgacct aaagcaatat catttgatcc agtgatttta cgaatgatcg
    30601 gttgcgctac tgccggcata gcaatggcga aacaagctcc gataaaacca ccgaccaaga
    30661 tcaatacgat ccctcgcaga ccggcaaaat agccaaagac tgtcgtcatc gttgccatcc
    30721 ataaaattgc ttgaccagtt aaaaaaatgt acttccattt tgtgaaacga gccaaaataa
    30781 tattgaatac aaaaatggct aagaacgtca gtgcaatatc tctacctaaa cccaaatcat
    30841 tcattgcttg accattgata gattcaatag aagggatgat cccttgcata tgaaatcctt
    30901 cagtaaagat tttcccgaaa taagttaaac ttccaacgat gatgctcgaa ccggcactca
    30961 acacttgaaa tcctaggagt gtctttaacg tgccagagat gacttgtcct gctgatttct
    31021 tctgcagtag tagacccaac atagcaatca atgcaatggt gatcgatgcc tgagtcaaaa
    31081 tattttcgat gataaaattg ataacgccca tgtttttacc ctcttttctt tttgtattta
    31141 gataactccc ttatcttgca gaactggggt gattttttct tcaatttcat tttttgaaac
    31201 aatatttttc aaatagatca tcgccgtttt aggatcaact gcgaactttt cgaactgcga
    31261 acgaaaattt tctgctgtga tgatcacatc tggtttcatg gaaactgcgg aagaaatatc
    31321 acaatggtcc agtttggctt ctactcctaa tttgttcaaa acatcctcag cagacatctg
    31381 tgctgcaaaa ctacttccta aacctgcacc gcatacgaat aaaatgttca atttgttcat
    31441 ctttgttctc ccccgtatca tttattattt ctcaactact gaaaaaagca gttgtttgaa
    31501 atcttcgata tttgtgcatt caatcaatcg atccaacttc tcttcgttgt tgatcaattc
    31561 aattagttct ttcatgatgt tcagatgcga gtaggagtct actgctgcca aacaaaaaat
    31621 aatctttact ggatccattt caggattacc gaaatcgatt ggttgctcaa tcgtagctac
    31681 gctgactcct aatttattga ccccatcttc tggtcgggca tgggctagtg ccatgtgttt
    31741 gccaatcacg atatacggtc cgtaatcatt cactgcacga atcatcgctt cgacatagcg
    31801 ctcttcgaca atgttttctt taaccaatgg tctagcagct agttcaatcg attcctccca
    31861 agtagtggct ttttctttga tcatgatatt gctgtccgtc aaaatatctt ttaacatagg
    31921 ctggatctcc cttttgttga tttctaaatt gtttgtttcg aataattttt ctaacttgcc
    31981 atagatgatt cgatctactc ggccaccact ttctttcatg acatctatta tttgagtgaa
    32041 taactccgtc gattcatgac tgttaggctc taaccgctgt agttcttcat ttcttttcaa
    32101 aaagtcttct ataaccaaga tattcgtctc agttaaaatc ggatcgacaa ctaaaagcgg
    32161 cttatttcga taattcaatt gaaaggtgct gaaaaccaga ttgacatcca atttatctac
    32221 taattcgatt tcttttgaac ttaaaactgc agtaatctcg atctgaggaa acttttcttt
    32281 cagattctct gctaacagat ttccagtagc catcccatgg ttacagacaa cgactgattt
    32341 ataaacataa ttcaagtctc gcttgattac actggcgatc gtcgaaaagt ggatgacaag
    32401 aaaagctatc tcgtcctcca gtatcttgcc accaattact tcttcgatcg taggaacaaa
    32461 atgagtgact gcgcggtaga ttgggaaata atttgttcgg atgttttctt tcaatggatt
    32521 catgacttgg atatgattct tgattcttac aatcagtgca gccatgtgct tatagagatt
    32581 ttcgaataaa gcttcttcct ttagatggaa aggtacgtgt gtctcgttct ctacaaattg
    32641 gatcaattgg atcgttagta attgagcttg tacccactcg attgaattgg agatatctct
    32701 agcattgaac gtctcaatag tgaaacgaag ataatttctt tctacttgcg ggacattgtc
    32761 caattgaaat tcctcgatca tgtggtcaat aaatgcagaa aagctactgt cttttatttc
    32821 tgtatttttc caattaatgg cagagataaa ttcatgtttc ctgattcgct ggatccagat
    32881 caatgtaaaa agcaggattt gttttttata aatatcatct tcttgtttca acaacttatt
    32941 ggaatagatc tcctgtattt ttttgatttc caccaaagga aaataatgga gaaagatttt
    33001 ttgagccggt gttaattgtg catcttgatt cataaagaag tcggtcattc ccagattctg
    33061 attgataaaa tcatagatca tcgtacggat agatcgttct gaacctttat aaaccagtcc
    33121 ttgctttcca taactgacga tctctatccc ataactgacc agtgttgctc gtaaccgtct
    33181 catatcttca tctaatgtac ttttagagat ttgaaattct tcttcttttt tgtttagatg
    33241 gatcgggatt cggctgtaag caacggctaa aactagatca aaaatacgtt cgcgccgatt
    33301 caaaaagccg tctttcacat cttttatttc agtttctaat cgagcttgct tttccttttc
    33361 cagcttcaac aaaaaaactt tgttgcgaat tgtacggatc tccggaagct ttttactgct
    33421 caaccagata ttcgcttcaa gaacttcctg acggatcgtc cgctgactga cattgaactc
    33481 acttgctaga tgttttgctt ttactggata ttgggctgtc atgattctgt gaagtatttc
    33541 aattgttctt gttttcatag aaatgatctc cttctgattt caagtatatg gttccgctta
    33601 cataataaaa agtagatatt tctgccactt gaagtggaaa gaaagtaagc aaagcaaaca
    33661 aaaaataagc catatgcttt ttcatctcct caacgaaaaa gacatacgcc tattattaca
    33721 aaaattttgc ccatctatac aaaaaagagg ttgtgaaaaa gctgtcctgc atcaagtaat
    33781 aagaacaact aaactaaaat agtgcttcct atttttcgtt tggttgttct tattatcgca
    33841 gatgcaagct tttaaacacc atttagtgga gaggttgtga cagagatgtt cagccttgag
    33901 cgataagaag cgattttgaa aaatcgcttc ccatatttta caaaatcgtg ccttattgcc
    33961 gataggctat ctctggcaca ctgtttattc agaaaaagga gctgcgacaa ggcttttgtc
    34021 acaactccgt tcataaatat ttcctgtcaa tccagcataa tcggctgaat aaaatcggct
    34081 tgccatcttt tttctttctc agaaaaatca aaataccata tctgagaaaa atactgtcgt
    34141 tgtgtcttac ttcctgagat caatcgccca tcttttcgat cgactgaata atcacagcat
    34201 ctgaaatcaa ttcggatggc aaaactattt tcgtggatct gtcgataatt tcctacttcc
    34261 tgaatagtta tttttttagt atgatttctt acaccgtctg ctttgttttt ttgcagaact
    34321 ttctgatgtt cggcaaacag cttgtctgta taacagcttc tggctttttc aggagtttct
    34381 tcttcccaag cggattggat cttcatgaat atcttttgga tttccagaaa ctgtgctttc
    34441 ttttctttag ccgttcctga catgcgggac aatatctccg ctttcagcct tttcttttgt
    34501 cttttattct tgacgataga aaaagcagaa acccctccta ctgacaaaac agctaatgca
    34561 agaaatcccg tttgctcata actagaagga cctgtacctg gaccataata cccataagaa
    34621 tctctgccgt aagaacgact gccaaaataa ctgcccccat atgaacgacc accaaatgaa
    34681 cctctgcccg aacttcgacc gctagatgta cctccaccat gaccacccgc gacagcgaat
    34741 ccttgggtcg gcagagcaaa caaacatagg cccactaaca aaatgattgc taaataattg
    34801 atttttttca ttgtcttcct cgttatcttt tattacaaaa aactattttt atgatagcct
    34861 tgctaaaagg aagagtcaat aaccagatgc ataaactgtt tttttagcaa ttttgtctta
    34921 tttagtcatt gagtaacatc ggacaaatgt atgtgtcggc agtccataat tcctctatct
    34981 tcagcatgaa caagcttgca gacaagagat ctgcgcttcc tccaggagag atttggtttt
    35041 cctgacaaaa gtgcaacagt gctgtaaatg gacgatttgg taaagcactt tgactagcag
    35101 catgagcaag tttttgcatt tccactaacg ttttctgatc actgcgatgc aatacgcatg
    35161 tgtcatctag cgaactcatc aaaactaaca acatatgcat ttgtttctgt acatatgaat
    35221 cattatctag ctccttagct gcttggattg caagcctgat atgcggatac cctaatatcg
    35281 cttcttcata tgcaccattt acgccatatt tttgcttcac cctatgacca tgagtggttt
    35341 ttttatgacg atattttgta tcaggaaaag cagcaagctt gctgacatca tcaagtattt
    35401 tcgataaaga aaaaggctgt tccatactga ttttttgtgc aatgacactg atcaatagcc
    35461 ccattgccca aatcgctcct ttatgggtat tgatgccatt tgtagcaatc agcattgcct
    35521 tttccgcttt tcgcccaatc tcagcaattc tttctcttaa ctcctgatca atagcatgat
    35581 gccatgctat ttgagccatt tcttcaaaaa aaggagttag cgtcttagca gacttaagaa
    35641 ataaagcata gtccatatcg gaatgtgcac ccgcatccag tgcatccact aatccaggtt
    35701 ttggacttaa tgttacttct gcgatcaatg cttcctctac caacgcagcg agttgctgag
    35761 tcactgcttt ttttcgttcg tcatttatca tgacactcac tccttcttcc tactattact
    35821 tgctgaaacc ttttttgata gctacccaaa tgatatgaag taaccagctt aaagcaaatg
    35881 cacaacttgc cgtcattaga taagctacaa gaatcatgac tcctgaaggt aactgcagcc
    35941 aactatcttt aaacaataat aacagtactt ggaaaacaaa tacattggct aaatacgtac
    36001 gataagcata aacagacaac catttgaaca atggcaattt tggcgaatcg tttacaatca
    36061 ttttggaagc gcctatgaag acagcaaaga tgatgactaa actgtataaa ctttggatcg
    36121 tattcaagta aggtgcattc gcaaagctca agtctcctgg atgtgcgagc aatgaacgga
    36181 cagaaaagaa cgcgataact aaggcaacag gtaagaaaag ataacgtact ctgtccaaat
    36241 attgatagac tttttcgtga taaacaaatg cggctacccc ataaatcatg aaactagcaa
    36301 caaaccgatc taataagtac cagctgtcat gataaggccc agtcaatact tgtgtttgat
    36361 agaacagata ccatgcgaca tataaaatcg ttgaaccaat caatactggc catacatatt
    36421 ttttattagc aaatactttg taaccgagcc aaacaaaaaa cggcatgaac aattggatct
    36481 gcagcatcat aacggtatac cataaatgag gcgctccatc acctgtgaca tatttcagta
    36541 aaaagcttgc agtatccgta tacggagttt gttgttggac tgctggaaat agccaaagat
    36601 aagccgtcgt ccatagaaga tacggaacga ctagttcaaa aaacttttct ttcaaaaatt
    36661 cccaataaga caaatgctga cttgttttta ccatattata aacaattgca aaaataaaca
    36721 ttggtgctgt gtatttaacg atgacgtaaa aggtaccaag aaaagctgta tttgttgcgc
    36781 tgtggctgtt tgccaaagca aagctcaata tcgtttgcat cataacagcc gttacagctg
    36841 atactttctg gaagtcactt acgttgattt tgattttttt gttttcaaac atctctttct
    36901 ctctcttttt ctttattacc aaagttcttt ggttaattta ggtccttcga ctgttttcaa
    36961 tagaattttt ttctctggct ctctcaaata ctcttttagt gaaaaaccgc cttgccgagt
    37021 cactacttgg gtatcgaatt gctcaaaaaa agcaggatgc gattgaatta cctgaattgg
    37081 caattcaatc ggtgaatttg cgtacagcag caaatcaaaa tcactggttt cttttactgc
    37141 cggaatccca gtcgataatt caaatcctaa gctgccgccg attccccatt tacatcctga
    37201 taatatgttt ctagcagcat gaaattgctg ataaacagga taatccatga ttttctcttg
    37261 tctatacgat tcttgttcga tgatgtccca cggatgaatg acttgtttga cggcatgttt
    37321 tgagatttct gttgaccatc tctgattgcg ggcaggtcca cgtaaaccaa tcggtagtat
    37381 ttctgcgtga tccgtcgacg cacgacgaac tgttccgaac atcttttcag ttagaacgac
    37441 ccattcaggc aaggtgaacc cttctatttc atgtttatca aaaaggacaa tatcatgtgc
    37501 ttgaatcatt ttttatgcat cccattcttc gtccattttg gcacggactt tatttgtttc
    37561 gacacgtccg aatgttttgg ctgtttcgtt tgtgtatcga tttgtcagca tcgtgtcatt
    37621 tgtattgcgg acatcttgaa tggcttcatt gattgcttcg atgactttgc tgacattttg
    37681 ttctgaagca gcttggtcgg tgatatcttc taggaaatga tagatcgctc ctagttttgt
    37741 ataattattg atatcgtaag cgatagctgg aactttttca gcagctgctt cgatttcagc
    37801 aaccgaacgt tttgtcacac gtgcagcact ttctttagac atcgcttgga cggtaatttc
    37861 tttattgttt aaagcaatca agcggcttga ttgtaatcca tgtgctaaaa atgcacctga
    37921 aatagcattt cctgcaatca atccaataac tggatgtcca gcttgacgga gttttgcata
    37981 agcagcagca ctattagcca aagccacatg gataccgatc aattcttcgt tatagccgta
    38041 tgcttggctt ggtacatcga tcacaacgat gattggccgt ttcacttttt tgtctttatc
    38101 ttcttcgtaa acgtgatcca aaatttgtcc catgcggaaa ccttcaacta aaccaacttc
    38161 accgtttcgt acacggtaca tcgggttttt aggatcaggt acgattgatg tcacgatggc
    38221 ttcttctatg aatttacttg atttaccata atacacagta ctaatagaag aagttggatt
    38281 tttcatgcca gttagtgccg caaaccaatc gtaaccaaca cttgctgcag ctggttcatt
    38341 tccttctgct acttctggta agtcaattga ttttgcttga tggttagcaa ttgcttcatt
    38401 gtaatcgtca atggccattg gttttgaaag atctaaagaa tcaagtaatg ataaataaaa
    38461 tgctgagctt tcactgcgat gcgtgtcttt tcgttcgtcc attgctgcga tgacagcagc
    38521 tttgattgct tctacactat cagtcactaa ttcatcaatg atttttgatt ctacccgttg
    38581 acgagatcct aatgtattcc aaatcaagtc tttgtcacta gaatcaaatt ctcgaacgcc
    38641 tgcttcttgt tcaataactt ctggtccatt caaaccgact ctcgcttttt ttgttgcgat
    38701 taaataactc attaatgctg aagtaatcga cataccgcca aatgatccta cacgtcctgg
    38761 aatcaaaccg atgactggta catattgttt tagagcaatg attacatttc cgatttctga
    38821 aatagataac agtccgtagt ttgcttcttg caaacgaaca ccacctgtat ctagtacaat
    38881 gactggaaag atctcatttc cttttttatt ttcttctaac acatgttcaa gtgcagcaac
    38941 gatttttgca ccggaaactt caccgatacc gccaccttgg aattttcctt ccatagcgat
    39001 tactactgct tgtttgccat tgatcgtgcc acggctgaca acgatcccgt catcactttc
    39061 agggacgatt ccttgtgctg gtaaacttgg cgcgatgatt tgatcgaatg gatcaatcaa
    39121 ttcacgtcca ttgtcaagta gagcgttcac tcgttctctg gcgtttaatt ctacaaaact
    39181 atttttcatc tgaaagttcc tccattgctt gcgtcaatcg taggtacaca actcctggtg
    39241 tagcaccgaa gtcattgatc acgatatctg ctgaaattgg ataacgatca aaaaatctcg
    39301 ttaatacatt gttccatact tcgccgaagc catcgcttcc tgtacaaatt tttacagtag
    39361 attctgtttt ttctgttgga aacatcaaga tttccaaatc tcctgaaccg accactccta
    39421 catggatttt cttattgatc ggctgtttcg ttggatattt ataagctaat tgttccatgg
    39481 atctctcccc tttttgtaaa acagagcata cacgttctgc ttattcgtct ttcttcttcc
    39541 atctgggtaa atggcgacaa aaggtttttc tcgccaattt tatgaccaag ttctgaattt
    39601 cattggtggt tcatacaggc catctgacca ttcgatcaaa tcatcgatgg tttgtgcagc
    39661 taataatgaa cgtttcgctt cactacgttt gatgcctaga tcttctggta atttcacgat
    39721 gccggctgca cgcaagctat ctagtgattc tttcgtgcta gtcattccaa gaggtgtaac
    39781 accgccaatt gctgcaattg ctgcttgtcg ttcttctttt gtttttgctt tgtacaaata
    39841 agcaattcct tcttctgtca cgatatgtgt agtatcttcg ctataaatca tcactggtgc
    39901 attggctaag cctgcttgtt tttgaacagc tagtgcatct aattcttcga caaagactgg
    39961 ttttttattt gaaccatatg tttcaaccat ttgaacaact aatttttggc ctttccctaa
    40021 tggatcatca ttgtcgcgaa gtgattgcca tgctggtgta gaatgacgac gaccgccagg
    40081 attatgtccc atgttaggcg caccgccaaa accagataaa cgtccttttg ttacggtaga
    40141 agagtttcct tcgtaatcta attgcaaggt tgaaccgata aacatatcga tggcatattg
    40201 tcctgcagct tgtgataatg ttcggttact gcgcatcgtg ccgtctttac cgacaaaaaa
    40261 gacatctggg cgagcagcaa tatatttttc cataccaact tcgccaccaa aactatggat
    40321 gctttcaacc caacctgatt cgatggctgg aatcatcgtt ggatgtgggt tcaataccca
    40381 gttcttcgcg attttccctt ttaaccctag tgattcacca taagttggca gtaacaattc
    40441 gatagctgct gtattgaatc caataccgtg attcaaagat tgtacgccgt gttctgcgta
    40501 gattcctttg attgccatca ttgccattag aatttgcaat tctgtgatgt tttgaggatc
    40561 tcgtgtgaat aaagcttcta attgataagg tttatctgct ttcacgacaa cgtctaccca
    40621 gtcaccaggg atatctacac gtggtaaaga atcgacaact tcgttgactt gaacgatcac
    40681 gataccatct ttgaaagctg cagcttcgat gattgttggt gtttcttccg tattgaaacc
    40741 tgtgtacaag ttgccgtctt tatctgcttt atctgcagct actaaaacaa catttggaat
    40801 caaatcgatg aataagcgtc cataaagttc caaataagta tggatatcac ctaatttcat
    40861 tgtgccgtct tcaatcattt gggcgacacg caagctttgt gcaccagcat aagagaaatc
    40921 gatcttgctt gcgattcctt tttcaaaaat attcaaatgt tctggtcgtg agatactaga
    40981 catgatcaaa tgcagatcat tcactttttc tgcatctact ttttcaagac tttctgaaag
    41041 aaaacttgct tgtttttgat tgtcgccttc taatacaact ttgtcgccgg gagcaatcag
    41101 tttttccatt acttctacga ttttttcagt ttctacatac ttgccattga gcaagttgct
    41161 gattttttcc aatcgctgcg cttttttttc gcgtttagaa gcccagttgc ggtttggttt
    41221 ttccataatt agtcatctcc atttttgata tagaaacgtg gcgtcatcaa taccatgttg
    41281 gtctagattg atcgtacgga tccctttatc tacaccaaat tttgtatgaa tcagctttgt
    41341 caaagtattg ccggggggaa attcaatcgt cagatccatt cctaattctt ttgccaagtc
    41401 catcatttgg ttccactgca cagggaaagc taggttttcc aacaaatctt tacgaatctc
    41461 ttctgcgtcc tgagtggctc ttccatccgt attttttata tacaaacatc tagcatcaga
    41521 tagatggaca gactgcatat acggcttgaa tttcttgacc gtatcattca tcaagagaca
    41581 gtgagatggc acagggactt tcagcagctt cgctgtttgc gcttggtgcc gtttggcttc
    41641 tgccaacgta acggataaac cttcgatact cccagataac gtatgttgca tcggacaatt
    41701 ttcatttgac agataaactg gatggtcagg atcaaatgtt tttgctacaa tttcctcagc
    41761 ttcgcttctc gtcacaccta ctactacgcc cattgcataa ccgcttggat aagcttcttt
    41821 cattaagact gcgcggtgat agactagttc cgctgcattc tcaaatctca aagatcgaca
    41881 agcaacagct gcacaaaaag caccaatcga atgtcctgct gcaatatcag gctgaatacc
    41941 gtttttttcc atttcatcta aataaaatac tgcttttgtc agtaatgcta gctggataaa
    42001 aaccgtatct tgatagtttt cttctttatc aactaaccga taccccgtca cattttctaa
    42061 acgttccatg tacatttcag ggatatccgc taacatccct aacttctgac tgccttgtcc
    42121 aggaaataag aaacctgtac tcatgagcct cgcctcctca gcacttactt tttccactgc
    42181 tatcttagtc aatttctgct ataataaaaa atgccaaaaa aagaaaaaac cataacaaaa
    42241 aggtatggat gggatggata attatgaaat tgcttcatct gcagtatttt aaaaaagtat
    42301 tcgaaacaaa aaatgttacc caagcagcca aagaattatt tatttctcaa cctgccttga
    42361 gccgtgcgat caaacatttg gaaaacgaat taggtgtccc tttgttttac catagcggca
    42421 gaaacattga gacgaccgtt tatgcagaag aattttatcc atatgcagtc aagacattag
    42481 acacattaga agaaggaatc aaattattgt cttcggtcaa tgagaagatc gttacttccg
    42541 tcatattgta tttagaggta gcctctgttt ctattccgaa cttggtccga atttttatgg
    42601 aaaagcatcc tgatatccaa ttaacgatca tgcaacatca tttaccagat gatattaccg
    42661 atcctgtact gtacatcact tcggagatga agcctggcat gaccaatatt cctatcatga
    42721 aagaacctgt ttatgtcgcc attcctaaga aacatcctct tgctaaaaaa gaacatctgc
    42781 aattagaaga tatccaacgc acccccatct tgatgctgtc aaagagcaac gccttccgtc
    42841 atacactgga tgcagcactt gaagcgaaaa gtatcgaatt gacgatcagt tcgacgacag
    42901 atgatcctgc gacattacgt tctattgtca agcaaggatt agctgttagc tttttcccaa
    42961 aagtttcttg gtcttacgat aaaaatgatc ctttcgtctt gcgggaaatc aaagatttgc
    43021 ccttgacacg aacaatttat ttatcttcta gcttcacaga agatcatcct ttgatcaaaa
    43081 tgattgcaaa aacgatgcgg gaattttatt taggaaaata aaaaaaaaag agctgggaca
    43141 aaattcttgt cgcagctctt ttttcgaaca aacggtgttc cagaagtaat ttcttagaaa
    43201 ataagccgaa atttcccaaa aatttgagaa gcaattttcg taaattcctt cttatttctt
    43261 gaagctaaac atttctgtca caacctcttc tctattgtcc tagacattca ttgcgttgat
    43321 ttaaaacttc catcaatcaa atctgctagt tcatctagct tttctatttt acgtagatca
    43381 atagagatcc aatgttcttt attcatgtga taggctggaa aatacccctc ttttcctcgc
    43441 aaaattccgc caagttcagg atcgaccttc aaatcaagaa tattgatgat cctatggtca
    43501 cttgcatcat catagatttt ttcttctgga acagacatca ggaccccaaa ccattttttg
    43561 cttttttgat gtctgaatac gcaataatca ggaaattttt taaaaatata ttctggagac
    43621 aaattgtatg tttcattgat gtgatcgatt agttcttttt tcgttagcat ctgcttccat
    43681 ctctttctta ttgattcgct ttattcaaag ataccacgta agaatagaaa aatgaataca
    43741 agtagttgct aatcaaatac ttcgatattt ttctttttta tcaactctta aatgttcaat
    43801 ccgcctgtgt tggtcttgct accatctact attaaaatgt ttattattgt ttgcccacct
    43861 attttttgat tttagtggta tcatttttca agttcatgat gaaattctta tcatcagtaa
    43921 atgctccaga aaaataaaca atgattgaaa aatcagttga aacttgacca ccgtttattt
    43981 tctccgtatt cttagaatag aagggagtct gattgatgta ttcactaatg aaaaaaatca
    44041 ttaccgaaaa agatattcga cgtcatgttt ctttagtaga acaattgctg aatcatacaa
    44101 agataaccgt aaatgaatta gctgaaatca tagggacaac cgaacgaacg attttttctg
    44161 accttcaatc tattcgctct cagctgcccg aaggctggga catcatcagc gatcaagcag
    44221 gaattagttt gcaaaaccag caaaatttat taacaaatga cctttgggag atttttttca
    44281 aacaatcagt cagtgtcgaa ctgctgaaaa atctgctttt tacaaaaaaa gttgctgtcc
    44341 ctgattttct agccgaccat gggctttctt atgggacatt gaaaagacat gtgactaaaa
    44401 tcaatcagcg tttagcaagt tacgacttgc agatcgattt gacgaaatat acagcttgta
    44461 ttcttggaaa agagcgtgcg attcgtactt tttatcatcg attgctgatt cctttcactc
    44521 ataacaacta ttttttcgag gattattcga tccatgaatc ccattatttt caatttttaa
    44581 gaaacctcag tcaaactgaa ctagcggttg aaacagaaga gattttcgga acatgttggt
    44641 tctttatcaa tacgatccgt ataaaagcga attgccgatt agattcttct atccatacca
    44701 acagcacact ctcctccctg tatgactctg cattgaaaaa attgtattta acggaaggta
    44761 tttatcttaa agacactgag ctttcttttg cttctttctg ctttctggaa agttggaatt
    44821 acaacaataa ctatggacaa gaaaccgctc gctgcctgca tcattctcca tttttagagg
    44881 tcctcgaaac gtttgtagag gaactagctt cagagctgtc tcttgatcaa ctaaaaaaaa
    44941 catctctgac tgataacttg gctctcctca tattaaaata tcatgaatcg ccgatattga
    45001 ttgaagcatt ggaccgtcaa taccatcgtt ttttagaaac atatgatcag caatatgcac
    45061 acttgtatca acagaaagag aatttactga acgatctaaa aaaagaaata gaaatcaatg
    45121 aaccaaatta ctttctccaa ctcttgtctc tactgatcca acaaacgatt ttttctatca
    45181 aaccgaattt gttcaatgtc tattttattt tccaaggaga accttcatgg aaggcgttct
    45241 tgcagcagga attaaaagat tacttaggaa aacgtgtctg cttcttacct atcgaactaa
    45301 ccgatctttc cactatttct tttgaaaaaa cagatatctt gatttccaat ttccctctag
    45361 attctctaga tatccctatt gtatacattt cttctattcc aacgaaaaat gagctcaatc
    45421 gtttgactga actcactttc aaaacttttt tatagtaacg gctagcgact gtattctcta
    45481 gctgtttttt cttttttgcg aaacagtttg ttcatgatcc ctttttctat ttctacaact
    45541 aagaatagaa gaatcacatt tgctccaatc aataactgtt gtgtcatcgt taaacttact
    45601 gtaccgatca gctgctggcc gactggtaga aaaataacca tcccttgcaa taaagcaagg
    45661 atacctaatg aaagaaaaag cgctttgttt tgcaaaagac cttggttcaa tgacggcttg
    45721 gacaactctc tgcaattgat catgtaaaca gcttgagcta agacgatatt ttgcagaaga
    45781 atggtttgct gtaagctttg cccttcaaac tgcatagcca ataaataggc aggaaccatg
    45841 atcagtaggg agacatagat gatccgaaac aaatgataac cagataggat tccttgcttt
    45901 ggatctcttg gtgggcgctt catgatttct ggatctggcg actcgaaccc tagttcataa
    45961 gataaagtga ttgtcgttac catattgacc cataaaatct gtactgggga caaaggtagc
    46021 gggcgattca gcataagtgc ccaaatcaag atcaatcctt gagctaatgc agttggcaag
    46081 aagaaattga tggttttttg caaattatcc aaaatccgcc ggccctcttt tacggctttt
    46141 gcaatcgtat ggaaattatc atctcctaaa accatatcag ctgcctgttt gcttacttca
    46201 ctacctttga ttcccatagc aattcctaca tcggatcttt tcaaagcagg tgcatcattt
    46261 acgccgtctc cggtcattcc gacgatttct ccatttttct gcaaagcttt aacaattcgc
    46321 agtttatgtt ctggtgtcgt tcgagcaaag acatcgacta ttggcacttg ctgaatcaac
    46381 tcttcatcag acataagatc caaatcgatc ccttctaaaa ctttggcagt atgttttaag
    46441 ccaatttgtt ccgcaatcgc ttttgctgtg tctttgtgat cccctgtaat cattttcact
    46501 gagatccctg cttgttggca ttcctttaca gctttgactg cactttcttt tggcggatcg
    46561 atcaatccgg cgatccctgc aaaagttaat tgggttaaag tctcatgcgt gagttcctgt
    46621 ttcgaatcta cttttttata agcaaatcct aaaacacgct gaccttttct cgcaaattct
    46681 gcagctgtct gactccatgc ttcttgttct gcagaagaaa gagttgattt agcaaataat
    46741 acttctggtg cacctttgac gtaaatcacc gctccttttt cctccgcatg catcgttgcc
    46801 atatatttgt attcagaact gaacggaatc tttgcttcta tttttttcaa aggtagattg
    46861 gctgtttctg tgtgtcgaat cagcgcaact tctgtcggat tgccacttaa attttctgtt
    46921 ttctgttctt cttttgtttt taattcctga cagtttttca taatggacaa cacgcaggct
    46981 tcttcttttg cagctacatc cacaactgtc atttcatttt ttgtcaatgt tcctgtctta
    47041 tctgaacaga tcactgtcat agaacctaat gtttctaccg aaggaagtcc tttgatgatc
    47101 gccttttctt ttgccatttc atgtactcca acggatagaa tcatggtcaa aacagctggc
    47161 aatcctttgg gtaccattga caccaccaaa gcaatcgtag ccgaaagaag gatatgccaa
    47221 tccataccat atcgaaatgt cgtaaaaata actaagaata ggctcaaaca aagaatgcct
    47281 tgaaaaattt gtttgtttaa tcggttgatt tttttaatca gaggcgtcgt ttgttgttcg
    47341 accgattgca atgcttgatt gattttaccg atttctgtcg tgtttcctgt ttctacaacg
    47401 acaccgactg cagaaccaga ttgtacaagc gttcctgaaa aagccatatt tttctggtcg
    47461 ccgatcgatt cttcttctgt caaaacggca tgactttttt cagatgcttc tgattctccc
    47521 gtcaaaatcg attcttccac taataaatca tgtgcttcga ttaatcggat atctgctgga
    47581 acgacatctc cggcacgtaa gacaatgaca tctcctttta ccagttcttt cgcagggatt
    47641 ttttcttgca tcccgtttga tagtactatc gcttcttgtc ccatcagctg cttcaaacca
    47701 ttcaatgatt cttccgcttt tctttcttgc cagtaactaa cgagtccatt gacgatcact
    47761 acgaataaaa taatacttcc ttcaatatat tcactagaag caaatttcaa aaaagaggca
    47821 atgatcaaca cgatcatcag cagatcagta aaatgctttg ctattttttt ccatcgtttt
    47881 gtctgctgcc ttgcttttat ttcattatat ccttgatcag tcaggcgttt ctgtcgttct
    47941 tcttgcgaca agccttctgt tgatgtttga tagtttttca atacatcttc taccgtcaat
    48001 ctgtaccagt tcatcctatc ccctcctgtt taattgatac tgttattata cgcagggaag
    48061 ttttttcagg cgtatcgatt atttcagggg ggtctgaaat aattcgtttt gcaaaatgta
    48121 tctgggccta actgatattt ctattgctta ttcttttata tacaaaattt aagaatacaa
    48181 accagataac caataaaaaa ggaataaagc gtgtcgtgct ttcgaataac aagctgaaag
    48241 caattgaagt aaaaaacacc aacaaagcaa gatctttata tctttgtgta gtgttccgtt
    48301 tcttttcttt aataaattga acatgtgcac acaagatcga agaccacaca aatagaaaac
    48361 aactcgttgt catgcctgcc aaaagctgaa aaaacaatcg actatcagct acaaaaaaat
    48421 gtagacaaag accggcaaaa aataacattc ctgtaattaa aataccgtgt aaaggtacgc
    48481 ctttcttaga aagcgcacaa aaccaatggg gcaaactttt ttcttttgct gtttgatata
    48541 aaatcctgct agtactgtag actgcactat ttgctgagga caaagaagca accaaaatca
    48601 ttagattgac aattgctgct ccgccttggc aatggatcac ttgcagcata gtcactaaag
    48661 gactttcttt taaatctaaa caatgccatg gaacaacaaa taaaatcgac aaaatcgttc
    48721 ctatatagaa aaagagaata cgccaaggca gacagttgat tgcttttttc aaattctttt
    48781 gcggatcact tgtctcgcca acggttaagc cgatcatttc aattccagca aaactgaata
    48841 aaaccatagg aaatgagact accattccag caaagccaaa cggtagtaat tcatcgatct
    48901 gtccaaaatt ttctttgatt ccgatagact gggtatgaaa atctgagaca ctcaatccta
    48961 tacccaataa gataaaaagg aaaatcccca agatcttgat gattgagaaa aaagattcca
    49021 attcaccaaa ccacttgact gcaacaaggt tgactgccat taagatcatt agtgcgctac
    49081 ctccgcttat caaagctggt acatgtacaa accagaaatg gatatacaat cctattgctg
    49141 ttaattccgc aagtccagct ccaagccagc aaagccaata agtccaattg acaaaaaatg
    49201 cccacttttt gcctaaataa tagtcaacga aatcactgat agagttaaat gtactatttg
    49261 ctaaaaataa atcacccatg gcccgcatca aaccataaat agccaaaccg catatcaaat
    49321 aaacgagaat aacagctggc cctgctgatg caattgcctt tgctgaacct aaaaataatc
    49381 ctgttccaat tgcgccacct aatgcgatta actggatatt tcgattagtt agagtccgtt
    49441 tcattgccgg tttttccatc aagttcacct ttttcttttt caagcagcat ggcgacttcc
    49501 tgttcccaag aaggtttctt tattttttca atacagagtg gtactacaaa caaaatgaca
    49561 ctgatggctg tgactgcaat tagattgatc catgttccag ctttcatcgt cagtccgaca
    49621 gaaaatccga ttgccgcaaa taaaacaata acgacagtcc aaactccaag attgctcttt
    49681 ttctcttttt ttccaattct aaatggtcga ttcaactttg catgtgtata acgcaatctc
    49741 aaaactgcga tccccatgat gacatagacg atacagtaga taacagtagt tgaatttacg
    49801 agcatcaaaa atgcttgatt gacgccagga atcagcaaat aaaaaagtgc aaagattgag
    49861 ataatgacag actgagtcaa aataacggat ttggatacgt caaattgatt cgtcttccaa
    49921 aaattgaatt ttggtggata cgcacctttt ctagcacttt ctgtcactgt ctttgctggt
    49981 cctgaggccc aagcagataa ttggactgct acaccaatga agacaagtaa actaaacaga
    50041 ttcacaataa gatgtggcaa accaagaatt tggcaataaa tcgaaataga ttgcgcgata
    50101 ttattcaatt ccatttgtcc ttttggtacg acatttgcta caactaaagc attcaaaata
    50161 ttgacgaaaa atataaaaat taacgcggca aatactccgc gaatatacgt tttaactgga
    50221 ttttccaatc gagtgatata cacagaggac atttcgatac cagtaaaaat aaacatgatc
    50281 ggcgcaaaat aaacaaatga ggatgcagtc gtagtatctg gtattaattt gtcccatgaa
    50341 aaactgccta atgttccggt tggtacgatc ccaactttga tccacgcagc aaaccctaat
    50401 aagagcatca tcgtcaaagg cacgtacaat cctaaccaaa caccgacttt tccgccgatt
    50461 tttgccatat caaatttcaa attcaaaaaa gtgatgatcc aataaacgac taaaatgaca
    50521 actaatgtaa atttgctgtt caaaccaagc ggcacattat cgataatatt accaaacaac
    50581 ggagcgagtg ctgaagcgac catcaccatc ccaggaaaca tttggaccca taacagccat
    50641 gagacaacga acccccattt ttttcctaga gcattcgata cccaaagttc tggaccacct
    50701 ttttggggaa acattccagc aaattcacca gagatcaatg agattggaaa agcgtacaat
    50761 aatactgcaa ccagcatgta aaaaatcatc gtccagctcg ttgctgctac atctggaata
    50821 tttcgtacac ttgctacaag ggcgcaggtc attccgataa aggtcatggt cgtgatttgc
    50881 tttgatgaac cagtatcttt catctttatt ccttctttca atagcgacat atgtttttgt
    50941 tttcttttat ttttgttcaa gaacagaaat gacaaggttt catcgattca gtacaaaacc
    51001 cagtatcacc gaaacatcca ttgaacgatc ttagtgaact gggtttgata catgaaacga
    51061 acgatccaac ttatagaacg ttttataaaa aagaatatct tggcgaggaa tatatgaaaa
    51121 acagggggaa atacaattgt tttgatgaag caaataattg tctgttctcc atatgtctct
    51181 tgttattctt tcacacaaaa atcagttcta ttggatgatt tcgtttagtt tcttttccat
    51241 tgctttggcg attttttttg tataaaaatt gaaaattcga tcatcattca aataaggagt
    51301 catgatagcc gcacgtagca gagtgatttt ctgttctttt ttccattctt ctttcgttag
    51361 acccattctt tcaacaaaac gaatcggaga atcaccgtaa tcttcttgtg taaatgtagt
    51421 atgagaagta ataaaccgtt caccataaac atcgccatcc atataagaag accgatcgaa
    51481 cattttttca tttaattcat tgatagcgtt caaatctgta cagccttgtt ctttgaatac
    51541 ccaattgacc atattaaaat caggatgatt caatggatag acttcgattg tttttccttt
    51601 aacagtaaaa gtcagatgat cgagaaattc tctgaatctt tgagctgcct cgattgaagc
    51661 cccaatcaat tgaccatatc cggtcacatt caatggcaat acacgatgtg ctgtccagac
    51721 tgcggcggcg gttgctccag cttttgatcc ttccaaaata taagctccta acatatctgg
    51781 agcctttact gatttttcaa acacatatgg cgcgaaataa gaaatgatat ttctcatatt
    51841 tttatgtttg attacgattc ctccagcagc ataagggaca tagcccatct tatgaggatc
    51901 tatcgtgaca gaatccgctt ctgaaatagc tctgaaccct tcatatacct ctttgtcaat
    51961 cgttacgcag tggtggaaaa catgatgttc ttcaaagaat tcagctaagg aagcatacgg
    52021 cacaaattct cctgcttcgt ttagaaacag tgaacgcgca tagccgccat atgcggcatc
    52081 aacatgtaaa tagaaataga tcccttcatc ttttaacctt tctcttaatt ggacgatttt
    52141 atctacactg tctacttgac cttcttctgt cgttcctacg acagcaacca cacctagaat
    52201 tgggattttt tgtccagcta attcacgaat tgttttttcc aaagcattaa tatccattcg
    52261 ataatcctct tgaactggga tagcaaccat ctgatctaag ccgactccgc aaatatctaa
    52321 cgccttcatc caagaatagt gttttgtttg agggaccagc catttcccta atctttggat
    52381 attttttccg ctacgggaag aagctgcttt gacctcatcc atttcttcgt ccgtaaagct
    52441 ttcggtcatt tccaaaattt cttcaacaga taaattcaaa agttcccact cagacatttt
    52501 tttcactttt tcaggcagca cttccttgac agctaaagga atcgatttga tgcaacgggc
    52561 gtaccataat ccttcaagat tagcaataga accatctgct gcaatgtgtc cccatccgtc
    52621 tgtcatattg aataatgaag caaaatcctg tcctacttct gcttccattt gcgaagttgc
    52681 catagaagat tccaatgcaa cattatttgg gttccacagc atcgcatagt tgtaagctaa
    52741 taaagcaggc atcaatgttt cagcattcat ttgtccccag taacggcctg ctgaatgcca
    52801 tgggatcgat cctgcgcgta atcgctgact gacttcatcc aagacctcta tcatatggtc
    52861 gcgggtggct agataatctg gagtgagctt atctccttcg ctaattgcag gcatatctga
    52921 aggaatataa ttttctctcc atccaagatg ttcatctacc atcttgttca acaacatttt
    52981 ataaactggt ccattttcag ctttgtcgcc tataaagacg gccttgatat ccatatcttt
    53041 catttttctt ccctccatat tttgttggtc aatgtcttgc agatacatgt gtttatctgt
    53101 aacacctctt cactatagcg ccaaaaatga gaaatttttt tttataacat caaatccacg
    53161 tagattttta caaacaaaaa taaataaagt cggatttttc tgataattca acgcaaaaaa
    53221 gcgaacaatt tttcaataaa tcagtcttct aaaatagata acctaaaaaa agcgaacaaa
    53281 caaataaaat gactgtcctt cattcactaa acatattttt atttttttat attgttaaaa
    53341 attcacaatt tattttttaa tagaattatc tgttttccaa attttataac ataaaaaacc
    53401 ttttttaacg gcatttatat gctttttcag aaattaactg atgatttcaa aaagaaaaca
    53461 tgtcataata tttcccatat tgtgcaaaaa aatacaaggc ggaggctcta ctatgattga
    53521 agaatatatt gagaaaaata ttttaagaca gctgttttta tgtgggcaat tctatgtaaa
    53581 taaagaagtc aatctagaaa agctctccaa tctcctgcat gtttgcaaaa ccactttact
    53641 caatgatatc aataacataa aaaaagaatt tgaagaacag atcgcttata cacatcggga
    53701 aaaggaccgt tacactttat atttttcaga acatatacct cgatgcaaga tcatgcagca
    53761 attgtctcaa aattcgttgt ttttaaaaac ttgtctgctt tatttggaag aagatgaacc
    53821 tgattatctt caattaactg aatgtgaatt catctctgtc agcaaagctt attcattaaa
    53881 aaaacaagta ttagcttact ttaatgactg cgggatcgaa atcgaccgct actcccctcg
    53941 attcactgaa atggaaagac gtctgctgct tcttaatgtg tcgtatcgtt taggcggttt
    54001 taattcttgg gagctacctg aatcgttttt tgaacgtgcc gatcgtttca ttgaatccgt
    54061 aacggaaaat agtggcagat tttatgataa agaaaataaa gaaatcttat cgattggttt
    54121 tgctatcagt ttcctacgcc agcaagtgtg tgctgtgaca atagattcaa aatttatcga
    54181 agaaatcaaa aaacgtccta tttacaatta tgtcgaatca gcatgggaaa acactgattt
    54241 ccaaacctat tataaaaaag aagaattcgc ctttattttg acactattca atctttgtaa
    54301 ttatggtttc cattcttatc agttgattgc ggaagacttt caacagctac accaagtgtt
    54361 tattgacaat actcccgaaa taaaagaact agttgctacc tttgaatctc acttcaatca
    54421 agaattattc ggaaaccaac cgttcgaacg tgcgctgatt catctgatgc gttcggcttg
    54481 ggataactat caattattca tgccagaaaa attttatctt ttaaatgagg aacagacaaa
    54541 tttatataaa gaagtccaga caattttttc cagctggtcc agccagttac cttatgatct
    54601 acggttgaat cctaattgca tgcgggcttt tgtgatcgaa ttgagcggaa ttctccgtct
    54661 gacaaaagaa catctgacta tctatatcgt cacaaattcg gatgttcatt atctgattta
    54721 tcgggaagcc ttggaagctg taactacttt cgattttcaa gttgcaccaa caatttattc
    54781 aagtatatct gatataaaga agtatgcgca gcagtcctca aaccgggtat tatgtgaacg
    54841 tacattatat acaccagatg cggtccaata tgaaaatatt attcctattt cgatcaatac
    54901 gatcgatcga gctatcattt cagctgtgca aaacaaataa ggggctgtga caaaaatcat
    54961 gtcacagcct cttatttgtt gaagatgccc atttctgtaa caatctcttg atacacgtca
    55021 ggctcacaac ctcacttatt ttggacatct aggggtccga tctctttatt taaaaaataa
    55081 gaaatgatgg ttcggataac taccaacgcc gctagttgga aaatatcctg caaggtaggt
    55141 ttgataatcg attcgatgat cccagctgaa acaagaattt ccaaacttag aagtatatag
    55201 cctcccaaca tctttttaat agcattgttc tcgcttactc ggtcgctttg cacttttgta
    55261 tggacaaata aatatttcaa aaaatccttg attgatagac aaacacccca aaccaataaa
    55321 cagatagcaa agatatcaag tcccagtaca agtttttcga aatatggaag aagaaactgc
    55381 atcaactctg tcgtcatcct acctctcctc ttctttgttt ttctttgtct tctatttcct
    55441 gattcaagaa ataagaaatg actgttcgaa tgataataat aagtgcaagc ttcaaaatat
    55501 ctgtccaagt cggcttgata actgactcga tgatatcagc aacgactagt acttccaagc
    55561 ttaacaaaat ataacttgct agcagttttc ttgtatctgc attacgagta ttacgttctg
    55621 catagttttt cttgacatga gaccaatcaa aaagtttgaa tagaacaaga acgatcccga
    55681 caattaagat accgatagaa caaatattta aaatagcaat gatccattct agaaattcaa
    55741 ttaaatttga gcgaaacata tacgctacct actttctttt aatttgttcc tattctttta
    55801 ttgtaaaaga aagttacctt gttcgtctat ttaaaagttg ttctaatgat aacggttcgc
    55861 ttaaattttt ctttttcact ctcccatttt tctccctcca tccataaatc gtggaagtat
    55921 ccaaaacaac gtagcaagtt aaaataaggc ttagtccgtc atcagctttt caaagaagca
    55981 ctgtctcggt gctttttttg tacccaaatt tctaaaaata gaaccataca cctcctcaaa
    56041 tcttttcttc ttacctaccc tatttttatt tacaggaaaa tcagacactt cttctttttt
    56101 gtcaaaattt cctttttctt ctcgtcctat catttggcat tctaataaaa acgaactatc
    56161 cttggatata gaaagagaaa acaaggaggc gtttttcatg agtaaaaaat taatcaaagt
    56221 cggcatcggt ttaggtttgc ttgcccttgg cgctgcatat ctaggaaaaa agacaggact
    56281 attcgaagat gacagtcatt tatatgatga attcgaatca atctagcatc ctatttagtt
    56341 gaatcactac ggacatactt ggaagacatt ggaggaatca caatgagaaa aagtaaagca
    56401 ataatgattg gtgccggtat tgccaatatg gcagcggctg tttatttgat tcaagaaggc
    56461 aaatggaaag gggatcagat caccttttat tcattggatg atcacggttc aaatgatggt
    56521 gcaccgaccg acactgtagt ggatgaatat tggaataaaa accatccatt agaaaaccaa
    56581 aaaggctata ttgctcgagg cggtcgcatg cttaattacc gtacctatgt agatttgatg
    56641 gatctgttaa gtcgaatacc ttctgttact gagccaggga tgactgctga agaagatacg
    56701 agagattttg atgcaaaaca ccgaaccttt gacaaagcac gtttgatgga aggtggcaaa
    56761 ggaatcattg atggcggaaa actgggattc aataataaag accgtattct tctgactaaa
    56821 ctgattgcta tgcctgattc agaagaagaa aaactagaca atatcactat cgaagaatat
    56881 ttcaaagatg atccacactt ttttgaaaca aacttctggt ttatgtggga aacaacattt
    56941 gctttcagaa cacgtagttc tgctcaagaa ttgcgtcgct atatgcatca aatgatttat
    57001 gaattcactc aaatcgaaca tctagtgggc gtcaatcgaa cacgttacaa tcaatacgaa
    57061 agtattatgc ttcctttgat cgagtacttg aaagaacaag gatgcaaaat cattttgaac
    57121 cgccgtgtca ctgactggga attcaaagaa acaccgatgc aagatgaaat cactgtgact
    57181 ggtttgaaaa ttaccaatac actgactcaa gaagaagaat acgttgtggt cgatgaagat
    57241 acagcagtta ttttcactaa tgggtccatt acagattcag ctactttagg agattacgaa
    57301 acaccagctg tagaaaatat ggattatggt gcagcttcta gtctctggaa aaaagccagt
    57361 gaacgtttct acaacttggg aaatcctgac aaattctttg ctgatcgaga tgcaagcgaa
    57421 tgggtcagct ttaccctgac gacgaaaaac catttgttgt tgaatgaaat cacccgtatc
    57481 acgactcaag aaccaggaaa tgccttgaat tcatttattt caaccacacc gatcacgccg
    57541 ttaggccaaa aagatgtcaa tatgtctatt gtcgtgcatc atcaaccgca ttttacgtct
    57601 cagaaaccaa atgaaacagt tatttggggt tatttccttt atccaagacg acgtggcgaa
    57661 tttgtggata aacagtatat taaaatgaat ggaaaagaaa tgctagaaga actgattgga
    57721 caactttcaa aagtagatcc tggaccagtg aatattcgtg aaaaagaaac agaaattttc
    57781 gacagcgtaa taaataatat ccctgtctat atgccttatg cttccgcgtt gttcaataat
    57841 cgtgccaaaa gcgatcgacc aaaagtcatt cctaaacatt cgaccaatct ggcctttaca
    57901 ggagaatttg tcgagcagcc ttatcagatg atctttactg aacaaagtgc tgtacgttct
    57961 ggagaaatcg ctgctttcca ttttgcgggt attcctatgt cccgtcttgt caaaacaccg
    58021 cgttacgaca aagatattcc tactctagct cgtgccgcaa aaaaaatgtt tgaataatca
    58081 agaggttgtg aaaaagctgc cctgcatcaa gtaataagaa caaccaggct aaaacagctt
    58141 cttatgtttt tgatttggt
//
LOCUS       DACDYI010000009        57233 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.009, whole genome shotgun sequence.
ACCESSION   DACDYI010000009 DACDYI010000000
VERSION     DACDYI010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.009"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            281..973
                     /locus_tag="IWM52_000515"
     CDS             281..973
                     /locus_tag="IWM52_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP9972795.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            1181..1471
                     /locus_tag="IWM52_000516"
     CDS             1181..1471
                     /locus_tag="IWM52_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972796.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            2185..3555
                     /locus_tag="IWM52_000517"
     CDS             2185..3555
                     /locus_tag="IWM52_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972797.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEI
                     NRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELI
                     FSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLI
                     FLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLF
                     TELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLI
                     MGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFI
                     ITVINR"
     gene            complement(3795..3878)
                     /locus_tag="IWM52_000518"
     tRNA            complement(3795..3878)
                     /locus_tag="IWM52_000518"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3842..3844),aa:Leu,seq:gag)
     gene            4261..5478
                     /locus_tag="IWM52_000519"
     CDS             4261..5478
                     /locus_tag="IWM52_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9972798.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            5662..6498
                     /locus_tag="IWM52_000520"
     CDS             5662..6498
                     /locus_tag="IWM52_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9972799.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            6574..7722
                     /gene="nagA"
                     /locus_tag="IWM52_000521"
     CDS             6574..7722
                     /gene="nagA"
                     /locus_tag="IWM52_000521"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP9972800.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            7942..9000
                     /locus_tag="IWM52_000522"
     CDS             7942..9000
                     /locus_tag="IWM52_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAP9972801.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            9019..10038
                     /locus_tag="IWM52_000523"
     CDS             9019..10038
                     /locus_tag="IWM52_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAP9972802.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(10140..10856)
                     /locus_tag="IWM52_000524"
     CDS             complement(10140..10856)
                     /locus_tag="IWM52_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP9972803.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(10825..11610)
                     /locus_tag="IWM52_000525"
     CDS             complement(10825..11610)
                     /locus_tag="IWM52_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9972804.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(11601..12350)
                     /locus_tag="IWM52_000526"
     CDS             complement(11601..12350)
                     /locus_tag="IWM52_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAP9972805.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            12648..13172
                     /locus_tag="IWM52_000527"
     CDS             12648..13172
                     /locus_tag="IWM52_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972806.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            13188..14687
                     /locus_tag="IWM52_000528"
     CDS             13188..14687
                     /locus_tag="IWM52_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAP9972807.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(14775..15377)
                     /locus_tag="IWM52_000529"
     CDS             complement(14775..15377)
                     /locus_tag="IWM52_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9972808.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            15481..16437
                     /locus_tag="IWM52_000530"
     CDS             15481..16437
                     /locus_tag="IWM52_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAP9972809.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(16742..17698)
                     /locus_tag="IWM52_000531"
     CDS             complement(16742..17698)
                     /locus_tag="IWM52_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9972810.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            17872..18432
                     /locus_tag="IWM52_000532"
     CDS             17872..18432
                     /locus_tag="IWM52_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972811.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            18425..19249
                     /locus_tag="IWM52_000533"
     CDS             18425..19249
                     /locus_tag="IWM52_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAP9972812.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            19259..19876
                     /locus_tag="IWM52_000534"
     CDS             19259..19876
                     /locus_tag="IWM52_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAP9972813.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            19894..20577
                     /locus_tag="IWM52_000535"
     CDS             19894..20577
                     /locus_tag="IWM52_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP9972814.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            21260..22495
                     /locus_tag="IWM52_000536"
                     /pseudo
     CDS             21260..22495
                     /locus_tag="IWM52_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            22503..23390
                     /locus_tag="IWM52_000537"
     CDS             22503..23390
                     /locus_tag="IWM52_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9972815.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            23407..24243
                     /locus_tag="IWM52_000538"
     CDS             23407..24243
                     /locus_tag="IWM52_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9972816.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            24246..24869
                     /locus_tag="IWM52_000539"
     CDS             24246..24869
                     /locus_tag="IWM52_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972817.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            24866..26158
                     /locus_tag="IWM52_000540"
     CDS             24866..26158
                     /locus_tag="IWM52_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9972818.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      26517..26614
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            26730..27311
                     /locus_tag="IWM52_000541"
     CDS             26730..27311
                     /locus_tag="IWM52_000541"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP9972819.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            27314..28621
                     /locus_tag="IWM52_000542"
     CDS             27314..28621
                     /locus_tag="IWM52_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAP9972820.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            28914..29402
                     /gene="purE"
                     /locus_tag="IWM52_000543"
     CDS             28914..29402
                     /gene="purE"
                     /locus_tag="IWM52_000543"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAP9972821.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            29386..30519
                     /gene="purK"
                     /locus_tag="IWM52_000544"
     CDS             29386..30519
                     /gene="purK"
                     /locus_tag="IWM52_000544"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAP9972822.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            30532..31827
                     /gene="purB"
                     /locus_tag="IWM52_000545"
     CDS             30532..31827
                     /gene="purB"
                     /locus_tag="IWM52_000545"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAP9972823.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(32007..32384)
                     /locus_tag="IWM52_000546"
     CDS             complement(32007..32384)
                     /locus_tag="IWM52_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972824.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            32640..33368
                     /locus_tag="IWM52_000547"
     CDS             32640..33368
                     /locus_tag="IWM52_000547"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAP9972825.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            33368..33622
                     /gene="purS"
                     /locus_tag="IWM52_000548"
     CDS             33368..33622
                     /gene="purS"
                     /locus_tag="IWM52_000548"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAP9972826.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            33624..34295
                     /gene="purQ"
                     /locus_tag="IWM52_000549"
     CDS             33624..34295
                     /gene="purQ"
                     /locus_tag="IWM52_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAP9972827.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            34296..36518
                     /gene="purL"
                     /locus_tag="IWM52_000550"
     CDS             34296..36518
                     /gene="purL"
                     /locus_tag="IWM52_000550"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAP9972828.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            36503..37942
                     /locus_tag="IWM52_000551"
     CDS             36503..37942
                     /locus_tag="IWM52_000551"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAP9972829.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            37974..39017
                     /locus_tag="IWM52_000552"
     CDS             37974..39017
                     /locus_tag="IWM52_000552"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAP9972830.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            39014..39592
                     /gene="purN"
                     /locus_tag="IWM52_000553"
     CDS             39014..39592
                     /gene="purN"
                     /locus_tag="IWM52_000553"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAP9972831.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            39589..41124
                     /gene="purH"
                     /locus_tag="IWM52_000554"
     CDS             39589..41124
                     /gene="purH"
                     /locus_tag="IWM52_000554"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAP9972832.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            41140..42414
                     /gene="purD"
                     /locus_tag="IWM52_000555"
     CDS             41140..42414
                     /gene="purD"
                     /locus_tag="IWM52_000555"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAP9972833.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            42536..43450
                     /locus_tag="IWM52_000556"
     CDS             42536..43450
                     /locus_tag="IWM52_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAP9972834.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(43583..45148)
                     /locus_tag="IWM52_000557"
     CDS             complement(43583..45148)
                     /locus_tag="IWM52_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP9972835.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(45141..46592)
                     /locus_tag="IWM52_000558"
     CDS             complement(45141..46592)
                     /locus_tag="IWM52_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9972836.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(46606..47622)
                     /locus_tag="IWM52_000559"
     CDS             complement(46606..47622)
                     /locus_tag="IWM52_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9972837.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            47842..48930
                     /locus_tag="IWM52_000560"
     CDS             47842..48930
                     /locus_tag="IWM52_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP9972838.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            48984..50510
                     /locus_tag="IWM52_000561"
     CDS             48984..50510
                     /locus_tag="IWM52_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP9972839.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            50512..51717
                     /locus_tag="IWM52_000562"
     CDS             50512..51717
                     /locus_tag="IWM52_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9972840.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            51974..52951
                     /gene="guaC"
                     /locus_tag="IWM52_000563"
     CDS             51974..52951
                     /gene="guaC"
                     /locus_tag="IWM52_000563"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAP9972841.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            53215..54639
                     /locus_tag="IWM52_000564"
     CDS             53215..54639
                     /locus_tag="IWM52_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP9972842.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            55100..56209
                     /gene="pdhA"
                     /locus_tag="IWM52_000565"
     CDS             55100..56209
                     /gene="pdhA"
                     /locus_tag="IWM52_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAP9972843.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            56212..57189
                     /locus_tag="IWM52_000566"
     CDS             56212..57189
                     /locus_tag="IWM52_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAP9972844.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT        18451 a         9702 c        12410 g        16670 t
ORIGIN      
        1 cgttttttgt aataaataca gtaatctatg aaatgaaaag ggttacgttt aataaaactt
       61 gcttttttgt ttttgttcac cataaatatt tatttaagaa agtattaacc tgcatttttc
      121 tctataaaaa agatttttta aatagataaa aaaggaggat taaatgagta tttgatagta
      181 tttatattaa aattacacat gtaatttaaa aacgcttgac attgtgtttg agatcactta
      241 cttttagcat gtaaaagtat tggattaagg agctatgcaa atgaaaaaga aatttattac
      301 tggtgggatt gtcgttgcat ttctatgtct aattggctac acaggcacgc ggtacgcaaa
      361 tgaaattatt ttgtggggtg gagagacaga tattgataca atcaatgaga atctgacaca
      421 gcttgatcat acattgacag ataaagagca agcgcttaca gatcttaccg ctcgactagc
      481 acaaaaagaa cagacattgc aagattcaaa ggcagatgtc caagaatacc aaacaaaagt
      541 aaatgaatta gaaaaccaaa aaaatcaatc ggcagcagaa aaacaaaact tagaaagtca
      601 attagtcagc aaggatgcac aactacaaag caaacaaaat gaaatcaatg tcaaactaga
      661 tgagatcaac caaaaaaatg atgaaatcaa tcatctgaat gcagattgga cagcaagact
      721 acaagaagca caagcaaaac tttcagaatt acaaaatact gtaaatggtt tgaatcaaca
      781 aatcgcaaca ctgacacaag aaaaaacaaa tgtagaaact cagttaacag atacacaaaa
      841 gaaattagac gaagcaactg ctgaaaactc aagcttgaaa caatatattc aaaagttaga
      901 aaaagcaaaa gatgaagtgg aagatacagc tgataaatct cagcaaattg ttgaagaaca
      961 tttgcgtaaa taatctttct taacaagtaa acctaaatta gtaaagaagc aacagtaatc
     1021 tatactgctg cctctttttt ttagtacatg tccttcatct tcgcaggaag tgccaatatg
     1081 attttaagaa aggtaataga caaaaagtca aaaagataaa acagagtttc ttctttacaa
     1141 agggaatgta tgttcgtata atggtattga ggtgaaggtt atggagaaaa aatatggaac
     1201 agtagataag atccgtactt tgcaagttag tgccagaccg ttgatccgtt tcagtttaaa
     1261 tggggtcaat tgtttgattg cttcccatag tttgaacttc ctagctgaag tagacgaagg
     1321 gatgaagtta gtcgttacag gttattataa taatagaaag caatttgttg tgagatccta
     1381 tcatgtgtta gggaaaccca aaattgtggt agaatatgag aaaagtctat acccaagaaa
     1441 aaaggtccga caagatgaaa gaataaacta gaaaacaagc ggctgagaca aaaatgtcct
     1501 agccgcttgt tttggaaaga tggatgcaga cgatgggatt tgaacccaca cgagcatacg
     1561 ctcacaagaa cctcaatcta tatataacta catgtattta agtatttgtg atgaatcact
     1621 aaactttcca aactcatttg agggatattg cttaaagatt gaacagatca attcgaagcc
     1681 ctattagtat tcgctcaatg gggggatcag aggccataaa aagagactag tcattactag
     1741 tctcaggtag tggaattgta ggattttaaa cgttcctgga gagtaccggt atgtaactcg
     1801 aagaggtgat tatcatagtc gtaaaagtaa attgaagaag cctcgccttt cacacgagaa
     1861 cgtcctggtt cgacagttaa acctaacatt tggatttttg agacaaacga atctatatct
     1921 tgttcgtcaa ttttaaaagc aatatgattg tatgtctttg gcagtttatt cgacgaattc
     1981 tgcattactg caatccatag gtccttaact aagaaaaatt tttctggata taatgaaaat
     2041 ttttttgttg actatcgtag atttcttttg catcaaataa ggtatttaac aattaagtat
     2101 ttaaaagata acttttaagt tatctttaag taatgcttaa agctatgtta tactctatta
     2161 ggaaatataa gaaggagtat aaaaatgaac aaacataata gaaaaataac gaataacaaa
     2221 gaactgatct tgttactaat agggatttgg ggtatctttt tactctttaa tatgtatatg
     2281 ccgactatga gagcggatga cgtagtttat gcaagtagat tagatgaact tggctatttg
     2341 ggtgcttcga tagaacatta taagacatgg agttcaagaa ttattataga actattctta
     2401 atgttctttt ctaaacactt tatgctatgg aaattattaa attctacgat tatgttagga
     2461 attgtagtat tactttgcaa gtatgttttt gagaaaataa atgttaaaaa tttattattg
     2521 gtttgctcta tatactgttt aataccattg acagtaatgg gggaaaccgg ttggagagca
     2581 accaccttga actatcagtg gccagtagca tttagtttac taacttttta tccgtttttt
     2641 caactcttaa gaggagaaga aataaataga aaaatatatt gggtaagtat tcctttattg
     2701 atatttttaa ctaatcaaga acaagtcaac gcctgttttt ttgttctaac aagtatagtt
     2761 agtttatatt tgattgtcaa tggacgatat aattataagt tatcagtatt ttcaataata
     2821 agcttagcag aattaatttt ctctttgaca actccaggga atgcattacg tgctgcacat
     2881 gaaattaata aatggtttcc tgaatataaa aatttcaact ttcttaataa gttagattta
     2941 ggtatttcat catttggaaa gccgtttttt ttagacatga atatcttatt tttattgcta
     3001 tttttcttaa ttttccttct aacatatagg aaatgtcaaa actattatgt gcgtatattg
     3061 actgcattac cgtttttctt aaacctgatt atttattttg gtaatacaat gggtcaaagc
     3121 tttacttacg tcaatggaaa taaaagggca atgatttgga gtagtagtaa cctgaataat
     3181 ctctttacag agttaggaac gaaactgtca cttttttatc caggtacttg gatagctact
     3241 ttagtagtct tggcattatt actatgttta attgttggaa tttatttaag ttttgacaat
     3301 aagaaaactt ctattttttt agttattttg atgataatgg gattttgttc aaggttaatt
     3361 atgggatttt ctcccacagt atgggcttcc ggaatgcgga cgtactatat actttatgtt
     3421 gttatcgcca tcttagtact aatggcagtt aaagagctaa tgaaaagcat gagtgtacaa
     3481 aaaaatgaat ttatgcaatt tggattaacc gtgcttggta tttgtacctt tataataacg
     3541 gtgataaaca gataatatac aaaaaatccc tcgctttagt attaggtgag ggattttact
     3601 cgtttttcga ttaattgttt taacacttgc aagtttctac gagtagcttg tttccgacct
     3661 tcatatactc acctcaggct gtttacatga ttgtacgttc tttttgtaaa ggatacagtg
     3721 atatgtatat aatggcataa agaaacgttc aaacaaaaaa actcgtgaag caaaattaac
     3781 ttcacgagtt gtagtgcaga tgatgggact cgaacccaca cgagctaacg ctcacatgac
     3841 cctcaatcat gcttgtctgc caattccaac acatctgcgt aataataaca cactaatgat
     3901 agcgaggttt tacatagttg tcaagctcaa aaatgggagg acttattaag atggagaaat
     3961 atgacggaga attttcagga ctgggaatga tcttaggtgt attgattgga cttgcattcg
     4021 gacgtttttt gcttggtttt atgctaggaa tcatttgtgg aattgcaatg gattgggcag
     4081 cgaatctatg gaatgactat catgacaatt aagttctcaa cgcttgtttg tgttttttta
     4141 atacttacgt ttttcctaaa taagtggcaa aattttctag gatggagtat gatattgaaa
     4201 tagtaaataa caaaaaattt ggaaagttct aactttttga aatgagggat tggctaagtg
     4261 atgagagaat ttgaaaaatc caacaaatta gaaggtgtca gttacgatgt tcgcggtccc
     4321 gttctagaag aagcggagcg gatgcaagaa gagggcatta gtattttaaa attgaacacg
     4381 ggaaatcccg caccttttgg atttgaagca ccaaacgaag tcattcgtga tttgatcatg
     4441 aatgcccgtg actcagaagg ctactcagat tccaagggga tcttttctgc tagaaaagca
     4501 attgagcaat attgccaact gaaacatttt ccgaatgtga cgatcaatga tatctacacc
     4561 ggcaacggag tgagcgaatt gatcacgatg tgtatgcagg ggttgctgga caatggagat
     4621 gaagtactgg ttccaatgcc ggactatccg ttgtggacgg cttctattgc attagctgga
     4681 ggcacacctg ttcattatat ttgcgatgaa gaggcagaat ggtatcctga tattgatgat
     4741 atcaaatcaa aaataacgag tcggacaaaa gctattgtta tcattaatcc taataatcct
     4801 actggggcac tttatccaaa agaattatta gaagaaatcg ttgaagtagc tcgacaaaat
     4861 aacctgatta tttattccga cgaaatctat gatcgcttag tgatggatgg tctagaacat
     4921 attccgattg ctacattagc gcctgatttg ttcgttgtta cgctgaacgg tctatccaaa
     4981 tctcatcgtg tagcaggatt tcgagtaggc tggatggtcc taagtggaga taagtcacac
     5041 gtcaaaggct atatagaagg gctgaatatg ttgtcctcca tgcgtttgtg ttccaatgta
     5101 ttgtctcagc aaattatcca aaccgcgcta ggcggttatc aaagtgtcga tgagctgctc
     5161 ctgccaggcg gaaggatcta tgaacaaaga gaatatatct ataatgcaat caatgatatt
     5221 cctggtcttt cagccgtaaa accaaaagct gctttttata tattcccaaa aattgataca
     5281 aagcgattca atattttgga tgatgaaaaa ttcgttttgg actttttaca tgaacatcat
     5341 attctacttg tacatggtgg tggatttaat tggaaccagc cagatcattt tcgaatcgtt
     5401 tatcttccta aaatggatga tttgaaacaa acagctaaga aaatgagaga attcctagca
     5461 acctatcgcc aaaaatgatt ttaaaaacca aatttttcag taggagggcc acagacaaac
     5521 acactgaggc tctctttcta gtggagtttt cttaaataac taaagtatat tatttagttg
     5581 caagtctttt cttgaagttt gcgacttcct aaagtaaaat acgaatagaa tgcgaatacg
     5641 catattttgg ggaggatact tatggaaacg ttaatcgagt cctttggaac gatcattaaa
     5701 gagattcgaa aagaacaaaa aatgacgcaa aaaatgcttt cgcatgatat ttgttcacaa
     5761 agtgtattaa gtcggatcga aaataatgag gaactgccga atgtattggt gatgatgcag
     5821 atttgtcaac ggctaggggt aacgattgat catgtgatga atctctctcg aaaaactact
     5881 cggacaaacg ataaattatt tgaactgttg gatagttgtt tccaaaaacg tgattatcgg
     5941 aagttagaac aagtactaaa atcttctgat atttctgaaa atctgtatca agcaactgac
     6001 tttcaacgct attattatta tctcggtgtt tgcgaattca cgttgcgtca aaatatctct
     6061 cagtcccttt attatttgaa agaagcatta agctactcta gccaaaaaga aagaatccat
     6121 gtttctgaca cagaaataca actgattagt tgtatcggaa aaatatatgg tgtggcagga
     6181 aaaacggctg aagcacgcta ctatttgagt cgaagcatcc aactgctgca tcaatcagct
     6241 gatgaacgat caaaaagtga attttcgcaa attttttata attatggaaa tttcttattc
     6301 catcaaaatg aaatcgacga agcgttagaa caggtgaatc aaggaatcta ttgggctcaa
     6361 gagaagaata gctactatta cctcaatgat ttatttgtgc ttaaaagctt aatatacaag
     6421 cgtaaagaag aaccagaaag agcagcattc tatgaaggct tagcacaagc tgtgaaacaa
     6481 atttcaaaaa atttgtaaat ggtgtagacc attcttgact tcttctctaa ataagagtac
     6541 tttagaggta gaaaaacaaa gggagagaat agaatgagaa cttttatcta tgctgacaaa
     6601 ttctttttag cgtcaggtgt caaacatgca ggctatcttg aaattaccga tggacttttt
     6661 ggtgaataca ccaaagaact acctgaggga gaagtcaaca tcattgacca aagcggcaaa
     6721 tggattgctc ctggtttggt agatacacat attcacggct atatgaatca tgatgtaatg
     6781 gataatgatg cagaaggcat caaagtgatg tctgaaggtc tgttatcttg tggagtgact
     6841 tcatttttac caacaacatt gacgtcaagc aaagaacgtt taaaagatgt agctgagaca
     6901 atcggcaaaa tgtatcagga agcaccagga gctaaaatca aaggaatcta ttttgaaggt
     6961 cctttcttca cagaagaaca caaaggggca caaaacccaa gttatttcgg ggatcctgat
     7021 ttggatactt tccatgaatg gcaagaagct tcaggtggtt taatcaaaaa aattgcttta
     7081 gcaccggaac gtcaaggtgt agaagagttt gtcaaacaag tgactgaaga aggagtagtt
     7141 gtttctctag gacacagtaa tgctacacta gaagaagcgc aaaaagctgt tgaagcaggc
     7201 gcaagtgtat ttgtccatgc tttcaatggt atgcgtggat tgaatcatcg cgaaccaggt
     7261 atggtgggtg cgttattatc tttagaacat gtcttttctg aattgatttg tgatggccat
     7321 catgtacatc caaatgctgc gggaatccta atggaaaaag caggacatga tcatgtcgca
     7381 ttaattacag actgtatgat ggccggtggg atgcctgatg gaaattacaa tttaggcgaa
     7441 tttccagtag tagtaaaaga aggaacagca cgtctagaat ctggcaactt agctggaagt
     7501 atattgaaat tgaaagaagc aattaaaaat gttgtagact ggggattagc aacaccagaa
     7561 caagcagtca tgatggctag tctgattcca gcaatcagct gtaaaatcga tgatacatgt
     7621 ggaaaaatcg ctcaaggacg cgatgcagac tttatcgtat tgaaccctga tatgacattg
     7681 gaagcaactt atctagatgg cgtggaacgt ttccacgcat aatataattt aagttaacct
     7741 taaaactaaa cttcttaagt ttggttttaa ggtttttttg tgttctagta aaaatcactt
     7801 tacataagtt tcaaaagttt ctttaatttc ctattgttca ataagtaaca atgtaaagat
     7861 aaaattaaaa atgttaatac gtattagcaa aataaaagct ttttttattg tgaataaatg
     7921 aacataatta ggaggagaaa gatgaactta tttgacattt ttcgatcgac tttgacagat
     7981 atgtcgattg tcagtgcaat cacttcaaca gtttttatta tattattagg gtttgtttgt
     8041 agaaaaaagg ggattttttc tgaatctgtt ggaaaaattc tatcgaaagt cgtcttaaca
     8101 gtggctttgc cagcactggc atttcgagct ttcatgcaag atatcaatac agaattactg
     8161 atgcaaggaa tcaatgtctt gatttgggga ttagttattt atattattct tattctagtt
     8221 tcaaaacctt ttttcttaag ctataaaggg gataagcaaa caacgttgcg tgtcctgacg
     8281 atctttggtt ctacgacctt tttcggaacg cctatcgtca gtgcgattta tggtcctgtc
     8341 ggcgtgatgt attcctcaat tttcaatatt ggctaccgaa tctttttgta ttcttatgga
     8401 tacataaaaa tgagtggctt gaagatggaa gctaaaaata tcaaagcgat gttcgtcaat
     8461 ccaatcatta tcgctacttt tgccggcctt ttcatttggc tgttccaagg gtatctgcca
     8521 caagtgtcag ttacaggtgc agaaggagag ataagccaag tagcgttttt gcgaattgac
     8581 cagactgcac cgtggctatt tcagccgatg gattatttag ctggtttaag ttcaccattg
     8641 gcgtggctag caatcggcgc aacattaggt gaagtaagct tcaaaaaagc tgcatctgaa
     8701 aaaacttcat ggtattacag tctagtcaaa gtggtatttg tacctgcatt gaatatcgtg
     8761 attctagcag ttttgacggc aacgaatatt ctaccagtca attttgaagc actggcaaca
     8821 atcgttatca tgatggctac accagctgca acagtggcag cagcctatgc tatcagtttt
     8881 gatagggagg cacttttagc ctccaatgca tccttactgt caactatttt tgctgtggta
     8941 ctgacacctg tatggatcgt tgtattacaa atgattcatg gcataggttt attttcataa
     9001 gaaatggagg aagaaagtat gagaatcgca tgttacggtg ttcgtccgaa cgagatagat
     9061 ttttttgaaa aattgaatat ttaccattat gatctcagtc tttatgaaga actgttgaca
     9121 catgacaata tcgtgtcagc aaaggaccat gatgctgtat tgttgagggg aaattgtata
     9181 gcagatgaga ccaatcttgc aaaaatgcag gaatatggga ttcgttatgt ttttacacgg
     9241 actgtgggag tcaatcatat cgatctacaa gcggcagctg attttggtat gacagtagcc
     9301 cgtgtaccat cgtattcgcc aaatgcgata gcagagttat ctttgacttt tgccatgatg
     9361 cttttgagaa atactgcgta tacgacgatc cgaacatctt tcaaagattt tcgagtggat
     9421 gagcaaatgt ttagtcgtga aatcagaaat tgcacagtag ggatcatagg aacaggcagg
     9481 atagggttga cggaagctgt tttgtttaag ggactaggag ccaaagtgat cggctacgat
     9541 attcatcaga cagaagctgc taaaaaagta ttgacgttcc actcgcttga agagttatta
     9601 gccaaaagcg atattgtcag tattcatgta cctcatatcc caggtgaaaa cgatcaaatg
     9661 atcaatgaag gatttttagc tcaaatgaaa aaaggtagca tcttgattaa tacagcccgt
     9721 ggagagcttc aagataatga agctatttta agagctttga aaagcaatca tttagctggt
     9781 tttgcaaccg atgtttttgc aaatgaaaca gaggtttttt tccgatcctt caagccatgg
     9841 gagacgattc cagaccctgc tatccagcaa ttagtagaaa tgtaccctag agttcttgta
     9901 accccgcatg taggttcaaa tacagatgag gcattatcaa atatgatttc gacaagtttc
     9961 gagaactttc gtgaaataat agaaactgga aaaacgaaaa atgaggtatc tcttcctaaa
    10021 gcccgtcaat taagataaga aaacatggat attcgtcaat aaaatgttta aaaatagtcc
    10081 aagtaaacag ttgttacttc tgtatacttg gactattttg tatgggataa ttcgatgact
    10141 caacagtaat ctgtgagctt tttccgccca ttctttgtca attcataaca ggttagcggc
    10201 tggccgattt ccaaatattt gattctttcg ttcaatatat cttgttctac taaaaaattc
    10261 aaatatttct tgacggatat acgtgacagt gagctttttt gagccagttc ttttgttgaa
    10321 aaaatagtat cttcttttag aataaatgca cttatttttt ttaaagtcag ttttgacaga
    10381 cctttaggca aatcgatgaa tgatggtatt tcgtgaatgg gagtggtgtg gttgaatact
    10441 tgatcgatca atttctgatc ggctttttca gtcgtttgga ttgtctgaga gaaagcaaga
    10501 tacttttcga tcgccagctg aaaacgttgc aaagtgaacg gtttgatcaa ataatctaag
    10561 gcaccgtagc ggatcacatt ctttatggtt tcgatatcat ctgcagcagt gatcaaaatc
    10621 actgggatat catttccttg ctgtctaagc caagataaaa aatggatacc actttcgcca
    10681 ggcatgtata catcaagcag cagcagatct gcgttttttt gtctgaaata tttttttcct
    10741 tcttctacat ttctgaaact ttcgattacg ttcatattag ggatgctttc cacaaaagta
    10801 cgattcaatt ctgcaaccat tggatcatct tcgattatca ggacatttgt catcattttg
    10861 atcttccttt tttggtaatt ctagatagac gattgtaccg ccatttgttt gtttggaaaa
    10921 ttctaaaaat ccaccgtggc gtttgacgat tgaatggatg agattcaatc caaacccacg
    10981 attgattccc ttcgtcgaaa aacctagcgt aaatattttt tctgagattt cttctgatat
    11041 cccgacaccg ccatcgcaga cggtcacgat gaatacttca gcttctgtgt catagtttaa
    11101 atgaatccgg acctgtccgt ttctttcatg tttttgtagc acggcttctt ttgcattatc
    11161 aagtaagttc cctaacactt gcaaaagtga atggatggag tcatccatct ccattttagg
    11221 gataaaagaa tcgctttcta acagcatttc aacagattgt tctttggctt cgtttatttt
    11281 gcctaataaa aaaccggcaa ttgcaggtac tttgatcaga cttgagactt ggccaatctc
    11341 ttttttataa ttttgattta gctgctggat aaaatgggtg acttcttcat acttcttcaa
    11401 atcaatcaaa ccagaaatga catgaagttt attcatgaat tcatgggttt gagctctcat
    11461 ggagtttata tattgtttcg tccccccaag ctcctccatt aatttcatta cttctgatcg
    11521 atttcgtaaa gttgcgactg ctcctaaaaa acggtcattg ctaaaaagag gtgtaacgct
    11581 agaaatagtt tctatcccat ttaaatacat ggattgagtc cgaacggtcg tttgatgctc
    11641 aatcgtctct ttaaacaaaa cgtcatatag attatcactg atttttttcc tacttcgaaa
    11701 tccaatagct gctctgtttg gtagaaaatg ttttttgcag gtgtattgat caagaggata
    11761 gttttatcag aagagatggc taagattcct tcatctactt cattttcgat aatcgttttt
    11821 tcacttaact gttctgctat ttcttgtggt tcaagtccaa gcagtacttt tttcattttt
    11881 tgggcaagaa aaacggcgcc tatgatgcct acgctaagtc caataatcaa tgaaaaaaca
    11941 atcatttttt gtccatcttt gacttgattg tcaatcgtgt tgtttgttaa gccaacacaa
    12001 actgcaccaa taatctcttg atcataataa atagggacga aatagcggaa gccgaaccct
    12061 aacattcctt ctttttcaga gaaaaattct tgtcctccaa tcactttttt cacatcttca
    12121 tgacttgaga aaggaagacc gatgtaactt gggtctgcgt gagaatatcg aatgccttca
    12181 gggtccatga caacgataaa atctaaattc gatgcaatca tgatttcatt tgttattcct
    12241 tgaatcgcat gtgtcgggtc accagtctgc agcttttttt ggatgagttt atcattggca
    12301 acgattttag caatgctaga gattttatct gacacactag atcggttcat cgaaagaata
    12361 aaattcctga tcaatatggc agaaacaatc aaagaaacga atgtggagaa acaaacgaca
    12421 aggatgatcg tgttctggag ctttaatggt tttttcttaa aaaaaggaaa cataggctac
    12481 ctcgaaagaa tttattgtga aaattttaac atgaaaacaa aaatttaaca aattgtcaac
    12541 gataaacaca accttcctat aaaaaacacc actgagtcgg taaaagaagc tatacagaat
    12601 tttcaaacat tggtatgata gacgtagaaa tggaaggagg cgtgtatatg atagggggaa
    12661 tcgcacttga gacgatttat tggtatacat tgctgatttg tgcaggattg gcagtcctat
    12721 tgattatttt tggagatgta ttcgattttg atggcccgct tgatccgatg cttattatcc
    12781 cttggttaac tttcacaagc ttatttggtt ttctaggtga gcgtttaaca gatcaatcat
    12841 ctttattttt atttattgct agtggaggca tatcgatagt gttggtattt ttgcttaatt
    12901 tctatatatt gatgccgatg aaacatgccg aatcaacgat gtcgtcttct gaaaaaactc
    12961 tggaaggaca agtagctgtt gtgatcacac cgatccctgt acgaggtatg ggagaaattc
    13021 agttgaaaag tgttaccggt tccattagtc gtccagcttg tttttacagc ccccaagaac
    13081 agacagctga gcggggaacg aaagtattga tcattgagat caaggaaaga atctgttatg
    13141 tcatgcctta tgaaagcatg ataaaagctt aaaggagcgt gtgttaaatg gaaataccag
    13201 ttgtttttat cgcgatcgca atcgcagctt tgattttact gatgttgctg atcgtctttg
    13261 tagcgaagta ccagacagca aaaccggatg aagcgttgat catcagtgga agttatttag
    13321 gaagcaaaaa tgtgcacatt gatgaaagca ataacaaaat caaaattgtg cgtggaggtg
    13381 gcgcattcgt cttgccagtt tttcaacgtt cgaatcggat cagtctatta tcgagtaaat
    13441 tggacgtctc cacccctgaa gtgtatacgg aacaaggcgt accggtgatg tgtgatggta
    13501 cttcgatcat caaaatcggt tcatctgtag aagagattgc tacagcagca gaacagtttt
    13561 taggcaagac aagagaagaa ctggaaaatg aagcaagaga agtattagaa ggacacttgc
    13621 gctcgattct tggttctatg actgtagaag aaatctatca aaatcgcgat aagttcagtc
    13681 aaagcgtaca agaagtcgct tctgtagacc ttgcaaaaat ggggttgatc atcgtttctt
    13741 tcactatcaa agaggtcaga gacaaaaacg gttatcttga ctctttaggg aaaccaagaa
    13801 tcgcccaagt caaaagagat gcagatatag cagaagcgga agctctaaaa gaaacgcgta
    13861 tcaaaaaagc tcaagcagaa aaagaatcac aagcagctga attacagcga caaacagaga
    13921 tcgcagaatc attaaaagaa aaagaactga aattagcaac ctacaaacaa gaacaagatg
    13981 ttgctaaagc aaaagcagac caagcctaca atttagaaag tgcccgagca caacaacaag
    14041 taatcgaaca ggaaatgcag attaaaatcg tagaacgcca aaaacaaatc gaactggaag
    14101 aaaaagaaat cacacgtcgt gaaaagcaat atgattcgga agtgaagaaa aaagcagatg
    14161 ccgatcggta cgctaaagaa caagaagcac aagctcaaaa agtcaaagaa gtgactgaag
    14221 ccgaagcaga acgattccgt gtggaagcac tggcagaagc ggaagccaat aagacacgtt
    14281 tagctggaca agcagaagcc gaagcagcac tagcaaaagg aaaagcagaa gccgaagcaa
    14341 aacaaaagat cgccaatgcc ttcaaggaat atggcgaagc agctgtcttg agtatggtta
    14401 tcgatatgct accgcaattg atgcgcgaag cagcacagcc gctaggaaat atcgaaaaaa
    14461 tctctgttgt cgatacagga agcagttcag gagaaaccag tggagcgaac cgagtgacaa
    14521 attatgccac aaatttgctt tcttctactc aagaaacact aaaagcgaca acaggacttg
    14581 acttgaagga aatcgtagag aatttgtcga cacgtgggac tactagccaa gtaaatatgt
    14641 atgaagcgcc aaaagaggca gaacaaaact cgatgaagga agaataaagt agtacacttc
    14701 accgttcaat caaaaatgga ggaagacttt ttaaagttct tctctccatt tttttatttt
    14761 attgcaaaag aaaattagtc ctcgctattt ttcttttgtt tttggatctt agcttctatc
    14821 tcttgtaaat ttgttcttag caatcgttct ttcttttgga gttcgaccaa atcttctgat
    14881 agttcttcct tcttttctgt ttcaggagga cgaagaaaga aattgatacc gattgcaatg
    14941 aatgtcccta taaaagtatc tagtacacgt tggatagcat acaaagcgga ttcgccttga
    15001 gggatgctca acgcaatcag caacaacgta gctattgcgg aaatgatacc ggaattattg
    15061 ttaataccat ctgacacgac gatcacaata ataactagaa taggaagcaa gatcaattct
    15121 accaaaaaat cattgtgaaa atagcttttg acaaaaaaat aaatgatagc taatgtacca
    15181 cctaatgtat tgccgatgat cctagacttt ccaaaagaaa cggttgtagt caagtcctga
    15241 cgcaaagaaa aaacagcaga taatgcagca atcatcggga ttcctcgttg agtcaaatga
    15301 aaaaacaaga tacataaaaa aacagctaag gcacttttga tcgtacgcat acctagtcga
    15361 aaacggccaa attgcataga gattctcctt tttggtcttt ttactatcat acctaaactt
    15421 ttgtctgata gctagttttt cattattcaa gtataattaa aataaaaaag ggggaagtaa
    15481 atgcgtatag gaatgcgaac agtcaaaaca gtgatcagtg tcttcttttc gatggtcaca
    15541 gcgggtagct tatcgctgtt gtattggcca gctgcaggga tcattgcatt attaagtgta
    15601 ggaaatacca agcgctctac attgcgtacg ggcatgaatc gaatcgcagc ctttatatta
    15661 gctacggcaa cagcactgtt cagttttttt gctgttgggt atactatttt tggcttcagt
    15721 ctttttttat tcctgttcat cccgcttgcc tctcgcttca aattaaccga agggatagta
    15781 gtaaattcgg ttttggtcac tcattattta gtggaggaaa acatgtcttg gcagctgatc
    15841 ggcaatgaag cgggattaat ggcgattgga ttagtatttg ctttgcttgc aaatgtttac
    15901 atgccagatc gtacgaagca gctgaaggaa aaccaaatac agatcgaaaa agaatttcga
    15961 aatattttga gacaaatggc agaatttctt ttatcagaaa ataaagaaga tgtgcagatc
    16021 aaatgcgaac atttactgac attcattcgc gaaagccaag aagatgccag agaacatcaa
    16081 gaaaactact ggctcagaca gccgctatac tacgaaactt acttctctat gagaagagca
    16141 caggccaatg tgattaaaga catgctggaa aatctagaga gaatccagca accagcttat
    16201 tatggaaaac atatctatgg tttattgatt tatactgcag aaacattttc agagtcaaat
    16261 gatggcagac aaattttaac acgtattgaa gaagtttatg tattgtatcg gcaaatgcct
    16321 ctgccgacta gtcgccctga attcgaggat cgagcagaat tgttccagtt tttgcagtct
    16381 tttaaaagtt ttattgaaat caaggcagaa ttctcccaac aaaaaatcca aaaatagagg
    16441 ttgtgaattc agctaatatt caggtcaaaa agctgatttc acaacctctt ctttaaacgg
    16501 tgttctcctg tcaactcctc ggtcacaagt cacaatattc aacaaacatg agaagctgtt
    16561 ttttgaaatt gctccttgtg actcggagct gaacggtagg ctcacaacct ctttcttaaa
    16621 cggtgttcaa aagctttcct ctgcggtaat aagtccagcc aaacgaaaaa taggaggagc
    16681 tatttttgtt tggctgttct tattacttga tgcagaacag ctttttcaca accttttaaa
    16741 attacatatt tttccataga acttgcattt gatccaagat ttcgatgaca tcttttgtca
    16801 agaagagaga gcgattgggc aagtcacctt caatcatgtg taccatattc tttatttcgt
    16861 aattcattgc ttcagatgta ttcccgcttt cgatgaattc ctttgtacca tctgtaaata
    16921 agatctccgc acgatcagca cgcgggtaat cagcaatcgt cacataaccg ttctcaaagg
    16981 caacgatacc gacttttggc atttttgcct gaaaagtcaa gctgacagca gcaagttcat
    17041 tttctttgtt acgcaagata gtgacggatt gttcgtccac gcctgtttca aaaggaacca
    17101 ttgtagaggc aactacttca ggttgagagc tcaaaaagaa acgagcgaaa gatacagcat
    17161 acgtaccgat atccaacaga gctcctccag ctaattctgg attgaagaac cggtttttag
    17221 ggtctggttc tttgtaactg ccgaaaggtg cttggatcat ttttaaagcc cccagttttc
    17281 ctgtatccat tattgagcgt aactgctgat aaagtggcat gttgaagatt gtcatcgctt
    17341 ctgctaagat caaattcttt tcttctgcta agcgcatagc gtcagccagt tcttttttat
    17401 tcatcgtgat ggctttttca catagaacgt gtttgcctgc ttccaatgct gccaagatat
    17461 ggtcgatatg ctgtctattt ggtacagcaa tgtacacgat gtcgatttca gcatcgtcta
    17521 acatttcttg atagcttccg taagcttttg gaatattgta acggtgagcg aagttttccg
    17581 ctttttctga agttctggaa gcaactgctg ttagttcgct cgtctcttga ttgaagcttt
    17641 cggcaaactc atgtgcgatg gtaccaagac caacgattcc ccattgatat tttttcatct
    17701 gaaaacgctc cttcatagtt ggattgagtt tcttgtctgt ctattattat aacaaaaatc
    17761 acgaagaata aagaggagag gacttgaaac attaaaaaaa atgaaagcag cgatttaaga
    17821 ggtgcttttt gatcaaaact taagtagaat agttaaaagg aggggataaa cgtgcatcaa
    17881 gtatatattg atcttattat gagtgtatta ttagaacagt ttgaaactga aaaaaatttt
    17941 ttagatgaat acttgctgat cagtccagaa caatggcaaa aatggaaaga tggcgagaaa
    18001 aatctgccat ctgaagcgat gcagaaagta aaaaatcttt tcactgatta tgaatggatg
    18061 ctaacgcaaa agattttgcg tcaaacgatt atttttcccg aaaaacgaaa cgttgccgtt
    18121 gcagagtaca agcaaatcaa aacacgcatc gcgcaaaaat ggcttagcag caaagcagca
    18181 aaagtcgagc tgatcccttt gaaaaataat cagcaggctg gtagatacct tgacttgaaa
    18241 gtaagtattc aatatgatga atggggctat gatgatattt tgaattttcg tctgccagca
    18301 gatgttcaaa aacagatcga aggagaaaaa atcgaacttc tcgattgggt gaatgaaaat
    18361 ctggaagaaa cttacgttca tgaggcgaat ggggaacgtg aagaacaacg aaaggatttt
    18421 tcaagtgaat aaatttttag gatttggagc agttgtgatt atgatcgcag caatgggcta
    18481 tgttattttt agcgatgatg attcgcatgt tcaagccggc aatcaagcag tcgaacgaac
    18541 aagcaccaaa acaactgcta gtaaagaaaa gaacagtcaa acaaccagca gcaaggtaaa
    18601 ggacaaagaa acaaaagatc gtacgtccaa acaattgcca gatgttaaaa cagacgattg
    18661 gaagctagtt ttggttggtc cagatcacaa attagaaaaa gaaattgatg agtctactca
    18721 attggcatca ttaagtaacg gatacttgat cgacaaacgc attgctccag aatatgaagc
    18781 atttgccaag gctgctgaag cagctggatt tccattagtg atggtctcag cttaccggtc
    18841 tgtatcatcc caacaacaag ttttcgaaca aaatgtccaa gatgtgatga gtcgtcaagg
    18901 tgtatcagaa gaggaggcga caaagattac taaagaaacg attacggagc caggatacag
    18961 cgaacaccac acaggtctgg cagtcgatgt cgtcgatcaa aactggtaca acagttatcc
    19021 ttctactgta ttggatgcaa gttacggaga tcagcctgga gctaaatgga tcgcagataa
    19081 tgctgcgcag tatggattta tcgttcgtta tccaaaagga cacgaagata taactaagat
    19141 cacatatgaa ccttggcact tacgttatgt cggtaaagaa aacgctgagt atatcaccaa
    19201 acaccaattg acgctagaag aatatctgaa tgaattaaat gaaaagtagg aattgatcat
    19261 ggcgaaaaaa aagtggaaaa agcaaaagcg caagaatatc cgttggccag cagtggttgc
    19321 cggtgtagca gtgatactct tagcctttgt tttttccttg aagaatctgt cttctccttt
    19381 tactgataat cagcaagaaa atgaagcgca atcccatcag caatttattg aacggcttgt
    19441 tccgcatgcc caagaactac aagatggtta tggagtgctt cctagtatca ttttaggaca
    19501 agctatatta gaatcaaatt ggggaaaaag taccttggct agcaaataca ataatctttt
    19561 cgggatcaag gcttatgggg atcagaagaa agtaagcctg gaaacaaagg aatttgttaa
    19621 cgaagaatgg ataactatcc aaggagactt caaagtgtat gattcatggg aacagtccat
    19681 ggatgaccat acacaactgt tcgtccaagg agtagattgg aatcctgcgc tgtatgaaaa
    19741 agtgattacg gccaccaact atcaggaagc cgcacaagca ttgcaagatg caggttatgc
    19801 gacagacccc ggttatgcgc aaaaaatcat ccaggtcatc gagacgtatc aattgaatca
    19861 gtatgaccgg agatagcaaa ggagaagtgg ctgatggcag aacaatttac gccagaaatc
    19921 atgacggaat tgagaaaaga tatcgtaaga gtaccggaaa tcattttaga agcaagcggg
    19981 atcaagatat ttggaaaatt gatccgttcg atcatcttta ctacggatat tgcgatcata
    20041 cgtaatacaa atgcgaatgc agtaattgct gtgtatcctt ttacacctca tccagcgatt
    20101 actaaaagca ttattgaggc agctgacatt ccagttttct ccggtgtagg tggtggtttg
    20161 acacaaggtg ctcgttcttc ctacatgagc atgttcgcag aagcgcaagg ttcgttaggg
    20221 gtcgttttga acggtcctac gcctttagag acagtaaaag aagtttgtcg agtagttgat
    20281 attccagtta tcagtacggt aacgtcgaaa tatacagaaa tagatgaaaa gttagcagct
    20341 ggtatttcga ttatcaatat cagtgcaggt aaagaaactg cagaagcagt acgccatttt
    20401 cgtaaaaaat atcctgaact tcctatcatt gcgacaggtg gtccaacgga agaaagtatt
    20461 gctgagacca ttcaagcagg agcaaatgcc atcacatata cgccaccaag tagcggagag
    20521 ctttttagtc gtaaaatgga aaaatatcgg gaaactgaga aaaagaatca tgaataacag
    20581 gtgagaatca aaagacgagg aagagcagat agtggctctt cctcgtcttt tgtactccag
    20641 tactatttgt tcagaattca aaaaaaagag aaattttttt gtttttttac ctgtaatgta
    20701 cataagaaga gttaaaacct tttgttatta ttagcattct aacaaaaaaa tgtcttgatt
    20761 tattgaagcg ctttctatag aatgaaaaac gaataaaaac taaaaaaggc acccgacaat
    20821 ttagtagcag ctaaattgtc ggggcttgaa aagtcagcgg gaactgacca atcaagttgc
    20881 catagtcaat gcttgtgcat cgacgtgtat attgtaccta atttcataca gaattactag
    20941 ttatttttct gttttttagg gaagagcgca cacgaagaag catgcttatt tagctatgtc
    21001 aaggatattg ggcagttgtc tgatatcctt ttttctttgc gtaattatcc tcccatcatt
    21061 gaagcaagaa gtgtttttag cgatgaaaga agaatcagca ctatctaatt tttcttgaaa
    21121 gtattgagtt gttcatccta ggcagaacga taaggcaagt cttggttttt attccccttg
    21181 aaaaagatcg tttaataaat atgttctgta taaaaataca gtatattgaa aaataacaat
    21241 ggaagtagag gtgcagagaa tgaagaaaag aatgagaatc atcttagctt taagtgttat
    21301 gttattcttc ttgacggctt gtgggcaaga agatgacaaa aaaatcacgc tgaacatggc
    21361 ttggtggggt tcgcaagtcc gacacgatgc aacagtaaaa gtaatcgagc tttatgaaga
    21421 aaaaaatccc catgtgacga tcgattatga attttatgat tatgaaggat attacacaaa
    21481 attaggtaca ctagcagcat cagatgatat ctgggacatt ttccaactga acaatcaatt
    21541 tcctcaatac atcaatcaga ttgaaccatt agatgaatat gtggagcaag ggataattga
    21601 tatttctaat gcagatgaat cctatttagc aacgacgact tacgacgggc aattagttgg
    21661 actgtctaac ggggtgaatt cgtttgcgat tgcttacaat aaaaatatat ttgaacaatt
    21721 aaatatccct gaaccagctt caaattggac atgggatgaa tttgaacaaa tcagctatca
    21781 aatcactgaa gaattaggaa tctatgctat ttcaagattc gaagatttta tagctggctg
    21841 tattattcaa ataccacaag ttgagaaagg attgaatttt tataatcggg aagatctatc
    21901 taacagcttg ggatttgaaa atccagacta tctaacagat tttttccagc tgcgtaaaaa
    21961 tctggtagac aaaggggccc atccagaacc aggtggtgga gctgcaagta cgacggatct
    22021 gtcttttcag gcggttaggg atagagaagc ggcaatggtg tttctatcaa gtaaccagtt
    22081 gacagaaata ttgtcagggg ctcctgaaga tatggagatt cagttgatta atccacctcg
    22141 cagaaaagca gatggagaat ctggtatccc tttacgttct tcgcaaatgc tgagtatggc
    22201 aaaaagctca gcccataaag aagaagctgc taagtttatt gatttctttc aaaacagtga
    22261 agaagccaat gaaattttaa aaggagaacg tggtgtacca atcatgtcga atattcgaga
    22321 acagctcgct cgtgcagatg atgcaacttt gcaagcgaca tttgattatt tagaaatgat
    22381 ttccgatatg gacaacggcg aagtgaatgt ctttgatagt gctttgaatc cggaaattca
    22441 agaccaatac aatcttttag ttgaaaaagt catttatgaa gaagtaacag cagaaggaag
    22501 gcatgaaaaa agaaaaacta aaacggttct tggatcaaga caatgtggtc ggttacgtat
    22561 ttgcttctcc attcatccta ggatttctat tgataacagt gataccgatg atcttgtcac
    22621 tttattattc gttcaccgat tatcgtttgg gaaatccagt ctcatggatc ggcttggata
    22681 attatataaa gatgctgagt gatgcccgtt ttttgaactc catccgagtc acttttctct
    22741 atgtatttgc atctgtacct gtgaaactga tctttggtct attgattgct tatcttttaa
    22801 cgaggaatat caaaggaacg actttctttc gttctttgta ttatgtgcct agtttgatcg
    22861 gtggcagtat ttctatcgct ctagtttggc gggagttgtt ttctaaaaac gggttagtga
    22921 atattttcct ggaacgatta ggcgcacctg cgctttcctg gtttagcgat caaaaactgg
    22981 tcatgatccc tcttgttttg atgtctgctt ggcaattcgg ttcggcaatg atcatctttg
    23041 ctgcaggtat caaagagatc ccaggctcgt attatgaagc ggcagagatc gatggagcga
    23101 acaaatggca gacgttttac aagatcacgt tacctcttct ttcgccgatc attttgtata
    23161 acttggttat gcagacaatc gcggcgttta tgacttttac gcaggcattt atcattaccc
    23221 aaggtggtcc gaatgatgcc acgaactttt atgctcttta tgtgtataac caagcttttt
    23281 cttttaattc tatggggtat gctagtgcta tttcgtggat catgctgttg attatgggag
    23341 ctgtcacctt tctgattttc aaatcatcaa aaaaatgggt cgttgtgtga atgagaggag
    23401 gaagaaatga ataatacagt aacgaaatgg tttcaatacg gttttatttt gctattaggt
    23461 ctagtcatga tttatccggt cttatggatg atcgcaggtt cttttaaaac ggatcaagag
    23521 attatgagtg gttcgctgaa tttgattcca gagacttttc aatgggaaaa ttacgcaaaa
    23581 ggatgggcgg ggtttgctgg aatttcattt tttactttct ttaggaattc gtttgtcatc
    23641 tctgcagttt cgacgatagg gacagttctg agctcgactt gtattgctta cgcattatcg
    23701 agaatcgatt tcaaaggaaa aagattctgg ttcgtcgtca tgttagtaac gatgatgatt
    23761 cctgcacaag tgattttgat tccgcagttt attatttaca atcggctgaa tttggttggg
    23821 acgtatgtgc cgctgatttt accacatttt tttggacaag catttttcat ttaccagatc
    23881 atgcaattca tggtcagcat ccccaaagag ttagatgaat cagcgattat tgacggatgc
    23941 agcaagtata gtgtctttac caaaattatt tttcctttgc tgaaaccttc gatcatcacg
    24001 acgatcatca tccagtttta ttggaaatgg gacgacttca tgggaccatt gatttattta
    24061 aacaagccga gatcttacac ggtatcgatt gccatcaaat tatttgcaga cgctggatct
    24121 agtacgactt acgcgagtat gtttgcgatg tctacgttgt cgctgctacc ggtttttctg
    24181 attttcttat tcttcaataa atatcttgtc caggggatca gtacaagcgg tctgaagggc
    24241 tgaatatgaa aaagccaata gaagagatgt ttctttacaa gctcaactac tatgtctgtc
    24301 ggattttttt aaccagtctt atcagtaatg ctcttttcgc tttagttcta ggatgtttgt
    24361 tcactttagg attagtgctg tctgctcgtg ttgtctatct gatttatttt ttgatgatgt
    24421 atccttctta ttatctatct gtccattact tggcagaaaa aaaggtatgt tggaggaaag
    24481 ggaaaatcaa acggatgtgt ttcatcatta cgttgtttca gctgtttgtc attatttttg
    24541 cagttgattg gtatttatta gtgggaaaga ttggagcttg gtatctgatt ccttttctat
    24601 tgtgtctgtt gctttttgta tcccatagtt tttttagtac gctaattgat atctgcaaag
    24661 aagataaagc agatagtctt gtttatttga tcaaaaggaa tatctggctg ttgtcaaaaa
    24721 acgtcaaggg gtttctgtgg tatttatgcc ttttgtatgg actgttctgg tgtatacagc
    24781 ctgatttttt actcactgca ttactgtatc cagggctgcc atttgtcaca gtattttgct
    24841 taaaagaaaa ggagaatgac tttaaatgaa gaaaaaatat gtacaagtcg gacttggtgg
    24901 gagagcgcgt tttttttatg aggcaattgc ctctacgtat catcaaactt gtgaactagt
    24961 cggtttttgt gatgtatctc aaacaaggat ggattatgca aatcggatac ttgtgaacaa
    25021 gtatggacag aaaaaagtaa agacatgtat gccatcacaa tttgagcaac tactggatga
    25081 cgtacggccg gctgaagtca ttattacgtc agtagatcga acgcatcatg attatatcat
    25141 tcgtgcaatg aaaaaaggat atgatgttat ttgcgaaaaa cctctgacta tcgatgagga
    25201 aaaagctcaa gaaataataa cggtccaaaa acagacggga agaagcctgc gagtcacttt
    25261 taattatcgt tatgcaccgt atcacacgaa gataaaggaa ctgatagaaa gtgatgtgat
    25321 tggtgatgtg ttttctatcc acttcgaatg gctgttaaat acgcaacacg gggcagatta
    25381 tttcagaaga tggcatcgaa ataagcacaa tagcggcggg cttcttgttc ataaatctac
    25441 ccatcatttt gatctagtga atttttggtt gaacagccgt ccccaaaagg tatttgcatt
    25501 cggtggatta aacttttatg ggatgcataa tgcggagaaa cgcggagtag aaatatttta
    25561 cagtcgatgc tatggaagcg aactagctaa agctgatcca tttgctatag acctgtcagc
    25621 caatgaggaa ttgcgagaaa tgtatttaga agcagaaaaa gacagtggat atattcgtga
    25681 ccgcagtgta ttttccgatg atatctctat tgaagataca ctaggcgtaa cggtcaaata
    25741 tgaaactggt gcgattttga cgtattcgct aaacgcgtat atgccgtggg aaggattcaa
    25801 tgtttctttc aatggcagca aaggacggat cgaaatgaaa gtgatcgaaa agtcttacat
    25861 caatgcaggt ggagataaaa aggatgaagg agcagccgtt cttcagtcca tccatgtcta
    25921 tccgatgttc ggtgagccat atgaggtaat cgtcgaacaa gaagcgggcg gacatggcgg
    25981 aggagatccg cgtttattag atgatctatt tggtgaagaa aaagaagaag atccgtggaa
    26041 tcgggcagca actcacgtag atggtatttt gtcgattttg acaggaatcg cagcaaatca
    26101 ttctattgct tcaggaaaag cagtagtgat tgacgagctt gtcagcttaa aagaataaga
    26161 atagatttac ggaaattact tcccgtagtt tataaaatct tgccttattg ccgataggct
    26221 gtcttttgaa cactatttat tcggaaaaag gggctatgac atgctttttg tcatgccttc
    26281 tttttatgtg ggctgctttt ttagatagga gaaaataaaa ataactattt aaacccgaac
    26341 aaaaaggaat aatgcattaa aaatcattag attttacaaa aaaataagta aaccacgttg
    26401 tcagtttcag aaagtagtgt tagactgttt tcaatatttg aaacaacatt ttttattcaa
    26461 atatggcaaa ctgccttcac gtgcaaaaca gttgcaagaa aatgaattta cactattatt
    26521 agttttacat acctacttat atatcgtcat aatatggatg acagtttcta gccagtaccg
    26581 taaatgctgg actataagta aaagattggc tatttcaccc ttcttttctt atgataaagt
    26641 ggagggtgtt tttctttaac agaaacatag taggtttttg cacaaaagcg gttatttcgg
    26701 tattaataaa tgataaagga gcaaataaag tgaaagaatt agttgaacgt attcaaaaag
    26761 acggacgtat attaggcgag ggcgtattaa aagtcgacag cttcgtcact caccaagtag
    26821 accctgtatt gatggaacaa atgggaaaac ggtttgcaga agtatttaaa gaacaaggaa
    26881 tcacgaaagt tgtgaccatt gaagcttctg ggatcgcacc agctttattt gctgcaagag
    26941 aattagatgt tccaatgatc ttcgctagaa aagcaaaaag tttgacgatg gatgaagaat
    27001 tattgacagc ttctgtttat tcttttacga aacaagtgac aagtacgatt tctatttctc
    27061 gtaaattttt atcttcagaa gacaaagtat tgattatcga cgatttcttg gcaaacggac
    27121 aagcggcaaa aggattgatc gaattatgcc agcaagctgg agcgcaagta gaaggaatcg
    27181 ggatcgtcat cgaaaagtct ttccaagatg gacgtcagtt acttgaagaa atgggattga
    27241 gagtcgtttc tcttgcgcgt attgcttcct tgacgaatgg acaagttgag tttcttgagg
    27301 aggacgcata atcgtggaaa aagaaacaca aaatggaaaa gctgcggttt tagggctgca
    27361 gcatctgcta gcgatgtatg caggagcagt agcggtgcct cttttgatcg gtacaggact
    27421 aggctttaat caagaacaga tgacttattt gatttcaatt gatatcttta tgtgtggatt
    27481 agcgacatta ttgcaattga cagttaaccg atttttcgga attggcttgc ctgtcgtttt
    27541 aggatgtgcg atccaagcag tagctccact gatcttgatc ggaaccaatg aaggagtagg
    27601 agctatctac ggatctatta tcgcttctgg gatctttgtt gtgttagttt ctggtgtctt
    27661 ttctaaaatt aaaaaactat tccctcctat agtaacagga acggttatca ccgttattgg
    27721 tttgactttg attcctgtag caatcgagaa aatgggtgga ggtaatgcaa cagcagctga
    27781 ttttggtgac aaaacaaatc tgctacttgc atttgtgacg attctcctaa ttgtaggcgt
    27841 gcaaatgctg gcaaaagggt tcattcgttc aatcgcggta ttgattggtt tagtcggagg
    27901 aagcatctta gctgctttca tgggaatgat tgatctgagc gcgattggag atgcgccgtt
    27961 gttccatgtt ccacaaccct tttattttgg taaaccaact ttcgacgtat ggtcgatttt
    28021 actaatgatc atcatttcca tggtcagcat ggtagaatca acaggtgtat attttgcact
    28081 tggtgacatt actgggaaaa atgttggaga agaagaattg aaaaagggat atcgtgcaga
    28141 aggacttgcc gttatcttag gcggtatttt caatacattc ccttatactg gcttttctca
    28201 aaatgtcgga ttggttcaac tttcgggaat caaaacaaga aaacccatct atttctctgc
    28261 attcttcttg attgttttag gattattccc taaaatcggt gcgttagcac aaatcatccc
    28321 tgagccggta cttggtggag gaatgttagt catgttcggg atggtggcag tccaaggaat
    28381 ccgtatgctg ttggaagttg atttcagcaa tgataaaaat ctattgattg ttgctgtttc
    28441 tgttggattt gggctgggtt tcaatgttac gccgacatta ttcagtcaaa tgccgcaaac
    28501 attgcaaatg ttcactggaa atggcatcgt aatgagtagc attacggcta tcatattgaa
    28561 tttgatcttt aatggattga aaaaagatga agaaatattg gaagaagcct tcaagaagta
    28621 atagagcaaa agaaaggttg tgaaatcagc gttttgacct gattgttaga gaaacaaacg
    28681 gaaaaatagt tcctcgtatt ttgccgtttg tttgagctaa taccgagggt cagctgattg
    28741 aacaccgttt atccataaaa aggaatgtga cctaactttt gtcacattcc tttttttcat
    28801 ctttaataaa cacgaacaat aaaatgtttt tctttgttaa cattcgaaat ttctttgaca
    28861 tcaaaaaaag tgattgttaa aatatgaatg tagaaaaact gggaggaaaa acaatgacag
    28921 caaaaatctc tgtgattatg gggagtactt cagattggga aacaatgaaa gaagcatgtc
    28981 aaatattgga tgaattcggt gtgtcttatg aaaaaaaggt cgtttctgct catcgaaccc
    29041 cagatttgat gtttgaatat gcacaaaacg ctcgaaaaaa aggaatcaaa atcattattg
    29101 caggagcagg gggagccgcg catctgccgg gtatggtcgc atcaaaaaca actttgccgg
    29161 ttatcggtgt accggtccaa tcacgaacat taaatggttt ggattcgctt ctttcgattg
    29221 tccaaatgcc tggaggagta cctgtagcta ctacagcaat cggaaaagcg ggagcaatta
    29281 atgctggact acttgcgata cagatgcttt ccatgtatga tttagaattg gaggctaaat
    29341 tggctgaaag acgttcaatg ttggctaaaa ctgttataga aagtagtgat caacttggtt
    29401 aaacctttgt tgcctggttc aacgatcggg atcgtcggtg gcggtcaatt aggtcgtatg
    29461 atggcgatta gtgcaaaaga aatgggcttt aaagtaggtg tgctggatcc tgtgagcgat
    29521 tgtccagccg cacaagttgc agattggcac attgaaagtg tctatgatga cacctttgcg
    29581 ctgaaagagc ttgccagaag aacagatgtc attacctatg aattcgaaaa cgtcagtgta
    29641 gaggcgttaa atgcgatttt acccatgtca tttattcctc aagggacaga cttgcttgcg
    29701 attacgcaag accgcctgtt agaaaaatct tttttggaaa cgaataacat cgtgattgct
    29761 ccttatgcaa cgatcgtcag tccgacggat atccaagatg cgattgacgg gatcggctat
    29821 ccttgtgttt tgaaaaccac tcgaggcggc tatgatggca aggggcagta tgttttgaaa
    29881 agtcgggcgg atctagctcc tgccatggac cttttaagag aaggaacctg tgttttagaa
    29941 gcatggatcc cgtttgaaaa agagatttcg atcatggtgg caggaaatgg tcaaggagat
    30001 ttcacgactt ttccggtcgt ggaaaacatc caccacaaca atattcttca tgaaacgatt
    30061 gctccagccg cgattgatca ggatgtgatc gaggaggctg agcgtattgc ccgtgtgatt
    30121 gcagaagcag tgagcctttc aggtgtttta ggggtagaaa tgtttttaac aaaaacaggc
    30181 gggctatatg tgaatgaatt agctcctcgt cctcacaatt ctggacatta ttctattgaa
    30241 gcttgttcta tgagtcagtt cgatgcccat attcgaggta tttgtggttg gccattagga
    30301 gaaaaagtac gtttgataag caatgcagta atggtcaatg ttttgggaga acaactgctg
    30361 gaaagctatc gcttgattga gaataagcca gactggcaat tccatttcta tggaaaaaaa
    30421 caagcaaaaa aaggtcgcaa gatgggacat atcactatct tgacagaaga catccagcaa
    30481 acgctggaag aaatcaaaca aacgaatatt tgggattaga ggagaaaaaa catgttagaa
    30541 cgttatacac gccctgaaat ggggaatatc tggacggaag agaacaaata ccgtgcgtgg
    30601 ctcgaagtcg agattttagc agatgaagct tgggcagaac ttggtgaaat cccgcaagaa
    30661 gatgtgaaaa aaattcgtga gcatgcatca tttgatatcg atcggatctt ggaaatcgaa
    30721 aaattaacac gccatgatgt cgttgcgttt acacgagcgg tttctgaaac attgggagaa
    30781 gaaagaaaat gggtccatta tggtctaaca agtacggatg tcgtagatac tgcttatggc
    30841 tatctcatca agcaagcaaa tgatatttta cgggaagact tgaaacgttt tacggaaatc
    30901 attggcgaaa aagcaaaaga acacaaatat acagtgatga tgggtcggac acacggtgtc
    30961 catgctgaac caactacttt tggattgaaa cttgccacat ggtattcaga aatgaaacgg
    31021 aacatcgaac gatttgaaca tgcggcaaaa ggagtagaag caggaaaaat ctcaggagca
    31081 gtgggaacgt tcgcaaatat cccgccattt attgaagagt atgtatgtaa acaattgggg
    31141 atccgtccac aagaaatctc tactcaagtt ctccctcggg atctacatgc tgaatatttc
    31201 tcagcgatgg cgcttattgc tacaagtatt gaacgttttg caacagagat acgcggattg
    31261 caaaaatccg aaacacgtga agtagaagaa ttttttgcga aaggtcaaaa aggttcttct
    31321 gctatgccgc acaaaaggaa tccgatcggt tcagaaaata tggctggtct agccagagtg
    31381 gtgcgcggac acatggtcac tgcctatgaa aatgtcacat tatggcatga aagagatatt
    31441 tctcattctt cagcagaacg aatcatcttg cctgatacca cgattttatt gaattacatg
    31501 ttaaatcgtt ttggcaatat cgttaaaaat ctaacggtct ttcctgaaaa catgaagcgg
    31561 aatatgtctg caacatttgg tttgatttat agccagcgcg ttatgctgaa attgatcgat
    31621 aagggaatga caagagaaga agcttatgat cttgttcagc caaaaactgc gtacgcttgg
    31681 gacaaccaag tgcagttccg cccgctgtta gaagaagatc caaccatcac atcggtatta
    31741 tctaaagaag aaatagacga tgcctttgat tatcatcatc atttgaaaaa cgttgacgtg
    31801 atattcaaac gagtaggttt ggaataaaga agagtgagtt gcaaaattca attaaaatag
    31861 aatagcaaaa ataaccaaaa aagaagcaga tccgttagcg gttctgcttc ttttagttta
    31921 ttatattttc ttatcaataa taggaacctg attttaaaag cggctacctc tttaccgtgc
    31981 ctctttacat acaaaaaata gaggaattaa gcgtaaatgg cttggctatt ataactaaaa
    32041 tgctggttat ggagatgttg cagcaagttt tcgagaaaag cgtattccat ctcttgtcca
    32101 ttgaataaga tctggatacg ataatgattt ttgaaacgct tctgttcatg aatctgactg
    32161 attgaaagtt cgcctttgtg ttctgtctga taaagaaaag tccttttacg gcattcagca
    32221 cagcgcttgg ttgctcggcg gatttttcgg aatgtataaa aactagtatc tttattgcat
    32281 aaataaacgt ctttgatcga cagcaaatca ttatatagac aggaacgttc ttcagctgtt
    32341 aattctgata aaaaaagctg accttgtttt attgcatctt ccatcggcaa gtgcctcctc
    32401 gaacccatta tccaaatttt tagttaattc taatatacca tcattaagta taaatgtaaa
    32461 cgcttgttga tggtataaga ttttcgtttc tgataaaaca cgaacattaa aagaatctaa
    32521 taccttaaat gtacatattt ttgttgattt ataaaaagat gattgctaaa atttaaagtg
    32581 aaaaaacgaa caaaaaacga tgtatttgct tttaatgtaa ataaaaagga gagacgtaca
    32641 tggagaagaa agaacttttg tatgaaggaa aagccaaaaa aatatatctt acttctgatg
    32701 agaacttgat ttggatagaa tatttagatc aagcaactgc gttgaatggc gcgaggaaag
    32761 accagataag tggaaaagga gaattgaata atcaaatcac aagtgcaatt ttccgttatc
    32821 tgactcaaag aggaataaaa aatcatttta ttgaacaatt gtctaaaacc gaacaattag
    32881 caaaaaaagt acaaattatc cctctggaag tggttatccg aaatattgca gcaggaagtt
    32941 tctcaaaacg tctaggtgtt cccgaaggta aagaattcat caaaccaatc attgaattct
    33001 actataagga tgaccagttg gatgatccat ttatcaatga agagcatatc tatttcttac
    33061 aacttgcaaa accggaagaa atcagtgagc tgaaagccca agcgttggct attaatgaaa
    33121 ttttgaccga attatttgct aaggtcggtt tggttttagt agatttcaag ctagaatttg
    33181 gccggtcgaa tggacaaatt ttgcttgcgg atgaagtaac accggatact tgccgattat
    33241 gggatgaacg tacgaatgaa catatggaca aagacgttta ccgcagagat ctaggtgatt
    33301 tgatcccagt ctatcaagaa gtatataacc gtctatcgga actaactgaa gaaaaggagc
    33361 gagtatgatg tatttcgtaa aagtatatgt aacctataaa gattcagtgt tagatcctca
    33421 aggggaggca gtaaaaaaag cagtccatcg tatgggatat gaaacaatcg aagatatccg
    33481 aataggaaaa tactttgaga tacaacttgc tgatgcagaa gaaccaatcg atacagtgat
    33541 tgaaacgatt tgtgacaagc tgcttgcaaa tgtaaatatg gaaacatacc gatatgaaat
    33601 caaaaaaatg gaggaagtat aacatgaaat ttgcggtcat cgtttttcca ggttcaaatt
    33661 gtgacgcaga tatgttatgg gcagttcgcg agatcatggg cgctgatgcc gaatttgtac
    33721 ggcatgatga gtcctcttta gaaggatttg atggggtttt actgccggga ggattctcct
    33781 atggtgacta tttgagatgc ggtgcgattg ctcggttttc tacaatcatg gaagaagtga
    33841 ttcgttttgc tgaagaaggg aaaccagtat tcggtacatg caatggattc cagatattga
    33901 cagaagcagg acttttgcca ggtgtactga gacgaaatga gtctttgcat tttatttgca
    33961 aaactgtacc attgaaagtc aaccccaaga cggcctttac ttcagagtat gaagccgacg
    34021 aaataatcta tcttccagtt gctcatgggg aaggaaatta tttctgtgac gaagaaacac
    34081 ttcaagaact aaaaaaaaat aatcagattg cctttactta taatgacaac atcaacggca
    34141 gcgtggaaaa cattgcaggg attacgaaca aagcagggaa tgtgttagga atgatgcctc
    34201 atccagaacg tgcagtagaa gacttgctag gatcagaaga tgggaaacgg ttctttgctt
    34261 ccattttaaa aaacttcgga aaggtgaccg tatagatgaa aacattgaaa gaaccgacag
    34321 ctgaagagat caagaaaaaa agaatctatg ccgaatgggg attgactgat gaagaatatc
    34381 gaatgatcga agaagatatc ttaggaagaa tgcctaatta tacggaaact ggactgtttt
    34441 ctgttatgtg gagcgaacat tgttcctata aaaattctaa accggttcta cgcaaatttc
    34501 ctactagtgg acctcatgtg ttgcaaggcc ctggagaagg cgcaggcatc gtcgacatcg
    34561 gtgatggact agcagttgtc ttcaaagcag aaagtcacaa tcatccttca gccgtggagc
    34621 cttatgaagg agctgcgaca ggagttggcg gaatcatcag agatattttc agcatggggg
    34681 ctcgtccaat tgcgttgcta gattcattga ggttcggcga attagataat ccaagaacga
    34741 aatatttact agaagaagta gttgcaggta tcggcggata cggcaattgt atcggcatac
    34801 cgactgtagg cggcgaaatt gcatttgatc cttgttatga aggaaaccca ttagtcaatg
    34861 caatgtgcgt aggtttgatt gaacacaaac atatccagaa agggcaagcc gctggcgtcg
    34921 gtaattcgat catgtatgta ggtgcaaaaa caggtcgcga cggtatccac ggagcaactt
    34981 ttgcctcaga agaatttaat caagaagaag aacagcaacg ttctgctgta caagtaggcg
    35041 atccttttat ggaaaagctg ctgcttgaag cgtgtttgga attaatcttg catcattcgg
    35101 attctctaat aggtatacag gatatgggag cagcaggact tgtttcatct agtgcggaaa
    35161 tggcttcaaa agctggctca ggacttatat tgacattaga tgaagtccca caaagagaaa
    35221 ctgggatgac accttatgaa atgatgttat cggaatcaca agaacggatg ttgatttgtg
    35281 ttaaatcagg agaggaagaa aaaatccaag aactatttca gaagtatgat ctagatgcag
    35341 tcactatcgg taaagtaaca gacgacgggc aatatcggct atatcatcaa ggagaagaag
    35401 tagcaaatct gccagtcgat gcattagctg aagatgcacc agtttatcat aaagaaatga
    35461 aagaaccagc tcgaatcgta gaatttcagc agttagctcc ttatcagcca gttatcgaag
    35521 aacctggaca agtattgctt gatctgctgc agcaaccgac gattgcttca aaacgttcaa
    35581 tctacgaaac ttatgattca caagtacaaa ccaatacagt tgtccgtcca ggaagcgatg
    35641 ctgctgtgat gagggttaga gggacgaata aagcgttagc tatgaccaca gactgcaacg
    35701 cgcgctatct gtatttggat ccaaaaatcg gaggacaaat agcagtggca gaagctgcta
    35761 gaaatattat tgctagcggt ggtaagccct tggcgatcac cgactgcctg aactatggtt
    35821 ctcctgataa accagaagtt ttttgggaat tatcgacttc agctgacgga attgctgctg
    35881 cgtgtgaaac gttaggaact cctgttattt caggaaatgt ctctttatat aatgaaacgg
    35941 atggtcaagc aatctatcca acaccaatga ttggtatggt cggattgatt gaggatcata
    36001 aacatatcac tactcaggaa ttcaaaaaat caggggatct aatctatata ttaggtaaga
    36061 cgtttgcaga ttttgatggc agtgaattac aaaagatgca gcttggaaga atcgaaggtg
    36121 tgatccgaaa ctttgatcta tccattgaaa agagaaacca agagttagta ttgacagcca
    36181 tccaaaacgg attgattgaa agtgctcatg actgttcaga aggtggcttg gcaattgcac
    36241 tggcagaatc agctttcaaa catcagctag ggatatctgt acagtttgaa ttgtcctctg
    36301 ctcaattatt tgcagaaact caatcaagat ttgtgttgac agtcgcaccg gaaaataaga
    36361 cacgattcga agaaatgatg ggagatgctg ctgttttagc aggtaaggtg acggatgaag
    36421 cgataatcga aatttcagca actgacggtc agatcaaaat agaaactgca gtagcaagaa
    36481 agtgctggga ggatgcaatc gtatgtctta tgaagtgaag agtttaaatg aagaatgcgg
    36541 gatcttcggt gtttggggtc atccagacgc cgctagtgtg gcatacttcg ggctccatag
    36601 tcttcagcat cgaggacaag aaggtgcagg tatcgtgtcc aatgatcgag gaaagcttaa
    36661 ggggcatcga gatttggggc tgctttctga ggtattcaaa gatcaaagaa aattgcagca
    36721 gttaacagga gaagctgcaa tcggacatgt acgttatgca acagcaggca atggaagtgt
    36781 ggacaatatt cagccttttc tattcaaatt tcatgatcag tcgatcggct tagcgcataa
    36841 tggtaacttg atcaatgctc gttcccttag aaaagagtta gaggaaaatg gggcgatctt
    36901 ccattctaac tccgatacgg agatattgat gcatttgatc cgacgaagtg aagaaacaga
    36961 ttttttaaat aaattaaaaa gtgctttgaa tgaagtcaaa ggtggctttg cctatttatt
    37021 aatgacagaa acggcaatga ttgccgcttt ggacccaaat ggttttcgtc cattagcaat
    37081 cggacaaatg aaaaacggag catacgtgat tgcctcggaa acttgtgcat tagaagttat
    37141 tggggcacgt tttataagag atgtccagcc tggtgagata gtgatcatca atgacgaagg
    37201 aatccagatt gattgcttta cgaaggacac gcagctttct atttgttcga tggaatatat
    37261 ctattttgcc agaccagatt caaacatcgc agggatcaat gtgcatactg cacggaaaaa
    37321 tatgggacgg aatttagcga aagaatcgcc agtaaaagca gatatggtcg ttggcgtacc
    37381 taattcttct ttgtcagccg caagcggtta tgcggaagct agtggaatcc cttatgaaat
    37441 cggattagta aaaaatcaat acattgcccg aacatttatc cagccaacgc ctgagctaag
    37501 agagcaaggt gttcgaatga aattatctgc tgtacgagga gtagtggaag gtaaaaaggt
    37561 gattatggtc gatgattcaa ttgttcgggg tacgacaagc agacggatcg tccatctttt
    37621 gaaagaagca ggggcaaaag aagtccatgt gaggattgct tctcctcctt tgaaatatcc
    37681 atgtttttac gggattgata ttcaaacgcg taaagaactc attgcggcaa atcacacgat
    37741 tgatgaaatc aaagaatgta tcggtgcaga ttctttaggt tttttaagtg aagagggtct
    37801 gatcgaatcg atcggtctgg aaaaagatgc tccgtattca ggattgtgta tggcttattt
    37861 caacggagat tatccgacgc cgttatatga ctatgaagaa aaatatcagg aatcactaaa
    37921 agaaaaagta tcgttctttt aaaagcagac caagaaggga gtatatgaat tcaatggcaa
    37981 atgcatacgc aaaagctggc gtagatgtcg aagctggata tgaagtagta gaacggatca
    38041 agaagcatgt caaaaaaacg gaacgtttag gtatgatggg cgctatagga ggattcggcg
    38101 gttgttttga tttatcggtc tttcaattaa aagagccagt ccttgtctcg gggaccgatg
    38161 gcgtggggac gaaattagca gtggcaatcg aagcagacaa acacgataca atcgggatcg
    38221 actgcgtagc gatgtgtgtc aatgatatct tagcacaagg cgcagagccg ttatatttct
    38281 tagactactt agcaacgggg aaaaatgatc cgttacgttt ggaaaatgta gtaaaaggtg
    38341 tagcagatgg atgtgttcaa ggaaatatgg cgctgattgg cggagaaaca gctgaaatgc
    38401 ctggtatgta ctcaggtaat gattatgatc tagccggatt tgctgtaggg atagctgaaa
    38461 aatctcaatt attgacagga gaaacgatca acccaggaga tgtgttgctt ggaatagcat
    38521 ctagcgggat tcattcaaat gggtactctc ttgttcgcaa aattttcttt gagcagcagc
    38581 agttttcttt atcatcaaaa ctgccagaat tgaaaaaaac acttggcgaa gaattgcttg
    38641 agccaacgaa aatctatgtt tcttccttgc ttccgctgat caaagaaaag aaaataaaag
    38701 gagctgcaca tatcactggt ggagggtttg tagaaaatat cccgcgaatg ttaccagaat
    38761 ctatggctgc acaaatcaaa cttggctcat ggccgattct tccaatattc ggctgtttac
    38821 aaaaatatgg tgaaattcct gagatggaga tgtatgaaat cttcaatatg ggcatcggta
    38881 tggtcctggc agtcgaagaa tcgaaagtaa aagaaatagt acacgaaatt gagcaagctg
    38941 gcgaaaaagt ttatgaaatc gggaaagtga tcgccaaaga aaaacatcgt gtggtgttta
    39001 taggagaaga acgatgagaa tcgctgtttt tgcttctgga aacggaagca actttcaagc
    39061 gctagccgat tatctatcga aaaaaggatt ggaatcttcg attgactggt tgttttgtga
    39121 tcagcctgaa gcatatgttt tgaaacgtgc aactgcgttg agtgtaccgg cagattgttt
    39181 ttcacctaaa gaatttgatt caaaaaaaga gtatgaagaa gcaattttgc ataaattgaa
    39241 agaaaaaaag atcgatttga tcgtattagc tggatatatg cgaatcattg gaccggtatt
    39301 gctgaaaaat tatgataaaa gaatcatcaa tatccatccg tctcttttac ctgccttccc
    39361 tggtttgcat ggcatacgtg atgcctttga agctggagta aaagaaacgg gtgtcacaat
    39421 tcattatatc gaccaaggtg tagatactgg accaatcatc cggcaggaaa aagtgagaat
    39481 tgaacaggag gacacgttcg attctttaga agaaaaaatc catagagtag agcatcgaat
    39541 ctatccagaa gtgattagtg aaattattga aaatggaggc tatctgaaat gacaagagca
    39601 ttgatcagtg tatcagataa aactgggatt gtagcattcg cccaaggttt gattgaacaa
    39661 ggagtagaaa tcatttcaac aggaggaaca aagcatgcgt tggataaagc tgggatcaaa
    39721 acgctttcga tcgaagaagt gacagggttt cctgaaatga tggatggccg tgtcaaaaca
    39781 cttcatccta aaatccatgg tggtctttta ggaagacgcg atctccctag ccatatggag
    39841 gcaatggaaa cggaaaatat ccaaccaatc gactttgttt gtgtcaattt atatcctttc
    39901 aaagaaacga ttatgaaacc agatgtcgaa atagcagaag ctattgaaaa cattgatatc
    39961 ggtggtccta gtatgctccg tagtgcagca aaaaaccatg aatttgtcac ctctgttgtt
    40021 gatccatctg actatgaaga agtactttca gaattacgtc agtatgggca tacttcactt
    40081 tctttcagac aaaaactagc ggcaaaagtc tttcgtcata ctgcggcata tgatgcactg
    40141 atcgctcaat acttaacgga ttgggtggaa gaaaaagaac cggaaaaatt gacacttacg
    40201 tatgaacgaa aacaaacgct tcgttacggt gaaaatagcc atcaagccgc tgccttttat
    40261 cagtccgctc ttctggaaaa atattcgatc gcagcagcaa aacaactcca tgggaaagaa
    40321 ctttcttata ataatatccg agatgcagat gctgcgctac ggatcatgaa ggaatatgac
    40381 gagccaacgg tagtcgcctt gaaacacatg aatccttgtg gtattgggac agcatctacg
    40441 attttagaag cgtgggaagc agcgtatgaa gcagatcctg tttcaatctt tggtgggatc
    40501 atcgttttga atcgagaagt agatctgcca acagcagaac agatgcataa gctgtttcta
    40561 gaaatcatca ttgccccttc ttatacacaa gaagctttgg aattactgca aaaaaagaaa
    40621 aacatccgat tactgacagt agactttgcg caggaagaaa atcaaaatcg agaagaaacg
    40681 gtctcagtct taggtggact tcttgtacaa gatcaagatc tggcaataga aaatgaagaa
    40741 gagtggaaag tcgtgacaaa acgtaagccg actccagcag aagcaaaagc attagcgttt
    40801 gcttggagag ctgtcaaaca tgtgaaatct aatgcgatcg tactggcaaa cgaacgtcag
    40861 acagtaggaa tcggagccgg gcagatgaat cgagtcggtt cagtaaaaat tgcgattgaa
    40921 caagcgcaag agaaaatgga aggtgcagtt ttagctagcg atgcttattt tccaatggat
    40981 gattcggttg aatatgcagc caaacatggt ataaaagcaa tcattcagcc aggaggaagc
    41041 atcaaagacc aagattctat tgatatggcg aataaatacg atctagcaat ggtctttact
    41101 gatgttcggc attttagaca ttagagagga gtcggataaa tgaacatttt ggtgatcggt
    41161 gcaggtggtc gtgaacatgc aataagtaaa aaacttttag ccagtccgaa ggtggatacg
    41221 gtgtattgtg cgccaggaaa tccggggatg acaaaagatg gtatcaattg tgtcgctatc
    41281 agtgagcgag atcattcagc gttaatccat tttgttaaga aagaggctat tgattggagt
    41341 atcgttggtc ctgaagttcc tttgttaaat gggatcgtcg atgattttat ggcggaagga
    41401 ttgaaaatct tcggtccgac aaaagcagca gctctgatcg aaggttcgaa aagttttgct
    41461 aaacaattaa tggatactta tcaaatcccg acagctaaat ccagaagttt tgaatgttac
    41521 gaaaaagcag cagcgtatat caaagaaaat ggtgcgccaa tcgtagtgaa agcggacggc
    41581 ttagcagctg ggaaaggtgt agtcgtggct accacagtga aggaagcgct ggatgcggca
    41641 gaacaaatgt tgaaccagca tcgatttggg gatagtagca aaaaaatcgt cgtagaagag
    41701 tttttggcag gagaagaatt ctcccttttg gcgtttgtcc gtaataccaa ggtgtatccg
    41761 atggtcattt cgcaagatca taagcgggca tttgataacg ataaagggcc aaatacaggc
    41821 ggaatgggtg cttattcacc agtaccgcaa atttcaaaaa agatgattga aaaagctgtg
    41881 gaagaagttt taaaaccagc ggctttggga atgaaagaac taggacgctc tttcacgggt
    41941 gttttatatg cgggtttgat tgctacgaag gaaggaccaa aagtgatcga attcaatgcg
    42001 cgtttcggtg atcctgaaac gcaagtcgtt ctttctcggt tgaagagtga ctttgcgcaa
    42061 gtaatcgatg atttattaga gaatcgtaca gtagagttaa agtggcagca agaaggatac
    42121 aatgtaggcg ttgtggttgc agctgccggt taccccgaag aatatgagac aggaatggtt
    42181 ctaccagatt ttctggaaga agagctttct gtttactatg ctggagtttc ctcaaataac
    42241 ggcaaactag ttggagcagg aggacgtctt tatttagtag aagcatacgg tgagacgatc
    42301 aaagaagcac agaaaaaaat ttacaccgcg ttaatggata aaacgacaga tggaacattt
    42361 tatcgtacag atattggaga aaaagctgcc aatagcgaac tgatcgtgaa gtaaatcagc
    42421 tgcttccatt tataaaaaat acaacaaaca taatcggaag cgccgatttt agtgaagcct
    42481 tttcataaag agtgtggtac acttttgttg tgtatataaa taaggaggag ttcagatgaa
    42541 aaaaattcaa ttaggtactt ctgatcagaa agcagctgca gtgatcttag gctgtatgcg
    42601 tatcaacaat gcggaagatc cagtaaaagt gattgagaca gcttatgaaa acggcattga
    42661 tttttttgac catgcagata tttatggaaa cggtgaatgt gaagaaatct ttgcagatgc
    42721 tctagcaaaa acatcaatca aacgagaaga tctttttatt caaacaaaat gtggaatcgt
    42781 tccaggtgtg atgtttgatt tttctaaaaa acatatcatt gaatctgtag aaaaaagttt
    42841 gacccgtctg aaaatggatt atgtggatgc tttacttctt catcgtccag atacattggt
    42901 agaaccagag gaagtagctg aagcgtttga tgaattggaa cggtcaggaa aagttcgtta
    42961 ttttggcgtc agcaatcaaa aaccaatgca aatcgaattg ctgaaaaaat atgttcgtca
    43021 accattagta gctaatcagc tgcaatttgg cttgaaacat actggaatga tcgatcaagg
    43081 gattcatgtc aatatgacag atgaagcaag tatcgatcat gacggtagtg tacttgatta
    43141 ttcccgattg catgatatga cgatccaagc atggtcacca taccagtatg gattttttga
    43201 aggtgtattt atcgggaacg aaaaattccc tgaattaaac caaacattag aagaactagc
    43261 agctaaatat caaacgacac caactggctt ggcttctgct tggattttac gccatccagc
    43321 caatatgcaa gtgatcgccg gtacgatgaa cgtagggcga atcaaggaaa tcgctcaagc
    43381 ttcagaaatt aatatgtctc gggaagattg gtacgaattg taccgtgctg ctggaaacat
    43441 tttaccttaa agattgtcat aagcaggtca aaagggctat aaattaaaaa gacagaataa
    43501 cttgaaagct gttttcagct tcgagatatt ctgtcttttt ttattgcaaa tggaaagagt
    43561 atcattgtac ggatttcgtt tattagttat ttttgttttt aaggaaaagc tgtgtccgat
    43621 attcttttgg agaaatccct tgttttttct taaataaata actgaaatag ttagggtcat
    43681 tataaccaat ttcaaaagca atatctgaaa tacgatagtc tgattgctgc aataattttt
    43741 ttgctaattc gattctttgt tctgtcagat aatcgatgaa tgtttggtta aacgattgag
    43801 aaaaaatcgt actgaaatga gagggactta atgcaacttg ttgagcgacc caagttaagg
    43861 acatatctgg atcggtaaaa ttttctttga taaaatccaa tgcatggttc aaaattggct
    43921 gatatttgtt ccttgaaggc tgtgagggca gagcagttaa gaaagaaatg aacatataaa
    43981 ctgtcgaacg gtaaagcgag tcgtcggcgc tgattgctaa gcgttgactg gtggatgtgc
    44041 aatgaaccat ttcttgcggg attttctttt ctttcaggca gacatgctgg agtagcttag
    44101 tcaagacaaa taaacgatat aatcgtgttc gagttggtgt gccttgtggt ttcgtcaagt
    44161 gttcgatgta cttagatact tcgtttttcg ataatgaaga caaatcaagt gagaggtcaa
    44221 agtaaaagac agaatcaact ggaagtttcg cttcttgaat caaatcctca tagctgataa
    44281 tttgttcttt aagggaactg gcggtttcaa gtatcatctg cgtgatctca aaagatgcag
    44341 gtaattcgct gattctttct gcgacaggac caatggcgac agaaaaatta tttttttgtc
    44401 ccttttctaa ttcatggacc aacgtttggg ctacctgata actggtctcg atcacatttt
    44461 cttcaagaga atcaactatt aaaaatttga tgaatcgaga ggaaacagca gagaagatca
    44521 cttgttgatt ctccctaaat aaaaacttca aataatcact gagatgagcg taatcttcaa
    44581 agtctgtact gacttggttt gctgctaata atacggtcat ttttttccct gctaaagagc
    44641 gattgaaaga ttcagcaagt ttcaatgttt cactgatcgt aagtgagcct ttgaacaaac
    44701 cgttcaagaa atgatttttt ttcaattcga aaacaagatc agaagaagtg tgcgtttctt
    44761 tttgctgcaa atctaaagca gttactgctt tttctattgc ttgtttcaat tcgctacttt
    44821 taatcggttt aagcagataa tcatctgcct gtacctggat tgctcctttc atataatcga
    44881 aatcgtcgaa tccactaata aatatgagac gagtccaagg aagtagtttc cttgcttctt
    44941 ttgaaaaagt caacccgtcc atgaaaggca tttggatatc agtcagaatg atgtcgggct
    45001 gtaagtcgat aattgaagac agtgccatct caccatctcc agcttcgccg acataagtga
    45061 ttggataata agaaccaagg acagttattt gttcccgcaa gttattgcga atgaggtatt
    45121 cgtcttcgat gataaaaact ttatacatgt ttgtttcctc caattgtttt gggagataga
    45181 gggatcatca gtgtgacagt tgttccttct cggtatttgc tgtcaatcgt gataggtttt
    45241 tgatcatttc cgtaataaag aaggagacgc ttgtttacgt tgtaaagtcc gtagccgata
    45301 ctgaaatctg aatcgatccc tttggctaac tctttacgta tttctgccag tttttctgca
    45361 gggatgccaa tcccgttgtc tataactttg aattggatca tctcatcaat tttgcatgcg
    45421 gtaatacgaa taaatccttt gtgacggacg tacttcgttc catggtatac agcattttca
    45481 atcaatggct gcaagatcat ttttaaaata gaaacaggaa aaagtgtttc tggtatatcg
    45541 atcttatagt caagcatatg gccgtatctt atttttaaga tgactagata atcattgata
    45601 tggctaattt ctttttcaag aggaatccag tcttgtcctt tgcttaaaga cgtacgaaaa
    45661 aagccggaaa gtgcatgggt catagtcaga gcttcttcaa agcagtcttg ttcgatcaaa
    45721 gaaataatgg catccaaact gttataaata aaatggggag taatctgagc ttgcaataca
    45781 cgaacttcac tttgtgctaa atggtattgt ttcaaagcgt tctcccgtaa agactggttt
    45841 agatcttccg ccattttatt catgctagcg gtaagtgttt gtaattccag tgtttccggc
    45901 agggttaaac gaaagccaag gttccctgaa gaaagttgtt ctgacatctc gatcagctga
    45961 ttgatcggtt cttggacctt tctctctaaa aagcgacgat tctttttcgc atagaaaaaa
    46021 atggtactaa gaactccaag ttccactatt gttaatagaa tgagcgagcg gacaagtgtc
    46081 gtattttttt tgcttgctaa gtcgatctcc acacgaacaa aatcttgtaa gatatctgac
    46141 agcagttgag tgacagaatc tacctgattg atgatctctt cattggtatc cataaaatct
    46201 tttgccaaga tattattgat cagctcgttt tgataatctt ccagtgtatc gatcgtacgt
    46261 agcgatacat ctaacgtact aatttcttta agtgtatttg tattattttt gatctctcga
    46321 atatcattcc taagttcttc taggatattt tcctcattga actcatctac ggttacttgg
    46381 ccagaaatca agtcccacat actttccaaa actttttgat caaccttcaa agcaattgca
    46441 tctgcttttt ggatatttgc gatcgtctgt tgataaatca aaagctgttg cgaatataac
    46501 agtatgctca gtagcaaagg gataacagca aatagaagca tggctttgtt tgcttgggca
    46561 aaaatctgtt ttatgcttaa attttttgtc atgatacttc tccagtcaat aatttctggt
    46621 tgggatgtta gctatattct gctgggaaaa gacagtctcg gaaataaaaa tatctttagg
    46681 taatgttttt cctgtttgaa tatttccaaa ataacgagta acggtattgg ctacatacca
    46741 gccgacatca ggtttacact cgactacaca attgacgatt cctgagcgta gcttctcgat
    46801 catatcttct tgtccatcaa tagttacaat gatcaaatct tctccaggtt tgattgatgt
    46861 ttcttttatc gcagctaaag caccgtgagt catttcatca ctatggctgt ataatacatc
    46921 gatttcggat aaatcatttg tttctaaata tcgtttcatc tggtcttttc ctttcatccg
    46981 aataaaatca ccgacaattc ggtgaacaat agtcatcgga ggtttatggt cgatggttcc
    47041 atcttttttt agggttggtt tacgattgat tgattccaaa aatccttcgg agcgtagctt
    47101 ggtcggtgtg gagttctctg aaccagcaag ttctaggatt ctgatttcct gtttctttga
    47161 atttgtgaag tgattgctga caaataaacc cgcccgattt ccttctgctt tgaaactggg
    47221 gccaatgtgg gtgacgaaaa gctcgggatc attcgtttga atatgtctgt cgacaagtat
    47281 gacagggatc cctgctcgct ttgcttcttt taatacagag tcccacccgg attcttgcaa
    47341 aggagtaaaa atgatcatat ccactttata ggcaatgaac gtccgtatat cttggatttg
    47401 ccgttcctga ttcatgtagc catttcggta gagaacttgg taatcttctt tttcaagcgc
    47461 agtgataatt gattcagtat gctttttccg ccagctactc tccgttcctg attgcgaaaa
    47521 accaacgact agtgggtcag gtgcttcctt tgtattaggt gtacacccca tcatttggta
    47581 agcgattaca acaagggcta aaaaaaataa aacacgtttc actttaaatc actccatctg
    47641 gatttgttag tactatctta tcagataaaa gggaaaatca aagtcaaaag gaatcaatcg
    47701 aaaaaaatcc aagaaactta aaaaaatcca agacaaaaaa agtacaccta aaaaatttaa
    47761 agtaaatccc ataaaaaaat caatgttaac gctttcttta ttagctatac tcggtgtatc
    47821 aattgaaatg ggggataaaa aatgaaaaac aaatttagaa agattgttgg gactatcgct
    47881 attttgaccg caagcattct attaagtgcg tgtggcaatt caggatctgc agatgatagt
    47941 acggggtatg taggaatcgc tatgccgacc aaatcagcag aaagatggat cgctgatggg
    48001 aacaacatgg tctcggaatt agaaaaactg ggttataaaa cagatcttca atatggagaa
    48061 gataaagtag aaaatcaagt agcacaaata gaaaatatga ttacaaaagg ggtagataca
    48121 cttgtgattg cttctatcga tggttcggct ttaactgatg tgttagctaa agcaaaagaa
    48181 gcagatatca aggtgatcgc ctatgatcgt ttactgatga attcagaaaa tgtcgattat
    48241 tacgcgactt ttgataattt tggagtaggt gtatcgcaag cagcttatat tgaagagcac
    48301 ttaggattga aagaaggaaa aggaccattc acgattgaat tatttggtgg ctcgcctgac
    48361 gacaacaacg cactaatcaa ttacaacgga gtgatgtctg ttttacaacc atatatggac
    48421 aacggtcagt tggtcgtgcc atctggacag acgagtttca gtcaaatcgc tacgcttcgc
    48481 tgggatggtt ctacggcgca agcaaggatg gataatctgt taagcgcaaa ttatactgac
    48541 caaacattgg atgctgtttt gtcgccatat gatccaataa gtttaggaat catttcgtca
    48601 ttaaaaggcg tggggtatgg gagtgaaagt aaacctttac ctgtgattac tggacaagat
    48661 gcgacagttg ctggcgttaa atcgatcatt gccggggaac aaacacaaac gatctttaaa
    48721 gacacacgta tcttagcaaa aaatacgatt gaaatgatca aagcaatcag tgatggagaa
    48781 gaggttccgg tcaatgatac tgaaacttat gataatgggg tgaaaactgt ccctacgtat
    48841 ttagcaaata cagtatctgt cgataaagat aattatcaag cagagctgat cgacaccgat
    48901 tattataaag aatccgatct gaaaaattaa gaggaaccaa acaaggaact aaagagatct
    48961 aagtcagaga gaggataaga attatggcgg attgtatttt aaaaatggca gatatcacca
    49021 aaaaattttc tggaatccga gcgttagatt gcgtgaatct gatgattgaa cgtggggaaa
    49081 tccatgcatt gtgcggagaa aacggagcgg gaaaatcaac attgatgaat gtcttgagcg
    49141 gcttgtatcc atatggctcc tatgacgggg aaatctttta tgatggaaag ctgtgtcaat
    49201 ttaaagattt gaaagatagt gaagaaaaag gcatcgtcat cattcatcaa gaattagcac
    49261 taagtcctta tctttcaata aaagagaata tttttctcgg aaatgaacaa gcagaaagag
    49321 gcgtgattga ttgggaactg acagaaaaga aaacatatga actgctgaat aaagtcggtt
    49381 tgtctatcga cccgaatctt ctagtatccc agattggcgt agggcaacag caactagttg
    49441 aaatagctaa agcattatca aaatccgtcc gtctgttgat tttggatgag cctaccgctg
    49501 cattgaatga agaagaaagc gcgaatttgc tgagattgat ccgagagttg aaagagcaag
    49561 gtgtaacctc catcattatt tcacataagc tgaatgaaat cgtggatatc gcagatcgaa
    49621 tcaccatttt acgggatgga cagacgattg agacactaga aaatgaaatg atcagcgaag
    49681 agcatattat tcgcgggatg gtgggcagag atttagttga tcgttatcct gaaagaaaac
    49741 cggatattgg agaagtttat tttaaagtag aagactggac ggtctatcac cctcttgatc
    49801 acgagcgaat cataaacgac cacatcaact tgcatttgaa aaaaggagag attgtaggaa
    49861 tagctggact gatgggagcg ggcagaactg aattcgctat gagtctattt ggacattctt
    49921 atggaagcaa gatatccgga cgtatattaa aggaaggggc agagatatca gttaagaccg
    49981 ttccttcagc cattgcaaac ggactcgctt atgtctcgga ggaccgaaaa tctttaggac
    50041 tgaatctgct gatggatatt cgggaaaacg tgacgctgag cagtttggat aaaataagtc
    50101 aaaaaggagt attagataaa gaaaaagaag taactgaggc agaaaatttc cggaaaaaga
    50161 tgcgtataaa aacaaactcc atccatcaaa ttgtcagtag tcttagtggt gggaatcaac
    50221 aaaaagtcgt tttatccaag tggctgatga cacaacccga tattcttttt ttggatgagc
    50281 caacccgtgg gatagatgtt ggggcaaaat atgagatcta cacgattatc gaagaaatgg
    50341 cagcaaatgg caaagctgtc tgtcttatct cttctgaact tcctgaaata ttagggatgt
    50401 gtgatcgaat ctatacgatg aacgaaggac gtattacagg agaagtctcg cgagaagatg
    50461 cagatcagga agtcttgatg aaattaatga cgaaagaaga ggtgggttaa gttggaacag
    50521 gcaaaaaatc ttacggataa aaaagagaaa aaactaaatt tgaaaagtag agcagctgat
    50581 ctttttggtc gctacagcat ggtgatcatt ttagcgggac tacttattgc gtttcaattg
    50641 atgacggatg gtattttctg gcgtccattg aatataacta accttgttct gcaaaatagc
    50701 catatattag tactagctgc agggatgtta ttagttgtgc tcttaggtca tgtggatttg
    50761 tcggtagggt cagtgatggc gtttgttggt gcaattgcgg gcatgatgat ggtcaatcgc
    50821 caaatgaacc cgtggatgac agttatcgca tgtcttgcag taggcgggct gatcggcgca
    50881 tggcaaggtt tttgggtagc ttatgcaggg ataccggcgt ttatcgtgac tttagcagga
    50941 ttgctgatgt ttcgtggact gacacaagtt gttctaggag gccaatctct agcacctttt
    51001 ccagctgttt tccaaaaaat gtctacgggt tatttaccag acttaagcga aggaagcagc
    51061 cttcacttct tgacattgat tttaggaatg atcatcacga tcgtgttagt tgttggtcaa
    51121 tggcgaacaa gaaatcgtcg gaaaaatcat ttatttgaag tggaatctcc aacagctttc
    51181 ttggtaaagg cggtatttac agcagtcgtt gtactaggaa taacttatat ctttgcctct
    51241 tatcaagggt atccgattat tttgatcatt cttggtttga tcgttgcagc atatgggttt
    51301 ttgacaaata aaacagtagc aggtcgacag atttatgcga cgggcggaaa tcgaaaagcg
    51361 gctgaactat cagggatcaa aacaaagcga atcacgttct gggtattcgt caacatggga
    51421 gtcatggcag ctttagctgg attagtttta gctgctcgat tgaatgcggc aacacctcaa
    51481 gcaggtacat cgatggaact ggatgcaatg gcggcagtat attttggtgg tgcttcgact
    51541 tccggcggca ttggaacaat catgggtgcg attgtcggtg gcttggtcat gggtgtattg
    51601 aacaatggca tgtcgatttt aggcgtcggg gtagactggc agcaggcaat caaaggattg
    51661 atcttgcttt tagccgttgt gctagacttg tataataaga aaaaacgaac aacgtaggag
    51721 aacgccggtt attcagaaaa aagactgtga caagacattt gtcacagtct ttttttcgct
    51781 aaaagcgaac attcattgtt tcgtttgtat agactaaaaa taatatatgt ttttttacca
    51841 ttcgtataaa ttctcttttt gctcttgatt tttttgctta attgagttaa attacatagg
    51901 gcgctaaaaa aagccttgat taaattaata atgttcgtgt tttgctggat gtgaacatca
    51961 ggaggataat gtaatgaaag tatttgatta cgaagatatt caattaattc ctaataaatg
    52021 tatcgtaaat agccgttctg aatgtgatac aacggtcaca ttaggtaaac atacattcaa
    52081 aatgccagta gtgcctgcca acatgcagac gattattgat gaaacaatcg cagaattctt
    52141 agcggaaaat ggttatttct atatcatgca tcggtttgat gaagcagctc gaattccgtt
    52201 tatcaaaaaa atgaaaaagc gtggattgat tacctcgatc agtgttggcg tcaagaaaga
    52261 ggaatattcc tttatagaga agttggcaga agaatcgcta aatcctgatt atatcactat
    52321 cgacatcgcg catggacatg ctaactcagt tattgatatg attcagcata ttaaaaaata
    52381 tttgcctgaa acttttgtga ttgccggtaa tgttggtaca ccggaagccg tccgtgaatt
    52441 ggaaaatgcg ggcgcagatg caacaaaagt cgggattggt ccaggaaaag tatgtattac
    52501 aaaaatcaag acaggctttg ggactggagg ctggcaactg gcagctctta gatggtgtgc
    52561 gaaagcagct cgcaagccga tcatcgctga cggtgggatc agaacacatg gcgatattgc
    52621 aaaatctgtc cgttttggtg cgacaatggt catgattggt tcattgtttg ccggtcacga
    52681 ggaatctcct ggcgaaacaa aagtcgaaaa tggaatcgtg tataaagaat actttggttc
    52741 tgcttcagaa ttccaaaaag gcgaaaaacg aaatgttgaa ggaaagaaaa tctggatcca
    52801 acacaaagga tctttgaaag atacactagt ggaaatgcaa caagaccttc aatcatctat
    52861 ttcttatgct ggaggaagag atctggaagc cattcgaaaa gttgactatg tcatcgtgaa
    52921 aaattcgatt tttaatggag acacaatcta aaaaataaat aaaatattag gatgttgtga
    52981 aaaagttgtc ctgcattaag taataagaac agccaaacaa aaatagttct ttatatgttt
    53041 tgtttggctg gaattattat cgcagataca agcttttgaa catcattatt cagaataagg
    53101 gactgtgata agatttttgt cacagtccct tatgttttgc aaaaaaagtt tttctttttt
    53161 ctcgtaaatt cggtaaaatc aaaagaaaaa atagacaata gaaaagagaa gcttatgtgg
    53221 gaaaaagtag agaaaaaaca aggaccgatt tatcgtcaaa ttatggaaca gattatgaga
    53281 gaaatcgaaa acggaaggat caatcctgga agtcaacttg aatcagaacg aaaactaggg
    53341 caactttttg gtgtgaatcg ttccaccgtt gtccatgcat tggaagagct tagggagctt
    53401 ggggtactaa cgagcaaacg tggcagcgga tggtttgtta atcagactga atgggggaaa
    53461 ttcgctgttc caagaatcga ttggcggcaa atgatctctc ctcgttatga gcagacggac
    53521 tggtatgagc aaaaaataag agaagcaaag cagatccaaa aaacatcgtt tcttgattta
    53581 tacgctagcg agatgccaga tgagcttttg ccgaattttg agtttccttc ttattctctt
    53641 gaagaaatct tagcggaaga aaaagaaatg gatcttttag gctatactcc gttacgtaac
    53701 aaaatcaaac ggtatttgga aagtaaattt gaattggcat tttcccccaa ccagcttctt
    53761 gtgactggtg gcggtcagca agcaatcttt ttgattttac aaacgattct ttcagctgga
    53821 gaggggatag ctgtagagtc accttctttt ttttaccgat tacctctttt caaagcttca
    53881 ggaacgcgtt tatttgggat accgatggat gaagaaggga tcgacttaga aaaactgaaa
    53941 gaatcgatcc ataaaaataa attgaaagca gtgcttatca acccgaattt tcaaaaccct
    54001 acaggaaaag tgatgagtca aaaacgacga gaaaagctag tagaattgtg tcgacagtat
    54061 cggcttccaa ttattgaaga cgatgtattt acggatcttt catttctaaa ccaagaagaa
    54121 gtccttccta tacgaacgat tgatccggat aatgtgttat acgtcggttc gttatctagg
    54181 gttttaggaa aaacaacgaa aatcggttgg gtgataggac ctgagacttt tatctcgcaa
    54241 ctagctcaag cgcaagaaat gatggagttt tcgatgaata tccttacaca aatcacagtg
    54301 gctaatgtat ttaatgaagc aattgatttg aaaatgcaag atgtaagaaa aaagttgaaa
    54361 gaaaaaagcc gactgataag cgcttgggga gattcacaat cgtttttcaa gcttcattca
    54421 attttaggtg gttactatgc ctggattact tgggatggga aaaaactaga ccccgaacta
    54481 gcagaaaaag tagtagatat gggtcttgga attgcaccaa gtctactttt tgggcaagat
    54541 ataaatggaa tcagaatcaa ttacagtagg ctggatgaaa accaaatacc gttgttttat
    54601 tcaaagatga aaattctaga aaagtggtta acagaataat ttcacaaagt aaatagatta
    54661 aagaatataa cagcttggat ttaaatttca agctgtttct tttttttcgg aaaaagaata
    54721 cgatataaag aaactaagaa aaaaccatta tttgtacgtc cgttcacgat gtgtaaataa
    54781 aataaatgtt tgcaacaaaa ctgtataaca ccaacgtttt taaatagaaa cagatggttg
    54841 taagtttgat tagaaaatag aaaaaatgca gatgaaatat aaaacgcttt ccttattgtg
    54901 attttattca ctaatgctgt taatatgcgg attggcataa tttcctttca tcgtgaaaat
    54961 ttgtgctttt agcttgtatt tctcataaat ccatgataat ttattaatga agacggttaa
    55021 atagtgatac agttttattc actgtttcaa tcgcagaatg aagagatttt cttatagatg
    55081 agagaggaat gtgtgacaaa tggcaaacaa aaaagcaccg atcgacttcc aagcactgct
    55141 agatgaagtc aatgcagatt tcccagtata tcaagcactt gatcaagatg gaaacgtagt
    55201 taacaaagat ttagtacctg atttatccga tgatgaactc gttgaattaa tgactcatat
    55261 ggtatggtct cgtgtattag accaacgttc aacagcgctg aaccgccaag gacgcctagg
    55321 cttttttgca ccaactgctg gacaagaggc aagtcagctg gctagtgctt acgcatttga
    55381 taaagaagat gttcttcttc caggttatcg ggatgtacct caactagtaa aacatggttt
    55441 acctttatct caagcttttt tatggtctcg aggacatgct gcaggtaatt cttatccgga
    55501 agaattgaaa gctctaccac cacaaattat catcggtgca caatatgtac aagcagccgg
    55561 tgttgcatta ggtttgaaga aacgaaacaa gaaaaatgtc gtcttcactt atactggtga
    55621 tggcggttca tcacaaggtg atttttacga agcaatcaat ttcgctggcg cttaccatgc
    55681 aaatgccgta ttttatatcc aaaacaacgg gtatgcgatc tctacacctc gtgaaaaaca
    55741 aactgcagca aaaacacttg cacaaaaagc tgtcgcagct gggattcctg gtattcaagt
    55801 cgatggaatg gatccgcttg cagtatatac tgtatctaaa atggcacggg aatgggcgat
    55861 cagtggaaat ggaccagtcc taatcgaaac actgacttat cgttatggac cacatacact
    55921 atctggtgac gatccaaccc gctaccgtac aaaagacaca gatgacgatt ggcagaaaaa
    55981 agatccattg atccgcttcc gtaaatattt gacggaaaaa ggactttggt ctgaagaaaa
    56041 agaagaacaa gtcatcgaag caactaaaga agaaatcaaa gcagctattg ctgaagccga
    56101 caaagtacca aaacaaaagg tttcagattt cttgaaaaac atgttcgaag tatctccaca
    56161 atcaatcaaa gaacaaattg caatttatga agcgaaggag tcgaaataat catggcacaa
    56221 aaaacaatga tccaagcaat cacagatgcc ttagcacttg aactagaaaa tgacgagaat
    56281 gtcgttgttt ttggtgaaga tgttgggaaa aacggaggag ttttccgggc aactgaagga
    56341 ttgcaggaaa agttcggaga agaccgagta ttcgatactc ctttagctga atctggaatc
    56401 gcaggattga gtttcggtct tgcgttagaa ggatttcgtc cagttcctga aatccaattc
    56461 tttggtttta ttttcgaagc aatggacgaa gtagtagctc aaatggctcg tacaagatat
    56521 cgaatgagcg gcactagaaa tctacccatc acaattcgtt cgccatttgg tggcggtgtt
    56581 catacaccag aattgcattc ggataattta gaaggtttga tcgctcagtc tcctggtatt
    56641 cgagtagtga ttccttccaa cccttatgat gcaaaaggac tattgatttc ttctattaga
    56701 agtaacgatc cggttgtttt cctagaacac atgaaactat atcgctcatt ccgagaagaa
    56761 gtgccggatg aagcttacga agttcctttg gataaagcag cagttactcg tgagggaaca
    56821 gatgtatcta ttatcacata cggagcaatg gtgcgtgaag caatcaaagc agcagacaat
    56881 cttgcaaaag aaaatatatc tgttgaaatc attgatcttc gtacagttgc gccattagat
    56941 gtagaaacga tcattcaatc cgtggaaaaa actggtcgcg tagttgttgt ccaagaagca
    57001 caacgacaag cgggcgttgc cgcgcaagta gtttctgaga tctcagaacg cgctatcctt
    57061 tcattagaag ccccaatcgg acgagtttct gcacctgata cagtcttccc atttggacaa
    57121 gcagaaaatg tctggttgcc aaatgcaaaa gatatcgaag acaaagtaaa agaaatcgcg
    57181 gaattttaag aaaagaaggg acgaaacaaa gtcatggctt atcaatttaa att
//
LOCUS       DACDYI010000010        56389 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.010, whole genome shotgun sequence.
ACCESSION   DACDYI010000010 DACDYI010000000
VERSION     DACDYI010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.010"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            330..1175
                     /locus_tag="IWM52_000567"
     CDS             330..1175
                     /locus_tag="IWM52_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9972845.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1456..2676
                     /locus_tag="IWM52_000568"
     CDS             1456..2676
                     /locus_tag="IWM52_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAP9972846.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2673..5393
                     /locus_tag="IWM52_000569"
     CDS             2673..5393
                     /locus_tag="IWM52_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9972847.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5377..6321
                     /locus_tag="IWM52_000570"
     CDS             5377..6321
                     /locus_tag="IWM52_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9972848.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6390..7400)
                     /locus_tag="IWM52_000571"
     CDS             complement(6390..7400)
                     /locus_tag="IWM52_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9972849.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7580..7924)
                     /locus_tag="IWM52_000572"
     CDS             complement(7580..7924)
                     /locus_tag="IWM52_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP9972850.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7917..8351)
                     /locus_tag="IWM52_000573"
     CDS             complement(7917..8351)
                     /locus_tag="IWM52_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAP9972851.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8489..9226
                     /locus_tag="IWM52_000574"
     CDS             8489..9226
                     /locus_tag="IWM52_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9972852.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9219..10430
                     /locus_tag="IWM52_000575"
     CDS             9219..10430
                     /locus_tag="IWM52_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9972853.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10637..11416
                     /locus_tag="IWM52_000576"
     CDS             10637..11416
                     /locus_tag="IWM52_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAP9972854.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11486..12148
                     /gene="trmB"
                     /locus_tag="IWM52_000577"
     CDS             11486..12148
                     /gene="trmB"
                     /locus_tag="IWM52_000577"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAP9972855.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12246..14000)
                     /gene="ade"
                     /locus_tag="IWM52_000578"
     CDS             complement(12246..14000)
                     /gene="ade"
                     /locus_tag="IWM52_000578"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAP9972856.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14132..15061
                     /gene="cpaF"
                     /locus_tag="IWM52_000579"
     CDS             14132..15061
                     /gene="cpaF"
                     /locus_tag="IWM52_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAP9972857.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            15018..16067
                     /locus_tag="IWM52_000580"
     CDS             15018..16067
                     /locus_tag="IWM52_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAP9972858.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16064..16342
                     /locus_tag="IWM52_000581"
     CDS             16064..16342
                     /locus_tag="IWM52_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAP9972859.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16317..16787
                     /locus_tag="IWM52_000582"
     CDS             16317..16787
                     /locus_tag="IWM52_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAP9972860.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16804..17106
                     /locus_tag="IWM52_000583"
     CDS             16804..17106
                     /locus_tag="IWM52_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972861.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17123..17494
                     /locus_tag="IWM52_000584"
     CDS             17123..17494
                     /locus_tag="IWM52_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAP9972862.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17466..17825
                     /locus_tag="IWM52_000585"
     CDS             17466..17825
                     /locus_tag="IWM52_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972863.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17997..19004
                     /locus_tag="IWM52_000586"
     CDS             17997..19004
                     /locus_tag="IWM52_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9972864.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            19029..20213
                     /locus_tag="IWM52_000587"
     CDS             19029..20213
                     /locus_tag="IWM52_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAP9972865.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20650..22233)
                     /locus_tag="IWM52_000588"
     CDS             complement(20650..22233)
                     /locus_tag="IWM52_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9972866.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22811..22954)
                     /locus_tag="IWM52_000589"
     CDS             complement(22811..22954)
                     /locus_tag="IWM52_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAP9972867.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23177..23656)
                     /locus_tag="IWM52_000590"
     CDS             complement(23177..23656)
                     /locus_tag="IWM52_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9972868.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23729..24013)
                     /locus_tag="IWM52_000591"
     CDS             complement(23729..24013)
                     /locus_tag="IWM52_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972869.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24091..24285)
                     /gene="ssrS"
                     /locus_tag="IWM52_000592"
     ncRNA           complement(24091..24285)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IWM52_000592"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24357..25643)
                     /locus_tag="IWM52_000593"
     CDS             complement(24357..25643)
                     /locus_tag="IWM52_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAP9972870.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25781..26452
                     /locus_tag="IWM52_000594"
     CDS             25781..26452
                     /locus_tag="IWM52_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAP9972871.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26418..26906
                     /locus_tag="IWM52_000595"
     CDS             26418..26906
                     /locus_tag="IWM52_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAP9972872.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26919..27866
                     /gene="prmA"
                     /locus_tag="IWM52_000596"
     CDS             26919..27866
                     /gene="prmA"
                     /locus_tag="IWM52_000596"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAP9972873.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27867..28631
                     /locus_tag="IWM52_000597"
     CDS             27867..28631
                     /locus_tag="IWM52_000597"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAP9972874.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28864..31077
                     /locus_tag="IWM52_000598"
     CDS             28864..31077
                     /locus_tag="IWM52_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAP9972875.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31088..31534
                     /locus_tag="IWM52_000599"
     CDS             31088..31534
                     /locus_tag="IWM52_000599"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAP9972876.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31705..32964
                     /locus_tag="IWM52_000600"
     CDS             31705..32964
                     /locus_tag="IWM52_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAP9972877.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33053..34000)
                     /locus_tag="IWM52_000601"
     CDS             complement(33053..34000)
                     /locus_tag="IWM52_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP9972878.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34394..35686
                     /locus_tag="IWM52_000602"
     CDS             34394..35686
                     /locus_tag="IWM52_000602"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAP9972879.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35701..37470
                     /gene="aspS"
                     /locus_tag="IWM52_000603"
     CDS             35701..37470
                     /gene="aspS"
                     /locus_tag="IWM52_000603"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAP9972880.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37579..38454
                     /locus_tag="IWM52_000604"
     CDS             37579..38454
                     /locus_tag="IWM52_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9972881.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38720..40603
                     /locus_tag="IWM52_000605"
     CDS             38720..40603
                     /locus_tag="IWM52_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP9972882.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40678..40962)
                     /locus_tag="IWM52_000606"
     CDS             complement(40678..40962)
                     /locus_tag="IWM52_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP9972883.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40952..41686)
                     /locus_tag="IWM52_000607"
     CDS             complement(40952..41686)
                     /locus_tag="IWM52_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAP9972884.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41815..42438
                     /locus_tag="IWM52_000608"
     CDS             41815..42438
                     /locus_tag="IWM52_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAP9972885.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42630..43751
                     /locus_tag="IWM52_000609"
     CDS             42630..43751
                     /locus_tag="IWM52_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAP9972886.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43758..46886
                     /locus_tag="IWM52_000610"
     CDS             43758..46886
                     /locus_tag="IWM52_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAP9972887.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46905..50099
                     /locus_tag="IWM52_000611"
     CDS             46905..50099
                     /locus_tag="IWM52_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP9972888.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50245..50952)
                     /locus_tag="IWM52_000612"
     CDS             complement(50245..50952)
                     /locus_tag="IWM52_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9972889.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            51717..52115
                     /gene="spxA"
                     /locus_tag="IWM52_000613"
     CDS             51717..52115
                     /gene="spxA"
                     /locus_tag="IWM52_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAP9972890.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
     gene            52447..53100
                     /locus_tag="IWM52_000614"
     CDS             52447..53100
                     /locus_tag="IWM52_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAP9972891.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            53326..54324
                     /locus_tag="IWM52_000615"
     CDS             53326..54324
                     /locus_tag="IWM52_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAP9972892.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            54500..56308
                     /gene="pepF"
                     /locus_tag="IWM52_000616"
     CDS             54500..56308
                     /gene="pepF"
                     /locus_tag="IWM52_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAP9972893.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
BASE COUNT        18555 a         9728 c        11686 g        16420 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata
     1201 gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt
     1261 caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt
     1321 tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac
     1381 gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa
     1441 aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca
     1501 tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg
     1561 accgttttat caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt
     1621 gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa
     1681 cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat
     1741 ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc
     1801 gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga
     1861 tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa
     1921 ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac
     1981 tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg
     2041 catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct
     2101 caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa
     2161 ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata
     2221 tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca
     2281 aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat
     2341 acagatgttt tggaacggat acaatcagga gagctattag actatttgtt agaggaattc
     2401 tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat
     2461 acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag
     2521 acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga
     2581 atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt
     2641 aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg
     2701 cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg
     2761 ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt
     2821 tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg
     2881 gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc
     2941 aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag
     3001 aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag
     3061 agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt
     3121 taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac
     3181 aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga
     3241 aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga
     3301 tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg
     3361 cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat
     3421 ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc
     3481 tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa
     3541 cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc
     3601 aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag
     3661 aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag attttggaac
     3721 aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga
     3781 agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc
     3841 tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga
     3901 cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt
     3961 atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga
     4021 aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac
     4081 caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag
     4141 gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg
     4201 gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag
     4261 gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga
     4321 aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac
     4381 gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa
     4441 tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg
     4501 ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac
     4561 aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt
     4621 cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca
     4681 atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat
     4741 ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag
     4801 caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat
     4861 tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg
     4921 aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg
     4981 aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag
     5041 gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag
     5101 aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat
     5161 ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct
     5221 ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag
     5281 aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga
     5341 tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta
     5401 atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa
     5461 aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa
     5521 tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta
     5581 aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta
     5641 acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa
     5701 gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat
     5761 cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca
     5821 aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac
     5881 ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt
     5941 atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat
     6001 acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc
     6061 tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc tcgtattacg tcatatggtt
     6121 ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca
     6181 gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt
     6241 cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc
     6301 tatgtaccaa aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag
     6361 acataagtct tcgctttttt tgttttcttt tattttgatg aatcagttgt ttcttcagtt
     6421 gaagattctt ttgttgaatc agttgatttt gtgtcagaac tctttgttga agctgtagtt
     6481 tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg cactcaacac attttcaaag
     6541 gcatcatctt tgatttttac atttgcatct tttaattctt caccgattac tttagttgtg
     6601 aatgcattgt cacttagttt agtttcagta gcgatatctt ttagttgatc tttgtattta
     6661 tccatgtcat tgcctttatt ttggtttttc accattttga caacgtagta ttctgtcgca
     6721 taagaagtag gatttgttgt agtaataaca tcggagatct caccgtcttt caatttgaaa
     6781 gctgcttctt ttacttctgc aggaatagtt gttgtcgttg agtcgaattt aactttaccg
     6841 ccatcttctt ttgtctctgt atcagttgat ttgtctttgg ctaatttaga gaaatcatct
     6901 ccatcatccg ctgatttttt cacatctttt gcttcatctt cacttgataa tttgatgatt
     6961 tgtgcttcta cttctggatg gaaagatttc caagcagttt tcaaatcgtc atctgtaata
     7021 tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt tacgaatata ttctttataa
     7081 gtatctttcg tatatccagc agcttctaat tgtgattcaa aagtatcacc taatgatttc
     7141 gcttgtttgt cgtattctgc atccacttgc ttatcggtta ctttatcgcc atatttgtta
     7201 ttgaatactt tataaatgat catccgttgt actaaggatt ggttcgatga ttcaagtttt
     7261 gcttcatcat agaaatcaga tacagtgatc gttccgcctt tcatcgttgc gatatcttta
     7321 ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca acgcactagt agctgccaaa
     7381 ataattgatt tttttttcat taaaatttgc actcctgtaa ttagttatta tttttatata
     7441 ttaactatac catatcccat ttcctttaaa aacgttgtcg tggtagtttc aaacaaaatt
     7501 caaaatttca tcacattcct aggaaatacg caaacttttc tcacttatat agttaaatct
     7561 gttgagaaaa taatcattat tatatttgtt caggatactc tggcagatca gcttttagtt
     7621 tttctacttg ttcattgatt tgtgtgatac gtggttcggc ttggaattta aaatcttcga
     7681 tatctttttt gattgcttct tggaaagcag gaatcacaga ttcagctgtt tcttttgttt
     7741 cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa atcatttgtc agagttgttg
     7801 cttcttccaa ttcttcgatc agtttctttc ttgtttcatt cccgctcctg ggcgccaata
     7861 acagacctcc gacagtgcca gcagcagctc caaataacag tcctttgaaa aagtttttaa
     7921 tcatcgcagg acacctgctt tacgattgtt tcttgaatag cttttagttc ttccgatcca
     7981 taactatctt gatgattgac aaagtgaata gaaaaatcat cctctttgct gtagcgaggg
     8041 actaaatgga tatgtgaatg aaaaacagat tgataggcta gttcacggtt gttattcaag
     8101 atattaagac ctttcatttc tggaaaagct ttttccaatg cttgagcaat cttaggcaca
     8161 cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa aaatatctgc tacatgacgt
     8221 ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga tgtctaaaaa agcatagacc
     8281 ttgtcgtctt catatatttt atagcttggg atttcttgat tgatgatttt gcaaaaaata
     8341 cagttttcca tataaaacat cctttctata aagaataaat acttcagctt aactttacca
     8401 taagttaaag ctattgtaag gaaaaaaaca aaataccgtc attttcttcg tggtataata
     8461 aataaaaata aatggcggag gaaaaagaat gagtttaatt atcgatcatg tcacaggggg
     8521 gtacggacat ctccctgtgt taaaagatat cagtttcgat gtacagcctg gagagatagt
     8581 agggttgatt ggactgaacg gagcagggaa aagtacgacc atcaagcaca ttatcggtct
     8641 gctggaagct tcaaaaggaa cgatagaaat caatcatcta tccttaaaga aaaatacgga
     8701 agaatatcgc aaacaaattg ggtatattcc agagactcct tctttatatg aagaattgac
     8761 attgagagaa catatcgaag tcactgcgat ggcctacgat atacctatgg atatagcaat
     8821 gaaaagagca gaaaagttgt tggagacttt tcggttatct aatcgtcttg actggttccc
     8881 agctaatttt tcaaagggaa tgaaacagaa agtgatgatc ttatgcgcct ttttgatcga
     8941 gccttcttta tatatcatcg acgaaccatt tttaggcttg gatccactag ctatcaatgc
     9001 attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc ggtattttga tgtctacgca
     9061 tattttagct actgcagaaa aatattgtga tcgttttgtg gtcttacata acggagagat
     9121 acgagcagac ggttctcttg agatgttgag aaaagaattt cagctgccgg attcttcttt
     9181 ggacgaaatc tatattgctt taacaaagga gcaggcacat gagtagtttt tttcaaaaac
     9241 gtttagcaag acatcaaaag aaaatgatga aatatatgcg ttatgtattg aacgatcatt
     9301 ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt atattattct aattggttga
     9361 aaaccctgca gccaccgttt gcaattggag ggctcataat ctctgtattt tggatgctct
     9421 gtttattcgt gggtaagatg gctacttttg cagaggcagc agatattgtt tttttacttc
     9481 ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt tcgctattct tgcatttttc
     9541 cgttgatctt tttgatattg gcatgcggat tggccatgcc attagtggtg gttagtacgg
     9601 agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt atggagtttg aaattcagtc
     9661 atctgcaaat caagagagca gaattgtttc gactagatca acaagttatt cgttattgga
     9721 aattaggctg gtttttttct tctctgatct ttttgtttct atcattttat tggctaatat
     9781 gggcaggaat agcaggtgca ttagtacagg caagtctata ttactggttt ttgtggaagc
     9841 aacaagaaaa aggattggat tgggagaaaa tggtgcatgt agaagaacat aggttgcatc
     9901 agatctatca atttatcaat ttattcacag atgttcctga aattacggca aagatcaaaa
     9961 gaagacgcta tttggatcct atattgactc atattaaaag agaatcaaaa aacacttatc
    10021 tttacttgtt tgcaagaaga ttggcaagag gttctgattt tagtggtctt tatttccgat
    10081 tagtaattat aggtgggtta ttgattgccg gagtagtcga cttttatttt agtctgatta
    10141 tcggtttctt atttttatat ttgatcggtt tccaactgtt acctttagcg aaccagttcc
    10201 gctatatggt tctcgttcag ctttatccaa tccataagaa tcagaagaag caggcgattc
    10261 agtatctact cacatgggta ttaattatcg cgtcggttat ttttggtttg attgctgcag
    10321 tcgtattaga tgggcagcag cgatgggttc cgctagcggc ttatttgatt gttactatgc
    10381 tctttgttaa actatatgta ccaactcgtt tgaagaagat gtcagactga aaacaaagct
    10441 gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca gtaagaatgc tgttcgttct
    10501 attttttttg agaaaagctg gttttttgaa tggcaattaa atgaaaaaga acgtatatgt
    10561 gcttgacgtg tagtgttcag gcgactagaa taataagtac tgcaaaacaa agagtaagag
    10621 gacgggagaa tgcatgatgg agtttcagtt agaccaagag tggcggttac gtcccatcaa
    10681 aggcgacaca ggaaaaacat acataggcat gcgtgatgag gacaaagtct ttattaaacg
    10741 aaacacgact ccgatgttgg ctgctctttc aagggaagga attacaccaa aacttgtttg
    10801 gacaaaacga acggggaatg gcgatacttt gacagcacaa gagtggctgg aaggtcgctt
    10861 gctgactcct aatgagattg gtcaaagaaa cgacgtgatt gatgttttgt accatttaca
    10921 tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt ggtcgtgtca tgacgccgga
    10981 gaagatgctg caaaaatatg aaaaagaatt accggaagaa ctccagaaaa atcaatttct
    11041 gcaaattgtc catcgttttc ttcgtgagca gatcccgtca atagataagg aaaacttatc
    11101 agtagtacac ggagatgtaa accatcgtaa ttggttagta tgtaaaaatt atttatatct
    11161 agtcgattgg gattcaatca tgtttgcaga ccctgcggtg gatattgcaa cgattttagg
    11221 aaattatgtg ccactgtcta gctggaatca gtggctgatc agttatggca tacgcccgac
    11281 tgacgaaatg cttgaaaaat ttcattggta tgcggccatg aatttgctcc aagaaatcac
    11341 cagatattgc aaacgtgaag atcatcgacg gatgaacgaa gaaattttac aattaaaacg
    11401 aattttcagt ggctgaggtt tggcccaaac tctgcggaga tttggccaga cctttttaat
    11461 tagaggaaag gaatggagat aaataatgcg tgtccgaaat cgtccgggag cagcggaaat
    11521 gttagcggct cacccgaatt ttgtaattag tgatccaaca ttgtggaagg gaaaatggaa
    11581 tgaattgttt gaaaacgatc atccaatcca cattgagatt ggtatgggga aaggccagtt
    11641 catcactgga atggcaaaag cccatcctga aatcaattat attggagtag aaatgcaggt
    11701 cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa ccgcttccta acctgaaact
    11761 tctacatgtg gatggatcag cgttgacaga gtatttcgct gacagcgaag tagatcaaat
    11821 ctatctgaat ttttccgatc catggccaaa aaaacgtcat gaaaaacggc gattgacata
    11881 taagactttt cttgctgtag atgaacagat tcttcgtcca aatggagaaa tccatttcaa
    11941 aacagataat caaggattgt ttgagtattc tctggcaagt ttttctcaat atggcatgat
    12001 cctcaaacag gtatggttag atttacatca aagtcaattt gaaggcaata ttatgacgga
    12061 gtatgaagaa aaattttctt caaaaggtca acgtatttat cgagtagaag ctcaatttca
    12121 agataagacc aaaaagaaaa gtctgtgaaa ctaactttct gctttcataa aaacattaat
    12181 atccataaat aaatcccaga aaatgaacat tacagatgta atgtattttc tgggatttat
    12241 ttgatttaaa atgtatttat ttcatcggtt tttccacaag gtggaatcaa ttggaagcga
    12301 gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg gtatgacaga taatgttaag
    12361 aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc ctagttgatg tgctttagtt
    12421 attaattcat tcaactgttc atagacgatc gaaaaaggtt cctgtgtcat gattccgcct
    12481 ataggaagag aaacacaagc tatttcctgc tgtttgttga caacggtcat cccgccacct
    12541 ttttgaatca aaagattagc agcatacagc atatcctcgt cgtttgtccc aacgacaact
    12601 agattatgcg agtcatgggc aatcgttgtc gctatcgctc cttgtttcag ctgaaatcct
    12661 ttcactatgc ctaatccaat gtttcccgtt tgatggtgac gctcgatcac cgcaatcttt
    12721 aattggtctt tttcgattga aggaatgaat cgttcgcctt gtcggtccac tttttctacc
    12781 aaatctttag tcactagact attcgggata atttcgatca catgggcttg tgaagaagaa
    12841 agaagcagct ctaacgaatg tggatttaat ggttgagcgt ttagttttgg cagacaacac
    12901 gtatatgaat ttgaatgatc agcaaataaa tggtcttgga gttgcccaga ttgaacaact
    12961 aatgttccct tatagaaaac ttcatgaatt ggcagcttgg taagatcgtc tgttaagaag
    13021 aaatcagctt gatacccagg ggcaatcgca cctagtgttt tcaaatcaaa acattctgct
    13081 gcattcaatg ttgccatctg aatggcttgt aatggatcga ttccaagtgc aatagcttga
    13141 cggacgatat gatcgattga tccttctttt actaaatcat tgatcagttt atcatcggtg
    13201 acaaagaagc atcttcttga attttcaggt gtgatcgccg gtagtagcga tgccagatct
    13261 tttgcaacgg tcccttctcg aaccattaaa tacataccaa gatccaaacg ttctttagct
    13321 tcttttgcag ttgtagcttc atgatccgtc cgaatacctg ctgcaggata aacatttaac
    13381 tcttccatgc cgataccagc tgcatgcccg tcaatttttt tcttatgctg gtgcatcaat
    13441 tggatctttt ctaggatgtc tgtttcatta tttgctactg cttgatagtt catgacttca
    13501 gctaagccta aaacttcggg tttttgtaaa aaaggggcta atttttttgc atttaaattt
    13561 gcaccattgg tctcaaaagg ggttacaggt acacaagaag gtagcataaa aaagatattc
    13621 atgagtgttt gtcttgcatc ttctatcata aactcaagac ctgtactccc agcaatgttt
    13681 cctatttcat ggggatctgt cactacagta gtaactccat gaagcaagga tacttttgaa
    13741 aattcgcttg gtgataaaag tgagctttcg atatgcacat ggccatcgat gaatcctggc
    13801 actagatatt gcccattagc attatgtacg ctttttcctt cgtattcacc gattcctacg
    13861 atcattcctt cgcagattgc tacatctttt tccatgattt ctttcgtaaa gacgttgacg
    13921 atcttagcat gcttgatcac taggtcagca ggcttttttt tacttgctac ttcgatatat
    13981 ttccttaatg ttgtttccaa tggagtgcct cccaagataa atttttataa gtataacata
    14041 aattcggttt ttgccaatat ttttttgtaa attcatttta tttgaaacaa aaagatcaaa
    14101 aagacgtatg ttttttgtgg aggggagaat aatggaaata aaacgattat cggatcaaat
    14161 gataagtgaa agttttgcca aacatgccca agacgtatat atattgccag cttcagaagt
    14221 attttacatc tattttcgct atggaaaaaa acgaacattc tggaagaaag tctcttcggc
    14281 agatggacag caactgatct caagatttaa gtatttggga cagatggata tcggagaaaa
    14341 aagaaaagct cagttaggtg catttactta ttctttagaa aaacaggaag ttcgtctgcg
    14401 gttgtcttct gtaggagatt accttcaacg agagagttta gtgattcggc tattatacgg
    14461 tacttttgaa acaccccatc gttgctttag ttctcgagat tttgcaacta ttttacagaa
    14521 aaccgtcaga cgtggattat accttttcag cggaccagtc ggatcaggca agacttctct
    14581 aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt actatcgaag atcctgtaga
    14641 gatagaagaa ccgttttttc tgcagctaca gatcaatccg aaaatagcac agacttatga
    14701 tacacttttg agactcgcat tacggcatcg tccagacttg ctaatcattg gcgagataag
    14761 agatgaatca actgcacatg ctgcggtaag agcagcacta acagggcacc gggtttttgc
    14821 aaccgtacat gcacgcaatc ttgaaggtac attggtcaga atccatgaac tgacaaagaa
    14881 aaaagaagag ctatctgaat gtttagctgg tgtcatttat caagaacttt tggaagatgc
    14941 caatcaccat ccttctgttt tatggagtta tcaatttatg gaggaagaat cagtaaaaaa
    15001 atcatgggag gaaagttatg aagaagctca ggaaaatatt tggcagtcag aactattcta
    15061 aaggattaac aagaagccag caaaaccagt ttgcacaaac catgggagaa ctgcttttat
    15121 ctggttttag tctgcaagaa tccctttatt tattactcac gatccgttcg tttccacctg
    15181 ttattttggc aaatatccaa catcagctaa aggaagggca agcgtttagt gaggtacttt
    15241 cgcaaatagg atatggagca gagcaactgg tacagatcga gttagctgaa cgtcatggga
    15301 atctgccgac aactttgttg gaaattgcca aacagtttcg gcttgttgaa ggatttcgca
    15361 atgatttgaa aaagctgctt gcatatccga tgctattgct cacctttttg ctaggtatct
    15421 tgtttagtat gcggttgttc gttttgcctc agcttattgg ttcaggaatg gtcatgcaag
    15481 agcattgggg tattcagctg atacaagtct ttcattggta tgttctacta ttcatcctag
    15541 cggttactct attggcttta ctagtaaggt atcgtctgat gaagttgtct cctgttgatc
    15601 agagtgaatg gataagcaga caattattga ttggaccgat atattccttg tatcaatcgg
    15661 cttatttgtc actagagatt ggaaagcttt ttcatgaagg tttcgaatta aggcaaatca
    15721 tagtctgctt aaaggaaacg aaacaagaaa gtctgattca gcatcttgcc tctaagttga
    15781 ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt ccagaattat cgttttctat
    15841 cagaagaatt cagtaaaatt gttttacagg gagaggcaaa gggaagttta ggtaaagaac
    15901 tgatttttta cagcgaatgg acaagaaaac agcttttcaa acgtcttcat cactggatgc
    15961 attttattca gccaatcatt tttttgggcg tagcggcttt gattttatta gtttatgccg
    16021 ctgttttatt acctgtttac ggaagcatag aggaggtttt gccatgaaaa agagaaaaat
    16081 atcttattat agcggtttta cattgatcga aatgctaatc gtcttactga tcatcagtgt
    16141 attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg aatcatgtag accaagaaag
    16201 tcgtgaagcg atcatccagt tggtagacac acaaaaagag ctctatgcgc ttcaaaataa
    16261 tggaagagtt cctactgtgg aggaattgtt gaatgaaggc tatatcaaac gagaacatgc
    16321 agaaatctat cagcggcctt gaaggtaagg ttttagcatc aggatatacg ttgtttgaat
    16381 cactcttact gattttatta cttacaacga tcctttcttt tccagttctc gcattttctg
    16441 catggaagaa tgaattggca gtctatcaat ttttcggcca atttgaaaaa cgaatctatt
    16501 ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg cttttattgg aatagtgaag
    16561 agaatcaaat catttttcat gttccgaatc cagataaaat aaattgggaa gtattagata
    16621 ttcctgatga gattaccttg aaacgccatg cttctattac ttttgctgct agaaccggga
    16681 atgaaagttc actaaaagct tatcaatttt attgggaaga aaaagagcgg acgattacgt
    16741 atcaattcca gatagggagt ggtcgctata caaaaagaat tgaataaaac aggatttgtt
    16801 ttgttggagt cccttacagc gcttatgatc gcttctgtct gtgtctactt agtgagcttt
    16861 ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg aggaactgct atttattcga
    16921 atgatttatg aagatatcaa gacgcagcgg atgtatcaca aaagaccaga gcgaagggaa
    16981 gaaatgatcc catacgatat ccaaattgtc gataataatg gagtaatcga aaaagtgttt
    17041 gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa gtaattccag catttacgct
    17101 gattgaatgt ctaattggac tgatgatatt gagcagtcta ttgctcacat tcaatctttt
    17161 gattcagcaa accatccaag taagtgaatt tttgcagcgt aaagatcaaa aagagtggct
    17221 aatattttta gcacaattag aagaagaact caagagcagt cacaatgtct ctgtgaaaaa
    17281 tcagcaactg cactacacga tcgaggataa acagtatatc atcgaaagat atcaggcaat
    17341 gatacgtaaa agaagaactt ccggcggaca tcaacctatg ttaacaagta tcactgagct
    17401 tcaattattg gaaaaagaac acacaatttc tttttatgta cactttgaaa atggagaaga
    17461 tggatatgcc atatggacaa aaaacgatca gtagaaaatg ttataagggc tctattttat
    17521 tttctgtctt gcttttattt cttttatgta cgtttttatt gatggcagca ttagaaagct
    17581 accgtttgtc aatggatctt aatatacgaa tcaaaaatta ttatatagca aaaataatga
    17641 agcagatggc tttatcggaa cagtctacgc ttgccgaaag ttcggaagga gttttttact
    17701 acacaactgg atctgtaaca tatcagtggg tacaacagtc cctattctta gaggtggaag
    17761 tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa tgatacagat actagtacag
    17821 aatgatggaa tgttcgtgaa aacgccctct tttctgttat aacactgttt ttaataaaaa
    17881 aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat tgaaattttg tcaattgttc
    17941 gatagaatta aacaggtgaa aaaattgtta taatatatct agaaatgagg tgcaccatgt
    18001 taccagaaaa aatggaacaa gggtttaatc aaaacctgga agctattcag cttttacaga
    18061 atgcattggg aacttccttt ttagaagcct atgtcgaaaa tgcagaaaat ttaatagatg
    18121 attatcaagt acgtgtggtg gatggagtgc cgacaaaaga gacaacgcaa cggatcacag
    18181 ctctttatga agagttgaag aaactttctt tcgagccaga agaatggcgc agactgtctc
    18241 aattactttt attgaagggc agccagacag aacatttgca ggcgaaccat caactcactc
    18301 ctgatagtat cggtttttta tttgtttttc ttattgaaca actatacaca aataaaaaag
    18361 caccggttaa gattctggat attgcggcag gaatgggcaa cttgttatta acagttcttt
    18421 taaacctatc gaatgcgggg taccaaacag aaggaattgg tgtggatatt gatgatacat
    18481 tgttggctgt cgcagcttct acaagtgatt tgacacaagc aaatgttcaa tactttcatc
    18541 aagacgggtt gcaggaatta ttaatagatc ctgtcgattt tgcaatcagc gatttgccaa
    18601 tcggttatta tccgaatgac gaaaaagcaa aagagttttt aacaagtacc gaagaaggcc
    18661 atagttatgc acatcattta ttgctagagc aatcaatgaa atatgtgaag ccggatggtt
    18721 ttggtttgtt tttgatgccg agtggatttt tagaaacgga tcaaagtgaa gaaatcaaaa
    18781 aatggtttaa agaagaaggt tatcttcaag gaatgattca attgccagat gaattgttcc
    18841 gtaataaaca atcgcaaaaa agtattttga tcttacagaa aaaaggacca caagcacaac
    18901 aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa agaaccagag aaggtgacgg
    18961 aatttttcaa cgagtttaaa aactggaaat catctgctct gtaatcattc aacgagaagg
    19021 agagtcatat gtctaaaaca attgcaatca atgctgggag ttcaagtttg aaatggcaat
    19081 tatatcaaat gccagaagaa accgtagttg cgaaagggat cgtcgaacgt atcggtttac
    19141 aagattctat ttttaccatc aagtacggcg ataatcaaaa attcgaacaa atcatggata
    19201 tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca gttgattgaa ctgcaaattt
    19261 taagctctta tgatgaaatc accggagtag ggcaccgtgt tgttcacgga ggagaaactt
    19321 atggggattc agtagtgatc gatgagaaag taatggaccg cattgctgaa ttagcagaat
    19381 ttgctccttt gcataatcct gcaaacttaa tggggatcaa agcgtttaaa aagattttac
    19441 ctgacatttt gagtgtggct gtattcgata catctttcca ctcaacaatg ccaaagcaaa
    19501 attatttata tagcttaccg gtgtcttatt atgaagattt caaagtaaga aaatatggct
    19561 tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc aaaattattg aacaaaccga
    19621 tcgaagaact aaaaatcatc acttgccact taggaaatgg tgtatcgatc acagcagtcg
    19681 atggaggtaa atccatggat acttctatgg gattcacacc attagctggg gtgacaatgg
    19741 gtacacgttc aggtgatatc gatccagctg ttctcccata tttaatggaa aaattagaga
    19801 ttgatattga tgaaatgatc aatgttttga acaagaaatc tggtctgcta ggcctaacag
    19861 gattgtctag tgatatgcgt gatcttgaaa agaactatga aaaagaacat attcgtttag
    19921 cttatgacat tttcgtggac cgcatccgaa aatacatcgg cggttatgtg acaacgatga
    19981 atggtgttga tgcaattgtc tttactgctg gtatcggcga gaatgacgga catgtacgta
    20041 gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat cgatccagcg ttgaatgaat
    20101 tgcgtggtga agaagtagat atctctacaa aagaatcaaa agtaagagta ttagtgatcc
    20161 caacagatga ggaactaatg attgcccgtg atgtggagcg tctgcgcggc taagccaaag
    20221 cattcgttag gataaaagat gaaacgcatg caagagggaa ttcttgcatg tgtttttttt
    20281 gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc cttctttttt acaggatctt
    20341 cttagggaat tttgcttgga aaagtggtca aaagggaaaa gactggcatt ttatttattg
    20401 attttcagga aaatcccaat attttatttt cattcatctt tataagggac attacatatg
    20461 taaaaagaaa aaacattttt agatcagcct cgtgggctgt cgactttatt cttcgcttta
    20521 tgtttagcta gaaaaccgat ccgaaacatt cattgatcga ataataaaaa cttgattttc
    20581 cacctgtact ttgcctgaat cgtgaaagca caggtatgaa aatcaagttt tttgttgcga
    20641 aaaaacgtcc tagtcaatca gttggccgag cggctggcgg ctgatctgct cctgatcaat
    20701 aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg tcatggctga ctgctaggag
    20761 cgttccttga tagtcttgca gcatttcttc aatttcttct cgagcatagc tgtcgaggtg
    20821 attggttggt tcatccaaaa tcaggacgtt gattttttct aaaaagagtt tcagcaacgc
    20881 caaacggatt ttttcaccac cggataaatc tttgatccgt ttacccacat cgctttgata
    20941 aaagccactt ttggctaatt gccgtcgagc gacttcttcg tttccagtca atgtgatggc
    21001 gtaagccagc aaacggctat cgggatcggc aaactgcagc tgctgcggca aataaccgat
    21061 ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc ttttcctcac cgagtaataa
    21121 tttgagtaag gttgattttc ctgcaccatt gtcgccgatg atggctaaat gatctccacg
    21181 atagactgca aaactgctgt ctttgaatag tacttgatca gcgtactgct ggtgcaagtt
    21241 ttgaatgatg aaaacttctt tgccagattt tccaccatta gacaagcttt gcagccgatt
    21301 tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc aggcggcgtt ccagttcttt
    21361 ggcttttttg aagaaggact cattgtctcc ttcatgtgcc cactgacgaa aacgacgaat
    21421 catgactttc aaccgctgga cttctttttg ctgcagttcg taattttttg tcagctcagc
    21481 caagcgagct tctttcaaga ctcgaaaacg gctgtagttt ccgggatact caatcaatgc
    21541 accttcttcc agttcccatg tttttgtggt gacttgatcg agaaattgcc gatcatgaga
    21601 aacgaccaga accgattttt ttgtattctg taaatagttt tccaaccagc gaatactgtt
    21661 aagatctaaa tgattggtcg gttcatcgag taaaagtaaa tcggcatcgg ctgctaaaat
    21721 tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt tctgttaacc gtgcttcctg
    21781 acggtgtgga atccctaagc cttgcaagat cgctgcaatc cggtcttcta atacgtaacc
    21841 gcctagttct tcaaagcgat gctgcagctc accgtaacgg gccagacact tctctaagtt
    21901 tgtatctggt tctgtcatca atgcttcgta gcgactcatt tgctgtttga ggtcctgtat
    21961 ctcagcggca gaatggagca agtagtggtt gacggtcgct tcggaaactg taagttcttg
    22021 ttcgaccgtc ccgatcttta agcctttttt gcgactgata acaccgctgt cgacaccttc
    22081 tctgccggtc aagatttgta aaaaggttga tttgccagtt ccgttgatac cgactaaggc
    22141 aattttttca ccagcttcaa tttgacagga taaatcctca aataatggat tcccgccgat
    22201 tgttttttta atagaaattg cttgtatgat catgatctct ctccttgaga ccactcaaaa
    22261 aggccatggt tatccatggc ctgtgttatt taaactcaga ttatcatgag aaaaatacct
    22321 tgagatggcc attgatttta cgtgaattca actgttttgg acaaacttgt cccgtcaaca
    22381 gcaactgtaa aaccaattgc atggctaaat gcgatatggt atcgttggta attttcatta
    22441 cttgttattt tagtcatgcg catcatagta ttttccctcc ttcgtgtagt tgtcttttcc
    22501 agtgtacctg aaagtgtcag aaagttcaac tagcgctgac atttacttaa tatttatcga
    22561 tgtgtaattc tgtcagagag ctttttttat ctgactgagg gtaatgtctc gtggaccaca
    22621 tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca tatggtgttt gataaaaaac
    22681 tttgtctaca tgacttcctt ttgccatttg cttaataccg ttgcattaaa cagctcttct
    22741 ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt gtggttgcaa tggcaagcgg
    22801 tagcctttca tcaattcttg aatgatttac cgaatgtagc acttgccttc tcgcggtacc
    22861 cgaacccgaa ataaaatttt agtttggcga taaaatcggt atccgccatc gatacttcat
    22921 attttttcgt atcagattca tcggaaactt ccacattgac gaaccaataa gctttggcat
    22981 gcttaggtcg ttttaaacca caccagggcg actaatcgcc ctggtgttca ctttttttta
    23041 ttttacttga actcattgac tgttttccct ttattttaaa cattataaag accgatgagt
    23101 gcttgccgcg tcttttgttt ttatcacggt tttttgtgca aaaaaacctg ccgatagttg
    23161 gcaggtcgtc tacgtgttaa gctttggtgt ggtctttagc tggcaattgg ttttctaaat
    23221 cagttcggac aacaagcaca tcgcaagctg cgtttctaat aacatattca gaaacagaac
    23281 caataaataa acgttctaca gcattcaatc ctgtggctcc taacataatc aaatctactt
    23341 gattatcttc tgggatttct ctggcaataa tttgttttgg tgagccatat tctacgacag
    23401 aggttacatt attcaagcca gctttcttcg cattgctttc ataatctgcc aatgtttgct
    23461 ttgccatttc agtagcttgt tctgcaagca ttccatcaaa ggatgaaacg gtttggaaag
    23521 aacgtgtgtc aatgacatgt gctaatagaa gttctccgtt atttcggata gctacattga
    23581 ccgccttttt aaaagctaat tcagcttcat cagaaccatc tacggcaacc atgatttttt
    23641 tgtattgttg taacataagc attcactcct caagtcaatc gtattgtact cttttattgt
    23701 agaacacttt agggaaaaag agaattattt actgctttgg ttcagcaatt ttttttgcaa
    23761 atcggatact gaaggtggca gaaacaataa ttacgatcag taaaaagacc aaggcaataa
    23821 agcgttgatt gaaaaaagct gttaaaatac ccattacgcc taaaacagca tatgcagctc
    23881 cgtaaaattg taggaatcct tgatttttat catttttgtt gatcaataca aaaaagatat
    23941 cctgtttatt aaataaataa tagctaacga ataataatac taaagtggcg ataatcatta
    24001 aaatcctaat catgaacaaa tcctccaaaa taagcaacaa atagaaaaag caatttgcca
    24061 agagcaaatt gctttattct ttaaaagatg gtaatctccg ttaatgccgt tgtttgtcct
    24121 gtagtattga tcccgcgaat tcaagcaggt gggtcccatg gtcgtagctt cgaactacca
    24181 aatgataagg catgttacca gcagtgacac aagacgggtc ccaagatatc aataaaaatg
    24241 ttcggtcaac acacaaagag gacaattcga acatcacaga tttcccacct ctatagtagc
    24301 atatgaattt aaaaagagca atgagagagc tcgcagaaac agaaccgttt tcttgtctag
    24361 ttttttggag gatgcttttt ccactctttt atacggtaat actgttgacc cagagcctgt
    24421 tcatatttcc ctgtatcttt tggctgataa tactgagcat tcttcagttt atctggtaaa
    24481 tattgctgat tgacccaagc ctgatcaaaa tgatgaggat actgataacc cacgccacgg
    24541 ttgagtgatt tcgcaccttt gtaatggcta tctctcaaat gatcaggaac atctccagct
    24601 ttgccctctc gaatatcagc aagtgctgca tctaatgcca tgtaggcaga attggatttt
    24661 ggcgacaagc aaagatcgac gacgacgtct gctagtggga tacgtgcctc aggcaatccc
    24721 aatttttcag cagctaatac agcgttcact gtacgagccg cagcagcagg attacctaaa
    24781 cctatatctt cataaccgat aaccatcaga cgccggcaaa tacttgccag atcacctgcc
    24841 tcaactaaac gggcgagata atgaagtgca gcatccacat cacttccacg aatcgatttt
    24901 tgaaaagccg aaataacgtc ataatgagca tctccatttt tgtcatgggt aagagctttt
    24961 ctttggacac attcttcaat gatcgacagt gtaagatgaa tccttccttc tttatctgaa
    25021 ggagtcgata gtgtagccag ttccagccca ttcaatgcac tccgcaaatc gccgttcgtt
    25081 gcacgcgaaa ggtgaagaag agcatcttcg tctaactgga ttgcttgttg tccaaggccg
    25141 cgttctttat cccttaaagc atgttcgact gccaactgga tgtcttgttc tgttaatggt
    25201 ttgacttcaa agatctgtgt acggctgcgg atcgctggat tgatggtaat ataaggattc
    25261 tcagtagtcg ctccgatcaa gatgattcgg ccactttcta aatgaggaag cagaaaatcc
    25321 tgttttgttt tatctaatcg gtgtacttca tccaataaga ggatcactgt accactcatt
    25381 tttgcttctt ccgctacaac ttgtagatcc ttttttgaat ctgtagcggc gtttagcatg
    25441 cgaaatgcat aattagtaga gccggcgatc gcacttgcga tactcgtttt tccagtacca
    25501 ggaggtccat atagaatcat ggaagagagc atgcgagcat cgaccatgcg gcgtatgatt
    25561 tttccttccc cgactagatg ctgctgacca acgacttcat cgatcgttcg aggtctcatg
    25621 cgataagcca atggctgttg cataggattt tcctccttct tgtttttacg tcccttaatt
    25681 atatcatatt tagcaagatg cctagaaaga acagccttct gttgttttgt tttcatagct
    25741 tatccaacta atattgtgta tgataaagta ggtgagaaaa atgacggata aacaaaagat
    25801 agatgaattt ttttggaata aaacgacgcc agaagtggca cgggatttat tggggatgta
    25861 tttggagcat gacacaccag aaggaagatt agctggatat attgtagacg cggaagcata
    25921 cctaggtcca gaagacgagg ccgcccatag ctatggttta agaagaacac cgcgtgtgcg
    25981 ggcaatgtat gaaaaaccag gtacgatcta cctttacacg atgcatactc atcggatttt
    26041 gaacatcatt actcagccag aagggattcc tcaaggagta atgatacgtg caatcgaacc
    26101 cgccgcaatg attgatcaaa tgagtaaaaa tcgtggcgga aaaactggtc cagatatcag
    26161 caacggtccg ggaaaattag tggaagcatt agcaattcct caagaacttt atggtcagtc
    26221 tatagcagat agttcgttac gcttagtttt tgagaaaaaa aagacaccaa aaaagatcaa
    26281 tgctttgcct agaatcggta tcccaaacaa aggtgtatgg acagaaaagc cgttgcgttt
    26341 tgttgtgtcg ggaaatcctt atatctcttt acaaagaaaa aaccaaatcg agaaagattg
    26401 gggttggagg aaagaaaatg aaaaagaagg atctattaac atatttagat gaaataattg
    26461 aaaaacaagt gacagactat gatgtagctt tagattggga tacaaaaaat catacgatcg
    26521 agatcgttgt tcgcttgttt gcagaaaata aagcacaatt tgagattgat gatgcagaag
    26581 ggatcgtttc gcaagaacca attatcgaat ttgaagacgg cgtacttctt tttaatcctc
    26641 agaaatctgt ttttgatgaa cgcaattatc tagcagtaat cccttatgaa ggaaaaaaag
    26701 ggttggcaaa atcagtagct gattctttag tggaatatct aaatgaagta ttagctcaag
    26761 ggcaaagcga tttgcttgat tttttggatg aagacgatga cgaagctgta ttcgagcttc
    26821 actgggataa tcagcaacta gcagaattag tagaagtaaa acagcaaaat gaagcagata
    26881 cttatttacc gtacccaagt tattaaggag tgacagatat gaaatggaat gaagtaaaag
    26941 tagaaactga aagtgaagca gtcgaagcag ttgctaatat tttgatggag gcaggggcga
    27001 gtggtgtcgc gattgaagat tctttagatg ttgaacattt tcagtcggat caatatggtg
    27061 agattttaga taaaaatgat tttacatcgc tgaaagaagg cgcactagtc atggcttatt
    27121 ttccggaaac gactttctta ccggaaatct tgccttttat caaagatcgt atcacacaat
    27181 tacctgagtt tggtctagct attggaaaaa ataaagtaac agttagtgaa gtagaggaaa
    27241 gcaactgggc aacagcctgg aagaaatact atcatccggt gcgtattacg cgaatgctga
    27301 cggttgcgcc aagctgggaa aactaccaga cagaagatcc tctagaaaaa attatcacac
    27361 tagatcctgg tatggcattt ggcacaggca ctcatcccac gacttccttg acattgacag
    27421 cactagaaac agtcttgcgt ggtggtgaaa cattgctaga tgtgggaact gggtcgggcg
    27481 tgctaagtat tgcagcaaaa tatcttggtg caaaagatgt ctatgccttt gatttagacg
    27541 aagtagccgt tcgttcggcg aaagaaaata tggatatgaa tgaaatagcc aaagatgtcc
    27601 atgtatcagc taacgatctt ttgacaggaa tcgagattga aagtgatgta atcgttgcga
    27661 acattttagc agatatcatc ttattgatga tcccagatgc ttggcgttta ctgaagcaaa
    27721 caggcacgct gatcgtttca ggaatcattg aagagaagaa gcaaatggtt ctggatgcca
    27781 tgttagaaca agggtttatc gtggatcaaa ttttccagca aaaagactgg tatgcaatta
    27841 ttttgaaaaa accagaggtg gaatagatgc agcggtattt tttaaaagaa tcatatgaat
    27901 caaaagacta ttttgtttta gaaggggaaa actatcacca cattgtacgg gttatgagga
    27961 tggaacccga ggatgaagtt tttttggcat tcaatgatca aatagccatt gttgcagaaa
    28021 tcacggagat tactgaagag gctgtttatc tgaaagaaat taaaaaagaa gaaaatcaaa
    28081 aggaactgcc aagttcgatt accatagcga gtggctatcc taaaggggaa aagctggatt
    28141 ggatcgttca gaaatccact gaattaggag cgcaagcttt tcttggtttc ccggcatcag
    28201 cctctgtcgt taaatgggat gcaaaaaaac gtatgaaacg acaacaacgg cttgaaaaga
    28261 ttgctcaaga ggcagcggaa caatcccatc ggcagatcgt gccatcagta gagttattgg
    28321 aaacgaaaca agatttattt gagcggctag atgattatga cgtggtcttg gttgcttatg
    28381 aggaatcagc aaagcaaggc gaaaaatcaa atgcagccaa aattcttagt gaaacaaaac
    28441 cagatagtcg aatcttagct gtctttggac cagaaggtgg attatcccgc gatgaagtcg
    28501 attattttgt agaacacaaa gggatacttt gtggattagg accaagaatc cttcgtacag
    28561 aaacagcacc tctttattta ttaagtgctg ctagctatca atgggaatta atgaatcctt
    28621 ctttcagcta acgttcttca ttaataaaag agtatcgaca gaaagaattg aaaaaatagt
    28681 caacaatttg tataatagaa acaattattt atacaatgga tacgaaaaat cgtttgaaaa
    28741 agcttttaaa aagcgaacat ttccgtaaaa aaacgtataa ctgcacggat tttgggacaa
    28801 ttatcaaaca gatatttgtc ccgattcccg ttttaccgaa aaaagaaagg gggatcgatt
    28861 cctgtgacaa aagaagaaat tttaacggga cccggtgtca taaagctcgt tagtcaatac
    28921 atgggtcccg aacatgtagc atttgttgag aaagcatgcg aatacgcgac tgctgcacat
    28981 gacggacagt ttagaaaatc aggcgaaccc tacattattc atcctatcca agtcgcaggt
    29041 atattagcag atttaaaaat ggatccccat acagtggcta caggcttctt acatgatgtt
    29101 gttgaagata cagaaatcac tttagaagat ctgagagaag aatttggcga tgacgttgct
    29161 atgttagtgg acggcgtaac caaattaggg aaaataaaat ataaatccca cgaagagcag
    29221 ctagcagaaa accaccgaaa gatgctgcta gcaatggctc aagatttacg agttatcatg
    29281 gtcaaattag ctgatagact acataacatg cgtacgttga agcacctgcg agaagataag
    29341 caaagaagga tcgctcagga aactttagaa atctatgcgc ctcttgcaca tcgtctaggg
    29401 atcagccgga tcaaatggga attagaagat acagcacttc gttatctaaa tccaaaacag
    29461 tattaccgta tcgtccactt gatgcagacg aagagagaag aacgtgaaaa atacgtaagt
    29521 ggtactgttg aagatattcg aatagcgaca gaagagctgg ggatttttgc agaaatctat
    29581 ggacggccaa aacacattta ttcaatctat cgtaaaatga aagatcagaa aaagcagttc
    29641 aacgaaattt atgacttgct agctattcga gtgatcgtag attcaatcaa ggattgttat
    29701 gctgtattag gagcaatcca cacaaaatgg aaaccaatgc ccggcagatt taaggattat
    29761 attgccatgc caaaagctaa tatgtaccaa tctttgcata ctaccgtgat cggtccggca
    29821 ggaaatccgg tagaaattca aatccgaaca caggaaatgc atgaaatcgc tgaattcggg
    29881 gttgctgcac actgggccta taaagaagga aaaaatgaaa aagtagaacc agatggtatg
    29941 acgaaacaat taagctggtt ccatgagata ctcgaacttc aagacgaaag ctatgatgct
    30001 tctgaattta tggaaggcgt aaaaggagat atctttagtg ataaagtcta cgtcttcaca
    30061 ccaaaaggag acgttactga gttaccaaaa ggatccggac cattggactt tgcatacagt
    30121 atccatacag atatcggtaa caaaaccact ggtgcaaaag taaatggcaa aatggtgcag
    30181 cttgattaca aattgaaaaa cggagatatc attgagatca tgacttctcc aaattcattt
    30241 ggcccaagtc gcgactggtt gaaattagtt gctactagca aagcaagaaa taagatcaaa
    30301 cgtttcttca aagcccaaga tcgagaagaa aatgtgatca aaggccacga atccgtggtc
    30361 aaatgtatta cagatctagg atttacgcct aaagatattt tgacgaagaa caaactgcaa
    30421 gaagcactcg atcgttttaa ttatcaaaca gaagatgatc tctatgcagc tgtagggtat
    30481 ggagaagtta gccccttgac gatggccaat cgtctgactg aaaaagaacg taaagaacaa
    30541 aaaatcgagc agcaaaagca agaagcagaa gaaatcatga atcagccgaa aaaagaacct
    30601 gacaaaatga aagtacgtca tgaaggtggc gttgtcattc aaggggtaga aaacttacta
    30661 attcgtatca gtcgctgctg taatccgatt cctggtgatg atatcgttgg ttatatcact
    30721 aaaggcagag ggatatccat tcatcgtcga gattgtccga atgttcagcc tgacaaacca
    30781 aatgtagcag aacgtttgat tgaagtcgaa tgggaagata catcgaatac acgaaaagag
    30841 tatgatgcag atttggaaat ttacggctat aatcgttcag gcttattgaa tgatgtactt
    30901 caaacagtca atgcgctaac gaaaaatctc aacagcgttg aagcacggac gaataaagat
    30961 aaaatggcga cgatccattt gacggttggt atccagaatt tatcccacct aaagagtatc
    31021 gtggataaaa tcaaagcagt acctgatgtc tacagtgtac gccggacgaa tggataggag
    31081 gacagatatg agagcagttg ttcaacgggt aagtaaagct gctgtgtcag tcgatcagca
    31141 agaaattgga aaaattggct acggcctact tgttttatta ggcattcatg aaaccgatac
    31201 gcaaaaagat gtggactatc tggtaaaaaa aattgcacaa atgcgtattt ttgaagatga
    31261 acaaggaaaa atgaatatta gtgtagaaga tgtgggagga caaattttaa gtatctctca
    31321 attcacactg tttgcggata cgaaaaaagg aaatcgacca agctttgtgg aagccgcacg
    31381 accagaaaca gctattcctt tatacgaatc attcaatgaa ggaatcagaa gtcgcggcat
    31441 cacagtcgct accggacaat tcggagcaga tatgtcggtt tctctcgtca atgatggacc
    31501 tgtaacgatc attattgatt ctaaaaacaa ataaagtaat ctggttttca ctagtaaaaa
    31561 ctatgaaaca cgtcgcttaa gttagactga taaactctaa cttaagtggc ttttttgtct
    31621 gcaaaaagcg cttcttttgt cagcagtcgg aaaaaaagtt aagcttaaat tatacaagtt
    31681 attttttata ggaggttgct gaagatgaaa gaagtaccag tagtagatat caaaatcaca
    31741 gatttaaaaa aattgtatca aaaaggatac aacatgttgg aggagttacg ccatgaagcg
    31801 gacgctcccg tcgtcaaagc aaagattttc aataaagaag ctatcacgat ctacggatca
    31861 tcagcagcaa aagtatttta tgatccaagg aatttcaaac gaaaaggtgc tatgccaaaa
    31921 ttagtattga agacactttt tggtcaaggt ggagtacaga ctttggatgg agctgctcat
    31981 caccatcgga aaaatatttt tatggatcta atgacaccgg aaagaatgga agattatcat
    32041 cgtatcttag ataagaatct gacacaagca cttgaagcac agcatggtca atttgaacta
    32101 tttgatttaa gtaagatggt tttctttaca tctatttgcg agtgggcagg tatcaattta
    32161 tcagcgattt ctaaagatga ggtcgaaaaa ttagcagaat atcaaatctc tatgatcagc
    32221 ggaacgttta cttctccgat tgatcacata aaaggtgtcg aaaatcgcaa aaaatcagaa
    32281 aaatgggcgc aaggcttgat tgaagaagct cgacaaaatc ctgtggctgg aaaagaaaat
    32341 gtggcattat atgcttttgc caatgccaca gatttagatg gtcagctttt acctctggaa
    32401 gtggcagctg ttgaattatt gaacatcatc cgtccaacgg ttgctctaac tgtgtgggcg
    32461 gcattgatgg gacatgcact tttcagtaga ccagatttgt atcagcaatt aaaaaatgac
    32521 ttttcgacgt tacaagatcc gtttattcaa gaaatgagac gctattatcc tttcttccca
    32581 atgctacctg cgatttcgct gaaagaagta gaagtagatg gttatcgtat accagaaggc
    32641 agttgggtta ttttagattt atatggaaca gaccatgatg aacgaacagt tgaagcgcca
    32701 gattccttta tgatcaaacg gtatgtaggg aaagcaaaag atatttccta taaagaagaa
    32761 tatgagatga ttgcacaagg cgggggaaat tttcgacaaa tgcatcgctg tgcaggagaa
    32821 tggatcactc ttcatagtct acgtgtgttt tccgatcagc tagtaaataa gtttgagttc
    32881 tctgtacctg aacaagattg gacgattcca ttcaatcagt ttccgactta tccgaatagc
    32941 cgagcgctac tttacaagaa ttagattagc ataaacttat cgaaaaaaag gaagatcaaa
    33001 cagtgctgat cttccttttt tcatattact ataaattgat aaggtcaatt gatcaaaatc
    33061 cacgaaatgc ttcatcaatg gtttgataat cttcattgga taaatgcaaa tctaaagctt
    33121 ggacattgtc agatacttgc tctggacgct tagctccagg tataactaca gacactcgtg
    33181 gattcttcat gtaccaagct aaaacaagtt gcgcaggagt ggcttggtat cttttagcaa
    33241 tggggcgaag tacatcgaca gcggttaaaa tttctctgaa acgttctcct tggaaatctg
    33301 gatttctttt tcgaggatct ccttcaccaa attgtttttc ctcaccaagt tcatattttc
    33361 ctgtcagcaa accggaagca agaggaaaat agggaacaaa actgattttg tttttttcaa
    33421 gataagggaa taattctttt tcagcctgcc gatggatcaa actgtattta tcttctacta
    33481 catcgacata tccatctgca ttcgcttctt tcaattgttc taacgtgaag tttgacacac
    33541 cgactgctcg gatttttcct gcttctttta attcgtgcaa tgtagcaact gattcatttt
    33601 ttggtgtgct ttcatccgga aagtggatat aaaaaatgtc gatgtaatca gtttgcagac
    33661 gttttagtgc atcttccacc gattgtttga gaaactcagg agaattatca aatgttcgcc
    33721 ctttatttgg tacatgagca gcttttgtag cgatgacgac acgtgagcga tcatattctt
    33781 tttccttcag tacctcgcca atcagttctt cggaacggcc gtttccgtaa gcataagcgg
    33841 tatcgatcaa attgatcccg ctattcaaag ctgtacgaac tacttgtttt cctgtctcat
    33901 cttccaaacc gtcaaaaaga ttatgtcctc caacggcatt tgctcctaat ccaagctgtt
    33961 ctgcgtagac ttgtgaatgt ccgattctta tttctgccat agctgattcc tcctttttgt
    34021 ctagtctaaa acaaaatacc aaaaagctga actaaaacgt ttttgtgagg ggaaatagtc
    34081 agatatctct tgactttgag actggttttt agtaggttat aagtaatatc gaatgtcaat
    34141 gaaagagcga gtaagttttt gaaactttac aagagaggat catcgaggct gaaaatgatc
    34201 taaagtttca ttagcctaaa gacactctgg agatggacga gtgaaaagcc gtcccgcgta
    34261 cgagcgttat tcgtaagagg aaagagaaat ctttcaaaat taggtggtac cgtgtatgaa
    34321 tttcattcac ccttgttcat gatgaacaag ggtgattttt ttatttacat agctaagaaa
    34381 gggagagaaa actatgagtt tccaaagacc aaaaggaaca aatgatattt tgccaggcga
    34441 atcagaaaaa tggcagtttg tcgaagaaac agcacgttta ttattcagag attatcagta
    34501 taatgaaatg cgtacaccga tttttgaaca ctatgaagtg atatcccgaa gtgtagggga
    34561 tacaacggat atcgtttcaa aagagatgta tgatttttat gacaaagggg aacgccatgt
    34621 gacgcttcga ccagaaggta cagcaccaat cgttcgttct tttgttgagc ataaattgtt
    34681 cggcccagag tatgcgaaac cgttcaaaac tttttatatg ggaccaatgt ttcgctacga
    34741 acgccctcaa aaaggacggt tacgtcaatt ccatcaaatc ggagtagaag catttggatc
    34801 agaaaaccct gcaactgatg tggagagcat ggtgatggca cttgatttct tcaaacagtt
    34861 ggggatcacg caaatccggc tagtcatcaa ttcattaggt gataaagaaa cgcgtcaagc
    34921 ctatcgtcaa gcattgatcg actatctttt accttttgaa tcagagctga gtgaagactc
    34981 taaacgccgc cttcatgaaa atccattacg cgtattggac agtaaagaca aacgagatca
    35041 aaaatttgtg gcagaagctc catctatctt agattatcta agcgagcctg ctcaaaaaca
    35101 ttttgatacc gttgtttcca tgctggatgc attggctgtt ccttatgaaa tcgacagtaa
    35161 tatggttcgc ggactagatt attacacaca tacgattttt gaaatcatga gtgacgcacc
    35221 aaaaatgggg gcacaagcaa cgatttgcgc aggcggacgt tatgacaatc tagtggaaga
    35281 actaggtggt ccttcaacac ctggcttcgg atttgcaatg ggtatcgagc gattactctt
    35341 gactatggag gcagaggaag tagttatccc tgcatttaat gagttggatg cttacgtggt
    35401 cgcactcgga gacgaaacaa atatcgaagc attgaaagtg gtccaagcga ttcgaaactt
    35461 tggtttttca gctgaccgcg acttcatgaa ccgtaaagca aaagcgcaat ttaagacagc
    35521 agataaagcg aatgccaaat tagtattggt gatcggtgaa gatgagcttg ctagtcaaac
    35581 gattaatgtc aaagcaatgg caagccgaaa agaaaaaagt tttccattag aagagatata
    35641 tgaacatttt gatgaagtct atgatgaaat gactttattc gagtaggagg aaaaaaataa
    35701 atggcacaaa gaacgatcta ttgcggatta gtttccgaac aacaattaga acaaacagtt
    35761 acattaaaag gatgggtaca aaaaagacga gaccttgggg gagtaatctt tatcgaccta
    35821 cgtgatcgtg aaggaatcgt tcaaatcgtc tttaatcctg aaatcaacaa agaagcatgg
    35881 gaaatcgcgg ataaatgtcg cagtgaatac gtgatcgaag tgactgggga agttagaaga
    35941 cgcggagaaa atgcagtaaa tcctaaaatg gccactggtg aattcgaagt gattgcaagt
    36001 gaaatcgtga ttttgaatca agcaaaaacc ccgccatttc aaattgaaga tgatcaagca
    36061 atcagtgacg aaatacgttt gaaatatcgt tatttggatc tgcgtcgtcc aaaaatgact
    36121 aaaaacatca aattaagaaa tgcgacaaca aaagcgattc gtcattattt agatgataat
    36181 gactttctcg atatcgaaac accttaccta gggaaatcta caccagaagg cgcaagagac
    36241 tacctagttc cttcacgtgt ccatgctgga catttctatg cgttgccgca atctcctcaa
    36301 ttgtttaaac aattgctgat gaatgctgga ttcgaccgtt attatcaaat cgttcgttgt
    36361 ttccgcgatg aagatttacg tggagaccgt cagcctgaat ttacgcaagt agatattgaa
    36421 acaacgttct taacagcaga agaaatccaa acttacacag agggattgat tgcgaaggta
    36481 atgaaagaag ttcgcggtat cgaagtcaca ttgccatttc ctcgtatgac ctatgatgaa
    36541 gcaatggctc gctacggcag cgacaaaccg gatacacgtt ttgatatgga attgatcgat
    36601 ctaagcgata cagtcaaaga agtagaattc aaagtcttcc aaatggctct tgaaaacggc
    36661 ggtgtcgtga aagcattgaa tgctaaaggc gctgcagatc gctactcacg aaaagacatg
    36721 gatcagttag gtcaatatgt aggacaattc ggagctaagg ggcttgcatg gctgaaagta
    36781 gaagaagacg gattgaaagg tccaattgct aaattcatgg gagaagcaac agaagcaatc
    36841 attaaagcaa cagatgcaaa accaggtgac ttgctgatgt ttggcgcaga taaaagtgaa
    36901 atcgtcgctg ctgcattagg tgctatccgg acacgtttag gaaaagaatt aggactaatt
    36961 gatgaatcga aattcaattt cttatgggta actgattggc cacaatttga attttcagaa
    37021 gaagaaggtc gatatgtctc tgctcaccat ccattcacga tgccaaaaga agaagatatc
    37081 ccgttattgg ctgaagaccc tgcaaaagtc tatgcagaag cttatgatat cgtcttgaac
    37141 ggttatgaac taggcggagg ttctatccgt attcatactc gtgagcttca agaaaaagta
    37201 ctagaaactc taggctttac aaaagaacaa atggaagaac aatttggttt cttgttaaca
    37261 gctttagatt acggattccc tccacatggc ggtattgcat taggattgga ccgttttgca
    37321 atgttattag ccggagaaga taatattcgt gaagtcattg cctttcctaa aaacggaaaa
    37381 gcagcagatg tgatgagtga tgcaccaagt atggtttcgc ctttacaatt gttcgaattg
    37441 aatattgatg taacgaatat agaagaatag ccgataaaaa ggtaaaaagg atttgtcgaa
    37501 tacgcttctt ttgacaacct tgatttaatt gaaaaacact ttataatgaa gccataacat
    37561 ataaagcggg gcttttccat gaaaaaaaca ttcgaaaatt gggcttatca tgattacacg
    37621 accaagcttt cggtatctat cgtttatgcg ttgatcgcat cagtcgcatt gaatttcttt
    37681 tatcagccag gacatattta ttcaagtggt attactggtc tagctcagat tttgacgact
    37741 ttgtcaaaat cactgatagg attcgaagta ccggtatcaa caactttgta tttactgaat
    37801 atacctttgt ttgtattggc gtggttaaaa atcgggaaga agtttacaat tttcactttt
    37861 atcacagtca ctttgacctc cctttgtatc catattttac cggaaacagt cctatctacc
    37921 gatccaatca tctgtgcgtt gtttgggggg gctgtgatgg gactaggtat tgggctagcg
    37981 ttgagaaacg gcctatcatc gggcggtcta gatatcgtga gtatcacgat tcgtaaaaaa
    38041 acaggaaagt cgattggttc tatttcgatc tacttcaatg cattgatcgt ttttgttgcc
    38101 ggaattctat ttggctggca atatatgttt tacagtacgt tgtctatttt cgtcagtgga
    38161 aaagtaactg atgtcgttta tactaaacaa aagaagatgc aggtaatgat cgtaacgaaa
    38221 catccagaaa aagtagtaaa tgaaatccaa aactgtatgc gtagagggat cacgatcatc
    38281 aatgaagcag aaggtgctta taagcatgat aagcagacag tattgatgac gatcgttaca
    38341 cgatacgaac tgcctctact tagagaagca atgaaagctt ctgatccaca agcttttgta
    38401 agtattgcag aaaacgtcca gatacttggt cgtttctatg aggatggcat ttagatcgag
    38461 tttgacaaaa attcatgaaa tactgctgta gacagacgtt cgtttgtctg cagttttttt
    38521 ctaccatagt agaatcagca aagaaaaact gcttttttat ttgaaatttt cttaaagttg
    38581 aaaaaaagca tgtttatttt tttaaattta tgggatgata gaaaaaaata gtagagaaag
    38641 gacacgattc tacttgctat tttcacttaa tacccgtata ttacgaatga aaaaagttta
    38701 tgaaaagttg gaatattgaa tggaaaaaca aaaaaggtta aaaaacagcc gctacatcac
    38761 tgggtttgat gggctccgtt cattagctgt ggtgggtgtt atcttctatc accttctgcc
    38821 aactagtatg aaaggcggct atttgggcgt tccgattttt ttcgtcgtat ctggttattt
    38881 gatcacggat ctgctgcggc aggaatggga acaaaatgga aaaatcaata tttggcaatt
    38941 ttatgtcaga agaatgaaga gactctatcc ggcacttgtt tttatgcttg tgacagcttc
    39001 ggcttatatc actttgtttc agcgagggct gttaaacaat cttcgaggag ttgtggtcag
    39061 cagcttactg tatgtcaaca actggtggca aatcaataac ggtttatctt atttcgatcg
    39121 atttgctaat gagtcaccgt ttacgcatat ctggtcttta gcagtagaag gtcaaaacta
    39181 tttggtttgg ccgatcttgt ttgtattgtt gatggtattt gttcgtaaaa gaaaatggat
    39241 cttttatact gtgttaggta catccttcct ctccgccatc ttgatgatga tcttgtatac
    39301 accaggaggc gatccaaccc gtgtttatta cggtaccgac actcgtctgt tttctatatg
    39361 gttgggaagt gcgcttgcgt ttgtttggcc aagtacgcgt ttaaagaaaa acatcccgaa
    39421 acaagcgaaa aatattctga atatcgctgg aggaatctcg cttttggcac tgattctttg
    39481 cttcttcttt ttagatgatc attacaattt catctattac ggaggaatgc tcctagtgag
    39541 tctgttatgc gtagtgctag ttgcagtgac agcacacccc ggagcaagtt tgaatcgatg
    39601 gctgacaaat ccagtattta cttggattgg caaacgaagc tatggcattt atctgtatca
    39661 gtttccagtt atgatttttt atgaagcaaa ggtgactgat attgccgatc atgttttgtt
    39721 gcatacttta gtggaaatca cattgattct aatcatcagt gaactttctt atcgatatgt
    39781 ggagcgtccg ctagcacgtt ttgattatgg tcgtacattc caagtgatga aaggatggtt
    39841 cagcaaaccg atcatcagta agagcaaacc atgggttgtt ccaggcacat tacttgtagc
    39901 aattgcactc gttggtttcg ttacggcacc gaaaaacgct gtaacagccg atcaaaaaca
    39961 actgcaggca aaaatcgctg aaagtaaaaa attagcagaa gaaaccagaa acaattcaga
    40021 aaacaccact agttcggttg accaagcgat ccttgataag tacgagttgt ccaaggcaca
    40081 agggaataag gcacaaaaat tagaactaac ggcatttggt gattcagtta tgttggatgc
    40141 tgcaacggat ctacaagaac tttatccaaa agtagtggta gatggagatg tcggacgaca
    40201 attatatgcc agtgtaccgt acattgaaaa attaaaggaa caagatttgt tgaaagacac
    40261 tgttctgatc ggactaggaa cgaatggctc attctcggaa gctcagtttg atagtgtgat
    40321 ggatgcaatc ggtgatcgaa aagtatactg ggtcaatgtc cgagtaccga cgaagcgctg
    40381 gcaaaatgac gtgaatgcga tgcttgaaca gatgacgaaa aaatacgaca atatgacgct
    40441 gattgattgg tacagctaca gtaatgacca tagtgactgg ttttatgatg atcaagtcca
    40501 tccaaatcct gaagggatga cacattacat tcacttagtc agcgaagcct tgctaggagg
    40561 aagttcaagt acgaacagca gtgagacaaa cgtgacagaa tgatagctac aaaaaacttc
    40621 aggcaaaaac atttgttttt gcctgaagtt tttttatatt taccgtgttt ttaacattca
    40681 tatagattca ggaagcaaaa cggttgagtg tgtctgtaaa taacgttctt tttgttttcg
    40741 ggtgagtttt ttgaatgcag cttctgcttt tgttgcttcg cttcttgaag aaaatacttc
    40801 tgcatggatc atcttaacag gtcttctttt ttgaagacga gtatatttag caccagtacc
    40861 attgttatgt tcctctaatc gtctagaaag atcggttgta tatccaccat aaaatgtctg
    40921 atctttacag aataatacgt aaaaataatg ttcatttgcc atagagcatc tcacgaatct
    40981 ctgcagtgta ttctccatcc atattgtaag tgatcaatgg tggagcgact ttgaatccgt
    41041 ctaatttgcc ttctttgatt ccttcaacta acaaaatatt tgcctctttt ccaggttttg
    41101 ggtaaacaaa ttggatcctt ttaggtgcaa ttcgatagtc ttccatgacc tgaatgattt
    41161 ctaagaaccg atcaggacga tggaccatag taaagcgtcc atttgttttt agtagcttac
    41221 ttgaaatatc gatcacttct tctaacgtcg tatgaatctc atgacgggcg attgccaggt
    41281 gtttattagg gttcgtttta tttgttggca gccctttgaa ataaggtgga ttacagacga
    41341 ctaagtcaca agaattatgt cgaacgacag ataagctgtc tttcagatcg atggtgtgga
    41401 cagtcatttg ttcatttaaa tggttaagcg cgatactacg tcttgccatg tctgctagac
    41461 gaggttgaag ttcgatagcg tcgatagccg cctgggttcg tttacttaaa aaaagtccaa
    41521 ctgcgccatt tcctgcacaa aggtcaacaa tcttcccttt tttaggtaca cgggaaaagt
    41581 gagcaagcag cacagcatct aaagaaaaag aaaacacttt ggaactttgg ataatcttga
    41641 tatcttctgc gtataattgg tcgattcgtt cgtcttcgtg aagcatggat tccctccaaa
    41701 atgaaaaaaa tagtcgtctg tattatacta tttttattaa aaagaaccaa ggtggcttgt
    41761 caaacctcta ttttttatgc atactatgac cataagaagg gaaaggaaga gactatgttt
    41821 tatcgattta tgcgcggggt agttcgagta ctattagcct tagttaatgg aaatgcccgc
    41881 tatgagaata aagaagtttt accgcaagat gagaattaca tactagttgc tccccatcgt
    41941 acttggtggg acccattata tctggcggta gctgcagcac ccaaggaatt cagctttatg
    42001 gcaaaagaag aactgttcaa aaatccagtt ctgcgattta tattaactcg atccaatgct
    42061 tttccagtta aaagagaaaa tccaggacca agcgtcatta aaacacctgt aaagattttg
    42121 aaatcgacaa atttgagttt gatcatgttt cccagtggga caagacatgc tacagaactt
    42181 aaaggcggaa tggcattgat ttccagaatg tctaaagcga aaatcgtgcc agctgtatat
    42241 caaggtccgt tgacacttgg ggatctgttc aagcgtcaga gagtgacagt gcgtttcggc
    42301 gaaccaatcg acttatcaga tatcaagaaa acggataaag aaggtatggc ggagatcgaa
    42361 cgacgaacac aagcagcttt tgacaaattg gacaaagaag tgaaccctga tttcaaatat
    42421 gaagtcaaaa aaaaataaaa tgataagttt atgcgtgggc ggtcttttga atctactgtc
    42481 ctgttcagtt cagacttttt caatgaaaac gagggagaga cgtcaagtct gccctctttt
    42541 tgcgttccaa tatgtaaaat agaagacagg tgtcatgtaa tggatcaaag tgttataata
    42601 attagaagga cattttttga ggtgattgaa tgcgtttttt acatacagcc gattggcata
    42661 tcggaaaaaa actacaaggt tatgacttga ttgaagacca gcgtcatgta ctatcttcta
    42721 tactagatat cgcgaaagaa gaaaaagtag aggcaatcgt gattgccgga gacttatatg
    42781 ataggagtgt cccagctgtt gatgccgtag aattatacaa tgaattgatg gtcgattgga
    42841 atttaaagga aaagatcccg atttttgcta tatcaggaaa tcacgatagc agtacgagac
    42901 tttcagcggg tacgccatgg ttcagttatt cgaactattt tttgcgaaca cagctagcag
    42961 aagcatttga accagtcgag tttaaaaacg tacaattttt ctttcttcct tactttgaac
    43021 caattgcagc gagattatat tttgaagatg acaatatccg aacgatcaag caagcggtag
    43081 ccaaggtgat agataaaatg caggagctat ttgattcaga aaaaaaacaa gtattgatta
    43141 gccatttttt tgtaatggga agtgtaaaat ctgattcgga aacgaaaatc gaagtaggcg
    43201 ggttggattc gatccctggt gaactactta caccgtttga ttatgtagca ctaggacatt
    43261 tgcataatca agctgctttg agacaggata atgctcgcta tagcggttcc ccattgaaat
    43321 tctctttatc tgagatcaac caaaaaaaag gcgtatggat cgttgacctg acaacaggcc
    43381 tggagttgaa ttttagggag atcgaacccc tgcatgatat tgaagaaata acagcgagtt
    43441 tcaaagaatt gctggatcct gccttttatc agtccgtcaa tcgggagaat tacatctata
    43501 tctatctcac tgatagaacg atcatcccga atatgatgaa ccagcttcgg caaatatatc
    43561 ctcgtatttt ggggattgaa cgggcaaacg gtcgagcaga acaaacaaag cgcaaacgaa
    43621 aagtactaga aatgaaagat cctcaaaaat tagcagaagc attttttagt gaaatgacgg
    43681 aagagcagct aactgcaaac caagaaaaat ggctcggaga aacgttgcaa gaaatcatgg
    43741 aaatggaatg atcaagaatg aaaccgaaaa aattacaact taaaaatttt ggtcctttta
    43801 tcaatgagac cgtagatttt tcacgtttga cagaagcacc gctttttttg atcagcggga
    43861 agacaggagc tggcaaaacg acgatttttg atgggatcac cttcgcactt tttggtgaaa
    43921 cttccggaag acttcgctct ggaaaagaga tgcgttcttt gtttgctaca ccagacgaag
    43981 aaacaagcgt gactttttct tttgaacatc agcagatgag ctatgaaatc gaacgaaaac
    44041 cagaacaagt tttagcaaaa cgaaaaggaa acggaacaaa aaaacaggca gccaaagtaa
    44101 cgttgaccat ttttgatggg aaaggaaaag aaataaaaca aatcacaaaa cgtacagaag
    44161 cagatcagtt gatcagagaa ctgatgaatc tcgatgcgaa acagttttct caaatcgttt
    44221 tactacccca aggagaattc cgtaattttt tggtttcatc aagcagtgat aaagagactg
    44281 ttttacgtca tttattcggt actcaatttt tccaacagtt caatgagcga ttaaaagaaa
    44341 aagcaaaaca acagcaacaa aaattggatc atttagagca agagctgcat cttctgcaaa
    44401 aaaggtttgt tttagtcaaa gaagaggaac cagcagcagg gtttgaagcc gttttagccc
    44461 aatggacaaa ccatcaggca atactcgaaa aacaaatcac ggaagaaaaa gaagctctaa
    44521 cggaacttca ggaaaaacaa aagcaactag aaaattccta ctatcgtttt gaaagcttga
    44581 aaaaaagtta tgaagaaaaa acgatattaa taaacaagca aaaggaatta gctgctcaaa
    44641 aagacgaaat agaggaaaag aaacgatgga ttgggtatta tgaatttacg caacgattag
    44701 tcgaaccaat ccagcgccta caagaaataa aagttgaaaa aatagaactg tcacaaaaaa
    44761 gtagtgagca aacaaagaaa ctagcgatga ctacgcagaa ttatgaagag tggcaaaaaa
    44821 atgaagcaaa ccgcgtacgc cgacaaacaa aaatcaaaga agctgctcaa aaactgcaaa
    44881 acaaacagat gatggtgcca attgtcgaat ccttacgtag taaaaaacaa gacgcagaac
    44941 aattaacaat ttcattggat aaagaagaat tgaatgccga gaagctgtat caaaatctcg
    45001 ctatctatca ggaaagaact cgaattcttc aagaaaattt actggatcag gaaaaactgc
    45061 aagaagatcg tcttgctttt ggacagttaa aatatctaga tgaaaagcag caagaaatag
    45121 aaaaacaaaa agcaaaagac actgggtcgc tcgcacagat aagagaagaa ctcaaaaaag
    45181 caactgaaga aataacaaaa aatagtcagc tattgaaaga acagacagaa caagcagaac
    45241 agctgaaaag ccaatgggca aagcagcaaa ttgctcgtct tcagcttttt ttgcgtccag
    45301 gggagccatg tcttgtttgt ggatcaacgg aacaccccat acaacaatta aaacacgaac
    45361 agccaaataa ggaagatatc ttaaaagtag aacaacaact ggcagaagct gagtctgctg
    45421 tagaaagaac tcagcaaatc attgccaaaa gtgaagcaca tgaaaataat cttcaacaac
    45481 aattatcaga tttagaacaa aaaatcatgg caacgaatat acaattggct gaacaagaag
    45541 aacacttgca acaggctttg aaaaaggcat ttcctgagat aacagaacaa aaaacaaaaa
    45601 caactcttgt agtaatcgaa caatctttag atagacgaaa aagcaagatg gccaatgcaa
    45661 gaatagagct ggaagaaata agacaacaag cagaaaagct cgaacaacaa ttagctgtct
    45721 acaaacagca gcagacagaa aaaaataatc agctccagca actgcaagga gaaatcaaaa
    45781 gtttagaaca acaattgcaa gatacggata gtaactgttt agaagaggag atacgacgac
    45841 ttgaaaaaca gttagaagaa gactcgaatc aactagctgt tgagcagaaa gcaggagtgc
    45901 aattgttgca agatcttcat acgcttcgga cacagcttga tcttcaacat gaacaaatag
    45961 aaaaaataga tgaaaaaata gaaaaggaac aagaaagaat agctaaaaag ttagaaagcc
    46021 agtcgtattt ttcaacaatt gaagaaatct gtgcatttgc acaacagcaa gcacaatata
    46081 aagaaaacca acgtacagtt acttcttatc atgaagagca attagtgaca gctgaccggc
    46141 tgcgtcaatt agaaaatatc cagctaactg agacattccc agatacagaa gagatcagcc
    46201 aacagttaga agaaataaga cgtatgatcg aaataaagca agaaaagata tacggtttac
    46261 aagaacaaaa aattagtaat caaaaaatct acgatgaatg tctttcgatt tatcacacaa
    46321 gccagacgaa attagatgag cttacgcaat tacagcagct ttcgcaaaca ttcaacggag
    46381 aaaatcctaa gaagactagt ttggaacggt atgtgcttca ggtttatctt caagaagtgt
    46441 tacaagtagc aaatgatcat ctccaacgtt taacgaaaaa tcgttatcaa tttgagctag
    46501 cggataccat tggcagttat cgagggaaaa ccggattaga aatcaatatc tatgatgatc
    46561 aggcaggtat gtctcgaagt gcgcataccc tgtctggcgg tgagagtttt attgcagcac
    46621 tttctttagc attagctctt gccgatgtga tccagacaca agctggtggt gtagcaatcg
    46681 atgcattgtt tattgatgaa ggatttgggt cattagatga agaagctttg gaaatggcaa
    46741 tggaagcttt agaaacgatc gaaagtgaag gaagaatgat cggcatcatt agtcatgtaa
    46801 gagaattgaa agaacgagta ttacaacaaa taagaattga tacagaaggt agcggacaaa
    46861 gcaaaatacg ctatcaactt ggataaaaag ggagattggt aactatgaaa tggagtattc
    46921 cagaaaaagt cattgcacgt ggacgtcaat acttgcaaga aggccgagtg ttatcggttg
    46981 tccctgacca agaagaaaac gtgtggcatg cagaagtctt gggcagtgaa ttgtatatgg
    47041 ttgatttgga tggtacagca aaagagatag attactgcca atgtccctat tgggaggaac
    47101 ataaatattg taagcataca gttgctgtgg aactttattt acggtcgaaa aatctatctc
    47161 gtatcatgaa aaaagaccag ccaatgctag aaccggtaaa agcaacaagt gagggagaaa
    47221 tcattgcaaa aggtttttct cgtctaaaaa cacaaaacaa tcctcaagcg gtgcaacctt
    47281 tgattattga ataccaagta gaaacgattg agactaatca gtatcaccca gagctttcta
    47341 ttttggcact ttacttgcgt atcggttcat tggcatcacc gaaaaaaaca tatatcgtaa
    47401 aaaacatcta tgatttttta caagcttaca cagaaaaaga aacttacact gttaataaac
    47461 agtaccagtt tcgtctagat aaacgggcgt ttcaaaaaga agatcaaaaa atactcacgc
    47521 atcttgcagg aagtgctcag acgcatcaat tattaggtac gaacggctta caagtcaaag
    47581 gaaaactaga taaaagatat ctgcttctcc cagtagaaca agcccgcttc ctgttagagc
    47641 agatgaatca gactacacgt ttgcatctgc agataaatga acaaaacgat caaggaatac
    47701 aattttccga tcaaaacaaa cctttatcat tcaaagtgga aaaatttgga gaagattatt
    47761 tgctgcaagc attgaatgat tttgattttt tctttatgca ttatcagtgg ggagttatcc
    47821 aagggacatt ttattctctg acgaaatatc aacaaacggt ttatcaaact tggaaacaat
    47881 tgattcgccg attggagaaa ccagaagtag tgttcaagaa aaaagaatta ccgtctttgt
    47941 ttaaagagat tatccctctg ctgtcagaga tcggcgacgt agctattgcc cctgcagtag
    48001 aagaggaagt agcagatatc cccgtcgaat ttattttctt tttccgaaaa gcaaaaggaa
    48061 aaatccaagc tcgagtcgat ttcgtctatg gcgaagtcat ctattcgact gacccgaagc
    48121 atgaggtctc gacagaacag gcttttcagg tgttacgtga cacagtagaa gagaaacgag
    48181 tacttgatct attcagaatg tatcgctacc agaaaaatga aactggttat gaacgtccat
    48241 tgccaagcgg ggaagaacta tatgcctttt tccgcacaga actatctgtc ttccgtcagt
    48301 ttggtgaagt cagacttgga agaaaattgc gagagttgta tttagatgcg cataaacatc
    48361 aaccgaaaat cgaagtaacc gagaccggtt catggctcga tattcgattc gatgtgacag
    48421 ggatagagga gcaagaaatc gatcatgtcc tgcaaagctt gctgagaaat gatgcttttt
    48481 atacattaga aaatggtcag gttctttctt ttgattcaga agaatttcaa caaacaagcc
    48541 aagtgttgca acaattacgg gaatcgatac gtacagaaga aggaacgatc catgtaccta
    48601 aaaatcaagg attgatcatt caaaatcaat tggaaaaaag caatgctact tttagtgaat
    48661 cgttccaaac gatggtacaa gatctcattc atccagaaag ataccaggca cagcttccca
    48721 aaggattgaa tgcaacgatg agagattatc aaaagcaagg ttttcgatgg ttgaagatgt
    48781 taggtcatta tcagtttggc ggtattttag ccgatgaaat gggacttggg aaaacactac
    48841 agacaattgc ctttttactt tcggaaaaag aagaacggaa aagcttctct gctttgatcg
    48901 tagcgcctgc gagcttaatt tacaactggc aggcagaggt gagaaaattc gccccttctt
    48961 taagtatcca agtaatcaat gggaataaaa aagaacgtga agaattactg gctaaagaca
    49021 cagatatccg agtgacttct tatgccagct taagacagga tctagctaat tatcagtcgc
    49081 aaaaaattga ttatttgatt cttgatgagg cacaaatggt caaaaatagc agtacgaaaa
    49141 ctgcacaagc attgagagaa ttagctgtac ctcagcggtt tgcactgagt gggacaccaa
    49201 tcgagaataa tttagaagaa ttgtggtcat tgtttgcaac gattatgcca ggatttttcc
    49261 cgactaaaac aaaattcagg gaacttgccg cagaagaaat tgcgcaaatg attcgaccgt
    49321 ttattttacg gcgagataaa caaactgtgt tgaaagactt gccggaaaaa acagagatga
    49381 atctatattc tgcacttaca gaagaacaaa aaactgttta tttagcttac ttgcgtcaaa
    49441 tgcgtgaaga aatcacatca atggattctg aagcattcaa gaaaaaccgg atcgggatac
    49501 ttgctggtct gacaagactg cgacaaattt gctgcgatcc tcgtctgttt attgaagatt
    49561 accaaggcgg ttccggcaag ctcgaacaag taaaagatct actggtagct gccaaagaga
    49621 acaaacgaag agttttgctg ttttctcaat ttacagggat gctgactatc ttgcaagaag
    49681 agttagccga gctagggatc tcgacatttt atttaagagg cagtacgaaa ccacaagatc
    49741 ggttaagtat ggtagacgca tttaatgctg gcgaaaaaga tgtttttctg atttccttaa
    49801 aggctggggg aactggattg aatctcacgg gagcagatac ggtcatcttg tatgaccttt
    49861 ggtggaatcc tgccattgaa gaacaagctg ctggacgtgc acatcggatc ggacaaaaaa
    49921 atgtggtaga agtgtggcgt atgatctctg aaggaacgat tgaggaacgc atggattcac
    49981 tccagcaaga aaaacgcgaa ctgttccaaa aagtgatcca aggtaatgaa gaacagttga
    50041 cgaaactgac tgaagaagac atccgcatga ttttaagtgt aggagaaatc gaggagtaag
    50101 agactcgaaa aagcgttcag taatgggtaa gaaagccttt ctaaagaagc gattgtttat
    50161 ttgatttaaa aaatagcaag gaggctggga ttccatttta tcccagcttc cttgctatca
    50221 tttttttatt tctattttta aaggttatac aaagattgtc ttcattgtct ctaacagctg
    50281 atcaaaagaa gaaatctcga tatcgcaaat tggagtctgt tcaattttgc gttctcgatg
    50341 gttgaaccaa agcgcttgcc agttagcact ttttgcacct agaacatcat tgtcgtaatt
    50401 atcgccaacg tataaggtct cttctggaag catatgaaat tctttctcag ctagctgaaa
    50461 aatctctttt tctggttttt ggaaacctgt tgcttgagaa atgagcatat tgcgggaagg
    50521 aatccaattg ttcaattgaa gttgcttcag tttttttgtc tgatggtcag tcggcccatt
    50581 tgtgataatt cctaatggga cattcttttc cttcaagaaa tcgagggttt ttttgacctc
    50641 ctcatgcaag cagatattat caagttcttc ctcgtaaatt ttttgaaata agaggccatc
    50701 ttcttccgta atatgagggt agtctaaatc ttttagtgat tggctgatcc gatgtgctcg
    50761 catatattct agtgtccaat ctcctgccat tacttttggg aagttttcat cactatgatg
    50821 gcggaaccgg atatatagtg gatgcatatc tgcatctgaa acaagcggaa cgactctttt
    50881 gaccgcgttt cgaaatggct gctgctgatc atacatcgta tcatcaacat caaacacaac
    50941 tgatttaatc aaaattttac acgcctttcg gttttcattg attttttcac aaatccttga
    51001 ctattgtaac agaaaaaatt gtctaaaaaa gctgataaat aaaggttttt cttactatat
    51061 ttctatttcc ttgacagaat aaggttttta tatttcagtt tttttcacta aaaatcgtga
    51121 aacacgtcaa taagtttgat gaacaaatta atcattatgg tagaagtcga ttgatttatt
    51181 ttaatgcaat ctctgtggaa ttttcgaaag atttattttt ttaatacagt ctcaatataa
    51241 ctattcaaac ataaaataac gttatttgat ttatttgttt atagtaaatt ttatttagta
    51301 atcattgctt taaaatacta cgcttatata acagagaaaa taagcgttat aataaagaac
    51361 agtattattt aaaatgacag aaaacattac tattaaagaa ataaaaaaca taaaaaatct
    51421 taagaaatcg gtttcattta gcttttgaga taaaataata gtacagtttt atacgctgtt
    51481 tcatatgttt agaaaatttg aattagattt gaaaactcta atttttaaat gataaaaaaa
    51541 gcgagaatgc tggttttact gtatattaac cttagataac tttcattttt ttaaaaaaat
    51601 tatgaaaaaa aggtagctat ttaaaagttg attatgtata atagaaacag atgggagatt
    51661 attatgatct tctgttacaa aaaagtgatt cttatcacga aaaggagtgg tgcacgttgt
    51721 taacattata tacttcccca agttgtactt cctgcagaaa ggctcgtgcg tggttgcaag
    51781 aacatcagat tccatttgtt gaacgtaata ttttttcaga accattgaac agttctgaat
    51841 taaaagcgat tttacaaatg acagaagacg gaacagaaga aatcatctcg acacgttcca
    51901 aagtattcca aaaactgaac atggatttag atgatctgcc gttacaagaa ttattggaac
    51961 ttgtgcaaaa caatcctgga ttgcttcgcc gtccaatcat gattgatgat aaacgtctgc
    52021 aagttggttt taatgaagat gaaattcgtc ggttcctacc gcgcgatgta cgacaattag
    52081 aattacgtca agcacaattg atggctggat tatagaagga taatacgaaa cttggaaaag
    52141 actattacaa agatagtttt tccaagtttt tttgttttta caaaaagtat tcttgattag
    52201 atgtataaaa aggtagtaga agatgatgat tgcacgaaaa atagagaggg aaaattagct
    52261 gaaaagtcgg ctatatagta ttcgtctgcc aaaaatgtta tagttaaata agatagcttg
    52321 aaaggttgaa aaatccgctg gttttattaa attcgcttaa cagcgagatt ttttttcttt
    52381 acatttaagc tgatttcgct acaatgaatc tatattaaac attgtggcaa agtgaggtgt
    52441 agcaaaatgg aaatggaaca tattaatgaa aatacgatcc gtgtactaat tggtcatgaa
    52501 gatttagagg aacggggcgt ttcatttttg gatttacttg gcaatcacaa agaaatcgaa
    52561 aatttctttt atagtatttt ggaagaagtt gatatcgaag aggagttccg aggaagcgaa
    52621 gctgtcactt tccaagtttt gccaaaagga gacggattag aactgtttat cagtaaaaat
    52681 cttccggatg aagaaatttc tcaatcagaa gattctactg ttgattcaga agatgtgagt
    52741 gactttcttc gtcagcagat tattggcagc gatccggagg aagaattatc tggaagcgcc
    52801 ctagaagaac aggaaagaca agcaactttt gcttttgaag attttgaaca agtgatccaa
    52861 cttgcaacag atgtcgaact agagtctgct tggactgatt tgtatttatt cgaagagcgc
    52921 tattacctag tcgttcactt ctggatggaa aatctcaacc aagcggatgt agagaatcag
    52981 attgctcgaa ttttggagtt tgctgaaaag agcgatcgta caccagaaac attgaatgaa
    53041 tatggacaat gtctgatggc gcgtaatgct atcgagcgaa cgaaatttta ttttgtataa
    53101 atcagcatta tagtaagaga ctgtgacaaa aattgtgtcg cagtcttttt ttcgtataaa
    53161 tgtgttcaaa agcttgcatc tgcggtaaca agcccaacca aacgaaaaat attgggaact
    53221 atttttgttt agctgttctt attacttgat gcagaatagc tttttcacgc cctctttttt
    53281 cgtgtaaata aattcttaga cgtaaataag ttcagaggga gaaaaatgct gcttgcaatg
    53341 aatgaatata ataagtgctg ttttgctaaa catgcagaaa aaggaaagaa atattattgt
    53401 cgtggctgtc gaaaaagagt gattttgaaa aagggaaaga aaaaatgtgc tcattttgcc
    53461 catcaaaaat cagataactg ttcggtcttt agtgaaagag aatcaaaaga gcacttacag
    53521 ttaaaggaat gcttcatgga ttggctggga cagtcagtag aaccagtatt tttggaagcc
    53581 tatttgccga ggttgcggca aaggccggat attttgttgg cgaatctagc aatagaaatc
    53641 caatgttcta gactgtctca ccagagattt attgaaagaa cccaaaatta tttgaataac
    53701 agttaccaag tatggtggat acttggtaat tcttttcttg gacaatcgca attctcattg
    53761 atagaaaaaa gttgctgcta ctataataga aaaagaggag tgcattgctg gaaagctgat
    53821 ctcaagaaac aaaagcttta tttgtatcat catattacag agactgtatc agggcatatt
    53881 tccttttgca gtagctgctg gactttttct agtagggacc ttaaagaaat attcactagt
    53941 aacgaaatta aaatgaacca aatgaaaaaa atagaaaaag tgagtgaaga tgggaagaaa
    54001 tggctgacac gccaacttat tcacaagcaa aagaatacag tatccataca agaacaatgc
    54061 tacttgcgtc ataaacatct tttgcatcta tcatcgtgga tctatcaaaa aagtcggttt
    54121 ttcttttatt tacaagaaca ggtttttctt tatcgaatgt tgtacgaaga agccttggaa
    54181 aaccagaaag ttcctgattt caactcatgg ctttgccaaa taaaagaaca taaaagggaa
    54241 tggctttttc ctatgattga tgaagagatg gtctatcagc agttttttaa cgaatgcata
    54301 cacttgtctt ctttgaaaaa atagtaagta taaaaatagg ctaggaatcg gttttctatg
    54361 taaaacaaga tttagttatg ctaaaattga tggaagcaga cacatgaatg agtagaaaag
    54421 ttagatgctg tgacatgtgg atgttagact atgatctatt ttgatagata gagctaaggg
    54481 aagaaaagga gtggatttca tggaagtaaa gcagttgcca aaacgagaag aattgcctga
    54541 aaatttaact tgggacttga ccaagatctt ttcaagcgac caagagtttg atgagaaata
    54601 tttggaatta tcagaagagt taaaacaatc tgaaaaacac aaaggaacac ttgatcaagg
    54661 cgcttctcaa tttttaaatg cgattgaatt cgtattgagg gtttatcgcc aaactgaagt
    54721 catttatgta tatgcgcacc ttaaaaacga tcaagacact ggaaatacag attaccaagc
    54781 gctttatgca agagcaagca gtctgttttc gaaagttagt gaagccgttt cctggtttga
    54841 accagaaata ttgcaattgt cagatgacca gatttggcaa tatttcaaag aagaaccaaa
    54901 attggaagtc tatcgccatt atatccagca aatagtagat aatcgagccc atgtcttatc
    54961 tgctgagcag gaatctcttc ttgctggagc aggtgaaatc tttgatgctt caagtgatac
    55021 atttgctgtt ttgaataatg cagatctagt ttttccaacg attgaaggag aaaatggtga
    55081 aatagtccaa ttatctcatg gcgtgtatgg tcagttgcta gaaagcacgg atcgaagggt
    55141 gcgcgaagca gcatttaagg ggttgtacag tgtttacgaa caatttagaa atacatttgc
    55201 ttctacttta ggcacacata taaaaggaca taattttaaa gcgaaagtcc gtaattacag
    55261 ctctgccaga gaagcgtctt tgagcaataa tcatattcct gaaagtgtat acgatacttt
    55321 ggtagacgtg gtaaacaagc atttgccttt gttacatcga tacatggaat tacggaaacg
    55381 tttattagaa gtggaaaaac tgcacatgta tgatctttat acaccggtct taggggaagc
    55441 tccaattacc tttacgtacg aagaagcaaa agaaaaagct ttagaagcac tgaaaccaat
    55501 gggtgaagaa tacatggcca tcgtagaaaa agcattctct gaacgttgga tcgatgttgt
    55561 cgaaaataaa gggaaacgaa gcggtgctta ttcttcggga agctatgaca caaatccata
    55621 tattttattg aattggcatg atacgctgga tcagctattt acgcttgtcc acgaaatggg
    55681 acatagtgtt catagttatt tcactcgttc gaaccagcct tatgtgtacg gcgactactc
    55741 catcttttta gcagaaattg cttcgacaac gaatgaaaat atcctaacgg agtatttatt
    55801 ggaaacagaa aaagaccctc gtgtacgggc ttatgtactc aaccattact tggatgggtt
    55861 taaaggaaca gttttccgcc agacacaatt cgctgaattc gagcatttca tgcatacgga
    55921 agatgaaaaa ggtgtgccat tgactagtga atacctaagt gatagttatg gtaaattgaa
    55981 tgcaaaatat tatggtccag cagtcgaaga agacccagaa attaaattcg aatggtcacg
    56041 gataccgcat ttttattata attactatgt tttccaatac tcgactggct tttctgctgc
    56101 ttcagcactg gcgaagaaaa tactaaacca ggaaccagag gcactagaga actacttggc
    56161 ttacctaaaa gcaggtaaca gcgattatcc tgtggaagta atgaaaaaag caggagttga
    56221 tatgacacag gctgcatata ttgaagatgc aatgtcgatg tttgaacaac gtctaaatga
    56281 attagaagaa ttgatcgatc gtttataagg ctttctattt ttgattttct cgaatagaaa
    56341 cgaaaaaaat acgtcggctc tcttgtaagg gagtcgacgt attttttta
//
LOCUS       DACDYI010000011        48774 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.011, whole genome shotgun sequence.
ACCESSION   DACDYI010000011 DACDYI010000000
VERSION     DACDYI010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.011"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(23..2095)
                     /locus_tag="IWM52_000617"
     CDS             complement(23..2095)
                     /locus_tag="IWM52_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9972894.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(2028..2405)
                     /locus_tag="IWM52_000618"
     CDS             complement(2028..2405)
                     /locus_tag="IWM52_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAP9972895.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(2534..3094)
                     /locus_tag="IWM52_000619"
     CDS             complement(2534..3094)
                     /locus_tag="IWM52_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9972896.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            3244..4536
                     /locus_tag="IWM52_000620"
     CDS             3244..4536
                     /locus_tag="IWM52_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9972897.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(4596..5714)
                     /locus_tag="IWM52_000621"
     CDS             complement(4596..5714)
                     /locus_tag="IWM52_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAP9972898.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(5728..7149)
                     /gene="arcD"
                     /locus_tag="IWM52_000622"
     CDS             complement(5728..7149)
                     /gene="arcD"
                     /locus_tag="IWM52_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAP9972899.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(7395..8516)
                     /gene="mnmA"
                     /locus_tag="IWM52_000623"
     CDS             complement(7395..8516)
                     /gene="mnmA"
                     /locus_tag="IWM52_000623"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAP9972900.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            8920..9519
                     /locus_tag="IWM52_000624"
     CDS             8920..9519
                     /locus_tag="IWM52_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAP9972901.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(9657..10943)
                     /gene="efmA"
                     /locus_tag="IWM52_000625"
     CDS             complement(9657..10943)
                     /gene="efmA"
                     /locus_tag="IWM52_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAP9972902.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11214..11558)
                     /locus_tag="IWM52_000626"
     CDS             complement(11214..11558)
                     /locus_tag="IWM52_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAP9972903.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(11695..12831)
                     /locus_tag="IWM52_000627"
     CDS             complement(11695..12831)
                     /locus_tag="IWM52_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP9972904.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(13020..13994)
                     /locus_tag="IWM52_000628"
     CDS             complement(13020..13994)
                     /locus_tag="IWM52_000628"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP9972905.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(14074..14949)
                     /locus_tag="IWM52_000629"
     CDS             complement(14074..14949)
                     /locus_tag="IWM52_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAP9972906.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            15079..16986
                     /locus_tag="IWM52_000630"
     CDS             15079..16986
                     /locus_tag="IWM52_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAP9972907.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(17527..20805)
                     /locus_tag="IWM52_000631"
     CDS             complement(17527..20805)
                     /locus_tag="IWM52_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAP9972908.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(21076..21621)
                     /locus_tag="IWM52_000632"
     CDS             complement(21076..21621)
                     /locus_tag="IWM52_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9972909.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(21633..22325)
                     /locus_tag="IWM52_000633"
     CDS             complement(21633..22325)
                     /locus_tag="IWM52_000633"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAP9972910.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(22344..22628)
                     /locus_tag="IWM52_000634"
     CDS             complement(22344..22628)
                     /locus_tag="IWM52_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972911.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(22641..23204)
                     /locus_tag="IWM52_000635"
     CDS             complement(22641..23204)
                     /locus_tag="IWM52_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP9972912.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            23432..24094
                     /locus_tag="IWM52_000636"
     CDS             23432..24094
                     /locus_tag="IWM52_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAP9972913.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            24218..25414
                     /locus_tag="IWM52_000637"
     CDS             24218..25414
                     /locus_tag="IWM52_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAP9972914.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(25515..26891)
                     /locus_tag="IWM52_000638"
     CDS             complement(25515..26891)
                     /locus_tag="IWM52_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9972915.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(26928..27794)
                     /locus_tag="IWM52_000639"
     CDS             complement(26928..27794)
                     /locus_tag="IWM52_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAP9972916.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(27883..28908)
                     /locus_tag="IWM52_000640"
     CDS             complement(27883..28908)
                     /locus_tag="IWM52_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9972917.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(28926..29600)
                     /locus_tag="IWM52_000641"
     CDS             complement(28926..29600)
                     /locus_tag="IWM52_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9972918.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(29784..30677)
                     /locus_tag="IWM52_000642"
     CDS             complement(29784..30677)
                     /locus_tag="IWM52_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9972919.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(30751..32448)
                     /locus_tag="IWM52_000643"
     CDS             complement(30751..32448)
                     /locus_tag="IWM52_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9972920.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(32441..33754)
                     /locus_tag="IWM52_000644"
     CDS             complement(32441..33754)
                     /locus_tag="IWM52_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP9972921.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPQAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESSEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMDELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(33754..37791)
                     /locus_tag="IWM52_000645"
     CDS             complement(33754..37791)
                     /locus_tag="IWM52_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP9972922.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFYTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVYETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(38560..39078)
                     /locus_tag="IWM52_000646"
     CDS             complement(38560..39078)
                     /locus_tag="IWM52_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP9972923.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(39080..39697)
                     /locus_tag="IWM52_000647"
     CDS             complement(39080..39697)
                     /locus_tag="IWM52_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP9972924.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(39694..40548)
                     /locus_tag="IWM52_000648"
     CDS             complement(39694..40548)
                     /locus_tag="IWM52_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9972925.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(40545..41456)
                     /locus_tag="IWM52_000649"
     CDS             complement(40545..41456)
                     /locus_tag="IWM52_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9972926.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(41497..42831)
                     /locus_tag="IWM52_000650"
     CDS             complement(41497..42831)
                     /locus_tag="IWM52_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9972927.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTYTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            43039..43869
                     /locus_tag="IWM52_000651"
     CDS             43039..43869
                     /locus_tag="IWM52_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAP9972928.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(44113..44362)
                     /locus_tag="IWM52_000652"
                     /pseudo
     CDS             complement(44113..44362)
                     /locus_tag="IWM52_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(44553..44747)
                     /locus_tag="IWM52_000653"
     CDS             complement(44553..44747)
                     /locus_tag="IWM52_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972929.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSGF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(45252..45437)
                     /locus_tag="IWM52_000654"
     CDS             complement(45252..45437)
                     /locus_tag="IWM52_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972930.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(46286..46627)
                     /locus_tag="IWM52_000655"
     CDS             complement(46286..46627)
                     /locus_tag="IWM52_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP9972931.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(46627..46857)
                     /locus_tag="IWM52_000656"
     CDS             complement(46627..46857)
                     /locus_tag="IWM52_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9972932.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            47144..47482
                     /locus_tag="IWM52_000657"
     CDS             47144..47482
                     /locus_tag="IWM52_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972933.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(47687..48262)
                     /locus_tag="IWM52_000658"
     CDS             complement(47687..48262)
                     /locus_tag="IWM52_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAP9972934.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
BASE COUNT        14165 a        10400 c         8547 g        15662 t
ORIGIN      
        1 tcacaacctc tttcatatct tattacttct tcattgctgc ttttagtgct gctgctaatg
       61 ggctttcttc tggttcttct gcttgtgcat attttttcag caagcgtcgt tcttcatgtt
      121 tggtcatttt cttcttctgc tcttttttat caagcaattt ttctgtcgtt ccatcgtatt
      181 tacaacggaa ataagcaccg tttttacctt cgatgatttc cattttccgg tgacattgcg
      241 ggcatcgatg attagagact tttggatctt ttcttctgcg gtagttgcaa tctgaattgg
      301 tgcagacata gattttgccg tcacgtgtgt ttttctcgcg cagattggcc ccacattctg
      361 ggcatttctt ttgggtaatc gagaaatctt gatattcttg tttgcttgat ttgatctcat
      421 tcaccaagcg ttttgtttct ttttcgattc cattcaagaa ggttcctgct tgtttctttc
      481 ctgcagcaat agcttcaagg tctttttccc agctttctgt caaatctggt gttactaaag
      541 agggatttac caatactaat aattgcttac ctttaggaga tacttgcaat ccttgagggg
      601 tacgttccat cagttcagat ttgattagtt tctcgataat ctctgcacgc gtagctgggg
      661 ttcccagact gtatttttcc atttgaccaa gcaagcttgc ttctgacaat ggtttaggcg
      721 gtgcagatag ttcttttttg atggcgaaat cgggattgat tctcattcct ttttcccatt
      781 tgactgtttc cgtctcagtt tcagtttcgc ctttccatcc tgcttggacg acacgatttt
      841 gccgaaaaat gaatcgttct ttaccaaatt caaccgtaac ttttgtttgg ctgactttat
      901 ggggttgagc aaacagtcct aaaaaacgag agacaatcat ttgatagatt tttcgctctt
      961 cattacttaa tttttcataa cgtgctctgt tttcagttgg caaaagtgca tggtgatcgg
     1021 tcactttact gtcttggaat acttttgtct gttggacgat ggctccgttt ttcaaatact
     1081 gcttgacctc aggagcaaag tcgctgactg cttgcaatcg ttctttcatc gtacttttca
     1141 tgtcatttgt cagatatttg ctgtctgtac gcggataagt aacgatctta tgggtctcat
     1201 acaaactttg tacaagactt aatgttttct ttgcagaata gccatagcgt tgattggctt
     1261 ctctttggat ctctgttaaa tcataaggca atggcgcaga ttccgttttt aatttttctt
     1321 ggacttctgt gacagttcct ttgcttgtac tgatcaattg aacaagtttt tgtgcttctt
     1381 cttgtgtttt taaagcaaat tgattttttt gaagcatttt ggcttgttct tttccagctt
     1441 gcaacgtgat cgtgaaatac gtttgtggac ggaactgctc gattttcttt tcttggtctc
     1501 tcactaatgc tagtgtcggt gtctgcacac ggcctgctga taaattatcc gcgtacttca
     1561 cagttaaggc tctggtcaca ttcaatccta ccagccagtc cgcttttgcc cgggcaagag
     1621 cagattcata caatgcatca tattctttcg caggtcttag ttgcttgaat ccagttttga
     1681 ttgctttatc tgtttgagaa gagatccata accgcttcac tggtttattg aaatgcacat
     1741 actctaagat ccagcgagca acaagttccc cttctcttcc ggcatctgtc gcaatgactg
     1801 cttctgagac atcttttcgc tgagccagct gcttgattgc ttttaattga tgtcctgttt
     1861 taggaagtgg tttgattcct agttttttag ggatcattgg caacgtgtcc atttgccagc
     1921 tctgccattc cttattcaga tcttccggca ttttcaatcc taataaatga ccaagggccc
     1981 aagtaacgat caccttgggt ccttcataat aatttttgtg cttttgatta gcaccaagca
     2041 ccttgcttag gtctttcgca acgcttggtt tttccgctag aattagttgt ttcataaaat
     2101 tcctttctgc tttctactgt gatgggctga tgatttttca acaacgcaag tcccttgtat
     2161 tgctgtaaat catcgtcaca ctcttcacac cagcgctcat ctccgtcatt ccaaaaggcg
     2221 ctgagaaagt tactttttgc attgatatat tcataatttt gatggatttc gttccattgt
     2281 ttttcgtacg cttgtcgtgc ttcttcgaaa gactcgaatg tttcttcttc ttcaatatct
     2341 tcttcccagt cttcaaaaaa ccaccaaggc tcattgtccc cgaacatggt aattacttga
     2401 tacatactcg tgctccttct ttcagctttt ctagactcat cttagtcact tttcaagaaa
     2461 gttgcaagaa ttttctctta taaaagggtc atttaatgaa catcttaaaa ttgttacaaa
     2521 tgaatctgat tgtttaaggc aaagtagcga taaagttatt gatcatccaa aaatagctgt
     2581 cttctggatc gattgttggt tcttttgtaa agtatcctaa aaaacgtaag gaaagataac
     2641 cgtggatcat actacgaagt tccctgcttc gatgaacttt ttcttctttc gttaattgaa
     2701 aagcctcaaa tatctgcaaa atgatctgat tcgtttcatg gattccatct aataaaacag
     2761 cttcattggt atgcggtaca ctgatcagta actcatacac tgcttgattt gaaaaagcaa
     2821 aggatcgata agtttctgca tagatctgca gtgcttcctg accacttttt cccacaagag
     2881 cccgtcgcag ctggtcatta agttcctgca gaagtttcgc tgttagcgct gttttgacat
     2941 cacgaatatt tttgaaatgg ttatacagcg agggagcctt gatatccaag cgtttggcta
     3001 aatgctgaaa ggttatttgc tgaacatcca tctcttctgc tagttctcga aagcagtcga
     3061 tgattttctc ttgagacaga tttctttttt tcatttccat gtttcctctc ataaaataaa
     3121 tccttcactc tttcagttta ccacgaaaaa gaaagagaaa aaagagactt tcattgataa
     3181 actacatatt gtagtttata ataaagagct gtagatgaaa attttcagaa agaaggagtc
     3241 taattggctg caagaataaa ggaaaaatta tttaggaaaa gagaaacttg ggaacaaaac
     3301 ttgtttgtct tatggtttgg aacatttatg gctggtatcg gatttagttt agtcatgcct
     3361 tttatgtcac tttacataga tacgttaggt aattatagta cgtcacaatt gaacttttgg
     3421 agcggattga ctttttcatc tactttcttg gtgactacgc ttatctctcc ttggtgggga
     3481 aaattagccg accaaaaagg aagaaaattg atgctgttgc gtgcttcatt aggaatggca
     3541 atcgtcattt ctttgatggg ttgcgtgact agtgtgtatc aattagtcgc attgcgtctg
     3601 cttcaaggga ttttttctgg ttatatcagt aatgccactg cccttgtcgc aactgggacg
     3661 ccaaaagaaa aaagtggtca agttcttgga accttagcaa ctggttctgt tacaggaaca
     3721 ttacttggac cgcttcttgg cgggatctct gcttcagcgt ttggctatcg ccctaccttt
     3781 ttcattacag ggatcatctt atttttcgtc ttcttgttga gtctgttttt cgtccatgaa
     3841 aaatttgtcc cagtgaaaaa agaaaacatg gtttctgcca aacagatttt caaagacttg
     3901 aaatatccac acgtcgtcat cggtatgttc gtcacaacga tgatcatcca agcatccaac
     3961 aattccatta gtccaatcat cagtctgtac attcgccaat tgctgcatgg acatgggaat
     4021 gttacgttga tcagcggaat cattgcttcg atacctggga ttgccacttt gatagccgct
     4081 ccaagatttg gtcgcttagg tgacaagatt gggagtgaaa gaatacttgc gattggtttg
     4141 ggttttgcga ttctggttta tatcccaatg gcattcgtca cgaatgtttg gcaattagct
     4201 gccttacgtt ttttgattgg tatctctgat gcttgtctgc tccctgctgt ccaagcattg
     4261 atcacaaaat attcgcctca tcaagctgct ggacggatct tcagttataa ccaatctttc
     4321 caagcaactg gtaacgtagt agggcctatg atcggctcca gtgtttccag tgcatttggt
     4381 tatcgtggcg tctttctttc gacttctctt cttgttttgg tcaacttctt gcttgtacga
     4441 cacaatacac aagagatcca tcagcaagaa actgcagaag aaaaagcttc aggagtctcc
     4501 atagcttcag ggcactctcc ttctcataga caataagcgt aaatgaaaaa cactgcttga
     4561 acagttgtca cagactgtcc ttgcagtgtt tttgtttagg cctgtttttt caagaactca
     4621 gataagagga ataatccctt ttccaaggtt tcttttttac aacagtaacc tagtcgggca
     4681 tgcttcggta aatcaaaggc acttcctggt actaatagta ctcctgtttc ttctaataaa
     4741 ttgatacaaa atgtctggtc atcttcttcg atggctaatt taatgaaaga agtagagacg
     4801 taattgggag cgacaagttc aactttcggt tcattggcta cccattgttt taaaatcaca
     4861 aggttctctg taatgatctt tttgtttcgc gccaatattt tttctttatt cttcaatgca
     4921 tgtacagcaa gatcatctga caggactccg ccgcaaatca tcgtgtagtc tcgatacttt
     4981 cgaaaaactt ctgccaactc aggtccagta gctgtccagc caatacgaat acctggaatg
     5041 gagtaggttt tggataggga attggttgct ataccttttt catagacatc tacgatcgac
     5101 aagaattcac ctttatccgt taaaggcgca tatacttcat ctatcaatac atatgcatct
     5161 actgtacgag caatttctgc gattttttct aaagttttcc gatctagcaa tgtgcctgta
     5221 ggattgtttg cactattcaa acagatgatt tttgtttcag gtttgactaa ggcttctagc
     5281 tgttctaaat caaactgcca atcttcttct tccttcaaat gaacaaagtc aacagcggca
     5341 cctaaagatt tggggatgtc atataattgc tgataagtag gaagcatcga aacaacatgg
     5401 tctcctgctt ccactaacgc gtatagagct aaaagattag caccagtcgc tccattcgtt
     5461 tgtaagatgt tttccggatc gacggtttga taaagagaag cgacttcttg tttgaaaaca
     5521 tctgatcctt cgatccagcc atagtccaat ggttcgtttt tgcgtttttc gaaaaattta
     5581 cttacgctag tcccatctaa gccaacgagt tcgtctaatg tcaatgcttc gatcgagctt
     5641 tgtgcaatat catataccgc tttcttttcc catttattta gccattcttc tacaccgaaa
     5701 tttgcgattt tcatgtttct ttccccctta tatcttgatc cagcctacag caagcaaccc
     5761 gattgaaagc agtgcggcca cacaaatcag cccaatcatt actttttctt ttcccgtaat
     5821 ttgatgtttg gtttcacgac aagcgatcca gtagaagcaa aaaccaggaa gataggcaat
     5881 actacataaa aggacataag aaaatcctgc taaaatgatg cccatcaatt caaaaactac
     5941 agcaatccca ccgatcacta cttgtttttt gattccttct tttaaagaaa actggacttg
     6001 atagatcccg acaagtagat aacaaatcaa aattgccgct gtacataaag aataggcaaa
     6061 ttgataggca taatcagtaa ctaagaaagt caataagaat agattagtca agattgcagt
     6121 aacaactaac gaataagttg gggcattttt tttattcgtt tttccccaac ctttcggcaa
     6181 cagaccgtct cgagccatca acatcgttgt ttctgctggg agcatcgtcc aagacaacca
     6241 agaaccaatg atcgaaataa tcaagccgat attgatcagc gctgctcccc aatttcccac
     6301 gattcctttt aaaatatttg ccatagcagg ttgtgaaata gttgctaact cttcttgaga
     6361 taagacgccg tatggcaaga tcgaagcaag gatatagata aacaataaac aaagcagacc
     6421 aatgatcgat gcttgctggg cttctgaacg tttcttcgcg cggttggcta aaacacttgc
     6481 gccttcgatc cctacaaaga cccacatcat aaccatcaaa caacctttaa tctgcaacca
     6541 gatcgttcct tgctgccctg ttccattcac tagattatca gatacgttac cccaaaaatc
     6601 agcagtgaat atattcactt taaatgcgat aaatacaaat attaaaaata ggaataatgg
     6661 cactaattta cagatcgtca cgattgtatt gatgacgctc gctccttcaa ctccattgtt
     6721 tactaataaa gtcagacccc aaataaaaat acttgcaaat aaaatagaag gaacattttg
     6781 tccacctttg aatatcggga aaaattctcc catggcgctc atcagcatcg ttgcaaaagc
     6841 aacatttccc agccacgcag acaaccagta accccaacca ctgatgaagc cacctaattt
     6901 accaaatgaa gcttccgcgt aaccgaaaat accaccatct agatctggac gcttttctcc
     6961 taaatgattc agcgataata ccaaagctaa aataccaatc ccaacgatta tccatgaaag
     7021 gatcgctggc cctggagctg cagaactagc cagatcactt gtgatcccaa acacaccgct
     7081 tccgattgac gaactgacaa ccagagcaac cagtccccat ttgctaatac ccgtcgattt
     7141 cttttccatt tccatcaccc tcacttataa aattgaacaa atcacttgca aaagattccc
     7201 ttctattttg caactgctct tgcttaacaa aaagcaactt tattcatatt attggatatt
     7261 atgaactttt tacacaagta tttcaagtat aaaatgaata aaaattaatt ttattgcaaa
     7321 aaaacagcta tgctatccaa aagaaaaggt gtttcttttg ttagccatag ctgttttgat
     7381 tcatttaata tcgcttaaac gtattgtaaa acttttgttt cttgatatgc gtggtcgatc
     7441 aaaccaccac ccagacattc cattccatca taaaatacaa ctgcttgtcc aggagtgatt
     7501 gcacgtacag gttcgtcgaa tacgacttct gcacgattat catccaataa gcgaactgtc
     7561 acaggaacgt cttgctgacg ataacggaat ttcgctgtac acttgaattc ttttggcatt
     7621 ggttcattcg tcgtaaagtg gatttcactt gcatccaagc ttgtggcata caatgcagga
     7681 tgatggaacc cttgccccac atataatgtg tttgtcgcta gatcttttcc tacaacaaac
     7741 cagggttctt gcgaatctcc tccgccaccg atccctagtc cttgacgttg accaatcgta
     7801 taatacatca agccagcatg ttgtcctttg acttcaccat caagagtgac catatttcct
     7861 ttttttgctg gcagatagtt gctcaaaaat tgtttgaagt tcttttcacc aataaagcag
     7921 attcccgttg aatctttttt cttagcagtc gcaaggcctg cgcgttcagc aattgcccgt
     7981 acttctgatt tttccatgcc gcctagaggg aacatcgttt ttgaaagctg ttcttgtgat
     8041 aattggctaa ggaagtaagt ttgatcttta ttattgtcta ctccacgaag catgtggacc
     8101 acgccgtttt catcgcgagt gacttgcgca taatgtccag tagctacata atcggcgcca
     8161 agatccattg cataatccaa gaatgctttg aatttgattt ctttgttgca catcacgtct
     8221 gggttaggtg tacgtcctgc gcggtattcg gctaggaaat attcaaatac acgatcccaa
     8281 tattcttttt caaaattcac agagtaataa ggaatcccga tttgagcagc tactttcgct
     8341 acatccttat aatcttccgt ggccgtgcat acaccgtttt cgtccgtgtc atcccaattt
     8401 ttcatgaata ttccgatcac gtcgtatcct tgttccttta acaaaagtgc tgttacagat
     8461 gagtcgacac cgccgctcat ccctacgaca acgcgtgttt tgctgttgtc tgtcatgata
     8521 tcaccatcat ttcaattaga ttctgaaaca tctacgagtt gaaggtcgca acttttcagt
     8581 attttacatt atacgtcata tttctgaaaa tttcatccct taattatcaa gtcgtaaaaa
     8641 tactgttcta ctggacctat tcagtacaca aacatgatag tcaaccaaaa aatcactcat
     8701 cttttgagca ttcgatataa aaaattggca tactcataaa aagggccagc ctttcggcaa
     8761 taaggaataa ttttataaat tgtgaaaaac tatttttcaa aattgcttct tatcgcttaa
     8821 agctgcacgt ctcatttaca accttttctt tttagatgtt atgagttgct cagataagtg
     8881 attttgttta ctctaagaaa aaagggaggc aaaagataca tgaattactt tatcagtgat
     8941 atgcactttt tccatgaacg cttattagga aaaaacgact ttgctccgcg gctctttgca
     9001 aatgtggatg aaatgaacca acagttgatc actagctgga atgcagtaat aaaagaaacc
     9061 gaccatgttt atcatctagg cgaccttgct atgcatccgc aatatgaaaa agcaaatgca
     9121 gaaattttgg aactggtcac tcaactgcaa ggaacaatcc acttgatcaa agggaaccat
     9181 gacagtcgcg cttttttccg ttatttagaa aaaaatgatc ctaggcttac tgacggaagt
     9241 cccaaatttt atttttatga tgtaggtgcc atcgttaaat tcgaccatca acaatattac
     9301 ttgactcatt atccgatgtt gctcgggcct aacgacacta tccgcaatct tcatggacat
     9361 atccatcatt acagcgtacc tatcgagaac gatgtcaatg taggcgtaga cgcaccggaa
     9421 agagaactct taaaggaaaa actaccattt ggcactccgt tatctgagca acaaatcaaa
     9481 gaaatctatg aagcaaagag gaagtacgat aggtcgtgaa aaaactgccc tatatcaagt
     9541 attaaacaaa taaagataat tactcatatt tttcatttga gtaggcttat tgccacagga
     9601 acattaggat cagaaatgga gttgtaacaa gacttctgtc acaactccat tccatactat
     9661 tcgtcttttt gctcatggtg ttcttttacc agttttttct gtaattcttt atcataattt
     9721 cgggcagctg acgttaaaaa taaaacgatt ccgcataata aaatcccgat tccggaaaac
     9781 aagaacattt tttctacccc taaacgatca gctaatggtc ctgcaaataa taatccaatt
     9841 ggacctgcca ggttcatcaa agagttgaag ttccctaaga cacgacctaa aacattcgat
     9901 tcataactct gctggatcat tgccatcagc agtgtattga aatatggcgt tgcacaacct
     9961 gctcctgcat tcaagataag aaagtacaag aacccttgac tatcgtttgg aaccaatccg
    10021 cttgcaccga tcgtcaggcc cataatgatg taggctataa taataggttt cattcggtct
    10081 ttccatgttc ctataaaact gatcagtgca ccgcctaaga gcattccgac tgcgtagact
    10141 acttccactg ctcccgcctg tccaacggaa ccaccaaagt aatctagcgt catcaatgga
    10201 tacagactga ttgctggcat gaacaacagc atcacaaatg caccattgat cgttatgtac
    10261 cataaccctt tattatccat caattgctgc agcccaaatt ttgcatcttt aaaaatagta
    10321 agcggaactt ccaatatttc aggagaaact tttgggatct tcacaaaaat caataagccg
    10381 acaccaaaaa ctgcccccaa tacatctaat aaaataaggt aattgacagg aacgactgaa
    10441 aacaaagccg cacctagagc tggcgcaatg atgaaattcg ctgattggac cattcctaac
    10501 tgtccattcg tccttgttat gtgagaagat ggaacgattg tcggcataat cgactggatc
    10561 gttggcattt ggaatgtttg tgcgaccgaa cgcataaaca aagacacaaa aactagccaa
    10621 atcgggaagg attctgaaat cgttccgact actgctaaaa taagcgcgaa aatcgctaca
    10681 ataatatccg tgacaatcaa aagagctttt ttatcccagc gatccactag cggtcctaca
    10741 aatgggctta gtaaaaccat gggaatcatt cctagtaaag tagcaaaact caaaattgtc
    10801 gccgaaccgg tttctcttgt caaataccag atgatcgcat attggacgac catactagtg
    10861 attcctgaca aaaactgtcc cgataaaaag aacaaataat ttctcttcca attcgttaaa
    10921 acgaccgact gttcattttc cataaacatc cctcttttct ttttcatttt gtatagcgtt
    10981 tgcacaacaa tcattatacg ggattctttt ttcaaaaaat acctttttcc ctaaataaca
    11041 atcgtttcaa tttaaatttc tttacttaga aacaataaat cagcaaaata ttaaaaataa
    11101 caacatgtgt taaatttaaa aataaaaaag acaactaatt tgcgtcgtcc ttacaaagtt
    11161 agtcatctaa caataggccg tttcaaaact agttgtcttt ttatatccac agatcattcg
    11221 actttttcga ataacccacg gctaatcatc ccatccatca gaaaataaaa tttatcttgg
    11281 ctcattttct gttttggatc tttgaaaata ttgactgctt tcaatccatt tgctgtagtg
    11341 atcgacattt tcaatgtttg aatatctttt gatacagtga tttttaattt gaatccttct
    11401 ttgctttgcg gcgtggcatc taatggtcgg atcaattgat aattgcctcc gtttgtttct
    11461 gtaaatccat tatcgcgcaa tgtatatctt tttgcatcag gatgcaaacg atagctaatt
    11521 ggtgcgccca gtaccgttgc tgttgtttca aatgccatga ttttcacctc gataaaatta
    11581 gtttctcttt attataaaag tttatcctga tttttactag gtttttcagt taagttccct
    11641 aaaaaatgaa ggcgttctct cagacatctg ttcatataag aatgacttca ctagttattt
    11701 tttgacagca cgaactaatt gatcaaggaa atactggatg tcgtcttcgg tatttcccaa
    11761 accaaagctg atacggatgg attcttttag tatcggtgta tcttctccat acattgcttc
    11821 taatacgtgt gaaggctcga tatttcctgc tgtacaagct gaaccagtag aaatagcgac
    11881 cccattaaga tcaagcttca tcagtagcaa atcatttgct tcgccaatta agcgaagatt
    11941 caacacatga gggagtttat tcgttaaatc accatttact tgataaggga tttcgtttgc
    12001 ttctaatgta tcaagaatca attgctggaa tcctttatat ttgttgttac gtgcttcacg
    12061 ttcttcaggt gacagcagtt caactgcttt tgccataccc cagatccctg ctaaattttc
    12121 tgtacctgca cgccgctttt cttcctgctc cccgcctaaa agcaatgcag ggatatttac
    12181 tccgtctcgt ttatataaga aaccgacgcc ttttggccca ttgattttat gagcagagat
    12241 acttaataaa tcgattccta attcttctgg atggatcgtt tggtttccgt acgcttgtac
    12301 cgcatctgta tggaaataag ctggatgatc cgctaaaagt tcaccgattt ctttgattgg
    12361 catcaaattt ccgatttcat tgttcccgta catcacagag accaagatcg tatcttctcg
    12421 taacgctttt ttgacctctt cgatcgacag attccctttt tcatctacag gcagatatgt
    12481 aacgtcaaac ccctttgtct ctaaatattt catggtatta agaacagcag gatgttcgat
    12541 agctgtagtg attagatgtc tgccttcttt ttgacgcgag aacgctgttt ctaagatagc
    12601 tgtattatcc ccttctgttc ccccactgtt aaagatgatc tcgtgaggtt ttgcttgcag
    12661 actgtctgca atgatttgtc ttgctgtttc caatttttca tgcgcgtggc gaccaaatcc
    12721 atgtatgctt gaaggatttc caaacgtgtc ttgcatgatt gccgtcatcg cttcaattac
    12781 ttgtggatgc atgggggtag ttgccgcatg atccagataa atagttttca cttcattcat
    12841 cccttccaaa ttattgctag gcacattcta gcataaaata gcgaacaaac caaagaagag
    12901 ctcatttttg tctagaggac gtttcatggg catagaaaaa aggagctgtg acaaaagtct
    12961 tgtcgcagct ccttgaagct gctcacttct gtcacaactt ctttccctct tttttttgtt
    13021 tattcgaatt ctttttcttc cagacggaat aacggactca tcggtgtatt ttcatggatg
    13081 cgtttgaccg cttcacccat taattctgaa catgtaacga ttgttaagtt ttctggatga
    13141 cgattttctt ctgtgaaaac agaatcagtg atacaaatat ctttgattgg tgcattgtct
    13201 aaaatacttt tcgcatgatc ggacaataat ccatgtgacg cacaggcata tacttctttt
    13261 gcgccgtttt cttttaatac atttgctgct cgtgcaagcg ttgctcctgt gttcaagata
    13321 tcatctacca tgatacatgt ctttccttga acatcaccga tcacataacc accctcagtt
    13381 gaagcatcat ctgcatgatc tacgatggct aatgtgctgt tcaaatattc tgaaagacta
    13441 cgtgcacgct gaacaccgct gttttttggc gaaacaacta cgatgtcatc tcccgtcaga
    13501 cctaattgac ggtaataatg agcaaacaac ggcatcgtaa ataaattatc tacaggaata
    13561 tcaaagaatc cttgtacttg aaccgtgtgc agatctaaag tcagtactcg agttgcccct
    13621 gcttcttcga tcaagttagc gatcaatttc gcagtgatcg gttcatgagg tttcgccgtg
    13681 cggtcttgcc tagcataccc atagtaaggc aatacgacat tgatcgtttt cgcacttgct
    13741 cgcttcattg catcgatcat gatcagtagt tccatatagt aatcattgac aggtgcattc
    13801 gttgattgaa cgatatacac atgatcccct cgaatacttt cttcgatatt gatggaaatc
    13861 tcgccatcac taaattgctt cacgacgctt ttacctaatt ctgttccgaa cactttcgcg
    13921 attttttcag ctaatggacg gttgccattc aaactaaaaa tcttcaacgt gtcatcctga
    13981 tacttattac tcatgtagtc ctccccaaaa cttctctatt cttcgatagt ttaacatatt
    14041 tttccaagat tttctgttaa tttcagcaaa ttcttaagga agtgaaactg ttccgttact
    14101 ccaactggca ggaatgactt tatgataatc gccccagttg tcgtgtactt tgtaataacg
    14161 ttctcctgtt tcagtttcaa aataagcgta cgctgtcacc caatgattgc catatgtact
    14221 gccaagtact ttcaaaatac caatcatcac aggtttgcct tcacgtatac gttttgtagc
    14281 tcgctgccat gaacctagaa gagttgctcg tgcacgctca tgattgccat tcttctttaa
    14341 aaaactagaa atccctgtac taacctgaaa aggaatcgta ggaagtccta gtggttggat
    14401 ctggctacgc aatgccttcg tcaaaaccaa cgaatcagcc gtatttttct tgcgaatctc
    14461 atttggaagc atccacccct tacgatagtc ttgataatag gcaaggagaa ctgctgccgc
    14521 gtaagtacca caaagatagc ctgagggggt agcccatttc ttatacctca aaaaacgttc
    14581 ttctggcaat cccacccagc aatttgcgag ttcctttcct gacctttttt cttcgatcaa
    14641 aacgggaata tcttcttttc tgttgatcca tctagataac tcgattggct cttcattgat
    14701 cagcttccat tttttacaag aaagaccctc ttgataagta tattttgctg gagaatattc
    14761 taccggatgt cctgattgat cgataatcac ataactttga tcttcaaaaa ccagagctaa
    14821 aaaaagcaat ttactttttc ttgactgcag ctgctgagtg ctggctaatt ttttaatttt
    14881 tttatctact ttgaaatata aaggtttatc cactacttga ttcagttggg atcgcctcct
    14941 ttttttcatt ataagtgaag gattgaaaaa aacaaatcag tttgattgtt cgaattttca
    15001 attttttatg ttctatttgt taattccttc attttgtaag ataatgggca cacgaaacaa
    15061 aaaaggagcg atcgatgaat gaacaaagaa cgattgaaac aagatttcta tgaagcagtc
    15121 aatgaagaat ggcaaaaaac agcaaaaatc cctgcagata aaccagctac aggcggtttc
    15181 caagatttag ttgacgggat cgaccaactt ttgatgggag aaacagacga tctgcttgcc
    15241 catccagaaa aagtgtcttc agaaaaaatg aaacatttcc ttgcatatta tcaactggca
    15301 aacgattatg accgaagaaa tgagttgcat gctcaaccgc ttcaacctat aatcgaaaaa
    15361 gttgaatcat tgcagtcttt cgacgaattg aataagcaat ttccagaatg gctgttggat
    15421 acattaccat tgccatttaa tctggatgta gatgcagata tgaagaatgc gcaaacgaac
    15481 gccttctttg cttatccgcc ttctttattc cttccagata agacgtatta tgcatcagac
    15541 catcccaacg gtgcacaact aataggcgtt ttctttgata tggctgttca gttattagag
    15601 cttgccggca aagaaaaaag tcaggcagaa gcaatcacgg aacaagcagt gcaatttgat
    15661 aaattgattg ctcctcatgt aaaaagtgca gaagaaaacg cagattacag caaaatgtat
    15721 aatccgcaat cttttgatac atttattaaa tacacagata aactggactt gaaaaaactt
    15781 gtcacgggtt tgattgggac aactccagat aaagtgatcg taactgatcc agtgtacttc
    15841 gatcatttga atgacatcct gacagaagct cattttccgt tattcaaaag ctggatgatc
    15901 attaaaacag tacgttctct tagtggttat ctatcagaag aattccgcca agtcagcgga
    15961 atctattctc gcgtattgtc tggaacagac gaagcaatgc ctcagaaaaa agcagcgtat
    16021 taccttgcct ctggacaatt cgatcaagta gttggcgatt actacggaaa aaaatatttt
    16081 ggcgaagctg ctaaaaaaga cgtagaacaa atggtgaaaa aaatgatcgc cgtttataaa
    16141 aaacgtctag aagaaaatga ctggcttggg gatgcaacaa aagaaaaagc cgtcataaaa
    16201 ttaaataact taggtatcca agtaggctac cccgataaga tcccagcatt attcgaccag
    16261 ttcaaaacag tacctgcaga tcagggtggt actttgcttt caaatactct tgttttcagc
    16321 aaactcacat taaaagatcg tttcagcaaa tggaacaaac cagtggatcg ttcagaatgg
    16381 gaaatgagtg ctgacacggt caatgcttat tatcatccat ttcggaatgt gatcgtcttt
    16441 ccggcagcga tccttcaagc accattttac agtctagaac aatcaagctc tgctaattat
    16501 ggtggaatcg gagctgttat cgctcatgag atttcccatg catttgataa taacggcgct
    16561 ttatttgacg aatatggcaa cttgaacaac tggtggacag aagaagatct tacacaattt
    16621 caaaacaaag cacaagcaat gatcgaagaa ttcgatggtc tgccttttgc tgacggaaaa
    16681 gtaaacggaa aattaaccgt ttcggaaaat attgctgatg caggcggtct aagctgtgca
    16741 ttggaagcag caaaaacgga atcagatctt tctatcgagg aattctttgt caattgggca
    16801 acgatttggc gtacaaaagc aaaaaaagaa taccaacaac ttttactgca aatcgatgtc
    16861 catgcccctg ctaaactccg agcaaacgtg caaccaaaaa accttgacga attctatgaa
    16921 acatttggta tcacggaaca agacgctatg tatctagcgc cagaaaaaag agtgcgaatc
    16981 tggtaagagg ttgtgccaga agtggtcagc ttcaagaaat aagaaggaat ttgggaaaat
    17041 cgcttctcaa atttttgtga catttcggct tatttttcga agaagttact tctggaatac
    17101 catgtattag aggttgtgaa aaagctgctt agtttcaagt agtaagaaaa attttgtgaa
    17161 aatggctttc cacatttttg cataaatttg acttactacc gagaaactgg cttttgaaca
    17221 ccgtttatcg agaggttgtg aaatcagcgt cctgacctga gtattagaga aaaaaacgga
    17281 aaaatagttc ctcatatttt gccgtttgtt gagctaatac cgagggtcag ctgattgaac
    17341 accgtttagt ggagagattg tgaaaaagct gtcctgcatc aagtaataag aacagccaat
    17401 caaaagcagc aaggagaacc tcatctataa aaagatagaa ggaagtgaga catctattgg
    17461 tctcatttcc ttctattttt actttcctca gtcaattgtt ttacatcata aaaggagaaa
    17521 ttccacttat ttttttcgct gtttcatgac ataaacaact acaccgatta acaaaattcc
    17581 tccagctaat gctagcaacg ggttattaaa ttctcccgtt tttgggagtc cttgagtgcc
    17641 atttttttca ctttcttttg ctgtcctatc cggatgagaa ggttttccat ttggttttcc
    17701 atttggtgat tcatttcctg gattttctct ttctggtttg tcatttcccg ggggcacatc
    17761 ggcagtttct cgcacagtga tcgctttttg ctgggtaaca gattctgtat gagcagacca
    17821 tttttcccca tcaaatattt ccgaacgata agtgacttct agcgtatatt ctccttttga
    17881 aggtgctgaa aaaatagcgg tatgattaga aaattgagca cttgactgtg tttcaacgat
    17941 ttggatcttt tctgggaaat aacgcatatc ttcaaagaca ggcgttcctt gatttggtgt
    18001 gaaagttgac acaaaaactg taaaaggctt cagttcaatg atatttcctt ctgcattctc
    18061 cgccacacca tttgcttctt gtataataat cggattattt tgttcatttg gttttgtctg
    18121 atttggttct tgtacgattg catactctgg atattccgaa gacggtgagg tttctccgtc
    18181 aaaaatagca cctacagata atggaagacc atccactctt tccttttctg tttgacgatt
    18241 ccccggttta atctgcatca gtccatgaac atggatactt ccattttcat agcgcgtaac
    18301 aggctgctga ccttctttcg aacatgtatt tagagactcg atccaatctt ttgatatggt
    18361 atgtccaaga gtattttctg attgtttctc tcgttcattc caatagtagt tcaacgagtg
    18421 gtagatttga tcttcgtttt gattcaatgg gcggatctgc tctctcatct caagattctc
    18481 tgttcggaaa gataagatac cagaagcatg gtagtctgtg tttcctgctg aagaggtata
    18541 aagagccaca ttatatgaac cgttattaaa acttgtactg ctattgacga taatatctgt
    18601 tccgctattg gaatcgattc ctttcgcaag attttcgaaa gacaaactat tgatcaattc
    18661 atgagggccc gttaaagaag aaccgcccat tttgaatcca ttaccgttac cttctccctc
    18721 aattcctggt acccagccgt ttcgataagc aactgagttt ttgatgatta cttttccgat
    18781 cgaacctgtt tcatttttag caaaaaggtc ccaaccgtca tctgcattat gatacgcgat
    18841 acaaccgtca aatacatttc cttctcctat tgtcagtttg gccgcaaatc catcggcatc
    18901 ttcaaatcct ggatcggcat tttcaaaaga tgtacagttc ttgactaagt tatgcgcggg
    18961 ccatgcttcg aaaggatcat ttgagctacc gctgatttgg attcctgtat ttccattccg
    19021 ataagcatga atctcttcga tcaaattatg atttccagaa atctgtaatc ctttttgcat
    19081 agctgcacta tttgtaatgt caaaccctcg taaaccccag tagtgactga acagtgtcat
    19141 cccatttcca gttcctttaa aatcaagtac aggacgcttg ttttctggat ttttctctgc
    19201 aatcaactga atcgggtttt ctcttgtccc gttaacgcct tttggaatcg tcaatcctct
    19261 ttcgaaggaa taagttccag gcgccatgac gattgtctgc ccagcatatg cgtatctcaa
    19321 tgcttgtgtc agagataatg gattttgtct gcttccgttt cctgcatctg acccttctgg
    19381 tgttacataa actgtcgaat atttaggttt acggtagtcc actgtatgaa gaatctcttt
    19441 tgtttcataa ctagtcatct ctacgtattc ttctggcgga tagtttttat ctggcgtaaa
    19501 actgaaagta aattcgttcg ttccttccat cagttttgtc tgataatcaa attcccgatt
    19561 cgcagtgata gccaagtttt ttgcgttctt aagcacctct cctgttgctt tatttttaat
    19621 tgtcaataat ccgtcagcat ttgctctaaa ggacatagta tgatcttggt ttcctgaatg
    19681 attactagag gtcacagtgt aaatcggatc aatcaagcga gtaggtcttt cttgcccagg
    19741 aggatctgtc gcaggatctg agtaagcaac ttcgattcct tctaccttga tcgtcatatt
    19801 ccttgctgca gcaaatcctg cataaataac cgattcatct gaagcgtata atttttccca
    19861 gtcatacatg atgtcagaag ccatttcttc tcctgtttca ggatcgtaat aatatgcctc
    19921 taaaccagta ttcgtttttt tcatctctac gtccagttta gtaatcgagt tcgtggccgg
    19981 aaacagattc ccatgaccgt ttccgaatat attataagaa cctgtttctt tttccaagaa
    20041 ccctgctgaa gtctctaaag gtgttgtttc cactgagaca ctcccaggag ctggcgcagc
    20101 ttgcggatct tcagatgata ttcctgtgat tgcccgtgtc cccaatccaa gtcgcatcgt
    20161 atatttgctg ccgctcgtgt tgaccacttc ttgtctatcc gagtcccaga catattcgat
    20221 ccgactgcct agtatggcaa atgaatttga ataaaatgcc tgcccaaaca aatgattttt
    20281 tggtactgcg tctcttacca ttaaagcaaa tccttcttgg gcattcgtat aggtccaaga
    20341 ttctatttcg atcgtcgccc gcatcctaaa gttgatgtct ttggggattg cttgataata
    20401 ataggtcatt ccatctgatc cgcttgactg gaattttccg ccgttattcg tggaagagag
    20461 ccgcacgccg tcttctaatg gttcaatggc atacctacta gcacttgtgc tttctccgaa
    20521 aacaacccct ttccactctt catttgcttc ttcagattgc ttttttgtct tttcattttc
    20581 tgattctttc atcataggat catttccttc ttccgtatcc atatcttcac tagttcttgg
    20641 tatttcctcc attgttgaca tcccttcaat gttcacgtgt gcattttctt cgttcacaga
    20701 ctgttgttcg ttagcagcaa cttcaacgac agaaattaaa accggggtac tgagcataag
    20761 gagattcatc attagcgcac tgtattttat acgttttttt aacaagtgta ttcctcccgt
    20821 aacaaattag aaagcgttta cttaaagtat tacctaattt atttttactg tcaataaaaa
    20881 acaaaattat aataagtgta ttttataaaa agaaaaaata taacttaagt tgttgataat
    20941 aaagagattt attaacgtaa aaagaataag tttttttaaa ataacgtata aaaaagagca
    21001 taaaacaaag tcgtttgttt tatgctcttc ttttattatt ttgtgctttt tattttatca
    21061 aatcactttg tttttttata aaatttctgc gcctagtgtg gactcaaaat gtctcaatgc
    21121 ccattcatgt ccaacgggat cgaaacttgc cacggcatct ttgaaaatat ggagtttgta
    21181 tcctagatta tacgcatcga ctgctgtatg caacacacag atatctgtac atacgccggt
    21241 caaataaata tctgtgatct ggcgttctcg caaacggatg tctagatctg ttccactaaa
    21301 agcggaataa tgccgtttgt ctatccaata aacattttcc tcttgctcat agtcttggta
    21361 taaacttttt aaagagccgt acaattcccg tccacccgtt cctatcacat tatgcggagg
    21421 aaataagcgg ttctccggat gaaaggaatc ttttggatca tgcgcatcaa tggcaaaaac
    21481 gacaaaatcc ttttgatcaa taaacttctt tgtatgagca gtcagctttt tttcaattac
    21541 ttgtcctgct gtcccagtag tcagctttcc atcctctgca acaaaatcat atgtgtagtc
    21601 aatcgagatc aatgcgttca tcttagtcct ccttatacga gatgtttgac aaattcgatc
    21661 accatttctg ctgatcgttt gcctgcgtct tcgataaatt cgtcaaagct ttgtgtcgcc
    21721 gagtgatctg ctgtgtcgct cattgcacgt acgatcaaaa atgggatatt gaactgacga
    21781 gctgtttgtc caacagcggc tccttccatt tcgcaagcta atgcttcagg gaaattcgtt
    21841 aagatttctt tgattttatc tggcgagtcg acaaatgtat cacctgtaac gatcaatcct
    21901 tcttttgcgt tcaaattcgt tgcttttgct gctttaacca tttcactgcg caaatatgtg
    21961 cttgcttcat aatatagagg cattcctggt aattgtcctg ggttgtagcc aaatcctgtt
    22021 gcatctgcat caaaataagc tactttgtct gatataacga tgtcacctac ttgtaagcct
    22081 tcaccgattc cgccagctga acctgtattg atcaccatgt tcacgccgta ttgttggatc
    22141 aacagacttg tggtaataga tgctaagact tttcctatac cggaacgtac gacgatcact
    22201 tcgtggttac ctaatgaacc tgaaataaat aaagcgcctg cgcgttccca tgataatggt
    22261 tcgcttaggt tctctctcaa aatttttact tcttcttcca ttgcgccgat aataccgatt
    22321 ttcatctttt taattccttc tttctatata aatgcgattg ctaaaaaaac cacacataac
    22381 attaggataa caataataag agatttcatc aaaaaagagt tccatttggt cgtcttttcc
    22441 cgttcgctga tccttgtttt cgtgattggt ttattctttt tgctttcttt acgataaaag
    22501 ctggcaatct ttttttcttc ttgctgataa gctgtttctg cttttcgctg tttttttaat
    22561 gactcgcttg cttgtgcttg ttgtcgcttg cgaagttcgc ttcgcgtaat aagcggtcct
    22621 ttggccataa gccgatccct ctactttcct ttggtcaata gttcccagta ttgaatcgca
    22681 taaatcgtct ttgcatcaca aatcaactga tctttcatcg cttgttgcgc ctcttcaaga
    22741 gttagatgat acaactccag tacttcgtct tcatcttgtg ctaaaggatt ctcgaccttc
    22801 tctacccctt gtgcatgata aatatgaagg acttcatttg caaaacctgg tgaaagatac
    22861 atcgaagtaa gatggctcag cgacttcgca cgatagccag tttcttcttc taattctcta
    22921 gctgctgtca tttcgggatt ttgtccttct cctggatcga tttttccagc tggaatctcc
    22981 aaaatcactt tttctaaagg ttttcgaaat tgtttgacaa gaagcagacg atcttgttcg
    23041 tcaaaagcaa tgatcccgac accgccagga tgaaatacca gttctctctt cgaagttcct
    23101 ccatctggca aacgaacttc gtctacagca acatcaataa tttttccgct atagatttct
    23161 ttgcgactga ttgttttttc ttcaaattgc tcattgttca gcatatcttc tcctgctttc
    23221 tttgtaatca taactgcttt tttttcattt ttcaattttt ctcaaaaact ccttattagt
    23281 ttaccataga taaaccttag attttctcta aatatagaaa atccttcttt agtccgatac
    23341 tctttctagt agtccgtatg ctaaaataag tatgtaatcg aataacgaaa agaattcaat
    23401 actcaacgat aaatgacatg gagggtttat tatgataaaa cgaaattggc cgtggcttct
    23461 agcattggtc ttgctatttc tctttgtccg aaacaatagt ttttcttttg ttctaggact
    23521 tttattgata ggtgtagttt tagttttgtg gggactcttt ggttcgcgca ggaaaaaggg
    23581 aaaacaagaa gaaattcctg cactgactga cgaacttgaa actcactatg aaaaaagcgg
    23641 tatgacagcg aatgaaattt cattttttcg acaaacaatg aatcaaacaa aaaatgagat
    23701 caatcaattg caaatgaata tgcagcagac agccaaactt aaagcaatcg atctccgcca
    23761 tgatccagtc aaagcagcga aagctttatt caaagaattg gtgaaagaac caaaccgact
    23821 gcatgaagca agtcagtttc tttatacgca tctgccgaat ctagttgatt tgacaaataa
    23881 gtataatgag atcaatgacc atgagatcaa aaacaaacag acctatgaaa aactagaaga
    23941 aagcgcgcag atcatcgacc agctgtcagc tttaatcgca caagattatc aaaaattcgt
    24001 tgctgaggat ctagatgata tcgacgtaga gatatctgtt gcgaaacaga gcttaaagcg
    24061 tgataataaa tatgaaccag aacaaaagga ttaactcttt tgttcataaa agaagactcg
    24121 gaacattcag atgatgtctg gctgtttcga gtactttttc taaagtagag acattcatgt
    24181 aagaatcgag aacagattca acaaggagga attttttatg gatcataaac cgactgaaac
    24241 aaaagatgtt acaccagtta acgatacact tgaagattta ttgaacaacc cgttttcaac
    24301 tccaacagat acgctgacca cttcacaaca agcggagatt gatcagctcc aagagcaaca
    24361 gacagctgct cgtttaattg acaaactacc ggcggaacgc caagaacaag ctaaacagct
    24421 agcagcaaaa atcgatgcta atgatgcaca gtctgtcatc agctacggat cggctgccca
    24481 agcaaaatta agtgaattct cacaatcgat gttgaatcat gtgcaagcac aagatattgg
    24541 tcctgtagga gattccttaa ctgaactgat gtatcgtctc caagaagcca atcctgacga
    24601 actgcgtgcc ggagaaggga acatcttcca gcgtgtcttc gggaaggtca aacaatcgat
    24661 ctatgaagtg acagcaaaat atcaaaagat tggtgcacaa attgacaaga tttcagtgaa
    24721 attagataaa gaaaaagacg gtctgctaaa agataatctg atgttagaac agctttacca
    24781 gaaaaacaaa gactactttg atgcactgaa tatttatatc gctgctggag aattgaaaat
    24841 ggaagaactt caaacaacga tcattccaga agcgatgaaa agagcagaag aaactggcga
    24901 tcagatggat gttcagattg ccaatgatta cacgcaattc ctagatcgtc tggataaacg
    24961 tacgcatgat cttcgcttag ctagacagat cacgatccag caagcaccac aaatccgttt
    25021 gatccaaaac acaaatcagg ccttggctga aaagatccaa gcatctatcg ctacagccat
    25081 tccgctgtgg aaaaaccaag tcgtgatcgc attgaccttg cttcgtcaaa aagatgcagt
    25141 aactgctcaa cgccaagtct ctgaaacaac aaacgactta ttgaagaaaa attcagaaat
    25201 gctgaaaatc tcagccatcg aaacagcgaa agaaaatgaa cgtggaatcg tggatatcga
    25261 gacccttcaa accactcaaa atgatttgat cgaaacaatc caagagacat tgcgtatcca
    25321 aaaagaagga aaagaaaaac gtcgtcatgc agaagtagag cttggacaca tggaagaaga
    25381 tctgaaacaa aaactgttgg atttaaccca atagatcacc tttcgcttgt tactaaaaca
    25441 aacgcccaac aacagtttat tacaactgtt gatgggcgtt tttcttatct ttcttctaac
    25501 ttctccattg gagtctattt ctcattcatc ttgctgtgtc catagccata gccaaaataa
    25561 atgatcagtc caataaccat tacgataccg aacacgatcc atgtcacagt ttgcagacga
    25621 agcatcaata caatacaagt caagatggag acgattggca atagcggaac gaatggcact
    25681 ttgaattctc cagcttttgg tttgccaaac atttttctca acttcagtaa gcctagacta
    25741 acaagcatca aataggcgag cgccatgata ttcgttagtt ccgctaatac attcaatggg
    25801 aaaaatccgg caaagaatac agctgccaca ccagcggcat aagtcgcttt tttcggtgta
    25861 cgatgctttt gatcgatttc tgtcataaac ttaggaagta agccatcctt gctgacagca
    25921 aacagtaaac gagcaagtga atacatcatc gagatcgtta ccgtcagtag cgttaaaatt
    25981 gcccctacag cgatcaccgc gcccactgtt ccatgtccta catacctcat tgcaaaagcc
    26041 actgggtcat tgactcctag ttcagtatac ggaacgattc ctgtcaagat cagcgtcacg
    26101 atgatgtaaa ggacagtagc aatcagtatc gaaccaatga ttcctcttgg cacatctttt
    26161 tgcgggtttt tgacttcctc ggctgccatt gcaacggcat caaatcccaa gaaagcaaaa
    26221 aacacaagag ctgcaccatc tagcacaccg gaaaaaccaa aaggcatgaa tggctgccag
    26281 ttatcaggtt taacgaaaaa tattcctaca atgataaaca acgcgatgat accaaatttg
    26341 acatatacca tggcattatt caaacgtaat gctttcttgg cttcaagcga cacgatgtaa
    26401 gtgacgaata tcactacgag caccgcaatc aagtcaatgt atgtaccatt ttccggattg
    26461 tacccgccag ttaatgcttg tggaagttcg gcccctaaac ttttcaaaaa accttgcata
    26521 tagccagacc atccggaagc aacagaggaa accgctagta aaaattcccc gatcaacaac
    26581 caccctgtca gccaggcagc aaattcacca aatacaacat acagataggc atatggccca
    26641 ccaatcactg gcactcgcga agcaaattct gcgaaactta gtccagacaa aatgacaacg
    26701 attgcagcta agacaaatga aagtgacagt gctggaccag cattttgatt ggccgcaacc
    26761 cctgtcacca caaatattcc cgtaccgatg atcgcaccaa ttccaagcat gatcaaatca
    26821 gacgttttta gttctttttt catctcactc gtatgattga gattgatctc tttcttacga
    26881 aacaaatcca ttgaagttcc tccccatttt aggttggaaa acagcgatta aactgttttc
    26941 atatacccta aaaggatacc acctaaaatg actaaaaggc aaccaacaat aacataaatg
    27001 agctcttttt tcgtttttct ttcgccaagg atgaaaatcc cacctaaagt agagataatg
    27061 atccccattt gtgaaagtga gaagccaaca gctaaaccaa gtgatttgac tgttagaagc
    27121 atgcagatgt ttcctaagcc ccataataat ccacagatca tgtttttcca cacaaactga
    27181 tcgactttaa ctttacggaa agcaaagaag cttgcgccta agatcattcc gatactttga
    27241 ggtaagataa ttgacatcgc atccaaattc gcccagttga cgatgatggt atatacacca
    27301 taaccaatcg tagagaagat caatgctcta aaacccttac caaaatcaag cattttatta
    27361 tctgtatcta tttttccttc gtcatcttgt cgggcagtca aatacgcacc gcttacaaga
    27421 agagctaaag caataatacc gtatacataa tctttcgttt gtgtccattc atggaagaaa
    27481 acggctcccg caatcgtatt caaaattaat tgaaaaccag tagacaatgg tagccctaca
    27541 gaaacaccca tgtacttcat tccatggaac tgtccatttt gtccaacact ccaagctaag
    27601 ccggataaaa atccgatcaa gaaaatccaa ggggtgatta caggactaac aacgaaaaag
    27661 acaactaaag aaaacagaaa tgcaccgatc gtcattccta gtgtttgttg attggcatcg
    27721 ccaccaattt taccacttac taaaccaata ctaccccatg ccaacatagg gactaatgct
    27781 attaaaattt ccataaccaa tctcctttta tttcacatga taagaaactt atcagcgaaa
    27841 acgttaacaa cacaagcact ttttttcgtt tctaacgatt ttctaacgtt tttcattata
    27901 aaaatttaag attgggaaaa ataaagtcat tgttgtccct tttccttcag ttgattcagc
    27961 cgtcaaatgg atacctaatt tttcggccag attttttgct aaataaagtc ctaatcctgt
    28021 tgaatgtgtt ttgctaagtc gaccatttct cccagtaaat cccttatcaa agatccgttt
    28081 caagtcttcc gctgggatac cgatccctga atctttaact gaaaggctga ctccttgcgc
    28141 ttccttttct atgctcacaa tgatgtttcc gtgatccggc gtatatttga tggcattact
    28201 aagcaattgg ttgaagataa aagcgatcca tttagcatcg gtcaaaacag tatgatcctc
    28261 accaacgatc tcgtagcgaa tatttttttg gataaaataa tttgcctgac tgcgtatcac
    28321 aggctgaatg attcctttta gtccatattc ttggatgagg tagtcccgcg agaaactgtc
    28381 gagtcttgca taatacaaaa cttgttctac gtattcatcg atttttgata attcattttc
    28441 tagtaatgta tacttttgat cgtcaatatc gaactcgatc gaacgaacta gcaatttaga
    28501 agcggaaagt ggcactttga tttcatggac ccatgagtca atataatctt tttggtcttg
    28561 ttgattactg attgctgatt gcattacttc ttggtgttca cggatcagct gattgatata
    28621 ttcttctacc agtttttctt cttctgtcct cccaccactc aaataatttt gcaaagggga
    28681 agttttttgt atgtcggcta attttcccca ccatcttctc ttgctcaagt aaccacccag
    28741 cagataaaaa atcagaaaga cgccctcaat caaagctaaa taagcaattg tgctcaaatc
    28801 aactttgatg tttggcgcca gccacatcac aaagatcgtc aatgcaataa aaaacagcca
    28861 tcccaagatc aatgtccact gatctttcaa atatttaaaa aaattcatct taaccgctca
    28921 tcctttcagg gaacgatata tccctgtcct acttttgttt cgatatatcc ttggatacct
    28981 gcctgttcga tttttcggcg taaccgattg atgttgactg tcaatgtatt gtcatctaca
    29041 aatcgttcgt catcccacag tgctcgcagc aatttttccc gacttaagat ctttccattg
    29101 tgcttcatca aaataaataa tagacgatat tcatttttgc tcaaatcaat gatctctccg
    29161 ttgatttcca tacttccgtt gtcaacattc aacgtaatgc cattgtggct catcatttca
    29221 ctagataatt ctgtgtagtt gtaagaacgg cgaagtaatg cattgatttt tgcaatcagt
    29281 acatcgattt ggaaatgttt tgtcacaaaa tcatctgccc ccatgttcat agccatgatc
    29341 atatccatat tcgtattcct gctggagata aaaatgatcg gtactttcga tacttcacgt
    29401 attttttgac accaataata gccatccaaa acgggaagat taatatctag taatacaagc
    29461 tgtggttctt ctctttcaaa gtctgagaac acttgattga aatcggtcgt tccaaaggtt
    29521 tcaaattgcc atttctcaag ttcctccatg atcaattgtc gaatgatctc ttcgtcttcg
    29581 actaccatga tttttgccat aaaaaatccc tccatctttc ttcttgctac tacaatatca
    29641 aagaagccct tgtgaaacaa gttggcttac ctggaaaatg tcattttagc aaacgttaaa
    29701 aaaaactgga aatatgcgat ttaatcgcgt ttgtctcttc caagcagctt aaatttatta
    29761 tgttattctt aataattggt ctattatctg ttatttttct ttcgttgttt ttgtctttta
    29821 aattttcgat aacaccaata accgaaaagc gttagcacta gcaatatacc tagaaggagt
    29881 ataagtaatc gataaagatg gtattttttt gtctgttcta tctttgaaga gacttcttct
    29941 gtaaagggaa ctcgtcttcc tgtcactagt aatcggtgag tgttcacggt gtaaggtgta
    30001 caagtgataa gtgtgactag atcctgcttt tctctaattg ctaatgtatt tgtttttgtt
    30061 ggaaggacaa caattttctc aacgacttca taagcgagca tccgaccata cacttcaaga
    30121 tagaatcggt catcttgctc catttcttca agatccgtaa agagtttttt ctcagcaata
    30181 ccgctatggg cggtaatcac ggaatgtgta ctctctcctc cagttggaaa agaggtgcct
    30241 tgcaggactg tcgctcctct atctaacaaa agatcattcg tttcatcaaa taaaggcaag
    30301 ctaacattga ttttcggaat atagatggct ccgatcatat gttgttcgta atattctttt
    30361 cctggatttt ggacatttct aaccgcttgc tcaaaaggat ctttgacttg tcccattcct
    30421 gggattactg atgtatgagc aagcgtctga tttttctttt catgcactgc taattgttgc
    30481 tcatgttttt cttctttatc gcgttcaagc tgcttttgat aattctcgat ccttctttga
    30541 tccaaataat tgctgagagc atctgctaaa aatggatatg taaaaactgc agctccggca
    30601 agaaacatca ctgccataaa taattgtaat aaaagactga tttgggtttg aggtctatta
    30661 cctattttct ttttcatttt cttcaccttc ttatttaaaa aaactgcagc aacctttata
    30721 aaaccgattg ctgcagctta tttttgttgc ttatacttca gacttcattt tagatttacg
    30781 gtaccagctg tacgctccaa tcatcaaact tattccgatt agtaagaatg ccaagagccc
    30841 gttcccacca gtagcaggaa gtgacccgcg tttcatattc ggtactcttt ctggccctgg
    30901 attttctact cctacgatct gaatttcttc attatagctt ccataagaga tgataaattc
    30961 agtaaaggct gcctcacctg ttggaagtac ataaccttct ggcgccatgg tctctttcat
    31021 ttgataggtt ccataatcta acccttgaac ctcaaagtat ccatttccat ctgcgacaaa
    31081 ttctgttgct tgtccgcttg ttgcccactc aattctatct gctcctgatt cataaacagc
    31141 taaaggcgac tcccctttat aaaatattgc gtattcagaa cggtcttcat tccacaattc
    31201 gaatcttgca tctttaagca attcactgct tgatgcatca tatttttcaa actttcttcc
    31261 acctgttgtg actttctctg gcggctctac tccttcttca taatcaaatt catcatttcg
    31321 tccgatttca ataaccgctg tgttgttatg gaaatcatcc gcttctagtt ctgtggaaac
    31381 cttcaaatta tacgtgatcg ttaaatctcc tccacgtgca tctgctaatc tacctaaagt
    31441 atctactttg gctactccat tttcttctgt caaaatcgtc acgacaaacc cgttgcttac
    31501 tacttctact atataatctt gtccttttgt taaaatcgtc tcgccggctc gcacttctat
    31561 ttcctgatca taataagtta gaccaactgt aggcgtatct gtgattttgt aatttttgat
    31621 aacagcgctt ccgttttcaa ggacagaatc gatgaaataa ggaataggtg ctgtgatcgt
    31681 gtaagaagcc gtttttcctc tttctagatc aatataagaa atcgtttctt gttctccagc
    31741 ttcattaata atcgttactt gtctaaggtc gctttcctct acattcatct gtttatctgc
    31801 ttctctgatt tcgtttttcg gatacaaatg cacatctgta ttgtatgtgt ctttccatgt
    31861 agaaccatcc cacacgccat ccaccatatc aggcatcatc actggcatca taacaaccat
    31921 tggagacgcg attctggaaa tatgagctgg cgaataagtt tctaagaaaa gatagacttt
    31981 atctctagaa gaaggtgctt cttccgtcac ttggacggta gagaataccg cttctccagc
    32041 ttcattcgtt gtttgtttgt caatacttgt cagtcgatat tttaaaaacc aactttctgt
    32101 gtgccgtttt tgaatccatt caatcgcttc ggcagaatta agaccgtctt ctcgttccgg
    32161 tgttaaagga ttatctcgaa tcaaatcgta atatacttcc gttacgtcaa acaaatcaaa
    32221 atcgactcct ggaatatttt cttcaccaaa atccgtagaa accaatccgc tattcggtcg
    32281 ttcttccgga acagatgaga aacctttttt gtgtaatgta atcgtcactt cacttggttt
    32341 ttgtgcgact tcttcggctt tgactagttt tccgttcccg aaaatcccta ttcccgcagt
    32401 aacgagtaac agtacactca ttcccgtgac aatcattttt ttattcattt ttttctcccc
    32461 gttctttttt tattaataaa acagcaccta acaataaaag aattcctagt aaagaaatca
    32521 acgttcttgc ttcattcgtt tgcgggaata cggaccattt tcctgattga cctggattgc
    32581 ttggaccact cggctctttc ggtttgtccg ttgtttcttt tacggtctcg ttataaacac
    32641 gtggtgttgc ggattcatag gcagagactg gaactttccc ggattctaat tcatccatct
    32701 cctgtccgtt aactactaca tattgtggtt ggccttttgc atccacccaa aattcaggga
    32761 tgatgacctc gattctctta cttgcttcat ccatctcgta accttccaca ccttttaatt
    32821 cttcaaaata ataggtgcca gaaggcaaga aacgttgtcc tgtcgtaaca aggccctctt
    32881 tatccgaaat gaatttgctt acatttttgt cattcaaagg atcattgtct gctggtttga
    32941 tccattgatt tttcaagtca ttagcaggag ccatatctaa ataatatttc cccccttcta
    33001 tcatctgata tagagcaaaa acagcacctt ctaatggttt tcctttttca gttgttcctc
    33061 tttgacgtcc atacttgaag aagtatggat ctcttagata accgacattt ttaggataga
    33121 tatgaatctc ttgcagttcc tcgcttgatt ctgtcgggtg ataaattggc aaaatagcag
    33181 caattgggat agctgtctga tcgatatcaa catccaatcc aacttctgga tcaatcgatt
    33241 cttcgaaaat caaataaact gcatcccgat cttggttttt cttaggtgct ttgatacgtg
    33301 caatcccgtc tcctaatccg ccaaccgtat ctttcccagt tgttaccgaa gtccctattt
    33361 ggacaagcct ttcttctgtg attagctgac ggatattgtt tctgttcgtt tcagcgattt
    33421 gctggacgaa ttctttagga gtcatgtttt cagcagcttt tttttcatga taataatcgg
    33481 tcatgtcgta aatggcaaat gtcgctccat ttagtggcac cgtctgtgaa atgatatctg
    33541 ctgtttcccc tgcttcctca gagatagcca agccatcatt ttcatggtat tcaaattgat
    33601 cgttataatc gatatctcga atcatccgtt tatgcaacac gaattccacg acatcttccc
    33661 ctgattcttc tgccgacatc gcctgtggaa gaaggactgc tgcaagcggt agtaaaacaa
    33721 gtaattgaag catcaaacac aacagctttt tcattacctt gctccctcct ttttagattg
    33781 tctgacaaac aaaccatagg ctgctcctgc aagcgccatg aagaatgcag caagcaggat
    33841 atagaataat cgtccggatc ctcctgtagc aggcaacgga ttttcaggat cgttcccgac
    33901 actaagctgt aatctgattc gatttgtctc caaaagttca tattctaact cttgctgtag
    33961 atcaggcgtc tctgcttctt cgtttggata atacgaaatc tcaactttta gtgtacccgc
    34021 ttcttttcct ggaactaccc gatcattttc tatgtcaggt tcttctcgat aactaatttc
    34081 taaaataaag taacctggaa gttttctata gctgtcaggt gacttcactt ccttgatccg
    34141 atagacccct tccaacaagt ctgaagcatt gaatagggaa gaatcgtttg gatctggtgc
    34201 caagcctccc tcgatgattt catactctcc tgcactattt tgtttctcaa ttgaaaattc
    34261 tgcgccttca agacgtcggc tattcatact gtcaattttc aaaagttcca aatcgattgg
    34321 cgctagttca tcttctaagg ttacttgtag tacaccatcg atgatttcat agccttccgg
    34381 caagggagca gtttcgtctt cgatgaccgt atcttcaatg ataaacttac cttcttcatt
    34441 cagtgcaaac tggaacactt gatcagatag ccgataccca tttggtgcga cagattccga
    34501 gacctcatat tcaccaatag acagatgcag aggttggtcg taatttaaat cgacatactt
    34561 ttctactcct acggcacgag aatagaagta tccgatccca ggcaaacgtt tcgattcttc
    34621 tggttctaac ggattaccag tgtatgctcg atacagcggg aaggaagaat cccccacttt
    34681 cctgatatcg aaacctgcac catctaatgt atggccttca tggtctaatt ttctaactcg
    34741 gatctcccga taatcaaacg tattgtaagc agtgaatgct acccataaac gatcctcttc
    34801 atctgttgta tacgtgagat attgccaatt ttcttcacct gtttgcttga gttggatagt
    34861 ccctatagca ttatcgtaaa gtcgcaagat ccatgtgtca ttttcatcag gttctttgaa
    34921 accttctggg ttctggctct caatgatttt gtattctgca tagttggtag tttcttcact
    34981 atccttataa acaaatgaat ctggctctcc gattggatga ataactttct caccatccac
    35041 gattacatat ttcccgtctt catctactga atagaacgtg agtctccctt tctcttcctc
    35101 tacttcgttc acaccagaaa gccctttcgc tactggatat ccgatttcca gtccgttacc
    35161 atctgtttca taaagttcaa actctgcgcc attgattggc atattactgc cttcaattag
    35221 tttattgata ttcatgtgga ttggcttgaa ttcatttcgc atttccagtt tgacatacaa
    35281 ctcatcatac tctgtgcctt ctatgttagg ctcttgtaca acagaaagct tgtgatattc
    35341 tccttcataa taggaattcg gatctttacc ttccggtccg ccgactctcg aaccagatgt
    35401 atattcatct acaagctcgt agtgacctaa catctcatct tcgttggctg catatttttt
    35461 gatttggaat tgatacgtct catatactaa ctgataacgt ggtggtccag cgatctctct
    35521 gatccaaaat atgtcgctga ctgctccagt ttcatctcct aaaatcggca tcttcgtttc
    35581 aaagatcgct attccgtccg tggtagtttt acttgtttcg attggttcgg cttccccact
    35641 ctctggattt tggtcatata atgcaaattc gactccagcc aacggctcgc cattttgacc
    35701 cttcttgctg acttggaaat tacggccttt atgttcgtaa cgaatggaag aagatgggaa
    35761 atacatccgt ccagtagtga tatcattctc ataatcctcc aggttctgaa tataggccat
    35821 attgttcact gtgtaaaaat ttccgtcttg agctcccgca tcaagtttta cttggtactt
    35881 gatcctgacc atttcccctt cacctaaaga aatcggagaa aatttataag cttggttttc
    35941 ccgtccgaca tacgttgtcg gagtcgccac tacttctggt ggggcttgtc catgacgttc
    36001 ttcatcccat atttctggct cttggtactt catgccgttt tctcctggat ctagatggta
    36061 aaactgaata tctgccgatc tgccagctcc aaatggtgag acattaaaac cagtttcatc
    36121 ataaagtgtg gcttgctgga tcgttctctt gaaggtagaa gagatatcct ccaaggctgt
    36181 aacaatgtca ttcttgttgt tcgcattgta atatcgcggt gttacgccat cttcttctaa
    36241 atcagcaata tttttcaata catttcgtcc aatagcatct agtcgttgtc tcccgctaac
    36301 gtttccatcg attcctaaac caatggagaa gatacttggc gggttctctt gatacttgtt
    36361 tttcagccaa tgatgataag caactgtgtt gatcgcagat gacttttctt gcgaagtagg
    36421 actgccattc gtattgttat tagaagaacc atcaccaacc cagtgagtcg ctttattatc
    36481 tccttctttt acctctccat aacgatagtg tacatttact tcatcttctg gacggttaaa
    36541 gtcagatgca taggtaactg gatgagtaaa ccctgcatta gaagtattcc cagaaaactc
    36601 ctcaaaattt ctgaacaaaa cgtcattatc ttcagcaatc ttatcttcca tcacagtcca
    36661 gttgctccaa gctccattag tgctcgtacg ataacttgac tgaatcggtg cataactgaa
    36721 ggtcggtgtc ccatctccca caacgattag gattttttct ggatttctat tttctccgcc
    36781 attgtcttta tacaatgttt catagccatt tttaagccct agtgtaagcg gagttcctcc
    36841 gattggtgta cgatttaatg ctgtattctc acttcccgtc aaacgattga tatcatttgt
    36901 caatggaaag atgtccgaac tgatttgctc atggttattg atagctgtac tattatggta
    36961 aaaattcacc ataccgattc gaatatccag actcgggtca gagagcagat ctttactcat
    37021 ttcgtttact gcctcaatca acgcagcatt tttagtttga ctattggtcc cagcggttaa
    37081 ttcactcata ctcgctgact tatctagaac aaaaacgata tcgattggac tgatttcctt
    37141 tttctcgccc ccgatgacat ctagatagac attgtaaagg gttgttgcat cttcaggatg
    37201 tagatcagat gctgtgctgc ttggcagaac aggttctacc cattttttga tgtacgcccc
    37261 gttataatcc aagtaactgt tttcaaaatt ccgttcattt ccccaaagct gaacggtttg
    37321 agctggaacg tttctatctt cttcgcctgc agttgatcca ttattagtag catagtcgta
    37381 atttcgccag ttcgctcccc cgtttgtata atcactatca tcgatcaagt tctctccgtc
    37441 atatggcgag cctttgtctc cttgatagcg ttcattgctt tgtgtccaat ttcctaacac
    37501 ttttgtatag tcattcgttg taaatttcgg gtagcgatgc tgttcgtttt caacttctcc
    37561 gctaggatag atatattcat aggggtcttg cgtagctgtt ggcccaagac gctccaaaat
    37621 accgttgaat aactggcgtt gaatgcgctg gagtgcttga ccgctgggta cgattggttc
    37681 gtccagcttt tccatctcat tttcttcgct acgttgaatt tcagcggaat cttctgtatc
    37741 agtttcattt tcttgtaatt cttggttatc tattctttca tcaaccacca cttgaagagg
    37801 tagttcccct tcgttcacat cggggatctt ttctgtttca aaaatcaatg tgccggtttc
    37861 tgcttctttt gaataaactt ttccgacata ccattcttga ccatcgatcg tctcgacttt
    37921 taggtcttct ccatcttcat cataggagga tagacctgtt ccacgaacgt tttttacggt
    37981 accaatcccg gcggctgccg catcgattgc taaacgtatt ctaccttcat tggcactttc
    38041 ctttttttca aaagccagat gccattcaag ggtttcttct ttttcaatat acttcccttc
    38101 aatttttgca tagtcctcat ctaagaatac ctcatcctcc tttgtttctg ttgactcagc
    38161 agctacatcc agggtcgtca tatgatagac tgactggaaa tatggtccaa atagcaaact
    38221 gacagttact aactgcaaaa gcttcaattt taaccctttc acatccttat actcctttca
    38281 aaaatatttg tcttctttca caacaatcaa caaaacaatg cccatctaat tataatttaa
    38341 tttaatttta attttaaatc aaatgatatc ctttttagtt aaaaaacacc aattatacaa
    38401 catttattat taaaattaaa ttataaaatt ttataacgtt ctcatttaac aaaacttaag
    38461 ttattaagac aaatgaataa acaactacgc tttcctcgaa acaaaaaaat aaagcacaaa
    38521 tgcgcattta cgcactcatg ctttattcgt acaattaatt cagataacgg cagcgcatag
    38581 gatgccaata acacttccta aaatagctcc tcctaaaatg tcagtaggat aatgtaaaag
    38641 taaaaaaatc ctcgaataag cgatcaaaca agcaaataaa aaggctaata ttcctgcaac
    38701 tggacttaat tcgaacaacg cagctactga taaaaaggct agcgctgtat gtcctgaagg
    38761 aaaagaagaa gtcttcggtt tacttgtgta aaggacaagc cgacgatttt tttcataagg
    38821 tcttttccgt cttgtcgttt ttttgataaa aagcaaacca acaaaaaaat agatcaaact
    38881 gccgataaga atggctagac catattctct agtatcaggt atcaagacta aaacagcagc
    38941 aaggagtaac caaataaaac caccattaga gatattactg ataaatttca aaaaattgat
    39001 ttttcgacta tccgttttcc gttgcttggc gaacttgttg ataatactat catcgatttt
    39061 agcaatcaaa cgcaacatgt tattcctcct ttttaagaac cttttcttgc gtacgatgga
    39121 taaaaagcag gacttgaaaa taaaacaaga aactgaaacc taataaagca aaatagattg
    39181 gtcctgcaaa ccagaatgcg acaagaagga tcaacgtgcc tgccaaatag aaaagcgcgc
    39241 ttttgaaatc attaaaaaac aagaaaaatg cagtaatcca agaagcttct ttttgttcca
    39301 gttgaagcaa gattttgtaa ctaaagagaa gcgcgcttaa tagaagcaaa aacaacgtag
    39361 tatacatcaa tgtatagcca attcccccac cccaatagcg gaaaatctga taatcgatca
    39421 atagaagaag aaaataaaca cttgctaaac tatcttgctt tccataaaga gagaagttct
    39481 cacgaaactt tttccagaat agtgaaccga gctttttctc ttcatttttt ttgtaatgat
    39541 aaatcatata cagaactgtt cgacttgcag ttcctaatgt gaaaacaggc aagacgcaca
    39601 ggaaccatag tagctgtaaa tggataagac gtggaatcca ttcaatgatt tctaagaaat
    39661 aatcattggc ttttttacca atcgatcgat tcatcatcct tttgaacctc ctgtcaaatc
    39721 catcccttca atgaaataac gttgtgcaaa aaagaacagc aaaatcgtcg ggatcaagac
    39781 aagtgttgct cctgccatca gataattcca ttgtgtcgtt gcttggttct gaaaaacttg
    39841 caagccaatc tgtaacgtgt atttttcttg atcttggata tataacagag gacctaagaa
    39901 atcattccaa gctgcgttga aggaattgat cgcaatggta atcaaggcag gtttcgtcaa
    39961 tggaagcatc aaatgactcc aaatatacaa atgattcgct ccgtcgattt cagcagcttc
    40021 gatcaattca ttattgatcg ataagtaaaa ttgacgcatc agaaatatat tgaacgcatt
    40081 gccgaagaac gatggaacga tcaggggcaa ataagtacct acccagccga tcttggagaa
    40141 taatacatat tgagggatca tcgttacaaa ccccgggatc atcatcgtag aaagaaccaa
    40201 agcaaacatc agctttttcc caggaaaatc aagcttcgca aatccataag caataaaaga
    40261 atttgaaagg acattaccga tcactactag cacagtgatg aataatgtat ttcttgcata
    40321 gcgccaaaaa ggaaaggcgg caatcgtatc cagatagttt tcccatacga ttttttctgg
    40381 gaaaaaggtc ggaggataac gaacgatttc ttccattggt ttcaatgaag taaacaccat
    40441 ccaaagcaat ggaatgatca aaatgattcc tattactgtc atgaggacat aacttagtat
    40501 tttttttgtt tgccgattca cactggtctt tttgaaataa tccatcattt gccgtcacct
    40561 ccagaataat agacccactt aggtgcaagc ttcaaattga caaacgtcag aaccaacacg
    40621 atcagaaaca ggatcattga catagccatc gcataaccca tatcaaaagc tacgaacgcc
    40681 ttgttccaca tatgcagatt ataaaaaagc aaagaatttg caggtccgcc tgttccattt
    40741 ttcgtcatga cataagcttc ttgaaaaatt tggaaagaac cgatcgtaga ggtgatcaca
    40801 tcgtaaaaaa taataggagt gattaatggc aaagtaatac ttttgaactt tccccaaacg
    40861 cctgctccat cgatttctgc tgattcatat aaagatctag gaacattttg taatgtagct
    40921 aagtaaagta gcattgctcc tccagaactc catattttca tcaaaatcaa cgcaggtttc
    40981 gtccattgtg gatcaaacag ccaagctggt ccagggatat ggaaccagcc cagaaatgtg
    41041 ttgatcaatc ctgcagatgg agaaagcagc tgcatccata agatgtagac tgctactcct
    41101 gataagactg ccggtaaata gaaagctgta cggaatacac tgattccttt gattttttga
    41161 ttgagtactg tggctaaaaa cacactgaaa atcgtcgtca gcggtacatt gaagatgacg
    41221 aaataaacgg tgttgtataa cgatttccaa aataaatcat cgtgaacgaa cattcttttg
    41281 aaatttgcta gaccaatgaa attcatcttg gatgtcaaat tataatcggt gaaactacca
    41341 ataactgaaa aaagcatagg ccctagcata aataccaaga acccgataat aaaaggcgag
    41401 ataaataaat aggcccatct tgcttctttt ttctttcgga tagataaacg cttcaaattt
    41461 tcttgcctcc tctgtaccta gacagtcaaa aatggattat tgattttgct tgactaagtt
    41521 ttctactgct ttttgtgcat ctgccaatcc ttcttttggt gattttgttc ctaaaagaat
    41581 ctggtcaatc tgttcattca ctaatgtcgt gtaatccgga gcatctaaag gaactggtgt
    41641 gatcaccgta tttttaaagt tttcgtccgc catttgatag atcatttttc ctttatccgt
    41701 taggttgtca ttattcacta aattctcatt ggctgtcttg ctcgccatga tatcaaaact
    41761 tttttcgccg aatttttctt gtacttctgg agaagaaaga tatttgatga attcataaga
    41821 ggcctcagga tcttttgctc cgtatggtac ttccaagacg aatccgccac cccacgacca
    41881 atggcctgaa ccttcttctt tttctgggag ctgcatcacg ccaaaatcaa aatcatccgg
    41941 tgcattttcc atcaaactag agtaataatt gatattttgg gcacgcatgg ctactaatcc
    42001 ggaaataaat ggatcagata caccagaccc aaattcggct tctaatttat tgatcgtatt
    42061 tttcccataa taatcttgcc agtcttggat ccactccaag gcttccactt tatttttagt
    42121 atcaatcttg actttttcat ctttgtcaaa ccaactagtc ccgttatctg cgtttagcgc
    42181 ccatacatca gcccctaaat tccataatgg ataaaaaccg atgcgtgtaa attcttttcc
    42241 ttctttttta tctagtttac gtgctgtttc ttctaattca tcccaagttg tcggtagatc
    42301 ttgttcactg attccagctt ctttaaataa ctttttatta taaaaaatga cttgtgtata
    42361 tgtattgaat gggatcgcat aaggatcacc ttcatattct actgtatccc aaagctgcgg
    42421 ataaaaatcc ttagaagtgt ctttttctac atatttggag agattagttg cttgctgtgc
    42481 ttctgctctt tgggcaacgg aatttatgtc ctgtacaata acatcgggtg catttccagc
    42541 tgttattgca gataaagatt tagtccagat gtcaccccat ggctgatact tataatttac
    42601 atggatctcg ttttgtgatt gattgaaatc ctcaatgatt tcttcgatag cttcttttcg
    42661 tgcaccggat ccccaaaagg accagaaatc aatatttttt cttccatcac ttgttttttc
    42721 agaagaattt gctgcacttg aactggcatc gcttgcatta cttgatccgc atcctcctaa
    42781 taaaaacaat gctgaacttg ccagcaaccc tgtaaacaat aattttttca tattttacct
    42841 ccaaatttta atgtaaccga tgtcggtttt aatataacga tatcattttg ttctgaaaat
    42901 aacaatttat aaggtatgat tctaaaaaaa ttattaatga taccaatact ttatataatt
    42961 cgcaagcgtt ttctttgaat tttttaatct ttcgtagcag aatgagaatg tattaaaaat
    43021 agattaggag gaattttgat gtttagacac cagaaagaat tgcagtttga agcaaaacca
    43081 gatcgtccga atccattaat cgcgaaatat ctgcaggagc tgatcggcgg ccaatatggc
    43141 gagatgagtg tagctatgca gtacctgttc caaggctgga gctgccgagg agaagaaaaa
    43201 tataaagata tgctgatgga cattgcaaca gaagaaatcg gccacgtgga aatgttggcg
    43261 acaatggttg ctcgtttatt agagggtgcg cctgttgaag atcaagaagc agcgatgcaa
    43321 aaagatccaa caatcggtgc cattatctct ggaatgaatc cacagcacgc gatcgtagct
    43381 ggcttaggac ctcgtcctgc tgacagcgtg ggcaatccat ggagcggtgc ctatatcatt
    43441 gcgagtggca acttgttagc agactttaga gctaatttaa atgccgaatc acaaggaaga
    43501 cttcaagtag cacgtattta cgaaatgatc gatgatcgtg gcgtaaaaga tatgctgtct
    43561 gttctccttg caagagatag ctatcatcaa aatctatggg aatgtgcagt gaaagaatta
    43621 gaagaaaaag aaggttcaat cttagtccca agtactttcc cgcgtgacaa agaacgaaaa
    43681 gacattgctt atgacttcta taacttctca gagggagacc aaagtagtga aggttcttgg
    43741 gctagtggca aagcactgga tggcgaaggc gaatatcaat ggttgaaaga acctaagatc
    43801 gaaggcgctg caccaaaatt agaaccagta acaccaaaaa tgtatgggac acctccattc
    43861 aagaaataaa tatctacccg tagaaacacc cgttttttgt tccacacgct ttgtgagcaa
    43921 aactgactaa agtcattaat cataaccacc cttcaaaagg gtggttttct ctagagctat
    43981 aagctcaaat caccggccag cgcctaaaga cgctggcttt catattttat gttcaagcca
    44041 tattgccttt gccagttttg cccacctctt aaaaggttta tgtactactc cttagccctt
    44101 aaaaggatcg tcttactctc gtacactcag cttatctaac gcactatcat gtttttcttg
    44161 ttcttgaatg tactttttga tggtcgcttc attttgtcct acttttgtca catagtatcc
    44221 ttccacccaa aagtgtcgat acccaaattt atattttaga tttgcacaat ctcttgatga
    44281 tctcctgaat attggctcga tattgattat aaattatttt tcttctatac tttggtgtga
    44341 atacaatgtg gtatttacac atctactttg tacgggctaa actatttccc tttattgctc
    44401 cacccgcata gcaagtggtt ttgtatttca atcgctcggc gcgctcaatt aggtctaaag
    44461 acaataaatc taaaaacccg ctgcttcact gacagaaaca acgggttttc agaaaaaaat
    44521 gaaatgaaaa agatctctta cggcaatcta ttttattttt ttgatttttt ataaatggaa
    44581 aatgcttcaa gaagtccaag aaataaagcc gtataaaaac cagaacctat gaaatcttta
    44641 tttattagat attcaacaga aatcccgata acactagcca acaatatatt tatcagaagt
    44701 gtaaaataag gatgcttttt atagaataga actagtgttg ttttcataga ctctcctttc
    44761 ttcatatttc caattaatca caaattcacg gcattactag aaaaattaaa ggggcgcaga
    44821 agtggaaaaa tactcttcga ctttccctac aaaccaggcc atcttgtgtt taataactat
    44881 accaaatcta aggtacctct aaatctaaat tccatttgtg gcggtgacct ccaattttta
    44941 actcttctat ctcccatcca gtatacataa ccttgatact tcgaccactt atatatcgaa
    45001 cacacaaata cctatccatt gcaggagtac tacctaactt agttgctact tgcatctaaa
    45061 taacagctga aaagatagga cttactctta gtaaagtatc tgaactatta atttttaaac
    45121 tatcattttt atcctcaact acatgcacat ctgttgcaac ttcactttct gtctcatcca
    45181 gataaatgat taaggaatcg ggaattgcaa tatcttcagc tgacgctgga atagttgagc
    45241 taaattagtc atcatatctt tattataaaa taaccaacag caaaaaatac aatgaaaaac
    45301 aaacttggaa ctaatgtatc attaataaat gttgaaaaat tcagcaactc attactgtat
    45361 gattttagca gcccaaccaa catataccct ataaaagcta ataagatggt taggaatcct
    45421 ttagaatttt tactcattca tagacacttc cttaaataac ttttattata gaaggtattg
    45481 taaataataa tattgacaaa aagtgactca aaataaaaaa accttacatt atactggaat
    45541 gaactgtatt gatagtgtac ctaaaagctc tactgacata agatcgtgta atcggtaaag
    45601 taattttatt aattatacgg gatgtaatca attagagaat aaactaatca taaaaggtac
    45661 ttttacattc ataattgtcg ctttgagatt ctactttggt tcactatctg acccaattgc
    45721 acattttcga tcattacaag tattggtgtc gttagaacaa ctaaataaaa actagcggta
    45781 gatttttcag aatctatgct aatttctgta tggatgttcc agtgatggca aatttctctt
    45841 ttcattgttt ttcttgtcgc atgatatgcc tttttgtgtt attcagttaa actatagaaa
    45901 ttctttccta gacagatctt gatcccaaat tttacgtttt tgagcatcga tatagtttaa
    45961 aatgacatta ctcatatcta aagagtttag attttttagg actaaaactg tcagttgttc
    46021 tttttcttct ttctgttaaa gtattgtcgc gacttttcgt atacgttaag atatgtcgtg
    46081 aaaaaacaag ttttgtgaca caaaaaaacg aatgacttga ggaaatcacc tcaatcattc
    46141 gtttaaaatg ctatatattc tttagtttaa gatacggaaa ttaatgccat cgaccacact
    46201 agtagaatga acgacattta cttggccttt ttttaatttc gttatgttta gcatataaaa
    46261 atattataga gtttacttac aatgcttaaa acatatactg tataatctga tcaattttag
    46321 ccatgtcttg caatggtaag ttttctattt ttttgagttt tcgttcctta aaatcaagag
    46381 attttaattg tgtaataatt acttgtcctt ttatatctag ctcttcaggt aatgagtaac
    46441 gtgtcggtaa attctttata gtggaagtaa ttggacatat gaccgaaaac attgttttac
    46501 ggttaaattc ataacgagag acgactagtc ctggtcgtct tttttgaatt tcttttccta
    46561 cggagggatc aaaatcaatc catacaatat cacctttttt aggaatataa attctttctt
    46621 ctgacactat aaaatttctc tcccttcagg agttaaatct tcccattcgt ctttctcata
    46681 atactcggcc tcttccccac cactaaatgg atcttcaatt tttggaacta acgtaattgt
    46741 tccatcatca gaatacacaa cgatatattc ttggttttct gaaggtttct ttccgttatc
    46801 agaaggtaaa gttaccacta cagaacttcc ttgtaatcta gattttgtaa caagcattat
    46861 catcactccc ttaataaaag tatactatat aacactaagt aacgcaaaat tcccctttgt
    46921 aaactatcta gaaaattgtt ttgtttaaag tatatcttat tggatataaa tagaaataaa
    46981 ttgtagccag ttcatttttt ctttaactaa aaaaataatc caagaaacat tgaacctatg
    47041 gatccaaaag ctacccaaat actatagcta accccccgct accttcaatt ttgcagataa
    47101 aacgctttgg gagaaaattt caaagacaac aggccatcct atcatgcccg attataaaca
    47161 aaaagctggc tattcctttt gctctgacaa atttcaattg ggaaagttca cgaaactagc
    47221 aggtgaagaa gtttcaactg ttcggatcaa agaatgcccg cttcaaatag aaacattggt
    47281 tcaacgaatt tcaactagac aagactttgc tattgtagaa cgtgagatcg tagctatttt
    47341 tgtagaagaa gggattttac atgatccaac tcatatcaat gtggataaat ggcaacctct
    47401 tatctataaa ttcagagaat atacaactgc caaccagtcg ttaactttga attttagatt
    47461 ccaagaattc aaagagtcat aatgataaac atcgttaggt ataaataaaa aagactcgca
    47521 gcacgagtaa aaaacaaata gaagctatac attagaaagg tcgactgtga ccctatagat
    47581 gcagtatatg cagcttatga gcatttcttc tgttctcttt gtttttggga tctgtgctga
    47641 agtctttttc gttttttgcc gctctttctt tgaaaaatcg attatgttat accttctcca
    47701 ttactaattc aggcatctgc tgagtaatga tttttcgcgc aaaagaaata tctgttaccc
    47761 aaggttcgat ttgttcttca gtcaagacaa gcggcattcg gtcatggatt ggtgcgactg
    47821 tgcggttagg ttcagtagtc attatgattg attctgccat tttttcatcc tgatggctac
    47881 gataaaaacc gccaatatac actatttttt ccttatttga aaacataaat ttgtcttttt
    47941 cttcattcca ctcataaaag ccactcattg ggaaaacaca acgattttgc aaaaatggtt
    48001 gatgaaaggt ctttttttct tctactgtct ctgcacgggc attaatcatc aattgtcctt
    48061 ttttaaatcc ggtaaatccc cacttcatga tttccggagt gaggatgttt tcttcctctt
    48121 ttcttaacgt cacaacaaga tttgacggaa aaacttcccc agctgctaat tctgctgcct
    48181 gcttcggttg tttcgtctgg gcttctcgat aataattctg aaggagctct gactctagat
    48241 caaaaaaata acgaccacac atcctaatcc ctcccaatct ttttttctat catacactgc
    48301 aacaagaagt tcgcaccaac aaaacgcttt caatttttgt agaactaaaa aagttgtgac
    48361 aagtgacaaa ctctccagtg aatggtattc tcctgccaac tcctcggtca caagtcacaa
    48421 tattcaacaa acatgagaag ctgctttttg aaattgttcc ttgtgactcg gagtgaaacg
    48481 gcaggcttac gacctctggg taaacggtgt tcaaaagctt gcatctgcgg taataagccc
    48541 aaccaaacga aaaatatgag cagctatttt tatttggctg ttcttattac ttgatgcagg
    48601 gcagcttttt cacaacctct ccactaaacg gtgttcaatc agctaacctt cggtattagc
    48661 tcaaaaaacg gcaaaatatg aggaactatt ttgccgtttg tttctctaat actcaggtca
    48721 aagcgctgat ttcacaacct ctaatacacg gtgttccaga agtagcttct tcga
//
LOCUS       DACDYI010000012        48184 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.012, whole genome shotgun sequence.
ACCESSION   DACDYI010000012 DACDYI010000000
VERSION     DACDYI010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.012"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            85..972
                     /locus_tag="IWM52_000659"
     CDS             85..972
                     /locus_tag="IWM52_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9972935.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="IWM52_000660"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="IWM52_000660"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAP9972936.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="IWM52_000661"
     CDS             2274..2720
                     /locus_tag="IWM52_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP9972937.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="IWM52_000662"
     CDS             2993..4345
                     /locus_tag="IWM52_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAP9972938.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="IWM52_000663"
     CDS             4516..4803
                     /locus_tag="IWM52_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972939.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            5029..5472
                     /locus_tag="IWM52_000664"
     CDS             5029..5472
                     /locus_tag="IWM52_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9972940.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(5478..6662)
                     /locus_tag="IWM52_000665"
     CDS             complement(5478..6662)
                     /locus_tag="IWM52_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAP9972941.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            6930..7628
                     /locus_tag="IWM52_000666"
     CDS             6930..7628
                     /locus_tag="IWM52_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAP9972942.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            7635..8312
                     /gene="nth"
                     /locus_tag="IWM52_000667"
     CDS             7635..8312
                     /gene="nth"
                     /locus_tag="IWM52_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAP9972943.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(8438..8971)
                     /locus_tag="IWM52_000668"
     CDS             complement(8438..8971)
                     /locus_tag="IWM52_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972944.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(9268..11649)
                     /locus_tag="IWM52_000669"
     CDS             complement(9268..11649)
                     /locus_tag="IWM52_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP9972945.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(11639..12286)
                     /gene="recU"
                     /locus_tag="IWM52_000670"
     CDS             complement(11639..12286)
                     /gene="recU"
                     /locus_tag="IWM52_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAP9972946.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            12350..12889
                     /locus_tag="IWM52_000671"
     CDS             12350..12889
                     /locus_tag="IWM52_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAP9972947.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            12979..13398
                     /gene="gpsB"
                     /locus_tag="IWM52_000672"
     CDS             12979..13398
                     /gene="gpsB"
                     /locus_tag="IWM52_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAP9972948.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            13420..13787
                     /gene="rnpB"
                     /locus_tag="IWM52_000673"
     ncRNA           13420..13787
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IWM52_000673"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13996..15162
                     /locus_tag="IWM52_000674"
     CDS             13996..15162
                     /locus_tag="IWM52_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAP9972949.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            15208..16704
                     /locus_tag="IWM52_000675"
     CDS             15208..16704
                     /locus_tag="IWM52_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAP9972950.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            16785..17468
                     /locus_tag="IWM52_000676"
     CDS             16785..17468
                     /locus_tag="IWM52_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9972951.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            18113..19303
                     /locus_tag="IWM52_000677"
     CDS             18113..19303
                     /locus_tag="IWM52_000677"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAP9972952.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            19405..20358
                     /locus_tag="IWM52_000678"
     CDS             19405..20358
                     /locus_tag="IWM52_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAP9972953.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            20427..21776
                     /locus_tag="IWM52_000679"
     CDS             20427..21776
                     /locus_tag="IWM52_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9972954.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(21959..22282)
                     /locus_tag="IWM52_000680"
     CDS             complement(21959..22282)
                     /locus_tag="IWM52_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972955.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            22411..23574
                     /locus_tag="IWM52_000681"
     CDS             22411..23574
                     /locus_tag="IWM52_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972956.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            23644..24993
                     /locus_tag="IWM52_000682"
     CDS             23644..24993
                     /locus_tag="IWM52_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP9972957.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(25038..25469)
                     /locus_tag="IWM52_000683"
     CDS             complement(25038..25469)
                     /locus_tag="IWM52_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9972958.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            25857..28499
                     /gene="alaS"
                     /locus_tag="IWM52_000684"
     CDS             25857..28499
                     /gene="alaS"
                     /locus_tag="IWM52_000684"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAP9972959.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            28699..29295
                     /locus_tag="IWM52_000685"
     CDS             28699..29295
                     /locus_tag="IWM52_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9972960.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            29417..30565
                     /locus_tag="IWM52_000686"
     CDS             29417..30565
                     /locus_tag="IWM52_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP9972961.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(30603..31016)
                     /locus_tag="IWM52_000687"
     CDS             complement(30603..31016)
                     /locus_tag="IWM52_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAP9972962.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            31095..31526
                     /locus_tag="IWM52_000688"
     CDS             31095..31526
                     /locus_tag="IWM52_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAP9972963.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(31625..31858)
                     /locus_tag="IWM52_000689"
     CDS             complement(31625..31858)
                     /locus_tag="IWM52_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAP9972964.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            32041..33711
                     /locus_tag="IWM52_000690"
     CDS             32041..33711
                     /locus_tag="IWM52_000690"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAP9972965.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            33858..34244
                     /locus_tag="IWM52_000691"
     CDS             33858..34244
                     /locus_tag="IWM52_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972966.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            34231..34929
                     /locus_tag="IWM52_000692"
     CDS             34231..34929
                     /locus_tag="IWM52_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9972967.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            34934..35398
                     /locus_tag="IWM52_000693"
     CDS             34934..35398
                     /locus_tag="IWM52_000693"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAP9972968.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            35395..36306
                     /locus_tag="IWM52_000694"
     CDS             35395..36306
                     /locus_tag="IWM52_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP9972969.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            36541..37080
                     /gene="pyrR"
                     /locus_tag="IWM52_000695"
     CDS             36541..37080
                     /gene="pyrR"
                     /locus_tag="IWM52_000695"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAP9972970.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            37094..38377
                     /locus_tag="IWM52_000696"
     CDS             37094..38377
                     /locus_tag="IWM52_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAP9972971.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            38390..39316
                     /locus_tag="IWM52_000697"
     CDS             38390..39316
                     /locus_tag="IWM52_000697"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAP9972972.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            39335..40615
                     /locus_tag="IWM52_000698"
     CDS             39335..40615
                     /locus_tag="IWM52_000698"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAP9972973.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            40620..41696
                     /gene="carA"
                     /locus_tag="IWM52_000699"
     CDS             40620..41696
                     /gene="carA"
                     /locus_tag="IWM52_000699"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAP9972974.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            41701..44886
                     /gene="carB"
                     /locus_tag="IWM52_000700"
     CDS             41701..44886
                     /gene="carB"
                     /locus_tag="IWM52_000700"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAP9972975.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            44940..45716
                     /locus_tag="IWM52_000701"
     CDS             44940..45716
                     /locus_tag="IWM52_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAP9972976.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            45713..46645
                     /locus_tag="IWM52_000702"
     CDS             45713..46645
                     /locus_tag="IWM52_000702"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAP9972977.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            46642..47358
                     /gene="pyrF"
                     /locus_tag="IWM52_000703"
     CDS             46642..47358
                     /gene="pyrF"
                     /locus_tag="IWM52_000703"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAP9972978.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            47365..47994
                     /locus_tag="IWM52_000704"
     CDS             47365..47994
                     /locus_tag="IWM52_000704"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAP9972979.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            47982..>48184
                     /locus_tag="IWM52_000705"
     CDS             47982..>48184
                     /locus_tag="IWM52_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972980.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRI"
BASE COUNT        15864 a         8393 c        10354 g        13573 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt tttcttggtt tcagattggg ttttcctaca aaatattttt atctttgttg
     4981 aataggaaac aaaaaaacaa tataatgaaa gaaaaagaag gtgtagaaat ggataaaaga
     5041 caactgctaa tagaagaata tctggattta gctaatgaga ttattcatcg taataaacca
     5101 gaaatggaaa aagaatttgc aggatttact ctaaatgaac tagaagtgat tgaacatatc
     5161 gggaaaatcc ctgatcctaa cgtgacaaaa ttagcgaatg ctagttacat gactagagga
     5221 gccatcagta agctaacaaa aaaactgata gctagaggga tcataaacac ttatcaaagc
     5281 gaagagaata aaaaagaaat ctattttcaa ttgacgccaa tcggacaaaa aataaggaat
     5341 agacacgagc aactccatca aaaatgggta cagcgagatg ctactgtatt tgaaaatatg
     5401 tcaaaagaag aatttgacac agtatttcgt tttatcggtc gatacagaga atttatacga
     5461 agtagcaaat gaagctacta ttttttattg tagtgataat aggcaaaaat cataagcaaa
     5521 aatccgaata ccgtcggaag aaatggtgct gcagcataaa gctgtcctgc gattagcggg
     5581 ccgatgacgc gcgatagaga ttgtatactt tgggtaccgc ccattacaag accttgctgg
     5641 ttatctgaga cagaattgga tagttggcca ttgaatacag gagtaaaaat agaatcaccg
     5701 aaaccataac agataatccc aaataaaaac aaaatcggca gtagaagaat tccagataat
     5761 gtgatgaata gataggcgag gatttcactg aacatcccta ttcttgtgat ctgctgttct
     5821 gaaaattttt tgagcaatcg aggcataatg aacaattgcg agacagtatc caagattcca
     5881 atcaaagaga agcttaaacc aaccaaccca gccttccact gaaaggcgtc aatcgaaaat
     5941 tgagaaaaga tagattgaag agagccagct gccaaccaaa cagcaaaacc tgctgtcaat
     6001 aaaggaataa cggatgaaat cttgaataat tgttgtaatt ggtgaaaagg tcttacatgg
     6061 gagaaactta aatctactga acgttttttt attggaaggc tttctagcat aaatgtgtag
     6121 ccataaacgg catttaagaa agtaaagagt gctccaataa atataggaac agaattcccc
     6181 aactcagcta atagaccccc tataatagga cccagcgttg ttcctatacc gactaaagca
     6241 cccatccatc caaaaacttt ggttctttca ttaggttccg tgatgtcggc aaagtaagca
     6301 tataaggtgg agatttctcc tgccgtcaat ccttcaatga tacgaccaag gaacaacatc
     6361 catatcgaat ttcctaaacc aaaaagcaaa tatccgatac ttgatcctaa taaactgata
     6421 ataaggatcg gatgacgccc aaaatgatca cttaagctac cgagaatggg ggcagataag
     6481 aaggcagcta gagcataaac agacatgagc aaagtgatat agaaagcttg ctgatgtatg
     6541 gagtgagaat aaggcagcgt taaaaacgga agtacaggac tgacgatagt aaatcctaat
     6601 ccagtgaata agacagagac gtatccaaaa actttagaat gtttatttgt gaaactgtac
     6661 atgtaaaaac tcctttatgc aataatgtta acttggaaac aaaaatagtg tatagtaatt
     6721 ttgtttcctt gtcaacattc taaggaacag cgaaatctac ttaatttatt tattccgaaa
     6781 agtgaagata cgcatcaaaa acatgaacaa acaaaaatca agtagacgac attgtaaatc
     6841 tgaccgatca gtgaagtaaa tagaaaagaa acctgttctt gcttcccatg ttttttttac
     6901 gtatactgtg attgagcgga ggagaaatca tgttgaattt agaaaattac ttaagcgctg
     6961 gacaaacagt catatctaat cttttactta aaaattatca taggatcggt ctacaatctg
     7021 aagaattttt atttgttcta cagttgcata tggcacagct tgaaggagat actttccctg
     7081 atttgcagat gattgctatg gatatgggaa tcaaacaaga tcagattttt caaatactgg
     7141 atcgtctcgt aacaaatgga tttatcaaga tagaaacgac gattgtcaac ggtaaaaaag
     7201 cagatcgata taatctttac cccatctatg atctattagg tgagtattta aaaacacaag
     7261 aaaaaaaata cgaagaaaaa acacaagaaa aagaaatcca gtcagtgtat caattatttg
     7321 aacaagaatt tggacgcccg ctttcttcga tcgagttcca gcggatcgga caatggctag
     7381 aagaagacca ttatcagccg gaaattttga agctagcact gcgggaagcc gtattaaatc
     7441 aggcttatag ttttaattac gttgaccgca ttttgctatc gtgggaaaga aaaaatctac
     7501 gtacaaaaca acaagtggaa gaagaccaaa aacgaagaaa acagcagctt cttcaaagag
     7561 aagtagaaca gtcagctcaa caagaagaat tgccgaaagt ctcactgaaa aattggttgg
     7621 aggagtagcg gaatatgtta agcaaacaaa aaacaatgga agctctagaa acgatgtatg
     7681 gaatgtttcc cgaagcacat ggagaactca agcacaataa tccatttgag ttattgattg
     7741 cagtgatctt aagtgcccag gcgacagatg tatcagtgaa taaagcaacg ccagatttgt
     7801 ttgcctcttt tccgacaccc gatgcgttag cagaagcttc aatcgatgaa atcattctta
     7861 aaataaaaac gattggtttg tatcgaaata aagcaaaaaa catcaaagcg tgtgcacagc
     7921 agctgattga acgtttcgat gggcaagtgc caacgtcaag agaagaattg atgtctcttc
     7981 ctggtgtagg cagaaagaca gcaaatgtcg tattggggga tgcattcggg ataccagcaa
     8041 tcgcagtaga tacccacgtg gaacgtgtct caaaacgcct gaggatctgc aaactagatg
     8101 ctacggtcat ggaagtagaa gaaacactga tgcggaaagt accacaagaa ttatgggtaa
     8161 agacacatca tacactgatt ttttttggta gatatcattg cacggcaaga aatcccaagt
     8221 gcgaggtatg tcctttgcta tcaatatgtc aagatgggaa aaatcggatg cgtctaaaag
     8281 aaaaggcgct aaaaaagaaa ccacgcctgt agacgtggtt tctttaattg caaataaatg
     8341 tatgaattat cttgttatta tggagaaaag ctgtttcgtt tatctttttt gcatgaaaaa
     8401 gtaaatgtac atactcagta gctaggatgg taacttatta caatttattg cgataaaaat
     8461 aaaagttgtt atttttattg ggaaaatacc cgatgtagaa aataatgcct tccaatatac
     8521 ctataaaata agtccagtat tccaaaggat tatataaata agtgacgtat ctatctaaag
     8581 gtgtaataaa aatgataagt gataataaaa aaataccatt tataactaaa cataaactaa
     8641 tggatttggg cggtttcagg ctcatcagaa aaatatagat tctggtgaac aacaaccata
     8701 aaaaagtgag aatcattgca tgagagcttc tttccataaa ttttccttta agagtagtgc
     8761 ttaaaagtac agaaaaaata ataaatgcaa ggataggagc aattaaagta aatttattta
     8821 ataataaaca aaatttttca gcttttttat tcttggaata acgaggtatt gttttcgata
     8881 aaaaaagatg gtgcagacaa attatgataa ttaagcatac tatcagataa ctcaaaatga
     8941 agccttcttt caaaaaatta ttcaaaacca tatattgtat tttttatttg ataataacaa
     9001 tgggattatt aagaagtggc actaaataaa agactagtgt gtatttagcc gatttaatta
     9061 taaacaagta aatggataaa gtatatgtaa tgataaaaga aaaatggatt ttctaatttt
     9121 taagctaaat gttagatcac acatgtatat gctggatagt cttataaaaa atcatgtaat
     9181 acagcttggt acatgatttt tatttgtagc tgttttttac atagacacaa aaaagaaacc
     9241 acgcttgtag acgtggtttc ttttttacta tctgtttcca gaagctgagg tgctgctgga
     9301 tggtgtatgg tcatttgcag catccgctcc gctagttgct ggctcactgg aagaagcagg
     9361 aggagcagat gaagacggtg tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga
     9421 agacgctggc tcgcttgttt ctgctgtcgt gctttctttt gaggactcag cagttgattc
     9481 gctttgacta gtagagctag agctttgagt cgttgtctct gttgttgagc ttccaggagt
     9541 ctggacatag ctggaagatg gaatagttgt tgatggtgta attgcattcg atctagctgt
     9601 atattgatct ttgtagtaca gttcaccgcc cacacgaatt aatccgcttg gcatctccca
     9661 gtctgtattt gtcacgttag ctgaaacata ctgcataagc tcgcgataaa catcagaggc
     9721 tacgtgtgac gattctgaag tgacaggtgt cattttttta ttgtaacctg tccacacgga
     9781 aatcgaataa tttggcgtat agcccgcaaa taggatatct ggatacaccc cagaagaaat
     9841 accaagtttt gcatactcgt catctgtgta attggatgtt ccggtcttac cggcttgata
     9901 taacccagcg atttgcgcat tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc
     9961 tgtgatcata taagctgttt ctggagacat ggctgttttt ccatctggct cataagtttc
    10021 ttctgttccg tcttggaaga cgattttatt aacatattga ggtttgtaat aagtccctcc
    10081 attggcgaaa gccgcatagg ctgctgccat ttttaaggaa gaagctccgt attttgtccc
    10141 atcttgttct tccgtattac tagagatcgc attcgattga tggatcgtac tgtattcgat
    10201 cccaagattt tttaagaaag aagccacttt atctgaaccg acttcattga ataatttgac
    10261 tgctggaaca ttacgggata agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg
    10321 attatcccag tttcctacag gagtcgaagt cccttcatag ttatacggtg cgtcagtaat
    10381 agtttttcca gtagaatatt tcaaatattc aaaagctggt ccgtaatctg tgacaggttt
    10441 catcgtagaa ccaaaatcac gggaagtatt cactgctaag ttgttgccta aagttacgtc
    10501 ttctgcgatg tgacgtccgc caatctgggc ttttactttc ccggtattgg tgtcgataag
    10561 ggtggaggca acttgcatct cgtcgtctgg gtaagagaca tactgatccg tgttgacgat
    10621 atcatatagt ttcttttggg cgtccagatc caaattcgta taaatctcta aaccgtctgt
    10681 gtagacattc ttatctgttt tttcttgtac ttcattgatg acttctttga cataattatc
    10741 gacgatctta gtattgtcat cggattgagt tagttcttgc agaccatccg ttaccggtac
    10801 attaacagct tgatcatatt ctgtttgaga gattttttcg ttttgaagca tcgtatacaa
    10861 aacagtatct cgtcttttct tcgcctgatc tggataaaca taaggatcat aggcggaagg
    10921 ggcttgaggc atcccagcaa gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt
    10981 accaaagtac atctcagaag ctgtctccat cccgtacaag ccgtttgaca tatacacttt
    11041 atttacgtag taggtcaaga tctcttgttt cgattttttt tgttccaatc ttacagccat
    11101 ccacgcttct tgggccttgc gtttcagagt ctgatcctcg gcactggtgg agaagaagga
    11161 aagtttgatc aattgctgtg tcaaagtact tccgccttgc agtccgccag aggtaaagtt
    11221 agagagggca gaaccgatga tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg
    11281 atcttctacc gacacgatcg catcttctaa tgttttcggt aattcattcg cagaaatttt
    11341 ttcacgcttt tctgcgccta aatcttcgaa tagttcgcca tcttgtgtat atagtttgga
    11401 agatacggta gcgtctaatt ttttatccgt caattcgggc gcatcttttg cgtaatacca
    11461 gaaaagtcct acacctgcta aaaagaggat acagaaaaat gagagtattc ctaaaaggat
    11521 cttgataaat aatccttttt tatgtgttcc agaggacttt ccagaacctt tgccagaatt
    11581 cttcttcaca ctctttttag gagtaggctg tttctgtcgt ctagagcttc tagtttgttc
    11641 atttgccatt cttgctttct ccttcaatag ataatgttga taaatagaag tcaaccgcgt
    11701 ccaaataagg aatccgcgga gaaataccag ttttgatctc tattcctagt tcttcgatca
    11761 aagataaagg tagtgatttg cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat
    11821 gaacactatt caaataaaaa cagcgcttca aagacgaaaa ccataacaag acaaagcaga
    11881 ccccattttg ttcgaggcat gctttcatat gttcgatttg atgggcatgg aagtttttaa
    11941 agggaaaaga tgttttgttc ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat
    12001 agacgccatt atagtctgta gttgaggctt gggtaaagta ggcttccttg atcacagcag
    12061 cacttctctt aggatagtct actttcacga tttggatagg ggtaggcttt ttatggataa
    12121 ccgccaaccc gcgggaaaga taatatttat tactttcatt gattgcttct tcaaagcgca
    12181 tcccccgatt gccgaattca gtgatctgat ttcttttttc ttgaattttt tcgacgggag
    12241 aggcattctt ataataaggt tggccatttg gatagcgcaa taccatcttt aatcacactc
    12301 ctaggccaca ttatagcaaa aaagcggata gaaagaaaag gaatctgaga tggataagtc
    12361 aaaagtaatc tatgtgtctg ggtatcgaag tttcgaactc ggtgttttta aagaaaatga
    12421 tcctaaaatc caagttgtta aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga
    12481 agggctggaa tgggtacttg tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat
    12541 agtagcggaa ttgaaagaag aatatccgga actatctctt ggtattttgt atcctttttc
    12601 tgattttggt aataactgga atgaagggaa ccaagaaaaa aaacaattag cagaacaatt
    12661 agcagattat gtcgaagctg ttagccatca accctaccaa tcacctagtc agctaaagaa
    12721 tcacactcgt tttatacttg agcatacagt gggctgtctg ctgatctatg atgaagaatt
    12781 ccctggaaaa acgaagtatt tttttgaaga tgcacagaat taccaagcag atcatcccta
    12841 cgaaattcgg ctgatcagta tggacgatct tcaaaatttt agtgaataat cgatattgtg
    12901 tttgatttat caactttttt cctatacaat gaaaaaggta gaaatgttat tacgtaaatg
    12961 aaaactagag gtgtaaatat ggcgaatttg gtttatagtc ctaaagacat tttacaacaa
    13021 gaattcaaaa caaaaatgcg cggctatgat cccgttgaag ttgatgaatt cttagacaat
    13081 atcatcaaag attatgaaac ttacagcaaa gaactccttg ctttacaaga agaaaacgat
    13141 cgattgagtg caaaagtagc tcaattatct aaaactcaag gagcagctca aacacgcgtg
    13201 caacaaacag aggcaccaaa gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg
    13261 tcaaatcttg aacgtgaagt atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa
    13321 ccagcacaac ctaatccaaa taactacaca aatgcagaca caagcttaga tgataatgaa
    13381 aaaacacgtc aattttaatt tttgattaat gaatttgcgg ttttcgggta atcgcggtct
    13441 gacttgatca ggctgaggaa agtccatgct cgcacaggct gtgatgcctg tagtgttcgt
    13501 gcttagcgaa accataagct aaggtactct ttggagtaac ggcaggaaaa caagctaagg
    13561 cttcggctat gcttaagtat ccttgaaagt gccacagtga cgaagcggct ggggaaaccc
    13621 atccggtgga acgcggtaaa cccctcgagc gagcaaccca aacaatggta ggggcgcttt
    13681 ttgtctggaa gaagaacagg acaaaaaggc agtataactg cagatagatg gttaccgctt
    13741 tgttttttct ccctggaaaa gcaaagtaca gaacatggct tatagaaaac cgcaaacatc
    13801 agggaattag gtcgtgacaa aagtaatgcg ttataagaaa caagaaaaac atttgaaagc
    13861 tatttttcaa tacttcagat gaatcgttct tattacaaaa caaacttttg tatcaccggt
    13921 tattcggaat ttgagtaaaa gtgtggggta ggactgatgt cccacctcct ttttttataa
    13981 ataaggagat tgtgtatgga aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc
    14041 gggctagaag cactggttgg taaagaatta agagatcttg gaatcgaatg ccaagttgag
    14101 aacggacgtg cacgttttga aggaacaatg gaaacgatcg ctacagctaa tttatggtta
    14161 cgtacagcag atcgagtaaa aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa
    14221 ttgtttgaaa acgtaaaagc attgccttgg gaagattttc ttccactaga tgcagctttt
    14281 cctgtagctg gaaaatcgat caagtcgaaa ctatatagca cacctgattg ccaagcgatc
    14341 acaaaaaaag caatcgttga acgtctccgt acctattatc accgtccagc ttctgtacca
    14401 ctgacggaaa ctggcgcaca ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg
    14461 gtaactctgg atacgacagg accaagttta ttcaaacgtg gttatcgctt agaaaaaggt
    14521 ggagcaccat tgaaagaaaa catggcagca gcacttatta tgttgacaaa ttggcgaaaa
    14581 gaccgtccat tttatgatcc tgtatgtggt tcaggtacgc tttgtattga agcagcctta
    14641 ataggccata atatcgcacc aggattcaat cgtgagtttg cttgcgaatc ttgggattgg
    14701 ttcagtcaag aaatcatgga aaatgttcga tcagcagccg aagaaaaagc agattatgat
    14761 attgaattag atattactgg ttcagatatc aatggacgga tgattgaaat tgccaaagcc
    14821 aatgcagaag aaatcggatt aggctcttcg attaccttta agcaacaagc agtcaaagac
    14881 ttcaaaacgg ataaagaata cggggtcatc gtggctaatc cgccttacgg agaacgtcta
    14941 ggagaagaag aaactgttcg gaaattatat aaagaaatgg gcgatgtatt ccgtccatta
    15001 aaaacatgga gtaaatatat cttgactagc gacttagcgt ttgaggaatt ttacggacaa
    15061 aaagcaacca aaaaaagaaa attgtacaat ggtgcattgc gtacggatct attccagtat
    15121 tggggaactc gtccgcctag aaaaaaagag aaagacgaat aatcttacta attgattcat
    15181 tcaattggat gtttggagga atagggaatg aaagaaaaag agtttttaca agaagtaaaa
    15241 gaaatcaatc ttctacaaca agctttagga atcctcgatt gggatacaca aacaggtatg
    15301 cctgaaaaag ctagtgcata ccgaagtgaa gtagacagct atctttacag catttatttt
    15361 tcaaaaaaaa tcggtccaaa aatcaaagaa gccattcgct atttttctga acatccggaa
    15421 gaattgtcgg aagttggaac tgccgttttt gccaaagtaa aagaagagta tgatttggaa
    15481 catcgcgtac cggaagatct tatgagaaaa tatacagctg ccgtttcttc agcacacgga
    15541 caatggcaaa aagctagaga aacacaacaa ttttctgatt ttcaacacgc attgaatcaa
    15601 aatattgaat taacaaaaca gttgatacct tactggagaa aagatgaaaa gacgaattat
    15661 gatgttttgc tgaatcaata cgaacccggg atgactgtcg aaatccttga taaggtattt
    15721 gatcagctta aagaaggaat tctttcgatc cgccgtacgc tccaagaaaa agggactgaa
    15781 cctgaaacag actttttaaa tcggagaatg acgaaaaaac aacagaagag atttgtagtg
    15841 aaagtaatcg aacaacttgg ttatgatttt agtcgtggtc gtttagatga cactgtccac
    15901 ccatttatga ctggaattaa tccgaatgat gcacgaatca cgacacgatg gaacgaaaca
    15961 gatttcaaaa tggctgtttt tggagtgatc cacgaggctg gtcatggtat gtatgaacaa
    16021 gacatcgatg ataaatatgc gtatacaccg gtttatgaag gtacatcaat gggtatccat
    16081 gaatcacaat cgttgtttaa cgagatcatt attggaagta gtcgcagctt ctggaaaaag
    16141 caatatcctt tctttcaaga atgtgcagaa ggaacattcg atgatattcc ttttgacaaa
    16201 ttttatcgtt cattgaacca tacaaaatca agtttggttc gtatcgaggc agacagtttg
    16261 acttaccctc tccatatcat cattcgttat gaaatcgaaa aaatgatttt caatgaaggg
    16321 gtagatgttg ctgaactacc tgaaatatgg aatcaaaaat atgaagagta tctgggaata
    16381 cgaccaacaa atgatgtgga aggcatccta caagacgttc attggtcagg tgcaagcttt
    16441 ggttattttc cctcttatgc attagggtac atgtatgcgg cacaattact tcattctttg
    16501 cagcaagaaa tagatgtgga aaatgttcta gcgtcagacg attattctcc aatcaaaaac
    16561 tggatgtcag agcatatcca tcaatatggt gcatctagaa aaccaaatca attaatcatg
    16621 gatgcaacac atgaaccgct taatccgcaa tacctgatcg actatatgaa agacctgtat
    16681 tttaatgtat atcaggtaaa ataaaataca atttacaaag atagctgaaa ttcatctaat
    16741 ctatagatgt tttcagctat ttacttaaga aagaagtgga acgaatggat aaattacaag
    16801 tgatcgctga ttatagtatg gaccaattag cgcatgatca aacaggacat gggagcgatc
    16861 ataccaaaag agttgtcaag ttagctgaaa aaattttgga gacggaaccg caggcagacc
    16921 gctttgtgac gcttgcagca gcttacctac atgatacgat tgatgataaa gtcgttaaaa
    16981 atgagaacga ggcaaagcaa cagcttcgtg tattccttcg ctctttgcca atagtagagg
    17041 aacagatctc gatgattttt tccattatcg aaaatatgtc cttttctaaa aatctctctg
    17101 aagcggccga actatctctg gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat
    17161 taggagcaat cggcattttg cgtactgctt atttcggcgg tggacatggg cacccgattt
    17221 ttgatcctga actgtatccg caaacattca aggacaaaga aaattaccga aaaggcacga
    17281 cagtgatcaa tcatttttat gaaaaactgt tcttgctgtc tgataaaatg aatactgatt
    17341 acggatatga agaagccaaa cgaagagaaa ccttcatgcg tgaattctta gaagaatttt
    17401 ttctggaatg gtctattgac gatgaaaatt ttactccaga caattggatc aaaaataaca
    17461 aaaaatgatg gaggttgtga cagaagtggt cagctccaag atatttcgga tcattttccg
    17521 taaaagttac ttctggaaca ctgtttacca agaggtcgtg agcctgccgt tcagctccga
    17581 gtcacaagga acaatttcaa aaaatagctt ctcgtgtttg atgaatattg taacttgtga
    17641 ttcggaattg gcaggagaac accgtttatt cggaagaata ggactgtgac aagacttttg
    17701 tcacagtcct tttttcgaat tcaacaagta aaacttttaa gaaatattca gagagtaaaa
    17761 ttaaaaattg ctgataaacg aagatggaaa ttttatgtat ttttattggt ctatgcaata
    17821 aataaattaa atattcagaa tacaattgat tttaacgaaa gggcttctat caatggttgt
    17881 gttgtattta tctgcccagg tagagtaaaa tagatcatta tctaaaaggt aattgttcat
    17941 actatataag tcatttgaaa gtaaaaagat actgtaaata aaaaatgtgg ttaaataatt
    18001 ctcataatca gtgtaagcgt tatcaaaaat atagcatact tgctttgtag atgaatgaca
    18061 cgatatatgt ttggtatgtt ctgcaagaca gatgaatatg gaggtggaga caatgtcttc
    18121 caaagaacta acaatgtatt tagagaaaga attggatcaa ctgaaagaaa aggggttata
    18181 caatacgatc gacatactag aaagtgaaaa tggtgcttgt atcatcgtag acggaaaaaa
    18241 gatgatcaat ctagcatcca acaattatct tggatttgcc aatcgcgaag aattgaaaaa
    18301 agcatgtatt gaagcaaccg aaacttatgg tgtaggagcg ggagcagtcc gaacgatcaa
    18361 tggttcgctg aaaatccatc agcaattaga agaaaaaatc gcagaattca aagggacaga
    18421 agcagcaatc gcctttcaat ctggattcaa ctgcaatatg ggcgctatct ctgcagtaat
    18481 gacaaaagaa gatgctatct tatcggatga attaaaccat gcatctatta ttgacggttg
    18541 ccgcttatcc ggagcaaaaa tcatccgaat taaacatcaa gatatgaaag accttgagaa
    18601 aaaagcaaag gaagcgatag aaagtaagaa atataaaaaa atcatgtata tcacagatgg
    18661 tgttttctcc atggatggag acattgcacg attgccggaa attattccga ttgttgaaaa
    18721 atatggactg atcacctacg tcgatgatgc gcacggctca ggcgtaactg gaaaaggtgc
    18781 aggaaccgtc aagcattttg gattaagcga caaaatcgat atgcaaatgg gcacgttatc
    18841 gaaagctata ggtgttgttg ggggttatgt agctggatcg aaaacactaa tcgactggtt
    18901 gaaagctaga agtcggccat tcctattttc tacttcgcta actccaggtg cagctgcagc
    18961 tgcattggct tcgattactt tgatgcaaga acatccggaa cttgtcgaaa aagtttggga
    19021 aaatgctaat tattttaaag aagaattgaa aaaagtaggc tataacatcg gtatgtctga
    19081 aacacctatt actccagtca ttttgggaga cgaaaaagtg actcagactt tctctaagaa
    19141 gctgatcgaa catgggattt atgctaaacc aatcgtttat ccaactgtgc ctcttgggac
    19201 aggcaggatc agaaatatgc cgactgcgga acatacgagg gaaatgcttg atgaagcagt
    19261 agcagtttat caaaaaatag gaaaagaaat ggaaattatt taactaaatt tagttttttc
    19321 gtctatttaa aattagagaa catgttcacg catatattgt taatcatctg aagatagtag
    19381 taaaaaagat cagggaggat catcatgaaa aaagtattgg tgacaggatg tttaggacaa
    19441 attggttcag agttgatcac acggctacgt caagaactag gcagtgaaaa tgtcattgcg
    19501 acagatatac gcatgcctga agtcattgag aaaaacgata tctttgagat cctagatgta
    19561 atggattacg aaaaaatgcg cagcatagca gaacaatatc aagtggacac attgattcat
    19621 ttggctgctc ttttgtcagc tgttgcagaa gcaaaaccac aattagcctg gaatttgaac
    19681 gtgactgggc tggtcaatgc attggaagtc gctagagaat tccagctgaa attttttacg
    19741 ccaagttcaa tcggcgcgtt tggtccaaat actccaaaag atcatacccc acaagatact
    19801 cttcagcgtc ctacaaccat gtatggggta acgaaggtag ctggagaact actatgtgat
    19861 tattattatg tgaaatatgg cgtagatact cgtggtgtcc gtttccctgg attgatttct
    19921 tatgcaacct tgccaggagg cggtacgaca gactacgcag tcgatattta ttattcagca
    19981 atcaaagaag gaaagtacac tagtttcatt gatcaaggta catcaatgga tatgatgtat
    20041 atgccagatg cgatcgatgc cattataaaa ctcatgaatg cagatcctga tcgattggtt
    20101 catcgaaatg ccttcaatat atcggcgatg tcttttgacc cggaagagat caaagcatct
    20161 attcaaaaaa tcataccaga ttttgaaatg gattatgatg tggatcctgt acgtcagtca
    20221 atagcagaat cttggccaaa ttcactagat gtttcttgtg cgcaagagga atggggcttt
    20281 tcgccgaaat acgacttaga tgtgatgaca aaggatatgt tggaaaaact gaaagaaaaa
    20341 ttggtagaga cggtataaaa caagaaccac atccaaaaag ttttttatac ttttgggatt
    20401 ggctatggta tgaaagggga caagggatgg aagaatatca agccacgcca gtaaaagaag
    20461 taaaaataga aaatgaatta aaacgtacga tgggattttt tactgcttta tcgactgtta
    20521 tgggcacagt gatcggtgca ggggtatttt ttaaagccgc aagcgtagca gaagttacag
    20581 gatccgccag tttgcatatg ttttcttggt ttctcggtgg tatgatttct gtctgtgcag
    20641 gactaacagg agctgaactg gcagctgcta ttccagaaac aggagggatg attaaatata
    20701 tcgaacggat ttacggaaat acagcagctt ttttattggg ctgggcgcaa gtcgtgattt
    20761 attttccagc aaatgtagca gccttgtcga tcattttcgg tacacagttc gtcaatctat
    20821 ttggtctttc ccagtcaatg attgttcccg tagctgttac agctgctgta tcgatcctgt
    20881 tgatcaattt tttaggttct aaagctggag gggcttttca gtcgattacg cttgtctgca
    20941 agcttattcc tttgtttgtg atcgtcattt tcggtttgtt ccgacaagaa ggagtagact
    21001 tccaactgtt tccgatccaa gcaggagaaa atctttcatt tttctcagcc ttgggagcgg
    21061 gcttgctagc aacgatgttc gcatacgacg gttggataca cgtaggaaat atttcgggag
    21121 aattgaaaaa gccggcgaaa gatttgccaa aagcaatttc tttaggaatc atcggaatca
    21181 tgatcgttta tttattagta aatgcggtgt tcttaagaac cgcatctatc gacggagtcg
    21241 caggaaacag caatgcggca agtgacgttg caaaaatgat ttttggcggc ttcggtggaa
    21301 gactggtcac agttggtatc ttgatttctg tttatggaac catcaatggc tataccctta
    21361 ctggaatgcg tctcccttat gttatggcaa aagaaaacaa cctgcctttt agcaagctct
    21421 ttgctaaact tcatgataaa actaaagttc ctgtagccgc aggtatctta gagctagtca
    21481 tcgctattgg catgatgatg atcggtggtt ttgatacact aacggatatg ctgatctttg
    21541 tcatttggat tttttatacg atggtctttg ttggtgtcat tcttcttcgt aaaaaagaac
    21601 ctgatttgtt tagaccatac aaagttccaa tgtatccgtt tattccacta gtagcaatca
    21661 ttggagggac ttttatcgta tcaagcacgc ttatcactca gaccttttta gcttcgatgg
    21721 ggatagtatt gacacttgct ggtatcccga tttatctcta tttaaaacgt agatagctaa
    21781 aacattcgaa ttttattaga actgcacttc tctgccaaac aaaatatttt atttaaatga
    21841 ggagtacgtg gaagaaatgt caaaagtact cgttaatttt gatcacgtaa atgtcgtttt
    21901 gtctacgatg aaatagcaaa gtaaaagaca gaaatcgggc tcaaccgatt tctgtctttt
    21961 actttgattt gcgtaactga actattcggc gtacgccgcg ataaatattt aataaaacca
    22021 agcaaccaga aatgaaaatg ggaagccaaa tcagatagac agggtagcca agcactttag
    22081 acaaagcatg tggagtcatt ttatccattt caagaagtgc ttgctgccaa gtctgaagat
    22141 tgaagactag catgaaaccc gaaataatca ggagaataaa tcctaaataa tgaattgtag
    22201 ttctttttct tacataagcg atcgtagcat aaaggaacag taaaaaaata ggtacgccaa
    22261 aaacaaagta catattcagc atctcgacac cccctttgtg tttatcttac tttactttga
    22321 aagaaatgag aaataatagc ctcgttactt gcttgaaaca gcaaaggact gctaagataa
    22381 gagagataca aagaatcaag gaggagtcat atggcaagtt atatcgaacc gattcgtttt
    22441 gcgctgattg tttttccatt tttagcctta gcgatctcat ctgttttttt tatttatgaa
    22501 tatcgaaagt atggaacatt tctagtaaca agggcagtta ttttgtattc tttcgttttt
    22561 tatttattgt gtgcgtattt tttggtgatc ttaccacttc cggcaaagga gacggttgcc
    22621 caaatgacag ggccaaagat ggagctgcat ttatttgctt catggcaaca tttcagagac
    22681 aacacggtat tggtcttgtc tgaacctagt acctacctgc cggcaatgaa gcagagtgta
    22741 tttttggaac cagcttttaa tgttttatta ctcataccat ttggcattta tttaagatat
    22801 tatttccggc tttcttttat gaaaacgatg attgctagtt tctgcctttc tctatttttt
    22861 gaattgacgc agctaagtgg actttatttt atctacccaa gaccataccg cttatttgat
    22921 gtgaatgatt tagtgacaaa tacattgggt ggaatcattg gttttgtcgt tacgccgtta
    22981 tttacattta tgcttcctac aagaagacga atggatgaac tttcttatga aaaaggaaag
    23041 actgtctcat tgactcgacg cttggtcgct tatttgatcg actggacatt tttatctctt
    23101 attaatttgg tagcggcgat tttctttaaa attgcgttcg attcaggggc agttacttct
    23161 catatctggt ggcttttatt agaagtaggg gtatacttta tgattgtacc gtttataacc
    23221 aatggatgga cacttggcaa aaaaattgtg cgtatccgtt tggtggaaga agaccgcgaa
    23281 cgaatttctt taaaagcatt gatgatccgc tacgggtatc tgtactttct tttttatggt
    23341 ttaagctggt tggggcagtt gcttcagcaa atcatgaatt cgacaacgaa tacggtgttg
    23401 cagatgagtg cagtattcat ttctctcggt gtcttgttac tacaaggtat ttttatttta
    23461 aatatcttgc tatcattttt gagaaaaaaa cggattctgt tttatgaaaa agctagtcac
    23521 acatatactg ttagcacgat tggccaaaca aaggatgatg attctcgaaa ttagctgagc
    23581 aaaaacgctt tgtctgtgtt ataatgccaa agtcgtgata atttaaagag aagaggtgca
    23641 cgaatgaata attttgatat attccgcttc aaaccgttta tcaatgaagc gttaaccgca
    23701 aagggcttca aacagcccac agaggtccag gaacgtttga ttcctttaat tgcaaaagga
    23761 aaaaatatca ttgggcaatc tcaaactggt acaggaaaaa cacatacgtt tttgttgcct
    23821 ttgtttgaaa aaatcaatgc agaaaaagag gaagtacaag ttgttattac agctccaagt
    23881 agggaactag ctgcacagat ctatgaagca gctacacaaa tcgctaaatt ttctacaccg
    23941 gagatccgtg tctcgaattt tgtcggcggt acagacaaac agcgtcaatt aaatcgtctg
    24001 catcaccaac agccacaggt tgtagtaggc acgcctggta gaatattaga catgatgaat
    24061 gaacaagcgc tgaagattca caccgcattt gcatttgttg tcgatgaagc ggatatgaca
    24121 ttggatatgg gttttttaaa tgaagtagac caaattgcaa gtcgattacc agaaaagctg
    24181 cagtttttag ttttttctgc cactattcca gaaaaacttc gtccattctt acgaaaatac
    24241 atggaaaatc caatcattga agaaatcaaa ccaaaagcag ttatttctga agacattgaa
    24301 aattggctga tttcaacaaa aggtaaagat gagaatcaaa tgatttatca actgctgaca
    24361 attggtcatc cttatttagc aatcgtattt gccaatacaa aacagcatgt ggatgaaatt
    24421 gccgattact tgaaaaacca aggcttgaaa gtagcaaaga ttcatggaga catcacccca
    24481 agagagagaa aacgagtgat gcgacaagta cagaatctgg agtatcaata tgttgtagcg
    24541 actgatttag ctgctagagg gatcgatatc gaaggtgtct ctcatgttat taatgcagaa
    24601 attccagaag atctagactt ctttatccat cgtgtcggac gtactggacg taatggctta
    24661 aaaggaacgg ccattacgtt atattcgcca aatgatgagc aagccattga tgcgatagaa
    24721 aaattgggcg tctcatttga accaaaagaa atcaaaaacg gggaaatcgt caaaacatac
    24781 gatcgtaata gacgtgccaa acgagaaaaa tcaaaagatg tattagatcc cacgctgatt
    24841 ggtcttgtga agaagaaaaa gaaaaagatc aagcctggtt ataagaaaaa aatcgattgg
    24901 gcaattgcag aaaaaaataa aaaagaacgc aaaatcgaac gcagacagca aagccgtact
    24961 gctcgtaaaa ataagaaaaa ttcacataaa taaaaaacaa gaggttgtga caaaaggcgt
    25021 gtcacaatct cttgttttta agctagactt tttttcattc cgatacaaag tacttcatct
    25081 tcaataaaag gttctgtgca agtatgatac ccttgttttt cataaaaatc tacagccgtc
    25141 atttcagcta ctacataact agtctgacaa ccttctgaaa ccgcccgttt ttcagcagct
    25201 tcaagcaact gaccgcctaa cccttttcct cggtattctt ttgctacgca aaatcgttcc
    25261 ggcttgaagc aagtaggtgt tttcttttca taacgcaaag tagctactgg tcttttttga
    25321 tcaagaaata gataataggt ttgttgcggg atatcttttc cgtcgaattc tagttctgca
    25381 ggaatccctt gttctaatac aaaaacttga tatctcaaaa agcagctagc tgcgcgtatc
    25441 cattcatccg ctccaactat ttttttcatc atattcctcc ttgtcatgaa tagcagagat
    25501 gtattgattc ctttattctt atcgtaccaa aaatcgtgga gtatgaccaa aaggaaatga
    25561 aaataaaaat tgagatattg acagtgatca atagaatgcg tatactaatt aagtacgaaa
    25621 aggatatgat ttgtttttta agactcacag aaaattcttt cttggctgaa aaaagaatag
    25681 tcactaatca aattgctccc tttttctatt ttggtagaaa taccgacgca gctcagcgtt
    25741 atcgagttta agaggtaatg attacacatc attgcaatca aggtggtacc gcgagttttc
    25801 gtccttggct gtgatggtgt ttttttgtat ttaatttata gaaagcaggt aatgacatga
    25861 aacaattaag tagtgcacaa gttcgacaaa tgtttcttga tttcttccaa tctaaaggac
    25921 atacgattga acctagtgcg tctttgatcc ccgtgaacga tccaacctta ttgtggatca
    25981 actctggtgt tgcaacattg aaaaaatatt ttgatggttc agttgtgcca gaaaatccaa
    26041 gaatcacaaa tgcccaaaaa tctattcgaa caaatgatat cgaaaatgtc ggtaaaacag
    26101 cacggcacca tacgatgttt gagatgctgg gtaatttttc gattggtgac tatttcaaaa
    26161 atgaagccat ccactgggca tgggaatttt tgactgatcc aaaatggatg gcattcgatc
    26221 cagaaaaact ttatgtaaca gtttatccaa aagatacaga agcaaaacgc atctggcatg
    26281 aagaagttgg cctgccacta gatcacatta ttgatgtaga agacaacttc tgggatatcg
    26341 gtgcaggccc atgtggtccg gatacggaaa ttttctatga ccgtggagaa gcttataacg
    26401 atttagcaga agacgatcct gaaaactatc caggaggaga aaatgaacgt tatttagaaa
    26461 tctggaactt agtattttca gaattcaacc ataagcctga taacacattt gaaccacttc
    26521 ctcataaaaa cattgatacg gggatgggat tggaacgcat ggtctcaatc gttcaagatg
    26581 cgtcaacgaa ctttgagaca gacttgttta tgcctattat ccacgccgta gaaaagctaa
    26641 gcaaacaagt aaaatatggt gaaaacccag taacagatat ttcttacaaa gtaatcgcag
    26701 atcatattcg cgccctttca tttgctattg gcgacggtgc attgccatcg aatgaaggga
    26761 gagggtatgt cttacgccgt ttgcttcgtc gtgctgtcat gcatgggaaa aaattaggta
    26821 ttgaacaagc attcttatat cgtttagtcc ctgttgttgg ggaaatcatg gtaagttatt
    26881 atcctgaagt acttcaacaa aaagaattta ttgaaaaagt tatccgtaca gaagaagaac
    26941 gtttcaatga aacaatcaat gaaggtttgg aaatcttaaa tcaagtgatc caagaagtaa
    27001 aaaacgaaaa cgggacgact ctagcaggaa aagacatctt caaactatac gacacatatg
    27061 gcttcccggt agaattaacg gaagaggtgg cagaagatga aggactgaaa gttgatcatg
    27121 ctggttttga aaaagaaatg gatgcacaac gcgaacgtgc tcgctctgcc cgcagtactg
    27181 aaaaatcaat gggggtacaa tctgctttat tgacagatat caaagtagaa agtaaattca
    27241 ttggatacac agaacaagaa tcagatagtc aattattggt catcgttaaa ggtgagtctc
    27301 ttttgaatga agtcgcagaa ggtgaagctc aaatgatctt tgatcaaaca cctttctatg
    27361 cagaaatggg tgggcaagta gcggatcaag gatggatcat gaatgaagta ggcgaaatcg
    27421 tagcagttgt tacagatgtt caaaaagcgc ctaatggtca atttatgcac accgtagaag
    27481 taaaagcacc aatgagtgaa ggacaaacct atcatctatt gatcgatcaa tcgatgcgca
    27541 atcggatcat caaaaaccat acagcaaccc atctactgca caaggcattg aaagaagtac
    27601 ttggcgagca tgcaaatcaa gctggttctt tagtcgctcc tggacattta cgattcgact
    27661 ttacccactt tggtcaaatc acttcagatg aattagctga aatggaaaga atcgtcaacg
    27721 aaaaaatctg ggaagctatc ccagttgaaa caattgaaac agatatcgat acagccaaaa
    27781 acatgggtgc aatggctttg tttggtgaaa aatacggaca tgacgttcga gtagtaaata
    27841 ttggcggctg gtcaatcgaa ctgtgcggag gcacacatgt agcaaataca gaaaacatcg
    27901 gaatcttcaa aatcgtttct gaatcaggaa ttggtgcagg cgtgcgacga atcgaggctg
    27961 taacaagtaa agaagcgtac gaactacttg aagaagaaga aaaacaacta aaacaagtag
    28021 caacaattgt taaatcacct caattgaaag aagttgtttc aaaagcggaa caattgcaac
    28081 agcaagtaag agagctacaa aaggaaaatg aacaattagc tagcaagtta gcgaatcaac
    28141 aagctggtga tgtattcaaa gatccagaaa cgatcaatgg aaaaacaatc attgcagcac
    28201 aagtaaaagt gaaagatatg aatcagctgc gtcaattagc tgaccaatgg aaacaaaaaa
    28261 atatttcaga tattcttgtt ttagcaaacg aacaagaagg aaaagtcagt ctgctagcgg
    28321 caatgacgaa agaatcaaat gaagctggac taaaagctgg cgacttgatt aaagcaatcg
    28381 caccgaaagt aggcggtggc ggtggtggac gtccagacat ggcacaagct ggcgggaaaa
    28441 atcctgctgg tattccagat gcacttaaag ctgcgaaaga atgggttgcg caacaatagg
    28501 gcgtttacgt ttgacagaaa aacaaaagtt caaacattga acgaaacatg attatggggg
    28561 cgaagacaaa caagtcttgc cctcatactt ttttcaattg gatggcgcat ctaatcaaat
    28621 ggtcggattc ggatggtctt tagttgacga aagtgacaga aatttttacg attagtataa
    28681 ataaaaaggg gtgaaaaaat gaatagtaga aataagacct ttcgactagt cttacgtgcg
    28741 atcttgttag caatcatcat cgtacaagcg atggttcctt ggctggggtt tattccatta
    28801 ggattcatta gtttaacaat tatccatatc acagtaatta ttgctgcagt agtattaggc
    28861 ccaaaagatg gcatggtgat tggcttgttt tggggaatcg ctacaattgt tcgtgcctat
    28921 gccatgccaa caacgccatt tgatacatta gtgttcacga atccgattat ctcggtagtt
    28981 cccagagtac ttgttggctt agtagcaggc ttggttttcc actggattta tcaaaaaaat
    29041 aaatcaataa cgatcagctc agcctttgca ggggtactgg gttcattggt caatacagtc
    29101 ttagtgttag gattcatggg attattttat acaggtgcaa cggcagatgc ctatggtgta
    29161 gactctgctc ttttatttaa aaccttagca ggggttgcag caatcaatgg gataccagaa
    29221 gcaataggtg cgggaatcat aacaccatta ctagcaaaag cgttatttgc agcaacaccg
    29281 ttgaaaccag aataatgacc atcttttaat agtaatagga cgcacgatag atagaaaaag
    29341 gaagagagga gaggaaaaca atcgtcctct ccatcttttt acctgagcaa caagcaaact
    29401 agaaggggta aaaaacttgg ctaagaaaca agaagaaaaa aagaaacaaa taccgcaaca
    29461 gcggacaaaa aaaggagtac tagcgggtat cttaggattg gttacgaatc tgatcttgtt
    29521 tgtctctaag ctaatgatcg ggcttttttc aggaagtgtc tccattatgg ctgatgcagt
    29581 gaacagtcta tcagatactg cttcatccat attgactttg atcggattta aaattgctgc
    29641 aaagccagca gatcaggaac atccttatgg acacgaacgc ttcgaatata tcagtgggtt
    29701 atttgtttcg atcatcatta cttatgtagg atttcaattt ttagagtctt ctgctgaaaa
    29761 aatcatccat ccagaaaatg tctctttaac acccatcgtc ttttttgtcc taatcatttc
    29821 catctttttg aaatttgcac aaggcaaaat gtatcaggtt attgcgcaat ccattgattc
    29881 tgaaacattg agagctacag caacagatag ttataatgat gtctttacta cactggctgt
    29941 attgctttca gcagcaatcg aatgggtaac tggatggcgt attgatggat atataggttt
    30001 tgctttagca ttatatatcc tttatagtgg attgacgatg cttcgaaatt ttgtatacga
    30061 actattagga agtcgcccaa cgccagcaga gattgaagaa atggaagagc atttaaatgc
    30121 ttgcgatgat atacttggct atcatgattt attggtgcat aactatggac ccaataaacg
    30181 ctttgcatca gtccacatcg aagtagatga atcaatgaat ttgaatgtgg ctcataagat
    30241 tattgatctg atagaaaaag actttaaaaa gtctttggat gtggaacttg tctgtcactt
    30301 ggatccaatg cctgttgata acgaaaaata ctataatatc ttgcggcagt taaagcaaat
    30361 tcttcttgaa acaggtacag ggcttaatat tcatgatttt agagtaattc atcaaggaaa
    30421 aacgctgcaa tttgatgtag tggtccctga gaaattccag tttgttgata cagaactaga
    30481 aagaatctta cgtagtcaaa tagaagaaaa aatcggagat taccaattgg aaattacgtt
    30541 tgatcacaat tatcttttat attaaatgag aaactataaa aagactcctt aaagttttta
    30601 atttaaagag tctttttttc ttttaattgt ttatggagag cctgacgtgc aagattatct
    30661 gctcctttat ttttgttttc gggcaaccat tgcaggatca ataaagaaaa ttcggataga
    30721 agttcttgga ttctttgaag gtagggaagg aagagctcgt tcttcgttgc gtttttgtcg
    30781 accgtagaaa ctagtgtttt gctgtctgaa aaacagaaaa tcgtttcatt ctgtaagtcg
    30841 tttttcttta gaaattctaa agtttttaaa aagacttcga actctgcttg atggtttgtc
    30901 cctatagaaa gcgggataga tagctgttct tgtttacctt gatagacaat caagattcca
    30961 cctccactag gaccagggtt gccttttgta gaggcatcaa tgtatacttt taacataaaa
    31021 agagattctc ctttttgttc aaactaatgt tatggtaaga ttatatcatt actgaatcgg
    31081 tgagaggtta gagaatgaaa aaaaatattc gatggcagcc tgaactggca catacgatca
    31141 tctattggtc attgaccttg atcattctgt tttttagtct gattctttct ttagaaaaca
    31201 caggtcctta ttggaaaagc aattttgttt taggtatttt tcttgttttt ctatttctag
    31261 gtttgcggcg ttcttttatt ttaaaatcaa actctgtgat tatacgttat gctgcttttt
    31321 ggaaagaccg agaaatatct gtcggacaga ttcgatcttt gacgccaact aatactgggg
    31381 tgcaaatgat gttgcaagat gagaaagccc catctgtttt catgatgcga aaaaaagatc
    31441 aagtagtcct ttttgatcat tttgaaaaaa acggacccaa atcagaatca attccttatc
    31501 cttatacgaa aaaaagaaaa agctaatttt aaaaatcaga acctaaaaaa acgaattttt
    31561 acaggaaaag aaaagagtga gacgtacggt tcatcccgtt tagcgtctca ctctatttgt
    31621 ttatctattc atgcatacta gtatcggtag tctgaacgtt gcctttcttg acatgggcag
    31681 cttgtgttcc tcgattccct tcgatgatct caaattcgac cacttgattt tcttccaaag
    31741 ttttgaatcc ctcttcttca atcgcagtaa aatgtacaaa aatttcttct gtgtcatcat
    31801 aactgataaa gccgtatcct tttttgttgt caaaccattt tacaattcct gtggtcaaag
    31861 cactcacgac ctttctaaag tctgatcata gccttcatta taaagagtat ttataagtac
    31921 gtcaaataaa ttaaaaacct gtaattttat gttttttttc tgaataaaaa aaggtataat
    31981 tggcatatct taacaaagaa gatattttgt tgaataaagt tcgtgaaaag gggtaatcaa
    32041 atgaagaatg atctgcaaat ttcacaagaa acagagctag aaccgattgt tgaagtggct
    32101 agtaaattag atcttaagga agacgatttg gaactgtatg ggaaatataa ggcgaaaatc
    32161 aatttttctg gaatcaatcg atcaaaggat aacgctgagg ggcatttgat cttagttact
    32221 tccatcaatc caacaccagc tggcgaagga aagtcaacga tcactgtagg gttaggagac
    32281 gcgctcaata aaatcaataa aaaatctgtt attgcattgc gagaaccctc acttggtcct
    32341 gtgatgggaa tcaaaggtgg tgctgcaggc ggaggatatg cacaggtatt gcctatggaa
    32401 gatatcaact tgcattttac tggtgacatg catgctatca caacagctaa taatgcatta
    32461 tctgctcttt tagataatca tattcatcaa ggaaatgaat tgaacatcga tgcccgccga
    32521 gtgatttgga aacgggtagt cgatctcaat gatcgtgaac tgcgtaaagt aattgttggc
    32581 ttgggtgggc caatccaagg tgttccaaga gaagatgggt ttgatatcac agttgccagc
    32641 gaaatcatgg ctatcttgtg tcttgcggca gaccttgaag atttaaaggc tcgtcttgca
    32701 aggatcgttg tcggctatac atttgaccgt cagcctgtga ctgctggaga cttgaaagca
    32761 gaaggcgctc tggcattact gctgaaagat gcaatcaagc cgaatcttgt acaaacaatt
    32821 tacggaacac cagcttttgt ccacggggga ccgtttgcca atatcgctca tggctgcaat
    32881 agtattttag ctaccaagac agcattgaga ttagcagatt acgttgtcac agaggcagga
    32941 tttggtgcag atcttggcgg agagaaattt ttagatatca aagtacctaa cttgaaaaaa
    33001 gcgccggatg ctgtggtgat tgtcgccact gtccgtgcat tgaaaatgca tggcggaatg
    33061 aaaaaagatg aacttaaaaa tgagaatctt gatgctttga aagtaggttt tgcaaattta
    33121 aagcgccata tacgtaatat ggaacaatat cagctacctg tgatcgtcgc aatcaatgaa
    33181 ttcgtaacag atacagacaa tgaactcaca ctgttggagc acttgtgtga aaatcagggg
    33241 attcttgcca agagagcgag tgtatgggca gatggagcag aaggcggcgt tgatttagca
    33301 aaagctgtag tacgattgat tgatagaaaa gaagctaatt acaagccatt ataccgccta
    33361 gaagaaacga tacaagaaaa gacagaaaca atcgtcaaga aaatttacgg aggaaatggt
    33421 gttgttttca gcaaaaaagc gcaaaaacaa atagaagagt ttacgaagaa tggatgggac
    33481 aaactgccga tttgtatggc aaaaacacag tattcatttt ctgatgatcc ttcactgcta
    33541 ggtgcaccgg aagatttcac catcacgatc cgcgaaatca tcccaaaatt aggggcagga
    33601 tttttagtag ccttgactgg agatgttatg acgatgcctg gtttgcctaa aaaacctgct
    33661 gcattgaata tggacgtgac aaatgacgga gaagtgttgg gattgtttta agcttgatta
    33721 taatctacca tcgaaggagc tgtattttga ctacagcttc tttttttaga cgatagggaa
    33781 ttaattttgt ttacactagg atcattcttt ttctttattg tattttgagc tatactaaag
    33841 cataatagga gggtatgatg aaaacgacat ttataagaaa aaccggtttt tacggtatgg
    33901 gtagctcgat tcagctaaga atcaacaatg aaaaacgcag tgttctcagt catgaacaaa
    33961 agataacgct tgatctggaa gtgcccttta cgatgcaggt caccttttat tggctaaaaa
    34021 gccccgttta tacagtgaaa gaagcaaagc ctgcttatgt gatcacaatg aattacttga
    34081 ttcttcaaat ctatccgttt ttatttctag caactgggtt gggggccgtt ttcctacaaa
    34141 atttattttt cagttttttt gcagttattg tgttgattgc gttctttttc tatataaaaa
    34201 accgggcata tttgatcaag gaggaaaatt atggcaaact ctgatctttt tttaatcagt
    34261 ttcaatcgaa tcgaaaaatg gatgagagac gagatgggaa atgcacgaaa tatgggattt
    34321 accgaacttg tcagacgcct agctcaaaga aaacaattaa tgatcagaaa atacgaagat
    34381 gatcttttac agcttgctca gttaaggaat gcgatcgtcc atgatcggat cgcagtcgat
    34441 tttgtcattg cagagccaaa tgattggaca gtcaagcgaa tccagctgat cgaacaagaa
    34501 ctgattcagc ctgaaaccgt gctgcctcga tttgcaaaac gtgtaacagg ttttgaacaa
    34561 gatcttccgt tacttgaact gttaaagata gtagcagaaa aaaggtattc acaatttccg
    34621 ttatacaata aaggaaaatt tgtagcattg attactttac gtaatatcgg gttttggcta
    34681 gcaaaagaaa gccaaaaagg tccggttgat ttaacaaata aacgagcgtt ggatttgatc
    34741 atccaaaacg gaaagtacac aaattatcat tttgttccag ctaaaacaca tatttacgag
    34801 gttgaagcga tgtttcgtga gcaagggact ttggaagccg tacttatcac taaagatggg
    34861 aatcccgatg gtaatctgct cgggatcgta cgacctcgtg atatttataa acaaatagaa
    34921 aaggattgat tttttgttaa ttatttattg gatattcagc gctttgctag taggagtgga
    34981 tcagttggtc aaatggtgga tcactgataa tctagcgctt ggagagacga aaaatcttat
    35041 accaggtatt ttgtcactga atcatattcg aaacactgga gccgcatgga gtatgctaga
    35101 agggaagatg tggttcttta ctatagtgac actcattgca gtcgttgtga ttctcacatt
    35161 gatgattaaa aatcgtgaaa aaggtaatcg ctggttcatg attggattga gtttgattct
    35221 agctggtgcg attggcaatt ttatcgaccg tattcgtctt ggctatgtag tagatatgtt
    35281 ccagacagat tttatgagct ttccgatttt taacgtagca gatgtgacac ttgtatgtgg
    35341 agtgatttgt atgttaattt atattatttt agatgaaaag gatcaaagaa aaaaatgaca
    35401 gaacttaaaa cgacgattca ccatgaatct ggtaggatcg ataaagtatt gactaattta
    35461 ttcaccgatt acagtcgttc gcaaattcag ctgtggctga aaaatggagc ggtttctgtc
    35521 aatggacagc cagtcaaagc aaattacaaa gtaaaacaag gagacgagat tactgtcttg
    35581 atccccgaac cagaaacatt agatgtggtc ccagaaaata tcccgttgac gatcgtttac
    35641 gaagatgaag atgtagcggt gataaacaaa cctcaaggaa tggtcgttca tccttcagca
    35701 ggtcatacgc atggcacaat ggtcaatgcg ctgatgtacc atatcaagga tctttctacg
    35761 atcaacggcg ttattcgtcc aggtattgtt caccgaatcg acaaagatac ttccggactg
    35821 ctaatggttg ccaaaaatga ccaggcacac gaatcattag ctcagcaatt aaaagataag
    35881 acctctttgc gcaaatatgt tgctttagtc catggagaga ttcctcacga aaaaggaacc
    35941 atcaatgcgc caatcggccg ttcgaaggaa aaccggaaaa tgcaggcgat aagagaagat
    36001 gggaagtcgg ctgtcacaca ttttactgta ttggaacgtt tcgaaggatt tactctggtt
    36061 gaattaaaac tagaaaccgg aagaacacac cagatccgtg tgcatatgaa gtatatcggt
    36121 tatccgttag ctggagatcc agtatacgga ccgaagaaaa ctttgaaagg caatggtcag
    36181 tttctccatg cgcaaatgtt aggttttacg cacccgacta caaacgaaaa gatgatcttt
    36241 gaagcacctc ttccagaagt ttttgaaaaa actttagaaa aattaagaaa aaacattgca
    36301 ttttaattta gataacggta tagtaattaa caacgaaagc ataaaactta atagaattcc
    36361 tttaagttta gtcccgtgag gctaagaagg gtgggagcaa ggatacgtat ggtgcgccat
    36421 aagtagattg ctcattgaca tagtatagct atcagtgtat cagtatacgc tcagtctaaa
    36481 cattcctcct gtcctcttag ggcaggagtt tttttatgca aaaaagtagg gggaatgaaa
    36541 atgcaagcaa aagaagtagt agatcaggta accatgaagc gagcattgac tcgaattact
    36601 tatgagatta tcgagagaaa ccatagtatc caagatatcg tattggtagg tatcaaaaca
    36661 cgaggaatct acattgcatc aagaattgcc gaacgcctga agcagctaga agatatcgat
    36721 attcctgtag gagaactgga tatcacttta taccgagatg acaaaaaaga aaatccagaa
    36781 gaaccggaac ttcattcttc tgacatccca gtttccttag aaggaaaaga agttattttg
    36841 attgatgatg ttttgtatac aggacgaacg attcgagcag caatggacgc agtaatggat
    36901 tttggcagac caagaaaaat ttcattagca gtcctggtcg atcgtgggca tcgagaattg
    36961 ccaatccgtg cagattatgt tgggaagaac attccaacat ctcgggcgga agagatttta
    37021 gtagagatgc aagagcttga tggacaagat cgcatcatga ttttgaaaga ggaagattag
    37081 aggagagaga aacatgtcgg agaaaaaagg atttcataat gatgacgtgg tgttagatat
    37141 ccacgatcgc ccaaccccag gacattgggt aggattaagt ttacaacact tgttcacgat
    37201 gttcggagca accgtgttag tccccatctt agtaggtatc gatccaggta ttgcgctagt
    37261 cagttcagga ctaggtacat tggtgtactt atttactact aaaggaaaga tacctgcgta
    37321 cttaggaagc agcttcgctt tcattgcagc gatgcagctt ttgatgaaaa cagatggtta
    37381 tccagcgatt gcacaaggag cagtcacaac tggattagtc tatttgattg ttgcgctgat
    37441 catcaaaaag atcggttcag catggctgga taaaatcttg cctccaatcg tcgtcggtcc
    37501 agtcatcatg gttatcgggt tgggacttgc ggcaaatgca gcaacaaatg ctatgtataa
    37561 tcagggcacg tatgatttca agtatattgc tgtagcgctg atcacattag gattaacaat
    37621 tttcttcaac atgtggctaa gaggttttct cggattgatt ccgattttac taggtattat
    37681 tagcggatac atcgtagcct tgctgtttgg aatcgtggat actaagccaa tcgtagacgc
    37741 tgcctggttt gctgttccta actttgagat tccctttgta caatatgaac caaagctata
    37801 tcttggagca atcacaacaa tggcacccat cgcttttgtg acgatgacag agcatatcgg
    37861 ccatcttatg gtattgaata aactaactaa acgtaatttc tttgaagaac cgggtcttca
    37921 taagacgttg acaggtgatg gattagcaca aatcgtggca gcctttgtcg gaggtcctcc
    37981 tgtaacaagt tacggtgaaa acattggtgt tctggctatc acacgtgtcc atagcgtgtt
    38041 tgtgatcggt ggagctgcag tatttgcagt cattttagga ttcgtaggaa aattaagcgc
    38101 tttgatttta agtatccctg gaccagtcat ctcaggaatc agttttatct tatttggagt
    38161 aattgccgca agtggaatga agattttaat cgagaacaaa atcgattttg ataaaaagaa
    38221 aaatctgttg attgcttcag ttatcttagt agtaggaatt ggcggactcg tctttgaagt
    38281 aggcacattt acattgtcgg caatggcatt agcaactgta cttggcatca ttttaaatct
    38341 tatcttacca gaaacttcac gaagcgaaga aaactaagga ggaaacatta tgatcatcaa
    38401 atcagaacgt atcagcttaa agcatttatt gaccgttgag gcacttactg atcaagaagt
    38461 tatgggcttg atccgcagag ggcaggaatt caagcaaggt gctaattgga ctccgcaaaa
    38521 gcgccagtac tttgctacaa acctcttttt cgaaaatagt actcgcacac ataaaagctt
    38581 cgatgtagcg gagaaaaaac tagggctaga agtgattgaa tttgaagcaa gcacaagctc
    38641 tgtccaaaaa ggggaaacac tatacgatac agtactgacg atgtcagcat taggcgtaga
    38701 cgtagcggtt atccgtcatg gtgatgagaa ctattatgac gaactgatac aaagtaaaac
    38761 aatccagtgt tcgattatca atggaggaga cggaagcgga cagcatccaa cccaatgctt
    38821 gcttgatttg atgaccattt atgaagaatt tggtcatttt gaaggtctga aagttgccat
    38881 cgtaggtgat atcactcact ctcgcgtagc taaatccaat atgcagatgt taaaacgtct
    38941 aggcgcacaa gtattcttct caggtcctag agaatggtac gacgatgaat tcgaagtgta
    39001 cggacattat atgccattgg atgaactact agatcaagtt gatgtcatga tgatgcttcg
    39061 ggtgcaacac gaacgccatg atggaaaaga aagtttctca aaagaaggct accatcagga
    39121 acatgggtta acagttgagc gtgcgaagaa gttgcaaaaa catgcaatca ttatgcaccc
    39181 agcaccagtt aaccgtgatg tagaattagc tgattcatta gtggaaggat tacagtcgcg
    39241 gatcgttcag caaatgagca atggagtatt cgtcagaatg gcaatcttag aagcagtgct
    39301 aaatggaaaa gcgtaattat aggaggatga aaaaatgaaa acactcatta aaaacggtca
    39361 gatcaataca cgaaaaaata tgacgacacc agcagaaatt tggatagaag atgggcgaat
    39421 caaagcaatc ggtacaggat tctcagaagc cgaatttgat gaagtgtttg atgcaaaggg
    39481 acaattgatc acacctggtt tagtggacgt ccatgttcat ttgagagagc caggatttac
    39541 atacaaggag acgatcgaag ctgggactcg ctcagctgct cgtggcggtt tcactactgt
    39601 atgcgcgatg ccgaatttaa atcctgtacc tgatacggca gaaaagctga gacaagtata
    39661 tgacatcatt agaaaagatg cagttgtgaa ggtattgcag tatgcgccaa tcacagaaaa
    39721 tctgcgaagt gagaaattag ttgatcaaga agcactgatt gaagaaggtg cttttgcttt
    39781 cacaaatgac ggtgtaggtg tccaaacagc tgggacgatg tatctggcta tgaaagaagc
    39841 tgcaaaaaac aataaagccc tagtagccca tacagaagat gaatctttat tatttggcgg
    39901 cgttatgcac gctggcaaga aggcagaaga acttggtctt cccgggatct taagcgtaac
    39961 cgagtcttct caaattgcta gagatctgtt gctagctgaa gcaactggtg ttcattacca
    40021 tgtttgccat gtttctacta aagaaagtgt ccgtgtaatc agagacgcaa aaaaagcagg
    40081 aatccacgta acagcagaag tttcacccca tcatcttatt ttgatcgacg aagatattcc
    40141 cgaagatttc ggcttttgga aaatgaaccc gccgctaaga ggacgggagg acagagaagc
    40201 tttgatcgaa ggcttgcttg acggtacgat cgattgcatc gctacagacc atgcccccca
    40261 tggattagaa gaaaaaagcc aaagtttcat gaaatcacca tttggtatcg taggaagcga
    40321 aacagcgttt caactgatct atactcattt tgtagaaact ggtcgattca cgttggaaca
    40381 agtgatcaat tggttggcag ttaaaccggc tgagattttt ggtttgaatg caggaacgct
    40441 aacggttggt gcccccgctg atgtagcagt ttttgatatt acacaaactt gcacgatcga
    40501 taaagaagac tttttatcaa aaggggagaa tacacctttt atcggctgga aagtaaaagg
    40561 cgaaacacaa atgacttttg tcaacggaaa acttgtgtgg cagaagggag aataaaagta
    40621 tggaacgttt gcttatatta gaagatggaa cagttttcga aggaaaggct tttggtgcag
    40681 atgctagtgt cttcggagaa atcgttttta caacaagtat gactggttat caagaaacta
    40741 tcactgatca aagttttaac ggtcaaatca ttacatttac ctatcctatg gtgggtaatt
    40801 atggggtaaa tagagatgat tatgaatcga tttctccaac atgcaaagga gtagtagtaa
    40861 aagaacatgc acgtgtagct tcaaactggc gaaaccaaat gacgctagat tcatttttga
    40921 aacaaaaagg aattcctgga atatcaggta tcgatacgcg cgcccttact cgcaaaatcc
    40981 gtgaagtcgg aacgatgaaa gctagtatag ttgatgctgg agattccttc gaacatgcat
    41041 ttgatcagct gaaagcaacc gttctagcaa ccaatcaagt gcagcaagtc tcaactaaca
    41101 aaccttatcc aagtccagga acaggtcgga aagtcattgt cattgacttc ggcttgaaac
    41161 atagtatttt acgagaattg agtaaaagaa actgtaacct cacagtactt ccctataaca
    41221 cagatgccga gacgatttta gcgctatcac cagacggagt catgctgacg aatggacctg
    41281 gtgatccaaa agatgtgcca gaagcaatcg aaatgatcaa aaaaatccaa ggaaaagtac
    41341 cgatttttgg tatttgtctc ggtcaccagt tatttgcttt agccaacggt gcagatacct
    41401 ataaaatgaa attcggacat cgaggattaa atcacccggt acgagaagta gcgaccggaa
    41461 gaattgattt tacttctcaa aatcatggct atgcagtcga tgaagccacg attgatccag
    41521 aaaaactcat cgtaactcat gtagaagtaa atgacggaac agtagaagga gtcaaacaca
    41581 gagattatcc cgctttcagt gtgcagtttc atccagatgc tgcccctggt ccgcatgatg
    41641 cactacattt atttgatgaa tttatggaaa tgatggacgc aggaaaggag cagtaaacag
    41701 atgccgaaac gtacagatat caagaaaatt atggtgattg gatctggtcc gatcatcatt
    41761 ggtcaagccg cagaatttga ctacgcagga acacaagcct gccttgcctt aaaagaagaa
    41821 ggatatgaag tggttttagt aaattctaat cctgcgacga tcatgacgga taaagaagtg
    41881 gcagatcaag tatatatcga accgatcaca cttgaatttg tatcaaggat tcttcgaaaa
    41941 gagcgtccag atgctctcct tccaacttta ggcggacaga caggcctcaa tatggcaatg
    42001 gagctggcag catcaggtat tttagaagaa ttgaacatag aacttctagg aacaaagctt
    42061 tctgcgatcg atcaagcaga agatcgtgat ctattcaaac agttgatgaa agaattgaat
    42121 cagccaatcc ccgagtctga aatcgtcaat acagtagaag aagctgtaga ttttgcaaat
    42181 aaaatcggct atcctgtcat cgttcgtcca gcctttactt taggcggaac tggtggaggt
    42241 atgtgtgaaa atgaagaaga attacgtatc gtatcagaaa atggattaag tctttccccc
    42301 gctacccaat gtttgattga acgaagtatt gcaggtttta aagaaattga atatgaagtg
    42361 atgagagatt cggctgataa tgcaatcgtt gtctgcaata tggaaaattt tgacccggtg
    42421 ggtatccata caggtgattc tatcgtattt gcccctagtc aaacattgtc tgaccatgaa
    42481 tatcaaatgc ttcgtgatgc ttctctttcg attattcgag cacttaagat cgagggaggc
    42541 tgtaatgtcc aactcgctct tgatccacat agttttaatt attatgtgat tgaagtcaat
    42601 ccgcgtgttt ctcgttcatc tgcgttagca agtaaggcga ctggctatcc gatcgctaag
    42661 ctagctgcta aaatagcgct tggattaaca ttagacgaaa tgaaaaatcc tgttacagga
    42721 acgacttttg cagaatttga acctgcatta gattatgttg tagctaaaat tcctcgctgg
    42781 ccgtttgata agtttgaaaa cggagaacgg cttttaggca cgcagatgaa agcaactggt
    42841 gaagtgatgg ctatcggtcg gaatatcgaa gagtctttat tgaaagctgt gcgttcttta
    42901 gaaatcggta cacaccatat cgaacttcct gagttggcag acatcacgga cgatgaccta
    42961 atccaaaaag tggttcgagc acaagatgat cgcctgtttt atgtagctga agctattaga
    43021 agagggtaca ccatagaaga attagcggat ttgacaaaaa tcgatttatt ctttttagat
    43081 aaactgttgc atatcgtaga aatcgaacag cagttgaaac agcagccgtt ggatgacaag
    43141 atattgaaaa cagcgaaaca aaacggtttt acagacaaaa aagtggcgga gctgtgggaa
    43201 ataaacgaag aagccgttcg ccagatgaga aaagaaaatg atttgattcc tgtatataag
    43261 atggttgaca cttgtgcagc ggaattcgaa tcaaaaacac cttattttta tagttcctat
    43321 gaatatgaaa atgaaagttc acgatcagaa aagccctcaa ttctagtact gggatctggt
    43381 cctatccgta tcggacaagg ggttgaattt gactatgcga ctgtgcattc tgtcaaagca
    43441 atccagcagg caggatacga agcaatcatc atgaacagca atcctgaaac agtttcgact
    43501 gacttttcta tttcggataa attatatttt gagccattga ccttcgaaga tgtgatgaat
    43561 gtgatcgagc tagaacagcc gataggagta atcgttcaat ttggtggtca gacagcaatc
    43621 aacttagccg aacctttgac tgctgctggt gtaaaaatct tagggacaac gatcgaagac
    43681 ttggatcgtg cggaaaatcg tgatttattc gagcaagcgc tgaaagagtt ggatattcct
    43741 caacctttag gggatactgc tacaagtaaa gaagaagccg tggttatcgc ttcgagaatt
    43801 ggttatccag ttcttgttcg tccaagctat gtattaggcg gacgtgcgat ggaaatcgtt
    43861 gaaaaccagc gagatttaga agattacatg gaacatgctg tgaaagcatc tccagaacat
    43921 ccagtactag tggaccgtta cttgcttggt ggtgaatgcg aagtggatgc gatttgtgac
    43981 ggtgaaacag tactcatccc agggatcatg gagcatattg agcgtgctgg tgtccattca
    44041 ggggattcaa tggctgtgta tccacctcaa acattatctg aatcgatcaa gaaaacgatc
    44101 gaagattaca cgatccgctt agcattaggt ttgaattgtg tcgggatgat gaatatccag
    44161 tttgtcatcc atgaaaatga ggtttatgtg atcgaagtca atcctcgagc aagtcggact
    44221 gttccattct taagtaaggt gacaggaatt ccaatggcac aaattgcgac gaaagctatt
    44281 ttgggagaga aactaaagac acttggttat gaaaacggac tttatccaga aacaaaagaa
    44341 gttcatgtta aagcacctgt cttttctttt acgaaattgc agaaagttga tacttactta
    44401 gggcctgaaa tgaaatcgac tggagaagta atgggatctg acagacaatt agacaaagct
    44461 ttgtacaagg cattcgaagc ttctggatta cacttgcctg aatatggagc cgtattattt
    44521 acgattgcgg acgaaacaaa agaagaagca ctagaattag ctaaacgttt tacagaaatt
    44581 ggctatagtc tgataggaac gaaacataca gctgcttatt tcgaaaaaaa tggtctggtc
    44641 gttgaaacag tagcgaaaat ttctgaggaa gcagcagaaa aaaatgtagt cgacttaata
    44701 agagatggta aaacacaagt ggttgtcaat acgattgata aagatcgggg aaatgcttct
    44761 aaagacggtt tcatcatccg aagagaagca gttgaacatg gtattccatt gtttacttct
    44821 ttagacacag cagatgcgat cgttcgagtg atggaatccc gtgctttttc aactaaccct
    44881 atttaacggc gagcacatgt taagacaaaa agacgaaaaa ccgttgcagg aggggacaga
    44941 tgaaacaaga aatgatggaa atcgttcagc aaaaacagct ggcaccaaag atttttgaaa
    45001 tgacattgaa aggtgatcta gtaaaagaaa tgcagcaacc aggacagttc ttgcatattc
    45061 tcgtgccgcg tgcagacttg ctattacgcc gaccgattag cttgaatcat atcgaccacg
    45121 aaacaaatac ttgccgtatc atttatcggg tggaaggtga ggggactaga gtcttctcta
    45181 ctctttctgc cggagaccat ttagatgtca tgggtccttt aggcaatggt tttgatttgt
    45241 ccaatcttca tacaggagac gaagtattta taatcggtgg aggaatcggt gtccctcctc
    45301 tatatgagtt atcaaaacag ttagtcgaaa aaggaattaa gccaacgcat tttttaggtt
    45361 ttgccacaca tgaagtcgtt tattatgaaa aagaatttaa tcgtctagga gaaacaagaa
    45421 tcgctactga cgatggaaca tacggtatgc acggaaatgt cggtaattta ttgttaggaa
    45481 cgaagacgga acctacagct gtttttgcat gtggttcaaa cgggttattg aagacagtgg
    45541 aacagctgtt ttcttcgcat caaaatgtcc agttatcatt agaatcccgc atggcatgtg
    45601 ggataggggc ttgctatgct tgcgtttgtc atacgccgga agatgaaaca ggaacaaaaa
    45661 gtgtgaaagt ttgtgacgaa ggaccgattt ttaaaatagg agaggtaatt atatgaatga
    45721 gaatccttta gcggtcaaac ttcctggcct agaattaaaa aatcctatca tgccggcaag
    45781 cggctgtttc ggttttggca aagaatacgg aaaatactat gatttgaatc agcttggatc
    45841 gatcatggtc aaggcaacga ctccaaatgc ccgctttggc aatcccacgc caagagtggc
    45901 ggaaacacca tctggcatgt tgaatgcaat tggtttgcaa aatcctggtc tagacgttgt
    45961 tatgcataaa cttcttccag aattagagga gtacaatgag ttacccatca tcgcaaatgt
    46021 tgctggtgct tgtgaagaag actatgtaga agtttgtagc aaaattggcg aagcaccaaa
    46081 tgtgaaagca atcgagctga acatttcttg cccaaatgtc aaacatggcg gaattgcttt
    46141 tggaacagat tctgatgttg cttttcaatt aacacaagca gtaaagaaag tttcttctgt
    46201 tcctgtctat gtcaaattgt ccccaaatgt aacagacatc gttccaatcg ctcaggcaat
    46261 cgaagctgga ggggcagatg ggtttacgat gatcaatact cttcttggta tgcggattga
    46321 ccttaagaca agaaaaccga ttttagccaa tcaaacaggt ggactctccg gaccagcaat
    46381 caagcctgtc gctattcgtt tgattcacca agtagcaagt atatccagcc ttccaatcat
    46441 tgggatgggc ggcgtgcaga cagtggatga tgttcttgag atgtttatgg ctggagcaag
    46501 tgctgtagct atagggaccg cgaatttcac cgatccgtat atctgtccta aactaattaa
    46561 agagctccct gtgcgaatga gcgaactagg catcgaatcg ctggaaagat taatcaaaga
    46621 agtgagagag gaacgtgaac gatgaatcaa ctagaccaac gaccaattat tgcattagat
    46681 ttttcaacta ggcaagaggt agaagacttt cttcgatttt tcccgaaaga agaaaaattg
    46741 tttgtgaaaa tcggaatgga attgttctac caagaaggac ctgaaatcgt acgttatcta
    46801 aaagaggctg gtcataatgt atttttagat ctgaaacttc acgatattcc gaatacggtc
    46861 gaaaaagcaa tgcggggatt ggcaaagctt ggtgttgatc tcacctgtgt tcatgccgct
    46921 ggcggtattc gtatgatgga ggcggcaatg cgaggattag aagaggggac tccagaaggt
    46981 ggaaaacgtc cgctacttct agctatcact cagcttactt ctacaagtga agaagaaatg
    47041 catgccgatc aactaatcga agtttcttta gaaaaaagtg tgatccatta tgctagctgt
    47101 gccaaaaaag caggattaga tggtgtggtt tcttctgctt gggaagtaga agcgatcaaa
    47161 gaaatggcag gagacgaatt tgtttgctta actccgggta taagaccaga aggaacagtc
    47221 gcaggagatc agacaagagt agtcacacct tctcaggcaa aaaagatcgg ctcaactttt
    47281 atcgttatag gtcgaccaat cactcagtca actaatcctt ataaagcata tcaaacgatt
    47341 caaacagaat ggagctaacc taaaatgaat gttgaacaaa gtatcgcaaa agacttatta
    47401 gaaattgaag cagtattttt aaatccaagt aatcctttta catgggcaag tggaatcaaa
    47461 agcccaattt actgtgataa ccgaatcacg atgagctatc caaaagtccg aaaagagatc
    47521 gcaaaaggat tagcaagcaa aatcaaagaa gcttaccctg aagtacaagt gattgctgga
    47581 actgcaacag caggaatccc tcatgccgca tgggtagcgg aaatactgga tttgccaatg
    47641 gtctatattc gaagtaaggc gaaagatcat gggaaaggta accaaatcga aggacgtata
    47701 tttgaaggtc agaagatggt tgtgatcgag gatttgattt ctacaggcgg aagtgtcttg
    47761 gaagcagctg aagcagcaaa aagagaaggt gcggatatcc ttggtgttgc ggctatcttt
    47821 acgtatgagt tgccaaaagg aaaagcaaat ttcgaaaaag ccgaaatccc tcttatgacg
    47881 ttgaccaatt attctgtatt gattgaagca gctttagaag atcgttatat cgatgagcaa
    47941 gaattaactc tgctaaaaga atggaaaaaa gatcctgaaa attggcagat ttaagcgcgc
    48001 ttgatgaaga tagagaaatc cgatttgtgt ctccagaatc atcaattgga atagcaaaaa
    48061 tccaaaaact gattgcgaga agcatctttt catcaggtat gaaaagaaac ttctatcgtg
    48121 atcagttttt gattttttta aaattcattt ttctaagact ggttagcaga aagaagagaa
    48181 taat
//
LOCUS       DACDYI010000013        45268 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.013, whole genome shotgun sequence.
ACCESSION   DACDYI010000013 DACDYI010000000
VERSION     DACDYI010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.013"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..1046
                     /locus_tag="IWM52_000706"
     CDS             <1..1046
                     /locus_tag="IWM52_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9972981.1"
                     /translation="LKLAILLIIFWAILLSQLKGTKYTSLIARIGFVAGILLPALVLF
                     GLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVETSASHANEMENP
                     KKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLEFLLRHIDPNLAW
                     IAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENTHGVPAHLMIANG
                     IVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKLVFKDDSLERAYQ
                     VPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILIVSYLIVLVIPFT
                     IYACRHKFQHAEK"
     gene            1069..1995
                     /gene="glsA"
                     /locus_tag="IWM52_000707"
     CDS             1069..1995
                     /gene="glsA"
                     /locus_tag="IWM52_000707"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAP9972982.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            2203..3663
                     /locus_tag="IWM52_000708"
     CDS             2203..3663
                     /locus_tag="IWM52_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972983.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            3884..5647
                     /locus_tag="IWM52_000709"
     CDS             3884..5647
                     /locus_tag="IWM52_000709"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAP9972984.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            5731..6189
                     /locus_tag="IWM52_000710"
     CDS             5731..6189
                     /locus_tag="IWM52_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP9972985.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            6298..6981
                     /locus_tag="IWM52_000711"
     CDS             6298..6981
                     /locus_tag="IWM52_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP9972986.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            7128..7742
                     /locus_tag="IWM52_000712"
     CDS             7128..7742
                     /locus_tag="IWM52_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAP9972987.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            7758..7964
                     /locus_tag="IWM52_000713"
     CDS             7758..7964
                     /locus_tag="IWM52_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972988.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            8505..9635
                     /locus_tag="IWM52_000714"
     CDS             8505..9635
                     /locus_tag="IWM52_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP9972989.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            9727..10482
                     /locus_tag="IWM52_000715"
     CDS             9727..10482
                     /locus_tag="IWM52_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP9972990.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            10540..12579
                     /locus_tag="IWM52_000716"
     CDS             10540..12579
                     /locus_tag="IWM52_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAP9972991.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(12688..13839)
                     /locus_tag="IWM52_000717"
     CDS             complement(12688..13839)
                     /locus_tag="IWM52_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP9972992.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(13993..14523)
                     /locus_tag="IWM52_000718"
     CDS             complement(13993..14523)
                     /locus_tag="IWM52_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9972993.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(14622..14972)
                     /locus_tag="IWM52_000719"
     CDS             complement(14622..14972)
                     /locus_tag="IWM52_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAP9972994.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(15129..16658)
                     /gene="ahpF"
                     /locus_tag="IWM52_000720"
     CDS             complement(15129..16658)
                     /gene="ahpF"
                     /locus_tag="IWM52_000720"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAP9972995.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(16668..17231)
                     /gene="ahpC"
                     /locus_tag="IWM52_000721"
     CDS             complement(16668..17231)
                     /gene="ahpC"
                     /locus_tag="IWM52_000721"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAP9972996.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(17375..18037)
                     /locus_tag="IWM52_000722"
     CDS             complement(17375..18037)
                     /locus_tag="IWM52_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9972997.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            18221..19867
                     /locus_tag="IWM52_000723"
     CDS             18221..19867
                     /locus_tag="IWM52_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP9972998.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            19858..20940
                     /gene="uxuA"
                     /locus_tag="IWM52_000724"
     CDS             19858..20940
                     /gene="uxuA"
                     /locus_tag="IWM52_000724"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP9972999.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(21048..22601)
                     /locus_tag="IWM52_000725"
                     /pseudo
     CDS             complement(21048..22601)
                     /locus_tag="IWM52_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
     gene            22749..23890
                     /locus_tag="IWM52_000726"
                     /pseudo
     CDS             22749..23890
                     /locus_tag="IWM52_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(24016..25095)
                     /locus_tag="IWM52_000727"
     CDS             complement(24016..25095)
                     /locus_tag="IWM52_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9973000.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(25198..25638)
                     /locus_tag="IWM52_000728"
     CDS             complement(25198..25638)
                     /locus_tag="IWM52_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9973001.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(25695..26105)
                     /locus_tag="IWM52_000729"
     CDS             complement(25695..26105)
                     /locus_tag="IWM52_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP9973002.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(26285..26719)
                     /locus_tag="IWM52_000730"
     CDS             complement(26285..26719)
                     /locus_tag="IWM52_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP9973003.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(26819..27667)
                     /locus_tag="IWM52_000731"
     CDS             complement(26819..27667)
                     /locus_tag="IWM52_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9973004.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            28056..29822
                     /locus_tag="IWM52_000732"
     CDS             28056..29822
                     /locus_tag="IWM52_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAP9973005.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            29924..31183
                     /locus_tag="IWM52_000733"
     CDS             29924..31183
                     /locus_tag="IWM52_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9973006.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            31330..32631
                     /locus_tag="IWM52_000734"
     CDS             31330..32631
                     /locus_tag="IWM52_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9973007.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            32634..33485
                     /locus_tag="IWM52_000735"
     CDS             32634..33485
                     /locus_tag="IWM52_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9973008.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            33498..34313
                     /locus_tag="IWM52_000736"
     CDS             33498..34313
                     /locus_tag="IWM52_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAP9973009.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            34464..35630
                     /locus_tag="IWM52_000737"
     CDS             34464..35630
                     /locus_tag="IWM52_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9973010.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            35860..36507
                     /locus_tag="IWM52_000738"
     CDS             35860..36507
                     /locus_tag="IWM52_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAP9973011.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            36591..37535
                     /locus_tag="IWM52_000739"
     CDS             36591..37535
                     /locus_tag="IWM52_000739"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAP9973012.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            37670..39121
                     /locus_tag="IWM52_000740"
     CDS             37670..39121
                     /locus_tag="IWM52_000740"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAP9973013.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            39142..39978
                     /locus_tag="IWM52_000741"
     CDS             39142..39978
                     /locus_tag="IWM52_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP9973014.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            39978..40637
                     /locus_tag="IWM52_000742"
     CDS             39978..40637
                     /locus_tag="IWM52_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9973015.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            40763..41452
                     /locus_tag="IWM52_000743"
     CDS             40763..41452
                     /locus_tag="IWM52_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973016.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            41458..42414
                     /locus_tag="IWM52_000744"
     CDS             41458..42414
                     /locus_tag="IWM52_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAP9973017.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            42458..42970
                     /locus_tag="IWM52_000745"
     CDS             42458..42970
                     /locus_tag="IWM52_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9973018.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(43226..43774)
                     /locus_tag="IWM52_000746"
     CDS             complement(43226..43774)
                     /locus_tag="IWM52_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9973019.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(43863..45044)
                     /locus_tag="IWM52_000747"
     CDS             complement(43863..45044)
                     /locus_tag="IWM52_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAP9973020.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
BASE COUNT        15204 a         8042 c         8719 g        13303 t
ORIGIN      
        1 atctgaaatt ggcaatccta ttgattattt tctgggctat tttactctca cagttaaaag
       61 gaaccaaata cacttcattg attgctcgga tcggttttgt agctggtatc ttgttgccag
      121 cattagtctt gtttggctta gggatccatt atgtcgcttc aggcgctcca ctacaaacga
      181 tactgtctat gaaaacattg attcctgatt tttcgaaatt gcctacgctg gttgtttttg
      241 tttcctttat tttagcatat atgggcgtag aaacatctgc cagtcatgca aatgaaatgg
      301 aaaaccctaa aaaggattat ccattagcaa tgtttgtatt ggttattctg gcaatcattt
      361 tagatacttt tggcggattg accgtcgcta ctacagtacc tcaacatggt ttgtctctta
      421 atacaggggt gattcaatcg ttagaattcc tattaagaca cattgatcca aatcttgcat
      481 ggattgcgaa aattcttgcg attcttgttt gtttaggtgt catcggagaa attgcttctt
      541 gggtaacctc tccttcaaaa gcacttcacg tagcagcaga agaaggatta cttcctgaat
      601 attttgcaaa agaaaacaca cacggtgttc ctgctcattt gatgatcgca aatgggatcg
      661 ttgcaacaat ctgggcagcc gtctttacat taagcggcgg ggggaataat ctttcatttc
      721 ttgcagcgat gtcattgact gttgtaatct acctgatgtt gtactttatg tttttcgcgg
      781 gctatttgaa gctggttttt aaagatgaca gtcttgaaag agcctatcag gttccaggcg
      841 gaaaagttgt aaaagtaatc gttgcttgtt taggtctttt gacttcactt tttgcttttt
      901 gtatctcatt tgttccacct gctagtatcg cagctggaca gcatgggatt tatgaaacga
      961 ttctgattgt ttcttatctt atcgtcttag ttattccatt tacgatctac gcatgccgtc
     1021 ataaattcca gcatgcagaa aaataaaaaa tgaaagctgg ttattcaaat ggaagtaaaa
     1081 gaattagaac aattgattgg aaaaaatttc cactattatc aaacaggaca agtggcggat
     1141 tacattccag cattagctga agtggatccc aaacaattag gtatggcgat ttatgatttg
     1201 gatacaaaac aactgattga gggaggagac gccgatgtgc gctttgccat cgaaagcatg
     1261 tcaaaagttc ccgtattgtt attagcaatc aaagacaacg gaatagaaaa agtctttcaa
     1321 acaatcaaca cggaaccaac cggttttgcc ttcaattcta tcatgaatat ggaaatcaat
     1381 catcgcaaac atccaatgaa tccttttgtt aatgcaggag cgattgcaac aacctcgttg
     1441 attgccggta aagatgcaga tgaaaaattt gagcgaatct tgtcatttat gaaagagatc
     1501 tgtgatgatc ctaatatcac attgaatgaa gaaatctatc attctgaatc aagaacaggt
     1561 gatatcaatc gctctcttgc ttactatatg aaaggtaatc aaatgatcga aggagacgta
     1621 ccggagatac tcgatgctta tttcagacaa tgctccctca atgtcaccgc tacaggcata
     1681 gcaaaattag ctgctgtgct ggcaaataaa ggaattgctc cttggaatgg caaacaaatc
     1741 attactgaag aaagtgctac aatcgtcaaa tcaattatga caactgctgg tttgtatgat
     1801 gaatcaggag agttttccgt ccatgttggt gtacctgcaa aaagcggtgt aggcggcggg
     1861 ttgatggcag ctgtgccaaa tcgttacggc ataggtgtct tcagcccggc actagatcca
     1921 tttggtaaca gcgcagctgg tatccaattg ttgaaagatg tagtcaaaga actagacgct
     1981 gacatctttg aataaccatt cttgaaagag gctggggtaa aagtccaaac ggctcaaagc
     2041 tacctacaat gaggctgaaa ttgtgagctt gtttgacttt tgtcccagtt tcttttttta
     2101 ttggtttttg aaagggcttt tctaaataaa aaggaaacaa aaatacgatt tacagatatg
     2161 attaaataaa atcataagaa aaactggagg cgtgtatcag gaatgctgga aaattacatc
     2221 gagcgaaata tatttagaaa agtgtatctg tgtgaacaat tatttgaatt ccaagaaata
     2281 gacatcgaac aaacagctat ttctctaagg gtgactacac caacgatcct tcacgatcta
     2341 gaaagtctag ccgagtgtct agaatattgt ataaaagaac aagtaagaga gaagcataag
     2401 tataaactcg tattcaaaca tggtattgct ctttctgagc tcacacagtt tctgtacggt
     2461 caatcttatt ttttaaagtt tttaagttat tattttaatg gtatattcac gagtacagaa
     2521 ctggctgatc ttgaatttat ttctttgagc aaagtatata caatcaaaaa aatcgtattg
     2581 gattttttta aagaaaatga ttatttgaaa aataaagaaa tcattatccc agaatttgac
     2641 tcgcggaaca tcttgctagc tttagtacgt tatgtcaatt gggaaggcta tgaaaataaa
     2701 aatccgtatg tagaagaagc ttgtaacgag ttgattgatt atgtagagac tcattttttt
     2761 aaaagaagat attcggaaga tgaaaagtcc tttatcatac gaggaataga gatcgcaatc
     2821 ggaagaaagg catttcccct tggactgaaa gaagaagata agaaaagcgc agaaacgaag
     2881 ccgttatttc aattagtctg tcaaggattg gaagagcaaa aaggaattct agacttacaa
     2941 gaagaagatg tctattatat tttttctttg ttcaacagta gaaattatac caatgagaac
     3001 atggagctgt taagaaaaga tttcgaagtt gtttataaaa attttgttct caatgaccta
     3061 tcactgaatg aattgatttt acagattatt agtatcgctg aaatcgacga tgcagaaaat
     3121 tttattttca agcaatcctt tttacaattt gtccgtacgt tgtgggcaga tggacaagta
     3181 tttttaccgg aaaggatcta tcttctatca gaaaaagaac aacttctgta cggaaaaatt
     3241 gtagaaatat tggaagagtg gaaaaagaaa aatcaaatcc aactaagatg gaatgaaaat
     3301 ttcattcgaa aattcgtcaa aagtctttct ttgatcaaca gtgaacaagc ggggagacag
     3361 gaaatagaaa tttttgttgt ttcagaaagt tcagtcaaac aattttttta tcgaacccaa
     3421 tttcatttgt atgcggaaga aggcatcgct gaaataaatc ctattttatt tcgttcatta
     3481 gaagaattgc ctgatgaatg tctatgtgcg aaaaaaagag tggttttgtg cgatgctgct
     3541 agctatcaaa atggattaga aactgaaaag acaaagatct atccaatctc attaaaaggt
     3601 gcaaacatcc atttgtatca tattgctcag gaacttcacc tattaaaaaa acatatttct
     3661 tgatgagacc ttaattagga gaccttctac aaagcttttt aattgtctgt agaaggtttt
     3721 tatttgagtc ctgattcgtg cgaagatcta cttatataac aagctttctc tgaaattttt
     3781 ttaaaatgct attgacttag agtcaactct aagtagtaga atagataaag tgtaacaaat
     3841 ctattttcat taaaagaaga tagtacggag gagagaatgt attatgagta cagtattaca
     3901 tgtattggat gcaatcaata gccccgcaga tgtcaaaaaa ttatctgttg aggaaatgaa
     3961 acagttggca gaagagatgc gccaaaaaat cttgcaaaag gacagcgatg taggtgggca
     4021 cgttggtccg aatctaggtg tagtagaatt aacgattgcg tttcattatg tatttgattc
     4081 accaaaggat aaaatcgttt gggatgtttc ccatcagtct tatggtcata agcttttaac
     4141 tggtcgaaaa aatggttttg aaaaaggaca ttaccgtgaa gtgagcggat attccaacca
     4201 acatgaaagt aaacatgact attttacagt ggggcacaca tctacatcta tcagcttagc
     4261 aacaggttta gctaaagcga gagatctgaa gaaagaatca ggaaatgtgg tagcatttat
     4321 cggtgacggc tccctaagtg gtggcttagc ttttgaagga ttgaacaatg gtggtgtttt
     4381 acaatccaat ctaatcgtat tagtaaacga taatgagatg tctattgatg agaatcatgg
     4441 cagtttatat aaaaacttag ctgagcttcg tcaaaccaac ggtaaaagca gcaataacct
     4501 atttaaagca atgggatttg attatcgtta cgtggataat ggaaacgatc tggaaacggt
     4561 gatccgttta tttgaagaag taaaagatat tgatcatccg attgtacttc acgtccatac
     4621 ggaaaaaggt cgcggttatc agccggcaat cgataataaa atgaaatatc attggcatgt
     4681 accatttgat ctgtccaccg gggaggcaaa gcaagtaact tctggagaaa catttaatat
     4741 gatcatcatg gattatttgg ataagaaaat gcaaacaagt acagctccta ttgccgcagt
     4801 caatgcagcg ataccaggcg cctttggact gaaaacattc caagaaaaac atccagatca
     4861 ttattttgac gtggggatcg ctgaacagca ctctatcact ttcgcttctg ctttggcttc
     4921 tcagggagta cgtccagtcg tcttccaatc aagtactttc ttgcaacggg catatgatca
     4981 attatcacat gatttagcga tcaacgagaa tccagtgatt attttagtcg gtggcggaac
     5041 gatctcagct agtgaccgaa cacaccaagg aacatttgac attccttact tatcaagtat
     5101 cccgaacatc aaatatcttg ctccagcaac aaaagaggat atgatctcta tgctagattg
     5161 ggcgttcgaa caaacagcgc agcctgtagc aatccgaatt cctacacatg gtgtagaaca
     5221 tggtacagct atcgcaaatg attatagtat tccagcctat caaacagtaa ataatggagg
     5281 aaaagtagca attttagctt taggcggttt tcttgaatta ggaaaagaaa cagtaaaagc
     5341 ttttagaaaa aaaggaatcg aagcaacgtt gatcaatcca ggaacgatca cagaaaatga
     5401 cgtagaaaca ttagataaat taaaagaaac acacgaatta gtcatcacat tagaagatgg
     5461 tagtttgtca ggaggatttg gcgaaaatat cagtcgttat tatggtgatt cttcaatgaa
     5521 cgtattaaat tttggtgcag agaaagaatt cacggatagc gttcctgtag ggcagcttta
     5581 tgaacgttat catttaacac caaaacaaat cgttgaagat agtctgcgtg tcttgaatat
     5641 tcaataattc attatgctca ccatccagat aattgtttat actagtatct ggatggtttt
     5701 ttcattataa aaagcaaagg ggtggatgtt atgtatcagc aagtagaaga atatgtcaaa
     5761 aagttccaag aaaaatatcc tagttatcaa tcagaaaaat taggtttctt ttcatttggg
     5821 gatacagaaa aaatggcaga cgagttgagc gaactagttg taaaaaagat aaagactggt
     5881 acatcaagtg gttacgatct gtataaacaa gagaatgagc ctttaccaaa agttggtcaa
     5941 ttagacgtgg tcctgaatgg aaaaaatgaa ccagtttgta tcatcaaaaa tataaatgtg
     6001 accatcttgc cttttagtga agtcacgaaa gagcatgctt ggaaagaagg agaaggggat
     6061 cggacattag cttattggcg gaaagtccat gctgagtttt ttaaacatgc ttataaagaa
     6121 gcgttgaaca tagaatttac agaagagaag ctggtcgtat acgaagaatt tgaagtaatt
     6181 ttcttttagt aagaaaggtc tgctgaaaga aatagctcat tattgtctcc tgaatagaaa
     6241 agggttacat ttttatatga agctgtattt ttatataaca ttactggagg agatattgtg
     6301 gatacactgg aacaaatcaa aggcggagtc gtcgtttctt gtcaagcttt ggaaaacgag
     6361 ccgctacaca gttcttttat tatggggaga atggctttgg ctgcaaaaga aggcggagca
     6421 gttggtattc gagcaaatac gacagaagat atcaatgaaa tcaagcgaat cgttgattta
     6481 ccggtaatag ggattatcaa aagagattac gatgggtcag aagtgtttat tactgcgaca
     6541 gaaaaagaag tggaagaact tttggagacg caagcagaaa tcattgcact ggatgcaact
     6601 tctcgtaaac gtccgacaga aaaaacaact gctgaacttg tccaaatgat tcacgaacac
     6661 ggtcggttag ctatggcaga tatttcgact tttgaggaag cgcttcaagc gcaagaagat
     6721 ggttttgatc ttttgtctac gactttagca ggctatacag aatattcacg aaagacgcag
     6781 acacctgatt ttgatttatt gaatcaaatc atagaacaag tcgatatccc agtaattatg
     6841 gaaggacata ctgactctcc tgaacaagta gagaaagcat atgaattagg tgctttttca
     6901 gtcgtagttg gatctattat cacccgtccg caattgatca ctaaaagata tgtcgaagcc
     6961 gcaagtaaag caaataacta aaaaacacgc acggcacaga ttcttgacaa aaaagaaaaa
     7021 ttgatagata gtttaaagca gattctaaga agatgaaaaa agcattactt ttcattttgt
     7081 cttcaagatc tgctttttaa cgaattttat agaaagaagg cgaaaccatg tttgaacgga
     7141 gtccttcaga attgacagaa cgtgaaaatt acaagttgct gattggttca gtcatcccta
     7201 gaccagtagc agtagtaacg actaaatcag caaaagatat agtgaatatc gcacccttca
     7261 gttacttcaa tatcgtcagt tcgaatcgac ccatcttatc cttggcgatc cagagaaaac
     7321 aagatgcagt aaaagacaca gcaaagaata ttcttgagac aagagaagct gttatccata
     7381 tccttgatga ggacaacgta gaagaagtaa ataaaacagc agccaatctg cctaatgagg
     7441 aaagcgaact gtacctgact gacttgactt taactgattc agtaatcatt tcagtaccag
     7501 cattgacaga agcaaaagtt cgttttgaaa catccttata tgaacatgtc gagatacgga
     7561 ataaaaataa agtaacagct gatttatttt tgctagaagt acagaaatat catttaaatg
     7621 aaacagtcta tgatgataag acaggaagga tcgatccaag aaaattgaat gctgtaagcc
     7681 gtttggcagg aaatgattat gcagcaatag gagaaatttt cacgattgca cgtcccgaat
     7741 aaaaaggagc actagttatg ttaaaaataa gctataaacc atcgacagat tcaaaagaaa
     7801 tgaaaaaaga atatgaaaca gtgaatgatt ttctgcaagg gcagtactta gaagttccgc
     7861 cattgcaaga ccattttgtc gttactactg ttactttaga tggtaaggaa atagaaatgc
     7921 cagatcaaac gattagcgga ttgtttaatt actttaacaa ataaatattt attccgaaaa
     7981 gtgaaaaaac atataaaaaa acacataaaa ctaaaaaatt atcaaagaat cctggtgcaa
     8041 ctgaaagaat ttgcaacggg attctttcaa cttatttgtt atttttttct aaaaaatatt
     8101 aaagattgtt aaatttcact atttcatttc aaattccatt gcctaatcat taggaaaagt
     8161 ctatcatgaa ctcgtaaata gaatatagta ataaccttgt taggtgaggc tcctatacaa
     8221 acataggctg ttgcccagaa acgtcgaaag tcgccaatgg gtaaaacaga aactgttgga
     8281 ttaaggcttt ttccaatagc taagaatgtt ttctttacgt tgtatagtgc taaaactcgg
     8341 acgatgagat gatgctaggt cagaattctt gttcgaaaag gtttctgacc ttttctttat
     8401 atttttgtga ctgacttatt tgatgagaca aactcgctcc tcacctgaca agtggattgc
     8461 tgtgtgagga ggagatgcgg tgaaaaaaga tcgattggtg gattttgggt ccttatttga
     8521 ctttgtccat gatcggtctt ttagaagtat acagtgccag ttcttatcgt ttattgcaag
     8581 ccgatgaaaa tacaaaaagt ctattattgc gtcaactaat tttcatattt ttgagttgga
     8641 gcgtgatctt cttagctcgt tcagtcaaac tacactattt acttcaccct aaaatagcag
     8701 gatacggttt agccttatcg attttctttt tagtattagt aagaataggg atattcggtg
     8761 tcactgtcaa cggcgcacaa cgttggatct ctctgtttgg cattcaattc cagccttctg
     8821 aactggcaaa tctttttttg attttttatt taagctggtt ttttcgtgac ggaaatagta
     8881 gcccaaaaga tctaaaaaaa ccattcctga ttacagtagg tataactttt ctgattttat
     8941 ttcagccaaa gattgctgga gcattgatga tcctttcgat tgcgtgggtc atattttggg
     9001 cagcggcggt tccatttaaa aaagggatct atctaatcgt tactttttct gcattgctga
     9061 ttggagcagc aggcggggta ttatatttag gaaataaagg ttggcttcca cagatgttta
     9121 atcatgctta tgaaaggatc gcgactttaa gagattcttt tatcgatagt catggtgcgg
     9181 gctatcaaat gacccattcg ttttatgcac tatataatgg tggcatattt gggcggggat
     9241 taggaaatag tatcacaaaa aaagggtatc ttccagagac agaaacagat tttatttttt
     9301 cgattattac agaagagctt gggctaatcg gtgcattgtg tgtgcttttc ttattgttct
     9361 cattatgcat gagaatcttt tgtttaagca gtcgctgtaa aaatcagcaa gctggattgt
     9421 ttcttttagg attcggaact ttgctattcg ttcagacgat aatgaacgtg ggaagtatcg
     9481 cggggctgat gccaatgact ggtgtgccat tgccatttgt tagctacgga ggaacaagtt
     9541 atctgatcct ttctttagga atcggaatca cgttgaatat ttcttcaaag atacaggccg
     9601 aagagttggc gctctatcga cctgaaaaac aataaagaga aactgagaaa ctctcagttt
     9661 gaattatcat aaagtacaat cttttatttt tcaagcgaga ggttctttta gaaagaggaa
     9721 ttcgcaatga aagtatctca aaaaatcata ttatctattt tgttactgct tacattagta
     9781 gttggctatt tttcgattga gtttatccat ggattttcgt ctgcaaaaca gagttcaact
     9841 gtgaaaaagg tagagccgaa aagtatccct accacactaa atgtggcttt gattggttca
     9901 gatgctcgtt cgaaagacga aaacggtcgc tcggattcac ttctggttgc acaatacaac
     9961 cagaaaacac aacaagcaaa actgatctct atcatgagag attcatatgt cgatattcca
    10021 ggttacggaa tgaataagat aaatgcagct tactcttatg ggggagttga tttattaaat
    10081 caaacattaa aggaaaattt caaatttgaa gctccctatt atgcaagtat cacatttcaa
    10141 gattttatcg attgcgttaa tgaactgttt cctggtggag tcaagatcga tgcagaaaaa
    10201 tctctagatt tagatggcgt atatataaac aaaggagagc aagtgatgga tggtaatact
    10261 ttactccagt atgctcgatt tcgggaagac aaagaaggag acttcgggag gatcagaagg
    10321 cagcagcaag tgatcaaagc tatctctcag cagctgaaag acgtaacttc aatattcaag
    10381 ctacccaaag cggttgggaa attacttggt agtatccaaa cgaaccttcc agaaagtgtg
    10441 cttctggatt gtggtatgga tttcctcaaa gacatatgtt agaataaaca ggtataaata
    10501 gtgaaataaa ggaatgacaa gcaagagaag gaggaaaaaa tgaaaagaag tgacaagcac
    10561 ggcaaaaatc gaacaggcgc ttatattgcc ggcgcagtga tcttaatagc agctgcgggt
    10621 ggcggttatt tttactacca gcactatcaa gaaacccaag cagtagaagc tggagaaaag
    10681 acagttgagc aatttgtcca agctttgaac aagggagatt ataacaaagc tgcagaaatg
    10741 acatcaaaaa aggcggcaaa taaaagtgca ttatctgaaa aagagatctt agataaatac
    10801 cagaatatat acggtgctgc tgatgtcaaa ggacttcaga tatcgaatct aaaagtagat
    10861 aaaaaagacg attctactta cagtttttca tataaagcaa agatgaatac ctcattaggt
    10921 gaattgaaag atctttctta taaaggaaca ttagacagaa atgatggtca aaccacgatc
    10981 aactggcaac ccaacttggt ttttccagaa atggaaggaa atgacaaagt aagcctgacc
    11041 acacaagaag cagcaagagg gaatattata gatcgaaatg gtgaaccatt agcaacaaca
    11101 ggcaaactaa aacaattagg agtcgttcca agtaaacttg gagatggagg cgaaaaaaca
    11161 gccaatatca aagcgattgc ttcctccttc gacttaacag aagatgctat caatcaggcg
    11221 atttcacaaa gctgggtaca acccgattac tttgtcccat tgaaaatcat tgatggagca
    11281 acgccagaac ttccagctgg agctaccatt caagaagtag acggcagata ttatcctttg
    11341 ggtgaagcag ctgctcagct gattggttac gtgggagata tcacagcaga agatattgat
    11401 aagaatccag aattaagcag taatggaaaa atcggtcgct caggtttgga gatggctttt
    11461 gataaggatc ttcgtgggac aacaggtggg aaattaagca ttacagatgc agacggtgta
    11521 gagaaaaaag ttctgatcga acatgaagtt caaaatggca aagatatcaa attgacaatc
    11581 gatgcgaagg cacaaaaaac agcttttgac agtctaggag gaaaagctgg atcaactgtt
    11641 gcgacaacgc caaaaaccgg tgatcttctt gcgcttgcta gctctccaag ctatgatcca
    11701 aacaaaatga caaacgggat ctcacaagaa gattacaaag cttatgaaga aaatcctgaa
    11761 catccattca tcagccgatt tgcgacaggt tatgctcctg gatctacgtt taaaatgatt
    11821 acagcagcaa tcggtctcga caacggcact atcgatccaa atgaagtgtt gacgatcaac
    11881 gggcttaaat ggcaaaaaga cagttcttgg ggatcttatc aagcaacgcg tgtcagtagt
    11941 gatgtatcac aagtagactt aaaaactgct ttgatctatt ccgataatat atatgcggca
    12001 caagaaacgt tgaaaatggg tgagaaaaaa tttcgtacag gcttagataa attcattttt
    12061 ggtgaagacc ttgatttgcc aattagtatg aatccagcac aaatttctaa tgaagatagc
    12121 tttaactcag atatcttgct agctgatact ggatatggac agggcgaact tctaattact
    12181 cctatccagc aagcagcaat gtattctgtt tttaccaaca atggcacact tgtctatcct
    12241 aaattgattg cagataaaga gacaaaagat aagaagaatg tcatcagcga aacagcagta
    12301 caaacgatcg tgccagatct gagagaagtt gtgcaagatg taaatggtac agcacattct
    12361 ctttctgctt tagggattcc gttggcagcg aaaactggta cagcggaaat caaagaaaaa
    12421 caggatgtaa aggggcaaga gaacagtttc ttgtttgctt tcaatcttga taaccaaggt
    12481 tatatgatgg tcagcatgtt ggaaaataaa gaagatgatg attcagcaac caaacgagca
    12541 tcggaactat tgcaatacct caaccaaaat tatcaataaa aaattgcttt ttaagtacag
    12601 taaaaggact gtggcgttat cgtcacagtc cttttttcaa ataaacggtt ttcacgatgt
    12661 ctctttcaca acttcttttt gatttactca cacttcataa actttttttg taaaatattt
    12721 gagaacaagt ggagaaataa gtgtacttaa taaaatcacg ataactaaag gtgaatagta
    12781 ctgtggttcg atcaattgac tttgctgtcc aatttgtaaa atgatcaaag ccatttctcc
    12841 acgcgaaatc ataccagcac cgaccatcaa tgaactatgt tgagaaaaac cggcaatctg
    12901 agcaccaaaa taacctccaa gcaatttggt caaaatcgct acaagcgtca atatagaaat
    12961 aaagagtagt tgttcactaa agtcagaaaa atcaacttct aatcctacac tgacaaaaaa
    13021 tactggtata aatactgcat atcctaaagc ctctacattg tggtatactt cttctttaac
    13081 ctttgtctga ctgacagcta tcccagcaaa aaaagcacca atgacagagc taagcccaac
    13141 aagatccgct acgtaagaca ttcctaaaca aataactagc gatacgatga taatagaaga
    13201 atttgcgtac actttttctg caaatgaaag taggaaaggc gcaatccatt tgactaataa
    13261 gaaaataaaa acaaaataaa ttagctgttc aatcaaaaca agcggtaaag aagcattgct
    13321 agaagcttcc cccgttagaa aagataggct gaaacttaga actaaaacga ctaaaatgtc
    13381 atctaccaca gaagcaccca aaattgttga tccttctttt gtattcacta cgtttagttc
    13441 ttttaatact tctactgaaa tactgacaga agttgccgca agaataatcc caaagaaaat
    13501 cgcttcattt cccgaaactt ggaatgcttt gcctgtcaac cagccaaaaa caaccgggaa
    13561 gatgatccca agcaaagcaa cgaacatacc aggtcggaaa tattttttca aaagagataa
    13621 atcactttcc agaccagcta aaaacattaa taagatcaca ccaatctccg aaaagtcatg
    13681 gactagaata tcaggtttga cccaattcaa tccagcttgg cctagcaaaa caccaacaag
    13741 taattgtcca atcactgctg ggatacctaa tctccgcgag atatgtgagc caatcgttgt
    13801 tgaaatcaat atcaggcata aaataccaat aaattccatt ctcatcattc ctttgtataa
    13861 aatatagccc gaacttagca taacatacat tattgaaaaa ataggaaaat atactcacaa
    13921 aaaaaccaac aatccaaaag atttttttgg attgttggtt tttatacggt ctattttaat
    13981 aactaaaaat gattaacgta tagtcatttt tcttattctg atgaaattga taatctcctg
    14041 gatgcccatg aaaatcaaaa taaagccgaa tatttggaat agaagcatca aacttttgaa
    14101 tggattgaac aataagacga atccgccaat caacaacaga atactactat aaacactcca
    14161 acttttagct tttgtattca tgctgaagaa caactggaac aagctgttga tgataacagc
    14221 taatccaaga agaatcggca ggatagaaac gattgcaggt gcaaaagcat agacgatcag
    14281 agctaagatc aggaaaaaaa gaccactgaa cagaccgata ctccaagaac ctgttttcct
    14341 tttaaaacgg taagcctcaa aaagattcaa caacccaagc aaaacaagat aggcggtgat
    14401 cagataaccg ataaaatgaa aaacagcaga agggtttagc acaatcgcaa tcccagaaat
    14461 aatataaaca gcagctctta ataaggcgta tttttgaata ttttgtttaa tcgtctcaaa
    14521 catataagaa aacctccttt gacttatttt aacgtgaact agtcatttta ccaaattaaa
    14581 tgttacaaaa tgagcgaagg agaagaatat ctctggaaaa atcaacttct gaggatcttt
    14641 tccatttttt tgccttttgc tagctcatcg atcattttat ctaaataacg aatttctttc
    14701 atcaagttat cctcgatgtc ttctactcgt acaccacaaa caacacctgt aatcaaggaa
    14761 cgtgaaggat tggggacagg ggcttccgca aaaaattgtt cgaaagtcac ttgtttttgt
    14821 agttgatatt ctagttctgt ttgggaataa cccgtcagcc aacaaatgat ttcatctact
    14881 tcctctttta tacgatcttt tctctccgct tttgctacat acataggata aacgcttgca
    14941 aaacttgtcg taaaaatacg attttgcgcc ataatcagtc acctttcttt ttttcaaatt
    15001 atttactact tatagtatat aagaaaaaga gaagaaaaag cgattattca aaaaaacaaa
    15061 aaggattgtg acaaaattct tgtcaccatc cttttttccg ctctctgact ttttacaatc
    15121 ttcatagctc aagtgcgcat cagataatcg aatgctccta aagctgcagt agctcctgaa
    15181 cccattgaaa caatgatttg tttgtatgga ctatctgtac aatcaccagc tgcgaaaacg
    15241 ccggggatat tcgttgcgcc atttttctcg gtcactattt catttcttgg attcatttcg
    15301 atcgttccat ctagccaatc cgtattaggg agtaagccga ctagaatgaa tactcctgaa
    15361 acagctagtg tatgcatctc catcgtacgg cgatcgatat aggagagaga ttctacctga
    15421 ttcgtaccgc tgatttcttt cgtcgctgca tgtttgataa cagtgacatt cgcaagagag
    15481 actagtttat cctgtaagat ttgatctgct tttaattctg gtaagaattc tagaacatat
    15541 acatgttttg ctaacccagc caaatcgatt gctgcttcaa caccagaatt accaccaccg
    15601 ataacagcaa tctcttttcc tttaaataaa gggccgtcac agtgtgggca ataagcaatt
    15661 cctttatttt taaactcttt ttcgcctggt acattgatag aacgccagtg cgcacctgtc
    15721 gcgatgatgg cggtttttgc tttaagtgat ataccgttag ctaagccaat attaataaaa
    15781 tgttgctctt tatttaagga aacggcctgt tgattcatca taatgtcgat agggtaacta
    15841 cgcacatgac tttctacttg ctgcatcaat tttggacctt ctgtgtaaga ggtaccaatc
    15901 atattttcaa tccctagtgt ttcaacgact tgtccgccga aagaatcagc gacaagtcct
    15961 gtacggattc ctttacgtgc tgcatagata gctgcactac tagctgctgg tccaccgcca
    16021 atcactaata catcataagg ttctttttca aacaactctt cttcaatcaa agggccagtg
    16081 attttttcaa caatttgttc gatcgtcatg cgtccgctac taaattcttc tccatttaaa
    16141 aacacagttg gcactgccat gattttttta tcatcgattt ctttttggaa catcccacct
    16201 tcaatcatcg tatgagtaat atttggattt aatacagcta atatattcaa tgcctgtaca
    16261 acgtcggggc aattatgaca agtcaagcta acataactgt caaaatgcaa tgtttgtgtt
    16321 atttttttga ttttaagact caaagattca ctgattttcg gtgcccgtcc actaatttgt
    16381 aacatagcca gtaaaaaaga ggaaaattca tgaccgaggg ggataccagc aaaaacgatt
    16441 ccactttctt tgccaactgg attgatagaa aaactaggtg tgcgcatcga atctcttttt
    16501 tcccaagaga ttttaggaga catttctgct atttccttcg taaattcagc tagtttttga
    16561 ctattttcac tattatccaa actaacagaa aaaacaatag gagattcaat cagtgctaaa
    16621 tattgttcga gattttgttt agtttctttg tctaacatat ccaccaccta aatctttcca
    16681 acaagatcaa aacttggttt caatgtttcg ccactttctt tccattttgc cggacaaact
    16741 tcacctgggt gggtacgtac atattgagca gcgcgaatct tatcaatcag tgcactagcg
    16801 tcacgaccga ttccatctgc attgatttcc atagcttgaa cgattccgtc tggatcaata
    16861 ataaatgttc ctcgctgagc taatccttgc tcttcatcca aaacatcaaa aatacgagaa
    16921 atctgatgag acggatcacc gatcatcacg tattcgattt taccgatagc atctgatgta
    16981 tcatgccatg ctttgtgagt aaagtgggta tcggtagagc aagaaaagac ttcaacacct
    17041 aaagatttca atgtactgta ttgttcttgt aagtcttcta attcagtagg acaaacaaaa
    17101 gtaaaatcag ctggatagaa gcaaagaata ctccagttcc ccataagatc tttttcactg
    17161 acttctaaaa attctccttg atgataagca tgtgcattaa attcttcgat ttttttccca
    17221 atcaatgaca tagtaaaacc tccaataata attatttatt tataattatt gtaatttagt
    17281 tggagttgca aagtcaatgg atattttgtt atttaataaa gattactcaa aaacgaaggt
    17341 taattataaa aatagaagag gtcgcaagaa aatgctactg ctggaaatac tcgggataac
    17401 gttgagtaac agtttcctgt tctttcagca tcatgtgtaa gtgcaatccc gtttgaaatc
    17461 gagcttcttc tggtttttta tcaactaaag ccttgagtaa atcataatgt tgttccatga
    17521 tcgtttccca tggaagcccc tgaatggtca ctcgtaacca gcggaaacgt tctaaatggg
    17581 tattatggtc atctaaccag ttccaaactt cttttcttcc tgcaatttcg aaacaagctt
    17641 catgaaataa attatctgca cggaaaaaag cgcgttcgtc atttattttg actgcttttt
    17701 cttgttcttc taaaatcgtt tctagcacta ttcgtgtttg tgtgtccaat ttggaacaac
    17761 attcaatcat gatttgctct tccaactgtt ctcggacaaa gcgagcattt tctgcagaaa
    17821 caagattaat tttggaaaca tacgtaccac tttgaggtat cgtatacacg agttcttgct
    17881 gacgtagttg aatcaatgct tcgcgaatgg gagtccggcc gatagcaaga tgttcctcca
    17941 gtgccttttc agatattttc cttccaggtt ctaagtcagc atagataatt tgtttacgaa
    18001 tcgcttgata agcttgtgat tgcaagctgc ttttcatctt tattcctcca aaatcatttc
    18061 tatgaacgta gtataacaga agaggtctga aaaacaatct ggtatgtcac attaactagt
    18121 aaatttgtat aaatagtaaa attttggttg acatagcgat ttttattgca taaaattcac
    18181 ttgaatacaa aaactaatat gttagtttag gaggggaaaa atgattgatt taaacaactg
    18241 gaggaacttt atccctgaat tatctcaaaa agaagtagaa atacctcatt ttgaccgtgc
    18301 atcattaaga gaaaatggac aaaaaaatcc tgtctggatc catttcggtg gcggaaatct
    18361 ttatcgagga tttcatgcag aaatcgctca acgtttagcg gatctaggcg aattgtccac
    18421 tggtgtcatt gtttgtgaaa cattcgatga agaaataatt gaaagggctt accaagattt
    18481 tgatgatttg attcttgaag tggtcatgcc aagcagtgga gagttcgtca aacgtatatt
    18541 agctgcgaca gctgacagtc tgtattgtca tccagattgt tctgaacact ttgataagat
    18601 gattcgacac tttgaaaatc ctagtttaca attagttact tttgctatta ctgaaaaagg
    18661 ttatcgattg aaagatacaa ataataaata tataggaccg attgcagtcg atataaaaaa
    18721 tggtccaaag aagccatcac atacaatgag tattcttgct gtgctacttt ataaacgttt
    18781 tttatctgga gcttggccga ttgcacttgt ctctacagat aatttttcac aaaacggtag
    18841 tcaattacgt acttctttgc taacgatcgc aaaagaatgg caaaaggaag gttatgtaca
    18901 aaatacattt atccaatact tatcgaatga agaaattgtt gcgtttccat ggacgatgat
    18961 tgatcgtatc accccgaatc cttccttgtc agttagcaaa atgttggcaa aagagggctt
    19021 ttcaaatatg tcgattttcc aaaccgcaaa aggaaataat attgctccat ttgtcaatac
    19081 agaggacatc cattatttaa tcatggaaga tcattttccc aatggacgtc cgaaattcga
    19141 aaaagctggg gtattcttga ctgatcgtgc aacggtagat aaagcagata cgatgaaggt
    19201 gacgacttgc cttaatcctt tgcatacgtc tttagctatt atgggttgtt tactggggta
    19261 taggagcata gctgaggaga tgaaagatcc agatttatcg ctgctagttt ctgaaatcgg
    19321 gtacaatgaa ggcctcccag ttgtggagtc tccaggtatc attgatccaa agatctttct
    19381 ggaagaagta attactgtgc gcttttctaa tccaaatatt cctaatatgc ctcagcgaat
    19441 cgccacggat acctctcaaa aggtggcgat tcgttatggt gagacgatta aaaaatatat
    19501 ggaaatagaa gttaaagacc cgcagacact tgtattcata ccattgactc tcgctgcttg
    19561 gcttcgatac cttattggca ttgatgatca aggaaaacca ttcatcccga gtccagaccc
    19621 actattgaca gaccttcagc ataagctttc cgatattcaa ctgggtacgc aagatacatc
    19681 acatattcat gagaaaatgg ctcctatttt gtcaaataaa gaaatttttg gtgtagatct
    19741 ttaccaagca gagcttggca gaaaaataga agaaatgttc atcaagatgg tagcaggacc
    19801 aaatgccgta cgaaaaacat tacaggatta cttacaagaa catggaggaa atttatcatg
    19861 gaaatgactt ttagatggta cggatcacag gaagaaaaga ttaccttaga tgaaatcaga
    19921 cagattcctg cggtcaaagg gattgtaggc acattgatgg atataccagt aggagaggta
    19981 tggcctaggg agaggatcaa agaactaaaa gaagaaattg agtctcgtgg gttaacacta
    20041 aaagtaattg aaagcgtcaa tgtgcatgat gccattaaaa ttggattacc ttcccgtgag
    20101 cagtatatcg aaaactataa gcagacaatc cgaaacttgg cagaatatgg aatagaagtc
    20161 atctgctata attttatgcc tgtatttgac tgggtgaaat ctgaccttga ctaccgctta
    20221 gaagacggct catccacgtt agcttttata tctgatgata ttccagatga tccaagagaa
    20281 atcgtcgcgc gcatcgaaca ttccagcaat gaatttggat tgccgggatg ggaacctgag
    20341 cgcttagctc ctatcaaatc cttgcttgaa gcctatgcat cagtagacga agaaaaactt
    20401 cgagaaaatt ttgcctattt tctccaatca atcatcccaa catgtgaaga ggtaggggtc
    20461 aaaatggctg tacatccaga tgatccgcca tactcgattt ttggattagc tcgaatcgta
    20521 aagaatcgag aagatttaga ttggttgtgt cgcatagtag acagccctta taacggaatc
    20581 actctttgta cagggagtat tgcagaagat ccagaaaatg atgtatatgg gattttagca
    20641 gaattttgcc aaagagatcg aatcccattt gcacatgttc gaaatattaa atttttagct
    20701 aatcgagatt tctatgaggc accacataaa tccgaatacg gttcattaga catgtaccgc
    20761 atactaaaaa caatgtatga caacggattt aacggataca tccgtcctga tcatggaaga
    20821 atgatctgga atgagacagg aagaccagga tacggcttat atgatcgagc attaggtgct
    20881 gtttatctaa atggattatg ggaagcaatc gaaaaaggaa acaaaagtaa agataattag
    20941 tgaaaaaaac tgtaatttta ttatgtaaat aagaaggatg ttcaaattct tgatacataa
    21001 atccggaggt tgttaataag cattcggatt tatgtatctt atttggtcta ggaagtaact
    21061 tgattttctt ttttcgattg atccatgagt gcttcttctt ttaattcaaa gatatattct
    21121 gagatcctca taatcacttt tttccagtct tctggtgtga tgattccttt taaaatatag
    21181 atttttttga tatgagaagg caattgtttc aaagggcagt cgctgataac gatgttgcag
    21241 ttctccataa aatcattttg tattttgatc gattcatttg aaaaaatttg ttctatctta
    21301 acgatcagat ctttactgat ataaaaatca cgcgtatagt tcacaccaat cacaattcgc
    21361 ggctgttttt tgtagcgatc atacaaatgt actaggtctt caaccatcca ttgcacattt
    21421 tccttttcta aaataactgg taacaagtgt tgattttgtt ctctgaaatc catataaaat
    21481 tgttcaatgg cggtttggac ttctttcaat acgctatgtt cttgataaag catgaaagta
    21541 ggaaggggcg tatttggttg attgaagtta agaaatccat tataaagttt atttcgtgcg
    21601 aatgccaatg agtaaagaat cttttctttt tcaggtataa acaaatcaaa tacagtacaa
    21661 agggactcca ccatttctct tgaataaaga acatgaggaa cgtttgcttt ttttaaaagt
    21721 ttgtattgct ccaagcttgt ttcttccgat tcgattttcg tgatagaaag tcgagctaaa
    21781 atattcacta agatgcgttg ttcgcgacaa attggcagat taggtcgcaa aatatctaca
    21841 gagagatcaa taaaaaagtc aatatattca tactctgtga attctttttg tacgtctttc
    21901 acagaagttg caggaaaatt ctctttacat atttttaata gaaaatataa tttatctatc
    21961 ataccgctat ttagtcggtc aagtacttct tttttaaaaa aagtattcat cataaaagcc
    22021 actacttgcg gatcttcatc ataaaattct gtagcagtac ttgaaaagat gttccaataa
    22081 atatctaatg ctgcatattg aatttgtacc tcttttccag aaaaagactt tctgccatta
    22141 ttagaaaagg agatagacaa accgtactat gctaaaaatg cattaatctt tttcattttc
    22201 gtatacaaat aagaggcact atagtttgtt tgctgactga aactgataat cgaatttgtc
    22261 tgacagctga acaaagaagt caagatctga taactgactg attcgttgca gtatcttcca
    22321 aatagacgat aatagcagta agccggatca attccttcaa aattcaactt taaaaaaggt
    22381 ttttgcttat caatatgcag ttctgttaga ttgagacgct caaagtcttg ttctaattct
    22441 ttgatgtata gtaaaaaatt tgattcagac atatggaaat gatctgctaa atattgtgta
    22501 gtaataaaga aacgatgttc agataggaaa agaaaaatat ctaattttgt attgcttttt
    22561 ttcttcaaga taaacgtttt aatcgcttct tgagtttgca tgaaagaggc tcctttttac
    22621 ttaaattgtt aaaacttgaa aatattactg tttttttcat aaatttagac aaaaaaacag
    22681 taaacatata ggtagaatct ctactatctt atttcttgca tacaaaaaaa tcaaggagtg
    22741 agagagttat gagatattca accatcttaa tatcaacatt tgcagcctta agcttatcgc
    22801 ttactataaa tcagacagtc cacacagagg agacagaaac agccaaatgg acagaaacca
    22861 agtgggccaa cgtcaatcga attacctttc aggatgaatt ttcatccttc aattcaggag
    22921 tcgcaaaatt aagaaaagga caaacagcga ccatcaaagc aaaaattgac tattcgggag
    22981 acttagcacc tatactacaa tcagctgtta catttgagaa tgttgatccc actttgtcga
    23041 ttggctatga cgagaacact ttgactatcc aacacaagca agcgatattt accttcaccg
    23101 ttacattaaa tcaggacttg ttaaaaccag cgctttttac tgtcaaggta tcagatggac
    23161 taccgaacga tcatcattta acgccgtata gtcagcggca gacaattgag caagattcag
    23221 ccatcgactc aggaggagat cttgttgaag aaccaacaga taaaccagaa aatgaaaata
    23281 agccagaagt accgcctaca gaaaatccag acggagaaca gaaaccggaa atagaacctg
    23341 gagaagaacc cgatacagaa actcaaccag agccagacaa taaatcaaaa cctgaaataa
    23401 cccctggaga aaaaccagat gtaactccag aaccagatac ggattctgga aaccaaacag
    23461 taccggaaac aaatccagac acagataatg aaactgaaaa tccagaaaaa ccagaggtag
    23521 atccagaaga aaaaccagat gtgactccag aaccagatac ggacgcaaga gaccaaggaa
    23581 taccagaaaa aataaacaaa aaaacaattc aagaggatgg aaaaaaagag agtaaaaaat
    23641 caaatctagc aattctaaaa ataaatgaag agcagctgaa taaaaaatca cgaatatttg
    23701 atagcgccca atcagctgaa accttaaaaa gtagcaagga tacaaccttt gctgtcctga
    23761 aacgaaaaat aaacagctac caaaatccgg agagtcacaa aacaaagtta tactgtggag
    23821 cggaatcatt ctattaagta tagcaacaat gctttctgca aaaagattta aacaaaacag
    23881 atctctctaa catttgaata atgtctgtga acgcattttt caaataaaaa gaaatatcca
    23941 gaaaatgacc ccaaaatacc agattgaaat ctgatatttg gggtcgttca tactttatct
    24001 ttcttcatat ttattttaag ggatatctgt ttcttttgga agatggttcg ggatcttttt
    24061 cactttcaat acatctaaaa agaaggtaaa gatcggaatg ccgacaatca agccccaaac
    24121 gccaaagaac cgttcgctaa caagtaagac cacaaatgta taaaaaatcg gcagttctgt
    24181 tttgctggac atcaacttcg gattcagcac ataagactca attagatgga caatcaaaat
    24241 cgtaatcagg atataaacaa catctcggat gcctccttgg gaataagcta tgaaagatag
    24301 aggaatacaa gaaatgataa cacctgctac tgggatcaga cttaaaatga aaatcatgat
    24361 cgctaaactc agcagctgat gaaagccaaa tacagccaag gcgattgtag tgagcaacgt
    24421 atttaaaaga gcgacaacaa attgagcttc taataccaaa ccaaatgtat tgacaaactt
    24481 atcggcaaaa tagtaaatat cttgaaagaa ccaagaaaat tcacttttta aaaacaatct
    24541 tgagaataca attgtttttt tcttttcaat cataaaaaag aaacttaaga tgaatgacat
    24601 agcaaacgcc actgccattt ttcctatatc ctgtaaatag ccaagcgcaa tcgaagcgcc
    24661 attttgaagc tgttcaaaaa aatcgtttcg ctcaatataa gtatggatat attgtaaaac
    24721 aggatctgca tcttttggct gatgctcgta aaaattaata acggactgga tcatgtcaaa
    24781 tgtttgatga ataagcatcg ggatatactt tgtgaatgcc agatagataa ggtaaacgat
    24841 cagtgtataa atcatgatag ctaaaacacc tgtagggatt cggacaaatc gctgaatgat
    24901 ttgaacaaga tgaatcatca aataagtaaa gataaatgtg agcaaaatgg ttgtaatcat
    24961 acttctaact aagaaaagaa caaaaacgac aaataataaa acagtgagac ggcgtatttt
    25021 ttcattcgcc aaaaaacgct gataaacggt caaatcgaca acctccaaat aacaaaaaat
    25081 aaacacgtta ttcattatat gatggaatat gaaaataaga aagagcaaaa agaaaaaccg
    25141 ctaaatgacg ttatcttatg agttggtttt cctcataaaa tcgttcatct agcagtttca
    25201 gccctttttc tggtcttgat gaaaattatc tagcattttt cgggtcagat gttgcagtgt
    25261 atccaattct tcctgttcaa gtggaagata agacttgatc ttttgtggaa taccttctgc
    25321 ttgtttttgc aacgcagcac cttgagcagt cagcgtaaca tttacgattc tttcatcacc
    25381 cgtttcacgc tgacgattaa tataattttt ttctgctaac ttttttagta agggagtcag
    25441 cgtaccagaa tccagacaca aatcatttcc tagctctttc actgaagtct ttttcttttc
    25501 ccataaaact aacatcacaa gatactgggt ataggtaaga ttcagcggct tgaggagggg
    25561 agtgtagtgt ctaatgattt ctttagaggc ggcatataaa ggaaaacata cttgattaac
    25621 taaatataga tcttccattt ttccacctcc gctgttttac tatagtgtat catagcggat
    25681 ttacacaaaa aagtctattc actaactgct ttcagagtaa cttctatatt tccagctgta
    25741 gcttttgaat acggacacac tttatgcgct gcctctaata attcttgcgt ttcttccaga
    25801 gaaagacctt cgatagatcc ttcaatctct acacctaaga caaagctcgt ttcactttct
    25861 gaataaaggg aaacttgtgc actgacagta gaagccccag agatattttt ttgctgtttg
    25921 atcaattcta atgcactgtt aaaacatgca ctgaaaccag ctgcaaacaa ttgttcaggg
    25981 ttggttgcat tttcgacgcg tttaccagga gctgcaatat tcaattcgaa tgtacgatcg
    26041 ggattatgca cttctccaga gcgtccgcct gtgttgatca ttttcgtaga atacattttt
    26101 ttcataaaat taccaccttt aattatattt tatacaattg aattttacac aatataattg
    26161 tggaaagcaa agaaatggat tggcataaga taaataagag aaagcacatg aaacagacgc
    26221 gctttctcga atagcaaaca tatacaaaaa atatcagaga caaaaaataa tctcatcata
    26281 aacattacat atatttctca aaaagttgtt ttctttcttc aaaaattact tcacgatgga
    26341 cctgatgaag catccataaa taaccaaaag gatctctgaa cattgagtta gtcacaccaa
    26401 agtccatcat ttctgtcatt tcttgaatct ctacacagcc atttcgtaat gctttattat
    26461 gtgtgtcctc gatatcagag acgatgatat tgaaccagat cgtatttgga tgttcagcag
    26521 taggagcagt caaaccaaga gacgtgtttt catccagcat atggaatctc gtaccataca
    26581 aggtaaaaat gacttcatta ttgcctctag ggtaatctgt tgcctcaatc agttgtattt
    26641 catcaaagat tgtttgatac agttctagtg ctttcaaact atcatttaat acaaaatcga
    26701 tttccgttcc tttatgcata aagatctcct ctccttttca gtcgaacagt tactagattc
    26761 ttttattata ccagctaaag aaaacgcatt caaaaaaata gcggatttac ttaccaactc
    26821 attcgattgg atgataagta aatccgcgtc ccattacttc tgctacgctg ctgacaatga
    26881 caaaggcact tggatcgatt tcttctatca atggtttcat tcgggaaaat tcatgatcag
    26941 tcacaacgat caataaaacc tcttttgact ttcgattata cccgcctttt gcctctagca
    27001 gggtaaggcc acgtccaatc tcagcactta agcgaaggcg gatgtcttcc aaataatgtt
    27061 cactcatgac cataacagat ttctttcgat tgatacctgt ttcgatataa gtcatgacaa
    27121 ttgaagtgat tgcaatagat aaaatagcat aaagaaactc ttctattcca aaaataaaca
    27181 gattaagaga aacaacgaat gcgtctgtaa aaaataagcc aatcgaagta ttcaagtgga
    27241 aatatttttt aaaaatgagg ggagggatcg tcgtaccgcc gctagaagag ttgtttttat
    27301 ataaaatcgc aactcctaaa gcaaaaatcg cacttccaaa aatgacagac aacaaccgat
    27361 ctgaagtaat cattgtttct ggaactattg caatgattaa aggaaaaacc aagcttccga
    27421 aaagtacttt aaaaaagggt tttttaccta aaaatagaag agctagtacc aacataacga
    27481 tattcaacac taaaataact atagctcggt caaagcctaa agcagcttcc agtaatattc
    27541 ctagaccgct gacacctcca gcagcaatat gatgaggccc taaaaacata ttgatagcga
    27601 ctcctaatat gaagagggca aaaataatct taatgtatgt ccatataaat tgtctcgttt
    27661 ctttcattta ttcacttcct taattcatta ataatactct tatcttatta taacttactt
    27721 ttcaccttaa gttaacttta attaataaaa aaattaggct agtctaaatt attatgtttc
    27781 attcttctag aatatacttc tacgccaaaa actgttaaaa aacttttact ttagaaaatt
    27841 ggagaaattg atcgcttaag ataaattcag gacactaaat agtacaggaa gtagactaaa
    27901 acttttaaaa gttttaagtc tatctatttg gcaagtttaa aaaataaaaa ttcggtatca
    27961 aaaaactgtt accgataaca tttttgaaag cgttgtcttt tgcttctcta tcacttatag
    28021 tgatgacgtc atattaaaac acaaggagga aatcaatgga tacagctgca atttatcatc
    28081 gaccggaaag tgaatatgca tttctatata cgaatacaag atttcgtatt cggcttcgta
    28141 ctaggaaaaa tgatatcaaa aaagtatatg tgctatgtgg agatccatat accataacaa
    28201 ctgaaaaatg gtatcaaaaa caacgaccaa tgaaaaaatg tctaagtacc aatgttcatg
    28261 attattggga aattgaagtg acgagcgaaa caagtcgctt acagtatgcg ttccatgttg
    28321 ttggggaaga taacacggac tgtttttacg gtgatcaagg aatttttcct tatcgagaaa
    28381 atgtgattac tgaaccaaat ttttattttc gaatacctta ttttcatcaa atcgatcgat
    28441 tcactattcc agaatgggta aaagaaacag tatggtatca aatttttccc gaaagatttg
    28501 caaacgggga caaagcaaat gaccccgaac acactttacc ttggggaagt aaagatccag
    28561 accgagaaga ctttttcggg ggagatttgc aaggtgtgat ggatcatttg gattacttga
    28621 cggaccttgg catcaatggc atctattttt gtcccatatt taaagccaca tccaatcata
    28681 aatacgatac gattgattat tatgaggtgg atcccgcttt tggcgataaa aaacttttaa
    28741 aaaacttgat tgatgaagca cataaaagag ggatacgtat catgttcgat gctgtattca
    28801 atcatatggg tgtccattca ccacagtggc aagacgttct caaaaacgga gaaaaatcga
    28861 tctataaaga ctggttccac atccgttttt tcccagtaga ttcttatcag atgactgact
    28921 taccagaaac tgctgaaaat atcccatatg atacttttgc ttttactcca ttcatgccga
    28981 aactaaatac agcaaatcca gaagtgcaaa aatatttgct ggatatcgca acttattgga
    29041 taaaagaatt cgatattgat ggatggcgtc ttgatgttgc taatgaagtc gatcatcatt
    29101 tttggaaaaa attcagagaa gcagttacag aaatcaaacc agacatctat atccttggag
    29161 agatttggca ttcctctcaa gcttggctgc aaggggatga attccatgca gtgatgaatt
    29221 atgcatttac agattcgatt aaagattatt ttgcaaaaaa gaaaatcaca gcttcacaaa
    29281 tggtcagtgg aatgaatcat cagcaaatgc tttacagaga tcaggtgaac gaaggaacat
    29341 tcaacttatt agattctcat gatactgcca ggatcttgac cctttgccaa ggtaacaaag
    29401 aattgatgaa gtctgtgatg gcctttatgt ttcttcaaaa agggtctcct tgtatctatt
    29461 acgggacaga aatagggatg actggtgaag atgacccgga ttgccgcaaa tgtatgatat
    29521 gggagaagga ggaacaagat ctagagctat tcggttttat aaaagaactt gtttctctaa
    29581 ggaaacagtt gtctaagatt atttctgaag gttctactca gtggctaata gtaaatgatc
    29641 gtgaagataa gctctatttc acacggaaac tagagggaca gatcatctat gtttacttta
    29701 atcagtcaaa agaaccttgg gtagtagaac aggaaaatga ggtgattctt agtcaaaatt
    29761 gtcagttgtt agaagacggg aaagcagaga tacgacaata cggatttttg atcatgtgtt
    29821 gaaaactgtt accggtaaca acaagaaagc gtttgcaaat gaagatttgt gcccattata
    29881 ataagggtgt aacagcacgt tgaatacaaa ggaggaaatg aagatgaaaa aaagaagtgt
    29941 gaaacagttg ggtttagggt taatctcttt aggaatctta ggcggattag cggcttgtgg
    30001 caatggtaac tcttcagcag aaggggaagc cgatgaagga aaaacgttaa cagtttccgt
    30061 tgattctgga tataaagatt atataaacga aataaaaaac actttcgaaa aagaaaacga
    30121 tgtgaaaatc aagctagtag aaaaggacat gttcgatcag ttggaatcat tagcgttaga
    30181 tgggcctgct ggaaagggac ctgacgttat gatggcagca tatgaccgga tcggtgcgtt
    30241 aggacagcaa ggacatctgg cggaagtaaa actgggaaat gaagcagctt acgatgaaac
    30301 agacaaagcc caagtgactt atgatggaaa aatctacgga gaacctgcag taatcgagac
    30361 ccttgttttg tactataaca aagatttagt cgatacagca ccatcaacgt ttaaagattt
    30421 ggagaatcta tccaaagaca gtcgttttgc ttttgagtca gaagcaggaa aaaatacagg
    30481 atttttagct aaatggactg acttttatta ttcctatggt ttaattgcag gttacggtgg
    30541 ctatgtattt ggtgatgatg gcacaaatcc ttcagatatc ggtttgaata atgcaggtgc
    30601 tgtagaagga atctcttatg caacagattg gttcaaaaat gtttggccaa agggaatgca
    30661 agacataaaa agtgcaggag acttcgccag ccagcaattc atgtccaata aaactgcagc
    30721 aatcattgac ggtccatggc aagcgcaaac atacaaagaa aacaatatca attatggtgt
    30781 agccaaaatt cctactctaa ataatggaca gccttatcaa ccgtttggcg gaggaaaagg
    30841 atgggtagtc agcaattatg ctaaaaacaa agacctctct caaaaatggc ttgattatgt
    30901 aacgaatcag gaaaatcaag aaaaattctt tgaaatggta aatgaaatcc ctgcaaacca
    30961 acaagctagg gaaacagcaa aggctaaaaa cgatgaatta acaacagctg tgattgaaca
    31021 atatgaaaca gctcaagcga tgccaaacat cccagaaatg ggcgaagttt ggacaggtgc
    31081 agaaaacttg atgttcgatg cagcatcagg ttcaaaaaca ccaaaagaat ccgcagatga
    31141 agcagtaaaa acaatcagtg aagccataga acaaaaatac tgattaaaat gataaccgtc
    31201 catttttata aaaaatggaa caggtgcttc agataaaagt ggcaagagcg agatggaaaa
    31261 ttatcttgct cttgttgcca ttatcttaaa agcagtttgc ggtttagcaa gtttagaggg
    31321 ggcattcaga tgcagcaaga taaaacaagc aatatcaacg aaaaaaaaat cacgctttgt
    31381 tcacttattc cagggatggg gcagtttgta aatggccaag cttttaaagg tatcgttttt
    31441 ttcgccatat tcgtcctatt cattggtcag atgcttctgg gcggatttca tgcattggta
    31501 ggattggtta ctttgggaag tataccaatg gaagatcatt cattatttat tttgattgaa
    31561 ggcactttgc aattcatcat tacaatcatt ttcttgattt tttacggttt gaatctttta
    31621 gacgcttatc gagtagcgaa gatgcgtcaa gagggcaaaa aaatcaatcg gacgatgaag
    31681 gaagtcatac ataatatagg tgatgaaggt tttccttatc tattgacact tcccgcttat
    31741 cttctcatga tttttacgat cattttccca gtcctagtta cattgttcac tgcatttact
    31801 aattatgact tcaatcatat tccaccagcc agtttaattg attgggtagg ggctaaaaac
    31861 ttttttagta tcttcttttt aagctcatat cgtaatactt tttttgcagt cttttcgtgg
    31921 accgtgattt ggactttatg tgcaacaact ttgcaaatca cccttggtat tctcacagcg
    31981 gttgttgctc accagaaatt tattaaattc aaacgtattt tcggtatcat tttcttatta
    32041 ccatgggcgg tcccagcttt tattactatc atgagttttt cgaatatatt caacgatagc
    32101 gtaggtgcca tcaatactca agtgattcct ttcataaacc agcttccttt tgtagatatc
    32161 agtgcaatct cttggaaaac ggacccgaac tggacgaaag tagcaattat cttagtgcag
    32221 ggctggttag gatttcccta tatctatgtg atggttacag gaatcttaca atcgatttca
    32281 gaagacttat atgaagcagc aaaaatcgac ggagcaaatg catggcagcg cttttcaaac
    32341 atcacgcttc ctgctgtctt cctagtggca gcgccaacat ttattacaca gtatacgggg
    32401 aatttcaata atttctccat gatctatctg tttaacaatg gcggtcccgg aagcgtaggc
    32461 ggaggagctg gttctactga tattcttatt tcctggatct ataaacttac gactggaact
    32521 tctccgcagt attctatggc ttcagcaatc acgttgatta tctccgtcat cgttatctgt
    32581 gtgtcattgc tggtatttaa aaagacaaat gcgttcaata tgggggatta acaatgaaaa
    32641 aatttcattc gagtgctcga ttccaaaaac gactttctca cttttttact tacttttttc
    32701 ttttaatact gtcagtcatt atcatctatc cgcttttgat cacagcaagt actgccttca
    32761 agtcaggaaa tattgcagct tttagtttag atctttcaag tgactggact ttaggaaact
    32821 ttactcggct attcagtgaa acactttacg ggacttggta taaaaataca ttgatcattt
    32881 ctgtcctaac aatgatcgta caagtgactg tagtaacttt ggcgggctat acttatagtc
    32941 gctatcgttt tatcggccgc aaatcaagtt tgatgttctt tttagtgatt cagatggtac
    33001 cgactatggc agcattgact gccttttatg tcatggcttt cttattagaa gcgctcgata
    33061 agtactggtt cttgacaatg atctatatag gcggtggaat tccaatgaat acttggctga
    33121 tgaaaggata ctttgacact gtaccaatag atttagatga atctgctaaa cttgatgggg
    33181 cagggcattt caggattttt ttccagattg tattaccgct tgtcaaaccg atgctcgccg
    33241 ttcaagcatt atgggctttt atgggacctt ttggggattt catgttagct aagttcctgc
    33301 ttcgaacacc tgaaaatcag actgttgctg tcggcttaca aacatttatt tctgatgtga
    33361 caaaccaaag agtctctctt tttgctgctg gggcaatttt gattgcacta ccgatttgtc
    33421 tattattctt ctttttacaa aagaactttg ttaccggcct gacatctggc ggaacaaaag
    33481 gataggagca gagtatcatg caaacatttc cgttaaacta tttttccgca ttacgtacgc
    33541 caactcaagt gtttgcggga cgcaaactat tatcatggcc taaatttttc ttgatatttg
    33601 tatttttagt ttccttgatg gtcatgcctg tgactctttt ttatgccaat cagatccaag
    33661 cgattccatt agaacaattt ctatccgttc attcgctgat cgatgaacaa ggaacccaaa
    33721 aattttctga acttgaatta tctgaaactg ggttacaagc atcgcaacaa acgatagctg
    33781 tgacccctga aattttggta ggtgtgtctt tatctgagaa acaacaatcc gatcatggaa
    33841 cttttattga ttttgagaag gaacaatggg tgatccaaca aaaggataaa tctggtatac
    33901 gccgctatac gatgaattat agcccatctt tccaaccaga ttctgtacgt acgcccgaag
    33961 atttccaacg atttttagaa agagaatttt atgccagtaa tcgtccaaca atcattttat
    34021 cttatagttt aagtcttggt cttgtcctat ttgtcatgac gagtctcatt ttattcgggg
    34081 cttccttttt cttgtggatg acaagaaaaa gccaactttc atccattcat acattcaaag
    34141 agagtgcaaa tttgatgctg aatgttatgg gaataggttc catggcagca gcagttgtag
    34201 ggtttatcca ctttgacttt atcttgatgc taagtgtcca aacaacgata actgtcttgc
    34261 tgcttttatg ggtttttgtc aaaacaagat tcaaagataa gcgtgtcgaa tgacaagttt
    34321 aagcgattta tgcgacaatg aatgtgaaaa aaatgaaaaa taaagtagtt tttgaaaaat
    34381 ttctgaaaaa attaaacttc ttagttgatt cttttctttt ttagtattaa cgtaataggg
    34441 tagttttttt aaggaggaaa atcatgttag agaaaacttt atacaatcaa ttattttcac
    34501 gttctttctc gctccctgtc gaagtaaatt attgggacgg tacaacgaaa caatacggag
    34561 atacggacac tcctccccaa atcaaaatca atatccatga agaaatccca atgaaagaga
    34621 ttacaagtaa cgcatctttg gcactaggag aagcctatat ggatcatcga atcgagatcg
    34681 agggaagtat ccaagcattg atcaacgatg cttatcagca agcaaacagc tttttacgag
    34741 gaaaagatta tctaaaatgg ctgccaaaga aagaaaaaca tacgaaacaa aaaaacaaag
    34801 aagatatcca tgcgcattat gacttaggta acgactttta taaactttgg ctagatccaa
    34861 ctttgactta ttcttgtgct tatttcaata cacctgaaga tacgttggaa caagcacaaa
    34921 tcaataaagt ccatcatatc ttagacaaac tctttatcaa agaaggcgaa acgttgcttg
    34981 atatcggttg tggctgggga acattgatgt tcactgctac caaagagtat aatgtcaagg
    35041 caactgggat cacactaagt gaagaacaat acgatttgat taccaaaaga ataaaagaag
    35101 aacatttaga agataaatgc cgtgtattat taatggatta ccgcgaacta aaaggcgaaa
    35161 cgtttgatca tatcacaagt gtaggtatgt tcgaacatgt aggttctgaa aatcttgaag
    35221 gttatttcaa agtggtaaaa gatttattga aaccaaaagg aacagctttg atccatggaa
    35281 tcagccgaca acaaggcggt gcgacaaatg cttggatcaa taaatatatt ttccctggcg
    35341 gttatgtccc tggtgttact gaattagttg ataatattac aaaaaacgat ttgcaactga
    35401 ttgatttgga aagcttacgt cgtgactacc aattgacatt agaacattgg acaaagaatt
    35461 ttcatcaagt ccaagatcaa gtcacgaaag aaatgggtga ttccttctat cgaatgtggg
    35521 atctatattt acaagcttgt gcagcttcat ttgaatcaag taatatcgat gtgattcaat
    35581 atttgcttgt tcaacctgga aacaatgaca tcccaatgca tcgtgcataa ttgaggcaaa
    35641 agtactgttt aaagactatt taaaaagaag ctaagtttta tgatgctcca gaagtgaact
    35701 tttttgtcta acttctagga ttgtctctaa agcttagttt ctttttcttt atattttcat
    35761 aataaaagtt tggttcttta atttttaaac atgacagaaa attttttctt cgttatactt
    35821 gtaatagaat actattttat aacaaagggg atcgaatcaa tgaaaaaaac tgttatttat
    35881 actagtttat ttgcagcttt gtttttagct gcttgtcaat caaataccaa tcaaggcaca
    35941 tctgggaata cgtcttccag tagcgaacag cgctcttcac aggtatcgga ctcctcaaca
    36001 aaagcaagca catcttcgtc tagccagaca aaaagttcta gtgaagcaag tactcagtca
    36061 gatatcaaag tgtctgcagc tcgtgcgatc gaattgttcc aagataaata cccagatact
    36121 gcaattacta gtttggaatt agatagtgat tggggatctt acttctacaa gatagaaggt
    36181 gtcgatgatc aaaaagaata cgaagcaaga attgatgctc aaaatgaaaa aatggagaca
    36241 gagaatccag aaactctaga tcgagatgaa caaaatggtc aaaagaaaaa agaagatggt
    36301 ctggatgttg ataaattgat cacgattgag gaagcagcaa aaatcgccgt cgacaaagta
    36361 ggaagcggta cggcaacaga ttgggatttg gataaagatt taggtactac ttattgggaa
    36421 gtcaaagtaa aagacggcaa tcaaagtaca tcagtcaaga tcaatagcca aacaggcgaa
    36481 gtactgtcaa ctgaacaaga tgattaagaa tttcttcgga aaacaagagt tgatttttct
    36541 tttagtcatg atacactaga caaaataact tgtttgaagg agcgatttac ttgagtttag
    36601 aaacgacctt tgccaatcat atctttgcca atcctttgat gaatgcatct ggtgtccact
    36661 gcatgactac ccaggaatta gatgaactgg ctcattcaga agcaggagca tttatcacta
    36721 agagctgtac gatcaatgaa cgaaaaggaa atcccgaacc acgctatttt gatgttcctt
    36781 tagggagtat caattctatg ggacttccaa acttaggttt ttcttattat ctagagtatg
    36841 ctcttgcgta tgaaaaagtg caggaaaacc aaaatcaacc gttatttttt tccattgccg
    36901 ggatgagtgt ccaggaaaac ttggaaatgc ttgagaaaat agaaaaaagt gggtttaatg
    36961 ggatcactga attaaattta tcttgtccca atgtgcctgg aaaaccacaa ctagcttacg
    37021 attttgaggc cacttatgaa acactgaaag aagtattcag catcttttct aagccgctag
    37081 gaatcaaatt gcctccttat tttgattttg ctcattttga tcagatggct gatatcttga
    37141 atcagtttcc attgacctat gtcaatgcta tcaacagtgt gggcaatggt ttgtatatcg
    37201 atacagaaca agaagcagta gtcatcaaac caaaagaagg gttcggcggc attggcggag
    37261 agtatatcaa gccgactgct ttggcgaatg ttcgcgcttt ttatacacgt ttgaaaccag
    37321 aaatccaaat cattggtaca ggtgggattc ggacaggaca agatgcattt gaacatctgc
    37381 tatgtggcgc aagcatgtta cagattggta cagagcttca taaagaaggg ccggaaattt
    37441 tttctagaat cataaaagaa ttaacgcaga ttatgtcaga aaaaggatat acgtcaattg
    37501 acgaatttaa aggcaaatta cgcaccattt catagcaaag agacgaaggt catgattgat
    37561 cttcgtcttt ttttataaaa aacactcgtc tgttcattcc tgtgcaagcg tactcattgt
    37621 atgttagacg aacttgtggg aaaataacag aggcaaaaag gaggataata tgaatgatgc
    37681 aacagaatta gcaaaagagc ttaaagaaaa agaatttagt tttcaagaac tagttacgga
    37741 tatacagaaa aaaataaaga agcaaaatca tgaattgaat gcgtttgtcg cgcttgaacc
    37801 attcgagagt aattcccacg agctattgat tccccagtca gaaaaagggc cattggctgg
    37861 tatcccattt cctttaaaaa tgctcggtca agaaaaaaag ggatggctag ctacttcagg
    37921 atctcgttta tttgaaacac accgagcttc tcatacatct aattatgtgc ggcaagcaga
    37981 agcgataggt ctagttcctt ttggacaaac caatgcgcca gagtttggtt tcaaaaatat
    38041 tacagaccca gtcatctatg gcccagcaag aaacccttgg aaccttgaat gtacaccggg
    38101 cggatcaagt ggaggagctg ctgctgcagt ttcatcagga atcgttcctt tagctggtgc
    38161 tagtgatggc ggtggttcta ttagaatacc cgcttcgttt tgcggattga ttgggctgaa
    38221 accttcgcga gggacgatgc cagttggtcc atatgcttgg agaggatggc aaggagctgc
    38281 tattgatttc ggcttgacag tttctatgag agacacggag gctttatttt atgggatgag
    38341 aagtatccat tccggtgcgc catatcaagt atcgccagtt gagtggcaaa cacatccaag
    38401 aaaaaaacgg ttgaagatag ctttttgtgt tgattcaccg atccactcac agattagtga
    38461 agaggcagta cttgctgtaa aaaaagctgt agatttcctt ggaaaagaag ggcatgattt
    38521 gacagagatt ccttacccac tagatggtcg taaactgatc acgtcttatt atcagatgaa
    38581 tggcgcagaa acagcagcaa tgatccgctc gattgaaaca ggcatccagc gagctgtcag
    38641 aaaagaagaa cttgaaccca tttcttggac tttgttccaa tatggtcaga agctctctgc
    38701 cgctacttac atcgattctc tgcatgtatg ggatgaagca gcaattgtca tggaaaacct
    38761 acatcaagaa tacgatctat tcctttcacc aacgactgca tttaccgccc ctaaaatcag
    38821 cgaagatttg caaagtgatg cgataagagc acagatggaa cacatcagtg attgttctga
    38881 agtagaaggt ttagacgttg tttcttccat gtttgaggag agcttgaaga tcacacctta
    38941 tacacagttg gctaatctga ctggacaacc agcaatcagc ttgcctactc atttgacaaa
    39001 agaaaacatg cctctaggaa tccaatttat ggctgccaga ggaagagaag atttgctatt
    39061 tcaaatcggc tatctatttg aagcaaatca tcagttccat ttaccaaatc catggaaata
    39121 gcagaaacaa ggagaagaag aatgaagaaa aaaagattgt ttgctctaat aactagtgcc
    39181 ttttttattt taagcgcttg cggtacagct gccgctaatc aggaagataa cagctggagt
    39241 aatgtagaaa aaaagaaaga attagtagta gcgacttctg gcacactgtt tcccgcatct
    39301 tattataatg atcaaaatga attagttggt tatgatgtag atttggcaaa agaggtggct
    39361 aaacggttaa atgtcaaaat ctcctttaaa gaatacaacg tggatggaca aatcacttct
    39421 gtttcaaaaa gagaatcaga ttttgctgcg aacgattttg gtatcacaaa agaaagagaa
    39481 aaaaaattct ccctttcaga gactttcaaa tattcatttg acagtatgat cgtacgaaaa
    39541 aaagatgatt caggaatcca ctcacttgaa gatcttaaag ggaaaaaagc agctggcgaa
    39601 cccaatacta gctatatgcg tttagcggaa aaacacggag ctaaattagt cacatatgac
    39661 aatgccacga atgatcagta tttaagcgat gtagcaaatg gtcgaacaga tgttattcta
    39721 aatgattatt atctgcagaa aatggccgta tctgctttac cagatatccc tgtaaaaata
    39781 ctggagaacg tttatttcaa tgaaaacgaa agtggttttt tattgaaaga aggaaatgat
    39841 gctctgaaaa agaaaatcga tggcgtatta aaagagatga aagaagatgg gacgacaaag
    39901 aaaatatctg aaaaatattt tcaaactgat gtttcagtca aaccgaaaga aacgatggct
    39961 gagtcggtga gtgagtgatg tactttcctc taattgattt ttccctcatg tggacttcac
    40021 ttcctttcgt actgcaaggc ttggtataca cattagggat tggcttcgtg tcgtttcttt
    40081 taggaaattt ggtagggttg cttttgacag ttttaggtct tttggattgg ctgcctctga
    40141 atgtatttat tcgtttttat ttatcgttct ttagaggcat tcccgcacta gtcctgctgt
    40201 ttctgttata tttcgggtta ccttatcaat taagtgcctt aacggcttca gtcatctgct
    40261 ttacgattac cagcagtgca tttattggtg aaatctatcg aggatcacta gcaggcgttt
    40321 cttcaggtca gtgggacgct ggtttagctt ctggtttttc tttctttggg gtattgaaat
    40381 atatcgtttt gccgcaagct tttcgaatct cagtaccggc actcagtaac gtagctatgg
    40441 atctagtgaa aggtacttcg ttagcagcaa caatcacagt gccagaaata tttcaaaaag
    40501 caaagatcat aggcggtcgg acattcgatt atttttctct ctatatttta gttgcactga
    40561 tttattgggt catttgtatc gggatcgaaa aagtgcaaca gtatttagaa aaacgttcta
    40621 tccatcagtc aatctgaaaa atagaaatgg tgctagcgtg gctggcacca tttctatttt
    40681 ttgtttcttg tatattctgc ctagtatttt aaaaccattt catgtaaaat gtaaatcgaa
    40741 aatatgttcg gaggtagaaa acatgaagac cccgctaacc atcgaaatag aatcctcgtt
    40801 ataccaatat tgcatcgagc aaggcggctt ggttgtagaa gaagtcacga tgcctgatga
    40861 ccaaggaatt gtagatacgc ttagctgctt gacaaaattt gatggaagcc gagaatggcg
    40921 atgctatgag ctgaaagtga caaaagcaga ttttcgctct acagccaaac tgtcatttgt
    40981 tggacattat aattattttg ttttaccaag taaactatat gaagaagtaa aaactgaaat
    41041 ccctacagga ataggtgttt tagtgtatcg tccgtacacg gttgaagagg agatgcctgc
    41101 ctctggtaca tttctgattg cgaaaaaagc agcaaagaaa gagcttgctg taccggaaga
    41161 agctttgaca aaccgcttca tggcttcttt attcagggaa gtaagaaaag caaagcaaat
    41221 ggagcgaggc gtaaaatatt ttccaaaaga tcaattgtac aaagaattga aaagacgttc
    41281 agaaaatcaa gatccatttt ctaacgaacg atattatgaa cggtttttag aggaaatagc
    41341 tagtcaggca atcaatgagt tgcaagaaga agttatcgcg cttcagcatg attatgaata
    41401 tttgaggcaa caacagcaga tccgtcggtt gcctacagag ccattggagt gaaaaatatg
    41461 tactatcctt atttaagagg aaaacaattt gacctattag cattaaaaga agcactgtcg
    41521 cgaggattgc tctccaataa aatccaacca gtgatcgaac cagtacgtga ttccgctacg
    41581 cttaaaaatg tgatcgaatt atttcaaaaa aagcaccatc caatcgctgt gatccaaaat
    41641 ccccaagtag gacaattcaa attattcgat cagcatgtgc acagttggac agtcaaagaa
    41701 aatagctctg tcgtccctgc acagatctta acgccagaaa atctcagtga agtactggat
    41761 tcacctccag cgtttcttgt tttcgatgga cagcactacc caaaagatac agaggtgtgg
    41821 aaacagttgg caggagttga ttcgaaattt ttgataccag atactagtcg ctttcgcatc
    41881 tggctgcccg aaaacaaaat tgtgatccgt gactcttttc aaacgagaaa gcatgttgaa
    41941 agttatgcag ataaaacaga tgactttttt agtgatgact atcttttttt ccacgcagac
    42001 ggatatattg gattttctga tttcacgatc gaaggtagcc gttatttcga taaagggttc
    42061 cctagccgag cgcttgctat ccatctaacg tatattgatg cttacggcaa tatacgcgtg
    42121 aaacattttg tctctgacag taacgactct gctaaagacc aagcattgaa gtttcttgaa
    42181 gcaggagata aaatgaaaaa atggatcatg cgtcaccatc atcaactctt gattacttca
    42241 ggaatgattg aattattggc tctttaccag caacaaaaat tccctggact gggagtgttg
    42301 aaaaagtggt cacttatgca tcatttggaa cttattagtc acctgctaga tcatccaaaa
    42361 gattggctta aatattattc aaaagttcag gaactatatg aagtgatcca atgattagat
    42421 aatgtaaatg aaaaaggaat agaaaagagg aaactggatg tatattcggc aaacaaggaa
    42481 agaagaagta gaagaagtgg tagaaatcat taatgccgca gcggatttca ttgcttcgca
    42541 aggctctcct caatggcaaa acgggcagaa accaacaaaa gaaacgattt tgaacgatat
    42601 tgaaagaaaa gaaagctatg tattaatagt ggaagatcaa attgctggaa cagctgcatt
    42661 agtctcaagc gtggatcccg tttacacaaa aatagatggt agttggaaag aatcaactgg
    42721 tccttattta tcgattcatc gtgtcgcttt agcagaaagt actcgaggaa aaaaactagc
    42781 aaaaccgttc ttagaacatt tgttgacgat tgcagccagc aaaaatatcc aagatatccg
    42841 tatcgatact tatccagcta atgaaccgat ggaaaagacc atctattctg ctggattcac
    42901 ttatcaaggg atgattgaat ttccttttga acacggcgaa agaaaagcat atcaaataat
    42961 catcaattga ctataaaatc atctgagcaa agataaagac attaacacta ctgaaaaaaa
    43021 caatcatatg atagccaaga ggttgtaaca gaagtggcca gcttcaaaaa acaagaaaga
    43081 atttccgaaa atgcttctca aatttttgtg gaattttagc gtattttacg aaatagctgc
    43141 tttctgaaac tcccttcatc gagaggtcgt gacatagctt ttgtcacgac ctctcggctt
    43201 atcctacgag tattattcga tacatttata tcaataatcc tttttttatt ttatctgcag
    43261 cttcttggaa cgctccatcg tttgacacga ctaaaagtga ggggatagct ttcaaacgtt
    43321 tcaactgtgc taaatcttct acataaaccg ccagtctggc tgaaatctgt tcagatgaat
    43381 ctccacgttt tttcaggcgc gattgcaatg tttctttaga gatcgtaata aagataggac
    43441 ggactaccgt cccgaaatac tcatataaat gccaaaaacc atttgctgtc aaaacggcgt
    43501 aacaatttcc tactgtcagt ttctttttaa tttctgcttt actggaacca tatttatgtt
    43561 gagcatattg gtcccactct gcaaactcat ttcccttgat catctgattg aatgtattgt
    43621 cagaaacaaa ataataatcc tcttgatctt tttccccagg tcttggtgat cgattcgtgt
    43681 aagtaatgat cttttgttcc ggctgaaaaa tagcttctgc gagtgaagtt tttccagaac
    43741 cagaaggacc gataaataca tagcaataat tcattgtcat ttctcctttg atgattgaac
    43801 aagtgaaaaa gctttggaaa atatttccaa agcttttaaa agtaactttg cttttttgta
    43861 tattatacca gcaagccagt cacaatagca cttaaaaagc tgactaaagt cgctgttagc
    43921 aaaattttca atccgtgttt tgcgacaaga tttccttttt cttcatttaa tcccttcatc
    43981 gcaccagaaa tgatcccgat agaagagaag ttggcaaatg aaacgaggaa gacagatata
    44041 attgctgttg tacgctcagt aaaatggaaa ttaccatttg ctaattctgt catagcaaca
    44101 aattcatttg taaccagttt agtcgccatg atactaccgg catccactgc ttctttccat
    44161 gggattccgc tgatgaaggc aagaggggca aagatgtaac ctaggatctc ttggaaagtg
    44221 atgccgaata tcccgtggaa gatagcattg atcatggcaa tcaaagcaac aaaaccgata
    44281 agcatagcag caaccgtgat cgccacatgg aaaccatcaa gaatgtattc tcccaacatt
    44341 tggaaaaatg tctgttcttt gttttcttca atgatcagct catcttcttt ttcttctaat
    44401 acatagggat ttacaattga agccagaata taaccgccaa ataagttcaa aacaagggca
    44461 gtgatcacat atttagaatc gatcagtgcc atataagatc cgacgatcga catagaaact
    44521 gtagacatag ccgatacact catggtgaac aaacgtttct cagataggaa gggtaattcc
    44581 ttttttatgg aaataaaaac ttccgattgt cctaaaatag cagaagctac tccattataa
    44641 gattccaatt tgcccatccc gttgatttta cttaatccta gaccaacagc tttcataaat
    44701 aatggaagaa tcttgatata gcgtagaata ccgataatag cagaaataaa tacaattggc
    44761 atcaatacgg aaagaaagaa aggtgcactt cctttatttg ctacaccggc aaaaacaaag
    44821 tcaacacctt gcccagcaaa agcaagaaga tgatcaaaca tttttgccaa tacagaaaca
    44881 accgctgtac caaaagttgt acgtaataat acgaaaccaa gaacaagctg taaaacgaac
    44941 atcacaatta gtggtttcaa tttgattgct tttttgttac tgctgatcaa aaaagcaagc
    45001 cctaatgtga caaataaacc aataatacct aataaataac tcaaataatc actcctattc
    45061 caaattccgt tttattttac ctgttttgca aataaaatac taatctttag tgaaattttc
    45121 attacttttt tagagtgaaa cgcattcatt ttccattaga aaataaagcg aacaaaataa
    45181 aaggtttaaa aagtgtaacg tcttagtaaa gtctataaaa aaacgaaact atcgattaag
    45241 ctaatagttt cgttttcaac atttataa
//
LOCUS       DACDYI010000014        43617 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.014, whole genome shotgun sequence.
ACCESSION   DACDYI010000014 DACDYI010000000
VERSION     DACDYI010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.014"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            99..746
                     /locus_tag="IWM52_000748"
     CDS             99..746
                     /locus_tag="IWM52_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP9973021.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            1139..3784
                     /locus_tag="IWM52_000749"
     CDS             1139..3784
                     /locus_tag="IWM52_000749"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAP9973022.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="IWM52_000750"
     CDS             4094..5407
                     /locus_tag="IWM52_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAP9973023.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="IWM52_000751"
     CDS             5397..6050
                     /locus_tag="IWM52_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9973024.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6776
                     /gene="radC"
                     /locus_tag="IWM52_000752"
     CDS             6105..6776
                     /gene="radC"
                     /locus_tag="IWM52_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HAP9973025.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGIYKLVHINYIRKFTVILPSGNTIMLSIIERHNAFFSS
                     SEDSL"
     gene            complement(6671..8023)
                     /locus_tag="IWM52_000753"
     CDS             complement(6671..8023)
                     /locus_tag="IWM52_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9973026.1"
                     /translation="MKRVALYMRVSTEQQAKHGDSLREQKETLYEYIEQHKDLKVVNE
                     YVDGGISGQKINRDEFQKLLQDVKENKIDLILFTKLDRWFRNLRHYLNTQEILEKHNV
                     SWNAVSQQYYDTTTAYGRTFIAQVMSFAELEAQIDSERIKAVMANKIAQGEVVSGKTP
                     LGYSIENKKLVINDDAPIVIDIFNYFLSSGSLRKTVYYLGSQYGIVRDYQSVKNMLTN
                     KKYIGELRNNKNYCPPIIDKKLFYAVQKALPKNLKTNAKRDYIFKGLLKCSDCQGSVA
                     GQTIKARYKKKDGTESIYERTCYRCVKRRNNKLRCTNKRAFYEKNLERYIFEATKQKF
                     EQIQINYSKKQPKIIKKKNSKKNIENKLDRLKKAYLNEVIDLEEYKKDREALMKELNE
                     IEVEPAKIDIKNVEFILSKEFDEIYKESSEEEKNALWRSIIDNIIVFPDGNITVNFLI
                     "
     gene            complement(8089..9378)
                     /locus_tag="IWM52_000754"
     CDS             complement(8089..9378)
                     /locus_tag="IWM52_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAP9973027.1"
                     /translation="MGVLLFLVGIVLIIMGSSKLSEAKKLKDKTSALESKVKEYSQEL
                     NARETFISEKEKNISKLNKNLSTLENKIDILNAESQRLDDEINQKYDTSLVTIYKSVI
                     IPNIDETSSSEIKNKLSLLKIKEKDFILDGAINIPSYLNNREANNIKKKMLIPLESEI
                     TGLLNKLTISNVDSTREKIIRTFDKINKLFKNDEAEFKKELLELKLQELELNYSYIVK
                     VNDEKEQQKAIKEQMIEEEKVRREIEREKKRIDKEERQFNSEITKLMAYMQKASADVE
                     KELYADKIKELEEKLRELETVKEDVLQRELNTRAGYVYVISNIGSFGEDVYKIGMTRR
                     LEPMDRIKELSSASVPFEFDVHAMIFSEDAPTLENQLHSHFRKNEVNKVNQRKEFFKV
                     SLDEIEKVVLENYNGTVSFTKLAQAEQYRRSLELTDV"
     gene            complement(9465..10169)
                     /locus_tag="IWM52_000755"
     CDS             complement(9465..10169)
                     /locus_tag="IWM52_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HAP9973028.1"
                     /translation="MFAENLKFLREKYNIDQQELAEKLGRKSSSSISEWEKGKYTPKL
                     KTLNEIAKIFNVDIDDLMNIDLSISNQIYNILPIYNQLNDENKVATYEFASNRLEEQK
                     QENVLDFPKKEKIPTVHNSASAANPTELTYGDTVVEEEEFERVPSAADFAVPIIGDSM
                     EPVIRNGQFVFVKEQPDVEDGEIAIVELGGDGVTCKEIYKDYENQTIILRSINDLYED
                     RIVSPEQIRIIGKVVF"
     gene            10369..10635
                     /locus_tag="IWM52_000756"
     CDS             10369..10635
                     /locus_tag="IWM52_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9973029.1"
                     /translation="MDTAKKEKYTLRQLRALKGYSKEELSRKSKVTSRTIFIYENDVD
                     KMRNGKYATLEKIAKALGVRVSDIFLDPDSEKPKSITGETKQVS"
     gene            complement(10803..11033)
                     /locus_tag="IWM52_000757"
     CDS             complement(10803..11033)
                     /locus_tag="IWM52_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP9973030.1"
                     /translation="MGKNQWVSPRNGKWAVRGEGNSEDTKLFDNKSDAVEYGKKISKK
                     QQSEFIVQKKNGQIQSKDSFGNDPIPPRDKEH"
     gene            11105..11260
                     /locus_tag="IWM52_000758"
     CDS             11105..11260
                     /locus_tag="IWM52_000758"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973031.1"
                     /translation="MSNKPKITLNGTPNLQRLADEMARIRSRHWGVTVVATIIEQKEK
                     PTQPASK"
     gene            complement(11569..11793)
                     /locus_tag="IWM52_000759"
     CDS             complement(11569..11793)
                     /locus_tag="IWM52_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004401476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP9973032.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            12060..12395
                     /locus_tag="IWM52_000760"
     CDS             12060..12395
                     /locus_tag="IWM52_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973033.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            12628..13569
                     /locus_tag="IWM52_000761"
     CDS             12628..13569
                     /locus_tag="IWM52_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAP9973034.1"
                     /translation="MSKSTLEMSHQEWLEDRKRGIGGSDVATVLGLNKYKSPYQLWLE
                     KTGQIELKDLESEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPVIDGSGATADFLKEKYADVEENQTA
                     LPSRFDELIEQKRELKRTKKEIESSIRQVDNEIISELGKREASIGITQRNIISWKLVR
                     TRRMNTKKLAEKYPDVANDEEIYNVTESRRLTEKEIK"
     gene            13571..14461
                     /locus_tag="IWM52_000762"
     CDS             13571..14461
                     /locus_tag="IWM52_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HAP9973035.1"
                     /translation="MATNESLKNQLAEKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            14504..15304
                     /locus_tag="IWM52_000763"
     CDS             14504..15304
                     /locus_tag="IWM52_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP9973036.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWSLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            15319..16170
                     /locus_tag="IWM52_000764"
     CDS             15319..16170
                     /locus_tag="IWM52_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9973037.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            16167..16484
                     /locus_tag="IWM52_000765"
     CDS             16167..16484
                     /locus_tag="IWM52_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973038.1"
                     /translation="MNLTKQEKRICRQIMLQIIGDRDMDELSQQESRMVRKISKLIGA
                     DTMDRSKPLPKLNFESFTFEKYSHLREVGYSVNAIRKALGVGSSVWAKWRKENVNEVN
                     QND"
     gene            16477..16659
                     /locus_tag="IWM52_000766"
     CDS             16477..16659
                     /locus_tag="IWM52_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973039.1"
                     /translation="MIKLIGKETLKVYYVAETKAEVSRWLLKMFNKTRANASVSQKAS
                     VSETGMPEAMWISRRN"
     gene            16661..17131
                     /locus_tag="IWM52_000767"
     CDS             16661..17131
                     /locus_tag="IWM52_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973040.1"
                     /translation="MAMIPKFRAWDREKDRMIYPSTTGVCFEMNDDGISVLDIDVDYP
                     NDYGFPKIASILMQSTTLKDKNGVEIFEGDIALITHKSTNYADTYWHSYVEVFRAKNG
                     AYRIRGEHIYETELYSNRKQLTVAGNVYNSPELLKRDRFMTTTENPELLKEEQR"
     gene            17128..17670
                     /locus_tag="IWM52_000768"
     CDS             17128..17670
                     /locus_tag="IWM52_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP9973041.1"
                     /translation="MNKQELIDKYTAEISRLRPYCPNRHLISDQLKYGLYKEILEDLK
                     QLDEPQKPAVPKFVAEWIEYAKKKGDSLAISFKPWNLYGVEYSKTDRWIEDNQGTFAR
                     AWLIGYEVEKEPLYYVKLPVVYFNHWDLETYLMKDDRGNITIADNNDFDDMKFTESEI
                     KAIDERYWPFAVPVEEVAEG"
     gene            17667..18050
                     /locus_tag="IWM52_000769"
     CDS             17667..18050
                     /locus_tag="IWM52_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973042.1"
                     /translation="MSNLKVGDIVFYKGDEGIIREVMTDGKENFYRVDVSRKSILYLY
                     EDELDLGTQKLNDNQKMVFDYLKSNVEQGGKSVMYSIFLLGDWDSRIGAARNVDIAYC
                     GLNGKQEAEVLSAFAQWGLEQEEAE"
     gene            18047..18277
                     /locus_tag="IWM52_000770"
     CDS             18047..18277
                     /locus_tag="IWM52_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973043.1"
                     /translation="MKYEIPLSEADVQSIINGREVNKKLPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSIDDTFRLGS"
     gene            18284..18487
                     /locus_tag="IWM52_000771"
     CDS             18284..18487
                     /locus_tag="IWM52_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HAP9973044.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASNAIRKFGISILNDDRKWEEMR"
     gene            18484..18780
                     /locus_tag="IWM52_000772"
     CDS             18484..18780
                     /locus_tag="IWM52_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973045.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWTAKSKENLAVVRLVDIELINDK"
     gene            18857..19270
                     /locus_tag="IWM52_000773"
     CDS             18857..19270
                     /locus_tag="IWM52_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP9973046.1"
                     /translation="MQLLREVAFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            19283..19669
                     /locus_tag="IWM52_000774"
     CDS             19283..19669
                     /locus_tag="IWM52_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973047.1"
                     /translation="MWKDYVSLKELKKDLIFKKIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHSSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKEKLCVVTDA"
     gene            20161..20233
                     /locus_tag="IWM52_000775"
     tRNA            20161..20233
                     /locus_tag="IWM52_000775"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20194..20196,aa:Lys,seq:ttt)
     gene            20619..20879
                     /locus_tag="IWM52_000776"
     CDS             20619..20879
                     /locus_tag="IWM52_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973048.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSVFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            21484..22317
                     /locus_tag="IWM52_000777"
     CDS             21484..22317
                     /locus_tag="IWM52_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small subunit of terminase"
                     /protein_id="HAP9973049.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELKSNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFETLSDEEKDIYSSLNDNPSFVLSEEIRLLKIRQFRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKNPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            22310..23725
                     /locus_tag="IWM52_000778"
     CDS             22310..23725
                     /locus_tag="IWM52_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase B"
                     /protein_id="HAP9973050.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPENMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTTVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            23737..25266
                     /locus_tag="IWM52_000779"
     CDS             23737..25266
                     /locus_tag="IWM52_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP9973051.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKDPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            25358..26236
                     /locus_tag="IWM52_000780"
     CDS             25358..26236
                     /locus_tag="IWM52_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAP9973052.1"
                     /translation="MMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            26238..26555
                     /locus_tag="IWM52_000781"
     CDS             26238..26555
                     /locus_tag="IWM52_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973053.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            26604..27305
                     /locus_tag="IWM52_000782"
     CDS             26604..27305
                     /locus_tag="IWM52_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HAP9973054.1"
                     /translation="MVEWWGATIKTQSNAGRENESWGERRMSMKHKSLMPMNLQYFAE
                     GDDQKFSFDDFKSFVESNEEAQKFIQSQSQSVADKQLEAWKQNNLDKIKQDTIKEYEE
                     SKKNKSPEQIQLEKLQAEFEAEKALRVTSDNKAFVAEQIAGLELDGELKESISQFMLN
                     NLVSSDTDFTKNAVEGFTSVLNAINEKHADALKELQMKSAFGGTQQSNNQVQQNNETL
                     TNPEEQLGQILQQFN"
     gene            27320..28210
                     /locus_tag="IWM52_000783"
     CDS             27320..28210
                     /locus_tag="IWM52_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HAP9973055.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLFGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            28233..28448
                     /locus_tag="IWM52_000784"
     CDS             28233..28448
                     /locus_tag="IWM52_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973056.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            28460..28801
                     /locus_tag="IWM52_000785"
     CDS             28460..28801
                     /locus_tag="IWM52_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973057.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            28801..29172
                     /locus_tag="IWM52_000786"
     CDS             28801..29172
                     /locus_tag="IWM52_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973058.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            29165..29560
                     /locus_tag="IWM52_000787"
     CDS             29165..29560
                     /locus_tag="IWM52_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAP9973059.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            29562..29939
                     /locus_tag="IWM52_000788"
     CDS             29562..29939
                     /locus_tag="IWM52_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973060.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            29940..30548
                     /locus_tag="IWM52_000789"
     CDS             29940..30548
                     /locus_tag="IWM52_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP9973061.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            30548..30889
                     /locus_tag="IWM52_000790"
     CDS             30548..30889
                     /locus_tag="IWM52_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973062.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            31131..33920
                     /locus_tag="IWM52_000791"
     CDS             31131..33920
                     /locus_tag="IWM52_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HAP9973063.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFIFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANSFVLIIGAVVGAI
                     AVFGYFMATNEEFRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNEIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWID
                     LKYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVN
                     SAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTI
                     KGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTP
                     SVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQ
                     MKRDAGAVGGTLYAT"
     gene            33910..34650
                     /locus_tag="IWM52_000792"
     CDS             33910..34650
                     /locus_tag="IWM52_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP9973064.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYRNVTRKIECYYKAPSIDVVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            34647..37409
                     /locus_tag="IWM52_000793"
     CDS             34647..37409
                     /locus_tag="IWM52_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP9973065.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLAEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRLIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     SLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFTSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSAGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWRIYDILCNNGFTK
                     QSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLF
                     NAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQAAYAFERNYE
                     RPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYS
                     GYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIIN
                     WARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISS
                     IVIPPK"
     gene            37422..38330
                     /locus_tag="IWM52_000794"
     CDS             37422..38330
                     /locus_tag="IWM52_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HAP9973066.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            38330..38950
                     /locus_tag="IWM52_000795"
     CDS             38330..38950
                     /locus_tag="IWM52_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973067.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            38954..39403
                     /locus_tag="IWM52_000796"
     CDS             38954..39403
                     /locus_tag="IWM52_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973068.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            39403..39897
                     /locus_tag="IWM52_000797"
     CDS             39403..39897
                     /locus_tag="IWM52_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973069.1"
                     /translation="MADIVQLKEDGVAKYLKTHVKAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            39911..40258
                     /locus_tag="IWM52_000798"
     CDS             39911..40258
                     /locus_tag="IWM52_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973070.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKSGTAVDDIEDVDQLKQLNALLTKQLAISVQEQEKMQQMLAQLTM
                     EVASIKNGGKSNE"
     gene            40251..40391
                     /locus_tag="IWM52_000799"
     CDS             40251..40391
                     /locus_tag="IWM52_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP9973071.1"
                     /translation="MNSFPGFENIKQFYDWGCYTDQDLLDYVNMNCLTKDQYKQITGN
                     EI"
     gene            40491..40880
                     /locus_tag="IWM52_000800"
     CDS             40491..40880
                     /locus_tag="IWM52_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP9973072.1"
                     /translation="MTGVIGGTIVGLLGGMDNILHVLIFLVGVDFLTGLAKAWKLKEI
                     SSEIGFEGLLKKVLIFVVIAVAVDVQKIVGNSIPLREIVIMFYVANEGISFLENISVF
                     LPLPDKLKEVFQQIRNDTENKDRGGTK"
     gene            40877..41887
                     /locus_tag="IWM52_000801"
     CDS             40877..41887
                     /locus_tag="IWM52_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9973073.1"
                     /translation="MKKKIFVGAIIALFLLPINAFAYSINNEFNLGANEGSSQVANNQ
                     YILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSYAN
                     ANSPVQIELQHTHDKVTFEKNYKAYVELARDSANKYGIPLTLDTPYNQPGIKSHLWVT
                     QNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTL
                     SDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPD
                     VNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIP
                     KR"
     gene            42002..43210
                     /locus_tag="IWM52_000802"
     CDS             42002..43210
                     /locus_tag="IWM52_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973074.1"
                     /translation="MTAEIGIMNKSGIVLASDSASTIGDSKVYNTAKKLFTLDSMHSV
                     GIMIYGNAEFNGIPWEIIITQYKKSIGSSVFNTLEEYADNFIEFVKTASFIRSEQTEQ
                     EQMIGVFQKIISGLFESIEVDINFLISQGTQIDKDVLVKLLQTKMNTNLSQQSQTFIL
                     DIEKALFLSNYGEILKDILNSISTMEGVSEAISEEIQSYVYEIIIRDDVYSSPTGIVI
                     AGYGRMDIFPKLYSYNMFGFVMNILKYSEYESAQIGNDNGSLRSTILPFAQSDVVNTV
                     VQGVDPQITNYLSSQVDSFDDNGKNTYINIIKNISEFQQNQFIFPLLNMIALLPVEET
                     AIIAETLLNLTSFKRKYTTSVETVGGPIDVLAITPNDGPIWIKRKHYFDIDNNIGYRL
                     RKERANDYNN"
BASE COUNT        15286 a         6543 c         8937 g        12851 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccctatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttccggaata tataagttag
     6661 tacacataaa ttatataagg aaatttactg ttatattgcc atctggaaat actattatgt
     6721 tatcgataat tgaacgccat aatgcatttt tttcttcttc cgaagattct ttataaattt
     6781 catcgaattc ttttgataga ataaattcga cattttttat atctatttta gctggctcta
     6841 cttcaatttc atttaattct ttcattaaag cttctcgatc ttttttgtat tcctctaagt
     6901 ctataacttc attcaagtag gctttcttca atctatctaa tttattctca atatttttct
     6961 tactattttt ctttttaata atctttggtt gtttttttga atagtttatt tgtatttgtt
     7021 caaatttttg tttagtagct tcaaaaatat aacgttctaa atttttttcg taaaaagcgc
     7081 gcttatttgt gcaacgtaat ttattatttc ttcgttttac acatctataa caagtccttt
     7141 cgtatataga ttctgtaccg tcttttttct tatatcttgc tttaatagtt tgaccagcta
     7201 ctgatccctg acagtcagaa cattttaata aacctttaaa aatgtaatca cgttttgcat
     7261 ttgtttttaa atttttaggt aaggcttttt gaacagcata aaaaagtttt ttatcaataa
     7321 taggaggaca gtaattttta ttatttctta attcaccaat gtattttttg ttcgtcaaca
     7381 tatttttaac actttgataa tctctaacga ttccatactg agagcctaaa taataaacag
     7441 tttttcttaa actaccacta gaaagaaagt agttgaatat atctatcacg ataggagcat
     7501 catcatttat tactaatttc ttattttcta tagagtagcc taacggagtt ttcccgctta
     7561 ctacttcacc ttgtgcaatt ttatttgcca taacagcttt gatgcgttct gaatcaattt
     7621 gggcttccaa ttcagcaaaa ctcattactt gtgcaatgaa agttctgcca tacgctgtag
     7681 ttgtatcata atattgttga gaaacagcat tccaagaaac attgtgtttt tctaaaattt
     7741 cttgagtgtt taaataatgt cgtagatttc taaaccatct atctaatttt gtaaataaaa
     7801 ttagatctat tttattttct tttacatctt gtaatagttt ttgaaattca tcacgattta
     7861 ttttttgacc agaaatacca ccgtctacat attcattaac tactttcaaa tccttgtgtt
     7921 gttcgatata ttcatagagg gtttcttttt gttctctaag actatctcca tgtttggctt
     7981 gttgttcagt tgatactctc atatataatg ctactcgttt catgttatct cctttcgaat
     8041 gtatgttctt atcgttgtaa aagaaaagcc cgaaggctaa tctttttact acacatctgt
     8101 taattctaat gatcttctgt attgttcagc ttgagctagt ttagtaaaag atacagttcc
     8161 gttataattt tctaaaacaa ctttttcaat ttcatcaaga gatactttaa agaattcttt
     8221 tcgttgatta actttattga cttcgttttt tctaaaatgc gaatgtagtt gattttctaa
     8281 tgttggtgca tcttctgaaa atatcattgc atgaacatca aattcaaaag gtacagaagc
     8341 gctactaagt tccttaattc tatccatagg ttctaaccgt ctagtcattc ctattttata
     8401 gacatcttca ccgaaagatc cgatattact gatgacataa acatagccag ctctagtatt
     8461 aagttctctt tgcaatacat cttctttgac agtttctagt tctcttaatt tctcttccag
     8521 ttctttaatt ttatcagcat agagttcttt ttcaacatca gcactagctt tttgcatgta
     8581 tgccattaac ttagttatct ctgagttaaa ctgtcgttct tctttatcaa tgcgtttctt
     8641 ttcacgttct atttcacgtc ttaccttttc ttcttcaatc atttgttctt ttattgcttt
     8701 ttgctgttct ttttcatcgt taactttaac tatgtatgaa tagttgagtt ctaattcttg
     8761 aagttttaat tcaagtaatt cttttttgaa ttctgcttca tcgtttttaa aaagcttgtt
     8821 tattttatca aaagttctta taattttttc tctagttgaa tctacgttag aaatagtaag
     8881 tttattgaga agtccagtta tttctgattc taatggtata agcattttct ttttaatatt
     8941 attagcttct ctattattca aataagaagg aatattaatt gcaccgtcta aaataaaatc
     9001 cttttcttta atctttaata aagaaagttt gttttttatt tcggatgaag aagtttcatc
     9061 tatattcgga ataattactg atttatagat agtgaccaga gatgtgtcat atttttgatt
     9121 aatttcatcg tctaaccgct ggctttcagc attaagaata tcaattttgt tttctagcgt
     9181 tgacaagttt ttattcaatt tacttatgtt tttctctttt tcagatataa acgtttccct
     9241 agcatttaat tcttgagaat attcctttac cttactctct aaggcagaag ttttatcttt
     9301 taatttttta gcttcagata atttgctact acccataatt attaaaacta ttccaactaa
     9361 aaacaatagt actcccattc aaaacgcctc gcttctttta taaaataatt gtcaaaatca
     9421 tataaaccct taccgaagtt gacggctccg gcatgggaat ttttttaaaa tacaactttt
     9481 ccgataattc taatctgttc tggcgataca attctatctt cgtataagtc gtttatagaa
     9541 cgtaaaatga ttgtttgatt ttcgtaatct ttatatatct ctttacatgt aactccgtca
     9601 ccacctaatt ccacaatagc aatttcccca tcttctacgt ccggttgttc tttaacgaat
     9661 acgaattgtc cattcctgat aacaggttcc attgaatcgc ctataatagg tactgcgaag
     9721 tcagcagcac ttggaactct ctcaaattct tcctcttcga caaccgtatc accgtaagta
     9781 agttctgttg gattggcagc cgaagccgaa ttgtgaacag ttgggatttt ttctttttta
     9841 ggaaaatcta ggacattttc ttgtttttgt tcttctaatc gattacttgc aaattcatag
     9901 gtagcaactt tattttcgtc gttaagctga ttatatatag gaaggatatt atatatctga
     9961 ttagatatag ataaatcaat attcatcaaa tcatctatat caacattgaa tatcttagct
    10021 atctcattta aggtttttaa ttttggtgta tattttcctt tttcccattc gcttattgag
    10081 gatgaacttt ttcttcctag tttttcggct agttcttgtt gatcaatatt atacttttct
    10141 ctaagaaact ttagattttc agcgaacata gtaacacctc cttgagttaa ttgtaacacc
    10201 gtttcggaaa aaatgtaaca ggtttcttag aaaatatttt agtgttttta aagaagtatt
    10261 tacgcaaaat agaaatcgat ttcaggaaaa ctgaaatatg cacttgactt cggaaaaacc
    10321 gaagtatata atttagtcag aaagttaaag agaggaggcg aataaaatat ggataccgcg
    10381 aagaaagaaa aatatacatt gagacaatta agagctttaa agggttattc aaaagaagaa
    10441 ctgtcaagga aaagcaaagt gacatcaaga acaatattta tttacgaaaa cgatgtagat
    10501 aaaatgagga acggaaagta cgccactcta gaaaaaattg ctaaggcatt gggtgttaga
    10561 gtttccgata tttttttaga ccctgattcg gaaaaaccga aatcaataac aggtgaaaca
    10621 aaacaagtca gctaggaggt gtgaatagtg gaagagaaga atataaaagt accgactctt
    10681 gaagaatata caaaaattgt tgtcgaaaca gacgagcaga acccgaaaac aatcgcagtt
    10741 attacggcga atgatattga atcatgcgaa ggttttcgag ttcgaatgac ccctagatat
    10801 gattaatgtt ctttatcacg tggaggtata ggatcgtttc caaaactatc ttttgattgt
    10861 atttgtccat tctttttttg gacaataaac tctgattgct gttttttaga aatcttttta
    10921 ccatactcaa cagcatctga tttattatca aatagttttg tatcttcgga attaccttca
    10981 ccacgtacag cccatttgcc atttctagga gatacccatt gatttttacc cataacaatt
    11041 cacctcgctt tcaagttaat tctattaaga ggtgaaaaga aaacaaactg atggaggtga
    11101 gattgtgagt aacaagccaa aaatcacttt gaatggcaca cctaatcttc aaagattagc
    11161 agatgaaatg gctagaatac gttctagaca ttggggagtt actgtggtgg caaccataat
    11221 tgaacaaaaa gaaaagccca cacagccggc aagcaagtag tgggcaccat agaacaataa
    11281 tattatatca cacactcgcc acttccgaaa agaggagttg ctgctctcct ttcaagtttg
    11341 ccaaagtcca cgtttcttca ttttggcaac tcctcttctt ggaggtggtg acggcgagag
    11401 acgaaggagg gatgacatga aggaagaatc aaaaataggg attttgaatg agaacaatta
    11461 cacaagatta attattgaaa cagaagaagg ggaaaaagta gcagaagtta cattgaccga
    11521 tgcaactcct gctgacggtt atcgtattcg attaactcct aaatatgatt aaccttttgg
    11581 tgggtgtgga tcgtttccgt gactatcacg gctttgaatt tttccatctc tgccatgaat
    11641 aaatagctca gatccttgat ttttagaaat ctctctagcg atttttactg cttctgattt
    11701 tgtattagta tgtgcagtcg ctttagaatt tccagcacct ttgacattcc aaccaccatt
    11761 ggaattaggt acaacatgtt gattttttcc catagttttc acctccattt ctatagaaat
    11821 attttatcaa atagaaatgg agaaattcaa taaacgaaag gagggatcaa atgacattac
    11881 aagagcatca acacttgatg cgaaaactaa atcaggaata ccacgaggaa atgaaaccta
    11941 gattgctagg ggaacaatgg cgtgaacgcc agaagaagtg gttagaaata aaaaagtgac
    12001 tcagccgtcc aaagcaatga gtcacaaacc aaaaattata cctaaggaga ttttatcaca
    12061 tgaataagaa aattgaaaat cttatcgaag aattgaaacg tgaatgccaa aagcaaggtg
    12121 tttctattat ttgtacagcc caaaaagaag gggaacttaa aagtctcgtt tatggtgaga
    12181 caacagaaat tttactttgt cttgctatgc aagaagaaca tttagatgaa aatctcccat
    12241 tgtcagcaca tattatgcgt agaattgctg tggatgctta cgaacaagcg aaaaatgaag
    12301 aagaaaatca accttcaaat catacttttg ttattaataa caaggaagat ttagctgatg
    12361 tgatgactcg tattctcaag ggggaatttc aatgatgaat gaaaaaacaa ttgatctact
    12421 cattatttta tttttcacgt tagtgattgt agcgaaaagt tatgtaatct ttggcttgtt
    12481 attcgcaatt accatgcttg tgctttgcgt gttagcaagc agaaaagagg ttaaacatga
    12541 acgagttagc aaatcttgac aactatttaa ctgatcctga ttatgctaaa ccaccatatg
    12601 aagcgccaat tgatgaggag gatgaagatg agtaaatcta ccttagaaat gagccatcaa
    12661 gaatggctcg aagaccgcaa aagaggtatt ggtggctcag atgttgcaac cgtacttgga
    12721 ttaaacaaat acaaatctcc ttatcaattg tggcttgaga aaacgggtca aattgaattg
    12781 aaagatttag aaagcgaacc agcttattgg ggtaatgttt tagaagaagt ggttgctaaa
    12841 gagtttcagg aacgtacagg caaaaaagta cgcagaagaa accaagtatt tgaacatcca
    12901 ttacatccat ttttaagagc aaatattgat cgggaagtag tgggagaaaa tgccattctg
    12961 gaatgtaaaa cagccaatca atttctcgga aaagagtggg aaggtgaaga ggtaccgctt
    13021 agctatctct gtcaagttca gcattacatg aacgttttaa acaaagacta ttgttatatc
    13081 gctgttttga tcggtggaca aaaattcatt tggaagcgga ttgaacgaga tcaagagctg
    13141 atcgatacaa tcactgaaca attagtagag ttttgggaaa cgaacgttct tggaggtatc
    13201 gagcctgtaa ttgacggaag tggagcgact gctgacttct taaaagaaaa atatgcagat
    13261 gtagaagaaa atcaaacagc tctaccatca cgttttgatg aacttatcga gcaaaaaaga
    13321 gaactcaagc ggacgaaaaa agaaattgaa tcatctattc gtcaagtaga caacgagatc
    13381 atcagtgagc taggtaaacg tgaagccagt atcggtatca cacaaagaaa cattatcagc
    13441 tggaaacttg ttcgtacaag acggatgaac acgaagaaac tagcagagaa atatccagat
    13501 gtcgcaaatg atgaagagat ttataacgtt actgaatcaa gaaggctaac cgaaaaggag
    13561 atcaaataat atggcaacaa atgaatcgtt aaaaaatcaa ttggcagaaa agccacagaa
    13621 acaagttgca ccaggacagt tagggcttaa agctctaatg aatacaccaa caatgagaaa
    13681 gaaatttgaa gaagtacttc atgacaatgc taatgctttt atgtcgaatg ttatgactct
    13741 tgtatctaat gacagttatc ttgcagatag tgaaccgatg tctatcatga gtggtgcgtt
    13801 aactgctgca acattaaatc ttgggctaga taagaattta ggttatgcat atttagttcc
    13861 attcaatagt aaaaacaagc aaacaggaaa atgggaaaag aaagctcaat tcatgcttgg
    13921 ctataaagga tatatccaat tagcccaacg atcaggtaaa tacaaagcat taaatgtgat
    13981 cgaagtttac gaaggagaac taaaaagctg gaaccgactg acagaagagt ttgagtttga
    14041 tccaaatggt agaacgtctg atgaagtcat tggatatgtt ggctatttcg agttactgaa
    14101 tggattcaaa aaaactgtct attggaccaa acaagaaatc gaagctcatc ggattgctaa
    14161 caataaagat cgcgataaga caaagttaag tggtgtgtgg gcatctgatt acaatgcaat
    14221 ggcacgaaaa actgttttga gaaatcttct ttctaaatgg ggaatcttgt ccatcgaaat
    14281 gcaagaagcc accacatcgg atgagagagt ccaaagggtt caagaagacg gcagcattat
    14341 tgctgaaaca gaagttgaag aagatattcc tgaaagaaaa gaagcagagg ttatatctga
    14401 agaaaatgaa gatgtacaaa ctggattatt tgatgcatct aatccgccgt taaacaaata
    14461 atgagggagc tttctccctt gcttttctag aggagaaata cgaatgaaca caggatatgt
    14521 caaattacat cgaaaagtga tgaattcatt cgtttggacc aatccttata tgtataaatt
    14581 atggagcttg tgtttgatga aagcgagtca tgagaatcgc aagatacttt ttaatggaaa
    14641 agagatacaa gtgaacagcg gagaattcgt cacagggcgc gatgctatca catctgagat
    14701 gaacgaaggc gtgaaacgtg aacatcaagt gaacagcggt tctgtatgga gatggctaaa
    14761 acaatttgaa aaaaacggaa tgttgaacat caaatcaact acgaaataca gcgttatatc
    14821 aataaaaaac tggtctttgt accaaagcag tgaacaacaa atgaacatca aacgcacaac
    14881 aagtgaacaa caagtgcaca caaacaagaa tgaaaagaat tataagaatg aaaagaataa
    14941 taaaacatca tcgtcgcaac cacgcaaaaa gcgtgtttac gacaccgact cagtttacta
    15001 cattctcgcg gaggagttat tcaaacagat ttgtcagaat caggaaatca aaaagccgaa
    15061 tctgcaaagt tgggcagata acattcggaa aatgatcgag atcgacaagc ggaccgaaaa
    15121 ccaggtacgg ggaatgattg aatggagtca gcacaatgtg ttttgggcat cgaatatttt
    15181 atctgctaaa aaattgcgag aaaaatacga cacaatggca gcgcaagcaa atcgtgatta
    15241 taaaacaaaa caaactaaaa cgcttgaata cgagaaattt agcacagatg agttgcctat
    15301 ttgagaggag gcgtaggcat ggaaactgtt ggggaaatca tggaaaagct gatacagaaa
    15361 gtgcttgtcc aacgtggcga atgtcctgaa tgcggacagc ctttgtatgg atggcgtacg
    15421 aagaaccctg atggctcaga acgttgtaag ccaacctgta tgcaatgtgg gtataaagca
    15481 ttgcgagttc aggaagactt acaaacagaa cgaatttaca acgagagcct gaaagcaaga
    15541 gcaatcaatt ttttcaaagg tggttctgtt gtgcctaatc aagcgttgtt tgattgcaca
    15601 ttgcagaatt atcaaattgt cgatcaagaa acaagacaag cggttgaagt aaccaaacgc
    15661 tttgttaatt cagtcttgtt aggaaatcca agtcaccttg ttttaactgg aaaacaagga
    15721 acaggtaaaa gtcacctagc gatggcagcg gcttgggaag tcttgaagcg atcaaactac
    15781 gataaaaaaa tcttatttat cgggttacag gaaatgctgg atcaaatcaa attttcgtac
    15841 aacaatcctg aactcagaaa aacgattgag ggatcgttga ttgcagatat caaaacagcg
    15901 gatttagtca tcattgatga tatcggttca gaactaggaa aagatgcatc agatagtcga
    15961 gcgtttggca taaacacgct aaattcgttc ttggatgcac gacagaacct agcaacgatt
    16021 atcacaacga acttgcttgg tgaagaactg aaaaaagctt acggtacgag aacaatatca
    16081 agaatgtttg tcaactctga tggatttacg atggtatttt ctcaaacagc agacaagcgc
    16141 ataaaaccag tcaaaggagc gatcgcatga atttgactaa acaagaaaaa agaatctgtc
    16201 gtcagataat gttgcaaatt ataggtgatc gtgacatgga cgaattatct caacaagaga
    16261 gtcgaatggt tcggaagata agtaagctaa tcggtgcaga tacgatggat cgatccaagc
    16321 cattacccaa actgaatttt gaatcattta cgtttgagaa atatagccat ctgagagagg
    16381 taggctatag tgtaaatgct attcgaaaag cacttggggt aggaagtagt gtatgggcaa
    16441 aatggcggaa agaaaatgta aatgaggtaa atcaaaatga ttaagctaat aggcaaagag
    16501 acgctcaagg tttactatgt agcggaaaca aaggcagaag tcagccgttg gctattgaaa
    16561 atgtttaata agactagagc caatgcttca gtaagtcaga aagcaagtgt atcagaaaca
    16621 ggcatgcctg aagcaatgtg gatttcaagg aggaactgag atggcaatga taccgaagtt
    16681 tagagcgtgg gatagagaaa aagacagaat gatttatcct agcacgacag gagtttgttt
    16741 tgaaatgaat gatgatggta taagtgtgct tgatattgat gtagattatc ccaatgacta
    16801 tggttttccc aaaatagctt caatcctcat gcaatctacc actctgaagg ataagaatgg
    16861 tgtggagata tttgaggggg atatagcgct tatcactcat aaatcaacga actacgccga
    16921 tacttattgg cacagttacg tagaagtttt tagagcaaaa aacggagcct acagaattcg
    16981 cggtgaacac atctacgaaa cagaattata tagtaataga aaacaattga cggtagcggg
    17041 gaatgtttat aattcaccag aacttttgaa gagagatcga tttatgacta ctaccgaaaa
    17101 tccagagtta ttgaaggagg aacagcgatg aataaacagg aattgattga taaatatact
    17161 gcagaaatat ctagattaag accttattgc cctaatagac atttaattag cgatcaactt
    17221 aaatatggtc tttataaaga aatcctagaa gatttaaaac aactagacga accgcagaaa
    17281 ccagcggtgc ctaagtttgt ggcggaatgg atagaatacg ctaaaaagaa aggcgatagt
    17341 ctagctattt cattcaagcc gtggaacctc tacggtgttg agtatagcaa gactgataga
    17401 tggattgaag ataatcaagg aacgtttgct cgtgcttggc taattggcta cgaggtcgag
    17461 aaagagccgt tgtattatgt gaagttgcca gttgtgtatt ttaatcattg ggatttagag
    17521 acgtatctaa tgaaagatga tagaggaaat ataacaattg cagacaacaa cgattttgat
    17581 gatatgaaat ttacggaatc agaaataaaa gcaattgatg aaagatactg gccatttgct
    17641 gtgccagtgg aagaggtggc ggaaggatga gcaatcttaa agttggagat attgtttttt
    17701 acaaaggtga cgaaggcatc atccgagaag ttatgacgga tggaaaagaa aatttttata
    17761 gggtagatgt cagcagaaaa tctatactct acctgtatga agacgaatta gatttaggta
    17821 ctcaaaaact caacgacaac cagaaaatgg tgtttgatta tcttaaatca aacgttgaac
    17881 aaggtggcaa atcagttatg tattcgatat tccttctagg tgattgggac tctcgaattg
    17941 gagcagcaag aaacgttgat atagcttact gtggattaaa tggtaagcag gaagcagaag
    18001 tcctatcagc attcgctcaa tggggattag aacaggagga agcggaatga aatacgaaat
    18061 accactaagt gaagcagacg tccagtcaat tattaacggt cgggaggtta ataaaaaact
    18121 tcctgatggt actgaattag tcatcagaca aagttatttg aaagatatgg cagctccagt
    18181 attaattgat cgttttaacg tgactgattc tgtggtagag aaccatttaa aagaatttcg
    18241 atcaagtata gacgacactt tcagattagg gagttgattg acaatgaaca acagacatcg
    18301 cagaataaca aaactaagaa aacaggaact gaatgtacta aagacaaagt ttgaaaaaga
    18361 atatggaatt tcagcagaag aaacatataa agtggcaagt cagtgtgttg ctgatgcgag
    18421 taatgctatt cgtaagtttg ggatttcgat attaaatgat gatcgtaaat gggaggaaat
    18481 gagatgaaac taaaagacgg attttacgct agtagtcacg gcatcggcgg tttaatgcta
    18541 gatatgccga caaagaatcc taaaacacgt aagaaaccaa aagtcaaagt cggtgacatg
    18601 gttcgctgcg aagcagaagg gttcatctat ccatttcgtg gatatgtaga acacgtctat
    18661 aatcactcag caatcattcg tattgaaaac acgatggaat gtgataaatg gacagctaaa
    18721 agcaaagaga atttagcagt ggtgagattg gtggatattg aactaatcaa tgacaaataa
    18781 aaaagccgga tcgctccgac tgattcaata aattccacaa gtttattata tcacataaaa
    18841 ggggcggttt gacttgatgc aattgttacg agaggtagct ttcaaacaga caagatgtaa
    18901 tgcgagagat gtgctgaaga actttcggcg tttggagcgg atggcaggtc gctctttgat
    18961 agatattaag tcgccgatta ttacggacat gccgaaggca ccgaagcacg gcaataaggc
    19021 agaagacgcg atcattcaga tgatggatat agaagcagag agagacgcga ttttagcggc
    19081 tttgatggcg cttagtctga ttagtcgtca gatactctac tacagctttt gtgtgccaga
    19141 tagcttctca aactacagga ttagccgtga agtgggttat tcagaaagaa gcatacaacg
    19201 gatgaagtcg gaagctctga tagagtttgc agaagcatat aaacatggga aaataattgc
    19261 ttataaatag gaggactata taatgtggaa agattatgta tcattgaaag agttgaaaaa
    19321 agatcttatt ttcaaaaaaa tcgtagaatg gtcagagagt gaattaattt tagaagatgg
    19381 aaccaaaatg gaagttgtat gtagcgaatc agattgctgc gcatgggccg aaggtgaatt
    19441 caaaaatgta aagcttgatg cagtgattac agatataaag atttttgata aaggtaaccg
    19501 tctctataat ggcgatggac atagttctta tgccgaagtc gttgtttatc ataatcgaaa
    19561 tgagattgct aaagcggaat gtacagcaaa cgatgggaat ggaggctact attacagtgt
    19621 ctgtgcttta aaagtcaaag aaaaactttg tgtagtgact gatgcataaa aaatggcggt
    19681 tttttggcgg aatgatggcg gtttttagct atttaccagt gatattatgg tagtgtcgaa
    19741 agattagtga taggtctgag acaaaataaa atgtaaggga ggaaatctcc ctcatcgttt
    19801 aattaagctt cgatagacag cagcggaaat attaagaata aggatgtgaa ttttaactcc
    19861 ttctaaattg ttcttattat ctatcatccg ttgctgtcta ttgttattat gtcactgtgg
    19921 cggaaagggt agacgcttaa aaataaggtc aatacgtcga gggatagcct taacgtttta
    19981 tgatttgacc atgcaaggtt cgattccttg ccagcgactt agacgaaagg tagcttaaat
    20041 attaaccaca tcaacatcgt cagcgagttg ataaaggtaa ctagcagact ctaggattta
    20101 agccagtctt accgattagt ctgttatagt agcaagcttg ctagaggtag ctcctctggt
    20161 tgacgtgcag ctcagttggt agagcgcctg acttttaatc aggaggtcgc cggttcgaat
    20221 ccggtcacgt taataaagaa cctacggaaa caatcaatct tatcggatgc cgattggttg
    20281 gctgaattaa gcagtgtgtc tgtgaatcag ttaataggca taaaatacta gcacagacgt
    20341 gtgccactct caggtgtagg ttaggagaga aacattagca accgagggtt ggaaatgggc
    20401 gctcaaagta cacgagcaag gcgaggtcga tagtaatcga tggaatcggt gtaggttgct
    20461 attacataat tgattgggtt agattgagtt ttgggattcg gtacaaatga atcgtcaaat
    20521 gactcaagca caggatcgga aacgtccctg cctgtgcatt acatattaga tcactcgttg
    20581 agtggtcttt ttattttaga aaagagagga ttttgaaaat gaatcatgag aagtttatcg
    20641 aaaaatgcaa ggctattgta cgtgaaagaa ttgagaatga gattgctgac ctaagtggag
    20701 cagtacctga gtttagtgtt ttcatagtct ggtcatgcaa aacactacag aatagcaaag
    20761 cattagtcag cgctagctta aaaggagcac cgtattttga aattacgttg aacggggaca
    20821 aaggtgaaat ctatgtagat acttatctca aaaaatcaaa tgaatgtatc aaagtctagc
    20881 aggtgctagg ctttttcttt acataaagga ggaatgatct catgaaacaa tataccgcta
    20941 aagatttcga ggaaatgaag cgattaaaga aggactatga agaagttgat atggagctaa
    21001 ctgttggagt cattcaacga agactgcggg tcggattaga gacagcaaag gctatttaca
    21061 atgatctaaa tgctattgaa gagaagaatg gctaatgagg aactactggt atatatcgct
    21121 aactaatgaa tatcctcgaa ccattgatga ttgttcagtg cgtgttgtgc gttctgtaca
    21181 aatcaaaggg aagtactcta ttgtcgaaat ggggagagaa gcagaaccat gtgagattga
    21241 tgcgtgcaag ctcgtttatt gcggtcatgg tttctatgat gaaccaaaca ttcaaaataa
    21301 tattaacaag aatttgaggg attagaatgc aagaaatggc ttaaaaatct ccaatcagga
    21361 caaatcaagt tagacaaagt ttccgattta aagatattaa ttgaagcaga tctaatgttg
    21421 aaagatattg aaaattagaa aacaaaactc aacctaagaa gattgcgagg tggtgtgtat
    21481 tgaatggcaa gacaacgtga tccaagacgt gatgaagcca aaagaatttg gttagaatcc
    21541 aacggagaaa agcagttaaa ggaaattgca tctgaattaa atgtttcaga ttctcaggtt
    21601 agaaaatgga aatcgcaaga caaatggagc gctgaattga aaagtaacgt taccaatggc
    21661 aaaagtaacg ttactaatca aggtggcgct cctattggta atcaaaatgc taaaggtaac
    21721 aaaggaaata gccgagcttc tccgccagtg ggtaataaaa acgctttgaa aacaggcgag
    21781 tatgaaacca tattttttga gacactaagt gatgaagaga aggacatcta ttctagtctg
    21841 aatgataatc cttcttttgt tttgtctgaa gaaatacgtc tacttaagat aagacaattt
    21901 cgtatgatga agagaatcca acaagctgaa gctggactaa atgatgaaga agtcgaacga
    21961 ttgcagcagc taagaaagat taaaaatccg attgaaaaaa atggtaaaaa gctagaaatc
    22021 aagcgtgagg ttatgcaaga tgtgcagatt agcagaaaaa aacatcgcaa aattgatgat
    22081 attctttcaa ttgaagattc attgactcgg attagcaacc agttagctaa agccatcaag
    22141 caaatgaatg aactttatat gaatgaatac agaactgatt taattaaagc tcagactgat
    22201 aagatccaag ctgagacaaa tgaaattggc ggaaataatt caggtgaaga aatagaagaa
    22261 tggaaacagg cagttttaaa tgccgcaaac aaacgggcgg tgaaagaaaa tgaataatga
    22321 atttattcct tttgctgata ttggttctgc cattgattat tactatgata aaccagtagc
    22381 tttttgccaa gatattttgc atttgaatcc tgatgaatgg caagaaaatg ttttaaatga
    22441 tttagctgaa ttttcaaagg tttctgttcg ttctggtcaa ggagttggaa aaacagcatt
    22501 agaagcagga gcaatacttt ggttcttaac gtgtcgaccc tacgctaaag taatagcaac
    22561 agctccgaca atgaagcaac tttacgatgt actttgggca gaggtagcta aatggttaaa
    22621 tgatagcttg atcaaaaact tactgaagtg gacaaagacc aaaatttata tggttggtga
    22681 ttcagagcgt tggtttgcta cggctagaac agcgactaaa ccagaaaata tgcaaggttt
    22741 tcacgaggac catatgttga ttgtggtaga tgaagcttct ggtgtgtctg atccaattat
    22801 ggaagctatt cttggtacgc tatcaggttt tgataataag ctgttgatgt gtggaaaccc
    22861 caataatatt gaaggtgttt tttacgattc ccacaattca gaccgtgata aatacagagt
    22921 tcataaagta tcaagctatg atagtaaacg tacaaacaaa gacaatatag aaatgattct
    22981 taaaaaatat ggaaaagaaa gtgatgttgc tcgtgtccgt atttttggag aatttcccaa
    23041 aggtgcgttg gattcattta tcagtcttga aacggttgaa ttggctacag aaaaacaaat
    23101 tagtgattct ttagtcaata aaacaacggt tgctcatatt ggtgttgacg tagctcgata
    23161 tggtgatgat tctacgattc tctttcctag aattgctacc agggcattgg agtatgagaa
    23221 gtattcaaaa cgtagcacca tggaaacaac aggatatgtc atcaacatgg ccaagaatct
    23281 aatgagtcaa tatccgagta ttgataaagt gatgattaaa gtcgatgaca ctggtgtcgg
    23341 aggtggtgta accgaccgcc tagaagaact tatagaagac aaacattatc cttttgaggt
    23401 gtttggagtg aataacggtt caacatcaga agacgatttt tacgataatt taggtactca
    23461 actatgggga aacatcaagg aaatgttaga agaaaatatg acagcaaatc ttaacggaga
    23521 acagcctgtt attgaattgc cttctgatag ttcgttaatc aaagaattaa gtactcgcaa
    23581 attcaaaatg acaagtagaa gtcgtatacg tttagaaagt aaagatgata tgaaaaagcg
    23641 aaatattggt agtcccgata ttgctgacgc actggcttta gcgttttatg agccaccaag
    23701 tcactatcaa tttattcaat tttaggaggt gagcttttga ttagtacagt attatcttta
    23761 gagagatata agaagctacg tgttaaatat gcaacacaaa ttgaagatgg aatgtttgat
    23821 ccgaatggtt ttatagaaga tatgaaacca ttttttgctg atcgcgaaag aaaatatcta
    23881 gcttatacta gcgaaaagaa tgaaatagat aatagaccaa agcctaacac tgatattgta
    23941 aaagttaata ataaacttca tgctggtatg tattcaattg tcgtagacca agcagtcaat
    24001 cattttactg gtatacctat caaatgggat tacgatgtat cggaacaaaa aagaacactc
    24061 attcaaagat taaaagataa attcttaaaa aatgatatag aacttccgac agttccagaa
    24121 gcttttaaca aattaacaag caatcttgat tccatgagat tcgcaatgct tgattctgaa
    24181 actgcaactt ttcaaggagc ttgtggagta gctttccgat tgttagagcc tgtggaagaa
    24241 gatgatggtt ggaagttaag agcaagtaat attgaacctt ggagagcaga aagatatgga
    24301 aatgccggaa tctatatcaa agaaaagtat gactcttacc agaaaaaatt ttttcaagaa
    24361 atgaaagtta tgacgagaaa taaaattctg acatatgctt gctatggtga tttgaatttt
    24421 gttacaagcg gaacgttcaa aaaaattgat gagaccgata accctttggg gacgattacc
    24481 ttggcagaat ttaaaaataa tacgaatcgt tattgtgatt ttgaagtagc tgaggaaatt
    24541 ggagatgcaa ttgatcgtgc tttgtctgat caacaaaacg aaatcgaaca atttaagctt
    24601 gcgtacatgc ttgttactgg cacaacaatg agcaaaggca ctgctaaaga aatgatgaat
    24661 cagctaggga ttatcaactt aaaagaccct actgcaaaag ctgaatatgt cacaaaaaat
    24721 ttagcaaaag atttcaatga atatcatatg gatctattga aaaaacagtt ttacaccatt
    24781 tgtaaagcaa tcgattttaa cgatgaggtg tttaaatcaa acagttctgg agaagctcgc
    24841 aagtggcaaa tcattagcct agaagcgaaa acaaatacta aagagcaata ttttagagaa
    24901 ggattgaaag aatgcgcaga aacaattgct gcttttctta agtttcatga caaagtagaa
    24961 attgagcctg aaaaaattat ttttactttc tctagatctt tgccaactga tttaagttat
    25021 ttagctgaag ctttacctaa acttgcacca tacgtatcca aacgaactat tcagagccaa
    25081 attccatttg taacagatgt tgattatgaa aatgaaatga tggaattgga aagtggaagt
    25141 gcttatccag atagtgaata caattttggc ggaggtggca atagtgacga tagaaacgaa
    25201 gtattggacc aaacgtcgag aactggagga tcaagcaagg ctcaaacaag aaaatcagac
    25261 acttaaaaaa ttaactagtg tatttcctga agcactgaaa gagatacaag caaaactatt
    25321 atcacaagct gacttacaca atatcactta tccagaaatg atggagtttt atagtacaag
    25381 taatcagaaa aaatatcgtg aatatgtgga aaaaaattat aagtcattaa aaatgtatga
    25441 tgcaaaatac aaagagttta tcgatgaatt ttttccacca tttgactatg caaaagtcaa
    25501 tcgcttatta caaatacgat cagatgtatt taaaattctt gcagaatatg cgatggatgc
    25561 agatgtgaat caatattttt ctgatcgctt agaggaaatt cttcaaagaa catattcttc
    25621 taatgctaat gtttttgttc aacttttaaa cgttgatata ccaaattatt taccagaaaa
    25681 tgaaattaaa cattatttga actatccatg gtcaggaaag acattttcaa gaagactttg
    25741 gggaaatatt tcatcgcttg aacagaaact ctctaatgct attgtaaaaa gtgttgctag
    25801 tggagaaggc gttatacacg cattaaacac catgagatta gactcagaaa tttgtgacat
    25861 gtttaagtta gaagaatcaa agtataacaa agcgatagaa aatctcgttc gaacggagta
    25921 tgcaaaattt gcacaagatg gtattgaaaa atcatattta gaaacaggta ttgaggaata
    25981 caacgtattg actgcaaaag atgagagagt ttgccggatt tgtggaggaa aggcaagtaa
    26041 gaatccctat aaactgaaag atgctgtcat aggtgaaaat cgagcacctt tccatagtcg
    26101 ttgtagatgt acggatgttc ctaatttacc aaaattagga aaggatattg atgaagaata
    26161 tgaccgttta tttggcgatt tattagatga gtttgcacat gattcttttg gaattaattt
    26221 gaaacggagg aagtagaatg aaagattttt ttgaagcagt actaacaatt aatgtaaatg
    26281 ctgatattgc agaagcctac aaaacagcta ttgagtctga gaaccatcct aatggcttga
    26341 gagaccattg gaatggcaat tatgcctacg tggttattgg cgatcaaact gttaattatc
    26401 aagataatac tccagttgat aagaataccg ttaatttaac gattcaatta ttatctcatt
    26461 cattaccaaa tttaaaagaa acagttgatt ggtatgaaaa gatgggatgt attgttgtta
    26521 gaactgacta caaagaagga aagtctagta attaggcttt tttattttgt ccgaaatgac
    26581 gttaaactag cgcaatactg ggcttggttg aatggtgggg cgcaactatt aaaactcaaa
    26641 gcaatgcggg gcgtgaaaac gaatcgtggg gcgaaaggag aatgagcatg aaacacaaat
    26701 cattaatgcc aatgaatttg caatactttg ccgaaggtga cgatcaaaag ttttcttttg
    26761 atgacttcaa atcttttgtg gaatccaatg aagaagctca aaaatttatt cagtcacaat
    26821 ctcaaagtgt tgccgacaaa caattggaag cttggaaaca aaataactta gataaaatta
    26881 aacaggatac catcaaggaa tatgaggaat ctaagaaaaa caagtcacct gagcaaattc
    26941 aactggaaaa actacaagct gaatttgaag cagaaaaagc gttgcgtgtg acaagtgata
    27001 ataaagcatt tgttgcagaa caaattgctg gattagaact agatggagag ttaaaagagt
    27061 ctatttctca atttatgcta aataatcttg ttagttcgga tacagatttc actaagaatg
    27121 ctgttgaagg ttttacaagt gtcttgaatg caattaatga gaaacatgca gatgcattaa
    27181 aagaactaca aatgaagtct gcatttggtg gaactcaaca atcgaataac caggttcagc
    27241 agaacaatga aacattaaca aatccagaag aacaattagg acaaattctt caacaattta
    27301 actaggagag tgaaaaatta tgaaaaaaac atctttaaat aatttagagt atttggatat
    27361 ttcaccagcg attaatgcta tgcaagtacc aaatacacct tttttaagct atttatttgg
    27421 tgctggaaaa acagagccag caaactcgac agaaattaaa tggcgtgaat atgatatcaa
    27481 caacgatgat tcttctgaaa aacttgaggg cggagaatat ccagatgctg aatcaggtcg
    27541 aacttggttt aacaactata ctgaaatttt tagaaaatca acctctgtat ctggtacatt
    27601 agatgctatt aatgtgaatg gagtcggaaa tgaattaact aatcaagtag cactacgtgg
    27661 tatggaaatg aaaattgatt tgaaccgaaa attgattact ggtgtaaaag ctgatgaaaa
    27721 tggttctaaa ggtcgtcgaa tgaatgggat tttgaacttg atcaattcag caaataaggc
    27781 agaaacagcc actgcgggtg cagtaacaag aaaagatatc gatactttat ttaaattgat
    27841 gtatgaaaaa ggttatatgg gagaaaaact atgcttgatt tctccagata tgcaggagtt
    27901 aatgactgat gagttagatg gaaaatcaac aaaaattgtt cagttcgggg aaagagtaac
    27961 ttttggattg caaattggaa atatcgtgtc taattacggt acaggtattg ctctactaga
    28021 accatcattg ccaaaaggaa caattgccgc aatcgatact aattatgtga aactacgtcc
    28081 attacgtgaa tggagagcag aagaacttgc aaaaacaacg gattctagac gtatcggtct
    28141 tgtaggtgaa tactctcttg aatacaatgc ttcaaactct ggggcaattt taaatttaaa
    28201 gtctgaataa aagggagtta gtactccctt ttttgatagg aggaattaca gtggtaaaaa
    28261 aagatgaaac taaaaaagat gaagtcgtga aatatagagt aggtaaaact aaaaattttg
    28321 ttgggtttgt tcatcctaaa actcgtagat ttatcacagc agattcaaat aacgaattta
    28381 tcatttctat agatgataaa gaagcaattg caattttgga agatgcaatt gatgttaatg
    28441 aaatttagga agtgatctga tggatgaatc gctaaaaacg gaaatcattg agtctacaaa
    28501 agaagatttt ccagatttga gtgaagaacg cataactaat ttattagaaa taattttgct
    28561 agaaattgaa tcatacaaca cttgtaaaaa tgatatttca tgggaaaagt taaaaagcgt
    28621 gattagtgaa gtgttgtata aaataataaa aaatgaatca gaaaaaacag tatcttcagt
    28681 tagacgtggc gatacaacga ttagttatgc ttcaacaaca aatgatgtta gtgagttact
    28741 tctaggctat ggtgatttga tacgaagagt tattggttgt ggaggattgg agttttttta
    28801 atgaatgaag cagatatttt agaaactact tatgaagata gttgtattat tgaaagactg
    28861 acggacattg aagattctaa tacaaatatt actattcaag attacaaaaa agtatatgat
    28921 aatcctattt cttgcgctct ttcacaaggt caaatcgatg gactagcagt catagaagat
    28981 ggagagatgg taaatgtttc aactgacaca tataaattat ttgttcatcc taagattaaa
    29041 ctaaaaaaag gagatcgaat aacaataact caaaaggcca gtggcttaat tttttctcta
    29101 tttgctacta agccttttta ctatcctagt cattgtgaag tgaatttgat aggaagtgag
    29161 aaaaatgggt aatctcaaat atgaatcaaa tgcagaaaag atcattgaga attttaaaaa
    29221 tatgactgta attgctcaaa aagaaggaat atccttcgtt aacgattcaa tgaataaagt
    29281 cgttagttta gctaaaccat taactcctgt aaaatcaggt aatttaaggc gaggatatcg
    29341 tgtagtaaaa gctagaaaac tatcaagcgg tcgaattgtt ggagcagtta taaacaatga
    29401 acattatttt aaatatgtag aagaaggtca taggactaaa aatggtggct ttgtcaaagg
    29461 gaaattcatg ttaactcgtg caacgaatct tgcaaatatg tcttatattc ctcgaagatt
    29521 taaacaaatg gcaataaaaa tcgttaagaa aggaaagtaa catgaaagat gaaatcattg
    29581 ctgcaatcag tagcaaatta aaagaaatct atcctgatgg gacaatatat cttgattcag
    29641 ttatgcagtc aactaaagat ttttatttcg ttttatcagt aatggaatct ggaactgaaa
    29701 atgtaggaat tgatattcaa aatgtttctt tcttaattga tattgcattg attgataata
    29761 aacctaatag aaatttaatt aatgaattag tctcacgttg tgggactttt tttaatacga
    29821 ttacaatcga tgagcaaata ttatttccaa aagcctattt acctgatgaa gcagatggtg
    29881 ttcaacatat tcgttttaca ttagaatttc cacaatatat tgaatggagt gaaagataaa
    29941 tgggagaaaa aagaagtaaa accggaatta tttctgtcga aaaacctact tggtttcctt
    30001 tggaagatga gacagggaat tttccggtat atggtactgc gttgccaatg ggaacagcag
    30061 taagtatcaa accaacagct aattatgaaa caacacaaga ttatggggat tcagtggttc
    30121 aagatcaatt tacagctttt ggtggtgcag aagttgaact ggaagcaaat ggatatactc
    30181 ataaagtttt atctacaatt actggaggga aaattgttaa aggtggtgcc ttgcgttctg
    30241 gagaagatat tgcgcaagat ggtgcgtttg catatagacg taaaaaatcg aatgggaaat
    30301 accgttatac ggtcttttat aaaggacagt ttgctctaga ttcagatgaa acatcaacga
    30361 ttgaaggaag taaagtaagt tttactcatc caacttggaa gggttcattt gtagatgtac
    30421 caggacttgg atatatgtat tcagttgatg aagatgatga aggcgtggat aaagcaatga
    30481 tcggaaattg gtttacaaaa gtagcaattc caattgaaga agcagaactt tcaggaggta
    30541 cagaataatg tcaaaatatc aaacaacgat taaattaacg aaaaaagatg aagaaggtaa
    30601 atatgagcaa gtacaattta aatctgctga atttttacca ggaacagttg tagaagatgc
    30661 tgcaggagtt atggaagaaa tgcaaacagc gactgataaa caatcagtta aaaaagcttt
    30721 aagtcgtgct tattcattta ttgcagatac tttatttgag ggacaattta ctggtgaaga
    30781 ttattgcaaa ggaattgatg ctcgtgagat tgcctcattg acaggaaaat tattgaagtc
    30841 tgttactgca ggttttgatg aaacttatac agagacgaaa aaaaagtaag tgaggcgctc
    30901 aagtcacctt catttaagta ttcgattact taccgagaat tagatataaa gacgcaatta
    30961 ctcgaagcag gttggacgtt accagaaatt gaacgtactg acttggatga gttaatgcgt
    31021 ctttttgctt ttagagatgc ggtaaaagag catgaagatg ttgagtacta tgataatttc
    31081 actcaatttt aggaggtgat actttgaata acgatgacct aattctgaag atgatactag
    31141 atgaatctgg ttttactgcc ggtatgaata atgctgtcaa aaagttgaac tcttttgatg
    31201 aaacaatcga aagaaaaagt agaaatagtg gcagttcatt aggtagtatt tggaaaatat
    31261 ttgctggaag ttttctagct agcggagtaa ctagaatcgt aggagctggc tttgatctaa
    31321 tcaagggttc cataagtgga gcggttgatc gagtagatac gatgaataac gctctacgaa
    31381 atttccaaaa tatgggattt agtaattcag aaattatgaa gaatatcgga aagaatgggc
    31441 ttttatctca aggtattcaa ggacttccta ctgctttgaa tgatgcgata agtcatgttc
    31501 aacttcttgc ttcttctaca ggtgatttaa ctcgttcaac tcaaatattt aaagctttaa
    31561 atgatggaat tcttggtttc ggtggttcaa ctgatcaagt taatgaagct gttattcaat
    31621 tatctcaaag tttctcaaac ggaaaagtag acgcacaaac ttggaactca atgatcaacg
    31681 ctcaacttgg acctactctt tctgctattg ctaaaaagat gggaattaca atgggagatc
    31741 tgaaagaagg tttgtctcaa ggtaagattt ctgttgaaga attccaaaat caattaatag
    31801 aaatggatac caaaggtggc ggaggactta aatcattaag tcaaatcgct aaagattcta
    31861 ctaagggaat taaaacctct atacaaaatg caaaaacagc tgttacacgt ggtgttggtg
    31921 aggtaataga aggattaaat aaagcattag tagattcaga tttaggtgga tttaagggaa
    31981 ttattgataa agtgggcagt tcaatggaat cgttcttaaa agtaattgct gcaaatattc
    32041 ctacagcagt atcattttta agtaatttat ttaacgaaat tcaaaaattt ggttctgctt
    32101 tgaaattcat gatgcccttt cttgttcctg cagctaccgc ttttggagcg ttcatatttc
    32161 aacttaaagg tataccagca ataataaaaa gcttcaataa ttttaagaat gccataattg
    32221 gcgttggaaa ttcattaaag attatggggg caatagctgc tgctaattca tttgttttaa
    32281 ttattggtgc agtcgttggt gcaatagctg ttttcggtta ctttatggca accaatgaag
    32341 agtttagaaa caaggttata tctatttgga atgatgtgaa agattccgta attggtgtat
    32401 taaaaaatat aaaggactgg ggaattgata cttggaattc tgctaaagaa atggcatcaa
    32461 atgcagttga gggtgtcaaa gatgcttggt caggaacaaa ggaatggttt tctaacacct
    32521 ggaaaggtat taaggatggg gccacaggtt tatttgataa aacagtagag acttcaagaa
    32581 atgcagtcga tagtgtaaaa aatgcatggt ctaatatgaa gaaatggttt tctgatactt
    32641 ggcaaagtat aaaagattca gccagagaaa aatggaatga aattaaaggt tctattatgt
    32701 cggttgctgg tccattgatt acaggtataa agaatgcatt tttacatgtt actttttatc
    32761 tagagacctt atggaataat ttagttgaga ttggtaaaaa cgtctttgag attttaaaaa
    32821 atgttatact tgcgccggtt ttgtttatta cctccatgat ttctggtgga tgggaagaaa
    32881 caaaaaataa tatgattgct gtttggaata atattaaaga aagtgccata aatatttggg
    32941 aatctataaa gaatatattt gtaagttatt ttacaaatat ttattttgct gcactcaata
    33001 tttggacagg gttcaagctt acattgataa atatttggaa tgaagtggta aatcaagcta
    33061 aatcgatttg gttaaacata aagtattttt ttattaatct ttggattgat ataaaatatt
    33121 ttgcaattca gaaatggatt gaattgaagt tttctattat tcaaacttgg atcgatttaa
    33181 aatataatgc cattactact tggaataata ttaaacagtt tttcaaagat acttggaaaa
    33241 atattaaaga tacggcatac aatacgtgga tttctataaa aaataccatg attaatacat
    33301 ggaataacat taaggactct ttctggaata ttgttactgg aattgttaat tctgctgaaa
    33361 atgcatggac aaatctaaaa aatggtgttt caaatgctat taatcgggta aaagaaatct
    33421 ttaattcgtt aagggaagtc aatttatttg aaattggcaa gaacatcatt gatggactta
    33481 taaatggtgt aaaagaaaag tggaatgctt tgaaagatac tatcaaaggt attgcaggta
    33541 atatcaaaaa ttggattaaa ggagctttag atattcattc tccatcaaga tggatgcgtg
    33601 atatggttgg taaaaatatt gttcaaggta ttatcgttgg tattgataaa gagcaaagta
    33661 agttagatca aaccatgaca aatttagtaa aaacaccgtc tgttcaacct gtagtaaatg
    33721 gaataagtca cctaccaatt acacaaacaa agcaaaatat atctgctagt gaaacaaaag
    33781 aaattcattt acatttaaat gtttatggag atttacctga ttcaatggtt cggaagatta
    33841 ccacaaaaat taaaacagaa ttgacaagac aaatgaaacg agatgctggt gcagtaggag
    33901 gaacattata tgcaacttaa gagaggacaa ttttttatta atcaacatta ttcttctgaa
    33961 tttaatgtgt atattcaaaa caggcctgct tctgtttcag ctagtcgagt aattgaattg
    34021 agagagcgag aaggaaatga ttctatcatt attgataaag gctattacag aaatgtgact
    34081 agaaagattg agtgttacta taaagcacct tcaattgatg tggtgcagga gtgggaagat
    34141 cgaatcactg aatggttaga tatgtcgtct tatagtgatt ttattcttta ctatgatgaa
    34201 caatatattt atcaagctgt agtaatagaa ggacctgaat ttaaaggaac aagaaaaaca
    34261 ggaaatattg tcccttttga atttacagtg agcattcgac catttaagga aaattacaat
    34321 ggtagattta ctattcgaca aacagaaact tttgaaattt ataacccaga gaagtattct
    34381 tcaaaaccgc ttattaaatt gagtggttct ggagatgctt ctttttatat taataaggac
    34441 aaatatgatt tgaaatcgtt ggacagagaa ttatttatag attctaaatt agaagaggct
    34501 taccgaaaat tagatggtaa tttagaacat caggatcaag tcactttatt tttagatttt
    34561 ccatttttat atcctggaaa aaatgaaatt aaatggacga aaaatattca ttcatttgaa
    34621 ataatgccta ggtggtggag aaaagtatga aaccaagaat atatagtcct actgaaacag
    34681 atttctcaac gaatggctta ggaattttaa aggattgtac aagatgtgaa atatatgaag
    34741 tagctaatgg aaaatatgaa ttagagttgg attatccttt aggaactaga tttgatgaat
    34801 attttgaaaa tgactatcaa ataaaagcaa agccaaacga tcaagaagag tatcatatct
    34861 tttttattga tgataaagat atagatactt ttttaaatac agtaactatt tatgctcaga
    34921 gtcgtacaaa tcgacttgga agacgggtag tcactcttgc ggaagtagac tctaaaactg
    34981 gtcaagaagc gatatcaatt attgaaacca aaatggataa aaaatctgac atacgacttt
    35041 attctgatat tacggccgtt tctagcacaa tctttgaagc gagaaatgtt ttaaattgta
    35101 ttgctggtga acaaggatca ttgcttcaat attggggtgg agaaattaaa cgtgaaccat
    35161 ttaagctttc tttgttaaag cgaagaggtc gcaataatgt tggaacgatt cggtatggaa
    35221 aagatatgtc tggtttaaag gtcaaattag attggacagg tataaaaaca agacttattc
    35281 catatgctga tcctcaaagt gaggtaggta cgactaatcg aatttatggt tcgccagtag
    35341 atagtgccta tattaataac tatcctgatg tgtatacaga gcatgttcag tttacagaag
    35401 aacaaggagt aaaagacatt aagagcttaa ataaaatagc taaaaattac tttaaaacga
    35461 tcaatcctgg ctgtgataag cctaaagttt ctattacggt tgaatttgat aagttgactg
    35521 atagtgaaga agcgaaagaa tttgcgaaga ttagaaatta tggtctgttt gatacattta
    35581 aaatatatca taaaaaatat aatatttatc ttgaatcaaa ggttagtgga ctacaatacg
    35641 attctttatc tgaaaaaact ttgaaattag aagcaggaga cattcaagtt gctttttatc
    35701 agcaacaagc tgtaactatt caggataaat taaaagatta tgcaacgaat aattatatga
    35761 gtgattttaa tgattatgtt tcttcaatga ttacaggtca aggcagtgca ggaggatatg
    35821 taagtttatg gccaaaagaa aaaccttcca acatctttat tatggataat ccagatttag
    35881 aaaaggcaag agaagtacta agaatgaata aaaatgggat tgctttttct aagaaaggat
    35941 ggcgggggcc ttttacttca gcttggacat tagatagtat atttaatgcc aattttatac
    36001 aaactggatt aatcaaagca gatatctttc aaaattcttt taacaagact ggagatgtat
    36061 tgaaattagt taatggacta ctccaaattt ggaacaacaa aaagaaaata atggagttaa
    36121 ctaaaaaagg aatggaattt tggaattcta agggttcaat tggaacgatt ggaacaactg
    36181 attctgctgg taatcctttt cctggggctt ctactccaac ccctattgaa gataattctt
    36241 tagttattcg tacaaatgga gacggcaaat atattttgat ttctcctaaa gtaggaaaag
    36301 gattagtttt attaggaaat ggtaaagcaa tttattttgg agacttagat gtacaaggaa
    36361 aacttacagt taaaggaaaa gaaataattc ctggacaagg tggtggtcca tcgggaggtg
    36421 gagaatctac tggtgggtat cctagtgaat taaaaacaga tgcagaaaag agagcttgga
    36481 gaatttacga tattttgtgt aataacggat ttacgaaaca atctgcatgt ggaatattgg
    36541 ggaacattca acaagaaaca ggaggaactt ttgatcctga tactgttcaa ataggtggac
    36601 cagcatacgg attagttcag tgggatggtt cttcatatcc tcttgttggt ccagcaacat
    36661 gggatggaaa agtttatgtt caaaacttat ttaatgctgc aggtattaaa gaaccaataa
    36721 cgagcttaga tgcacaagtt cgattgctta tttggacatt tacaaatgga caatggatgg
    36781 gcgtagtaca acctacgacg gttgatggat ttaaggcctg tactgatcca agacaagcag
    36841 catatgcttt tgaacgaaac tatgaacgtc cggcagcgac acatcctgaa cgtcaagatt
    36901 atgcagttaa ctggtataac aaatttaaag atttaaaacc aggaggagct actggagaag
    36961 caggactaaa acatttagag tctttgattg gacaacgaat tggcaatggt cagtgttatg
    37021 gcttgtctgc agaatattca ggatatctgg gtggttgtgg catgggtgct ggaacaaaat
    37081 atggtttaac tcatgtgata ggaaatactt ctgcagcatc tgatattggt attgcctatg
    37141 attggtctgc tgttggttgg aaagtgattc aaaaccctag atatgatcaa ttagtagttg
    37201 gtgcaattat taattgggca agaggtggac aagtgggttc atggtttgca gacggaactt
    37261 atggacatac tggcgttatc cgaggcttag ctaatggtcg tatgcaaact tatgaacaaa
    37321 acacagagtt aggtatgatt tgtggaaagt tagatcgcca gtattatagt gcaagtgcaa
    37381 tttcttccat tgtcatacca ccaaaatagg gaggtgattt aatggcaaag tggaatgtca
    37441 tattaagtac aacagaaccc tataattacg tggggatgat tcaagttcga caaggcaata
    37501 aaaacactga agttatggaa gctacaattg tcgaaaatgg tcttccatat gatttatcag
    37561 aatgtaaggt atattttgaa tcagttgttg gcggaaaata tccagtccaa ttagaaacaa
    37621 gaattgtgga tgctaaaaaa gggaaaatca aatatatttt tgataaatat tccatgcagt
    37681 gtttgcatcg acaaacagcc aatttcatta tatttaaagg agaagacttg attggaacaa
    37741 ctcaagactt ctcctatttt gtcatcaatg ctgtttcaaa aacagaagga gaaatgggtt
    37801 cttattggca atcaattgaa gatttaattg ctgatatgac agattttatt aatgaaaata
    37861 aaggcgattt tacggactgg atgaatgaaa ggaaagaaga atttgatcgc tggagagaag
    37921 aacaggagaa tagtttccaa gattggagag aaggacaaga atcagattat ttgtcatggt
    37981 ttgaatcaat caaggatatt ttgaagtcta tcgatccagg tggagtaatg ttagccgaat
    38041 taatggatgc acgtgtagat cttcaaggtg tacgccatga atcgatttct gaacgtttcc
    38101 tatccgatct aaattatttg tatcaaaaaa tgaaagcagc acttttcacg attgaatacg
    38161 gtgaaattga agtgactgat attcttcaag acgatctctt ttcagataat cacgaagtcg
    38221 aaaaagttgg aactgtagaa ttcccgattg aagaaggagc cttgatcatc gcaaccgttg
    38281 atgatccaaa gcaaaatgtg ttcactcttg agaaagtcgg ggtgatctaa tggctaaaac
    38341 taaacgaatg atggaaacgg atgaaaaaac aggtgtacaa cgccaattct ttccaatcac
    38401 acatgcttcc gcagttcttg ggttagaaga aataatagca ggagaagcaa cagttttatc
    38461 tgttaatgga aaaattggag ccgtcgtcat tactaaagaa gatttgggat tagagaatgt
    38521 tctcacagaa ttaccctatg caagtgaaga agatgacggc attatcacgg cagaaatgta
    38581 tcaaaaaatt ttaaacagtg gagaaggtga ctacgtgttg ccagttgcca ctatcgaccg
    38641 tttgggtggc ataaaagttg gtgaattatt gacgattgat gagacaggga aagtctctgc
    38701 agtcagacaa tctgatgtca atttttcgct ggagttaaaa gaaaaactcg attcactgaa
    38761 aaattatact gctggagaaa acatcactat tgatgaagat ggaacaatta gttctacagg
    38821 tggtagtggg actggtggtg tcaatcagtc atatgtagat caaaaattcc aagaagctgt
    38881 aaatcaagca gaaaattaca caaacgaaag aattccaaat tttacttttg aaaaaattgg
    38941 ggaggtatag agaatgacag atatcgtaaa aataaaacaa agcaatgtac aggtttatcc
    39001 acaaactcat tggaatgcta tagaaggcaa accaacaacg gtgaagggag ataaaggcga
    39061 tcctggccaa gcagcaacaa ttaccatagg aaccgtttct agtggttcaa ccgcttctgt
    39121 tacgaatgta ggaacatcat ctgcagctag atttaatttt gttttaccaa agggagataa
    39181 aggtgatcct ggaataaatg ccacaactac agctgtagct acaactacag caaatgggtt
    39241 gatgtcgtcc acagataaaa ctaagttgga tggaattgca gctggagcac agaaaaatcc
    39301 aggtaatgct acaactacga cagcaggttt aatgtcagca accgataaag tgaaacttga
    39361 tggattagcc aatattacat ttgagaaagt ggggacggtt taatggctga tattgttcaa
    39421 ttaaaagaag acggagttgc taaatactta aaaacgcatg taaaagctat tgatggtaaa
    39481 gaagcattgg tgcagacaga tggagaccaa gccattgcag gacataagaa tttttcaggt
    39541 tctgtaacaa tcaataataa acgtgttttg acgactgatg ataacagata tgaagtggta
    39601 aatctggttg tcactaacgg taatacaggg acagcaaagc tttatcgtga aggaaaaaca
    39661 gtttctatat attttgtggc tttaaacgga aaaagtagtg gcgggaatga ttcggttatt
    39721 ttaactgttc cagaaggcta tcggacacca attagttttg aacaactggt tgggtcaata
    39781 gaccgttcta ctttgaacag tgctcaacta tctattggcg cagacggagc cattaaatgg
    39841 agaagaaatt caagttatgg atcagcttat tcatttgtta tcacttattc aatttaaggg
    39901 aggaaatcta atgaaagtag tttacaaatc aatcaagcct tacggattcg agcaaatcat
    39961 tttgaacaat caagaaaata tccctgaaaa ctgtacagag attaaaccac cagttcctaa
    40021 ctggagacca agatttgatt ttgataaaaa acagtgggtt gagttagcta ctgaagaaga
    40081 gaagagtggc acagcggttg acgatattga agatgtcgat cagttgaagc aattaaatgc
    40141 tctactgaca aaacaattgg cgatatcggt acaggaacaa gaaaaaatgc aacaaatgtt
    40201 agctcaattg acgatggaag tcgcaagtat taagaatgga gggaaaagta atgaatagtt
    40261 ttccgggctt tgaaaatata aaacaatttt atgattgggg atgttatacg gaccaagatt
    40321 tacttgacta tgtaaacatg aactgtttaa caaaagacca atataagcag attactggaa
    40381 atgaaattta attaagcaca aagttaaata aaaagcgtac tcaaacgagt gcgcttcttt
    40441 gcataaagga gaaatagata ttggaaaaat atttaaatac actctcagta atgacagggg
    40501 tgataggcgg gacaattgtt ggtttattgg gagggatgga caatatacta catgttttaa
    40561 tttttcttgt gggtgtggac ttcttaactg gacttgctaa ggcatggaaa ttgaaagaaa
    40621 taagtagtga gataggtttc gaaggattgt tgaagaaagt cttaatcttc gttgtgattg
    40681 ccgttgcggt tgacgtacag aaaatcgttg ggaattctat acctctaaga gaaatagtta
    40741 ttatgttcta tgttgcaaat gaagggatta gttttttaga aaacatttcg gtattccttc
    40801 ctttgccaga taagttaaaa gaggtattcc aacaaatacg aaatgatact gaaaataaag
    40861 accgaggagg aaccaaatga aaaagaaaat ctttgtagga gctattatag ctcttttttt
    40921 attgccaata aacgcctttg cttactcgat caacaatgaa ttcaatttgg gcgcaaacga
    40981 aggtagttct caagtagcaa ataaccaata catcctattg catgaaacag caaacgaaac
    41041 tgcgacagga cgaaatgaag cgcagtacat gaaacgttct tggtacaatg cctatacagc
    41101 gtatattgtt ggcgatggtg ggatcgttta ccaagttgga caacctggtt atgtacaata
    41161 tggcgctggt tcatatgcta atgcaaatag tcctgtgcaa atcgaactgc aacatacgca
    41221 cgataaagta acgtttgaaa agaactacaa ggcatatgtt gaattagcga gagattccgc
    41281 taataaatac ggtattccac ttacattaga cacaccgtat aatcaaccag gaatcaaatc
    41341 acatttatgg gtaactcaaa acatttgggg cgatcacact gatccgtatg gatacttatc
    41401 agaaatgggt gtaagtaagg aaaaactggc ttacgattta gctcatggat ttacagacga
    41461 aaacccaacg acttctgaga acaagcctgt cattgatcca acccgagcag gtgcagcaaa
    41521 tccaacttta tcagatggaa cgaaccattc tcacattgat cagtttggag aaatcgaaaa
    41581 tgcgaactta cacgtcgctg gatggcacat cgctaactat aaatacgagt atattttcat
    41641 tatggactac aatactggga aagaattagc tcgagtaaga gctgatggaa tttatagacc
    41701 ggatgtaaat caagcttata atacttctgg aaacgttggc tatcatgtat cttttaacat
    41761 gcgcaatttc cctaataaga aagtatacgt catgatgcgg gcaacgaatg atccagaagg
    41821 aaacactaaa ggcggtgcgc aagatttcca tgacaaacgt tggtatttaa atattcctaa
    41881 acgataaaaa tagctcctcg ttgaggagca gtacatagaa ttgaaaatta acgttaaatc
    41941 aaaaaaatat ttacaagatg tttatttaag tagataataa aagaaaaaag caggtgaaat
    42001 tgtgacagca gaaattggta ttatgaataa aagtggaatc gtgttagctt cagattcagc
    42061 atctacgatt ggagatagca aagtatataa tactgcaaaa aaattattta ctttggattc
    42121 catgcattct gtaggtatca tgatatatgg taatgctgaa tttaatggta ttccttggga
    42181 aataatcatt actcaatata agaaaagcat tggtagttct gtttttaata ctttggaaga
    42241 atacgcggat aattttatag aatttgtcaa aacagcatct tttattagaa gtgaacagac
    42301 cgagcaagaa caaatgattg gggtttttca aaaaataatt tcaggtttat ttgagagtat
    42361 agaagtagat attaactttt taataagcca aggtacacaa atagacaaag atgtcctagt
    42421 gaagttatta caaacaaaaa tgaataccaa tttgtctcaa cagtcccaga cattcatttt
    42481 agatattgaa aaggcgttat ttcttagtaa ctatggagaa atattaaaag atattcttaa
    42541 tagtatttct acaatggaag gtgtttcaga agctatatca gaggaaattc aaagttacgt
    42601 ttatgaaatt ataattagag acgatgttta ttcttcccct acaggaatag ttatagcagg
    42661 atatggaaga atggatattt tccctaaact atattcttat aatatgtttg gatttgttat
    42721 gaatattctg aaatactcag aatatgaaag tgctcaaata ggaaatgaca atggttcatt
    42781 aagatctact atattaccat ttgctcagtc tgatgtggta aatactgtag ttcaaggggt
    42841 agatccacaa attactaatt atctgtcgag tcaagtcgat agttttgacg ataacggaaa
    42901 aaatacctat attaatatta taaagaatat ttcagagttt caacaaaatc aattcatatt
    42961 tccgttacta aacatgattg ctttactccc agttgaagaa acagcaatta tcgcagagac
    43021 tttattaaat ttaaccagtt ttaaacgaaa atatactact tctgtagaaa cagtaggtgg
    43081 cccaattgat gttttagcta ttacacctaa tgatggccct atttggatta aaagaaaaca
    43141 ttattttgat attgataata atattggata cagattgcga aaggagagag cgaatgatta
    43201 caataattaa acctcaggaa aaatcccctg ttttgaaatt taatgagagc aatctagccc
    43261 ataataaaat gagtataact gatacagtat ttgatgtaat ggttgagcaa caaaaaaaaa
    43321 ttaataataa caatagacaa agaaaagaaa ttaaccaata ttaatgtttt ttctgttgcg
    43381 ttttttgcag tcataattta aaacctcgct caatcttggg cgaggtcttt ttttattttt
    43441 ctgaaaagtt aaagtaaata atagcataaa ataatatatt ttacaaagaa taagtacaat
    43501 ctagtttttt gctattaaat gtgtaataat aaatatgcca tcacaacatg aagaatgaaa
    43561 tccattatta tctagtctag tccattcttt ttgtttgcag tatttgtgat ggctttc
//
LOCUS       DACDYI010000015        43587 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.015, whole genome shotgun sequence.
ACCESSION   DACDYI010000015 DACDYI010000000
VERSION     DACDYI010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.015"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            157..837
                     /locus_tag="IWM52_000803"
     CDS             157..837
                     /locus_tag="IWM52_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP9973075.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            927..1961
                     /locus_tag="IWM52_000804"
     CDS             927..1961
                     /locus_tag="IWM52_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP9973076.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            1975..2310
                     /locus_tag="IWM52_000805"
     CDS             1975..2310
                     /locus_tag="IWM52_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973077.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            2294..4774
                     /locus_tag="IWM52_000806"
     CDS             2294..4774
                     /locus_tag="IWM52_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973078.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            4830..6389
                     /locus_tag="IWM52_000807"
     CDS             4830..6389
                     /locus_tag="IWM52_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9973079.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            6386..7750
                     /locus_tag="IWM52_000808"
     CDS             6386..7750
                     /locus_tag="IWM52_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9973080.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            7921..8991
                     /locus_tag="IWM52_000809"
     CDS             7921..8991
                     /locus_tag="IWM52_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9973081.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            9006..9674
                     /locus_tag="IWM52_000810"
     CDS             9006..9674
                     /locus_tag="IWM52_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9973082.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            9694..10272
                     /locus_tag="IWM52_000811"
     CDS             9694..10272
                     /locus_tag="IWM52_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9973083.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            10673..11584
                     /locus_tag="IWM52_000812"
     CDS             10673..11584
                     /locus_tag="IWM52_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9973084.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(11962..12879)
                     /locus_tag="IWM52_000813"
     CDS             complement(11962..12879)
                     /locus_tag="IWM52_000813"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAP9973085.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            13081..13518
                     /gene="zapA"
                     /locus_tag="IWM52_000814"
     CDS             13081..13518
                     /gene="zapA"
                     /locus_tag="IWM52_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAP9973086.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            13576..14124
                     /locus_tag="IWM52_000815"
     CDS             13576..14124
                     /locus_tag="IWM52_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAP9973087.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            14239..16599
                     /locus_tag="IWM52_000816"
     CDS             14239..16599
                     /locus_tag="IWM52_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP9973088.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            16686..17000
                     /gene="trxA"
                     /locus_tag="IWM52_000817"
     CDS             16686..17000
                     /gene="trxA"
                     /locus_tag="IWM52_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAP9973089.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            17182..18960
                     /gene="uvrC"
                     /locus_tag="IWM52_000818"
     CDS             17182..18960
                     /gene="uvrC"
                     /locus_tag="IWM52_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAP9973090.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            19105..20004
                     /locus_tag="IWM52_000819"
     CDS             19105..20004
                     /locus_tag="IWM52_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9973091.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            19997..21259
                     /locus_tag="IWM52_000820"
     CDS             19997..21259
                     /locus_tag="IWM52_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9973092.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            21420..23318
                     /locus_tag="IWM52_000821"
     CDS             21420..23318
                     /locus_tag="IWM52_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAP9973093.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(23648..24367)
                     /locus_tag="IWM52_000822"
     CDS             complement(23648..24367)
                     /locus_tag="IWM52_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAP9973094.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            24704..25036
                     /locus_tag="IWM52_000823"
     CDS             24704..25036
                     /locus_tag="IWM52_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973095.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            25023..27026
                     /locus_tag="IWM52_000824"
     CDS             25023..27026
                     /locus_tag="IWM52_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAP9973096.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            27045..27515
                     /locus_tag="IWM52_000825"
     CDS             27045..27515
                     /locus_tag="IWM52_000825"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAP9973097.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            27536..28120
                     /locus_tag="IWM52_000826"
     CDS             27536..28120
                     /locus_tag="IWM52_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAP9973098.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            28135..29121
                     /locus_tag="IWM52_000827"
     CDS             28135..29121
                     /locus_tag="IWM52_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAP9973099.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            29111..29422
                     /locus_tag="IWM52_000828"
     CDS             29111..29422
                     /locus_tag="IWM52_000828"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAP9973100.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            29444..31225
                     /locus_tag="IWM52_000829"
     CDS             29444..31225
                     /locus_tag="IWM52_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAP9973101.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            31218..32594
                     /locus_tag="IWM52_000830"
     CDS             31218..32594
                     /locus_tag="IWM52_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAP9973102.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            32601..33236
                     /locus_tag="IWM52_000831"
     CDS             32601..33236
                     /locus_tag="IWM52_000831"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAP9973103.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            33266..34621
                     /locus_tag="IWM52_000832"
     CDS             33266..34621
                     /locus_tag="IWM52_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAP9973104.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(34813..36207)
                     /locus_tag="IWM52_000833"
     CDS             complement(34813..36207)
                     /locus_tag="IWM52_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP9973105.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRSNQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            36299..37270
                     /locus_tag="IWM52_000834"
     CDS             36299..37270
                     /locus_tag="IWM52_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP9973106.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            37267..38049
                     /locus_tag="IWM52_000835"
     CDS             37267..38049
                     /locus_tag="IWM52_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAP9973107.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(38218..38589)
                     /locus_tag="IWM52_000836"
     CDS             complement(38218..38589)
                     /locus_tag="IWM52_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP9973108.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            38723..39166
                     /locus_tag="IWM52_000837"
     CDS             38723..39166
                     /locus_tag="IWM52_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP9973109.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(39209..40018)
                     /gene="yidA"
                     /locus_tag="IWM52_000838"
     CDS             complement(39209..40018)
                     /gene="yidA"
                     /locus_tag="IWM52_000838"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAP9973110.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(40037..41404)
                     /locus_tag="IWM52_000839"
     CDS             complement(40037..41404)
                     /locus_tag="IWM52_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9973111.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(41388..42218)
                     /locus_tag="IWM52_000840"
     CDS             complement(41388..42218)
                     /locus_tag="IWM52_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAP9973112.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            42381..42818
                     /locus_tag="IWM52_000841"
     CDS             42381..42818
                     /locus_tag="IWM52_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAP9973113.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            42894..43514
                     /locus_tag="IWM52_000842"
     CDS             42894..43514
                     /locus_tag="IWM52_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAP9973114.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
BASE COUNT        14278 a         7487 c         9251 g        12571 t
ORIGIN      
        1 ataaaaaagg aagaatattc atgtaaacga ttttattgtt tttttctctt ttatacaaaa
       61 agggtcataa tttcaagtaa gcggatgaat tcatgaggat tttttgcata ctgtctctac
      121 tatacttagg tcactaagaa aaaggagtga tttattatga aaaagaacat gattgggaca
      181 ttattacttt caacacttct tctaggcggc ttggcaacac cagcttttgc tgagggtcaa
      241 gcgacttcaa aaggagatat tacctttact gaaccaacaa ataccgtgga gcctcttaac
      301 cctactgatc cttcaaaacc agtagagcca gcagatccgg aaaatccagc aactggccag
      361 acaggtcctt taacactgga tgtagttccc gaattgccat ttggtacaca cgagattgaa
      421 agcggaacaa aaacgtatca agtagacgct tcaaaaaacg atacacctta tctccaagta
      481 tcagatcgtc gaggtgtagg tgcagatggc caagcacaag ggtggaacgt gacagtttcg
      541 gtttcagatt ttgtaaacgg ttcacaagtt ttgcaaggcg ctgaattaga ttttggaaca
      601 tccactgtta aaagtacgtc agataacgaa tccactggac caactagtca aactgtaact
      661 gggttgagta aagcaagtgc agcgacacca atatttacag cagcaaaaga tcaaggtctg
      721 ggaacatggc tttctgttta tgatccagca aatatcacac tgaaagtgcc gaaagcagca
      781 gctggaacat tcacggctga tttgacttgg aacctcgtgg ctggcccagt agcatagagt
      841 atgaaataaa ttcaacaaac tgcggtgaaa atactatagg tgaaggattt tttaccgcag
      901 ttgttgttga aaggatgtgg tggacaatga atcaaagtaa acgactgttt gtttcgtttt
      961 tttctatttt aagtctattt ttcatttttc cgtcaatcag ccaagcaaag gattcagcag
     1021 gagactttgg aatcaaaccg gtatttccag agaaccaaat agataaagca atcggttatt
     1081 ttgatttatt agtggcaccg gaacaaaatc agacactaga agtaattatc agcaattctt
     1141 cagatgaaga acgaacattt gaggtctctg tcaatccggc ggtaacgagt gatggtggaa
     1201 cgatcgatta tagtcaaaaa aacccaacgt tagatgagac attgccgttt gatgtccgtg
     1261 acgttttgct cattgcgaaa aaggaaatca atgtatcagc acatgcagaa acaactgtac
     1321 ctatagaagt gaaaatccca gcaaaaagct ttaagggacg tgtattagcg ggtattcatg
     1381 tttcacctaa agaagaagct gaaacagaaa atgctacaga aggagcacaa atcaaaaatc
     1441 gtattgctta taatctcgca gtcgttttac aagaaagtca agagacgata gaaccggatc
     1501 tgaaactttt atcaggagat ttggacgagg tcaacgcaaa gccaactgtt cagcttcgtt
     1561 tccaaaatcc tcagcctagg attatcagca atttgatatt tacgagcaag atcttttatg
     1621 aaaatcagct ttatattgaa aatacgtcta atgcattttt agttgcacca aattcgaatt
     1681 ttcacttgaa tcttgattta gctggtgata aagcaaaagc cggcgattac cgcgcagaaa
     1741 tcatagcaaa atcaggcgac agtaatgaat ggcgatttac acaaaacttt acgatcaaaa
     1801 aagaaaaagc acaaaaagta aatgaaaatt ccgtttttgc agtacaagaa caatctttcc
     1861 catggcttta tgcaggattg gttgctgcag ttctagtgtt atggttgatt ttgctgtttt
     1921 tgtgggtcca caagaaaaag aaaaaaggag aaacaacatg atgaggtgat ttcgttgaaa
     1981 acatcgatct tattgggagg gattattgca agtttgctgt tcttcagctg gcaagagcca
     2041 gtccaagcag ataccaaaag ttcaggtacg attacatttt atgatctaag tccaattccg
     2101 catccaagtg gttcagtcaa tggcgcatca aaacagcaga cacagctacc gatttataaa
     2161 gaatctaatc aacatggcgt attcccggag acgaatgaac aaaagatccg ttttctttat
     2221 ttgatcattg gtatagaatt atggcttctt gcgtggttgt tgttatggag aagaaaggaa
     2281 aaggaggatg aacatgttgc tacaaaataa attttcattt tttcgctcct tcttttttac
     2341 ttcgcttttt gttttaagta tatttatctt ttcaccttcg gcagaagctg ttggagcaac
     2401 gcctcctgcc atttcttatg atccatacac gtatgaaaat atcgtacctc caaatggtgg
     2461 acgtagtgca gtcaatggcg ggaagcttgt ccaactaact ggggctaacc aattgcctaa
     2521 attgactgct acttatttga acgatggttc aaatgatcct tggaaaggtg gcacggtccg
     2581 ttttcattgg gaaagccatg tgaaactaac aaatgtactt gatttggtga atatcactgc
     2641 acaaggattt gcatttgtgg cgatcatcaa tttgccggaa catgtatcaa gcagtgatgt
     2701 attaaaagca attgactgga acaacgccta cttgtatatt gccggtgccc ccatcaaggt
     2761 tactagcgga agtttattat cagcaggaag ccataccttg cgttttggga tggggtcgtg
     2821 ggatacaaat actctattga cagatattat tgctattatt acgcgaggcg aattcaacgt
     2881 ggacgatatc ccgcttcaat ttgatgttac agctaatgtc gcgaagatga cagaaaacgg
     2941 agataaatat gatacgagtc caaacaaaat tttgaccaag ggtaaattac aaccttctat
     3001 gtctaaaaag gccgtttttt ctgttgattt ttatgattcg aataatatta tccaagatcg
     3061 tatgttcaat tcgattttag ctccagctgc aggtagattt tatcctaaat taactggcgg
     3121 aaacttgaat ccaggagcat atgcattttc tgcaagtgca gagataaata cgtggaatag
     3181 ttacatctca ccaagtgatt caacaggaaa atataataca tttgctgata ccacagaata
     3241 cgtggatgga aataaccaga accttccagg ctctaccaat agtcggacat tgacgatggg
     3301 gctgactgaa ggacgttttg atcagttgcc atttagtcgc tttgatcgcg tagtcaacta
     3361 ttttacagga aaaaacgtta cacaaggagc aatactgact catactccag caagcacaag
     3421 tggattgaat cagacagcca atatcgtgta cagtgggaaa gatgcgcaag gcggattgct
     3481 ttctcctgtt gctttaaagg tattggagca gtacaagcta gacggaacgg ttttaccaac
     3541 aaacttaaca gccgatagtc agtggggaaa gctcctttct tctgcatcgt accaagtcac
     3601 ggccaattgg tcagcaactg aactaaaaaa gggttcgatc cattacaggc tttattcgaa
     3661 aggaacccaa aaattagcga caggatggac agatcaactt ttccaaacga tccaatcaaa
     3721 taacgggggt gtaaatacag ctacttctac actacctgca ttggcttcag ggcaatatta
     3781 ttttgattat cgcttggtgg acgatgtact tactaacatg tatccgtctt ttgcgtacaa
     3841 atggcagtct gaacaagctg gaatcaccac tcttcctcaa ataacagtgg ctaattttcc
     3901 atctgtgagt gcagatagtc agctaaaaaa ccttagcaga caacctgaga caggagatcc
     3961 cctgtctgct ctatcaggag atacgattca agaaaatgtg acattcacgt taacaaaatc
     4021 tggggatgcg ataaccgaca aaaaaatcac gatctcactt cctgaaaata ctacttatat
     4081 caatggttcc ttaaaattaa atggtacaac tatcgcagat accggcattc aacaaggagt
     4141 ttctgtccca gcagatctat tgagcaaaat aggagatacc attcatctta cttacaacta
     4201 tcaattaaat actgtagata cttctttaca atccgtcagc atactgacaa aggcagctgt
     4261 actttccagc aatattacat tagcagatgg tgctaagctt cctaacccag ttgtccaaac
     4321 atctgctaaa acaattcttg tccccaaaca agaattaaca ttagttaatg taccagatga
     4381 ttttacattt ggtaacgatc tccccaaacc gttgaagaca agttattatg aatcaaaagg
     4441 cgatttttct tttgatgtgc gggatactcg gcttccaagc acgtctcctt ggcaactgac
     4501 aggtacattg acttctttat ttaagaatga tcaaggtcaa gaattaagcg gaacaaaatt
     4561 atacttcaat cattctggat caaagcaatt gattcagcaa ggtcaaaaca cgttgattta
     4621 tgagagtgat ggaacagcta aaggagaggt tctcgttgac tttcccgata ccgatggttt
     4681 actcttagag gtaaatagca gtacgaatgc tcaacctggc gcaacgtatc aaggcatggt
     4741 cacatgggaa ctgacagctg ggccaaccag ctaagtgatt agaatttgta tggatctgtg
     4801 ataactgtca cagatcctct ttttcaagga tgagaaggaa acttctgctg ttaaaatcag
     4861 aaatgcttga aggagtggac ttggaacttt tcagtttgct tgaaaaaaat gaacaacggc
     4921 aaatcaaact tttccaactt ttattagagg aaaaaggaga ggtttccgtt aataacgtac
     4981 ttacccgctt atcagttgat cgggctacct tgaaagaaga tctgttttat ttacaagcta
     5041 acttggaaaa tatcaaacaa ttggaaattg ttatccaaga agggaatatt ttattaatca
     5101 gacatggaaa tatagcagca gtcgatgttt attatcattg ttacttgcgc tattctacaa
     5161 aatatcagat acttctttat ctgttggaaa atggctcatt tgatcggcag aaacttgtca
     5221 tggaacttaa tatcagttcg gctactttgt ctcgcagagt gaaggaatta aatgattctt
     5281 taaatgagtt tcatctgaaa ataaaaagcg ggcggctgac cggtccagaa agtcaaattc
     5341 gttattttta tttccaatta ttttggtttg gacgtcccta tcttgaaaac aaaaaagaat
     5401 acagtgattt atcaggaaac acttttttag atctgctgca aaaagagttt agttttgcta
     5461 ttacagaaga aggacgattg aaatttgaaa tatggctttc cattatgaag aagcgaatgc
     5521 gctgtacgaa aaaatccaaa ccagattata gcaatcttcc tgttggattg tttcaaacga
     5581 cacaattttt attggacctc catacatttt tgacccgtta cttctttcaa tatgcctttc
     5641 cttggaatca aaatgaaacg ttggtttttt atcttttcat gatcagtaac tttacattag
     5701 atacacataa ctcgcatgtc caggagtttt tgataagcga aaaatacaaa gacgacgtag
     5761 tagctcaatt gaatcgaacg tttaaacaat cgatagaaga atatttctct ttttatgttt
     5821 ttccagaaga gatttcgcaa aagattgcgt tgacagtgat gcaaaatcac gctcattttg
     5881 cctattttag aggatggatg aatatttttg gccaagaagg attaaaacag cggttaactg
     5941 aagtagaaga taaacaattc ctggttttat gtgaaagatt ggtcagacaa acatttgcta
     6001 ttgttgattt tactgaagta gacgaggaaa atgcccgctt tgaattattt gctcgctatg
     6061 catctatttt acggacgctg atcaagcagt tagaattacg tctgcaaatt gcttatgatc
     6121 ttccttatga aaggctaatg gcggatatcg tgaaagaaat catccaggaa caattgctga
     6181 atacgatcaa atatgattta gtcgaatacg agaaagaaaa gcagtatgat atcattttga
     6241 ctagccaatt gaaagagtac ccgtcacaga agtccgctaa agtttttgta ttcactagca
     6301 acgaatataa ttatgatttc ccctatttga accaattttt aaaagaatgt tatttagaaa
     6361 aattgaattt aaggatgaat aaaacatgaa aatcgtgatc attggtgctt cccatgaagg
     6421 catcacagct gccctaaatc taagaaaact tcaaccgaaa gcagaagttt tactgatcga
     6481 tgaagatcat aaagatggac taggctatgt ttcaaatgga atcaaccttt atttaaaagg
     6541 aaagatacgt agtttagctg aagttgccca taacatgcgg actcttcaag aaagtggggc
     6601 aaaactgatc accgaatgga gagtaactga acttgatcca gacaaacatc agctcatcct
     6661 cgcatccaaa gaaggaaaaa acgagaccat aacctatgat aaattaatcg tagcgacagg
     6721 ttcctctcct gtcaccttgt ataaacaaat tgaagctgaa aatgtctaca cctataaaaa
     6781 tctagtgcaa tctaagcaag tacttgccgc tttaaaagaa gcaaaagaag tagtgatctt
     6841 cggtgctggt tacattggat tggaattagc agatgctttg agaaataaag gatacattat
     6901 tcatctagtt gattatatgc cgaatgtttt atcaagatat ttcgataagg atatgatcaa
     6961 cagttttcaa aatcagctgc aaactaagca aataaatttt tatccaaatg aattcttgat
     7021 cgattggaaa aaatcagaag agaaagtagt atccgtacaa cttctgtctc aaacaataaa
     7081 ggcagatatg gtcatttttt ctgcccaaac tcgtcctaat acgacattgt taaaagaaaa
     7141 agtggctttg tatgaggatg agactgtgat agtcaatgaa tatctgcaga caagtgatcc
     7201 ggacatctat gcgattggag atatcgtccc agtcagtttt gacaaaaata aacggcatct
     7261 ctttttacct ttagttactc gagctgtaca tatggcaaga gcagtagcgc taactttgag
     7321 tggtcagcca accgcttacg acctcagaca aaagataaca gcagcagtca ttatagacta
     7381 ctttttagga actgtagggc tgacagaaga tgaagcgcca ttcttggaac aaaataccgg
     7441 ttcgtgttca ggagaatttg atctatttcc acaatacgac gaagaaaaca aaacagtaaa
     7501 tgcaaagctg atctaccatc cggatacatt agaaataatt ggcggacagc tgataagcca
     7561 agaattctta ttatcagatt tgaacctgct tgcggatatc gtgaaacaca aaactacgat
     7621 tccacagcta gcagttgagg attttggatt tctgtctgaa tacacctctc gttttcacta
     7681 cctcaatgag ttagctttta aagtactagc taagagagca aatagcaata gagtagataa
     7741 aagactgtga gaaatatcac ggtcttttat tgtgtgggga tataataaat attaatcaat
     7801 cgagaataaa aaattcatat aatgaaatag cggaagaatt ttttgagcat aatattcgcc
     7861 aaaaatttat caagtgataa aattgttgta taaaatgatt atttcaggag gaggagttaa
     7921 atgtttttag cattaaatga aattatgcat tcaaagttac gttatgcgtt agttgcaggg
     7981 gtgatgtttt tgatcgccta cctggtattt tttttgactg ggttagctta tggcttagct
     8041 caagataatc gaacagcagt cgacaagtgg gaggcagatt cgattgtgct ttcgaaagat
     8101 gcaaattcaa atctagggat gtccatgatt actaagaaga tagcagaaga agtcgaaggt
     8161 gggaaagtgg cgtatcttgc ccaaacacct ggagtcgtga ctagtaaaga cagtacagaa
     8221 gaaggaaaaa tcaatgttag cttcttcggt atcgataaaa atcaattcat catgcccaat
     8281 cttgttgaag gaaaagcttt tgataacgat gatgaggcag taggcgatat tagtttgaaa
     8341 gaagaatatg gtctagccat aggagataca gttaagttat ctggtagtga caagacgttt
     8401 aaactaactg gctttacgga tcatgccaaa ttcaatgtct caccagttct ctatacaacc
     8461 atcaatgctt accaagaaat ccgcttcgaa aaagaagata caagtgaaaa tgcacggatt
     8521 aacgcaatag ttgtcagagg taaaatcagt aatttacctg aagatttaga acagatcaaa
     8581 atttctaaat tcatcaatga attacctgga tataatgcgc aagtattgac ttttggattt
     8641 atgatcggat ttttgatcgt tattgcggct attgtgatcg ggattttcat ttatgtactg
     8701 acgatgcaaa agattaatat ctttggtgta atgaaagcac aagggatcac aggaggattt
     8761 attgcgcgtt ctgtagtggc acaaacattc attttatcat ttgttggtat tcttcttggt
     8821 ctattaggaa cagtgggaac atcccttgtt ctacctgatg cggttccatt ccaaagcaat
     8881 tggctgtttt ttggcgtcat tagcttgttg atgttagtag tagctgtgtt aggcgcacta
     8941 ttttctgttc ggacaatcgt aaaaatcgat ccgttgaagg caattggata agaaggagga
     9001 agaacatgaa agcaatagaa tttaaagaag tagaaaaaag tttccaagat ggcgaccaag
     9061 tagttaaagc attgaaagaa acaaccatga gtatcgataa aggggaattt gttgcaatca
     9121 tcggtccttc tggttcaggt aaaagtactt ttttgacttt ggcaggcgga ttgcagacgc
     9181 ctagcagtgg ctctgtattt atcaatgatc aagaattcag taatcaaaaa gaaagcgaac
     9241 gcgttaaact acgttttaaa gaaattggct ttattttgca agcttccaat ctggttcctt
     9301 ttctgacagt aaaaaagcag ttattattag tagataagat taaacatgaa catcgtcaaa
     9361 aagaagcaaa cgaattattt gagcagcttg gcgtggataa gttgatcaac aagtatccag
     9421 aagaactatc tggtggagaa cggcaaaggg tcgcaatcgc tcgtgcattg tacaatgatc
     9481 cgtccattat tctagcagat gaaccaactg ctagtttgga ttcagaaaag gcacgagaag
     9541 tagtcgatat cttggcaaaa gaatcaaaag acaaaaataa agcaacgatc atggttactc
     9601 acgatacacg gctgattgac aaatgcgata gaatatttat gatggaagat ggtgtattga
     9661 aagaaaaaga ataaccaaaa agcaggtgga aaaatggtcc atcgatacaa caaagataca
     9721 cagcaaaaaa ttttgaaaga agcgactgac ttgtttatgt ccaagggata tctcggcact
     9781 tcaacaagag aaatcgcaca aaaagttggg atcacccagc ccaatctcta tcattatttc
     9841 ggtgataaag aaaaactata tacggcagtt ttagaaaacc acttaaaaga tgttggaaaa
     9901 gctttgagag aaatcgttca aacaagcgaa acgggatttc aagtaacctt gacaaaaatg
     9961 gcgcagtttt tgattgagac acatctggtc gatttgtttc tgatgcttca cgatcttgaa
    10021 agcaatcttt caaaggaaac aagagatcat cttttctttc tatggaagaa aaattatcgt
    10081 gaaccttttg aagaaatctt ttcagcaaac caatctgtgc taagaaatgg gatttcaaaa
    10141 gaaatcgcag caaggcattt tttcttagtg ttagcacctt acatcactcg gtcgaaaaga
    10201 cctgtggaag aaccgctaaa tgtggcgcaa ttgatcgatt tatatttgaa tggtgtatta
    10261 ggagaagcgt aaagctgtta tatagggaga aaatagttag gtgaagaagc aagatatgac
    10321 gaagatcgtt atatcttgct tctttatata ggtagttttt cataaataca aatttgttat
    10381 tctctcatga ttatcagggt atatttttat ttattatgtc ggtgttccag gaagtgtttt
    10441 aatttaataa cgaagaaatt aattgtttgt agaatgaagg aaactattta ttgggtagat
    10501 gaatcaccac gaatagtcaa aaggagattt caggatatgc caaaaatgaa aaaaatcatt
    10561 ttagcagtaa tgttattttc tacggttata tcttttacag cccctttttt gcttacttaa
    10621 gctgatttta agttttggcc cttagtctgg atctacgagg tgagagtcaa gaatgaaaaa
    10681 aataattgga gcttttgtat ttgcaatcat tatcattatt atctgttttc aaatagatgt
    10741 ttttggtcaa aaaaagatag ttggacaaac ggtcagcagt acgaacagta cggttacaaa
    10801 gacaaaggaa tcatttgatg atacagaaca agagacatta atcaaaatcg gaaaaaatga
    10861 ccgtacacta tatacagatg actcgctaac taccccactt gcaactataa acggcggaga
    10921 gctggcggag tttctttctg aaacaaaaaa tgcttatcag atcaagacaa atgatggcta
    10981 tactggatat ttagccctcg aagatggaac caaagtcaca aagaatatac aaacaaaacc
    11041 ggaagagcta tctgatgcag tgattgtatt agatcctggg catggcggaa atgatactgg
    11101 ggcattaagc aatgatgaac aaacagaaga aaaagatata acattaagta cagccataaa
    11161 agtaaaaaag gcgctagagg atgctggagc aacggtttat ttgacccata ctacagatga
    11221 attagtacag cttggagata tatgcgacta cagtgaagaa aaaaaggcag atgtctttat
    11281 cagtttacac gcagactcta cggaatatgc gaatgaagca actggaatca cgacatatta
    11341 ttactatgga caagaggaaa cattagcaca gacaattgct gactccttca ctgatctgcc
    11401 tctggattca agaggaatct ccactgggaa ctatcaagtt cttcgagaaa atcttcaacc
    11461 ttctatttta atcgagatgg gttatatgaa taatgatagt gatttaaagg aattagtaac
    11521 agacagttat cagcagcaga ttgctgcaag tttgacacaa gcattgacca cttattttca
    11581 gtaataaata agcccagcca aacaaaaata gctcctcata tttttcgttt ggccgggctt
    11641 attatcgcag atgcaagctt ttaaacaccg tttaaggaag aggtcgtgag cctgccgttc
    11701 aactctgagt cacaaggagc aatttcaaaa aacagcttct catgtttgat gactattgtg
    11761 acttgtgacc gaggagttgg catgagaaca ccgtttaaga aaaaggttgt gaaatcaacg
    11821 ttttgttctg agtattagag aaacaaacgg aaaaatagtt cctcatattt tgccgtttgt
    11881 tgagctaata ccgaggatca gatgattgaa caccgtttat tcggaacaag ggactgtgac
    11941 atgacttttg tcacagttcc attatttttt tagtagcttc aaagcttttt ctgtattggc
    12001 aaaatgaagt ttggcatatt tttcaagcat aggcatgccg cctttctcca atagttttgc
    12061 cgctacttgg tcagatttcg ttccagctcc tgacggaagg gtgattccag cttcagctga
    12121 tgctttgtct aactcttcca aaaagctggc acgactgatg atagaagcag cagcaacagc
    12181 taaatgatat tgttctcctt tagtaacgaa gtacagtttt tcttgtaatt gatttttttc
    12241 taaacgcgca tattttttaa aatttgcttc cggcgtaaac tgatcgatca aaatcgcttc
    12301 tggtttttct ggctcgattt tttttagcag caaatggatc gcttgattgt gcaaagcgac
    12361 tttcatccga acggcattgt attttggttg gatctcattg tatttttctg gagataagac
    12421 aagcagttta taggggatgg ttttttttaa gatgtcagca agctggatga tttggctatc
    12481 ttttaactct tttgaatctc taacaccaag tttacgtaat tctgcaagct gatcacgttt
    12541 cacataagca gcacatactg ttagcggacc gaaatagctg ccatttccga cctcatcact
    12601 acctaaaacg gataattgac tgatattttt tggcaaagaa gaggctgttt ttgctggctt
    12661 ggcttgttta tcctgtgcct ccccccattt atcagcttct attgctgcat tcgtaccttg
    12721 aaacatcact tttccggatg tataagcggt aatggtcgtc ccgtcttttt ttgcagcaaa
    12781 gctagtataa ggaatctgtt tatttaacaa ataagagcga tagtatgctt tcatttgact
    12841 aagttggtcg tttgttgctt tgataatgac gttgctcata aaattctcct cgtttttata
    12901 cataaaacta taccgcaact cacgactttt cgttttctaa gagattgcga gacatagcat
    12961 ttttttaaag tttatcatat aatagttaag tatgtgaaaa atatttgtgg agaacggtga
    13021 aagaaccaac ttcatggtac actaaataaa actttcatca aataggagga aatgattcgt
    13081 atggcgcacg aaaaaacacg ctacaaagcg gtgattgcca accaaacata tactattatc
    13141 ggacgggaaa ccaagcatca tatggatatc gtaacgaagc tgatcaatga acagttagca
    13201 gaattgaaac aattatcacc acaaatggat aatgaacaag cagcaatctt gatggctgtc
    13261 aatgcgcttt cggatcaatt gaagaaacaa gaaagaatat tggagctgga agaagaaaca
    13321 gcagaattaa agaaaaaaat gatcaaattt acagagcttg aaaatcgtgt gaaacgcatt
    13381 gaagcaatcg agaacgaagc tcgggaagta ttgaaagaaa atggtcaagc agatcatacc
    13441 attaaaaatc atgtggaagc acaacagatt ttaaacgaga aacgaaaagg acaaattaaa
    13501 caaaaatctt ctaattaaga tccttttatc aaaataagaa aaatttaaaa aagttagaag
    13561 agaaaggaac gtagcatgct gagtttgctg attttatttt tattgctaat tgcgtttttt
    13621 tctggagcac gtcgcgggtt tgctttgcaa atcatattcg cgattggata tgtattgtca
    13681 tttattgctg cccagcattt ttacaaacca ttagcaagtc atttggaatt atacattcca
    13741 tacccagctg tcaccccaac atcaaagtta gcattttttg atcaggtatt tgcctttcac
    13801 ctagatgaag ctttttatgc aggaactgct tttctgatta ttttgctaat tggctggctg
    13861 cttacccgat ttgtgggggt ttttgtccat ggattgacgt atgtgccgat cttgagacag
    13921 gtagactgga ttgcaggagg catcctgtca ctaatcatgg cttacgttac tatatttttg
    13981 attttgcagt tacttgcctt tgttccatta gatgtggtcc aaaatcaatt cggcgagcat
    14041 agtttcgctc gttttatcgt tgagagaacg ccatttctta ccaacaagat gtatgagctt
    14101 tgggtaacga atattttgaa ttaaggaaaa tagtgctgga aaatatttcg ttttttgtac
    14161 agagtagttt atttaagaag gctgcgtagc gtatgtccgg cacagctctt ttttcatagt
    14221 gaaagataag gtgataaaat gaataaccga atccttgaaa cattggaatt tgaaaaagtc
    14281 aaacagatgg tcagacaatt tgtagtgact gcacaaggaa aagaagaact ggcagaattg
    14341 gtaccagtga gtgagaaaca aacgatcaca aactggcttc aagaaactga ggatggattg
    14401 aaagtgcaac gtctgcgagg cgggatccct atacctaaaa ttgaaaatat ccgtccccat
    14461 atgaaacgga ttgagatcgg agcagacttg aatggagtgg agcttgcaca agtttccaga
    14521 gtattgtcta cgacaagcga attgaatcgt ttcattgatg atctttctga cagtgaaatc
    14581 gaatttgcac gtttgtatat gtgggcagat cagttagtca cgatccctgt tttgagccgc
    14641 cgtttaaagg aagcaatcga tgaagacgga cgcgtgactg atgacgcttc tcctgagtta
    14701 aagagcataa gacagaacat tcggcgaagt gaacaagctg ttcgtgaaca gttagatggg
    14761 atcgtccgtg ggaaaaatgc aaagtattta agtgatgcga tcatcaccat gcgaaatgac
    14821 cgctacgtta tcccagtcaa acaagaatac cgcggtgtat ttggcggtgt agtccacgat
    14881 caaagtgctt caggtcagac tttatttatt gaaccaaaac aagtggtgga tttgaataat
    14941 cgtttgcgtc aataccaaat cgctgaacga aatgagattc aacgtatttt aagtgaactt
    15001 tctgctgaac ttgttcccca tcgtcaagaa atcatccaca atacttatgt gattggtaaa
    15061 atggatctga tgaatgcaaa agcacgattt ggtaaagaag taaaagccat cgtgccggga
    15121 atcagtgaag acaatcatgt cgtcttgaaa caagcaagac atccactgat cgatcaggaa
    15181 aaagtagttt ctaatgacat cacaatcgga aaagactatc aagccatcgt cattacagga
    15241 cctaacacag gtgggaaaac gatcacgttg aagactttag gtctgttaca actgatgggg
    15301 caagcaggat taccgattcc tgcaggtgaa gaaagtcaaa tcggtatttt tgaggaagta
    15361 tttgcggata taggagacga acagtcgatt gagcaaagtc tatcgacctt ttcttctcat
    15421 atgacgaaca ctgtagatat tttatcaaaa gtcaatgaaa aaagtctggt attatttgac
    15481 gagctcggtg caggaacaga tccgcaagaa ggtgctgctc tagcaatcgc aatcttagat
    15541 gatcttggta aaaagtctgc ttatgtgatg gcaacaacgc attatcctga attgaaagtt
    15601 tatggatata atcgggcaaa tacgattaat gccagcatgg agtttgatgt ggatacatta
    15661 agtccaacgt atcgtttgct gattggcgtt cctggacgaa gcaatgcgtt tgaaatctct
    15721 agccgattag gtcttgatac agaagttatt gatgaagcga agcagttgat gaatgatgag
    15781 agccaagatt taaacgaaat gatcacagat ttagagaatc gccggaaaat ggcagagacc
    15841 gaatatctgg aaatgcgtca ttttgtttct gaggcacaag aacttcacga tgatttgaaa
    15901 gaagcttaca gctatttctt tgaagaacgt gaaaaagaaa tggaaaaagc aaagaaaaaa
    15961 gcaaatgaag tcgtgtcaga ggcggaagaa aaagcagaaa aaatcattgc tgatattcgt
    16021 aaaatgcaac agcagattgg gcaaggaaat gtgaaagagc atcagttgat cgatgcaaag
    16081 acccaattag caaatctaca tcaagaagaa actttgaaga aaaataaagt attgaagaag
    16141 gcgaaagaac aaaaaacgtt gaaaccggga gacgaagtgt tggttacgac ttacggccaa
    16201 agagggacgc ttttacgtaa aaacgggaat cagtggcaag tagaaatcgg tatattaaaa
    16261 atgaatgtgt ccgaagacga gctgacgcct gttgctcctc aaaaagagcc gacacaacga
    16321 gttatccatg ctgtacgatc tgaatctagt agtcacgtgc cgaatcaatt ggaccttcga
    16381 ggaaaacgat acgaagaagc gctaagtgag gtagatcaat atttggattc tgccattcta
    16441 gctggttatc ctcaagtgac tattgtccac ggaaaaggaa caggcgcact tcgcaaggga
    16501 attactgatt atcttaaaaa ccatcggagc gtcaaaagct ttgagtttgc accggcaaac
    16561 caaggcggaa atggtgcgac aattgttaaa tttaagtaaa gcttactaaa ggtaagcaga
    16621 tggttcgcta tccaagtata gaaatgctat actagtcata gattttaaaa atggaggtct
    16681 ttgatatgtc acaagtaatt acagataaga catttaatga agaaacagat aaaggacttg
    16741 tattgattga tttttgggca acttggtgtg gtccatgtcg tatgcaagca ccgattttgg
    16801 atcagttgga acaagaatat gatgaagaag aattcagaat tgctaaaatg gatgtggatg
    16861 aaaatccaga aactcctcaa caattcggta tcatgagcat tccaacatta atgttgaaaa
    16921 aagatggtca agtagtagaa aaagctgttg gtgttcattc aaaagaacaa ctacgtcaaa
    16981 tgatcgatca atatttataa aaacttacag caaaaataag ctgagggtgg gactgaatcc
    17041 agttccgtcc tcggtttttt tgcggttgag ttaaggttat tcaaattaat attttttgag
    17101 tataattacg ttgtcaggct ggattgtttt tttgacagac atgctataat aactttaatt
    17161 gaatatgaag gggagtacca gatgaacgaa cgaatcaaaa acaaattagc tttacttcct
    17221 gatcagcccg gctgttactt gatgaaagat aaaaatggaa cgatcattta tgtcggcaaa
    17281 gccaaaatat taaaaaatcg tgtccgctca tattttactg gtagccataa tacgaaaacg
    17341 gaacggcttg ttagtgaaat cgaagacttt gaatacatcg ttacagaatc gaatatcgaa
    17401 gcattgctct tagagatcaa tctgataaag aaaaatgatc ctaaatacaa cattatgtta
    17461 aaggatgata aaacctatcc tttccttaag ataacgaatg aaaaatatcc ccgtttggtg
    17521 attactcgta aggtactaaa agacaaagca ctgtattttg gaccatatcc cgatgtcggt
    17581 gcggcgaatg agacaaagaa gatcctcgat cgattatttc ctctgagaaa atgcaaacct
    17641 agtcagacga aggagccttg tctttactat catttgggac aatgcctgtg tccttattat
    17701 ttcgacgtag atccgaaagt ctatacgaaa atcgttgatg aagtgaaaaa ctttttaaac
    17761 ggaggacacg aaaaaatcga gtcagagatc catcagaaaa tggaaaaagc tgctgaaaac
    17821 atggagtttg aaaaagctgc agaataccga gaccagatcc gtgccattga gacaatcatg
    17881 actcgccaaa aaatgacaaa tactgattta ctggatcgag atgtatttgg ttatgcgatt
    17941 gataaaggat ggatgtgtgt ccaagtgttt ttcgtaagac aaggaaagct aattgaacga
    18001 gatgtttcaa tgtttccttt ctataacgaa gcagaagaag atttcttgac ttacctcggt
    18061 cagttttatc aggaaaatga gcatttcatt cctaaagaag tcttgattcc tgatacgatc
    18121 gacaagccca gtgtagaagc tttgttagct acgaaagtgc tgcaaccaaa acgaggagaa
    18181 aagaaaaaat tagttgaatt ggcgagtaag aatgcctcag ttgctttgaa tgaacggttt
    18241 gatctgattg ccagaaaaca ggaacggacg atcggagcag ttgaacgttt aggaaacgcg
    18301 atgaatatcc cgaccccaat acgtatcgag tcttttgata actctaatat catggggacg
    18361 aatcctgtgg ctgcgatggt cgtattcatt gatggaaaac cggcaagaaa agaatatcgg
    18421 aaatataaaa taaaaacagt caacggaccg gatgattatg catcaatgag agaagtcatc
    18481 tatcgcagat attcgcgagt gattcgcgaa gggttgcctt tacctgattt gatcttgatc
    18541 gatggaggaa aaggacaagt agatgtggca aaagatgtct tagaaaatca attaggaatc
    18601 gatattccta ttgctggcat ggtaaaaaac gataaacata aaacaaatga gttgctattc
    18661 ggtacagatt tggctgtagt accgcttgag cgaaattcac cagagttttt cttactccag
    18721 cgaatccaag atgaagtcca tcgattcgca atcactttcc atcggcagct acgcagtaaa
    18781 aacagttttg cttcccgatt agatgaaatt gaagggttag gaccaaaacg aaaaaaaatg
    18841 ctgcttaaag agttcaaatc gttgaaaaat atcacagcag ccacgacaga agaattacaa
    18901 gaaatcggac tgccgaaaaa tgtcgcacaa aacgtatttg ataaattaca tcaggaatga
    18961 ggaaagggca ctttacaaag aaagtaaagt gtcctatcat tttatgtaca acaaaaagag
    19021 cgtttacgtt ttgttggtaa ataataaatc gcttacgtct ggtgtctaag gatatatgtg
    19081 atttcttgaa gaaggagtga agaaatgtta gaagtcaagg atcttgttaa aacttttggg
    19141 aactacacag ctgtcgatca tgtctctttc caaatccccg atggaaaaat catggggcta
    19201 attggccaaa acggggctgg gaagacaacg acttttcgtt tgatcttgga ttttttgaca
    19261 caagaccaag gagaagtgtt atggaatggc catcatcttg gggagaaaga ttatgatgtc
    19321 atcggttatc ttccggagga acggggactt tatccgaaag taacgattca agaccaactg
    19381 ctttattttg cagaacttag agggaaatca agaaaagaga ttgaaccaaa aatcgacttt
    19441 tggatggaaa aatttcaggt aaaagggaaa aagacggata aagtaaaatc tttgtctaaa
    19501 ggaaatcagc aaaaagtcca gctgatcgcc acgttaatcc atgaacctaa attgatcatt
    19561 ttggatgagc cattcagcgg gttagatccg gtaaatgctg aattgctaaa agatggaata
    19621 ttggaattga aaaaacatgg ttcatgtgtc attttttcta gccacaatat ggataatgta
    19681 gaaaaaatct gcgatcattt gatcatgttg cgaaacggaa aaatggtttt gaatggaaaa
    19741 gtacatgaga ttcgtgaatc atttggccgt acgaagttat tcttggaatc tggtttaagc
    19801 caacaggaag tagaagaaat cgaaggcgtg aaaaaagtga tccttcatga agacaatacg
    19861 ttagagatta ccttagaaga tcctgaagct gggaaagaaa tctttactcg tgcgactcag
    19921 ttcggctaca ttcctatgtt taaccagcaa ccgccaactt tggaagagat tttcaaattg
    19981 aaagcaggtg aaatcgatgc gtaagttttg gattattacc aaagatgtct atttaaaaaa
    20041 tgttaagtcg atttctttct tgatcatgat attagtccct tttatcatgc tgggtatcat
    20101 ttatgtagca ggaaactttg ctcaacaaaa tagtgatgtc aataaaatcg gcgtattggc
    20161 tgataatgag caaatcagtc agcaaatgag ccaagtcaaa acagatgatt ttgagttcaa
    20221 agcagttgat tctgaagaaa aagcacaaaa agagctttcg gaagaaaaaa tcgatgcgta
    20281 tttagtagtc tcaactgaga atgataagat aaatgggatg ctttatagcg aaaattcatt
    20341 aggacaaagt acacaactac tgatacaaca acaactgact ggcttgcaat cgatgcttcg
    20401 agcgagtcaa ttaggcattt caccagaaga agtagctagc ttgagccaac aagcaagttt
    20461 gactcgacaa aaagtcaatt ttgattcaaa aggaaaaatg acagtcggag aagataactc
    20521 gacagtccaa tacgtcgtaa gttatgtagc aacgattgtc ttgttcatta ttattttgac
    20581 gtatgcgcag atcattgcac aagagatcgc ttctgaaaaa ggcacgcgga tcatggaagt
    20641 gatcctgtcc agtacgactg cacaaaagca tttttacgga aaattaacag gtgttttact
    20701 tgttgcatta acgcaaatgg cattatacgg tattattttt ggtataggat acagtcaatt
    20761 caaagatatg gaaatggtca aaaatatatt agacggtatc tcattggata acttgattgg
    20821 accattcttg tggtattctc ttctattcat gatctttggc attttgattt tttctgtttt
    20881 agcagcttta tgtggctctt tggtcaacaa agcagaagat acagcaaaag caattttgcc
    20941 agtgacttat ctatctctag gcggatacat gcttggattg atactaggag cttcagatcc
    21001 aaacaatatc gttattcgga tcacttcgta tattccattc ttatcttcat atattatgcc
    21061 gattcgctta gcaaatgaga cagtcggtat gggtggagca ttgatttcct tgttggtact
    21121 tgtagccgta acattcttat tgatgatctt gtctgcaaac atgtacaaat cgaatgtact
    21181 cgtttatagc gaaggcggat tatggagttc attgaaacaa tcgatttcaa ttatgaggaa
    21241 tgaacgaaaa aaagcataaa acaatcctaa aatacagaag gaagctggac atctattgtt
    21301 cccagcttct ttttgtatag acctatttta tttattttac tatattggat atatattcat
    21361 tgaaaatagt gtataacaac tttataatat tgagtatacc aatttttgga ggcgttttaa
    21421 tggaccgtta tttagattta ctttcagaaa aattcccctc aacggaggat gtcatcacgg
    21481 aaatcatcaa tctcgaagca attttgcagc ttccaaaagg aacagagtta tttttgagtg
    21541 atatacatgg cgaatttcct gcttttgatc atattctgcg tatcggttca ggaaatctta
    21601 aagaaaagat caaagacctt ttttctgaac aaatgacgga aacggatatc agccaattca
    21661 ctatttttac ggcttatcca gaatatgcat taacgactga ttggtacaaa gaacaagata
    21721 aatcgcagct gatccatcat ttgatcgatc ttttacggtt tacgacagtc aaatataccc
    21781 ggtcaaaagt tcgaaaagga ttgccgaaag aatatagcta tatcattgag gaactcttgt
    21841 atatcgatga ccgtatcaat ggaaaaaaag actatgtaaa aaaaatcatt gaacaacttg
    21901 ttttgatgaa ggaagaagaa aaatttttaa cgaaacttgc acagacgatc caaaaatctg
    21961 tcatcgatca tttgcatatc gttggagata tttttgaccg aggaactcaa gcagccaaag
    22021 tgatggatcg aatcatggat ttttcttctg tagatatcca atggggaaat catgatattt
    22081 tgtggattgg tgcgtattgc ggctctgagg catgtttact gacactttta cggattgcag
    22141 cacgctataa ttatttattt gaattagaga aagaatatgg gttgaattta cgtccattgt
    22201 tttcttttgc tcatcaaact tatcagaaga atccagtatt tgcgcctaaa aacagtaaaa
    22261 atctatccga aagagagcaa gaagaattag aaaaaatcca tcaggcactt tctatccttc
    22321 aattcaaatc agaacatcag ttattggatc gtcgacctga atttaaaatg gacgatcgta
    22381 aattgctgga aaaaatcgac tatgaagcat caacgattga tttagaaggt cagctctatg
    22441 agttgaaagg aacttgtttt caaacgatcc agccagatga tccaaaaaaa ttcttggaag
    22501 aggaacaaaa agtgattgat agtttgatgt attcattcca gcactctttg agaatgcaaa
    22561 aacatatgac gtttttgatc gaaaaagggg gaatgtatct cacgaacaat cagcatgtgc
    22621 tgtttcatgg ctgtatacct gtagacgaga agggacagct cttagcattt gaattagacc
    22681 aatcttatcg cggaaaagaa ctgctgggct tttttgaaaa acagataact gaaagcgcaa
    22741 aagatctgac tgcaaaagaa gatttagcaa ccgatttgat ttggtatgcg tggagtgggc
    22801 cattttcgcc tttattcggc aagagtaaaa tggcgacttt tgaacgttat tttctcacag
    22861 agcctcatac gcacgtcgag aaaagcaatc cttattatca cttgcgagat gaagagtggt
    22921 tcagtgaaaa aattttagca gaattcgaca caaaagaaga agggtcagca atcattaatg
    22981 ggcacacgcc agtaaaagta aaaaaagggg agtcaccgat caaagcaaat ggaaaagtat
    23041 ttgtcattga cggaggttta tccaaagctt atcaaaaaac gacaggaatt gccggttatt
    23101 cactattatg caattcttat ggtttccaaa tcgtgacaca ttatccgttt ttgcctattg
    23161 aacaacaatt tgcaaaaaaa tccgaccaaa caaatgtgaa aaaagtaatc gaacagcaac
    23221 taccacgcaa actcaatcgt gatacaacaa aaggtatgga actgcagcag cagattaccc
    23281 agctcaaaag gctgttggac tatcgaaagg acgattgaga agaattccag tgaacattgt
    23341 tccatagact ggagactgag acaaaagtcg tgtcgcagtc tctttttccg gataaacggt
    23401 gttcttctgc caactcctcg gtcacaagtc acactattca acaaacatga aaagctgttt
    23461 tttgaaattg ctccttgtga ctcagagttg aacggcaggc tcacgacctc ttggtataaa
    23521 cggtgttcaa aagccagttt ctcggtatta agccaaattt atgcaaaaat gtgaaaagcc
    23581 attttcacaa aatttttctt aatactcgaa actaaacagc tttttcacaa ccccctcaga
    23641 aagtctatta aaattcattc acaaagaaat cgaataagcg ctgttcagat ggatgtacac
    23701 ggtgggtaag ttccggatgc cattgaacac cgaggatttt atgcatatca tctttgcttt
    23761 gaaccgcttc aacgagtcca tcgttagaaa aagcgatggc ttttaataaa ggtgaaagat
    23821 ctttgactgc ctgatgatgg taagaattca caaaatatgg atcggggaga aggttgctta
    23881 acaaactgtc tgaaacaatc ttgacttcgt gagtcgtaaa ttgcggggca gtgggctgtt
    23941 gttcatgttt tactgaccac tttggatata atgaaagatc ttgataaagt gtaccttcca
    24001 aaacgacatt caatagttgg agtccccggc agactgcaaa aattggtttt ctttgtttca
    24061 atgcttccaa gatcagtgcc tgttcaaatt ggtctcgttg aatatttgtt tccgttagct
    24121 ttggatgagg atcttccatg taaagttgtg gactgacatc ttgaccacct gcaagaataa
    24181 gtttgtcgat ttgtccaatc agttgaggag ctgaatctgg agacatgact ggcagaataa
    24241 gaggcaatcc ttgagcatct aaaactgcat ccacgaatcc ttgcggtgta tagctcactt
    24301 gatttccttg gaaagtatct gttgctcgaa tcagttgatt tccggcaatg ccgatgattg
    24361 gtttcatttg tattccccct aaagtagact tattgtaaca aggatttaaa aaaaattaaa
    24421 ctatgtaaga cttgccccta tacttatctg ttcttgctgt gttactatct gtaaagagaa
    24481 atttcgtgtg aaattcatga cgatagttga aaatattgct taatttgtta taataaaaag
    24541 taagcagaaa taaaaaggtt gggcgtaagc ttcaaggatc tagttcttca aggggtgaga
    24601 aggacatttt attgaagggg cctgcctata ctaagttatc tttagaggta catactttca
    24661 atgaaaaaag aaagcatgta cctctttttt aaatcgaaag gaagtgaagc aagtgttaga
    24721 agctttggaa caaatccgaa aagcagaaga agaaaatgaa aagcgtgctc aaacactgcg
    24781 gcagcagctg aagatctatg caacagaaaa agagcaggaa ttggaaacga tccaaacaaa
    24841 ctatcgacaa caactaaaac agttggtgga agaaaaagaa caatttgaaa tacaagaatt
    24901 agaaaaagaa aaaatgaaat tgaaagcaga agcagacaag gtgcaagcaa ctttgaaaga
    24961 tcaatatcaa aagcatcaga gtcaagcgat cggtgcgatc attgaaaggg tgaaagaaac
    25021 ttatggccgt cactaagatg gaaaaagtga ccctcatttc agataaaaga aatcaggaga
    25081 ggattttaca agcagtacaa ggaatccaaa acgttgaaat ccgggatctg tatcaaaata
    25141 gaacaaacaa ccagtgggtg gagaagtatt ttccagatac cacgattatt gataaagaag
    25201 caaaattaag cgctttaggt aatcggttga aagacatcca agaagctatc caattcatac
    25261 aacaccacgg cgatagccgt caaaagaaac tccatttgaa acgtcaagaa ctttccttac
    25321 aagatttgga agccaattat tccgaagaaa atttcataaa taaattgaaa gaagtccttg
    25381 atttaaaaga aaagtgggaa caactaactg aagaacgtga tcgtttagaa gaagaggaag
    25441 aatggcttct tcattggcaa cagctggata ttgctccaag tatgtatgca agtaagacga
    25501 ccatttttga gatggggact gtgagtgcag caaattttga aagtttcaag gaagaactga
    25561 gtaagcttga tgacgtttat atggaagaaa tagattatac gccaaaacaa gtttatcttt
    25621 cgtatgttgc tttaaaaaaa tcagttgctg ttatagaaga aattgctggg aaatacgggt
    25681 tcaataaaga aacgtatcct tatgaacaaa atcctaaaga aaaactgcaa gagaccaagc
    25741 agcaattatc cattgttcac gagcagcaaa agaagataac gacagcgtta ggtcagtgca
    25801 gtggctatat ccaagaattt gaatgggtag aagaagtcac acttgccgtt gcagaacgag
    25861 aaaaaataaa agaacggttt atccatgcga cttatctgat cgttttgcaa ggttgggtag
    25921 atgcagaaga aaaacagaat ttgctgcatg tgttaaacga aaccgtatca gaagaagata
    25981 tatacgtcag tttcgaaaca ccaaactcag aagagattca gcaggaagtc cctactaaat
    26041 tgaaaaacca tccgcttgtc gaaccttttg aattgctgac tgaaatgtac agtctgccaa
    26101 aatatgaaga agtagatcca acaccttgga tgacaccatt ttatcttgtc ttcttcggga
    26161 tgatggtcgc agatgttggt tatggtttgc tgatgctcat tgggacaata ctagctcaaa
    26221 aactacttgt tctaccacgc gggatgaaac gtttcgtaaa attctttgag attctctcga
    26281 taccatcgat catctgggga ttgatctaca gttcattttt cggacagtca ttaccaaaag
    26341 aactgtttgg catccgcttg cctttcccaa tcctatctac gacggatgac gtcaatacga
    26401 ttttgatttt atccgtgatt ttcggtctga tacagatttt ggttggatta tttgtggcag
    26461 caaaggaaaa tatcaggaga aaagattact tgagtgctgt cagtgatgga tttgcctggc
    26521 aagggatcct aattgggatc gttattgcgt tggttggtgc catgttgata aaaaatacag
    26581 catttgttta ccttggcgct gggctatcga tcattgctgc cttatgtatc ttgatcgttc
    26641 cgattatcca aacgccgtct aaagtcaaag gtgctgccaa aggtgcgtat aatctttacg
    26701 gactgaccgg ctatatcggt gatttagtca gttatacacg tttgatggca ttgggtattt
    26761 caggcggaag tattggagcg gcattcaata tgctagttgc tttcatgccg ccagctgctc
    26821 gattcagtgt agggatcctt ttgatcatcg ctttacacgc actgaatatg tttttgacac
    26881 tgctaagtgc ctatgtccat ggcgcacgat tgcagtatgt cgaatttttt gggaagtttt
    26941 atacaggagg cggacgtgcc tttgatccgc tgaaaacggc ggaaaaatac gtcaacatca
    27001 atcacaagaa aaaaaacaat aaataattgg aggattttta tataatgatg gattacttaa
    27061 ttagtcaaaa tggtggaatg gtatttgcgg tattagcgat ggcaacagcg acgatttttt
    27121 cagggatcgg ttcagctaaa ggtgttggaa tgacaggtga ggcagcagca gcattgacaa
    27181 caagtcaacc cgaaaaattc gggcaggctc tgattctgca attgcttcca ggcacgcaag
    27241 gtttgtatgg attcgttatt gcctttttga tttttatcaa cttaggaaat gacatgtcag
    27301 ttgtacaagg actgaacttt ctaggtgctt ctctgccgat tgcttttaca ggcttgtttt
    27361 caggtattgc ccaaggaaaa gtcgcagcgg caggtattca aattttagcg aaaaaacctg
    27421 aacatgctac aaaaggaatc atttttgctg cgatggttga aacatacgcg attttaggtt
    27481 tcgttatttc cttcctatta gtgttgaatg cctaagcata aaggaggaaa agcagatgaa
    27541 cgccattgaa aaaatcatct ctcagatgaa cgaagcagca gagcaggaaa gagcggcgtt
    27601 agaacaagaa gagcgaatga agatcgatca aaactttgaa caaaaacgta cgcaagttga
    27661 aacagaacat caaaaacaaa aagaaaaaca aatcgaatta ctcgaaaaaa aataccgtca
    27721 gctaaggaat cgccagcaag tagaagtacg acaagaaaat ttgaatgcga agcaggaatt
    27781 tttaagacga ttgtttgcag atgcggtaac tgaaatggaa aattgggatg aatctgaaca
    27841 aatccagttt atcaaaaatg cattgtattc attacctttg acaggaaaag tagcattcat
    27901 tgctggagaa aaatcagcag cctatctttc tcagacgttg ttggatgaat ggaacaatga
    27961 acttcccttt atgatggttt taagtgatga aactgttgct gatcaagcgg gttttctgat
    28021 aaacgatcaa ggagttcagt ataattttct tttcagcagc cttgtacaag atatccaagg
    28081 aacgatgagt tttgagattg ccaatcaatt attcgaataa ggagggatca gtgtatggaa
    28141 taccacgaac taaatccctt gattcgcgga agagagcttg aacttttgtc caatgagaca
    28201 tttgaacgga tgattcaaac aaattctgtc gaagcacttg gtgaaatatt gaaatccacg
    28261 atttacagtc catatattta tgacggatat gaatatgact tcgaacagaa tcttttaaag
    28321 gagcagagcc gtctatttcg ctggttaaag gaatcagcac cggagcctga aatcgtttgg
    28381 atctatacga tgcgttatac tttccataat ctgaaggttt tgacaaaagc tgagatgaca
    28441 aatcaaaatc tggaccattt gtatatcgac gatggatttt attcattaga aaccttgaag
    28501 gatgccattc atacgcaagt ttcaaatgaa cttcctgaaa gcattatgga cagtatacga
    28561 gaagtacgtg attattttga agagtctacc attcttcagg gaattgacgt gatttatgat
    28621 cgacagtttt tgacagagca gcgacggctt gccgataagc taggatatca agaattgctg
    28681 acagaagtca ttgcttttat tgacttgacc aatatcacaa cgatggctcg aggaatattg
    28741 cagaaaagaa catatgggtt catgacgacg gtactttcca gctcgggaag tatcgaaaaa
    28801 gaagtatttt tgaactttgt agataaagat atggaagatt acacgaattt tctattagag
    28861 acagattatg ctgattttct gcgccctgtc atccgtgagg gaaatattga tctcgtaaag
    28921 cttgaacagt tgaaagatga ttatctaagt tcattgtatc aagaagcaca aacccaggct
    28981 ttcggaccac tgccactact agcctttttg aacgcaaaag aggtagaaag caaaaacctt
    29041 cgtttactgg ttatcggcaa aagaagccat ttcgaatcag aaacaatcag aaagcgggtg
    29101 agacaagtct atgacgcata agatcggtgt agttggcgac aaagattctg ttttgccatt
    29161 caaattgttt ggctttgatg ttcgttatgg tacaaccaag caaaccgttc gtcaggcaat
    29221 tgaagaaatg gcaaagaatg agtatggcgt gatttatgtc acagagcaat gtgcagcatt
    29281 gagcccagaa acgatcgaac gttacaagga acaaatgata cctgctatcg tattgattcc
    29341 caatcatcaa ggatcattag ggatagggct ggaagaaatt caaaaaaacg tagaaaaagc
    29401 agtaggtcaa aatatcttat agtatgaaaa ggagatagaa attttgcaaa ttggaaaaat
    29461 cataaaagtc tccggacctc ttgtaatggc tgagaacatg tctgatgcca gtatccaaga
    29521 catgtgtcta gtgggagatc taggagttat cggagaaatt atcgagatgc gtggcgatgt
    29581 ggcgtctatt caagtatatg aagagacttc tggaattggt ccaggcgaac cggttcgctc
    29641 tactggtgaa gctttatctg ttgaattagg accagggatc atttcacaaa tgtttgacgg
    29701 aatccaacgg ccattagata cctttatgga aatcacacaa agtaatttct tagggcgcgg
    29761 agttcaattg cccgcattgg accatgagaa aaaatggtgg ttcgaaccaa cagtagaagc
    29821 cggagaaacg gtgagtgctg gagatgttat cgggatcgta gaagaaacaa aagttataaa
    29881 acataaaatc atggtgccaa atgggataaa aggaacgatc aaaacaatcg aaaaaggttc
    29941 attcactatt gatgaaacag tatgtgtcat tgaaaccgaa aatggtgaca aaaatttgac
    30001 gatgcttcaa aaatggccag taagacgtag tcggccaatc aaagaaaaat taaacccgga
    30061 tgcacccatg atcactggac agcgagtgat tgatacattc ttcccagtga caaaaggagg
    30121 agcagcagct gtacctggtc cttttggtgc agggaaaaca gtcgttcagc atcaaattgc
    30181 aaaatgggcc gatgtggaca tggttgtcta tgtgggatgt ggcgagcgtg gaaatgagat
    30241 gacggatgtt ttaaatgaat tccctgaatt gattgaccct aatacaggag aatcgttgat
    30301 ggaacgtacg gtattgattg ctaatacatc aaacatgcca gtagcagctc gagaagcttc
    30361 tatctatacg ggaatcacaa ttgcggaata tttccgtgac atgggttatg acgttgctat
    30421 catggcagac tccacttcac gatgggcaga agcacttcgt gagatgagcg gacgtttgga
    30481 agaaatgcct ggtgatgaag gttatccagc ttatcttggt tcacggcttg cggaatacta
    30541 tgaacgatct ggaagagtca ttgcattagg atcagaaggt cgcgaaggaa gtataaccgc
    30601 aatcagtgct gtatccccgt ctggagggga tatttcggaa ccagtcacac aaaatacatt
    30661 gcgggtcgta aaagtattct ggggtcttga ttccagttta gcccaaaaaa gacattttcc
    30721 ttcgatcgat tggatacaaa gctattctct ttattcaaca gaagtaggga agtatatgga
    30781 tcatatcctt caagctgact gggcatccat ggtcaccgag ggcatgcgta ttttgcagga
    30841 agaacagcag ctgaatgaaa tcgtgcgttt ggtagggatt gattcccttt cagataatga
    30901 ccgtctgaca ttagaagtgg caaaatccat tcgtgaagat tatttgcagc aaaatgcttt
    30961 tgatgatgtc gatacattca cttcaagaga gaaacaattc aaaatgttga agatgatctt
    31021 gacctttggt gaagaaggac gccgtgcgtt gtcgttaggt gcttatttaa gtgagatcat
    31081 ggacggtacc gtatcgattc gtgaaaggat cggacgaagc aaatatatcc cggaaacaga
    31141 attagaaaaa ctagatcaaa ttattggtga aatcaagcaa acgatgcaaa cgatcgtttc
    31201 agaaggaggc atgagcaatg attaaagaat atcgtacgat cggtgaagtc gtcggtccat
    31261 tgatggcggt tgaaaaagtt gctggtgtca aatatgaaga attaatcgaa gtccgtatgc
    31321 aaaatggcga gattcgtcgt ggacaagtat tggaagtcca agaagataaa gcaatggtcc
    31381 aaatattcga aggaacaagt ggtatcaatt tacgtgactc ttctgtacga tttttaggcc
    31441 atcctttaga actaggtgta tcggaagaca tgatcggtcg agtattcgat ggtttgggac
    31501 gaccgaaaga taacggacca gagatcctgc ctgaaaagca tttggatatt aacggagaag
    31561 taatcaatcc agtagctcgg gattatccag atgagtttat ccaaacaggt atctctgcaa
    31621 tcgatcattt gaatacatta gttcgtgggc aaaaattacc agtattttct ggctctggtc
    31681 ttcctcataa agaattagct gctcagatcg cgcgacaagc aacggtactc gattcatcag
    31741 atgatttcgc cgtagtcttt gctgctattg gaatcacttt tgaagaagct gaatttttca
    31801 tggaagattt tcgtcaaacg ggcgcaatcg accgttctgt catgtttatg aatttagcaa
    31861 acgaccctgc aatcgaacgt atcgctactc ctcgtatggc tttgactgct gcagaatatc
    31921 ttgcttatga aaagggaatg catgttttgg ttatcatgac agacatgacc aactatgcag
    31981 aggcattgcg agagatttct gcagcacggc gagaagttcc aggaagaaga ggatatcctg
    32041 ggtatcttta cacgaactta gctactttat tcgaacgagc aggaagaatc cgcggactaa
    32101 aaggatcggt tactcaaatt ccgattctaa caatgccaga agacgataaa actcatccaa
    32161 tccctgactt gactggttat attacagaag gacagatcat cttgactaga gagctttaca
    32221 agagtgggat tcagccacca attgatgtac tcccgtctct ttcgcggtta aaagataagg
    32281 gaactggacc cggaaagaca agagaagacc atgcagctac gatgaatcag ctttttgcag
    32341 cctatgcaca aggaaaacag gcaaaagaat tagccgtagt tttaggtgaa tctgctttat
    32401 ctgatgtaga caagatctat gcgaagtttg ctgagcggtt tgaaaaagaa tatgtcaatc
    32461 aaggattcta cacgaaccgt tcgattactg aaacacttga tctagggtgg gaactgctat
    32521 cgatgttgcc aagaaccgaa ttaaaacgaa tcaaagacga catgctggac aaatatctgc
    32581 cagaagggaa gtgagttctg atggcacgat tgaatgtgaa tccgacccgc atggaattga
    32641 ctcgattgaa aaaacagttg acaactgcca caagagggca taaattatta aaagataagc
    32701 aagatgaact gatgcgtcaa ttcatcctgt tgattcgaaa gaacaaccag ctgcgtcaag
    32761 aaatggaaga gtctatgaca gaagcaatgt ctgattttgt attggcaaat gcttcgatca
    32821 cggaaccttt catcgaagaa ttatttgttt tgccggcgga aaacgttgat ttgtctatcg
    32881 tagacaaaaa tatcatgagt gtaaaagtac ctgtgatgaa ttttgaatat gatgaacagc
    32941 tgacagacac acctttggaa tacgggtatt tgaattccag tgccgaactg gatcggtcaa
    33001 tcgatcggtt tactaccctg cttccaaaac ttttggaact gacagaaatc gaaaaaactt
    33061 gtcaactgat ggcgagtgaa atcgaaaaga cacgtcggcg agtcaatgca ttagaatata
    33121 tgacgatccc tcaattagaa gaaacgatct attatattcg tatgaagctg gaagaaaacg
    33181 agcgcgcaga agtaacaagg ttgatcaaag taaaaaatat gggtaccact gactaagtgg
    33241 tatctttttt taaaagagga aaacgatgat taaacaaagc aaacaaataa agaaacgttt
    33301 gccggctgtt cagcttctcg cattaggatt ttttctcctg attttagttg gcggcggcct
    33361 acttacccta ccatttttta gtaatagtgg gcatgggaca aattttatcg atgcgctttt
    33421 cactgcaact tctgctgtct gtgtaacggg attgacaaca ttgaatacag cagagcattg
    33481 gaacagtgct ggtcaatttt tgatcatgct tttgattgag atcggcggtc ttggttttat
    33541 gatggttccg attctatgct ttgcattgat aaagaagaaa gtcagtctca gcacacgtat
    33601 tatattaaaa gaagcgctga atctagaaga agtatcaggt gtgatgaagc tcatgcttta
    33661 catcctaaaa ttggcctttg tgatccaatt gattggtacg gttgctttat cttttgtatt
    33721 tgtgcctgaa tttggctggt tgaaaggtat ctggtacagt ttgttccatg caatctctag
    33781 tttttgtaat gcagggtttg atttgttagg agacagttta gccaatcacc agcaaaatgt
    33841 ttatttgatc atggtggtat ctgctttgat catttctggt gggttaggct tcatcgtatg
    33901 gcgtgattta ctggaatatc atcagaaaaa gaagatgacg attcattcaa aaatcgcatt
    33961 aaccgttaca ggtacccttt tattaggcgg attcgttgtg ttctttttca cagagcgcaa
    34021 tggggcaacg ctagtggaag ggaatatgtt tcaacgattg gcaaacacct tttttatgag
    34081 tgtgacacct agaacggctg gatattattc agttgattac ctccatatga gccatgcagg
    34141 tctgatttta acgatgtttt tgatgtacat cggcggaaca tctgggtcga cggccggcgg
    34201 attgaaaaca actacgttag gtgtgttgct gatccagatg cgttcgatgt tgaaaggacg
    34261 tacgagagcg gaagcatttg gccgtaccat ccgacaaggc gcagtgttgc gggcacttac
    34321 tttatttttt gttacgttga cgttgtgtgt agtcgccatc atgattttat cggtgactga
    34381 aacgatacct aaaacttctg gcatcgaata tattgctttt gaggtcttct ctgcgtttgg
    34441 tacagtaggt cttacgatgg gattgacgcc agacttgact gcaataggga aattgatcat
    34501 tatagcgctt atgtacattg gacgagttgg tgtattgact gtggtcttct ctctgatgac
    34561 aaaagcacaa agaacacaat caaaatacaa atatccagaa gaaagtgtca tgattggata
    34621 atttcatctt ctgacagaaa cctagtgatt tagtaaaata agaaaataaa ataatttttc
    34681 ttgcttcttg gatactttaa gtgcaaaatg ttcgtttatt ttttgaggca ctacccgttg
    34741 ttgaacctcg ggtagtgcct cttttttatt gaaaggaatc aataagattt gattccttta
    34801 tttatcaata ggttataatt catatttttt tattttttct tttacgatac ttagtccaga
    34861 agctaaaatt tcaaggttat tagaggatag ggaaattcga acgaatgcat ctggttgcgc
    34921 acgtgtagta aagcgtctgg aatgataaag tcggataccg ttagcaagaa agtcattctc
    34981 aattatctta gggtcaatct caggatcaac tggaagaatt cgatagtaag ggacacgatt
    35041 cgttggtttt tctactttga aaagagaatc aaaaagctga ttggatcttt ttgtaagact
    35101 taatttagtt tctaagatcc tatttgcttc atttgaatgg aggatttgag tgatgacttc
    35161 cacatctaat ccagaagttt tcacatttat attaaaaatt gcttgttcta tcttgctttg
    35221 gacatgtact ggaactacaa gataagcaac tcgcaatcca gaacaaataa attttgtcat
    35281 tccagctaag tagattgttt ggttaggtaa taaattttgg aaaggaggaa tgatcgtatg
    35341 ttgatagtaa gttgttaaaa atgaatggat atcatcttca ataacccata gaccttcttt
    35401 ttcaatcact tcggctaatt gctctcgtct tttgtgagag atttgaaacc ctataggatt
    35461 attacaagaa ggcataagaa agacaccatg aatcttcttt tttctacatt cctgaatcaa
    35521 tatatcaggt cgcattcctt gctcgtcaaa atcaattgga acgatctcta aatgatataa
    35581 ctgagccaat tcaatgaaat tagcgtatgt atatcggtct gttgctattc tatcacctgg
    35641 agagaaaagt gcagaaagtg tgatagccaa tccattttga acaccggaaa caatagatag
    35701 agattctaag gacggaatga ctccttgagt ttttaaccaa tttgctgcag tagtcaattg
    35761 aaaatttgtg cctttgggat cttggtaatt aagcaaacca acaagatttg ggttattact
    35821 agttgattga ataaacggga caacgaattt attacattct tcaaaagaac taaccagacc
    35881 aaaatcaatg atatgttcgg ttacttcatt ggtagagatg gtgatggact caaagacatt
    35941 gggagaaata aatgtgccac ttccaatatt cgtatacagc aaacctttct caattccata
    36001 tttataagcc ttgccaacgg tagtaaaatt taaatctaaa taatcagcta gctctctttg
    36061 ggagggtaac tgagtgtttt tctgtaattt attctttaaa atatcttgtt ctaattgttg
    36121 aatcaacgat cgataataag gacgcttcaa tttttgtttg tctggtcgcc aagttaacgg
    36181 gtaattgtca taagaattaa taggcatttt ctccactcct taaaattgta taccatacaa
    36241 ttttaccatt gattgtattg attttagaag ttaatctttt gtgaggaggg aattgtctat
    36301 gaaaattatt aattttgagg aagtagtagc ttcttttagt tttgaagaag cgattcaagt
    36361 aatgaaaaaa tgtttttttg attatgaaag tggtaaaatc agccaaaatc ctcgattggt
    36421 ggaaatttta cctgacggaa aaggcaaaaa tatgtttgcg atgatgcctg cttatttggg
    36481 ggagaacaga tattttggca caaaagtcat taccgctttt ccggataatc atcagtatca
    36541 tttaccttct catttaggag agattttctt gtttgatgct caaaacggac tacccaaagc
    36601 attgataaac gcaaatgcag tcacctggat tcgaacagct gctgtatctg cgctggcaac
    36661 agattactta gcaaatccgc atgcagaaac acttgcttta attggagctg gtcaacaagc
    36721 attgtcgcat ttgactgcga tttctaccat tcgtcccatc actacagtgt ttgtttacga
    36781 tattttagaa gaaagacgaa atacttttat cgctaaagct aaaaaacagt atcctaaaat
    36841 aacgtttata aatgcaatat cagtagaaga tgcagtacgt ccagcggaaa tcatttgtac
    36901 gttgacctct agtaaagagg catttcttca aaaagagtgg gtaagggaag acgcacatat
    36961 caatgctgtg ggcacattta ctcctgatac aagagagata agtacatcgt tgatggaaac
    37021 ttctcaaatc tatgtagatg attactcagc tgccttaaca gaaagcgggg acctgcttat
    37081 agccatggca gaaggagatt tagaggaatc ttgtattgtt ggtacattag gcgaattagt
    37141 ttctgagaaa aaaacatttt ataaaggaga atcgaggccg acgattttcg acgcagttgg
    37201 tctagctatt gaagatcttt gttgtgcaga atatatttat aacaaattag agaaaggggc
    37261 tttaaaatga acttagatta ttacgtagtt gatgcatttg cagatgaggt gttcaaagga
    37321 aatccagcag cggtttatgt gctagaagaa tggctgccag aaggtacgat gcaaaaaatt
    37381 gccattgaaa acaatttatc tgaaacagca tttacagtga agaaaaacca agaatttgag
    37441 cttagatggt ttacgccaga tcgagaaatc gatctttgtg gtcacgcgac attagcaaca
    37501 gcatttgttt tatttaacta ttacaaaata cctgatgaaa cgattaaatt ttctactcaa
    37561 agcggtaatc tatttgtgac aagaattaaa gattattatt acatggattt tcctagtatt
    37621 atgccgaaaa aagtgccgat attgtctgaa tatgaagaag cgattggggc taagattaaa
    37681 gaagcctact tagcacgtga tttatttttc gtattagaag atgaaaaaac ggtggctaaa
    37741 ttgcagccag attttacagc tatcaaaaat tttgaattag gaataggagt catcgtgaca
    37801 gcagaaagtg tgcaaaaaga ctttgtttct agaaccttct ttccaaaatt aacaatcaat
    37861 gaagatccag tatgtggatc agcacattct aatcttattc cttattgggc agaaagaatg
    37921 aataaaacaa aattgtcagc ttatcaaatg tcatcaaggg gcggggatct agaatgtgaa
    37981 ttaaaagaag atcgtgtgat aataggagga aaagcagttt tgttttctaa aggtacctcg
    38041 tttatttgat ttctttcaaa tgaattaaaa ataaattggt ggagagtggc aaccttatag
    38101 ctacaatggt tgttaaaaaa agacgcaaac tttttcaatt taatgaaaag tttgcgtctc
    38161 attttaatta ttggtgtttg ttttttattc aatcaagagt aaaatgcccc atactaacta
    38221 tcctcggatg acttcgattt tataaccatc agggtcaatg acaaagtagt aagaaggcgc
    38281 agtgccaggt aatcctttta agtcagtaat ctcaagacca gcagctttat gttgctcgtg
    38341 taatttttct agatcatcag ctgctatagc gatatgacca tagccgtctc ctagatcata
    38401 cgcaccatga tcatagttgt aagtcaactc taattcatag tcatccccag gaagtgtcaa
    38461 ataaacaagt gtaaatttgt tttctggaaa atcacggcgg cggctttctt caaaaccaaa
    38521 tgccttttga taaaattcga ttgatgcttc aagatctttc actcgaacac atgtgtgagc
    38581 cattttcata gattcatctc tccttttcct tttactcaat cataacaaaa agaagtaagg
    38641 aaatagttga tttgtgcttg ttcatgagaa atttctcatg taaaatggat agaaaataaa
    38701 actgttaagg tggaaactat tcatggataa accaattttt ggtgaaaaaa agcaatttgc
    38761 tgattatcaa acccgttatg cagcatatat tgtcatagaa agagatcagc agattgcagt
    38821 aatcgaggct ccaaatggtg ctttttttct tcctggcggg gaaattgaag gagaagagac
    38881 gaaagaagaa gcgattgtcc gtgagctgat agaagaaatg ggaattgtgg cagaaatttc
    38941 ttcttatctg ggagaagctg atgagtattt ttactcgaat tatcgtgcaa cttattatta
    39001 taatccagga tatttctttg ttgctgattc ttggaagcgt ataggagaac caactgaaaa
    39061 gaccaataaa atttggtggg tgagccctca agaagcgctt gtgaaattga aacgtggtag
    39121 ccaccgatgg gcagtcgaga gatggatcga aaaaaataaa aactgaaaga aagcgagtag
    39181 atcagctttc tctcagtttt tttatttatt acagtacata ttttttgatt gcttcagcta
    39241 caccgtgctc gtcgtttgat gaagtgatga catttgctgc ttctttgaca agagaaacgg
    39301 catttcccat tgctactcct agaccagcat attcaatcat ggataagtcg ttctcgttat
    39361 ctccgatagc catgatttca tcttgatcaa taccaaggtg ctgtgcgaga ttcgcaactg
    39421 ccgcaccttt acttgcttct ttatttagta cttcgaagta atagggcgca cttttgaccg
    39481 ttgtgtattt atcacggaaa gtttgcggta aacgcgcaat tgctgcatct aaaatttctg
    39541 gttcgtcgat catcatcatt ttgacgatgt tcatgtctgg tgtcatttct tcaggtgttc
    39601 gatattttag cggcatcttg actaaacttg cttcgtggat cgtataagcg ctgatatctc
    39661 gattaggcgt gtagatcgct ttgtcatcga tcgaatggag atgactgccg acacggcgtg
    39721 ccattacttc gatctctagg aaatcttcat gtgtcagtcc gtgacgggcg atgattttac
    39781 ctgatttcgt ttgttgtacg agtgccccgt tataagtaat aacgtaatca ttatcttgaa
    39841 aaaggttcaa ctcgtttaac tgttcctgga cacctggtaa gggacggcca gtgcataaaa
    39901 cgatcgaaac accttttgca acagcagcgg caatcgcttc ttttacttct tttgttactt
    39961 ctcttttctc atttaataaa gtaccatcga tgtcgatagc gactagtttg atagacataa
    40021 tttcctccaa tatattttaa tttatttcaa tcaatgcgcc attacggata tggctggcaa
    40081 attcttgata agtttcgtca aataaatcat aatgatcacg caaggacgga tcaaccattt
    40141 cttttggaaa gaaaaatcgc tcatctcctt gttcttgtcc cgctaatgca gcaacaagct
    40201 gactaacctg tgataattct atcaaagttc cgtcatttcg caaaatctcg atttgtgtgc
    40261 gatgtctgcc ttgttctggg cgataaaaat cataaggtag atcgtagcta gaattcacag
    40321 cagtgtaata aacaggatta tatccgacct tttctactaa caaagtgagt tcttggacta
    40381 attttgaatc ccggttgcca gaaaaagtag cagatttcag tggtttacga ttcaaaaaac
    40441 gtttagctaa atcatttaaa ataggatcag cagattcaga ccattgagta aagtaagtgc
    40501 ccaagacccc atcatcaagt ttgaggtatt cttctaaagt aaagcttccg tcaagaaatg
    40561 gcagaagtaa ttgagaatgg agttcaaaac tgctggcatc ttcttgatac aactcgtgtg
    40621 cgcgatgaag cagatgctcc aaaattactt ccataccacg ggaaacagga tggaagtaca
    40681 cttgcacata catttgatat cggctaacga tataatcttc cactgcatgc atgccactca
    40741 tggaaaaagc gatcccgcct tcataaggac gaatcacacg taatattcgg gtaagatcaa
    40801 atgttccata ttctgttcca gtgaaataag catcccgaag caaatagtcc attcgatctg
    40861 catcgatctg actagaaatc atttggacaa cttgtgggtt ggggtaagtt ttttggatca
    40921 cactagctac cttttcagga aaaccttctt ctactcggtt aagaatctga tacacttctg
    40981 tttctggaga agtgatgatc tctacagtga tggcttcatg attcgtatgg aagatatgtt
    41041 caaatgtatg agagtaagga ccatgaccga catcatgcaa cagtgccgca cataaagtca
    41101 ccagccgttc ctgatcatcc cagcctccgt tgcctatttt ttcttttgaa aaatttcgag
    41161 aaaagatatc gcagattcgt cttgaaatct cataaacgcc taatgaatga gtaaaacggc
    41221 tatgttctgc tccgtgaaaa gtgaaagaag aagtgcctaa ttgtttaatg cgtcgtagac
    41281 gttggacttc tttcgaattg atcaaatcta agattacttg atgctggaca tggatatagt
    41341 tatggactgg atcacgaaaa actttttcca taggcagcat ttgatattta taaggggttg
    41401 tcataatgta gctcctttat gttattacgt tgttccattc tctggatttg gttgttccat
    41461 tgctccagct ggatttcttt ttttagccag tcggtagaat cctgtaaatc acttgcagaa
    41521 tacagctttt caaaagcttt tgcaaatgct tctttgactt cttttacaaa aaggggttgg
    41581 tcaagcagtt cttcaagtgt tgccattgta gcaggatcca ctgcaggata gccatttgta
    41641 ccaaagtctt cttttaaagc agattgataa aatcgcttca tgacttgccc acgtttttcc
    41701 tgattaccgt tgacacttaa gtacatcata acagaaatcc catttttgat ccgacgctga
    41761 gcgataccgg caaatttttt gccatggatg cttaaatcat aagttccagg acaataagaa
    41821 tgggtgattt cgaatgcttg tatcggtgcc tttggaaaag cgtatttagt caataggagc
    41881 atttgctcgt aagcgtcatc tatggttatt ttttttgtgt cactttgggg gaaaatcaaa
    41941 gaaatattca aaattccttc atctgatacg atccctaatc cacctgaatt tctgatcact
    42001 ggagcgtaac ctgcatcttc cagtgtttgg atcccgtcac ttagaaaagg agttcgaaca
    42061 tccttcatac ctaagatgac tgtgcagtca tattgccaga aatgtaaaaa gatttgctgg
    42121 tgttctttag aatattccgt taatatatct gttaatgcaa aaggggaaaa gtcgtttttt
    42181 tgataaatgt gctgatctag tcgaatcgca gggttcattc aaccggctcc tttgtataaa
    42241 ttcatatttt ctacaatatt atactctaaa atcaaagagt tgttttggaa aaaaggtttt
    42301 ctgtaaaaat ggacaagttg gttgacaaaa caaaagaaac taggaacaat gtagctatga
    42361 gaagtaaagg agaatgaata atggaattat cacaaggaac accgatttca atcaaattgc
    42421 gtacaaaagt aaaacagcaa ggggaagtgc aagattttta ttttgatttg aaagggcaaa
    42481 tggtgacaat tggcgatacg ctgtacatac gttataaaga gattcaggag gagacgggcg
    42541 atgagattcc tgtaacgatc aaattagttc cagatggtca tgtacagctg atccgtgcag
    42601 gagaaatgcg gatgcgtttg aaatttggct ataaagaacg gttagaaaca tcctatcgaa
    42661 caccatacgg tatgatccag attgctactt ttacgaaaga actgcatgtt agtttgaaag
    42721 accgtcccac agcagggaag gttcgaatcg attatgatct gttcatggga ccggaaaaaa
    42781 tcggcgaata ttatttgacc ttggatttta cagcgtaaat ataaaagaaa agtttgattt
    42841 tttgtgtctg attttgtatg ataaggagta gactgtgaaa ggacgtgtcc tgattggaaa
    42901 ttagtgtatt tgaaggtgca aacaaaagcg aattatcaat gatcgaagta gcacacgcga
    42961 tcttagaaca acgtggcgat gtcatggatt tttctgattt agctaaccag atccaaaact
    43021 atcttgggaa atcagacagt gaaattcgtg attcactcgc acaattttat actgacttga
    43081 atattgacgg cagctttatc tcattagggg ataatcgttg gggcttgcgt tcatggtatg
    43141 ctatcgattc gatcgatgaa gaagtaaacc atggaatgga cgaagaggat gaagatactc
    43201 cacgtcgccg taaacgcaag aaagtcaacg cctttatcaa tgatgatgaa gacgcgatcg
    43261 attacaatga cgatgaccca gaagatacag atctgacaga agaagatgac gatgatctgt
    43321 ttgatgacga tgatgatgaa gatgaagaaa tcgctgctta taattctgac ttgcaagaaa
    43381 tcggagcaga tgacccgaca gatgatgaag aagacttgcc aggaaatatc gaagaagatt
    43441 taagtatcat agacgaagac gatgatgatg aagattatga agaggaagaa ttttctgact
    43501 ctgaaaaaga ataaacatta ttttcttacg agaggttgtg acagagacga tcagcctatc
    43561 agaataagga ggtgtgacac gattttt
//
LOCUS       DACDYI010000016        43441 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.016, whole genome shotgun sequence.
ACCESSION   DACDYI010000016 DACDYI010000000
VERSION     DACDYI010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.016"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(105..629)
                     /locus_tag="IWM52_000843"
     CDS             complement(105..629)
                     /locus_tag="IWM52_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9973115.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="IWM52_000844"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="IWM52_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAP9973116.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="IWM52_000845"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="IWM52_000845"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP9973117.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="IWM52_000846"
     CDS             complement(2351..3031)
                     /locus_tag="IWM52_000846"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP9973118.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="IWM52_000847"
     CDS             3157..4026
                     /locus_tag="IWM52_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9973119.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="IWM52_000848"
     CDS             4052..4567
                     /locus_tag="IWM52_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973120.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5177)
                     /locus_tag="IWM52_000849"
     CDS             complement(4614..5177)
                     /locus_tag="IWM52_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9973121.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="IWM52_000850"
     CDS             complement(5232..5411)
                     /locus_tag="IWM52_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973122.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="IWM52_000851"
     CDS             complement(5488..5997)
                     /locus_tag="IWM52_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973123.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="IWM52_000852"
     CDS             complement(6093..6473)
                     /locus_tag="IWM52_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP9973124.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /locus_tag="IWM52_000853"
     CDS             6803..7552
                     /locus_tag="IWM52_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAP9973125.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7736..9013)
                     /locus_tag="IWM52_000854"
     CDS             complement(7736..9013)
                     /locus_tag="IWM52_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973126.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="IWM52_000855"
     CDS             complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="IWM52_000855"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAP9973127.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9935..10879)
                     /locus_tag="IWM52_000856"
     CDS             complement(9935..10879)
                     /locus_tag="IWM52_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAP9973128.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10881..11567)
                     /locus_tag="IWM52_000857"
     CDS             complement(10881..11567)
                     /locus_tag="IWM52_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAP9973129.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            12041..12361
                     /locus_tag="IWM52_000858"
     CDS             12041..12361
                     /locus_tag="IWM52_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP9973130.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            12515..13420
                     /locus_tag="IWM52_000859"
     CDS             12515..13420
                     /locus_tag="IWM52_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9973131.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            13417..14190
                     /locus_tag="IWM52_000860"
     CDS             13417..14190
                     /locus_tag="IWM52_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9973132.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(14371..15795)
                     /locus_tag="IWM52_000861"
     CDS             complement(14371..15795)
                     /locus_tag="IWM52_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9973133.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            16006..16452
                     /gene="nrdI"
                     /locus_tag="IWM52_000862"
     CDS             16006..16452
                     /gene="nrdI"
                     /locus_tag="IWM52_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP9973134.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(16517..18499)
                     /locus_tag="IWM52_000863"
     CDS             complement(16517..18499)
                     /locus_tag="IWM52_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9973135.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(18513..19409)
                     /gene="murQ"
                     /locus_tag="IWM52_000864"
     CDS             complement(18513..19409)
                     /gene="murQ"
                     /locus_tag="IWM52_000864"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP9973136.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(19412..20473)
                     /locus_tag="IWM52_000865"
     CDS             complement(19412..20473)
                     /locus_tag="IWM52_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP9973137.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(20684..21211)
                     /locus_tag="IWM52_000866"
     CDS             complement(20684..21211)
                     /locus_tag="IWM52_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9973138.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(21278..22780)
                     /locus_tag="IWM52_000867"
     CDS             complement(21278..22780)
                     /locus_tag="IWM52_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP9973139.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(23038..24159)
                     /locus_tag="IWM52_000868"
     CDS             complement(23038..24159)
                     /locus_tag="IWM52_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9973140.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            24285..25226
                     /locus_tag="IWM52_000869"
     CDS             24285..25226
                     /locus_tag="IWM52_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP9973141.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(25376..26272)
                     /locus_tag="IWM52_000870"
     CDS             complement(25376..26272)
                     /locus_tag="IWM52_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP9973142.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            26541..27881
                     /locus_tag="IWM52_000871"
     CDS             26541..27881
                     /locus_tag="IWM52_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9973143.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            28099..28542
                     /locus_tag="IWM52_000872"
     CDS             28099..28542
                     /locus_tag="IWM52_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP9973144.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(29046..30464)
                     /gene="nhaC"
                     /locus_tag="IWM52_000873"
     CDS             complement(29046..30464)
                     /gene="nhaC"
                     /locus_tag="IWM52_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP9973145.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(30867..32090)
                     /locus_tag="IWM52_000874"
     CDS             complement(30867..32090)
                     /locus_tag="IWM52_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAP9973146.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(32121..32960)
                     /locus_tag="IWM52_000875"
     CDS             complement(32121..32960)
                     /locus_tag="IWM52_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP9973147.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(32977..33657)
                     /locus_tag="IWM52_000876"
     CDS             complement(32977..33657)
                     /locus_tag="IWM52_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9973148.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(33659..34732)
                     /locus_tag="IWM52_000877"
     CDS             complement(33659..34732)
                     /locus_tag="IWM52_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9973149.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(35379..36050)
                     /locus_tag="IWM52_000878"
     CDS             complement(35379..36050)
                     /locus_tag="IWM52_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9973150.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(36137..38815)
                     /gene="mgtA"
                     /locus_tag="IWM52_000879"
     CDS             complement(36137..38815)
                     /gene="mgtA"
                     /locus_tag="IWM52_000879"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAP9973151.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLAFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTRFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            39474..39911
                     /gene="ndk"
                     /locus_tag="IWM52_000880"
     CDS             39474..39911
                     /gene="ndk"
                     /locus_tag="IWM52_000880"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAP9973152.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(39933..40799)
                     /locus_tag="IWM52_000881"
     CDS             complement(39933..40799)
                     /locus_tag="IWM52_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9973153.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(40959..43052)
                     /locus_tag="IWM52_000882"
     CDS             complement(40959..43052)
                     /locus_tag="IWM52_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP9973154.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT        12542 a         9100 c         7667 g        14132 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct attttttata
     7741 gtaattcctt cagatacttt cttgcctcat cattgaaacg cagtgattct tctaacttct
     7801 gataatattc caacgcttgt ttctgagctt cagtaaaacg ataatgtttt cctttccatt
     7861 tgtcgattgt ctcttgcaca tttccagatt ggatatcttt cactactagt tgccattttt
     7921 cttttgagac tgtacggtct ttcttatcag cagattgtat agcttctcgt tctttacgca
     7981 atacttttaa aaaaccattt ctgactatcc gaggaatgct gtcgaagaac ccttctgtaa
     8041 aaccatagcg acaatattgc ttccaaataa atagatagat ttttggttgg atgaatcgtt
     8101 ggtttttcgc aaaatattcc atctgagcag tgacttctgg tgttcgttga cgttttgtct
     8161 ttctcaacac ggaaataaat gtttcaaaat aagcattagg atgtactggc acttcttcat
     8221 atacgatgga ttgccaatct ttacgtaaaa atgcgcgata gttgatcaag aattcttcta
     8281 catcctttac taattcttct gcaaagtcgt caaatagttc tttttttatc tgtcgctttt
     8341 ttgactgccc attgatgtgt tcacttattt tcttgatttc attttccaat gcttgataat
     8401 ccgctatgta aatcggcatt tctacttggc gaaattctgc gagccgctga gctaatttta
     8461 tatccggtaa atatggctgt ttcatccgac gattttggta caagacaaaa cagtattttt
     8521 gccatagtcc ttcaggaaaa tcgatattct tctgcttaag ccataataat tcgtctaagg
     8581 ccagatcaac ttttgtcatt ccctgatgga tcgaagctgc cactgtcgga gagacatgca
     8641 ggtgatcggc aaagtggttg atgcctagtt ttaggatctt tccggttttt ggcgatttaa
     8701 cgatgtattg cacttttaat tctctaccac actcacaata caatttttgc tggcttcgtt
     8761 ttgttcgaaa aaacggattg atcttgaagt cagaaaacac ccaatctgtt gcagctaaat
     8821 aatgttcttc cataaaacga gagctgatta gatatcggcg gtgcttatcg actagttccc
     8881 gctgtttttt cgttaatttt gcatagatct cccaacgttg actgcttgtg tattgtttag
     8941 aaattttgat gggatagctt ttgctgattt cttttggaaa ccattcttca aagacggatt
     9001 gccattcttc cacggctgtt tctttccttt ctaattacaa aaagctggct actaacataa
     9061 cgttagcaac cagccagtgt ttcttgctaa attagtgagt tgttccggca tttccggaaa
     9121 tatcttcttt attttctact gctgcttcaa ctgcataagt caaagaagaa gcaatgtctt
     9181 ctaaagacat agaaggcgta cccactggac gatcgatcac ttgatcgggt gagaatggca
     9241 cgtggatgaa cccacctcga attgccggga attgtgtttc gatcaaatga agcaaacgat
     9301 acatgatatc attgcagaca aatgttcctg ctgtataaga gatgtacgct ggtagaccat
     9361 gatcttgtac attttttgtc attgctttga ttggcaatga ggtaaagtaa gcggctggac
     9421 cgttttcttc taaagccgta tcgaatggct gatttccttc attatcaggg attcttgctt
     9481 ctgctagatt gattgcgaca cgttcgaacg agactgctga ccgcccacct gcttgtccga
     9541 cacagatcac gacatctggt tggtgctgtt ggatggcttc tttgactact tgactgcttt
     9601 tttcgaatac ggtcggtact tccactttga tgatttcagc accgctgatt tcatctggca
     9661 ttttttttac tgcttcatag gctggattca ctttgtcccc accaaatgga tcaaaacctg
     9721 ttactagtac tttcatctgt tgttcctccg tccgattgat ttaacatatg gttatctttt
     9781 atcataccat tttccttgaa cagcgtaaat aaaggaaaca atagtttaaa taacataaca
     9841 gatcaaactt aatatattct tataaaaaaa tagtgttcca ctacaatgtt agaagaggtt
     9901 gcgacaatta ttctgtcaca acccctctct tctattattt aaatagaatc atataagcaa
     9961 tttggaaaac tagcataaat aacgcaataa acaactggtt tttgatcact ccgtatttgt
    10021 ctttcatctc cagcatcgct acaggcacga tattgaagtt agcagccatc ggtgtcagca
    10081 gggtcccaca aaaaccgcaa gtcaatgcaa ccattcctat taacgctgga ttcgctccat
    10141 gactaaatac gaatggtgca ccgatcccaa ccgtcatcac ggtgatggcc gcaaatgcat
    10201 tccccatgat taccgtaaat atcaccatgc caagagcgta aatgatgatg ccgatgttca
    10261 catttccttc tggaacgatc gtacctactg cattggaaat cactgtccca actccagcgc
    10321 ttgtaaatac cgcacctaga gaagctaaaa gcattggcaa catgcttaac ggccctactg
    10381 tgcccagcat atctgctgca tcatctaaaa atgtacttgg tcgattatct cgagaaaaga
    10441 acatcaagat tagcatggct gcaaaaacac cgacgcccac gccgactaaa gcacctaaat
    10501 tggtaaatag agcaaacaag atcgcaaaga caccgatact caaagctgga ataaaaattt
    10561 tcattcctag tttgtctgac attttttcca tatattcttt tgatggaagc cggctttctc
    10621 ctttgctgac tttcttagca attgctggta tggtcatcgc aaagatcaag ccaccgttca
    10681 cgattgccgg aagccatctt cctcctgcaa taactacccc taagaccgtc cagaaaaagg
    10741 ctgacccata acgatgagga ttggttttat ctgttaaatt tcttactcca gtgtaaatca
    10801 atacgagtcc catcacgata taaaggatct ccagtaattt atcgcctaat aaaatatcag
    10861 gatttgtaaa aaagtccatc ttattgcccc tttcctggat aatatttctt cgctaatttc
    10921 ttttctttaa gatagaagta gacacttgcc acaactaaag ccgtgatagc cactggaact
    10981 tccactaaag caagctgtcc caatgttacg tcatatccta gatctttcag tgttgattgg
    11041 actagcaacg cccctgctcc tccgatgaat agtacttggc caaagaacca gcagacattt
    11101 tccatagctg aagccatacc ttttagttct tcttcatgct taggatggac ttctaaccct
    11161 tttgattcga tggtccccaa tgccatcggt aaaatgattg gacgaacaaa tccagcaact
    11221 ccaccaaaac tcacgttaaa agccgcaaag atcattcgaa agatcccata aatatctaca
    11281 atcataccag cagatacttt cttgaatcgc ttgatcaagg aagccgctga ttctttcaga
    11341 ccatttcttt caagcgttcc tgttgccaac ataatgatga taaaaatcgc catcccgcga
    11401 ttgtttacaa aacttgtacc taaaagttct aacattcctt gaatgccaag accgccagtc
    11461 aaagctgtaa caactaaagc aaccattacg atcaatatcg agtctaattt aaatgcaaac
    11521 ccgagaataa caatcaagac gcctaaaagg ctaagataat tttccatata ttttcctcca
    11581 taaaatgaca atattttttt aattataaag caaaacacat aaaaaatata gttgttttct
    11641 cacgaaatag acactatttt taaatatttt tcgttatttt tttatcacaa aaattatctt
    11701 acttattgta aggtaaagct ttttttaatt tattgcccga taatatatag aaataaggtc
    11761 tagttactgt tttttttaaa tgattaataa ataacaactt ctttctatga aattgcaaag
    11821 cattcatctt cttttccgct aacgatatat ccgtcagtgc ttggtacact ttactaaaaa
    11881 gtatgagatt gccgatatct ggataggtgt gctatactac agaaaaagat atacaatctg
    11941 ccaccgggta ggataaagag gatgatcatt ccattaggtc tttgaaggga tgattttcct
    12001 ctttttgtgt atttttttat agttaaggag gaatgtaagt atggcatgga tggaattaat
    12061 catagcagga attttggaag tattctggtc aacgatgatg aaatggagtg atggtttttc
    12121 taaaatcaat tattcttttt acacagtgat tggcatgatt gctagttttt actttttgtc
    12181 aaaggcaatc aaaagtttgc cgatgagtct ctcttatcct atctggacag ggattggcgc
    12241 agttggttcg gtcataattg gtgtggtctt gtttcatgat aagctgaatc tttccacttg
    12301 gttctttgtg gggatgctac tcatcagtat cattggcatc aagattacta gcggccacta
    12361 gacacttgga ataagtactg atcagaaaaa ataagccttt ttatttaata ttcaattttt
    12421 ttatgaaaaa ataagaaata agtccaatga cctagcgact tctccactca tcaggtgtat
    12481 gcttgtacta tctatagaaa aggagtgaat atttttgaaa agtctttcgg tccaagaatt
    12541 atccaagaac tttggtcaca aacaagccct cagcgatgtc agttttagct tatcccctgg
    12601 caaaatcgtt ggattagtag gtcctaatgg cgctggaaaa acaacgatca tgaaagctat
    12661 tttaggactg atccattact cagatggaac aatctgtatt ggtgaaaatg agatttctcc
    12721 ctctagtcat aaagggttgg aacaagtagg agcattgatc gaatacccgg gtatctatcc
    12781 attcttaaca ggctatgatc atctgaagtt attctctgaa accaacgatg tatctgctat
    12841 cgacactatc gtgaatcagc tcaagatgga aaagtacatt cacaaaaaag cgaaatccta
    12901 ctcgttggga atgaaacaaa aattgggaat tgctcttgcc ttactgaacc atccgtctat
    12961 cgttatttta gatgagccga tgaacggttt ggatccacaa gcaacaagag atgtgcgaaa
    13021 aatcattacc gacttggcta ctcagggcac ctctttcttg atctccagtc acttactcag
    13081 cgagcttgaa aaaatggtcg atgagctgat cctgatcaat caaggaaaga tcatcaaaca
    13141 atgtacaatg gctgaattga accaaacagc gcaagatttc atcgttatct ctacaacaaa
    13201 tgatctagct gctcgaacga tcttgacaga agctggatat actttggaag atcctgataa
    13261 aatcaagctt ttaaaagaaa acgaagaatt attaggaacc gtgatccagc tattgtctgc
    13321 acaaaatatc catgtgacag acgtccagca tctgacgaat gatttggaat cttctctatt
    13381 ggaaattctg gattcagtaa agggggaaga agaaaaatga cattgcttgt aaaacaagaa
    13441 ttatttaaat tgatcaagaa aaagtcaact gctgttttat ctgttctatt agttgttttg
    13501 ctcataggaa cagcacttct agccaaaaaa tacacgacga tcattgatcc agttgaaatg
    13561 actgctcagc tttttagtgc cacatcttgg attgtcttta tcatgattgc agctgcaagc
    13621 acgatcattt cgatggaagc acaatatggt acattgaaaa atctccttta tcgaaagtat
    13681 tctcgaggag aaatattagt aagcaaatgg atcacattag ttatttattc tgtttattta
    13741 taccttttag ctattattgt cactgtgttg atgaaactga ttcttttccc ttcgatttcc
    13801 ttcacggaga aggtttctac tggtcaaaca ctgatccaaa gcttgttcac ttatactttg
    13861 ggtagttata tcggtttatg gctgatttta agtttagtgt tgatgattgc ttgctttatc
    13921 aatagttccg gcgcatccat ctcagcagga atcgtctttt attttgcttc gtcaatcata
    13981 tctggtatcc ttattgcact catccaaaaa tgggagtgga tcaaatggaa tccgatcagt
    14041 atgttgaatc tgcaaaacca aattggaaac gaagagatca tgaagcaatt gactcatctt
    14101 tctactaatc aaatgctatt cggtaattta gcttacatcg ttttgttctt agcattagga
    14161 tatctcgtat ttaagcgaaa aaatgtatga tgactcatca aaaaagaggc tgtgaaagag
    14221 acgttcagcc ttattgccga ttggctgtct cttaaacacc gtttattctg aaaaaggagc
    14281 tgtgacaaca tttgtgtcac agctcctttc ctagtatgtt tttgaataaa cgacaggctc
    14341 acaacctctc tttatacttc ttttatactt ctatacgtga agcatcccag tggcaaataa
    14401 aaccattgca atcactccga ccatcgtcac acctataagt aaaatcttat ctaagtggat
    14461 atgttgtaca ggctcgtttt gctccttacg tgctttgagg taaaaataaa tgcctggcaa
    14521 gaaaagaaca agaacaagca ttacttcatt aaaggctacg actagcatag cccatacttg
    14581 aaaggcactt gctaaaagcc ctactaaaaa ttggacccat tctttccgct gataagaaag
    14641 tttcatttgg tacaatccta agaaaagcca cgtgaagaaa atagctgctg taccaagtgt
    14701 gtaagcaaag ttataagcac tgtcagtgaa taataatgaa agtaaaaaga cattcgtaca
    14761 taacgtcgtc aatacgatag cataagttgg tgccccattt ttgttgagtt ttccccatgc
    14821 tttaggcaaa agtccgtctt cagccatgtt tcccattgtt tcaatcggta agatcgtcca
    14881 tgagagccaa acaccaacta aagaaatgat caaaccgccg ttgatcacga ctgctcccca
    14941 tttaccaacc atcatttcaa acacatagcc catagcaggc tgcgccttga tcgtttgcag
    15001 ctgattcatc gtcattgtcc catatggaag catggaaacc aagacgtaaa taataatcaa
    15061 gccgaatatc cctagaatcg ttgctttccc aacatctgat tgtttttttg ctcgtgaacc
    15121 tacgacagag gctccttcaa taccaacaaa tacccaaagc atcaccatga tgcaactcaa
    15181 tacttgactg gaaaacggga tcggctgtcc attcgcattc aatataatgt cgctagaaaa
    15241 gttatcaatg aattgttgga tcgaaaacgt gctgatagct actacaataa ataaaaataa
    15301 tggcactaat ttacagaatg tgacgatagc attgacgaaa ctagcatttt cgactccccg
    15361 attcactagc catgcatatc cccataaaaa gactgtactc atcacgatcg atgccagatt
    15421 ttgcccacct tcaaatactg ggaagaaata tcctaatgcg ctcatcaaca atgttgcaaa
    15481 agctacgttg cctaaccagc atgtgagcca gtaaccccaa ccgctgataa aaccagcgaa
    15541 acggccaaaa ccttcttctg cgtaactaaa gatcccgcct tttaaatctg gacgtttttt
    15601 attgatatta ttgatcgaca aggagagcat caacattgct gttcctacga gtacccaact
    15661 gaaaatagct gggccgccag ccgatccgga gatgtccgtc ataataccaa atacgccacc
    15721 tccaatcgct gaaccaatta ctaaggcagc aagtggaatt aaaccaattc cttttttctg
    15781 ttgctctgta tccatgtaag catcctccaa aataaaaccg cttttatcaa aaagtataac
    15841 aaaccttttt tatttttact taattaataa aaactttaat agaaatataa ttctaccggc
    15901 ttttttctca ttatacaagc aaagcgccta gtccctttcc aaattacacc accaaaaaac
    15961 ttaaaaaaga tgctatacta aaaggaaaga attggaggat gatccatgaa cattttatat
    16021 atatcgattt caggaaatac gcgtgccttt gccaaacatc tagcagaata tgctgaaaaa
    16081 atgcatgcag aagatcctgt caatcctgaa gttactttaa aagaaatcca tgaaaatagt
    16141 gattttacga aagaaacaga accatttttt actttcgttc ctacttattt agacggcgga
    16201 aatggtttag ataacggtga tacagaaatc ttgaccgaaa cgatgcgtga atatctggaa
    16261 catgaagaca atcataaact ttgtcttggt gtagtcggta gcggaaataa aaacttcaat
    16321 aatcagtatt gtctaacagc caaacaatat gcacaaacat ttggctttcc tttcttggca
    16381 gattatgaat tacggggaac accatctgat gtgacacgaa tttatcagat tttagcagaa
    16441 aacaataaat aataaaaaag aagaagctga aaaatagatt tcagtttctt ctttcgtttc
    16501 tattgtttct atcatattaa gctttgacat ccatcacagg tgcttttgct tttgctttac
    16561 ctgttttttc caagacaaag ttttttacac gatccatatt ggttaccaag acgatcatgg
    16621 atgtttcttt gcctgctgct ttgatcgctt ctaagtcagc ttctgctaca agcgtatcag
    16681 gtgtcacttg ctgcccttct gttactttca catcaaatgg cgtaccgcct aagtcaactg
    16741 tgttgattcc catatgaagc aagatttcta aaccatttgg cgttgtgatc ccaatagcgt
    16801 gtttcgttgg gaagacagag ctgactaccc cagtaactgg agaatagata tgtccgtttt
    16861 ctggttctac aaagtagcct tcacccatca tcttttgtgc aaaaactgga tcacttgctt
    16921 tatctaaaga aacaactgtt ccgtcagcaa ctgatgagag gatagtcaca ctttcagctg
    16981 aagtttgagg tactactttt tcaggcactt catttactgt ggctgtttca gtcgctaatt
    17041 cttctttttc taaacggtct ttaggaatac cgaagaagaa tgtagcaacg aagccaccag
    17101 cataagctgc caataatcca aggatgtagc ctaaccattt accgtcagag atcaatggga
    17161 tcaaagcggc tcctgaaggt ccaatagcga tagccccgac atttccgatc attccgatca
    17221 ctgccccgcc gatacctcca ccgatacaag cagtaataaa tgggcgtcct agaggtaatg
    17281 ttactccgta aatcaatggt tcgccgattc ctaggatccc gactggtaaa gctcctttga
    17341 tcatttctgt taattcttta tcttttttgc agcgaatcca taatgccaat gctgcaccca
    17401 cttgtcctgc accagccatc gcaagaattg gaagcaatag cgtacgacca gtttcatcaa
    17461 tcatttgcac atggattgga gtcaaaattt gatgcagtcc gaacataacc atcggcaaga
    17521 ataatgtgcc tagaacgaag ccggcaaatg ctccaccaac gccaagtacc cagttgatcc
    17581 caccgaccat gccatcagaa atgaaacctg ctaaaggcat gatgaagaaa atttcaatca
    17641 gaccgatggc aatcaatgaa agagttggtg taacgatgat atctaccgca tcaggtacta
    17701 ctttatgcag ttttttctca acgattgaca atagccaaac agcgaagatg acacctagga
    17761 tcccaccttg tccagctgca agagcttcac cagtaaataa gtttttcagt ggtgcttccg
    17821 gattcatccc tgtcagcatc acaacggccc cgataactcc accaagagtt ggtgttgcac
    17881 caaaaacttg tgcagcattg atacctgtat agataactag gtagctaaac atcccgtttt
    17941 tcaagatgtt catcacatcg atatattgtt gccatgttgc tgcatcaagt gttccggctg
    18001 tgactaagtt tgctaaaact gcggcaatcc ctgccacgat cccagtaccg acaaatgcag
    18061 gaatcattgg cacaaagata ttagaaatgt cttttaatac cgatttgatt ttagatggtt
    18121 tttgtttcga tttttgtgcc gctttcattt cttgtgcccg ttcgtttaca cggtcttttc
    18181 cagatgctga tgaatttgct gtagctgtac ttacagaaga aagtttttct cctaatttca
    18241 cgcctgccaa atcaaccatt tgctgtgcta ctttgttcac tttcccaggt ccgatgatga
    18301 cttgaagctg ttcatcttct actacaccaa gcactcctgg aatcgctttt aattcagaga
    18361 catttacaag atcatcattg atgatcttca tacgcacacg agtcatgcag tggacaatgc
    18421 tttctacatt gcccatgccg cctgcccctt tataaatttg ttttgccagt tcttccaaag
    18481 tcagttcgtt ttttgccatg gtcttttccc cctcataagg tcccttttac aaatccgttt
    18541 gcttcagcta attttgcttg agcttcctct ttatcactat ttgtcaaaat catgacgata
    18601 gccagcttaa cattttgatc tgcttcttca aagtactttt cagctgtttc gtaatcagct
    18661 tctgtcgctt gcatgatgat tcgttttgag cgttcgatta gtttttcatt tgttggtttg
    18721 acatcgacca tcaagttatt gaatacttta cctataccaa tcatactgat agtagaaatc
    18781 atattcaaga taagtttttg tgctgttcct gacttcaatc gagtagagcc tgtcaaaaat
    18841 tctggacctg catcgacttc gattgggaaa tccgcgtgtt tactgatttc ggcttgttta
    18901 ttgcaagaaa tcgttcctgt gacagcaccg atcgattttg cgtaatctaa tccaccgatc
    18961 acatatggtg tacgaccgct agctgcgata ccaataacta tatcattttt tgtcaaattc
    19021 agcttgatta gatcttcttg ccctagctca taagaatctt cggccccttc gacagctaca
    19081 gtcattgctt cttctccacc agcaattaga cctatgacca tttccggttc tacaccgaat
    19141 gtcggtacgc attcagcagc atctaacaca cctagtcttc cacttgttcc ggctcccata
    19201 taaatcagac gtccaccttt tttaaaggac tcaatcgtgt tagcaatcac tggttcgata
    19261 cttgtcagtt ctttttcaac cgcagcggcc actttctgat cttctttgtt cattagttga
    19321 acggcttgag aaatactcat ctcatctaat ccaaaagttt cttggtttct gcgttccgtt
    19381 gttaggttct ctaaattgat tttgctcatt tttatcttcc ttcttttctt ataaattgat
    19441 agttcatacc agctttgatt tggttgatca atggtagctc ttctttgata actttagcta
    19501 ctacattcac tttttcatcc gctggcaaat catatttggt taattggatc tcacccatat
    19561 agcgcaaata cttttcgtta tctaaagtaa tagacccttt catacgcgta gctgtatttc
    19621 ttgctgggat aggcggaatc tctcgaaaac gtgcatctac gcttcgtatg acatctcttg
    19681 catcgtcttg ccgattcgta tgcttcccta atatatattc gtaaaattct tcatctacag
    19741 gttccgtatg gagcaagact gtctgttctt tttggaaaga agcaaattgc tcctgaacgt
    19801 tttctgacag acctgcatct ccgatgtaaa caatatccgt gttacttgct aatagatcta
    19861 gagcgcctgc aagaggatgg atccctcgat gttcttccaa agttggcaat ccttgatata
    19921 gtgggcctct caactctgta tctcctggta caaaaccttg gacaaggaga ccttgaattt
    19981 tgagccattg attttttttc tgataccatt ctttgtccag ccctgtttcg ggacgtggat
    20041 aataattatg ccaagcttca atctgtgaga aatcagcttc tgcttctttc aattcatcga
    20101 tatctttagg cgtgatcgtg ctagcattca aactgatttt caattgatgt gaccagtcgg
    20161 caatctgctg attggaaata tgataatcca ttcttaaacc tgttacacca attgctttca
    20221 aaggcgttag atcttctaat gaaaaacccg ctcgttccaa tgcctctcct gaaatatcga
    20281 ccatcaattc tagtttattc tttttcgctt cgcttcctag ttcgatcaat cgcttttgat
    20341 atgccgaaac atcatcttct ggtatatgca tggaagtgaa aataccgaca aagccattgg
    20401 atgccatttg gcggatatac tgtttcttct cttctgacaa agaatcgttc ataaatattg
    20461 aaaagccaaa catattatcc ctctttttct gtatccgtct tcatttctat gactagagta
    20521 tatatctaaa gaaacaaaat ttcaatactt ttattaaaat aattaaattt catttctaaa
    20581 ggttgagttt atattatcaa aagaattgtc ataacctaaa aaaggcactg ctaagcaaaa
    20641 gcagtgcctt gaaatatttt atttaaataa cacaaaaaca taattactca taagaaacca
    20701 attctgtatt atctaccatg aatgtaaaga acccctcatt aggcatcgga tgaaaataag
    20761 cattgatcac tcttatcaag gcattatggc aaactaacag atagacattt tcttcgtctt
    20821 cgatgcattc tttcaataaa ggaacgatcc gtgcatacac atctaagact gactccccat
    20881 ttgggaatcg tacagcgaac gctaaacgtg ctttttgaaa gttctcatct ttgcttggca
    20941 gtccatcata atccccaaaa tccatttcta ccaatcgttc atcaacggtc agcggcaagc
    21001 tgagacgatc agcaactgct tgtgctgtat ctcttgctcg ttgaagaggg gaatggatga
    21061 ttttcgtgat tggaacaggc aaatctacta ccttttctgc tagtttctgt gcctgcatct
    21121 gtcctttttc agtcagcggc acatctgccc gtccgcaaac taatccttgt gcgttccaag
    21181 ttgtttctcc gtgtctcgtt acataaagca tgtgattctc tccttcgttg gcaaagttcc
    21241 ccttaaccat accttgttgt tcttgagatt acaagggtta gattctctca agagaaataa
    21301 tctcagtaga tactttatcc aggaaggttt gatcatgttc cactagtaac atggcaggtt
    21361 tcacgttcaa gatcagttgt tctaattgtt cttgattgaa gacatccaaa taattcaatg
    21421 gttcatccca tgtatatagt tcagctggct gtgataaaga tttagccaat tccacttttt
    21481 tccgttggcc catactcatc tgctcgatct tgttatgaaa aacatctctt tccatcccaa
    21541 gctttcggag gttgttcaaa aatgcttggt agtctacttg gtttttctct gcaaattccg
    21601 ccaacgttcc tcgattgtct tcataatttt ggcttgcata actaatgctc agatgttttg
    21661 gctggtattt ttctcctata accttgccgt tgaatgcccc cagaagataa tggataatgg
    21721 atgactttcc tgcaccgtta ggacctgaaa tcgccacccg ctgatgaggc tcgattgtaa
    21781 aatgaattgg ctcgaataac agattttcat accctaattg aagatcttct acgcttaaaa
    21841 gtcgcttatg gtgagacgct tggctattca tcgtcaaaga atcgatatat tcgatatctt
    21901 ttaatagttt ttctttctcg ctgatctggg tctccatccg attcacgatt gctttggatc
    21961 gtttcatcgt ccgtgcagca tcagcaccca ctgctccttt attcactcgt ctagattcag
    22021 tatcgatgaa tccgacttgt ttctttgttt tatctccttc tcgggaacga gaccattcgg
    22081 ctttttcagc tgctgttttc tttagcctac tgacttcttt tttcaatttt tcattttgag
    22141 ccatttcaaa ttcatcacga agttttttct gttcttcata gatagagaaa ttcccttgat
    22201 aaagttccag ttgacttttt tcgattgcta aaacatggtc cactacttca tcgataaatc
    22261 cccggtcatg gctgaccacg atgaagcctt gttttttctt tttcaaataa gccgctactt
    22321 gttttctacc agagatatcc aaatgattcg ttggttcatc gattaacggg aaatgagtgt
    22381 catccacaaa taaaagtgcc agtaggactt ttgtcttctc tccccccgat agtgtgctga
    22441 attctctcca taagatttca ggatctgttt gcatcaattg gagctctctt tcgatttccc
    22501 atatctcaaa atccgtaata tcatttaaca cgtaataggt taaacgttct ttatcttttg
    22561 tctgttgcgg gaaataggca aattcttgct gatggattac ttgcccttga taaggtagtt
    22621 tgttttgtag aatattcagt aaagttgtct ttcctcgacc gtttcgtccg ataagtccta
    22681 gtttccattg tgtgtcaaaa tttagatttg cttgttcaaa taataatgtg ccttggctgt
    22741 cgtagccgaa tgtcagattt tttatttcga ttttagacat tttccatcac ctcgtaaaaa
    22801 aagaccagtc ctattttcag aactggctaa agaggatgca agggttggat acacacaaaa
    22861 agacccatac atcgaaaaaa ccaagccaat cccgaaaatc tgcacactgc ttccttgtct
    22921 ccacaaggta cagtctgaat gcagctttca tcaatcgact taatttttca atgcacggat
    22981 ccctcacttt gtcctatatt tgaaattacg atagcataaa gaaaatgatt ttgtcaacta
    23041 cttcgggagg atggtgaaaa atctgctaag tgtctgtcct aagatttttg ttttttctaa
    23101 tgtttgcctg tgttccattg cttcttctaa acctattgga cccaaatgaa tacttaagac
    23161 aattgggaga aaatcagaaa gtccttctag ctcttggtct ctacttccgc aaatagcgat
    23221 tattggtttt cgatattttt ttgcaagcac tgctacaccg tatggaactt ttccgtggat
    23281 cgtttgctga tcgatccgcc cttctcctgt aatgatgaca tctgcttgtt cgatcttttt
    23341 ttccaatcca gtgatttggc tgactaaatc gaaaccagaa accatcttcg ctcccaacaa
    23401 aaaaagggct ccaccaatac cgcctgctgc tccacttcct ggaatatcat ctaaatccac
    23461 ctcccagaaa tcttttattt gctgagcaat ctttcctgct tgtttatcta agaaagctag
    23521 cgtcgtatct attcctcctt tttgtcttcc aaattgccaa gcagctcctt gttgacctgt
    23581 atatggattg gtaacatctg cagcgataat caattttact ttagaaacaa gaacgtttct
    23641 tcgatcaaaa tgaagttttt tagtagataa taaaggattt ccttctgtgt acccttctaa
    23701 agaagcaccc aaggattgga gtaatcctag accaccatcg ctacacccgc ttcctcctaa
    23761 agtgacaata atttgttcta cttcttgaag cagtgcatct ttaataagtt ctcctaaggc
    23821 ataactggaa gcaagtcgga tagtttgatc cgttggttta tcaagtagat ctaaccccaa
    23881 tgcttgtgca gattcgatga ctgcggtgtt ttttccttgc caagttgtca ataaatagct
    23941 gatttttcgc tgacggaata agagatccat cccatcaatg gtacgccatg ttcccctgag
    24001 cgcgtaatag attgcttctg cagtattttc tccgccatct gcaatcggca cattgatttt
    24061 tcttccttta cgatcttccc agccatttag aactgcctga tttagttcaa atgaagaagc
    24121 acttccttta aatgaatcaa ttgccgccaa aatatccatc tgtatccctc actttcttct
    24181 taagtaagac actttttttc atttttttca aacaaaacaa tgctattttc ttccattttt
    24241 ctatatacta aaagggaact cattgaataa aggagagaaa aaacatgaaa atcgcaatag
    24301 ctggggccgg agcaatgggc agccgaatcg gattgatgct acatcaaagc ggaaatgagg
    24361 tcctcttgat cgatcgttgg cctgctcaca ttgaagccat tcgtacaaat gggttgattg
    24421 ctgactttaa cggtaaagaa gtggtcgcaa aactgccaat ctattcacca gaagaaatca
    24481 tcgaaagcaa cgaacacgtg gacttgatcg tagcattaac aaaagccaat caattagatg
    24541 atatgttttg ttcgatccaa tccattatta ctgacaatac ttatgttttg tgtttactaa
    24601 atggattagg tcatgaagat gtactggaaa aatatgtgcc aaagaaaaat atcttatttg
    24661 ggatcacgat gtggacagct ggtctagctg gtcccggaaa agtcacattg ttaggtgatg
    24721 gagaaatcga actagagaac ttggaaccag aaggcgaagc atttacaaaa aaagttgtag
    24781 aagtctttca agaagcaaat ttaaatccga tctatagcca taacgttcgt tattccattt
    24841 ggcgaaaagc ttgtgtaaat ggaacattga atggtttatg cacgattttg gattgtaata
    24901 ttgctgaact aggcgcgcaa aaagcggcag agagcatggt tcgaacaatc gtatctgaat
    24961 ttgcttctat cgcagcaaaa gaaggaatca tactagatca agaagaagtg taccaacata
    25021 ttgcttctac ttatgatcct gacaacatcg ggttgcatta cccatctatg taccaagacc
    25081 tgatcaaaaa ccatcgtttg actgaaatcg actacatcaa tggtgccatt tggcgaaaag
    25141 gacaaaaata tgatattgct acaccatatt gtgccttttt gacccaatta gtccatgcaa
    25201 aagaaggaat ccttggcgca gaataaaagg ctctctgatg agaatcagct gaatcagcag
    25261 ctggttctct ttttttaagc ccaataaaag atcaaaaaga aaacacaact ctcatgatgc
    25321 cataagaatt gtgcttgttt gtttcagtac ataatctctt caaaatgtag gttttttatt
    25381 tgttttcaac agaggagagg tcatattcgt attgctcata aatagtggaa tcgttagtga
    25441 taaatcgttt tgcccaattt tctgcatcat aaaattccaa tagctctgat aggattggct
    25501 taaatggtgt atgcagaaat tccggtaatt catctaaatc acgttctcga agcaaaaaaa
    25561 tattttcagg acgataaaaa tgataatgag caatcgcttg attatccgtc actagtttct
    25621 tttgaaaata catggattca aatgtccgca aagtcactcc ttgctgaccc tcccgcaaca
    25681 gttccagaat cccgcgcgat tcccctgttt ttttcaggta ctcttcataa gtaagatatc
    25741 caggttgttc attccctcga tgtggaagaa tgaaatagtt ggtcgtaaca tctcttttta
    25801 taaattcctg cttatagctt tctgctcgtt cttttctccc attattcgtt gcaccaaaaa
    25861 atagatccgt ctttggttcg ctagaagaaa aattcatatg tttcgaataa aatgaactat
    25921 tatgtttcag cccaagttct tttgcttcca aaaaatcaaa atgataaatc tcatcgattg
    25981 ccggttcatc ttgtgcctgc ttcagcatcg cctcgtgttc tgctttgaag tgattccaaa
    26041 aaaataacac aatttttgca ttggtaaatg ggcgcacgcg tcgagccatc tccatatcaa
    26101 atccataatc taaaaaaacg atcagctgat aatttgccaa ctgttgtcta gctgatttaa
    26161 tgaattccga ctgtagtaaa tcttttccgg accaaacatc taaagagcgg atggaaggca
    26221 aaaaataaag tgatttattt tttgcaacta gcagtacgtc atttttgttc atcttttccg
    26281 cctcactttc tctgtcactt tttcctatga tagcctgttt aaatagtaaa aagccagtaa
    26341 aacgtttcct atatttgtat gaaaatttaa catttgtatg aaaatatgat gaaataaatc
    26401 attcttctat taaaattcgc ctaaaagttt tctgaatagt tgtaattgtc tgaaaatttg
    26461 ttatcattag tcattgttaa atattgctat ttattcttaa tataaatttt gattgtgaat
    26521 ttaaaagaaa gaggtcattt atggaagata aaaaattgaa aaaacaactt agctccagac
    26581 atatcaccat gttagcttta ggtggagcaa tcggtgcagg tctatttaaa ggaagtggcg
    26641 aagcgattgg gatcgccggc ccttctgtat tgattgcttt tttgattggt gggctgattt
    26701 tatatatcgt gatgaaaggt ctgggaaaaa tcgttttaag cggtggcgac acgcatcacg
    26761 gattatctgg tttgatacgc ccttatctag gtgctcattc tgctgatttt actgattggg
    26821 tctactggtc tatgtggatt atcaatatca tcgctgaagc tgttgcagct gcttcctttt
    26881 tgcagttgtg gttcccgcac attccagcct gggtatttgt ttttatgttg gctgttttaa
    26941 ctacgatcat caatctttat tcggtacggt tattcgcaga aacagaatat tggctggctt
    27001 ttgctaaaat cagtgtcatt atcttactga tcatttttgc tacttatctt gtcggtcagc
    27061 aaatgttggg cacaggcgtt ttccctaccc ttcagcaatt gactgaccat ggtgggttta
    27121 ctcctcatgg tatgaaggga atcatcagtt ctttgcttgt agttatctat tcttacggcg
    27181 gttctgaact gattgctatc acggtaagcg aagcagatga tcctaaacgt gcgattccta
    27241 aagcaatcaa aggtgtcatg ggaagaatca tttcctttta catcattcct ttgtttttgc
    27301 ttttgattct ttttccttgg aatactttag caggcacgaa tgtcagtcct tttgtgatgg
    27361 tatttgaaaa aatgaatatc ccatttgcag cggatatcgt caattttgtg attgtccttg
    27421 ctttgttctc atctatcaat tcgggtgtgt atgcatcttc aagaattttg ttcttccgtt
    27481 tgaaggatcg aaaaggttcc tctagaaaac tagctgtttt aaacaagcat caagtaccgc
    27541 aacgtgctgt tttattttgt accagtgttc tttatcttgg tgtagctctt tcttattttg
    27601 tcggtgacaa attatttggc tatctggcag gttccctttc gtatacagta ttattgatct
    27661 ggatcattat cagtgcggca tcctttgtac tggcactaag aaaaggaaca ttatgggata
    27721 aaggaatcag cttttttgca ttagctgtct taggattgat tttccttggt atcctgttca
    27781 cgaactccat tggggtaact gtattgactg gcctgcttta cctgttcatt tatttcagtt
    27841 atcaaaaaaa gaatgatgcg ttcgtattga cgaatgaatg aaatcgatag aaaattttat
    27901 tttatttatg tggaatagat ggagctattg aagaaaggag ttgtgacaaa agtcttgttg
    27961 caactccttt ttccgaataa acagtctcat gacctcttgg taaacggtgt tcaaaaaccc
    28021 tctgtgcaaa ataattgatt gttctccatg ctcatgacat gatagaagaa aggaatgaaa
    28081 aaaaggagtt tgttttaaat gaaaaagtat tggaagtttt tgttgatgat cggtgtttca
    28141 acggtagtta tgtttggtat gatgtacttg aatgtctatg cgctcgatca tatcttcttc
    28201 agtcgtacac gagtattcat ggcgttgatg atgggtgcag tcatggcagt catcatgctg
    28261 ttgttcatgt ggaaaatgta tgaaaacaaa aagataaatg ttatgatctt agccgctagt
    28321 cttcttgttt ttgccggttc cttgtttatg gtacgaagcc aaactactgt caacgacaca
    28381 gcttggatga aggccatgat tcctcatcat tctatcgcta tcctggcaag taaacgagct
    28441 gatttaaaag atcctgaagt aaaaaaatta gcggaagaaa tcattcaggc acaagaagaa
    28501 gagatcgaca aaatgaaaaa aatgatcaaa gaaatggaat gaccattatc cgtccaaata
    28561 aaaaaagagg atgttccaga agttatcagc ttcaagaaat aagaagaagc tacttctgga
    28621 acgccgtgaa taaggggttg tgaaaaagct gccctgcatc aagtaataag aacagccaaa
    28681 caaaaatagc ttctcctatt tttcgtttgg ttaggcttat taccgcagat gcaagctttt
    28741 gaacaccgtt tattaagagg ttgtaaaatc agcgttctga cctgagtatt agagagacaa
    28801 atggaaaaat agttcctcat attttgccat tttttgagct aataccgagg gtcagctgat
    28861 tgaacaccgt ttatcgagag gttgtgaaaa agtcgcttag tttcgcgtat taagaaaaat
    28921 tttgtgaaat ggctttccac atttttgtat aaatttggct taataccgag aaactggctt
    28981 ttgaataccg tttattcgta aaagggattg tgacaaaagt cttgtcacaa tcccttttta
    29041 tgtttttatt gttctaattg cgctgtcttg cttactgctt cttgcggttt cacaaatttt
    29101 tttgagattc cccaacctgt gaaggcactg accatcgaaa acacaggaga aagcaaactg
    29161 aaaaatacga atggcaagta agtgagtgta ggaataccaa atgtacttgc tacgaaactt
    29221 ccggcaattc cccaaggcac taagtagttg attactgttc caccatcttc tagcgttcta
    29281 cctaatacag ttggatcaaa tcctgctttt tcataagttt ctttaaatgc attaccaggc
    29341 agaataacag ataggaattg ttcaccaata aaaatattta ctccgatact tgtcatcaat
    29401 gttgtaaaaa tcaaatttgg tgtgttcgtc atctttcttg atactcgttc catcacggtt
    29461 gagatcaagc caaattcgat caacaatcca cctaatgaca aagttagaat aatcaatgac
    29521 acagtaggca tcatactttc gattcctccg cggctaagca gtgaatcgat ttgttggttt
    29581 ccagtatttg aaacaaaccc tgattcgatg accccagcca acttcgttaa ggaagtttgt
    29641 gggttttgaa taaaaatcat tcctactgct acagcgatat tcaacaaaat cgttaaaatt
    29701 gccggcacct ttttccatgc acacagtaac attaaaaata aaggaatcag tgaccagatt
    29761 gaaatagtaa agtttgcttc tagaacatca gtcacttcac gaatagatga aagacttgcg
    29821 gctttacttg tgtgtcctaa aatcatgaat aaaaagaaag aaacaacaaa tgccgggaca
    29881 gttgaccaca tcatatgctt gatgtgtttg aacaaatcgg catcaacaat agctgctgct
    29941 agatttgttg attcggataa tggagacatc ttatctccaa aaacggcacc tgaaacaata
    30001 gctccaacaa ccagcgctgg atttaagcct agtgttgtcc caataccgaa aaatgcaatc
    30061 ccaatcgttg acataacagt aaaggcactc cctacagcgc tacctacgat tgcacacaca
    30121 acaaatacag aagggacaaa ccactggaca ctgatcaatt tgaacccaat gaccattaga
    30181 gttggaatga tccctgcttg tatccataca gcaatcaatg aaccgactaa aataaaaata
    30241 aagattggga tgattcctgg tttgatgcca ttgatgattc cttcgtgcag ccaatctgtc
    30301 gggtatcttt ttatcactgc atacaacatg accattccga tcgctgctaa gatcgtgata
    30361 ttaggtgaga cctttaatcc gacaacgcca atcgcaatgc ctaaaacaat aaccattaat
    30421 acaagtgttg cttctaaaaa acttggtgtt cctttttttc ccatgataat ctctcctttt
    30481 ttagttgttc ttatgttcta aaaaagttaa gatatcggat tactccgccg caattcatcc
    30541 gcgattcctc ctttataaaa ataaaaagac ctgttttaaa ctaaggacga atgactccgc
    30601 ggtaccacct taattaaaaa cagatctgtt tttcactttc aaacttttat tcaaatgtat
    30661 ggactttcca tgtaattcgt caattaggct tgatcggttc acagcgacca ccgactttct
    30721 gaacaaaaca cctaaccgct actgcgtgaa aagttgactt gtacgaagtt taccttccat
    30781 aagatcattt gtcaatgaaa aagaaaatat tttttcattt ttctgaaaat tatttttata
    30841 actttcacac acaagtttat tttttactag aaagtagcgc cctcttgttt ttttcttgct
    30901 actatttctt gatgttcttg cttgattctt tctaatagct ttggctcttt caacagatca
    30961 agagctgtat tggcgagtag ttctgctgca atcgcaatag aagcaaggcc tttgtcactt
    31021 ttagcagcag ctttgaattc ttctgtgtgt ccggcaatat atgtctcaga gattgctacc
    31081 gtaggctgga ttgttggtat gacttggctg acatttccta catcagaaga accgagactt
    31141 gttctttgtt ccgtttctat ttcttcatct ggaacacctg cttcttttgc atgctggaag
    31201 aataagtcat caaaagaagg agtaacgatg acatcatcaa ctgcattttg gaacaatcca
    31261 aactcaaaag tcgtgcccgt acttatcgca gcacctcgta cgatattttc cactttttca
    31321 tatactttat ctaatgtttg ccgatttcct gcacgtaaat agaaacgtcc agcagcgtaa
    31381 tctggaatga cgttggcagc tactcctccg tctgtaatga tcccatggat attgacatct
    31441 tttggcaatt gcaagcgtag agcgttgatt ccattaaaca cttggatcaa tgcttccaaa
    31501 gcatttactc ctttttcagg tgctgctgct gcatgggaag cgacaccgta aaatttgatg
    31561 tcgactggat cattcgctaa agattctgtc gttttcccat aacgaaaagc tggatggacg
    31621 cataacgcta catcgacatc ctcaaaaaaa ccttctcgaa caaagctgcc tttagctgat
    31681 ccgttctcgc ccccttcttc ccctggggtg ccatatacac ggatttctcc accagtttca
    31741 tcaatgaatt gcttcactat gctggcggct aatacggagt aagtaccaaa caaattatgc
    31801 ccgcatgcat gaccaatacc tggtaaagca tcgaattctg ccaaaaaggc aattgtggga
    31861 ccgggtttat tggaaatata acgtgcatca aaggctgttc ggtgtccagc tacttctttt
    31921 tctacccaaa acctttcttt ttctaattgt ttaatcaacg ctgctgagga ataaaactca
    31981 tagttactta cttcaggatg ttcgtggata tctagagcaa gtgcttgata ttctggtaaa
    32041 cgatattgga ttagatcctt caattggttt gttgagattg ttgttgtcat aaaaaattcc
    32101 tcctatgcta gtcatttttc ttattttctc ccgaatgttt cccatgcagg cacgcttgat
    32161 ccttttgatg ttttctcgat tactttgact gtgtcttctg tttgataagc gtctactatt
    32221 ttttgatacg ttttattgtt ttcatcttct tttcttgcaa cgatgatatt cacataagga
    32281 cgtgcatggt catccactgg ttctaagaag atggcatcag aagcaggatt aaatccagca
    32341 tctacagcta caccgctatt gatcactgaa atgtctacat ctgacaaagc acgtgctgtt
    32401 tgggaagcat ctaattcaga aatgtctaga ttttttctat tctcggtaat atcagaaaca
    32461 gtgggcgctt gtccttttgc cttatccact ttgatcaatc cagcagtttg caataacaga
    32521 agagctctgc cgccatttgt aacatcatta gggattgcta cttttgctcc gtctttgata
    32581 tcagctacat ccttcaaagt gttagaataa attcccagcg gtgcattgac agtatttccg
    32641 atcgatacta aatcagttcc gtgttcttta ttgtaattgt ctaaaaagaa ttgatgttgg
    32701 aaagaattca aatcaatcga tccatctgct aaagccacat ttggttgatt gtaatctgta
    32761 aatttcacgt attccaaatt aatgttctcc tttttcaaac ggtctttcac gttgtcccat
    32821 acatctgtat cttctccaat aatccctagt tttacgatag tttctttttt gtcagttgta
    32881 tctgcttttc cttggctaca ccctgccaac caaataattg ctacaaatgt aatgactaca
    32941 aaacgaataa tttttttcat tttttctcct ccactttcaa tgtgtaattt ttttaatcag
    33001 ttgattactg ataaattgac ttaaaaaaac taagattaga atcaataatg tagccacaat
    33061 cgttacatct gtttggaatc gattataccc tctcgatata gcaagattcc ctaatccgcc
    33121 tgcacctact gctccagcca tggtagtcag tccgatcacg ttgatgatag tcaacgccga
    33181 gacccgaatg ataccaggca aaccttctaa caaatacaca cgtaaaatga taccgattgg
    33241 actggttccc atcgcttctg ccgcttcgat caccccagga tcaacttcta gcagtgcgtt
    33301 ctcgatttga cgagcaaaaa acggaacaac tccaaaaatc aatggcacta aagctgcttt
    33361 cgcaccaatc gtcgttccaa ctaagaaacg agtagtcaat gctaaaagag aaagcaagat
    33421 aatgaaaggg atggaacgaa tgatattgat cagcttatca agtatttcaa atagcagacg
    33481 gttagctagt aatccattag ggcgtgtcac tactaacgtc acacctaaga tgattccaat
    33541 catcccgcca atgaatgctg tccaaaaaga catatatagt gtttgcttcg tcgcttcgac
    33601 aaattcatcc ggaatcaaat aaacattggg caaatatgtt ttaaagaaat cagtcatttt
    33661 atcttcctcc ttcgatcact ttgatacgac tatctaaatc gtttttttgt aaaatattta
    33721 tttttacacc agatttgatc aaataatcca atgctttttt ccgctgtttg gtttctccag
    33781 ctaaagtaac gatcaaactc cctaacggcg tttcttgtaa taactctaca ttgccatata
    33841 aaatattcgc tgttacttga tatttgctgt acagatgagc gattagaggt tcgttcgtct
    33901 gatttccaat ataacttaat tccaccagcc atttattggc gatctgatcg gcaaaagcag
    33961 aatgggcaat gatcgtttct aatgcttggt caagatgagt agctgttcga ataaaatctt
    34021 tggtcaattc ttcttcagga ttgctgaaga tctggatgct gctacctttt tcaacgattc
    34081 ttccatcttc cataaccgct accttgttgc agatttcttt gacaacttgc atctcatgag
    34141 tgatcaggac gattgttaaa cctaactggc gattcaattt ttttaacaat gcaaggatct
    34201 gaagggtcgt tttcggatca agtgcactgg ttgcttcatc acataataat accttaggtt
    34261 cattcgccaa agctcgtgcg attgctactc gttgtttttg tccccccgac agttgtttag
    34321 gataagcaga tgctttttct tccaaaccga caagttccag tagttcattc actttttgac
    34381 gacgttcctg tttcgatttt ccagaatact ttaaagaaaa atcaacgttg tcaaaaatag
    34441 ttcggctttc cattagattg aaatgctgaa agatcatacc gatggccttt ctttcttttc
    34501 gcaattcttt gggagagagc tgactcagat tcgtctgatt gacaatgact tctcctgctg
    34561 ttggtttctg cagcaaattc atcacacgaa ctaacgtact tttccctgca cctgagtaac
    34621 cgacgattcc ataaacatcg cctttttcaa tcgttaaatc tacagaatct actgcctgta
    34681 tctctttttc tttttgtttg aaacatacag aaacatctcg caattcaatc attaaaattc
    34741 ctccttatct aaaaaaagcg tcctcaagaa aatgaaaatg tcattttctt gaggacgctt
    34801 ttttgcgtgt taccacctca tgttcaagct gtgttcacac acaactcctc ttcaagtacg
    34861 atcatactct agcaagatat cggtcgcagc cgtcatcgcc ttacatctat gctcgggatg
    34921 aattgctcga tggccatttt ccaatagtcg ttccttgctt cttctcagct accgaagctc
    34981 tctgtcaaag gttcttctat cgtacttacc atttcaatgc aaataatatt caaaaaaaaa
    35041 gtccttttac taacataaac tatgtcagta aaaggacgac gtatcgcggt accacctttt
    35101 ttcggaagtt tccttccctc aagcccaaca tctcaaaaag agagattggc gttcgtttta
    35161 acggtcgaac agttccgaat ttgcctactt atcgacaaat tattctccaa ggccattttc
    35221 agcttttctt acttgactct tctcaccagc cagagcctct ctgcaaagtt tccaaactta
    35281 ctttcctttt caacgaaatc gatattttgt tgttgggatt aatctacatg aggaaaactg
    35341 aaaagtcaag tacgaaattc aaattatttt ttctatattt atttttctgt ttttttattt
    35401 aacatttcaa taacagttcg aagatctttt ccttccgcaa gaagtttcaa tttttccttt
    35461 tcttcgacgg tataatttcg gacaaaactg gaatcaggta tcaaaacaac gcctaatcct
    35521 gctcgtgaag cagctgttgc tcctactaaa gaatcttcca ctgcaaaggc tgaactagct
    35581 tctaatcctg ttcgttccaa agctgtcaga taaggaatcg gtgaaggctt gttctccgac
    35641 acttcgtctc cgaatgtaaa cgtgtccaag taatcagcta atttaaagtg atccaagata
    35701 tctgttgctc ttttcgtaac agtggtcgta gcaatgccta gtttcatatg acgttctttt
    35761 aagtctgcca atatttcttt tgcatatggt tttaagtgga tggctccgct tgtcagctgt
    35821 tgataaaaat attcttcacg cttcgctcgg acttcctgta ctttttcagg gctacctgct
    35881 gctttcacta aaatatcaaa actttgtttc cagttttttc cgccccaagc gtcaacgatt
    35941 tcttttggga tatggatatc aaattgctga aatccccaaa gccagccatc acgatacgtt
    36001 ttttctgtgt caactaacaa accatctaaa tcaaatattg cacctttcat ctatacatta
    36061 atcctttcca aaacaataca catagtttag cacgactggt ccaaaaagaa cagaagcaaa
    36121 gaaccagcct tctcgtttat aagagttctt ggaatcgacg gatatagatt ttcttcacag
    36181 ctgtcacaag cgccagataa gcgatgactg tagcaaataa ccataaccag taattgccag
    36241 gaagtggcat caagcctaat tcagcaccaa accttgtaaa aggaaggaca gaaccgacaa
    36301 taattcctaa tgtagtgata acaaacatgg caaatgttgc gttgctttgg ataaatggta
    36361 ctttcggtgt acgcagtgca tggagcacta aggtttgtgt ccatagtgat tcgacaaacc
    36421 atcctgcatg gaataatgcg atgaaaatca ctcgctgtga actatccaat gtatggaaac
    36481 ttccgcccac tacagcagga caaataacaa aatacatcaa caagtaagta gtaatatcaa
    36541 atatcgaact tgttggacca aagtaaacca tgaattcccc aatactagat gcattccatt
    36601 tttttggctc gtccaaatat tctttatcca tccgatccca tggcaaggaa atgcaggaaa
    36661 catcatagat cagattcaaa aacaaaagtt gtaaaggcag cattggtaaa aatggcagga
    36721 acgtacttgc aatcagcacg gaaaacatat tgccaaaatt agaacttgct gtagccttga
    36781 tatatttcat gatattccca aaagtttctc gtccagaaag aaggcctctt tccaaaatcg
    36841 tcaaatcttt ttctaaaagg atcacgtctg cagattcttt cgcaatatcc acagctgtat
    36901 ctacagaaat accgatatca gccactttca ttgcaggagc gtcattgata ccatctccta
    36961 aaaatccgac agtatgacca tttttacgta aagctgtcgt caaacgtgct ttttgctgag
    37021 gattcaattt tacgaatact tcatggtctt cagctacttg tgctaattca tgatcactca
    37081 tttccgagat tttctcaccg gtaaccaatt cattgatctc tagacctact tgacggcaca
    37141 ctgaacgtgt aactaattcg ttgtccccag tcagaacttt tacagctaca ccatgttctt
    37201 tcagagcttt caacgcttgt ttcgttgttt cttttggtgg atcaaggaaa gcaagataac
    37261 caatcaaaac catgtctgac tcatctttga ctgaaaattc tccaacgacg cttggattgg
    37321 tcttttgtgc gacaccaatc actcgtaagc catcttcatt aagtgactgt acttttgcaa
    37381 gtacggcttc ttttcgttct tttgttaatg gtgtaacagt accgccaaga tcaataaatt
    37441 tagaaatgtt cagcatttct tcaatcgcgc cttttgtgat catttgtgtt ttaccagctg
    37501 gatcagcaac cacaacactc atacgacgac gttcaaaatc aaaaggaatt tcatccactt
    37561 tttgataatt gatcttatct gtatctaaag tttctgttgc ttcatcaatg atagccacat
    37621 ctaacaaatt tttcagccct gtttgatagt agctatttag atacgcatgg cgcaaaacac
    37681 gatcatcttc atttccttca caatccatat gatattcaag aatgatttta tcttgtgtca
    37741 atgtccccgt tttatccgta cataacacat cgattgcacc aaagttctgg atggcattca
    37801 aatttttgat cactgttcct tttttggcca tcacacttgc tcctttgacc aaattcgtcg
    37861 taacgatcat tggaagcatt tcaggtgtta gaccaactgc tacagatagt ccaaaaagaa
    37921 aagcttctag ccaatcccct tttgtaagcc cattgatgac gataactgct ggagccatca
    37981 atgacataaa tttgatcagt agcatggatg ttttatggat gcctaattca aagcttgatt
    38041 gaattggttt ttcagatagc gtttctgcta catgaccaaa tagtgtttca tttccaacag
    38101 aaacaatgat tccttccgca cttccactga tgacattact tcccataaag gcaagatttt
    38161 cgtaacttgt ttcgctttgc tgatcgtctt cacatacttt cgcatgcttt tctactggat
    38221 agctttcccc tgtcaatgct gcttgggaaa tgaataaatc ctttgcggac aataaacgca
    38281 tatccgctgg tatcatatcc cctgctgaaa gccgaacaag atctccacag acgatttctt
    38341 ccataggaat ctctgtgtac tttccattac gttttacagc tgccgttact ttcacaagtg
    38401 atttcagttt ttcagccgct tgattggaac gtacggactg aatcaacgtc attgtcccgc
    38461 tgacgataac catagtgaag ataatggcta caccgaaaag atctttatct ccaggagctg
    38521 ctaatacata ttccgtgatg aatgaaataa ttcctaatgc gatcaacact aatgtaaatg
    38581 gcgtgatgta tgctttcaat atttctacaa tgaatggtgt ttttttgcca tatgaaatcg
    38641 cattttcacc gtaattttct cttgaacgtt cgatttgttc ttctgtcaat ccatttgctg
    38701 atgcatggaa tagttcaaat aattcttgtt tcgtttttcc agcaaaagct gcatagattt
    38761 tttgtgtatc tttctttttt tccatctcaa tctctccttt gtgtccaaaa gacagctgtt
    38821 ctcgctgctt ttggtcttta gctattcatc ggatcgatag agaaaaaact gaaaggggct
    38881 gagaacagat agataatagc cctggccttt cctctagatc cttgtttttc gaatgactgt
    38941 catccatttg ttctcatctc ctttactaag taattttaat tcatgacttc caatcatttt
    39001 tcgaatagtt atttttccaa ttagaccacc tcctttttat ttttcaccga tggggatttg
    39061 cggcaacaaa aaaacctctc gccaaatact cagcgaaagg tttgaaaaag catattaaaa
    39121 aaagcttctc agatctcatc gttgagtttt gacactgtac aacgtaaaaa gcagatacct
    39181 gcttttagct accttatgaa aagccttatc tcgacaattc ctgttctacc cattggcgtc
    39241 tctcgacgtt tttgggcagt agcctatgtt tgtataggag cctcacctaa caaatcttat
    39301 tcggttttcc ttcgttaata taacaactaa aaaagaggat gtcaaatcaa ttttccatgt
    39361 ttttcatact ttttttaaaa tacacacgtt atattcctgt ctcacgcggt tttatttgtc
    39421 tcattttcca cctcccgcta taatagaaat atagtaggaa acgaggtgct gttatggaaa
    39481 gaacacttgt tattatcaag ccagacggcg tgagacgcca tttagtcgga agcatcatcc
    39541 aacgttttga agcaaaggga ctggccattg ctgaaatgaa gtttgaaact atgactcctg
    39601 aattagcaaa agaacattat cagcacttga ctgagcgcag tttttttgat gaactgatcg
    39661 attacatgac ttccggtcct gtcgtttatc ttgtgctggt cggcgaggaa gtgatcgata
    39721 tcgtccgtaa gatggttgga gcaacaaaag ctgccgatgc tgttccaggt acgatccgag
    39781 gagactacgc gctgccgggg actgaaaaca tcattcacgc ttctgattcc cgtgatgctg
    39841 cggtaaaaga gatcgcccgc tttttcccgc cattcgatat aaacaaaccg tctgaaaaag
    39901 cggcgaagtg atacaaattc taaactgctg gtctagttgc tgggttcagc aatggccagc
    39961 agttttttct ctaaggacct ccgttgattc tttcctaaca tatattcgag cactgtgaag
    40021 aattcttggt agtctttcgt cttttcttcc cctaaagcag cagaaatcaa tctttgataa
    40081 taatctgcca tcaatgtttc atagatcact gtacttcttt ctttatgtgc taagctccgt
    40141 ttcatatctt cattcacact acgtgccttt ttcaaccgac cattttctat ataaaacgaa
    40201 gtcaggttca ttaaaaaagt aaagagatac tgctcatgat tgttcaactg tgctaatcgt
    40261 cgcattcttt ttttgatttt caaataattg ttctcaatga accgatcact gaatatccaa
    40321 ggaaaatcag ctagaataga tagttcaaat tgaccatagt ctggtatctg catcaagtat
    40381 tcttcaatga ttcggcattc ataacgtaaa aattcaaatt ctgtctcaga tacttctgtt
    40441 ttttcgacca tgtgaagatt caaaaccaaa taatacagat cttctgtttt cttaaaatgc
    40501 ctacggttat tgctgatctc tttataagga tcaaagctgt cttcttgtac ccgatctagc
    40561 attcgtcttt ttttctttaa ctttattggt tcatccagct ggtttcgcat ccattcatat
    40621 tctgcaagtg tcatgttcat tcgctctaaa aatgatctca atgttacaaa tgacattttt
    40681 ttcactgaat ttgtctcaat cccgactaga gaagtcttaa aacagattcc ttccgctaat
    40741 tgggactgtg tcaacttctt gttgactcga agtttcttga tcaattcacc atcatacata
    40801 atagttctcc tttttaattt aaatttttcc acattcaata aatagatacg ttaaaaagga
    40861 aaaatacacg aaaaaaataa aaaaatgttt ttttcataaa aaataccaac cttcaagcat
    40921 tagaatgttg tctaactact gaaggttggt ttgattgttt attgattatt tttttgctgt
    40981 tcaacgtctg ttcttgttac tggatataag ttggttgttg ttttattacc attttttgaa
    41041 aagacacttg tcgaatgttc gcctttttgc ttttcgattg cttcattacg ttccgcttca
    41101 tttttatagt catatttttt cggatcaatc gtctcaaatc cgtttggcac ataaaaccgc
    41161 aagagatttt cctgattcag cttatctgaa agacttaacg cagtcttgac tttttcctga
    41221 tctttggaga tttcctcatc gattcctgct gctttcgtat cgatgatttc tcctgtctga
    41281 ttattgtaag cttttcctcc taggacggtg tatttgggtg tcacaaagtt cccatttcga
    41341 aaagcaacta cttgatcatg ctgagaagat aataagtctg taccaaaatg aagataatct
    41401 ttatcatcga ttcccaatag atgtagtaat gtcggcaaaa catcgatttc tccaccgtat
    41461 tcatgattga ctgtcccttt agtataacca ggcatatgga tcatcaatgg cactcgttgc
    41521 atctgtgcgt tgtcaaattc atcccatgta tctggatctt taccaagagc gcttgctaaa
    41581 tctttattat ctgtattcga gatcccaaaa tgatccccgt aaatcacaaa gattgaattt
    41641 tggtaaatac cagatttttt caaatacgta aaaaattgtt ccaatgattg atccagataa
    41701 tgtgccgttc gaacataatt atctactgtg ctattgccag tattcaaaga tggaaagtcg
    41761 aaattcttgt catccgtata ataaggcgtg tggttggtta cggaaagaaa tttcgtgtaa
    41821 aacggctgtt gtagatgctc aagatattgg gcagattctc tgaataaatc tttatctaat
    41881 atcccataac ctaacgtttc gtcggattca tcaaaataag atcgatcaaa gaaattgtca
    41941 taacctaagt ttttatatac atgatctcta ttccaaaaac tagctacatt cccatggaaa
    42001 acagcgcttg tatagccttg cttttgtcca aggatcgctg gtgcggcttg aaagacattg
    42061 tctgacccaa gttctgtgaa taatgagcct tgaggtaaac caaatgtgcc agtttccaac
    42121 atattttcag catcacttgt ctttccctgt cctacttcat gaaagaaatt gtcaaagctg
    42181 atcgttgctt gattctggaa aatactattt aaaaacggtg tgacttcttg tccgtctacc
    42241 ttcatattaa tcaaaaactg ctggaaactt tctaaatgga gaacgatcac atttttccct
    42301 ttagcgatcc caaatgtttc gggatttggc tctgcataat gtttctttgt ataatctaaa
    42361 acttcatcaa tcccattact gcttgcatgc gcgcgaacac tgctagtcat tcctgttttg
    42421 attccgtcat aaatagtata cgcatcgatt cctagatatt tgacaatata tgaacggtca
    42481 aatgttcgct gcagcaattg tggacggttg gcttcgctca gcgctagatt tcctgaaaaa
    42541 ataagccctg aaagacaggt aacagcgata gccatcggtt tgccgacagg gttgcttttg
    42601 ataggtattt tcttgacctt caaccaaacc agtagcccaa tgaacacagc gatatccagc
    42661 cagtagatga catcatgggg cttcatcaaa gaaaaggagc ttcctgacaa tccttgcgac
    42721 actttcgaaa agcctaatac agatttgact gtaatgaaat ctgtgaattc ccgataaaaa
    42781 ataatattca agtacaacaa aagcgtattt aaaatatcaa tgatcaaaag aatagctaac
    42841 gctggctttg gctttttaat atacaaagaa aggccaaaaa acaataaggt agtcgcaatt
    42901 gggttgatcc acaagataaa gtactggatc gtaccttcca cacctaatga aaaatctgca
    42961 tagtaggcaa tgatcgtctt cagccagaaa aagaaaacag ctgtcaaaac gattgctaaa
    43021 cgattttggc acaatttttt aagttgtgtc acgtatatca cttctctcta ttcacatttt
    43081 ttcttatttt gctcctgaaa aatgaaaatt tcctaaaaaa gttgttatct taagattttt
    43141 ttattctttc taaagattat tccgtccact tttcacagca taaaaccgct gcgatgtaag
    43201 cgattaaact aatgaagaga taaaagtctt gtggtgaccg cttcactcca cggtattcaa
    43261 aagccagttt ctcggtattc gtacaaaaaa agaggctgtg acaaaattct tgtcgcagct
    43321 ccttatttct tgatgcagga cagctttttc acaacttctt ccttaaacgg tgttctcctg
    43381 ccaactcctc ggccacaagt cacaatattt ataaaacatg agaagctgtt ttttgaaatt
    43441 g
//
LOCUS       DACDYI010000017        42967 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.017, whole genome shotgun sequence.
ACCESSION   DACDYI010000017 DACDYI010000000
VERSION     DACDYI010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.017"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(658..1014)
                     /locus_tag="IWM52_000883"
     CDS             complement(658..1014)
                     /locus_tag="IWM52_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAP9973155.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(1028..1438)
                     /locus_tag="IWM52_000884"
     CDS             complement(1028..1438)
                     /locus_tag="IWM52_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP9973156.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            complement(1785..3971)
                     /locus_tag="IWM52_000885"
     CDS             complement(1785..3971)
                     /locus_tag="IWM52_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP9973157.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            4189..4872
                     /locus_tag="IWM52_000886"
     CDS             4189..4872
                     /locus_tag="IWM52_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAP9973158.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(4953..5672)
                     /locus_tag="IWM52_000887"
     CDS             complement(4953..5672)
                     /locus_tag="IWM52_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP9973159.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(5984..6190)
                     /locus_tag="IWM52_000888"
     CDS             complement(5984..6190)
                     /locus_tag="IWM52_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973160.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            6435..7037
                     /locus_tag="IWM52_000889"
     CDS             6435..7037
                     /locus_tag="IWM52_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAP9973161.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            7178..8458
                     /locus_tag="IWM52_000890"
     CDS             7178..8458
                     /locus_tag="IWM52_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9973162.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            8471..9199
                     /locus_tag="IWM52_000891"
     CDS             8471..9199
                     /locus_tag="IWM52_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9973163.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(9268..10791)
                     /locus_tag="IWM52_000892"
     CDS             complement(9268..10791)
                     /locus_tag="IWM52_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP9973164.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            11043..12224
                     /locus_tag="IWM52_000893"
     CDS             11043..12224
                     /locus_tag="IWM52_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9973165.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKCLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(12284..13912)
                     /gene="treC"
                     /locus_tag="IWM52_000894"
     CDS             complement(12284..13912)
                     /gene="treC"
                     /locus_tag="IWM52_000894"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAP9973166.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(14309..17020)
                     /locus_tag="IWM52_000895"
     CDS             complement(14309..17020)
                     /locus_tag="IWM52_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP9973167.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(17030..17731)
                     /locus_tag="IWM52_000896"
     CDS             complement(17030..17731)
                     /locus_tag="IWM52_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9973168.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            17917..18516
                     /locus_tag="IWM52_000897"
     CDS             17917..18516
                     /locus_tag="IWM52_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9973169.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            18597..19610
                     /locus_tag="IWM52_000898"
     CDS             18597..19610
                     /locus_tag="IWM52_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9973170.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            19718..20635
                     /gene="rbsK"
                     /locus_tag="IWM52_000899"
     CDS             19718..20635
                     /gene="rbsK"
                     /locus_tag="IWM52_000899"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP9973171.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            20663..21058
                     /gene="rbsD"
                     /locus_tag="IWM52_000900"
     CDS             20663..21058
                     /gene="rbsD"
                     /locus_tag="IWM52_000900"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAP9973172.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            21058..22431
                     /locus_tag="IWM52_000901"
     CDS             21058..22431
                     /locus_tag="IWM52_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9973173.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            22788..24905
                     /locus_tag="IWM52_000902"
     CDS             22788..24905
                     /locus_tag="IWM52_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9973174.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIPETTTRTVKA
                     ATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(24957..25997)
                     /locus_tag="IWM52_000903"
     CDS             complement(24957..25997)
                     /locus_tag="IWM52_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP9973175.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(26274..28076)
                     /locus_tag="IWM52_000904"
     CDS             complement(26274..28076)
                     /locus_tag="IWM52_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9973176.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(28090..28842)
                     /locus_tag="IWM52_000905"
     CDS             complement(28090..28842)
                     /locus_tag="IWM52_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9973177.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(28851..29213)
                     /locus_tag="IWM52_000906"
     CDS             complement(28851..29213)
                     /locus_tag="IWM52_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9973178.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(29389..30261)
                     /locus_tag="IWM52_000907"
     CDS             complement(29389..30261)
                     /locus_tag="IWM52_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAP9973179.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(30312..30800)
                     /locus_tag="IWM52_000908"
     CDS             complement(30312..30800)
                     /locus_tag="IWM52_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP9973180.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(30938..31768)
                     /locus_tag="IWM52_000909"
     CDS             complement(30938..31768)
                     /locus_tag="IWM52_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAP9973181.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            complement(31784..32392)
                     /locus_tag="IWM52_000910"
     CDS             complement(31784..32392)
                     /locus_tag="IWM52_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAP9973182.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(32385..32702)
                     /locus_tag="IWM52_000911"
     CDS             complement(32385..32702)
                     /locus_tag="IWM52_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9973183.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(32858..33586)
                     /locus_tag="IWM52_000912"
     CDS             complement(32858..33586)
                     /locus_tag="IWM52_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAP9973184.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            33745..34626
                     /locus_tag="IWM52_000913"
     CDS             33745..34626
                     /locus_tag="IWM52_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9973185.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(34863..35906)
                     /locus_tag="IWM52_000914"
     CDS             complement(34863..35906)
                     /locus_tag="IWM52_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9973186.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(35909..36577)
                     /locus_tag="IWM52_000915"
     CDS             complement(35909..36577)
                     /locus_tag="IWM52_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9973187.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            36719..39055
                     /locus_tag="IWM52_000916"
     CDS             36719..39055
                     /locus_tag="IWM52_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9973188.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGNI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(39170..39481)
                     /locus_tag="IWM52_000917"
     CDS             complement(39170..39481)
                     /locus_tag="IWM52_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973189.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            39662..41137
                     /locus_tag="IWM52_000918"
     CDS             39662..41137
                     /locus_tag="IWM52_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAP9973190.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            41184..41768
                     /locus_tag="IWM52_000919"
     CDS             41184..41768
                     /locus_tag="IWM52_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAP9973191.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            41770..42189
                     /locus_tag="IWM52_000920"
     CDS             41770..42189
                     /locus_tag="IWM52_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAP9973192.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(42470..42754)
                     /locus_tag="IWM52_000921"
     CDS             complement(42470..42754)
                     /locus_tag="IWM52_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAP9973193.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
BASE COUNT        12802 a         8585 c         7556 g        14024 t
ORIGIN      
        1 cgattgttaa gctcaatttg tttgctagca tattgcttgc ttgttaaaaa atgtttgttg
       61 tcttgaattg aatcaagacg ccctacacat ttggtttgtc ttactttgtt cagttttcaa
      121 aggtctactt catcaggagt gttccccgat tcgtttttgg ctgccgtagc aacttttata
      181 tcatatcaga gagacaagta attgtcaact cttttttaaa agtttttttg atttcgtttt
      241 tcgaaatctt acagcaactc atttatcata acttatcatt tgttatttgt caagaacttt
      301 ttttgaagtt tttttcgata gaatcaagct tcacaagtgg ttctttttta acaactcagt
      361 tatcttatca tgtcatttct aacatgtcaa caactatttt atatgttttt taaaatacta
      421 ttttattgct ttgctactta aaacaacaga aatgaatata tcatgtaaat ttacacatgt
      481 caatatattt ttaactctat atccccacct agatttgaat tttgaccaca taaattgagc
      541 atgtctctat agtagaaaaa taaagtcaaa aaaagaacac ataacacgct tgatgctaag
      601 tgttcgactg ttacaactgt atgaaatgct cagtgacttg attaaaacag ttaaagtcta
      661 tttatttaag ataagctgtt ccatcaactg ctcattgtat ccaataaata gaacttcatc
      721 gataataatc aaaggacgtc tgaggagatt ggtatgttgt gtgcataaaa aaatcagttg
      781 tttttttcct acacaggaaa ggtcaatcgc aatgttgtga aaagcctcag accaagtcgc
      841 aaatagctgt tctgcttgca cttcttcaaa actgcataat tcttctgctt cttctgtact
      901 tagatcgcgt tcttctaagt caacaaattg ataaggcaat tgatggtgtt ctaaccattg
      961 tttcgccttc agacttgtta cacagtgcgc tgatccatag actttcactt ccacgaaggc
     1021 tccctcccta tgaatttaag atgttattgt tttgtaaata gatcagattt ctggcaacac
     1081 atcgaatttc acgagggaca aatgaccgga tctcatcttc attaaatccg attagtattc
     1141 ttttttcatc aatcaaaata ggtcgtctca ataatgaagg atattctttg aataagacga
     1201 tcatttgctt taaagttaat tcgttaaaat ctatgcccag cttttcgtaa attttcgacc
     1261 gagtggaaat aatatcttga actcctcctt cagtcgctgc caagatcttt ttgatctcac
     1321 tttctgttaa cggttcggca aaaatattct tctcttcaaa ttcaagttga ttcgcttgca
     1381 accatgctct tgcttttcta caggaagtac agctaggcga agtatatact tttatcattt
     1441 tctcactcct ttttaattat aatccaattc tacagtaatt tcttttaaaa taccaattat
     1501 tagctaataa aatataaaaa aaaatcaaaa taactgatta tttacgtttg aataatgaga
     1561 atttttctat atattctcta ttttgataat agtattctca ttttcaaaat aaataaggga
     1621 ggtgtgacaa aattcttgtc acacctccct tttccgaata aactgtgttc cagaaatagc
     1681 ttcttcgaaa ataagctgaa atttcacaaa aatttgagaa gcaattttcg tacattcctt
     1741 cttctttctt gaaactgacc acttctgtca caccctctct tctatcactt tccttttttc
     1801 acacgactcc cggtttcttt gataaatctc gatactttca attttcttga atttttcatt
     1861 tgaggtacac atagatacag acgatttctt gctctagtca tcgcaacata aaatagtctt
     1921 cgttcttctt ctatcgtcgt cactggttct gcttttattt cattaaagtc gattactttc
     1981 ttttctgtcg ctccgttata aggcaaactt ccttcgttac aaccaatcaa aaagacattt
     2041 cggtaactaa gacctttact tttgtgaatg ctctggatac atagtgcatc aagttttcct
     2101 tgtctgtaag caatcaagcg ggtttttaat tctttagctg tcgcttgggt ttcttgcgat
     2161 aaggcttgga gtcttctgaa agtagcatgc tgctttactt cttctcgtat ccattcttga
     2221 tcactctggt cgatttcctt agctatacgt ggcagatgaa gatagctttg aacaagcgaa
     2281 tagatttctt gatgctggtc tgttttacgc aacgcttcct caaactctcg gatttctgca
     2341 gatttcgatt ttaaattggg gcttatcgac aaaatataat ctgcaacggc tatattttga
     2401 tatttaggag aaaagtattg atcgcattca ctttttttca ggtagcagtc tgccatcagc
     2461 tgccggagca attgctggta ggcctgattt ttcttttgca gattcagtgc tgtttgccaa
     2521 tgaacaattt tttgatagaa atgttggaaa gacttgatac caaagatacc ttcatatgga
     2581 ctatctgctt ccagtacaaa aggaatattc gttttcagca gagattggac aagctgctgc
     2641 gtttgtgaag ctgatcggta taatatgatt gattccttat atttcccatt tttttgaata
     2701 aatgtctgaa tttgtttgat tagccactga ctttctgctt tagtatcaga aaacaaatag
     2761 gcttccataa cacctgcttc tttttgtgct ggaatcatcg cctttggtat ccgatcattt
     2821 ttattatagc tgatcaatct atttgctcct tggacaatag ctgcccgaga acgataatta
     2881 gttaataaga aaagagtttt ggtaggatat tctttttcaa agttcttgat gtatgctggc
     2941 tctgatcctc tgaatccata gatactctga tcatcatctc caacaacaat caacttgatc
     3001 ctgttttcct ttgtcatccg tttcacgatt tcccattgaa gtggattagt atcttgaaat
     3061 tcatcaatga atagataatc atatttttgg ccgatataat gctggatctc ctcattctcc
     3121 aaagcgcttt tcagattttg gagtacatca ttgaactcta gcaattgctg gcttcttttt
     3181 tgttcttgga gttccgtgag caatccatac agtcgttctg atatcggctg tcgtgcattc
     3241 ttatcaatgg aatcatattt ttctttaact aactgaacca tttcttctac tgaaaggttc
     3301 atattctgcc aatagtctaa ccgagaagca atcgtttttt ctccatattg atcaaatata
     3361 taaagattat atttacgtat cagctgttgc aaaacagcat tcactttttg tgcagtatcc
     3421 tttgaaaagt ctccttgtat tccttgcatt gctacttcag gaaattttcg acaaatttca
     3481 ttgtatatcc taaaaaagat actatggaat gttccgatat ggacagcggt ttttctctgg
     3541 ctgaaattat ctactcgttt ttttagttca tttgcagcgt tccttgtaaa agtgatcatt
     3601 agtatgcgtt cttcgtctac ttgtttttgc gtcatcagat aaacagcttt tgcacagatt
     3661 gtttgcgttt tgccgctccc agctcctgct atgattcgaa gatgtttctt catatcatat
     3721 gtgatagctg ctttttgttc ttgattgaag cgattatagg aacgtttctc catcatttta
     3781 aagaaagagc tgtttccttc actttctttt acttgtactt cttcctcaat aagtccgggt
     3841 gcttcgccat aagccgcttc ataggtagca atgatttctt cttgtagccg aaagacttgt
     3901 tgaagctttt tatacttgtt ctcccagcgt ttcaattctt tttgtaatcg gagaatttct
     3961 ttttcattca actgattgat ttccttccca tctttcttct ttgaatttgt ttatttcaag
     4021 ttggttattt aaagtttcgt tgtctatttt ttctttatac taacagacct ttctaaaaat
     4081 gaaaatggga agcgatatga ttttagtttt ctgaaaattt ttcctttttt tcaatcgttt
     4141 atgctaagat aaggaagaca aaaatattca taaaaggtga tgaaaaaaat gcagacttcc
     4201 tttcacgagg ggcgtacaat aggacttttg ctgacattta tcggaggagc catggacagt
     4261 tatacttata tccaatatgg tgcttttgcc tctgctcaaa ctggcaatat cattctatcg
     4321 atcatccaag catttgacgg agaatggcta agtgttggta aaaaagtatt gtctaccctt
     4381 ttctttttct tagggattct tctaacaaag tatctaattg attattttag aaaaaaagaa
     4441 aaacatttct ggcgcttgtt tgttctatat tatgaagcag tgattttttt ctttgttagt
     4501 ttgacacccg tccatgagca tcccgcatta gtaacgattt tgattgcttt tactgcatcg
     4561 atccagtggg tagcttttga taaaatcaac gggcgtgcct acactaattt atttacaact
     4621 ggaaatttaa aaggggtagc aacgaactta tatgattatt gtatttctaa agaaaaagca
     4681 gattttgatc gatttttcca ctatctaagt gtcgttcttg cctttatcgc aggagctatt
     4741 gtccttgttt tctgtcatca tctgtttggc gcccattcga ttttgatcgt ttccatttta
     4801 tttttgatca tggccctcac tcagacattt catttatggc aattttatca tacgaatcgg
     4861 ttcactattt gatctcaatg aatcaaaaaa actaagcaag atgctgctca cagagaaata
     4921 tctgcgagtg agcaatttgc ttagttttta tattatttag ttgtaaagat tccctttgct
     4981 ttcgggttat ttgtagtgta gcggatcgac tgggcatctg cctcttctgt cattttcttt
     5041 gcccgcatct tgatttcttg tacgatttgt tctccacatg ccggatcggc tacgaaatca
     5101 tatttgtccc catcgatcac tattttggga catatatcaa actgttcata ccactcttcg
     5161 tatcgtcgat tgagttcttt atagtaggaa tagagttcag gatcttgttc tagctgttca
     5221 tattcccggc ctcttttttt gattctcgct aacatagttt caaaggatac acgaatataa
     5281 accagtagat ctggtttctt ttgtggcgaa atttcttcta actccttcag catcgtttct
     5341 aaaagacctt catactgctt tacttctact tcagaaacac gccctaaatc tgcattcaaa
     5401 tgaaacagca tactatcttc gtagatggag cggtctagta cgttgtcatc atggctgaaa
     5461 gcatctttga ttgccaaaaa gcgtttgttc aaaaagaaaa tctgtaataa aaaagtatac
     5521 ttttctggat tagaataaaa tagcggcaat acttcattat cttccacgtt ctcataaaaa
     5581 ggttttgttt ctaaatcttg ggcaagcatt tcagttagtg tgcttttccc tgcaccgatc
     5641 gttcctgcaa caatgatcac aattatcggc atccttcctg taataaaatc tgttcttcat
     5701 tttactactt catttataga aataaaagcc ttgactttat ctgctcctta aaagctactt
     5761 ctctaaaagt agccaaaagt aactcctatt catgaaaata aaggctgtga caaaagtctt
     5821 gtcgcagccc ttttttcaaa taaacagtat tctcctgcca acacctcggt cacaaggcaa
     5881 tattcataaa acatgaaaag ctgtttttta acattattcc ttgtaactca gagctaaacg
     5941 ggagactcac aacctcttta tgctctcaac attttatctg tttttattct tctattgtac
     6001 ttgatttgcg tacaactaac tgccagtgca gattatgagc ggtatcattg tcttctactg
     6061 cgcatttcat gacacgtatc atcacactat tttcatagat tttttctatt tttcctttta
     6121 ttggtgattc aaataaatca gatttaaatg catatgtttc gccgacttca aacaattgtt
     6181 cattttccat aagaatccct ccatcacctt ttataaggtc agtaattttt taacataaat
     6241 cactcgaaaa agcacaaact tgtcataaaa agtacttttc gtatcaaaac tgtcactttt
     6301 tttggtaagg ttcttggaaa acgaagattt ttttaggaga tcatgacggt tttgatgtca
     6361 aaaccacttt ttatgaaaca aaagacgttt tttatttttt ttagcataaa atttgtggtg
     6421 gaggtacgta tgagatgaaa gataaagttg tgaacaaatt attagaagcc ttattcagcg
     6481 gagacaaatc ggaagaagat atggtgtatg tccaagtaaa atttgcctta gaagttttat
     6541 tgaataatct tggaaaacta gcggtcgttc tagtcttttc tctggtgaca ggctcatggg
     6601 cggaaacagg aatcaccttt ctttcttata tctgcatccg cagatatgct tatggattgc
     6661 attccgactc tgaattcgtt tgtctgctgt ggacactact ttatttatgg ggcgttccct
     6721 tggtgatgaa acatctgcaa ttgactatct ccttcccaat gatggtcttt ttgttaatca
     6781 gctgcttttt gttactgctt cgctatggat caagaggtac agcaatcaat cccattgaac
     6841 ccgagaaacg tcccccatta ttaaagaagg cgatcagcat gtttctaatt ttttctctca
     6901 ttactttatt tttctcagct tcttactttt ctacctattt gctacttggg attgttttag
     6961 aaatagctac gttgcttcct attacaaact acttgatgaa ttttggagga atctttcatg
     7021 aaaaaaacaa tcattaaaca attaactaac ttatctgact tttttgtatc tggctgctgc
     7081 tttggtatga gttctgaatt caaaatccct gaaaaattaa aaaaataata cgctaattgt
     7141 gctataattt ggtaaaaatc aatgggagct gtactttatg tttttatggt ccatagcgtt
     7201 gcaatatttg atttacacaa tttgtatctt attgattgac catcacatcc gatacaaata
     7261 tctgtttgcg ggagtcattc tgatttcttg tattttgatt ggatgggtct attcgatttt
     7321 agggattttt actgctctaa ttgctatagg aatgctgatg cttacttttt tcttcttttc
     7381 gacttcgcgt caacaattgg tttacgcgct tccttttggg ttattgacgt cgcttttagg
     7441 agatcactta acaagcatcg acgatttttt tgttttgaaa tcttcggtta ttgaaccagg
     7501 agattcattg cagtattttc acattgtctt atccgctttt ttatctgctt tattagcttt
     7561 ttgtttaaaa aaatcgttag agacttggtt tcacactgtg gaccgccgta tgatcggtac
     7621 gatcgggaca ttgatattat ttacatacta tatcattatt ttttataccc gtttttctgg
     7681 tgaaacacct gatgtcttag ctttgaatac gagttttttc attctatatc tgtgtgttgg
     7741 cctgatcatc ttggtttatt attggaaaat ctctaataag aaattagctg atcagttatt
     7801 agagcaacaa gtcaggatgc aacaggatta tatcaaagat ctagaaaaaa actatcaaga
     7861 cttgagagaa ttcaaacatg attaccaaaa tctgcttttt tctcttcaca gctatatttc
     7921 agaaggcgac ttagaaggat tacggtctta ttatgatcaa acgatattac ctacacgcca
     7981 gatgatgaac ctttttccgg ccaatctaag tatgttggat aatatgaaag ttcctgagat
     8041 ccgtagttta ctgtcattaa aactcatgat ggcacaggaa aaagggttga tcgtccagct
     8101 tcattttcct gagccaatcg aactggatac gaaacatacg gtaaaccttg tacgtatgct
     8161 tggtattatc ttggacaatg cgattgaagg agctagtgct gcaaagaaga aaaaaatcga
     8221 attgaccata ctcaagaaga atcattccgt gatgattcgt gtgatcaata caacaacaaa
     8281 tacattaccg ttaaaccagt tgaaacaaaa agggttctca acaaaaacaa atccaaaaaa
     8341 cgaaggcctt ggcttgttta tcttggatga attgacaaag accaatcctt atcttttcct
     8401 tgaaacatca attgaaaacc agcgtttttc tcaaacgatt tatatacaaa taaaataaag
     8461 gagtaatgat atgattccca tttatatttg tgaagacgat ccaaaacaat tagaagtttt
     8521 atcaacggtg atcaaaaata gaatcatgat tgaaaactta gacagttatg tccatatttc
     8581 tacctctgat ccggaagaac ttctcaatgc agccaagatc agaacttctt cttttggtat
     8641 ttacttttta gatatcgaac tagaagacag tgaaatggat ggtgtagcga tcggtaaatt
     8701 gatccgtgaa ttagatcctt taggaaaaat aatctatgtg acttcccata cggaaatgtc
     8761 gatgaaaatc ttaaaaagca acatcgaacc gactgattat atcgtcaaag aagatcttta
     8821 tgatttaaaa gaaaatgtcg agcatatcct gagcaaaatt ttcgaagatt ttcaaatgtc
     8881 ccaaatccaa aaagatatct tcaaaataga gtttaatgat gagatcaaat ttttaccgat
     8941 caaagacatc caatattttt ctacttcgct aggagcgcca cataaactgg aagtccattt
     9001 gactcatgcc caattacaat tctatgagaa gatcaaagaa atcgagaaaa tgcatcggaa
     9061 ttttatccgt tgtcataaat cctatgttat caatacacaa aatgtgcggt cgattaataa
     9121 aaaaaccaga gaagtcacgt tggcaaacgg cgagaccatc ccagcctcta tccgtggatt
     9181 aaagaaattg atttcataaa aagaaccgaa tagtttggac cgactaaatg accagccgat
     9241 ccaaaccctt cggttctttt ttgcctatca gtgcaaaagc atttcctgtg caatttcacg
     9301 tccatccatt gtatctggat aaaatgttgg ccaattctcc atttctttca gcaatacttc
     9361 ttgactttca tttcctgcat ataaatgaaa atgttcggag tcagccggtg caatcgtgtg
     9421 atcactgaat tgaatatatc taaaagcgcc actttcctta tcttctgctt cgaatagata
     9481 ccttacgcca cggtttcctg cctcgtaggt caagatttct ttccctacat aacgataatt
     9541 tgaacgtttc gttgttccat ccttaaacgt aaatgccatc tctttctcac tgatcttgat
     9601 tgaagcaata tctgtttgat agcccttcgt ataatattct ttatattctt gtgctgtctt
     9661 atcctgattc aatttttctt tatattcaaa aacaggatca aaagtcccat cttctacata
     9721 cgtatctact gtttgccaag ttcctgccca atctgaaaga gagcgatctt gtacatcctc
     9781 gtcttcaaaa taaccgttgt agacgctctt ttcttttttt ggggtcaaag tatcttctag
     9841 cgggttttct gtctctgtcg tttttttcaa cgcttcgagg tttgcttcca tgatagaaag
     9901 atagttttct ccattttcta tttgttcatt cgtcaatcct tccaaaggat tcaatacttc
     9961 taaagataca cctgcttcgt ttgctaacgt tctggcaata ctatctttgg cgttttcttc
    10021 aaaataaata tagttgattc catgttcttt aacaaagtgt ttcagttcag caattctcgc
    10081 cgcagacggt tcttcactag aagaaagacc agcaatcgat acttgattca atccatattc
    10141 taacgctaga tagttgaatg cagcatgttg cgtgacaaaa gatctttgtt ttgcttgctt
    10201 aaaggtttct tgataaagct gatggagtgc ttgaagtttc tcggtatagg ctgctgcatt
    10261 ttttgtccat acttcctgtt tttctgggta agcttcaatc agctgatcac gtatgtttgc
    10321 tacctgcttg attgctaaac ttggagctag ccatacatgt gggtctagct catgtgtatg
    10381 gccttcttcc ccgtgttcat ggtcatgatc atgtccttct tcacttcccg gaagtaaaag
    10441 cataccttct gtcgctttta tcgtatggac attgccttct tgtaatgttt tttggattgc
    10501 ggggacccat gtttccatgt tttcatcatt ataaacaaag atatctgtat cttggatctt
    10561 tgccatgtct ttagcagaag gttcataatc gtgggaatca gtccctgctg gaatcaataa
    10621 ctctacttct ccttcatccc cgacgattgc ttttgtaaaa tcatacatgg gataaaaggt
    10681 cgtcatcacg ctcaattttt ctttactgtc ctcggctttc ccacttgccc cgcaagcagt
    10741 taacatacct gctaaaacca gcatggccaa tatacctatc aatcgtttca tttatgacac
    10801 tcctcttctc atcttctttc atttgaatgt tccgattcgc ttttcgcaaa ccgtaatagt
    10861 tacgttttag caacaggatt taatttatca tatggttaaa agaactgtca agcgttttct
    10921 tattttaatt gatgagaaag tttatatgaa tgattgacga tatttttgca atcaacctct
    10981 atcattttcc ttttataccc gcttttgtct ataatgaagt tatagataaa ggaggaaaaa
    11041 gagtgaaaaa agaaacagat acaacgaatc gtttgatccg ctttttagga ggaaaaacct
    11101 cttactatgt tttaggaata gttatcttac ttgcgctgat catttatttt ttccatcaga
    11161 tctcgtttat attcaagcct gtcggaatca ttatcgctac tatcttgccc ccattattgt
    11221 ttgcactgat cttgtattat cttttggaac cagtcatcaa atgtttgagc aagcaccttt
    11281 cgaggaactt gtcagtcatt atcgtttatg tgattatttt atttttattg gggcttggaa
    11341 gcatatggct tattcctttt cttgaagaac aggcaaagac attgatcagt agtttgccag
    11401 atttgtttca agattttcaa gagagtctcc gacaattttt ggagaaaaca ccttttgccg
    11461 attcatttaa tcaatttttt gcttctattg acgacgtgac gtctgatata gctggattta
    11521 ttggaaatta ttgggaaagt ggcgcacaac ggatcagcaa cattttttca actgttacag
    11581 ctatttttat tactttattt actggtccaa ttgtcgcttt tttccttttg agagatcctc
    11641 aaaaatttta tcagtcagtt ttagcgattg tcccaccagc atttcgtaca gattttaaaa
    11701 atttgacaaa gattgctaat caacaattgg gttcatttct aaaggggcaa atcattgctt
    11761 catttatcct tggagcagtc tactgggttt gtttcttact gatcggatta gaatttgcta
    11821 gtgttttagc tatttctgct gggttgttat gtatcattcc atatattggg ccgtttatcg
    11881 ccttctttcc aggtttgatc attgcattcc aagactctac ttttatggct gtcaaatttg
    11941 tcattgtctg gtttgccgta caacttctcc acggtgactt ggtgattcct cgagtaatgg
    12001 gtgatcgttt acagatccat ccgattacga tcttaatcgt tttacttgtc atgggtgatc
    12061 taatgggaat tgtgggcgtt atttttggta ttcctatcta tacgttagtc aaattactcg
    12121 ttacttttat tttccgtaaa ttcaaacaac gctacaataa attttatggc gataaaggag
    12181 agtatgaaaa ctcttcattt tctgaagaag attattttaa ataaaacaca aaacgtcgat
    12241 cttacgagaa tagctcgtag gaacgacgtt ttgtgttttt gttttaattt ttcagcaaag
    12301 caattgcctg gtatggtttt agctccagac tttgatgaag aacagtatcg gaataattat
    12361 cgataagaat attggcttct gtgaatcctt ctgggatggc gatccttgta tctttatcat
    12421 agaaatttgt taatacaagc agctgctgtc cttcaaattg gcgaagaaag ccataaactt
    12481 ccggatgatt tgcttcataa gcatgataat ctccttgtgc aacgattgga tatgtttttc
    12541 tcagccgaat caatttcttg taataggaca aaatagaatc ttcagaagat agttcttttt
    12601 ctacattaat ctcttctagc ttgcctccaa cttttaacca aggtttccct gtagtaaatc
    12661 ctgcttgttc tgtgtctgac cattgcatgg gtgttcgaga gttatcgcga gattttgctt
    12721 tgatccgacg aaacgcttct ttttctgtga atccttgttc aaggagtaac ttataagcat
    12781 tcaaactttc tacgtcaata tagtcggaaa tcgatgaata gtctgggtcg atcatgccga
    12841 tttcttctcc catataaata tagggcgtcc cacggttcaa atggatcatg gttgctagca
    12901 tctctgctcc ttgttttcga tactgatcga tagctacaaa tcgattgagc gcacgtggct
    12961 gatcatgatt attaaaaaat agagcattcc agccgttacc ctcactcatc ttttctcccc
    13021 atgtatgaaa tagtcgcttt aattctgcaa aatcaaatgg aggagtagac catttattcc
    13081 cattttcata atcgactttc aaatgatgga aattaaagac cattgataat tcttctctac
    13141 taggttctgt atacaagata caattttcga tcgtcgtaga gctcatttca ccgactgtga
    13201 tgatagtgga atccgtccca aacgttgctt ggttcaattc ttttagatac gtatgggtga
    13261 ttggtttgtc cgtatattca ggtttgccat cattttcttt attatttttc aattgttcgt
    13321 ctttaccgat aacattgata acatcgaaac ggaatccttt tacccctttt tccatccaaa
    13381 aacgaacaac tttgaatagt tcctctctta ctgcttggtt ccgccagttc aagtcagctt
    13441 gtgtccgatc atataagtgc aaataatatt tccccgtatc tccaaatggc gaccaagcac
    13501 ttccaccaaa ttttgatacc caatccgttg gctcttctct taaaatgaag aagtcctgat
    13561 aatgctggtc ccctgctagt gcttttttga accattcatg ttctgtcgaa acgtgattca
    13621 gcaccatatc gagcatgatt tctatccggt atttctttgc ttcggctatt agctgttcga
    13681 attcctccat cgtaccaaag ataggatcga tggctgtgta atcagagata tcataaccgt
    13741 tgtctttttg agggcttgga taaatagggt ttagccaaat catgtcgatg cctagatcat
    13801 tcagatacgg gagtttttct ttgattcctt ttaaatctcc aattccatca ccattactgt
    13861 ctttgtaaga tttgggatag atttggtaaa tgactttgtc tttaaatgtc atggatattt
    13921 acctccattc aactgattta ttacctacca caacttatat atacaagtta tcatttatta
    13981 ttataactga accgaaagcg tttgtaaaat ttatctttat acattctaag aggtctgttg
    14041 cccaataaaa aacaagagat cagacatgta tttgtctatc ctcttgttta aaatagcaaa
    14101 atattcttgt ttcttctata aatggtgttc aaaagctttt atctacggca atcagtcttc
    14161 tcaaatgtaa aacatgagga gctaatttta tttgagtatt ctttttgctc gatgcaggac
    14221 ggctttttta caacctctga ttcatggtgt tcaattagca gctgaccctc gttattagct
    14281 caaaacgctg atttcacaac ctctgttttt attcattcga tttcaacgca tcaatcatat
    14341 cgatatgctt taatttgaaa tgggtaacaa tcatcacaat aaccgtaaat acgatagtta
    14401 atcctgctga taagacataa gcccaccatg tgatcactaa tggaaagatg acattctcca
    14461 tagacgcttg ttgcaagatg aagtcagtca atatatatcc tacaccaaat cctccgatga
    14521 ttcctaaaag agtaaagatg atattttctc gaacaatgta catcgtgact tctttatcaa
    14581 aaaatccaag gacttttatt gtagataatt cgcgtacacg ctcggaaata ttgatattag
    14641 ttagattata caagacgata aaagccagca gtcctgataa tacgacaaag attaagacaa
    14701 ccgagtctaa gttcgccatt gattcttctt gcgtttcgat ttgtttggac atgaaagacg
    14761 tatttgtcac agcgtctgtg gcaagcagtt tctcggaaag catttcttct tcttttgttg
    14821 tcatcttatc tgtacgcagc aaaaatgcat tatccatcat cgattgatca gtaactgaag
    14881 cataatattc aggggacata taaataaagc tacctagata gtttttcacg atttctttta
    14941 cttcgacttt ccattctttt tgatcttcat cataaagcga aatatgatct cctgtagata
    15001 agtcaaatag ttctgctact ttttgcgtaa tagcgattcc tgttttaggc aaggagcgtg
    15061 ggttctcttc aagatcagtt aaatggataa aacgatccaa atcagctgtt ttacttggta
    15121 cgatcactgt cagactttgc gaagcatgtc ctttttttct caattctacg gtttgagtat
    15181 gaatagctag gatctcattt actttttctt gcttctgcag catatccgct gctttttgac
    15241 gactatcttc cgtggatgaa tccaatgtaa cgattgcttg atagtcaatg atcggtccaa
    15301 attgtttggc tgaaacagaa cttagagaat ctttcaatcc cacgcctgca accatcagcc
    15361 cggcacatcc agcaattccg ataatcgcca taagcattct tgacttatat cggaagagat
    15421 ttcggtaact gattttttga ttaaagctaa ggcgtgacca cagcggagtg atgtattcaa
    15481 gaaaaatgcg tttccctggc ttcggtgctt tcggttgaag caatgcagca ggccgttctc
    15541 ttaaatcttt gaataaaacg atcattgcag cgccaaatgc ggcaatgaag aatgcggcag
    15601 ctgcttgtaa aatcggcggc cagtcataaa aaataagcgc actcccaata tcataccgct
    15661 cgttagacag ctcaaaaatg atccttggca aaagattcgt tcctaatatc gttcctaaaa
    15721 caatacctat ccctgaggct agacttgcat agattgcata tttttttgcg atttcaactt
    15781 tcgtataacc taacgccttt aatgtcccga tttccctgcg gttttcttct accatacgtg
    15841 tcattgtcgt aaaggtgatc aatgcggcaa taaagaaaaa gaaaacagga aaaacattgg
    15901 caatcgcagc gatacgatca gacagatcac caaattcttg aaaaccgaca ttttctgaac
    15961 gttcattgta taggtatgat ggtttagtta aattttcaat tttttctgca tttcgattca
    16021 attcttcctc tgcagaggcc agttcacgtt ctttctcaac taattgcctt tcttgttcag
    16081 caagttgagc ttcttgttga gataatgttt gaacagcgat tgttcgttgc gcttcaggaa
    16141 gctgctggat agttgctgac tgttgcgcta actgtttttt agcatcttct aaccgtgttc
    16201 ttgcttgcgt tgtttgtgct ttgccatccg ctattttttg ctgtgccggt tctaattctt
    16261 cgttggccga ttttttcagc tcttctaagc gttcttcggg tcgatcttgt aaatactttt
    16321 ctgccttttc ttgattttgc tccattttat ctttgtaagc tttgctgtac gtattcaaat
    16381 tctttacgtt tgtaaaatca agatataaga cagaataaat atctgtttta aaaacggatt
    16441 caggtaaata gacaaaataa tcgacgctac catttccgac gttcgcgtat cctctctcca
    16501 aattgttgat gaacaatgga gaacggacaa agcccgtgat ggtatactct ttttctttca
    16561 attcatctga atcaatctga taaatatcac cgattttata cccttcatct tccgcttttt
    16621 catccaatac aagctggttt ggtttttccg ggagttttcc atcagttaat tgtagcttgt
    16681 tctgcttgtt atttttgtta taagaaaata tttgaatcac ttcgtttttg ccagtattgg
    16741 aataatagat ctgttttcct gtttctactt ttgcatctgg gatcttttct gcttgagcaa
    16801 tatctttttg tgtcaagcca ccagtagaaa cgatttgcaa atcagaaagt cctgctgtac
    16861 caacatagtg atccatcgtt ttttgcatga caggtccagc agttttgaca cctacaaata
    16921 aaagtgttcc tagcatgaca ataaggatga ttgcaatgaa tcgtccttta gattgccgaa
    16981 tttcgcgaaa acctgcttta agatatgttc ttgttttcat aaaatccccc taccatacaa
    17041 tctcatcaat ggatgaagga tgatcattta attcaacaga ccttacttta gcatcattaa
    17101 tgtgaatgac tctatcagcg atgggcgcta aagcagaatt atgtgtaatg atcaaaacgg
    17161 tattgccgtg ttgtctgcta gcgttttgca ataatttcaa tacttgcttg cccgtttcaa
    17221 aatccagcgc gcctgtcggt tcatcacata aaagaagttt gggattttta gctagtgcac
    17281 gagcaattga aaccctctgc tgttcgcctc ctgataattg ggacggaaag ttattcaatc
    17341 gatgagctag gcccacttgc ctcaatacct ctacaggatc aagcgcatca ggagaaactt
    17401 ccgttgccaa ttccacattt tcttttgctg ttagattagg aacgagattg tagaattgga
    17461 aaacgaaccc tacatctgtt cgtcggtaag cagtaagctg tttatctgaa aattgcgcaa
    17521 tatctgtgtc atcaataatg atctgacctt catcaggtgt atccatccca cctaaaatat
    17581 taagaatcgt tgactttcct gctccacttg gaccaagtat aatcactaat tcgccttttt
    17641 caattgaaaa agaaatatcg tcatttgctg taatggtcgt ttctcccatt ttgtatcgtt
    17701 tgtattcatt ttttacttcg acatacttca ctctgattcc tccattttca cgatatttct
    17761 cgctatatct atgcatcgaa tgcgttttct cacacttatt tattttacca ctgaatttct
    17821 ctagactaaa gaaaggaatc ctttaattta gttgacaaat aaacgattta tgatttactt
    17881 acataaaaaa cattgttatt tgaaagaagg aacatcatga atgatttaaa ggaaaatatg
    17941 atttttgatt tatcaaaaac tgcttttgtc gcgatcgaca tgcaaaaaga gatcgtaaat
    18001 aacggtacac tctctccaca cactgcagca tctattctga caaacaatga tttgttagta
    18061 agaacattga aacgtaccgc tgccttgaaa gttttagtga acgtagacat ctccactttc
    18121 ccttatttga gtcaacaaac tgatatgggt ggaatgactg cttctgtacc tccagaattc
    18181 actgatttac ttttgaagga ttctttgaaa gatacagata atatgttgac tatcacaaaa
    18241 tacaatccaa gtgctttttt tggtacttct cttgatcttc aacttcgccg gcgtggaatc
    18301 gaaactatca ttttaagcgg tgtagcgaca acaaacggtg tatatgctac tgcattagat
    18361 gcctttcaac acgggtacca tatcgtgctt gctgaagatg cttgcagtga tcgtgacaaa
    18421 gaatcccatc aattgttcat aaaaaagata tttccgaaaa ctgcacgagt acgctcaacg
    18481 aaacaaataa tcgaagctat ccaacaaagc aaataagtac tggcacacgt gattttcatt
    18541 tacttctttt tgatttcatg ggaaaataac cacaggattt agataggagt atgaacatgg
    18601 tgaaaaaaaa gatcacaatc aaagaagtcg ctaaactttc cggcgtttca atcacaactg
    18661 tctccttgat attaaatgga aatacggaga aattcagtcc gaaaactgtt cagaaagttt
    18721 tagctgccaa agaagaatta ggctatcagc ctgactattt tgctagacaa atgattacca
    18781 aggaaacgaa aacaattggt gtactcgttc ctgatatcac gaatccattc ttcagtacac
    18841 tgatgcgagg aattgaggat atcttgtata agcagaattt cgtcacgata ttatgtaatg
    18901 ctgattctga tcatcaaaaa gaaatcgaat atctagccga gctcacacgt cgaggtgtgg
    18961 atggattcat tatcgcgact tctgctgtct ctacagatgc aatcaacgaa aatttgaaaa
    19021 aacaaggccg gcccttcatt gttttggatc agaaaaaatc tgaaggattt agtgatgccg
    19081 tacgaacaga cgattttcgc ggtggttacc tagcgggcat gcacttgctt agtctaggac
    19141 atcaaacgat cgcccttgtc tatcctgaaa atccaccgga aaatgtccac gctcggattg
    19201 aggggtttaa atctgctctt gatgtttacc aaatccctca cgaccaactg atcctcttac
    19261 ctacacagtt ctcaaaacaa ggtggctacc agatcacagc agaacttctt gattctgctg
    19321 caactggtgt gtttgcttta aatgatgaat tagcttttgg attatatcgc ggattagagg
    19381 aagctggaaa aagcatccct gaagactaca gcattattgg ctatgataat atagatatgt
    19441 gcgagtatat caagccgaaa ctgacaacga tcgcacaacc aatcttcgaa ttgggacaaa
    19501 catctgccaa gttattgtta gatcggatcc aattcccaga aaaagaatgg gaagaaaaaa
    19561 gattacctgt acgattagaa aaaagatttt caactgcacc cttgaaatag ctttcaaggt
    19621 gtgctatatt tttatcgaac tattaaaacg ttttactaaa acgttttaaa aaataatttt
    19681 cccatcaaaa taaatgagat agttggaggt ttgtaaaatg aacacggtta cagtaattgg
    19741 aagtattaat atggacacta cccttcgttt aaccaacatg cccaagccag gcgaaacgat
    19801 gcatgcgcat gagatttttc acgctggcgg aggtaaaggt gccaaccaag cagtggcagc
    19861 caaacgttca ggcgcacgga catcttttat tggaggcgtt ggggctgaca gcgaaggaca
    19921 acagttatta gatctactga caaaagaaaa catcgataca tctgggatag ctgaaatcca
    19981 aggcgccaca accggacaag caatgattat ggtagacgca gcaggagaaa atagtatctt
    20041 gatccatgca ggggcaaata atgctttcca tgaacaagaa gtcctaaaaa acaagcaact
    20101 gattacaaac agtgacttta ttattgctca gtttgaaagt agcttagatg cgacgatcct
    20161 agctttttct attgcaaaag acgcaggaaa aacaacgatc ctgaatcctg caccagctcg
    20221 tgaaacgatt cctactgaac tacttgaaaa gacagatatc attataccaa atgaaacaga
    20281 aacagaaatc attacaggaa tcagagtgac agatcataac agtttggtcg cagcagccga
    20341 aaaactccat gaattaggta ttggaacagt aatcattaca ctaggcagtg ccggcgcttt
    20401 ctatcataca gaaaaagaac acggtatcgt ccctgctttt aaagtggatg ccgttgacac
    20461 aacagccgca ggtgatacat ttattggcgc attaagcagc acacttcagc cagaccttag
    20521 taacttgaaa gaagcgatcc tttatggaaa tctagcctct tctgttgctg tgcagtctta
    20581 tggtgcgcaa ccttctatcc cttaccgcga agctttagaa caatcagcag tctaaaatta
    20641 acaatgagag gaagaaacaa acatgaaaaa aggaaaagta atcaattcag atatttcacg
    20701 agtaatcgca caaatggggc actttgataa attaagtatc ggggatgcag gcatgcctgt
    20761 cccctttggt actgaaaaaa tagatttggc tgtctcaaac ggtcttccaa gttttttatc
    20821 tgttttagaa aacgttttag aagaacttga ggtacaatcg attgaattgg cggaagaaat
    20881 aaagacaatg aacccggaaa tattggaaaa aatcaaatta ttgttaccag atacaccgat
    20941 tagctttatc ccacatgaag aaatgaaaaa agagttgaat aactgtcatg catttattcg
    21001 tacaggcgaa atgacacctt acgccaatat tatattagtg agtggagtgg tcttctaatg
    21061 gaaacgacaa tgaatcctga acagatggta ctgtccgagc agattcatat gctgattgaa
    21121 cagttgggag gaaaagacaa tattgattct gtccttccat gtgcgactcg gcttcgtatc
    21181 aaattgaaaa aacagccagt agccaatgca gaaaaattaa aacagcaaat cccttccatt
    21241 aagtatttca ttttactcga tagatatgca gaaataatca tggatcaggg attggctcct
    21301 gcttacaagg aattgacgcg tcaaatcgat cattcagctc cgaaagaaaa gctaaatccg
    21361 atcgaaaaac tatttggttt gatatcggaa atctttacgc cgatcattcc gctgtttgcc
    21421 ggttctggta tattaaaagg tctggttgtt ttagcgacta cgattggctg gcttagtgaa
    21481 acaagcggaa cttatcatat tttgtctgct gcgtcgaatg ccgtctttta ttttctgcca
    21541 atctttcttg ctttttctgc tgccaaaacg tttaaagtga atggatatat tgcggctgtc
    21601 attgctgcag cactgattga accgaatctg acctctttac tttcagacag tggcaaagca
    21661 gctactgatt ttctggggat tcctgtagta ctgatgcagt atacgtctac tgtcatgccg
    21721 gcgattttag gtattttctt ttattctttt gttgaaaaat tcctcaaaaa atatattcgc
    21781 gaaaatatgc aattagtatt tgtaccttta ctttcattag ttatcacggt tcctttgacg
    21841 ttaatggtat tcgggcctgc gggtgtttac cttggcgaag gtttagctgc cgcaattact
    21901 tggatgatgg atattaacgg accgatcagt ggcgcaatca tcgctggtgg ttggaatatt
    21961 ttggtaatat ttggtatcca atgggctgta aaccctgtca tgatcagtaa tatctctgct
    22021 tatgggttcg accgaatcgt tccgttgaca ggagcagcca attttggaat ggctggtgca
    22081 gctttaggtg tatttctgcg aagtaaacgt tcaaaaaccc gttcgatctc cggttctgct
    22141 tttgcttcga ttttacttgc gggagtgacc gaaccaactg tttacggaat tgcgatccca
    22201 ttgaaaaaac catttgttgc tgcttgcatt ggtgcagctg ctggtggagc tgtaatggga
    22261 tttgcccaag tgaaagcgat cgcttttgta tttggtagtc tgaccacatt gcctgcattc
    22321 atttccggta cttttttctg gtacttagca ggactggctg ttagtctagt cgtagcaatg
    22381 atcactactc tcgttagcgg gtttgatgaa gacctaatgt cttatgaata gttaatgaaa
    22441 aagactgaag tatgagatgt ttatcacata tttcagtctt tttcttttta ggatcattat
    22501 ttttataaat ttttgtgata ctttccaaac tttggtataa tttacatatg cataaccatt
    22561 tttatcccca cttgtttatc cccaaacaaa ggtggtctat gcaataaaat gaacgtcaaa
    22621 gaaaaacgaa tgaatgctcg ttgcttattt acaaattgtt ttatttagaa tacacagaca
    22681 gatttatgaa aagaaaaagc ttttcgacac gattatctgt tccaaaaaat gtctaatgaa
    22741 tgctgaataa gacagaaaat caatgaaagg aaaaggacta gtttattatg aaaaataaaa
    22801 aaacgatacg tttgctagct gctacttggc tgattgctcc gtctgttttg tcgaccaata
    22861 ccgtatttgc cgtagataat caaatagctg atcaaacagt cacaacagat acgactacat
    22921 ccagctcagc tactagtgaa acaacggatt cttttacaga agacccaact ggaacaacta
    22981 actcaacaac aggatcgacc gagccaactg aaattgatcc ggctgatcca tcagaaaatg
    23041 aaaagccagc cgagacatat tcccttacaa ttgctccttc tttgattggt aagatccaac
    23101 tgactgcttc gacagcggat tcccctgaac agcaaccttt ggatattacg gaaagtggaa
    23161 cggtagaagg tttattagcg gaaacgaagg taacttacac tattacgcca gctgaaggtg
    23221 aacaaattgc ttcagtcgct gttaatggtg ccaaagaagt aaattatgat gcaaagacct
    23281 ttacagtagg tacgaacaat ttatctatta ctgcagaatt taaagctgga ccggatactt
    23341 caacagagaa accaactgat cctagtgaag aagtccctag tacaccagat ccggaaacgc
    23401 caaacaaacc agatgtgggt aataacaaca ataataatgg ttcaaataat tcaaacaata
    23461 ataacaataa ttcgaacagc gggaattctt cgaatcaaac aaacccatct ggatcgaata
    23521 acaatggaaa tactcagaga gaaccaaacc atccaaacaa tggttcatcg aatggcggaa
    23581 cgaacaatgc ttcatctcgt ccaaatacag caaatcaaag cggtatagag aatccatctt
    23641 ctgcttcttc tgattttgtt gtaaaatcac caattgatgc tgtattgcct acaaatacaa
    23701 cgaatgtcca acaagcaatc gttcgagaag cattcaagca tcttggaaaa ccatatgtat
    23761 ggggagcaaa aggtccaaat acttttgact gttctggttt gacttattac gtatatatga
    23821 aagcaaccgg tcattatatc ggtggctgga caggcgaaca gcaatatgca ggtacgcaga
    23881 ttccagtaag tcaggcccaa cctggcgatc tcgtcttctg gggaccttct tccggtgtta
    23941 ctcaccatgt aggtatctat attgggaacg gacaatttat ccatgcgcct caaccaggag
    24001 ataaagttcg agtaacttct atttcagatt tcacgcctga ttttgctgtc cgagtaaact
    24061 tagcaggttt acctgctgct tcaggttcgc tgacaggcgg ctcatctatt ttagatggat
    24121 tagatggctc gttccatttc agtcaaaacc aaacaactga tcagttcttg aaaaagattg
    24181 cagatgatgc acaggaaatc gggcaaaaag aaggaatcta cgcatcagtc atgatggctc
    24241 aagctatcct tgaaagtgga tctggaaaca gcttattatc aagcgaacca aatcataacc
    24301 tttttggtat caaaggatca tataaaggaa gcagcgtgac cttcaataca cttgaacaag
    24361 atagttccgg acaaagctac caaatccgcg cacaattccg taaatatcct tcttacaagg
    24421 aaagtttgga agactatgcc gacttgatca aaaatggact gactggcaat cctgatttct
    24481 acaaaccaac ttggaagtcg gaaacaaaag actacaaaga agcaaccaaa tatttggaag
    24541 gacgttatgc tacagatcgc caatattcac aaaaattgaa cgcgatcatc gaagcgtacg
    24601 acctgacaaa atatgatgaa ccgaaaaaag aagcagcaac aactgaagaa gcaaacgctg
    24661 aaatcctcaa tatgtctaag cgattcgatg tgccaatcac aggattagaa ttttcaacga
    24721 taaaacgcgt caacaacgct tcttccatct tccgtgtcgc ttcaatctgg gatttgtgga
    24781 acaattttac ggcaagacag ataccagaaa caactactcg tacagttaaa gctgcaactg
    24841 ttcctgtact aaccttgtta agtttagaca aatcattctg gcttgatctg cctttaaaga
    24901 aatagaataa attaaaaagg ggatgtgaca ctgtcacact ccctttttaa tttattctat
    24961 ttctttatat ttattttgta ttccgttaca aactcgcgta cttggttagg ttccagagtg
    25021 acatctccaa aactttcaag tcgctccgct cctggcagtt cttgagtctc aaatgtgata
    25081 ccgccatgat ttactaattt attcccgcgc atttccggtc catcttctcc aaaatttgct
    25141 gtaaaaatca cgattcctga agcatctgtt tttacttgga tcgatacttt ttcatctggg
    25201 cttgtcaatt ccgcagcatt tagtaaacca gattgttttt tcaacaaaaa tggatgatcg
    25261 atgccgccca ctatcattga ttggcgaaaa tcactggcaa agacctcacc taatttttta
    25321 ggacgttgga agtcaaatgc tgtgccactt actgaaacta cttctcctgt aggcgttgta
    25381 tctttattga gcgtagcaaa taatcggcta ttcaaccaaa gtatatgttc gtcgattgac
    25441 tgtgctggat caccggttaa attgaaatag acatgatttg taggattgaa tactgttttg
    25501 cggtcactga tcccttggac cgtcactcgc catgtatcat cgttcgtcag cgtataccgt
    25561 acttctacat ctaaagttcc aggaaatccg ttttccatat ctgggctagt ctgatagaaa
    25621 atgaccgatg cttcgttttc accattgatg actgtataat gccactgctt ggcttcaaag
    25681 ctaggtgcac caccatgtaa acaatgacca gtgtccgggt ctgtttttac ttcgaacgtt
    25741 tcatcatcta atttgaatgt gccattcgta atccgtccag ccatacgtcc gattgaagca
    25801 ccgatataag ggtctttttc tagatattct tttgcttcat caaaacctaa gatcaactct
    25861 cgtttttcat tatcgattat tgtttgaaaa tgaacaaccc gtgccccaag atcactgaca
    25921 gacaaagatg tcccattttg attcgtgatc gtaatcaagt gatagttttc gccaaaattt
    25981 tgttcattga tttccatgtt ccttgcaccc tcttttcttt catttgcact gattccagta
    26041 tacatggaaa ccgttttctt aacagcttat tccgtgagta acgagaaaaa atttactata
    26101 ttctaataaa agaccgtgaa aaaaaggttg tgacaaaaat attgtcacaa cctctttttc
    26161 cgaacaaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaaccaa acgaaaaata
    26221 tgaggagcta tttttgcttg gctgttctta ttacttgatg caggacagct ttttcacaat
    26281 ctctttttca atacgattcc agtatataac catgtcgtca gtacatagta gatgaaatac
    26341 aatacgaaga agatcgtaaa cggaacccaa atatctttgt atggattttg catgaataga
    26401 ccaaacattt gtaatccgaa taagacatgg actacaccta agattcctgg tgctaagaat
    26461 aacacgccta cttctctacg gatcgattgt ttaagcaacc cgcgtcgtgt tcctatcttt
    26521 tcaagcattt cataacgagc aatgtcactc ttgcttcctg acaagatctt gaacatcaaa
    26581 cagctagcaa gcatggtcaa gaaagctaaa cctaggaaga atcccataaa ttcaaaacca
    26641 gagaatagac cattatacac ttcatacatt tcatatttct ggttgctgta ctctactttt
    26701 tgcaaagatg gattgttcat tttattttga gcaacaagtg tttcgattgg tttcaggttt
    26761 gcttggaagt cttttacctg aactagctgt aaagtggatt gtggcaagtt cagttgatca
    26821 aattctgatt gactgactac cttgatttct ttttcttgct gttccggcaa ttcatagctt
    26881 cgaagttcct cttgtacagt cagttcattc atttttttag caggaacttt ttctttcgtg
    26941 atcttcggcg gtgttccatc ttcatgtttt atcatgatca atggttgagc cgcaaattca
    27001 cttgcattat agtaaatcgt tcccgcatct tctttttgag tataagaagc agaaagtgtt
    27061 ggtgaaagtt ctttgatttt ttgctgatct tctgcccgag gattattcat cactagatca
    27121 tatccagaat tgttttctgt caagtctgca atttcatttt taaaacctaa tcctaccgtc
    27181 aaagcgccca gtgccaaggc aaacagcata gcgaccattg agagcatttg tgtatattca
    27241 cgaatacgaa agtttaactg tgacaatgtg aaattgttca attttttcaa tgaaatctgt
    27301 tctgatttct ttagtaatga taaaaagaaa atgatcacag aatggaaaat gaaataagtt
    27361 cctaatacga tcgtaactaa ggctacccct ataccaaaga cttgataggt ccccacgtgt
    27421 ttcatcatat agtaaccgca gcctagaaaa gcaattccca gtactacttc aaggaaaaac
    27481 aagaatttat gctgcttgat tcttgttggt gtctgatccg cacgaatcaa tgctaaaatc
    27541 ggttttttga caatcgaaaa agcattgaca acagcagcta ataaaaacaa aacagcaaaa
    27601 aacaaaacag tgatcaacaa cccgtgaata ctaaaaggtg taaaatgcga aatattgaga
    27661 tctaactgat ccactaataa tcggtggacg atacttgtca atcctacacc gacagctgaa
    27721 cccactaaag tagctgcgat ccctactaag aatgtttcag aaaaaatcat ctgtgcgatt
    27781 tttctgcctt ttgctcccag catcataaac attgcatagt ctttttgccg catcgtcatc
    27841 aaaaaggaat ttgcatataa aatataaacg aatgtgatga tccctagtaa aacagaaccg
    27901 aatcggaaaa tgataactgt agccgcaacg atcgtattac ttttcaaaaa agcttcgttg
    27961 cttgccattg attcaaacat gtaaaaaata gcagatgcca taactaatcc tgagaataac
    28021 accaagtagt ctcttaaacg gctcttgatc ccagtaagtg ataatttcca aagcatagtc
    28081 aatctccccc tactgttcca agtttccaag aatagatagt atttctcgat agaaggaatc
    28141 acggctttga tctccacgtt tcacttcttg atggataccg ccatccttga tgaataaaat
    28201 acgttggcaa taacttgcag aaaacggatc atgcgtcaca agaagaatcg atacatgatc
    28261 ttttgtattc aattcgtcca tcgtatctaa aagatcccgg gcacttttgg aatccagcgc
    28321 acctgtaggc tcatccccta atagaatcgt tggttgtgtg atcaatgcac gtgcggcggc
    28381 gacccgttgt ttttgtccac cggaaatctc agaaggatag ctttccaaaa tatgagagat
    28441 ggataaacgt tctgcaattt tttggacacg ttgcttgatt tcttttggtc tgacgccttg
    28501 taaagaaaga ggaacagcga tattttcttg tgctgtcaag ctttctaata aattgaaatc
    28561 ttggaaaata aagccaatct cattcgcacg aaaatctgct agttgatttc cttttaatgt
    28621 ggtcacatct ttttgattga ttcggatatg cccatctgtt ggtttatcta atgtggataa
    28681 aatattcaaa agtgtcgact taccagaacc agaagcgccc atgatcccaa taaattcgcc
    28741 tttttctaca tcgaagctga tgccgcttaa tgcttgcgtc tttttctcat tgttttttcc
    28801 gtatactttt gttacattct gtacttcgac tattttgttc atttctttcc ctacccttta
    28861 attagttttt ccagtgctgt tttcggcacg tctttttttt cgacttcttc atacgaagtg
    28921 acacctttat ttttgttcca ggtgattttt aaatatgctc ctttacgaag cggtcgagtc
    28981 aattgtccct tgaagtccag ttctttttcc ttaccctctt tcgtatagcc aggcaggata
    29041 tatctatagt tgactgtttc ttgtccagag tctgattttg aaactgtttt tgtaccatct
    29101 gtcgtaattt gtacatagta ttcttctcca cccataacta cgtaatctgc gattttcacc
    29161 cctgcaagta ccacaacgat gaaaacaagt agaccgatca gaagtttttt cactttttcc
    29221 ccttctctct tactttatat actgattata gaaaaaagcg aacaattctg ccttcgaatc
    29281 aactatcatt cttctttcaa ttttgtcatt ttctttaaaa caactaaaaa aagagttcaa
    29341 gacaataatc cgtcctaaac tccttcttcc tatcttacaa aaagtatgtc acttacggtc
    29401 tttcatgtcg cctgtattga taacaggttg cgttcctttg tctgtaatga ttgagacaag
    29461 taaattattg atcaattgga ctttccgatc atcggtgaaa ttgatttctt gtccatcttc
    29521 gatttgctct aaggccattt gagtgattgt gacagctcct tcaacaatgg tttgtcgagc
    29581 agacaaaatg gcttttgctt gttgacgttg gagcatggca ctagcgattt ctgttgcata
    29641 ggctaaatgg ttcaagcgtg tttcgattac ttcgacacct gctactgcta aacgctcttg
    29701 cagctctttt tgcaactcgt cagacactgc cgtcgtgttt cctcgaagcg tcaaatcatt
    29761 ctcattgaat gtatcatacg ggtattggct agcgatatgc cgaatggctg tctcactttg
    29821 gatttctaca aaatcttgat aatacgctac atcgaataat gcctttgctg tatcaacgac
    29881 tttaaagact actactgctg agatttcgat ggggtttcca tccaaatcat tgacttttaa
    29941 aacagaacta ttgaaattcc gaactttcaa cgaaacagtc attttttgtg ccaaaggtat
    30001 cgtaaggaaa aagccatttt ctcgaatcgt acctaaatat tgtccaaaaa acaaaattac
    30061 ttttgcttga ttaggactga ctacagttgc cgaacttaac aacaataacg tgatcaacca
    30121 aagaaaaata cttaaaaaaa gtgcccaaac tttcgcttgc cacataccta aatagaagaa
    30181 aaacaatcca actaaagcta atacgaccaa tccgacaatc cctgcataac cattgatata
    30241 aaatgctctt ttttctctca tgctacttcc tgcgctcctt tatccttcaa taaatatcta
    30301 atgttacttt tttaattttt ttttcgaata cgcgccatta caccttctag ttctaactct
    30361 ttgtcttcag tgatgtacca accgcttgcc tgtgtccctc cggctgcctg cggtaaataa
    30421 tccaattttg ttcgaaccca gtgcttttct tctgtaaaat agtcttctac caagacctct
    30481 agatccatcc ctgctgaaag aggcaaatct tcccgaacag atttaaagat aaatcgttcc
    30541 ttcttcccat catacttcaa tattcctgta tcgataatcg gtaaagaata ataatccagc
    30601 tctttcttca ccgtcaataa atgtttgaaa acttcatggt acacttgctc taattggcga
    30661 ccttctgaat tgttcgggta attaaccatc actttttctt tgtctggatc atagaaggtt
    30721 tggcttagtt gagtgatata agtatgtaat ctttccattt ctgtttttaa cttttctctt
    30781 tgaccttctt ctaccttcat atactctttc accctgtttt ctttttagat attttctttc
    30841 ttattttact atagttatgt gcgttaagcg atgaaaaaca gcaaagattg gaaggatctt
    30901 tgctgttttt tcttaaaata tccagttgag tacaggatca atctgtttca ttgtcctctt
    30961 gatccgtcgt aatattgatc gatgtgtctt tagcagtgat ctttagcttt ttctgtcctt
    31021 gttctaacaa agtatttgtt attggctggt agtctttttc aatataagat tcaccagacg
    31081 tttggatagt atagccactg ttttccatct catgcaaaga taaattgcca ccgtttagga
    31141 tgacttttcc ttctttgctg atcaaactgt cttctagatg gaagttgctg ttttcacctg
    31201 tgacgatcaa gttatccaca ttggcctcat ctaatcgaag tgagcctaat tcatctttcg
    31261 cagttattgt tcctcctgta aattcattga acgataccca gccatcatgt gtcttagtat
    31321 tcactttttt gattgatact tcgttgatat caattgcact attattccct tcgattgtca
    31381 attcagataa ttcatgatct gcagggacaa caatagtgat ttgtgcactt gtgtcttcta
    31441 aactaatgat tgcacctgtt gattttttga ctgcattcac agtcaacacg ccatttttct
    31501 cctttatttc tggcttttct gtcttatcat aagtcgtttt tatctcaaaa tccaatccgc
    31561 gtatgaccga aactgtttga ttttctgcat ggatgacgat tttgtttgca tccttgattt
    31621 tttttgtctc agtaaccata tcgatcaacc gaggaccatt atcccaagtc agactctttt
    31681 gagcgcctaa tgtataagtt gcacctgcta ggaggccacc agtaagcatc agaccaatac
    31741 cggtaatcaa aaaatgtttc aatctcaatt tatgtgtcct ccttcaaacg cccgctgaat
    31801 gcagatctct ttttgtaata aaacgtcgtc ccatccattt aacaaagttc atcatgacat
    31861 aaaaaagccc ttttgttcct ttcagcaaca gtggcgaaat aaatactgcg cttcctgtgg
    31921 cgaacagacc gacaccgata aagaataatc cactcagaat cgattgtcca aacacactga
    31981 ttcctgagat gatcatgaat aggccaacac ctaagatact gatacataaa acatagatac
    32041 taaaaatgag cgctagtaaa gcaaccaata gtcccccaat aaccaatata aaccccagtg
    32101 ctagtggaat gaggacagga gaagcaaata aaccgataat gattaaccaa atcaggcgaa
    32161 tttgtctttt tggatttttc actaactcgt tttctgcttg ttcttcttct gaaaagtaac
    32221 tgatttttaa ttttaaggca aactgctttg gtgtaccata ttcatcaatg atctgatcta
    32281 atgtttttcc agaatctaaa aaatattcct cataatactg gatcaaatca aaacgatcgt
    32341 cttcaggaat ttcttgaaga tgatccacaa cttcattcaa atattcattc atttgtgctc
    32401 tcctccaata accggtctaa agaatctcga aaaacaatcc attcatcaat aatttcttct
    32461 aaatgacgac ttcccattgg tgtcaacgag taatatcgac ggtttctgcc ttcaaaaggt
    32521 tcatcatagg tctcaagata tccttctttt ttcaatcttc tcaaaaccgg atatagtgta
    32581 gattcactga cagagagact ttcttgtacg atttttgtta aggcataccc atacatatct
    32641 tccttgctta atgctgctag cacagtacca tcaagaagaa cagttggaat ctggatgtgc
    32701 attacgtcac cttctattct caagtgtaat attttatttc aatctatatt atacaacata
    32761 taatatagtt tcaaaatcag tttgtcaaat acaaaaaaac tgactaaaag atttgatcct
    32821 ttttagtcag caaaaaatgg cttctctttg atttttatta taacagtggc ataatccaat
    32881 ttgtccacat ccagctcagt ggcatgataa cgatcattgc aggtatcatg tcagctactg
    32941 ggaacatttt cacgttgatc attctaaaac cggtagcgac catcaagaat ccgccgcaag
    33001 ctttaaaatc taagatcatg tctggtgtag tcaatggata aataaatttt gccaataaga
    33061 acaaaatata aaaaataatg aattggggaa ttgctatcac agaaacgaca taccctaaat
    33121 tacacgcaaa aatagctgct gtgaaaaaat caaggacaga tttagaaatc aaaatcgtac
    33181 tgtctcctgt catcccagaa tctaaacttc catagatccc cgtaccactt gcacaaaata
    33241 gaacaatgac cgtcactaat gtgctgataa attcatcgtg ggtcatacca agcttttcac
    33301 tagggaagat tttagaaata ggtacttgca tcagcgcagc ccccttattt atcccattac
    33361 ctaaatgaac aatcaaacca atcgctgttc ctatcactaa agcaaaaata acagccggca
    33421 tatttttcat cggcgcaatg gaatagattc ccatccccat cgaacacaca ccaaaaacca
    33481 tcgtcagctg tgctttaaaa tgttcactta atttatttcc tactaatccg ccaattagtc
    33541 cgccaaacaa aacggaacct acattgatca aaactccagt tggcatcaaa aaactcctct
    33601 ctttttctac gaagcgaaaa aaatcagaaa aatttctgta attattgctt tcgctgcaag
    33661 aaagattgta acagacagag aagattacac caattcattt tttgtgttaa agtattcttt
    33721 attggaataa gtaggaggaa tatgatggat atccataaat tcgaagtttt tcttgattta
    33781 gccgaaacaa tgaatttcac caaaactgct gatcgacaat tcaccaccca aggaaatatt
    33841 tctaaacaga ttctttcgtt agaaacagaa ttagacgtca aattattcga acgcgcccat
    33901 agaaaaatcg aattgaccga agctggaagc ttattgctcc catatgtaaa aaatgtcgta
    33961 gaacaatacc atgctatgca agaagtgctt gaaacctata cgaaagacaa caatctttca
    34021 ttaaatatct taacgatacc gacaatgatc aattataaag gtttttccaa aatcaccgag
    34081 tttttgaaac gccatcctga atttacagtc cagttgaaag aagtggaaag tgtcgaatta
    34141 acttacaatg acgccaatga acatagtgac acgatccact ttgctagaag tttccaggcc
    34201 ccaccaagtt cgattgagtg gttgcctact gaaacagacg attttgtagc agttctacct
    34261 aaaaatcatc ctttagctag taaaaagaaa ttggatctaa gcgaactgaa aaaggagaac
    34321 tttttgctcc ttggccccac tacgaacttg tatcaacctg tactagatct gtgtcgcgaa
    34381 gctggatttg aaccaaaaat ctcttataaa ggagcaagaa tcgacttgat catcaatatg
    34441 attgcaaatg acatggggat tgctattgtt atggaaaaaa cagtcaagaa tctattaaaa
    34501 gaaaatactg tgattctccc tatttctcca acaacagaga gttatcttgc atttactcgt
    34561 aaagtcggtg agcattcttt ggcttcagat acattttggg agtatttaaa tcaagaagaa
    34621 aactaagccc tacattttct gtccatctat ccacccaaaa aaagagagcg acgcatgact
    34681 ttcgctctct ttcttgcatc atgtccttgt ttcaatgaca cctctaacat ttctcgtagt
    34741 tcaataagaa agcagcgatt tttatgccaa aagaaccgta agaacatttc cctaatcaca
    34801 aaaaaacgac taaaaacccc taaaaacctt caatagaatt tttaggggtt ttattctttt
    34861 tcctattgtt tacttaaccg tatcattgct gtcgttccta ctccttcgat actgctataa
    34921 tcaatacgtc catcatgcag ttctagaatt tttttaacca gtgaaagtcc tagcccattc
    34981 ccagaaattt gacgacttgt atctccttga taaaattttt caaacattcg atctctggtc
    35041 tcttcattca ttcctacacc tgaatcggtc acttccagta caatctctgt ggctgtctca
    35101 aatagtttga tatgaatctg tccattgatt tgattgtact ttatggcgtt atccatgata
    35161 ttcagccaca cttggtacaa aaactcttca ttgcctgtgt aatttactgc agcaagttcg
    35221 atatccaatt ctagctgttc tttctcccat ttaggctgaa aaaaaacgat tacttctctg
    35281 atttgttcat ctaatcgata ctctttcttt tccagttgga tacgctggtt ttccagtttg
    35341 ttcaatttta agatgttctc tgttaattgt gaaagctgcg tgatactttc gataatccga
    35401 tataaaaata tttggcgttc ttcgtcagag agatttgggg cttgtaacaa ttgaacatat
    35461 ccttggattg ttgctagtgg tgttttgaat tcatgggata cactggacac aaagtcattc
    35521 ctcagtgtct cgatactatt gagttcttgg accataacgt tgaaatcttt atacagttgc
    35581 tgtacttctg ctactttctg ctgttcatcc attcgaatcg aaaaatctcc cgttgctacc
    35641 agactcatat ttgtccctag cgcactgatt ggatgcagga tacgccgccc aacaaaaatc
    35701 gatattcctg tacccacgac catactgaac cccgcaaaga cgaataacgg gtataacgtt
    35761 ggttttccag ccgattcagc aggcgagata gaatgtcgag acaataagaa aataaaaccg
    35821 agaaaacaca gcagtgtcac taagatcgaa atgaagacaa tactagcaaa atagatccat
    35881 aattgtgaaa atagcttttt tttcatcttt aaataaccgc cttatagcct agtccccgta
    35941 ctgtgatgat ttgaaaatct tccgtctttt caaaacgggt ccttaaacgt ttgatatgta
    36001 catcgatcgt tcgttcttct gtattcgtat ctaatcccca aatatcatcc atcaactgtt
    36061 ggcgagtgaa gatcttgttg ggataagaca gtaatttata cagaagcaga aattcttttt
    36121 gaggcagaat caacgaatgc tgatcttgta acactgtata agcatctagc tctaattttg
    36181 tttgtccgat tatcaagcta ctgctgtaat tgatctttga acgtcgaagc aatgcttcca
    36241 cacgtaggat catctcattg acatcgatgg gttttgtcat ataatcatct gctcctaatt
    36301 tgaacccgcg tttcttatcc tcaaaatcgg cctttgccgt aatcattaga acaggtgtgt
    36361 caaatctggt gctacgtaat gagtctagca agtcatagcc atccattttc ggcatcatga
    36421 tatctgtaat gattaattct atacgtgtcg cttctagttt tttcagcgca tcctctccat
    36481 tcaatgcggt gatgactgaa aagcccgctc gttccaacac agaacggtaa agcaactgta
    36541 attgcggatc atcttctact actaaaatac gattcattga tctccctctg ttttttgaaa
    36601 aatttctaga ttttttcttg tagttcatct tcagttaaca tttatttcgt atactacgat
    36661 cattgttctg atatacttat tagtataccg aatatttcaa tacgggagaa gggtgattat
    36721 gcttcaatta aaagatataa aaaaatatta taaagtcggc gaaaccacca ccaaagcatt
    36781 ggatggtgtg tctgtcgctt tcagacaaaa agaattcgta gcgattttag gtcctagtgg
    36841 ttctggtaaa acgactatgc tgaatgtgat tggtggacta gataattatg attccggtga
    36901 tatggtaatc aacggaaagt ctactaaaga ctttaaggac agtgactggg atgcctaccg
    36961 aaacaactct atcggtttca tttttcaaag ttacaacttg attggtcatc taggcattat
    37021 tgaaaacgtt gaacttggga tgacattaag tggtgtttct aaagacgaaa aacgaaaacg
    37081 ggcagaagat gcattgcacc gagtcggctt gacaaatcat atgcataaaa agccaaatca
    37141 gttatctggt ggacaaatgc agcgagtggc catcgctcga gcactagcca atgacccaga
    37201 tatccttttg tgcgatgaac caaccggagc gttagataca gaaacaagta tccagatcat
    37261 ggaattgatc caagagttat cgaaagaaaa attagtgatc atggtcacac ataatcctga
    37321 actggcccat caatacgcag atcgaatcat tgaattttct gatggtaata tcttaacaga
    37381 ttcccatcct cacatcgaac gaccaaaaga tgatcaattt aacttacgca gaacaaagat
    37441 gagtttttgg acagcgttaa agctttcctt caacaatatc cgaacaaaaa aaggacgtac
    37501 tttcttgacc tcctttgctt ccagtattgg gatcatcgga atcgcgatcg ttctgtccct
    37561 ttcttctgga ttccaaaaac aaatcgataa cacgcaagct gaaacgatgg cgaaatttcc
    37621 gattactatt tctaaggtaa cgactaacca gactcgtgac gatgccggat tgggtgcaag
    37681 caaagcggat tatccagaca gcaaaacgat tactgcaaaa gtcagcgaag aagataaagc
    37741 acagcacacg aataagattg atcaaacata cgtcgattac gtaacagaca tcgatcctaa
    37801 cttaagtaat aatatcggat ttacccgtac tacaggaatc aatcttttac gtgatgtaaa
    37861 tggcaaagta cagcctgtca gcttctcgaa tcaaaatcct gatacggaat ctctttcttt
    37921 atctagtacg atgtcagcaa tgacaggtgt gggagtgtca agtttcccta cacagcttga
    37981 cactagcaaa gaaaactttt taaaagacaa ttattcttta cttgccggaa gttatccagc
    38041 ctcagcaaca gatgtcgtgc tgatcgtaga cggcaataat aatactaata tcaacgcatt
    38101 gaagaatctt ggatttgatg tgaaagaaga cgaaaaactt gattttgatg atatcgttgg
    38161 tacaacattc aaattagtaa acaacaatac ttactataca aaacttccaa ctgggaattt
    38221 tatccctaat acagattatg atgcgatgta tcagaatgct tctgatgaat tgaagatctc
    38281 ggggatccta cgcgtgaaat cttcttctac aatgaatctc ttatctccag gaatcgctta
    38341 tagcgatcaa ttaacgactc aaatcgtcaa cgaaaacaaa gaatcagaaa tcgtcaaagc
    38401 tcaaagagac agtggtgtaa atgtcttaac tactgaaaaa gttgacgaaa atgccaaaca
    38461 aacattgctt agttatttag gtggagacag cttgccttca agtatcatga tttatccgaa
    38521 caattttgaa gacaaagaaa agatcttgga ttatctagat gattacaata aaggcaaatc
    38581 agatgaagat aaaatcattt atactgattt agctggaacg atgactgaac tgactggcgg
    38641 attgatggat gctatcactt atgtccttat tgcttttgca gggatctctt tagtaacaag
    38701 tatgatcatg atcagtatca ttacttatac atctgtgatt gaacggacaa aagaaatcgg
    38761 tgtactaaaa gctttaggtg cacgtaaaaa agacatcact cgcgtatttg acgcagaaac
    38821 gtgtatttta ggtatttctt ctgggatatt aggtgtgttt attgcatggc tagctacgtt
    38881 ccctatcaat agcatcttat acaacatgac ggatctaaaa aatgttgctc aactaaatcc
    38941 tgtccatgcg attattctag tgatcgtctc tactatattg acgatgcttg gcggtcatct
    39001 tcctgcacga atggcagcga aaaaagacgc cgctatcgct ttgcgtgcag aataaggttg
    39061 tgacaaccac atttagattt gagtcataag agcgctgaaa tagtagaaaa cagcaagaaa
    39121 cgaggaagta atatgttcct tgtttcgcgc tgtttttttt aattattttt tataattctg
    39181 gtatactttt ctttcctttg taccacaact cttctggcgt atacctgtct tttccattta
    39241 tgaatactgt tccatctgct tcaatcgtta cttcagtccc aatccacata ttacgagaaa
    39301 gtgcgagaag attttttctt tttcctcttc ctttttttcc aaattgcaag gcatcggtta
    39361 tctcttctgt ccaatgagat ttcacgtatt ttacttctcc gttttcaaat gtaactttca
    39421 aaatattcgc tatattttcc acttttactg ctttaggaaa tgactcctcc atttttttca
    39481 ctttccttcc tccaacacta agtatgcgct ttcttttagt gataagtata tcatagttta
    39541 ttctattatc aaaaaaacgc atccattcca ttgtagaata ctttaccgaa caagttgaat
    39601 tgttttcctc tttttcacct tttactatag ataggtaaag acaactaagg aggaatctct
    39661 catgacagat caaccgtatg atttaagaaa agtcatcgaa gaactaaaaa cacttccagg
    39721 tcaatatcac gaaacagata ttgaaatcga tcctgaagca gaattatctg gtgtttaccg
    39781 ttatatcggt gctggcggta cagtaaaacg tccgactcaa gaaggaccag ctatgacttt
    39841 caacaatatc aaaggtttcc ccggcacacg tgtgaacatc ggaacaatgg ctagtcgtaa
    39901 acgtgtaggt gaaattttgc accatgatta tcgaacactt ggtcgtttct tgaaagacgc
    39961 tgtagaaaat ccaatcaaac ctgtgaaagt agacaaagca gacgctccag cacaagaagt
    40021 cgttcatcta gctaccgatg cagacttcga tattcgcaac attttgcctg cacctacaaa
    40081 tactcctgaa gatgctggtc catacatcac aatgggtgta gtcttaggat cagatccaga
    40141 aaaaacaatg agcgatgtaa cgatccatcg tatggttcta gaagataaag atacgatcgg
    40201 tatgtacatc atgcctggcg gacgccacat tggtcacttc caaaaacaat tcgaagcctt
    40261 gaataaacca atgcctatca ctatcaatat tggtttagat ccagcaattg ccattggcac
    40321 gactttcgaa ccacctacca ctccacttgg ctacaatgaa ctatggtgtg caggagcttt
    40381 gcgtaacgaa cctattcaaa tcgttgatgg tatagcagtc gatgaagtgg gtattgctcg
    40441 tgctgaattc atcattgaag cagaaatcat gccaaatgaa acaatgcaag aagatatcaa
    40501 cacgaactca ggaaaagcaa tgccagaatt tcctggctat actggggatg ctaatccagc
    40561 tgtacaagta gtgaaagtca aagcagttac tcaccgaaaa gacaatccaa tcatgcaaac
    40621 aacgattggt cccagtgaag aacacgtatc tatggccggt attcctacag aagcaagtat
    40681 tcttgccttg gtcgataaag ctattccagg aaaagtagta aatgtctaca atcctcctgc
    40741 aggtggtggt aaattgatga ccatcatgca aatccgtaaa gaaaacgaag cggatgaagg
    40801 tattcagcgt caagcagcta ttcttgcttt atcagccttc aaagaattga agacagtcat
    40861 tttagtcgat gaagatgttg atattttcga catgaacgat gtagtttgga ctttcaacac
    40921 tcgtttccaa ggagataaag atatcatcgt gatcccagga atgaggaacc atccattgga
    40981 cccatcagaa ttaccagaat ataatcctgg ctctattcgt ttccgtggga taagtgcaaa
    41041 aactatcttt gacggtacag ttcctttcga tatgaaagaa cattttgtac gtgcaaaatt
    41101 ccaagaagta gaagattggg aaaaatattt gaaataatga atgatctgca actgttttga
    41161 catcataaaa aggagtgcgg aaaatggaac aattggcaca acggaagaaa aaaatcgtga
    41221 ttggtgtaag tggcgcttca ggcaccattt atgccatcaa cctagtgaag aaactaaaag
    41281 aatatccgat gatagaaaca cacggaatca tcagtccatg ggcaaaacag aacttaaagc
    41341 tggaatccga cttatcacta actgaattcg aaagcctgtt tgattaccat tattcaaaca
    41401 aagatttagg tgctgctgtc gccagcggat catttttgac cgatggaatg atcatcgtac
    41461 cggcaagtat gaaaacagtc gcaggtatct ctgttggttt aggcgacaac ctgattgcac
    41521 gtgctgctga tgtaacaatg aaggagcaac gaaagttagt cattgttcct cgtgaaacgc
    41581 cattaaatgc cattcacttg gaaaacttga ctaaattagc acgactaggt gtgcaaatca
    41641 taccaccaat tcctgctttt tataatcagc ctcaaacgat ccaagatctt gttaatcacc
    41701 atacaatgaa acttttggat caatttgaaa ttgtaaatga gcattcaaaa cgttggcagg
    41761 gtgattaata tggaaaaaga aacgatagtt gaagtaacca aactaggttt cacgatccaa
    41821 gctgctgtta aagtaatcgg taaagactta ttgatcactt taacaggcgg agatacacca
    41881 catatcggaa cagtcactac attttctcgt gatacagaga tgcaaactgt acgtttccct
    41941 agccacgatg gtcggttcca taaagacgat gtcttagccg aaaaaatagc tgtgatcatc
    42001 aaaactgccg ttccgggaag ttgtacgatc acttccggta tccatgttga ccatatctcc
    42061 aatgcacaaa tcaacgcctc ttttccgatg tcagaagagt taggttacaa gattttagat
    42121 tggttggaaa aaatagattt ccatatcaaa gaaccagttt attataaaga tggtgaaaaa
    42181 ccattatgaa agaagaggtt gtgaaatcag cgttatgacc tgagtatgag agaaaaaaat
    42241 ggaaaaatag cttctcatat tttgccgttt ttgagctaat accgagggac agctgattga
    42301 acgccgttta tcaagaggtt gtgataaagc cgcttcgttt cgagtagtaa gaaaaatttt
    42361 gtaaacatgg gtttccacat ttttgcataa atttgactta ctaccgagaa actggctttt
    42421 gaacaccgtt tatcaagagg ttgtgacacg tcttttgtca caacctcttt tatttttgct
    42481 tcactgtaac tgctacaata tcaccgattt cttttccgat gtttttacga atatcttttc
    42541 gtagtccaag gatgtagcag actgaaccat ctggattttt cacgcccata ttcacgatac
    42601 tgccttcata cggcacattg tcaaaagttg cttggacttt aactcttcca gcaccgaatt
    42661 cttttcttat atcatacgga aaaataatat atgccccgcc cttgccgact tcggaagcat
    42721 agatttttga ttcaaaagaa taaatttccc tcattttatt tctcctattt tttacatcta
    42781 gtatagcata gcaaaaaaaa gaaggaactg tgacaaaagt cttgtcgcag ttcctttttt
    42841 cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatagt caacaaacat
    42901 gagaagctgt tttttgaaat tgctccttgt gactcagagt taaacggcag gctcacaacc
    42961 tcttcct
//
LOCUS       DACDYI010000018        40681 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.018, whole genome shotgun sequence.
ACCESSION   DACDYI010000018 DACDYI010000000
VERSION     DACDYI010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.018"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            33..656
                     /locus_tag="IWM52_000922"
     CDS             33..656
                     /locus_tag="IWM52_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973194.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1105..2154
                     /locus_tag="IWM52_000923"
     CDS             1105..2154
                     /locus_tag="IWM52_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9973195.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2151..3032
                     /locus_tag="IWM52_000924"
     CDS             2151..3032
                     /locus_tag="IWM52_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9973196.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3035..3805
                     /locus_tag="IWM52_000925"
     CDS             3035..3805
                     /locus_tag="IWM52_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9973197.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4084..4938
                     /gene="aroE"
                     /locus_tag="IWM52_000926"
     CDS             4084..4938
                     /gene="aroE"
                     /locus_tag="IWM52_000926"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAP9973198.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5116..6279
                     /locus_tag="IWM52_000927"
     CDS             5116..6279
                     /locus_tag="IWM52_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP9973199.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6396..6806
                     /locus_tag="IWM52_000928"
     CDS             6396..6806
                     /locus_tag="IWM52_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP9973200.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6980..9208
                     /gene="topB"
                     /locus_tag="IWM52_000929"
     CDS             6980..9208
                     /gene="topB"
                     /locus_tag="IWM52_000929"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9973201.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9346..9663
                     /locus_tag="IWM52_000930"
     CDS             9346..9663
                     /locus_tag="IWM52_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973202.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            9823..10059
                     /locus_tag="IWM52_000931"
     CDS             9823..10059
                     /locus_tag="IWM52_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9973203.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10056..10454
                     /locus_tag="IWM52_000932"
     CDS             10056..10454
                     /locus_tag="IWM52_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP9973204.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(11121..12065)
                     /locus_tag="IWM52_000933"
     CDS             complement(11121..12065)
                     /locus_tag="IWM52_000933"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP9973205.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(12361..13163)
                     /locus_tag="IWM52_000934"
                     /pseudo
     CDS             complement(12361..13163)
                     /locus_tag="IWM52_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            13384..14598
                     /locus_tag="IWM52_000935"
     CDS             13384..14598
                     /locus_tag="IWM52_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9973206.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            14620..16635
                     /locus_tag="IWM52_000936"
     CDS             14620..16635
                     /locus_tag="IWM52_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAP9973207.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16718..18091)
                     /locus_tag="IWM52_000937"
     CDS             complement(16718..18091)
                     /locus_tag="IWM52_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP9973208.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(18117..18401)
                     /locus_tag="IWM52_000938"
     CDS             complement(18117..18401)
                     /locus_tag="IWM52_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9973209.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(18403..20418)
                     /locus_tag="IWM52_000939"
     CDS             complement(18403..20418)
                     /locus_tag="IWM52_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9973210.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(20597..20902)
                     /locus_tag="IWM52_000940"
     CDS             complement(20597..20902)
                     /locus_tag="IWM52_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAP9973211.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(20991..22022)
                     /locus_tag="IWM52_000941"
     CDS             complement(20991..22022)
                     /locus_tag="IWM52_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP9973212.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            22229..23104
                     /locus_tag="IWM52_000942"
     CDS             22229..23104
                     /locus_tag="IWM52_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9973213.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(23256..24098)
                     /locus_tag="IWM52_000943"
     CDS             complement(23256..24098)
                     /locus_tag="IWM52_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9973214.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(24190..26067)
                     /locus_tag="IWM52_000944"
     CDS             complement(24190..26067)
                     /locus_tag="IWM52_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9973215.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(26064..27485)
                     /locus_tag="IWM52_000945"
     CDS             complement(26064..27485)
                     /locus_tag="IWM52_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9973216.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(27488..30877)
                     /locus_tag="IWM52_000946"
     CDS             complement(27488..30877)
                     /locus_tag="IWM52_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP9973217.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            31134..32525
                     /locus_tag="IWM52_000947"
     CDS             31134..32525
                     /locus_tag="IWM52_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9973218.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(32598..33245)
                     /locus_tag="IWM52_000948"
     CDS             complement(32598..33245)
                     /locus_tag="IWM52_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9973219.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(33253..33939)
                     /locus_tag="IWM52_000949"
     CDS             complement(33253..33939)
                     /locus_tag="IWM52_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9973220.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(33945..35018)
                     /locus_tag="IWM52_000950"
     CDS             complement(33945..35018)
                     /locus_tag="IWM52_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP9973221.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            35240..35914
                     /locus_tag="IWM52_000951"
     CDS             35240..35914
                     /locus_tag="IWM52_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9973222.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            35919..37259
                     /locus_tag="IWM52_000952"
     CDS             35919..37259
                     /locus_tag="IWM52_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9973223.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(37408..38394)
                     /locus_tag="IWM52_000953"
     CDS             complement(37408..38394)
                     /locus_tag="IWM52_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAP9973224.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            39118..40149
                     /locus_tag="IWM52_000954"
     CDS             39118..40149
                     /locus_tag="IWM52_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9973225.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            40258..>40681
                     /locus_tag="IWM52_000955"
     CDS             40258..>40681
                     /locus_tag="IWM52_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP9973226.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQI"
BASE COUNT        12636 a         7377 c         7648 g        13020 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt
      781 cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt
      841 atttcagcag aaaacaaaaa aattggcgca gctggcgtag ataagttgta ttaatggtga
      901 gactttccca tagatacaac tttttagagt gcatttctaa agcttgtatc tatgctagac
      961 atgaacattg actgccgcat agattagaat ctatgcggtt ttttgtgtaa agacaatgac
     1021 cgctttggcc attgtctttt tttgtagact ggatacttgc ctgattataa taaataaaaa
     1081 gattgacttt gcaggaggaa aaaaatgaaa aacaaacgat taataacagt tgtagtaatt
     1141 attttagttt atttagcagg tgcatttatt tatggaaaag cctcaggcga aaaagaaagt
     1201 acttcgacga accagacaaa aaaaacaaca gttgttgggg tattgcaata cgtgagccat
     1261 ccggcattag atgagattta tcgaggaatc aaagatggtc tgaaagaaga aggattaaaa
     1321 gaaggagaaa atcttgagat caaatttcaa aatggacaag ccgatcagag caagctagcc
     1381 acaatgagtc aacagctagt acaatctgat ccagatgcct tgattggaat cgcaacacct
     1441 gccgctcaat cattagcaaa cacgacttca tcgatcccag ttgtgttagg tgctgtaacg
     1501 gatcctgtgg gggctggatt agtcgcttct ttagagaaac ctggtggtaa tgtaacaggt
     1561 gtttctgatc agccgcctgt tacttcacaa atcaagcttg cttctgattt gttgccaaat
     1621 gcaaaaaaag tcggaatctt gtatgcttct tcagaggata attcaaaata ccaagtagca
     1681 caagcagaag aagctgcaaa aaaagaaggg ttagaatcag taaaatatgc ggtgccttcc
     1741 actaatgaga ttgcacaaac ggttcaagtg atgagccgac aagtcgattt tatctatgtt
     1801 ccattagata ataccattgc caatgcgatg caaacagtgg taaaagaagc aaacaaagca
     1861 aatatccccg tcattccatc tgtggataca atggtggaac aaggtggact agcaacaatc
     1921 gggatcaatc aatatcaatt agggcttcaa agcggtaaga tggcggcaga acttgcgagt
     1981 ggaaaagaaa aaccggaaac gacgcctgtt tatacatttg atcaagggga tacagtgatc
     2041 aatcaatctc aagctgatcg cttaggaatc attgttcccc aatcgatgaa ggaaaaagcc
     2101 aaaatcatta cagatgactc gcagcaagaa acatctaagg aggatgacaa atgatcgtat
     2161 cagctatttc acaaggcatg ctttgggcaa tattaggatt agggattttc atgacttatc
     2221 ggatcttaga ctttcctgat atgacgacag aagggtcctt cccattaggt gctgctgtct
     2281 gtgtgacagc tattactcat ggggtagcgc cgattgtagc tacattatta ggcgtaggtg
     2341 ctggcatgat agcaggactc gttacggggt tactttatac aaaaggaaag ataccggtta
     2401 ttttagcagg gattttagtg atgtcaggat tgaactcagt tattttgttt gtgatgcgaa
     2461 caccaaatct ttctttattg aatcatccga ttctacaaga cagcttccat cgctttgggt
     2521 tacctgatta ctttgacaca gtccttcttg gcttcatctc tttgggcatc ttgattgctg
     2581 tactgctatt cttcttcaat acagatttgg gtcaaggcta tattgcgaca ggagacaacg
     2641 aaacgatggc tcggtcgtta gggataaaaa ccgatcgtat gaaaatttta ggtttaactt
     2701 tgtcgaacgg tgtcattgca ttatccggtg cgctgattgc ccagaacgac ggatatgcag
     2761 atgtaaataa agggattggc gtaatcgtca tcggtcttgc ttccatcatc atcggtgaag
     2821 tgatcttcca agaattgacg ctagcagaaa gattgattgc tattgttgtc ggcagtatca
     2881 tttatcagct attgatttta gtagtaatca aactagggtt tgacacgact tatctgaagt
     2941 tgttctccgc tatcatctta gctatttgtc tgatgatccc gcaattgaag aaagcactga
     3001 acttaaaaac gggttttgag aaggaggcat agtaatgact gatatattga caatcaaaaa
     3061 tggcagaaaa gtcgtaaaca atggaatgaa tgaagagaaa gtactattgg atgaaattaa
     3121 ccttacaatc agagaagggg acttcatcac tgtccttggc gggaatggtg ctggaaaaag
     3181 cacactgttc aatacgatcg ctggtacgct tcaactcagt caaggatcga tctcttttaa
     3241 agatcagctg atcacaaaag attcagaaga aaaaagagca cgattccttt ctcgagtttt
     3301 ccaagacccc aagatgggaa cggctcctcg gatgacagta gcagaaaatt tgctacttgc
     3361 acaaaaaaga ggtcaaaaac ggaaattccg tttacgtaac ttgaaaaatc aaaaagaaga
     3421 ttttttcaga ctatgtcaag aagtcggtaa tggcctagag caacacttgg atactccagc
     3481 gggtgaatta tctggtggtc aacgccaagc attaagcttg ctgatggcga cgatcacagc
     3541 tcctgagtta ctattattag atgaacatac tgcagcttta gatcctaaaa cagctaaagc
     3601 attaatgcga ttaacaaatc agcggatcaa tgaacaaaaa ttgacttgtc tcatgatcac
     3661 gcatcgaatg gaagacgcct tgaaatacgg aaatcgtctg atcgtactcc aaaaagggaa
     3721 aatcgttcaa gatatttctg aaaaagaaaa gcaacatttg acaatgggcg accttttgca
     3781 gttctttgaa gaagtagtgg attgatttcg acagaaaaat tcctttcagt agaaggactt
     3841 gaaaactcat tgacatcttc ctttgattca tcctatactt aaaacaataa acaattaaac
     3901 atatttgggt gttttgtagg atcacgtagt caatgatttt aacaattaaa tagaatattc
     3961 tttgctctga caagtaaacg atgagaaaag aatttgctac tttgccacct agacgtttcg
     4021 tactttttcg tatcaaacgc taagtggctt tttttattga agtaagaaca aaggaggaat
     4081 aagatgattt ctggacaaac caaattagct gggctctttg ctgatccggc ttctcacagt
     4141 ttatccccat tgatgcacaa tactgctttt gaagcaaaca acatcgatgc ggtgtattta
     4201 gctttccaag taaatccaat gaatttgaag caagcagtcg aaagtattcg aacttttgat
     4261 atgttaggtg taaatctttc gatgccgaat aaaacagcgg ttattcctta tctggatgag
     4321 gtaagccagg aagcgcaatt gatcggagca gtgaatacga tcgtgcacga aaagggtcgt
     4381 ttgattggct ataatactga tggtgcgggc ttcatgcaat ccgtgcaaga agctgggatt
     4441 tcgatcaaaa agaaaaaaat gactctactg ggagctggag gtgcagcaaa ggcagtcact
     4501 gttcaagcag cgttagacgg cgctgaggaa atcgtagttt ataaacgaaa caaccaaaca
     4561 ttcgctcaag taaaagaaac attcgaacaa ctcagctatc gaacttcttg taagataaaa
     4621 gttcgtgact ttgccgatac gcatcaatta agaaaagact tgaaagaaag ctgtcttcta
     4681 gtcaatgcaa cagatatcgg gatgggaaag aaaatcaatc agtcaccgct cttagatata
     4741 agcatgatgc atgagaatct agcggttttt gatttgattt atgcacctag agaaacacgt
     4801 ttattaaaag aagcaaaaaa agtaggagca aaaggataca atggacttgg catgttgatc
     4861 taccaaggag caatcgcgtt tgaattatgg acaaaggaaa agatgccgat ccatgtgatt
     4921 gaaaagctgt ttgattaaaa aaggataata gataaagatc ttttattctc gcataaacgg
     4981 tctcaagaaa agtaatcctt tatgtatcga agcatttatt tcgttatttt ttattaaatt
     5041 atcaaaaaat gataggcttt tgtcgattgg ttatcgtatc ctttatttat cagtaagaaa
     5101 cgaggaggaa ccacaatgag tcggatggat cgctataagg acatccataa gaaggcgaaa
     5161 cctctgaagc gggatacggg gagtattttt gctagaagag aacgaaaacg aacgcgtatg
     5221 gaaaatgagg aagatgctac ccgtatttat gaaagagagc agcattcatt tgaacatttg
     5281 aaccaatacg agcaacagat atacagagat gcaccaagag cagaagaaca acaaaaaaat
     5341 aaaaaatttt tcaatcggca aaagaagaac aaaccgttaa aacctcgaag aaagcgatcg
     5401 tggaaaaaaa tcattctatc gattttgctt gttttgatcc tttattcagg tatctcattt
     5461 tttattggta aatatgtagc tgaacatgac acaagcttag aggcagcgac ttcagaaacc
     5521 tttcatgggc aaaaagcgac gaatggtgcg cataatattt tgatccttgg aagtgataca
     5581 cgcggagaag atgccgggcg agccgatacg atcatggtct tacagttgga tggtccttcc
     5641 cataagccga aactgatttc ttttatgcgt gacagcttcg ttacgatccc aggggtagga
     5701 caaaataaga tcaattcagc ttatgcatac ggaggagccg atctagtgcg tcaaacttta
     5761 gtagaaaatt ttggaatcga ttgtcaatac tatgcgaaag tcgatttcaa atcgtttgaa
     5821 aaagtgatcg atgccttatt catgaacgga gtgaagatcg atgcagaaaa agatctgaat
     5881 ctggatggtg tagatattaa aaaagggggc cagaagatgg atggacatgt tcttttgcag
     5941 tacgcacgtt tccgaatgga tgaacaagga gactttggcc gtgtcagacg ccaacagcaa
     6001 gtaatgaacg ctattttcag ccaattgaag aatccattga atctgatccg tgcaccttat
     6061 gcagctggta aagctttagg atatacatcc acagatgtca ctgcttggtt cttgacgaaa
     6121 aacctctttt cgattgcacg aggagctggt ggcattgatc gaataagtgt gccagtagac
     6181 ggttcgtgga attatgggca aagtgattat gctggcagta ttcttgaaat tgataatcaa
     6241 atgaatcaat ctgctattac agattttttg aataaatgat cataaaaaag atcaagaaga
     6301 agctgcgcaa ggcttttgtc atgaatcaat caaacgattc actcgtaaaa aacgctttct
     6361 tatggtagat ttaaccaaga gaggagctgt ttacattgga tatctatctg aacttcaaaa
     6421 atgagtcgcg tgaagccatc gctttttatg aaaaagtttt tcagactgaa gcatcaggta
     6481 tcaccacata cggtgaaatg cctggaatag aacaaatgaa cttgccagaa gaagcaaaaa
     6541 atctaatcac gaatgcacgg ctgaacattt atgatacatt agttatgttt gcagatacgc
     6601 cagaaggaat tggtcctgaa ttagttgtgg gaaataatgt ttctttagtc atccagacaa
     6661 gaaatgaaga agaaacgaag cgcctatttg aaagcttagc tgagggtggt aagattgaga
     6721 tgccactgga tgctgttccg tgggctcagt tgtatgggat ggtaactgat cgatttggca
     6781 tcaactggca gattaaccaa gagtgacagc taaagatgaa tattttctgg aaacatcaag
     6841 cagcaaacgg atcatcgttt gtttgcttga tgtttttgtg tctatttcga ctatacggta
     6901 tctttttttt attgccagat gcgaaaatat gttccataat agagaagtcc gcaaaaaatt
     6961 aagtaacgga ggtcagaaga tgaagacggt gatactagct gaaaaacctt cacaagcaaa
     7021 agcctatgcc gaaaccttcc aacagcaaaa gcggcatgaa gggtattatg aaatcagcga
     7081 tccattgttt gctggtgaag taaccatcac ttatggtttt ggacatttgg tagatatggt
     7141 tgctccagga gcttatgaag agcgttgggc aaaatggtct ttagaaaatt tgcctatttt
     7201 cccagaaacc ttccgttacg aagttccaat ggacaaacgt gcacaatttt cgatcgtaaa
     7261 acgagaattg cagtcagccg ataccatcat tattgcaaca gatggtgatc gagaaggtga
     7321 agccatcgcg tggtcgatta ttcgacaagc aaacgcattt tctaaggaaa agaactattt
     7381 acgtttatgg atcaattctc ttgaaaaaga agcaatctat gaaggtttta agaatctcca
     7441 acctggaaag aactattttc caaaatataa agaagcgcaa gcaaggcaga acgctgattg
     7501 gctgattgga atgaatggaa gtccattata tagcttgcta cttcaacgaa aagggattga
     7561 tggcagtttt tctcttggtc gtgtacaaac accgacactc tatatgattt atcagctgca
     7621 agaagaaatc aaacacttca aaaaagaaac gtactttgaa ggagaaggtt taattacgac
     7681 aaacaaaggg cagttttctg gaaaaatcgt accaaaaaaa gcatttaaaa cccaagaaga
     7741 gttaacgaag gaaatagaaa aacttggagc gcatttaggt aagcaaaacg gacggattct
     7801 tgaagttacg aaaaaggaaa agaggttgca tagtccacgt ttattttcgc tatctagttt
     7861 gcagacaaag atgaatcagc taatgaaagc aagtgccaaa gctacacttg atgctgctca
     7921 aggattgtac gaaagcaagt ttctaagcta tccaagaacg gattcctttt acattacaga
     7981 aaatgagcat cagtatctgg ttaaacatct gcaaaattat aaaaaatttt taggaataga
     8041 agaagtacag acaactgaga tcaaagcaaa gaaacgatat gtcgatgcga aaaaagttca
     8101 agaacatcat gcaattattc cgacaaagac gattccgact cctgcacgct ttgcgaagct
     8161 ttccaaatta cagcaagcaa tctatttgca agttttgcgc actactgtgg ctatgtttgc
     8221 tgcagattat atctacgaag agacgaccgt aatgacgggc gtacagcaat taaagctaaa
     8281 aagcatcgga aagatcccat tacagcaagg ttggcaggaa atcttacgca tcaagtcaaa
     8341 aaagaaagaa gctcagactc ttcctcctat cgtagaaaat gaagaagcag aagtagaact
     8401 gaaaagtatg gagaaagaaa cacaacctcc aaaaccctat accgaaggaa cgttgatcac
     8461 agcaatgaaa actgcgggaa aaacgctgga taatgatgaa gcgcaagaaa ttctcaaaga
     8521 agtagaagga atagggacag aagctacacg agccaatatc attgaaaatc ttaagcagaa
     8581 gcattacatc gaagtcaata aaaacgaaat aactgttacg ccaaaaggaa tcactctttg
     8641 taaggcagta gccaatgaac cattactgac gagcgcagaa atgactgcgc gctgggaagg
     8701 gtacttgaga aaaattggta aacaagaggg gacaccatct gtttttttag agaatatcaa
     8761 aaaattcatc ctacatttat tggcagaagt tcctaatcaa atcgacaagg tagacttttc
     8821 agatgagatt agcgggacac gtcagataaa agcgatggag aagaaaaacg atcaattagg
     8881 aagatgtcca aaatgtaaaa agggaatagt catgctttat cctaaaatcg ctacatgctt
     8941 gaatccggaa tgcgacttca aattgtggcc aacagtagct aaaaagaagc tgactaaaac
     9001 gaatctcaga gatttgcttt caaaaggaaa gacaagtaaa gttgtcaaag gttttactgg
     9061 taaaaaaggg aaattcgatg cagttttagt attaaaagaa gatatgacaa tcggtttttc
     9121 ttttccatgg atcgaaaaca gtggaacaaa agcagttgat ggaaacaaag aaacatcaag
     9181 aaaaaataat gaaaacgaag tagagtaaat gaaaacaatg aaatagcgga atatttattc
     9241 cgaaaagtga gaatataaat accaataaaa ctaaaaaata tcagtgaatc aagcaaaaac
     9301 gttggggtag gctgatttta tatagatagc aaggagtttt tttatttgaa aaaagtgcat
     9361 tcatttttaa attatcttat gggagcaatc acgattgtgg ctgtgtacca agcatttcta
     9421 aagtatcctg attacgggac gaagttgttg tcattgacgc acaatcaaac agcatattca
     9481 ccaacgttga ttttatcgac tttgactgca ggagctaaaa gcgctactta tttttttcat
     9541 aaaacgacaa gcatgctaaa gcgaaagaaa cagcgaaaac aattgttggt ggtggtagcg
     9601 gctatgatca ttatttattt tctaccagtt attacacaag tatttcatca atattttggt
     9661 taagaatacg agagtttgtg acaaaagtca tgtcacaaac ttttttcttt gtcatttttt
     9721 cgtaagaaaa agcaaaataa aagtatatac attgattaaa agtatatact tggatataat
     9781 gtacttacta tagaatgaga atggtataga gggtggcgaa atatgaaaaa aatgaaaaag
     9841 tataaaatta gaaaatctgg caatagcgat gtaacaacta tcccaccaga agtaaaagaa
     9901 ttcatgggtg tgcagacagg tgacgcaatc agttatgttt ttcaatctga tggttctgta
     9961 cgaatgatca aagcacaaga agaacctgat attgattcat tagttgattc gatcatgaat
    10021 caatatgaag acgcgttaaa agatttggtt gatttatgag gtatctttcg actaaagaaa
    10081 ttatcaaaat caacgcgaaa gttattttac gtcactcgcc aggcgaaatg atcggtgtaa
    10141 aagatgcaaa tgcattagat atggccgtca aacagccttc acaagcagta ttcaatcaag
    10201 aactctatcc tgaaatctat gaaaaagcgg cgattttagc aattaattta gctaaaaaac
    10261 atccttttca taatggcaat aaacgaactg ctcttgttgc catgttgttg tttcttcaat
    10321 tgaataattg tccagttgct ttttctcgtc aagaagctgt tgattttatc ataatgatta
    10381 ccactagttc attggatttc gattcactaa agtccaagat aaccagttat ttacgacaaa
    10441 ccgcaataaa atgaacgaat taaaaacttt atttctcaat tagatcagca tgtcattaag
    10501 gaatatgctg atttttttat gtttaatcat acaaaatagt tttttcataa aaatatggaa
    10561 aatatggatt aattttgaaa taattgattt aaaaggaata caaaagagtg aatagtagga
    10621 aatagtggtt tagaactagt gggctgattt tttatatatt ttagtttaca taatatatat
    10681 tatacaaagt agatttaaaa aatgataaac ctctctcgaa cggtttttta tcaaaaatcg
    10741 tcatttttcc atcaaaatct gcttatgcaa aattataaaa aaattttgca gtttttctta
    10801 ctttttttgt cgtttaattg aatgataaaa accagtttct ttattcagtt aatcaaatat
    10861 gaacgaatta gatatgaatc attgtatttg ccgttgcgga aggatttttt aattaaccac
    10921 agataaacat tgataacgat tcgcttattg taatcaacag attaaatttc atatgttcat
    10981 atgtacggat acattttcgg accgattaat tttaaccatt ctgtttattt gcttacctat
    11041 tagatatcaa taaaaaactg cttaacagct atttcagctg ttaagcagtt ttaggatttt
    11101 tagttcatat tcattgtttc ttataataca gtgttcaata cttctcttaa agcacttgtt
    11161 gatttgctga acattgcttt ttcttcttcg ttgattgata attcaacaac ttcacgaaca
    11221 ccattttcgt caacgattga tggcacacct gtaaagatat ctttttcgtc gtattcacca
    11281 gttaaataag cagatacagg caatacagct tgttcgttat tcaagatagc ttttacgata
    11341 cgtgcagtac tcataccaat accatagtaa gttgcttttt tacgatcaat gatctcataa
    11401 gcagcattac ggactttatc agcaatcaca tctaattcat cttgggcaat acgatggtct
    11461 ttttctacaa tttcaaacac tggtttacca ccgacagttg tatgtgacca tgcagcaact
    11521 tctgaatcac catgttcacc taagatatag ccgtggacgc tgcgtgggtc aacttttaat
    11581 ttcaatgcga tttctttacg gaaacgagtt gtatccaatg ttgttccagt accgataaca
    11641 cgtgatgttg gcagaccaga ttcgttccaa gcgatatatg tcaaaatatc tactgggttt
    11701 gaagcaacaa cgataatacc gtcgaatcct gatcccatga tttcttttac gatttggcgc
    11761 atgattgacg catttgtttt aactaaatct aaacgagatt gaccaggttt ttggttgata
    11821 ccagctgtga taacaacgat atcagcatct ttacattctt catagccacc tgaccataca
    11881 gccacatttt catcgcccca agccatacca tctaaaaggt ccaatgcttc cccttctgct
    11941 ttttcttgat tcacatcgat caatactaat tcattagaga ttccttggtt gatcattgca
    12001 taagcgatac ttgttccaac aaaacctgta ccgacgatta ctactttacg acttgttttt
    12061 ttcatgaata aacagctcct tttcataact atttgttact ttcatgaaaa gtatagtctt
    12121 gtttattcat tttatcaaca tgttaattat aatagttgtt attataattt ttgatggaaa
    12181 cgaattcctg aatacgcata acagataaat gacaaatgtt tacacctata tcccctgata
    12241 atacaaccaa aagtttagaa ctgaaaataa atccaaaaaa attcgctgca acgatataat
    12301 aagacagcaa tcgcacctaa aacaattttc cttcatatgc tttagtgaaa gattttcttc
    12361 ttaatgttta tttctccatt cattgggagt acatgttgaa tacttcttga ataatttgaa
    12421 taaagaagtc gtttgtttat atccgatacg attagcgatt tcttccaacg aaaaactcgt
    12481 ttcttgaatc agttccaacg caaagtcgaa ttttttcttt agaaccagtt cttgaaagga
    12541 agcatcaaaa tacattttca atttattact aagatagacc ggattgtagc caaaatgagc
    12601 tgctgtttct tttaacgtac aagaagtaaa atgcttatcg atataatcta atactctaag
    12661 catctcattt tctgcgcatc catctgctga tgaaaagctt tgttgttcta ataaatgagt
    12721 caattcgatc agtaatagtg atgtatagag atttaacgaa cggatcttat gactgtatgt
    12781 atcaaaataa atcaacagca tattttcaat caaatgcgtc aaacgacttg tttttccgcc
    12841 gcgaaaatga atgaattgtg tatgttcccc tttttctttt gatgcattta tcaaaaaaga
    12901 agaaagtaga ttatttgtgc ttgccaaatg attcaatatc tctgtagata aggtttcttc
    12961 ttttaataag atgttgatta aaatatcctc ttgccctgtg tattcgattc tttgaggtgt
    13021 ttactgatat gaatatttcc ctgttgaaat agatcccctc ggtggatttt tggcggtgtc
    13081 tgaagatggt aagctaagtt taaatcattg atattcattc cttgttcacg ctgcagagtt
    13141 tcgattgatt cttcgttacg caaaaaaaat gtctaattca gctagttcca attgactccc
    13201 tcctttttgt aagcggtttc tttcgcttat tagttcacgc tgtactgcta ttatatgcaa
    13261 agctattttt ggtttcataa agactttcta tggttgggac atgaacttcc tgtgattccg
    13321 ctattgagag aataaccact tcatactata tttttgataa gcgcttacaa aaatgaaagg
    13381 aacatgctta tgaataaaaa gatgatgaaa ttttctctgc tgtcagtctc tcttattttg
    13441 acttctgctg gtgcagtcac gtctgcaatt cccagtatgt cagctactta tgccacgcgt
    13501 tctttatctg ctgttgaatt attaacgact gctccctctt ttatggtcac tatttttgtt
    13561 ttgattagta gttttattgc taggaaaatc ggacaaaaac aaactgtgat catcggatta
    13621 gtgattgcat cgatttgtgg tatcctcccc ctcttctcac aaagttacag tattgtctta
    13681 gcttcaagag ctggactagg tattggcttt ggattgatca attctttggc agtcagcatg
    13741 attgctgcct tttatcacgg agatgaacga gcctctatga ttggttttca aagtgcattt
    13801 caaggatttg gtgcagcttt aatgacattt gttgcaggac agctagtaca gttcggttgg
    13861 caatatacct ttttagtcta cgccatcagc ttacctattc ttgtcttgtt tgttttgttt
    13921 gttcctacac ctgaaacaac tgaacttcaa tcgcatggca cagccccaac aagacaagta
    13981 atcaaaacga atcctaaaat gattagttat ggtatttttc tcttgctggt catcgtggta
    14041 tataatgctg tatcgattaa attagccaca gtcctgacga cttattctct aggaaacgaa
    14101 actaatgcag cgaatatctt aacaatcatg caattggcaa gtatggctgc cggagtttgt
    14161 tttggctggc ttcagacaaa agcacatcgc tatatgctta cactttcttt actactgatg
    14221 ggaatcggct ttgtattgat tgccactgtg ataaacctgt atgtgattgg gtttggtgcg
    14281 atcctaacag gaatttcttt ttcgttattc attccttact tattcaacca agtaagtatc
    14341 caagcgccca agtacgcgga gagtttcaat acttcaatct tattggttgg cgctaattta
    14401 ggtactttca ttgcaccata tggtttgctc tttctctctt ttgcttctct tgggagtaaa
    14461 acacttgctg ttttcgtcaa tggcagtatc ttgctgttga ttagcgcgct tattagcctt
    14521 ctagtagtac acaggacgaa tagaaagaca gtgataagag tagatgaaga aataaaagaa
    14581 caattgacaa caaaataaat ttgatggagg tcgtgtaaaa tgacaaatga aaaacataaa
    14641 gatagatggc tttggtatcc tggagacttt gagatccgcc atggactgct acaaaatttc
    14701 caacgggaag aacgaggttt tgactggcca gcgtactggt acatggatga ttgccatcgg
    14761 aacgtcaagt ttaagagata ttactttctt gatcaacctt ccatgtttaa agtgacgatc
    14821 caaggagttg ggtatgtaga aattaatgga caaaaacatc catgtggaaa atggctgact
    14881 tgtccagctg gtaaagcaaa aattcgtatc tttgtaggac atacatcagg gctaccagcg
    14941 atgtttatcg agggagacga agtaaaatca gatattggct ggacagcaag taattttatt
    15001 gaagaatatc cagctggctg gagtccgctt tatgtcgata tcgcaaaaga tccaaatcaa
    15061 atctattatc aaaaagaatg cgtccatcca gtcactgaaa cggagattgg cggaggaatc
    15121 ttattcgatt ttgggcgagc agtgaatggc tgtgtagaac taatatctct ggatgaccat
    15181 atcgaatcta tcactctatg ttatggagag tccgatgtag aagctatgga tatcgattat
    15241 tgctactata agcaaatagg aatgacaaga aaaatgaaag ctcgtaaacg cgcttttcgc
    15301 tatttgttta taccagaggt ttctactgga atggttcaac ttcaagtttt tcatgaattt
    15361 ttatctcttt cgaaacaagc tgctttttca agcgattctt ctttactgaa tcagatctgg
    15421 tcagtatcag aagaagcctt caagttatgt agtgatttgt tttttatcga cggtatcaaa
    15481 cgagatcgct ggatttggtc tggagatgcg tatcaatcat tcatgatcaa tcaatatctt
    15541 ttttttgacg aggaaatcaa taaacgaacg atattagcat taagaggaca aaatgaaatc
    15601 aagcagcata tgaatactat cgttgactat tcgatcttgt ggttgattgg tatcgaaaac
    15661 cactatatga tgacgaaaga ctatgaattt ttacgcatcg tctaccctaa gatgcaatca
    15721 ttaatgagat acttgatgga gcaaacgaat gaattagggt ttatttatgg gagagcaaaa
    15781 gattggatat ttattgattg gtctgaaatg gataaagaag gaacgcttgc tgcagaacaa
    15841 gtcttattac tgaaagccta tcattccatg atcacttgcg gagatatctt agctgaaaca
    15901 tatggagaga acgaaaatta tcatactcat ctctatcaag aaaaatatca actattgaaa
    15961 caaaacctat ttgactactt ttgggatgaa gaacttggcg cttttatcga ttgctatgaa
    16021 tctgggagaa gaaatgtgac gcgtcatgcg aatattttcg ctgttttatt cgattttgct
    16081 aacaaagacc aacagcaatc gatcttaaag aatgtcttat tgaatgacac tatcacacag
    16141 attactactc catatttcaa attctatgaa caagatgctt tatgcaaact cggacagacg
    16201 aatatcgtct accaaacgat tcttgattat tggggaggga tgttatctca tgatgcggta
    16261 actttctggg aagaatacga ccctactcaa acaggaaatg aaaagtacag tatgtatgga
    16321 gatccttatg gaaaaagctt atgccatgca tggggagcaa gcccaatcta tcttttagga
    16381 cgctatttta tcggtttgta tccaacaaag ccaggttatg aagaatttat gatacaacct
    16441 aatctagatt catttgaaac actgaattgt acgctaccta tcaaagatgg aaagatttcg
    16501 cttgaaaaaa caaaagataa gatcgtgatt acttctgata gaacaggcgg aacagtgaaa
    16561 atgaatggta aaagttatcc tcttctagca aaacagccac ttgtcttaac aagctattca
    16621 aaaataaatg cataaaacaa aaaactgctt gcgacatatc agaagacttt aaatcttctt
    16681 ctgatattgt ccaagcaatt tttattttga agtttgctta attctgttct ttagaaacaa
    16741 tgtttgttct ttttccggca aaaagaaagc tagataatac gacaagtaaa aagaaagcga
    16801 tgattccaac tgtcaaggct atttgaccgt attctcctag tttcccaagt acaagtcctg
    16861 gtactagata atcagtatcg gcaaatgtag aagaagcgat tttaagatct cctaatactg
    16921 gaaggataaa taatggcagc caactaatca ataagctatt gaaagcagaa cctaataccg
    16981 cccctcttct accgccagtc gagttgccga agacaccaga agcagctccg cagaagaaat
    17041 gtcctactac cccaggaatg ataactgtag tttgcaagcc tatcatagca aacatggata
    17101 gtgttcctac aaagaagcta acaaaaaagc caattaatac agcattaggt gcatatggaa
    17161 aaacgatagg aacatctaat gctggtttag aatcgggtac gagtttttta gcaatccctt
    17221 ggaatgctgg aacgatttct gccaacacca ttcgaacccc ttgaaggacg ataacaaacc
    17281 cagctgtgaa tgttccagct tggatcagtg caaagattac agcgttgtct ccattgctta
    17341 gttctctttc aacaaatcca ggaccagcta acaaagcaag tgcgacatat acgatcgaca
    17401 tcaagagcat gatcgataca gtagaatctc gcaaaaagct gaaactttta ggaatcttta
    17461 tgtcctctgt agaacggtct ttgtttccaa aaaactttcc gatccatcca ctagaagcat
    17521 aaccgatatt tccggtatgc cctaacgcaa cttgattgtt tccagtgatg ttcttcataa
    17581 acggttgaca aagagcaggc gtaatagtta atagtgttcc ttcgaagacc ccgcctaata
    17641 agatcatcca caaatttgca gtaaaccccg cgcttactaa aatgactgct gttagacaag
    17701 aaacataaag cattgcttga cctgtaagaa aaatgaattt atatcttgtg aatctagcaa
    17761 gtaaaatatt cacgatcatc cctactagca tgatcaaagc agtaggtgta ccgtattgtt
    17821 gcaaagcaat cgccactact gcttcattgc taggaactac tccttgagta tgcagagctg
    17881 tttggaacat cgaagcaaaa ggggttagtg aattactgat taaaccagca ccgccgatca
    17941 gtactaggaa tccgacaaat gttttgatag ttccttggat cacttctgaa aatttcttct
    18001 tctgaagaac gagaccaagc agtgcaatca aagaaacgag gatggagggc tgactgacga
    18061 ttgacacaaa caaattcaac aatttatcca ttgatagttc ctcccttgaa tcatttttat
    18121 tcttttgaca agatcttcgt tactttttct tttacttctt cgctgtcaat gatcgattgc
    18181 aaaggtacga gcttttcatc aggtatagaa gttagcgtat ctttcaaaga atgctcaatg
    18241 aagaaataat cagccatatc gctagataca cttccaacat ccgtatgact tacttcaaac
    18301 tcgtccacat ctgctccaag atctgcaagt gcttgtttga tgttcatctc taccataaaa
    18361 cttgttccta atccactttg acatactgca aaaattttca tatcattcgt ctccttttct
    18421 tatgatcgct aatatttctt cttttgttga tgcgtgtaat aaatcttgaa gattttgttt
    18481 attagaaagt agctttgtta aatttgccaa agcccgaaga tgtgcctcat tatcgatagc
    18541 agctaaacag atgaacaaac taatgggatg ggctggatta tcaagcagat ccacaggttg
    18601 ttgaagtttc agcaaagaca tccctacttc gtttacgcca tcttctggac gagcatgagg
    18661 taatgcgatg tgatcgccaa tatggataaa cgcaccatac tgctccacgc gctcgatcat
    18721 agctttgata taactttctt ctattttatt ttctgataat agcggctgtg cggcaaatgt
    18781 gattgcttct tgccagtttt ttggttgatc cgataattgg atcatttctt ctgttaataa
    18841 ttcagatagc ataggacgtt gatcctcctt cttttctaaa agtttgttca ttttacgatt
    18901 taatacttga tagagctttt cttcgtctac gccttctttt aattcgatat aaggcagtag
    18961 cgccgacaac aattcttggg cagaaggcat tgaaaatcct ggtatcagcc aatctctttg
    19021 tacttgattg atcagctgat ttttttccaa gtcagtcatg attggatgca gaacatagag
    19081 agactttttc ttatcagtaa gtgtgagagg tacagtagaa aaaataatat cgtaagaagt
    19141 ttcgtcaacc tcgtgtaagc ggtcagctga attagtctct gtaaaaaaga tcatcgggaa
    19201 taattgctga agttcacttt ttaagatcaa tgaagagcta atgccacttg gacagacgat
    19261 cacggctctt atttttctat ttctttcttc tgcgtccgct tttctgatct ctccgccaaa
    19321 tagtatagtg aaaaaaccaa tctcttcttc agggatgctc ttccctattc gtctttctaa
    19381 cggaagtaat gcttttcttg tcatttcgta gatggaagca tattgatgct taatttgatc
    19441 gatcatcaca ttaggtaaat aaaaagaata ggaaatacga aaataggcag ggaccaagtg
    19501 ataaaagaca ttcatcaata attcacgaaa cgaagcaaat tctacggcca tcaggaattc
    19561 catgcgatgg atgacttctg aagaacattc cagcaaaaaa tcaagtgcag gctcctggac
    19621 atctccttca acgatcgatg ctaagcaaag agagaaaaat ataatctccg aattttgatg
    19681 ttcggaatag ccaaggccag tcaatacttg tcgactaatg tcagtgactg caagagaagc
    19741 caacagttca gcatctttta ccagttccat ggatgttttt ccaattcgaa tggttgtcaa
    19801 actcaaataa taagctaatt catccattcg atttggtgcg aatcgaagat ctgatagtga
    19861 aatcgtttta tctagcaaat ctctaacttt ggcgtaataa tcaatggaaa gggttgcggt
    19921 taaatacgtg agcccaaatc ttccagcaag tgactgcatc aaacgagcag tgcgattttt
    19981 tgcttctctt cttaatattg cttctgaccc tagcagccta aaaccagttt tcctagtata
    20041 cgacagctga caatcttcca cttctaattc atttcttatc tttttgatat ctgacaggac
    20101 tgtcccttta gaaacctgaa gaaaatcttg aaaatgaaaa acagaaacag acgttgcagt
    20161 cgaataaacc atcaaataaa gaagatctct tcgctcttgt tcggtataca cagcttcaaa
    20221 tcgagtaata tccaaaaatt tttctgccca gtcgattgct cggaatttct ttatacggat
    20281 acgggtatca tccacatatt cggcaaaagg agttaattgt ttgatcgcct cgcttgtatt
    20341 tacttcgatg atctctgctg ctttttctag actgatttct cttggtgttt tcaggagata
    20401 actaattttg tagttcacag cgctgttcac ctcctactta tattctagta tggtagcgct
    20461 tttaaatcga ggggtgtttg gtacaactta cagttggatt ttttttacga ctgtaccgaa
    20521 cgatttctat cgaatggata cagaaaaaaa gcaggtgacc aaatgtctcc tgcttttatg
    20581 aattgatttc gtccgatcaa tgatgatcat gtttattttt tgaggtcagt tcttcaaatt
    20641 tttctttgac atcttcaacc gctttttcga tatctttttc aaagtgattg ggctcattag
    20701 gctttgtttc ctcttctaca gaatttgaac cagataaagc tgtttcttta ttttctgttt
    20761 caccagagtt tgtgccagct aatgattctt ctttgtcttc tgtttcacca gaattagttc
    20821 ctgctaaagc cacttcttct tctgttttag gggccattga acctcttaat cgtttgtgtt
    20881 tttcaccgtc ataatttgtc atgttggttc ctcctagtag ttctatactt tttcatcgta
    20941 caataagaca gtttatgcgt caaaaaacaa acccgattct tacaaatatg tcatattttt
    21001 ccttctgatt cttgaatgct tttgattcct tcttccaaaa ttttcatttg taagattgtt
    21061 tcttcatctt tgatgacttt ttctgcgcca ttaactaatg tctcgtaaat cccttcgtag
    21121 acacggctgt aatcaccaac ctcggaaatt actttttctt catgataact cccttgttca
    21181 tctatatagg tcaatacacc ataatgttcg ggcaaatcga taccaaagtc ttcatgatca
    21241 ggcatgtaaa acattttcag atgttcttct tgtcgatctt ttgtttgttt cacgaacatc
    21301 cctttttttc cgtaaacgac gaaacttgga cgttctttga tgcggaaata actggatttt
    21361 atcgaaattt tcatatttcc atagaaaaaa tccagatcga agtaatcatt cattcgtcct
    21421 ttccctaaaa gttgacgtac atcatagctg atatcatctg gttcgccgaa ataagaaaga
    21481 acttgatcta ctgtatgaca agcatgtcca tataaatagc tgttcgcagt agaaaattca
    21541 tgcgtatttt ctggtacttc cggtctgaaa taatcataat gcatttctac ttctaaaata
    21601 tcccctaatt ttccgctttc gattactttt tggactgtca agaagtcaga atcgaaccgt
    21661 ctgttttggt aacattggat aaatagacct ttttcttttg ctaatgcaaa taactcttct
    21721 gcttcctttg acgtttctgc aaaaggtttc tctactagta cattcttacc atgttcgagt
    21781 gccatttttc catattcata atgaaattga ctaggtgtag tgatgacaac taattggatc
    21841 tcaggatcat cccaaagatc agacagctga tcagtatatt cgatgtcttc gaatgttttc
    21901 cacactggtt cagatgggga aaaaattttt ttgatattga ttttgttcgt aagttttctt
    21961 gaaaaaggca aatgatagcg atttgtactc tttccattgc caagataagc gattgtaagc
    22021 atgagtggtg cctcctttta ttgatgtttt cagtatatcc tagaaaagcg cagaaagtat
    22081 ttataatagc tattctggta aaaaagttca gaaaaacact tgttatcatt cggtttggtg
    22141 aaattaatgt tcaaagtaag gataaaaatc aaaaagatag tagcggaacc aagtgtttat
    22201 cagtaagata gaatttggag gtcagatcat gcttattgaa gaaaaactaa caaaacaaga
    22261 gttgtttacc acgacagaga aacggattgt cgattacatc cgtaggaata tcgaagctgc
    22321 agtctacatg acgattgaag aattagcaaa agctacgtat acttcccatt cagctatcat
    22381 tcgtttatgc aaaaaaatgg gattcagcgg atttaaagaa ttccgtttcg agctggggcg
    22441 ggaagttcat caattgattg ctcagttcaa tcaaacgatt gatcctaatt ttcctttttc
    22501 aagtgaagat agcactcagc aaattgcaaa gaaaatggcg gatttatcga tccagtcaat
    22561 aaaaaaagca caattgcaga ttgaacatga agatttagat caaatcggta aagtattgaa
    22621 caaggcacag cgaatctttc tttttgcaaa aggtgattct caaatcacgg caaggaaatt
    22681 ccagaataaa atggtcaagt tgaataagtt tttgatcatg gctgaagaat acagtgattc
    22741 ttcttggaat gcagccaatt tgactagcga ggattgcgcc atttttatta gctacagcgg
    22801 tcgcatccat cattatgaac gaataatgac ctatttgaag catgtagagg cccctacctt
    22861 gctgattacc ggaaatcagc attcagaaat ggccaaacag gctgatatgt gtcttgttat
    22921 atcgcaggag gaatatgatt ttgctaaagt agcgactttc tcctctcaaa ttgccttcga
    22981 ttacgtatta aatacgttat tctcagtgat atattctcaa aattttgaag agaatatccg
    23041 caacttgaaa aacaaacaag aattgatgca aaagggcctt cttaaagaat atcggaaaac
    23101 ttaagaaaag atcctcattt agtaataaga aagatagtgt ttgataggct ctctttgata
    23161 tcatcataaa aacaggctga agttttcttt ttcaaacctc agcctgtttg tttacggaac
    23221 gattcgaata gatatattat agaaatgagg aatagctaac ttctgtttct tattttgaac
    23281 gattgacgaa aaagatatat aagaaggagg aaaatcagta tacatgcagc taacaatcca
    23341 tacaggaaaa atcgtttgtt ctcggatact ttctgtactt ctttttgaac ttcttttgca
    23401 tgataaggaa cccggtgccc tctgacaagt aagcgatgac tgttgaccat ataaggtgta
    23461 caagttagga gtgtcacgaa atcctgccct ttttcgattc tcagatcttt tgtatcagtc
    23521 ggttctaccg tcttgatctg atcgacttga taagcgagtg tccgtccatt gatttcgata
    23581 tagaaagaat cacctaaagt cagtttcggt aagtctgtga acaactttgc ttgaggcagt
    23641 cctcgatggc tggaaataac ggcatgtgta ttttctcccc ccgtaggata agacgttcct
    23701 tccaataatg aggcaccttt ttctagcagc acagtattcg tttcatcaaa aattggcaaa
    23761 tggacattga tctcagggat cgtcaaaata ccgatcgtat gtttctcaaa ataggttcta
    23821 tcctttttcg caggttcttt tttcttagta aagggatctg cacctggatt tccaccttct
    23881 tttgctaatt gctggttttt ctttgtcatg ttttcttgga ttttagccat gacttcttca
    23941 ttttctttga ccgcttgttg ctgataatga gagatgatct gctgatccag ataattattc
    24001 agcgcgtcgc taacaaatgg ataagctagt atccctattc ctgagagaag caagaaaagc
    24061 atcagcagat ctagaagttt ccttttcttt ggtttcattt gtgttctcct tgtgatgact
    24121 gtttgataat aaaagaaaaa ggagcatttg cgtaatccgc tgctcctttt tccaattctt
    24181 gcaaaaagac taaatctgac tgtgcttgcg tctagcaaag tacagtccag caatcagtag
    24241 aaggactact cctgcaccga tatacacata gattccctta ccgcctgttg aaggaagtgt
    24301 acctttgtgt ttatttggta ttttttcagg agaaatcaac tgtcctgctg ttacataaga
    24361 ttgttcatcg atagtaaatg ctacacggtt tgttaatgga acatattttt ctggcgcttt
    24421 cgtttcttcc agatagtacg tgccatattt tagacctgtc acatcgatta aaccgttact
    24481 tgtggttgta aaaaccgttg ctgattcttt cgccgatacc cagctgacgg cttttgtgga
    24541 tgggtcgatc gataaatatt tcgctgtgtc actatcttga tcacgaacga cgaattctgc
    24601 tccagcaagt gtttggtctg atgtaacgtc accatctact ttaacaaatc gtttgccccc
    24661 agtaacgaca tcgactgacg gtggtgtttg atcatttgta tgcccgttat cgacatttgc
    24721 ttggttagaa aatcctttgg ttggatctgc tttttcgttc aaatgcatat agtaaacgaa
    24781 tttcaatgta ccgccaggag ttaatgaagg aatataattc ggatcaactg aaaccgtgaa
    24841 tccgtctgtt tgctcagtga cagaataatt tactgggtcg atttctttat ttccatcata
    24901 taaggcataa gcatacgttc ctgaagaatc attatcaaat gttaaagcag cgtcatgagt
    24961 atcgataagt ttgaatgttg tgtacttgtt ttgcgttcct tctttatcag cgatacctaa
    25021 tgggatattg acagaaatct catattgaat tttttcaccg attgcgacat cttttccatt
    25081 caattgaggt gttgttttat caactttaga cgtatcattt ttgatggtct tttctactgg
    25141 tgtttggcta tttgccagga tctcaaaagg cgtgattgtt tgcttttcaa tcatttccgc
    25201 attatcagga gcttttactt cttctaaatt atattttcca acttctaaat gattaacgat
    25261 caactggcct ttttcaatag atgcctcggc aaagtcattg ttgccgatgt cataagaatg
    25321 accagtaatg aaatgtttgg ctttggtttg atcagttgtc caagtgtaca atccatctgt
    25381 gacactttgg atgtattttt tgacgcttgg tgttccttct tctttagaaa taataaattc
    25441 tgctccattt agtgcttcgt tttcggcagt accgattttt gtaactttca acgttccatc
    25501 attaccgact gtatttttag ggtagagatg gatagtatct agttcttctg tcccgtattt
    25561 ataagagcca tctgcttgtt tgatcatctc atatacaggg aaagctaaaa ccatgtttgc
    25621 ggcagctgac actccgtctt ttggttcttc tttgatcgtg tagactgcat ctttcccatt
    25681 ttgtttttta ggtaaagata aagtgacatt tccatcagca tctgtcgttc ctgaagcaac
    25741 tggtgtacca ggagtcaaag actggactgc ttgttttgct gcatccacgg acgctccttt
    25801 atctcgttgc gcataaaatt cttgagtgac gttataaact gaaaatgaaa tatcggctaa
    25861 tccttggtat tgatcgaatt cgctcatttc tttcccgctg ttttggatta aaggatcggg
    25921 taaatcagtc attttctttt tatgaaggat gacttgagct gctgtctcct ctgcaaacac
    25981 tttttttgcg ccgaagctgt ttgtgagtaa tggtaaaata aggaaaagga ctcctaacat
    26041 aacgtttatt tttttatgat ttttcatttt ttcactcctg ttcttttttt gataaacaac
    26101 cagattgcaa agcttgcgaa taccatacca acaacgccta aaagtgtatt tttcgtttcg
    26161 tttgtttttg gaagaaatgg acggctattt tcttttttat tgactacttt caatatactg
    26221 tccgcctctt tatcctcaga aagaaaagtt gaaatggtaa acggaatttc tgtttcactt
    26281 aaaatataac cagaaggtgc ttcgatctct tttaatcgat aacttcctgt tttcaacccg
    26341 gaaattgaaa aagcgccatt tttatcggaa atcaggtgaa gcgcatctga ttcgttcttt
    26401 gtccacttgt atccgttggc tgtttgctgg aggtaatttc ctgctttgtt tttaataagg
    26461 aatactgcgc cagctaatcg tttatctggt ttatttgtct cgattttgac aaattgctta
    26521 ctccctgtat atacagagac tttctttgta ctgacaccaa aaccaaaatc cagtgttccg
    26581 ttgttgttga tttccttgtt cgcctgtgct gtactgctta gctgcatctg ataagaaatg
    26641 gtcagttttt ttccagcata tttttccaac gaagggatcg aaaataaaac atcaaatccg
    26701 tgaggtttct tgtgtatgac aaagccttct gtcagctttt ctccattcga tgaaatcgtt
    26761 aaactattag gtaaaaacgt taatgcttca tccgcactat ctgacaattc gaacttttga
    26821 tagtcaagga tatttacagg aattgtcgta tgcaaggaat aagtgatttt ttcaccaatc
    26881 gtaaaatcat ttctaggctc ttctaatacc ttctcaaaag gtggatcagg gtagtcgttt
    26941 tcttcatttt taggatacaa atggatagtt gaaagttttt gattgttttg tccatgtaca
    27001 ggaagaacaa ctaccatatt ctctgccttt tctttgacga cttctggtac tttggattca
    27061 atgaaaagat acgctttatc tcttttttct tgatctttag cggccagaga aaaagaagcg
    27121 ataccatctt cgttgttaag agttgtagtt gttgcttctg cggtaaacat accggaagac
    27181 gcaccgtttt ttgcgatctc tgcttgtgct tcttctaccg ttttgccctt ttcccgtaga
    27241 tggtaaaaag aatctgtgac atcataaact tggaatgtga caccatttaa tcctcgatac
    27301 gtttgtaata aagctttttc ttgtccgtca tttggatgat ttttcggcag ttgcccgttt
    27361 gggaaaagca atttgtgtaa agtaatctga accatttctg tttctgtttc tgttgctacc
    27421 tgagtaaaag ctggtttaaa tagtaacaag aagagacaca tactaagcca accaagtttt
    27481 ttcatattta gctgccccct ctttcttttt tcaaaaaaag ataccaaata gaaaacgcac
    27541 aaccaatcat ccctactaga tagattccta aacggccaat tcctcccgtt tcaggtaatg
    27601 gtacttttgc ctgattcgtg atgtctaaag aaatctggtt gtttttggct cctggtgaca
    27661 gaacagattc atgatcttgt ccatccactt gaattgaccc atcttcgtgt ataactatag
    27721 taactggctc ttttagacct tggtatcctt ctggtgtaaa agtttcggtc agcgtataag
    27781 ttccaggttt taaattttcg aatagaaagg tatctgtttc ttgtccatct tttggcgatt
    27841 ctaagtccat ttctggtccc tgcagtctga attttgctcc ttttaatgtc tgtccgttgt
    27901 cttctttttt gtgtaccgtt agatcaaacg cttttagtcg attttgatga gtgagcgtcc
    27961 atacattatt tgtcatctcg gtattttttc ccgtgtaaat gatttttcca tatttatcga
    28021 tttggaattc ataatttccc ggcgaagtat catatccggc aggaccagct gtttcaacga
    28081 ctcgatattt accaggtgtg agtgaatcaa aactggcaag acctttttca ttcgttgttt
    28141 ggctgtcaat tggctgataa gtaccatttg cttctttttt ctgtagggaa aatgctgctc
    28201 ctttaagatt tatctctttg tccgtccctt gcatcgcata cttacgtact gctacaggaa
    28261 cttcaacaaa aggattttca atagtcaact ggatcgtatc atcggaagta gtgactgttt
    28321 ttccatcaat ggttaccgta ccatcagaag cgatcttgat ttcccaagta gtctttttgc
    28381 ttaatccatg cccttctgga gcttttgttt ctgtcagcgt ataggtcatt tctttttgca
    28441 atttgacatt ttctggaaga gaataggttc cgtcgccatg atctgttagt aaagtatcaa
    28501 tagaatctcc tgttaatttg aaaacagcgc caataagatc cttttcacct gtagaggaat
    28561 ttttcgttat tttcaagttt aacggtgtga attgtttagt atttttccat gtcattgcat
    28621 ctatttgttg agaatcgtat cctggtacag gtaattcttt gattgtttgg taactgaatg
    28681 cttgaccttg attattgtat tgaggtagtg ataatatctc ttgataactt tctccgctat
    28741 ttgcagataa tttgtcaatg tctgcacgtt cccacgtatt tgttgtatct ttagctggtt
    28801 taatgattcg aatatatccg tttttccaag ctgcagcatt tgtcgtatgt tcccgttgaa
    28861 tctcaaaagt aacttgatct ggacgatcag ctagattgtt gtcaaattct tcccataact
    28921 ttttgatgtg aagactgact ccgggagctt tagcagatgg tataccgaat tcagctaatt
    28981 cattggtatc aatgtttggc tggaaagttg tcctgccgtt catttgatac cagaaatttg
    29041 gatggaagtc ctcattttct gtttggattc tcacttggta atggatttgg atttcttggt
    29101 cttttcctaa atagatctga ttgctcttga tggtatttcc ttctatggaa atagatggag
    29161 tgaccgttgt aggacttgtc cccacactct tgactgaaag tgtaccaggc tgataaatga
    29221 atggttctgc gattggatca tttatttgtc cattgcttac agttgctgag atctgtacag
    29281 cttttttggc taggtattca gagatatctg ttgcatgatc agctgattca tagtaaagat
    29341 cgcctttttc atctgatgaa accatttgtc gcatacgaga ctctacttct gcttttgaga
    29401 gaccagctgc cggatcgctt tgaagttgga tgccaagacc atgtatttcg attcctcgtt
    29461 ctttgagtgc catcgcttct ccgatggttg cgataaacgt actgtcgatt cttctggata
    29521 gattgttttg gtcaggtgca tcatagattc ttgagattag agaagtattt cccggccgat
    29581 cttgcgtatt agaaaactga gttccgtaat aattgctgct tgattgtgcg tgtacccgct
    29641 gtactttata ggaaaatgtt ggtacaccat ccgtcagcaa aacgatcact tttttatgtc
    29701 catttggaac ggatagcatg cttcctgcat ctcttagtgc tttttgtgta aaagtaccac
    29761 catttgtccg tgaaggtgta atggatttta cttgattttt cactgaatca aatgaaccca
    29821 tctgtactgc accgttactg tagctatatc cttcgcttga gtagccgaca tatcccatat
    29881 tgattttgtc tgtgataccg ctatctgcta aagtatcgac aaaacgatcg acaccaatct
    29941 ttacttcacc gatccgatta ttgtcgttca tacttcctga ccagtctacg accaatacga
    30001 gatcaagagg cgtgatatct ttttgtacat ttccacgagc attcaaataa acatcaaaca
    30061 atccgggtgt acttgtttcc tttgcatact ttcgtatcgc atagtctgct tgattaccgg
    30121 ttcctccata ttcgatatac gaatgggtcc gatcattagg atctccattc caagaattta
    30181 taccatccca ttgatttgtt ccgtttttat tgccttgatg atcaagaaca tttgtatttc
    30241 ctgtaggttg ccaactggct ttaggataag tgccctgatc atctgttgta tattctgtag
    30301 gaatcgtttg attagatggc acactcacag ctggcaagga taatgtaggc aatgtaaatg
    30361 ggctgacaga attttcgttt tcttttgatg attcttcttc tatttcgtct ggctttggta
    30421 ctgacacatc tgtatctttc ttattttctg tctcatcaat aatgtcatat tctttattat
    30481 cgacgatcag cttgtttgcc tggctgtggt aaagtgctgg atcgattgga aaactcagat
    30541 cgatagtttg tttagtgttt gatgagattt ccaacgtaat tttcccgcta tttacctgat
    30601 aagcatagcc atctccttct tttctttcca catcacttaa gtaattttcg actagaccaa
    30661 tttctttttc tatcttttca gcactggtgt tattcgcatt tagcttgatg ttcagtgctt
    30721 tttttccaga ctgatccgaa atgacttcag ctgaaattgt ttctaaaact gttggatcat
    30781 ctgtagtgtc tgctattgca gataatggca caaagtttga taaactcaat attatcagca
    30841 tgaaaataac tttcagtttc ttccctgtgg tcgtcatttt tcccctcctt tttctttttt
    30901 tctgttttga cgtgaatagt gaaaattatt aaaataaaca ggcgttacat aattttattt
    30961 attttaatat gataattatt atacgaaggg cgctaaaggg aatcacacca attttcatac
    31021 caattggttt catttatgaa gttgattcct tcgattaatg agcgtttcat tgaaagaaaa
    31081 gggttgaggg agaaaaacat atgatcaggt tgttggaatg aaactataga aagatggaaa
    31141 aaatcattcg aattctgtcc atttctgaca agtggatgac taaaaaagaa cttgccaaac
    31201 gaattggtag ttcagaatct acatttgtaa ggtatatcga agagatcaaa atccgctggg
    31261 gaacggatgt aacgattcaa tcttcacaaa aattggggta tcgtcttgaa catttgaatg
    31321 taccagctta tttaaatgtt ctgatggaaa tgtcgcaaag ttctacaaac agtcgtttat
    31381 taaatgagat tattgtcaat ccgggtaaaa cgattgagta ttattgtgaa aaaatttcca
    31441 tcagccggtc ttcttttaca cgtaagttga aacaatgcaa tcaaatttta gaaagctatt
    31501 tattaaaagt gatcgttgac caaggttttc agttaactag ccttaaaagt gagctccagt
    31561 tgcgtatctt tgttaccttt ttctttttaa gttactacgg aagacgcacg ttgccatttc
    31621 caatcgaaaa aaagaagatc aaagaaatta tggtcaagaa taattgtcag atgaatttgt
    31681 ttcaggaaga aagcccatat gagcaatctt tctttattat gtattttgtt gtgcatctca
    31741 tgagagaatc ccaaggaact ccatttgaat ggaaagctgg agactcttat gtacagttga
    31801 taaatatcga taaaaaagac ttcttaatct tgaaaaatct cttaggaaaa ttgaagtaca
    31861 gcagctatat aaaagtagtg aactattttg tcaatacgat tttttcagaa ttttcggaga
    31921 aagaaaaaaa tgaaatcagt gagcagatac gaaaaaatat gtcctactgt tcgttttcat
    31981 ccgctgattg caaagaaaaa aacgaagaat atgttgttca attattaacg caaatttatt
    32041 ttttctcccg tattttccca tacgatacga ctcatttgac gatccgtgca acttactttg
    32101 cacagcagat gcaacaccgc cagatacgtc tatatgctgg atttcgtgca gaacttcaat
    32161 tttattccac tctactgaat cagaatctga tttattattt atctagctat atcttttgga
    32221 tgcttatcgg taatcctgaa attcaccatt atacttctga taaaaaaata ctgattatca
    32281 gcgacttaag tcttcgacat agtcaatata ttgaagaata tatcagtgat attcttgctg
    32341 tgcataaaat tcactctgag acaactgcta ttactgaaga tcagctgtcg aaatacaatc
    32401 ttctcgagta tgatctgatc gttacgaatc aacccatttt gaatgcccat gttcctcata
    32461 tattgattga tgatagtgtg tcattcgcaa atgaagaaga actcatccgc ttattcgagt
    32521 tatgaataat caaaaaacag ctatgtataa accaccctga taaaaatttc aggacaaata
    32581 cacatagctg tttcttttta ttgtattcct tcagcaatcc aacgagattt atagcgaaag
    32641 tcttttaaca actcttcttt tgtccaatta tttgcaatga gatccgtcaa agtgttcatt
    32701 acgagggtca tggaaaagta taggaaacta actgcttctt ccgacgattg aatggagaaa
    32761 tgattgattc ttaataattc cagccatttt tcgttcattg gagaatcagc cgatagttct
    32821 ctacgtttgg ttgtctgctg atcatttcgc tgggttaaca agtataacct ttgccaatag
    32881 atactttcgt gatctaagtc gcagcaccac tctaaatatt tttcgatccc caaaaagaaa
    32941 tgttgtttgt tttcgctaat gaattttaag atatcttgat ggactatgtg agaactgctt
    33001 ttgatcgtat acaaataagc atcttccaaa tcttgaaaat atttataaaa agcaccgcgc
    33061 gacatcccca tcttgtcaac gatttctgag actttgactt ggctgatgtg ctgtgagcaa
    33121 aatatctcca gtaataggtc atcgattctc tgtttctttg cgttcgataa attgaaaaaa
    33181 gtttcttttg gcatccacat ctctccttta tctctgatta tacgtggata gcccttcatt
    33241 gtcatgaagg attcattcct tctttttctc ttcttctgtc aagtacccat cttccattcg
    33301 ataaacgcga tcactccatt gaatcatacg gacatcatga gtaaccataa tagtcgcttt
    33361 ttgtttctca tgagcttctt taactagtaa tttgacgact tcatatgcat gatctgtatc
    33421 caaactagct gttggttcgt ctgctaagat caagcttggc tcattatata aagcgcgagc
    33481 aattgccact ctctgtcttt ctccaccaga aaggtcttta ggataactat ttttaagtga
    33541 ctggatatct aaagattgca gcaaggaatc gatttttgtt gaatcttctt tttttgaagt
    33601 aacttggtca actagttcaa attgttcctt taccgttaaa aaaggaatca aattagagct
    33661 ttggagaata aatcctattt ctttaaagcg taaatctgct cgtttttttt ctggaagctc
    33721 agttagtgag tgaccgttga tcactatttc tccggaagtc ggtgtttgca atcctcctga
    33781 aatggtaagg aaagtacttt tacctgaacc agaaggacca atgatgctga cgaattctcc
    33841 ttcctttaca taaaaatcaa ttgctttcaa agctgagacc tttgtatgac cagaaccaaa
    33901 tgttttggta acttttttca tcgttaatat ttttttcatt tttattaacc tcctattgct
    33961 gtaattgggt ctactttggt tatcgtacgt atcgaaaaca atccgcctag cattgcgaca
    34021 atgatcaaaa tcccactata tagcaaccat tgtgaccaaa aaacagcaaa aggcatcgcg
    34081 cttggtaaaa tcagactagt caaataagca aacaaaagag caagcagaac gcctaccacc
    34141 ccaacaataa aggactgtgc tactaaagat ttagcgatga agaaattttt gatcccttgt
    34201 gctttcatta ctccaaaaat agcagtcttc tgaagtgtca tcacgtacat gaaaataccg
    34261 accacggcag caaccactaa gaacaaaaag tatatcatcg catttaaggt catattttgg
    34321 gcagaatagc caggaatact ttcaatgaat gtcccaatag ttaataactg tagacccttc
    34381 gatgcttttt ctttcgaaat tttagctgaa tcctttatta cgatcccatt gatcggctgt
    34441 tcttcttcag aagcaaacgg ctgttgtcca tacttcaaag cagtccacgt atctagggac
    34501 gcatagatga cgggagaaac tgtgtaataa gtttcaggaa agataccgac aatcgtcaaa
    34561 ggatcgtcgt aactgccaat tttgatcgta tctccaattt tatagcctga atcagccaga
    34621 ttttgagaga tgatgatttc attctttgtt ttaaattcat gccctttcgt gagttttggt
    34681 aataaaaatg cttctttcgt tgtgccgaaa actgtaacat tttctttgtt ttttcctttg
    34741 attgctccgc tgtacaatcc gattggtgct ttctctcctc cttcaatgta tttcagatct
    34801 cctcttgtta attgtgaagc tgcaaatact tgatttgcat cctccgacaa aacaatctct
    34861 tgtgcgtccc aatcatcgat tgctttttta aactcttctg aaagtccatt cgctagtcct
    34921 gacaacatga atacgacata cgcaatcaag aacataatac cgacgatcaa gccatatcgt
    34981 aatttagaat agcgaatttc tttgatagct agaaacattt gtttttcctc cctatgatag
    35041 attgatagtg acaaaatgtc acttgttgaa tagtgtaact ctaaagtgac aatttgtcac
    35101 ttgtgttgtt tttttcttat caataaattg tctaagacat ctttcgataa aaccttaaca
    35161 tgagtcataa aaaatttatt taagccttat ttatgctttc catgataaac taaagtgacc
    35221 aaatcaatga ggtgggaaaa tgattaaact attagtagta gaagacgata aagtattatc
    35281 caataatata aaagaaatac tcgctgcaat cggcgagata acacaagttt atgatggtag
    35341 tgaaggactg ttcgagttgg aaagtggagt ttatgatctt gcaatcttgg atttgatgat
    35401 gccggaaatg aatggatatg aagtgttagc aaaaatacgg aaagaaaaaa tacagactcc
    35461 agtattgatt ttgactgcaa aagatagttt ggaagataag attgaaggat ttgaacgagg
    35521 tgcagatgat tatctaacaa aacctttcta tcgagaagaa ctgatcatgc gcgtgaaagc
    35581 attattgaaa cgctcgctag gtctctttga tgaaaatgtt ctgaagtata aggatatcag
    35641 ttgtgattta aaaagcaatg aagtcttgta taaagaacaa gttttgccca tacaaggaaa
    35701 agagtttgaa ttgctcgttt attttttaca aaataaggga atcattttga caaaagagca
    35761 gatatttgac cggatctggg gatttgattc tgatacgacg attacagtta ttgaagttta
    35821 tatgagcaac ttacgaaaac atctgcgaag tactggattg gataaagaaa tcaaaacctt
    35881 acgtaatgtc ggttatatac tgcaaagggt gtgatgcaat gaatggagat attacacgaa
    35941 agcaacgtag tcgctttttt gcgacaaatg tagcagcatt tgcttttatc ttttttcttt
    36001 tgggattcat tactttacag atattaaacc aatctgcata cagagaaaca gatctgtcat
    36061 tagagaaaat aaattttgat tctcgaacag tacaattaga gatacaaaga tatcagcaag
    36121 gcgatccctt tttaaacccg actcaaacgg aacaaaacag aacaaacatg gaacagccat
    36181 taatggaacc atcaaatgac cgttttaaca ctcaagtgat cttgtggtct aaaaatggtg
    36241 agatattgaa taaagcagct attggtgggc gtttcaacca aatcaaagaa ttaaaaatgg
    36301 atacgaaaaa tctgaatacc atccaaaata ttgaattaaa tgaagacagt ggctcatctg
    36361 ccctctcttt ccgttcgatt acgaaaaaag cggagacgaa tcaagatggc gttgcctaca
    36421 tccaaatttt agaaaataca aatcaaatca agaattcatt agcgactttt cgcacgatta
    36481 tgattttgtg tatgattgtt ttttggattc tctctattgg tatcagttat tacttgtcta
    36541 gtctaagcat gcgccctatt attctttctt ggagaagaca aaaagaattt gttgagaatg
    36601 cttctcatga attgcgcaca ccgttaacga ttattcaaaa tagtttggaa catcttttta
    36661 ccaagcctaa tcatactatt attgaagaat ccgaaagcat cgctcaagca ttgtcagaga
    36721 cgaggcgcct cactggattg acttcggatt tgctgacaat cgctcggaat gattctgatc
    36781 agcagctgct ttcaaagcaa atgatcaata cacaagaata tatcgaacaa cttgttaaac
    36841 catttcaaga gatggccatc atagatggaa aagagtttat tctccataac gatgcaacat
    36901 tagaagtacc aattgatgaa aaaaaaatcc atcaagtttt ggttattttg ctggataatg
    36961 cattaaaata tacgaatcca gaagataaga tcattttgga ttcatctgtt aaccataaag
    37021 aatggctgat tcacgtaaag aatactggcc cttctatcag tgaagaagat caagcaagga
    37081 tttttgatcg attttatcgg gaagatcaat ctcgggcgaa agagactggc ggatacgggc
    37141 ttggcttggc aattgcaaaa caggtaatcg aacagcatca tggacagatt tctgtatcca
    37201 attatcttcc acaaggagtc gattttaaag tccaattgcc gttgaagcct aaaaaataat
    37261 gcttatccct aaaaaagggc tgtgacaaaa atcttgtcac agcccttttt tgttgcgaat
    37321 aaacggtgtt ccagaagtag cttcttcgga aataagccga aatttcttta agctgaccac
    37381 ttctgtcaca acctctttag cttacgctta cgatttttta ttgcggtctt caatgtgtac
    37441 atcaccgctt gtgtcaatat ccaattcttc tcggctaaca tcttctgtca ctttttcaac
    37501 atcttcatgt tcttctttat gaatcccaac ttcttcacga ataactgtat gtttgcttac
    37561 atctacttgc tcttctttga ttgggatagt aatcgtgtca tcttccaatt tttcgtctgt
    37621 tccctgtgaa gattgatctg ttactggttt gcgttcgatg acgatttctt cacgtttgac
    37681 cggtacttct accgtttcag tatcgttgac cacatgtttg tggatgtcga cttctccagt
    37741 tgtcacatca tgtgtattta catctaattt ttcttctttt agttgaattg tatcatcatc
    37801 tagatttggt ttgcgatcga tgtcttcagt cttgcttgta cccattgtat cagtctcgcc
    37861 aggtacgtcc atcggatcag ttcctggtgt catattcaat ggatccgttg tattttgaaa
    37921 gcctgtcgtg ccacctacgt ccattggatc tgttccagtg ccagtttcta atccagtccc
    37981 cattgtgctt gctgggctaa cagtatcttg tgtatccatt gtatcttctg ttgtttctgg
    38041 ctgacgatag ccttttactg tgatgacaaa ttttccatca gaaatatctc ctctatattg
    38101 atataaagga tcattttctg gtgtagccgt ctcagaatca taggtatcaa aagaaaaagt
    38161 atcttttatt ttttcccata aagaacggtc ttcttcacca tccatccgat tttcatctgt
    38221 ttcaacatct ataccagaca atccgtcaaa tcctagtcta gatgctgctt catcagtagt
    38281 ataaataacg atatcatctc gttcataccc ttcgttacgc aaccgttcaa ttactgataa
    38341 tgcttcttcc ctcgtactat agcttccgat gaccgttaaa tttttctctt ccatgtaaat
    38401 tcctcctagc aaatagattt atgattggta tattgttact tataaattta ttctatagga
    38461 gacgattgtt ttctacaaat atttagattg gaatttgatt aaaacaattt aattgaaatt
    38521 tgaaaacatt tatgttaatg aaagtcatat tctaataaaa tgtcatttgt ttctatatga
    38581 atttttatga aaaatttcat ataatttcca ctcgttgtga tggtttaatt tatccaatcc
    38641 atcaataagc aaacaactta tataaaaaag gattaattct caatataatt gctcctacca
    38701 tagaataaat cttcatgtaa agtaatgaag taccaactat ttctaatact tctttaagaa
    38761 ttatcccaac aatatcgtta gacaaaccat tattgaataa atggagagcc aatcgattct
    38821 gaaaaaatgt tttctaattt ttcggaaata aaagaaagag cctcctgcca ttagaacgaa
    38881 ccaaatccat ttcattgctc atgaagtaac cacatagcta aaaagaggat aaatcaatta
    38941 aaagatcaaa agaatgactg cactaataaa ataactaaaa atgtatcttc ttacttcata
    39001 tcttttagct agtacttagt taaaaatttg cttcctaaga tcaagacaaa ctgtctatct
    39061 tcatttgatg aaatgcggta aaatatagaa aatgaacact attggaggga atttgtaatg
    39121 gacagcaaaa ctagaaaaca actgcaagaa aagttaaagc aacgtacaca cttgctccct
    39181 tccgtctttc aacagttttt agaagaaaat cgttcttccc tttcattagg cagtcgctat
    39241 gaatacgcaa aagattttca tctgtttact gattatttga gaaaagtatt ttctgaagaa
    39301 gtggatgatc agctgattat ccatttagaa aaacaagatt atcagaattt cttagctaaa
    39361 atctatcgac atactcgttc gttccaaact acagcgaacc aagaaacgga gcaattgttt
    39421 gaaaatagct attacgggat ttcaaggaag caatattcat taaatcattt tttacgattt
    39481 ttgtatcaaa aaggactgat tgaagaagag atttctcttt tgggtacaag tttaccggaa
    39541 acaaccaaag cagctccgcg atatatcgat gcagaacttt tcaaagattt tgagcatttg
    39601 tttatcccga ttgaaggctt tcaaacaagc agagaagcta gctttgaaga aaagaataga
    39661 ccgaggaacc ttgcaattac tgccattctt ctgtattgcg gattaaagat ccatgaagta
    39721 gtagaactta aacgtaaaga tgtatctttg accaagcaga ttcttaatct gaatagaaaa
    39781 gaaaacagac tgaataaagt accaatacca aaagaagcga tgccatttct ggagaactat
    39841 tttaagttaa cagcacaaaa tacggacgaa aattcttttg tttttcgttc ctcacatgat
    39901 cagcaaattt ctcctcggac tattcgccaa attttgagca aaattacagt atataaaaac
    39961 gtatcgccag aactatgcag aaaaacatat cgttattatg cagaactata tttagatgat
    40021 gctgacgcgg taaattattt atgtggtaat cgatacctct catcccattc ttttcaagat
    40081 atctgggaaa agatggttga tttttcttat cacgaattag cggcattcgt tcgggtttct
    40141 tccatataaa acaaatgtat gatctttttt tcacaataaa ctatgttatt atataaagga
    40201 ttatgttacg attatcttga atttgtatat tacttaacaa aagataatgg agtgtgaatg
    40261 atggagacca taacgtattc ccctttgaac ttatttacga attttcaaga agctgcagct
    40321 aacttttccg agaccccgat tatctttgat gagccacttt cagctttccc agaattagga
    40381 cttgaaacaa cttacaaaaa atgctgcgaa agtgtgttga aacgggctta tcaattggct
    40441 catcttggag tgaaatccgg agacaaagtt attatttata agagttccgc ttttgacaca
    40501 taccttctag ctgtttctgt gtcttacctt ggtgcagtgc ctgtcatgac ttcttatcat
    40561 ctaccaacaa caacaatgga agtatttatc gatcgtttag aagacccttt cattttattt
    40621 gatgatgaga caaaaaagcg agtgagagaa atttcaaatg gaacaaaatc aaaacagatt
    40681 c
//
LOCUS       DACDYI010000019        38235 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.019, whole genome shotgun sequence.
ACCESSION   DACDYI010000019 DACDYI010000000
VERSION     DACDYI010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.019"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(451..1761)
                     /locus_tag="IWM52_000956"
     CDS             complement(451..1761)
                     /locus_tag="IWM52_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP9973227.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3191)
                     /locus_tag="IWM52_000957"
     CDS             complement(1773..3191)
                     /locus_tag="IWM52_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9973228.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3431..4999)
                     /locus_tag="IWM52_000958"
     CDS             complement(3431..4999)
                     /locus_tag="IWM52_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAP9973229.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5003..5413)
                     /locus_tag="IWM52_000959"
     CDS             complement(5003..5413)
                     /locus_tag="IWM52_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP9973230.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5410..6333)
                     /locus_tag="IWM52_000960"
     CDS             complement(5410..6333)
                     /locus_tag="IWM52_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAP9973231.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6330..7208)
                     /locus_tag="IWM52_000961"
     CDS             complement(6330..7208)
                     /locus_tag="IWM52_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP9973232.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7195..8532)
                     /locus_tag="IWM52_000962"
     CDS             complement(7195..8532)
                     /locus_tag="IWM52_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP9973233.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8535..9638)
                     /locus_tag="IWM52_000963"
     CDS             complement(8535..9638)
                     /locus_tag="IWM52_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP9973234.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9712..11220)
                     /locus_tag="IWM52_000964"
     CDS             complement(9712..11220)
                     /locus_tag="IWM52_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9973235.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11186..11878)
                     /locus_tag="IWM52_000965"
     CDS             complement(11186..11878)
                     /locus_tag="IWM52_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9973236.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11900..12874)
                     /locus_tag="IWM52_000966"
     CDS             complement(11900..12874)
                     /locus_tag="IWM52_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9973237.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13174..14046
                     /locus_tag="IWM52_000967"
     CDS             13174..14046
                     /locus_tag="IWM52_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9973238.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14504..16702)
                     /locus_tag="IWM52_000968"
     CDS             complement(14504..16702)
                     /locus_tag="IWM52_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9973239.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16934..17926
                     /locus_tag="IWM52_000969"
     CDS             16934..17926
                     /locus_tag="IWM52_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9973240.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17986..19407
                     /locus_tag="IWM52_000970"
     CDS             17986..19407
                     /locus_tag="IWM52_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAP9973241.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19400..20899
                     /locus_tag="IWM52_000971"
     CDS             19400..20899
                     /locus_tag="IWM52_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAP9973242.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20938..22332
                     /gene="uxaC"
                     /locus_tag="IWM52_000972"
     CDS             20938..22332
                     /gene="uxaC"
                     /locus_tag="IWM52_000972"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP9973243.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22491..23174
                     /locus_tag="IWM52_000973"
     CDS             22491..23174
                     /locus_tag="IWM52_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAP9973244.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23176..24012
                     /locus_tag="IWM52_000974"
     CDS             23176..24012
                     /locus_tag="IWM52_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP9973245.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            24023..25777
                     /locus_tag="IWM52_000975"
     CDS             24023..25777
                     /locus_tag="IWM52_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9973246.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(26053..26394)
                     /locus_tag="IWM52_000976"
     CDS             complement(26053..26394)
                     /locus_tag="IWM52_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP9973247.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26462..28633)
                     /locus_tag="IWM52_000977"
     CDS             complement(26462..28633)
                     /locus_tag="IWM52_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP9973248.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28844..29701
                     /locus_tag="IWM52_000978"
     CDS             28844..29701
                     /locus_tag="IWM52_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP9973249.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29772..30629)
                     /locus_tag="IWM52_000979"
     CDS             complement(29772..30629)
                     /locus_tag="IWM52_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9973250.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30614..33316)
                     /locus_tag="IWM52_000980"
     CDS             complement(30614..33316)
                     /locus_tag="IWM52_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP9973251.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33329..34618)
                     /locus_tag="IWM52_000981"
     CDS             complement(33329..34618)
                     /locus_tag="IWM52_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAP9973252.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34768..35814
                     /locus_tag="IWM52_000982"
     CDS             34768..35814
                     /locus_tag="IWM52_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9973253.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35816..37969
                     /locus_tag="IWM52_000983"
     CDS             35816..37969
                     /locus_tag="IWM52_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP9973254.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT        11282 a         7860 c         6802 g        12291 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa
     3301 aaacaaaaaa taatctaaaa acattgatgc ataagattga taactttttt tacataacaa
     3361 tatatacaaa caaaaaaatt cactaactag tgaaaataag ttagtgaacc tcatttatta
     3421 aagaattaaa ttacagatga tcttttccat catacttgaa aaactcaaaa tcagcataac
     3481 ttcctctttt tttatttaga tcatgcactg ctatcccgat aaaatttcca gtaaaccctc
     3541 ctgagagtat acacatgttt ttttgcggac ctactttctt ccattcttca gttgatacgt
     3601 ctttcaaatg aaactgtgct gtctctttct ttacagtgac tttcaaaaaa gtcttctttt
     3661 ctgtgagtgg aatcgtccaa tcttctattt gtgctactcc gttttctgat tctatcatac
     3721 gtaggcattt tcccttgtcc tcattccaag taacataaca ataaatataa ttggattcat
     3781 ttaagtacag taccagtcca gccatctgat taaaattatt aggcatgtat tcaaatgctg
     3841 tctccgcatc aaaagtaaaa tccgtttgcc gaatagctaa caaatgatgt tcgaaggtag
     3901 attgaagaga ttcaccggaa atcagacgta aaaaacctgg tctacatttt gtatcacacc
     3961 aatcagcatt tggcaaaatg cgtctggcat tccagttctt ctttagctct ccttcaaatg
     4021 tatccataaa ctgatgatct ttttcttgtt gtacctcttc atttgtctca atcattattt
     4081 cttccatcgg agttgtctgg ctatctttaa gtcttaacca atcgtcggac gtccaataca
     4141 cttcttgaat agcggtttct cttcccaaaa tagccgcacc gtttaatggt ctggtacaaa
     4201 gatatgtcat gtaccatctt cctgacgggg tttgtactaa gctcccatgt cctgtacatt
     4261 gcaatgtaga agcaggtttg tcactagctg tcagcatagg aaaaccagga tcaagctcgt
     4321 aaggaccagt aatattcttt gctcggcaaa ctgtaactga atgtcctctt cctgtaccgc
     4381 cttctgccgt catcaaataa taatacccgt tacgaaaata caaatgtggt gcctccgttt
     4441 tcgctaattc tgtacctgca aatattttat agatgggacc atcaagcttc ttcttctctg
     4501 catcatattc ttgtaatacg ataccgctgg atttattcgg tgtgtccaga cgataatccc
     4561 atagttcatt gacaagccat ttttttcccg tttcaagatc atggaacaaa gaaggatcaa
     4621 agccgttact atttaaataa attggttcgg accaaggacc tgtgatctca gatgcagtga
     4681 tcaaataatt gtggacatct ttaaacggac ggcctgcact tttgacatct gtgtaaacaa
     4741 gataaaacaa tccatcagca taactgatct gtggcgccca tatacttcca tttgtaggat
     4801 ttccttgcaa agatacttga gtggtcaaaa tatccgttgt atgtgaccag ttgactaaat
     4861 ttttcgactc atatactcgg attcctggaa gccattcaaa agaggagaca ataatgtaat
     4921 accgctcttt cactcttaat atattaggat ctggattgaa tcctcttaaa ataggatttt
     4981 tcactttgat agtctgcatg attcaggctc cttttctcat tcttactcga tataaactga
     5041 atgccccagc tgcgaaataa aaaaatgcaa aaggaaacgc aataaactga gtgccaatca
     5101 acaatatgaa tcctgttata aaatacaaac cagtggcagt tgttaagtat tttcctttag
     5161 cagtcaaaaa caatgctact gctaaaaaag caagtgaaat aaacaacaca acggcaaaca
     5221 gaccagtcat gtcctgtaat aatccaaatg tattttctaa agacaaagtt gaatgatcta
     5281 cagcaagcga ctgataactt tttttaaact ctgacgcaga catattactt aaagtaactg
     5341 ctaacccgcc aaagaaaatc aaagatagac tgccaccgac cattcctagc aatagttccc
     5401 attttctttt catttctgaa ccccctgctt ttctaaatgt atccattcag gtcttttgat
     5461 cgatgtttca taattgttat tacgttcaga gaatttgaca gtctgttcat ttttcttgtc
     5521 atcccaatta tcccaaccag ttgggtgaac atgcccacct atcttacacg cttcaaataa
     5581 gacatttgca tacggacgcc acggtcttcc taaatagacg tttctagtat caggttctgc
     5641 agtcaaggta cagctttcaa aacgataccc tgttttttga tttttcggcg tagatgctgc
     5701 tgtgatgtat ccaggatttt tactggctaa gctatggata tgacactgat gaaaaacggc
     5761 atctgcccca ccaaatatga aatccaccgt tccttcaatc cagcaatatt cataggtctg
     5821 acgacaatac tcaggattgg cagaagccgg tgtacaaaaa ggagtaccat ctttttgcaa
     5881 atcaggcaac ggacccgtgc ataaggtatc ctgataacca gatagtcgac agttttttaa
     5941 agatacgcga tctcctgaat tgaataaagc aacagcctgt cctacctttt ctcctggacc
     6001 tgctgtattt tcgatgtgaa gattttctaa tacgacattc gatccttcta agaaaaaagt
     6061 agcacttcgg aaagtcccta actggctacc gtcaagatac tcctgttttg caaaacgatt
     6121 accggagata actactgttc ctgctcccac aagtttaaaa ttggataatc tgctttctat
     6181 atattcatca tatcttcctt cttttactat caagaccttt ggatggctat ccggcaggcc
     6241 tgccaaataa tcgattcctt cttggatagt ttcaaaatcc gtcggggtat ctatagcatt
     6301 ctttccaatt atcagtttgt ctttcatcct cataaatgct cccttatgaa ttgtttccaa
     6361 atatttctca tttcttgtgt ttccatatga ccgcctgtta aagaaaaaga agaaaagttt
     6421 tctggactac ccattgattg atactttttg tccagccctt ttctcagctt cagtacgcct
     6481 tcgtccaagc acatcgtatc gttttttcca actaagctaa gacgagggcg cggagcgatt
     6541 ttttcttgga ttgctaacgt tgtataagct gtcaatagat ttggcacata gtaataaaac
     6601 ccatgatgat ctaggcggcg atgtagcaaa agtatttcga tgtccacctg tgccgctaga
     6661 tcaatacaaa ctttgacacg agaatccaat gctgtcaccc accagcttaa taagcctccc
     6721 atagaaagac caatcgttgc gagccgttga gtatctgtgt cttcccttgt ctccaaataa
     6781 tcgatcaagc tgatcacatc ataaatacgc attccccata acgtttttcc tgttaataaa
     6841 aattgtttga acaactcgct ttcagaaata cctccgcgtt cttcaaatcc ccaagcatca
     6901 atcgaccaaa cagcatatcc cattcctgtc agtacttctg caaaagatgg agaagctaag
     6961 taaggagcac catggatcaa ttcacttttc ccgactgaaa aatctccgcc gtgagaatgc
     7021 aggtaaagga ccggcggaaa cggtccttct ttttctggat aagcatagag ggctggtact
     7081 gtttccaatt catttaagta caattgatag tattcaagca taaaaccgtt ttgtttttct
     7141 cggtataatc gttttccact aggggtatgt ctgctagatg gagcacctaa aaaatcattc
     7201 aactgcataa gcaaagtttt tacgaacaac cagttcatcc atattttctg attgatttga
     7261 ttggaatttc tcaatactga tttctttagt attttctaat agaaatgctg gtccctcgtg
     7321 attttcaatc gacatctggt taaaggaaac cttacccgca aagccaatat aaaatccttg
     7381 tctgctcatt tcctctattc ctgtcatcat agcaggaaca ccgggaactg catcttgggc
     7441 catcgagaca tcgatattgt caaatgtgat ttcttgtatg aattgttccg ctaatccata
     7501 aataaaacct gcagaagcat ggacattcct tgccgtgata ttcgaaaaat ggatccttct
     7561 gaatgctggt gtccgttcat ctatcggata agcctttttt tcccatacat acggttcttt
     7621 ccctcgcgga ccacagaaat aataaagatt cagtataaat ggacacatca cattatccat
     7681 gactatattg gatactcgaa tatcttcaac gatcccgcct cgacctcgtc tagatttcaa
     7741 tcgtattcct cgatcagttt cttggaagat acaatttgaa atggtaatat tacgaatact
     7801 accactcatt tcacttccta atacgacacc gccatgtcca tggaccatcg tgcaatttgt
     7861 gatcgtgata ttttcgcaag cgatcctttc gtacgtatct tcagtccctg ccttgattgc
     7921 aatacagtca tctcccacgt caatatggca attactaata cgcacatttt tgcatgattc
     7981 tggatcaatt ccgtctgtat ttggtgaatc cgctgggttc aagatcgtca agttatcaaa
     8041 cgttgcatta ctgcaaagaa tcgggttgat cgtccaactt ggtgactgga tcaatttaat
     8101 atctttcaca gtaattcgat gacagttatg gaaactcatc aatttaggac gaggataagc
     8161 gagattatct ggttcatttc taaaagtatg ccaccatttt tttccatttc catctaatgt
     8221 accgaaacca gtgacagaga tgttctctac attttgagca tagatacagg aagcataaac
     8281 ttttcgatgg actccttccc aacgtgaatg aacaacagga taatcttttg gatcgtcaga
     8341 gaatttcaga accgctcctg cgcttaaatg aagttcgaca ttgcttttta aaaataaagc
     8401 tcctgttaga aattcacctg ctggaacaac tacggtcccg cctccgtcac ttgctgcagc
     8461 atcaatggct tgttgtattg cttcagtatt caattcatca attgatgcgc caaatgtcaa
     8521 aatatcatac atatttataa tgcctccact tcaatcaatg cttgcaagaa tgcaccaacg
     8581 cctttttgat cattacaaat aattggttca ctgatgtagt aagcatatga accatctcgc
     8641 tgatctgctc cgcctaaccc tgctacttga cagtttttat tcaagttcag ccagccttct
     8701 tttgtttcca gaacaaattc atcaagtaat ccttgatgtg atttcttcac ttgtggaagc
     8761 cattcttctt tgttcagcag tcccaaacgt attcctttag ccattgcgta aacaaacata
     8821 ctactgccgg aagcttccag ataatttcct ttccgttttg tttctgttgt tacttgatac
     8881 catgtcccgc tgttttcttc ttgatatgga agtaatgcag ccagtgtctt ttctagaatt
     8941 tctttcagca aagaacaatc tgtttcttca tgaagtaatt cataggtatc tgctgcagcc
     9001 atcaaatacc agccaatcga tcggctccaa aaatgatggg atagacctgt ttttggatca
     9061 gcccatggtt ggaccttctt ttcatcccat gcatgaatca gcaaatttgt ttttggatcg
     9121 aatagatgtt tataactgat ttcaaactgt ctcaaaacat cttggtaatc tttttcttca
     9181 ctaaaattct ggataaattc agcataaaaa ggagaaccca tatataagcc atccaaccaa
     9241 atttgataag gataaatttc tttatgccaa aaagctcctt cggatgtgcg tggatggttt
     9301 gccaactgct gggaaaggag ttctgctgct tttttatatt tttcttcgcc tgtttctttg
     9361 tacaataaaa agaaaagctt tcctgtattt acatggtcga tattgtattc ttcaacgctg
     9421 tagccacgga tcgttccatc ttcttgcacg aaatagtcga tattgtcttt tatgaaatca
     9481 aaataaattt tttcttgggt tcgttcccaa agtaattgga accctttaag tacgacgcca
     9541 tagtcataag accattttcc tttataccac ttttcttcat ataagcgagg tgttctttcc
     9601 atgatacttt ttgctaattt ctttgaccag ttttccatta tttgctctcc tttaaaaatt
     9661 aaaataggat ttgggacaaa taccaagaag tatttgtccc aatcgtctat attactgtgc
     9721 cgctgctttg tatgcttcct cgtactcttg tgtgattcga tcgcctcctt gtgctttcca
     9781 gtcttctaca gcttttttga atccatcttc gtcaagctgt cccatgatgt attgcaccgt
     9841 agcatcaatg acgatttttt caatttctga tcctacttca ttgttcgtcg gtgaatccaa
     9901 tggcacagaa ggatccatta cagcaaattc ttcattttct ttgatcaatt gattagagaa
     9961 ttctttttca gggtctgtat ctttgaaaga gaacgtcact tcactaggac gagagcttga
    10021 taacggttgc acctcttgtt gccaaagatc catatcgata aattcgatag aaccatcatc
    10081 attcaattca taatgcgttc cttcgatacc gccagtcatc aatttataca catcttcttc
    10141 taaaagatca tcaacaaatt gaagtaattt cttcaattgt gcttcgtctt ttacttgaga
    10201 tttgggaaat gctagcaagc cgccaatacc atttccttct gaccaaacat gataaccgcc
    10261 gccttcacca tctgagatct tgtttacagg cactaattcc atatcatctt gaataccttg
    10321 acccatcgtc ttcagattct tgatatccac cattccagtg tagattccag ttcttccttg
    10381 tgcaaattgt tcttgctgat cagtcttttg ggtaactgca aagtcttgtg ccaaatagcc
    10441 attctcaaat agatctcttg aaaaattcaa agctttcata tactcttctg tatcaaattc
    10501 tggagtgaat ttgccattat cgtctacagc ccagccattt ggtgctccaa aataagaagt
    10561 gaaaagttta aagctggtat aacgaatatc actaggcgca cgatcgccaa aaccaactgt
    10621 gtcgtccaca ccatttccat caggatcgtt ttctgtaaat tgtttagcta cttcataaag
    10681 ctcatccatg gtagttggaa catctagtcc tagattatcc aaccagtctt ttcgaatgac
    10741 taagccagca cgagcgtagt ctttttgtat cggtacgccg tacaaggcac cgtcgatact
    10801 cgcagcttcg attctatctt cagaaatttg acttaaattt ttatagtctt ttaaatattc
    10861 tgatacatcc cagaatacgc ctgattttaa cgcattgcgg acagaagagt ttttcaacat
    10921 cgcaatttga agtgtaacga tatctcctag ttcatcagaa gccaatgcag ttgttaatct
    10981 ttcttctttt gaagcatcag gaatccagtt gaatgtcagt tccgtatcgg ttttttcttc
    11041 gatcaagttc acaatcgtgt ctgtaggcgg tgaagcatga tgcaaaatat ttaaccaagt
    11101 gattttagct tttccatctt ctgtctctga agaactgtta cttgaactgc tgccgcatgc
    11161 ggcaagtgct agaattcctg caaaactaag acctaatttt acgagcgttg cttttttcat
    11221 aaattttctc cttcactgtt ttaataatat ttttgttttc ttctgttttt tctgtatatg
    11281 agttcttcca ttccaaatgg caaaatatag aatttgctgt ccacgtgact gcggaaaaga
    11341 agagactgcc accgataaag gttaaaacac ctggcattgt ttggatgatc atcagataaa
    11401 tccctaccat acttaatatc aacactaatg agtaatgaag atgtgagaat ccgaataaaa
    11461 atgccatttt cattttgaaa aaatatcctt tccaatcata atgagccatg attgggaaaa
    11521 tatagagtgc tgagagcaca tatatgaacg cagcaatata caaagcgatc ctaagatacc
    11581 aattgtgtat gaaagaaaga tccacaagta aaacaatccc gatggcgcca tatatccagc
    11641 tcatcagcaa ggtttcacga tagttttctt taaaatatgt gaagtacgtt ttcgtcaaag
    11701 gaaacgtttc ttttgagcgc agccattttc taatcactgc aaccatcgca tacgatgcag
    11761 gaccgaaagt aaaaactccc aaacctgcga tgactaaacc gccccacaga aaattcaaat
    11821 aaaccaactg taaaatacga tttagccttc cgtttatttt atctaatgac tgcagcatat
    11881 ctttccctct ctttctatct tagtaaacgc cgtcttctcc tagctttttg gcaatcttat
    11941 tagctaaaat taccataaac aatcctacga cccctttaaa caatcccatt gctgtactga
    12001 aacttaactg tccattcttc aatccagctg tataaatata ggtgtcgaaa atttctgcaa
    12061 cgcttcgatt caatgaattc aataacaagt acatatgctc gaaccctaag tctaatgtct
    12121 gaccgatttt tagaatcagc aaggtaatga ttactggacg aattgccggc aatgtaacat
    12181 gccatgtttt gcgtaagcgt cctgctccat ccatttctgc agcttcatac aactgtgtat
    12241 cgactgcagt tatcgctgaa aggtagatga tcgtagacca gcccaactct ttccagatca
    12301 cttgtccgat atatactgtg cgtgtccatt caggggatgt tagaaaactg attttttcaa
    12361 atcccattgt tgcaaggaat tcattcaata cccctccgtc aacattcaaa aatacataag
    12421 taatcgaaac aatgattacc caagacatga agtgagggat gtaaatgatc gtttgaatcc
    12481 cgtttttcaa tcgtttgttc gttacttcat ttaatagtaa agacaaaatg attggcatcg
    12541 gaaaaaacac gaagatattt aatccgaaca agatcaacgt atttcttaat aatgtaaaga
    12601 atgtcggttc tgtaaacaaa cgaatgaaat gtttgaaacc gacccaagga cttcccatta
    12661 ttcctaaaaa tggttgataa tcttgaaaag caataacgag tccccccata ggaagatacc
    12721 taaaaatcaa gaaataaagg actcccggca gaatcatcac atataaaaat ttattcgttt
    12781 tgattcctgc aattattttt tttcgtcttc tgagcttttc ttcttttttc gatagcgtca
    12841 gttgaaatgg ttcttgtttc tttacacttt ccataagccc ctccttagtt ttgtgaatgt
    12901 ttgctgctta tgacttcatg atagataact atctgaatta cgtatataat catttttacc
    12961 gtaagtaaaa acgcttacat agcaatgatt tttgtgctat acaaaacgat aaagtgtatt
    13021 ttttcacaaa taaactacgc atagcaaacc ttctattttt ttctatctga cacttagcat
    13081 aggaaatgat tatatacgta atctttcacc gatgataaat tgaacatata gaaacagaaa
    13141 catttaattt attttgagga ggagcctatc tatatgaaaa agaaagtctc tataggagaa
    13201 agaagtttta cacttttcaa caatacgttt ctaattttgc tagctttgat ctgtatcgtt
    13261 ccttttttaa atatcatcgc tacatctttt gcttctacac aagaagtcgt cgcgaaaaag
    13321 tttatccttt ttcctactac cttttcttta gacgcgtatc gctacattct ttctacgccg
    13381 accattttta gagcacttgc tgtttcgatt ggtgttacag gtgtcggaac gatcgtcagt
    13441 atgtgtgcaa cttcactgat ggcctacggt ctttccagaa agtatttgtt tggtcgaggt
    13501 ttcgtcaact ttcttgttgt attttctatg ttgttcagcg gggggatgat tccaacgttc
    13561 ttagttgttc gttctctagg gctggtcaat tcttactggg ccatgatcct gcctgtggct
    13621 gttaatgcaa tgaatatgat cattatgaga aactttttcc aagccttgcc ggatagtttg
    13681 gaagaatcag caaaaatgga tggttgcaca gattttggtg tatttttcaa aattatgctg
    13741 ccattagcgt taccgtctat tgctactatc tcgctttttt atgctgttac ttactggaac
    13801 acttatatga ctgctatttt gtatatcaac gattcatcta aatggccgat acagattctt
    13861 ttacgccaaa tcgtcattgt atcaagtggg atgcaagcag aaagtagtgc cgttgatgtg
    13921 attccaccag cacaaacagt taaaatggct gttatcgtta ttgcaacagt gcctatgctg
    13981 attgtttatc cttttgttca gaaatatttt gtcaaaggag cactcgtagg ttctgtcaaa
    14041 gggtgattat gtactgaacg caaaatggta gttgtgtcag aagtggtcag cttcaagaaa
    14101 taagaaggca tttacgaaaa ttgcttctta aatttttgtg aaatttcggc ttatttttcg
    14161 aagaatctac tcctggaaca ccgtgtatca gaggttgtga aagagacgtt cagccttgag
    14221 cgatgataaa aactctcaca aacattgcga cgagtaaccc acaggagcaa taagaagcga
    14281 ttttgaaaaa tagcttccca tatttcacaa aattatgcct tattgtcgat agcctgtctc
    14341 ttgaacgcca tttattcgta aaaaagaact gtgacaagac ttttgtcaca gttctttttg
    14401 cgtgcttagc cttgaaattt aactatccat aaatacaaat actagggtta ttctaacttc
    14461 ttttattgct tgaatcagtc cacaagaaaa acagcctctt gattcacaaa tatttttttc
    14521 gatattctcc cggcgtaatc gattccttct ttttaaagaa acgaataaaa ttttgcggat
    14581 ttcgatattg caatctttct gcaatctctt ttacggagag gttagtctct ttcaaccatt
    14641 tttttgctac ctctaaacga tgattttgaa caaaatcacc aaatttttct cctgtttcct
    14701 ttttaaatac attacttaaa tagttagggt tataatgcaa cctatcagcg atcacatcta
    14761 aagacagatc acgatcatat tctgtgtaga cgatatgcat aatattctcc gacaaatttt
    14821 taaattcatg atcagtgatt gcttgagagc attctgtgat tggcagaact aattgttcta
    14881 aaacaagttt ttctagttct ttgggatgat aattgttcag tgcttttaca taaatttgtt
    14941 tcatgttatc aaagtttttt gaatctactc ccaataactg tcctaattga acgagttcat
    15001 tgatcaagcg gaccgaaact acttcccgac tcaaaggatt tttattttgt gcaaacaatg
    15061 catcaatcaa ttcatgtact aaattcttca cttcctgatt tccagaacga atggcttcaa
    15121 acaatcgatt ttgttcttct atcggatatc taataagtgt tttttcatga gcagcaggaa
    15181 cgatttcctg ataaaaaata attgaacttg gaccagtatt tacacgataa tacagggctt
    15241 cttttgcccg atcaaccgat tgcttgcttt cattcagcgt ttcgaaccgg ttactgatgc
    15301 ctatactgat agttaaattc aagtattctt ttacacgttt ttgaatgagt cggcaatact
    15361 ccatgacttt tttgcttgct tctgcatcat ctttatccat tcgataaata gtggcttgca
    15421 tttcttcatt caaaacgatc ggcagcattc gattttcctt tggcacaatt tccgtgatca
    15481 tgttgttgat cgctaaaagt agcaaatcac gctccccacc gtgactatct tctaacagat
    15541 cgatttgaac caaagctgta taatagcagt cattttgaga actataacca aattgctgca
    15601 accgctgatc tagttctcgc ctttctactc gatttctaaa taaactaagt acaaatagcg
    15661 tttcaagctg tggcttttgg gtaactaagt gtgcacttaa cgcttctttt tctcctatga
    15721 tccgactgac tgattgagcg accaaatcta gttctttccc actgaatcct tttgatttaa
    15781 gattcagacg ttcctgtatt tgagaaatcg gacgggcaaa tctgtccgaa aataagtagg
    15841 aaagaagacc aatgagtcct accagtgtta aagacactgc aactagcccg attctcagat
    15901 tgcggatcat tgcgccaatt gcactagagt caacttctaa tctgtaaatc caattattat
    15961 aatctgattt cagtactata tacgttttat ctttttctct tatcactttc agctgattgt
    16021 aatctagatc cacgtccgtt aatacagttg gttgctcttt attttttact gtctgagtag
    16081 taaaaaggtt gccaacttcg ttttcaatag aaagtatgtc ttctccactt tcaccaacaa
    16141 cttgttctat tgtatgtttc tctatcgtag caattcccaa tgcatatttt tctttttcaa
    16201 acattggcaa agaaatcacc atttcgattc ctttgttttt cttttgccaa aataaattat
    16261 ttttgttatt tatgtacttc tgttgatact tttcaatttc ctcttcggtc agttgactca
    16321 acgatccatt gattactccc catttaccag ccaaactgac taaatcataa ttattatcat
    16381 ccataacaat cgtttctata aaatttagtt ctttcctgat ttcagaataa gtggagtaat
    16441 ctgtatatgt cagcggatgc ttgaatatgt ccgagaaatt tttggtcgaa ccatacgtag
    16501 aaaacgaata ttcaatcgtt ctaattttcc attcaacgtt agttttaact tgcctgagca
    16561 aattcttttt gtctgttaaa taactcgatt cgatcgattc ataggtattc tggtaaatga
    16621 ataaactaaa tcctatgaca agtacggctc ctaataaaaa catcggtaaa aatattttat
    16681 aaaatactgt acttttcttc attttcttcc tctcctcctt ttaaaataaa aggctttcat
    16741 ttttctgtaa aattgatact catctaatta aatgagcatt ccccgctttc aagaatagca
    16801 taattaaaaa taaaaatcca agtaattttt aacgtgtgca atcttttcat atcaaagatc
    16861 tattgactta tcgtagttat cgtaatatga tgaatgcgtt aacaaaaagt gaaaaagtaa
    16921 ggaaaatgat ggaatggtaa cgatcaaaga tgtcgctaaa gcagctggcg tttctcatac
    16981 gacagtttct cgtgctttga ataacagcag tttaataaaa aatgaaacaa aacaaaaaat
    17041 acgacaacta gctaaggaaa tgaattattc gccaaactat agtgcaaaag gtttagtgaa
    17101 tcaaaaaaaa tatctaatcg gactattttt ctcaagtttg cataaaggaa cttcatcaaa
    17161 tttcctagca gattccattg caggcatcca ttcagtatta gacacaaatt acagtttatc
    17221 tgttgaaagt atcgatgttg ttgatttaac agaaatcaac ctgcaacgct acgatggact
    17281 gatcgttatg agtcaaagtg atgatgatca gcctttcatc gactatctca aagaaaaaga
    17341 atttcctttc gttgttgtca atcgtcacat caatgatcca gcggttatca acgtcactgc
    17401 cgatgatgcg gccggtgtta tgcaggcagt agattacggt attggatgcg gacatacgaa
    17461 gatcgcttat attggcggaa agaaaaattt tcgttcgaca aaagaacggg aaaaaggggt
    17521 attgaaaagt ctaaaaaaag cgagcatccc tgtcaatcca gcctattttt taacaggaga
    17581 ttacagctta gaaagcggcc tccaaaacat ggaataccta ttatcacttt ctgacctgcc
    17641 tactctggtt ttttgcggca acgacgacat agcgattggt gcattgagag ctgtacattt
    17701 agctggcctc cgtgttcccc agaacatttc tctgatcggg ttcgatgatt ctccagtcgt
    17761 ctcctatctc aatccgccgc ttaccaccgt acgtaagcct ttgaaagaaa tgtgtcaaga
    17821 aggaacaaag cttttactag atttgatcga aggcaggacg atcgaacaat ctaaaattga
    17881 gctctccacc ttgttgatcc ttcgtgagtc gatcaattac ttgtaaacta caagtaattt
    17941 tatataaaaa tgttaacgtg tgcataacac agaaaggaag tttatatgaa aaaactgaca
    18001 aaagaattag tgaaaaaaaa cgaagcacct gtaaaagttc ttcagtttgg tgaaggcaat
    18061 tttatgcgtg gatttattga ttggcagatt cagcagctga ataaccaaca attgtttaaa
    18121 ggtaatgttg caatcattca acctttagaa caaggtcttg gaaatatgat gaaccagcaa
    18181 gataatttat atacggtcat tttacaagga ctcagcaatg gcgaaatcgt tgatacttcc
    18241 gagattatca ccgtagtaga acaaatgatc aatccttatg atgaatggga tgcttatcta
    18301 tctctagcag aaaatgacga cttggcctat atcgtctcca atacaacaga ggcaggaatc
    18361 caattcgtgg agactgataa tttaacagat actcccccac agagcctccc gggcaaactg
    18421 acagcctttt tataccgacg tttcaaatta aacaaagctg gctttaccat cattccttgc
    18481 gaactgattg acagaaacgg cgaacgtcta aaagaaatca ttttacgcta cgcagaaaaa
    18541 tgggatttag aacctgcttt tgttgagtgg ttagaaaaag aaaatgtttt ctgttgtagt
    18601 ttagttgatc gcatcgtccc aggttatcct cgcgaagatg caggtgtttt caatcaacgg
    18661 cataactatg aagatcaatt gatggtcaaa gcagaaccat tcatgctttg ggtgatcgaa
    18721 gggcctaaag aattggagaa tcaactacca ttcaaacagg ctggcttgaa tgtcatcatc
    18781 acagatgata tgactcctta tcgtgaacgt aaggtgcatt tgcttaatgg accgcataca
    18841 gcgatggtcc ctcttgcttt gttggcggaa ttggaaactg tagaagaagt aatgaaagat
    18901 cctttattct ccgcatatgt tgatcagtta ttcaatctag aattgattcc tatgctttct
    18961 ttacctaaag atgaattagc catttatgct gaccaaatca aagaacgttt cttgaatcct
    19021 tttgcccatc acaaattaga agcgatttca ttaaatagtg tttcaaagtt cagcacccgt
    19081 ctgcttccag tgttcaagaa gtatatcgaa gaacaaaatc aggtaccacc gttaatcact
    19141 gtttcactag ctgcgctact tcttatgtat cgaggcgatc aagtaaagcc acatgatgat
    19201 gaaaagacga taagcgaatt tactgatgct tggtctgata atggaacagc tattcctcga
    19261 ttattgcaaa acgctgcgct ttggggagaa gatttgtctc aaataccgaa tgtgacagat
    19321 acagttcaag aaatagctgc aaagatcgat caaaaaggtg tccggtcagt attacaagaa
    19381 atgaaaggaa cgatggtcca tgcttgaaac cctgcaacca acaatgaaat taaatcgacg
    19441 agacagtgtg gtcgtcgcat taacccctat tccaaaacaa acacaactga cgattgatca
    19501 gcaaacgatc accactcttg aagatatccc gcaaggtcat aagattgcat tgggtgattt
    19561 gaaagctgga gacaacgtga tcaaatatgg ctatccaatc ggtcatgtga cgacagacgt
    19621 aactgccggt caatggctgc acactcataa tgtaaaaacc aatctttctg gtgaattgga
    19681 ttatcgatat gaaaaagatg tccatccaag tcattacctt tttgagaacc gaacatttca
    19741 aggttacttg cgaaaaaatg gaaaagtagg gattcgtaat gatttgtttt tagtacctac
    19801 cgttgggtgt gtgaatggca ttgctgaact gatcgtcaaa cagttcaagg aaaaacatcc
    19861 agatctggga cagtttgacc atattacgat tttaaagcat ccttatggct gttcgcagct
    19921 tggaaaagat catcaaaata ctcgagaaat tttagcggac gcagtaaatc atcccaatgc
    19981 cggaggagtt ttagtatttg gattaggttg tgagaacaat acagttcctg aattcaaaaa
    20041 aattttaggt gaatatgatg aagaacgagt gaaatttcta gtggctcaag atgtttacga
    20101 tgagatcgaa caaggtgtgg ctctattaga agagctttta acagcggcgg aaaatgacca
    20161 gcgcatatca gtcccgctct ctaagttgaa tgtcggtttg aaatgcggcg gttctgatgg
    20221 tctatcggga ataactgcta atcctttact tggtgcattt tctgactact tgattgctca
    20281 aggaggcagc acgatcttaa cagaagtgcc agaaatgttt ggcgcagaac aagtattgat
    20341 ggcacgagcg gaaaatgaag aagtttttga ctcaatcgtt gacttgatca atgacttcaa
    20401 acagtatttc ttgtcttacg gggaaccagt ttacgaaaac ccatctcctg gtaataaagc
    20461 aggtgggatc actacattag aagataagtc tttaggctgc acgcaaaaat caggaagctc
    20521 tcctgtcgtt gatgtgctac aatatggcga aaaaatcaga aaaccagggc ttagtttatt
    20581 gcaagctccc gggaatgatc ttgtagctgc ctctgctctt gcttcatccg attgccagtt
    20641 ggtcttgttt accacaggaa gagggactcc gtttggctca tatgtcccaa cggtcaaggt
    20701 atctacgaat acaactcttt ttgaacgcaa aggtcattgg atggatttta atgcaggcgt
    20761 cttattagaa caaccaatgg aagtaatctt agaatcgttt gttcaaaaaa tcatcgctgt
    20821 agctagtgga gaagaaacga aaaatgaaca aaatgacgtt cgagaaattg ccatttttaa
    20881 aaacggagta acactttgat tttcataaaa cagtataaaa agaaagaagg aagatttatg
    20941 tttttatctg atgatttttt attaaaagac gattgggcga aaaagcttta ccattcctac
    21001 gcaaaaaaca tgcccattat tgattatcac tgtcacttag atccaaaaga aatttacgaa
    21061 aacaacaatt tcagcaatct gacagaagct tggttaggag gcgatcatta caaatggcgc
    21121 ttgatgcgag cttgcggcgt gcctgaagaa aaaatcacag gaaatgcttc tgattttgaa
    21181 aaatttttag cttggtgtca aaccgtacca aaaattatcg gaaatccgct ttacagttgg
    21241 tcacatcttg aattgaagcg tttcttcggt attgacttgt tgataaatga agaaaatgca
    21301 gagaaaattt ggacacaagc aaatgaaatt ttggcagctc ctgattttcg ccgtcgggaa
    21361 atcgcaaaaa aatcgaatgt agaagtgatt tgtacaacag atgatcccgc agacgacttg
    21421 cactatcatc agttgcttgc tcatgaagaa ccagatttga aggttctacc ttcattccgt
    21481 ccagataaag cactcaatat tgaaaaagaa ggatttaatg catgggtcac actactggaa
    21541 gaagcagctg accaaacgat caccacctat caagaactga tagaagtctt agggcagcgg
    21601 atagaatact tccatcaaca tggatgtcgt gtttccgacc acgctttaga catcttacgc
    21661 tataaagaag cggatgaaac agtattagaa gagatttttc aaaaagcacg agctaacaaa
    21721 ctattatctt cagatgaaat cgatgcttat cgtacagaaa cactgataca tttgacccac
    21781 ttctatcatt ctcataattg gacgatgcag ctgcacatcc atgcttatcg taactgtaat
    21841 acacaaatgt tggaaaaatt agggcccgat accggctatg atggaatgaa tgatctttct
    21901 ttgactattc ctctgcaaaa attattgaac cgtgcagaag aaacggatca gttgccaaag
    21961 acgatccttt attcattgaa tccgaatgac tatcctgcga ttttagcttt gatgggctgt
    22021 ttccaaaaag aacagaatgg aaaactgcag ttaggttctg gatggtggta caacgatact
    22081 cgagctggta tgcgcgatca attgacacaa tttgctgatg gtagtgcttt ggggaatttt
    22141 gtcggcatgc tgacagattc aagaagtttc ctgtcctaca ctagacatga atatttccgt
    22201 cgtgttcttt gtgaattttt aggcgaaatg gtcatgcgag gagaagcacc tgaagatgaa
    22261 caacttttag gaagtctagt tcaagcgatt agttatacaa atgctaaaaa ttactttggc
    22321 ttttttgcct aattgattaa tagtaacagt aacgaatcaa aaacaggata gtgaaataac
    22381 ggaatccgtt tatccacgtt ttagagaaga agacggacca aatttgctcc gtcttcttct
    22441 cttgctgttc tgcttcattg cctataaacc tttatgaaag aagagataac atgcctacta
    22501 tccatattgc cggtgattct actgcggcag aaaaagatga aaacaagcgt cccgagacag
    22561 gttggggaga aaaaatctct gcttattttt cacccgagat ccagatcaat aaccaagcaa
    22621 agaatggacg cagcaccaaa tcatttatcc aggaaggaag gttagcccgt ctagcttcac
    22681 agtttcagcc gaatgatttc ttatttgtac aatttggaca taacgaccag aaaatccaag
    22741 aagaaagagg tacgcttccc ttcggtagct atttaaaaaa tctgcagata ttttttgaat
    22801 gcgcaaaaaa agcgaacgtc caaatggtct tactgacatc tgtgacgcga cgagattact
    22861 tagaaaacgg aacattgaat ccagatatac ttggagacta tccaaaagcg atgcgtgcat
    22921 ttgctgagaa acaccatatt cctcttttag atgtgttctc aagatcacaa gaattgttcc
    22981 agaccttctc aaaagaagag acaaaaaaat tctacctgca tcttatacct gatacttgta
    23041 aaaattaccc agaaggacta aaagacaata cccactttag tccagaaggc gctgataaag
    23101 ttgcccgaat aattgttgaa ttgataaaag aatcccgtct gcctcttgct aattatttac
    23161 aaaaaggagt ttgagatgtt tccattaaat ttaggcatcc gtgcccacga tttagatgtt
    23221 cgaaatcgtg aagaaatcgt tcaaaaagta tcacagcttc acttgtccca tatccaattt
    23281 gcaccacaaa aagcatttcc tgaacaactt aaattaactg acatgacact tgggagtgct
    23341 gcctattttg gtgaatactt ccaaaaaaac aatatcgaac tttccatttt aggatgctat
    23401 atcaacctat ccagcaaaaa tcaagaagtt cgtgcacaag cattggcaac tttcaaacag
    23461 caaattgctc tgtgctctgc ctatcaagcc aagatgattg ctacggaaac cggtagtgtc
    23521 tctgtcggtt acacaaaaga aaattttact gaagaagctt atcagatcgc cagaaattct
    23581 atcattgaat tagtcgctca tgcagaaaat tttggcgtaa ctgttgctgt tgaagcaggg
    23641 atcaatcatc caatctataa ctatcagtta gctaaacgtt taatcgatga agtagcttca
    23701 ccaaatttaa aaatcatttt agattgtgcg aatttgatgc ataaagaaaa tcatgctgat
    23761 caggaaaaag tgatccgagg tgccttggac aatttacatg gttatattac agcgcttcat
    23821 ctcaaagact atatcatgga aaatggaaag atccgcatgg tacctgttgg caagggctgt
    23881 ttagatttcc gcccggtact gcattatatc aaatatgagc gtcctctaat gtacgccact
    23941 cttgaagcta ctcctgagat ttatgttcct gaagcaatag aacacttgca gtttatttac
    24001 caagattgtt aaggagaaga ttatggagga acgaatacag aaaagttatc aaaaatatca
    24061 tgcaaatgct tttcagacat tgctgcatct ttatcaggga aattattggc gttttgccat
    24121 gtcaggactc ttttttatta tcaaacatct tccatcttgg ttaatgccga tcgctattgc
    24181 caatgtaatc aatgcagctt catcgaaaga ttcttctcag ttgcaggcca tttatttcaa
    24241 cgcttttttg atggcttgtc tgattggtca aaatatattg acgaattatt ttcatgtaaa
    24301 ataccatagc acttcgatcc gtcaagtaga agctggacta agagcgaccc ttatcaagaa
    24361 aatccaagaa ctatctattc cttatcaaaa agaattgcaa tctggacgaa tccagtcaaa
    24421 gatcatccgt gatgtagaag ctatccaaac gttgtcctct caagtatttg tcagcggatt
    24481 aaatatcgct gtcaatttat tcgtagcttt aggtatcacg ctttataaaa gtcctgttgt
    24541 tttcatgttt ttcttattga ctgttcctct cgccgcattc gttactctct ttttccgtaa
    24601 aaggatcaga cagtccaact cagacttcag gcaagaaatg gaagagactt ctgcaaaagt
    24661 gatcgaagtg atcgaattag caccgattgc tcgagctcat gggttagaaa aacacgaaac
    24721 aactcgctta aataagcatt ttactcaaat ctatgaaaaa ggattccact tagatatcct
    24781 gcaatctgtt ttcggatcag tcagctgggc tagttttcag atatttcagc tgatttgttt
    24841 gattttcact gggatattag ctttacaagg gcgcatccaa cccggcgatg tcgtgatgta
    24901 tcagacgtat ttcacgacga tcgtcaattc cgtttctgga atgatcacgc tgattcctac
    24961 gatttcaaaa ggcatggaat ctatcacttc tattggcgaa ctgttgattg ctggcgatgt
    25021 agaaacttat cacggtaaga aaaaaatcca acatttggat ggaaattttc gttttgagga
    25081 tgtttctttt ctttatccgg actcactcaa accgattatc gatcattttt ctctagaggt
    25141 gaaagctgga gaaacaattg cttttgttgg gccatctggc tctggaaaat ctacactcct
    25201 taacttattg attggattta tccagccgac ttcaggaaaa atttttctag atgatcagcc
    25261 tttggatgaa ctggatatgc gaagtgtgcg aaattattta gccgttgttc ctcaaaccac
    25321 tttgttgttc tccgcttcga tcaaagagaa catcacttat ggcttgaaaa acgtatcaaa
    25381 agaacgttta gatgaagtca tcgaagctgc gcaactttct tcactgattg accagcttcc
    25441 tgatggtctg gataccttag taggcgaaca tggcaacaag ctgtctggtg gccaaaagca
    25501 acgtatctcc attgcacgag cgttgatacg tcagccaaaa atcattttgc tagatgaagc
    25561 aacctcagct ttagataacc agtcagagaa aaaaatccag caagccctga attacctgac
    25621 agaaacaccc actactttta ttgtggctca tcggctttca acgatcaaag aggcagataa
    25681 aatcgttgtg atcgatgaag gaaaaatcgt tgaaataggt acttacgaaa aactgttagc
    25741 tcaaaaaggc tatttctacc gcttgaaaaa tgaatgacaa aaaataggag ctatgacaag
    25801 acttttgtcg tagctcctat ttctgaataa acggtgttct cctgccaact cctcggtcac
    25861 aagtcacaat agtcatcaaa catgagaagc tgttttttga aattgttcct tgtaactcag
    25921 agttaaacgg taggctcacg acctcttggt aaatggtgtt caagagacag cctatcggca
    25981 ataagaagca attttgtcat cgctcaaggc cgaacgtctc tttcacaccc tctgttgttt
    26041 cataaaatag ttttattcgc tgtaaaggtc gcgaactggt gtcatgatga tacggttcaa
    26101 gtcattgatg attgtactga agccttgctc tttttgcatc aagtcattga ttacttcttc
    26161 tgattggatt ttcacagcca tttcttgtgc tttcattgca tcttcgtcgc tgaactcttg
    26221 tccactcatt tgtttttctt gtaattcgat ttgcatcgct tggaagtctt tgaacagttt
    26281 gtgtgcttct tcgtttgctt gtactttctc gtacgcttct ttcaacgctt tgaattctgg
    26341 taattcacgg atctcacgtt cgatttgatt cgcgctatcg tagatgtttg acatgttatg
    26401 ccctcttttc ttttagtagt tactctctat tgtaacatgg ggttattcgt tttcaaaggg
    26461 gctactctcc ttgatatttc cgccagaggt ctttagccgc ttcaccaatt ccctgaacgc
    26521 cttctttcac catatcgccg aagtttttca gcccttcttt tgcttgttca ccatattgat
    26581 tcaagttatc ttgccaattt ccagactctc cattagaatt tcctgtgttt ccaacttgat
    26641 ctgcagctac cacttgtcct cctgtggcat aggcatctgc tacactaaat cctgtttttg
    26701 aagtatacgg aaggatgcct tctgccgctg atttgaagac tttcccgaca acatttccac
    26761 ttgtcccttc caaatagtgc agcttgctgg attcttcgta acccatccat gtcgaaatca
    26821 ctacgtctgg cgtgtaaccg atcatccatt ggtcatttgc ttttgtggca tcgaaattag
    26881 tttctgtggt tcctgttttc cctgctatcg tataacctgc aggattcgca gcagccgctg
    26941 ttccatttga aaatgtacca agaagcatgc tggtcatttc atcggctgtt tcctttgata
    27001 tgacctgttt tggttttttg tttgtgttat cgacgatcac cgctccagtc gaatcaacga
    27061 ttttcgtgat aagatgcggc gtatacatct gaccatcatt ggcaaatgca ctgtaagcac
    27121 ccgccatgat caatggagat actccttttt ccagtccacc aagggctaag ccataatact
    27181 tatcagattt tgctaaaggc aggccgaatt cttctgcttt atcatatcct ttttgtaaac
    27241 cgatctcatg caaaagccat acagtaggca aattcaaact ttgtgcaaca gcttgataca
    27301 tcggtacttc tccttggtat gtcccgtcaa aattatgcgc atcgtaataa ctttgcggtt
    27361 cgtctttcaa gatactggaa ggcttgtatc cagcttccag tgctggtgta tatacgctta
    27421 atggtttgat cgttgatcct ggtgaacgac gcatctgtgt agcaaagctg aatcctctaa
    27481 agacatgatc tcctcttcgt cctactagtg cttgtacacc acctgtattc ggatctaaag
    27541 ctactgaagc agattggacc atggcaccat ctgaagcatt tgctgggaat aatgcactgt
    27601 tttgataagt agcttctaat tgcttttgat aatcctgatc taaagctgta tagattttat
    27661 agcctttatt catgacatct tcttcatcaa gaccatactc gttaactgct tcatcgatca
    27721 ctgcatcaaa ataataagga taacgataac cagaatctga attatcgtat gtgtcattaa
    27781 gatacgcgct gatatctgtc tggctttctg cttgttcttg ttcttttgtg atttttccgt
    27841 tttcttccat tacacttagt actgtgtttt tccgatcatt cgcatttttt gggtgatcga
    27901 tcggattata aataccagga cctttcagca ttcctgccaa agtagcagcc tcagacagcg
    27961 taacattcgt tgcatctact ccaaaatact ttctggaagc gtcttggaca ccccataccc
    28021 cgttgccaaa ataggcattg ttaagataca tggtcaagat ttcttctttg ctgtacttct
    28081 tttcgatctc gattgccaaa aataattcct tcgcttttcg atcaaacgtt tggtctaatg
    28141 tcagatacgc atttttggca agctgctgag tgatcgtact tcctccgccc cctgatgttc
    28201 cgccagacaa gaccattcgg actgctgcac gggcaatccc tttgatatca aacccgtgat
    28261 gctcgtaaaa attcctatct tctgtcgaga ttactgcatc ttggatatag ggagaaatcg
    28321 cgtttaattc tacatatgtt cctttttggg cgtacagtgt gccagcttct tcgtttttct
    28381 tatcataaat gactgtcgat tggcttaatc ctgattttaa cgtttcgaca tttgcttgtt
    28441 ttgctaaaat aaagaggtag atacttgtca ctaagacaca gatcaaacca atcagcaaaa
    28501 tcaatttgtt cacatgatat tttttccaga tgcgctttcg ccatcgatgg aattgtccga
    28561 gatatggtct gatccaacgc caaaaactga tcaaagcagc ttttatcttt tctaaaatgc
    28621 tttgaaaatc catttgcttc ttctcctgtc tatttgattc aatcgtctta ttttaccata
    28681 gtgaaagagt gttttcttcc tgcttaagtt gtagttcaat atgttttttt acgttagtgc
    28741 aagtagagta acataagaat gaacagaaaa atgtaagttt tttcttaaat atgttttaaa
    28801 aatggtacaa ttagtctgcg attgcactga aaggagtaca cttgtgaaaa tcacgatcac
    28861 tttacctatg aaccatcctg aaacaacaat tcgcgacttg cttgaaaagg aatggttagt
    28921 tcctagaaag gttcgacact ttttgcgtat acgtaaaaac gtttggatca atcaacaacc
    28981 tgctttgttc catcatgaag taaagggcaa tgaccagatc acattgttat tcgaagaaac
    29041 agattataat tatcaagaag ttcaattagg agatgcgaag cacatccgtg ttttatatga
    29101 agatgagcat ttgattatag tggataagcc tgcaggaatg aagactcatc caaatgaacc
    29161 gacagaaaac agtaccttac tcaatcattt agctgcttat ctcgacacta aacagcaacg
    29221 accttacgtt gtccaccgct tggacaaaga aacaagcggt gccgttttat ttgcaaaaaa
    29281 tccatttgtc ttgccgatac ttggaagaat gttggaacaa aaaatgatct atcggagata
    29341 tcaagccatc gtatggggag aaattgccaa agatatgacc atcaccaata aaattggacg
    29401 agatcgccat gatcggagaa aaagggtcgt agatgataaa aatggtcaga gcgccttgac
    29461 acatgtcact gtcaacaaag tactttctga acaaacacaa gtctattgtg ttttggaaac
    29521 cggacgtaca caccagatca gagttcattt agctcataag ggacatccga tcgttggaga
    29581 ccctctctac caaaatagaa cagctgaacg actgatgctc catgcattag aactgcacat
    29641 gatccatcca tttactcaag aaaaaatagt cggtaaagct ttacctggat tatgggaata
    29701 gaaataaaaa agaacagagt cttatcagca gataatgata agactctgtt ctttttgtgt
    29761 aatttgtctg cttacttttg atgttcgtgt tctgctaaca gcaaactacc gataatccca
    29821 ctttcattcg gcagtcccca acgaacgata tattcactta cttgaggtgt ttccatataa
    29881 ccacccatca attcaacaaa ttgctgacgg attttttgaa ggagatgatc ctgattcatt
    29941 accccaccgc ctaaaataat cttttcaggt gctaacgtca acgtatagtt gatcagtgct
    30001 tgagcaatat aataagcctg aatagcccaa acaggatgat cttgcggcag atcttgccct
    30061 ttgatccctg tgcgtgcctc aatagacggt cctgctgcta atccttctaa acaatcttta
    30121 tgataaggac aagtaccttc ataatggtca tctggatggc gtttcaccca aatatgtccc
    30181 atttcaggat gtgcgatccc tgaaaaaatt tcttcattga tcacgacacc tgcaccgatt
    30241 cctgttccca cggtaaggta aatacaacta ttttttcctt gtgctgctcc ttgcttcaat
    30301 tccccatatg cagcagcatt gacatccgtt gtccaagcag caggaatatt gtagcgttcc
    30361 ttgattgtgc caagaaaatc gaattgtgcc cacccttttt tgggagttgc caaaacgtat
    30421 ccgtaattat cttttgtttt gtcgatacca atcgggccga atgagccaat cccgattccc
    30481 ttcaattcat aacgatcaaa aaattcaaat acctgatcca atgtttcttc tggtgttgtg
    30541 gtagggatac ttaatttttc taaaatttct ccaccatctg aaaccgcaca cacaaatttt
    30601 gttccgcctg cttctattcc gccatacatt cgttttcctc ctcgaatccg attgtccgaa
    30661 tctcataagg ttggattatt gggacaaccg cttgcttcgt tggttcttca agcagattca
    30721 atatgacagt ttttccattg tctttagtta tttccaattt ttctaaatga ctagacatat
    30781 tgaaacctct tactactact ttatcactaa acttgctgcg tttcaaagct gtaattgcaa
    30841 acgtttcact ttttatcgtt aaatattgtt gttttgaaat caatgttcct tcatgatgtt
    30901 tgatttgttg agtggaaaaa ggaatctgtg ctgcgtatgc atgcaaataa gtagagaatt
    30961 tttcttctgg tccatgtagt tcaatgctgt aattgaaccg atgctcacct agacactgtg
    31021 cttctggtgt tggaaaatat ccccaatcgc ctagttctcc cacactgcga agtagcgtca
    31081 ctgcgatttg tccgtcttct gttacttcat attcattcaa accaaagttt ccgactgtta
    31141 caccgtactc ttcatcatga atattgacaa atgcttgctg atgttgcgga ttcgtggggt
    31201 tttcccaact tttagacaca tggtttgggc gcttcaccac ttcaaaaata ctatctgctt
    31261 cgtgatgttc cacatgtaac tttgtaggaa acaagacacg taagcgatga tctttcatct
    31321 gattgtcgag agtcgtttca aagtcgattt ttttactatc ttttctgatt gtcatgattg
    31381 tttttatttg aaggattcgt ttttctttgg atcgttctgc ttttcgataa cgaaattcca
    31441 caaccatctg ctgttctttt tctaaacgct cgtctgcaga aacagggatt tccatttcct
    31501 gtagaatact tatttttgct atttccggtg tatctactaa tacttttact tcagagtttt
    31561 ctttgttact ggataggatt ggctgatcac agaaaggttg tttaaagata tactcatttc
    31621 ctatatcgcc gatatcttca aaaatatgca gattattcaa ctttcgtttg tttttgcgat
    31681 cttccattgt aatggtacca tttttttcaa tcgtcaaatg tagagggccg ttttcgatga
    31741 ttcgtccact ctgatgaatc attgtttcct tttcttctgc tttcgcttcc ccttctacaa
    31801 gcgcgaatgt ttcccaagaa aatgctggca tgttttctaa tggtaaagtc actttgatca
    31861 ttcttgccat atatggcaca cggaaacggt ctttcggcaa atcataacca aattggacag
    31921 tttcttcaga taaaatagct ggtatagtgg ccccactttt tgtttctaca tgatacttac
    31981 gtttaggcaa attttctaac tcttgatata gttgttcagg tattccttca gcgaatcttt
    32041 ttctttctaa ttcgatggtt atttctgctt caccagtttt atccattcct gcagtattta
    32101 caatgacaaa tggaaagctg tcttttggga atccggtcgt atcgattgct gcggtcagct
    32161 gttctaagga atcttgtgca agatactttc caacttcatc tgctttttca aaacgaggta
    32221 tcatttcccg atggacagaa tcgactgaac aaccgcaaat actgtcgtga ggatgatttt
    32281 gcattaatgt cttccaagca taatctaatt gatcatgggg atagtttcca ctaacttcat
    32341 aagccatcgt cgcaagtggt tctgtaatat tttctagctg tcgctggacc ttggtattcc
    32401 attgttttaa gtaaacccga gcagatgctg tattggctaa agtataccag ccatccgttt
    32461 cttgacttgt caattctcct tctactgagc ctaagtcttc tggcacatct ttttgaactg
    32521 cttccagata gtcagtaaaa ttagaatgaa taaactcgta atctggaaac agttcatttg
    32581 cgagatggat tgcttttgat atatcttttt gtaccggttg atgatcaaca ccattcatca
    32641 tcaataagtg gttcgtagag gcatattgtt ctgcatccgc taatttttgc ttccaaaagg
    32701 caagagctgc ttctttttct actggaatct cattcccgtt gctataccaa ttggcaaata
    32761 gcagaccaaa gattgctgtt tgatcaggtc ctttccacca catctctgag tattgggaag
    32821 aatagttttc tgcttccaaa acttgattat caaagccaat tggttttaca ccacggccaa
    32881 acgctgctgc tgaaatacca gcttgtttca ttaattgagg cgtctgtccc atatttccaa
    32941 atgtatcggg aaaatagcct aacatgacag gggttcccca tttacggctt tcttccatgc
    33001 caatcagcat atttctcacg ttcgattccg agctgataag aaaatcatct tgcaagatat
    33061 aaaatggtcc aattctcaat tttccttcat tgattgcttg ttggattgcc tgtctttttt
    33121 ctggccttac ttgaagatag tcatctaaaa taatcgtttg cccatccaaa tgaaaactgt
    33181 tgaaggacgg atcaacttca aatagctcta gtagatcgtc tatcaattca atcagtcgca
    33241 tgtgatgctg ttcgtaagcc atgtaccatt cacgatccca gtggctatga gaaatgatat
    33301 atactttctt tttcatttac gatacctcct acttttctac tctaatatca aagtaatcca
    33361 tgactaattc gcaaaacatc atatttgccc aagaaaacca ttctctcgta taattttcag
    33421 gattgttcac atcgaatcct tcgtgcataa ggtgtgttcc tgcgtcagtt gctacaagta
    33481 aattcaatat ccgctctttt tcagatttgt ctgacgttgt cattccttcc atagctaatg
    33541 caatcggcca aacgtatttt tctggcgtat gggaggatcc aattcctttt gcgtactttc
    33601 cttcatagaa atatggattt tctttactca acaatgttct tcttgtttgc agataatctt
    33661 cctcttgttc agaacaatat cccaagtaag gtgcggcgat cagattgggc acattactgt
    33721 catccatgac tgtggcgttt cccattccat ctacttcata tgcataaatc gtttgccctt
    33781 tttgattaac cgttcgtccg tgattctgaa tcccctcttg gatctctttc ttcaatttct
    33841 gtgtttgctg tacaatttct ttatcatcga gtatcgtttg aaaaatttct tccacataac
    33901 ctaacacgac caccgcaaac atattggatg gtaccagata tccataacgg caagtatcat
    33961 cgctaggacg gaaaccagac catgtcattc ctgtaaaagc tgtttttgca cctcgcccgt
    34021 cattgatcag cgtatcttct tttcttgtcg tgtctcgtac aaatgtataa ggagagtgtt
    34081 catgatcttg ctctgttttg aacacgtcca agattttttt gataccttca ataaaagatt
    34141 gattgaattg atcagttttt cctgtatttt tgtaaagaag ataggccaac tgtattgggt
    34201 agcataaaga atcaatttca tattttcttt cccagatcca atcgttcatt tttgtatgat
    34261 ctgtttggtg gccagcaccg ttagcttctt cgttgaaggc attcgcatat ggatcgatat
    34321 tgatataaaa aaattgcttt ttgactaacc cgctgatcat gtcagctaat tcctcatctt
    34381 cttttgcaat cactaaataa ggacgaactt gtgcagtaga gtctcttaac cacatcgctg
    34441 gaatgtctcc cgtcaataaa aaggtggttc cgtcattttg ctttttgact gttgtcaaca
    34501 gagtattggc aaatgctgct gtaaaattct ttgcccagtc cgcatggata tccccacatt
    34561 tttgtgtgat atcttccata aatttctgca ccgattttgg tatatcttta taagccacta
    34621 ataaaacctt ctttctattt gatatatctt taaagattat acttcataca gccaattatg
    34681 tctacgcttt caaaaaatat ctttttattt actttttcat ccattatggt attatttgaa
    34741 tatgatatat cattaagagg tgtttatatg aatcaaccat tgtaccagac aatatttaat
    34801 gatttgcaaa cggctatttt aactggtgaa ttacctgtag acagccaact accaactgaa
    34861 aaagaacttt cacgtaccta taaagtaagc agaatcactt ccaaacgtgc attgactgaa
    34921 ttagagcaat taggacttat ttatcgcgtt cgcgggaaag gcagtttcgt taaatccgct
    34981 tctccggaac agattcttgc ttctcctaca aaaacacgac ggattctgtt tcttcttcca
    35041 tatgtcgctg atttgtctgt tggtgatttt agcaagggat tgaaccctgt gatgcaagat
    35101 aaacagattg atgtcatgat gacgacgctt gactttctac aaaataaaac agcaaaagag
    35161 ctaatcaatg aatttgacgg attgatttat tatgctcttg atacagaaca acatcttgac
    35221 ttactttttg agctttcatt gaaagaattc cctgtcgtca tattagataa aaaaatctat
    35281 gagttacctt ttcctaccgt tctttccgat aatttccaag gcggtgcgct tgctgcctct
    35341 tatttgatcg aacagggaca ttcaaaaatc gcttatttgt ttggtacgca gactcatcca
    35401 caatccgttc gacagcgtta ccttggttac cttgaagcat tggacaaagc tggtttgact
    35461 ttccacactt cactggatga caaaagcgcg acattagatg aattgctttc ttatatcact
    35521 aagtatgaag taactgcttt tgtttgtgaa aacgatgttg tcgctatcca gaccatgaat
    35581 ctcttgagac agcatggtta tcatatccct gaagattttt caattatcgg atttgatgat
    35641 atccaagcag cagctcttgt tgacccgcca ctgacaacca tcgcacaaga ttttgaacag
    35701 ttaggtaagc ttgcgggaga tgctttggtc acttggttag agacaaaaga tcgaccatcc
    35761 gatagtaaat ttccagtttc attcatcaaa agacaatcaa caaaggagaa ataaaatgaa
    35821 tatcggtcaa atagatacta gacatgcaac agcgaatcag ccaaatttct ctaatgggaa
    35881 ctgccagcct tatactggcg ttccttttgg gatgaattat tttgcaccgc aaactacgga
    35941 tcaaaacggc agctggtggt ttcatcctga tgaccgcgtt tttcaaggct accgtcttac
    36001 ccatcaacca agtccttgga tgggtgattt cagtcatatg ctgtttacgc cgatcaatgg
    36061 aaaactacag gagaacaccc ttttccatgc tcaaagttct taccgcccag aagaaagtat
    36121 attttgcccg actcatctgt cgatctgtca attacgttat ggcatctgtt ctacattgat
    36181 acctagcatg tatggcggaa tcttatctat tgattacagc aaaaatgaca gcggtttact
    36241 tttatccttt ccgggacgcc atcagctctt tgttatagat ccttacacag tggaaggaaa
    36301 aatcacacat tttgctggtt cagaagatcc tgatttttct ttttatttta tattgagatt
    36361 tgaatatcct ttaacaattg atgagttagc tgtttccaac aaaaatagcg actcgatccc
    36421 gttgtatttt gaacagacaa aaaaacaaac tatccgattt gggacatctt ttatcagctc
    36481 ggaacaagca gcttataacc tggaactaga acaaaaccaa agcaaagaga cttaccttgc
    36541 tcacagtcaa gaacaatggc agtcctattt tgatcgtata cagctcacac accatgatca
    36601 acaacaattg cggacgtttt atcataattt atatcgggtg tttttatttc cacaaacatt
    36661 ttatgaaatt gatcctagtg ggcaaaaaat ccactatgat acgactagtc gatctataaa
    36721 acctggtgtt ctctatacaa ataatgggtt ttgggatacg tataaaacgg tttatcctct
    36781 ttattctttg attgctgtag aaaaatacga agaaatgctg gaaggttttt tgaacagcta
    36841 tagagaaagt ggctatttgc caaaatggct gtctcctgat gaacgtggac ttatgcctgg
    36901 gacattgatc gatgcggtca ttgcagatgc tgctgtaaaa gatatccgac gagacttgat
    36961 gcctgaattt ctaacggcaa tggaaaaagg tgctacggtt caaagcaaac atccaaatta
    37021 tggacgccaa ggaacagaag attacttgac atatggctat gttcctaatc actaccacga
    37081 atcagtcaat cacacattag actatgctta tagcgatttt tgtattagcc aagttgccaa
    37141 ggtactagaa gaagaggaaa tgacgaagaa atatcagcag caatcattga actatcaaaa
    37201 tatctttgac tatgagtcag gatttatgcg ttcaaaagat aaagatggca agttcagaga
    37261 accattttca gctaatcgtt ggggacaaga ttatgcagaa ggaagtgctt ggcaaagcag
    37321 ctttgctgtt tatcacgatt tttcaggact gatcggaaaa tatggtggca gtgagagata
    37381 taaagaaaaa ctcattgagc tgtgcaatca gcccccaacg tttgacccag gaggatatgg
    37441 ttttgagatc cacgaaatga gtgagatggc cgcgatagaa tttggacaat tagctatttc
    37501 aaaccagcca agttttcact atccttactt gttcagttat ataggacaac ctgaaatggc
    37561 acagccactg cttaaacagt tgatgactca gaccttcgac gattctccaa tgggttaccc
    37621 tggagatgag gacaatggaa gtatggccgc ttggtatatc ttcaatagtc tgggatttta
    37681 tcctgtcacg cctggaaccg gtgaatatgt tatcggtatg ccattaattg acaaggctgt
    37741 tttgcttttg tcaaatggaa aggaattgac gattgaagca acgcctaatc ggccgcagca
    37801 acaatttatc cataaaatca aacggatgga caaagaatat acgaaattat attttaccca
    37861 tcaagatttg ctgaaaggcg gcacgatctc ttaccaatta gggatcatcc ctcaccctaa
    37921 gaaatatcaa gcaaaagatt tgcctttttc cttatcaaac tataattaac aagaggttgt
    37981 gaaaaagctg tcctgcatca agtaataaga acagccaagc aaaaatagat cctcatattt
    38041 ttcgtttggt tggtcttatt accgcagatg caagcttttg aacaccgttt attgagaggt
    38101 tgtgaaatca gcgttctgac ctgagtatta gagagacaaa tggcaaaata gttcctcata
    38161 ttttgccatt ttttgagcta ataccgaggg tcagctgatt gaacaccgtt tagtggagag
    38221 gtcgtgaaaa agctg
//
LOCUS       DACDYI010000020        36408 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.020, whole genome shotgun sequence.
ACCESSION   DACDYI010000020 DACDYI010000000
VERSION     DACDYI010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.020"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            93..1385
                     /locus_tag="IWM52_000984"
     CDS             93..1385
                     /locus_tag="IWM52_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9973255.1"
                     /translation="MFSDISTIVNTIFIFSVELPVIGVLVLVAVFIISKVNLIFLIFS
                     LIMMGAMVLQYNLFQLPLKKTIENTKSVNQSITGELNAHFDQLEIISAYNFENISILK
                     FDSRFKELFNVFKKQNKLLTLGSCLAQVIFQLWIGGIFLIDVILLAKGTISITNFVIV
                     FMYANMVPTLWTTLISIIWKWQDYSVSLNRYYEYYSNNNKVAGTKKINKINSITGQSL
                     NFSYNKRKVLSNISFSTEMNKLFMISGENGTGKTTLMKLFTKQLPVSNNQLFVNGIDI
                     NMIDNESLRRLISIATQIPYIINSSVKENILMGTKNYDKKKFSKLIELLGISKFMTKL
                     GITLDDNIYDEKILLSGGEKQKISLARALIKSASVIILDEPFRNLDNETRDNLRDYIL
                     NTKKEVCYIIITHDHILDNFLDSNDEILYLNKEYDNNE"
     gene            1378..2406
                     /locus_tag="IWM52_000985"
     CDS             1378..2406
                     /locus_tag="IWM52_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAP9973256.1"
                     /translation="MNSKKLQMGEIVRGPSQISIDVTYKCNFRCKHCYNASGGDNCFM
                     DNELTDDELLELTKDICNLKPYNICFCGGETLLRKKIVLSCATLLKEAGIPNIAIVTN
                     GSLVTQDVAKELFKAGINRFQVSLDGKNASSHEALRLYPNSFEKAINAIKLFRELSEE
                     VDVAFCPTSFNYNQLREVYQLVNTLKVKSLRIQPFMLSGRGMQNREIVPTVSQYRKLV
                     SDINYIKRNEDYTKIDWGDPIDHIIRHRTLLKDFSNFVSIRSDGGLMASPYLPIQVGN
                     IKCHSLSKYWRAGLYKVFSLDLVQDIATQVSSIQDMSKELKKGHIKWLNEDIEIDILD
                     EYKHDILI"
     gene            2462..2680
                     /locus_tag="IWM52_000986"
     CDS             2462..2680
                     /locus_tag="IWM52_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973257.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
     gene            2751..3566
                     /locus_tag="IWM52_000987"
     CDS             2751..3566
                     /locus_tag="IWM52_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973258.1"
                     /translation="MKYNNLKPKLLNENIEYIREENYGYLTIVPKNGTELKDNVFNPT
                     VRYILANCTGKNSVKKIANGLKKKFPSLEYEKFYQDVVGTIKFFKHYNIIDIGDYSME
                     EKKIYKIEQNLELELLNESHYEEIYNFITDHPESMYFHEDIYHQGNSKELEVVLRYNL
                     FSFTSEFYFLRHKERIVGMVSIIVNPFQNLSYSIVNYLICEPAYTTTILTTVLENINE
                     VSILEVKKLVIHLLNSTEDESLIPYLKDSHFFKEAVLSKEYREHDVLRYAYIY"
     gene            3630..3965
                     /locus_tag="IWM52_000988"
     CDS             3630..3965
                     /locus_tag="IWM52_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP9973259.1"
                     /translation="MILLILSLVLLVVSNLMKNNPPTKINSFYGFRTKKSMKNEKSWH
                     LAQKLLGDFSKKKVIPVFLMGIVFFSIEIYSLAILKNENFFVSVLMGESLILILILCL
                     IVHEVNKQL"
     gene            4389..4652
                     /locus_tag="IWM52_000989"
     CDS             4389..4652
                     /locus_tag="IWM52_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973260.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLNLIAHYIRNRWSSIPNFNYKK"
     gene            4968..5398
                     /locus_tag="IWM52_000990"
                     /pseudo
     CDS             4968..5398
                     /locus_tag="IWM52_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347687.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            5537..5959
                     /locus_tag="IWM52_000991"
     CDS             5537..5959
                     /locus_tag="IWM52_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973261.1"
                     /translation="MVKEEVLCPHQDFLYLIPANQETTQHYFTFLEKQNELVEALMES
                     QETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHNYELIPEHWQLLGSSKQQ
                     GKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            6104..6349
                     /locus_tag="IWM52_000992"
                     /pseudo
     CDS             6104..6349
                     /locus_tag="IWM52_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6458..7414
                     /locus_tag="IWM52_000993"
     CDS             6458..7414
                     /locus_tag="IWM52_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973262.1"
                     /translation="MLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVP
                     LSEQPDLKRMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSC
                     ERSRLKEQWSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKG
                     NLLEYFQIRRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLE
                     NAGYREGQLFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQS
                     SLPKCQLVYTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(8250..8921)
                     /locus_tag="IWM52_000994"
     CDS             complement(8250..8921)
                     /locus_tag="IWM52_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9973263.1"
                     /translation="MTIKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQ
                     KKQVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERF
                     RWLRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDEQNVVLTTHPK
                     YFKNI"
     gene            9238..10869
                     /locus_tag="IWM52_000995"
     CDS             9238..10869
                     /locus_tag="IWM52_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP9973264.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            10872..11951
                     /gene="uxuA"
                     /locus_tag="IWM52_000996"
     CDS             10872..11951
                     /gene="uxuA"
                     /locus_tag="IWM52_000996"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP9973265.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            12025..13452
                     /gene="uxaC"
                     /locus_tag="IWM52_000997"
     CDS             12025..13452
                     /gene="uxaC"
                     /locus_tag="IWM52_000997"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP9973266.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
     gene            13536..14183
                     /locus_tag="IWM52_000998"
     CDS             13536..14183
                     /locus_tag="IWM52_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP9973267.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
     gene            14312..15688
                     /locus_tag="IWM52_000999"
     CDS             14312..15688
                     /locus_tag="IWM52_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9973268.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIV
                     LMNFINPILMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLV
                     LTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENY
                     NLLNQKHN"
     gene            15699..16493
                     /locus_tag="IWM52_001000"
     CDS             15699..16493
                     /locus_tag="IWM52_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9973269.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDILFKCLSGEYIDIEQKYIDTFWKNLTNK"
     gene            16506..18272
                     /locus_tag="IWM52_001001"
     CDS             16506..18272
                     /locus_tag="IWM52_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAP9973270.1"
                     /translation="MTIKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGS
                     SRDKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKG
                     IISEERLRDALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGISPIDAYCGLKDTFI"
     gene            18300..20108
                     /gene="uidA"
                     /locus_tag="IWM52_001002"
     CDS             18300..20108
                     /gene="uidA"
                     /locus_tag="IWM52_001002"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAP9973271.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNG
                     KLINQHKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKK
                     ENLVNFDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILI
                     AIYDEEAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGI
                     RSIQVSNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKM
                     AIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLKKRWSSIPNFSYKK"
     gene            20280..20681
                     /locus_tag="IWM52_001003"
     CDS             20280..20681
                     /locus_tag="IWM52_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9973272.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            20675..20920
                     /locus_tag="IWM52_001004"
     CDS             20675..20920
                     /locus_tag="IWM52_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9973273.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(21244..21465)
                     /locus_tag="IWM52_001005"
     CDS             complement(21244..21465)
                     /locus_tag="IWM52_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973274.1"
                     /translation="MRNEIANITIIRETLQNHTANEISKHTGLNLSTIKKLKSGERSI
                     EKLNLHDAICLTEFGLKNNRKNVEINIWK"
     gene            complement(21627..21699)
                     /locus_tag="IWM52_001006"
     tRNA            complement(21627..21699)
                     /locus_tag="IWM52_001006"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21664..21666),aa:Lys,seq:ctt)
     gene            21775..22005
                     /locus_tag="IWM52_001007"
     CDS             21775..22005
                     /locus_tag="IWM52_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9973275.1"
                     /translation="MDFQDYEKIFESYPSILKITDLTKMLNIGKNKAYNLLKDRSIEN
                     IKIGARYKIPKKAVIEYILKIQDEGKNHAIIK"
     gene            22049..23218
                     /locus_tag="IWM52_001008"
     CDS             22049..23218
                     /locus_tag="IWM52_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9973276.1"
                     /translation="MKLRANIIEKRGTLQVVVSYKDSNGKIRQKWKDTKLKKKGNKKR
                     AEKIRDEFLLELESQLAMECECANPNLLFYDYLLNYLEVSKKQVAFNTYVGYKHYVHN
                     RIYKFFYPKKIKLNRLKPYHLQEFYQYMLNDNCTANTVIHYHAFIRKALQEAVITELI
                     STNVADKVRKPKKKQFVSQVYNQQEILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGL
                     KWSAIDFLNKKIMINHVIIENPENMSQLIKKDQTKNSSSYRTLPLVETIERALVNQAK
                     WQQDNRILLGDDYILEDNEYVFTMEDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRH
                     SNASIMLDSGQNMKSIQEWLGHASYSTTANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            23792..24361
                     /locus_tag="IWM52_001009"
     CDS             23792..24361
                     /locus_tag="IWM52_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAP9973277.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            24348..25208
                     /locus_tag="IWM52_001010"
     CDS             24348..25208
                     /locus_tag="IWM52_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9973278.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            25398..26102
                     /locus_tag="IWM52_001011"
     CDS             25398..26102
                     /locus_tag="IWM52_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9973279.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            26107..27942
                     /gene="walK"
                     /locus_tag="IWM52_001012"
     CDS             26107..27942
                     /gene="walK"
                     /locus_tag="IWM52_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAP9973280.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            27939..29255
                     /locus_tag="IWM52_001013"
     CDS             27939..29255
                     /locus_tag="IWM52_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973281.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            29256..30125
                     /locus_tag="IWM52_001014"
     CDS             29256..30125
                     /locus_tag="IWM52_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973282.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            30180..30989
                     /locus_tag="IWM52_001015"
     CDS             30180..30989
                     /locus_tag="IWM52_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9973283.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(31092..31721)
                     /locus_tag="IWM52_001016"
     CDS             complement(31092..31721)
                     /locus_tag="IWM52_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HAP9973284.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            31891..33183
                     /locus_tag="IWM52_001017"
     CDS             31891..33183
                     /locus_tag="IWM52_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9973285.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(33259..33921)
                     /locus_tag="IWM52_001018"
     CDS             complement(33259..33921)
                     /locus_tag="IWM52_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP9973286.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            34146..34430
                     /locus_tag="IWM52_001019"
     CDS             34146..34430
                     /locus_tag="IWM52_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAP9973287.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            34481..36106
                     /gene="groL"
                     /locus_tag="IWM52_001020"
     CDS             34481..36106
                     /gene="groL"
                     /locus_tag="IWM52_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAP9973288.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT        12726 a         5311 c         7256 g        11115 t
ORIGIN      
        1 atttatcaat gaaaattgat tttcttaagg ttttacaaaa aaaaaatctt tttttttcgc
       61 aaacgaagca ttcaggagaa attcaattta ggatgttttc ggatatttct actatcgtaa
      121 atactatctt tattttttct gttgaattac ctgtgatagg cgttttagtt cttgtagcgg
      181 tatttattat aagtaaagtg aatcttattt ttctgatttt ttcactaata atgatgggag
      241 ctatggtgtt acagtataat ttatttcaat taccgcttaa gaaaacaatc gagaatacaa
      301 aaagtgttaa tcaaagtatt acaggagaat tgaatgcaca ttttgatcaa ttagaaatta
      361 taagcgcata taattttgag aatatctcta ttttaaaatt cgattctaga tttaaagaat
      421 tatttaatgt tttcaaaaaa caaaataaat tactaacact gggaagctgt ttggcacagg
      481 ttattttcca attatggatt ggaggaatat ttcttattga tgttatttta ttagctaaag
      541 gaacaataag tattactaat tttgtaatag tatttatgta cgctaacatg gtccctactt
      601 tatggactac tttaattagt attatttgga aatggcaaga ctattcagtg tcattaaata
      661 gatattacga atattattca aataacaaca aagtggctgg cacaaaaaaa ataaataaaa
      721 taaattctat tactggtcaa tcattaaatt tttcatataa caaaagaaag gtattaagca
      781 atataagttt ttcaactgaa atgaataaac tattcatgat atcaggagaa aatggaacag
      841 ggaaaacaac actgatgaaa ttatttacta aacagttgcc ggttagtaat aatcaattat
      901 tcgtcaacgg gatagatatt aatatgattg ataacgaatc attaagaagg ttaatttcaa
      961 tcgcaacaca aataccatat ataattaata gctccgtgaa ggagaatatt ttaatgggga
     1021 ctaaaaacta tgataaaaaa aaattcagta aattaataga actattggga ataagtaaat
     1081 ttatgacgaa attaggtatt actttggacg acaatattta tgatgaaaaa atattattat
     1141 ctggaggaga aaaacaaaaa ataagtttag ctagggcgct tattaaatca gcttcagtta
     1201 ttatattaga tgaaccattt agaaatttag ataatgaaac tcgcgataat ttgagggatt
     1261 atatactaaa tacaaaaaaa gaagtttgtt atataattat aactcatgat cacattctag
     1321 ataatttttt ggattcaaat gatgaaatat tatatttaaa taaggagtat gataataatg
     1381 aatagcaaaa aacttcagat gggtgaaatt gttagaggtc ctagtcaaat atctattgat
     1441 gttacatata aatgtaattt tagatgtaaa cattgttaca atgcgagtgg tggagacaat
     1501 tgttttatgg ataatgagtt gactgatgat gaactgttag aacttacaaa agatatatgt
     1561 aatttaaagc catataatat ttgtttctgt ggaggagaaa ctttactgag aaaaaaaata
     1621 gttttatctt gtgccacttt attaaaagag gcaggtattc ctaatattgc tattgttact
     1681 aatggatcat tagtaactca ggatgttgca aaagaattat tcaaagcagg tattaatcga
     1741 tttcaagtaa gtttagatgg aaaaaatgct agtagccatg aagcacttag attatatcca
     1801 aacagttttg aaaaggcaat taatgctatc aaattattta gagaattgtc cgaagaagtg
     1861 gatgttgctt tttgtcctac tagttttaat tacaatcaat tacgagaagt atatcaacta
     1921 gtaaatactc ttaaagtgaa gtctttaaga attcaaccat ttatgcttag tggtagggga
     1981 atgcaaaata gggaaatagt gccaactgtt tctcaatata gaaaactagt ttctgatatt
     2041 aactatatta aaagaaatga agattatacc aagatagatt ggggagatcc aattgatcat
     2101 atcatccgac acagaacttt attgaaagat ttttcgaatt ttgtttctat taggtcagac
     2161 ggaggattaa tggcatcacc ttacttgcca attcaagttg gaaatattaa atgtcacagt
     2221 ctttctaaat attggcgagc aggactttat aaagtttttt ctttagattt agttcaagat
     2281 atagctactc aagtttcttc aatacaagat atgagtaaag aattaaaaaa aggacatatt
     2341 aaatggctta atgaagatat agaaattgat attctcgatg aatacaaaca tgatatttta
     2401 atttaggttt atcattggaa agtttagata aacaaagtac ttttagaaag gagggaaaaa
     2461 aatgatttct gaacctcaaa tgaataatat gtatgaaagc ccaactataa tgggagctgg
     2521 tgaagctgcg ccaagagctt gttcagcaat tatggtgtgt gtgttattag catctgcagg
     2581 agtaggtatc caagcaggag cggtgtatac ttatgctgta gttgctacag tagctgtagc
     2641 tgcagccgca gtttggaaaa cagtaggatt agttggttag gaattaatag gagactaact
     2701 gtctcctatt gattccaatg attaagctaa ggaggaattt taagaaaata atgaaataca
     2761 ataatttaaa accgaaatta cttaatgaga acatagaata tataagagaa gaaaattatg
     2821 gatatttaac catagttccc aaaaatggaa ctgaattgaa agataacgtt ttcaatccaa
     2881 cagtaagata tatattggct aactgtactg gaaaaaacag tgtaaaaaaa atagcgaatg
     2941 gtttaaaaaa gaagtttcct agtttagaat atgaaaaatt ctatcaagat gttgtaggaa
     3001 ctattaaatt tttcaaacac tataatatta ttgatatagg agattatagt atggaagaaa
     3061 agaaaatata taaaattgaa caaaatttag aattagaatt actaaatgaa tcacattatg
     3121 aagaaattta taattttatc acagatcatc ctgaaagtat gtattttcat gaagatattt
     3181 atcatcaagg aaatagtaaa gaattagaag ttgtccttag atataattta ttttcgttta
     3241 cgagtgaatt ttacttttta agacataaag agagaattgt aggaatggtt tctataattg
     3301 ttaatccatt tcaaaactta tcctacagta tagtaaatta cctcatatgt gagcctgcat
     3361 atactacgac tattttgaca acagtattag aaaatattaa cgaagtttca attttagaag
     3421 taaagaagct agtgattcat ttgctaaatt caacagaaga tgaatcacta atcccctatt
     3481 taaaagattc acattttttt aaagaagcgg tactatctaa agaatataga gagcatgatg
     3541 ttttgagata cgcttacatt tattaaataa aatttctgat agtattttaa taattcggat
     3601 catatcataa gatataggag ggaaaagaag tgattttatt gattttgtct ctcgtattac
     3661 tagttgtatc taatctaatg aaaaataatc ctcctacaaa aattaattct ttttatggct
     3721 ttagaactaa aaagtcaatg aaaaatgaaa aaagttggca tttagcacaa aaattgttgg
     3781 gggatttttc aaaaaaaaaa gttatcccag tatttttgat gggtattgtt tttttcagta
     3841 tagagattta tagtttagct attttaaaaa atgaaaattt tttcgttagt gtattaatgg
     3901 gtgaaagttt aatcctaata ctgattttgt gtttaattgt tcatgaagtg aataaacaat
     3961 tgtgaattaa aaaaataaaa acctagtata atcactttaa taaattgttg gtagaaggtg
     4021 atcaaaatgg gagtaaacga aatgatgaaa gtcagattga gtttttaaat gataccttcc
     4081 tcagaacttc aaaacgaatt caaagattcg ttgacaaatc ttgagcaaga tcatttaaaa
     4141 aaatattttc tcttcatttg acgaaaaatg tttagaaatc ttatataagt gaagataaag
     4201 taattggtgt aattaattgt agagaatagc ctttgagcaa ttgaagcgat acaattattt
     4261 agaaaatgaa cttcctccag catactacat gattccttac gggacgatgt taaagaagaa
     4321 agcaagaaat tttagaagta ttagtagata aactaatggg cttttctcaa tttaagggag
     4381 tagcccaaat gtttatcaaa tatggtgcag atacagtagc aaccatgttt acagaagaat
     4441 atcaagtaga ctattatgaa gtaatagata aatgtgtaca ttttgtttat gaaaaagtgt
     4501 ggaattttat gaattttgcg ataagtgaag aaatcttacg agtacaagaa aacagaacag
     4561 gaatatttac cagagacaga aagcttaatt tgattgcgca ttatatacgg aatcgctggt
     4621 catcaatacc aaattttaat tataaaaaat aattatgaac aatcttttat acaatataca
     4681 caataaatca agtagtattt atgaaaaaag acttcaaaaa cagaatgatt ttctatcagt
     4741 cttagaaatt atagagctaa aaattttggt cacgcgtaga aacgcgtttt ttttgttttt
     4801 ttgatagtaa agaacgtaca aaacaatgag aatttaaaag attgtactac cagttttttt
     4861 accttttcat ttgagtttag agtaagtaaa atattactat aaaaacacat attcttgaag
     4921 aaatcttttt tttgctgtat tcatttagat tatgatagag aaaatacgtg agagggggac
     4981 ctgggatgtg gctatctgat gaaaaaattc agtggcgtag ctatcagaag cgtatattga
     5041 ctaactttgc tacttattta aataacaatt cagtccatct agtggctcca ccaggggctg
     5101 gcaagacctt tttaggaatt gagatgatcc gacgtttgga ttgtaaaacc ttaattttgg
     5161 ttccttccct aatattgaag aaacagtggt taacaacaat cgaggaatat ttcttagtaa
     5221 atccagcaga tagttgctat ctttctgggg cgattgaaca tcctagtcat attacagtgg
     5281 aaacgtatca gaccatttac aaccgactga aggaggagcc aacttatggt gatcagctgg
     5341 agctgttagt tttttgatga agctcatcat ttaaaaaaaa gctggggtga attgttgatt
     5401 gacttgaaga aaaaacaacc gaatctgttg acgatttcct tgacagccac gcctccgatt
     5461 gatagctctc agacggagtg gaagcaatat attgagttaa aatggcctga ttgacgaaga
     5521 aattagcatt cccgagttgg taaaggagga ggttctatgt cctcatcagg attttctata
     5581 tttgattccg gctaatcagg aaactactca gcattatttt acctttttag agaaacaaaa
     5641 tgagctggtg gaagcgttaa tggaaagcca agagacggtg gattatttgc tttctttaga
     5701 atttgttcag catccgcttg agcattcgaa ggaaatttat gaaaattttg agctgttcac
     5761 tagtgcatta atttacttga atcatcataa ctatgaattg atacccgaac attggcagct
     5821 attaggcagt tctaagcagc aaggtaaaaa agcttcagtt ccatctttag gtaaaaaaga
     5881 gctgatcact atttatcagc agatgtttga agatcaaccg gaattgccta ttttttatca
     5941 attgaaaaaa gctggatgat tgtttgatga gtcgctgcat ttgtttccag agtatccaga
     6001 cactgttggt agtcaggcga ttcctgagaa aaagcaagca gtttgtcaaa ttttgttaaa
     6061 agaagaagtt cagctgaagc aagaactgac agcggtcatt ttgatggatc gtatttgcaa
     6121 ggaggtatta gcaggcagtg atggtacctt agcttatggc gtggttcctt tgtttgatga
     6181 agttgttcgt ctattaggta acactcagca aatcggtgcc atctgtggag agttcttact
     6241 gttaccgaaa atgctagtaa ctgttgagct ggcagaacta gcgacgggta tgaatgaaca
     6301 tccttatatg tcgggttatt tttatttgac ggtggatgac aaaaaccgcc aagcattgat
     6361 gcaagcagtt actcgcctat taaccgaaaa gaagttgaat attttggtag gaaccgttag
     6421 tttgctagga gaaggctggg attgtcctgc aattaatctg ttgatcttgg ctaatcaagc
     6481 ttccactttt gtgcaaacac aacaactacg agggcggggc ttacgcctag gtgcacaaaa
     6541 taaagtaacg aacatttggc atttagcgat tggattaccg ggagttcctc tgtctgaaca
     6601 gcctgatttg aaaagaatgc tacggcgttt gggcaatatt tcaggtctga cctttgaatc
     6661 ttcaccaaaa attgagagtg gtggcgatcg ctttgcttta ccactggaat atacgaccac
     6721 cacgatggaa acttatactc agtcgatgtt ctatttttct tgtgaacgaa gtcggttgaa
     6781 agaacaatgg agtactgctt tagctgatgg ctggcaccta tctatgccaa tcgtcgttag
     6841 gaaagtcgat caggtaaagc aaaaacaaga aacttcattg ccgatagtag taaaaaatat
     6901 agagcctcta agcttctggt cagctatatt taaaggtaac ttgctggaat atttccaaat
     6961 tcgtaggcga caaaagctat gggaaaagga atgtgcccgc aaaaaaagct tggccatatg
     7021 cgttcaaacg acgttgcagc gccttggtga agttagtgat agaaaagtaa tcgtagcggc
     7081 tgatcagcag gaatttagat gctatcttga gaatgctggc tatcgagaag gacagctttt
     7141 tactgaagct atgagggagc ttttagcacc gctggagaat ccgcggtatt taatcgaagg
     7201 gaaaaagata gttctgacgg taccagaacg ctttggccga aataagcaaa tcgctaacct
     7261 ttttctaaac gaattaaatc aatcgtcgct gcccaaatgt cagttggtgt atactagaaa
     7321 tcctgaagga cgccaaaagc tagtgactta tcgaatgagg caagtcttcc agacccgcga
     7381 tgaagtaatt attcaaaaaa acatttggac gtaagttgat gaaaataaat tttgctagag
     7441 tagaacaagt ccgagaatta agttgctact taactgataa tgccaaggac aggagaatct
     7501 tttcttgacg actgaaagaa actatttgac ttatataacg gtgtgtgatg aatatttgca
     7561 aggcattctc ttaaaaagta ctcgtaatag tttttttgaa aacaaaacag tactttatta
     7621 ataggactga cagagcgata taagaacatc tgcagacaga ggtgaatgct tttctcatga
     7681 tcgatgaaac aatggtactt cggatattct tgcttttagt ttgaaccgtt gtgattttct
     7741 ttgattccta aaagtagttt tatagaatga aaatcaagca tacggaatgc tcctttgatt
     7801 gtgttgtggt gattcaatct aatggagctt ctatatgttt ttttatttgc aaaaagaacc
     7861 gatagaatat ttttatcagt cttagaaatt atagagccaa gaaatttcaa gcacacataa
     7921 aaacgcgtgt tttatgcatt ttatgagcaa aaataactaa agaccattac gtaaaacata
     7981 ggtaaatcag cattcaatct caaattatgt gatcaaatgt tgatattatt tgattataag
     8041 ttttcttttg ctattatcag tctgcaaata aacagattat agagtatgaa tgtagatagt
     8101 tcaatctttg tttacgacct cacatgtttt tttactcaag agaaagtaat ataaagtgac
     8161 tatatttgac agaactttac ataaaaaaag tagttagttt tctgtagtaa tcagaaatgt
     8221 aaataaatta aaaaaaagaa atttagcagt tatatatttt taaaatattt tgggtgagta
     8281 gtcaatacaa cattttgttc atcaagcatt aaatgaagat gcaaggatgt taagaagtta
     8341 gcttcttcag ggtcttgcat ggtcaaggct ttaaatagtt gatgatgttg ttccataatt
     8401 ttttcccatt ttaatccttc cgtttttact cgtaaccaac gaaatcgttc caagtgcgta
     8461 ttattatctt ccaaccattg ccatacttct tcccgttctg caatttcaaa acaagcttta
     8521 tgaaataaat tatcattatg aaaaaaacta cgagcattct tttgactaac agctttttct
     8581 tgttctgcaa ttatagtttc taaaacttgt ttcttttgat tattcatctt ggcacaacac
     8641 tctagcataa tttgtcgttc taaatgttct ctgacaaaac gagcattttt tgctaaatct
     8701 aagtcgatta atgagatata atttccactt tgaggaattg tatcaacaag tccttgatga
     8761 cggagttgaa tcaatgcttc acgtattgga gtacgaccga tagaaagcgt ttcttccaat
     8821 tttttttctg atactttttt tccaggttct aagtcagcgt agataatttg ctgacgaata
     8881 gattgataag cttgattttg gagactagtt ttaattgtca tatttaatcc ctctcaaaat
     8941 ttcatttttg aatttgatat tttagttcta tgctcgttat ataatatcaa atttcaaagt
     9001 aacatactag ctattaaaaa aacaaaaaat actcaaataa gatcaaagag aaattaagct
     9061 ttaatttaag ttagttctaa aaaatatata ttggtgtgct tttactaaaa aaagacacaa
     9121 taattctgac atggtagaaa aaagaatatt tttagttttt tcttattgac agcgattaca
     9181 ttcgatttta tactacaatt gtaacggaaa ctaacatatc agtatggagg ggtaaatatg
     9241 gttttgatta aagactggca agattctatc gaaggattga acaaaatgca agttcaacta
     9301 ccgtatttta atgtcgaagc ggtcaaacaa gaaggtcaga aacatccagt gtggcttcat
     9361 tttggtggag gaaatcttta tcgaggattt catgcagaaa ttgctcaatg cttagcagat
     9421 caaaatgaat tagaatccgg agtagttgtg tgtgagacat ttgatgaaga agtagtagaa
     9481 aaagcttatc atgactacaa caatgatatt ttggagattg ttatgggtga agatggtgaa
     9541 ctcaaaaaaa gacttttagc agtaacagct gatagtttat attgcaatcc aaagaatata
     9601 acggactatc aaagagtggt taaatatttt gaagatccta cattacagtt tacaacgttt
     9661 actattactg aaaaaggata cagtttaaaa gattctaaag gacaattttt ttcggtagtg
     9721 gaagcagata taaaatctgg acctaccaat cctcaacata cgatgagtat tgtgacatcc
     9781 ttgttgtata gcaggtttaa tgctggagag ttaccgattg cgatggtttc tactgataat
     9841 ttctcacaga atggtcaacg ttttcaagat agcattttta tgattgcaga agcttggcaa
     9901 aaaaaaggat acgtttctga aaaatttatt gcttatttaa aggataaaga aaaagtgact
     9961 tttccttgga gtatgattga tagaattacg ccaaatcctt cggaaagtgt caaaatggat
    10021 ttattgaagg cgggtttcga agacttagat ataattcata ctgcgaaaaa taccaatatc
    10081 gctgctttta ccaatactga aaaagtccat tatctagtca ttgaagatag ttttccaaat
    10141 ggtcgaccta aactggaagc ggctggtgtg attatgacag atagagaaac agttgataaa
    10201 gcagatatca tgaaggttac gacatgttta aatccgctcc atacggcatt agcaataaca
    10261 ggttgtttgt tggggtataa atcaattcag gcagaaatgc aagatgatga tttggttggc
    10321 ttaattaaag ggataggtta tgtagaaggc ttaccagtag ttgaaagtcc aggcgttatt
    10381 gatccaaaac aatttattga tgaagtaatc aataaacgtt tgcctaatcc ctatattcca
    10441 gatacaccgc aacgaattgc aacagatact tctcaaaaag tagcgattcg ctatggtgaa
    10501 acaattaaaa agtatgtcga aaaagacgga aatgcagaaa atctaacatt tattcctatc
    10561 gctattgctg cttggttacg ttatttattg gcagtcgatg atcaaggaaa aaattttgtg
    10621 ccaagtcctg atcctttatt ggtagagttg caggaaaaat tctcagtatt gaaattaggt
    10681 gaacaagatc ctgcaaagat tcattgtgcg gtacaggaaa tcttagaaaa taaggaaata
    10741 tttggtttag atttatatca agttgagata ggaaagaaaa ttgaaaaaat cttttgcgaa
    10801 atgctagtag gtcctcaagc agttcgtaaa acaatcaata aatatataac agaagttgga
    10861 ggaaagtaac tatggaaatg acttttagat ggtatggggc aaatgaagaa aaaatctcac
    10921 tacaagaaat tcgtcagatc ccagcaatga aaggaattgt tggaacatta atggatatac
    10981 cagccggaga ggtctggccg agagaacgaa ttaaagcttt aaaagatgaa gtcgaagcca
    11041 atggccttac cttgaaagtt attgagagtg ttaatgttca tgatgatatc aaaattggtt
    11101 tgccaacacg agatcaatac atcgagaatt ataaacagac gattcgcaat ttggcagaat
    11161 ttgggattga ggttatctgt tataatttta tgccagtctt tgattgggta aaatcagatt
    11221 tggattatca tttagatgat gggtcttcta cgttagccta tatttcaaaa aatattccta
    11281 ctgatccaga ggaaattatt agaaaagtag aaaattctac taataatttt gaattgccgg
    11341 gttgggaacc tcatcggtta tcacaaatca agcaattatt agaagcctat aaagaagttg
    11401 atgaagcgaa attgcgagag aactttgcat attttctcaa agcaattatc ccgacatgtg
    11461 aagaagttgg gattaaaatg gcagttcatc cagatgatcc accatattct attttcggat
    11521 tacctcgtat tgttaaaaat cgtgacgatt tagaatggtt gtgtaatgta gtggattctc
    11581 cttctaatgg tattacgtta tgtactggaa gtattgcaga acgaccagat aatgatgtct
    11641 atgaaattct atctgaattt tgtcaacggg atcggattcc ttttgcacat gttcgtaata
    11701 tcaaatattt aagtgataat ggactcgatt tttatgaagc gccgcataaa tcagaatatg
    11761 gttctctaga tatgtataag atattaaaaa caatgtacga taatggattt aatggctata
    11821 ttcgtccaga tcatggtcgg atgatttggg gagaaaccgg tagacctggt tatgggttat
    11881 atgaccgagc tctaggtgca gtttatttaa atggtatttg ggaagcgctt gaaaaggggg
    11941 cgagtaaata attatataat ggagtctatt tagaagaaaa attttctctt ctaaaaacta
    12001 agggaaggaa aggagtgctt aaatatgttt ttagataaga actttttatt agagaatgaa
    12061 atagctaaaa gactttttca taaatttgca gaacaccaac caatcatcga ttatcattgt
    12121 catttagacc cagcatgttt gctagaaaat aagcaataca agaatttgac tcaggtatgg
    12181 ttaaatgata aaggagctgg agatcattat aaatggcgat tgatgcgagc taatgggata
    12241 gaagaagagt tgatcagtgg tgcaggtaat gattatgaaa aatttttagc ttttgtaaaa
    12301 acagtggaga aggcgccagg taatcctatt tttgaatggt ctcatttgga attacaacgt
    12361 tactttgata ttgatttgac aatttctgag aagaatgcac cagaaatttg gcgacgtgcc
    12421 aacgaaaaaa tccaaacgga ttcctatcgc ccaaaagaat tgctgaagat gatggatgtg
    12481 aaagccattt gtacaacgga tgatcctaca gatgacttgc gatatcatac tgagctttct
    12541 aaacaggata attctttcaa ggtattacct actttccgtc cagataaggt ctggagtatc
    12601 aaatcatcag gattttcgaa ctatatttta aaacttagtc aagcagcggg tataccaatc
    12661 aaaaacttta atgggttgaa gcaggcattg agccaacgag tggattattt tcatcaaatt
    12721 ggctgtcgat tagcagatca cggtttgaat acttattttt atgaaatagc agatgaaaaa
    12781 atactcgaca atctgttaat aaaagcttta gcaggaaaaa cggatttcca tttatcagaa
    12841 attgcacaat ttgttacagg tattcaatta catttgatgt ctgagtatac aagatatggt
    12901 tggacgatgc aaatgcattt aaatgcttta agaaataatt caagtatatt aaaggaacaa
    12961 attggagtag atgtcggtgg tgattcgatg ggggatcaag ctagtttgac acaaaatgtt
    13021 gtagcattgt tcgacactgc tgaaaagagt gggtgcttac caaaaacaat tttatattca
    13081 ttaaatccaa ccgattggct tccactagca acagcaatgc aaagcttcca agggggtatg
    13141 attcaaaaaa tccaattggg atgtgcttgg tggttcaatg atacagctga aggaatgcgt
    13201 caacagttga ctacgatggc gcaacaaagt ttgttgtcga attttacagg tatgttgact
    13261 gattctcgaa gttttctaag ctatcctcgc catgagtatt tccgtcgagt attatgtcaa
    13321 ttactaagcg agtgggttaa agcaggtcga attccagatg atgaaatgta tcttagtcag
    13381 atagtggaga atatttgtta tcgtaatgcg tataattatt ttaatttttt tgaaatcgaa
    13441 agtagaaaat aatacaaaaa ataaaagctt aaatttattt attgtaagtt agtacaacta
    13501 gctagagtat gtatccataa aagaaggaga tagatatgaa aaaagtagaa attttaagta
    13561 agttagaagc ggctggagtc attgcggttg tacgtggaaa aaataaagat gaggccatta
    13621 aagcaataaa tgcaattact gcgggtggaa ttaaagggat tgaactaact tttacagttc
    13681 cgaatgctga agaaataatc aaagaaattg ctaaaaaata tgctgatcaa aaagatgtaa
    13741 taattggagc cggtacagtt ttagatgcgt ttactgctcg tattgcaatt atggctggag
    13801 cagaatatat cgtaagtcca tcttttgatc gacaaacagc agagatttgt aatttatatc
    13861 aaattcctta tttaccaggt tgtatgacaa ttactgagat aaaggaggca cttaccgctg
    13921 gagtagaaat cattaaacta tttccaggtt ctgcttttgg tccatctgtt gtaaaaacgt
    13981 atcaagctcc attaccatat gtaaatataa tgcctactgg cggagtaagt ctagataact
    14041 tagaagagtg gtttgaagca ggcgttgttg cagttggtgt aggtgggaat ttgttggctc
    14101 ctgttgaaaa aggtgatttt gaagaagtaa ctcgaatagc tagtaaatat atagataagt
    14161 tttatgagat aaagaattat taggagaaaa aataaataat actataaaat gtaaaaaata
    14221 aatactaact ctttatactt cagagtatag tacgtaagtt ggcagttcaa cagaatgaaa
    14281 acgcttaaca ataggaagga gcgcaaatta tatggaaaga aaatttggtc taagagacaa
    14341 aataggatat atgtttggag attttgggaa tgatttcact ttcatttttg ctagttcttt
    14401 tttaatgatt ttttatacaa aagtattggg gataagcgga gctaccgtag gaacattatt
    14461 tttagtagca agatttattg atgcagttac agacataaca atggggcgta ttgtggatgg
    14521 atcaaaacca tctaaaactg gcaaatttaa accgtggatt ttacggatga gtggtccagt
    14581 cgctttagca agttttttaa tgtatcaaac atcaatggca tcagcttcta tgagtattaa
    14641 agttatatat atgtatgtaa catatatact atggggaagt attttttata catctattaa
    14701 tattccttac ggttcaatgg catcagctat cacttctgtt gcagaagaaa ggacagcgtt
    14761 atcaacattt cgtagtatag gagctacctt agcaggtatg ttcattggcg tgttaacacc
    14821 aatttttatt tatacaagag atagttctgg agctcaaatt gtacgtggtg gatcaacttt
    14881 tacaattgtg gcaggagtat tttcagtgtg tgcagttatt tgttacgtaa tatgttataa
    14941 attaactgta gaacgggtta aaagtgatga atcgcagtta aaagtttcat tgttgaaatc
    15001 tttaaaagga ttagctaaaa atagagcgtt gctttctata atagctgcag ctattttttt
    15061 gttgcttgct cagcttttaa caatgagtat gaacaattat gtatttccag attattataa
    15121 taatgcacag ggaatagttt taatgaactt tatcaatcct attttgatgt tggtacttgt
    15181 ttcacctgta agtttaattt tcagtaaaaa atacggtaaa aaagaattgg cgtcagtagg
    15241 gatgttcttc tctgctattg tatatttact tttatttttg ataaagccta gtaatatgta
    15301 tgtattctta gttcttactt ctgttggata tatggggtta ggtattttta atacagtcat
    15361 ttgggcgaat attactgatg taatcgatga tcaagaagtg aaatctggcc aaagagaaga
    15421 tggaactatt tatgcagtat attcttttgc ccgaaaaatt ggacaagctt tagctggcgg
    15481 tgtcggaggt tggactcttt ctataatagg gtatgattcg ttagcaaaag tacaaagtgg
    15541 agaagtttta caaaagctgt ataactcttc aacgttgatt ccagcagttt gtttctttgt
    15601 tgtaggattg attttatttt tcgtatatcc attatctaaa gaaaaagtat tagaaaatta
    15661 taatttgcta aatcaaaaac ataactagga gaaaaaacat gaacagttgg atattttgta
    15721 ttgattcaga tggttgtgtc atggatacaa tgacttataa acacaatctc tttttcgggc
    15781 caattgctgc agagattttt gatgttcatc atagggaaaa attcctacag gattggaata
    15841 atattaattt atattcagag actcgtggaa taaacagatt tttaggttta gttaaaacat
    15901 tagaaaaaaa tgatgtacca tcgattaatc gattaaagca ctgggtatct gtaacaaaag
    15961 aattgtctaa tccatctttg aagagggaaa tagaatactc tcagtcagag gatttattaa
    16021 aagctttaaa atggtcggaa gaagtcaatc gacagattag tcaagctaaa ggattagata
    16081 aacccttttt aagtgctaga gatactataa aagctctgaa aaaatatggg aagataatta
    16141 ttgtaagctc agctaataag gaagctattc aagaagaatg ggaaagacat gaattattat
    16201 ccttagttga tgaactgtgt tgtcaagata aaggaaaaaa agaagacatt attcgaagtg
    16261 taattgaaaa tggttgtgat ttggataaga tattaatgat tggtgattct cctggtgatc
    16321 tagaagctgc aaataagaat aaagttttct tttatcctat tttggttaat aaagaaaaag
    16381 aatcatggga aaacttaaga actgatattt tatttaaatg tttatcggga gagtatatag
    16441 atattgaaca aaaatatata gatacttttt ggaaaaatct tactaataag tagaggagaa
    16501 agaaaatgac tataaaagta gacttacgaa aaaaaccata tcatttagat gaagatgcta
    16561 tttcttggat tgaagagacg attgatgcta tgactttaga agaaaaaata ggtcaattat
    16621 ttgttaatat gggatctagt cgtgataaaa attacttaag tagtatggtc aatcagtacc
    16681 atattggagc tgtacgatat aatccaggaa ctgctgatga ggtatatgat caaaactata
    16741 tcctacaaac agaaagcaaa attcctttat taattgctgc taatacagaa gcaggtggta
    16801 acggagcttg tacagatggt accgaagttg gtgtagaagt aaaaattggt gcaacaaatg
    16861 atgcgaaata tgcttatgaa ttagggagag tttctggaat tgaagcatcg gctattggat
    16921 gtaactggag ttttgctcct attgtagata ttaatagaaa ttggagaaat cctatcattt
    16981 ctagtcgatc ttttggacaa agccctgata aagttttaga acttagttta gcttatatga
    17041 aaggtattca agagagtgga attttgcctg cagctaaaca tttccctggt gacggtattg
    17101 acgaacgaga tcaacatcta tcttttagta ttaatagttt aagtacagaa gaatgggata
    17161 attcttttgg aaaagtatac tcaggcttaa tagaagctgg attaccttca attatggcag
    17221 gtcatatcca tatgccagct tattcaaaac attttaatct tgatctgaaa gatgaagatt
    17281 tacttcctgc aactttatca aaagaattaa tcactgactt acttagagga aaactcgggt
    17341 ttaatggatt agtagttact gatgctagtc atatgttagg aatgactggg gcaatgaaac
    17401 ggtcagaatt attgcccact tctattgcgg ctggatgtga tctattccta ttttttaatg
    17461 atccagatga agactttaat tatatgatgg aaggttatta caaaggaatt atttcagaag
    17521 agcgtttacg tgatgcatta agaagaattt taggcttaaa ggctaaacta ggtttgcata
    17581 aaaaagaaaa gaataaaatt ttgttaccga aacaagaagc tatggaaaaa attggtttaa
    17641 ttgaaaataa atcaattttt aaagaggtag ctgatcaagc aattacacta gttaaagata
    17701 aacaagatat ttggcctatt gtaccagaaa aatataaaag gattttactg gtagatgtac
    17761 atggggtaaa aggtgggttt ggtgaagtag ttgctggaaa tagacctcag gccattgatt
    17821 tattgaaaca gattttgtca gcaaaaggat ttgaagttga aaaatacatt tcacctgaag
    17881 aagaattgaa aggattatcg caagaagaaa aaattaaaaa tatagctgga atatatgctg
    17941 ctaagagacc aatcaaagat ttaactgaaa aatatgactt gattattaat atagctaatg
    18001 ttaacccagg tacagttcaa agaattgttt ggccagcaag taaaggtact ccggatattc
    18061 cattttatat tcatgaaatt ccaacaattt ttgtatctgt acagtgtccg tttcatttag
    18121 ctgatgtgcc tcaagtaaaa acttatatca atacttatga tggaaaacaa gaaactttag
    18181 aaatattagt ggacaaacta atgggccttt ctgaatttaa gggaataagt ccaatagatg
    18241 cttattgtgg gttaaaggat acatttattt aatatttaaa ttatatagga gtgatttaca
    18301 tgttatatcc cattatgaca gagacgaggc aagtaattga tttaagcggt ctttggagat
    18361 ttaagttaga tacaaatgat gacttacaag aagaacttgc tttagaacca atggttgaaa
    18421 aagagagtta tccaatttca gttccttctt cttataatga tttatttgaa acagagaaca
    18481 ttagaaatca tgttggatgg gtgtggtatg aaaaagaagt gattcttcct aaaaaattat
    18541 tagaagaaag attagttctt cgttttggat ctgcaacaca tgaagcaaaa gtatttttga
    18601 atggaaaatt gattaatcaa cacaaaggag ggtttacgcc atttgaatca gaaattaatc
    18661 agtttataaa agatggaagt aacagaatta cagtggctgt aaacaatgtt attgacgata
    18721 gcactttacc tgtagggatt ttgaaagaaa cgatacagga agatggagac ataaaaaaag
    18781 aaaatttggt taattttgac tttttcaatt atgcaggcat taatagaccc gttaaattgt
    18841 atacaactcc gaaagactat ataaaggatg tcactattaa tacaaaaata attgattcag
    18901 atgcaaatgt agaatttaat atcgatacag aatggaccgg agaaattcta atagcaatct
    18961 atgacgaaga agctcaactt atagcagaag ctaaggggaa agaaggagtg attcaactta
    19021 agaacccaac actttgggag cctctaaatg cttacttata tagtatgaaa gttagtttaa
    19081 ttaacgaaaa taaagtgatt gatacgtaca cagaagaatt tggcattcgt agtattcagg
    19141 tttcgaatgg gaagtttttg attaataata aaccttttta ctttaaggga tttgggaagc
    19201 atgaggacac gtatattaat ggtcgtggtt tgaatgaagc agtaaatatc aaagatttta
    19261 atttaatgaa atggatcggt gctaattcat ttaggacttc acattatcct tattcagaag
    19321 aaattatgcg cttagcagat cgagaaggta ttgtagttat tgatgaagta cctgcagtag
    19381 gattacattt gaatttcatg gctactttat tagatgacaa cgtagaaaaa catgatactt
    19441 ggaaagaaat acggacagaa aatgctcaca aaatggcgat taaggaattg atagaacgcg
    19501 acaagaacca tccgagtgtc gtaatgtggt ctattgcaaa tgaaccagat tcggattctg
    19561 aaggcgctaa agaatatttt gaacctttag ttgaattgac taaagaactt gaccctcaga
    19621 agagacctgt aacaatcgtt acttacttaa agtcaacacc agatatttgt aaagtaggag
    19681 atattgtcga tgttttatgt ttaaatcgct attatggttg gtatgtttca ggtggagacc
    19741 taaaacatgc aaaatatctg ttgaaaaaag agctagatgg atggatggaa agatgtcccg
    19801 atacgccaat aatgtttaca gaatatggag cagatacagt agctggactt catgatacag
    19861 taccaacaat gtttacagaa gagtatcaag tgggttatta cgaagcaaat cacgaagtaa
    19921 tagataaatg tgacaatttt gttggtgaac aagtgtggaa ttttgcagat tttgaaacaa
    19981 gtcaagggat tttaagagtg caaggaaata agaaaggaat ctttactaga gatagaaagc
    20041 ctaaaatgat tgctcactat ctaaagaaac gatggtcatc aataccaaat ttcagttata
    20101 aaaaataatt taccttttat aaggaatata aaaaaggagt ggctgcttaa ttgccaactc
    20161 ctttttttta taaaaagatt ttctgtttaa agaaggatat ttttttctag ttttttcgaa
    20221 aaaaaggcgc tttctgattg ttgtattagt ggaaagggga aaaaaggagc atatttatca
    20281 tgcaacaaaa ttacaatgag aaatctatac aacatcaatt tgatgcctac tgtaaaaaag
    20341 tattacgaaa cgaaactaag tctattcgaa aacgaaacat taacataaca gataatgagg
    20401 aaccattgac gaattcaaat gaaggtaagt actcccaaac tcattttgat gaacaagatg
    20461 tctatttctt atttggaatg gaaatattaa tttcagatca aaagttatcg gcagccattg
    20521 atcatttatc ggaaataaga agaaaggtta tcctacttta ttattttgca ggttttaatg
    20581 atactgaaat cggtaaaatc ctcaatatga gtacaagtgg tatctggtat caacgaaaga
    20641 aggcagttga acaattaaaa atggggtatg acttatggta gacaacccca aaaaacggca
    20701 gtatccatta gttcctctat ccattatcca atctgctcta gaagaagatc caatttcaat
    20761 caagatgata ctcaaacatt atcatagtta tatagctaag ttatgtttga caaatggatt
    20821 taatgaggcg gggcaattta ttacctatgt agatgagtat atgctaagac agctagaaat
    20881 taaattgata gaagcgatct taaaatttaa aattaactaa ctaaaaagtc ccctttccac
    20941 ttattctgtt taaaagaatc ttctaatagt taaaggatta gtagattctt ttaaacagaa
    21001 aacctattga tcattgaaat ttaaatacat tctatcagta cgttaacact tcaaggagca
    21061 tattttagtt gcgccatgat ttcttactgt aaagaaggag cgataaacat tctaaaataa
    21121 tttttatagt caataaaaag cgatgatttg aagttgggat aatgatactt ccgtaaggct
    21181 cgccgtaaac ttggagagag gtgaaattcc tatgtgcttt tgacaaaagc agctgattaa
    21241 gaattatttc catatgttga tttcaacatt ttttctatta tttttaagac caaattcagt
    21301 gaggcaaata gcatcatgta aatttagttt ttctattgaa cgttctccac tttttagttt
    21361 ttttatagta ctcaaattta atcctgtatg tttagagatc tcatttgcag tatggttttg
    21421 taaagtttct cggattattg ttatattagc aatttcatta cgcataatta tgttcctttc
    21481 ttatcaaaaa tatcaatgac tacagtgggc ttttagtgaa aaaaaggggt actacagtct
    21541 cttttgaaaa caaaaaaata cagtatatac gttttctaaa tgctaagcac caacgtttac
    21601 actgtattta agggtttggc acccaatggg ctgtgagggg ctcgaacccg cgacccgatg
    21661 attaagagtc atctgctcta ccaactgagc taacagccca aattaatact ttgttattct
    21721 aacacctaaa ataaaaaagt gcaatactta ttatcagaat tgagagtgaa ttttatggat
    21781 tttcaagatt atgaaaaaat atttgaaagt tatccatcga tattgaaaat tactgattta
    21841 actaaaatgt taaatattgg aaaaaataaa gcttataatt tgctgaaaga tagatcaata
    21901 gaaaatataa aaattggagc aaggtacaaa atacctaaaa aagctgtcat tgagtatata
    21961 ttgaaaatac aagatgaagg taaaaatcat gctataataa aatagtcaaa atttttgccg
    22021 atatttcagt ttaagaggag agattgaatt gaaattaaga gcaaacataa ttgaaaaaag
    22081 aggaacatta caagtagttg tatcttataa agactctaac ggaaaaatta gacaaaagtg
    22141 gaaagataca aaattaaaaa agaaaggaaa taaaaaacga gcagaaaaaa ttagagatga
    22201 atttttacta gaattggaaa gtcaattagc tatggaatgt gaatgtgcta atccaaattt
    22261 attgttttat gactatttgc ttaattattt ggaagtatca aaaaaacaag tagcatttaa
    22321 cacatatgtt gggtataagc attacgtcca taatcgaatt tacaagtttt tttatcctaa
    22381 aaaaattaaa ctaaataggt taaagcctta tcatttacaa gagttttatc aatatatgtt
    22441 aaatgataac tgtacagcaa atacggttat tcattatcac gcatttatac gcaaagctct
    22501 tcaagaggct gttattacag aattgataag tacaaatgta gcagataaag taagaaagcc
    22561 aaaaaagaaa caatttgttt ctcaagttta taatcaacaa gagatcttga aattacttga
    22621 cattttgtca gaagaaaaac tctatttggt agttctttta acagcatttt atggattgcg
    22681 aagaagtgaa gttttaggat taaaatggtc tgctattgat tttttaaaca aaaaaatcat
    22741 gataaaccat gtaatcatag aaaatccaga gaatatgtca cagttaataa agaaagatca
    22801 aacaaagaac tcttccagct atcgtacatt acctttggtt gaaacgattg aacgagcatt
    22861 agtaaatcaa gctaaatggc aacaagataa tcgtattctt ttaggagatg attatatttt
    22921 agaagataat gagtacgtat ttactatgga agatggacgg ttgatgaagc ctcaatatgt
    22981 aacgcatcgt ttgagtaagt tgattaagaa aaatggattg aaaaaaattc gctttcatga
    23041 cttgcgtcat tctaatgctt caataatgtt agatagtggc cagaatatga aatccattca
    23101 agaatggctg ggacatgcat cgtattcgac aactgctaat ttatacacgc atttaaccgc
    23161 aggagtaaaa gatcgtgcag cagaagcatt agaaaacgca tttgggttta aagaataaaa
    23221 aaacaaaaaa caatatcttt ggggtatcgc ttcttgtttt tagaattttt ttgttggcaa
    23281 tttgttggca gtatgttggc aatgaaaaaa ttcacaaact aaaaacctat ttataaagct
    23341 ttataaaact tttttcacaa atagagcatt tggttaagag tcagctgctc taccaactga
    23401 gctaacggcc cgtacaaaaa gtacttaata atagtagcac taagaatttt aaaatgcaag
    23461 actacattta aagaaaatca gaaaaatctt tacttcctta taggaaatca ttcatagatt
    23521 attcacattt gctcgctatt atataagcat accaacaaac aaagttcttg caagagaaca
    23581 aaccctaaca cttttttcat tttttactcc tttcccgccg tctccccggc gggtctcctg
    23641 ttcttaacga taaaagacaa gaaggcttag ccttcttgtt ttttgttttc atggaatccc
    23701 gctaatcaaa tcacctagtt aaagcaacta gattgaatgt ttatcataag tctgatatca
    23761 tgtttatgaa atgaaaaaag gagcggttat gatggaaaaa atgcgtcagc aattaatgga
    23821 gtgggggaag gaactagaaa ctttccgtct gcctcactgg gaagaactcc ctgacttaga
    23881 actttatatg gaccaagtca gaacgttagt tgatcgttat ctttcacctg tgatacaagg
    23941 agaaaagcat ccattgctta cctcttctat ggtaaacaat tacgttaaac taggtttgat
    24001 accagcacca gttaaaaaaa gatataacaa agaacacgta gcatttttac ttgcaatcac
    24061 tacgttgaaa caagtattga ctattcctga aatcaaagaa gggatattgt tccaaggaaa
    24121 aacagtagga attcgtgaag cctataatct attttgcgat gaacaagaag cagctgtgtg
    24181 gatggtcagc caattagcac aaggtaaatc tcatccgcaa aaattcgacc aggcaacccc
    24241 tgtagaatat attgctgtaa gagcggcaac tttatcgttt gcaatgaaac ttcttgcgga
    24301 aaaaacgatt gttctagaaa ccgaatattt aaaagaggag aaaacaaatg aaaaacaata
    24361 aaatcgcaat tttagtagat tcaggtacgg atgtgccaga agaattgata aaaaaatacg
    24421 agatgtatat gatcccattg aaaattattt ataaagatca ggtatataca gataaaattg
    24481 acattacctc tgaagagatc tatcaacgtt tgccagagga aatccctggt acatctttgc
    24541 cggatggtga gtcgatcaca gaaatctttg agcaaatcaa aaaagacggt tatgaaaagg
    24601 tacttgcggt aacaatttcc agtggattga gcgggacata taatgtggtt cgtttaattg
    24661 cacaacagca agaagaattg gaagcatatg tactagatac gaaaaatatt ggtattggtg
    24721 caggtttttc tgcgatccaa gcagcaaaat ggctggaaga aggagtagaa tgggatcaac
    24781 tgatcagtaa tctgaatgag ttagtcgaaa gaacaaaagt tttctttaat gttgcgacat
    24841 tggaatatct tcaaaaaggt ggacggatcg gtttagttgc ttctattgta ggaaccgctt
    24901 taaagcttaa cccaatcatc tcatgtaatg aagaagggat ttattacaca gtaggaaaag
    24961 ctaggggacg taaaaaaagt ttggataaaa cggttgctta cgtcaaagaa aaaatcggta
    25021 atgcaaaggt cttcaactta gctgttgccc atggggatgc gaaagaagaa gcaattgaaa
    25081 tggaagcgag acttaaaaga gagttcccac aagcaaacca aatctacttt ggccagattt
    25141 ctccggcatt agtagtccac actggtccgg gattattggg tgtcggtgta caagttttag
    25201 aaggataaat caaaaaaagg aaacactgag gtgaaaatca gtgttttcct tttttattga
    25261 agttagaaca aaaaataata aagtattttg cactttcatt ttaattttct cttttgtttt
    25321 ataggcaaag atgaaacaaa agcagaaata cggtataatg agaaggtatg acaataatat
    25381 ataagaggtg gctctttatg aaaaaagttt tagttgtcga tgatgagaag ccgatttcgg
    25441 atatcgtcaa attcaattta gccaaagaag gctatgatgt ttatactgca tatgatgggg
    25501 aagaagcctt ggaaaaagtt gctgaggtag aacctgattt gattttactt gatttgatgc
    25561 tacctaaaat ggacggctta gaagttgctc gtgaggtacg caaaacttat gatatgccga
    25621 tcattatggt aacagccaaa gattctgaaa ttgataaagt cctaggctta gagttaggcg
    25681 ccgatgatta cgtgaccaag ccattttcca accgagaatt agttgcacgt gtcaaagcaa
    25741 atcttcgtcg cggtgccacg gctgcgaagg aaccagaaga ggcagcacct gcggaattga
    25801 cgattggtga tttgacgatc catccagaag cttacatggt gacgaaacga ggagaaacaa
    25861 tcgagttaac acatcgtgaa ttcgaactac tcttttactt ggcgaagcac cttggacaag
    25921 tgatgacaag agaacattta ttacaaacgg tttggggtta tgattatttt ggagatgtac
    25981 gaacagtgga tgtaactgta cgtcgtttgc gtgaaaagat cgaagataat ccaagccatc
    26041 caaattattt agtgacacgt cgtggtgtcg gttactattt gcgtaaccca gaacaggagt
    26101 aattggatga aaagaaaagt ccgctttttt cgatcagtca attttaagat cgctattacc
    26161 tttattctga ttttattgat ctcgatcgaa attattggtg cctactttat acgtggttta
    26221 gaacgttcca cgatcaatac atttattaaa gatatgaatc aaacggtgga atcattagca
    26281 acaacaatca gcccggaatt gaatcgaaaa gataatgcag atgatgaaga ggtaaatgcg
    26341 aatatcaaaa gatttattga aaacagtgcg actagcgata tcatcgaaat acgggtcgtt
    26401 gacgaaaaag ggatcatccg aggaacaacc gatgtcaatg aacaaagcgc tgtgggaaag
    26461 aaaaatgatt atgttgatat caatgatttc acgactaaac gttatgttgc tttagataac
    26521 gacgataaac gggtgaatat caatgttcaa ccgatcctgt ccccgaccgg cgatgccgtt
    26581 atcggggctc tttacgtcaa aagtaatatc gaacaaaaat attcggaaat caataacaca
    26641 gcagtcattt tctttactgc ctcactaatt gcggcattta tttcaatgat cgtatctgtt
    26701 ttagttgctc gttcgattac acagccgatc ggtgaaatga gagaacaagc cattcgaatc
    26761 gctagaggag actacagtcg gaaagtcaaa gtgcatggac aagatgaact tggacagctg
    26821 gctgatacct tcaatcagtt agctgaacgt attgaagaag ctcaagatac aatggaagct
    26881 gaacgcaatc gtttggatag cgtgctatcc catatgacag acggcgtcat tgcgacagat
    26941 cgcaggggta aagtcattac gatcaatgat atggctgttt ccttgctaga tatcaaaaac
    27001 gaggaagcaa tcggacaatc gattttgaca cttttagata tcgaagaaga atatacgttg
    27061 cggaaattat tagaagatcc aaacgaaatg gtacttgacc gttcaaccag tacattggaa
    27121 agtgatcaga tgatcttgcg agtagacttt tctatgatcc gtcgcgaatc aggttttatc
    27181 agcggattag tagctgtctt gcatgatgtg acagaacaag aaaaaacaga acgggaacgt
    27241 cgtgaattcg tctctaatgt ttcccatgaa cttcgtacac cgttgacaag tatgcgtagt
    27301 tatatcgaag cattgagcga aggtgcgtgg aaagacgagg aagtagcacc aaattttttg
    27361 aaggtaacgc tagatgaaac agaccgaatg attcgaatga tcaatgacct cttaaatcta
    27421 tctcgcatgg atacaggtaa tacccagctt caattagaat atgtcaattt caacgagtta
    27481 gtgaattttg ttcttgaccg gtttgatatg atggtgggaa atcaggaaaa aaattataag
    27541 atccgtcgtg aatttacgca aagagatctt tgggtcgaag tagatacaga taaaatcatc
    27601 caagtagtcg ataatatcat gaataacgcg atcaagtatt caccagatgg gggaacgatt
    27661 acttgtcgct tattagaaac gcataataac gtggttttaa gtatcaccga tcaaggattg
    27721 gggattccta aaaaagatct gaaccgagtg ttcgagcggt tctatcgagt agataaagca
    27781 cgtgcaagag cacaaggtgg gacaggactt ggattagcta tttctagaga agtgattaag
    27841 gcgcatcgag gcgctatttg ggcagagagc aaggaaggaa aagggtcgac tttttatatc
    27901 tcgctgccat atgaacctta tgaggaggaa tggtgggaat gagaatctct gaaaaagtcg
    27961 ttcgtatagg actcattttg atggttgcgt taagcatata cttctcttat gcaatctggc
    28021 tgagcccagc aggaaaaagt tcaatcaacg tagatgagac aaacactcaa atgatcgagt
    28081 ctcaaagcaa tcgaaaaact tccgaagtct ttcttccgct tcatgttaca tggcaccatt
    28141 cgggaatgat ccaagaaacc aacagtgaaa atctcgtgta ccgattgcaa ttgatcatcg
    28201 ataaagcaag atttggaaag ctttctgaag tagtttcagg agacaaatca cagtttgaac
    28261 aggtgaaaaa tctgaactct ggttttgagt tggcctataa tgcgcctttt ttgctaagag
    28321 aatacaagga agcatttgat ttagatttag atgtgtcgaa cttggcaaaa gaaagcgaaa
    28381 atatgtattt tactagaata caattcgatc aaggaaataa aaaaatccga tttttgaatt
    28441 attcgaacca tctcgtatat gaagcaaata tttcgttgaa ttggacagat atcaataaag
    28501 aattaacagc aaaagacaca aaatggacag aaatgacaac agaaccatcg acgatcgaca
    28561 cgcaatatgc tacgaaacat tcgatcaaac tgaaaaagta tagttatatc ctgtcacagc
    28621 aaccctatac actttttaga aatgcttttt tccagaaacc agatagcgtg aaaaataatg
    28681 atgaaagcaa tgaattgatt ttttacggtg ggaatgaaac aatgtctatc cattcggaaa
    28741 cgcaagtagt tgattttcgt ggagagctgc cagagaaaaa agaaaatgct agtgtatttt
    28801 ctgaaagttt cccctatatt agcaaattag gcagttcttt agggaacctc agatattttg
    28861 accgcagtga caatacgatc gattatcgga tattcgtgga aggcttcccg gtttttggga
    28921 cagacagcag aggacagatt ggtgtggaag ttggcggtga aacgaatggt tctgtgcaag
    28981 tgaatatcca aaccagtttg aatacgattc aagtgccgat accttcagac gaagaagtag
    29041 aacttgcaag tacgaatgaa atcatccagc agttaaaagc taagggagca gatagcaaca
    29101 agattggttc agtgatcgtt ggctatacat ggcaaaatgt caaagaaacg aatcgtgtcg
    29161 ttgatctctt acctgaatgg tatgtgaaat ataaagataa ctggtattca gctaacgacc
    29221 ttctggaaaa attacctgga ttggaggcga attaattgga tttcaaaaaa atcgaatgga
    29281 tatttttcgt cgctttttta gggttgaatg tgtttttatt cagcagttat catgacgcgg
    29341 tataccaaga aaacaatgtc attcgctcta atcagactga atcaatccaa caacgtctgg
    29401 ctagcgacga tatcacttac acggggaaga tttcgtctga gaacaagcaa ggctattatc
    29461 ttagcgcgga gcagaccaat cttagcactt cattagcgaa ttatcgcaag gctggtaaaa
    29521 gcggcttgct ggcaagtggt gtgtttttag acggtgatgt tttgacgaaa tctttatcca
    29581 gttcagatgc tgaaaagaac gtgatcgatc cagataaaat tagtagttcc ttggaagatt
    29641 ttttatctaa caaagacaat attttattcg gtaatgatta tgtgtacacg aaaaactttt
    29701 ctcatttaga agaagaacca ctagaaatcc aagcaagcca agagtatgaa ggcatcccat
    29761 tcaatgatga tacagcaaaa atcgttcttt ctttagaaaa aaacgataat gattatagca
    29821 tcacgaaata catgcagacg catttatctg atatcgagca gctgcgcgaa aagacggaac
    29881 tgtatacgga agaggaagca atcaatacat tgtacatcaa taacaaaata ccaagaggat
    29941 caaaaatttt atggcgtcag ctagcttatt cctgtattct gaaagtccgg gaaaaaaatg
    30001 tgtatgttcc tgtttggcat gtagcgatcg aaacaagcga taaagtggtc caaattgaga
    30061 gtgtcaacgc tttcagcaat acgatcgtga caaacaatac tgtaccaaaa gtggaagatc
    30121 aataaaaaag cgagtataat gaaaaaagtt tacaaagtaa gaagggaagt aggagtcaaa
    30181 tggctgaaga aaaaccagct tttaaaatca gtatcctagc aagtggaagt tcgggtaatt
    30241 cactttatat cgaaagtgaa aaaaaacggc ttttggtcga tgcaggactt agcggtaaaa
    30301 aaatcacttc cttgatggct caaattgatc ggacaccgga ccaattagat ggaatacttg
    30361 tgacgcatga acatcgagat catatacatg gcgtgggtgt cttggctcgt aagtatcatt
    30421 tggatgtttt tgccaatcag cagacttggg aagcgatgga accactgatt ggaaatgtac
    30481 cagtggaaca aaaacatcta tttgagatgg ggaaagtcag aacattcggc gatatcgata
    30541 ttgagagttt cggcgtctcc catgacgcag cggctccgca attttatcgt ttccataaag
    30601 aaaatcggtc attcgtcatg ttgacggaca ccggatattg cagtgattat atgcgaggtg
    30661 tcattgaaaa tgccgatggt tatttgatgg aaagtaatca tgatcttgag atgttacgca
    30721 tgggacctta tccgtggaat ttaaaacagc gtattttagg agatagagga catctttcca
    30781 acgaagacgg tgcacttgtc atgactgatg tgatcggtaa tcggaccaaa cgaatctatc
    30841 ttggccatct tagcaaggaa aacaatatga aagaacttgc tcatttaaca atggaaaatg
    30901 ttttgaaaga aaaagatttt ggcgttggac atgattttga aatctatgat acagatccag
    30961 atgaagcaac tgagctattt tcgatttaaa cagttcaatc aataaaaata taaaaagcag
    31021 aagataaaat caatcagggt tggttttatc ttctattttt atttttttag catattaaaa
    31081 aaataaccgt attatcgact gactacactg atgtgctgct tttcatgttt ctcatccagt
    31141 atctcatcta ctaatgcaat cgcgtaatct gccatgctga tcgtactgtc gccagcagcg
    31201 tttctttgta aataatcatc acttaacaaa taagtacctg tacgtttgcc atgtggatca
    31261 aataaagaag ctggactgat ataagtccaa tgtagatttt tagattgttt caattttaac
    31321 aatccttcgt acatattgaa agctgttgga tagaagggtg catttttagg tacttggtca
    31381 atcatacgag tcgtcttttc tgtatctaag aataacgaag aggcaccccc aacgacaatt
    31441 aagcgtgtat cggttccttc aagcatctca atcaaatggg acaaagaagt ttgatgcatt
    31501 tcttcttttc ctttgggagc gttgaaagca tcaacgacaa catcaaaaga tgcaagatca
    31561 gtagttgtca actcaaataa atccttttgt ataaacggaa catctgatgg caatttttct
    31621 ggatgacgca cgattgccgt cacgtctaaa tctctggaca atgcttcgtc taaaataaat
    31681 gaacctgcat ggccagtagc tccaatgagt gcaattttca taaaaaacct ccttttttat
    31741 tcctgtccat tgtaagcagt tgctgaatat tcgtcaaaga atccgatttt tgaaaagctc
    31801 agtgcgcatt cctttcatgg ttcgtgatca ttgggtatag ttaaattaag aaaactgatt
    31861 gataaaaata gaagttgaag gagataagaa atgaaagagt ggttagatcg tgccttttta
    31921 ccaatgataa catctgttgt gaatcttaga cccttaactg ctttgcgtaa tggaatgact
    31981 tacacgatcc caataattat tgttggctcg ctgtttttct tatttgctca ttttcctgtg
    32041 gattacatcg ctcagtggat agacaaaaca ggattttcta tgtattttac acaagtatac
    32101 caggctacat ttgatgtgat agccttttgg atcgtttttg ccattgcata cgcttatgca
    32161 aaagaagaag acatcgatcg cttaccagtc ggcataacag ccttgtctgc ttatctactg
    32221 cttattcagc cactaaatac tcagatagat actgtctgga caggtgctga agggatacta
    32281 ggagcagtta caactgggct catcatcgga tggggctatg cacgtttagt aagacttgcg
    32341 aaagggtgga agcaaagaga gaagatacct gaaaatgttt ggcagtcttt tacttcattg
    32401 attccaatca ttgttatagt cacttttacg cttatcggtt cagcggtttt tcaacagttg
    32461 ttcagaatga ctgttgttca aggagtatgg acatatgtcc agcttcccat tcaatcgttc
    32521 atagacacgt tgtttggtgt gcttttatta ggtttttttg ttccgttttt atggttattt
    32581 ggtcttcatg gatctgcgat ggtcaatggg ttggttagtc ccgtcttgca agcgaattca
    32641 ttagcgaatg cagaaatctt agcctcagga caagagttga cggtggcaaa tggcggacat
    32701 atcgttactc agcaatttct cgatcagttc atgacggtta cgggtgctgg attgacgtta
    32761 ggggctgtat tttttatgac ggtttttgcg aaatcgaaga aatacagaga attaggaaat
    32821 cttgctcttt taccagcctt tttcaacatc aatgaatcaa ttattttttc cacaccgatc
    32881 gtgatgaacc cgatgatggc tgttcctttt attttcgcac cggtcgtttc aggattgatt
    32941 acctattccg ctttatattt tggattcgtt cctttattta cagcagtcca agtcccttgg
    33001 acaacaccgc cgatcctctc gggtttctta gtaggcggag tgcctgcagc gattttacaa
    33061 ggtatcgtgt tgtcaattag ttttttcatt tatttcccat ttttgaaaaa aatagattct
    33121 gcaaactgca aaaaggaaag acagacaaaa aatgatggag cagcagaaaa gatgaacgac
    33181 taaaaaacaa aaatactcct gtcgctttag attgggaaaa tcaccaatct aggatgacag
    33241 gagtatttat agtcatgttt atactagccc tttttggaca gctaattgaa caagaacaac
    33301 taatgtgttc attcctacgt gtgtgatcat cgatgtccag atccgaccag ttgctttgta
    33361 ctctaggctg aagaagaaac ctaggaaaaa gtagattagc agatgaccat cattatgagc
    33421 aaacgcaaac agcaaagagc tgatcactgc tccgacccaa aaatttgaat atcgactgat
    33481 gatccccagt atactgcggc ggaatacgaa ctcttccatg atgggtccag caacagttgt
    33541 cgccaaaata aaagcgggtg cttccatgat catctggatg atattttgtg tattctcgga
    33601 aggggttgct ttgccaaaca atacagattc taacatgaca gcgatgcttt gcagaatcaa
    33661 tgcgacaaag attccaacca atccataaat aacaatcttt ggagcagaag ctttttttac
    33721 cgctgattcg atcgctaagg gttcacgttg cttaaaataa aaaatagcta ggacaacggc
    33781 gcctagtata tacatgactg cagttgctgt gatcaattca gacgtagatt ttacaagtcc
    33841 tatcgaagct aggatcaagg gtgagataaa aattagacca tagcaaaaaa tgctgaggaa
    33901 gctatatttt tttaaagaca tgtaattcct ccttttaagg gataacaagc atgcaaggca
    33961 agcttatatc gattgatttc aattatatca taatttcaat aagcagctgt cagaataaaa
    34021 tttcttttct ttgatttaaa agcttgcaaa aaagagggag aatgagtatt attaaaaatg
    34081 taggttagca ctcgaaggtt aagagtgcta acagattaaa aaaagagtta tcggagggat
    34141 ttatcgtgtt aaaaccatta ggtgatcgcg tcattatcga agtcgcaaaa gaagaagaaa
    34201 aaactgtcgg aggaatcgtt ttagcttctg ctgctaaaga gaaaccccaa actgggacag
    34261 ttgttgcagt cggtgaaggc cgtttgttag aaaacggtga aaaagttcct gccgctgtaa
    34321 aagcaggcga ccaagttatg ttcgaaaaat acgccggtac agaagtaaaa tacgaaggaa
    34381 aagaatatct gatcgtcgca ggaaaagata tcatggcgat tgttgagtaa atttggaaga
    34441 taaaaaaata atcaaaaata atcaatgagg tgaatcaatc atggcaaaag aattgaaatt
    34501 tgcagaagac gcacgcgcag ctatgttacg cggggtagat aaattagcag atacagtgaa
    34561 agtaacatta ggacctaaag gccgaaatgt cgtactagaa aaatcttacg gctctccatt
    34621 gatcacaaat gatggtgtga cgatcgcaaa agaaatcgaa ttagaagatc attttgaaaa
    34681 catgggtgca aaacttgtat cagaagtagc atcaaaaaca aatgatattg caggagacgg
    34741 tacaacgaca gcaactgttc tgacacaagc aatcgttcgc gaaggtttaa aaaacgtaac
    34801 tgcaggtgct aatcctctag gcattcgtcg cgggatcgaa ttggcaacaa aagcagcagt
    34861 cgaagaattg cataatatct caactgttgt tgattcaaaa gaagccattg cacaagtagc
    34921 agctgtttct tcgggttctg ataaagttgg acatttgatt gctgatgcaa tggaaaaagt
    34981 tggaaacgac ggtgtcatca caatcgaaga atcaaaaggg atcgaaacag aactagatgt
    35041 tgtagaaggt atgcagtttg atcgtggtta cttatcacaa tatatggtaa cagataacga
    35101 caaaatggaa gctgttctag aaaacccata tatcttgatc acagacaaaa aaatctctaa
    35161 catccaagat attctgccat tgttagaaca aatcttgcaa caatcacgtc cattgttgat
    35221 tattgctgac gatgtagatg gtgaagcatt gccaactctt gtattgaaca aaatccgtgg
    35281 aacattcaac gtagtagctg taaaagctcc tggtttcggc gatcgtcgta aagcaatgct
    35341 tgaagatatc gctattttga caggtggtac agtaattact gacgatctag gtttagaatt
    35401 gaaagatgta acaatcgaaa acttaggaaa tgcttctaaa gttgttgtag ataaagacaa
    35461 tacaacgatc gtagaaggtt ctggagaaaa agaagcaatc gaagctcgtg tgcaattgat
    35521 caaaaaccaa attgctgaaa caacttctga ttttgaccgc gaaaaattac aagaacgctt
    35581 agctaaatta gcaggtggtg tagctgttgt taaagttggt gcagctactg aaacagaatt
    35641 gaaagaatta aaattacgca ttgaagatgc attgaatgct acacgtgccg cagtagaaga
    35701 aggaatggtc tcaggtggtg gtacagctct agtgaacgtt atcagcaaag tttctgctgt
    35761 agaagcagaa ggagacgtgg caacaggtat caagatcgtt gttcgtgctt tagaagaacc
    35821 aattcgtcaa atcgctgaaa acgctggtta tgaaggatca gtgattgttg ataaattgaa
    35881 gaacgtggaa ctaggcactg gtttcaatgc tgctactgga gaatgggtaa acatggttga
    35941 agcaggtatc gtagacccaa ctaaagtaac acgttctgcc ttgcaaaatg ccgcttccgt
    36001 ttctgccttg ttattaacga cagaagcagt cgttgcagat aaaccagaac cagcagcgcc
    36061 agcagctcct gcaatggatc catcaatgat gggcggtatg atgtaagaaa aagcaacttg
    36121 cttatctaca actgatcatt agtgaaagac agataaaaag aggctggaac agaagcgttt
    36181 aactccaaga aataagaaga aattcacgga aattgctttt caaatttttg tgaatttcag
    36241 cttatttccg aaggagttgc ttctgcttcc gctgtttata cgtgtttaga gcgtgagaca
    36301 aaagtgttct ttacttttgt cccacgctct tttttgtgtg taattataat gaaaatagtt
    36361 atttttaata gttataatat tttatctatt aaaagtaaat atccattc
//
LOCUS       DACDYI010000021        36230 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.021, whole genome shotgun sequence.
ACCESSION   DACDYI010000021 DACDYI010000000
VERSION     DACDYI010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.021"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            232..1500
                     /locus_tag="IWM52_001021"
     CDS             232..1500
                     /locus_tag="IWM52_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HAP9973289.1"
                     /translation="MGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKSVEEIPSPVT
                     GTVKNVIVPEGTVANVGDVLVEIDAPGHNSASSTSAPSAEAPKEKVETSGSASVVEAA
                     DPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPSSAPAKSEAP
                     EAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVNSKHTAPHVT
                     LHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLNASIDDANQE
                     IVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDGKLSAEDMRN
                     GTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGRVMKLSLSFD
                     HRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1508..2914
                     /gene="lpdA"
                     /locus_tag="IWM52_001022"
     CDS             1508..2914
                     /gene="lpdA"
                     /locus_tag="IWM52_001022"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAP9973290.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            3076..3351
                     /locus_tag="IWM52_001023"
     CDS             3076..3351
                     /locus_tag="IWM52_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAP9973291.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3357..4124
                     /locus_tag="IWM52_001024"
     CDS             3357..4124
                     /locus_tag="IWM52_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAP9973292.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            4292..6121
                     /gene="typA"
                     /locus_tag="IWM52_001025"
     CDS             4292..6121
                     /gene="typA"
                     /locus_tag="IWM52_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAP9973293.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6382..8154
                     /gene="recQ"
                     /locus_tag="IWM52_001026"
     CDS             6382..8154
                     /gene="recQ"
                     /locus_tag="IWM52_001026"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAP9973294.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            8268..8570
                     /locus_tag="IWM52_001027"
     CDS             8268..8570
                     /locus_tag="IWM52_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAP9973295.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8573..9736
                     /locus_tag="IWM52_001028"
     CDS             8573..9736
                     /locus_tag="IWM52_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP9973296.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9762..13190
                     /locus_tag="IWM52_001029"
     CDS             9762..13190
                     /locus_tag="IWM52_001029"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAP9973297.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            13211..14335
                     /locus_tag="IWM52_001030"
     CDS             13211..14335
                     /locus_tag="IWM52_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973298.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            14325..14762
                     /locus_tag="IWM52_001031"
     CDS             14325..14762
                     /locus_tag="IWM52_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP9973299.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14746..15084
                     /locus_tag="IWM52_001032"
     CDS             14746..15084
                     /locus_tag="IWM52_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAP9973300.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            15203..15763
                     /gene="rsmD"
                     /locus_tag="IWM52_001033"
     CDS             15203..15763
                     /gene="rsmD"
                     /locus_tag="IWM52_001033"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAP9973301.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15760..16251
                     /gene="coaD"
                     /locus_tag="IWM52_001034"
     CDS             15760..16251
                     /gene="coaD"
                     /locus_tag="IWM52_001034"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAP9973302.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            16241..17284
                     /locus_tag="IWM52_001035"
     CDS             16241..17284
                     /locus_tag="IWM52_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP9973303.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17443..18171
                     /locus_tag="IWM52_001036"
     CDS             17443..18171
                     /locus_tag="IWM52_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAP9973304.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            18287..18532
                     /locus_tag="IWM52_001037"
     CDS             18287..18532
                     /locus_tag="IWM52_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP9973305.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18529..18747
                     /locus_tag="IWM52_001038"
     CDS             18529..18747
                     /locus_tag="IWM52_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP9973306.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18883..19422
                     /locus_tag="IWM52_001039"
     CDS             18883..19422
                     /locus_tag="IWM52_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAP9973307.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19415..21688
                     /locus_tag="IWM52_001040"
     CDS             19415..21688
                     /locus_tag="IWM52_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAP9973308.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21726..22772
                     /gene="holA"
                     /locus_tag="IWM52_001041"
     CDS             21726..22772
                     /gene="holA"
                     /locus_tag="IWM52_001041"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAP9973309.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22861..23115)
                     /locus_tag="IWM52_001042"
     CDS             complement(22861..23115)
                     /locus_tag="IWM52_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAP9973310.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23515..23784)
                     /locus_tag="IWM52_001043"
     CDS             complement(23515..23784)
                     /locus_tag="IWM52_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973311.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(24254..24709)
                     /locus_tag="IWM52_001044"
     CDS             complement(24254..24709)
                     /locus_tag="IWM52_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAP9973312.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24847..26775)
                     /locus_tag="IWM52_001045"
     CDS             complement(24847..26775)
                     /locus_tag="IWM52_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP9973313.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(26972..27805)
                     /locus_tag="IWM52_001046"
     CDS             complement(26972..27805)
                     /locus_tag="IWM52_001046"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP9973314.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(27997..28701)
                     /locus_tag="IWM52_001047"
     CDS             complement(27997..28701)
                     /locus_tag="IWM52_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9973315.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(28724..29350)
                     /locus_tag="IWM52_001048"
     CDS             complement(28724..29350)
                     /locus_tag="IWM52_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9973316.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            29676..32051
                     /locus_tag="IWM52_001049"
     CDS             29676..32051
                     /locus_tag="IWM52_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAP9973317.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(32075..32566)
                     /locus_tag="IWM52_001050"
     CDS             complement(32075..32566)
                     /locus_tag="IWM52_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAP9973318.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            32753..33673
                     /locus_tag="IWM52_001051"
     CDS             32753..33673
                     /locus_tag="IWM52_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9973319.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            33827..34171
                     /locus_tag="IWM52_001052"
     CDS             33827..34171
                     /locus_tag="IWM52_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAP9973320.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(34361..34861)
                     /locus_tag="IWM52_001053"
     CDS             complement(34361..34861)
                     /locus_tag="IWM52_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9973321.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            35389..35472
                     /locus_tag="IWM52_001054"
     tRNA            35389..35472
                     /locus_tag="IWM52_001054"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35423..35425,aa:Leu,seq:cag)
     gene            complement(35796..>36230)
                     /locus_tag="IWM52_001055"
     CDS             complement(35796..>36230)
                     /locus_tag="IWM52_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAP9973322.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        11990 a         6325 c         7491 g        10424 t
ORIGIN      
        1 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
       61 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
      121 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
      181 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agttaccgga tatcggtgaa
      241 ggaatcgcag aaggtgaaat cgtcaaatgg ttcgtcaaac caggagatac gatcaatgaa
      301 gatgatacat tactagaagt tcaaaatgac aaatcagtag aagaaattcc gtcacctgtc
      361 acagggacag ttaaaaacgt gattgttcca gaaggcactg tagcaaatgt tggggatgta
      421 ttagtagaaa tcgatgctcc tggacacaat agtgcatcgt caactagtgc gccatcagct
      481 gaagcaccaa aagaaaaagt ggaaacatca ggttcagcaa gtgtcgttga agctgccgat
      541 ccaaataaac gtgtattggc tatgccatct gttcgtcaat ttgcccgtga aaaagatgta
      601 gatatctcac aagtaactgc aactggaaaa ggtggcagag taacaaaaga ggacatcgaa
      661 aacttcttgg caggtggtcc aagcagtgca ccagcgaaat cagaagcgcc agaagcggca
      721 gcaccgaaag aagcagctcc tgcagcagaa agcaaaccag ctgcaccagc aaaaccattc
      781 aagtccaact taggagactt ggaagaacgt gtagcaatga cgccaacacg taaagctatc
      841 gctaaagcaa tggtcaacag taagcataca gctccacacg tgacattgca tgatgaagta
      901 gaagtgtcta aactatggga taatcggaaa cgatttaaag aagttgctgc tgcaaatggt
      961 acgaaactta ctttcttgcc atatgtcgtg aaagctttaa cagcgactgt taagaaatac
     1021 ccagtattga atgcatctat cgacgatgca aaccaagaaa tcgtttataa acattactac
     1081 aatatcggta ttgcaactga tacggatcac ggactatatg taccaaatgt gaaagatgca
     1141 gaccgcaaag gaatgtttgc tattgcagac gaaatcaatg agaaagcaaa attagctcat
     1201 gacggcaaac tatctgcaga agatatgcgc aatggaacaa ttacgatcag caatatcgga
     1261 tctgtaggtg gcggttggtt tacaccagtc atcaactatc cagaagtagc tattttaggt
     1321 gtaggaacga ttgcacaaca accaatcgta aatgctgaag gcgaaatcgt tgtcggtcgt
     1381 gtgatgaaac tgtcattaag tttcgatcac cgtatcgtag atggagcaac tgcgcaacaa
     1441 gcaatgaata atatcaaacg tcttttagct gatccagagc tattaatgat ggaaggatga
     1501 taaacaaatg gtagttggag attttgctgt agaattagat acagttgtaa ttggtgcggg
     1561 acctggtggc tatgtagctg ccatccgcgc tgctgaaatg ggacaaaaag tagcaatcat
     1621 tgaaagagag tatattggcg gtgtttgttt gaatgtcggc tgtattcctt caaaagcact
     1681 gatagctgca ggacaccatt atcaagagtc actagattct gaaatgtttg gagtaactag
     1741 tgaaaacgtc aaactagatt ttgcaaaaac acaagaatgg aaagaaaaca aagttgtcca
     1801 tacgttgact tctggtgttg gtttcctatt aaaaaaacat aaggttgaaa caatcgaagg
     1861 agaagcgttc ttcgtagatg atcacacact acgcgtcatt catccagatt cagctcaaac
     1921 ttattcgttc aataacgcaa tcatcgcaac aggttcccgt ccaatcgaga ttccaggatt
     1981 taaattcgga ggacgtgtgt tagattctac tggcggatta gcattgaaag aagtacctaa
     2041 gaaatttgtg atcatcggtg gcggtgtgat tggtgccgaa ttgggcggag cgtacgcaaa
     2101 cttaggagca gaagtaacga ttcttgaagg ctcaccacaa attttaccaa cttacgaaaa
     2161 agatctagta aaattagtag aagatgattt caagaaaaaa ggcgtaacag tcgtaacgaa
     2221 tgcaatggcg aaagaagcag tcgataacgg tgacagcgtg acagtgaaat atgcagtgga
     2281 cgggaaagaa gaatccgtta ctgctgatta tgtgatggtc actgtaggtc gtcgtccgaa
     2341 tacagatgat atgggcttag aacaagcggg tgtcgaagta ggagaacgag gattgatcac
     2401 ggtggataaa caaggacgta cgaacgtacc taacatctat gccatcggtg atatcgttcc
     2461 aggggcggca ttagcacaca aagccagtta tgaagcaaaa atcgctgctg aagcaatctc
     2521 tggcaaaaaa gtagctgtag attataaagc aatgccggca gtagccttca ctgatcctga
     2581 attggcttct gtaggtatga cgatcaaaga agcaaaagat gctggtatcg aagcaaaagc
     2641 atataaattc ccattttcag ggaacggtcg ggcattatcg cttggtaaaa cagaaggatt
     2701 catccgtcta gttacgacga tcgaagataa cgtgcttatt ggcgcgcaaa tcggtggagt
     2761 cggcgcaagt gatatggtct ctgaattagc tttagcaatc gaatcaggaa tgaatgctga
     2821 agatatcgcc ttgacgattc atcctcatcc atctcttggc gaaatcgtca tggatgcttc
     2881 ggaattagca ttaggtttac caatccatat ttgatttgaa taatacaggc gaaacgcaac
     2941 tgaaaaatca gttgcgtttt gttttttaga attatcaata atattattat gtaaacttaa
     3001 aaatatgaca acttcttggt ttattaccac ttcctatgct acactgaaga ggaatatgaa
     3061 ataggagagt tgaagatgca agaatatcag tatccacttg atttagattg gacaacagac
     3121 gaaatggtca ttgtaatgaa gatgtgggaa gatctggaac gtgcgaatga aaaagggata
     3181 aacaatgaag cttttttgaa gacttatcag cagtttaaaa ctgttgtcaa atccattggt
     3241 gaggaaaaac gattgggacg ggaatttgaa aaagcttccg gttattcttt atatcgtaca
     3301 gtgcagcaag caaaaaaaaa tccaaacaaa actttgagaa tgaacgggtg agataaatgg
     3361 aacagatgat tgatcatatc aagtcctggc ttgaagaagc tggtgagaaa atcaaatctt
     3421 ctcaacaaac attaacaatc tcgcaaaaat caaatcgaac ggatctagta acaaatgtcg
     3481 atcgaaacat ccagaatttt ctaatcgaaa aaatccattc ctttgatcca gaggcgaaaa
     3541 tattggcaga agaagaaggg tgtaatcaat tatcggatct cgatggaaga gtctttatca
     3601 tcgatcctat tgatggcacc ttgaattttg tgattgaagg ggaaaatttt tgtattatgc
     3661 tcgctgttta tgaatccggc gttggaaagc ttggtttcat ttatgacgtg atgagaaatg
     3721 aacttttttg gggtggaaaa ggaataggcg tgtataaaaa tgatgagcca ttgcctagac
     3781 cagcaaataa atccttatcg gaaggcttac ttggtgtgaa tagttatctt ttcggtcata
     3841 ataaatatgg aatccgagaa attgcggaaa gcagcatggg gacacgtatg tacggatgcg
     3901 ctggtcttga actagtggca atcttgaaag gaaaccacat tgcttatatt tcaaacctta
     3961 gcccttggga ttatgcagcg ggaaatgtct tattggaaga gtttggtatg gtaacaagcg
     4021 gtctttttgg tactcctttg acatttgaaa aaagggaata ctatttagca gggacttctt
     4081 gtgcatatgc agaaatcgca gaccttttga aaaaagaagg ctgaaaaaaa ggtagaaatc
     4141 attcacaaaa atgataaaaa cgagcaaaag attaattttc tcgaaaaaat gaaaacattt
     4201 tactatattt catgaaaaaa gtctgctata attagcagtc gagtaaaatt tgccctgtca
     4261 taaagaacca ataaaaaagg agttagttac attgaattat agaaacgata tccgtaacgt
     4321 cgcaattatt gcccacgttg accacggaaa aacaacatta gttgatgaac tattaaaaca
     4381 atcagataca ttagacgcac atacgcaatt gcaagaacgt gcgatggact caaatgcgct
     4441 agaaaaagaa cgtggtatca cgattttagc caaaaacaca gctgtagact ataaaggtat
     4501 tcgcgtaaac atcatggata ctccaggaca cgcggacttc ggtggcgaag tagaacggat
     4561 catgaaaatg gttgacggcg ttgtgttggt cgtagatgcg tatgaaggaa caatgcctca
     4621 aacacgtttt gtattgaaaa aagcactgga acaacatatc acaccaatcg ttgtcgtaaa
     4681 caagattgac aaaccttctg ctcgtcctga gcatgtcgta gatgaagttc ttgaactatt
     4741 catcgaatta ggagcagatg atgatcagtt agactttcct gtgatttatg catctgcact
     4801 aaatggtacg tcaagcttgt cagatgatcc agcagatcaa gaaccaacca tggcacctat
     4861 ttttgataca attattgaaa aaatccctgc tcctgtcgat aacagcgacg aaccattaca
     4921 attccaagtt tcattacttg actacaacga ttatgtaggt cgtatcggaa tcggacgtgt
     4981 atttagagga acaatcaaag taggagacca agtagcatta attaaattag acggaacagt
     5041 taagaaattc cgtgtaacga aattatttgg tttctttgga ctaaaacgtc tagagattca
     5101 agaagcaaaa gctggagact tgattgctgt ctcaggtatg gaagatatct ttgtcggtga
     5161 aacagttact cctgtggatc atcaagatgc gttacctatc cttcacattg acgaaccaac
     5221 attgcaaatg actttcttag ttaataattc accatttgcc ggtcgagaag gtaaattcgt
     5281 aaccgctcgt aagattgaag aacgtttgat ggcagaactt caaacagatg tatccttacg
     5341 agttgaacca acaaactctc cagatgcatg gacagtttct ggtcgtggag aattgcattt
     5401 gtctatcttg attgaaaaca tgcgccgtga gggatacgaa ttgcaagtat ctcgtccaga
     5461 agttattgaa aaagaaatcg acggtgtaaa atgcgaaccg tttgaacgcg tgcaaatcga
     5521 tacaccagaa gaatatatgg gtagtgttat cgagtccttg agcttacgta aaggtgaaat
     5581 gcaagatatg gtccatacag gaaacggaca aatccgtttg acattcttaa cacctgcccg
     5641 cggtttgatt ggttattcaa ctgaattcct ttcaatgaca cgtggttacg gaatcatgaa
     5701 ccatactttt gaccaatatc tgccaatgct tcctggtcaa attggcggac gtcaccaagg
     5761 ggcgcttgtt tcgatcgata caggaaaagc aacgacttat tctatcatga gtatcgaaga
     5821 acgcggtaca gtcttcgtag aaccaggtac agaagtatat gaaggaatga ttatcgggga
     5881 aaacagccgt gataatgatt tgactgtcaa catcacgaaa gcaaaacaaa tgacaaacgt
     5941 acggtctgct actaaagacc aaacatctgt catcaaaaaa ccaaaacaat tgacgttgga
     6001 agaatctttg gaattcttga atgatgatga atattgcgaa gtaacaccag aaagcattcg
     6061 cttgagaaaa caaattttag agaaaaatgc tcgtgaaaaa gcatctaaaa agaaaaaata
     6121 atctgtaaaa ggatgaagag agaaaccagc ttgttataaa aaatagctgg tttctttttt
     6181 tgtccaactc ttttctgtgg agaagtcatc tttgtcaatg gacataaatt cagaaaatac
     6241 gccgaaagac gtttaaaaga cttttaaaaa acttatttct agagagtttt tgtttcataa
     6301 taagataatg tttcacgaaa ggagatcaac ttgtccttcg aagaaaatcg aagtaaaatg
     6361 gcaagagaaa ggagcgggaa gatgaacgac ttattaaaac aatactttgg ttatgatgag
     6421 tttagacctg gtcaaaaaga aattatccag aaagtcattg atcaggaaaa tgtattagga
     6481 atcatgccta ccggaagtgg gaaatcaatc tgttaccagc ttccagcttt actattagat
     6541 gggcttactg tggttgtgtc ccctttaatc tctttgatga aggatcaggt ggacgcggcg
     6601 aatcaattag gtatacccgc cacttttatc aatagctcgt tagatggtta cgaaacagct
     6661 agacgcttcc aagaaatcga tagacagcag taccgtttac tttatatcgc tcctgaacgt
     6721 tttatcatgc cggattttat ccaagcaatg aagcgatgga acgtccgtat gatcgctatt
     6781 gacgaggcac attgtatctc tcagtggggg catgatttcc gcccaagtta tttacagatg
     6841 gcgaaccagc tagatcaact gccaaatcgt cctgtaattg ttgctttgac agctacagcg
     6901 actgtgcaag tagctgctga cataaaaaga cttttaaaaa ttccagaaaa caaccatatt
     6961 cagactggtt ttgaacgaga aaatctacga tttcaagtaa ttaaagatca aaaaaaagaa
     7021 caatatctaa tcgaatattt aaagatcaac aaaaatcaat ctggtatcat ttatgcagca
     7081 acgagaaaag aagtagaccg tctctatcat ttgcttaaaa aattcgattt ttccgtgggt
     7141 agatatcatg gtggtctaaa tgagaatgag cgaacagaaa tgcaagaagc ctttttatac
     7201 gatcgcttgc agctgattgt agcgacaaat gcatttggta tgggtataaa taaaagcaat
     7261 gtccgttttg ttatccatta tcaaattcct ggctctctag aagcatacta tcaggaagct
     7321 ggtcgggcgg gaagagatgg tctttccagt gaagccatcc tgcttttctc tcctcaagat
     7381 atccaagtcc aaaagttttt tatccagcag tcccaaagag aagaagggca aaagcaaaaa
     7441 gaatatgaaa aattaaaagc gatgacagaa tacgtgtata ttgagtcctg tcttcagcaa
     7501 tacatcctaa actattttgg cgagacaagc agtccgtgca accgttgtgg taactgttta
     7561 gatgacagag aaatagtaga agtaacgaca gaagcacaga tggttctttc ttgcttaaaa
     7621 cgtatgggtg agaactatgg taaacaaatg ctgatgaaag tcttagcagg atcgaaagaa
     7681 caaaagctgc aagctttagg ttttggacat ttatctacct atggactgat gaaaaaccaa
     7741 tcgcaaaaag aaacgatgca gttaattgaa tatttgatat cgaatggcta tcttctgacc
     7801 atcaatggtg agtatcctgt tttgaaagta acagaaagag ggatacaagt attgaaagga
     7861 caagaatctg tttatcgaaa ggaacccaag aaagtccagc agctatcgga tgaagaaacc
     7921 gatacactat ttgaagtgct gcgggaactg cgaacggatc tagcatcaga agcaggcgtt
     7981 cccccgtatg tcgtattttc agattccaca ttgaaagaaa tgagccgaat acgtccatca
     8041 agcagattgg agatgcttca gataaaagga gttggccaaa gcaagcttga taaatatggc
     8101 gaagcctttt tatctcggat aaaaaatgcg gactctaacg ttctggataa gtagaaagaa
     8161 cgaatacgta aaaatgaata aaaacaactg gttaaatctg ttttttttgc tccttatgaa
     8221 aatcaaatat gttataatta aggaattaga atgggaggta agcacagatg gattctttgt
     8281 cgaaaaaact tgcgatccaa gtattaaatg atgaagcaga acggatcaaa cgattaatcc
     8341 gaaatcaaaa caacagtctc tgtatctcac aatgcaaagc cttcgaagaa gtggtagata
     8401 cacagatgta cggcttttcc cggcaagtcg gttttgctgt ccgtatagga gtaatcggaa
     8461 agaacgaggg ccagctgatg ttaagtgagc tagaacgaga attgaatcag ttatatagtg
     8521 aggtttacca agaaatttat gataaaaaag aagttggcaa ggaggaatag acttgcctaa
     8581 gaaagtgaaa aaaaggcatc tattagacta tagcatcttg attccctatt taattttatg
     8641 tgtattggga ttgatcatgg tatatagttc tacgtcgtat ctattattag aaaacggaca
     8701 aaatccgtcc gcatcagtca ttaaccagag tattttttgg gtgcttagct tgattgctat
     8761 cgcactactt tataaaatga aaacagatgt tttgaaaaac cagcgtttga tcatggcagc
     8821 catcgcagtt ttgacgattt tgctgctgat tgttgtgttt tttggtaagg agatcaatgg
     8881 ggcgaaaggt tggctgcaaa ttgcaggatt ctcgatccaa cctgccgaat acctaaaaat
     8941 catttctatc tggtacctgt ctctgacttt atctaaaaga caaaacagtg tacaaaaaga
     9001 ttttcttggg actgtgaaac gtccacttgc aatggttatc ggacttactg cattagtcgc
     9061 tattcttccc gattttggta atgcggcagt tatctttttg attattttgg ttctgctttt
     9121 agccagcgga gtcaattatg tgtatacttt gattgtagga gtaggaggct tttgtctaag
     9181 tacttttacg atctggctga tcaatatcac aaacggaaag atcttacccg gacgtttaca
     9241 gtatatctat aatcgatttg ctatttacca aaatcctttc tcagacgaat taaataaagg
     9301 gcatcaacta gtcaacggct actatgccat gttcaacgga ggactatttg gcaggggatt
     9361 aggtaacagt atccagaaaa aaggattttt acaagaagca caaaccgatt ttatttatgc
     9421 aatcgtagtg gaagaactgg gagtgattat ggggattttg atattagcgt tgctattctt
     9481 tatgattgtg cgcattgtcc ttgtaggtat ccgttcaaaa gatccgttca attcgctttt
     9541 gtgcattggg atcggcgcaa tgtttttgat ccaagtattt gtcaatcttg gaggaattac
     9601 tggcgttatt cctttgacag ggatcacgtt cccattttta agtcaaggag gctccagttt
     9661 gctgatgctt tcgatttgtg tcggctttgt cctcaacatc agtgcggatg aaaaacgcaa
     9721 gagcttaggt ttataataaa aaaagatgta ggagtggttc gatgaaaaaa gttttagttg
     9781 ctaacagggg tgaaatcgct gtacgtgtat ttcgtgcttg tacagaatta ggaattaaaa
     9841 ctgttggaat ctatgcagaa gaagatgaat attccgtaca ccgtttcaaa gcggatgaag
     9901 catatttagt aggacaagga aaaaaaccga ttgatgcgta tctagatatt gaagggatca
     9961 tttctatcgc gaaagaatgc ggagctgacg ctattcatcc gggttacggt cttttgtcgg
    10021 aaaacttgaa ttttgcgcaa cggtgcgaag aagaaggcat catttttgtt ggacccaaac
    10081 tgcatcattt agatattttt ggagataaaa tcaaagcaaa agctgcagct atcgaagcgg
    10141 ggattgcttc gattccaggc acagatggac caatcgcttc tatagacgat gccttggagt
    10201 ttgcgaaaca atacggttat ccgattatga tcaaggcagc tctaggcggc ggcggacgcg
    10261 gcatgcgtgt agctcacgat gaaaagagtg caagagaggg ctacgaacgt gcgaaaagcg
    10321 aagcaaaagc agcctttggt agtgatgaag tgtatgttga aaaatacatc gctaatccta
    10381 agcatatcga agtacagatt ctcggggata cgcatggaaa cgtcatccat ctttttgaaa
    10441 gagattgttc tgttcagcgc cgtcaccaaa aagtagtaga agtagcgcct tgtgtctcta
    10501 tgaatgaaca gcaacgtcaa aaaatctgcc aggcagctgt ccaattgatg aaatatgtcg
    10561 ggtatgtcaa cgcaggtaca gtagaatttc ttgttgaagg agatgatttt tactttatcg
    10621 aagtcaaccc tcgcgtccaa gtggaacata cgatcacaga aatgattaca gatattgata
    10681 tcgtaacaac gcaactgttg attgctcaag gtttggatct gcataaagaa atcggattgc
    10741 ctcagcaaga aggaattaaa ttaaatggtt cagccattca atgccgaatc actacagaag
    10801 acccgttgaa taacttttta ccagacacag ggaaaatcga tacgtatcgt tcacctggtg
    10861 gttttggcgt acgtctggat gtcggaaatg cttacgcggg ctatgtagtg acaccgtatt
    10921 ttgattcatt gctagtaaaa gtatgtacgc atggtgctac ctttgaaaca gccattcaga
    10981 aaatggaacg ttgcttgaaa gaatttagaa tccgtggagt gaaaacaaac attccattta
    11041 tgctcaatgt catcacacat cctgaattcc aatcaggaaa tgcaaaaaca accttcattg
    11101 atagtacagc aacattattt gaatttcctc gactacgaga ccgcggaaac aaaacgatga
    11161 aatatatcgg tgaaattact gtgaatggtt ttccaggtat cgaaagtggc gaaaaaccat
    11221 tttatgaaga accacgtatg cctaaagatc taatcacaag aactgattac gtaacggcta
    11281 agaatgtatt agatgcgaag ggtgccgatg ctttggtcga atggatcaaa ggacaggaaa
    11341 atttgttgtt gacggatacg acatttagag acgcccatca aagtctgcta gcaactcgtg
    11401 ttcggacaaa ggatttcaag cagattgcac gtttgactgg agaaggtctt cctgaactct
    11461 tttcaagtga gatgtggggg ggagcaacat ttgatgtggc ttaccgcttt ttgaatgaag
    11521 atccttggca acggttgaga aaaattcgtt ctttaatgcc taatacgttg ctgcaaatgc
    11581 ttttcagagg ttcaaatgcc gttgggtatt ctaattatcc tgacaatgta ttggtggaat
    11641 ttgtcaaaga agcagcagcg caaggaatcg atgtatttcg aatctttgat agtttgaact
    11701 ggactccaca aatggaaaaa agtatccaag ctgtacggga tactggtaaa atcgcagagg
    11761 cagctatttg ctatactggc gatatcaacg atccaagtcg ggcaaaatac aatgttcagt
    11821 attataaaga catggcaaag gaactagaac aactgggtgc gcatatcatt gcaatcaaag
    11881 atatggctgg gctgcttaaa ccgcaggcag cttatcgttt gattagtgaa ttgaaagaaa
    11941 cgacagatct gccaatccat ctgcatactc atgataccag cgggaatgga atcatcactt
    12001 attctgctgc ttcaaaagca ggagtagaca tcgttgacgt ggcaatgagt gcaatgagtg
    12061 ggaatactag tcagcctagc atgagcagct tgtattatgc actggtcaat ggtccacggc
    12121 tgccagaaat cacgattgaa aatgcacaga aattaaacca ttattgggaa gatgtgcgca
    12181 tgtattacaa accgtttgaa aacggcttga atgctcctga aacagaagta tatatgcatg
    12241 aaatgccagg aggacagtat tcgaatctac agcaacaggc aaaagctgtc ggattaggtc
    12301 atcgctggga tgaaatcaaa caaatgtatc atactgtcaa cttgatgttt ggcgatatcg
    12361 ttaaagtaac tccatcatct aaagtagttg gagatatggc actatttatg gtccaaaatg
    12421 atcttacaga agaagatatt tatgaaaaag gcgaaacact aagtttccct gaatctgttg
    12481 tgactttctt ccaaggagaa ctagggcaac ccgttggcgg atttcctaaa aaattgcaaa
    12541 agattattct taaaggcaga cctgcattga atgaacgtcc aggtcttttg gctgaatcag
    12601 tcgacttcaa cgaagtaaaa aaagaattgg cagaaaaaat tggctatgaa ccaaaacaag
    12661 acgaagtatt aagctatttg atgtatccgc aagtcttttt ggactatcaa aaagcatata
    12721 atcaatttgg cgatgttaca ttactggaca caccaacatt tttccaaggt atacgtttgg
    12781 gtgaaacaat caatgtacaa atcgaacgcg gaaaaacgct tatcattcgt ttagatgaaa
    12841 tcggagaacc cgatatcgaa gggaatcgcg tactgttctt caatttgaat ggacaaagac
    12901 gtgaaatcac tatcaaagat aattcgatca tcagtacggt ccaaacccga ttgaaagctg
    12961 aaccgacaaa ccgagaacaa attggagcaa cgatgtctgg ttctgttttg gatgtattag
    13021 tgaaaaaagg cgataacgtt aaaaaaggtg atacgttgat gattactgaa gcgatgaaaa
    13081 tggaaacggc catcgaagca cgttttgacg gagaagtcgc acatgtatat gtgtcttcag
    13141 gagacactat ttcgtctggt gaccttttga ttgaagtgac tgagaaataa taagcgaaag
    13201 gtggggaaac atgaaaagac tgggtttgtt ttggctgatt ctgctattag ttattgtggc
    13261 attctacatg gaaccagtct tgtttccgcc aaaacttgaa gtgaaggaaa actctgatca
    13321 acaaatatca aaccaaatga caacgtggaa ataccaggag cttaaagcaa ctggtttttc
    13381 tacttatatc ggaaaaccga tcgatcagct ggaaaaggat tttggccaag cctatgatcg
    13441 attaactagc ggatttggtt ttgaaacaag atattatgaa aataaagcta gtcatctcac
    13501 atttgaagcg aacatccaag acagcagagt cacaactgtt aaagtactgc aaacagggcc
    13561 tgctgatatc gcgccgtttt atttagggat gacgatgcaa gatcttacga aaatcactac
    13621 tatctatact aatttcactt ttgagtacca tgatacagaa gttggaatcg aactaatgga
    13681 agaggatatg aattatcgtc cgctaattgc ttttgataac gacacttttg ctattttatt
    13741 ttttgatcaa acaacaaacg gcctattttc agttgtgtat ttagataaag atagtctttt
    13801 gaaactattg ccatatcaag tgtttggtga aggccttcca cattatcaag cagataaatc
    13861 ggctgattgg gaatcaatca atcgcgttaa agaacgaaaa agtacgactt tattaaatat
    13921 gcttagaaga gaagacgaac ttcccatata caatcaaaca cttgcctttc aaaaccacag
    13981 tcagctgcta ctacatgatt ttttgacaag gcctgaggaa atattgtcag aggaacgtat
    14041 ggctgattgg caaaaatcgc taggcagtgc tcaaacgaca gtgaaattct cactgacgaa
    14101 tgatgaattg gagaagttaa tcagtaaaca aaaattagaa aatactagtg ggctatttat
    14161 tcatccggcg attgacgcta ctttttcgtt tttatattgg tacagcgatc cttacaacca
    14221 tgaccgtttc atggacccgg gaacggattc tgtaggcatt gctttttcta aagaaaatat
    14281 gttagtctta ttgcaagaag aaaacggaga aagtagtgat agcagtgatc gttaacgaag
    14341 agctgtttca actggaggat caatgcctcc aagtcgctga aatgataaaa aagagtaaaa
    14401 cgatggcttc ttatcttgaa cataaacagt caatggaaga atctgcaaaa gtttgccagt
    14461 tgaaagaagc gttcttgtct aagaaagaag catttgaaaa tgtagcggca tatggtgagt
    14521 atgcacctga ttatcgtgaa aaaaaacgag ctgttcgaaa agcaaaaaga cagcttgata
    14581 tggatgagca tgttgcagct ttccgatata gtgaaacaca gctgcaaagt ctgttggatc
    14641 aaattgtcca agtacttgcc gaagaagttt ctaatgaaat caaagtagat gcaggtagcc
    14701 cgttttttga aacgaaaaaa cactttggat gcggaggtaa ttgtcatgca attagacaat
    14761 agtgaatttg aaataacaaa acgacgcggg ctgatcgtat gggtctacag cttaaaacaa
    14821 ttaaagaatc tgaaaaagtt cggcttgttg cattatgtct caagaagaat gaaatatgtc
    14881 gtgctttact taaatgaaga gacattggaa gaaacagaag aacgaatccg taaattacat
    14941 tttgttcgaa acgtagaacg ttcttaccga ccggatatcg aaatgaattt tgccgaaaaa
    15001 ataggaaaca aaacagtatt aaaagacgaa ggtttcgaaa ttgaagagct aaatacagaa
    15061 atccgattag ctgaacatct ttaaaaatct cttatgagca gtttttaaat aaaaaataac
    15121 ttggataacg atcgattttt gatcattgtc caagtatttt tttggtatac tagtaccagt
    15181 accatttcta aggagtgagt agatgcggat aatatcagga aattatggcg ggcgccgttt
    15241 aaaaagttta gcaggggcga acaccagacc aactacagat aaagtaaaag aatccatatt
    15301 caatatgatt ggaccatatt ttgatggaga gacagtattg gatctgtttg caggaagcgg
    15361 tggtttagct atcgaagcag tatctagagg ctgcagtcat gcggtatgcg tagacaaaaa
    15421 ttaccaagcg ctaaaaatca tcaaagaaaa catcgaaatt acgaaagaac cagagaaatt
    15481 taccacaata aaacttgatg cggacaaagc aatcgaacgg ctagcagcgg agaaacaaca
    15541 attcgactac ttgtttcttg atccgccata tgcaaaacaa aaaataatcg atcaaatcca
    15601 taaaatggaa gaactgggac ttttttctaa aaccgcagtt atcgtatgcg aaacggataa
    15661 aacagtcgaa ttgccagaaa ctgtggctgg attccatcaa ataagaaaac agatttatgg
    15721 cataacggcc gtaactatct atagaaaaga gggaaatcaa tgaaacgtgc attattccca
    15781 ggaagttttg atccttttac aaaaggtcac ttagatacgg tggaacgtgc ggctaaatta
    15841 ttcgatgagg tagtaattgg tgtttttatc aatacttcaa agaaaagttt gtttccccca
    15901 gaagaaagaa tgacattgat cacaaaagct gtttctcatc ttcctaatgt aaaagtcatg
    15961 catcaagaaa atcaattgac ggtagaaaca gcaaaagaga ttggtgcaga tgcgttgatc
    16021 agaggcatac gaagtatcaa agactttgaa tatgaacgag agattgctca gatgaatcat
    16081 catctatatg atgaattgga aactgtcttc ttattagcaa aaccagaata tagtcacgtg
    16141 agttccagta ttttaaaaga agtactgcac tttggcggag atgtgagcag ctacttaccg
    16201 ccagtgatca acgaagcttt agctggaaag cgtgaatcaa atgaaagcta aacaatggtt
    16261 gaaaacagta ctgattatca tatttggaat gttgatcatt ggatttgtag tagttccctt
    16321 gccgtattat gtagaatcac cgggagccac gatcgatctg aaagaactaa ttacggtcaa
    16381 tgataaggaa gataaaggtt ctgggtcttt ctctcttacc tctgttggta tcaggcaagc
    16441 tacaggtttc ttagctttaa aagctaaaat gacccctttt gaagaattga tcagtgaaga
    16501 agaattgact ggtggggcga caaatgaaga atataatcaa atgcaacagt attatatgga
    16561 ttcttcccaa aatgcagcaa tcgaacaagc gttaaaactt gcagacaagc cttataaaat
    16621 ggaattcaaa ggcgtatatg tcatgagtgt agagaaaaca tcgaatttct ttggcaagct
    16681 ttcagttgga gatacagtaa caaaagtaga cgggaaaagc tttcaaagta cagaagcatt
    16741 tatggattat gtaaaaagcc aaaaagttgg acaaacaatc gaaatcagtt atttgcataa
    16801 tggtgaagaa aaaacagctt ctggtgatct gatcgagtta ccaacagata aaaaggcagg
    16861 aataggtatc acgcttactg accataccga gatcgaatca gatacagatg ttcgtattaa
    16921 ttctggctct atcggtgggc catcagcggg attgatgttc actttagaaa tctatgaaca
    16981 agtcactggt aaaaacttgc gacatggtaa gcaaattgca ggaactggaa cgatcaacag
    17041 tgaaggagaa gttggccgaa tcggaggaat cgataaaaaa gtggcaagtg cggataaagc
    17101 tggcgtagag attttctttg caccagatga tacgatttca gacgatgtta agaaagaata
    17161 tcctgaaata aaaaccaact acgaagaagc aaaagctgcc gctaaaaaat tgaattcctc
    17221 aatgaaaatc gtaccagtaa aaacagtaca agaagcgctt gactatctag agaaaatgaa
    17281 atgaaactaa ataattaaag aggggttgtg acacgagttc tgtcacaacc cctctttttc
    17341 gtcaaaaatc ttcacgataa aaataaaaac aaaagaaaaa attgacgtaa ttagatgttg
    17401 aagggacgat tttgccagag ctcactgttt cttcaatttt aaatgaaagg agtaatgaat
    17461 gatttttact ggtcattctt tgaaaaaaga acaatgatag aaaaaatcag caagaaaata
    17521 ctagcttcac ccaaattgat catttggatg tctatgatag ttcttatctt ttgtatcgca
    17581 agtggttttt tattatcaaa tttgctattt tatagagaaa cagaagagac tgctgcaaaa
    17641 gaaatacttt cttcgtttag ttccagtgga gaagatccat cagctgaagg aaacgatgta
    17701 gaagaggatg ctcgtccaaa ggtcatgtac accgatatca aaggaagtgt gaaggagcct
    17761 ggtatctatt ccttttcttc agaagaacgg gtctatgatg tactaaagcg tgcaggtgga
    17821 ttattagaag aagcggatag cgatcgaatc aacttttctg cgaagataga agatcagcaa
    17881 gtcctatata tccctgctgt aggtgaagag ctgccagaac atcttgatca gttggcatcc
    17941 tcggaaggaa aacagcctac agcagattcc gagcctccca aaataaatat caatacagcc
    18001 actccatcag aacttcagca gattcctggg atcggatcag tcaaagcgca agaaatcatt
    18061 cgatttagag aagaaaacgg atccttccag aaagtggaag acctgcagga gatttctggt
    18121 atcggtgaaa agacagttga aaaactaaaa aacttcgtta caattaaata gaatgattca
    18181 aaatcgactg gcacgagcaa taaatggacg ctaatggata ctaagaataa tccaattcta
    18241 agagcttggc aggacgagga ggtagtattt ttggatagga aatagcttga ttgttcgcaa
    18301 agaaacatta aaaaaaccaa tgctgaatgt ctatctccaa aataaaatat ctgggattca
    18361 tattatgaat acggctgtca gcggcaataa cagtcaagct ctacgagaac gctttgccaa
    18421 agatgtactt agctatacag cagataaagt atttattctc attgggacga atgatctagc
    18481 agagcataaa caactttcta aagaaacgta ccaaaaaatc tgcagtggat gatagttcag
    18541 ctgaaaaaag actatagttc tgatcatgct ttctttatca cgccaccagc agtcgatgag
    18601 acaaaacaaa aatatcggaa taacaagttg atcaaagaat atatctctgt tctcacagaa
    18661 gtagcaaaac gccatgattg tgaagtatcg atttttatac ggaatttctt gaacaaatgg
    18721 attcgaagaa tttttataat gggctaatgg atgacggact tcatttcggc agtgaagggt
    18781 aggatctttt gtcagacagg atgattcgtt atatttaaag aatattttgt ttaagcaatg
    18841 agtacttttt aatcaacttg aaaaaacgga ggaatataaa acatgttaga aaaatcttca
    18901 aaagcatgcg atacgaataa agaaaaagtt actgataagt cacataatga acgcattcct
    18961 tgggaccagt actttatggc tcaggcagta ctgttatctt tacgtagtac gtgtacacgt
    19021 ttagaagtag gagcgacact cgttcgagaa aaaaggatca ttgctggcgg ctataatgga
    19081 gcagtcagtg gagatgtcca ctgcatagat gaagggtgtt acgtcgtgga tggacactgc
    19141 ttgcgaacga tccatgcaga gatgaatgca ctcctacagt gtgcaaaact tggtatcccg
    19201 acggaaggtt cagaaatcta tgtcacacat tttccatgtt tggcctgtac aaaggctttg
    19261 ttgcaagctg gtgtgaaagg gatcaattat ctccatgact atcgaaatga tccttatgcc
    19321 caagcgttga tcgaacaatt aggtgtatct gtacaccaag ttgctttaga ctcacagcat
    19381 ttttcaaaac ttcaaaatga attaacagga aaacatgtct agcgttttag aaaggcatgt
    19441 tcgaaatcgg ttgatttttc cggcaattac gtcgatgatt atctgtttgt tgtattacac
    19501 tgacaattgg ggttatcgtt tgcttcttgc cggaattttt ttatggtttt cgctaaaaga
    19561 aggacgtttt atcgcgtact tgtgtatttt gtgtagcata acggtatcca tttcttatag
    19621 gctacaagag tattcatttt tagaggaaaa aacaacatct gcgcaaatta ctgtatttac
    19681 agatacgatt cgtgcaaatg gtaatttttt gacaatggaa ggaaggttat cagagaataa
    19741 acaaaagctc cagctttctt atcaagtaaa cacagaagca gaattgaaaa catggcttaa
    19801 cttccgagga aagggaatag aattgactgc tgaaggcgag ttcatctctc cagcaaaaaa
    19861 gcggaatctt tataattttg accaagaagc ttacttcagg aaccatagaa tttctggacg
    19921 gttccaaatt caaaagattg aacagatcac catgcaaaat cactggcgaa actggcttca
    19981 gagaaaaaga ggatctttta tctcctttgt ccaatctgaa tttccgcaaa aaacaagtgt
    20041 atatattaat tctttattat ggggctataa agatgcagga ttcagagaac cagaagaaat
    20101 ttttagggag agtggtttgc tgcatctgtt cagtctatcg ggtctacaca tccaatttta
    20161 tttagggtgg ctgtattatt tatttcgacg tatcggatgg ccactacacg tcagtattat
    20221 tccgttgagt ctactaacta tctgttatgt ttctattgca ggaagttcta tttctgtttt
    20281 gcgtgctagt ttgctgtttt tattgcgatt gctagtcaaa ttgctgaata agcattattc
    20341 caccatggac ttatttgcag ttgtcttttg gcttctttta ctatttgatc ctcgatttct
    20401 ttttcatgcg ggtggacagc ttagtttttt catgagtttt ttgtttatcc tgatttcttt
    20461 tgatggagat tggttgaaaa aaacggcctc tcacgtcctg tttactatga tcacgatgcc
    20521 gttgattgta tggcattttt atgagtggcc tattttaggt agtcttttta cattactgat
    20581 tgcaccggta ttcaagtttc tttttcttcc tggtgtgtta tttctcacac tcttcaatcg
    20641 ctggctgccg gaaactttac ttttgctgat agaagaggga ttaatcttat ttgagcgaat
    20701 aatcgatttt tcttcagggt cgtctctcat tataggacgt ttgccccttg tgctatctgg
    20761 gatgtatatt ttcggattac tgatttgtat ggataggttg aaagaacaac ctgtgaaaca
    20821 cttgttttat gttggtcttt tttcttttat ttttttgact ttgccatttt tacgcttttc
    20881 ttcaacgatc gcttttatcg atgtcggtca aggagacagt atctttttgc aaagtccttt
    20941 tcgaaaagaa acgattttga tcgatactgg aggtcagtta cactttgaaa gagaaaagtg
    21001 ggcgcaaggt atgatacgtc ctcctgctga aagaaatatc gttccatatc taaaaggtca
    21061 ggggataagt gtaatcgata aactcatctt gacacacgac gatacggatc atgtgggaga
    21121 attacccacg ttatctcagc attttactat caaaaaaatt tatatcggtt ggggcgctgg
    21181 acgaaatgag ccattacaaa ggcatttatc tgaagctcaa aaaaatggta ctgagatcat
    21241 tgaaatcaaa catggggaca ggatcagcgg ttattatgat ctttatgtat tgactccttt
    21301 tgaaaaggga gagggaagaa atgaagattc gattggtcta tggatggaat ataacaatag
    21361 gcgcttttta tttttaggag atttgaatca agcaatggaa aaacaattgc ttttgattta
    21421 tcctaatctg aaagccgatg tagtcaaatt aggtcatcac ggtagccgta cgtcaagtaa
    21481 cacagatttt ctttcccata ttgaagcaaa acatgggatt atatcctgcg gcgtaaataa
    21541 tcgttatggt cacccgcaca gcgaagtatt ggagacacta gaagaaaatc agataaaaac
    21601 atggcgaacg gatcaacaag gaatgatcac ctatcgctgg catccagttt ttcatcctca
    21661 tgggctagtg gaaacaatga tagactagta attctcatct gctcatggta ggataatttt
    21721 tgaaagtggt gagtaatttg agtatacaag cagagttaca aaaaatacgt aaagaaaaac
    21781 ttgcatcttg ttacttgatt ttaggaacag aaaagtttct ccaagatcaa gttcggacag
    21841 aaattgtcaa aaaaatccaa ttagagggcg aagatgactt aaatttttta agttttgaca
    21901 tggaaaatgc aagtttagat gaggtagtag ctgaagcaga gacgttgccg tttttcggag
    21961 atcaacggtt agtattcgta gagaatcctt attttttaac aggtgaaaaa gtaaacaatg
    22021 gaatcgaaca aaatactgat ttattgacgg cttatttgaa agaaccatta gaatcgactg
    22081 tattagtatt ctttgcgcca tatgaaaagt tggatgaacg gaaaaaagta acgaaacaat
    22141 tgaaaaaaac agcaattatc atcaatgtcc aacagttgaa tgaaaaagaa gtacgtcaat
    22201 atctgatgaa taccttagag aattcctcaa tcaaaatgga acgaagtgca attgatttat
    22261 tcttgaggtt gaccgactta gatttatcca aaatgatgag agagttacag aaattggtcc
    22321 tttatggaca aaatcaacag caaatcacgg taagagaagt agaagaatta gttcctaaaa
    22381 cgttagaaca taatattttt gacatgaccc aatatatttt aaaagggaag acggaacaag
    22441 ctttgcgttt atacgaagat ttagttatgc aaggggaaga aacaatcaaa attaatgcga
    22501 ttttgctttc tcaattacgt ttatttttac agacaaaatt tcttgtaaag attggttatc
    22561 agcaggcaaa tattgctgaa acattgaaaa tccatccata tcgtgtaaaa cttgccatgc
    22621 aggaagtaaa aaaattcgat gaacatttgc tggtacagtt gtttgatcaa ttagttgaga
    22681 tggattatca aatcaaaacg ggacaaatcg aaaaagaatt aagttttcaa ctattcgttt
    22741 tgcgcacagg gcagttgtta agaaatcgat aagaagaaag acgagggcaa agcatgcggg
    22801 aaggtcgttt gtttttgtcc tttttttgac aaataaaaaa atcggccgaa gccgattctt
    22861 ttatttagcg atttttttgc ttaaacgaga tttgtcgcgg ttagctttgt ttttgtggat
    22921 tagacctttt gattcagcca tatcaatagc tttaacagct tctttatata acgcatctac
    22981 gttgtcagca cctgaagcta cagcatcttc aaattttttg attgcagtac gcatagcgct
    23041 tgtttgagat gaatttttca cattagcgtt ttcgcttgta cgaacacgtt tgattgcaga
    23101 ttcaatgttt ggcattagtt tcacctccga taacttaagt tcttacgcgt gttaccgcat
    23161 aattcaacat tcattattat acctaaaggc agcgtgcttt gcaatatgaa catgacaagt
    23221 ttttttcctt aacagtcttt tattaggatt ttttttcagt aaaaacggat agtacaagct
    23281 gaaaaaaatc aaaaagacgc tattgtggga ggcaatagcg tcaaaccaaa ctagattttt
    23341 atttatctat ttaaacaaag tttgaatggg aatgtttggt actaagttat tatataacaa
    23401 agagaaccgt ttgcaaagtt ttttttgaaa agggatacga ttccctgttg ttctttgttt
    23461 ttctattaag aaactgcagt tattttataa ccagtatcct ttgatcagta aaggctattc
    23521 tggtgtaccg attattttaa tgtattcatc gatatttttt atcaacattt tttttatgac
    23581 caactggttc cgacggttcc atttgccact tacttccagc acatatttat ttaggggcaa
    23641 acgtaagata tattttgcca aatagcgatc tttaagcagg ccattgatca cacggtcctc
    23701 aagctgcaag ttgatcccag tcagcatcat tggctgttct tgtaaaacgt acgtatcaat
    23761 taaaagacct tcaaaagttt ccacaagcgt cttcctttct tttcaaagta acctgaaaac
    23821 aggcactaat tcttccattt tgatattcgt tgaagtggat gcgatcattc tttcttttgt
    23881 ccactgtaat ctagtataac aaaaaagagc agaagagcca aaaagaaaaa agagcatcga
    23941 attctatagg aagtctcatt ggcacggcct aaattattac aaaacattga ttaaaaaaag
    24001 cccctgcatc gcaggagcat cccctatatc gttttaatag gtcaatcaaa aagcagtatc
    24061 aaacacattt tgtatcaaat caggaaataa tagataagct aagcccagaa tcaaaagtac
    24121 cgctagaaga aaccagccga ttgttcctat gaaatacttt aatttttcga acattgtatc
    24181 acctcgttgt cagtatatca cggatcagta aattgggggt gttaagaatc tcctaaaata
    24241 gacaaaattt gttctatttt cctaaagcaa atacttgtaa gatacagtaa gtgataccag
    24301 aagcaatgac cggtccggcg gcgattcctc ttaagaagac aacacctaaa atcgtgccga
    24361 aaacaagtgc aacagtgatt tgtggatcag aagcaatcaa tcctacaccc ttggaagata
    24421 agacagccac tagcccaccg caaaaaacag ctacccagcc aataggggat ttaaaggctt
    24481 ctaatagatc acgtagaccg atttctcctg ttgcgatagg gattagaata gccgctgtga
    24541 tgatcgtcac cccaatattg attcctttgc cttgaagcca agggaaaaag gaacgggagg
    24601 ctggtatgag cttcataatc aaaacaaaag ctgccgctat catcagactt tgatttttag
    24661 ctaaaattgc tacaagtaaa ataattccta aaaaaagcca actttccatg aaatatattc
    24721 ctcactttct tcgaaacaat cataacatgt ttttagaaag ttacaaaagt aggaattcat
    24781 ccttcaagac ctattcataa gctgtttttt tcataaaata aacaaaatca taaaaaaaca
    24841 ctttgcttat ttgacagaag acagccgcgc tttgtatagt ggacaagact tacatttttt
    24901 tgaagaatca gaaagatcag gtaaaggtct tagtaaacgc aagccattca gaataaccaa
    24961 tattgtgctt ccttcgtgtc ctacaactcc aagaggtaga tttaaaaatt gcaggaagtt
    25021 agaagcaatc aacaagagaa taacaagaat cgaaaaaata atattttgtt tgatgatccg
    25081 ccgcattttt aaagatagcc gatgactgta gacaaattta tttaagtcgc ttttcattaa
    25141 gacgatgtct gcgatatcca tagcggcatc tgtcccttgc cccatagcga cacctaaaga
    25201 agcggctgct aaagcaggcg catcattgac tccatcacca accatcgcat ttacttggta
    25261 aagttcattc tcttttttga ctagttgtgt tttttcttcc ggagtacagc ttgcatagta
    25321 atgttcaatc cctacttgtt cagcaatggc ctttgctgtg ccagaatggt cgccggttat
    25381 catacttgta tggatgtttt gggatttaaa gtaggaaatt acttgttggg tatttgcttt
    25441 tggaatatct agcagaccta aaacagcaat taactgctga tctttagaaa gataaatcac
    25501 ggttttgcct tgattttcca aacgttcgat catttccgca atctctttgc tgattatcat
    25561 cgtctcagga tcatatgcgt gcttgccaac tttccattgc gcacctaaat aggtagtttg
    25621 taatccaaaa ccagtaattt cttctattgg taagtattgg atttcttcag gtattgtgca
    25681 atcgaaatat tgcaaaatcg catgagcaag tggatgcgtc gttttttgtt ccatagcaac
    25741 aagaagttgt tgtgccaaat ccttttcatc aaagaaaaag ctgtcagtta caactggttt
    25801 tcctttagtg agcgttcctg ttttatcaaa ggcaatcgct tttaattcag aaagcttttc
    25861 taaatggata ccgcctttga ttaaaatccc attttttgca gaatttgaaa gagcagctag
    25921 agaagctggt gtagcagatg cgacaagcgc acaaggagaa gccacaacta ataataccat
    25981 tcctcgataa aagctttcat cccaactcca acctaggaaa aagtggggga gaaggatcat
    26041 tagaggaaca gcaagcaaaa tcaattttac ataattattt tcgattcgtt cgataaatga
    26101 agctgtttga gttggcgtat tctgcgcttc ttctactaat tggataatct tagcaaagac
    26161 agtttcggaa ctcgtttttg ttactcgaat cgtgatggga tttccttgat tcagcgttcc
    26221 tgcatacatc tcatcgccta aatatttttc tacaggcaca gattcaccgc ttaatgcaga
    26281 ctcatcgatc gttgcctgtg tgctttctaa aaagccatct atcggaacag ctgctccttt
    26341 agcgacaaat accaagttat ccacctttag ttgagaaaca tctacttctt gcatttgtcc
    26401 attttctttt aataattggg ctttttgtgg ttgaagattc atcaaagcag taatttcttt
    26461 ttgactttta ttagttgtgt attcctctaa tgcaccgctt aaacaaaaga tgaatgtcaa
    26521 cattgctcct tcaaagtaat taccgatcaa acaggcacca atagcagcta aagccatcaa
    26581 taaatccaca ttcagtgtcc gatcattcca caattctttc aagccttccg atgtttgttt
    26641 atatccaccg atgataatgg cactgatata aaaaagtggg gtgatggtaa gagagaatcg
    26701 gtcgaagata aaaccgaaga tcataaataa aaggcagccg attgttgctg caaaagcttt
    26761 tttctgatta gtcatcgtta ttagacctcc ttgtcaaaac aaggatagca taataaaaat
    26821 gtaattgata ataataacag ctaaatgaga acgataatgg ttatcatttg gtgattaagc
    26881 ttgatgttgt ttttattatt tataatgatt attattaaag aaaataaaaa accagaacat
    26941 ttctcaaata agagaagtgt tctggttttt tctacattgc aataaaccaa taaccaatca
    27001 aaatgatacc aaggataata cgataccagc caaaagcggt aaagtcgttt ttcttcaaat
    27061 agcttagtaa gaatttgata gctaagacgg aaaccacaaa agatacgaat gttcctacaa
    27121 gaagaacaac agtttcttcc attttgaatg aattcccttc agctaaatat ttgacgatct
    27181 tcaagccgct tgcgccaaac atgacaggaa tccctaagaa aaatgaaaac tctgtagcga
    27241 caaaacgcga agcaccaatc aagatagatc ctaggatcgt tgcgccagaa cgggatgttc
    27301 cgggaacaag tgccaagact tggaataacc caacaagaag agctgcttgg tacgtaaagt
    27361 cattcagaga agtccaacga ggtgttttgt ttttgttgcg tttttcgaca ataataaagg
    27421 caataccgta aacgatcaaa acaatggcaa ctggtaagaa cttgtggaaa tttgcttcta
    27481 accaatcatc caaaggcaaa ccaatgatga tggctggtat gcaagcaacg atcactttaa
    27541 accaaagtgt ccacgtatct tttttttcta ccgttgattt acttggagaa aatgggttta
    27601 acttgtgaaa ataaagaacg acgacagcca agatcgcccc taattggata acgacattga
    27661 acatcgacat aaattgtgaa ctggcatcca gtttgatgaa ttcatctgct aggatcaaat
    27721 gaccagtact actgatgggc agccattccg ttattccttc aataataccg agaatcactg
    27781 ctttgataat atttgcaaaa aacataaatt ccttccttcc caattaagat acatcttata
    27841 gtataccgtt attttttgag aaacccaatc tgttttttaa tattaagaaa aacttgagaa
    27901 ataaagcaga taagtacacg aagagaaaaa agaagacaaa gccctcgttc agactttgcc
    27961 ttttatgatt ctctggtaaa aattaaaata tccccattat agaaacgagt cgcgactatg
    28021 tgttttcccg ttatcgatat aagacgtatc attgatgccg tggatagaaa atcgaccgtt
    28081 tgcataagtg acctttgtta cgctgccatt taaaagccca gcacgaacag gctgtttagg
    28141 atcgatcaga ttaagcaaaa aggcgatcgt caaaccatga gatacgacta aaacatttcc
    28201 accaccattt ttttcgcagt gatgggcgat atcttcaaat cctgtccata cacggtctcg
    28261 caatgtttca taagtttccg cccacccagc ggtatctaag ttgtatacag cattagcaat
    28321 ttgttcaaaa gaaacttctg tggtaaacat ttcatcatat gttttaaaat tcaatacacg
    28381 agggatcaca ccccacattt ccatgtcata tccaccttca agagacccga aacaccattc
    28441 tctaatcctt ttttcaataa aataaggaat ttcttttccc ttagaatgtt ctcctagaat
    28501 aatacgggca gtttcgattg cacgaccact gtcactagaa taagcaaatg aaaagtcgac
    28561 attttttaac ccgcgaccta aataatgaat gccttcttgt ccttgctttg tgaggggtgt
    28621 atcacaccag ccttgtactc gttcaattgt attgaacatc gtttttccgt gtcgtacgat
    28681 ataaagtgta gtttctgtca ttttattctc ctcctaagtc gttttaacga ttgaaaaaag
    28741 gatttgtcct tttttcctgt tcaatcgtcg ttgcatcgcc atgtcctgga tagactgcaa
    28801 attcatcagg taatacaaac agctgggtcc gaatactatg aagtaattgt tctaaattcc
    28861 ctgtatacag atcggttcta ccgatacttc ctctaaacag ggcgtcgcca gaaacgacaa
    28921 aatcatcaaa aataaagctg acgcttccta tagaatgccc tggggtaggg acgacgcgaa
    28981 aagacatgtt tcctaaagta taagttttca tttcaaattc atattctgca ggcgagacaa
    29041 taacatcagc aatatcatca tgtctaccga gtccagaaag attcaaaatc ggatcaccta
    29101 gccatttttg ctctagtggg ctgacataaa ctgggatttg ataatacgcg cgtaaaggtt
    29161 ccactgcacc gatatgatcg tagtgagtat gggtaagcaa aatcgctacc ggctgttggt
    29221 ttgttttttt gatttcttgt ttgattcgtt ccgcatcttc tccagggtca atgatcaaaa
    29281 gtgcttcttc gttgtagacc aaataacagt tttcttcaat cattcctgtt tttaatcttt
    29341 cgatatgcat gtgcattcct ccttgatagc caaaacttct ttcatccatt agtataacgc
    29401 attcattgct aggaaacaaa aacaggtact tatttctctt tacttatctg aattatcaga
    29461 ctatatgaaa agtatttgga tattctaaag agaaggaaag taagttttca tagcatttta
    29521 atatgtcctt gttacattag attcatccaa aaatccaaat tctatatcct atatccctgc
    29581 ctaatcagca gggtgttttt ttatgtttgg aaagagagct ctttttgcta aactaggttt
    29641 tttcaggtaa tcttagagag aaagaggtgg tggaaatgat caaacagtca aaaatatctg
    29701 tgcggcactt agtagaattt attttgcgac gtggaagtat cgacaatcgt aaaaaaagta
    29761 accatactgc attagaagga gcgagaatcc accgcaagct ccaaaaagat gcgggagaaa
    29821 actatgaaaa agaagtcttt ttaaaaacaa cagttcaatt agatgaatat tacctgatcg
    29881 ttgaaggccg tgcagacggt attttcaaaa gagacggtat ttattatata gatgaaatca
    29941 aaacctccga accacgattt gaagatttag aaccagaaca agtggaactt ttctttcatc
    30001 aagcaagggt gtatgcctat atctatagtc aagaacatga tttacaagaa ataaatctgc
    30061 aattgactta cttccagacg gcagaagaag tgatcacgag aaaggttgaa catcagacaa
    30121 gagagcagtt agaaactttt tttaaaaagc tgactgaaga ttataaagaa tggcttgttt
    30181 ttcaagaaaa ctggcgcact gtacgcaatg cctctttgat ggctttgaaa ttcccccatg
    30241 atgagtatag gaaaggtcag cgtgaactag cggtagctgc ttataaaacg atccgtacga
    30301 aacagaaatt gtttgtagaa gcacctaccg gaacaggaaa aacaatctct acgttattcc
    30361 ccgctttgaa agctgtaggg gaagaagagg gagaaaagat cttttatcta acggctaaaa
    30421 caataactag acaagtagct gaagatgcta tgacagcatt aaaagataca ggagctgaag
    30481 taaaaagtgt cactttgacg gcgaaagaca agatctgctt tttagatgag acgacctgta
    30541 atccagatca gtgcccatat gccaatggtt attacaatcg gattaatgaa ggtttatggg
    30601 atcttttgaa tcatgagaat cagatcactc gtgaagtcat cgaaacttat gcaagaaagc
    30661 atacactttg tcctttcgaa ctatcattag atgtcagtat ctggtgtgac gtaatcattg
    30721 gggattacaa ttatttattt gatccaactg tttatttaag acgttttttt gaagatgaaa
    30781 aaaacgagga ctatctgttt ttgattgacg aagcacataa tttggtgaat cgttcccgag
    30841 agatgtattc ggcagaacta tcttatgaaa aaacgaaacg aagtaaggaa gctataccaa
    30901 aggaattcaa aaaacttcat cgccgtttca acaaactatt aaaagaattt gattcgatcc
    30961 gagaaatagc aaaagaagat cattgggatt atcatcatca aaaagcaccg gctgaatctt
    31021 tagtaaaggc aggttatcaa ttaagcgaaa aaattaaaga atggctggca gaatttcctg
    31081 agcacccatc acaagaacaa ttgcttcctt attattttga tttgcttcat tttttgaagg
    31141 tcagtgaatt ttatgatgat cactacgaaa caacagtcga aaaaacgtat cgcgatctga
    31201 tagtaaaaga gttttgtatt gatccaagct tgtttcttga gcagtcgttg gataaaggaa
    31261 aaagcagtat tcttttttct gctagttttt caccactctc ttattatcag gaaacactgg
    31321 ggggacaaaa aagtctggct tacaaactgc cgagtccttt cccagaagga aaccagcaag
    31381 ttttcatcgc aaattaccta gagacgactt atcgaaaaag ggaacacagt ctggcaaaat
    31441 tagtagagac gattcatgct tttgctgatg gaaaggtcgg gaattatttc gttttctttc
    31501 cttcttatca atatttagat cttgcggtgg aagcattcag acagcaatat cccgacagca
    31561 atgtgctaat ccaagaaacg gacatgaatg aggaagaacg cgaacgattt ttaacaaaat
    31621 ttcaaacaaa tcccgctgaa acattgattg gattttgcgt acttggcggg attttttcag
    31681 aaggaatcga tttgagaggg gaccgattga tcggcagtat gatcgtaggt gtaggtttgc
    31741 cgcagatgaa tcatgagcag gaactgatca aatcatatta tgatgagaag gaacaccttg
    31801 gatttgctta cgcttatcag cttcctggaa tgaacaaagt cttgcaagct ggtggtcgag
    31861 tgattcgtgg catggcggat caaggaatcg ttgtattggc agatcaacga ttttctagtt
    31921 tttcttatcg acgtttattc ccacaacatt ggcaaacagc aagagaagta cgaagtgttg
    31981 gggaattgga agaaggaata cagcgttttt ggttatcaaa agaagcagta aatcaaaaaa
    32041 acgaggaatg acagacgtca gcctcgtttt ttgattaacg atagttagtg aattgcaact
    32101 cgattggtaa gtcaagttta cgtaataaag caatcacagc ttgtagatca tcacggttct
    32161 tacctgtcac tctaatctga tcttcttgga tttgtgcttt tactttcagt ttttcatttt
    32221 tgatagctgt agtgattttc ttcgcgtttt cacgatcgat tccgttgacc aaatcggcag
    32281 tttgtcgagc tttgccacct agagcgtgtt ctgattcgga aaaatggata tttttgattg
    32341 gcacatttct tttgatcagc ttaccaaata atacatcttt gatctgttcg attttgaaat
    32401 catcatcgct caatacaact agattgctgt tctctaattt gatttctact gtagaatcct
    32461 tgaagtcaaa gcgattggac aattcttttg ttgtttgctg aatcgcattt tttacttctt
    32521 ctgtattgac ttctgataca atgtcgaagc tagcatcttt tgccatagga accctcctga
    32581 tatacatata aatatttacc atacatctat cttatcatag gattagggca gattcttacc
    32641 tgtttgctaa aattcttctc atttgagttc gaagtatttt attttaatag ataaactgtt
    32701 attcttacat ttggaatacc aaagtagggg agaggtgctt gtgagtaaaa ttatgactgc
    32761 gtatcataaa aatgaaagat taaagaaaat agtggttatt ttgataaccg gattgaccgc
    32821 tgcggtcgct ttaaattatt ttttgattcc agccaatgtc ttttctgcag ggatgaatgg
    32881 gatcgctcaa atcatttcct cgcttttata tgaatggttt catatcaaga tagacacagg
    32941 aacatttatt tttcttttga atatccctgt gtttgtatta gggtttctca aagtaggaaa
    33001 acgagcaacg atccttagtt ttgtgaatgt tgtatgtgta tcggtgatga cgatgctgct
    33061 tccagtcgga cagatcactg ataatatttt gatgaatgcc ctagtaggcg gtgttttatt
    33121 aggtcttggc gtaggattat ctttaaaaat gggctttaca actggtggga tggatatcat
    33181 ctctcttgta ctttcacaaa caactggaaa aacagtcgga aactacatgc tgattctaaa
    33241 tggtatcatt gtagtcatcg caggcttctt ctttaactgg gaaagtgcct tgtatacgat
    33301 tatttccatt tatgcaatga ctcaagtaat tgatgccatc cacacgagtc atcaaaaagt
    33361 aacagcaatg atcgttaccg tcaagccaga agaggtaaca gaagaactag caaaacggat
    33421 ggtacgtgga atgacattac tgccgtctgt cggtggctat tcaaaaaaac aagggaacat
    33481 gatcatgatg gtcatcaccc gctacgaaat gtatgatctt gatcagatcg ttcatgaaat
    33541 cgacgacgat gcatttatca atattttacc aactcattca gtatttggcc ggtttgcaaa
    33601 tgaaaacgaa caaaaaatgt atcgaacaac tggcgtattg ccagaaataa aaaatcgttc
    33661 acaaaaaaaa tgaggaatca ggtttatttt agaataaatt taatctatta catctaataa
    33721 gaacattttt ataaatttaa acgagaatct tacttctaaa ttctcattta caagcagcta
    33781 aaaaaagagt atgataaata aaaatcgtgt taaggagtgt cgtacgatga gcgaagcaaa
    33841 ccaagaacgg tctggacaag aaatagaact aatcaaagaa cgtattcttg ctgccttgga
    33901 aatggtgatt gatcctgaat taggaatcga catcgtcaat ttaggcttga tttatgatat
    33961 agaattcaat cctgaaaatg gagaaacagt cattaagatg actctaacaa ctatgggctg
    34021 tccattagca gatattttaa cagaatctat ccatgacgca ttaaaagaag tgccagaagt
    34081 aacgaaagca gaagtcaaac tagtctggta tccagcatgg acgacagata agatgagccg
    34141 ttatgcacgg attgcgttgg ggatacgata gaaatcttgg ctgactagct atgagaagca
    34201 aataggaaaa acaaaaaagc cttttatcct ttctagaaga aaagagataa aaggtttctt
    34261 tgtttataaa attttgtctg ctatattata agttaattaa ataaatgtgt tctattgata
    34321 aaggacattc aaatcccttt ccttttggaa tccttctttt ttacatcagt ttgattttcg
    34381 tatagtattc tttctcatct tcacgataaa tcgcagcccc atttttttgt tgtgctttaa
    34441 gactgccaag atttttttta tttacagaca aaagtgcctc atcaatgccg attttttttg
    34501 cctcttgtag tactaatgct aatccttgtg tggcatatcc ttttttacga taattagggg
    34561 agatgccgta accaatatgg ccagcgccgt ttcttaatgt ttcgttcagt ttatggcgca
    34621 aattaaaaat tcctacatac tcatgttcat cactttctaa aataaagacg gttgcgggaa
    34681 cgtaactctc aggaagatcg attcctaaag acgttttttc gcattttttt acataatcta
    34741 aaaattcagc aaatgtatag ccataagcat tgttgataaa tccactctca tttggtccaa
    34801 agctagtttg tagctgataa gctttcttta aatctgcttc ccatagtttg atcagtttca
    34861 tcgtattcac ctctttaagt aaccttattc atttgttttc tactatagca ctattgttgt
    34921 aaatagccat gcctattttt atttattgtg tcatatcgta ttaagaatca tcgaacaata
    34981 caaatatgaa ttacaataaa aaaattattt gaagttcgtt cattcgttac ttatgatctc
    35041 tagaagaaag aaaacgtgaa ttacttcagc gtctagaatt ccagaaggat agtaatcaac
    35101 agaagaaata atcaatatcc aaatgaaaga agatagctga atcgagaaaa agaggaaaaa
    35161 agaaatgaag aaaaaaataa aatagttact aatcccagag tcgttcgttt aacattgaaa
    35221 tcgatcatat ggataaaaca gattttttca ccaaccctca atagatgatg gagttgctcg
    35281 gatgaattat ttgtgtagat ggcataaggg agggcgtaca gaaatgaaaa agaacaaaaa
    35341 tatgttcttg acaaaaaaca catgtaatcc taaactaaaa gatgtgatgc ggctatggcg
    35401 gaattggcag acgcgctagc ttcaggcgct agtgagggtg acctcgtgga agttcgagtc
    35461 ttcttagccg cataatttgt ttttttataa agagaaggta cacctggagc gatgagaaag
    35521 aaaataacat attatctaaa agaatatagg aaaaatttta aacaacggaa ccagattcaa
    35581 ataaatcaaa aaaaatttgt gtgaaggtgg gacagtttaa aacctaatag attgattcaa
    35641 atagtagatt tatagattct gatacaagaa atactggcaa acgatagtta aggaataaag
    35701 actaaactta aactcaaaaa gaggtcatgc gaaagaataa atcgcatgac ctcttactat
    35761 attttgaatg tgtttaaacg ttcaaataat gaatcttagt accaaccgtt tgcttcccag
    35821 aatgctttcg ccgcttccca agaaccataa cgtgaagtaa catattgttc tgctactctt
    35881 tcttggtttg cagcagagta gtcaccattt aagtaagaag aatctaattg gtaacggccg
    35941 atgtaacgtc cgttcgttgc tgtgtaagaa ccgcttgatt ctttttgtgc gatccattct
    36001 tttgcagagc ttgtacttgc tggagtagca gcagtttctg ttacagcagg agcaggttgt
    36061 gtttcagcaa caggcgctgc ttgtgtttcc gtttgaaccg tttcttgtac aacaggttgt
    36121 tcagcttgaa caggttcagc ttggactggt gcttcttgaa cggctgcttg aggttcagca
    36181 gctgcttgtg cagagccttc tgttgggatt gttaattgtt gtcctgagta
//
LOCUS       DACDYI010000022        34662 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.022, whole genome shotgun sequence.
ACCESSION   DACDYI010000022 DACDYI010000000
VERSION     DACDYI010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.022"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            172..444
                     /locus_tag="IWM52_001056"
     CDS             172..444
                     /locus_tag="IWM52_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973323.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
     gene            459..1382
                     /locus_tag="IWM52_001057"
     CDS             459..1382
                     /locus_tag="IWM52_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9973324.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(1522..2274)
                     /locus_tag="IWM52_001058"
     CDS             complement(1522..2274)
                     /locus_tag="IWM52_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9973325.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            2497..2859
                     /locus_tag="IWM52_001059"
     CDS             2497..2859
                     /locus_tag="IWM52_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP9973326.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            3496..4968
                     /locus_tag="IWM52_001060"
     CDS             3496..4968
                     /locus_tag="IWM52_001060"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAP9973327.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            4980..5804
                     /gene="nadE"
                     /locus_tag="IWM52_001061"
     CDS             4980..5804
                     /gene="nadE"
                     /locus_tag="IWM52_001061"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAP9973328.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(5877..6767)
                     /locus_tag="IWM52_001062"
     CDS             complement(5877..6767)
                     /locus_tag="IWM52_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAP9973329.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            6965..8482
                     /locus_tag="IWM52_001063"
     CDS             6965..8482
                     /locus_tag="IWM52_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP9973330.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            8472..9743
                     /locus_tag="IWM52_001064"
     CDS             8472..9743
                     /locus_tag="IWM52_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9973331.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            9918..10814
                     /locus_tag="IWM52_001065"
     CDS             9918..10814
                     /locus_tag="IWM52_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9973332.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            10828..11691
                     /locus_tag="IWM52_001066"
     CDS             10828..11691
                     /locus_tag="IWM52_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9973333.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            11697..12260
                     /locus_tag="IWM52_001067"
     CDS             11697..12260
                     /locus_tag="IWM52_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP9973334.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            12270..13794
                     /locus_tag="IWM52_001068"
                     /pseudo
     CDS             12270..13794
                     /locus_tag="IWM52_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
     gene            13806..14765
                     /locus_tag="IWM52_001069"
     CDS             13806..14765
                     /locus_tag="IWM52_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAP9973335.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            14851..15930
                     /locus_tag="IWM52_001070"
     CDS             14851..15930
                     /locus_tag="IWM52_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9973336.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(16182..17582)
                     /locus_tag="IWM52_001071"
     CDS             complement(16182..17582)
                     /locus_tag="IWM52_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAP9973337.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            17800..19461
                     /locus_tag="IWM52_001072"
     CDS             17800..19461
                     /locus_tag="IWM52_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAP9973338.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            19418..20116
                     /locus_tag="IWM52_001073"
     CDS             19418..20116
                     /locus_tag="IWM52_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP9973339.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            20132..21556
                     /gene="araA"
                     /locus_tag="IWM52_001074"
     CDS             20132..21556
                     /gene="araA"
                     /locus_tag="IWM52_001074"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAP9973340.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            21863..23023
                     /locus_tag="IWM52_001075"
     CDS             21863..23023
                     /locus_tag="IWM52_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAP9973341.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            23141..25000
                     /locus_tag="IWM52_001076"
     CDS             23141..25000
                     /locus_tag="IWM52_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP9973342.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDHSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            25110..25544
                     /locus_tag="IWM52_001077"
     CDS             25110..25544
                     /locus_tag="IWM52_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973343.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(25983..27005)
                     /locus_tag="IWM52_001078"
     CDS             complement(25983..27005)
                     /locus_tag="IWM52_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAP9973344.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            27133..27486
                     /locus_tag="IWM52_001079"
     CDS             27133..27486
                     /locus_tag="IWM52_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP9973345.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            27498..28664
                     /locus_tag="IWM52_001080"
     CDS             27498..28664
                     /locus_tag="IWM52_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9973346.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(28756..29376)
                     /locus_tag="IWM52_001081"
     CDS             complement(28756..29376)
                     /locus_tag="IWM52_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAP9973347.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(29568..29864)
                     /locus_tag="IWM52_001082"
     CDS             complement(29568..29864)
                     /locus_tag="IWM52_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP9973348.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            30068..30718
                     /locus_tag="IWM52_001083"
     CDS             30068..30718
                     /locus_tag="IWM52_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9973349.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            30734..32461
                     /locus_tag="IWM52_001084"
     CDS             30734..32461
                     /locus_tag="IWM52_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9973350.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            32458..34350
                     /locus_tag="IWM52_001085"
     CDS             32458..34350
                     /locus_tag="IWM52_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9973351.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
BASE COUNT        11241 a         5978 c         7213 g        10230 t
ORIGIN      
        1 attcactaat aataaagata tatttacagt atcattataa tgtattcaat ttaaaataag
       61 agactttggt aataatgaaa gaggaataaa acttttcttt ggcaaaacaa actccttgct
      121 atgagacgcg tttcatacac tatactcaaa gtatcaaaag gagggcttgc aatgaatact
      181 tatgtagaga taacagatgg cacaacgaac aattattttt atgcgtttgt cgagttcaag
      241 ggatccgtat taacgttgta cagttttcaa gggttgaata ggaatatagt aaaagaaata
      301 ccaatgaatg aaatcgaaaa tttgacaaaa gatatatatt ggggcgggca gcggatcagt
      361 ttttcgcatg aaggaaaaat gtatcagttt tttgaatgcg gtccagctgt ggtagactat
      421 ttacaagaaa atctttttgt ataggtggcg aagcagcagt ggcaaacatt cgagatatcg
      481 caaagctcac aggttattca gtttctacta tttcacgtgt aatcaatgga catccatatg
      541 tagatgagga aaaaaggaaa gaaatacttg cggtcatgaa agaagtcgat tatatcccta
      601 atgcaaatgc cagacagtta agttatggaa aaacgaaaag cattggagtg attctgcctt
      661 ataccaatca tccgtatttt gatcagttga tcagtgggat cattgaagca gcgtttgatt
      721 atgattacaa agtgacactt ttaccgacgg gctatcataa agaaaaagag cgaaggtatc
      781 ttgaagaatt tgcagctaaa gctttcgatg gattgatcat tacgtctcgt gccaatacac
      841 tagatgattt attggcatat caaaaatatg gacctctagt tttttgtgaa gaaatcaagg
      901 gaaaacaggc aagctgtgtc tttattgatc gagaacaatc aattacagaa ggtcttactt
      961 atttgaagca gcaaggtata aagcaattgg gcatcacgtt ggggcgaagt ggcaggctaa
     1021 gttataactc aaaaatcact ttgcagctat gtcatcaact ttttccatct tttgatgaat
     1081 cacttgtttt ttgggattgt atagatgcag agaaaggaaa acaagcaggc ttctttttta
     1141 aagagcttgg agttgatggt gtctttacaa attcggatat ggttgcagca ggcatcttgc
     1201 aaagctatct gcaagataag acaccagtgg tgattggacg cgataatttg ctgatcagcg
     1261 agcttcttgg tttcccaacc atcgaccacc atctggaagc ttgcggagaa atggcatttc
     1321 aattgtttct tacagaaaaa aaggataaag tgaaaatacc ttatacgttt attcaacgat
     1381 gaatatattt aaagtaggat gaaaaaactt gtgaaagaat ggagcagaca ccaaaagtta
     1441 gcataaaaat ctaacttggg tgtctgctgt tttgttatta ggtcatttta cgtgtacttt
     1501 tttgttggat catgtaaaag attacgcctt tttttggata atttcattta aagcttttgc
     1561 tgcaccatga ccacttcgct cgtcataata tgcagtgaac cgttcatcgg cttcgtacat
     1621 cattcctagt cctttatgcg cttttggctg atattcaggc cagctgtaag agaaccactt
     1681 cttatgagct tggaagatac ggtctgctaa gctttcatct tctggttctt ttaagtaaag
     1741 agtgagatca aataacatct gcttttcaac agtttgcatt tcttcatacg tttctttatc
     1801 catattcatc catttttcat tggatgctgt aacctttttt tttccgtatt tttgtcgaat
     1861 ctcttgtcca taggattctt cattttcttt caacttttgt tgtttaaacc cttcaaattt
     1921 ttctttgtca ctcatttttc ttcctccttc gtacatatca agtgtctgtt ggacagttgt
     1981 caagagggta tcgatttgtg cccgtttttc cagcaacatt tgctgatgct tgattaaagc
     2041 tttctggtgg tcaaaagatg gattgtctaa gacctcttgg atatcatcca acttgaatgc
     2101 cattgttcga taaaataaga tgtgttgcag acgatcgact tcttttttgc cataaatccg
     2161 ataacctgaa gaattgatcc tagctggttt tagcagtcca atctcatcat aaaagcgcaa
     2221 ggttctcgga ctgactcctg atatctctga cattttcttt attgtgtact ccattatttt
     2281 tcctcctgac aaatgtaagc ataatccttt acgtaacgag atggtcaaga aaaaattgta
     2341 tttttttctc acgaaattat gttatactat taagtgataa aaggtaaatc cttttgtctt
     2401 taaaggttca attgtaaccg attccttaat gctaggaaag atggtaacat aacttaagat
     2461 tggatctaaa tcgactatat agaaaagagg aatgagatga agctaatcga aacacctgtg
     2521 aatagtaatt taaatattaa gacattttat cctaaagtag tggaattctt ttttgggaat
     2581 acggctatta attattacaa attattttcg ttagatcgaa cacaactgct attggtagac
     2641 acatatgata aaaagcaagt cgtgatgatc aatacgaaga aaaaaatcac aaggcaagaa
     2701 atcgactatg cgattcatca cgttttaaaa atgacacgag aagatgtcaa agtccatgta
     2761 ggagtgaaac aggagctaga acgtgccgga atacaattca aacgtccaaa taaagatatt
     2821 gtagtgatag aacaaaaaaa tacgaaggat tctatttaat aacgactaat aatcatcttt
     2881 atggtaacct gctccatatc taatgtatac aataaaaaac aaggcgagac tgattgatgt
     2941 ctcgccattt ttgtttccat tctattgaca gctgctttct tactttgtat ctttttaaaa
     3001 acgtgtgata ttttttccgc gagttttttc aacgcaaggt atatcttgtc ataattcgcc
     3061 tttccaaaaa aattcaatac ataataagtt atatccgttt ctttggggat aagaagcaaa
     3121 aaaggaaaga cgtattattg cttcaaaaac ttttgccatt gattatgttg tagagacatt
     3181 tggttatatt tgttcaatca tcggtatctt aacggctacg attgtagtag gaaaatctac
     3241 ttattttatc aagtaagaaa aatagaaaac gctaatttct tcgtgtctcg gatacattga
     3301 ataaaataag tattctttct cgtttaggtt aaataccaat aagaaaggat agttttagca
     3361 gatggagttc atttttagca agaaatatcc gtaaaacatg tgctttttca gaaaaaatgg
     3421 aagatttctt ttcctattaa ttttttttgt tataatgggt aggaaatgaa aacttgccaa
     3481 gaggagtaat acaagatgaa taatacttac ccagacgaca gtttgacgct gcatacggat
     3541 ctatatcaaa tcaatatgat gcagacatac tgggaattag gtcgagctga tttacatgct
     3601 gtttttgaat gttactttcg agaaatgcct tttaaccacg gatacgcggt ttttgcagga
     3661 ttggaacggt tagtcaatta tttagagaat ctgacgttca atgattccga tattgcttac
     3721 ttgcgtggat tagaagtcta tcctgaaggt tttttggagt atttacaaaa ttttgaattt
     3781 aaagcaacag ttcgttctgc tcgtgaagga gaactagttt ttgcaaatga accattaatc
     3841 caagtggaag ggcctttagc gcactgccaa ttagtagaaa cagcactgtt gaatatggtc
     3901 aatttccaaa cgttgatcgc gacaaaagca gctcgtatca aatctgtcat cggagaagat
     3961 ccactattgg aatttggaac tagacgggca caagaactgg atgcagcagt ctggggaact
     4021 agagcggcat atatcggcgg agcagatgca acaagcaatg tacgtgctgg gaagatattt
     4081 gggataccag ctagcgggac ccatgctcat tcgttagtcc aatcttatgg aaatgattat
     4141 gaagcattta tggcctacgc taaaacccat aaagactgcg tgttcttagt cgacacttat
     4201 gatacgttga aatcaggtgt gccaagtgcg attcgcgtgg cgaaagaatt aggtgacaaa
     4261 atcaacttcc aaggcgtacg catcgacagt ggagatatgg cctatatatc taagcgtgtt
     4321 cgggaacaat tagatgcagc cggatttacg gaagcaaaaa tctatgcttc gaatgacttg
     4381 gatgaagcaa cgattttgaa tttaaagatg caaaaagcta aaattgatgt atggggtgtt
     4441 gggacaaaat tgatcacagc ctatgatcag ccagcccttg gtgcagtatt caagctagta
     4501 tcgatcgaag atgatgaagg aaaaatgatg gatacgatca agttatcaag caatgcagaa
     4561 aaagtaacta ctccagggaa aaaacaagtg tggcgcatca ctcgaaattt tgatgggaaa
     4621 tctgaaggag actacgtgac attatgggat gaagaccctc gagaagaaga agctatcttc
     4681 atgttccatc cagtgcatac gtttatcaat aaaactgttc gagactttac agctcgccca
     4741 gttttacaag atatctttat cgaaggaaaa agagtttacg aattacctgc actaaacgaa
     4801 atcaaagaat atacaaaaga aaatctagat tcactatggg aagagtataa acgagacttg
     4861 aatccgcaaa aatacccagt cgatctgtct actgaatgct ggaaccataa aatggctatc
     4921 atggaacgaa tgaaaaaaag cgtagcagaa cttcatacag aagcatagga ggagaataaa
     4981 tgagcctgca gacagaaatt atcaatgaat taggtgtcaa accaacaatt gatccaaaag
     5041 aagaaatacg taaaagtatt gactttatga aagcatattt gaaaaaatat ccgtttctta
     5101 aaacttttgt actaggtatc agcggcggcc aagattctac tttagcaggc cgtttggcgc
     5161 aactaacgat ggaagaaatg cgtgaagaaa caaaagatga aaactatcaa tttatcgctg
     5221 ttcgtcttcc ttatggtgaa caagcagatg aagaagatgc aaaagctgct cttgacttta
     5281 tccagcccga tgttagtctg cgagtaaata tcaaaccagc tgttgatgca caagtacaag
     5341 tattaagcga agcaggtgtg ggaatcagcg attttaacaa aggaaatatc aaagcacgtc
     5401 aacgtatgat tacgcaatat gccgtagctg gagaaagagc aggagccgta ttagggacag
     5461 atcatgctgc agaaaacatt actggattct tcacgaaatt tggtgatggt ggagccgaca
     5521 ttttgccatt attccgtttg gacaaacgac aagggaaaca attgcttcaa gcattgaatg
     5581 caccggaaaa attatacaca aaaattccga ctgcagatct tgaagatggg aaaccaatga
     5641 ttgcagatga agtcgcacta ggtgtcacat ataatgaaat tgatgattat ctagaaggaa
     5701 aagaagttcc cgctcaagca caagaaaaaa tcgaagcatg gtggaacaag acacaacata
     5761 aacgacattt gccaatttcc gttttggatg atttctggaa ataacaataa agcaaaaaca
     5821 gccgtccctt catagggatt ggctgttttt gttttataaa atagaaaaga aaaatatcag
     5881 ctgtagtaaa cttttaattt gatatcctga tcgtaattac cgaatccttt accaaataaa
     5941 gtacagcccc ccacatgttt ggcatcgtct ttcacttcga ttcgcaaatg ccaagtgtct
     6001 tgctctttag ggatgtcgtt gatcgtgatg tttgacaagg gttctccatt catattcgtc
     6061 aagtgtttgg taattcggat cgtcttcagc aagccgtatt gattaagatt atctggatac
     6121 caatcaggcg tatatttacc gcggatatca gcaaaatctc cagggctcgt ccatgttcct
     6181 agctccacac catttaggct gaaagtgata tctgaaggcc aattgtcatt tgaaaaagga
     6241 aattcagaag aaatttccac agaaacttcc aacatctcca atgtatcctc tgtatttaaa
     6301 aaattaggtg cctgatactc gacaaatccg ctggtaaacc agagaatgcg ggcatccatc
     6361 cttcgaggat ccataaaata gcgtggttca tccactttcc cgataaaatc atggatcgta
     6421 gctaaaccgc atgttggttc aattgcataa tttgtatagt gaccaatagg tatcgatgtt
     6481 tctttcgtat caaaagcatt gaatattttt tctggaaaac tgatatcgat tttatcgaca
     6541 cgaaggctcg aaattttttg ttgtcctact ttttctgttt tgatgatgtt tgcttcctct
     6601 agctttttca cgtgcatcgt cgtgatagca ctgctcactc caagttcttt tgccaaatct
     6661 ttgacattca tctttttttt ggaaagtagc tggatgatct tgatccttgt tttactagct
     6721 aatgcttcat ataccggtaa ggattcttcg tttatttgta gttgcatata tatcacctct
     6781 taaaaggttt ttcttatata ttaacatatt tgttaataaa agttatatta aattatttgt
     6841 tttaatcatt attaatattt tggttaatta aaatcgataa aaaataagta agtttaacat
     6901 ttatattatt gacaacgctt tcaacgaggt gtattattca tgtataaatt aaaggaggtc
     6961 agttatggaa gccacaatgt tgattagtag tgtcaagcgt gtaggcaaag ttgatccgcg
     7021 gttgtacagt tcatttattg aacatatggg acgagcagta tatgaaggga tttatcagcc
     7081 agatcatcca tcagctaata aaagtggatt tcgccaagac gtcattcact tggtaaaaga
     7141 attgaacatt cccatgatta gatatcctgg tggaaatttt gtgtcaggat ttcgttggga
     7201 agacagtatt gggcccatag caaatagacc tagaaggttg gatttagctt ggcggacaat
     7261 tgaaacgaat gaagttggga ttcatgaatt ttataactgg tgtcagcatg tgggggccga
     7321 aatcaatatg gctgtcaatc tgggtactcg tggagtagat gcagctcgta atttagtcga
     7381 atattgtaat tttcctaaag gaacttattg gagtgattta aggcgttcga atggtcaaga
     7441 gagccctttt ggaattaaga catggtgttt agggaatgaa atggacggtc cttggcagat
     7501 tggacataaa acagcggaag aatatggacg gttagcagag gaaacggcta aggcgatgaa
     7561 actcgtagat gattcaattg aggtggtttt atgcggaagt tcgaatcggc aaatgcctac
     7621 atttggtgat tgggagttaa cagttcttga tcaagcgtac gatcaaatag attatttgtc
     7681 tctgcatcaa tattatggca accaaaaaaa tgatctttct cattatcttg cacgttcctt
     7741 agacatggat cggttcattt ctgaagtgac agcgatgtgc gatacagtca aggcgaagaa
     7801 acatagtaag aagcagatca acctgtcctt tgatgaatgg aatatttggt atcattcagc
     7861 ggaacaagat gagcaagcag cgccttggca gattgctcct cctttactag aagataacta
     7921 taattttgaa gatgcattac tattaggctg tctattaatc actttattga aacatgcaga
     7981 tagagtaaag attgcttgtc ttgctcaatt ggtaaatgtt attgcaccaa tcaggacaga
     8041 aacaaacgga gaagcttgga gacagacgat ctattatcca tttatgcaag ctgctgtgta
     8101 tggacaagga gaggtgttaa gcccacaaat tcgttcagca aactatgcga cggaagattt
     8161 tgaagaagtg ccgtatttag aaagtatagc cgtaataaat gacaaagaac ttgtcatatt
     8221 tgcagttaac cgtggagaag aagaaatgaa tctaactgtt cacttgtctg aattatcatt
     8281 agagggagtg attgattttt ctgaaatgag tggatttgat acaaaagcaa ctaatcttcc
     8341 taaaagtgaa caagtaaaac caagtcattc aacaaatgta aaagctgaag atgggaaaat
     8401 caattgttta ttgaaaccgc tatcttggaa tgtggtcaga tgtcgtttat cagatgaagg
     8461 aggaacaaaa aatgaaaggt aaaaaatggt taattggctc tttgttggta gctgcaacag
     8521 gtattttagg agcttgcggt ggcggaagtt cagcagaatc tgattctgat aaagaagtga
     8581 ctttgacttt ttggaatggg tttactgcat ctgatggtga aatattgcaa gaaatcgtta
     8641 atgaatttaa taaaacaaat gataaaaaca tcaaaattga aatggatatc atgacttggg
     8701 ccaatttgaa tgaaaaactt cctacagcga tttcttctaa aaaagcacct gactttattg
     8761 cattaaatta tccagatttt gcacagtatg tagaaaacgg agcggtacaa ccattagacg
     8821 atttctggga atatgatgga gtagataaat ctgattttaa agaaacggct attgaattag
     8881 gtgaagtaga aggtaaacag tattttgtcc ccatgcaggt acaaggcatg tatatgtatt
     8941 ggaataatga tttgtttaaa caagctggat tagatactga acaaccacct actacttggg
     9001 aagaattgac agaaatggca ccaaaattaa ctgatagtag taaaaatgta tcaggttttg
     9061 tcttcaataa ggatggaact gcaccgttat ataactggat gttagcaaat ggcggtaaat
     9121 tagtgaacga tgattatact aagtctgaat ttgcgtctcc tgagaacctt aaaacgttaa
     9181 aagcgatcca aaaaatgatc catgtagaca agactggtcc agaatcgatt tctggcgccg
     9241 aaatggacaa tttaatgaat gctggtcaac tagccattga aatcaatggg ccatggttaa
     9301 ataacggatt gaaagcaaac gagattgatt ataatgtgac taccttacca caagggagtg
     9361 attcagacaa aaaaacagct attttagatg gcgtaggata tgccattccg gcaagtacag
     9421 ataataataa aaaagaagct gtctatgagt ttttgaaata ttggaataca actgaaattg
     9481 gtaaaaaatg gagtatcgaa aatggttttc ctgcctactt ggattcagta gcaaaagatg
     9541 aagaagtaaa aaatgatcct attgtatctg aattatcgaa acaaatggac tatgcagagc
     9601 catatttacc aggttttacc caaatctctg cgatcaataa cgatatcatc aatccactga
     9661 ttgaaaaatt attagcagga gaagatcctg agacactcat gaacgatgca gatgatgcca
     9721 tcaatcaatt actgcaaaaa tagtacagaa ctttatacag gatagactgt tggcaagata
     9781 cagatatctg aatgatcgac ctagtatttg cagataggtg ttattgcaaa aatcacagcg
     9841 attgttcgga tatcttattc agattcgttt aaaccaagat tgtatatttt atcaaaatga
     9901 aagatgaggt ggactatatg aacggattca ccaaatattg gaatcgtcct gatcgtgctg
     9961 cgtatttgtt tttaactcca tcttttttag ttttattatt atttactgtc ataccgcttt
    10021 taggaacatt tgctattagt tttacagatt tgaatatttt ctttacatca agaaattttg
    10081 taagttttga aaatttcaca cgtatattta gtgatgaacg agcagttaat tcgttgattc
    10141 attctttata tttcaccgta ctcgaaacgc caacacaaat tatcgttggt ttattagcag
    10201 cagtagcatt atctaaaaat accagattca atcgtttttg tcgttcgaca ttttacattc
    10261 cagttatttg ttcattgacg tcaattgcga ttattttttc tatgttgctt gatccgaatg
    10321 taggggcgat tccttacttg ttttcacaag tcggcttgcc gatccctcaa ttttttcgcg
    10381 atccaaccct tgctatgccg acggtagcca tcatgactgt ttggaagaat tttggtgtta
    10441 ctatgacgat tctcttgact gctattcagg gaatctctcc ctctttgtat gaatctgctc
    10501 aaatggatgg tgcaacaaat cgacagcagt tttttaacat tacgctacct cagatcgttc
    10561 cctccttagg tttttgtatt ttaacgaatc tgattggttc gatgcaagtg tttgaccaag
    10621 tatatgttgc aaccgctggt ggcccacaat ttaagacaga aacagctgtt caatatattt
    10681 acagtcgagg atttacagca ccttatgaat tgggctatgc atcagcactg tcttcggttt
    10741 tgtttatcat tgttgcaact atagctatct ccactaatgt gtacatgtca cggaaagaaa
    10801 aacaaatgaa ataggaggga aacctgtatg gcaccagaaa gctcgattac agttactgtg
    10861 aatagtcgaa aagcagttag tggtaaaaaa atcattcgaa cgattagtat gctagttata
    10921 ttgttggtta ttgcgtttcc ttttttgtgg ctgattattt cttcttttaa acacgaaaaa
    10981 gacattatct catttccgcc tagaatattt gcagatagct ataccctaga taactatatt
    11041 aaggtttgga caacgatccc tttattagat tacatcaaaa atacagtgat ttttgctgga
    11101 gggacagtga tcacttctgt attctttgat tctcttgcgg ggtatgcatt tgctcgtatg
    11161 cgtttcaaag gaaaatcagt tttgttctat ttcgttttgc tgacaatgat gatcccattc
    11221 caagtcttca tgattcctct atttattgaa gtcaatttgc ttggcatgct tgatacttat
    11281 gcaggtttga tcattcctcg tatgacaact gcatttggta tcttcatgat gcgttccttt
    11341 tttattactt taccagattc tttggaagag gcagcacgca ttgatggatt aagtgagttt
    11401 aatatttttt tgaaaatcat gttgccattg agcaaaccta ctcttttatc attagggatt
    11461 ttcacgctga tgaatagttg gaatgacttg ctttatccat tgatattgac aagtagttca
    11521 aaaatgagaa ctttaccagc agggttagct ttatttacag ggcagaacat ttctttttat
    11581 ggacctgtaa tggctggaac ggttatttca atgctgccat tactagtggt ttatattttt
    11641 gcacagaaat attttgttca aggaacagct atgtctggaa tgaaagagtg aggaagatga
    11701 aaatagttga gactaaactt tatggacagt tgattgtggt ttctgattac atcttattag
    11761 gtattctctg ggtaatcgtg tgtttgcctg ttattactat cgtgcctgct acatgtgctg
    11821 ttttttatgt aatggcgcaa tggaaaggtg agggatcagg aaatatcatt tattttttct
    11881 ttcaaggaat aaagcaacat tttggtaaga acttgttcct tagtctgtta acgatcatta
    11941 tttattttct tgttaatcaa ttattagaag aacagacaat cgtactttca attagtggat
    12001 atgtagtttt acttatttat tccatgtttc ttttatcctg gatatatgtg atttctgaaa
    12061 acaaagatat cacattttta caaatttttg aacaaagtgc atggaaagta ttttttgatt
    12121 ttttgagaaa tattctttgt tgcgtattgt tattaggatt tgtactgctc atatttcttt
    12181 tccctccttt tatttttatc tttgctggtg gcgtatggaa acttgtatct attatcttga
    12241 ctataggaaa tgaaaggtag ggaaagctgt tggacgaaga agtgaaagta aaaatcagag
    12301 aggattttac ggtaggagag atcagttctc ggctgtttgg ctcatttgtt gagcatatgg
    12361 gaagtgtcgt ctatacaggg atttatgaac cgggccatga aacagctgat ggtgacgggt
    12421 ttagacaaga tgtcctctct tatgtaaagg aattagaatt aggtgtcatt cgctatcctg
    12481 gaggaaactt tacatctggt tatgattggg tggatactgt aggtaggaaa gaacaacgac
    12541 caaaaaaaaa ttgatttagc atggcgaggt atagaaccaa attcttttgg actccacgaa
    12601 ttttttaaat ggatcagtca tgtacaagcg aaaccgatat tcacggtgaa tttaggaaca
    12661 aaaggaattg atgaagcaaa aaatttagta gaatattgta attttccaaa aggaaccttc
    12721 tggagtgatt taagaataaa aaatgggcaa aaaaagcctt ttggcgttaa attatggtgt
    12781 ttagggaatg agctagacgg tccatggcaa gtgggggcta agagtgctta tgaatatggt
    12841 aggttagcaa atgaagcctc aaaagtcatg aagttagtgg atgattcaat tgaaacaatc
    12901 ttagttggaa gctctacgcc acgtttacca tcgtaccctg aatgggatcg tatcgcatta
    12961 gaagaagctt atgagaatgt agattattta gctttgcata actatattga taagtatgat
    13021 gaagaagact tgactaaacc tcctaaaaat gagcgtgatg atacagcgac ataccttgca
    13081 aaagcttatc gattcgatcg tcaaattgaa gaaatcgttg ctacttgtga ttatgtaaaa
    13141 ggatatttga gaagtgagaa aacaatgtat ctagcgtttg atgaatggaa tgtacatgcc
    13201 caaccggaaa aaagacatag ggattttgaa gtcggtagtc ccatcgattg gtgttacttt
    13261 actatggaag atacattatt atttggttca ttagggctgg ctattttacg acacgcagat
    13321 cgcgtaaaga ttagttgtca atcattgtta gtaaacacaa tacctttaat actgacagat
    13381 gctaaaggac aagcatggag gaatccaact tattatgtga tgcaacactt atcgaaatat
    13441 gctaaaggtc aagtattgaa acaacaaatc atttgccctg tttatgattg tgaaaagata
    13501 aaaaatgttc cttatgtgga cagtgtgatc gtagataacg gcaatcagct gactattttc
    13561 ttgatcaatc gcacaaatga acaacaaaag ataacgattg aacatgattt caatttgcta
    13621 gatcaaggag agcactacac attaaccagt agcgatctgt tggacaaaaa tacacgtgaa
    13681 gaaccagaaa aaataaaacc tgaaataaac tatttttcaa tagaaaaaca tgacgaatta
    13741 aatgtaggga tagaaaaata ctcttggaat gtaattagaa taaagaaaat gtgaggaaat
    13801 ttgttatgat gaagacactt tataataatc ctatcgttcg gcaacgggca gatccttggg
    13861 tatataaaca tacagatggt tattattatt ttacaggatc ggttccaggc taccaagtga
    13921 ttgaattaag aagggcaaag tcactgaatg aattagaaca tgctgcaagc ctgattgtat
    13981 ggcaggctca tgaagaagga ccgatgagtg aattgatctg ggcacctgaa gtccattata
    14041 tcaatggaaa atggtatatt tactttgctg caagcaatca taaaactata cgtgatgaat
    14101 ctcatcatca ccgcatgttc gtacttgaaa atcagcatat agatccaatg aatacagatt
    14161 ggatagaaaa gggacaaata aaaacgaaaa gggaaacttt cagtttagac ggaacaactt
    14221 tctcttctca aggaaagcta tattatgtgt gggcccaaca agatcctgaa attactggga
    14281 attctaatct ttatctatcc gaaatggata atccttggac gttaaagggg aagcagatcc
    14341 tcctttctat acctgagtat gaatgggaaa aacggggttt ttcagtaaat gaaggtccag
    14401 cagtccttac tcgcaatggg aaaatattca ttacgtattc tggtagtgca acagatgaaa
    14461 actatgccat gggcctgctg tgggctgatg agcacaagaa tcttttatta aaagaatcat
    14521 ggcataaatt atcggagcca gtattcgttt cttctgaaaa aaataagcaa tttggtcctg
    14581 gtcacaactc gtttacggtt tcagaagacg ggtgctttga tattttgatc tatcatgctc
    14641 gaccagagaa gaaccaaaaa ggggatccgt tagacaatcc taatcggcac gcgaacgcgc
    14701 aatgctttac gtggaatgag caaggatttc cagaatttgg tgaacctgtt ccgtatgcct
    14761 tatgagcaca agaaaccttg atttttgctt gaataaacct tgcagactcg ttatgatata
    14821 tgtacgtaca aataaaaagg ggcaaggcaa atggcaaaat atcaagatat tgcagaagag
    14881 ataaaacaaa agatccttcg tgaggaatac gaagtaaata caaccattcc ctcagaattc
    14941 aagctgcaag aaatatatga cgtcagcaga catacgattc ggcaagcaat cgctgtgttg
    15001 gtaaatgagg gctatctgcg taaagaaaaa gggtcgggca catatgtaga tgacagctat
    15061 aagaaagaaa aaacaccatt gaacagaaca aaaacgatcg gtgtcatcac cacatatctc
    15121 tctgattata tttttccgtc gattatccga ggaatcgaaa atgaattacg agaatctggg
    15181 tattcattgc ttttagccag tacgaataac gatcacgaat tggagaaaga gtgtttgaag
    15241 aaaatgattg cttatggggt agatggtttg atcgttgagc ctacgaaaag taatcagtat
    15301 aacccaaact tagcacttta tgttgctttg agagaacaag gcattccgat ggtgatgatc
    15361 aatgctgctt atgaagagtt aaatgcctct tatatttgtt tagatgatgt gaaagtcggt
    15421 tttatggcag cagaatattt gataaaaaaa ggacatcagc gtctgttgtt catcacaaaa
    15481 atcgacgatc ttcaaggaaa atacaggatg aaaggtttta tcagcgcgtg tgaacagtat
    15541 aatgtgcgtt ttttgacgga agatatcatt actttcacca cggaaacacg agaaacaatc
    15601 gctgaaaaaa cagtacaacg gttgaaagaa gaacaagctt tagatggcat catttgctat
    15661 aacgatcaaa tagctagttt aatgctagat aagttgacag aaaatggcta tcatattcca
    15721 gatgacctat cgatcgtagg aacagacgat tcttctttaa gccatttagg gaataaaaaa
    15781 ttgacaacat tgacgcatcc aaaagaaaag ttgggacaag atgccgcacg gtggatcgtc
    15841 acaacgattg aaaaaggaca ggcacagcca aatattctct atgaacctgt actagttgaa
    15901 cgggaaacag tgaaagaaaa cgaaaaataa gagacgggtt tttaccgcct cttattgctc
    15961 ctacggttac tcgtcgcaat gtttgtgaga gcttttgtca tcgctcaagg ctgaacgttt
    16021 ctttcaccac ctctccacta aacggtgttc tcctaccaac tggtcacaag tcacaatatt
    16081 catcaaacat gagaagctgt tttttgaaat tgttccttgt gactggaaac taagcggttc
    16141 cttcttattt cttgaagctg accacttctg tcacaacctc tttaagaatg atggatagaa
    16201 atacgtttag gataagtttt tttagatgca ctttgttcga tttcctctaa agatttccca
    16261 cgagtttctg gtacgatctc ttgaataaat aaaaccccta aaaagcagat gatcccaaaa
    16321 atgccgaaaa cgatttcttg agacatagaa gcagtcatca caggaaaaag caagccaact
    16381 aggaaagaac caatccaatt aaaagaagag gctaggccag atgctcgccc gcggacagct
    16441 agtgggaata tttcgcctac gattacccat gtcagtggag cccatgtgca agaataaaat
    16501 gcgacataaa tacataaaaa caagagaatc aacaacgaat ttgtatgagc atccagtaca
    16561 gttcctaaaa cagctggtag gatgaaagaa agtcccatca cgctaccgcc tattttcaat
    16621 aaagttcgcc gattgaactt gtctgcaata accaaaaaga gcaaagaacc agcaactaaa
    16681 atgattcctt ggatgatcgg ccacatcaat gcatcgctcg ctgcatgccc agtcgctttt
    16741 tctacgatca gcggaatata gtaaaaaata gcattagctc cttgaaattg ctggaatact
    16801 gctacaccaa taccggcaat gactaaggaa cgatattttt ctaaaaacaa agtatgccaa
    16861 gaagttccgg ttgtcttttc ttgtttgaca ttcagctgga tttgttcgaa ttcctgttca
    16921 gcttcttttg gtgtccgaat atagttcaat actctttttg cctcttccaa gcggccagat
    16981 tgcataagaa aacgcggtga ctctggtaga gctaaaacac cgaaaaataa aatcagcgcg
    17041 gggacagcag cgagccctaa catcacgcgc caagccatcg tttcaggcaa cccttttagc
    17101 aaataatctg caacatagga aaggagcatg ccagaagcaa tcatcacttg attgatccct
    17161 gacaatcttc cccttagtct agcaggagcc atttcagaca tataagcagg aactagggca
    17221 gatgcagcgc caacagctaa acctagaaga atccttgctg caatcagaaa ataagaacca
    17281 ttgtgagggg cagatgcaga aaggacagaa ccagccataa aaataagtgc agaaagaaga
    17341 atcatttttc gtcgtccaag cttatcagaa agaagaccag ataaagagcc gccaaggatc
    17401 gcacctaaca tgaccgaaga cgtgatccat cctatcaaag aagctttacc tgccaaaccc
    17461 caatcatgct gtaaaaaagg taaggcgccg gtcataacac caatatcata tccaaataga
    17521 atgccgccaa atgcgccaaa aaaataaata aacgatgagg aaactttttt ttcttttttc
    17581 ataagtattc tctccttcta gaatttcgag aaggcggttg atcagatttt gtaagcaatt
    17641 tcattttata acttatgtac gtacaaaaca agtgtttttt attgtattct tgtaaataac
    17701 tgcttgacat gtacgtacaa aagtatacaa taatcattgt aagcgctact aaaaagacgg
    17761 tttgatcagg tttgacgaga aggagggagt taaatcatta tgaaggaagt aaatgcgcaa
    17821 agtatgttag atgtcataga tggtcgtaca tctttaggga ttgagtttgg atcaactagg
    17881 atcaaagctg tattgattga ttcatctttt actcctattg ccgctggtag ttatgagtgg
    17941 gaaaacaaac tagaaaatgg tatctggacc tattcattag aagatatttg gaaaggactg
    18001 cgcgtcagtt accgaaaaat ggcagcagaa gtattcgaaa tctatggcga gaatcttacg
    18061 acaatcgggt cgattgggtt cagtgcaatg atgcacggtt atctagcatt tgatagcaag
    18121 gaacagttac tagtaccatt tcgtacttgg cgaaatacaa tcactgaaga agcagaacga
    18181 atattaacgg aaaaatttca atacaatatt ccgcagagat ggagtatcgc tcatttgtat
    18241 caatctattt tgaataaaga agaacatgtg aaagaaatta attttttgac aacgcttgca
    18301 ggttatgtcc attggcagct tactggtgag aaagtactag gcgtaggaga tgcatcaggc
    18361 atgtttccta tcgatccttc aacgaaaacc taccatcaag aaatgaaaaa tatttttggt
    18421 cagttaatcg aaaaagcgca cgtagcgatt gacttggatc acattttgcc taaagttcta
    18481 gtagcaggag aagaagctgg caagttaagc gaatcagggg cttatctgat cgaccctacg
    18541 ggaaatttga gaccaggaat cccggtttgt ccgccagaag gggatgcagg aaccggtatg
    18601 gtcgctacga acagtgtagc caaagggaca gggaacattt cggctggtac ttccgccttc
    18661 gctatgatcg tattagaaaa agaactagaa aatgtccatc cggaaatcga catggtcact
    18721 acaccttcag gagaactagt aggcatggtt catacgaata actgctcttc ggatatcaat
    18781 gcttgggtca gtatttttga agaatttacc aattctttgg gtatggagat aagcagagat
    18841 aaattattca cggtattatt caacaaagcg ctagaaggcg atacggattg cgggggatta
    18901 ctttcttatg gttatttttc cggtgaaaac attacaggag taaatgaagg aaggccatta
    18961 tttgtacgta caccaggtag ccgttttgat ttagcgaatt tcatgcggat ccatttagcc
    19021 agtgcttttg gtgcaatgag aatcggaatg gatatattga aatctgagaa tgtccaaatt
    19081 gatcgtttag tggggcatgg ggggattttt aaaacaccag aagttggaca gcggattcta
    19141 gcatctgcta tggaggcacc agttacagtg atggacacag ccggagaagg tggagcgtgg
    19201 ggaatcgcgt tattagcagc ctatatgatg gatcgacatg ggaagtcttt agaaaacttc
    19261 ttggcagaag acgtgtttgg acaagaccaa ggaatcacca tcacaccttc agcagaagaa
    19321 atagcaggct atcagatatt tatgagacgt taccgtgagg ggatcgacat tgaaaaaaca
    19381 gcgattcatc aattgcaaga aaaaggagtg atagaaaatg ctggaacaac tgaaagaaga
    19441 agtataccaa gcaaatcttg acttgcctaa acacggactc gtcaaatata catggggaaa
    19501 tgtgagcgct tttgatcctg aaacacgata tttcgttatc aaacctagcg gagtcagtta
    19561 tgaagagatg acagcagacg acatggtcgt tgtggacttg gacaatcata tcattgaagg
    19621 taaactcaat ccctcttcgg atacgcctac tcatgctgtt ttatatcgtt catttcctca
    19681 aatcggagga atcgtccata ctcactcgac atgggcgacg atctgggcac aagcaggatt
    19741 ggatgttcct gcgatgggga cgacgcatgc agatactttt tatggatcgg taccttgtgc
    19801 tcgatttttg acacaacagg aaatcgacag cgggtacgaa tatgaaacag gaaaagttat
    19861 catagaaaca ttcaaagaac gcaaaattga tcccttagca attccagggg tacttttaca
    19921 cggacacggt ccatttactt gggggaaaga tgcgcaaagt gcggtaatga acgcagtcgt
    19981 actagatgaa gtatgcaaga tgaatttatt taccagacag ctcaattcat tttcagaaga
    20041 gctgcctcaa cggattttag ataaacatta tttacgaaaa cacggaaaaa atgcgtatta
    20101 cggacaaaac aaataaaagg agtggctaat tatgttgaat attggagaaa aagaattttg
    20161 gtttgttgta ggatcacagc atttatatgg ggaagaagca ctgcgagaag taaagaaaca
    20221 tgcacaagaa atggtcgatg aactgaatga aaatggccaa ttgccttatc caatccgcct
    20281 tcaagaatta gcagttactg ctgatacgat cacaaaaatc atgaaagagg tcaattatcg
    20341 agaagaagta gcaggtgtga tcacttggat gcatactttc tcaccagcaa aaatgtggat
    20401 ccgcgggaca aagcttctac aaaaaccatt gcttcattta gctacgcaat ataacgaaag
    20461 cattccttgg aaaacgatcg atatggattt catgaattta aatcaatccg ctcatggtga
    20521 ccgcgaatat gggttcatca atgctcgatt aaataaacaa aataaaatcg tcgttggcta
    20581 ctggaaacga cctgagatcc aaaaagagat tgctgattgg atggatgttg cagtagccta
    20641 taatgaaagt tttggaataa aagtggctcg ttttggtgac aacatgagaa acgtgggtgt
    20701 cacagaagga gacaaagtgg aagcacagat tcaatttggt tggactgttg attattttgg
    20761 tatcggagat cttgtacaag taatcgatcg cgtatcagac gaagaagtcg atcaattatt
    20821 tgaagagtac aaggaattgt ataccttcga ctatggtgat tatgaagaaa agacatggga
    20881 ggaacatgta aaagtacaag ctcagcagga aatcggtatt cgccgtttct tagaagaagg
    20941 aggctataac gcttttacga ctaattttga agacctgtat ggaatgaaac aactacctgg
    21001 actagctgta caacgtttga tggcagaagg atacggtttt gctggggaag gcgactggaa
    21061 aactgcagcg attgatcggt tgctgaaaat catggctcga gggaaagata caggattcat
    21121 ggaagattat acttacgagc tagctagtgg ccaagaagca atcttggaat cccatatgat
    21181 ggaagtagat ccgacgcttg ctgcgacgaa accaagaatc gttgtttctc ctttatcaat
    21241 gggagataga gaagatccgg cgcgtcttgt atttgatggg aaagcaggag aaggggtagt
    21301 cgtatcgatg gcagatttcg gtacgcatta taaactgctg atcaatgaag tagaagcctt
    21361 tgaaccaacg actgaagcac ctaatttacc tgttgcacgt gtgttatgga aaactaaacc
    21421 gaatttccat gaaggtgtcc attcatggat tcaggcaggt ggcggtcatc ataccgttgt
    21481 ctcgctaaat ctaacaacag atcaaattga aacgtgggca aaattagttg agttggaaac
    21541 agtggttatt agataaaaag aggttgtgaa aaagccactt agttttaaga aataagaaga
    21601 gattgacgaa atttgctttt caactttttt gaaatttagt cttattatca atgtcagcta
    21661 acggaacacc atttatcaag atatctcacc ctcaatattt tgctctcgaa aataaggctt
    21721 atggcagtaa cttgtcatag ccttattttt atcactcagc ctactctgct ctaactgctc
    21781 tttttgcttc taggaagtca aatggtaaac agttgtctaa ttgcagaaat ttcgagatac
    21841 ttgttagtaa ggagggggtt ttatgtatca cgttcatttg attgatgcta ttccagaaca
    21901 agagatagaa aagttacaag gggaatattt caaaataata gatgatttgc cagccgaagg
    21961 aatagttatt cgtagtacga aagtgaaaga tgaatggctt acaccagatt tgctagcaat
    22021 ttcaagagct ggtgttgggg tgaacacgat caacgttgaa aaagcttctg aaaacgggac
    22081 gatcgtcatg aatacaccag gtgtaaatgc taatgcagta aaagagcttg ttttgtgctg
    22141 tttgctctta tcttcccgtc cgataatcga agcaagtcgt atggtacaaa cactaacagg
    22201 tcctaatatt ttagaacagg cagaaaacaa aagaagtgct tatgtgggac gagaattgca
    22261 aggcaaaacc atcggtcttc ttggtcttgg agcgataggc acaaaagtag cgctaagttg
    22321 ctatagctta gggatggatg tcttgggtta ttctatccgc gatgcccagt tggattatgt
    22381 tcggcaagca gatttagaaa cagttctttc tacctcggat tacatcgtag tcatgctgcc
    22441 gttgacagaa gatacaaaag gattgatcga tcaggcgaat atagaaaaaa tgaaaaaaga
    22501 tgctgtactt atcaatgtag gaagaagtga aattgttgat aaatatgctg ttatgcaagc
    22561 attggaaaaa caacatctgg ctaaatactt gactgatttt ccagaagaag agttcttaga
    22621 aaatgaccgt atacttatgt tgcctcatct agggggatcg actcaggaag ctttggcaga
    22681 cagcggacgt ttggcagttg aagccttaaa agattatctt ttgttcggta cggttagaga
    22741 agcggtcaat tatccatccg cacgaatgtt tttccaagcg ccgtttcgat tcactatatt
    22801 ttatcaaaag aaaaccaata tattagcaga gatattttct ctgctgaatg agaacgagct
    22861 agcgatcgca gatatatcgc gtaaccataa gaatgagcat gtctacatat tgattgacat
    22921 agacagtact gattttgaac agctcaatca agtgaaacaa cagctagaaa aaatttctgg
    22981 agtacgaaag gtccgcttat taaagaaacc agaaaggcca taaagaaaga gaatatttgg
    23041 atttcattaa ttcttctttc cgttctttac tttcgaattt cgtacaacta aaataagaga
    23101 agcaacattc ttttattgat agaaatgaag gagcaagcat ttgaaaagat atataagcag
    23161 actttcgacc aaacagaaaa agattttaaa aggaacagca gcaatcatca gtatcttttt
    23221 tcttgttgtt tttagtaatt tatttttgca gtggtgtcaa aatgatctat ccgtagacct
    23281 tgcgttgaaa tttgcttttt cttggcatac ggaaaaattc ttcttagcct gtctcgtctt
    23341 gctgattatt cttatttttc tgattgcact agcaggatct gtccctttag gttcccttac
    23401 gtatgtcgta gccatcggtg tattaggttt tgcaaattat atgaaaatga gttatcggca
    23461 agaaccgatt tatccagacg atttgaagat gatcactgaa atcggattgc tgaaagatat
    23521 gaccggcact atgctgttta cagtgatctt agctgccgca ggaaccgtga taggattgtt
    23581 ttgctggtac atgtttcgaa gcctgaaaaa aggaaggcga ttccaattga tccgattaac
    23641 gacactgctt gtggcaattg gactgctagg gtatatcagt aatttcaaca atcccgataa
    23701 tttgttgcga aaagcatata ataaaacggc actatggata ccttacagcc agaaaatgaa
    23761 ctactataac actggtttta tcggtgggtt tttgtacaat ctgaaggtag aaccaatgga
    23821 tgaacctgaa ggatattcga aagaaaagat caaagaaatc actgaaaaat accagaagct
    23881 agccgatgaa aaaaataaaa ctgtagagga agagtcgccg aacatcgtgt tcgtgatgag
    23941 tgaaagtttc tcagatcata gtcgattaaa cggcgtagaa gtatctggtg agccattggc
    24001 agattattat gaagtagctg atcaaacata tagtggcaat atgctttctc aaaactatgg
    24061 cggtggaaca gcaaatatcg aatttgaagc attgactggt ttttctatgg cgttatttaa
    24121 tgcgcaactc accacccctt acacgatgct tgtaccaaaa atggatcagc ttccttctat
    24181 cgtttcagcg ttaaacgcac aatcatacca gacgacagcg atccatccat acaatacttc
    24241 gatgtataaa agagaggatg tttatcagac attaggattt gaccagttca tcagcgagcg
    24301 tacgatgacc tatacggata cgatagagaa taatccatat atttctgatg agtcagctta
    24361 caaagaagta atgacacttc tcaaggaaga aaaaacacct cagttcattc atttagtcac
    24421 gatgcagaca catatgccct acgatggaaa gtatgataat ctagactatc ctgcaaaaac
    24481 ttcagatgga agtgggacac ttgacttaga gaattatctg caagatattt cctatagcag
    24541 cacggcactg aagcagttca cggaagaatt gaagaatctt tctagaagga ctttagttgt
    24601 tttttggggg gatcatcttc cagggatcta ttcagatacg atccaagcaa aaaatgacaa
    24661 acaaacattg catgaaacac aatttcttat gttcgacagt aaaggagaat tagaaaagca
    24721 aacgactcac gatgcaatca ccagtccgtt ttattttgca gcgaatctaa tggaacagac
    24781 gaatcagaca acaaatggct tttatcagct gcttttatca ttagaacagg aacttccggc
    24841 gtttgaacga gaactgtact atcaaaacgg acagtggtat aaagaagcgc aattcaatcg
    24901 ctcgcaacaa gagatttacg acgaatatca attgatccaa tatgacattg tcgcagggaa
    24961 acaatatagt ttagcagatg gtttctttga gcacgaataa gaatggcgag ctgtatagaa
    25021 accattaaat tttttcaagt acttgtcatt tgaggataaa aacaagtaga ataaataata
    25081 acaacaatgg agaaagtagg aaaacgaaca tgttaccgaa tattggctat tatgtgcagt
    25141 ccatcgatca aatcgtcaag gaaacagaag caatcggaga aggaatgcat ccgaaatatg
    25201 aagtgatcag aaacgcaatt gatgaaggaa aaacaagtga actggacaaa tcgactcttg
    25261 aagagatcat cctgttattt gaagaaggaa caaaaaaata tcgggcaatg cttatgaaag
    25321 tcagtacttt gcgtccacca gcaaaagcaa tgggcattca taaaaatttt gaaaaagctt
    25381 atctttcata tgtagcaggc tgtgaagaaa tgatccaaag tatcgatctt gaatcagggg
    25441 tagatgaaga tctattcaat acatctgaag caaaacaaga ccaagcgaca gaccaattga
    25501 cagctgccat ccaaaaaatg actaatttgt tgttgaaaaa ataaaagaaa tctttgataa
    25561 caataaaaac tagagttatc taatataaat gtaatattac ctttatgata gagtgatcaa
    25621 aatcctttat gataatgaca tacctataac caacaacaac cttgtactta ctcaaaatcg
    25681 agtaaatact ccttttccgt cgagtctaac catctcggcg gttttttatt tgtagaaaag
    25741 aacatcagga tagacggaaa agtcttgtcc tgatgttctt ttcagatagt atggaaagct
    25801 gattagtttt ctacattctg tacatattgt tcaaagtagt tctgtaaatc ggctttgagt
    25861 ttctgatatt cctcttcgct atactcttta tcttcgattt tcccatcaaa gaaaggaata
    25921 tttagttctt cccgtagtct gtctaatacg tcttgctgat tcatttcatt ctccttttct
    25981 tactaacgat caaaataaac acagacacat tctggtacat agacatcttt tttcagtaat
    26041 tccagtaatc ctgtttcagg atgacggcga tataatgtca gattatcggt gttctgattg
    26101 gcacacacga caaaattatt gcttggatct agtgcgaaat cgcggggaaa atccccttct
    26161 gttgagatga tttgcttgtt ttgaatgtgg gaaccatctt caaaaactgc gaatacagca
    26221 atcgagttat gtcctcggtt agaagcataa aggaacttgc catcatttga aatgcggata
    26281 gctgcccctc cattttcacc gtcgaattct tctggcaagg tcgatatttt ctgtaattct
    26341 tcaaaagcac ctgtttcttt attataagaa agcacgcgga ctgtgctgtc taattcacca
    26401 aataaataag cgaattgtcc atttggatga aagacaagat ggcgagggcc tgttcctggc
    26461 tctgcagtga aggtagttac aagattcaat ttgccgtttt cggacacatc ataagtatac
    26521 acacgatctg taccaagatc acatacgact aatcgttggt caggagtcag atccgtatag
    26581 tggacatgcg catgatcttg gtttttatga ggacctgtcg cttcttgatg gaatattgaa
    26641 tctgctgctt tcagttcgcc atctcctaat atatggtaga catttacttc acctttatga
    26701 tagttcgccc cgtatagtaa ttgacgtgct tcatcaaccg ctacataaca caaaggagcg
    26761 ccagactctg ttaccgcatt caaaaaatga aaatccttat cgtacgcacc ggcaccacct
    26821 aatccatcca cactagtaac tgtgtaaaga ttgccagctt tactttttgc tacataggta
    26881 gggctatttt cttcaacagc taatgaaagg ttggctaatt cttctttttc tgtatcaagg
    26941 tcgatcgtat agattccttt actttcgcga cgcgtatagg ttcctaaaat tatcttttcg
    27001 atcatatgag ctgattcctc ctaattgtaa tgaaatagtt ttcgttttta ttctatcaca
    27061 taattattcc acacggctaa agaatgagaa tgtgttatac tgtttttgaa agcaattaaa
    27121 ggagagagtg caatgattca ggcaaagatt acagaaattg ggacagaagc aataaacaag
    27181 gaagaaccca tgctgatttt gttcgataaa acagcaacag ctgccttacg aaaatattcg
    27241 gtgattcaag aagtatcaaa agacgaacct ttttctttga aagcaggcgg aacaattgct
    27301 tttgatgatc aggtatatac gatcgattat gtcggtccga tggccaatgg gaatttaaag
    27361 acagtagctc acgtatcatt gatttttgat gcttgtccag aagaaggaca aattgcaaat
    27421 ggaatctatc ttcatccgca tagactgcct gctattggtt tgggaagcca aattttttat
    27481 aactaacagg aggaattatg gaaggaaaaa ataagaagaa cagaccggta tcgtggtttt
    27541 ggcgctggtt tttaaataat caagtagtca cagccttact tgttgtattg ttaatattgc
    27601 tgatcgtctt tttatttaca aaagtttcat atttgtttgc accaatctgg caatttttag
    27661 caatcgtagg tctgcctatc attttagcag gaatcttgta ttacttgatg aatccggtag
    27721 ttgattactt tgaaaagcga aaagtaccaa ggctttacag tatcattggg ctgtttgttt
    27781 tggttgttgc actaatcgca tgggggagcg tagtgatcat ccctaagatt caggaacaaa
    27841 cagtgagctt catcagtaac ttccctcaat atgtagatac gattgataat aaattaaccg
    27901 agattttgcg ggacccgctg ttcaaccaat ttcgcgaaca gttggaaaca gcaggtgata
    27961 agttcatgaa ttcggcaggc gatatgatcc aagatatatc taagagtaca gttcagagtc
    28021 ttggaagttt tgtcggcgcg gtagcgacta ttttagtggc tctgctgacg atgccgttta
    28081 tcttatttta tctattgaaa gacggtaaac aattagcacc ttattttgtc aaatttttac
    28141 cgacacgcat gcaaaaaccg acgttgaatg tgctgagtga aatgaatagt caagtttctt
    28201 cttatataag aggacaacta actgtggctt ttgctgtggc aatcatgttt atgattggtt
    28261 ttgcggtcat tggtttagag tatgctgtca cgttggggat tattgcgggt tttttaaacc
    28321 tgatccctta cttaggatca tttttagcga tgattccggc tatcttttta gccattgtag
    28381 caggtccatt catgatcatc aaagtactga tcgtttttgc agtagagcag acaatagaag
    28441 gacgcttgat ctctccatta gtactaggga atcagttagc aatccatcca gtaacgattt
    28501 tattagttct gctgacttct gggaaattgt tcggaatcat cggtgtgatc ttggggattc
    28561 ctgtttatgc agcagctaaa gtagtgatta ctcatgtttt tgaatggtac acaacaatct
    28621 cttctcttta tgaagaaaat ccgttgaaaa agcagaaaga ataacaaaaa gagacagatg
    28681 ggaaaatatc caaggcacaa atgctgcttt gaagattttt tcctctgtct cttttgtatg
    28741 catttatctc aatttttaaa atgatttacc taaagcagca gctttatcta atgcattttg
    28801 catcaactca tctgcacggt cagggtcata atcgattcct tcgatgaata gctgatctac
    28861 ttgatcgatg cctacaaagt ttaaaatacc ttttacatat tgagaggcaa agtcttgtcc
    28921 gttgtataca ccgccattag attggatgtg cagtgctttt ttacctgtca ctaatccttc
    28981 tggcccgttt tctgtatagc ggaacgtttt tccagctaca ttgacggtat cgaaccaagc
    29041 ttttaatttt gttgggatat ttagattcca taaggcgttt gcgataacga ttttatcagc
    29101 agcaaggaac tgatcagtca attcattgaa acgagcaatt ttttgttgtt gactttctga
    29161 taaggaagtg aattctgcac cagcacgtaa tgcttcccaa cctgataata attcttcatc
    29221 gatttccgga atatcatcgc gataaagttc caaaacagtc acttcatcat ctggatgatt
    29281 ttctttataa cttgttaaaa aagacgtcaa tgccttaact gtgcgcgatt cttctgcagt
    29341 taggggatga cctttgacta ctaaaacaga tgccatatgc ttaccttctc cttttattgc
    29401 tatgattgtt ataactatga ctaacaaaat cagtttactc gaaaaagaaa aggctatcaa
    29461 gtcatattga taaggttgaa gaataaataa ctaaaaaaag acagggtata aaagaggctg
    29521 tgacacgatt ttttgtcaca gcctcttttg aatctaacaa gcaatattca agcaacactc
    29581 gactgaagag cttccatccc accaatgata taaacagcac gaaaaccatt agcagtaagt
    29641 tgatcggcta atgcttctga tcgcctgcct gtatagctta gaatataata aagtttcttt
    29701 ggatctagat tttttaattg tcttggcaag atagcagcgg ggatagaaag cgagtggctt
    29761 aagtggtttt ttgtgtaatc taaggtattt cttgtatcta aaacgatgct gtcagcatcg
    29821 agaggcaatt tattgaagtc ttggacacta attgattggg ccatttatct catcctttgt
    29881 ttagtatgtg gctattttaa tgggcaactc tcattaattc aaatggaagg attcctctta
    29941 tcactttttt aatgtatcgg atgataatga aaaaaactat gcaaatgtct tgcaaaacag
    30001 gtaggaggtg ttataatgac atagcgtcat atgacaatgt gtcatcttga aagaaaggag
    30061 aatccatatg ccgacacaga catttttcca tttgcctaaa gaaaaacaaa aaagactaat
    30121 cgaggctgcg agaatcgagt tttctagagt acccttaaaa gaagcttcga ttgctaatat
    30181 cgtcaaatta gcggatatcc ctagaggcag tttctatcag tattttgaag ataaagagga
    30241 tctttatttt tattactttg agacaatgag acgtgacagc tctaaagata tgttacaact
    30301 aatgaaagaa tccgatggtg atctattcaa aggattcgaa tcctattttt ctaaaatgat
    30361 ttatgagatt ttggaaggcg agaatgcttc tttttatcgt aacttgttta tgaacatgga
    30421 ttaccgtgcc tcaagaaaag tcgctcctca tacgagtcgc caaaaaacgc ctcacgatcc
    30481 acataaagaa cacaaacaag atgcaaaaga gttgcttgaa ttgatcgatc ggacgaagct
    30541 gaaagtaaaa aatgatcatg aattggaatt actgatccaa ttagtgatga atactgtatt
    30601 tacaacaatt gcgcattcgt atcgcttatt ttcaacatct gaagattact cagtagagca
    30661 agcaatcagt gaattcaatt tgaaactttc ttggctgaaa aacggagtat ataaatagaa
    30721 agaagggaac tagatgctaa aaatcgtaaa acggatctcc ttgacttcag caattgcagc
    30781 tgcagttttc atggtgatcc aagtactggc agatttatat ttgccgacac ttacctccaa
    30841 tattattgat aaaggggtag cgcaaggaga tgtcgattat atttggcata caggttttgt
    30901 gatgatcggc ttttctttga tcagtatctt agctgctatc gcaaatacat tttttgctac
    30961 tcgtgaatca cagaaactgg gtaagcaatt gcgtacggat gtctataaaa aatcagagag
    31021 tctgacaaaa gatgcgtttg ataaatatgg aacggcctct ttgatcacaa gaacgaccaa
    31081 tgatgtgact caaatccaaa tggttacaca aatgttttta cgtatgatga tcaatgcacc
    31141 aatcacgttg attggcgcga gcatacttgc ttatcaaaaa gatcatcagt tgacaaaaat
    31201 cttcttagtt gtcatcccag taatgattat tttgatcggc ggaatcatgt actttgctgt
    31261 tccattattc aaaagtatgc aaaagaaaac tgaccgttta aacctggtat ttcgagaagg
    31321 attgacaggg gtcagagtga ttcgagcatt cgataagaca cgctttgaag aaaatcgttt
    31381 tgatttagcg aataaagatt atacaaatac ggcaatcaaa gttaatacga tcgtcgcatt
    31441 gatgatgcca atgatgacat tgatcatgag cggtacaaac gtagcaatca cttggtttgg
    31501 tggacactac atagcagata tgactttgga agtcggtaat ctgattgcat ttatgactta
    31561 tgcgatgcag atcttgatca gctttatgat gctttccgct atttttatca tggtgcctcg
    31621 tgcacaagct tcagctgacc gtattaatga agtgttggat gaaaaaatcg gtatccatga
    31681 ccctgaaaat cctaaaacag tttcttttgc tggaaaaaac gcaacattgg ccttcaatca
    31741 tgtcaattat cgatatcatg gagcagaaaa acttgcattg gaagatattg atttccaagc
    31801 caagtcgggc gagatcgtcg caatcatagg ggggactggt tcaggtaaaa cgaccttagt
    31861 caatttgatc cctcgattat atgacatcga atctggttcg atccaaataa atggaacgga
    31921 cattagtgac atgactcaat ataatttgcg agaattaatg ggctttgttc ctcaaaaagc
    31981 agttctgttc tctggaacga tccgagacaa tatgcagtat ggtaagccgg atgcaacaga
    32041 tgaaatgatt tggaaagcat tagaaatcgc acaagctaaa gattttgttt cagagatgga
    32101 agatggctta gatagccatg tggaacaagg aggaggaaac ttctctggtg gacaaagaca
    32161 gcgtctggca attgcacggg cattagtcaa aacagcagat atctatgtgt ttgatgattc
    32221 cttctcagcg ttggatttca aaacggatgc caatcttcgc caagcattga aaacgaatat
    32281 gaaagaaagt atcaccgttt tagttgctca acgagtaagt acagtaatgg atgcagatat
    32341 gatccttgtg ctagatgaag gaaaactggt tggcaaagga acacatgaag aactattagc
    32401 aacaaacgaa acttatcaag aaatcgttca ttcacaacta agagaggagg acttagcatg
    32461 agtaaaacaa acacacgtcc ctctcatgga ccagcaggcg gtccaggaca tggtggacct
    32521 ggcagaaatc tcggtgtaaa aggtgaaaag cctaaaaatt tctggggaac ggtacgccga
    32581 ttgtttggct atatgtctaa acgtatggtt gcgatcatcg ctgtatttgt actagcaata
    32641 gcagccgtga tcttccaaat ccaaacacca aaggtcttgg ggaaagcaac cacagaaata
    32701 ttcaaaggtg taatggctgg cgctcaacag atgcaacaag gccaacaagt aacaaaattt
    32761 ccgatagact ttgataaaat cgcgcagatc attgcgacag taatcgtgat gtatttgatt
    32821 tctgctgtat tcaacttttt acagcaattt attatgacac gtgtttccca acgaacagtt
    32881 tatgaattac gtcatgattt ggaagccaaa atgaatcgtg taccgatttc ctattatgat
    32941 acacatacaa atggggatat catgtctcga gcaatcaatg atatggataa catcgcaagc
    33001 acgctgcagc aaaatttgac tcaatttgta actagtgcag tcacattgat tggggtcatc
    33061 gtgatgatgc tttcaatcag ctggcaattg acgttagtcg cgttattgat gatcccttta
    33121 agcttgatca tcgtgatgat cgtagcgccg aaatcacaag tgtttttcgc agatcagcaa
    33181 aaaagtttag gtttattgaa taaccaagta gaagaaacat atggtggtca taccattgtt
    33241 aaaacattca atcatactga aaaagaccag gaagtatttg aaaaagaaaa tcagaatctg
    33301 tatgccgctg gttggaaagc tcaatttatt tcggcaatca tcatgccgtt gatgaatttc
    33361 gttaaaaatc taggctatgt gttcgtcgct gtactaggcg gagtaaaagt tgcaaatggg
    33421 aacatgacat taggggatgt tcaagcgttc ttgcaatata caacacagtt ctctcaacca
    33481 atcactcaac ttgcaagttt gatgaacact atccaatcaa cagttgcttc agccgaacgg
    33541 gtatttgaag tgttggatga agaagagatg tccaatgaaa aatctggcct tccagaagaa
    33601 aaagatacac catacaaagt tcgttttgaa aatgttcaat tcggctacac accagataac
    33661 ttgctaatga ctgattttaa cttggatgtc aaacctggtg agatggtcgc tattgtaggt
    33721 ccgactggtg cagggaagac gacactgatc aaccttcttg aacggttcta cgatgtttct
    33781 ggaggtagta ttcgttacga tggtgtagat acccgtgatc tttcacgtga tgaattgcgc
    33841 gcacagtttt caatggtact gcaagatact tggctgttta caggaagtat ctatgacaat
    33901 atcaaatatg ggaacgacga tgcaacagac gaacaaatca ttgaggctgc taaagctgct
    33961 cacgtagacg attttgttcg taaattgcca gaaggatata acactgtatt aaacgaggat
    34021 gcaagcaata tttcgcaagg gcagcgtcag ctaatcacaa ttgcgcgggc attcttagca
    34081 aatccagatg tcctgatcct tgatgaagca acatctagtg tagatacacg aacagaaata
    34141 ctgattcaaa aagcgatgaa ccgcctattg gaaaaccgga caagtttcgt tgttgctcac
    34201 cgcttgtcta caatacgcga tgcagacaac attattgtga tgaatcatgg ttcgatcgta
    34261 gaaacaggaa accacgatac attgatggaa aaagacggat tctatgcaga tctttataac
    34321 agtcagtttt ctgaagaaga agcttcatag aatagtacaa atttattatt ttttttaggg
    34381 gctgtgacaa aagtcgtgtc acagtccctt tttccgaata aacggtgttc aatcagctgt
    34441 ccttcggtat tagctcaaaa aacggcaaaa tatgagaagc tatttttccg ttttgtatct
    34501 ctaatactca ggtcaaaccg ctgattttac aacctcttcc ttaaacggtg ttctcctgcc
    34561 aactcctcgg tcacaagtca caatagtcaa caaacctgag aagctgtttt ttgaaattgc
    34621 tccttgtgac tcggagttaa acggcaggct cacaacctct tg
//
LOCUS       DACDYI010000023        34050 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.023, whole genome shotgun sequence.
ACCESSION   DACDYI010000023 DACDYI010000000
VERSION     DACDYI010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.023"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            2..400
                     /locus_tag="IWM52_001086"
     CDS             2..400
                     /locus_tag="IWM52_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP9973352.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            384..1079
                     /locus_tag="IWM52_001087"
     CDS             384..1079
                     /locus_tag="IWM52_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAP9973353.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1673..2560)
                     /locus_tag="IWM52_001088"
     CDS             complement(1673..2560)
                     /locus_tag="IWM52_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAP9973354.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2745..3584
                     /locus_tag="IWM52_001089"
     CDS             2745..3584
                     /locus_tag="IWM52_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP9973355.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3780..4700
                     /gene="sgrA"
                     /locus_tag="IWM52_001090"
     CDS             3780..4700
                     /gene="sgrA"
                     /locus_tag="IWM52_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAP9973356.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSMSSESSTGSTSSESSTGKVN
                     NELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQEKAKETDNRKSDLPETGSR
                     TLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            4998..5354
                     /locus_tag="IWM52_001091"
     CDS             4998..5354
                     /locus_tag="IWM52_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973357.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5357..6514
                     /locus_tag="IWM52_001092"
     CDS             5357..6514
                     /locus_tag="IWM52_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973358.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6540..6980)
                     /locus_tag="IWM52_001093"
     CDS             complement(6540..6980)
                     /locus_tag="IWM52_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP9973359.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7140..7646
                     /locus_tag="IWM52_001094"
     CDS             7140..7646
                     /locus_tag="IWM52_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9973360.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            7814..9727
                     /locus_tag="IWM52_001095"
     CDS             7814..9727
                     /locus_tag="IWM52_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP9973361.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9732..10733
                     /locus_tag="IWM52_001096"
     CDS             9732..10733
                     /locus_tag="IWM52_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9973362.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(10935..11141)
                     /locus_tag="IWM52_001097"
     CDS             complement(10935..11141)
                     /locus_tag="IWM52_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAP9973363.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11787..12635)
                     /locus_tag="IWM52_001098"
     CDS             complement(11787..12635)
                     /locus_tag="IWM52_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9973364.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12799..13701
                     /gene="gnd"
                     /locus_tag="IWM52_001099"
     CDS             12799..13701
                     /gene="gnd"
                     /locus_tag="IWM52_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAP9973365.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            13703..14704
                     /locus_tag="IWM52_001100"
     CDS             13703..14704
                     /locus_tag="IWM52_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP9973366.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14701..14985
                     /locus_tag="IWM52_001101"
     CDS             14701..14985
                     /locus_tag="IWM52_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9973367.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15018..15815
                     /locus_tag="IWM52_001102"
     CDS             15018..15815
                     /locus_tag="IWM52_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9973368.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            15829..16662
                     /locus_tag="IWM52_001103"
     CDS             15829..16662
                     /locus_tag="IWM52_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9973369.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16680..17537
                     /locus_tag="IWM52_001104"
     CDS             16680..17537
                     /locus_tag="IWM52_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9973370.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17559..18068
                     /locus_tag="IWM52_001105"
     CDS             17559..18068
                     /locus_tag="IWM52_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9973371.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18105..18506
                     /locus_tag="IWM52_001106"
     CDS             18105..18506
                     /locus_tag="IWM52_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973372.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18618..18971
                     /locus_tag="IWM52_001107"
     CDS             18618..18971
                     /locus_tag="IWM52_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973373.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19005..19448
                     /locus_tag="IWM52_001108"
     CDS             19005..19448
                     /locus_tag="IWM52_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP9973374.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19465..20550
                     /gene="uxuA"
                     /locus_tag="IWM52_001109"
     CDS             19465..20550
                     /gene="uxuA"
                     /locus_tag="IWM52_001109"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP9973375.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20543..21256
                     /locus_tag="IWM52_001110"
     CDS             20543..21256
                     /locus_tag="IWM52_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9973376.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            22008..22208
                     /locus_tag="IWM52_001111"
     CDS             22008..22208
                     /locus_tag="IWM52_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9973377.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            22776..23339
                     /locus_tag="IWM52_001112"
     CDS             22776..23339
                     /locus_tag="IWM52_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973378.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(23400..23765)
                     /locus_tag="IWM52_001113"
     CDS             complement(23400..23765)
                     /locus_tag="IWM52_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973379.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(23841..24086)
                     /locus_tag="IWM52_001114"
     CDS             complement(23841..24086)
                     /locus_tag="IWM52_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9973380.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(24156..24410)
                     /locus_tag="IWM52_001115"
     CDS             complement(24156..24410)
                     /locus_tag="IWM52_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973381.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(24574..24897)
                     /locus_tag="IWM52_001116"
     CDS             complement(24574..24897)
                     /locus_tag="IWM52_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973382.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            25084..25157
                     /locus_tag="IWM52_001117"
     tRNA            25084..25157
                     /locus_tag="IWM52_001117"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25118..25120,aa:Ile,seq:cat)
     gene            complement(25542..26039)
                     /locus_tag="IWM52_001118"
     CDS             complement(25542..26039)
                     /locus_tag="IWM52_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9973383.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(26079..26888)
                     /locus_tag="IWM52_001119"
     CDS             complement(26079..26888)
                     /locus_tag="IWM52_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9973384.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            27222..28079
                     /locus_tag="IWM52_001120"
     CDS             27222..28079
                     /locus_tag="IWM52_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP9973385.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            28072..28974
                     /locus_tag="IWM52_001121"
     CDS             28072..28974
                     /locus_tag="IWM52_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP9973386.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            29007..29357
                     /locus_tag="IWM52_001122"
     CDS             29007..29357
                     /locus_tag="IWM52_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9973387.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            29391..30329
                     /locus_tag="IWM52_001123"
     CDS             29391..30329
                     /locus_tag="IWM52_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP9973388.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            30407..31300
                     /locus_tag="IWM52_001124"
     CDS             30407..31300
                     /locus_tag="IWM52_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9973389.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            31338..31901
                     /gene="amaP"
                     /locus_tag="IWM52_001125"
     CDS             31338..31901
                     /gene="amaP"
                     /locus_tag="IWM52_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP9973390.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            31915..32103
                     /locus_tag="IWM52_001126"
     CDS             31915..32103
                     /locus_tag="IWM52_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP9973391.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            32116..33018
                     /locus_tag="IWM52_001127"
     CDS             32116..33018
                     /locus_tag="IWM52_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9973392.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKNGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            33050..33292
                     /locus_tag="IWM52_001128"
     CDS             33050..33292
                     /locus_tag="IWM52_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9973393.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(33548..33919)
                     /locus_tag="IWM52_001129"
     CDS             complement(33548..33919)
                     /locus_tag="IWM52_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973394.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT        11842 a         5405 c         6838 g         9965 t
ORIGIN      
        1 gatgacgatc tttgaaacgg ttgtaacaga tgttggtata gaagcagaag tattcaggaa
       61 agaaaaaata atgattttat ttggagatga agcaccggaa acattaacag attattgtta
      121 taagattgaa ttgaatcagg taagaaaaga aataacttgt gatatgaagc tctgttttga
      181 taaacaaatg tatgtaatca cagcagtagg agatgttgtc caaactaatt tgaatgaatt
      241 aggacatatc acgataaaat ttgatggatc tcatgagccg gaacttccag gaacaatgta
      301 tgtagaagat aaggaaatgc cgcatttaag aaaggggaca atcattaaaa tcgatcaaga
      361 ggatcagaat gggggaaagc tatatggaaa tcatgcttga tacagcaaat acaaaaagta
      421 tttattcttg tcaacagttt ttgaatttaa caggcgtaac aacaaatcct acaattctga
      481 aaaaagaagg agcgtcaaac ttcttctcgc atatgaaaga aatcaaaaaa ataatcggac
      541 agattcctat ccatattcag acaataggaa gaacggaaga agaaatgata gaggatgctc
      601 gagttatcat agaagaactg ggaaaagata cgtttatcaa ggtgccggtg aatcaagcag
      661 gccttcaagc gatcaaaaag ttgaaaaaag aaggttattt gattaccggt acagctattt
      721 acacagaatt acaagggtat ttagctatta ataatggagc agattatatt gcaccttatt
      781 ataacaggat gcaaaatcaa ggcattgatg ctatgaaagt gattgattcg ttgcggactg
      841 agatcgaacg tacgcaaaga ccaacaaaaa tattagcagc tagtttcaaa aattgtgaac
      901 aagtcactac agccattcga tctggagctc atgcgataac gttaggtaaa gagctactag
      961 atcaagtatt agagatgccc tcgattgata aagcagtgag gggcttccaa gaagattggg
     1021 tgcaaacttt ccaagcagaa acgattggtg aactaaaaaa ggtcgctgat tacttctgaa
     1081 tcaatttctc cataaaataa acgatagcct tctcgaaaac tcgaggaggc tatctgacga
     1141 ttcaatattc attaatttaa agtttgggtg actattacta actattactt atttctcatt
     1201 taaacgatgt attccctaac ctctaataca tccttgtaac tctgaagtta cgccagtttt
     1261 gaataaattt cataaaaaca aatgaaaagt gtcgcataaa aatatctttc gtgtttctct
     1321 agtataacag aataagaaga acaagtccag aagggatatt tttagtcttt tttaaaataa
     1381 cttttcagtc gagtttgagt ttatgatagt tgtttttgaa gcgatacggc aagtgaaaaa
     1441 atagggaagc atgaaagaat ctacttaatg gcgcattgat tcatgtgata gaaataaaaa
     1501 acttttttaa attatatcct catgattgta acaaatccag tgatttgttt tggtacaaat
     1561 aaaaaaaggc cccgatgaag ttgtggcaaa agtggtttgc ctcaaaaata agaaggaatt
     1621 tccaaaaatc acttctcaaa ttttgccaca tccccaattg ggtcaaattt gtttatttga
     1681 atatattttt tacataagga tctttaatag ttacattgtc ttttttcatc actttacgga
     1741 ttgttttatc catgaattcc gtatcaagag tttttgcttc ggtagcaatt ttttctagct
     1801 cagatttgta tttgtcttta ttagaacctt tatcctgttt ttttaccatt tttactagat
     1861 aatagctttg ctgataagtc gttggatctg ttacaggaat gatatcagaa acttccccgt
     1921 tttttagttt aaatgctgct ttttttactt cagtaggaat ctctggattg gttgaatcaa
     1981 aattcatttt tccatcagtt tcctttaatg ttttattttt tccataagct tgaacaagct
     2041 tggagaaatt ttctccttca tttgcttctt tttttgcttt atctgcttct tctttagtcg
     2101 aaaatagaat gatctgtgta ctaacttctg gatagaattc tttccaagca gtgtctagat
     2161 ctttatctgt taattttaca ttggctttca aaccgtattg gaaagccagc atctgtttaa
     2221 agagtttctt atatgtctct tctgtttggc caactgcttt taattgttcc ttgtagtttg
     2281 gaccgagttt tttgatttgt tcattatatt cttttgttac ttcctttgaa gaaatctctt
     2341 ttccgtatac atcatcaaat gctttgaaga caatcatttt ttttaatagt gtttgattat
     2401 ttgatttaac ttttgggtta tcataaaaat cctgaactgt aagtgttcca cctttaaacg
     2461 ttgcaatcgt ttcattggac ttgctacaac ctgagaatag taaaattgca catgctgaaa
     2521 ggcacataat tagtagttgt tttttcaaaa aatttcccat cctttcctta ttaaaaacag
     2581 tctaacagaa gaattaggga gacagtcttt cattttcgta aggttgctgt agatattctt
     2641 agttcgtaaa aatctattct ctctatgatg gagaacattt acatagtttt cacgagacaa
     2701 gaaagaaaag ggttgttaaa cttaatataa aggagggatg aaagatgatc aggattattc
     2761 ggttgattgg atctgtttta atagtcttag ttggttttgt tttaggaatt cttgtcaata
     2821 atgcacttgc ggatatgttt attggaaata gtgaatggat tggagcaatt gccggtacgg
     2881 tcggacattt agctgttttt ttactagctt tatttcttgc tagtaaggtc gaaggaaaaa
     2941 gaatagaaga ttatggtgtt tctgttcgag gaaaagactg ggggtattta gcgggaggtc
     3001 tcgttgttgg aattggagtg tttctgctta ttactagtcc attatatttg acgggggcgt
     3061 atcggttaga ttcaggaaac gcaaacattg tgccgctgat aacgagtttt attttattta
     3121 ttgcagtggg ggcatcagaa gagcttttat tccgtggctt ttttcagcat cagatgctta
     3181 gatttggtcc gttaatagca atgattggat ctgctgcatt atttgctctt ttgcacggat
     3241 tgaatcctaa catgacattt ctggcagttt tcaatatctt tttagcaggt tgctttttca
     3301 gcgcacttat ctacagtaca ggaagtttat ttacggctat tggtgctcat attacttgga
     3361 attggacaca aggagctatt ttgggtatac cagtcagcgg aacgcaagaa caaggttttt
     3421 tctccactct gatccaatcg caaaatacaa taattactgg gggaaacttt ggggctgaag
     3481 catctatatc aactacatta ttcttgttag tcttgattgt tggtttgtta ctctatgcat
     3541 ataaaacaaa gcggatgaag aaatacactg ctgtttatca ataaaagatg aagaaagcaa
     3601 ggctgatatc ggtcttgctt ttttaagtct gtcattgatt aaacgaaaaa gtaaacaaat
     3661 tcaagcgaat acacgacatc ttttgttaga ttttcacaac tgactgtcgt gttctcatca
     3721 ttttcatttt atataattcc tttatcaaat agaaggtaat tatatggaga gtgatagaaa
     3781 tgaagaaaac agcgaccgta ttatttgtta ttcttgggct gagtataatt gcagcaatga
     3841 ttggaatcag tacttctgca aatgaacggg caaatgagaa tctttcattt actgtgaaaa
     3901 ctgatcggat tgtttatgac atgacacaac aagtcattac tattccagtc aaaccaaaca
     3961 aaagcgtgaa tgctagtgat gtacatgctg ttctaacata tggttgggat ggtaatggaa
     4021 gttcagaaaa agttattggg gaagtttatt taaaagacgt acaatggacc gctggtattg
     4081 agtatacaat aatgatttct gcagaactat cgattgatga gatcaagagt aaagataaag
     4141 ttgatttgat tgttttttat gatggacaga tgacgatcac ggagaatttg aaaccaagca
     4201 gttggacagt tgttggaccg ccaagcagta cggatagcac aagtagtgaa agcagtacag
     4261 aaaacacgag cggtgaaagc agtacagaga gtacgagcag tgaaagtagc acagaaaaca
     4321 cgagcagcga aagcagtacg gagagtacga gtagtgaaag tagcacagga agcatgagta
     4381 gtgagagtag cacaggaagc acgagcagcg aaagcagcac aggaaaagta aataatgaac
     4441 ttgatttggt acctatcata ccaactaagg aacaaaagag aaatgcaggg agcaacctaa
     4501 aaagtgtgct aaaacagcct gattttgttt ctcgtggaat tgatcatcaa gaaaaagcga
     4561 aagaaacaga taatagaaaa agcgatttac cagaaactgg tagtagaacc ttgaacaagc
     4621 taattacatg gatgggtgtg ttactcattt tgattgtagg agcaagctat tttcgttctc
     4681 tcttccatcg agtgaaataa agaataaaag caacagatct aaaattgaat aaaacgatag
     4741 atttttgagt gaatatttca ttcgagagtg ctgtattgcc tggcgaagtt gtagctttgc
     4801 cagatctatt tttgtttact aacatatttg agaaacagga aataacagga acacaaatgg
     4861 aaaataagag caagaataat tgacaatgca cactgcgcat ggtagtatct ctgaaaaatc
     4921 agtaacgaat ctatttgtac gttattcatg tagctgtcgg aataagtcta aataatagat
     4981 aaaaaggagt atgtactttg tttgaagaat ttattgatat caatgaaagg caagtatatc
     5041 aatttttgaa ctattgttat gaaagagacg agaaattata tgtggtgaag gatatcgctt
     5101 tagatctaaa ctacaccctg gctaagatga attcggtgat tcaacaagca gaatcgtttt
     5161 gcgaaaggta tccagaatat aaattatctt ttctttcaga aaacaagatg ataaaagtcg
     5221 agttttcttc tcagttttta ctgagtaaag tttattctat acttttagag ggaacgattg
     5281 gttatatttt actggattca ctttacaaag gtacttatca atccttagaa aatcttagtc
     5341 aaaaaattat ataagtttgc gtacgattca aagaaaatta agagaactta atactatttt
     5401 gaaaaactat catttatctt gcagcttgaa aaaaggtaat ccgatagaag gagaagaata
     5461 ccgtatccga tacttttttc acttgatgta ttggcagtca ttcgacgaaa atcattctga
     5521 tatattatca agtataaaaa ataaacgtca actattagaa aaatttgcga aacatgcgcc
     5581 atatacgcgt agtatagata gaaagaaatg gatcagccta ctaagaattt cgttgcaacg
     5641 aatgaaacaa ggccactcga ttacgtatgt cccagaagag atgaacaaat ttgtgcatcc
     5701 attgataaca aaagaagagt ttacgcatta catactagag cctttttttg aagcaaactt
     5761 ctggtcgatg aatcacatca ctccaacaga gcaagcttac ctttatttta tgtttggtgt
     5821 aatgaacacc tatttggaag aagatttgtt ggatactcca gataaagtac aagaaagttg
     5881 gggctggatt gaatcgtcta ttgtagaaaa ttttgaaaaa aacttcaaga tttgttttac
     5941 aacttcagaa aaaaattatc tatatgtcaa tttggcaatg atccatttgt attcaagggt
     6001 ttttgggaca aaaaagaagg tggatgcttt tggaaaatca gcagaagaca gagattttca
     6061 gcatatattt ccagtgatgt atccaatcat gcaacagttt tttagtgggg tagctcgcag
     6121 aattccagag cttaatactt attatgaaaa gaacagacgg ttgatttttc aatactgtat
     6181 gttagtcagg gatattttta taaaacacga acaacctgtc atattctata ttcaatcaaa
     6241 gtatggtaaa acacaggaat tatgggcaaa gaaaaggata ttatctatga ctgaacgccc
     6301 aataaaatat agtgcatccg ctgatcagtt acctgatatt gtcattagcg attatcctat
     6361 cgacaaaaag gcatatgatc aaacgaatac acagatattt tactggaatg gacaacctac
     6421 aaaaaatgat tgggcgcgtc tgaaagctga aatcataaaa atcaggaatg agaaaaatag
     6481 atccatattg gaaaaattgg atttcgttaa ttaagaaaag cagattgttt atgccaaaat
     6541 taagacattg caaaaagatt attttcttta aaatcgacaa gatgaccaga aagcaagagt
     6601 tcgttgagct cttgctttct tttagtttta gaaaggaaca tcctgatttt atcttcgcta
     6661 aatccagtca aaagttttgc agattggtcg tagataatcg gttttttcaa taaagaaggg
     6721 gatgtacgaa tctcaatata tagttcactc aaactgagtt catctattct gtgaacaaga
     6781 tttttataac taatagaatg agtagagagc atttcggtca cgccgttgtt tgtcaatttt
     6841 agtaagtgga taaattcatt tttggttagt ggttgacgaa ttaagttctg ttcgataaac
     6901 ggtatatcat ggacgataag ccaacgtttt gcgcgtcttg aagacgagca gccagaacct
     6961 gtatacaatt taatcatcat acattcctcc aatagctaag tatttatgaa ataataacat
     7021 gaagaaatag tcgaaaagtg ccatttagag gtgtaaaata agaaaatgtt gtcacgtata
     7081 atggaactag ctagataaga aatactatta aatcaagaaa aaagaaagaa ggcacaagta
     7141 tgtcaaacac agccgacgca atagtagtga tcgatttaca aaaaggggta tgttttgatt
     7201 cttcgccgat acacgaatta gaaacagtga cagcattagt gaataaaaga atcaaaacat
     7261 acgaaacgat tgggaaacca gttattttcg ttcaacacga agatgctcaa ttagtgccac
     7321 aaacaaaacc atgggagcta ttgtcggaat tatctattcc tgagcacagt tattttgtga
     7381 gaaaaaccca tgcgaattca ttttataaga ctaatttgag tgttttatta actgaattgg
     7441 actgtcattc gatcgaatta tgtggggcac aagcagagta ttgtatagat acgactgtga
     7501 agtttgctca tggattagga taccagctta tgatgtgtag aggagcaacg acaacttatg
     7561 ataatccttt tatgaaagca aaggaaacaa tcgcttttta cgagaaaatt tgggataaac
     7621 gatttctgac atttttggag caataagttg tttcttcgtt taaaaaaagc agaatggata
     7681 ctggtttatc cattctgctt tattacgtaa aatgagaaaa taatcctgtt ctatttttat
     7741 gggataaacc atttattttt tgtataaaaa atgtttgcgt ttacatataa ttctcctaga
     7801 aacataaaaa ggagtgaata gcttgagaac aaaacaagta ttaaagggag cgttggtcat
     7861 cttatttgct gtaagtacaa gcgtcccgca atggacagta tatggagaga gcatttcttc
     7921 cgaatcggaa tctcgggagg tagaaaccac agaaactact gatgctggaa atgaaacgaa
     7981 atcgagtact tcacaaacag aaacaaatac tgaaacaacg gttgagacat ctgttactag
     8041 cacagatcaa gaaacgaccg ctaccacaga taaccaacaa acagaagaag caacagctac
     8101 aaaaatgtca aacgacgaat cctcttctgc taaagtaagt gacaaagaga atgggacaga
     8161 aaatgatgat atcggtaaag gtgaaaattt caatggtaaa gaatatttga attacttcgg
     8221 taaacagatg acagaaatac ttaatggacc aacagaagac atcaagtcaa aattaataga
     8281 gttattagtc actgagcttg ctgttaatga ttatggtgta gataaagaga ttatcaaaca
     8341 tctgattgct acttatctat tgtacgatat cgcttttgca aaaaacagtg tgttgatgtc
     8401 gatgcttgga ggtacagatg atgggacaac gcctacagaa aacacaggct tctatccaaa
     8461 tacaaaacaa cttcatcaaa ctgtactaaa caaacaagat ggcaaaatga tggatgtata
     8521 tgcatattat gtcgatcaag gttcagataa aacagtcatg atccatggcg gatttcgagg
     8581 gaattggaat aacgggatcg ttacagaaga atacaatgat ttttataagg ccgggtataa
     8641 tttattattt gttgattcca gagcgactgg taatagcgga ggcgattatg tgacctatgg
     8701 acaatatgag tcagatgacg tcctttactg gataaatcaa gaagttcgag agcggccatc
     8761 tcaaaagatt ttactttatg gtggttcgat gggagcagct acgatgatgt ctgtattagc
     8821 gaaagatatt cctgtcaatg tcaaaggaat tattgaaaat tgcggatttg catcaattga
     8881 tgaacaacta cgttttacgt acagccagac agtagccccc gcactaccgg atgccataaa
     8941 aaatcagtta gacatcattg gggatcaaga gcatgaagat ttatttatgg gccttttgaa
     9001 acaatactat tttgatcagg agatgcattt ggatacaaaa gctgctctac ctacgattgg
     9061 aatgtcagat tcattgccta aattgattat ccatggaaca gcagatgatg tagtacctgt
     9121 gtctaacgcg caaaaattat atgagttatc aggagggtat aaagatcttt tattagtaga
     9181 aggagcaggg catggaaagg cacaggaagt tgaccatgct gcctacacga aacatgtaac
     9241 ggactttttg aaagtagtgt tccatgatca gataaacgta aagtatgtgg atgaaaacaa
     9301 taaaagtcta ctcaaagacc aggacgaaat ccagctttac ggagcttatg gagaaaatta
     9361 tatgacagag caaaagacat tcgaaggata cgaattagca aatgttgaag gaccgactga
     9421 agggattttt aatgagacga taccaactat tattttctat tacaaaaaaa tcccagtagc
     9481 tccaccaaaa aagcaagatc cgacagagaa tgcagacaat aagggaaaga atgaacctcg
     9541 agaggatcaa tcaagtgatg atacgcaacc gattcagatg acagtgaaag tacaaaaaaa
     9601 gacagatgca aaaaaaagaa tgttgccgaa aactggagaa aagaaacatt cgcttctcat
     9661 ttattcaggt ggatttttat ctgttagcac gattatcaca tggatttggc gaagaaagag
     9721 agggtaaatg aatgaaatgg ttcaagcgaa tagtgattgc agtaccagtt atcatcgtag
     9781 ttattttagt cactgtattt ttgattaatc aaatcacacc aaagccgatt agcttgcttg
     9841 ttagaaaaca atttgacaca agtgaaaaag aacaagtata tgcccgacca gatgatttcc
     9901 agcaaaaaat cgaacaaatc gatataaaaa aagaaatatc ctatccttca aagtacaaaa
     9961 ataataatat ggatatctac acaccgaaag tggaaaagaa aaaattaccg attcttttct
    10021 ggatgcatgg aggcgcatat gtaggagggg ataaaaatga ttgtcgtgat tatttggaat
    10081 atctatgttc agacacgcag caaatcatcg taaatataga ttatgaacga tcaccggaag
    10141 caaaacatcc tacaccagtt atccaattga acgaagccat tcaagcagct aaaaaagaga
    10201 taaaagatca agcagattgg tcgaatattt cgattggtgg aaattcggca ggtgcacaaa
    10261 ttagtggaga atatttgtta gcacttcaaa atgaagaaat taaaaaagct gatcatcttg
    10321 atccgacttt ggagaacaag cagattacga agtttatttc tttgtctggt ctgttggatc
    10381 cttctgcact tactcaagta agtgataaaa tctctagctt tctttatgaa aaatgtggat
    10441 gggcttactt tgataataag gactttgaac aatcagagaa ggtcaaaagt ttagccttgg
    10501 tcagacatgc ggatcgttgg acgcaaaaca cattcttaac ggatggaaat acaaatacgt
    10561 ttacgaaaca aatggaagaa gtaacttctg tatttgagaa agcgggggtg aaagtcacaa
    10621 aagtagatta tgcggagaaa aatgctgttt tgaaccatga atatcagttt gatttcagca
    10681 ataaacaagc gcaagaaaca tatcaaaagt tagtaatgtt tttcaaggag taaagtctta
    10741 tgatgcaaaa gaggttgtaa tctgtcactt agctccaagt cacaaggagc aatttcaata
    10801 aacaagcttt tcatggttta tgaatattgt aacttgtgac tgaggagtta gagatggcaa
    10861 ggggcacctt ttattcgaaa tttgggaata tcacacgatt attgtcacga tctcttttac
    10921 gtaaatttta taagtcagat ttgttgtttt gatcgtttaa aaaaagcaac aaatccgaag
    10981 cctgcggagg caagaaggga caaaaagccg agtggtaagc aaaagaatgc tggttccact
    11041 aatattccgt tgttattgat ataaggctgg gaacaaagat gaatagcaaa taaaatcaat
    11101 gcactagcga aaaaaagcaa agaaatatgg atagctttca taagcagcac ctcctttttt
    11161 ataattgtat gataaataga ttaagaatct ataaatgtga caagtttgtt aggatgtgaa
    11221 taaaatgatc atctagcgaa cttgaaacaa agatggctaa tgataagcaa ttaatgattc
    11281 agcaatcaga taggtacaaa gtttaccatc atacgtctag accaataata gaaaatatgg
    11341 gaatataata gaaaagattt aggatacccc caaaagaatt tccccaaaaa ttcgatgtat
    11401 cctaaacttt tttattttac ataacaatca tatcaactgt gaaataaaat tacaaatgat
    11461 aacctaaaat gaggccaaaa gccagtgtta tcaaggaaat acttaattca ccaaaatttt
    11521 ttttaatttt ttagaaaata agaaacaaaa ataataaaaa aaagaaaaga taaatttttt
    11581 actactgtat actaactttg ttatcgcatc taacaagtaa ctcgaataat accaatacaa
    11641 ccttctccaa agatgcgatg acatacgttc taacccaccg actattgatg ttctgtacca
    11701 cccttgcaga accaaaaaag ccactaccaa atatttggta gtggcttttt ttgttttatg
    11761 gatttttcgc tttttctgtt atggtgctat tctttgattt cagaaatagt atgtcgaact
    11821 ttagcgatag aacgacgtga ttctttttcg ttgtaaaaca tcaaaatcac atatagagag
    11881 tccaaaatgc ttagttgaga aatacgggaa gctaaagctt cagatcggaa ttctgtttct
    11941 tcagaaatag aaataaaaac gacatcgcca agtttagcaa gggaagaatt agcttggcta
    12001 gtaataacga ttgtttttgc tccagttttt cttacggttt cagcaatatg gattgtttct
    12061 tttgattgcc ctgtgtgaga aatcaaaaca gcacagtctt tttccgtcaa taaagaagct
    12121 tccatcaatt gaatatggta atccgtgctg tgagaagttg cgatagggga acgcaaaaat
    12181 ttatgataag cgtcagttgc tacaatctct gaaccgccaa caccgaaaaa atagacacgt
    12241 ttagattcat tgataatagt agccgctttt tttagatcct tttgttgtaa caactttttt
    12301 gtgtcattta aagttgaaat actagactcg aacacctttt gcgccatagt gagctcagta
    12361 tctgttttct gaatattttc gtggatagaa atagcagata gatcattttc ttggatcaac
    12421 atggccattt taaaatcttt aaatccatta taaccaagtt tttttgtaaa ttgaaaaaaa
    12481 gtagagtcag caatgcccag ttcacttgct aaattgctaa taggactatg acttactttg
    12541 cttggatttt ctaaaatata atcggcgata gcctgttctt ttgggctgaa gtcgttgtac
    12601 attgttttga tttgtagtct tatggagacg ctcatgagaa ttcctcattt tcataataag
    12661 attatcattt tttccgttct cattaatgtt aaagatacaa ggagagtagg aaaaaatcaa
    12721 acaaaaaata tttttgtaaa atctatttac aaaaaaattt ttttgtataa tgagggtata
    12781 agaaatggag gcgctatcat ggaaattggt gtaattggtt tgggaaaaat gggattgaat
    12841 ttagctttaa acttaaaaga gcaccatcac aaagtacaag gactcgacat ttcaagtaca
    12901 gctgttagtc aggcaagaga aaatgacatt cctgcttatc aagagatctc tgaatttcta
    12961 gctacttttg agcagaaaaa aatcatctgg ttaatgcttc ctgctggaga gataaccgaa
    13021 aacatgttaa gaaaactttt tccaaaactc ggaccaggag acattattat tgaaggcgga
    13081 aactcttatt ataaagattc aatccgtcga gcagaagagt ttcagaaaaa tgaaatcggt
    13141 tacttggatt gtgggacttc tggaggaatc gaaggagcac gacatcaagc ctgcttaatg
    13201 attggaggag ataaagaaac ttttctatcc gtggaacaat tattcaaaga tgtagcaatt
    13261 gaaagaggat atctgtatgc tggtgcatcc ggaagcggac acttcttgaa aatgatccat
    13321 aatggaatcg aatatggaat gatgcaagca atcggtgaag gattccaact tgtacaaaaa
    13381 agtgagtatg cttttgactt ggaacaagtc gcacgtgttt ggaatcatgg ttcggtgatt
    13441 cgcggctggc tgatggaaat catggaagat cagttaaacg aacatcccaa tttaagtaat
    13501 tatcggggaa tcgtttctgc atcaggtgaa gcaaaatgga cggttgaaac agcgctagaa
    13561 atggctgttc cagttccagt aattgcaact agtttgttca tgaggaatct ttcacaggaa
    13621 gaagatagtt tttcagcaaa agtagtttca gcactaagaa atgggtttgg cggacatgaa
    13681 atagttgaaa agggtgagta aaatgccaga ttatcatata gcaatcgtta attccagcag
    13741 tttcggaaga gtctttccac accacttgga aagattaaag tcagttggag acgttcattt
    13801 ctttactgtt gatcaagaga ttccaggaaa agaactagcg gagttactta aaggattcaa
    13861 tatcattatt gcaagtgtta cgccattttt tcgacgagat ttttttgaaa acaaagatga
    13921 actgctattg atttcccgac atggtattgg ttacaacaat attgatttaa aagcagcaaa
    13981 agaacaccag acaatcgtat ctatcatacc tgctttagtt gaacgaaatg ctgtagcaga
    14041 aaataatata actaatttgt tgtccatgct tcgacgcacg acacaagcac aagcaagagt
    14101 caaggacgat cgatgggaag atcgggcgga gttcgtcggt aggacgctgt ttaacaaaac
    14161 agttggtgtg attggtgcag gaaataccgg aagcggagta atcgaaattt tgcgtaacgg
    14221 ttttcgctgt gatgttctag cttatgatcc ttacaaatca gatttataca tcgaaagctt
    14281 tggtgcaaaa aaagtagaac ttcaagaatt actggaacaa tcagatatca tttgtttatg
    14341 tgcgaatttg acagaagaga actatcacat gatttctgat aaagaagtcg atcagatgaa
    14401 agaaaatgtt tatctatcta atagtgcaag aggcgctcta gttgatgaag aagcgatgat
    14461 taatggtctg aaaagcggaa agatcgcagg atatgccaca gatgttttag aagaagaacc
    14521 gggacgtcaa gatcatccgt atttagcgtt tgaacaagta gtgattacgc cacatatatc
    14581 ggcctacaca agggaatgtt tagaagcaat gggtgaaaaa tgtgtcagag atgtcgaaca
    14641 gatcagtcag caccttcttc ctgaacgttc tgtccagaca aagagcaact atataaaagg
    14701 atgatgaaga aaatggtgaa cggtttaaaa gtgaaaacag gccctcaatt ttatttatat
    14761 gaagaaggtg gtatttcaaa agtttctgat ctcttgaaat catacggcgc gaaaagagta
    14821 ctggtcaccc acggaacggt atcatgggaa aaggcgctac ctaaattagt atttttgaat
    14881 gacgaaacga ttcaattttt ttatcatcgt tattctggag aatgtagtta tgctgaagcc
    14941 agacgaatag ctacaatcat caaaaaaatg aaatcgattt cgtagttggc gttggcggag
    15001 gaaaactgac cgatctagtg ggttatagtg ccaatcttgc aagaatcgat ttcggagtca
    15061 tccctacttt agctagtaat tgtgctccat ggacacctct tgctgtcatg tataaggaga
    15121 atggacaagc agaagggaaa acagaacact tttacagaca agctgctttt tttataacag
    15181 atccccaatt agtgattgat tcccctgttc catatttcat tgccgggtta gcagatacac
    15241 tagcaaaatg gtatgaatct gagctcatct tagaacaaga ttttttgcaa gatgaatcat
    15301 ttttgaagct agcacaagat actgcgaaga tctgcaaaga agagattttg aatcattctg
    15361 ctaaagcaat ccaagatatg gcacaaagaa aactgacatc ggagttcaaa cagctttcag
    15421 agattatctt tgctgttgct ggtttagttg gaggattagg agataaatac gcaagaaatg
    15481 cggcagcaca tgctatgcat gacgctatca gtaaatatat tccggaaagc catcgttttc
    15541 tacatggaga aaaagtagcg tatgggattt tctatcaact ggcactagaa ggtcgctggg
    15601 aaaaaataga cgagttatat cctttgtatc gggaattaga actgccggtt tctctagaac
    15661 aaatgggatt atttccgaaa gataaacaga taatagataa aatggctgaa tttattgatt
    15721 cgaaagagaa agtgcatttg attcctatag aggtaacaca gaaatcactg atacagtcaa
    15781 tgatggaact ggaaggttat atcagaaaga tctgaaggag gaggacacat ggaaacatta
    15841 agtgtagtac aaagtttgtt gattgcttta tgggtagccg cggttatgtc ccgctggtta
    15901 ggtggaggag ccacgttgac cttacgtttt tctcctttga tgactggatt agtcgtcggg
    15961 ttgatcatgg gggaagttgc acaagcgatg attgttacag ccgctttgca aatgatttat
    16021 atgggggttt tttctccagg aggatcaatg ccagctgaac catctattgc agcagctatc
    16081 gcagtaccag tcgcattatt aggggatctg tcgccagaag cagcgattgc cgttgcggtg
    16141 ccagtcggat tgctcggaag ttacttgtat cagtttcgtt ttttcatcaa tactttttta
    16201 ggtaaatata cagacaaagc tgtagaagat ttgaatacaa aagctatggc ccgatcaatt
    16261 attctttatc caacggttgc ctcattttta ttattcgttc cgcttgtttt cattgcttta
    16321 tatttagggg cgccattgat cgctgatgta atcaaagctt tagagggaac agtcgttatc
    16381 cacattcttg aagtagtagg tggagggtta gcggcaatcg gtatcgcgac aactgtatac
    16441 gttatcggtc gaaaagatta tctggtcttt ttcttccttg cttattttat gagtgttgtt
    16501 ttgaaatctt tagatattac gatggttact tatgccattt ttgggattat tatcgctttg
    16561 atctttgtcc agtcgcaaaa agggaaacaa gtacagcctg ccacaagcaa taacaattca
    16621 gcatcttttg atgacgaaga tgactatgat gacggatttt aatttaggag gaatgggaaa
    16681 tggctgaaat aatggataat caaacaggtg ctcctgaaga aatcacgaaa aaagatgtaa
    16741 caaaagccta tctgagatgg cattttgcaa acgaaattcc tcactctttt gaacgttact
    16801 tggcgccatc actactatac ggaatgatgc caatcttgaa aaaactttat aaagatgaag
    16861 actctttaaa agcagcatat aaacggcagt tactgttctt caacactcaa ttaagctggg
    16921 gtggcggtgt gattactggc ctaatgtctt ctatggaaca gcaaagagca gaagaagagt
    16981 atgaagaaaa agagatcttg atgcaggatg acttgatgta taacacgaaa gcgggattga
    17041 tgggggcact agcaggaatc ggagatgcaa tcgattcagg aaccgtccaa tatattttta
    17101 tcgcaatcgc ggtaccttgg gcacaacagg gaagtccttt aggcgcactt tttcctttta
    17161 tctgctttgg gttgtatcag gttttactag gggtattttt cgcaagacaa gctttcacta
    17221 tgggccgtaa tgctacaggc ctaatgcaaa acactggtgt tcagacagcg attgaaatgc
    17281 tatcagtttt agggttgttt atgatggggg tgcttgcggg aaactatgta aaagtagaat
    17341 cttcattgaa atttgcgata tccggacgtg agtttgttat ccaggaaatt cttgatcaaa
    17401 tcgtgccagg gatattacct ttagctgtcg taatgggtgt gtacttgttc tatacgaaaa
    17461 aaggattgaa agtcacacaa gcattattat ggctgactgg aatattaatt gtgctagcag
    17521 gaatcggtat tttataaaaa gaagggaacg tgaataagat gggtgtagtc aatttagcgc
    17581 gtgtggatga gcgtttgatc catgggcaag taatggtaac gttatctcaa aaaagtggag
    17641 tgaattccat ttttgttgta gacgaagttg tagcaaaaga taaatttatg agagatcttt
    17701 acaaaaatgc aggaagccgt acaggacaaa aaacaattgt cattacacca gagaaagcta
    17761 aattctattg ggatgaatat caatttaaag aatacaattg tattttgatc gcaaaaacag
    17821 tccaaacgat ttacgactta gtaaaacacg gaatccccat ggaagaattg aatattggag
    17881 ggattgctca aaaaaatcct gatacagatc ttttagtgac gaaatctgtt tatttaaata
    17941 aatcagatgc tgaaaaattg aaagaaatga atgaacaata tggcgtaaag aatatttatt
    18001 ttcaagccac tccatctgca cctaaaacag gattaaaaga tgtattgaac aagtttgatt
    18061 tgtcataaac aaatgcttag aaaaaagata aagagggcga aaaaatgaag aagaacgagc
    18121 tgtttcgtga ttgggaattt cgttatcgct atatttaccg taagcgccgt acaaaaaaga
    18181 gtaaacagcg ttttctttca gcattagtat cagatattta cagtatgaga acagatgtga
    18241 cagtcattgc ttacgatacg ctagcctacc gatcaaaaaa tatttatgtt ggagatatcg
    18301 aaaaagcaga gaaagttatc tgtacttatt acgatacacc cgttcatgca ttaggaagtt
    18361 atttcatgtt tgattggaaa gaccaaagga aaaaaacaat ctactctata ttattgtcct
    18421 tcattttgtt gttcagttta ggttggtggg gaatgatgat atataacaaa aatcctcatc
    18481 acgtattcga tctgctatcc gtataaacag gtatcactgt actagcattt gggagctact
    18541 ttttcttatt gggaaaagca gctcgcggat ggcctagcag acaaactttt attcgaaata
    18601 cttcatccat attaacgatg ctagaaatga tccgtacgat tgatgatccg agtgtagcgt
    18661 atgcgtttgt tgatgaaggc tgttatggag aaaagggctt agacagcgtg agatcgggta
    18721 tgaaaaaaga ggctattctt ttttatctag actctgttgg ggcagataca ccgctacagt
    18781 tttcgggaaa ttatttttca aataaagaac agtggctgaa acaagttgat aagttgaaag
    18841 aaaaaaacgt caattatatt tttagtgcaa gaaaaaagca agcgcagttc ttctatttga
    18901 ccaaaacaga tttacgaggg aaaacattta attggcaaaa tgccaatcag attattgctc
    18961 tttttcgata agtaaaaagt gcaaaacaag gagtgagaag actaatgttg gaaatcatga
    19021 tcgttacaca tggtaagttg agcgaaggac tgaaagatgc ggcggaagta attgtcggga
    19081 catcgagcca tattgaaaca atgtgcttag agtctgggga ggacatccaa acattcggag
    19141 agaaggtcaa agaaaaatta actatgatca acacagcaga acccatgcta gtatttgttg
    19201 atctaattag cgcaagtccg tacaatcaag cgctcttggc agtcaatgaa ctgactgaag
    19261 aaaagaaagc agccacttat gtcatagggg gcgtgaatct gccaatgttg ttagaagcaa
    19321 tcaatcatca gatgcttcac acaccgatag aagaagcggt ggaacaaatt gtcaaacaag
    19381 gaaaagataa tttaactgtt tggcatgctt cagcggaaga aaaaaaatta gatgaggacg
    19441 atttttagaa ggaaggctga aaagatgaag tggggattta gatggtatgg tgaaaaaggg
    19501 gattcgatcc cattaaaata tgttcgccaa ataccgggaa taaatgggat aattggaact
    19561 ttgctgacta aaatgcctgg agatgtttgg gagatttcag aaattgagga gttgaaagca
    19621 tctgttgaga gccaaggact ggaactgctt ggaattgaaa gtgtagcgat ccacgatgca
    19681 atcaaggcag ggacagatga acgagattac tatattgata actatatacg aacaatcaaa
    19741 aacttgtctc gatgtggggt tcgattgatt tgttatagct tcaaaccaat ttttggatgg
    19801 gcgaaaactg atttgtttaa tgaaaacaac gatggaagct attctctttt atacgatcag
    19861 aaaatcgtcg atcagatgga gccaggagaa atgtatcaat tgatacacag ccagtcaaag
    19921 ggattcaaat tgccgggttg ggaagaagaa cggttgaaaa aattcgctca gctggaaaaa
    19981 ctgtatgaag gaatgaccga agaagaatta tttgaaaatc tgacttattt tttagaaaaa
    20041 gtgattccag tgtgcgaaaa agcagatgta aaaatggcta tccatccaga tgatccgcca
    20101 tgggaacttt ttggttttcc tagagtgacg aaaaatcttg gcgacttgaa gaaaattcta
    20161 aatgcagtag attctccata taatggacta acgctgtgca caggatcttt aggggcaaat
    20221 ccggaaaacg atttagttga aatcatccat gaggtaggtt cgcgcatcca ttttgtccac
    20281 ttcagaaacg tggagttttt aggagaaaga aagttcaaag aaacggcaca tccctctaca
    20341 gaaggctccc ttgatatgta tcatattatg aaagcgttgg tgcaagaagg gttcgacggt
    20401 cctatacgcc ctgatcatgg tagaaccgtg tggggtgaag tggctatgcc tggatatggc
    20461 ctgtatgatc gtgcaatggg actcacttac atgcaaggtc tatatgaggc gataagtaag
    20521 gaaaaaccac tgggagagca ttatgaataa aatcaagctc ttcatctttg atatggatgg
    20581 ccttttatta gaaactggac gtttagctta tcgtgcttat gtaaaatcag cacaaaagta
    20641 tgattatgaa atgcgtaagg aagtctatta tttactaact ggccaaacag aaatggcgat
    20701 aagagaacaa atgggcattt tgtacggaga agatgttcca tatatcaaat ggcgagaagc
    20761 aatcaatcaa tataaagaaa aaatcgtgaa agaagataag agagtatata cgaaaaaagg
    20821 agcagaagaa attttatctt ttgcaaaaga aagaggaatc catacaatcg tggcttcgtc
    20881 taatacacgg gtaaaagtag aaatgtattt aaaaatggaa aatctatatg ccttgtttga
    20941 tcaaatcatt tcaggggatg atgtaaaaaa aggaaagccg gaaccagaga tctttttgaa
    21001 agcttgttca aaaatgaata ttcctccaag tgaagcactt gtatttgaag actcgattgc
    21061 agggatcgaa gcagcaaggc gagcgggcat tttgtctttt ctcatcaaag atcatttgat
    21121 agggttgccc gatcataatg ggaagcataa gttgaaagta gatgtaagtt tatatgaatc
    21181 caataaagca gactatgaat tttatgacct gcatcaagca aaacgatttt tagaaagtaa
    21241 agggttgtat ctctaataaa gaggactatt ttctagtcct ctttatttat tgtcctgcac
    21301 gactgaggga acagatgaaa aagaagtaat tttttatttt gaagtatcta ccttatttgt
    21361 agtgaacaat ttagaacaaa cacaaaacgc atctactgtt atctaccagt agatgcgttt
    21421 aaagagaaga acgagaatcg tccatatgag ggtttccact ccttatgcag atttttttaa
    21481 ttcttgcact gcttgttcga tcgtaacacc tgtcgctaca tgattggtat tttctttgtc
    21541 aaaaacgaca aatagattca aatctgtatc caaggttact cgatattgta tatctttgtt
    21601 atagaatgtc atagccattc tcctttcttt aaatacttgc tgaaaggcaa cgaacgcgtt
    21661 gataacgaaa ttccatttat aagtatacca ccaattgaaa ggttttcata gttaagaaat
    21721 tttaaagtca aaagttgcag ataaaagcaa ataaacaaga aaacaaaaag tgaatcaatc
    21781 gcggttattc aactttttct tcgattcgaa gacaatagaa gacaatgaat tttatatttc
    21841 attgcattat ccttgtgtta ttagaaaaag catgatatat taaatgagta atctattttg
    21901 aaacgtaatt tgagcgatat atctacatag ataaaactcc ttttgcctat caaatattta
    21961 ttgcaacgaa aaaacgtaca ttgatacgta tcaggaggaa tttatttatg aataacggta
    22021 cagtaaaatg gttcaacgca gacaaaggtt ttggctttat cactggtgaa gatggaaatg
    22081 atgtatttgc acatttttca gctatccaag gtgacggctt caaaacttta gaagaaggcc
    22141 aagcagtgtc ttacgatgtt gaagatggtc aacgtggccc tcaagcaaca aacattgtta
    22201 aagcttaata aaaattgact tttaaccgtc ttttcgaaag aaaagacggt ttttttgtac
    22261 gaaaaaattc attggtcgtt gaatgggctg aatagtagtg tataaaacaa ttttgtggag
    22321 cgtgaagctt ttatggtaga ggaaaatacg aataaagtga tgttgataaa aagattccta
    22381 tcttgaatat gaatcgagat gtcttgaaaa tgaatgagtt tgaccaatca ttatcctatc
    22441 gaaatttaca gtttcgttcg attggtctgt caattaatga aaaagtaaaa aagaatatgt
    22501 tctaggaaga gggcggcaca tctaaatttt taagtaacgg acagttcaaa tatcaagcat
    22561 tgaaagaggc tatcctactg tttgaaggaa tacgaatata taaaaacgac aaagaggaaa
    22621 aagtagttca ttaaatccct tcaagtgatg aaagaatgag atctaataaa acctgatctt
    22681 tcatctgttt ttttaataac gtaataattt ttttattttt tgtgctttcg ccgttattgt
    22741 agagtgattt agcattctta tttttaaaaa taactgtgtt atatattaaa aaaggcataa
    22801 tagttataag cttttttaaa gaatatttag aaattattaa ttttcgtctg aaaacaaaaa
    22861 aagaaagcgt tattatttta gtctattttc agggggggaa ccttatgata aaaaaactat
    22921 ttttactatc aatattaacg ctagcaatgg caggttgttc tgactctggc ttagcaactg
    22981 aactgatcac aaaatcaact gaagccacga cagaggagtt aaaatgtgga aacgatcaca
    23041 gtgactacag aggcttaaat cgacgatgca tgggaatatt aggcagaagc aattgtaagc
    23101 atcgatgtag taatcgaaga aggtgtagaa gaagcaacag aaaaatcaaa agaaagatca
    23161 aaagaagcaa tctgtgctgt gcatcgtata cagacgtcga agatgcggta cttttaaacc
    23221 acgtcaggag atgttcattt atgtctaggg aagttggcga tgaaccaaaa ctagagtacg
    23281 cttttagttt tgcacgtgag gatgaagata accctaaaac ttacgacatt ttagcttgat
    23341 ctaagatggt taaagctttt taatgaagta agaggagata gaaggctttt ctagttatat
    23401 taggtaagat aaattccttt ttgctccaaa tttgctaaac attctgctag ataaacttca
    23461 tcgtgttccg gataaatcgg cacgttgcgc tctacagtag gtaaatcaaa aaatgcttcg
    23521 catgatttgc cacgatacat aggatttttc cactctggtg ctgggctgta accacgtttt
    23581 tccatttcat ccatgacaag ctgatgatat tggaataatc tataaggaga atagtgaaaa
    23641 acataatcca cagtagcatg tttctttccc cagccatttc ctcttaatgc acagcattcc
    23701 cgatgttgtc ctaaaagctg ttgacgagga agctttgaaa tcaatttttc atgccataat
    23761 cgcatagaaa tgtctccttt atatatagat tgttaacctt agacgttaaa tgaaaaaacc
    23821 agcaccaagg ctggaaaaaa tcacttcagt ttgcttgctg tttctgagcg accaattagg
    23881 taatcaacgg aaatatcgta aaagtcagcg atatagatta attcaatgat gcttggaatt
    23941 tcttctcctt cttcgtactt acgatatttg aataaaggaa tacttaacgc gctagaaaca
    24001 gctacttgag ataaagatcg gccagttctt aagaaattca gacgacaacg taaagtatcc
    24061 agtaaaatag cttcttcgat actcatttaa tcacctttca ttatagtaga atagttataa
    24121 tgactataga aagctacaca tgcaatcaat agtactcaat gttttttatt taaacgtatc
    24181 aaataaagaa tgacgatcaa taaagacaaa accagtcctg ataattcttc tataaaaaca
    24241 ctatcgggtt caaagtagtt gtaattaaaa actttccaca taaagtagat agccggaaga
    24301 gtcaaaatag ctaaataaaa ccgttctttg ttccaattca taagataaac aatacttgcg
    24361 accagtgcga ccgcaggcac ccaaaacaaa actctcacca acgtaatcat gtaacggact
    24421 cctcaacgat tcatataata aaagataaca tgttattgca tacatcacaa atgaaatgct
    24481 aggaaaataa aagaagaata gatcaatgaa aaatgaaaaa gcagaacagt tttctgctga
    24541 taagcaggaa ctgttccgct gaatgtgtct tatctatcga gaaaaaatca atggattgat
    24601 atctaagtaa ttcaatacat ataaaaagag gacgatcgca ttcactaaaa aggaaagaat
    24661 gatgatccca tagcgaatgg aaacatattt aacaaatact tttgtttgaa tacgggcata
    24721 tctttttcct ttatagctga gcatcatgac aagaagcaaa agggtaatca caccttgaaa
    24781 aatcattcca ataaacagaa ttcctgaggt ttgataattt aattggagaa aataagctgc
    24841 tgccgcaact aaatcgaata aaatcgcaat aaatggcaaa ataatcactc ctaacattga
    24901 gaataaataa aagaataaca ttttttctaa aataagcaaa taaaaattaa agtaacaaaa
    24961 tagttatgac acaagtaaaa gaaataagaa taacaatatt attttactta tattttgaaa
    25021 cttgtgagag tttgtcattc gtgctatagt tgaaaagtaa caaaaataaa ttttatttat
    25081 gatgtacctt tagctcagtt ggttagagca gacggctcat aaccgtccgg tcgtaggttc
    25141 gagtcctaca aggtacataa gaggttgtga aaaagctgcc ctgcatcaag taataagaac
    25201 agccaagtaa aaacagcttc ttatgttttt acttggctgt tcttattact gcagatgcaa
    25261 gcttttgaat accgtttaag gaagaggttg tgaaatcagc gttttgacct gagtattagc
    25321 ttaacaaacg gaaaaatagt tcctcatatt ttgccgtttg ttaagctaat accgagggtc
    25381 agctgattga acaccattta agaaagaggt cgtgagcctg ccgtttcact ctgtgtcata
    25441 aaaaagccaa tctactttga tagaaaaagc acgtaggact aaaacagttc tgcgtgcttt
    25501 ttttattgtg tgttaaatat cgtaaatgaa gatagatatt tttatgattc gacgatagag
    25561 acgttgaaat ctttcaacag ctctaaagcg gattctccgt gttttctact ggagactaac
    25621 attggatcaa caaaaaagtc tgcttcagga aaagcactct gcaggcctat cagattagag
    25681 agtacacaca agtttgtttc aatcccgact acttcgatct ctttttgttc ttctttgctt
    25741 tcaaataaag tttcctggat ttcagctaaa gtttcaggtg gaatcgtaaa gtaatacttt
    25801 ttaatcactc gttcattttc ttgtggagaa agaagaggga tcaattgcaa atcttctcgt
    25861 tcttcttctt tgtccttcag ttcaactggt atatcccgtg tgaagagaat atattcatta
    25921 ttttggcgtg ctttagctaa tctttgagca atacgttctg ccaattgatc tgactctgga
    25981 atataaaatt cgctgtttgg atcaagaata tgattttgca tatcaataat aactaacatt
    26041 ttctttcctc ctacattcat atcgtcattt tattcttttt aatagaagtt ttttaaaata
    26101 taagcaacac cagcttcttc attcgtcagt gttacttggt ttgcaatttc ttttacttgt
    26161 cgctgtgcat tccccatcgc aatactcaac ccgctgtaat tcaacatagc aatgtcgttt
    26221 tcttgatcgc caaaagccat gatgtcactc gattcgattc caaggtaatc cgccagaaaa
    26281 acaagtgaat gagctttagt gatacctgta ggcgtgattt ctaagaaatg cggatcagag
    26341 aatactgatt ggataccatc tgcttgcaga tctctttgta gcttctgtag tttttcttca
    26401 ctcttatctg ttaatgctaa tttaagatag ctaagagaag gatcttccaa catatttaaa
    26461 atttgatcaa aagaatggtt ctgtaaattg aaatgttcat aataagggtc atctttgact
    26521 agatttgatt catcgaatgt cagctgatca tcttgggttt ctcggctagc aatgatggat
    26581 attttcgctt cttgagcata gaagaaggct tttctcagtg tatcgctatc tatttttgaa
    26641 cgtctgagaa ctttcgccat ttttccatcc ttcatttcag caatcagtga accattggaa
    26701 gaaatcaaat agccaccgat tcctaattcg gaaaaaatat ctttagcaga aatgctgttc
    26761 cttccggtag cgacggttac gattccgcca ttttcatcaa atttccgaag tgcttggata
    26821 ttttcttctg gtatctttcc atttcgatca agcaaggtac cgtctaaatc aatcgctgct
    26881 agtttcattt tatcttccta ctttcttttt acatatctat tttcctgtta ccttaagatt
    26941 aaatgagaat ttaaaaaaat gaaactaact tgctttgtca ttatgaattt tttcaaaaaa
    27001 cattcttaag aaaaaggtgg ctatatatag tagtttttgg tcaatacctt tgaataaaaa
    27061 gccatgaaaa acgaatagtt agcgcttacc gatgcgtttt gtgataaaaa aattttaaaa
    27121 tattcaatga aaacaaagca tatattttgc tttaaaacca aatgaggaat attctagatg
    27181 tagaacaggt tagaaaaaga ttagaaaaag gagtgatttt gatggagaag agatacggcg
    27241 tagttcctgt ggtaactgct tcagacgaaa attatgctcc ttacttgagc gtgatgatcg
    27301 caactgcatt agaaaattgc aataaaacaa gacgcataaa attttatgtg attgacgatg
    27361 gtctatcaga atatagtaaa gaaggattgg aagaaacggt aaataaatat tcaagtaatg
    27421 caagtatcca attcctgaca gtggaaaaag atatctacga agacttccta gtcagtgacc
    27481 atatcacaac taccgcttac ttaagaattt ctttaccaaa cttattagct aaagaggatt
    27541 ataagaaagt cctttactta gattcagatg tgttagtact agacgatatc gtaaaactat
    27601 atgatgagcc gttaaatgga aaaacaatcg gcgctattat cgatccaggc caagtgaaag
    27661 ctttggaaag attaggaatc gattccgatg acttgtactt caattcagga gtgatggtga
    27721 ttgacattga tcaatggaat aaaaaagaaa ttacagaaaa aactatccat tacctcagtg
    27781 aaaatggcga tcgaatcatc tatcacgacc aagatgcttt gaatgctgta ctttatgaag
    27841 attgggaaca acttcatcca aaatggaaca tgcagacttc gctgattttt gaacgtcatc
    27901 cagcaccaaa tgaaaaatat gaacgtcagt ataaagaagg aaatgaaaaa ccttcgatcg
    27961 ttcactttac aggacatgat aaaccttgga atacgttgaa agatcatccg tataccaatt
    28021 tatacttaaa aaaattagca cacagtactc taacgaaagt aggcgagatc aatgaataaa
    28081 aaagaaatag cagtagtcgc aagttgtaac acaaaattcg taccacattt agctgcatta
    28141 tttgtctcag tattagataa ttgcaacccg tctaaatttg ttcggttcta tgtgattgac
    28201 gatgatatcg attttgaaag taaacagctt ttacgcttct cagtgaaaaa cgcacgaatg
    28261 aactcagatg tggaattctt gaaaatcaat aaagaattct ttacaaatgt ggtaatcagc
    28321 gatcgtattc cagaaacagc ttattatcgt attgcgattc cggaattgtt ccgcggcaca
    28381 gaagttgaac gtatcttata tatggattgc gacatgatcg ctttacaaga tatctctaaa
    28441 ttatggcgtc ttgacttcgg tgattcaatc gtagcagctg ttgaagacgc tgggttccat
    28501 caacgactgg aaaaaatgaa aatttcagct aaatcaatgc gttatttcaa ctcaggtttg
    28561 atgctgatca atgtgaaaaa atggttagat gaaaatatta cgcaaaaagt attagatttc
    28621 atcgaacata atccagaaaa attacgattc catgaccaag atgcattaaa tgctatctta
    28681 catgaccgtt ggttaccact tcatccgcgt tggaatgcac aaggctatat catggcaaaa
    28741 gcaaaaaaac atccaacagc agcaggtgaa cgtgaatacg aagaaactcg caacaatcct
    28801 tacattatcc atttttcagg acatgtgaaa ccgtggagca aggattttga agggccaacg
    28861 aagaaatatt acgaaaaata tgcagggatg acggctttcc gttgtgtagc taaattccct
    28921 aaatatccaa aatatgcaaa agttcaacca aaaacagatg tagttgttaa ataatagaag
    28981 acaacaactg aagaggggga gtaggaatgg aaattacaat gtctaaaaat gcagtagaga
    29041 cgttaattga aaaggttggg gaaaatacaa aaatcgcact cgcgcttatc aatgattccg
    29101 atccatttct aagggacaaa ggtgcttttg caaaaggcag cttttttcaa atcattcctt
    29161 ttgtttcgga atttggagaa tatgcgacta aaatcgagca tccattattg gacatctata
    29221 catctaaatt agagcaaaat tatttcggta aacgattgaa catggacttc aacaaacagc
    29281 ttgacagctt ttcattagaa aatgaaatcg cagtactcga ttataatatc aaattaaaga
    29341 actgcttctt cagctaatag tttcgagaaa gtccaaaaag gaggtataca ttgagatact
    29401 actttgtgga tgaagaaaag aatgtaatag aaacagatga aaaaaaatat aattggctcg
    29461 tacttgatag tgcaaagccc gaagagattg agcaagcaat cgaagaattc gagcttcctg
    29521 atgatatttt cgtaggaacc gaatatccgg aagaagtttc tcggatggag cgcctttatg
    29581 agacgaaact agagcatcca ttttctttgg tagtgattaa tctagatcag tcagataatc
    29641 gtatcgaaaa aaaattaacg ccaatatctt ttgtactttc cgataatctc ttgattactt
    29701 gtatcgatag aaaaaccgaa tttgtggatc ggctgctgac aaagcatgga aatcatttaa
    29761 actcgtttga aaagatcatc acgtatacat tattagatat ttacacccat tacgtgaaag
    29821 aactaagaga gatgaaaaag cgaattgacg ctttggataa agcagctcgt aaaacaacgg
    29881 aaaatgaaga actgtataaa caggcagatc ttgaaagaga catcgtatat atcgatcata
    29941 ccttaagaga tcaacgggaa acaatggatc aattatggaa ttctgaaact ttcatcaaac
    30001 gtttagacga tgaacagcta ctctatgatg tccatttgag acagcgacaa acagaaaaaa
    30061 tgatcgagat ctatcgagat ttattagaga gtattggtga tctattcaac gggatgatgg
    30121 acaataatct aaatcatatc atgaaatatc tagattcagc tgcattaatc atttccgttc
    30181 cggcgttgtt cgcagggatt tggggaatga atacaggagg attaccgggt aaaagttctg
    30241 ctctaggctt tatactcgta gtctttggct ctttgatagc agcagtagtt ttaggttacc
    30301 atttataccg gaaagattat acaaattaaa gaatctattt aaaacaggta tcaaacaaag
    30361 aaatttgttt tggcaataca atttaaaaac taggaggaat acagcaatga cagaaccaaa
    30421 attaatcgac ccgcgaaact tgtaccatac ggagaaattt cctaaacaag atcaggatac
    30481 accagcttta cagcagaata tgattcctgt tcctgattgt ggagaagaat cctatgaagg
    30541 caataatcaa ctagaaaacc gccgtgtact aatcactggt ggagattcag ggatcggtcg
    30601 tgcagcagcg attgcatttg ctagagaagg ggcagatatc gctcttcaat tcttcccagg
    30661 agaagaaaaa gatgcagagg aagtagcaaa atacgtgaaa gttgcaggac gtaagattgt
    30721 cttactacct tttgatcttc gtgacgaaca agcacctaaa gaaatcatag ataaagccgt
    30781 gagtgaactt ggcgggctag atacgatggt actaaacgca ggacagcaaa tttcttgtca
    30841 gtctattgct gagttaccac ttgagcaagt caaagacaca tttatgataa acatcatcgc
    30901 tatgtttgct ttagtgaaag aagctgttcc gcatatccca gcaggaggag caatcgtaac
    30961 aacaacttct gttcaagcat tcaatccaag cgaacattta ttagattacg cagcaacaaa
    31021 agcttcaatc gctaacttta cgatcggttt agcaaaacag cttgcaccta aaggaatccg
    31081 tgtaaatggt gtagcacctg gtccaatctg gacaccatta caactagacg aaggacaacc
    31141 ttctgattcg atccctgaat ttggtcagca aagtttacta gaacgtgcag gtcagccagg
    31201 agaattagca ccagtttatg ttttcttagc atcgaataaa gcaagttatg ttacagccca
    31261 aatttacgga gtaactggtg gagaagcgat caacttataa gaagttgatc gtttcacccc
    31321 tatgaggagg tgcgaaaatg aataaaggtg taaaaacgat cggagtgatc ggaacattac
    31381 ttttgctatc tgtgcttttg tttgcgatac tattaagtgc aacctatcca ttgccttttc
    31441 agctagagaa gtttcgattc ttttccatca ctaattcata cgtgcaacaa tatgtatttt
    31501 ggcttgccgt tgcatttgct attttgacaa ttatcacgtt acttgttttc ctgttttatc
    31561 caaaaacaat cggaaacttt ctactgaaac atggtgatgg ggagttgacg ttggataaaa
    31621 aagcaattga aggaatggtt cgttctcact tgcatgaaga tgaattcatc cattcaccaa
    31681 aagtaaatgt caaagcaacg aaaaacaaga tcgccgtcaa tgtaaaaggg gagttgaaac
    31741 gaacttcagc actcattggt aaaacaggaa cattgatgaa agaaatcgaa aatgaagtac
    31801 aacacatatt gggaacaaaa gagccagtga aagtatcggt gaaatacaca ggctttgaag
    31861 aaccagctaa acgaagcaat gaccgcaaac gagtagaata gggggggacc aaaaatgaaa
    31921 gaactatttt cgacatacaa gctccctatc atctttgggg tacttggatt gatattagcc
    31981 gttttatgga tcaccgtggg ctttttcaag accttattga tcttgatcat ggcagcgatc
    32041 ggcgtcgcta tcggttttta tctgaaacaa acgaaaatct tagatcacta ttttaaatct
    32101 taggaggaaa catccatgaa taacaataac aaaaataata acaataaaat gaacaaccaa
    32161 aaaccagtgt ctgcacatcc agacccaacg acaccaaacc aatcaaatgg tgtgaacgat
    32221 gaaaagaaca aaacagcacc agtatctgca catccagatc cagtagtccc taacgttcaa
    32281 caaggagtag aagagaagaa acatgatgca gaaaagcatg cacctcatca tccaggaact
    32341 ccagaatcta aagttccaga acaacatgat gcaatcgatc cttctaaacc tgtggaagag
    32401 caaaaacatc cttctgacca tgcgaaagca caagaaaaac ttagttctga ccacacaaac
    32461 ggacaaggaa aacttggttc tgatcataca aataaccaag gaaagcatga ttctgaacag
    32521 gaagattcaa tgcgcggtga gctgaattat gaagataaag ttgtgcaaaa aatcatcgga
    32581 atcgcaattg aacaagtaga cggtttatta tctgcaaacg gtggtttctt ctctaatgtg
    32641 gcaggaaaac ttgtgaatac agataatgta acggctggga tcgaaacaga agtcggtaaa
    32701 aaacaagtag cagttgatat ggatgtcatt gtggaatatg gcaaagatat cgaaaaaatc
    32761 tttgaagaaa tgcaagaagt aatcggcaaa gaagtaaaaa atatgacaca tttagaagtg
    32821 atcgaagtca acgctaacgt agtggacatc aaaacaaaag aagaatttga aaaagatcaa
    32881 gaaacggtgc aagacaaagt aactgacgca gcgaaaaaaa acggtgaatt tgcttcgaat
    32941 caaacagata aagtaaaatc agcagctgga agcggcgcac aaaaagtaaa agaaaataca
    33001 gaaccacggg ttcagtaata aggaagtaaa acattaggag gaacgaaaca tgggatttat
    33061 ttggtcactt atcgtcggag gaattatcgg agcaattgca ggagcaatca cgaataaagg
    33121 ttcttcgatg gggattattg caaatgtcgt agcaggttta gttggatcag cacttggtca
    33181 agcattatta ggttcttggg gaccaagtat tgcaggaatg gcaatcgtgc catcattgat
    33241 tggtgccatc attttagtac taatcgtatc attctttgct cgaaaactgt aaaaagactt
    33301 ttaaaaagag gtcgtgaaaa agctgttctt catcaagtaa taagaacagc caaacaaaaa
    33361 tagcttctca tatttttcat ttggttgggc atattatcga agatgcaagc ttttgaacac
    33421 tgtttatacg gaataagagg ctgtgacaag acttttgtca cagcctctta tttatgcggg
    33481 caaattccca attagatttt taaaagtttt tccttaaatg tgtctttatt gatacagaga
    33541 agcttcttta ataaaacaat gcctccctaa attttcttga gataggaaag gtgaacatac
    33601 aaaataatac gaccactttg aaaacccaga tgatccacaa atggatcgta tgatactgat
    33661 gaagtaaaaa gcaatggatc agaaactcaa agagattgaa aaagacaagg aagaaataat
    33721 atctcttttt tactcttgtc tgttttagcc aataatagct cgtaaagtaa tctagacaaa
    33781 caaatagaag taaacaaagt ttcgtaagag cagcaatcgt atcatgttgc tctgctagac
    33841 gattgacgag caggaaaaat gttggtaatg gaatatagat cacgagtaat gaaagaaaaa
    33901 agtaaacaat aaatggcata ctattcctcc taattgtgtg agttcgccct cattttaaaa
    33961 taacgatatt tgtttgtgaa gctttttcgt gtcgtaacaa atgttatcta atgtattttt
    34021 aaataaataa tcattattat caaaataatg
//
LOCUS       DACDYI010000024        33839 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.024, whole genome shotgun sequence.
ACCESSION   DACDYI010000024 DACDYI010000000
VERSION     DACDYI010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.024"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            322..1983
                     /locus_tag="IWM52_001130"
     CDS             322..1983
                     /locus_tag="IWM52_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9973395.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2053..2400)
                     /locus_tag="IWM52_001131"
     CDS             complement(2053..2400)
                     /locus_tag="IWM52_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAP9973396.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2484..3425
                     /locus_tag="IWM52_001132"
     CDS             2484..3425
                     /locus_tag="IWM52_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9973397.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3426..4469
                     /locus_tag="IWM52_001133"
     CDS             3426..4469
                     /locus_tag="IWM52_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9973398.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4475..5545
                     /locus_tag="IWM52_001134"
     CDS             4475..5545
                     /locus_tag="IWM52_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9973399.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5535..6488
                     /locus_tag="IWM52_001135"
     CDS             5535..6488
                     /locus_tag="IWM52_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9973400.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6500..7510
                     /locus_tag="IWM52_001136"
     CDS             6500..7510
                     /locus_tag="IWM52_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9973401.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7795..8616)
                     /locus_tag="IWM52_001137"
     CDS             complement(7795..8616)
                     /locus_tag="IWM52_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP9973402.1"
                     /translation="MNRFASKEEEKLYYEHEASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8713..8991)
                     /locus_tag="IWM52_001138"
     CDS             complement(8713..8991)
                     /locus_tag="IWM52_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973403.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9259..11268
                     /gene="metG"
                     /locus_tag="IWM52_001139"
     CDS             9259..11268
                     /gene="metG"
                     /locus_tag="IWM52_001139"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAP9973404.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11747..12334)
                     /locus_tag="IWM52_001140"
     CDS             complement(11747..12334)
                     /locus_tag="IWM52_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973405.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12509..13831
                     /locus_tag="IWM52_001141"
     CDS             12509..13831
                     /locus_tag="IWM52_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9973406.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13986..14762
                     /locus_tag="IWM52_001142"
     CDS             13986..14762
                     /locus_tag="IWM52_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP9973407.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14755..15324
                     /gene="rnmV"
                     /locus_tag="IWM52_001143"
     CDS             14755..15324
                     /gene="rnmV"
                     /locus_tag="IWM52_001143"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAP9973408.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15317..16201
                     /gene="rsmA"
                     /locus_tag="IWM52_001144"
     CDS             15317..16201
                     /gene="rsmA"
                     /locus_tag="IWM52_001144"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAP9973409.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16652..17281
                     /locus_tag="IWM52_001145"
     CDS             16652..17281
                     /locus_tag="IWM52_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973410.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17405..18514)
                     /gene="ugpC"
                     /locus_tag="IWM52_001146"
     CDS             complement(17405..18514)
                     /gene="ugpC"
                     /locus_tag="IWM52_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAP9973411.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18723..19145
                     /gene="mgsA"
                     /locus_tag="IWM52_001147"
     CDS             18723..19145
                     /gene="mgsA"
                     /locus_tag="IWM52_001147"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAP9973412.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19468..20004
                     /locus_tag="IWM52_001148"
     CDS             19468..20004
                     /locus_tag="IWM52_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP9973413.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(20044..21807)
                     /locus_tag="IWM52_001149"
     CDS             complement(20044..21807)
                     /locus_tag="IWM52_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9973414.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21810..23525)
                     /locus_tag="IWM52_001150"
     CDS             complement(21810..23525)
                     /locus_tag="IWM52_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9973415.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(24304..24528)
                     /locus_tag="IWM52_001151"
     CDS             complement(24304..24528)
                     /locus_tag="IWM52_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973416.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            24728..27496
                     /locus_tag="IWM52_001152"
     CDS             24728..27496
                     /locus_tag="IWM52_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9973417.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            27578..27997
                     /locus_tag="IWM52_001153"
     CDS             27578..27997
                     /locus_tag="IWM52_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAP9973418.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            28009..28479
                     /locus_tag="IWM52_001154"
     CDS             28009..28479
                     /locus_tag="IWM52_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9973419.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            28496..29275
                     /locus_tag="IWM52_001155"
     CDS             28496..29275
                     /locus_tag="IWM52_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9973420.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            29262..30083
                     /locus_tag="IWM52_001156"
     CDS             29262..30083
                     /locus_tag="IWM52_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9973421.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            30100..31149
                     /locus_tag="IWM52_001157"
     CDS             30100..31149
                     /locus_tag="IWM52_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9973422.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            31162..32166
                     /locus_tag="IWM52_001158"
     CDS             31162..32166
                     /locus_tag="IWM52_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9973423.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            32799..33818
                     /locus_tag="IWM52_001159"
     CDS             32799..33818
                     /locus_tag="IWM52_001159"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP9973424.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT        11046 a         5997 c         6912 g         9884 t
ORIGIN      
        1 actggctttt gaacaccgtt tattcagata agggactgtg acaagacttt tgtcacagtt
       61 tctttttgtt tattcttttt tatctttgta tggcaataat agacaatata acgaaaatta
      121 tttaatggct tttggaagac catggaaaac cttgtacaaa gctatttttt ctgaaaaatt
      181 ccttgaaacc tcacgaaaaa atcattattt tatgaaaaag acaaaaaaaa gtgttgattt
      241 tttaataatt attcattaaa ctcttaaaca acttcagaaa atattaatta ttctgaaaat
      301 tccaataaag gggtacgtaa aatgaaaaag aaatttacat ttggtttggt tgcggtatgc
      361 ggccttgtac ttgctggatg ttatggcggc agttcagcca caagcagtag ttcagcaaaa
      421 agcggttcag cagatggagg aggcgtattt aacttagtcg tgcctcaaga aatgcctaca
      481 gctgacttat cagttgcaac agatacaatc agttttacag cattgaataa cgtttatgaa
      541 ggaatttacc gtcttgatga agacagcaaa ccacagccgg caggagctag cgagttggca
      601 gaagtcagtg aagacggatt aacttacaaa atcaaattac gagaagacgc aaaatggtca
      661 aatggtgatc ctgttacagc agcggattac gtttatggct ggcaacgaac agtagaccca
      721 gctacagctt ctgaatatgc ttacctgtat gcgccagttg aaaatgcaga agacattaca
      781 gctggaaaga aagataaatc agaactaggg atcaaagcag ttggtgacta tgaattagaa
      841 atcaaattga cgaaacaaac accatatttc caatatctgc ttgctttccc atcatttttc
      901 ccacaaaaac aatccgtagt ggaagaaaat ggggatgcct atgcatctgc aagtgataaa
      961 gctgtttata atggaccatt cacattagaa gattttgatg gaccaggaac agatacagaa
     1021 tggacttata agaaaaatga tcattactgg gacaaagata cagtaaaact ttctgaaatc
     1081 aaagtcagtg tagtaaaaga atcttctact gcattgaact tattcaaaga tggccaagca
     1141 gatgacgtga tcttgtctgg tgagttagca cagcaaaacg caaacgatcc agcttatact
     1201 tctgttaaag aagcgcggac aagctatatc gaattcaatc aacgtgaaaa gaattcacca
     1261 ttcaacaatg tgaacctaag aaaagctatt tcttattcta tcgatcgtga agctttagta
     1321 aaacaagtac taggtgacgg atcagttgtc tctacaggat tgatcccatc agatatggta
     1381 aaaagtccaa caacgaacga agactttgca aaagaagctg gaaaattagt ttcttatgac
     1441 aaagataaag caaaagaata ttgggaaaaa gcgaaaaaag aattgggcgt tgattcttta
     1501 gaatttgatt tgatggcttc tgatgatgat tcatcgaaaa aagtaatcga atatgttcaa
     1561 aactcaatcc aagaaaactt ggatggcgta acagtcaaac caacacctgt tccattctct
     1621 gtacgtcttg accgctcaac ttcgggtgat tttgacgcag tactaggcgg atgggctgct
     1681 gactatgcag acccaagcag ctttactgat ttgtttgtca caggtaattc atacaaccgt
     1741 ggtcgctgga gcaatgcaga atatgacgaa gcagttaaag cagcttcatc aacagacgct
     1801 ggcgatgaac aagcccgttg ggatgatttc caaaaagcaa tcaagatcat ctctgatgat
     1861 atgggtgtcg ttccggtgta ccaaaaagca gaaggtcacc tagtgaatga aaaaatcaaa
     1921 ggtatcgttc accatgcggc aggcgcttca tgggattaca aatggacata cgttgaagaa
     1981 taatcgttaa taccgtgagc atgagaaaaa acgaggctgc ggcaaatgtc gcaacctcgt
     2041 tctcatattc acttatccta acaaagaaaa aagcatgaaa atagcagaaa gagcaattag
     2101 tattccggca atgatcagcc aaaagctgta cataccgtag ctggcagacg agagcgagga
     2161 aaattctggt tttttctttc ggttccttgt tcgtgcaaga tggaccgatc gctgaaaatg
     2221 atcgaagaaa cccgaagaaa agacccataa aaaaccgccg ataatcagaa acggcaatga
     2281 ccataagaat agatcattag ataattgaat gagtgtaaag gaatggttga tgaggttttt
     2341 tattaaaata accagaatca gtagaaaagc tactgcgtag gggatagatt tttttttcat
     2401 ttgattgctc ctttattttt ggttcattat ctaataatgt agcgaaacat ctgtcagaag
     2461 tcaaaagcta gggggaaaca ttagtgaata gttatatcaa atatgtatta aaacgtgtct
     2521 ttttcatgat catcacgctt tggttaattg caacaatcac ctttttcttg atgcaattgc
     2581 tgccgggcac gccatatacg aatcaggaac gtttgagtcc tgaaacaatc gagatgctaa
     2641 ataaacaagt cggtttggat aaaccagtta tcgtacaata tgggatttat ctttctaatt
     2701 tgcttcaagg agattttgga atttccttcc aatttaaaaa ccagccagtc gcacatttgt
     2761 tagctggaag aatcggacca tccttgcagt tagggttaca ggcaattatc tttggaacag
     2821 tcctagggac gattttagga acgatctctg ccatgaagca aaatacatgg gcagatacat
     2881 cttctacact cgtagcgata ttaggtcgtt ccattccaaa ctttgtattt gctgttttat
     2941 tacaatatat ctttgcaatc aaattacaag ttttgccaat cgcaaaatgg gatggctttg
     3001 tgtacacaat cttaccaaca attgcgttgg ccatgtcgcc gcttgctgac tctgctcggt
     3061 ttatccgtac ggaaatggtg gaagtactgc atagtgatta tgtggaatta gcaaaagcaa
     3121 aaggattgag ccgttgggaa atcgccttta aacatggtct tcgaaatagt ttgattccat
     3181 tgatgacttt attaggaccg ttagctgtag cattgatgac cggatcactg gtggtagaaa
     3241 atatctttgc tattcctggt atcggggaac aattcgttaa gtcaatcaca acaaatgatt
     3301 atcctacgat catggctgta acgatccttt actcatttat gctgattttt gttattttga
     3361 tcgtggatct attgtacggt ctagttgatc caagaattcg tgtttcagaa gggagccgag
     3421 gataaatgga aatgatacca aagcgttatg aaacagttgc agatatccct gcggatgaat
     3481 ttcttccatt acaaaaaaat accgaagaag aacgtgaaca gattactgca ccttctttga
     3541 attttatcca agattcatgg cgccgtttaa agaaaaacaa agctgccgtt atttcaatgg
     3601 ttttactatt ggtcatcatt ttgatctctg tgatcacaat ctttgtttct cctcatgatc
     3661 caactgccca aaacgtggct tatatcaatt tgcctcctcg tattccggga atcaatatcg
     3721 atggattgaa tggaaaagca atggttggtg gtgaacttgt tgataaatat gctcaagcaa
     3781 acgtcccttc aaacgtaaat ttctttttag gaacagacgg tttgggacgt gatgtattaa
     3841 gccgcttatt tatgggaatg agaatctcgt tgctgatcgc atttattgct gcattactag
     3901 atgtaacgat tggtgtagct tacgggttgg tttctggttt gttaggcgga cgtgtcgata
     3961 acgtgatgca acgttttttg gaaatcttat caggaattcc aaacctcgtt gtcatgattt
     4021 tgatgttggt cgtttttgaa ccaggaattt tttcaatcgt ggctgctatg gccatcacaa
     4081 actggatacc aatggcacgt atcgttcgtg cacaaacaat gaaattaaaa gatcaagaat
     4141 atgttttagc tggattgaca ttaggtgaat caagaatgaa aattgcattc aaacatattt
     4201 tgccgaatat ctctagtgtc attattattc aaatgatgtt tagtattcca tctgctattt
     4261 tcttcgaagc ctttttaagc tttatcggtt taggtcttac gcctccgtca gcttcattag
     4321 gaacgatgtt gagcgaaggg tacaagacat tcctgtatct gccttatcta ttatggattc
     4381 cggcagtaac tttatcggtt atcatgattg gctttaactt acttgctgat ggattgcgtg
     4441 atgcctttga tcctaaaatg aaagagtgaa gacgatgacg aaaattttag aagtgaaaga
     4501 cttagaaatc tcttttgata cgtatgctgg aaaggtacga gcgattcgcg gtgtcaattt
     4561 tcatttgaat aaaggagaaa cattggcgat cgttggggaa tctggaagtg gaaaatcagt
     4621 gacgaccaga agcatcatgc gcttgctgtc aagcaacgcc aatatagatg ctggtcaaat
     4681 cctctttaaa ggtaacgata tcgttggaaa atcagaaaga gagatgcaaa aaatccgtgg
     4741 taaagaaata gcgatgattt tccaagatcc aatgacctct ttagatccga cgatgcccat
     4801 tgggaagcaa gtcgctgagt cccttagaaa gcataataaa gtttcaaaaa aagagggaca
     4861 aaaagctgca ttagaattgc ttaaattagt tggaataccc gaagctgaga aacgtatcaa
     4921 cagttatccg catcagttct caggaggtca gcgtcaacgg atcgtgatcg cgattgcact
     4981 ggtatgttat ccggaaatcc tgattgctga tgaacctaca acagcgcttg atgtgactat
     5041 tcaagcacag attttggagc tgctgaaaga aattcagaca aaaatcgata catcaattat
     5101 ctttatcact catgaccttg gtgtagtagc gaatgtcgct gatcgtgtcg ctgtcatgta
     5161 tggaggaaga atcgttgaag ttggaacatc ggaagaaatt ttctacaacc cgcaacatcc
     5221 ctatacttgg ggactgcttg gttcaatgcc gacattagat agtgcgaacg atcgattgta
     5281 tgctattcct ggttctccac cggatttatt gaacccgcca aaaggagacg cattctatcc
     5341 aagaaatgaa ttcgcgatga aaatcgatgc agaacaagaa ccaccatttt ttgaactatc
     5401 aaaaacacac aaggcagcaa cttggctact tgctccgcag gcacccaaag tgacaccgcc
     5461 agaagaaatc caacgtcgct gggctatctt taaagagaaa caaaaacata cttacggtca
     5521 aggaggaatt gctgatgcca aataaactac ttgaagttaa aggattgaaa cagtatttca
     5581 atgtaggaaa aaagaatgaa gtacatgctg taaatgacat cagctttcac atttatgaag
     5641 gagaaacatt cggtttagtt ggagaatctg gaagcggaaa atccactact ggccgtacga
     5701 tcattcgctt gaatgaacct actgatggtg agatattatt cgacggtcaa gatataacga
     5761 agatcaaaga taaaaaaggt ttgacgaaat tccggcatga tgttcagatg atctttcaag
     5821 atccatacgc ttcgttgaat cctcgtatga aagtcagaga tatcattgcc gaaggaattg
     5881 atgttaatgg actagctaaa tcatctgaag agcgtgcaaa aaaagtcgat gatctattga
     5941 gaactgttgg attgaatcca agccacggta ctcgttatcc tcatgaattt tcaggtgggc
     6001 aacgccagcg tattggtatt gcacgagcat tggcagtaaa accgcgtttt atcatttgtg
     6061 atgaaccaat ttctgcacta gatgtgtcga tccaagccca agtggtcaat ttgttgcaag
     6121 atctccaaaa agaacatcaa ttgacctatc tatttattgc acatgatttg tcgatggtta
     6181 aacatattag tgaccgaatc ggtgtaatgc ataacggctt gttattagag atgggaacaa
     6241 gtgatgaaat ctataatcac ggtgttcatc cttatacaga aagtttgtta tctgctattc
     6301 ctctaccaga tccagaccat gagagaaaac gcagaagaat caaatatcaa cctgaaccag
     6361 atgacggaca agtacgtcaa cttcgtgaaa tcgcaccaga acattttgtc tatgcaactg
     6421 agcaggaagt cccatattac gcaaaaaaac taaaacgaca aaaggaatct cttctggttg
     6481 ctaactagga gggacataga tgctaaaagc ttttaaattc cgtatgtatc cgacagaaga
     6541 acaaaaacag caattgattc gtacttttgg atgtgctcga tttacctata accatttgct
     6601 gaaaaagcgg cagaaaagtt ggcaacaaac aggagtagcc aatttttcgc tcacacccgc
     6661 tacattaaaa aaagaatacc cattcttaaa agaagtagac agtcttgctt tagcaaatgc
     6721 acagcttaat ttggatcggg cttttcgcaa ttatttcaag ggacgtgcca gcttcccaaa
     6781 gcttaaaacg aaaaaaagta tgtggcaatc ttatacgacc aacaaccaaa cccgtacgat
     6841 ctatctggaa aatggctact taaagttacc aaaacaaaaa gaactaatca agataaatca
     6901 gcatcggcca gttgaaggat cgattcgctc cgctacgatt tctgctcgat ataatgaaga
     6961 attttatgtc gcactacttt gtgatgtttc gccagtaaaa aaagaatctt tagctaaatg
     7021 gatcgggatc gcctatcatc cgaagacatt gatccaaaca tctcgacctc tagaagtaac
     7081 tttaccaaaa tttcaccaga cagaagaaaa gctgcaacat gcacagcgga aattgaatgt
     7141 gaaagtccgc tccgctcacc atagaaagat caggttagat caggcaagca attatcagaa
     7201 acaaaagaga aaagtaatgg atctttatct gaaacaaaag aaccagcgag aagattatct
     7261 tgaacaatta tctgggaaat tggtcaaaca atatgactac ttatttgtag aatcttttcc
     7321 aaaagaagag gcacatgctg atttttctat tcacgactgg cataaattaa taacaaaatt
     7381 gcgttataag tcccaatggt ataataaaaa atttcttttg atcaataccg acggagcaga
     7441 agaaagcaat tccgtaagaa agagccaagt agtagaaaaa atgggcagac attcggttat
     7501 caagggataa aaaatgaatg aaactgatag gagcgttagg gttagccttg ttaataaaaa
     7561 gtcttaatga cttttgtttc taggaatttt tcttttgaag aaaagctcac taattatgac
     7621 aaaccaataa aacaatagca gaaaaagttc gtacgagctt tttctgctat tgttaatttt
     7681 ttattcagaa agtttacgaa tacatcaaaa ttctgaaaca aaaaacgtaa ctcacaaaga
     7741 gttgtttttg cgttttttct tgaatgatta ggtctgaatc ttttgctcac aaccttattc
     7801 atggtgcccc ggcttgagct gatagatttt tgaaggacgg ccgattccta caggacgttc
     7861 accgatctca tcgaaatatt ggagcatagc tttcttaaaa ttggaatgat cgatcgtttt
     7921 ataatcgatc cctaagaatt tagcaaatac ttttcgagct tccgtaatcg tgaaatcttt
     7981 acctaaaact tgtagaactt gcggctcgtg ttccatttta ttgactacac ggttgaatgc
     8041 tttcaagatg atttgactat ggtcaaaagc caaagtatct ttgccagaag actctccagt
     8101 gactaaatca agcgagatag ccacttctcc gctagataaa tggatcttgt tactatgacg
     8161 ttctagtgtg aaccagcgta cttccttggc atcatctccg gcgattagcg gttcttctcc
     8221 aataaaggct aagtagctga ctgtgacgac ccagcctcgc ggatctcgat taggcgtact
     8281 gaatgtatgc agttgctcaa tattttcttt tgaaataacc acaccagttt cttcctttgt
     8341 ttctcttaag acgctttctc cagttgactc atccttttgg acaaagccac caggcaatgc
     8401 ccatgaattt cgataaggat gcccttttct ttggatcaac aaaactttca gctgatcttc
     8461 ttctttgtta tagcacatca gtacgatatc gacagtcaaa gagggcttgt catattctgg
     8521 acgttcctgt ttttgatacc agttaagaaa ttcttcttca cttgcttcat gttcataata
     8581 taatttttct tcttctttag aagcaaaacg gttcatctga ccacctccat tgacagtaaa
     8641 agtacttttt accttattgt agcagactct gaaaaaaaac caaggattta cttgaaaaat
     8701 ctaggttatc atttaaatat gaaaaataaa tggaagtatg agattgaata aaaagaaaag
     8761 cagataaaac ggcaatactt tataccgctc ttcacgaatg gcaaagaagc cacaaataac
     8821 gatatatacg tcaatgataa aacgcaaaat ccaagctgct gtcataaaac tgttcaaatg
     8881 cccatggagg ttaaaataac catacatacg aatataaaaa tagctgaata ttaaatgtgc
     8941 taatgaaaga aaagcaatac taagtgtaaa aaaaagatgt ttcttctgca tgattgttcc
     9001 agtcccttac taagatagat agtctttatt ataccacgtg tgaggggaga aataaaaagc
     9061 cgttttttag tagatatgaa acaaaacaaa aacccgcatt tattcaacct attcatcctt
     9121 ttaaaggata gattgaacgt agatagaagt cgtttatcca gtctttttca ttttgaaaaa
     9181 aagctaacag catgcttttt ttatggtata ctagcaagga aacgaattcg catcatgcga
     9241 taaaggagga agagccccgt ggcagaaaag gaaacttttt atatcactac gccaatctat
     9301 tatccaagcg gcaaacttca tatcggcaac tcgtatacta cgattgcatg tgacgcaatt
     9361 gctcgttata aacggttgat gggctttgat gtgttttatc tcactggagt ggatgaacat
     9421 ggtcaaaaaa tcgaaacaaa agcagaagaa ctaggtgtaa aaccgcaaga atatgtagat
     9481 aaaatggcgg ctgatgtcaa gaaattatgg aaaacgcttg atatcagtta tgataaattc
     9541 attcgcacga cagatgatta ccataaagca gctgttcaaa aaatatttga tcgtttatta
     9601 gaacagggag atatttactt aggtgaatat gaaggctggt actctgtttc tgacgaagaa
     9661 ttctttacag aaacacaact tgcagaagtg tacaaagatg acgaaggtaa cgtgatcggt
     9721 ggtaaagcgc caagtggaca cgaagtagaa ttggtaaaag aagaatcata cttcttccgc
     9781 atgagcaaat acgcagaccg tctattggaa tactatgaag aacatcccga attcatccag
     9841 ccagaatctc gtaaaaacga aatgatcaac aactttatca aaccaggatt agaggatctt
     9901 gcagtttcac gaacaacttt tacttgggga gtaccagtca agagtgatcc gaaacatgtg
     9961 gtgtatgtat ggatcgatgc attatccaat tatattactg cactaggata tggctccgaa
    10021 gatgaaagtc tattcgaaaa atactggcct gcagatgttc agatggtcgg taaagaaatc
    10081 gttcgctttc atacgatcta ttggccaatc atgctgatgg cccttgattt gcctctacct
    10141 aaaaaagtat ttgggcatgg ctggctattg atgaaagacg gaaaaatgtc taaatcaaaa
    10201 ggaaatgtcg tttatcctga aatgcttgtg gaacgttatg gtttagatgc actccgttat
    10261 tatttgcttc gtgctgttcc gtttgggtca gatggtgtct ttacgccaga agattttgtt
    10321 tcccgtgtca attatgatct ggcaaatgat ctagggaact tattgaatcg aacgattgct
    10381 atgatcaaca aatattgtga tggactcgtt cctgactatg cctcacttgt cactccattt
    10441 gatagtgaac tgtctacgac ggctgctaac gttgtcggac gttaccatga tgcaatggaa
    10501 aaaatggaat tcaatacagc attggctgaa atctgggtgc tgatttctcg tgcaaacaaa
    10561 tacattgatg aaacagaacc atgggtatta gcaaaagacg aagaaaagaa agctgaattg
    10621 aatagtgtca tgatccattt agctgaatct ttgcgtatcg cagcgatatt acttcagcca
    10681 atcatgacag aaacgccaga gaagatcttc aatcaactag gattggactc tgaaacaatg
    10741 aatcttgaag gattacactt tggcgaattt ccttcaggaa caaaagtagt tgcaaaaggt
    10801 acaccaatct tcccacgttt agatatggaa gaagaagttg cttttattca agaaaaaatg
    10861 tctgaaggta ctcaaacaaa cgaagataca gtcaaatggg atccagaaga aacagaactt
    10921 gtttcaacga aagaaaaaca aatcaaattt gatgtatttg aaaaagtaga actaaaagta
    10981 gcagaagtca tcaactgtca aaaagtagaa ggggcagata agttgctgca attccgacta
    11041 gatgctggag acagccaaga ccgtcaaatc ttgtcaggaa tcgctgaatt ttatcctgat
    11101 ccaagcgaat tgatcgggaa aaaagtcgtc attgtagcaa atctgaaacc acgaaagatg
    11161 cgtggacaaa tcagtcaagg aatgatcctt tctgcagaag caccagatgg ctctttacaa
    11221 gtaatcgaag caccaaaatc catgccaaat ggctcagaaa tcgcatagaa attcaaaggg
    11281 ggtatgacac tagttgtgtc ataccccctt tttccgaaat ttgagaagca attttcgtaa
    11341 attccttctt attacttgaa gccaatcata taatacagaa acagagaact atccaaaata
    11401 aatcctagaa aaagaagtac gagcttcgct gatttttgtt aatttagaat aatactaata
    11461 ggaatcgtaa aaacagaaaa aaagcataat gataaagggt cgggcagatt ttaattgatt
    11521 catctggtac tcttttttat tgtccttaca ttcataaagt ttgaatgcaa tgactttatt
    11581 acatctatat ttatatctaa cagtagcgtt gatgtgttaa ttataagttc tatcacttta
    11641 tgttcaatta gtttgtgctc ttatatgtgt gattgaaaat atagataaaa aaacaatcat
    11701 cggctttgac ttcgatgatt gttttcttta ttttgtaaaa atcctatcac ttgattttat
    11761 ggtaaccttt tatttttgtc ttcaaacggc ttggtaagag agcagtaagc agtgcaaatg
    11821 ggatcgaaaa gacataccag aaaatcgtac gtccgatacc ataatgttga tagagctttg
    11881 tcaacatcac gtgtaacaag atcaacccta aaagcaagga gccagctata aaccaatgat
    11941 tatcccaatg catgacttca ctaagtgcca tcactccaaa aggatagaaa taaaaataga
    12001 tcccgactaa ggtcatccgc cagttctttg taaatgttcc gatattcaat aaaaagaaaa
    12061 tcgctgaaag caaaataccg ataggcggaa aaagcccaac caaaaaggaa tacaacatcc
    12121 cccacaagat catcacagga cctttgatca atgcaacgat tgcgatgaac aaagtcagca
    12181 atattagttt ttgtgataac gttgcaacag tcattaatcc aaataaaatt aatgtgaacc
    12241 aatgccaatg ggactgctgt tgttctttac gggaaaaatc gacccggttg gataaataat
    12301 ctgctaattt cggataagaa cgcttaaaat ccataaactg ttcctccttc gtggaaaagg
    12361 taggtttagt atacgaaaaa taaaaaagaa atacatccgt cttaagaggg agtttttcac
    12421 tgaaagtgga gaaaaaagaa tggtaaatta aaatattctc agttatactg aaaattatac
    12481 caaatttcaa agaagaaaga ggatgggaat gaaaggcaaa ctaaaacgag aaatcaatct
    12541 gtttggcgca ttagctaccg ttatgggaac cgtgatcggt gcaggcgtct tcttcaaaac
    12601 tgctgctgtt actgctagta ctcagtctac aagtttgaca ttgctagctt ggcttttggg
    12661 tggtttctta acgatctgtg ctggattgac agtggctgaa ttagctacag caatcccgga
    12721 aactggtgga gctgtaaaat atattgaagc tgcgtatgga aaattgccca gctttttatt
    12781 aggatgggcg caaagcttga tttactttcc tgctaatatt gctgcattat cgattatctt
    12841 tgcgacacaa ttgacgaact tattacaatt atctaccgat tatcttttgt tgattgcagt
    12901 gatcaccgct gtttctgtga cagggttgaa tttgttaggg acaaaagtcg gtgcttctgt
    12961 acaatcagtg acgttgattg ttaaattaat accaattgct gtgattgtca tatggggatt
    13021 attgacccca ggacaaggaa ctgtccagtt atttccaatc gaagctggaa aagatgttac
    13081 ttttgtcgaa ggattgagca gtgcgttgtt agcaacgttg tttgcttatg acggctggtt
    13141 aggtgtcggt gcaatggcag gggaaatgaa acggccggaa aaagatttgc ctaaagcaat
    13201 tattcttggt ttgagttttg taacagtcgt ttatctattg atcaattttg tttttttgaa
    13261 aactttgccg attgatcatc ttgccggtaa tttgaatgca gcttctgaag catcagacgt
    13321 catttttgga ggaatcggtg gaaagctggt cactattggg atcttgatct cggtatacgg
    13381 tgcattgaac ggttatacac taactgggat ccgcgtgcct tatgcgatgg ctttagaaga
    13441 cgagctacct ttcagtaaac agctgacaaa gctttcaaag aagtttacgg ttccttatgt
    13501 tcctgcggtt ttccagttag cagtagcttg tattatgatg agccttggtt ctttcgactt
    13561 tttaacagac atgttgattt ttgtgatgtg gctattcagt ttactgattt ttatcggtgt
    13621 ctttatttta agaaaaaaag cgccggaatt gccacgtcca tataaagtac cactttatcc
    13681 aatcgttccg attattgcga ttctcggtgc tattttcatt ttaggtatga cgatgatgac
    13741 gcaaacgaaa ttagcattga tcggtatagg tgttaccttg ataggtatcc cagtctatta
    13801 tcaaaaaaag aaaaaacgct cagaagagta aaatatttca acaacagaca gaaaaagtac
    13861 gaactaggtc ctctagatct tcgtgctttt tctatttcaa taaaacaagt tcttctattt
    13921 tgatttttgc tacttctgca agtagtgctt atgtgctaaa gtaagaatga aaaggagaga
    13981 taaacatgat atttgattct catacccatt taaatgctga acagttcaat gaagatattc
    14041 cagaaacgat tcagcgagca caagaaatgg gggtaacgaa aatggcggtc gtgggcttcg
    14101 atacgccaac gattgaaaag tctttgcaat taagccatga ctatccaaat atatacagta
    14161 ttatcggctg gcatccgaca gaggctggca gttatacgaa agaaatcgaa aataaattac
    14221 aagaacaatt gactttacca aaggttgtag cattagggga aattggcttg gattatcatt
    14281 ggatggaaga cccgaaagaa gtccaagata aagtatttcg cagacagatt gctatcgcaa
    14341 aagaaatgaa cctaccaatc agtattcata ctagagaagc acttgaagac acgtatcaaa
    14401 tcctaaaaga agaagatgtt cgagatatcg gtggaatcat gcatagtttc agtggtgatt
    14461 atgaatgggc aaaacgattt cttgacttag ggatgcatat ttcatttagc ggtgtcgtca
    14521 ctttcaaaaa agcattagac gtccaagagg ctgcagcaaa tgtaccaatc gaacgattac
    14581 ttgtggaaac agatgcccct tatctggcac cagttccttt tagaggaaag cgaaatgaac
    14641 caggctatac tcgttataca gtagaaaaga ttgctgaact tcggaatctt ccgacagaag
    14701 aagtggctgc ccaaacatgg acaaatgcgc accgattatt tggtctaagc gaacatggat
    14761 aagatgaaga tccaagagat tatagtcgtt gaaggaaaag acgatacaag aaggctgcgg
    14821 gaagtagttg atgcagatac gatagaaaca atagggtctg caatcaatga agagatcctg
    14881 atgcaaattg aacatgccca agagactcgt ggagtgatcg tttttaccga tcctgatttt
    14941 tcaggagaaa aaataagaaa aatcattatg gaagctgttc ctcaggcaaa acatgctttt
    15001 ttgccgcgca cacaagctaa accaaagaaa aagggaagcc ttggtgtcga gcatgcaagt
    15061 gatgctgcta ttttagaagc attgaaaaag gtcgtgacgc cagacatttc ccaaacggct
    15121 gtttctgaaa tcactagaca agacctttta gactatggtt taatagctgg ggcacaagca
    15181 aaagaaaaac gagaaaaact aggtgacgag ttgcgcatcg gttacacgaa cggcaaacaa
    15241 ttggaaaaac gtttgaagat gtttcgtatt acgccgaaag aactcgccca agcaatgaaa
    15301 atagtggagg aacaacatga gtgaatatcg tgaaattgcg acaccctcta gaacaaaaga
    15361 gattttattg aaacatggtt tttcatttaa aaaaagttta ggacaaaatt ttctgactga
    15421 accaaatatt ttaaggaaaa tcgtagaaac tgcgcaaata gatgaacata cgaacgtgat
    15481 cgaagttgga ccaggaatcg gtgcattgac ggaacaatta gccaagcatg caaaacaagt
    15541 cgtagcattt gagatcgatg atcgtttgat ccctgtattg gctgatacga tgcaacccta
    15601 tgacaatgtg actattgtac atcaagatat tttaaagacc gatttgtcaa cagctgttag
    15661 agaaacattt catgaggagc ttccgttaaa agtcgtagcg aatttacctt actatattac
    15721 gacaccgatc atgatgcatt ttcttgaatc agatttggtt gtggatgaac tagtcgtcat
    15781 gatgcaaaaa gaagtagctg atcgtatctc tgctgaacca ggaacgaaag cttatggttc
    15841 attatcgatt gctgttcaat attatatgga agccagttta gcattcatcg taccaaaaac
    15901 agtatttgtc cctcagccaa atgtagattc agcgattttg aaactgacaa gaagagatat
    15961 accggcagtc gaagtgacag acgaaaaagc atttttccga ttgacaaaag cggcatttca
    16021 gcaaagaaga aaaacgcttt ggaataactt gcagcattcg tatggcaaag atgaccagac
    16081 aaaagcatgg ttagcaaaaa gcttggaaac agctggaatc gatccaaaac gtcgtgggga
    16141 gactttgtca ttacaagaat tcgccgcatt aagcaatgcg atgtcagaaa atatgcagta
    16201 aaaaaagagg ttgtgacaga agttgtgaaa aagctgtcct gcatcaagta ataagaacag
    16261 ccaaacaaaa atagttcctc atgtttttcg tttgtttggg cttattaccg cagatgcaag
    16321 cttttgaaca ccgtttaaga aagaggttgt gggcctgccg tttaactcta agtcacaagg
    16381 aacaatttca aaaaacagct tctcatgttt ttgagttagt tggtcttatt acttgatgca
    16441 agcttttgac cactattatg ccgaacaagg agatgtgaca caaattttgt cacatctcct
    16501 atttttgttt cttgatcaaa aaattcttat acttgatact cacactcatt ttgtatggat
    16561 ttagaaaaga atatgtgtct tgacagttat tagtaagtga gttatactaa gtcgacattt
    16621 atgtgtcaag acatattagg agagagaaaa aatgagaaaa aattcagttc gaaaactaac
    16681 gatttctgct ttattgattg ggatgggggt cattatccca atggtcatgc ctaaaatcat
    16741 gattccacca gcttccttta ctttggcgtc tcatgtgcca ttatttattg cgatgttttt
    16801 tacaccagga gttgcagtgg ctgttgcgct aggtacgaca tttggtttct ttttaacaac
    16861 gccggttatc atcgcattgc gagcattgtc acatcttgtg tttgcgttga ttggggcttg
    16921 gtacttgcaa aagcatccga gtatcgtttt aaaaaatgga cagtttactt ttgctaattg
    16981 gcgtttccaa gggttcaatt ttgtgatcgg tgtgattcat tctttagcag aaatgctagt
    17041 agtcagcatc ttttacttta ttggtaatat gcctgaaaca tattattctc aaggatattt
    17101 ttatacagta ttcatcttga tggggattgg tgggcttatc cacagtttag ttgattataa
    17161 tattgcttat tttatagcag gaacactcag caaatatttt gatatccccg tatttacggc
    17221 agcaaaaaaa gaaaatcgga aagagtcgat tcaagggtac gcgaagaaaa cgacatatta
    17281 gacatgatct ctcaaaaaaa aaactctcat ctagtaatgg ataataaatc ctctaagatg
    17341 agagtttttt gtcctaaaaa gagctgcacc aacatcttct ggcacagctc taacaggggg
    17401 caatttatgg aagtttgatg acttgttctg tttctttatc aaagaagtgg cctttgctga
    17461 tgttgaaggc aaggtcgact atttcgcctg gtttatggaa atctcttgca tctacttttg
    17521 agatgaactc agtagaacca gtttttgtgt aaagcatcgt ttcagctcca agtaattcgg
    17581 aaacgacaac ttctgatttc actacggctt caggtgaagc ttcaagggct acttgttcac
    17641 tgtggatgtc ttctggacga ataccgaaga taacttcttt tccttcataa cctttttcaa
    17701 ctaatacttt gttttttcct tcaggaatac gaagcttaag cccatgtcca tctgtgataa
    17761 caccattttt caatgtcaca ttaaagaagt tcatggcagg agatccaata aagccggcaa
    17821 cgaacatatt gacgggtgta tcataaactt ctttcggtgt accgatttgt tggataaatc
    17881 cgtctttcat gataacgatt cggtcagcca tagtcattgc ttctgtctga tcgtgagtaa
    17941 catagatggt cgttgtttca agacgctggt gaagtttggc aatctcagca cgcatggcta
    18001 cacgtaattt agcatctaaa tttgataatg gctcatccat taagaagact tttgcatccc
    18061 gaacgattgc ccgtcccaat gccacacgtt ggcgttgtcc accagataaa gcagctggtt
    18121 ttcgtttcaa gtattctgtc agacctaaga tctctgctgc attgtccaca cgttttttga
    18181 tgtctgcttt atcatattta cgtaatttca agccaaaagc catattatca aaaacagtca
    18241 tatgaggata caatgcgtag ttttggaaaa ccatggcaat atcacggtct ttaggtgcca
    18301 cgtcattcat gacttttccg ccgatattta gttctccttc agagatatct tccagtcccg
    18361 cgatcattcg caaagtagtt gactttccac aaccggaagg accaacaaaa acgataaatt
    18421 cacggtcttt gattgataga ttaaaatcag ttacagaata attttctgca ttgtcatatt
    18481 ttttatatac gtttttcaga gccatttcta ccataatacg ttccacactt tcttttttag
    18541 ttttattaac tatttaaagt ataaatgaaa gcgttaacta acttcaatgt catgttgaac
    18601 aagaattttt tctttttttt gtcaatgtgc tagttttaag ctttaataaa tatccataag
    18661 aaataaaaac tgatacactg gagtaaataa gtatgttaac aatatcaaaa ggagtgtctc
    18721 ttatgaaaat cgctttgatc gctcatgacc gaaaaaaacc tatgatggtg aaattagcaa
    18781 cagcgtataa aacaatattg gaaaaacatg aactttttgc taccgggacg actgggcaga
    18841 ggatcacgga agcaaccggc ttgccgatcc atcggtttaa atccggaccg ttaggcgggg
    18901 atcagcaaat tggagccatg atttcggagg atcagttaga tttggtgatt tttcttcgag
    18961 acccgctggc tgcacagccc catgaaccag atgtaacagc acttatccgt ttatctgatg
    19021 tctatgagat tccgttagct acgaatatcg gaacagctga ggtgctacta agaggactgg
    19081 aagccggctt cctagatttt cgaagaatcg tccatgaaat agacaagcat ccactttcat
    19141 tttgatggat aagcgtatgg aaaaataacg tttaatgttc gcctatatgt agctataaaa
    19201 gctaaacaaa ctgataatat gcgatataat acgaacgtat tggataatat aaacaataat
    19261 attagttttt cgtatttgca gtaaatgtga aaatatgcta tagtttctgt gggaaaagat
    19321 cccaaaaata atacagagta gaaaataaag cgttaagtgc taagaggatg ggatgttgcc
    19381 tcttggacga aggacgtaat gttcgcggtt ttattttcgc attcgctgca tgatacgatg
    19441 tagcagctct taaaggagat gctaaaaatg aagaaacacc ggttagacgc acgtatgatt
    19501 gctattatgg gcttattgat tgcattgatg gtcactttgt cccgactggt agcaatcgag
    19561 accccattta ttaaaatcag tgtcacattt attccacaag tgatcatggg cattcttttt
    19621 ggtccttttt ggtcagggat aggcgcagtt ttagctgatt tagtaggaat ggccctcttc
    19681 tcgaaatctg cattctttat tggatttaca ttgaatgcat ttattgaagg agcaatctac
    19741 ggattctttt tctatcgaaa agagatcact tggaaaaatg ccattctggc tacgcttagt
    19801 gtcacgctga tcatcaactt atttttgaca ccattatggc ttgcgttgat gtaccatgtg
    19861 ccattgttca gctgggtcgt ttgggcgccc cgcctgttaa aaacagtaat atggttaccg
    19921 attcaatcaa ttgccattta ttacgtagga cgtagtattc cttacaaaaa aattttaaga
    19981 agtttagcga tccacgctaa ataaaaaaca agcagtcttc ccatcccaga agactgcttg
    20041 tttttattgt gctgtgaatt gtgcttgata gagccgtcgg taataaccat ctttttgaag
    20101 cagctcatca tgggtgccca tttcgacaat agctccctga tccatcacta atatctggtc
    20161 cgcatttttg attgtggaca ggcgatgggc aatcacaaaa ctggtttttc cttccatcat
    20221 tgctaagaag gcttgttgga tttttttctc tgtcaatgta tcgactgagc tggtagcttc
    20281 atctaagatc aacatagggg gattactgat catcgttcga gcaatcgtca acagttggcg
    20341 ttgtccatct gatatcttta acccttgacc gccaatctta gtatctaatt tttccggcaa
    20401 acgttctaca aattcataca tataagaggc ttttagggca tcattgattt ctgcgtcact
    20461 tgcttccgga ttcccaaaac gaagattttc tcgcaatgta ctgtcaaata gccaagtatc
    20521 ttgtaacacc attccaaaac ttttacgtaa tgtatcacgt tggatagagc ggatgtcatg
    20581 gtcatcgact gtaattgttc cttttgttgt gtcataaaaa cgcatcagca aattaacaag
    20641 tgtggatttt ccggcccctg tttttcctac tatagcaatg gtttgacctg gataagcagt
    20701 aaaattgaaa tcttcgatca aacgctgctt ggggtcataa gaaaaagaaa catgatcaaa
    20761 agagactttt cctttgatat ttttgggttc caaggcatag gcatctggca aatcaggctt
    20821 ttcttctggc tgatcgatca aatcaaatgc tcgttctaga ccagaaaaag cggtttgcag
    20881 ttgtgtcgtg attccagaaa gttcgataaa tggtttagaa aactgacttg agtagatcgt
    20941 aaagctggaa atcacaccaa cagtgattga cgaaccactc ttcaatgcca ataggccgcc
    21001 aactaaacca atcgataaat aagcaaggtg atctacaaaa cgagaagacg ggtttgtcaa
    21061 ggaagaagaa aattgtgccc gttgtccttt ttcatataga tcagcattta gcagttcaaa
    21121 ttgctgttga ctagcttctt cttgctgaaa agctttcacg attttctggt taccgattcg
    21181 ttcggtcaca aagcccgaga tatccccaat aatcctttgc tgtctatcga aatttgtttg
    21241 tgaagcacga gcaacgatcc aactcacaag gaaaataatt ggtgtagaac aaagaacgac
    21301 caatgtcaga agtggactta gacgcatcat aaaaataaac gaaagaatga tgattgccac
    21361 tccagaaaat aattgattga aggccgcaga aattgcaatg gaaatattgt ccatatcatt
    21421 tgtaaatcga ctgacgatat ttccgtggga gttttgatca taataacgaa gtggcaatcg
    21481 attcagatga gcaaatgcat cttttcttaa ttttgcaacg gataagtaag ctaaccgatt
    21541 gcttaacact tggatgagcc attgactgac tacgttgatt aaaacgatac caccgaatag
    21601 aataagtaaa tggaacagtc gattgaagcg gacctgtcct tctccaatca tttgatcgat
    21661 actttgacca atttgtaacg tcatcacaac tgttgcaatc ccattgatga ttcctagtat
    21721 cacagctagc caaatgtctt ttgcataggc agtaaggtaa ggaatgaatc ggcggagcgt
    21781 ctgcaaggat aattttttta ctttcataac tattcttcct cctgtgatgc gacgatttcc
    21841 tgatattctg ctgattgagc aagtaattct tcatgggtac cctgagcagc aatcttacct
    21901 tgatccatca ctaagatatg atgtgcttct tgaatagagc ggacacgttg ggagataata
    21961 atcactgtac tgccaagata agtctgtagc gccttacgca aatccaagtc tgtttgataa
    22021 tccaacgcac ttaaagagtc atctaaaatc agtaatgctg gcttggcaat caaagctctt
    22081 gcaatcgtca gtcgctgttt ttgtccgcca gagaaatttt tcccattttc catgactttt
    22141 gtatctaaac catttggaag ctgacggaca aattcttctg cctgagcgat tttcagagct
    22201 tcccaacatt ctttttctgt ggcgtctttc ttaccccatt gaaggttttc acgaatagtc
    22261 ccggtaaata gtacagaagt ttggggaaca agagcaattt gctgacggag atgatccagc
    22321 tgccaagaac gaacatccat tccgtccatt ataactgtac cggaactgac atcatagaaa
    22381 cgcggaatca attgtgtcaa agtcgttttc ccgcttccgg ttggaccaat gattccaagg
    22441 actgtatttc gcttcagctg aaatgtaata ttttggagag aaagaccaga atcaggagta
    22501 taacggaagt caacatggtc aaaagcgata attgcttcag aagaaaaatc gggagtagta
    22561 ctgacctcag cttcaagaat cgaattttct gtgtctagca cctctttcac ccgatttcct
    22621 gaagcttcgg cacgtgtgaa gatcacgacg aggtttgaaa caacgatcag tgccaaaagc
    22681 atttgattca tataattgat caaagcaaga acttgtcctt gttgcaagct accaatattt
    22741 actttgatcc cgccaactgt cagcaaacag atgatcccga cattcatgat caaggttgta
    22801 gcaggtgata atagggcaga gatattggaa acacgtaaat aattgttcgc tagatcattt
    22861 gtgcttttat cgaacttctc tgtttctttt tttgtctgcg caaatgcgcg gatgacacga
    22921 acaccgctaa gattctgact aacgtgttca ttgagctgat ctagtttttc ttgcactttt
    22981 tggtagagag gaacggtttt tttgatgata aaaaataaaa tgattgagaa aataggcaat
    23041 aatgctaaga aaaacagacc tacttcccag tcgatcacaa aagccatgac cactgacccg
    23101 atacttaaaa acggtgctct gatcaataaa cgaatgacca ttgctaaagc tagctgaagc
    23161 tgattgatgt cgtttgtcat tcgggtgatc aaggtatctg ttccaaaatg attcaactcg
    23221 ttgtgagaga aagtattgat ttttttgatt agctgattac gtaactctgt accaaatcct
    23281 tgtgaagcta ctgaagcata gtattggcaa atgatagcac aaatcagacc aatcacagac
    23341 atagcgacca tccataagcc catttcaatg atttttcctc ggtcattcat ttttagtcca
    23401 ttatcgatca aggaagccat cattaaaggc aggacaagtt cgaagacagc ttcaagaaat
    23461 ttgaagaacg ggcccaaaat gatttgctta cgatagtctt ttgcataacg taataactga
    23521 atcatatgat tcctcctatt atttatttgt ccacaattga aaaaacaagt actaccacat
    23581 tgtagcttag gaaatttaga atgaaaagag aacaagcctt ttcataaatg aaagttttat
    23641 tatgaagaag ctttagaaat tgaatagact atttttttta aaaaaaagaa aatgagaaaa
    23701 catgccagtc tcttcattca taggaacgtc tattttactt aatttttcta taaatgaatt
    23761 ctgcataaaa aatatatcaa gtaaatgttt atagatgaaa ctctatttac ttctttttta
    23821 atttgttctt gcaatctttt ttgctacgta atttttgtaa aaaagtgggt tgagagaaat
    23881 ctaattgatt ttcaaagaag gagtatgaaa atgagaaaaa tatttattcg tttattgtgg
    23941 gtactatctt agaggaataa ataatagtgg agaaaactat ggtttggcgc agattctatc
    24001 aggtttgatg gaacgaagac aacctcccga catcaagtca aaagctactt gagattttga
    24061 tctgaaaata aaaaaagaaa acaaggtagg aacatcaatg tttctacctt gttttcgtaa
    24121 tcaatgggaa aaaactaaga gtggtgtacg taatcaataa aaaggatggt gacaacagta
    24181 cctctttact ctctgaaagc atcataactc gtttttcctc attatgccaa taaattgttg
    24241 ttataccaca tataaggatt tgtaatagct aaaaacagac aggaaagttg aaatctgtga
    24301 atcttatttt gtatggaagg atttatagaa cgcaaaaatc aagataaaga tgaataaaaa
    24361 tcctgtttcg gctatatatt gcgtatctac gccaataaaa cgagtttgtt ctgtcatagc
    24421 catataaagc aaaggcaaaa atacaactac agcgagataa gatccgatga tttctcgaaa
    24481 agttttccag gaagtatgtt tcctgttttt atatttatcc gatttcattg aatagtcata
    24541 gcctccttat tctttcatat gttgaaattc cagtataaaa actggaagga actgtacgtt
    24601 ttttagaata aaaattataa gggataagta ggctgctgtc tataataaga tatatttttc
    24661 aattctttat taaagatttt attgacaaaa tgaatccgtt ttctatgatg gaggaggggt
    24721 gaataagttg ttgaagaagg aaattttatg ttacttagag aaccagacag cctttttcga
    24781 tttagaacat atgaacgagg tctttacagc acaaaggtta gctgatcatt ttggggtaaa
    24841 aagaaacaca gtcagtcatt atttgaacca gttaaatgat gagcaactac tagtcaaaat
    24901 caattctcga ccagtgattt atttccacaa agaagctttt gaaaaacaat tttttaagtt
    24961 acgcacgaat ttttattttt ctatcgatga tctcaaagag gaacagcctt ttttcgcgca
    25021 accaaaagac ttattctctt ttatgatcgg atacgacgcc agtttgaaag aaagtatcga
    25081 acaaatcaaa acagctcttt actatccaga tggcggatta ccacttttga ttacaggcga
    25141 aagtggcaca ggtaaaagtt atttagttaa tttggtctat cagtattgtc tgcttcatga
    25201 tctgctagaa gattctgcac cattcatcac ttttaattgt gcacaatatg cagataatcc
    25261 tgaacttttg actagcaatt tgtttggaca tgttaaggga gcgtacactg gtgcagaaga
    25321 aaataaaaaa ggggcttttg aagcagcaga tggtgggatc ttgtttttag acgaggtaca
    25381 tcgcttaaca ccagaaggtc aggaaaaact gttcacctat cttgatcaag gtgtgattta
    25441 tcgtatggga gaacctaatt tgtcacgccg gatcaaaaca cgtctctttt ttgctacgac
    25501 tgaagagatt actagtcatt tcttaacaac tttcattcga agaataccta tccgaatcga
    25561 actacctgca cttaatcagc gtagtcgtaa tgaacggcta gaactgattt actctttttt
    25621 tctcgaagaa caaagaaaaa tggggagccc tttgtcagtc agtggaccag ctttgtcact
    25681 tttaacaagt cagaaattat ccggaaacat aggagagctg aaaaatatcg tcaaagtttc
    25741 agcagcaaaa gcatatgctg aacagcgtga acagcctgaa atccatgtca cgatccatca
    25801 tttgcaaaaa gaactacttg cgcagcctat tactaaaaat acagcacagc aaggaatcta
    25861 tattacgcaa gaccagacct tagagcaatt aatggagaag caacagcccg aacagcagcg
    25921 aatcgttaaa agttttgagc aaatcctaat tgatttcaag aaaaataact gtctgctata
    25981 ttcttgcgaa ggaaaattaa agcaagaagt gattcaatta tttgattttt tgctgtttga
    26041 gaccagtaga caagaaaaac acgagctgct ggtttattgg acacaatcta tccgagagac
    26101 ctttcgacaa atggaaactg cctatcaaat caaattcgat ggaaacagtg tctatgcctt
    26161 gagttactat ctctatcagc gaagcactat caaatggtta ccggaagaac atgatattat
    26221 ccaactaatc aaagaattag aacaacaagt ttctcagtct tatccatcta gttatcacta
    26281 tgtccaacga attctggaat taagtaagat caaattagat attgaagaca caagtatgga
    26341 tcggatttta ctgacgatct atctaaaaaa agcgaaatgg tcaaaagaaa aacgactgcc
    26401 aaaagcgatg attgctgctc acggttatgc aactgctagc agtatggcaa atgtagtaaa
    26461 tcgtttatta caagaggatc tatttgaatc atttgatatg ccactagatg tcacaccaca
    26521 gcagattgct tcagaaatcc ttgattattg cgaaaacagc gatgtttcaa atggattgat
    26581 catattagta gacatgggat ctctgaaaga aatccatcag ttttttaaaa aacagttatc
    26641 cgtaccgctt ttgattttga ataatgtgac gacaccttta gcaatcacag taggggaatg
    26701 cctgcaaaaa aatgtatcat tagaagagat agcagaagag actgttcgac agatccagcc
    26761 agaatggcga ctgctctatc cagaggaaaa caaacccaaa gcactcatta ctacttgctt
    26821 cactggaatc ggtacggcgg tccacttaag tgaactgcta gaaaaaagct tacctacggc
    26881 ttgtcagttg aaaatcatcc catatgaata tcagcaatta aaagataaga aaaacagtga
    26941 cccattattt tccatatacg aagtcgtagg aatgattggc accacagatc ctgatatcac
    27001 caatatcgac tatctttcat tagaagacct catctcgggg gaaaagatgt ctgtactagc
    27061 tgaatggttg gacactacta tgaatgcttc agagaaggaa atattcaatc agcgcatcat
    27121 tcgtaatttt tcattggaaa aagtactgga ttccgtgact attctagata cagagaaggt
    27181 gatgggagaa atcgatcatt ttatgagaga attagaggtg cagttgaatc gaagcatcag
    27241 caatcccaaa aaattagcgt tgtatgtcca tgtgagctgt ctcattgaac gattgatccg
    27301 acaaatgcca atcgaaacct atcaaggatt agagaaatgg aagcagtgtc aaaaagaagg
    27361 gttatctgct attaaatctg cgtttagtgt cattgaagag cattatagtg tcatgatccc
    27421 caattctgag attgcctata tctatgatat tttgagtgaa gatacagagt ttctctcgat
    27481 agaagaagaa ttttgaacat aagcaagttg ttgccactaa ttttggcatg caacttgctt
    27541 tttttagtat gtgtaagtga aaagaggaga tgaaaaagtg gatagagaaa ttatcttagc
    27601 ttcccatgga aaatttgctt cagggatatt agattcatta gagctgatct atgggaaaaa
    27661 tcattctatt actgtattcg attgctatac tgatgagaat tttgatttag cggaaacagt
    27721 aaggcagctt tttatgaggt acaaagataa agaaatcatt gttctgacgg atctttttgg
    27781 cggaagtgtc aacaacgaat tcttacaata tatcaatcgg gatcatgtct atctcgttgc
    27841 cggattgaat ttgccactat tgattgagct agtatctcgg ttggacgctg aagtagagac
    27901 aactgttttg atccagcagg tgctaactga ttcaaaaaaa atgatccagt tttgtaacga
    27961 gcggctcaat cagaaaatag aagaagacga attttaggag gaagaaaaat gattgtattg
    28021 acaagagtcg accatcggtt attacacgga caagtagctt tttcatggac acaaacaata
    28081 ggcgcagact gtattttgat tgctaatgat gatgtaccta cgaatgagat acgaaagaca
    28141 acgatcaaat tagccaagcc acaaggagtc aaactagtga tcaaatcaat tgatgactcg
    28201 attgccgcgt tgaaaagcgg ggtgacagat aaatataagt tattcatcgt agtggaatcg
    28261 atcgaggatg catataagct ggcagaagcg tatccagaaa tcaaaaccat caacttagga
    28321 gggatcaagg caaaacctgg tactcgcagc atcggaaaag cggtaaatat tttaccagaa
    28381 gaggaggaac gcttgaaacg attaattgac aaacaaatag aagtagaaat ccgtcaagta
    28441 ccagcagata aaaaagtgga tgtaagagaa gttttgtaag aaaggaagaa cctcaatgat
    28501 tgtacaagca gtcttattag gaattgttgc atttgtcgca caatcagaat atgcactagg
    28561 gacatcgttg ctttctcgtc cgattgtgac ggggctgttt acaggaatcg tgttaggtga
    28621 tgtaaaaact gggatcatta tgggcgcaac tttagagtta gcatttatcg gatcgttctc
    28681 agtaggtgct tcgattccac ctgatgttgt gaccggtggg attttaggga cggcttttgc
    28741 tattacagct ggagccggta cagaaaccgc gcttttacta ggtttgccga ttgctacgtt
    28801 gacattgata ttgaaaaata tttacttagg gcttttgatt ccaattatga accataaagc
    28861 agacaattat gcagaagaag gaaattataa aggagtcgag catatgcact tgcttgccgg
    28921 tatcggtctt tccttgatgc ttggtgttgt ggtaatggtt tcttatatgg tgggaagcaa
    28981 tacgatcgga aatctattaa atatgattcc tgacttcgtt caaaaggggt tgtcagtagc
    29041 tacagggttg attccagctc ttggttttgc gatgcttgct cgtttgctga tcaacaaaca
    29101 agttgcacca tattttttct taggttttgc tatagcttct tacttagaga ttcctgtgac
    29161 cggagtagct atttttggtg cgattttagc agttgtggta gtcaatatta tgaatgtccg
    29221 tcaactacca cttcaaacaa ctgcagggga ggtagaagaa gatgaagaat tctgagatca
    29281 aaaacagcaa aataacgaaa aaagagctaa atcacgtatt ttggcgttct tttcaaatgg
    29341 agttttcttg gaattatgaa cgtcagatga atatggctta tgtttatgca atgattccga
    29401 ttttgaaaaa gttatatcag tcgaaagaag aaatggcagc agctttgaaa cggcatctag
    29461 agttttttaa cacgacccca catatcgtta cactgatttt aggaatcacg gctgcaatgg
    29521 aggaagaaaa taaggatgat cctgaatttg atgtgactgc gatcgatagt atcaaaacat
    29581 ctttgatggg acctcttgca gggttaggag attctttctt ttggggaact ttgcgattga
    29641 ttgccacagg tgtgggtacc tctttagcca tgcaaggaaa tattttaggc cctatcttgt
    29701 tcattttgat tttcaatatt ccgcacattc tgtttcgcta tctagcaaca ggttggggat
    29761 atcgcttagg aacgggtttt ttgaaaaaga tccaggcaaa cggtatgatg gaaagtttga
    29821 cattaggcgc gtctattatt ggactgatgg tcgttggagc aatgactgcc acgatgatca
    29881 ccatcaatat tcctttaaaa atcggttcag gagaaaatgc tgtgactgtt caaagtattt
    29941 ttgatgatat cgttccaaat atcttatgtc ttggagcatt tggcatagtg ttttatctat
    30001 tgaagaaaga agtgaaacca ttaacgatct tgatcggact agctttcttt ggaatttttg
    30061 gttcgtggat agggattttt taaaaaagga gcagagaaaa tgtttacgat gcaagattat
    30121 atctatgaag aaaaggaagt actaagtacg atcttgaaaa agaatgattt ttcgacaaga
    30181 gaacatatga aaaaaacagt gaatttactg atcttagcaa cgggttcatc ttataacgct
    30241 tgtttagcag cgaagccagc attggaatct tatggtgatc ttactgtcga tattcaagaa
    30301 cctttttatt ttttcaatta tggaaaactt tctccttcaa tcgatactgt gattgctgtg
    30361 tcacaaagcg ggaaaagtgc ttctactgtt gaagcagtaa aaatgataca aaaacaaggg
    30421 ttacctgttg ttgctatcac aaatgacgtg caaagtccac ttgctttaga agctgcacaa
    30481 attattgatt taggtgccgg ggtagaatca gtaggcttcg ttacaaaagg ctattctgcg
    30541 actgttttac agttactact cttagggata ggaattggaa taagtaaaaa taaaatcagc
    30601 aaaaaaatag aacaagacta catgcaacag ttgcgcaaaa taatcaacca tttaccagcg
    30661 attattcaaa aaacggaaga attttttgat ggatatcaaa gtttatttcg tttagcacag
    30721 cgtttcgtca ccattggata tggtccaaac tggggaacag ctaaagaagc agagacgaaa
    30781 ttgactgaaa caatccgcgt tccgtcacaa ggatttgagt tagaagctta tatgcacggc
    30841 ccatacctgg aggcagatgc ttcgcatttg ctattcttta ttgaagggga aagtgtaaat
    30901 aaggaacgtt cgcaaaagtt gcaaagatat atgagtcggt acgttggaga gactctcacc
    30961 attaccacaa aaaaagcaag aaacgagaaa acacttggat tagcaataga atgtgatgaa
    31021 tatctgtctg tattagcgct tgtcgttccg tttcaactat tcgcttataa aaccgcagta
    31081 gctaaaggta ttgacttgaa caaaaagatt tttgaagact ttgatacagt cttgaaaagt
    31141 aaattataag aggtggaaat catgctgaaa ttcaatgaag aagaacaaat caaagcaata
    31201 aaaggcgcac tagctttacg tccgcaagta gaagaaatca tcgataaact ttatgcggaa
    31261 aaatttgacg ctgtctatta tctaggaatc ggtggaacgt atgcttcctc gatgcaagct
    31321 gtcacttata tgaatgggaa aagcaacttg cctgtctttg tacagcatgc tgcagaatat
    31381 tacactactg gaaacaaaag attaacaaaa gattctatcg ttattttgtc ctctgttacc
    31441 ggtacaactc aagaagtagt gaaagcagta gaacaaatca agaaagtagg agcgacactg
    31501 attggattta tcgataaagc gaacagtaag ttgtctcagc tatgcgattt tgtggtcact
    31561 tatccagctc ctggaacaga acaaatcaaa ttttttatgg cagcagatcg attgatgtat
    31621 ttgaatggag aattcgaagc ttattcagaa tactatgaac agctcgaacg ttatttacca
    31681 acagggttag tggaagcaga gaaaaaagca gatgcatttg gcttatcatt tgcagaaaaa
    31741 catcgacgtg atagtatgca ttattttatt ggagcaggga atcaatgggg agctgtttat
    31801 tcatatgcca tgtgttactg ggaagaacaa agctggcttc catcgaaatc tatccatgct
    31861 gccgagtttc ttcacgggac gttagagatc gtagaagaaa caaccccagt cacattattt
    31921 ttaggcgaag atgaacagag agtgttatct gaacgtgtgg ctaaactttt gccaaaaatt
    31981 tgcagcaatt atacattgat agacacaaaa gactatccag tagaaggtat cagtgaaaaa
    32041 tatcgcggac gtgttttgtc tttcttattg atgcatgtag tgacacaacg catcgatgct
    32101 catgtggagc agttgaactg ccatccatta gagatacgcc gctactatcg tcagtttgat
    32161 tattaggata gactggataa aaagaatata aaaaataaag agagcagcga taactgctcc
    32221 ctttattttt ttaaaatatt ggcatcaatt acgttataaa acacattttt tgcattatct
    32281 acagttcaac ttataatctg tttaatggga catgtgggtg ccaaccattt tttgttatgc
    32341 aatagtacta tttagttgtt gcatggatcc gtagtaaaag tccaagtaca acgagttgcg
    32401 ccagtttaca tagcgttttg tatccagaat gttgactaaa acaaaagttc ctatgtcaac
    32461 cgtcaacaga gacaatctgt ccgttgcagg acctgacaga tttagaaatg ttcaatctgt
    32521 ctcttatgta ttttaaatca atactcacta ggagaagttt cctaacctcc taatagtaat
    32581 gctggtagta acattgtcaa aactgcttcc tcttaggaac aagcaaagca taacgagtgt
    32641 aaaaataaaa tgtagactat tgtaacatat cattgattat ttcaagtaaa aacgataaaa
    32701 gaagtaaaca aacacttaaa aacaatggaa attagtccca actttaagaa atgtatacta
    32761 taatggaaat tatcaattat ttatttggag gaatatccat gaaacaaaaa atcgcagttc
    32821 ttggtcctgg atcttggggc actgctttag cacaaacatt ggcagaaaac gggcatgatg
    32881 tccgtatttg gggaaatgta cctgaacaga ttgatgaaat caataagtat catacaaatc
    32941 agcatttttt acctgatttg acgatcccag aatcgattat cggatataaa gaattggcag
    33001 aagcagtgga taacgcagat gctattttat ttgttgttcc aacaaaagcg attcgttcag
    33061 tcgcacagga acttattggt aaaatgaata ctaagccaat cattatacat gcaagtaaag
    33121 gactagaaca agatacgcat aaacgaatct cagaagtttt ggctgaagaa attccagaag
    33181 aaaaaagaca ggcaattgtt gtattatctg gtcctagcca tgctgaggaa gtggcagtac
    33241 atgatatcac aacaattaca gctgcaagta ttgatcaaaa agcagcagcc tatgtacaaa
    33301 aactgttcat gaatgattat ttcaggattt atacaaataa tgatgtgatt ggcgtagaaa
    33361 ctggtgcagc actgaaaaat atcattgcaa ttggtgccgg cgctatccat ggattaggat
    33421 tcggagatga tgcaaaagca gcaatcatga ctcgaggatt ggcagaaatc agccgtcttg
    33481 gtgtcgcgat gggtgccaat cctctgacat ttatcggttt gagtggtgtg ggggacttga
    33541 tcgttacttg tacaagtgtt cattctcgca attggcgtgc cggtaacttg ttaggacagg
    33601 gacataagct ggaagaagtt ctagaaaaca tgggaatggt cgttgagggt gtggcaacga
    33661 caaaagcagc agtagagcta gctcaacagc taggtgttga gatgccaatt actcaaacaa
    33721 tctataatgt tttgtataat ggagaagata tcaaaaaagc agcaaaagaa atcatgttac
    33781 gtgacggaaa aatggagaat gaattttctc ttcgttaaat agatgatcaa tagttaatc
//
LOCUS       DACDYI010000025        32946 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.025, whole genome shotgun sequence.
ACCESSION   DACDYI010000025 DACDYI010000000
VERSION     DACDYI010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.025"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            471..2108
                     /locus_tag="IWM52_001160"
     CDS             471..2108
                     /locus_tag="IWM52_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP9973425.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(2253..2993)
                     /gene="truA"
                     /locus_tag="IWM52_001161"
     CDS             complement(2253..2993)
                     /gene="truA"
                     /locus_tag="IWM52_001161"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP9973426.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(3184..3819)
                     /locus_tag="IWM52_001162"
     CDS             complement(3184..3819)
                     /locus_tag="IWM52_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP9973427.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3966..5207)
                     /gene="sstT"
                     /locus_tag="IWM52_001163"
     CDS             complement(3966..5207)
                     /gene="sstT"
                     /locus_tag="IWM52_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAP9973428.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            5405..6403
                     /locus_tag="IWM52_001164"
     CDS             5405..6403
                     /locus_tag="IWM52_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973429.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            6416..8434
                     /locus_tag="IWM52_001165"
     CDS             6416..8434
                     /locus_tag="IWM52_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAP9973430.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(8494..9210)
                     /locus_tag="IWM52_001166"
     CDS             complement(8494..9210)
                     /locus_tag="IWM52_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP9973431.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(9437..9979)
                     /locus_tag="IWM52_001167"
     CDS             complement(9437..9979)
                     /locus_tag="IWM52_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9973432.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(9984..10211)
                     /locus_tag="IWM52_001168"
     CDS             complement(9984..10211)
                     /locus_tag="IWM52_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAP9973433.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(10290..12170)
                     /locus_tag="IWM52_001169"
     CDS             complement(10290..12170)
                     /locus_tag="IWM52_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP9973434.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(12359..12973)
                     /locus_tag="IWM52_001170"
     CDS             complement(12359..12973)
                     /locus_tag="IWM52_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9973435.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            13115..13675
                     /locus_tag="IWM52_001171"
     CDS             13115..13675
                     /locus_tag="IWM52_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9973436.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(13980..14294)
                     /locus_tag="IWM52_001172"
                     /pseudo
     CDS             complement(13980..14294)
                     /locus_tag="IWM52_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(14548..16183)
                     /locus_tag="IWM52_001173"
                     /pseudo
     CDS             complement(14548..16183)
                     /locus_tag="IWM52_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16738..17301)
                     /locus_tag="IWM52_001174"
     CDS             complement(16738..17301)
                     /locus_tag="IWM52_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP9973437.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(17316..18359)
                     /locus_tag="IWM52_001175"
     CDS             complement(17316..18359)
                     /locus_tag="IWM52_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973438.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(18492..18896)
                     /locus_tag="IWM52_001176"
     CDS             complement(18492..18896)
                     /locus_tag="IWM52_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP9973439.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(19129..19764)
                     /locus_tag="IWM52_001177"
     CDS             complement(19129..19764)
                     /locus_tag="IWM52_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP9973440.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(20030..21082)
                     /locus_tag="IWM52_001178"
     CDS             complement(20030..21082)
                     /locus_tag="IWM52_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9973441.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(21095..22033)
                     /locus_tag="IWM52_001179"
     CDS             complement(21095..22033)
                     /locus_tag="IWM52_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP9973442.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(22484..23134)
                     /locus_tag="IWM52_001180"
     CDS             complement(22484..23134)
                     /locus_tag="IWM52_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973443.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(23234..23743)
                     /locus_tag="IWM52_001181"
     CDS             complement(23234..23743)
                     /locus_tag="IWM52_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAP9973444.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(23944..24813)
                     /locus_tag="IWM52_001182"
     CDS             complement(23944..24813)
                     /locus_tag="IWM52_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9973445.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(24833..25954)
                     /locus_tag="IWM52_001183"
     CDS             complement(24833..25954)
                     /locus_tag="IWM52_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP9973446.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(25956..26351)
                     /locus_tag="IWM52_001184"
     CDS             complement(25956..26351)
                     /locus_tag="IWM52_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP9973447.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(26370..27167)
                     /locus_tag="IWM52_001185"
     CDS             complement(26370..27167)
                     /locus_tag="IWM52_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9973448.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(27169..27957)
                     /locus_tag="IWM52_001186"
     CDS             complement(27169..27957)
                     /locus_tag="IWM52_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9973449.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(27971..28447)
                     /locus_tag="IWM52_001187"
     CDS             complement(27971..28447)
                     /locus_tag="IWM52_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9973450.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(28519..31176)
                     /locus_tag="IWM52_001188"
     CDS             complement(28519..31176)
                     /locus_tag="IWM52_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9973451.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKK
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNISKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDA
                     IYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFK
                     QSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
     gene            complement(31195..31599)
                     /locus_tag="IWM52_001189"
     CDS             complement(31195..31599)
                     /locus_tag="IWM52_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HAP9973452.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            complement(31831..32088)
                     /locus_tag="IWM52_001190"
     CDS             complement(31831..32088)
                     /locus_tag="IWM52_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HAP9973453.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
     gene            complement(32213..32545)
                     /locus_tag="IWM52_001191"
     CDS             complement(32213..32545)
                     /locus_tag="IWM52_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973454.1"
                     /translation="MPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGLDLKNL
                     LCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDLLIRWQVITNFLMELKKY
                     SNTQTKFS"
BASE COUNT        10411 a         6498 c         4969 g        11068 t
ORIGIN      
        1 aactaatcag aatcgtacaa atatttaata accaaatata tagaacataa cttaatatta
       61 agttatttga aattccataa aaaacgactg caggaattat tgtaagaaaa ggataaaaat
      121 agttcagtcc atctccataa ttgttaaatg ttgtatagtt gattggccct tcaaaaatag
      181 aagataaccc tttgattcgt gaaagatgaa aagataaatc acctgttgta taaatttctt
      241 gagcatttct cataaaattt actgaatata aagtactaag taagataaga actactaaaa
      301 gaaatgcttt tttacttatt ttcatagatt tgttgttctc cttttttaat tctattttca
      361 gtatactaga attgtctttt tttaataagt tttgcgttaa atttttcata gcactagtat
      421 gatatactag aaaagaatca atttttatta cttttaaata ggagaaaaaa atgaaaaaaa
      481 acaaaaacca aataatagat atcattttta tgttatttct aagttttgct tacgtcatcc
      541 ctctattcat gtctaaggga atctatcata gtgtcaatca ggatacttac tttcatttat
      601 ctcgaatcat aggactagat aatgtatgga gttctccagt aaacttcaat aacttcgatc
      661 atcatggaac aatgatgaat attttttatc catggcttac tctttatcca gcttttttat
      721 tttataaaat gatgggaaat cttgttttaa gttataatat ctactatttt tttatcactt
      781 ttttaacaat ggtcgtttct tatttttcta tgaaacaaat aaaaaacaat cgttatattt
      841 cgctgttatt ttcgattatt tatacatttt ccgcctatcg tgccattgac atattccatc
      901 gtgcttcatt gggagaagct gtcgctttaa catttttgcc tttgatacta atggggtgtt
      961 acgaaattta tattagagat taccaaaaat ggtactggtt gtctattggc atgacgctag
     1021 tagtgtatac tcatttactt tctgtcgcta tggtttctgt atttattgga ggaacgttgt
     1081 ttctttcttt ttatttttgg gaccaaaaaa tcgctagact cttaagtctt ttaaaagcta
     1141 ccgtcctcac ctttttttta agtgcgggat tcttaattcc ttttattcaa caatctagag
     1201 cacaagaatt gaaagtccca ctgggaaaag aattaagtgg tatggctcct agtgatatgc
     1261 tgacacatat tttaaataat aactataaca actatactat tggattattc ttatttctcg
     1321 gattgattgg tgcttttatt tttataaaaa aattaactgc tgatgattta tttattttct
     1381 ttcttggtgt atttgttctt ttttgttcaa ccaatttatt tccttggcaa ctttttaacc
     1441 atacaccagt taagagtcta caatttgtct ggcggttaaa tggtttttct tctttattta
     1501 tagcctatac gatgagtata gtgatctatt atattttttc tactaaaaat aattcatgga
     1561 aaataatggt ctttacgttt cttgcactaa tattgcatct ctcaggagta agcaattcta
     1621 tccatgctaa caaagattca ttaacactta tagcacctga agacgctgtt gctatagctg
     1681 aaaattacaa tcatactgat tatgcaaaca aagagtcaat ctatcatcct gatatgatta
     1741 ataatgatca atatttacta ggtaaccagg aaataaatcc aacaactcat tttatgagta
     1801 acaaacttac aattgaatta gataattcga ataataaaaa tactgtcttg acgacaccta
     1861 tttatcgtta taaagggcaa gtggcttcta ttaatggcaa actagtacaa acaaaattat
     1921 ctaagtttgg cacaactgaa ttaactattc ctccaggtat aaataaagtc gttattactt
     1981 atcagtacac aaaactagca attgcttcaa gatatttatc tatcgttact ttgattttat
     2041 ttttacttta tcgatttact ttttctaaac aaaaacaacc gagaagagaa gtacaacatt
     2101 ctcactagat ttttatttaa gaaagacttt ttagctgtac acataaacag gctaaaaagt
     2161 ctttttttat atttctttaa tttgcttcat acattattta acatcgatat ttatactgtg
     2221 aaacatgcgt ttcatccatt atcaaagtat ttttatcgat aataaacttc atccaaaaac
     2281 agaccttgtg cagggacggt ttctcctgca tgttgccgca ctttgctttc aaatatttca
     2341 tcgattgttt ctatcggcat ggcacctgtg ccgatttcta gtaatgtccc catgataatc
     2401 cgcaccattt tatataagaa tccgtctccg acaaaagtaa aatgaagcat ttctcccttt
     2461 cgttcgatag ataattcatc aatcgtacgc actgtggatt tttttgattt tttcaatgca
     2521 gaaaaaccaa tgaaatcatg tgtacccact aatttttcgc aagctgcatt cagcttgtcc
     2581 atatccaact cttgagggaa ataaaagctg tggtttcgct caaatgctga aggaatcacg
     2641 ttgttccaaa cataatagct gtattttttg cccaccgcat tatatcttga atggaagcgc
     2701 tcaggcattt cttcgattct tttgatgaca atatcccgcg gaaggtaacg attcataaag
     2761 tcgatcattt ctgccagtcc aatttctgtg tttgttttaa aatttgctac ttgtcctcgc
     2821 gcatgggtgc cagcatctgt tcttcctgat ccaataattt ctatctcttc atttgtcatc
     2881 tgatgcaata ctttttcgat ttttccttga atggttttat cgtcatctcc aagtctttgc
     2941 catcctgaat atctttttcc gtcatattcg attgtcaact tgatgtttct catattttag
     3001 tggctcctct gtcttccatt tggtcttctc tctacctttt taagattgta gcacaaaata
     3061 tagctccttg cttctctaag acagaagcta tacaagaaaa ctgtggaaat atatatttca
     3121 gaaaaacaaa aaacagccac cggatgaagt atccgctgac tgttcaattt gacggctcaa
     3181 gttttactgt ttgagatgca agatactcaa ccaggcaatg ggcacataat taaagaaaaa
     3241 actgattctt tggcaaaaac ctattttatt cagtaatggc caagaattta tagaaggaat
     3301 tcttgccagt ccataaatcg ccgcaaaaag caaactgatc acagacagtc caaggtataa
     3361 gtggcttacg tccatttgcc cattttttcg atagatcaag aaaagaagaa ctggaaacaa
     3421 taaaaaacca ctgtaaccta aacctgatcc gatattatgg acccatgtcg agacattcca
     3481 cgaagattca tgggtattga tactgaacaa acctgtaaag atacaatcac ctataccata
     3541 gaacgcgatg gctgctgtaa ctagggtagc taacacacga gatgtactaa caaatgcagc
     3601 atacaatgca ggcaatgaca ggatgaaaaa gatccctgaa accacagacc aaataagaaa
     3661 cgcttttctt acaggactgg aaacctctcc aaatacactg atcacgctct tcatttgatc
     3721 taattcagga taatacaatc ctaatacgta cggagtcaaa aaatcactca gcacgcctaa
     3781 taataaaaag aaaaaaccaa atttacgtaa aaaatccatg atacgcctcc aataacaatt
     3841 acctatgctt ttaattgtta ttataaacga ctttatcttg atttaacaga aacctctatt
     3901 tacacgaaca tctatataac tcattaaaaa ctcgaatcat tttaaaaata ctaaatatct
     3961 aacgttcaac gttcacttaa tccatctaaa caatctttca atttgatttt ttccccactt
     4021 cttttacgcg cgcctagctc tcctattgca gtaaacaaca gatcacttga cgaatttaag
     4081 gcagtttcca ttgaatcctg gataacaccg ataataaatc caataccgac gatttgcatc
     4141 gcgacatcat tagagatacc aaacaaacta catgctaatg gaatcaataa cagtgaaccg
     4201 cccgcaacac ctgaagctcc acaagcagct aaagcagaca aaatacataa aaggactttc
     4261 aaaacaaaag gtgcttcaat ccctaaagaa tgtacagcag tcaacgtcat caaggaaatg
     4321 gtaatcgcgg caccgcccat attgatcgta gcacctaacg ggatcgatat ggaataagac
     4381 tcttttgtca atttcaatct ttcagccaac atcatgttta tcgggatatt tgcggcagaa
     4441 cttcgggtaa agaaagcggg aagtgcgctt tctttcaaac aaaagaaaat caatggatag
     4501 ggattttctt taataaacaa aaacgtgagg aatgggtaca caactaatgc gacaaacagc
     4561 atcgtaccga tcaaaacaag taaaagctgc agatactctg ccaatccagc gattccagtc
     4621 gtagcaattg attgataaac caatcctaaa atccctatcg gagcaaatgc aataatcact
     4681 tgtacgactt gtgacaacgc tatagatatc tgatccacca actcttttgt cgattctgaa
     4741 cgtgctcgaa aagctagtcc caacaataca gcccagaata agacgccaag gtaattcgca
     4801 tcaataagag attgaacagg attttgtgtg acattcgtca acatagtaga taaaacactt
     4861 tccaaacttt gcggtgcttt ttcagatact gtttcttgta aagtaatagg aacagtaaaa
     4921 attgaactta caataactgc tacaagtgca gaaagcaatg ttcccactaa atataagatc
     4981 aaaataggct tcacgaatgt cttattccca attttatgct tggcaattga cgccatcgtc
     5041 aagaaaaaaa ccagaatagg tgcgattgcc ttcaagcaac tgacaaacaa cgttcccaat
     5101 attgaaataa atgtccatgt ggggaaaaag ataccaaaca atattccgac taaaatacca
     5161 acaacaatac gttgaactaa cgacatatcc aaaaacttct ctaacaaatg accacccttt
     5221 cacaatttta acaatcattg tatcatttta tgcaaagaaa ctaaaggtat aattttctaa
     5281 aaattctaac aatacaaaca accatttctt gctaattttg tactttttct gtctacacgt
     5341 tgttttttga gatattctaa gttaggatgt tattaatggg caacttatca ggaaacgttt
     5401 tcttatgttt ttgtccaatt tttttgtgag gtgtttattc ttgaaaaaaa taaagaagga
     5461 gagttatggc ttcctgacaa tctttgtcgc ttgtataatg atggttttac agcgtatatt
     5521 gatttcagca gtcactattc ctacatatgc aacaaaccct ggtcgattct acatctatac
     5581 atttttacaa acttccgttg ttattggtgc aaatttgatc ccactttaca tgggttacca
     5641 atatcaaaaa atcgcccgtt taaaagtttt acattattta agtcgttttg cctttatatt
     5701 cctaatagcc acactagttt gcaatatctt tttttacgta caagctggca gcttgaatat
     5761 tcgtgattat tggcttatcc taacacctat cagtcagaat tactttactt atgctgtttc
     5821 ttgtactttg cttttttgta gtattcctta tacagttgga tttttaaata aattatcgaa
     5881 aacaacaatt aaatatctac tattattttt aacaattgga ttagttggct gctctactct
     5941 atttaataaa gacccgtttg cattacagaa tggacagagt attttatgga ttttttattt
     6001 atttttaatg ggttatggtt tgaaagaatg gaactggaaa gaaaaggtcc gctataagct
     6061 tcctcaagta tttatcagct tgattgtttt attcggtttg attattttga tgacacaaat
     6121 ttcactcatc atcagaggaa atacagatac agcgctgagg ttttctaaac catattctct
     6181 cttcgcaatg tattatactt tctcttcgtt tttattttta gaaatgctat ctgataaatt
     6241 aggtataaaa ggaagaggaa attttcttgc tgtatttttg attgtcactc aagttatatg
     6301 taatttccct ttagtttctt atcgcgtatc tacatttatg aaacgtgaat ttccaaactc
     6361 agggaaggca tggctgataa atatcgggca gttttttatt tgatatttat tagcagtgtc
     6421 cctcctagtt atctttttat tttatataca aaaaatccgt ttcattcatc gtttcatcga
     6481 atacttcgct tttgattcga ttgaacaatt aactcagcgt tggaaaaaaa gaattcattg
     6541 gctttttgtt cataaaagtt attttttagt tgcagcattt ttttactgct ttaccttcgt
     6601 gcagattttc gttttagaac caaaagaagg ttggaaagaa actatccagg ttgccttaaa
     6661 aatatttact cagcgacaag cacccatgat cctgacaatc atcattatta tgggattttt
     6721 cttcttacta ttgttgattt ccaatcgttt ttggtacgcc ttgactgcta ccttgatcat
     6781 taacttactg ctcactattt cgacagtaat caaaatggaa atgcgggaag agcctgtctt
     6841 tccttcagat ttaaaaatgc tgaccggatt gtcagaacta ttatcgatgg ttagcccagt
     6901 cttattaatc gtgggcggat tgatccttct cctattattg attactagta ttattgtaca
     6961 acgtagacta caaaaacagt attccttgaa gattcattgg aaacgtcgat ttatcagtat
     7021 cgctgtatta ttaggatgtt tttcgggtgt ctttttcatt aatcacaaaa attcgccttc
     7081 tttcttgctg tttaatttat ttaaagtaaa caagactttc tttaaccagc aaggcgccgt
     7141 aaaagataac ggaccagtca ttcaattcct aaacaatatc gacattaaaa tcatggaaga
     7201 acctgctggc tattcgaaag aaaaaattaa tggaataatg aaaaagtaca acacaaaggc
     7261 agatgaaatc aaccaaacaa gaagtgactg gttaaacaat cagacgatcg ttctcaattt
     7321 aagtgaaagc ttcagtgatc ctgcacgtgt ccctaatctg acagtaccta cagaccctat
     7381 tccttacatc aaaagcatta taacagaagg aacaggagga ttgatgttat ccgtcggtta
     7441 tggtggcgga acagctaaca tggaatggga aggactgaca ggcttgagta tcagtaacct
     7501 gtctgcttcg ttagttacac cttataccca gctagtggag cgtcagaaaa taagtccaaa
     7561 tattaccgat ctattcgatg aaaaaatagc catccatcct tttacagctg ctctttatcg
     7621 cagaaaacaa gtgtttgata aatttggttt tgacaggttc tattatttag acagtccgga
     7681 taaattaact tatacggata aaatagaaca aagcccacgc atatcggatg attcagcata
     7741 caaagaaacc ttgaaagctt tacaaagcaa tacggaaacg tcacaattca tccaattatc
     7801 cactatgcaa aaccatatgc cttatgatga ttactatagt gaattgaact acactgctga
     7861 aggaactgct gtcattccta atagaagaaa agaattacaa acttatatgc aaggccttca
     7921 ctataccgat acagcagtaa aaaaattcat ccaagaaatc gacaaaatcg ataaaccgat
     7981 tacctttgtt ttttatggtg accatttacc cgcaatctat agtgggaatg attcgaaaaa
     8041 atatggatta gaacagcacc agacagatta tttcatttat aacaacaagt atagtcgtga
     8101 acaagcagct acgttaaata aagaagttgt tgctcctaat aactttggtg ctatggctct
     8161 tgagcatgcg aatatcaaag taactcctta ctatgcgttg ttgacagaag tatctaatca
     8221 tctacctgca ttaacaattg atcctacaga aagcttgtct aatcgtttta atggaaaaca
     8281 ggtctttgtt acagaggata gtcaaattat ccaagaaaaa aatttaactg agaaacaaaa
     8341 agaaattcta gaagattatc gtctgataca atatgatctt gttgcaggta accagtacag
     8401 tgcaacatgg gcgacacaaa aaataaaaga ctaacaaata aactgagcaa ggggttgtga
     8461 taaaagacat gtcacaaccc cttgtttatc tcattaattt gcttgtccat aaacgatttg
     8521 attaaagcca tttttcaatt cattattcgc tgtctcgttg aagtaaagat aattatcggg
     8581 attcccctga ttttgttgtg tcaccaatac ttttggctga ttttgctgaa acccgaatag
     8641 atagacgtgc tttttcagat atggttctgt ctctgcatcg gaatagacgg ctgtgatttg
     8701 atagtctgct tgacctgtcc cactttctga ccattctgca gaaacaggca ctccaccaat
     8761 agccatcttc cactccccgt tcaaaatagc ttgagggact ttaagaccat ataagtccac
     8821 actcatttga ttcgtataac ttttgtattg ttgtcccatt gtttggcccc atttggtcat
     8881 aaatgcctct aattgtgcgg atttgtctgc attccacagt gtttgttcaa cttgttcgct
     8941 gctttctgaa gaggaagatg aagcctctac agaagaactt gaagtagatg tttgagttgt
     9001 ttcagtcgtt ttggaagact ctgacgaagt agaggtatct gctgtttctg tacttgctgc
     9061 agttgacttg ctctctgtgc tagttgttgt agtagttgca actgttgctt tcgctgtttc
     9121 ttttgttgat tcttttttcg attcattccc gttggtacaa ccagctaaca aaaaagctag
     9181 accaattgtt actaaaatcc tttttttcaa ttaaaaaact cctttcgatt actcccactt
     9241 ctttattcta tcaaataagc aatgtgattt tattgatttt tcgtattttc tcatcaatct
     9301 ttcaaatctt ctaaaaactg agaagcaccc gctgatgttg tctgtaatac atcagcgggt
     9361 gcttctttta ttttaatctt cgtgattttc caatttacct cacgctttat atttgtattt
     9421 gaagaaacat agacttttag tcatcatcgt cttcctcatc caaatcttgt cggacagttt
     9481 ttccttggag tctttgtact tggtagatga tttctttatt gaaacttaaa agttcaacaa
     9541 gcaattgttg caagccatat ttttcttctt tttcagctaa tttgattgcc cgtgtgataa
     9601 agacattttg gaaatcaaaa tcttttgcaa agttttccaa cattgttgag gctggtaaat
     9661 atttgttttg accatgttcg taaatttttc cataacgcat gacttcttct aaagtagacg
     9721 gaacgatttc attttcaatc actaactcat gagagatttt attgaaccat ctcgcttctt
     9781 gatcagcata ttctgaaaaa agggtactta attcttgcga agatgtaccc aaaacataca
     9841 attgtgcttg gatcaatttc atttcttcta atttaaaatt tgctaataca tgccccacca
     9901 tggcgccagc agttggttta tgatggtcga tatctgcttg ttttgtttct tgaacaaatt
     9961 tttcttccac tatattcatt gatttacgcc tcttttactt ttgctgattg gacttcataa
    10021 cctaattttt ccacaactgc agctaattgt tcagggtgga cttcatctgg atcaaagttt
    10081 gctttgattt tgctcgcatt aaacaaaact ttcgtattgc ttaccccagg ctgttgagaa
    10141 accgcatgtt cgattttttg catacagctt ggacaagcta gtgtatctaa ttgcataata
    10201 atttttttca tcgtagttct tccttccttt ttcttgatag tcttattcta gattacagct
    10261 tccataaaaa aattgattct tatcaagttt tacgattttt cttctttgct ttcgtgtcca
    10321 aaatacaata aacgcatcgc gttaaagata acgatcaaga tactagcttc gtgaacgaac
    10381 atcccactag ccatgtaaat aaacccggca aataagccgt tgagtaaaaa taagacgaca
    10441 ccaatggcaa tcactacatt ttcacgggta tttaaaacag ttttcttagc caagcggtag
    10501 gcatgaacca acgattcaaa attagattgc atcaagacaa cgtcactcgt ttcgatagca
    10561 acatccgttc ctgatcccat agcaatcccg atatcagccg cagccaaaga gggagaatca
    10621 ttgattccat ctccaataaa agcaacgcgc aatccttgtt cttggtattt tttcacaaat
    10681 tgaacctttt cgtctggtaa taattcagca tggacttcat caatgcctaa catatcagct
    10741 acataatcag cagtcatctg attatcacct gtcagcatca ctaactgttt tgctcctgct
    10801 tgtttcaact tagctaattg ttcagcgact tctggtcgaa tcacatctga cacacctatg
    10861 atttgagtga tttttttgtc gattgctaca ataatgaccg agctgcctgt ttgttgtaat
    10921 tgatgtaaat cttgtacttg ctttggtgtg agagaaagtt tgtgagcggc aatcaatttg
    10981 gtgttgcccg cacaaactgt atgaccatca atctcagctg tgattccttg tcccttgatt
    11041 gtctgattat ctttgactgc taattgttga aaatcaatgt ctttctcctg agcataatgg
    11101 atcacggcgc gccccagagg atgatcggat aacgtttcta atcgagccac taaagctaag
    11161 ctgtcccctt catcctctgt atagttcttg atagtggata cagcagtagt tcctttggtc
    11221 aatgtaccag ttttatcaaa gacaaaagta tcgattttgg aaaaagtatc cattgcttca
    11281 ccgcctttga ctaagatgcc ccgtttcgct ccattaccga ttcctgctac attcgaaaca
    11341 ggcgcaccaa tgactaaagc gccaggacaa ccaagaacca aaacagtgat tgctaaacga
    11401 aaatcacgac taatcagacc aacaaccaac gcaataagta aaacggttgg agtataatac
    11461 tgagaaaatc gatcaatgaa tttttccgca tgggatttgg tatcttgtgc ttcttctact
    11521 aattcgataa ttttggcaaa ggtcgtatca tctccgacct tagttgcttc aatcgtcacg
    11581 tagccatctg ctaaaatcgt tccagagaag acttcatctc cactgatttt gacgacttgt
    11641 cgtgattcac cagtcactgc ggcttcatcc gcataccctt tcccgtcaat gactcgtccg
    11701 tccacaggaa tagctccacc agtttttact aagacgatat cgccttcttc cacgtcatcg
    11761 atttccactt cttcagtcgt tccatcttct tggacaacac tagcagtaga tggggccatc
    11821 tcggtcaaag ccttgattga ctcccgcgtt ttggtcaatg ttttttgttc cagatagcta
    11881 ccaaacaaaa acaagaatgt aacgatagct gactccttgt attctccgat cataaaggca
    11941 ccaagcacag caatagtaac gagtaaatca attgagataa ctttgacttt cactgcctga
    12001 tacgcatgga tcataatagg tatgaccgca ataatagaag ctatggctag agcaatgtta
    12061 tagaacaaga caaaaccagt aaattttccg ataaaaccaa aaataatcaa cacagctgat
    12121 aaggcggtga tatgatttcg atgctcttgg atccactttt gtaatttcat ctttctccct
    12181 cttttccttt cattcttacg agactaagta tagatactta tccatcataa aaaattgacc
    12241 actatcaagt ttagtcaaaa aggcggtttt cttttggtag aatacactac aaaaaatagt
    12301 tagcaagaag actcactaaa agtgatcttg ccaactattg tcgtttctat cgaaattttc
    12361 attgaatgat tttcctgtac agcatacatc ggagatctat ttctttatct aagattttta
    12421 tcttgctaat tttttccttt agtctttcaa atccacgtcg ttcatagaat tgatacgtac
    12481 agccactatc tgtaaaaagg aatatttctt ttcctttctc tcttttttca aattctgtta
    12541 gcaacttggt accgatgcct tttgttttca aatcaggatt tgcagctaaa aaaatgatct
    12601 ctccatctgg cgaatgcttc tttaaatatg ctgcaagcat ttgttcattt gctgtctcgt
    12661 aagctccttt actttcttta gcaaatatat tttgtaaaaa atcgaatatc ttaacatata
    12721 tttgctgtcc aaaagaacga tgtttctttt tttctccttt tatctccgcc agcaaaacac
    12781 ctgccaactc gtcctctgca tacaaagcaa gaatttgagt tgctcgattc aactccatat
    12841 acaaaaaata tcttccgtaa agattcagca gaagttcatt ttccatatat ctctcaaagt
    12901 tcattccttc aacagcaaaa tagatagctt ttttgtaatc tttcttttct aagggtttaa
    12961 aagttagttt catcatttcg cctcctcaac ttcaatgatt gtgttatact tacattataa
    13021 tagacacagt gtctactatc tataatcaat catctataag tttgtcgatt aagcgacagc
    13081 tttgctttga tatgtcggat aaggagatag gtaaatgaaa aagacagata gacgcgtcaa
    13141 aaaaacagaa aaagctttgg ctgaaacact atccacatta ttagtaaata aaaagattca
    13201 agcaatcact atacgcgaac taacagaaac ggcagatgta catcgttcga ctttttatac
    13261 acattataag gatatctatg atctatatga tcaattagaa agcaactttt ttcgggatct
    13321 aaatgaaatt ctctcttatg atccagccca tagctatgag gaactataca ctcgcttgat
    13381 tgactatgtt tacgcaaata cttttttata tcgcttattt atcctcaacc atggtgatat
    13441 ttcgtttcag aagaaagtta aacaagttat tgaagaaaac tatctgaaca tatggctata
    13501 tgaagacaag aaaacgatta tcacagaaga aatgagattc tttactacgt accacgtcca
    13561 aggttgtcta tccgttctca atcggtgggt agaaagcaat tacaagtact ccaaaaaaga
    13621 attattagaa ctcttacaga aactgaatga tcacatagag aaaatcatgc cttgaataaa
    13681 aacatactaa gcttagttaa gaagaaccgt aaccttctcg tataacaact gaatcttatt
    13741 tagccaaatt gtttactgaa gattttttga gtaaaatttc tataattcct acttattgaa
    13801 aagtagtttg tagtgctgct tctccctctc taagtgatat atcttgttgt accaatcaaa
    13861 ttcacctcat cattaataaa ttacttttag gctaatttat tacaacttat aggttgaagc
    13921 tgtaaccaat ttatagtcat attatatttt tatcatccag tttattgaat tcttctcgtt
    13981 tacctcaatt cattttcaag gggaatcagc tcatcatcta acattttacg ccatgctcgc
    14041 ttcatttctt gtgggtattt aaatggtttc gcttctggat gtttggcttt atatccttcg
    14101 taatccttac tgttgataat tgctttcata ttaggaattg gatcttcacc tggcatatat
    14161 tgcagattag aagcatgtaa ttttttctca gaaacaaacc actcattgga aaagacatga
    14221 atcaaatcat cacttacttg tgtaaaatag cgacgtttaa tgacaaagat atcttcattg
    14281 ctatctagtg taccttcatt tctttgtgct taaattcaaa gataattttc tcaataaaga
    14341 tttttgattc cagtaagatt cctttactcc ttttacaaca tagcgttgtc ctttgttttg
    14401 aaacatcttc ttcccctctt tgccattaaa aatagcccaa ttctctataa agaagattaa
    14461 gcttcattat tataatataa agtttaaatt tggtttatta cttacttttt cttattctct
    14521 agtaagctaa tattgaaaaa aatgtaatca gatcattatt tttatatgtt gtaacttcct
    14581 gataagaaaa ctaattaaaa gaaagaggta actacataaa ctaattaaaa aagctaactt
    14641 catagttgga ggataaatgg aagaaacttc aatgattgtt tctccttgtg ttccttcaat
    14701 cgacactagt cctattatca atctctcttt tttgtccatt tacttttgcg actgttcccc
    14761 aataatcaaa tacaggaagc gttatttttt gtcctttaga ttcatgtact gtatagacta
    14821 ttttcgaatc catcacttga taaggcatag tatgccattc cttgccaatt cttacttcat
    14881 gattctgcaa atgttctttg tattgtaatg cttccgtggg tgcatagtca taaagtcctc
    14941 cgggaaggag tgtcgtgtaa cgaataagat tttcttgatt cattaccaag tgatctctct
    15001 gagtgacaac tttatgttca tagtttgctt ttgttgcagc agatacagcc aacagtaata
    15061 aaacaattgc cgttatatta actacttttc tttgcttagc tgatgtgctc ttcatgaaaa
    15121 cgaacgataa aatcaacgag ccaaacagta cttgaaagcc taagattcgc caaggaaatt
    15181 gaatcatttg aatgggtgta ttttgtaata aagaccatgg aaaaaatttg gtggataaga
    15241 gccaagagac taccgcacta aggtaaatca ctttttcttt gcgattaaat cctttaaaaa
    15301 agaagagtgg acagatcaaa gaaataatac caatgattcc taccgtaaag gttgtcaggt
    15361 cattattcat cgcaccaatc aaactatccg agacatttaa tgcttggtgt tgtaaggtcg
    15421 ttttatctgg ctcattaatc gattgaacca tgatttgtcg gaacatcgga taccaaaagt
    15481 atgctgttaa aagaatcgtc attcctactg cctttgacaa ctccacaatc aaccggatgc
    15541 tttctttttt tcttgtttca cttactacaa agccgcctaa gactagtaag ccaacggcta
    15601 ttacactcat aaaagtggaa agaacatgtg tataaatgag taagctcata ctgactgcta
    15661 atggaattgc acatggcttt ttctcataaa tcagtgcgta agcataatac ataactatcg
    15721 gtataaccgt aagtgcaatc gcttcagcaa tagcagaacg atgataaata tcaatcgtac
    15781 ggtacccata aaatgtatat aaacagctaa atataaaagc cgcatcgatt ctttttaaaa
    15841 attttagtcc ataatgaaac atcagcatga tagtacaaac attcaatagc caaacatata
    15901 agatataact aacgattaaa ttattactta tccaataaaa aatgactgcc ggaaaaaagg
    15961 tcaagtaggg ataaaaataa ttcactccgc tcccataatg attaaacgtc gtaaaattga
    16021 ttggaccaga aaaaattgaa gataaccctt tgactctagc aaaatggaag gcaatatccc
    16081 ccgatgtaga aatagtatga acatttctaa aataattgat tgaataaagt ccagcaataa
    16141 ttgctaggag aatggacaat attatttttt tctttacttg cattacttaa ttatatactc
    16201 ctttcatgga ttgctttatc tagtataact taaattttaa ataaatcgtt atttattttt
    16261 actctcactt aaaaaagcac taaaattata agagagggag gtaatggtct gaacaccaag
    16321 aattgttcca acatcgaatt gcttaactaa tgagttcaaa gcttaattat gtggctctta
    16381 tatttaccga gcgtggattg cgatctcttt agcgaaacaa acatctttca taaaaaaaac
    16441 agccatatcc gctttcttgg atatgactat ttactactac tttactatct ttcattcttc
    16501 ttgtctcact tatgataagg cgctgcgtat catgagtgaa tgatattagg aaaacttcat
    16561 aaaataaatc ttttatacat ttccacttct agatggtcat tcatgttaga agaatcaaac
    16621 tattttttta ctgtcgtaat aatattaaaa cactagaaca aatcaattaa attccataaa
    16681 atataaataa tagacctagc atcatctggt ataaaatgga tgctaggtcc gttatattta
    16741 ctcaaaattg attctacgaa tagatcttct tatcatttct attttagtag tcatgcgttg
    16801 ttgttcttct aaaggtggta atggtataag tagtttagat aaatttgtac catttacatt
    16861 tggttgccca gttccgtaag aaattttttc taataacttc caatataatg gcgagtctaa
    16921 gaagcagtct acgtattctg ataataattt ctcaactaac ctaattcgaa tcaagtaaga
    16981 agcaaaaaca gattcctcac ttatttcctt aaccaagaaa ctttttccca tagttcctcc
    17041 tgtccttgct atcaaaatgt cattttcttc caatcttaaa tcaactagtt tagaattact
    17101 aatatcacag taaggcacac tggaccaatt tacacgtccc tcttgtatat ctgtgattct
    17161 caaaaactta acattgcctt gttttttcgc cgaatctgta tagccatatt gaatcttagt
    17221 agaaatactt cctaaataaa cccatagtcg aacatcgcta gatgattttt cttgtcgttc
    17281 tttatgaaca acagtgatca tcttgagttg tccgactatt ttttagttgc agcaatcttt
    17341 ttgggacgct tatttgcatg aagcaacgtt gctaagtcta gtaataaaac attggctaac
    17401 acaggattgg ctttctcgcg atacgctgcg gcgccacttt caaacaatac tgctagacga
    17461 tcttctacaa caacgatttg ttccaaatac ggtggaaatt tgattcgttt taggatccgg
    17521 ctaggagagt cccgctcgga cactaagagt tccgaggtat aaggaccata agaacgactg
    17581 gctaagagat aatggccata aaaagccaca ccttgaatct tatcagatgt ttttctgctc
    17641 attgtcacgg ttggttggat tttgtctttg cggcgaattg ctgttttcat ttcagctatc
    17701 gtcggcaata gatagaaagt caactgtcct tcacctttta gtgtgaagtt gccagatacc
    17761 agttgatcat tgttttgagt caaatacgat gcttgtggaa tctctgagag atcagttgtc
    17821 agccaataag cgattggctg tcccgtctct tcagatgtat ctgctaagat atcgatcaaa
    17881 cgtaaagcgg ctactcgtcc agtagcagat cctgtgattg tcaaccataa aagttcccgt
    17941 tttgtatcat atactaagcc ccctacatgt ggccgtttag gtaaaatcag cagtttgatc
    18001 ggctgtcctg tttctctatc cagtacatga ataatagaat gatgctgatg gtcatagcag
    18061 taggcagaaa taaacacaaa atcttgagaa aaagccaacc cttgcggcgt catccattga
    18121 caaacctcat tttcgccggt ttccggcacc accattcttg tttctgttaa tccaggaatg
    18181 ttatattgtt ttgcttcagc cttttcccaa gctgcttgaa caataggata ccgttttaac
    18241 tttttttcaa tatcctgatg tatccgttgc tgcttccaat gcttaaactt taggacatag
    18301 taaacaagaa ggccaatgac cagcaacact aaaatcatta ttgctacccg ctgtgtcatt
    18361 tttttgcctc cttgttctat tcgatcaatc gtccatatac cgctattaat taccgtaact
    18421 acatgtttct tttactagcg tttctttata ggatctttca taatctcatt tatagtaaca
    18481 ttaagaatcc cttattctac tatagtcact tccacatcaa tattgcctct tgttgcttta
    18541 gagtatgggc aaaatgcgtg ggcttcttct gcgattttct ctgcttggtc cttctcaagt
    18601 cctggcaaag aaacttctaa aacgaccccg atttggtagc ccccatcttc taattgaaat
    18661 aaagataccc gtgctttgac ttcagaatca gactcaagat tattttcttc cagcatgtgt
    18721 tgaaccgcgc cgttgaagca agatgcgtat ccagccgcaa aaagttgttc cggatttgtc
    18781 ccttcactat ggattcctgg gcggtcgatc ttcatgtgca tacttccgtt tggtgcctca
    18841 acttcgccat cacggcctcc acgattgatc attgctgttt cgtacatttt tttcattgag
    18901 atcatccttt ctttcgtcgt tctatattaa gtgtaacgag tattttttaa tctgacaaag
    18961 gtaatgcttg cattcggcga catcagttag acaacatcga tgatcagtga tagaagcagt
    19021 ttatctatac aaaaaaagaa gggttctatg atatttggtg tccttactcg ccttgagtaa
    19081 caacaccaaa tatcatagaa ccacttaatc agcttcacac tgctttttct actttcgtag
    19141 ttgaaaaaaa cttaaacaaa tcatcggcaa atagttaaag aaaaaactta atcgttgaaa
    19201 gaagcccaat tgcttaaaga aggcaaactg actaatcgac ggaatccgtg ctaatccgta
    19261 aagacctgca attagtaaac taataactgt taaaacaaga taaaaatggc taagcgtacc
    19321 ttgaccactt tgacgataaa gcagaacaag taacaatggg aaaagtagaa agcctgcata
    19381 gcctagccca gaacctatat tatggatcca agtcgataga ttacaagagc tttcattcgt
    19441 attgatactg aataagccag taaaaataca atcacctatc ccatataaac caactgttgc
    19501 aacaacaagt attgcaagtg tttttgatgt tccaacaaat aggtgataca acgcaggcaa
    19561 cgaaagaaca aaaaaaagtc cagatacaac tgaccagacc aagaaagcac gtctgactgg
    19621 actatctacg tcaccaaaca cactaattac ggtagtcatt tgattcagtt tgggataaaa
    19681 aaggccaagg atatacggtg tcgataaatc actcatcaca cctaataata aaaaataaaa
    19741 accatatttt tgcaaaaaaa tcattttctt cccctacttt ttagtgatta ttcgatacat
    19801 ccaacaatca aattttttct cattttaaca catattttta ttctagttgt cttttgaaca
    19861 atcaataaca taaaggtgag tttcttctac attttaaaaa atcgaatggc actgaagagt
    19921 tcagttgtca gcatcaatct cctactcaaa aaggcaaaaa aacgtaagtc agatgttgtt
    19981 gacatctcac ttacgcatcc attgattgaa aatttcttag taaaatctac tagcctaaaa
    20041 atttgaagac ttcttctcga tcatacagtt taaatacttt ctcgcataaa aattgagtaa
    20101 ttagtgccgc aaaaacaggg atgactaacc aacaaagaac aacaagaaag ccatttagcc
    20161 ctgcatctaa agaagccaac ggaccaacaa gtccgaccac gccgaaacca gcagaagcag
    20221 gcgtacctga aatagaaaat agagcgacag gaatagctgt aatcactgca ctaactaaac
    20281 aaggaataag aatcacaggt tttctaaata gattaggcat catcatcttc atagcgccta
    20341 aagcaatcgc aattgtcaca cctgacttat taaccttcca agagttcact actaaaacaa
    20401 aagtggttgc agctacgccc atcgcagccg caccagcaga aacaccatct agttggattg
    20461 ccattccgat tgctactgtt gaaataggag agataatcaa aaaggcaaag gaacaaccaa
    20521 tcaagataga cattaacagc ggttgtaagg tagtgaagtt attaatcaat gatccaattg
    20581 cagaagtaat tgctgttaca aaagggaata aataaaaacc aagtaaacct gcacctacac
    20641 ctacaacaat cggtgttaac acaatcgaca ctgacccaaa actatttcca atcagtaaaa
    20701 tcagcccgac agcaatcgat gctgtcagca tcgtattaat aatatcgcct gtaccagcac
    20761 caacgtaaac acccgtttca cccaaatcag gtgtgaattt gactacacca gaaccaacaa
    20821 aagaagctcc ggctacaacc atcattctca tcggatcaaa tccaaattgt aaggcaatca
    20881 aaccaccaat gatcaaaggt gttgctagtt gaaaaataac tgctaactgt gcgatcgtta
    20941 ccgcaaaggg atacgcacta aaatatttta aaatggcacc taatactgca ttaggaatca
    21001 atccaataat gattcctgtt gctgtaccag cgaggacttt attcagaaaa attttcggtg
    21061 atattttttg tgaagtttcc attcaatctc tcctttattt tacgttcaaa ctatcttctt
    21121 ttgcatggac aagctcagtc aagaaatcac aataaggcgt cgcaacacca tatttcttcc
    21181 cttttctaga aattgcgcca ttgatataat cgatttctgt taatcgatgg ttcttaatca
    21241 agtcttgata cattgaagga taatgtaacc caattgtttc cgggtcaaaa caagattcac
    21301 aatgtgcaat gacttctggg acatctagtt caatcttctc tactgctgct actttggcaa
    21361 actcattgac aatcgtcgcc accattttat gagcagtcga tgtttttcct agctcagcca
    21421 tattaacgtc taagattgtg catagtccgt tcattgttcc gttcacacat gctttgcgat
    21481 aaatagaata gtgaatattg tcagaaaaat gagcatttaa tcctgattca gacaatttgt
    21541 ctgctaattt ttttgcagct gcttcttttc catcacctaa attttgtaat tctactgaac
    21601 cacttccaaa taatttgacc tgaccaggac cttcaagacc tgccgtccac atggtatttc
    21661 caatatagat attttccatt ggtacaaatt tctcaataat atcttcatgc ccgataccat
    21721 ttaatagaca taagacctct gtatcttttt tgattaatga ttggatatcc tgcagcattt
    21781 tttccagctg catcgctttg gtaaatagaa taatcagatc aacttgatct tctttttcta
    21841 cttcggattg aagaacgatt ggtagttttg cttctacctc tttaccatta aaatttgctt
    21901 gtaatccatg ttctttgatt tgttggacat gttctgccca gccatcgatt aataatactt
    21961 cgttcccact ttggtgaagc atcaacccaa agcgacttcc cattgcgcca gcgcctgcaa
    22021 ttgctatttt cattgattct tcctcctttt tctttctatt atactcattt gagaatcttt
    22081 tcacaacctc gttttgttat tcttctctta tttatcatgt ttatatccac cattcaagtt
    22141 attttatcag caaatcaata aaaactggaa tagaatccca atttcatcgt aagagaaact
    22201 agactagcaa taagaaaata gaaacattca tagaaaaaat ctcactcctc tgattataaa
    22261 agagggggca ttaacctcgt gtatactaaa accttccatt cattcgatgg atgattttct
    22321 ttttgtgaaa aaatattcat tgtagtacga tgttgcgcaa cacagacgga tggtattgct
    22381 tattatttat taaaatcgaa caatacactt atttaaaaac ttctttccca gattttcttc
    22441 catttttaca gttataacta gtattatcat gaaaaatagc cttttattgt ctatgggaat
    22501 aataatttaa aattggtact tttttattaa ttagttcatc gcccaataat ctctccagtt
    22561 tcttagcatc tcctgactca accggaatta tccaagccca gtctaaataa ttttttaaaa
    22621 tctcatcata ttttgcaaat atttcattgc catattccgt ccttaaaatt atattttcag
    22681 tgccagacaa gtgatttata ttgagttgat aaaaaagatc tccatctaaa aaaatatcat
    22741 tagaagaaga tttccccaca gtaatgacca ttccatcagc tctatgtgtt acatacaaat
    22801 aattgtatga cctcatttca cctaatgaat caattatttc ttgcccttta gctctaatat
    22861 attgatctct attacctatc tcactcttat ttaacaaaag ttttttcatg tcaaatttaa
    22921 tcggctgttt tatttttgta aattcgcttc tactactaat ccaatccgag attaattttt
    22981 caacatgtcc cagagtaaac tctgatttat tcgctaaata ctttttttgt tgtattaaag
    23041 acatctttcc ccgaagctca aaattgagcc tacatcgatt ctctatctct tcaaaaagca
    23101 tcttatcttc atcattctta tattcatcat tcataaaacc aatccagctc ctcgattaag
    23161 ataatatttt aatctcatac aaaaactagc tctcatcaaa acaaccattg ttttgatttc
    23221 tcatgaaatt tctttatact tttctccgat tataaattcc taaccccttg agaaaattac
    23281 gcatatattt atctccgcaa ggcttaaaat ttcgttggcc ttcttttctc aaaaatgccc
    23341 cgatttcgcc ttttgatgga tagatttctg cctcagcaaa aacgtctaaa atatcatcag
    23401 tggtcataga caaggcgatt tttaattttt ttagcaacac attgttgata tatttgattt
    23461 ccttgctttt ttgcggcgtg tctactatcg gcttatcttt ttgcggacca cgcgccaaga
    23521 gaatcaggcc atttaggaat gcgtcaaaaa cttggttgtt gatggttttt tcatacacat
    23581 tattttctgc attatctttg ttcttatcct gtactttggt cagtaccgcc tgagcttctt
    23641 ctttagtcag tggtacaccg cctagctcaa aagctttaat caaatccgta tctttcaaat
    23701 caatcgcata acgcaatcgc aataacctat cattgttatt catttgttcc tccgaagtat
    23761 ttagtcttta ctttcttacg tactttttgt cttgctaaga tttttaggat caatgataaa
    23821 aaccgcatca cttatgataa ggcgctgggt atcatgagtg aatgatattg ctgaatacaa
    23881 atagtccgac cacattaagt tgatcggact tcataagcat tttattgact aatacttttt
    23941 tgcttaagaa ttgattctaa catcttttgc taaagttaag catcctatag ttcctgcatt
    24001 atctgctaac ttaggtgtga caatgtattc ttcgatattt ggcgtatcga catagccgtt
    24061 aattagttct aaaaaacgtc tttgtacttt ctttttcaga tgttcttgtt gcatgacacc
    24121 accaccaaaa attataatat ctggtgaaag catcagggtc gtattacagg cacattgtgc
    24181 aatataatcc gcctcgattt cccagaacgg atcttctata gaaagatctt gtcccttggt
    24241 tcccgtacgc atctcgatag cgggtccaga tgccaaccct tcgagacaat catgatgaaa
    24301 aggacaagta ccagagaaag tatctcctga atgtctctta actaacgtat gccccatttc
    24361 tggatggcta aacccttcaa caaaaatccc atcttgtata gctcctccac caattccagt
    24421 tccaacagtg taataaacaa cactggataa accttttcca tgacctgaaa catactcccc
    24481 gtaacaagca gcattcacat cggttgtcca ggcaattgga atttcgaaat gcttattcaa
    24541 cgttccaaca aaatcaaaat ttttccaggc agtttttgga gtagaggtga tatgtccata
    24601 ggtctttgat tcacgatgga tatcaatcgg tccaaatgac cctactccaa tgctttctaa
    24661 ttgttcttta tagtgattaa agaaatcaat taccaaactc attgtttcat tcggtgtagt
    24721 tgtaggaaaa ctcactcttt ctaaaacctt taaatcatca ttgccaatgg cacaaacaaa
    24781 tttagtccca cctgcttcta ttgagccata catttcttac tccaccgtcc ttttatctag
    24841 gaattcgttc ttccctgtct aatagttttt cgaaaaactc atgtggttcc ttttggtacc
    24901 aataggcaac agaagaaata tcgtcttgac gctcataaag cttgatgtca tcatttccta
    24961 gggtttggaa agtaacacgt aaattttctt tgaaactaat ggggtcgggt aaatgccagc
    25021 ggtacaaccc atgcatcggt aaagcatcat cgccaaaacc atgaactgga tttggattag
    25081 gttttccagc actaaaataa tcccgggtgt gatctctttt agattgaaaa ggatagccca
    25141 taaatagcgt attaaaattt tgagcatatg ccctttctcc aaagttcttt ttatggaaag
    25201 cccaagcgcc gccaaaataa tcttctgctc ctgtagatgt tattgtaggg aaatcttcat
    25261 ctccatctat ataaaactta aattctcctt ccccccacca gtatctttcc aatgcgcaca
    25321 aagcaaagta ggttcctaca taatacccag tgcctttgat tccatcaata agggtgtaat
    25381 cttttccttt tgtcgtgatg ggttcacggt tccatttagc atgaaaatac atcgcatcat
    25441 ccggtacttc agtcaatgca taattaaatg tataaaaaat gtgcttaata aactcaggat
    25501 gttcattcgt taaagtaatc ttagcttttt ttcgaaatgg catctcaaaa tagcaattca
    25561 tccctgatgt tggattcact acgattggta aggaattcac ttcgcatctt gtcccaaacc
    25621 cattacaaaa aaaatctcct aatggacttt ctacagaagg agtggattca tcatcccaat
    25681 aaatacgcaa aattaaatca cgtaagacga aactcccagc gattgttttg tctgtcactg
    25741 tcatccaaaa atgacgtaaa attccagggc cttccatttc tgctaaagta attgtctctc
    25801 cagcatttat cggaacagaa ggtgaccctt ttctcccaat tcctagatga ctcgctgtca
    25861 ttgccgcttt ccctttttca ccatttttat tttctggtga aatagttcta ctttcctctc
    25921 ccttgtggag ccatatatta tttaaacaat tcattttatt cgtcctctat cctttcttca
    25981 cttttttgat tattcatata gatgatttgt tgttgtgcct ccgcaataat tgctccaata
    26041 tctaaattat catttcctaa ctgcaaagcg attgacattg ccaagggaag attcattcct
    26101 gcaattacgt gcactctgtc agaaatatag gtaaaaaact tttgatttac acttcctcca
    26161 gccatgtcag ttaaaacaat tatttcgtct gtagtatcca ctaactcttt aatttgttca
    26221 tctatatcat caatttcttc gacatatgca cataagctac taatttgatt atgcatacct
    26281 gttaaaaact ctagtgtctc tttaaaaccc tcagctagta ttgaatggct agcaatgatc
    26341 aatcttctca taattcaaat ccttttcctt tacgctaata ttccaaatgc agccataacc
    26401 atcgcaaaaa caataacgag tagaatcaaa atggtcatac tgattttttt cttactgagt
    26461 agataatatg ctgcagcagt aataattact ggaattaatg ttggtaaaat tttgtccaac
    26521 attggttgga ttttcatact aacttcacca aaagtgaatt taagtggcgt tgaaacactg
    26581 ataactgaag caattaatga tccaactact gttagcccca atactgaagc agcttcagtt
    26641 aaataattaa tcttttcacc gtattcagta acaatcttca ttccctgctt ataaccaaat
    26701 tcaaagcctc tcattcggaa aaagaagatt accatgatta aaatgaacca taaaaagatt
    26761 cctacaggat tattttctaa agccatataa gccgcaattg atcccatgat ggttgggatc
    26821 atcgtcatca aaagagagtc cccaatacca gcaattggac ccatcgtacc aactttaaag
    26881 tcattaatcg catcttttgc ttctaatcct tgcttttcct ccattgcaac acaagcacct
    26941 aaaataatgg ctgccagcca aggctgtgta ttgtaatact gaaagtgatt atttaaagct
    27001 tcgatgtatt catcatcatc tttgtataat tttcttaaaa taggtgacaa tgcgtaagtc
    27061 acagatgcac cttgctgtgt ttgataatta aaggtacaaa ctgacatcaa ccaacgatag
    27121 ttcgctttcc taagatcctt tttcgttact tgatattttt cgctcatttt attcatcctc
    27181 cccaataaac ccaacatcag aagctcccgc agaagcagtg atttgtgcat ctttttcagc
    27241 tctatgatac acattaaatg ctaaagcgaa tcctatgatc gccgctccaa taataggtac
    27301 cttcaaaaaa gcggctaaga aaaatccaac aagtaaataa ctaatgtatc gacctgtagg
    27361 cataaagcgt aacaacatcg caataccaac agcaggcaac atttttccgg ctaatgttag
    27421 cccatccata aaccaattag gtaaaacatc caaaacgtaa ttaacagcag actgtcctaa
    27481 agtaacggca ataaatactg gaattgctgc agataaacca aagaaaattg gccccaacca
    27541 caaaacacta ttcattttct taaattctcg tttattcgca tattcctgtg atttgcgaac
    27601 aacaaaacca tttaagattt tgacaattac atctagttgc acccctaaca tacctacagg
    27661 tagtcctatt gctaatccgg cttctactcc aagctggcta ccaaatgccg ttgcaataat
    27721 tgcgcacgtt ccatagtccg gtacagatga gccaccaaaa ccggcaactc ctaatgacat
    27781 caattgaact gtaccgccaa ctatcaaacc tgtttttata tcacccatca aaattcctgt
    27841 gaataatccc caaaaaactg cgtaatttac aaagatcatc ggaatcccat aataatcaac
    27901 atgtttaata aatgctaata tagtcagcat aataatttga aaaatagtaa aatgcattat
    27961 ccttactcct ttatcctaat tttgattgaa tttcctgtgc cttatccgcc ggaacaaatt
    28021 gtgtaacaat ttctacacca gaatccaaca attttttata cgtgctgata ttttcatctg
    28081 atgcataggc tctagaagat agctgtaatg cattatcttt aacaacagta ccgccaacaa
    28141 ttagtcggtc aattctaata ccgttatcta ctaaatgaag gattgtttcc ggctctttca
    28201 cgatgacaaa tactttttcc tgatcgtatt tccccttttt aaaattcgca atcgctgttt
    28261 cttcagtgat aattgataca gccattccag caggtctagc taaacgcata cttgattttt
    28321 ggatgtcgtt atttgcgaca gaatcatcga tcaccatcac tctgtttgct ttagacattg
    28381 gtgcccattg cgtgacaata atcccatgca ataaacggtt atcaactctc gacataacaa
    28441 tactcataaa tacttcctcc tttatctttc attttcttac aagcaattac catgccaaaa
    28501 caaaaagaac ttgataaatc aagctctttt tacataatca taaacgtatt taacctcatg
    28561 aatcgggatg tcgacactat aataatgttc aacgacactg aaagattgct taaaatgtgt
    28621 ctgaaatttt tgaaattcct cactcgtttc attgaaataa atttcatctt ccaaagtatt
    28681 ttgttttact aatcgttcaa ttaagcaact aatgtgaaca tataatccga aacacgtatt
    28741 attatcaaat gaaattccta gatcattttg taagatgtat atagcatctg aaacccgttc
    28801 taataattta gtcggattta atatggtcaa ttgctccatt aaattcgtta gactaaaatt
    28861 cttcagaata ttattgttca aaactttcag ttcattatca ttaaagtgtc tattcaattg
    28921 attggagaat ttacttatat tcattcctaa aatcatatct tcaattgaaa agaattctac
    28981 atcttctaga tgtggattta gtgttccaac aataaaaaga atattgttct ctttacgaag
    29041 ctccgataaa tagttttcat tcactaaaga gtaatagtca cacgtttcta tgacaagatc
    29101 agtatccctt ggaaagctat cttcaagtat cttttttaat ttaaacgaag ttcctaatcc
    29161 gcttgcacaa gaacagacaa tcatatcctt cttaatattt gatgattcat aaatataatc
    29221 cacattttga ttttctttta cttgttcaag aatctgttta agcggtaaat ctaataatat
    29281 ctgatttgca acatcaatag ccattcttgt tgaaacattg tttagtagtc cgaatttttt
    29341 ttgagcgtct agttccaaat ttagataaat tttctccagt gaacccatat caacaagtaa
    29401 aataatttct tcatatccgg tacactcttg taagtatcta ttaattttgt ctgcaatctc
    29461 actacttgaa gtcattagtg gcatatcaat ggcgtcgtat atatatctct caacgatcct
    29521 attagcagta cttgctatac aggttgccgt tagatctcca tgagccaaaa tcagtgctaa
    29581 acgtttttgc tgaaattctt tactacacat taacttaaat aaaataacca aaacattttt
    29641 ttgaattttt gttaaataaa tatcccctat ttgaataaaa cgattgacta attgaacttc
    29701 tcgactacac caactattaa tatcattgat gaaggaatat aatttatcgt ccgatgttaa
    29761 ttggatttga taaatccaag aattcaaaaa gatagacgtt atttgaagat aataatagtc
    29821 aaattctatt tgaaaatctc tctctatttc cttaacatac tgtctaaatc gaatctcttg
    29881 ataggtggga tatgttaaag ctgaattatt ttggatatca acattctttt gtctaaaata
    29941 tctatcatat ttctcaaaaa gctgctgctt atcagtgtct ttcttccacg tttgataaag
    30001 atactctata agttcgttga tattaaaaac ttcgtctact ttttccaaat catccatttg
    30061 aatatatttt aattcattct gtcttttcat aaccttcatg ccgttgaaat tcacccattg
    30121 tggtaaatga tacatctgaa tataaagttc ttccaaatca tctaagaaat aaaaggcatt
    30181 ggcacaggtc aattgaatga tatttttcaa attgccaaca ttttcactaa agcgatagtt
    30241 taataaaaaa tctaataatt gttgagatat cagtattttc tttgctagtc tttcttcttc
    30301 tgatttaaaa aatatcttga ttaactgttt tctttcataa agagaacgac tgtctaatgt
    30361 tggaagtgtt agctttacag gtattcttct caacaaagtt gataatagtt tatcctcagg
    30421 tggttctgat gttgcaaata taatccagca acttgatgtg taccagtttt cattatcacc
    30481 catccgatga aatttcctcg tatccataaa ctgaaaaagt ttctcctgac attcattgct
    30541 caatgcatga atttcatcta aaaataagac tccattatta gctaattctg ctaaaccctt
    30601 tcttccatct tcggctcctg tatatgcacc ttttacatgc ccaaaaaaat tggacaagaa
    30661 aagttctgga ttattggcat attcactaca attaactgaa atgaaagatg ccttctcttc
    30721 tatataatta ttggccaaca aatccttata cacataattt gctaaatagc tttttccagt
    30781 tccagtctcg ccatgaagta aaatgggtaa gccatttggc ggatagtaga ttgctgcttt
    30841 aatttgagca attacatttc gcagacttcc ttgacttccg ataacccttt ctattccaac
    30901 cctaaaagag ctatcatacc atttttccgc ttcctctgaa gagctaaaaa tttgttgatt
    30961 tggaactttc tccggtaaag aaaagaagat tactggacga gtattgattt ttccaaattc
    31021 tccatctttt acgaattcat tcaaatattg agagacaata ttacgactaa catttaattt
    31081 ttcagatatt ttctgtgctg tattgttccc ccatttattt ttaatctcag agttctctat
    31141 gacataattg acaatgcgtt cttttaattc ttccatcaca gcacctcctc gacactattc
    31201 gaattttgcc gacagtattt ttccataaat atgacttgct aaaaaggctt tgtttagatc
    31261 attcaattgg aacgttgctt gttttgcttg atccgctaag acgttgaaaa gatactctgg
    31321 cgaaatcgca taccccaaaa tatcaacgat tgttgcaatc agatcttctt ttgattcttc
    31381 atctgaaagt aattcacgat acaacatcgt ttgtttgctt actgtgtcat attcctctaa
    31441 ggattcatct gctaaaagaa ccacttgttc caacaaacgg tcggataaat atttatagaa
    31501 aatcagcccc aataaataat ttttatattc tgacgcatcc attttactac ggagattatc
    31561 tgccgcacta aataacttct gatttaattc tgcacccaaa aagatacact cctatctctt
    31621 atttaaagtt gctatatacc ctcttatttt agcatactca gttttagaaa aagcaaaaaa
    31681 ataaagaaac ttattcttga taacaagcta cgtgacgtat atccctaaca caaaaatagc
    31741 catatccttt ggtttggata cggctatttg atattactca ttcaattatc aaatcacagt
    31801 aggtgtgaat ctcatcggga tcggcaatat ctacttctga ttaggatcaa aggtgatttt
    31861 ctcaataaag gtgtgataca aagctttaac ttgcttcttc tcactctgga taagaaagca
    31921 atccaaacta gtcaatatct gttggacatc tttgacttga atcggttccc cttgttgttg
    31981 aaagatccct aagatttcat tctctcgaat ttgtaactgt cttcgttttt cagtcaattc
    32041 atttaaacga tcttttaaca tcggaaacaa ttcgggacta tcttccaaag cagcttcctg
    32101 tttttgctct aatagttaga ttttgcgtac cattttcttc ttttattttc cttcgattgc
    32161 tgattttaat atagtctaaa ggaaatttat atttattgta ttctttataa cttcaagaga
    32221 acttcgtttg agtattactg tattttttta attccataag aaaattagtt attacctgcc
    32281 acctaatcag caaatctgaa cctgagtcaa tataattaat attattttcc atctccaaca
    32341 ttgtaagtac tgttggtgtt aatataggga ctttgtacaa ttgtattttt ttgattttat
    32401 catctgcaca aaggagattt tttaaatcta atccttcttt tatttcaaaa gttgaatttt
    32461 ttaaatctat aagagtaaaa ccaattcctt catactgaat ttctgaaaat cgaatttttc
    32521 tcccagttga tagatcaagg ggcagaaaac cttttattac attgtgagtg tcaccatcat
    32581 ttacaatgtc attaggaatt ttgattttag aattctgtgg agcacctatt ggaagataat
    32641 aattcattat aattttgtag tcttcatgag gaaataaagt acttttagga ttgctaagat
    32701 attctcgtac cgaatctaag aatggcttat ccattgaatc ttcatcccca tttaaactaa
    32761 aaaacattcc tacaattgct tttgcaactc taagtgaatt cattttcttt attgaatgtg
    32821 ttaaatcaga cttaccattc tttaaacttg tattgttata aaggaaattt ttattgtctt
    32881 ctgcaaatct aatatattcc tcaacatact cactaccaaa aaaactatta catttactac
    32941 ataacg
//
LOCUS       DACDYI010000026        32618 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.026, whole genome shotgun sequence.
ACCESSION   DACDYI010000026 DACDYI010000000
VERSION     DACDYI010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.026"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            292..1431
                     /locus_tag="IWM52_001192"
     CDS             292..1431
                     /locus_tag="IWM52_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAP9973455.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1498..2073)
                     /locus_tag="IWM52_001193"
     CDS             complement(1498..2073)
                     /locus_tag="IWM52_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9973456.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2078..3052)
                     /locus_tag="IWM52_001194"
     CDS             complement(2078..3052)
                     /locus_tag="IWM52_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAP9973457.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3076..3120)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3335..4303
                     /locus_tag="IWM52_001195"
     CDS             3335..4303
                     /locus_tag="IWM52_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9973458.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4416..4694
                     /locus_tag="IWM52_001196"
     CDS             4416..4694
                     /locus_tag="IWM52_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP9973459.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4812..5048
                     /gene="secG"
                     /locus_tag="IWM52_001197"
     CDS             4812..5048
                     /gene="secG"
                     /locus_tag="IWM52_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAP9973460.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5160..5906
                     /locus_tag="IWM52_001198"
     CDS             5160..5906
                     /locus_tag="IWM52_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9973461.1"
                     /translation="MKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSLAEH
                     QLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5909..8266
                     /gene="rnr"
                     /locus_tag="IWM52_001199"
     CDS             5909..8266
                     /gene="rnr"
                     /locus_tag="IWM52_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAP9973462.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8288..8752
                     /gene="smpB"
                     /locus_tag="IWM52_001200"
     CDS             8288..8752
                     /gene="smpB"
                     /locus_tag="IWM52_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAP9973463.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9054..9773
                     /gene="atpB"
                     /locus_tag="IWM52_001201"
     CDS             9054..9773
                     /gene="atpB"
                     /locus_tag="IWM52_001201"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAP9973464.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9826..10041
                     /gene="atpE"
                     /locus_tag="IWM52_001202"
     CDS             9826..10041
                     /gene="atpE"
                     /locus_tag="IWM52_001202"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAP9973465.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10132..10656
                     /gene="atpF"
                     /locus_tag="IWM52_001203"
     CDS             10132..10656
                     /gene="atpF"
                     /locus_tag="IWM52_001203"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAP9973466.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10643..11185
                     /locus_tag="IWM52_001204"
     CDS             10643..11185
                     /locus_tag="IWM52_001204"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAP9973467.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11208..12764
                     /locus_tag="IWM52_001205"
     CDS             11208..12764
                     /locus_tag="IWM52_001205"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAP9973468.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            12778..13680
                     /locus_tag="IWM52_001206"
     CDS             12778..13680
                     /locus_tag="IWM52_001206"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAP9973469.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13703..15109
                     /gene="atpD"
                     /locus_tag="IWM52_001207"
     CDS             13703..15109
                     /gene="atpD"
                     /locus_tag="IWM52_001207"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAP9973470.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15123..15545
                     /locus_tag="IWM52_001208"
     CDS             15123..15545
                     /locus_tag="IWM52_001208"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAP9973471.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16014..16244
                     /locus_tag="IWM52_001209"
     CDS             16014..16244
                     /locus_tag="IWM52_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAP9973472.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16341..17642
                     /gene="murA"
                     /locus_tag="IWM52_001210"
     CDS             16341..17642
                     /gene="murA"
                     /locus_tag="IWM52_001210"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP9973473.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17655..17831
                     /locus_tag="IWM52_001211"
     CDS             17655..17831
                     /locus_tag="IWM52_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP9973474.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17966..18262
                     /gene="yidD"
                     /locus_tag="IWM52_001212"
     CDS             17966..18262
                     /gene="yidD"
                     /locus_tag="IWM52_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAP9973475.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18326..18793
                     /locus_tag="IWM52_001213"
     CDS             18326..18793
                     /locus_tag="IWM52_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9973476.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            18878..19762
                     /locus_tag="IWM52_001214"
     CDS             18878..19762
                     /locus_tag="IWM52_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9973477.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            19879..20112
                     /locus_tag="IWM52_001215"
     CDS             19879..20112
                     /locus_tag="IWM52_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAP9973478.1"
                     /translation="MSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            20200..20280
                     /locus_tag="IWM52_001216"
     tRNA            20200..20280
                     /locus_tag="IWM52_001216"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20234..20236,aa:Tyr,seq:gta)
     gene            20287..20358
                     /locus_tag="IWM52_001217"
     tRNA            20287..20358
                     /locus_tag="IWM52_001217"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20319..20321,aa:Gln,seq:ttg)
     gene            20873..21580
                     /locus_tag="IWM52_001218"
     CDS             20873..21580
                     /locus_tag="IWM52_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9973479.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(21639..22273)
                     /locus_tag="IWM52_001219"
                     /pseudo
     CDS             complement(21639..22273)
                     /locus_tag="IWM52_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
     gene            22400..22687
                     /locus_tag="IWM52_001220"
     CDS             22400..22687
                     /locus_tag="IWM52_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973480.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            22795..23115
                     /locus_tag="IWM52_001221"
     CDS             22795..23115
                     /locus_tag="IWM52_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9973481.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            23196..24509
                     /locus_tag="IWM52_001222"
     CDS             23196..24509
                     /locus_tag="IWM52_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP9973482.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKVCQEIKEMNEEGKKML"
     gene            24506..25225
                     /locus_tag="IWM52_001223"
     CDS             24506..25225
                     /locus_tag="IWM52_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAP9973483.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYRWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            25353..25904
                     /gene="raiA"
                     /locus_tag="IWM52_001224"
     CDS             25353..25904
                     /gene="raiA"
                     /locus_tag="IWM52_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAP9973484.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            26126..28660
                     /gene="secA"
                     /locus_tag="IWM52_001225"
     CDS             26126..28660
                     /gene="secA"
                     /locus_tag="IWM52_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAP9973485.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            28786..29887
                     /gene="prfB"
                     /locus_tag="IWM52_001226"
     CDS             join(28786..28848,28850..29887)
                     /gene="prfB"
                     /locus_tag="IWM52_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAP9973486.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            29992..30678
                     /gene="ftsE"
                     /locus_tag="IWM52_001227"
     CDS             29992..30678
                     /gene="ftsE"
                     /locus_tag="IWM52_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAP9973487.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            30662..31555
                     /locus_tag="IWM52_001228"
     CDS             30662..31555
                     /locus_tag="IWM52_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9973488.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            31707..>32618
                     /gene="cls"
                     /locus_tag="IWM52_001229"
     CDS             31707..>32618
                     /gene="cls"
                     /locus_tag="IWM52_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP9973489.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAV"
BASE COUNT        10842 a         5687 c         7082 g         9007 t
ORIGIN      
        1 tttttttgca actagcttct gtatatgatg tttacaaaga atggtacaag aaaagatcat
       61 ccttgaaaaa ggaaagctaa caataactag catcatataa gagcttttaa ggatagaaca
      121 gtagaatagg aatctatggg caagtcctct cgtataaata aaataggagg gaagtagcag
      181 catatttttc ataaagtggc tctctttctt catttttact ttggttgctc ctatttgaaa
      241 attcgagcac actcaaatac aataaaaaga aatggcaagg gagaggatta gatgaaaaaa
      301 gaagaaaaaa tcgcgatttt acaagagatt atccgaatta aatcagtgaa tggaaacgaa
      361 ggtgaagtag cagcctattt gaataagctt ttggcaaggc atgacataac aggagagatt
      421 gtttcttaca gagatggtag agataatttg attgctcgtt accaaaaggg ccaatcagga
      481 aaagtgttag gcctatctgg acatatggat gtcgtggcgg ctggtgatga atcgtcttgg
      541 acctatgcac cctttgcagc agaaatccat gggaaccgtt tgtacggacg tggtgctaca
      601 gatatgaaga gtggcttggc agcaatggtg attgccatga tcgaactgaa agagtcagga
      661 aaaccattta acggaacagt caaattatta gccactgtcg gagaagaagt gggtgaacta
      721 ggtggggaac agctcacgaa agcaggctat gtagatgact tagatgcctt gatcatcgga
      781 gagccaacca attacagttt gatgtatacg cacatgggat caatcaacta tacagtcacc
      841 tcgcacggaa aagaagcgca tagctctatg ccagaccaag gttataatgc aatcaatcac
      901 ctaaatgaat tcattacgaa agcaaatgct gaaatgaatc acctcgctga aacaatcgag
      961 aatcctgtcc taggaaaaac aatccataat gttacattga tcagtggcgg gaatcaagtc
     1021 aacagcatcc caagccatgc gcagctccaa gggaacattc gatcgattcc agaataccca
     1081 aatgataaaa tcattgcatt gctgcaatca atcgtcaatg aattgaatca agagacagac
     1141 tatcatttgg aattgatgat cgattacaac aaaatacctg tcaaagctga tccagattct
     1201 cctttgattc attccattca gcaacaattc agccagccat tacctttagt tggagcggca
     1261 gcaacaacgg atgcagccga atttacgaaa gctaaccatt ccttcgactt tgtcgtcttt
     1321 gggcctggtg tcgttacttt gcctcatcaa gtagatgagt atgttgaaat cgataattat
     1381 ttggatatga tcgaaaaata tcaaggaatt attttatcct acttagcgta agattttttt
     1441 aataaatgaa aaaacagagc cagattagca ttattttgct aatccagctc tgtttattca
     1501 gcctattttt ctttttgaat atatcaagcg ttggggcaac atagtgatga ctaatgaaac
     1561 aacgacgata gacaacaagc gatcaaggta gtctgtaccc acctgtacca agatcacact
     1621 tgttgtctga cttaaaccta acccatgtaa tagctgaacg atcatactgg atcctgagga
     1681 ggtgatcccg ccaaataact gaacggtaat gatcgaagaa acgatcgttc caggtaccga
     1741 caaaatcaaa gcgagtaata acagtttcaa gctgttttta tctttgccga gatatcttgg
     1801 caaaatcaat ccagcaagca agcctgtgag aatttggatt ggcgaataat acagagaaaa
     1861 aatatctgta gtcactccac ttaaaatacc gctggacaat cctgtcagca ttccaggtac
     1921 aggcccaagc aatgcaccag caaaaatcgt tccgatagaa tcgagataga ttggcaaacg
     1981 aagccataaa gcaatcgttc ctccaatata attgatccca atacacaaag ccatcactgt
     2041 aagtacgcga gttgccattt tgtttttatt cattttgtta gcccatcttt aacctttcca
     2101 aatctttcat aatcgtttct tcttgtgcat ttaaaagaac ggttaaaaac tttcggaaaa
     2161 aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc caaaattcat
     2221 aacgatcaac caatgtttgt ccgcgactta ttccttgagt ttctacagca gtgaagcttt
     2281 taaagccttc acagatggac tcatcgataa aataagcaac agctaatgga tcattgatga
     2341 cacatcctaa gattctttct tgtttccagt gaaagtcaaa ataaaaacga gtgatcgctt
     2401 ttagatactc tccctcttca ggattcattt ggcagcaata ttcaaggata gtcggtgtca
     2461 agacgatttc tcttgtcaca tctaacccaa ccatttctat cggttgtttg agcttttcga
     2521 atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact ggtgaacagt
     2581 taccatggct tttgtaagtt ccgcccattg agacaaaacg atccatatgt ttcccaacag
     2641 atgggctgtt tttcaaagca gaagcgatat tggtcaaagg ccccaaagca atgactgatg
     2701 tatctgtttt ttctttgaaa taatcagtca gaaaatcaac tgcatggagc gattctgcct
     2761 gtttggctaa gaccatcggg aaatttgtgt cccctaatcc atccaaccca tgagtgtctt
     2821 gcgcactaat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca gggatatcta
     2881 aacgatcaca gcgctccaaa catttcagca cattctgcgt accgatatct acagggacat
     2941 tcccgcaaac gacggtaata gcaactactt cgatatcagg ggatttgaca gctagtgata
     3001 aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tactttacgc attatttcca
     3061 atctcctttc tttagagata cttagttttt tattctggga agtttccgaa ccagtccaag
     3121 acgagtgtct tgtttataat gcacgaaatt agtatactgg cttttattgt gaatgtaaat
     3181 gctctcttac aagtaaatct attgagaaga actttaagta aatgatacaa tgaaaaagaa
     3241 atggaattta cgtgtgccga agaaaaagaa tcctattgat cggtatattg tataataaat
     3301 agattatagt ttggcagaaa gcggcgggtg tacgatggtt attttttatt tcattcttat
     3361 tctgatattt ataggaatca gttaccattt tctttcttat ttttatgcaa agaaattagt
     3421 aagagcgggg gtacagagag gctatgactg gtatgaagaa actgggcacc gcttgatgga
     3481 tcctgctgca gtcacgtcag tagaaaaaaa gcgacaaaaa ttagaagaag agtgtgaaag
     3541 cttctgggaa caaggaatac cgaaaacgat caaaagttat gacgggttga aactagctgg
     3601 acagatgttc ctccaaccaa ctcagcagaa taaatgggtc atttgtgtac atgattatcg
     3661 aagtacaggg aaaagagata tgtctcatat aggtaaaaga tatgcagaga aaggattcaa
     3721 cgttcttata cccgatttac gtgcgcatgg agaaagtgaa ggagaaatca tcggaatggg
     3781 atggctagac cggctagatt taattgcatg gatccaactc atcttagatg aacaaccaga
     3841 tgcatccatt attttgcacg gcagttcgat gggcgcttct actattatga tggcaagtgg
     3901 tgaaaagctt ccatctgcag tcaaaggatt tatcttagac agcggatatg tttctgttta
     3961 tgcggaattt cggtatatgt taagtaaaat cacggtcttt cctaaaaaaa tgatcatgag
     4021 atatgcgaat cactatgctc agaaatacgc aggttattct ttaaagcaag catcagctac
     4081 aagacagctt ggtagtaacc atttgccttt gttagtcatc catggtgaaa gagatcattt
     4141 tgttcctaca gaagcagcat acacgattca aaacgctacg gcaggggata aagcattact
     4201 gcttgttcca gaagcagaac atttggaagc atcgatgaaa gatccgaaaa cctattggac
     4261 agtgattttt tcatttattg aacaaagaat aacgatggat tgattttctc ttcctttttg
     4321 taagcaacac gaggataaat aattgtttat agaccatgag actgttaaaa taaggtagga
     4381 ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagag gaaaaaaatc
     4441 gtcttgcttt attcaatgat gagcaacaag aaatcggaga aatgacatgg tcggatgcgg
     4501 ggccagacat catgatcatt gaccatacat ttgtcgatcc agcatatcgt ggtcaaaaat
     4561 tagctgaaaa gctagtgtat actggagtag aactagcaag acgagagggg aaaaagatta
     4621 tcccttcatg cccttacgca aaaaaagagt ttgagaagaa accagagtat catgacgtct
     4681 tacgccaatc ataataataa cagctgaaag atttgcactt tcagctgttt ttccatttat
     4741 atagagttgt cacggagttt tatctatgat aaagttaaag agacgatatc ttttgggaag
     4801 gaggctgcta tatgtataat ttaattttgg gaatcgtgat cgttttatcg attgttatgg
     4861 tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca tttaccggcg
     4921 gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc atgcagcgat
     4981 caactgcagt catgggagct gtctggatga tcttattatt tgtcctagca tttttatctt
     5041 cgaagtagtc gaataagcct ctcgcacaac ggcgggcggc tttttttatt gcaaagagaa
     5101 cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag gtgaaacgag
     5161 tgaaacaatt accaaaaccc ctttatgcaa aacatggaaa acgagcagtt ttgctgcttc
     5221 atgcgtactc aggaagtcca aatgatgtcc ggatgctcgc acggttttta gaaaaagcag
     5281 attatacagt ctatgcgccg ctatttaaag gacatggaac gatggagcca tatgatatct
     5341 tgcaagaaaa agcagaaagc tggtgggcag atactaaaaa agctatccat tttttacagt
     5401 ctgaacagtt ttctgatatt gccgtattgg gattatcgat gggtggcatc tttgctgtac
     5461 gggcactaga agaagaatca gtaattggcg gcggtttttt ctgttcgccc ctgtctcctg
     5521 tagaaacaaa cgtgcctgaa aattttgaaa aatatgtacg tcaagtgctg aagatagcag
     5581 gcaaatcaga aaaagaaatc aatgaaaaag ctgtgtctta ccgctcgtta gctgaacatc
     5641 aattaatgga tatacaagac caagcagcaa tcgcagaaag tagactatca gatatccaac
     5701 agccaatatt tttagctcaa gctgggaaag atgaaatgat cgacccaaat ggtgtatttg
     5761 aaacggcacg caaattgagc cgccaacgag tgacacttca atggtatcct gaaagcggtc
     5821 atgtgattac agttggcaca gcaagacgcg aattagaaaa agacgtgttg gaatttttag
     5881 aaaaattacc ttggaatgag gaataaatat gacaaaagaa acaataaaag agaaaattct
     5941 ctttttcatg gaaagcagtc gcaaacgtag tttttctatg gaagaaatcg ctgaaggtct
     6001 aggctttcaa aaaagcgatg attttaaatt actagtacaa acgattgctc aaatggaaag
     6061 agaacagagt atcgttttca ataaaaaagg aaaagtcaaa cttcctttaa aaccgatctt
     6121 ggtcgaaggg acatttcggg caaatgaacg aggatttgga tttgtaacga ttgatcccga
     6181 agaagatgat atctatatcc cgaaagaagc gactggcttt gcaatggatg gggataccgt
     6241 agcaattgat atcatgaagc gagcaaatac agatatggat cgcggagcag agggaaaagt
     6301 agtggagatt cgagaccgag cgactactca gttagccgga gaatttgttg cttattcaga
     6361 cgaagagata caggaaacgg atctttatgg tgtggtcatc ccgaaagata gaaagcttaa
     6421 ccaattcaaa gtgtatgcag cggcagaggg agttcgtcca gttgacggaa gtattgtatt
     6481 gatcgaactg acccattacc ctgaaaaagg gtatgcaact agtttagaag ggataatcaa
     6541 gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcgattg tcgtggctaa
     6601 cggtattcct acaaaatttc ctgatgatgt gttatctgag gcagatgaag ttccagacca
     6661 gatcagcgaa aaggatattg ttggacgcag agatttaaga gatcagctca tcgttacgat
     6721 tgatggagaa gatgcaaaag atctagatga tgcagtgacg gtcaaaaaat tagaaaatgg
     6781 taattatttt cttggagtac acattgcaga tgtatcttac tatgtgacgc aaggcagtca
     6841 actggacaga gaagcatacg aaagaggaac aagtgtctat ttgaccgatc gagtgattcc
     6901 gatgatccca caacgtttat ccaatggtat ctgttcattg aatccccatg ttccacgact
     6961 caccatgagt tgtgaaatgg agatcgatcc aaatggacaa gttgtctctc atgagatttt
     7021 tccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc aaatcttaga
     7081 agatcaagat gaagaaacga tgcaacgtta tgctcagctc gtgcctatgt tccaagaaat
     7141 gggtgaattg catcgaatcc ttgaaatgat gcgaatcaaa cgtggagcga tttcttttga
     7201 ggatcgtgaa gcgaagatcc ttgttgatgc tgaaggacac ccaaatgata tccagctaag
     7261 aagcagaggg ataggtgaac gcttgatcga atcctttatg cttgctgcca acgaaacggt
     7321 agctgaacat tataataaat taaatcttcc gtttatttat cgaatccatg aacagccaaa
     7381 agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc tggtccgagg
     7441 aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag tagaagacaa
     7501 accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag cacgctattc
     7561 agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact ttacatctcc
     7621 tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca gtgaagataa
     7681 aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag atcatagttc
     7741 taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga agaaagcaga
     7801 attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg ttgtgaaatt
     7861 cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca atgaattgaa
     7921 acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa gaactggtct
     7981 gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc cagatgaacg
     8041 agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac gtccaaaagc
     8101 acgcaatgga aaatctggaa aacgagaagg aaaacaagca ggcaaacgct cagaaaaaaa
     8161 tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga aaaaagggaa
     8221 aaagccattt tacaaaggtg tcaaaaagaa gaaaaagaaa aaataaaatg agttggagga
     8281 aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag ctcgccatga
     8341 ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg agatcaagtc
     8401 gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca acggggaagc
     8461 tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta atcatgatcc
     8521 gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg aaaacgaatt
     8581 gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg gctatgccaa
     8641 ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag atttgaaacg
     8701 aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat aatttcatta
     8761 tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg ttagcattta
     8821 atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg tgcatgggga
     8881 aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga aaatgttttg
     8941 aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg aaaaaaccag
     9001 atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat ggattggatg
     9061 aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt tgtataatgg
     9121 tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga aatcttaagt
     9181 tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt cgaggaatcg
     9241 taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca tttacattgt
     9301 ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc ttacctaacg
     9361 aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact ttggcattta
     9421 taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa ggatatttcg
     9481 taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt gaagaattta
     9541 caaacttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg gaagttttgc
     9601 taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca ttagcaattc
     9661 cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa gcatttgtct
     9721 ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa taatactcgg
     9781 ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa ttatatcgca
     9841 gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg tcaagttatc
     9901 tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt ccgtacaaca
     9961 atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt tatcgccttg
    10021 attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat cgtttgttac
    10081 tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc tatgctgaat
    10141 caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt tgtcagcggc
    10201 tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat cagcgatatt
    10261 ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca atctcgcatc
    10321 aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg ttctgatgca
    10381 gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa tattttgaag
    10441 gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat cactgtagaa
    10501 cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct tcaaatcgcg
    10561 gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat caatcaatac
    10621 attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa gacgttacgg
    10681 gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct atcaagatct
    10741 cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt taagtgatgt
    10801 tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg gttatgatgg
    10861 aatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg accttttgtt
    10921 catgattgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat tgggaagtgt
    10981 cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga atgttgcaaa
    11041 aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta ttattggtgg
    11101 cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc aattagaaaa
    11161 aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg gccatcaaag
    11221 cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac gtgctttcag
    11281 tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca catggtttag
    11341 aaaacgcgat gagtggtgag ttgcttgaat tttcaaatgg ctcatacgga atggcgcaaa
    11401 acttagaatc aaatgatgta ggtatcatta tccttggcga tttcgagtcc attcgtgaag
    11461 gagataaagt aaaacgaaca ggaaagatca tggaagttcc agttggagag gccttgatcg
    11521 gacgggtagt taatccgcta ggtcagccaa tcgacggact aggtgaaatc gttactgaca
    11581 aagctcgtcc agtagaagca atggcaccag gtgttatgca acgtaaatct gttaacgaac
    11641 caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc ggacaacgtg
    11701 aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgatcgat acgatcatca
    11761 accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa gattctacag
    11821 ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca atcgttgtga
    11881 atgccggtgc gtctcaacca gcgccgttgc tttatatcgc accatatgct ggtactgcaa
    11941 tgggtgaaga gttcatgtac aacggtaaac atgtcttgat catttttgat gatctttcaa
    12001 aacaagctgt tgcttatcgt gaactttcct tgctacttcg ccgtccacca ggtcgtgaag
    12061 cttatccagg ggatgttttc tacttacact cacgcttatt ggaacgtgca gcaaaattaa
    12121 gtgatgattt aggtggcggt tcaatgactg ccttaccatt tgttgaaaca caagctggag
    12181 atatctctgc ttatatccca acaaacgtta tttcgatcac tgatggacaa atcttcttag
    12241 aaagcgactt gttctacgca ggtactcgtc cagcggttga tgccggacta tctgtatctc
    12301 gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact ttacgcctag
    12361 acttagcaag ttaccgtgaa ttagaagcat ttacacaatt cggttctgac ttggatgcag
    12421 caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa caaaaattgc
    12481 atgcaccgct agctgttgaa aaacaagtag tgattttgta tgctttgaca catggattct
    12541 tagatagtat ccctgtagac agtattttag acttcgaaca tgaattattc gaatatctgg
    12601 ataccaatca ttcagatatc tttgaaacaa tccgtacaac aaaagatctt ccagaagaag
    12661 aagcgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca aaaggaagta
    12721 attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt gagatgaatg
    12781 ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac gagccaaatc
    12841 acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc ttcttcacag
    12901 aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac tgctactcag
    12961 ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag catgctgctc
    13021 agccgtcctg ttaagaaaac tggttatatc gtcatcactg cagatggcgg tttagtcggt
    13081 ggatataaca gctcgatatt gaaacaaacg atgtctattt tagaagaaga ccaccaatca
    13141 cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt taaagcacgc
    13201 ggtatcaatc tagcatatga gctgcgcaat ctatctgatc agccaagctt tgatgaagtc
    13261 cgcaaaatcg taaatatggc aacaactatg ttccaaaacg aagtattcga tgaattatac
    13321 gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga aaaaatgctg
    13381 ccaatctcag acttagatcc agaagaagca actactttcg aacaagaata tatttttgaa
    13441 ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag tttgatctac
    13501 ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc aatgaaaaca
    13561 gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttataa ccgtgcaaga
    13621 caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc actagaatag
    13681 tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa tcggtcccgt
    13741 tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg cattagttgt
    13801 ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag aattagggga
    13861 cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag gaatggaagt
    13921 cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag gtcgcgtatt
    13981 caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg cggaaagaag
    14041 cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag aaattttgga
    14101 aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga aagtcggact
    14161 attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc ataatatcgc
    14221 ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc gtgaaggtaa
    14281 tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca tggtgttcgg
    14341 acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt tgacgattgc
    14401 ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca acattttccg
    14461 tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt ctgcggttgg
    14521 ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca catctacgaa
    14581 aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact acactgaccc
    14641 ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac gtaaattaac
    14701 agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg ctttagcacc
    14761 tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg tattgcaacg
    14821 atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat ccgatcaaga
    14881 aaaagtgtta gtttctcgtg ctcgcagagt ccaattcttc ttgtcacaaa actttaacgt
    14941 ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa cagtgaaagg
    15001 attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt tccgaagtgt
    15061 tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga aaggggtgcc
    15121 ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc tatgatcacc
    15181 atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg cctaaacatg
    15241 caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca gattcagata
    15301 cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat aatgttgttt
    15361 caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt gcggaacgtg
    15421 caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat acgaacgaat
    15481 tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta tctaagcact
    15541 cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag gttatgacag
    15601 aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca aatttttgtg
    15661 aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatca gaggttgtga
    15721 aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct catattttgc
    15781 cgttttttga gctaataccg agggtcagat gattgaacac cgtttatttg gaaaaaggga
    15841 ctgcgacaag acttttgtct cagtcccttt ttttgcatat ttttagcatt tatttcctaa
    15901 aaaatgtaag gggattccta tatttttaag tatacttgtt tttcctttta tacattttat
    15961 ggtattatca ttttattagt tattataaaa caatcgttag aaaggacgtc attatgcaag
    16021 tatacggaat tgatgcggtg cttcgaattg tgagtcattt ggcgtttatc tacttagcct
    16081 tctggtcttt acgttcgtta cgcattgaaa cttttttcag accaatgcat aatacgcaaa
    16141 ttagaatggc aatcgtactt ttttcaattt ttttagggta cactgcaagt aattttttct
    16201 tagaagttat cgctttatgc cgaaatcttt tcgttagcct gtaaatttta aaaaaaggta
    16261 ctattttaaa agcaaggatg ttataataga aaagattttg agtaagtgta tgctcattgg
    16321 aattttttgg agggaacaac atggaagaga tcatcgtaag aggtggcaat caattaaatg
    16381 gaacagtaag aattgaagga gctaaaaatg ctgttttgcc tattttagct gctagtttgt
    16441 tagcagaaga aggtattacg acattagaca acgttccgat tctttctgat gtcttcacta
    16501 tgaatcaagt gattcgtcac ttaaacgtag atgtagattt tgatgaacaa aagaatcaag
    16561 taacaatcga cgcttcaaga caattagaaa tcgaagcgcc ttatgagtat gttagtcaaa
    16621 tgcgtgcttc aatcgttgtg atgggtccgc tacttgccag aaatggacat gcaaaagtag
    16681 ctatgcctgg tggatgtgcg attggtaaac gaccaatcga tttgcattta aaaggatttc
    16741 aagctttagg agcaaaaatt attcaaaaaa acggctatat cgaagcaatt gcggatgaat
    16801 tgatcggtaa cacgatttat ttagatttcc ctagtgtagg tgctactcaa aatatcatga
    16861 tggctgccgt aaaagcaaaa ggaacaacca tcattgaaaa cgtagctcgc gaaccagaaa
    16921 tcgttgattt agcaaacatt ttgaataaaa tgggcgctca agtctatgga gctggtactg
    16981 aaacaatgcg tattgaaggc gtggatcatt tacatgcagt caaccactca atcgtacaag
    17041 atcgtatcga agcaggtacc tttatggttg ctgctgcaat gacacaaggc aatgtgctga
    17101 ttgcagatgc tatctctgaa cataaccgcc cattgatttc taaattgatc gaaatgggtg
    17161 cagaaatcat tgaagaagaa ggcggagtcc gtgtcatcgg accaaaacat atcttaccta
    17221 cagatgtaaa aacgatgcct catcctggtt tccctacaga tatgcaagct caaatgacag
    17281 ctatccaatt agttgcagaa ggtacaagtg tggtgactga aacagtgttt gaaaatcgtt
    17341 tccaacattt agaagagatg cgccgtatga acgctcatgt caaaatcgac ggaaatgtag
    17401 caatcatgga tggaaaccat gaattgcaag gtgcagaagt atatgcaaca gacttgcggg
    17461 cagcagcagc attagtttta gctggcttga aagcaaatgg aatcacacgt gtgcgtaact
    17521 tgaattatct agaccgtgga tattataatt tccatatcaa actacagcaa ttaggtgcag
    17581 acgtcgaacg tgtagacatg gatcaaactt ctgctgaaaa aacagcacaa acgattgctt
    17641 aaggaagtgg ctgaatgagt aaccaacgat atatcttatt aactttgata aagattttag
    17701 tggtgatctt attgcttatc cttctttttg tggccggaac gatgatcgga tatggtgtca
    17761 tcggtggcgg caatccattt aaagtattcc agccaagttt gtggattcat attcgagact
    17821 tttttcattg ataaataaga atcacagtgg ttctagtcga tatgaagtga gccatgaagc
    17881 agaacatgat aaaagaaaac agaagggaac gttgacgtta gtcgcgatcc ctttttttag
    17941 taaaagaaaa gaaggaatct aattgatgaa gaagatattg atttatttag ttagaggata
    18001 tcagcgcttt atttcgcctg catttccacc tagctgccgt tattacccaa cctgttccaa
    18061 ttatatgata aaagcaattc aaacccatgg tgcgctaaaa gggacggtaa tggggactgc
    18121 acggatctta cgctgtcatc cttttgtcaa aggcggtatc gattatgtac cattgacatt
    18181 ccgattaacg cgaaataaga aagataaaaa agaaccagat tataaaaagg ttgtgaaaaa
    18241 tacgggtagc actgagcgat aaagaaaaag aggaaattta ttgtcaaagt aacaactccg
    18301 caactactta tatgggggac aaaaaatgat cgaagaacta aacaagaaag atttaccatg
    18361 gtccttgtta ttagatgcag atcctgataa agaaaaagtt caagtatatg tttccagagg
    18421 gagtggctgg atatggaaag aaaaagaaaa aacaataggt gtactgatct atgtggcaag
    18481 ggaaaaagaa tacgaaatcg tcaatgtagc agtaacccca acgtatcaag gaaaggggat
    18541 tggcggaaaa ttacttgaga cagcttttca gaagctttca cagctagctt caaaccaaac
    18601 tcggatcatt atacgtactg gcactactag tagcgctgcg ttacacctct atcaaaaaat
    18661 gggctatgtg gagattggtc gagtcaaaga ctattttatc catcattact ctgagcccat
    18721 ttttgaaaat ggagatcaat taagggatca agtgatttta gcaattcact tacctaaaaa
    18781 tagctttaac taataaactt tcaattcgag aaaatgcttt caaatgctaa atagatacat
    18841 tacaatagct ttagcaaaca aaaaggagaa tatttttatg ggaatattag tttttgattt
    18901 tggtggatcg gctgtcaaat atggttgttg ggatggcaaa gagattaaag gtaagggaca
    18961 atttgcaacg cctgaatctt gggaagaaat gaaaacgcaa ttatttcaag tttataaaaa
    19021 aatactcgtt tcttttgaag tcgaaggaat tggaatcagc tctcctggag tagtgaataa
    19081 tgagaaacag gtgattgagg gaatcagtgc gattccttat attcatggat ttaatatttt
    19141 ccgagatttg gaaactctgt ttcaactccc agtaacgatt gaaaatgacg ccaactgtgc
    19201 cggaatggct gaattttatg aaggtgctgc ccgtgattat caagatgttg cttttgttgt
    19261 ggcaggaact gggattggcg gggcgttgtt cacgaaagga caaataaata aaggggcaca
    19321 tctttacggt ggagaatttg ggttgatgtt cttagatggc gataagacat tcagtaaact
    19381 tggtacagcg gtacagatgg cttggcgtta ttgtgaacgc aaaggtctcg ataaaaatgc
    19441 ttataccgga aaagatgtct ttgaattagc agaaactgga gatgcaatag caaaagaaga
    19501 agttgagagt ttttatacgt atcttacaaa gggattattc agtattcaat tttcgtttga
    19561 tccagaagtg atcgtattag gcggcggggt ttctgcaaaa gaagggctca ttgatgagat
    19621 caaaagccga atgaagaagc tgacagaaca attcgatctg catgatttcg aaccgcagat
    19681 tttattatgt gagtacagaa acgatgctaa tcttgtaggt gcagcagcaa actatctcgc
    19741 tgtacgtcaa ggagagaatt aagagcttta gagaaggtgc agcttgcata aaagaagaga
    19801 atgaaatgaa taggagattt ttacctaagg aatgttttat ttgctgcagt atgataaaat
    19861 atctgagagg tgaaaatgat gtcaaaaaaa aataaagata ttgaagtgcg tatagaagaa
    19921 gtgaaaaaag atatcaaagg taagaattat gaagtcactc agttatttat agggaaaaaa
    19981 atgattggtg agatcttagc ctatgggcca aaagaatatg aaattttctt tggtgaagaa
    20041 gattttggaa aagaaaaaag tcttgaaaat gcgatagaaa cagtgattcg acactggaat
    20101 ctgcacgaat aaaaaaatat caaaaaaact ttcaataaag ggttgcaaaa atctggaaaa
    20161 ttaggtatta taactaagtc aggttgatac aaacctttcg gaggagtagc gaagtggcta
    20221 aacgcgacgg actgtaaatc cgttccttag ggttcagtgg ttcgaatcca ctctcctcca
    20281 tttcattggg gtatagccaa gcggtaaggc aagggacttt gactccctca tgcgttggtt
    20341 cgaatccagc taccccagta taccacaatc gtaagctctc gttagttcga gagctctttt
    20401 ttttgctttg ctaccttgtt ccttgaagat tctgctgaat taagatagag ttaagttaac
    20461 tacaagaatt ggagggatcc acatgaaatg gaaagaaaaa gtagacgaag cagctgaaaa
    20521 gttatatgat ttggttaaat cagaaaaata caatattgaa gtgaatattc cgaaaaaagg
    20581 cggaaaagcg gtccaggtaa aatcaaaacg cccaacgaat catacaaaaa aatggatggc
    20641 taaaaaccgc taataataac aaaagatcca taagtcggca ttttcatcga ttttagggtc
    20701 ttttgttatt ttctaggttg aacatatcta aaaaattgtt tctgacaata ggagtaggga
    20761 gaacgatgtg tgtctaaatc ctctgttgat ggatagaaac ctttatcttt ttcagttgtg
    20821 aatcaggtat ataccagtta taatagaaga aacgaaagga cgggtgtacg ttatgtcgaa
    20881 tcaattacct gtatatatac agattcatga ccaattaaaa aatgaaatcg aacaaggtat
    20941 atggaaagtc ggagaccgtc ttccttcaga aagagaatta gcagtgaaat tcaatgtcag
    21001 ccgtatgacg ttgagacaag caattcaaaa cttggcagat gaaggaatct tagaaagaaa
    21061 gatcggttcg ggtacgtatg ttgcgcgaga aaaagtacaa gaaaaaatgt ctggtgcaac
    21121 aagttttaca gaaattatgg aatcacaaaa tcgagtaccg tctagtcgga caatctctta
    21181 tttcttgact tcgccaagct caagtgaaat ggaaaaacta aacttgagta aagatgacac
    21241 gatcttgcga atggaacgta ttcgtttcgc agatgatgtc cctatttgtt ttgaagttgc
    21301 cagtgttcca caaaagctga tcgatggcta cagcaaagca gagattactg aatcgttgta
    21361 tcgcacgcta gaagaaaaag gcgggaaaaa gatcggtcca gcgaatcaaa cgattactgc
    21421 tgtgttggct tctgagaaaa ttgctgacta tttagaggta aaacgaggag atgcagttct
    21481 tcgtttgcgc cagatttctt attttgaaga cggtacgccg tttgagtatg tgcgtacgca
    21541 atacgtagga aatcgttttg agttttattt ggaaaaataa aaaacaggtg atccctgatt
    21601 ccttattaga aataaaagag aatcagaatc acctgttttt atatatgatc agttatcagt
    21661 tgctcgtgat aagaaacaga accctcttca aattggactt gtccgttcag cagctcaatg
    21721 atggattctt taaagtggtc agcacttttt tcatctacag cacagatgac gctaacttga
    21781 tcagtatatt ggatttcttt tataaaaaaa tgttggagtt caagataatt ctgtaatttt
    21841 cctagttgcg gatacgtgag atctcctgtt gcaaggtacc ttctacgatt ccaatgtcag
    21901 acaatgcttg agagaccgca tgagtgtatg ctcgtattaa tccgcctgtg ccgagtttcg
    21961 ttcctccaaa atatcgagtg actactgcag ctacattgat cagttgattt tttttgagga
    22021 cttccaacat cggtacgccc gccgttccac ttggttctcc atcatcgctg cttcgctgga
    22081 tttcattttt ttctccaatg atgaaggcgc tgcagttatg gttcgctttc caatgttctt
    22141 tttttatttc ttgaatgaaa ctttttgctt ggtcctctga ttctattcgt ctaagagaac
    22201 agataaagcg tgattttttg atctcaatct cactttgtcc attttctttt attgtacgat
    22261 agcgctcgat catcttgtca cctctttttt tcattttctt tattcagtat aaataaagtt
    22321 ctaggttgtt tcaacactgt aacggttgcg caattgctgt agtatgcgat aataaagaca
    22381 aaggcaggtg gatctgggga tgcaggaacg aatgaaaaag tacgatgcaa ttacgcatta
    22441 tttgaagaac aatggcggct ctcaagtaac gttgaccttt acccaatttg acgaactgct
    22501 gtttcccagt aacgggcttc ccaaaacagc aagggaaagt acagattggt gggcaaatga
    22561 ttataaacac cctgaaaaag gtgcctatgg ctggataaat gcaggctatg aagtagttgt
    22621 catcaatttg gacaaagaat atgtcgtgtt caataaatta gtgaaatcta gttggctatt
    22681 tgattaaata aaccgcgttt agaaaacgtt gacaaatttc tttcttttta tataatagag
    22741 taacagacgc gaagaaaacg tttgcatagt ttgttttaaa aaggagagaa aagtatggct
    22801 aaaaaaacaa ttatgcttgt atgttcggct ggtatgagca caagtttact agtaacaaaa
    22861 atgcaaaaag ctgctgaagc aaaaggaatc gactcggata ttttcgctgt gtccgcttct
    22921 gacgcagata acaatcttgc aaataaagac gtggatgtgt tgttattagg cccacaagtt
    22981 cgctttatga aagctgattt tgagaaacgt ctggaaccaa aaggcattcc tttagatgtc
    23041 atcaatatgg ctgattatgg tatgatgaat ggcgaaaaag tgttagatca agcaatcacg
    23101 ctcataggtg aataaataga acagtataac tatcgaacaa gcgatccaaa aaatagatcg
    23161 cttgtttttt cgtatatttt tttagggcga agaatatgaa tcatttttgg ggtagaaggc
    23221 ttctgacgag agatattgaa gcaatagact gtgaggaggt aaatttacca aattatgaga
    23281 agatcgcagc agtggaacgt ttgggcaact ggattctttg tcatcgctgt ggaatgagga
    23341 cgtccgtcat tgatggacag cttgcagatt acggttattt ttgtacacat tgtctatatt
    23401 tagggcgatg cgacactcaa caagatttat atttgtttga tcagcctgaa gcaaaacctc
    23461 gagaagtcgt ctttttttgg acaggacagc ttactgaaaa acaaacggaa atctcaaaga
    23521 gaatactttg ccattcagaa aaaagacatc atcttatttg ggctgtaaca ggtgcaggga
    23581 aaacagagat gctttatcct attttagtgg aaactttaaa agctggagga agagtagcga
    23641 tttgtacacc gcgagtcgat gtttgtaatg aattgttttt acgctatcgt tatgtttttc
    23701 cagaagaaaa aatcacgttg ctccatggga atacagcaac ggcttatacc tttacttctt
    23761 ttgttatctg tacgattcat cagctatacc gtttttatcg gtcctttgat ttaatagtga
    23821 tcgatgagat cgatgcgttt ccttataacg gggatgcagg attagctcat gctgtgcaaa
    23881 cagcgattaa accagacgga cgattcatct acttaagtgc cacacctgat aaaaagttac
    23941 taaaggaaat caagcagacg tttgacatcc acaaacttgc tttacgcttt catcggcgtt
    24001 tgcttcctga acccgtcttg gttttcatga ataattggcg acaaaaatgc aagaacaaga
    24061 agaagataaa atctttggtg cggatcattc atcaattaat taaagaaaac catatccttt
    24121 tattttgtcc aagtatcacg atgatgcatc gtttgaaagc cacacttcaa gaggctcttc
    24181 cgagatatga gatcacagat gtttcttcaa gagacaatca gcgggcggaa aaagtccaga
    24241 aaatgcgaga agcaaaatat gacatactgt taaccacgat gatccttgaa cgtggagtca
    24301 catttgaaag aatatccgtt atcgttttag gagcggatca tccggttttt actaaatctt
    24361 ctttggtaca aatcgcagga agagccgatc gtaaagggcc ttatacgaac agtcaagtat
    24421 actttttcta tgaagaaaaa actgcagcta ttcgaaaagt ttgtcaggaa atcaaagaaa
    24481 tgaatgaaga agggaagaag atgctgtgag gtgcagttgc tgcagtaaag atatcttcca
    24541 aaatttaaca ttcagcgaga ttattggtgt ttcgaaatac agattacctc agacttgttc
    24601 agtttgtcgc gcacaatttc atttattgga aacaagaggc tgttctggct gtcaaaaacc
    24661 aatccatttc aatctagaga gtacgaaaaa gaaagtaaag tctattcccg tttactgcag
    24721 tgactgtcaa gaatggaaga agaaatatcc gacctatgat tttcagcacc gtgccttttt
    24781 tgtgtacgat gaaaatatga aggcctggct caatcaatac aagtttatgg gagatatccg
    24841 actagcaggc acctttgcat atagatggac gcagataaaa aaagaatacc cgacatacat
    24901 ctattgtcca atacctttat ctgaaaaaag gcgaacagaa agaggattca atcaagtaag
    24961 cgagatgttg aaagcagcaa gaattccttt tcagatgcta ctcaaaagag agaagcatct
    25021 gctggctcaa gcaaaaaaca cgcgaaagga aagattggag atgcctcaac cttttgaact
    25081 tgcagtcacc ttttctaaga tacaaaataa aaaaatattg attattgatg atgtctatac
    25141 aacaggacag actcttttcc acgcagcaga ttgtttgcag gtcgcacagc cgaaaatcat
    25201 ccgaaccatt tctttggcga gatgaattag atgtggaaat ttgatagcga tttaatttgc
    25261 aaataaccta gagttcgata taatagaatt aagaagaggg aaagagtggt ccttctcctt
    25321 cttgattgtg gtagacgaaa ggggtaattt ttatgtttag atataatgta cgcggcgaaa
    25381 atatcgaagt aactgaagcg attcgcgact acgttgagaa aaaagtaggc aaactagaac
    25441 gctatttcag tgacgcaccc gatgcaacgg cgcatgtgaa cttaaaagtt tatacagaaa
    25501 aaacagcaaa agtggaagta acgatcccat taccttatct tgtattacgt gccgaagaaa
    25561 cttctccaga tttatacgca agtatcgatt tggttgttga taaattggaa cgccaaatcc
    25621 gcaaattcaa aacaaaaatt aatcgtaaat cccgtgaaac gggcatgaat actgcagatg
    25681 ctgctgcttt attcaatgaa gaggacgaaa acgaaaatga ttctgaatta gatatcgtac
    25741 gtacgaaacg cctttcattg aaaccaatgg atagtgaaga agcagtctta caaatgaaca
    25801 tgctgggaca taacttcttt atttttgaag atgcagaaac aaatggtaca agtatcgttt
    25861 accgccgtaa agatggaaaa tacggactga ttgaaacaga ctgatcagcc ttacttaaat
    25921 aacaacgaaa acttgcagca gctactgaca gctgccgcaa gtttttttga gtttcctcct
    25981 cttttagatg aaatatgaat aaattatgaa gttcttactt taatacgctt aagatatttc
    26041 aattatctat aactaatgat aaaatgaaaa gtaaagtcta catagtctgg ataatacaca
    26101 gaaggaaaag gagattacac aacgcatggc caatttgatt cgttcactta tcgaaaacga
    26161 taagaaagaa ctaaaacgtt tagatagcat tgcaaaaaaa gttgaagcat acgctgacca
    26221 aatggcagca ttaactgatg aacaattaca ggcaaaaact gctgaattca agggcagata
    26281 ccaaaaagga gaaacattag atcaattact tccggaagct tttgcagtcg tccgagaagc
    26341 agcaaaacgt gtattaggtt tatatccata ccacgtacaa ttgatgggtg gaatcgtgct
    26401 tcacgatgga aatatccctg aaatgcgtac tggtgaaggt aaaaccttga ccgcaacgat
    26461 gccagtatat ctaaatgctc tgactggcga aggggtacac gtagttaccg tcaatgaata
    26521 tttagcaacc cgtgactcga cggaaatggg cgaactttat aatttcttag gattaacagt
    26581 ggggttgaat atcaactcaa aatcttcgga tgaaaaacgc gaggcttata attgtgatat
    26641 cacttatagt acgaataatg aattaggatt tgactacttg agagacaata tggtcgttta
    26701 ccgccatcaa atggtacaac gtccgctgaa ttatgccatt gtcgatgaag ttgactcaat
    26761 cttgattgat gaagcgagaa cgccgctgat catttcaggg caagcagaaa aatccacagc
    26821 tttatataca agagcagaca acttcgtgaa acgtttgaaa gaagaagaag attataaaat
    26881 cgatgtacaa tcaaaaacaa tcagtttaac cgaagcaggg atcgaaaaag cagaagaaac
    26941 attcggtttg gataacttat acgacattga aaatactgca ttgacccatc acttggatca
    27001 agcattgaga gccaactata tcatgctttt agatatcgat tatgttgtcc aagacggaaa
    27061 agtactgatt gttgaccagt tcactggtcg gatcatggat ggacgccgtt attcagatgg
    27121 acttcaccaa gcaatcgaag ctaaagaagg cgtagagatc gaagacgaaa cgaaaaccat
    27181 ggcgacgatc actttccaga actatttccg tatgtacaag aaacttgcgg gaatgactgg
    27241 tacagctaaa acagaagaag aagaattcag agaaatttat aacatccaag tataccaaat
    27301 tccgacaaat cgtccggtta tccgtgatga tcgtccagat ttgctttacc caacattaca
    27361 aagcaagttc cacgctgtcg tgcaagatat caaagatcgc taccgcaaag gtcaacctgt
    27421 gttagtcgga actgttgcgg ttgaaacatc tgagttatta tcagatatgc tgaatcaaga
    27481 acgtattcct catgaagtgc tgaatgcgaa aaatcacttt aaagaagcag agatcatcat
    27541 gaatgctggt caaaaaggtg ctgtgacgat tgctaccaac atggctggtc ggggaacaga
    27601 tatcaagtta ggacttgggg taagagaact tggtggtcta gcagttatcg ggacagaacg
    27661 acatgaatct cgtcgtatcg ataaccagct tcgaggacgt gcaggacgtc aaggagaccc
    27721 tggtatgtca cagttctact tgtcattgga agatgatttg atgaaacgat tcggttcaga
    27781 acgaatcaaa gctttcttag atcggatgaa gatcggtgag gaagatgctg ttatccaaag
    27841 caagatgctg acgaaacaag tagaatctgc acaaaaacgt gtagaaggaa ataactatga
    27901 cacacgtaaa aacgtcttac aatacgatga tgtgatgcgt gaacaacgtg aagtaatcta
    27961 taaacagcga caagaagtaa tcatggaaga aaaaagctta tccaagccac taatgaacat
    28021 ggtcaaacgg acaatcagtc gtgtagtaga tgcgcataca caattagaga aagaaaattg
    28081 gaatttggaa agtatcatcg attttgccgg cagtactctt gtccatgaag atagcattac
    28141 tcttgcagat atcgaaggaa aaacagctca ggagatcaaa gagtatctaa acgaacgagc
    28201 acaagaagta tttaatacca aagcggctca actaaatggt ccagagcaac tgttggaatt
    28261 tgaaaaagtc gtgatcttgc gtgttgtgga ctctaaatgg acagaccata ttgatgcaat
    28321 ggatcaatta cgtcagtcaa tcggcttacg tgcttatgga cagaacaatc cattagtcga
    28381 atatcaaaca gaaggctata agatgtttga agatatggta ggcgccatcg agtatgaagt
    28441 cactcgaatc tttatgaaag ctgagatccg tcaaaacgta cagcgtgaac aggtttctca
    28501 aggaacagct tctcactcag aagacggaga tgccaaagaa aatagtaata ctagcgcgaa
    28561 aaaacagcca atccatgtga ataaggtcgg aagaaatgat ccttgtcctt gtggaagcgg
    28621 gaaaaaatat aagaactgtc atggaaaaaa tgcaaactaa tttctgacag caggattggt
    28681 gagacaaatt ctttttttaa gaattcgtct caccttcttt ctgttatttg ctgattctgt
    28741 tagactagat ataatgaaaa aagtatagtg aaagaaggaa aaaacatgga aatatccgaa
    28801 attcgaaatc tcctgacaga aatgcaggaa aaggtcacaa gtttcaggag gtctctttga
    28861 cttagatcag ctggaagaag acatcgcgga agcagaagca aaaatggctg aacctggttt
    28921 ttgggatgac agcgaagccg cacaaaaagt gatcagcgaa aataacgcta ataaagaaac
    28981 ctatgaccaa ttccattctc ttgccgaaga actagaagaa atcgaattga tggtggagat
    29041 gctgcaagaa gaacctgatg cagcaatgca agaagaagca gaaacacgta tcaatgcatt
    29101 agatgaaaaa atgaagacgt atgaattatc tatgctgtta gacggacctt atgatcgaaa
    29161 caatgcgatc gtcgaattgc atccaggggc tggcggaacg gagtcccaag actggggcag
    29221 catgctttta cgtatgtata cacgttgggc agagcagcat ggctatcaag tagaaacact
    29281 agattatcaa gcaggtgatg aagcagggat caagagtgtg accctgctga ttaaaggaca
    29341 taacgctttt ggttatctaa aatcagaaaa aggtgttcat cgccttgtac ggatttcgcc
    29401 ttttgactca gcgaaacgac gtcatacttc tttttgttcg gtagatgtga tgccagagct
    29461 tgatgaaacg atcgatatag aaatcaatcc agatgatttg aaaatcgata cgtaccgagc
    29521 aagtggtgca ggtggtcagc acatcaataa aacagaatct gctgtacgga tcactcactt
    29581 gcctacagga acagtagtag ctagtcaggc acaacgctca caattgaaaa accgtgagca
    29641 agcgatgggg atgttaaaag ctaagcttta tcaattagaa gtggaaaaga aagaacaaga
    29701 agcagctgca cttcgaggag aacaaaaaga aatcggctgg ggttcacaga tccgttctta
    29761 tgttttccat ccctattcta tggtaaaaga ccatcggaca aacgtagaga caggaaatgt
    29821 ccaagcagtt atggacggtg accttgattt atttattgac ggctatttaa aaatgcatct
    29881 acaataagaa cccattgtta aattgaaata aaattgtaag attttgcaag catgttgaaa
    29941 cattgccatg ctataatagc taaagactga aaaagatatg gagagaacgc catgattgaa
    30001 atgaaggatg taatgaagaa gtactcaaat ggtacgaccg ctatccgcaa tctctcgatt
    30061 gtcatcgaac aaggagagtt tgtctatgtg gttggtccgt ctggagccgg taaatctaca
    30121 ttcatcaaat tgatgtatcg agaagaaaaa gcttcaaaag gaactttgaa cgtcgcaggt
    30181 catgatctaa tagcaatcaa aaaccgcgaa gttccttatc tacgcagaga aatcggcgtg
    30241 gtgttccaag actataaatt attaccaaga aaaacagtat atgaaaatgt tgcgtacgct
    30301 atgcaagtta tcggcagaaa acctcgagat atcaaaaaac gagtaatgga agtgctggac
    30361 cttgttggtt tgaaacacaa agttcgagtc tttcctagcg aattatccgg tggggaacag
    30421 caacgtgtct ctattgctcg ggcaatcgtg aatacgccta aagtacttat cgcggatgaa
    30481 ccgacaggaa atctggatcc agaaaactca tgggaaatca tgaaattatt agatcggatc
    30541 aatgcccaag gaactactgt cgtgatggct acacataaca gtacgatcgt aaatacgatt
    30601 cggcaccgag taattgctat cgaaaacggc cgcattattc gagatcaagc ggaaggggaa
    30661 tatggatacg atgattagaa cctttttccg ccatttatta gaaagtatca agagtttgaa
    30721 acgtaacggc tggatgacag ttgcttcagt cagtgcggta acgatcacgt tgactttagt
    30781 cggtgtattc atggcagtta tcatgaatgc aactaaatta gctcaggata tagaaggaaa
    30841 cgttgatgta tctgtattcg tagatatcgg tacaaaagac gaagagatga aaaagctaga
    30901 aaaagaattg aatgacttag atcatgttaa aaaagttgaa ttttcaagca aagaacagga
    30961 attgaaaaaa atccaaaacg aaatgggtga ttcctggaaa ttgtttgaag gtgacaataa
    31021 cccgttatat gatgtgtatg tagtaagtgc tactgatcct tcttatacaa aacaagtagc
    31081 agaagaagca gcagatttga agaatgtttc tcgttctgat tacggtggag cttcctcaga
    31141 tcggattttc caaatcgctg gtgcagttcg gacatgggga ttagttgcag cggcattgct
    31201 gctgttcgta gcgatgtttc tcatttcgaa cacgattcgg atcacgattt tgtctagaca
    31261 gcgtgaaatt caaatcatgc gtttagtcgg tgcaaaaaat ggatatattc gctggccgtt
    31321 tttccttgaa ggtggctgga tcggtctgat cggtgcgatt cttccgattt tgatcatcac
    31381 atttggttat gcatggtttt tccaattgat caatccatca ttattacgtt cccattattc
    31441 attgattcat ccacaaaata tcgtttggaa aatcaatctt ttaatggtcg gcattggtgt
    31501 gatcattggt tcacttggct cgatcatttc tatgagacgt ttcttgaaat tctaatacca
    31561 caatataaat aaaatgagaa agttggacag tttcgttcca gctttctttt tttattggct
    31621 aggagaatag aaggaagcgg cttttttccc tttactttaa aaaacaactt gggtattctt
    31681 aagttggtag aagaagaggt gaagccgtga aaattatcgt tgacaatttt tttacgatcc
    31741 ttttgattat gaatattttg ctttccttta tcatcgtttt tcgagaaaga aaggaaactg
    31801 ctcaaacatg ggcatggctg ctcgttttga tgttcattcc agttgtagga tttattttat
    31861 atattttttt aggaagaggg atttccaaag agaaaatctt tgatttgaag atccaaggga
    31921 aaatcggtaa gaacctggaa attgaagaag acaaacaggc attgatgcga gggctttatc
    31981 ctcatccgcc tactggacaa gtagatgtga aacaattgat ctatatgctc acggtatttg
    32041 aaagtacgct ctatacgact aaaaatgaaa tcactttatt tacagatggt cgtgaaaaat
    32101 tcgatgcgct gattgaagat atcaagcagg cagaagacca tatccacttc cagtattata
    32161 tttatcgcag tgatgcatta ggtgaagaag ttcgagatgc attgacagag gcggcaagac
    32221 gaggggtaaa agtcagagtc cttcttgatg catggggttc cacacaagtt tcttccagct
    32281 tttttcagaa tttgaaaaaa gcaggcggag aagtcgcatt tttctttcct ctatttgttc
    32341 cgtatattaa tcctcggatc aattatcgca atcatcgtaa aatcgtagtt attgatggga
    32401 aaatcggata tacaggtggg ttcaatgttg gaaatgaata tctaggattg gtaaagaaat
    32461 ttggttattg gcgagacaat catttgcgta tttacggaga agcggtgtat accttgcaaa
    32521 accgtttttt gatggactgg aattctcagc atacaaaaga agtaggctat tctcccaaat
    32581 tttttccgtc cattcacact actggtgata tagctgtt
//
LOCUS       DACDYI010000027        31990 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.027, whole genome shotgun sequence.
ACCESSION   DACDYI010000027 DACDYI010000000
VERSION     DACDYI010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.027"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            610..1008
                     /locus_tag="IWM52_001230"
     CDS             610..1008
                     /locus_tag="IWM52_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973490.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
     gene            1014..2129
                     /locus_tag="IWM52_001231"
     CDS             1014..2129
                     /locus_tag="IWM52_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973491.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            2252..2574
                     /locus_tag="IWM52_001232"
                     /pseudo
     CDS             2252..2574
                     /locus_tag="IWM52_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3560..3793
                     /locus_tag="IWM52_001233"
     CDS             3560..3793
                     /locus_tag="IWM52_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973492.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            3796..4098
                     /locus_tag="IWM52_001234"
     CDS             3796..4098
                     /locus_tag="IWM52_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973493.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            4124..4474
                     /locus_tag="IWM52_001235"
     CDS             4124..4474
                     /locus_tag="IWM52_001235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973494.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            4488..4670
                     /locus_tag="IWM52_001236"
     CDS             4488..4670
                     /locus_tag="IWM52_001236"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973495.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            4651..4857
                     /locus_tag="IWM52_001237"
     CDS             4651..4857
                     /locus_tag="IWM52_001237"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973496.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            4884..5201
                     /locus_tag="IWM52_001238"
     CDS             4884..5201
                     /locus_tag="IWM52_001238"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973497.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(5295..5717)
                     /locus_tag="IWM52_001239"
     CDS             complement(5295..5717)
                     /locus_tag="IWM52_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973498.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(5780..6025)
                     /locus_tag="IWM52_001240"
     CDS             complement(5780..6025)
                     /locus_tag="IWM52_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973499.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            6170..7009
                     /locus_tag="IWM52_001241"
     CDS             6170..7009
                     /locus_tag="IWM52_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9973500.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            7160..10177
                     /locus_tag="IWM52_001242"
     CDS             7160..10177
                     /locus_tag="IWM52_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9973501.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            10241..11704
                     /locus_tag="IWM52_001243"
     CDS             10241..11704
                     /locus_tag="IWM52_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP9973502.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            12171..12551
                     /locus_tag="IWM52_001244"
     CDS             12171..12551
                     /locus_tag="IWM52_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAP9973503.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
     gene            12548..12817
                     /locus_tag="IWM52_001245"
     CDS             12548..12817
                     /locus_tag="IWM52_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973504.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            12817..13737
                     /locus_tag="IWM52_001246"
     CDS             12817..13737
                     /locus_tag="IWM52_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973505.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            13759..14631
                     /locus_tag="IWM52_001247"
     CDS             13759..14631
                     /locus_tag="IWM52_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9973506.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            14650..15822
                     /locus_tag="IWM52_001248"
     CDS             14650..15822
                     /locus_tag="IWM52_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP9973507.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            15833..16483
                     /locus_tag="IWM52_001249"
     CDS             15833..16483
                     /locus_tag="IWM52_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973508.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            16499..16846
                     /locus_tag="IWM52_001250"
     CDS             16499..16846
                     /locus_tag="IWM52_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973509.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAFLEGASVVVHEVVIVDKI
                     PKLLLERYGISHE"
     gene            16839..17837
                     /locus_tag="IWM52_001251"
     CDS             16839..17837
                     /locus_tag="IWM52_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9973510.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
     gene            17861..18145
                     /locus_tag="IWM52_001252"
     CDS             17861..18145
                     /locus_tag="IWM52_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973511.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            18226..18624
                     /locus_tag="IWM52_001253"
     CDS             18226..18624
                     /locus_tag="IWM52_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9973512.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            18634..21177
                     /locus_tag="IWM52_001254"
     CDS             18634..21177
                     /locus_tag="IWM52_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HAP9973513.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            21199..23541
                     /locus_tag="IWM52_001255"
     CDS             21199..23541
                     /locus_tag="IWM52_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973514.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            23557..25731
                     /locus_tag="IWM52_001256"
     CDS             23557..25731
                     /locus_tag="IWM52_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HAP9973515.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            25721..26143
                     /locus_tag="IWM52_001257"
     CDS             25721..26143
                     /locus_tag="IWM52_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP9973516.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTFLQR
                     YHVKSVPSFLVIHDRNVKKIERKTFFFEKTLNQFLPNF"
     gene            26962..27873
                     /locus_tag="IWM52_001258"
     CDS             26962..27873
                     /locus_tag="IWM52_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973517.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            28360..28626
                     /locus_tag="IWM52_001259"
     CDS             28360..28626
                     /locus_tag="IWM52_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973518.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            28698..29039
                     /locus_tag="IWM52_001260"
     CDS             28698..29039
                     /locus_tag="IWM52_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973519.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            29094..29513
                     /gene="ssb"
                     /locus_tag="IWM52_001261"
     CDS             29094..29513
                     /gene="ssb"
                     /locus_tag="IWM52_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9973520.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            29590..29886
                     /locus_tag="IWM52_001262"
     CDS             29590..29886
                     /locus_tag="IWM52_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973521.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            29901..30332
                     /locus_tag="IWM52_001263"
     CDS             29901..30332
                     /locus_tag="IWM52_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973522.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            30514..30819
                     /locus_tag="IWM52_001264"
     CDS             30514..30819
                     /locus_tag="IWM52_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973523.1"
                     /translation="MKLDMPGRYKLLRVRLFLIKLTNLRMYGSITDLYSKDIRLRMQR
                     TLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKRFTEEIEQLAELNIRYF"
     gene            31223..31711
                     /locus_tag="IWM52_001265"
     CDS             31223..31711
                     /locus_tag="IWM52_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP9973524.1"
                     /translation="MKKTIFVLAIFICFGLLLVGYLLFNSNEMRTISTNDLLKKSELK
                     DKKKESLAFIMFTQEECEYCIAINPIIDSVNKETLNKIKKYDISVDFQSKQKCKIKFV
                     PTIVAYSKNKEVGRIDNELDNLYRKKQDGSIVIDKKKTKTLLIDWMKKMDKQGEYLYG
                     ED"
BASE COUNT        11597 a         4916 c         6662 g         8815 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtc
      121 attaatgtca atttttaaat aaaattaata tgtctcttta agtattaaac tatcactctt
      181 tatcctatga taaatcaatg ttttttaata ttagtagtga tatattaaca tcaaataata
      241 ttaactaaga aagataagga aagatagaaa tagggaaaaa aatcggaaaa aatgttggca
      301 aaaattttga taaatttact ataaaaataa cgtcgatgct ttaataagta tcgacgcttt
      361 ttacattata ggaaatatat tatgaatggt gtttaaaaat taatagaatc aaacttttgg
      421 gcacatttca cttgatcttt ttctctttaa aatgaataaa tgaaggcgaa gattctacaa
      481 tctaaatatt agtaagtgtt tagtttttac ccgaaacgta ttttagaaat taaatatcaa
      541 caagaagaaa ctctgtgtaa ttcgatttat acagagcttt ttttgcgtgt ttttctagga
      601 ggaaaaaata tggtaaaaaa caaatttcgc gcaacagatg tgctgacaca agcaactttt
      661 catatgccaa aactattctt tataaaatcg tcaaaatatt atgcgatgac tgcacaagca
      721 aaatacgcct atagcttact gaatgagtac ttagaagaat ccttaattga aaaacggatt
      781 gatgaaaaga aacatgtcta tatttcttat accaatcaag aattagccat tttactcaat
      841 gtgtctgaag aagaaattga agaaatcaaa aatgaattag aagattacga cttactaaca
      901 attgaattcg gtcgaattta tctaaaagaa ttgattgcta caatggaaga tgttcgtgtc
      961 taccaacacc aaaattcttt cttaatatat ggacaagatg aggtgtaata acgatggcaa
     1021 caaaaagatt taccgcaact cgtaaataca atgaattata ctatcaattt ccaaaggtat
     1081 ttattacaag tgaaaaatat cgctcattgt ctgataaagc aaaaatcgcg tatatggttt
     1141 ttcggtcacg cttagattta gccattcaac gaaatcaagt agatagtcaa ggttttgttt
     1201 attttgaatt tacggaacaa gaattagcca atgcattgaa ttgttgcaga cgttctgtta
     1261 atacaatcaa aaaagaattg aaagaatatg agctactaga aatagaggat atgggatttg
     1321 atcaacaaac aggaaaaaga aacaaagctc ggttgtatct aggagaatta gatacgacag
     1381 aaaatgatgt gtattcatta ggtcctgttg actcaaccag tgtagattct gcacaaggaa
     1441 aagaagagga ggaatctgtt gataatttag cgattttcga tgattttgac gaaaaaaatc
     1501 catgtgaaaa ttctgcacaa ttatttaata attcttctaa caatctagat acagaaaatc
     1561 tagatacaaa aatcgatacg actagtcctg ttgaaatggc tgatgattgg caaaaagagt
     1621 ttattgctca ttacagcgaa agtacgtttg ttccgaaaga tgttttgaca tggattgttc
     1681 gcagttcagc tgaatggcgt gaagcgaaag agcagctaaa catgatatac gacagtaaac
     1741 gaattataga aaagcggcaa cgaaagtata atcaaacaca tggtttatct gatggaagcg
     1801 agtatcgaat tttaggggaa acatttgcag aagatttaag caaaacgtat caacggtgta
     1861 ttcaaaatga aaaaattgct cgtcatgaag gaaaagcaat tcagaatcga aaaggatttt
     1921 attttagagc gctattgaat ttttgggaat gttgtgtaag agaattgaat ctagatccaa
     1981 tggcaacaaa actatcagat caagcagaat attcattact tcaagccaag cgttcttgta
     2041 gttatgaaga cctggaaacg cagatttatg ggctgtcaca attgcccaaa atttcgatgc
     2101 ataattggtt ggaggaattg gacacatgaa aatgacccct aatcacttac gtcatatttg
     2161 ttgtgaagta acattgttta atcggcaacg ttggaagtta tcgaatcgga ttgttcgaag
     2221 aatcattgta gattcaacag ggaaaagcac agtgattttg acaaaaaaat tagcaacaaa
     2281 acaagatttc caactgtttt tttctcaagt gaaaactatt ttttttgatg ggaaaattat
     2341 tgcgaatcgt ggttgttcga accaggattt gatggtttca aatattgttt tctcacgaat
     2401 gaaacgccga caagacctat taattcaggc acgaaaagcg tataaagtat tcaaaaagca
     2461 agaaaagcag tccagaggaa aacaaaacga gcaaataatg aataaaaaat gtatcgaagt
     2521 attgaacggc aagaacgacc acagcgacac caaaaagaaa gagggaagac atgaaaacta
     2581 tccgatttta cttgtttgcc ccaattgttt ttcctgtggt tttgttgggc gtattggttt
     2641 tagaacttgg aattaaacta acaactattt atcgacagtt aaaaaaataa aacggaaaga
     2701 aactagcctt ttttgtttgt tagtttttat ttgagctaac aaacaaagta taaaatagcg
     2761 agtagcgagc ggataatcgc tatcgaaaaa agaataagta agcacacgga cacgaaacgt
     2821 aaatgctttt caggaacatt tgatgatgaa aggaaaagct agctggtgga aggaatccct
     2881 tgaacgaggg gagtagaaat actcaagtac aaacaggtac tttgaacaga agcgatacac
     2941 gtaaacctac ttatttcttt tgggagatct ttgaaaattg gatacaaaca ggactttcta
     3001 agttaatcag cgtgtctaac agggacttag gaaggataaa ccacctaaaa aagtatggtg
     3061 aatctcaagc gatacgtatg gatgggtgtg gcacagaaca aaaaaaggtt atttgcgtgg
     3121 atcaggtagt atgaaaaaca tatgatgacg agtacaatcg tccgctcgtt tttcaaggga
     3181 gcaaaggcac ttttttagag tttggggttg ctgacgctag aaaccctgcg aaagaaaaaa
     3241 tctgttaaat cgttgatgat tgatgaaaac tagtaaacaa aagaggctag ttttgaaaaa
     3301 gaaaggaata ctatatgaac aagaaaaatg agcagtgcag tatcaatcga ttgatgaaaa
     3361 aactaattgt gacaatggat gaaaaaaagt atctggcgtt ttaaaaggtg tgaccaaata
     3421 tgaactgtat gtggaacaag taaaagacgg aaataaaaca gggcaacatt ttgttttgtt
     3481 taagcatgca gtgaagtata ttcgttggca ggaataggac aggaaaaagt tttggataaa
     3541 aaagaatagg agtgaggaaa tgacaattag cgtaggaaat aaagtgaagt atatcggaac
     3601 agcaattcca cggtatacaa acaaattagt agtagtgaaa aacatattgg taaacggtct
     3661 gattcttgaa tatccgaaag aggacaggag aaaagttgta gttgaaggga atggtacttg
     3721 gaatatgaat tcatttattt gtggatttac tgaagtggag gtaacaaatg ataatgaaaa
     3781 taggagtgga tagaaatgtt gtctaaacaa gaaaagaagt atattcgaca gaaaatgtat
     3841 gcaatgatag gcaatcgagc aatcactgaa ttaagttcag aagagctgtt acaagtacaa
     3901 caattggccc aaataatcgg ttcgaattct atttttgatt cgaacccatt gcctgaaatg
     3961 acgttagaga cgctaactgc taaaagatat aaagaacttc gtgcaatagg ttatcgaatg
     4021 attgatattc gacgagcatt aaggattagc gatactaaat ttagagaatg gcgtaccagt
     4081 aacggcttag ctaattgaga gaggaatgaa agaggaacta acaatgaaaa aaagttataa
     4141 agtatttgag aacaatgaaa taaaagaaca agaagcaatt attctatctg ctcaaacacc
     4201 agaagaagca ttagaacagg cactcccttt cttggtagaa tcccaaaaca tagttgtgga
     4261 agaattaccg gaatttgatt caaaaatagt tttttcgttc aattgtaaga aagaatcaga
     4321 aatcagtgtt tctccagaat tttattgtcg cagtgatata tcagttgaag gtctggtaat
     4381 gtgtgcgagt gcttttattg aaatttttgc gaaccatttg caaatttcgg tgaaggagtt
     4441 attagaccta ttacaagaaa taacaaacga gtaaagaagg aaacaaaatg acaaaatgtg
     4501 aaagagtaag tgtaaccatt agtttcattg ctttattatt aagtgtgatc agcttgttga
     4561 atacagcgat ttttatagga caagtggaga aacaaaaaga aatctcacta tctgaatggc
     4621 ggtatgaaga agtgaatgag ggaatgaata atgaattggt tgaaaaatga agcagaatta
     4681 tatgtttttc atgaagaaat gcaggaaaaa catttagtac cgaatgtaag aaattacctt
     4741 ttttggttag aagaaaaaaa cgacgctcaa gtttttggaa agagtgcaca aagccctaag
     4801 ataaaactcc aattaactag taaacaatgg aatgaacatg agtggtatat ctattgaagt
     4861 gagtcaaagg aggaaaagtt aagatgccag taaaaggaac gagtaatttt gattttgaaa
     4921 ttttttatgt agatgacgaa atttgtggtg tatttaacaa acaaaagtac accaaagaac
     4981 aagcaattga agcatggaaa aatgaattat ttgatccaga agaagaaatt tcattcgttg
     5041 tagatgaatc ctttgttggt tggtatcatg gtaatgctga ggaggaagaa gttcattatg
     5101 caggttggtg gttagaagaa ggggagcctc gagagcaatc tgtgcctgta tgggtcatta
     5161 gcgaaaatcc cttggaagaa attgagaaat tagaaaaata gtttcttcaa aaatgttgat
     5221 attttcgaag cgtttttata aaatgattat gtgataaaaa taacaagagg gtttttcctc
     5281 ttgctattag aaacttattt cagtgagtaa gcaaagtatt gttcatttac tttagccaaa
     5341 ccaaacgtat ccccaatctt taacttgtta ggggtatatt gaatgtcagg aatggagaat
     5401 aaattttctt tggtatttgt tactttgata gaaaattcaa ggtcagttcc ttctttagga
     5461 gtaataaacg caatattttt cttttcaaat aagacagtag taatttgaga agaaccaaga
     5521 tcaattaaat cttgtccatg cgtttctttt aaactattta gtgaaggata gacggaatga
     5581 tcgctagatt gacttgtagt agtcgattga accgtatttt gtggctgtgt ttctgttttg
     5641 ttggatacag aaaaagcaat aatagcgagt acaatcagta cgattgaacc aactgaaaca
     5701 atgtatttct tattcataga acctccgttg taaaagtgtt tattattgtt ttacttgtaa
     5761 atccaagaca gaaaacgcct cattgacgtt tctgtctttt ttcttcgagc tgttttgcgt
     5821 atttttcaat atcttctctt agatataagc gaatgatgga aggaccaggt tcgtcgccta
     5881 tttctagaaa tggtttgata ttatttctac gtaaagagct agaaaaagca tcatagtttt
     5941 gtccagtgat tttggcagct tgtttacgat taagtaaatt attttgagcc caatcatgta
     6001 tttcatttaa attattaagt tgcatgtgtg acattccttt tagttatttt agtaccaaag
     6061 ttagtatatc aaaaaataaa aaaatctaca aaaaatatat cgactgatag acataagttt
     6121 ggtactatgt ttttgtaaat aaatttattc aaatcggagg cgaaaaacaa tgaaaatgtt
     6181 gaaagtattg gcactaggaa tcgtagtcgg aggagttagt cttgctgtgg gaacgaatgc
     6241 tgaagcagca gaaaacaaag tttggacacc tcgttcggcg gaacaaatca aagcagaaat
     6301 taaagggaat gaatacacaa ttgtctgggg agatacccta agcgcaatca gtcaagcaac
     6361 aaatattact gtccaaaaat tagcggacat gaataagatt acaaatgtgg atttaatcta
     6421 tgcaggaaat aaattagtat ttaatggcaa tgtagcaagt gtacaaaaca gcgcaggtga
     6481 aactgtggct caaacagtga ttcaagagaa agacaaagtt gatccgaatc aaccagtagg
     6541 acaaggaacg acttcaaata cacaaacgac accaaataca acgacaccaa caacaccagc
     6601 tggagatcaa acaggaacaa ctggtggtac aacaacgcca agtacaccag aaaacaatgg
     6661 tggcactact caaacaccta cacaaccagg taatggtgga aatactggag gagaaacaac
     6721 tccttctaac ccaagcacag gtggaggaac aacaacgcct gaagaaccaa gcaatcctgg
     6781 tggaggcgat caaggcggaa atggtggcac aacgacacct gaagagccaa caattgtagt
     6841 tggagagatt ggtaatagtg gaaaggtatt taacactgta gatgaagcct atgattgggc
     6901 taatgcagag gttagcgatc caaattcgca gtggtacaac aaaggtttta tggtggatgt
     6961 tattatttac agtgacgggt cacaaaaaca cactgtagat ttctactaaa aataaaagaa
     7021 aagtaagtgg ttgatgaaga ctagaattat aagttctagt ctttttttgt tttgaggagg
     7081 ataaaaatgt ttcggtgtcg aagtccataa gaaattactt tagaagactg aaaaataaaa
     7141 tgaaaggaac gaaaaaaata tgtttaaaag aatggcaact tggttggcaa ttttcgttac
     7201 atttctcaat cccatgttag gatctgtaac ggcatttgca gaaacgataa atgaatcaaa
     7261 ttctactgaa gaagtaatga agactgaaga atcaaaaagt agtgaagaag cgatcagtac
     7321 tgaaaccaca agtgaaacaa cagaagagac aactacgcag gacacgacag aagatgtttc
     7381 tccgcccaat gtaatgactc aagaggtccc tgaaggttat gagaaatact ttataaccat
     7441 tgaagatatt caaaaagcga cttctttttc tgaacaagaa cgattggcgc aagaatctga
     7501 agaatggcaa gcaatcttgg aaaagaatgc acagacacgt gctaccaatc taacattgac
     7561 tgaaaatggt cgtaaagatg tgaatatgac tcgaattact cctgaaggaa caatgtatac
     7621 tcatgattac atggttcgct ggcaaattga cggagaagat gttttttgca ttcaagaagg
     7681 cgcaatcacg agtgcgggta ttcaatatga tgtgacacca ctcgaaaatt taattacgac
     7741 aagtgctatt agaaaaaaga tttctttaat tggttatttt ggctactatt ctagtggttc
     7801 aatggataac tatgtattag cacaaacata tatttgggaa gttttaggtg gttcatttgt
     7861 taattatgga actattggac aatctaatta tgaagctttc aaacagactg tagatagaaa
     7921 aataaacaat ttacttgaag atccttcttt tgatagccaa acagtgacgg ttaaagtagg
     7981 ggaagaagta gaagttaagg atacaaaagg tgtgttttct caatgggatg ggtcggttgt
     8041 tgaaaacaca gctaacgttc aagtaagaaa atcaggaaat agtttgttta taaaagcaaa
     8101 tcaaaactca aatgacggac gaatttctgt ttcttcactg gatccaaatt ctagaggggt
     8161 aggttcaagt tttgcctatg tagatcctgt tgctcaaaat ctaggaaagc tttatgtgcc
     8221 agatcctaat agagctggac taactattaa agttctaaaa aatggcgact tcaaactaac
     8281 caaactagac gctgtcacag ggaaaaaagt tccaaatgtg acgtatgaaa tcaaacgtca
     8341 tgatacggga gaaacaaaac aatatacaac ggatgccaaa ggggaaatcg ccttaaaaga
     8401 aatccctgca ttctatgaaa aagacggaaa atatgttcaa aataaattgg atgtcaaaga
     8461 agtcaaagca ccagctgggt atattctaga caaacaaact catacgatta cgattgaacc
     8521 aaacaaagaa gtgacgttaa ctgcaaaaaa tgacttacaa cagttcattc cagaaggaaa
     8581 gaaagtcaaa gaagtctata atgcggaaga atcctggaat caaggcaaac ctgtttatga
     8641 taccgttcct gcagaaaata tcgaatttga tattatcgcc aaaacagata ttactgcgcc
     8701 agataaagaa acagtccttg tgaaaaaagg agaagtcttg gattcagtaa aaactgacaa
     8761 agacggacac tggaaggcaa acaaaaaatt ctacaatggc gatcaaaaca aataccaatt
     8821 gattgaaaaa aatgtgccag acaactaccg tcaaccttct gaagaacaaa ccacgttctc
     8881 tgtggcctat ggcgataaca cgacgcctgt gcttacgttt gaattagatg tgattgacaa
     8941 cttgttaaaa acgggtgaag tagacttcaa taaagtcgat tcaatcaatc atctacaatt
     9001 accaggagcg aaattcctga ttgaaggtat gactgcacat aataaaaatg tgtcctttgt
     9061 gtatgaaaca aaagaagaac cagaaaaact aaaattacca gacggtacgt ataaatttac
     9121 ggaaattgtg ttccctgagc attatgggca agcgtcagga caaacagaaa cgattttagt
     9181 aacggtcaaa gacggagaag tacaaccaat tactgtgaaa aacgaagaaa tcgtggttcc
     9241 agaagaagac caaccagaaa tttctacttt gtttgtgaca acgacaggtg gaaaagattt
     9301 caacccgaaa aaagaccaag aattagtgga taaagtgtcc caagtattcc ctaaatctgc
     9361 ggttgggaca gagaaatatt gggtgacgca attccataaa attactgccg atggaaaagt
     9421 cactgtatta gatacggtaa aagaaacacg aaaagtgacc gatgaaaaag aaacatttga
     9481 tgtgacgttc aactacaaag cagacatggt ttcagacggt gacacgattg tcgcaacgca
     9541 cattgtctat aacgacaaag aagaggaaga agaattcgct cgtcattttg atttaaccaa
     9601 taaagatcaa acagtgactg cgaaagaacc aaaaatcgaa acacgcttta ttacagtgaa
     9661 tggcgaccaa acatttgatc cagacaaaga ccaagagttg atcgatcatg tatcgcaaga
     9721 atttccagaa tctacggtag gggaaaccaa atactgggtg actcaattcc ataagattga
     9781 taaaaatgga aaagatacag tcgttggtac ggtagaaact gcacgtaaag tttctaaagc
     9841 gtctgaagag tttaaagtat cctttgacta caaagcaggc acgttaaaag acggggagaa
     9901 attggtcgca actcatattg tctataagga taaagaccat aaagaggaag aagcacgtca
     9961 ctttgatttg aagaatgaaa aacaaacatt gacagcaaaa ggcattaaaa aagaaacgcc
    10021 gacaacccct gtcaaacaaa cgccgacgag cacaccaaaa caaagcttac caaaaacagg
    10081 agaaacagtg aacgatgtgt taattgcatt gggactgttt ctttttgttg gtgtgggggc
    10141 tgttctggtt cgtcgtaaaa tgaaacaaag caattaaagt atgactagag acagcaatca
    10201 gctgtctcta gatgattgat tgaagaatgg aggcaatctt gtgaaaaaaa cgagtaaatg
    10261 tttatctgct ttgttattag taggtcaagt atttgggtat acgccagcgg ttttggctaa
    10321 tgaacaaggc aacaatcaac tgacaaattc gcaatctgca acgatcactg aagaaacgac
    10381 aacaaattcc actggagaag aaaatcaacc agaaacaccg actgaagata caatagatac
    10441 accagataat ccagaaatga acgctccacc aacaggttct gaagaacaac cagaaacacc
    10501 gaatcaacca gacaaaccag aagacaatca acaacctgag gaggaaccgt cagaagaacc
    10561 aaatgagcaa ccttctgatg aaagctcaca gactcctcaa aaaccaagta ccgaaacaga
    10621 agcggaaccg ccaacaaaaa cggaacgccc acaaaaggca cctgaacaga gtgtgactgt
    10681 acctatgaat gacgtaccgc aggcacaatc aatcggttca gaagagacaa aaaactacca
    10741 ccaagcacca agcgcaccga tacaagaatt agtcccttcc agcacacaag ataacgaagg
    10801 gactattcat tttgaaaaag acgcgtctgt agaaacgttt atccgtaaaa tcggtgaatc
    10861 tgctcgtaaa attggacaag aaaatgattt atacgcttct gttatgatcg cgcaagccat
    10921 tttagaatcc gcaagcggac aaagtcaatt ggctcaggca ccaaactaca atctatttgg
    10981 aatcaaaggc acacataacg gaaagggcgt ctcttttgcg acacaagaag atttaggcaa
    11041 tgggacactt tatacgaccc aagcaacttt tcggcaatat gagagctatg aagaaagctt
    11101 aaaagactat gccaaactgt taaaagaagg cttagttgga aatacctctt tttatgatgg
    11161 tgtttggaaa agtaaggcaa aaacctatca ggaagcgact aaatttttaa caggaagata
    11221 cgcaacagac actagctacg ataaaaaatt aaatgggtta atcgaaacct atgacttaat
    11281 caaatatgat aaagaagtag ctggtccaca actaagcaaa gaaggctata tggtacctct
    11341 aaaaaattat tcaatttcta gttccttcgg ttggcgagga gaagaatttc atagaggatt
    11401 agacatggca gccaatcaag gagaaccaat ctatgcgagc aaggcaggaa cggtcataga
    11461 agcttcgtat catactttgt caggaaatta tgtcacaatt gagcatgaag acggaagtgt
    11521 ggctctttat gcgcaccaac aggaatattg cgtgaacgta ggacaaacag ttgaacaagg
    11581 acaaattatt ggttatgtgg gttcaacagg aaatagtaca ggaccacatt tacatttaga
    11641 acttgcctta gatggtagtt taacaaaaga caaattaatc aacccagaaa cagtcttatt
    11701 ctaaagaata aggctgtttc tgataaggag tggtatagaa aatgtggtta ctttttaaat
    11761 atttgatata tttgtatctc tcattttcgt tttttaattt gaaatctcgt atctatctag
    11821 aacgtagttt attaaatata gtattaggaa tattttttat cgtttgggtc ttcattgact
    11881 tatttcttta ctcaaacgat ttttatttta tttataaaga atttaaaaat cagtaattgc
    11941 ttggagagtt aacatatatt gcataaaaag gcaaagtatg tacatggtat ttatcgtgtt
    12001 catactttgc ctttttatgg cgaaattttt tagaaaggag gaaaaaattt tgttatttgt
    12061 aagtggattt ctagctggtg gtgtattggc cacctttttt atggcaagtt tcattgttag
    12121 taaaacagaa gaaactagaa tcaaaaataa acattagaaa ggacaaagat atgatcacaa
    12181 attataaaaa attttggcag aatagcttta attacaaagg gaaagccaca agaaaagagt
    12241 attggctcgt tgcgttaatg aatgccctca ttgggtttgg cctaggctta gcgattggac
    12301 tagcaggagc gttgaatgct tctgaaacaa taattggttg tttgataggc gtattgattg
    12361 tgtattgttt cgtagttctt gtcccgtctc tttctctcag cgttcgacgt ttacgagacg
    12421 caggttttca ttgggcattt atttttttct attttgtccc aacgattggc tctcttgtgt
    12481 tactggttct ttattgctta ccgacaaaag agtcaataga agataaacag gtacaggagg
    12541 aaaccagatg attatagtaa caggaattgt tttattaggg ataggcgttt tatttggcta
    12601 tcatttcgcc tattttccaa agattggcga atggcaacaa agacaagcga tccaaaaata
    12661 ttttcaagtc aaaggcaacg aatataccta ttatgctgaa gatgaggata gctatatcgt
    12721 cagtttgaac aatcaagaat atcgggtgaa attttcttta aataacccta ttcaagtggt
    12781 gtatgcggta gaagtggtgg aagaagtgtt tgactaatga tgatgattcg atttacagaa
    12841 gttgataaca tgtctttact aacagataaa aaaattgcat tagataaggc atttattcaa
    12901 ttgaaaagag aaaatgaaga gctattggca cgagcggaaa ttggctggtg taccttggaa
    12961 atctacacag aaaaccatga aattccagta tttgaaggct cgtatgattt tccagtagaa
    13021 gagtttcatt tgatttcatt gattgaacaa tctttaacag aaaatgggca attaagtgaa
    13081 gaagcacaaa cctttattca aaccttaaat tccgaattac cgaagtttta tcaagtaaag
    13141 aaagaccaaa agaacgaacg tcgaccaaca aaaatcaaaa aggaaaagaa aacaccactt
    13201 tctgtagaaa cagcgtttac acaaagcaac ggctctcttc agaagaaaaa aatgtctttt
    13261 caatggcttt tgtttggatt tatcgtcgtt gtatcttgtg gtctttttat tatgcaactt
    13321 tcaacgcaaa gagagattga acaactaacg aaaaaggagc accagttaca agaacgcatt
    13381 gcatttgcgg aagaacttgc gacaaaacaa ccacaaattg atacatttag tcgttatttt
    13441 attacgaatt attattcggg tgaaatggca gaagaacggt ataggaaaaa gcttcaatcg
    13501 tttctaacgg aaaaagttgc gaaacaaatc agtcctcaaa aggaaaaagt caaaagtatt
    13561 ttatcctggg aaatgaagaa agaaggcaag cattggcaaa ttgcgtatgt cattacattg
    13621 gaaaccgaga aaaagacaca agaaacaaaa aaagtgtctt ttcaattgga agaacaggaa
    13681 aaaaattatc tagtttccac gcttcccgaa ctagatgatt ttgaaataaa tcaataaata
    13741 agagaagagg aaaaacacat gaaaaaaatg attttaggaa gtttatttag tgcaactgta
    13801 ttgctaggta tggtaggaac cagtgcgtat gcagatacta cgtcaccagg tgatccaagt
    13861 gtaccaccga cagaaattat tccaccaacg gaagaaacac ctgaagttcc tgaagaacct
    13921 gttttaccac caacagatgg aggagaagaa accccagaaa ttcccgaaat accagaaact
    13981 cctgaaaccc cagagattcc accaacggaa gaaaatccag atacaggtgg aggcacagaa
    14041 acgcctgaag taccagaaaa tccagatact ggcggagggg aaggaacacc tgaagttcca
    14101 cccgttgagg aaaacccagg aaatggtggt aatacagaga caccagaaaa gccgtcgaat
    14161 ccaggaggca atgaaggaaa tacgactcct ccaacgacac cacctaccaa taatgggggg
    14221 acgaacacta cgcctccagt acaggacgtc gtagtaaaac cagatggaag tattggcagc
    14281 caacaaacag gtggacaaaa tgttcaaatt gaaaccaata atgcgtctga attaacccat
    14341 attccaacac caagtacacc aattgaaaca gcttcaggag aaaaaattgt ctccgtcgtt
    14401 gatggtgtcg cttataaaga atcaggaaat ggcttgacgc ccatttcaga caaggtcgaa
    14461 caacttccaa gcggaaatat tgcggtgaaa gggtctgatg gaaaaatgaa agtattgcct
    14521 aagactggcg aagaagtgtc tctcgttgcg acagcacttg gtggattatt ggcttcagga
    14581 agcggttttg ctttttggcg caaaagaaaa gcaacacaaa aaatgaacta agcgaatgaa
    14641 ggatcaaaca tgaagaaaaa acttctttta tggtgtgggt tcggttgtct acccacacta
    14701 ttactatgta tgtttggttt tctgtttatg atacttttga ttagtagtgg ttctgaagag
    14761 gaagaaatcg actgtagtcc aaccgaaatt gtcgcaagta ctatctcatt aacgaaccaa
    14821 ggcatggaga caaacgggct tcaaattgca aaggaagtac aaaaacatat tccaaatacg
    14881 acattagagg gtctagcggg tatgttaggc aattttcaac aagagtcgag tatgagtccc
    14941 acagcgattg aacgaccaaa cgatccatta tctgggcatg ggattgcaca atggacggca
    15001 gggcgaacga ctgctttgaa aaattttgcc aaagagcgaa acaaagagtg gtcagattta
    15061 ggtgtacaag ttgagttttt gattcatgaa ctaaacacaa cagaaaaagc ttcacaagtt
    15121 gcattacaag caaaaagcgt agaggaagca acagaacagt ggcaagtgaa atttgagcgt
    15181 gcagggatac caatgatggg gaatcgtatt acttttgcga atcaatggta cgcaaaacta
    15241 ggaaagaatg acagtgatcc cgtcgcttca attacattag aaaatggggc aaacgcagga
    15301 agtaaagact tgaccgatta ttgtctagaa acaacaggaa caagcagttc ttccgatagt
    15361 aatgtattaa acgtagcaaa aagtctgatt ggttattttc attattcaca accgcaacgg
    15421 gcacaatttg gtagtgtcga aaatccagat aaaaatggct atgccgattg ttcgagcttt
    15481 gtgtggcttg ttttaacgaa agcagggtat aaaacgcctg cgaacgttgg gtggtttacc
    15541 ggctcaatga cgagcgacgc aagaggaagt aagcaatact tacaagaaat acctgagagc
    15601 gaggcaaaag ctggcgatat tatcattgtg aacttaggtt ctggtgtggg aaatgatgga
    15661 cacacagctg ttttagcgga agattggcat ggactttcta cgaaagtaat cgaacaaggg
    15721 ggaatgaata ctaacggtgt aggtgaaggg caagtcaatt tatcctttgg gtatttgctt
    15781 aatggtggtg atatttgttt tgctcgaccg ataaagaaat aagaggtgtc aaatgaagaa
    15841 acgatttatt tatgtttgtg taggcggatt gttcttattt ggtgtaggca gtacattttt
    15901 tcttattcat gaaaagctac agctaacagc aaaggaagag aaactgacgg tggagataaa
    15961 caagttccaa gaagagaaga aaggacagga agaccttgta acgtcactca aagaacaact
    16021 tcaacaagta gaaagcgagg tcacgacgtt aaaaaaagca aatgaagaat tgaaaaacaa
    16081 tgattcaaca gaaaaaggaa acattcaaac ctttcaaaaa acagtgacag aagcgttcga
    16141 ggttctgttt aattatgagc ctgattcttt tcaagaacga aaaaacaatt gtgcaccata
    16201 tctttctgac aaactgatga agcaatattt caaagaaggc gtttcctatg gtgattcaaa
    16261 cggggtgtct tctgaattag agaaatgttc tgtttattta aaaagcacac aaaaagcgac
    16321 tatccaaggg ctcgttgtgg tgtcgtatcg caataaaatt ggtgatagtt cctggaataa
    16381 tgccatgaat atttttgacg taacctttga tccagcgtca aagaaaatca cgtttatgca
    16441 aaatttggga agtagcttta atcaacagct tttagaagaa taagaaaagg agaaggttat
    16501 ggagagacaa gaatcaataa gagaggtaca agaagtacca acagaacaag caaaagaaac
    16561 ccaagaagga cgttcttttt cttatggaaa gaatttttct tttttacgcc cgtcgattca
    16621 aatcagtgtg ttcgttttgt ggcggataca caataatcgg tattcggtag gcagtcaact
    16681 attttatcaa gaaatttgtc atgctttatc aaaaaaatac agtcaaaatg cctacaaaga
    16741 agcacttgcc tttttggagg gggcaagtgt ggttgtccac gaagtagtga ttgtggacaa
    16801 aataccgaaa ttattgttag aacggtatgg catttctcat gagtaatgca aaatttaatc
    16861 cacaagcgtt ccaaactaag ccaccgaaac aacagaagga aaagaaacaa aagaaacgtt
    16921 ttttgttcca aaaaaagaaa acagaaccgt cagatcgaat gatccaattt tcgcaaaaaa
    16981 gtgccatgag tgtattttca ggcgcttttt tttgtttgtt tctgttaatg ggtgtcgtgc
    17041 tcctcctaaa ctttggacga gtggatacat taacgcgttt agcagtgaaa aaacaagtca
    17101 atccagaaac attgttaact gaagtcaacc aaaccttgtc ttctacggaa caattgaagt
    17161 acgagggaca acattttatt gaaaggttat ttacttattc gccagaaaaa cgaaacgaat
    17221 gggaggcttc cgtcaatcat tatttagcac aaggattgga agcgtctgat ctaggctttg
    17281 taacgacggc taccaatcga gtagcgaaag atgttcgctt tattaaacta gaagaaatcg
    17341 atttgacaca agcaatgtat cgcctatttt atgaggttac ttttacagaa ggagaacatt
    17401 ggaaaagcag tcaactttct tttccagtca gttatcaaaa caatgtttta aaagtacttg
    17461 atggcgttca atttttgaat ctttctgaga gtaagaatac caattctgtg aactatcagg
    17521 aaaatcgatt ttattcttct gggcaagcgg tcactgaaag cgaagaaatc aagctggaag
    17581 catttgtgaa acggtttttt gagttatatg tcaaaaatga tgaaaaattg gcattgattt
    17641 ctaacgaaaa agggttagaa ggcggcgaat tgatcaatgt acaactggat catgcagtaa
    17701 aaaataaaga aggaaactac tttttgcaag ggaagtatcg tttctatttt gagaaaggaa
    17761 acgagttcca atcaaacttt tcaatggaag taaaaacaag tggcgatagt tattttgtta
    17821 ctacgttaaa tgaataaaat gaacttagag gagaatggaa atgaaacttt ttgatcttaa
    17881 atcgttattt gatgatgtat ctggacaagt catttggcta ttggcaatcg ttgcgattgt
    17941 tctaatggtt ggtgcttatg cgacccaagg ctttgcgcgg gcagcgtttg ccattattgg
    18001 cgtctttatt ttgataatgc tcgtcttatt gttatcgaat gccgttgata ttggtgaatg
    18061 gctaaagaac attatcttta ttaaggatag tgaagagaac gtgggggtaa cgcaaatgat
    18121 tgtgccctgg atacaattat cataagaaaa agacaaactg caaaagcgat cagaagatta
    18181 gaacttctgg tcgcttcttt ttttgtaaaa ggaggtagag tgaacatgga atacaactat
    18241 acaaaggagt ttaagcaccc tcacaaaatt tacgggatta aaggacaaaa aattccctgg
    18301 atttctaatg gcattcgcat ggaacaatta gtcataggtg gggtatttct cttcattctt
    18361 ttgatcctat ttttgattgc gtatgtgaat caaattagtt tcttgcagac actgtttcga
    18421 aagtcctggt tgattatgat ttttgggatt ggtgttctcg tttggacatt gttttcaatg
    18481 aaatgggaca ataaacactt tttcgagtat ttaattgggc gaacgtccta ttatcagaaa
    18541 cggaaacaac gctttgaaca tgaaatgtat gtgccgtttt tccatgaaaa agtgacgtat
    18601 tccctacaca aatggaagga gtgagtcgag taaatggcgt ttttgtttct tttggtcgtc
    18661 tttattatcg ggatttggca gttcaaaaag aaaaaaacga agcttcctcg attagaattt
    18721 ccgatagacg ccattatccg aaattttgtt ttgaccaaaa atgatgatgt gtgggtaggc
    18781 tatcaacttt cttcccaagt atttccttta aatgacattg attttttcaa gaactatctt
    18841 caagatggcg aaggcatact tggacatgat gaatatgact atcattttat caatattcca
    18901 cgtcaattta acctagatac gcacattgaa gaaaccattc gagatttggt aaagggagaa
    18961 tgcgcagatc ttgggaaagt gtactttgaa caggcaggaa agattttaaa agacgaagtg
    19021 caattaaacg agtatcgaac gtatctgttt gtgcgtttca ctacagaaat gaaagtgtct
    19081 gatccgatcg aatattttca attgcttaag catgttttta aaaagtttat ctatcgtatt
    19141 acgggacaac gagttcctat aagtagtgtg ttaccaacgt atgaaacatt agaaaaggag
    19201 ctctatacgg aattgtccaa ttataagccg atagagcgct tatctgccca agagattgga
    19261 caaatttatt attatttttt ccatcgatca gaagaaaaat tgcctgaaag acagcttacg
    19321 ccaatggaaa tttgcgaggg cgtcatttcg aatgaagcag gatacattgc gattgaacag
    19381 ttgaatcaaa cgcattattc gacgttttta agtcaaacgg acgctccttt agccatgtat
    19441 gggagtagct ttattcaaaa tcttcaggat agcttatctt ttccaattga tacgcatatt
    19501 cgacttcgat ttgagcattc aaaatctgat gaacgaaaaa ttaataaaat ggctggacgg
    19561 atttatgaac aagaaaaaga atatgagacc gttgacggca ttttagattc agacgatgta
    19621 ttaaattttg gtacagaacg gttaatggat gtaaaagata agctgaagcg caaagaaaaa
    19681 cgattgtgtt attctaccgt cacgctggtt gtatctgctt cttcaaaaga agaattggaa
    19741 gaacgagtca aagatattga atttgcgatt gagtctacag atttcaaatt ttatcgatcg
    19801 gtagcggatc agctaacgtt gtttaatcaa tgcttaattg gaagtacgca acgttttcgt
    19861 agttacgagc aggtcgctac gacaggtttt gtggcagatt atggcttgga cttaaagaaa
    19921 gaagtaggaa ataagaaagg atttccttta ggacggattg ttattgcaaa aaagtttaaa
    19981 tctgcgaaag aagcgttagc gctatctagt aaactagtat ggtattatcc caatttaaca
    20041 aagaaaagta ttgaaggcgc ggaacatacc aatggaaata cgttgattat tggtccacca
    20101 ggtaaaggaa aatcggtcct tgtaaaatat atttttttat ggctgatgtt tttaggtcaa
    20161 aaaattcttt atattgatcc gaaaaatgaa acccaaaagt tctttttaaa ggcacttgaa
    20221 aaattcggac acataccaga gtttgtggct atgtatgaac gaattaattt tatttcgtta
    20281 tcttcagatg aaagctgtcg agggatgctt gatcctctat tgtttttacc aaaagaagag
    20341 gcaattcaaa ccgctaaaaa tgtgttagga atgttaggag aagtagatac caatcgagaa
    20401 acagcgagcc acaagaaaac ggtgatccaa gacgccatcg atgtagttta caccatgcca
    20461 ggtaagcatt cgttaagtca agttatcgaa gaaattcgaa aaagtgacaa agaactagct
    20521 aaattgattt tagggcatag cgttggttta ggaaagattc tcattgggaa tgagcacagt
    20581 acgccgatac gttttgattc acaaatcaac gtgttaggga cacaaggaat acgcattccg
    20641 acacaaaaag agattgattc gggtcgatta aatagcgaac aacttgcagg aatggcaatt
    20701 atggaagtca ttatgaagtt tacgtatatc ttttctacaa acaaagaaga agacgctgcg
    20761 attatttatg acgaggcaaa agggtatgaa gataccgcac aaggaagcta tttgattgat
    20821 gatagcttgc gaaaaggacg tgcgaataat acggatattt accttgttgt gcaagcgttt
    20881 aaagattacg atcaagaaga taaaaaagag ttaatttctt acaaatttgc gtttcgtccg
    20941 aaacaaaaag aagcacgaga gaaactattg tccttctttg atatggaagt gaattcagcc
    21001 aatttagaaa tgatggaaca attacaaaca ggaacctgtt tattccagga tcatttggga
    21061 agaaatcaac cgattgccat tgatgtgttg tttgatagtt ggttattggc tgtttcttct
    21121 actgaaaatc aagatgaaaa aattcaagca atgttagaaa atgaaaaaca acgataaaac
    21181 aaaaaagagg tggttttttt gcgaaaaaaa tctctgaaat ggtggacgct ccttttatta
    21241 gggggtcttt ttttctgtct acaaggggct actgtttctg ccgatcaaat gaatgatggt
    21301 gcgggtgtca ctgaaaccga tcgttctgaa ggagagaatg ccaacgataa agtagaagta
    21361 aaagaagtaa cgactgatgt ggatcaattg tataaaaatt acaaaaagaa ttcgtttgaa
    21421 ttgatgacca atgactatga gtcaatgaac cttaaaaaaa agctatcgta caatgttgca
    21481 ggagtgatga aaaattttgt ttggagtggc gtaaaaatgt taggagaatt caatgcttca
    21541 atggttgaat ttctctttga attagatatt atttccgcca ttcgagaacc cattcaaaac
    21601 ttaacacaaa aaattgcgga caatatgtta agtattgcag gaactattgg aatcagtttt
    21661 tcggcaatta tgatggcaat caaatatgct ggagaggctc gaatgaaaca agcactccgt
    21721 gtctttctta tgacgatcct tatttttact gggttaagcg tattaaaaga ttctgggaca
    21781 aataagaatc tttttgatgt tcttttttct ttagattcta cagtggaaac ggcgttcgtg
    21841 aaggtcaatc cagtgttaac acctgaagaa gaaacaaatt atgaaacaaa agaaacagaa
    21901 gaggtaacag cggataaaaa agtggaaacg gcaggcaaat tgatggcggc tcgggtcttt
    21961 tatagtaatg tttatgaacc atatctgttg ttaaattatg gcacaaccaa tgtagacaca
    22021 attcgtgaga agaaagtcaa atatgctgga aaagaatatg atcgaatcaa tcttttattg
    22081 gacaatgatt taggtacaaa aataggggag aaaattcatg acaaagtcac agagtatgaa
    22141 gcagatacac tgaaaaacaa gaacatacaa tattacaata gttggaaaaa tacagcattt
    22201 gccttgtttt attttgtcgt gaatctgatt caaaccgttg tttattttgt cttaagtatg
    22261 tttcgattga ttattggaac tatgcaacta ttgttagtac cgatgttgcc gttcttactt
    22321 tttttaggat tgtttcttgt cacaatgaat gtgtttggta actatgccaa agtttttggt
    22381 attttgacgt ttttaaaagg aatgacaggt tttgcatgta tccttttttc ttcttttctt
    22441 agttacgggt tccaattaag tagtacacaa gacgatccat gggaaaaaat tctcttgatc
    22501 attgtttatt tgtttgcgcc gttgggaatt tatttgttcc gaggcttttt aggcagtcta
    22561 tttactggaa gaatgggtgt tcgaggagcg ttagcttttg cgggaaatcc atttggggca
    22621 gaagcaaaat tgagaaggga agccaaacaa cggttgaaag aacgcaaaga agcgagaaaa
    22681 gcacagaaac aaaaagaaaa agaaaacgca cggaagagac aagaacgtca acgaaaacgt
    22741 gggggtggct cagacgttcg tatgccacaa acggcgaaac aagccacacg aagtgctttt
    22801 cggcgtgaat ccgcacaaca acctcgtcat aaagcaccaa atcgtatgga acgtatggca
    22861 caaaaagcaa gagacttgca tgatcaagcg aaacaagcag aagagcaaag tcaagaacgt
    22921 atgcaacgaa cagcaatgga gaaacaggca agaaaaacag ccgatttatt acaacgagaa
    22981 ttgcaaggca atcctagtct aagagaaaga agtagaaaag gtgcgtctcg tagtccgtat
    23041 cgcaatcagg gttctgcaat ggtggtgtca ttagcgaatc caaagcaaaa acacaagtct
    23101 tatggccaga agcatacgcc tagaacagaa ttgaacgatc gaaaacgtcg tcagggacaa
    23161 acaagtcgtc gtcggaagga ggcacttact cccgtaacaa aaacaagttc gatgaaaacc
    23221 cggacagttg caggacaaaa acatgcggta caaaaaccac taagaaaagg gaatgtacca
    23281 cgagcaacag gttctaccac accgaaatct gttcgacgtc cagagggggt tcgggaacat
    23341 atgtcagcag tcaatcgagt aacagcacaa accaaagcgc cttcaaccgt atctaaagca
    23401 tactcaacca tgaaacaaac aaaatcacca aaacaagcac cgaagacacg gagtgagttt
    23461 cgagcaactc aagcaaaaat gaaacgaaaa cgagaaaaac aaccgattta tcgaagagaa
    23521 atgaaacaac gaaaacgata ggaggaaatc gatagtatga tgaatgcacc aaaacgaccg
    23581 acacattttg agcaaatgtt ttctgtcgat cgcaaaagaa aacaagaaaa agaaacgcca
    23641 aaaagtcggt ttcaacaact ttattttatc gtcactttat taggtgtttg tctatatcca
    23701 ttttttattg taggcagtat tcctttagcg attgtcttta tgatagataa aaaagagaca
    23761 cgtgcacata tttatgactt tgagtatgag agcttcttaa aacgaagaag ctctcttttt
    23821 tttatttttg cgttaggttt tacgctatta aatctatttt gttttatttt tgttcttcct
    23881 agagggtatt tgtcgtgtta cttattattt ccattaaact tgttttctca tgcgttgcag
    23941 tttaatggac aaacgatttt tgccctcctt ttaggtggga caggtgcagg aagttgtttg
    24001 attgcgtatg cgtcttttat tgaaaaaaga aaagttgttt caaagaaaga acggcaaaca
    24061 gctttgctaa atagtaaaga atacaaggaa cgtcggaaga ataaatttca ggagtcccaa
    24121 aaatatacag cagagtatca gcaagcgtac caggtagcaa ttgtcataaa agacgttgct
    24181 ttgcggaata aacaactggc ttcattagcc aaagtgattc tattaggaac agatgagtat
    24241 gggctccctt atgtgatgaa ctttagtgaa ttgaaccaac atgtgattat tccagcgact
    24301 acaggttcag gaaaaacgac ggtgatccaa ttactagcag aacatgcgtg taagtttcgt
    24361 atccccgtta tcttagtcga tgggaaagga gcaggagaga ctttacaagc gttaaaagaa
    24421 gtggcagagg catacaatcg cccgattgaa gtctttgtgg acggtggcga tttgcgttat
    24481 aatcctgtaa aaaatggcaa tgatattgcc attcgagata aattaaccaa tctagcagaa
    24541 acagaaagcg tgtattattc gacagcagcc aaagcgttat tacaggtaac gattcagctt
    24601 ttagacgagt tccaaaagag ccataaaatt gaaagaaatc tagtttggtt acaacattat
    24661 ttattgccac gaaacgtctt acatttgttt gcggatcgta ttcagttgaa aaatccagat
    24721 ttatttaaag aaaaagtggt cgtagaaaag aagaaaacca cgaagaaaaa aacgaaaaag
    24781 aatgaagcga tggacaaaac agaacgtgat tttgccgatg tggaacagtt aagtgaagag
    24841 cgaatcgttg aacatgatcg taaaaccgtt gacttagaaa cgtattatct attattaaaa
    24901 gaaaacattt ctctcatgac agaaatcgag caaacgttgt ttaaacggtt gtttgttcgt
    24961 tacgaacata agaagaaccc gttttatttg tatgcgactt ctgagaattt acagaccaat
    25021 atcaatatgt tattggatag tgagcttggt gaactctttg atacttctgg aggaaaaaga
    25081 gaattagata ttcaagaagt tgttaaagaa ggaaagattg tttatatctc gttaaacgga
    25141 ttgatttata gtgagtttat tcggacattg gctcaaatgc ttgtgggaga tattaatttc
    25201 tttgcgtcag aagtgtataa acaagcagga gaaacagcca atcgctcgaa agtcctagta
    25261 atatttgacg aaccagcttc ttatctaaat gataacctta tagacttagt caataaggga
    25321 cgaggcgctg gggtgcatgc ggcgtatgcc cctcaaacgc ttgcggacat tgatagaatt
    25381 gatcctgtcc ttcgaaaaca gttgattggg aatgtgaata cgttctttat tgggaagaca
    25441 aatgacccga cagaaattga ttattgggca gatttaatgg ggacagaaga agacattgaa
    25501 ttaacggaaa tgacgacaca agaagcaggt tattctgata tgggaaaggt tgattggaca
    25561 ggagaaagag gcacgaaacg agacgtagac cgctttaaat tcaatccgaa tcgcattcga
    25621 gcattgagaa caggcgaatt tgtggtgtat cgaacagctg aagacgtcca ggaaccaccg
    25681 cgagtcgtct atattcggaa accaaatttg gagaaacaat atgcgaaata agaaagttat
    25741 tgttaggata cttattgtta gtttcctctt atttggttgt tttaaactga taaaaactaa
    25801 cgaagaatca aatggaaaac taatcaatag taatttagaa gaagtactag aacttcaaaa
    25861 aacgaatcaa tcattttttg tgtatctagc aagaaatgat tgtaaaaaat gccaagaagt
    25921 tgaatctttc ttggaaagat acgacaaaaa attctctagg aatgtatatc gaattgaaac
    25981 aagaaaagaa ccaagacaag aagaacttca aactttttta caacgttacc atgttaaaag
    26041 tgtcccaagt tttctcgtga ttcatgatag aaacgtcaaa aaaattgaga gaaaaacatt
    26101 cttttttgaa aagacattga atcaatttct tcctaatttt tagtgaaaat tgaaagagaa
    26161 tacgttgaaa aaaacaaaaa attgttatga gttcaaattt ttttcgatcg tatttgtttt
    26221 tcttttgaaa caaattcact cattttttga gcaaaaatgt ttcaaaattt gaacaaaaac
    26281 caattgaaaa ttgaagcaaa tttcactcga attgaatcga ttttgagcta aaatcaatag
    26341 aaaaaataaa cgtattttgt ttgaaatccg tttatttttt ttacgtttca gtattagtaa
    26401 ggattatttg agggttattt agagattatt taaaggttat atgtaataag tggggggagg
    26461 gctgtcggcg cttggtatga aaggcattct ccgacttcca ggtttttgac cattttctct
    26521 caacgtggaa aaaccgccac ttatgagaga aaatggtcaa aaacctaagc gggaagtctc
    26581 cgaataacat accaagcgcc gacagccaac agataaagaa tacactacta aaaatagtag
    26641 tagcataaaa tttaaaaggg aaaaaatcaa cccctacccc agtcttgggg tctcaaaact
    26701 aactcccccc taaccccctc tttcagcctc ttttgaaaga gggggagaat caaccccacg
    26761 gccaacgccc cagtgttctt ggggctgtcc agaacagaaa cttgcgagag agtgggggta
    26821 aaacaagtgt agaggaaact tgaagacttg ttcgaacaca aacttacaag agaggggagt
    26881 aaagcagttg cagaggaatc aggaagattt gttcgaacag aaacttgcaa gaaagcatta
    26941 ttaaattgaa ggtgatgaaa tatgaagtat ttattgcatg gctattttat tgattattct
    27001 actcgcaata gtagtacttt agcgattcta tgtttgcttc atgaattgag agtggttact
    27061 agaaaacagc tatacaaact attgaatttt gacagatcaa ttagtaaaca aggagtaacg
    27121 aatgctttag tgagattata tgaaaaacag ttaatcgatc gaaaaaaata tggaacagaa
    27181 atgtattatt atttgacgaa agagggacat caaagcatag gtgggtatta tacgttgccg
    27241 aaagttcctg aatataattt gaatcaccat ttacaaatta acaattattt gattaaagga
    27301 atggcattgg caaaagacca cccacattta aaacggatta tttcagaaag acgtaccgtc
    27361 tatgaaacga aggatttaaa tcctaagaca aaaggaagaa aattttttgt gccagatttt
    27421 atttttcgtt ttcgagacaa agaaaatcat gagatcaatt ggtcctttga aattgagcta
    27481 acgttgaaaa cacgtcgtcg ctaccgtgaa gcgatttttc cgaaatatat tcgtgagttg
    27541 cgaacacgtc ctgaatcaca tttaatttat gtgacaccta gtggcgcaat tcaagaagaa
    27601 cttcatgcgt ttaagcgtat ttttttcaag cgcgaaactg aaaaagatgc gagtcaacaa
    27661 gaattgtttg agcggttgca tattttttca gctttagagt ttgaagaggg attaaaagca
    27721 ttattagaga atgatccagc agtcaattgg cggacagtag aacgagaaaa aatttatccg
    27781 aatggcgctg ttcaaagtcg tttgaattta tttgaagaat caactgaaaa tgaggaaaaa
    27841 ccaaaagtga agtttacgcc tgaagtattg taaaagaggg tgttcgcttc gctctttttt
    27901 tgtgccagtt tgctggcacc gttcagacgc aagcgtctga accggctcgt ttttaggaag
    27961 gactaacttg tttgatttat ccgttgtcaa agaagtggac gttcgagagt ccctaatcgt
    28021 atagcagtca ttcggcgagt ataacctaaa acataacctg ccaattgcca agggtaaaat
    28081 gcacgctgcg cgcccttggc aatcgtcaga acatgttttc gagccggtta tgccgaacga
    28141 ccgctatacg aaaagggcct ctaaacagaa gtccactttg atccttgaca actgaaaccc
    28201 aaaatcaaac aagcaggcct tcgtctaaaa acgagccgaa tgaagacctt gggggttgca
    28261 cccccaaacc cccgtgcccc cgacacacta ccgaaaaggt gggggtgggt ggcgaaaaaa
    28321 tcaaataaaa cattctattc aagaaaggaa gaaacaacca tgacaactaa aaacgagcaa
    28381 ctgaaagaac aggcaagaaa agagttgcaa gaaagaggtt tgattattga ggggagcttt
    28441 gaaggagatt ttgaaaccta tattggttgt tatgcaagac cgataaacaa acctacagcc
    28501 ttagacccaa tgaacgaaca agaagtaaaa gaacaagaaa aacatgcagt gaatggtttt
    28561 ccgcaagatt tcactgaatg gtatgagtgg gaaattaaca acgggaagct tgaaaacttc
    28621 ttataaaagt cagatgacgt gcaaagagaa tgattctctt tgtacggtaa aaatataaag
    28681 aaagaaggaa aaagaaaatg gcaaaaaaca caccttatca agtaggagac attttttata
    28741 gtagttgggg ctacgaacaa acgaacgtga ctttttggca aattgtgaag ttaacagaaa
    28801 aaacagcatg gtttcagcct attcaaaaga aaacggtcga gcattattcg tccatgagtg
    28861 gaaaagttgt tccaatgaaa gacaagttta tagagtatcc tttagcaaaa actaaagaca
    28921 gtattgtaaa aaaacgaatt gatccagaaa cctatcctga tatgttatgt ggttatcgta
    28981 gttgggacac gtttcataga tataaaaatc aagctgttta tgaaagtagt tattattaag
    29041 gggatagttt cccctttcaa aaataataaa tagaagaatg gagagaaaaa atcatgatga
    29101 attcagtgaa tttagtagga cggttaacaa aagaagtgga tttaaaatac acaccaaaag
    29161 gcaatgcgac aggcacattt attttagctg ttaatcgaaa ttatacgaat gcaaacggcg
    29221 agcgtgaagc agattatatc cgctgtgtca tttggcggaa agctgcggaa actttagcaa
    29281 aatatactgg aaaaggaagt ttgattggca ttaatggacg tcttcaaacg aggagctatc
    29341 agaatcagca aggacagacc atgtatgtta cggaagttct agtgacagat ttctatttgt
    29401 tagagagtaa agaaatcaat gagcaacggt caaaatcaat ggcgaataat caaaataatt
    29461 cttcaattcc aggaaatgag attcaaattt cagatagtta tttgcccttt taagtgaaaa
    29521 ataattttgt tctatacctg atttcttcag gtatagaaca aaaaataaat agagatgagg
    29581 cgatttttta tgaaaatcat tgacagttac gatcaacgaa ccatgcaata tttgggctcg
    29641 tttgattata cagatgaaaa tattattaat tatgttgcaa gtttacctcc ttatcaaagc
    29701 gttcgcttgg tagattttgc aagtgatgaa ttggtattaa caacgatggg caacttttta
    29761 gattatgtcg gagacaaaaa atggttggaa aaaattttgt ctaaattgat tgcgaaacaa
    29821 acaggagaag aacccataaa tgcgattcaa ttgttcaatc gatatgaaca agaaaacaat
    29881 gattaaagag gagagaaaaa atgaaaagat taaaacgaag tatttttatt gtgctagcat
    29941 tgattgattt tgttgggaca ggttgtgaag caattgacgt tcaaaaagct cccttatcgg
    30001 aaataaaaca agcaataaca aaacaagatg aagaacaacc gctaaataca caagtagaaa
    30061 tagtgaatca aaataaaccg agtttttctg aagaagattt ttcgttagaa aatggaacca
    30121 ggcaattttt ttcagatttg gatcgtttaa atcgggtagg tgtagcaagt gcacttttgc
    30181 ataaaagcag gatgccaaca gaagaacgag accgattata cattaagcca actggctgga
    30241 aacaaaagaa aatgaagaat ggcgattatc tttataatcg ttgtcatttg attggctatc
    30301 aacgtgcgac aagtttcccc ataagtatgt aatacgaaac gggaggatta atcttaagat
    30361 taatcacaac taacctattt gacagttgga atgaaagcta tcatgtctgc tgaatcaact
    30421 ggtctgatac tcctacatgc aataaagtct actaggaagt tattgtatga agctaggtaa
    30481 aggcggcaga acaatttcta agtgtggaat catatgaagt tggatatgcc gggacgctat
    30541 aaattactca gggtgagact gtttttaatt aaactgacga acttgcgaat gtacgggtct
    30601 ataaccgact tatattcgaa agatataaga ttgcgaatgc aaagaacatt atcgaaaagt
    30661 aagaatgttg gtcatgaaat tcgagatgga agactagcgg tgtatctgtc aagagttggt
    30721 aaaacatgtt tgattgattc agatgagagt gggaatgcga caaaacgttt tacagaagaa
    30781 atagagcaac tagcagaact gaatatacga tatttttaaa tactttagcg gagaataaaa
    30841 aaatcctcta cagaaaacta cggagggcaa catgtgctat tgtttcaaca atacattttt
    30901 aatttttacc ataacaaaaa agtacttgaa aaaaaacaga aatatgttaa attaaaaatg
    30961 aaaaaatgga agcggttact tattcttttg aatctaagat aaaaattcaa aagaataaca
    31021 taacccaaat atgattatac ttgagattct aaatatttat cgaaaaggaa tttgttttaa
    31081 ggaaaaatga ttctatggac ggttattgat ttatatttac caaatggcta ataaaatagt
    31141 atttcttata ttgtatagtt tttgttttta tgtgaaaaca tgagcaatag aattgtacta
    31201 gtaggataag gatggatgga aaatgaaaaa aactattttt gttttagcga tttttatatg
    31261 ttttgggcta cttttggtag gctacttgtt gtttaattct aatgaaatga gaactatttc
    31321 tacgaatgac ttgttaaaaa aaagtgaatt aaaggataaa aaaaaagaat ctcttgcctt
    31381 tataatgttt actcaagaag agtgtgagta ttgtatagca ataaatccaa tcatagatag
    31441 tgtaaataag gaaactttga ataagataaa gaaatatgat atatctgtag attttcaatc
    31501 taaacaaaag tgtaaaatta agtttgttcc gactattgtt gcatatagta agaataaaga
    31561 agtggggaga attgacaatg aattagataa tttgtatcga aaaaagcaag atggttcaat
    31621 tgtaatagac aagaaaaaaa caaagacatt gcttatagat tggatgaaaa aaatggataa
    31681 acaaggagaa tatttgtatg gagaagatta aacagacatt tccgttaatt aataaatatt
    31741 ggaataagaa aaaaattttt atatttttta ttacgtttct gctgtcatct ttattttctc
    31801 taattttccc gtatctattt agtaatgtta ttgatttata tttagagaat gagttatcac
    31861 agaatattgt gcgaatagta acgattctta tatttcagtt atttttagtc ttaattaaag
    31921 aagtgtatgg atatataaaa actatttatg catttgaagt tgatttatag tcaagtccag
    31981 actcctgtgt
//
LOCUS       DACDYI010000028        31891 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.028, whole genome shotgun sequence.
ACCESSION   DACDYI010000028 DACDYI010000000
VERSION     DACDYI010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.028"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..189
                     /locus_tag="IWM52_001266"
     CDS             <1..189
                     /locus_tag="IWM52_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9973525.1"
                     /translation="NSNGILRRNGLPKSIDFREVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
     gene            complement(1055..1585)
                     /locus_tag="IWM52_001267"
     CDS             complement(1055..1585)
                     /locus_tag="IWM52_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HAP9973526.1"
                     /translation="MRLGLIIKNILNLPKTIYFNYKFLHISDAWRLPILVHRKVRITR
                     KSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISIEGILRIQGPAFIAQGAHIDIRKN
                     AVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDTLFMDTDSHTIFELNTHRILNKNN
                     KIVIGKHCWIGAKSVI"
     gene            complement(1582..2199)
                     /locus_tag="IWM52_001268"
     CDS             complement(1582..2199)
                     /locus_tag="IWM52_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP9973527.1"
                     /translation="MIIVIAEIITDILYKLYEPFGFAVLLTILGLVYFMYATDGIAAG
                     EGYKRILMELYLHLKKDRKYRKVCALIFYTAMILFTTLINRTIWLNPLSDVMGDWWIY
                     KYNTTTGTKELSTACLENIILFIPYVYMVMSIKYENIKKPKIWSSIVYGGKIAFYFSA
                     IIEFLQLFLRLGTFQISDFVYNTLGGILGVILYKLILITKRDGER"
     gene            complement(2781..3002)
                     /locus_tag="IWM52_001269"
     CDS             complement(2781..3002)
                     /locus_tag="IWM52_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HAP9973528.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
     gene            complement(3180..4712)
                     /locus_tag="IWM52_001270"
     CDS             complement(3180..4712)
                     /locus_tag="IWM52_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9973529.1"
                     /translation="MIINRKQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAG
                     LNNLFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVF
                     TLGLLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVI
                     NVSMLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVG
                     ELEKETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIM
                     SIFAIIYESCIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFITLFQPFMII
                     WVGSDNLMSISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNI
                     ILVNWFGLYGVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISLCVIAFVA
                     SYLSYFACSYVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILKVLTSVLV
                     KR"
     gene            complement(4848..5933)
                     /locus_tag="IWM52_001271"
     CDS             complement(4848..5933)
                     /locus_tag="IWM52_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9973530.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
     gene            complement(5948..6667)
                     /locus_tag="IWM52_001272"
     CDS             complement(5948..6667)
                     /locus_tag="IWM52_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP9973531.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            complement(6664..7974)
                     /locus_tag="IWM52_001273"
     CDS             complement(6664..7974)
                     /locus_tag="IWM52_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP9973532.1"
                     /translation="MRISIKRWSGADWTLFTLAFFAISIVLVYTFNAPRAILYLGDII
                     NIFVLLNALSFRRRKGIYASKNEMLIVLFLLVSICSSVINLENIGLIIWGIRNNTRMF
                     IVYINCATYLTIKDYYKVFAIIEKLFWISLPLCLIERFMVTYPTGTIVGDMIGGVFWN
                     YSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAALFMSALAELKVFIPELFI
                     IFVAIVFTLKVDFKVVIKILSCVILVSIVLSFFITIFITLNDNGQSYADNFTIQGLLE
                     YASRDSGYDGVGDLNRITGISRVVDEIFNNNFRLVLMGVGIGNAEFTNFFVSEFYKSF
                     GNLHYQYFHHIWLFIEVGILGVSIFLFFIIKTFFNAKRSFMKDKDASLVKVIAIMSVF
                     FFVYNTTLRGETTGPLIYMILAMPEVVKRKEHYYSEKNTKGEIL"
     gene            complement(8001..8591)
                     /locus_tag="IWM52_001274"
     CDS             complement(8001..8591)
                     /locus_tag="IWM52_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HAP9973533.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
     gene            complement(8909..9970)
                     /locus_tag="IWM52_001275"
     CDS             complement(8909..9970)
                     /locus_tag="IWM52_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9973534.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYLQHSKITSFIKD
                     CIYYYRCNNNNSLTHNFRDNEFDCNIELVKKIEGDLSYILENEIFVNKTRRLLLGRCR
                     TCIQKAVRYASFNKEFDLNSYLSKILMNDELIACIHSYPYKQNPIKQRIFNYFLLNQS
                     IIGVKTLAYLSGMGRNRRF"
     gene            complement(10538..11605)
                     /locus_tag="IWM52_001276"
     CDS             complement(10538..11605)
                     /locus_tag="IWM52_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9973535.1"
                     /translation="MQRVLMIGNDSSVKGGITSIITQMLNFNWEKKNIEMTFIPSYKG
                     GNAIFKSLYFMYAFAKIIGISLLSKPNIIHMHMSYRGSFSRKYVLNKWFRLLNIPVII
                     HLHGSEFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENS
                     IVVPEYTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDE
                     EISLKELIEEYNLQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYG
                     MPVIATDVGDISSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAIT
                     KFNDGNFFDRILNLYQKGSKE"
     gene            complement(11625..12104)
                     /locus_tag="IWM52_001277"
     CDS             complement(11625..12104)
                     /locus_tag="IWM52_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta(1,3)galactosyltransferase EpsH"
                     /protein_id="HAP9973536.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
     gene            complement(12121..12582)
                     /locus_tag="IWM52_001278"
     CDS             complement(12121..12582)
                     /locus_tag="IWM52_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9973537.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFFLCTILINLFKSIIIYIKEKPDLVICTGVLAMIPICLITKMM
                     GKKLIYIESFAKVTSPTQTGKLMYHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            complement(12596..13846)
                     /locus_tag="IWM52_001279"
     CDS             complement(12596..13846)
                     /locus_tag="IWM52_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAP9973538.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
     gene            complement(14224..15675)
                     /locus_tag="IWM52_001280"
     CDS             complement(14224..15675)
                     /locus_tag="IWM52_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP9973539.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(15996..16760)
                     /locus_tag="IWM52_001281"
     CDS             complement(15996..16760)
                     /locus_tag="IWM52_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAP9973540.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(16788..17486)
                     /locus_tag="IWM52_001282"
     CDS             complement(16788..17486)
                     /locus_tag="IWM52_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAP9973541.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(17498..18277)
                     /locus_tag="IWM52_001283"
     CDS             complement(17498..18277)
                     /locus_tag="IWM52_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAP9973542.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(18293..19237)
                     /locus_tag="IWM52_001284"
     CDS             complement(18293..19237)
                     /locus_tag="IWM52_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9973543.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(19283..20062)
                     /locus_tag="IWM52_001285"
     CDS             complement(19283..20062)
                     /locus_tag="IWM52_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973544.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
     gene            complement(20442..22517)
                     /locus_tag="IWM52_001286"
     CDS             complement(20442..22517)
                     /locus_tag="IWM52_001286"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAP9973545.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(22519..23436)
                     /gene="glyQ"
                     /locus_tag="IWM52_001287"
     CDS             complement(22519..23436)
                     /gene="glyQ"
                     /locus_tag="IWM52_001287"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAP9973546.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(23822..24640)
                     /gene="recO"
                     /locus_tag="IWM52_001288"
     CDS             complement(23822..24640)
                     /gene="recO"
                     /locus_tag="IWM52_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAP9973547.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKEIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            complement(24783..25682)
                     /locus_tag="IWM52_001289"
     CDS             complement(24783..25682)
                     /locus_tag="IWM52_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAP9973548.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(25697..26098)
                     /locus_tag="IWM52_001290"
     CDS             complement(25697..26098)
                     /locus_tag="IWM52_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAP9973549.1"
                     /translation="MPMDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFTVIVGLILFVPKIYHLLFN"
     gene            complement(26076..26552)
                     /gene="ybeY"
                     /locus_tag="IWM52_001291"
     CDS             complement(26076..26552)
                     /gene="ybeY"
                     /locus_tag="IWM52_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAP9973550.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(26581..28773)
                     /locus_tag="IWM52_001292"
     CDS             complement(26581..28773)
                     /locus_tag="IWM52_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAP9973551.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNTGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(28797..29768)
                     /locus_tag="IWM52_001293"
     CDS             complement(28797..29768)
                     /locus_tag="IWM52_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAP9973552.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            30473..31852
                     /gene="brnQ"
                     /locus_tag="IWM52_001294"
     CDS             30473..31852
                     /gene="brnQ"
                     /locus_tag="IWM52_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP9973553.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
BASE COUNT         9566 a         6490 c         4758 g        11077 t
ORIGIN      
        1 aattctaacg ggattctgcg tcgtaatgga ctgccgaaat caatagattt tagagaagtg
       61 aatcagacat ttatttccag tgtcagcaat caacgcaatc atattccaag aaaatcattg
      121 aattacagaa caccaatcga aatatttttg agctatgtac aagaagcatt ttattctagc
      181 ttaatttgac aaatcatata atttaaaatt aacattgatt ctcctatgaa aatatctttt
      241 acctttctcc tgtgaaaagg aaatttcctt ttttatagtg ttattgatat aatcagaata
      301 ttcatcaaac tctgtatctg gagttaaaaa tctacagtaa catctataag ctttgctagt
      361 aatcttcttg ggttctctct ccattgtcaa gcaacatcaa aacacctact tactattcga
      421 ttcttttctg ccaaaaacga gtcagcatca ctcacaactt atgtgttgct gactccgact
      481 agtcctttgt tcaatatcaa cttattaaac agatataata tttttcttta tttcgtatac
      541 tccaagactt ccatctttct ttcatcttat ctctgaaaaa aacacagcaa agtgccctta
      601 ataaatatac attagaattt gcaaattttt tcatcttaag atatatccta ttcatattct
      661 ttacaatcct aatgggtgat tacaaatcta tttagttcaa gttctacatt ataaacacct
      721 ttcttgatag tatcatctgc tgcacaagcg atgtcgatcc acttattagg ggatttacgt
      781 gctcaaaagt ggctattcct tttgggaagt ccatccataa taatggtcca tggatttttt
      841 tagggtcttg tcgttcaaat ttctcaaaaa ctttattata cttccatagt tttataatta
      901 tttggtttca ctttggctcg ttcaaacacc aacttgtatt tcttttcaac gttcgaactg
      961 gagccccccc tataatctca ttattatttt catgactttt tactaccgtg gaattacaag
     1021 caacaacaat attatctgct aaatgaacac ctttctatat cacgcttttt gctccaatcc
     1081 aacaatgttt acctataact attttattat ttttattcaa aatcctatga gtattcaatt
     1141 caaaaatcgt atgtgaatcg gtatccataa ataatgtatc ccacgatact gtacagccat
     1201 ctttgaattg aatttcctta aagcaaatta tactcatttt tcctgtcgaa ttaaactttg
     1261 acccaatatc taaaactgca tttttcctta tgtcaatatg agccccttgt gcgataaacg
     1321 ctggcccttg tattcttagt attccttcaa tacttataat cgtttcttca tttcccgcag
     1381 tatttaatac tggaaagccc aaataaacta tttttccaga ttgtaatact atctcaattt
     1441 tccctctcga tttgcgtgta atccgtactt tacgatgcac caaaataggt aatctccacg
     1501 catctgaaat atgaagaaat ttataattga agtatatcgt tttaggtaaa tttaatatat
     1561 tctttattat caatccgagt ctcatctttc accatctctt tttgtaatca aaattagttt
     1621 ataaaggatt acacccaata taccgcccaa cgtattatat acaaaatccg aaatttgaaa
     1681 cgttcctagc ctcaaaaaaa gttgtaaaaa ttcaattatt gctgaaaaat aaaatgcaat
     1741 ttttcctcca tatactattg aggaccatat tttgggtttc tttatatttt catatttaat
     1801 agacattacc atataaacat atggaataaa aagaataata ttttctaaac acgctgtaga
     1861 taactctttt gttccagttg ttgtattata cttataaatc caccagtctc ccataacatc
     1921 agataaagga tttaaccaaa ttgttcgatt gattaatgtt gtaaataaaa tcatcgcagt
     1981 atagaatatc aaagcacaaa cttttctata ttttctatcc ttctttaaat gaagataaag
     2041 ttccattagt atacgtttat agccctcacc tgcagcaatg ccatctgttg catacataaa
     2101 atagaccagt cctaaaattg tcaataaaac agcaaaccca aagggttcat ataatttata
     2161 taatatatct gtaataattt ctgcaatcac tattatcata attatttctc caatatcaag
     2221 tatacactta tcgcttccaa ttacacatga tcatattaga gtgtattatc acattctcta
     2281 atattcaatt tatttttcct aaaatattcg aaataatcgt cttgataacg agtaagtatt
     2341 tttatacgat tgataacata gtaacaatat ttttaattat agtaaaaaat catctgcctt
     2401 tttcatccac ctaatcttat ttttcatttt attcaacatc cttaaataaa cttccgttcc
     2461 taagctctat aatatcttct taaaaaataa tattatggtg tcgttttcaa actcgtaatc
     2521 ttttacattg gaaaaataca tacttttctc aatgtagcaa ctcctatctc acagtattca
     2581 aaaattcttc aatattatga aatacttgtg catgttcttt aaataccttg tcagtctgtt
     2641 tataatcccc tactaaattt tgcattgctc ccaactcttc gacaaattgt catctccatc
     2701 tgtaaacagc cctaaaaact catataatgc tttttcatca atatttttcc catattattc
     2761 tcggtatagt ttcacaagtt ttacggattc ttcatattgt ttatcttttt ataatattca
     2821 tctagggaca taattacttt tgctagattt cctgcaacaa cacaatcgtc tgatacgtcc
     2881 ttgtttacta aactatttgc acctataact acattatttc ctatatgtac accctttagt
     2941 attctagact tcattcctat aaaaacatta ttacctattt tgatcctctc cgatgatccc
     3001 aaagcttctc catactactt ttaaaaccga tcaatcataa acatgagtca aaattgtaac
     3061 tccttcatat catctccaat ttcaacaaga gtcttcgttg gaacaaaaac tatacatcgt
     3121 tcgcctattc ggcagcctaa aaattgtagt tactttatat atcattctga tgtctcttct
     3181 catcttttta ccaataccga cgtaagaact ttcaaaatat acttaaactt cttattacgg
     3241 atattaaaca gaagaataac taaaatcgat atgacaaccg atataactaa atttatcact
     3301 aaacttatca ctttgaaatt tccgatatta acatacgaac aagcaaaata acttaaataa
     3361 gaagcaacaa aagctattac acacaaactt atgagcttcc aaacatattt acctgccgat
     3421 tcaaatttaa atatattttg gaaaacatta tataataacc agggtaaatc aaccaacaat
     3481 actgaaacaa tggttgccgc caaaactcca tataatccga accaattaac gaggatgata
     3541 tttaatacaa tgtttatcat tgctgtaatt aatggtctaa acctgtctgc gtaccatatt
     3601 ccagcagcat ctttgtataa cccaattaac tgatcaattt catataagaa aaaatgaact
     3661 atcataaata taacaaatga aatactcata agattatcac ttcccaccca gataatcatg
     3721 aacggctgaa ataatgtaat aaagcatgca caactaattg ccagaaacca acctataatt
     3781 gttgtaatat cacaaaaatc tatataattt ttttcttgac tttctacaat aagactgtta
     3841 cctatacctg ctatacatga ttcataaatt attgcaaata tactcattat ggcagaaata
     3901 ataaagtaat aattctgata aatcgcaagt actgaaagac ctaagaaaga tgaaataata
     3961 attgaatctg aagatcgtaa tattgtaccg cctaccttgt ttgtaactaa tccttttact
     4021 ttttttataa tatcgtcaat agtttctttc tctaactcac caacaggagt ataatcagga
     4081 tacatctttt gagaaacaac agcacacatt aagttaagaa ctatttgtcc caaaatagaa
     4141 gccaataaat acaaatagta attttttaag aaaatcagaa catatgcctg cattacaaat
     4201 tggaatgtaa aagtaattag catagacacg ttaattacat catttctttg atgtgcatat
     4261 attagactct ttctatatga aaataaccaa tacgacaaaa cagtatttat caaatacatt
     4321 aaatacaaaa ctgtcatatt taaatttcca ggaatcgaac ctttaactaa aaatctcaaa
     4381 aaaggaatgc ataacaatcc taatgtaaac actatccatc caataattga aaagtacttt
     4441 ttatatgcgt tcagtaaagc acaaatctct tttctattgt cttctgctat aggtttatac
     4501 atacttacag ttagtgcagc acctattccc aattccgcta aatttaacat ttgaaatata
     4561 gatgtgaaca ggttgttcaa tccagcatac tccattccca tctgataaat cataagtgta
     4621 cgcataaaaa agggaatgat aatctgatat aacttaaaaa ttgttccagt aattatattc
     4681 cttttagcat tttgtttacg attaattatc atatcatatg gctccaaact ttctattttt
     4741 tattgttacc taatactata taatttctaa aatcttgaaa tgtaataggt ttcttctagc
     4801 tctttgatta attacgctaa ttttcatgtt tggacaacca caaaaaacta tacattttca
     4861 atcaaagcaa cccattgttt tgtaatttct ttcaaattaa atttttgtac attattatat
     4921 gcgttcagtg acattctcat tgctaaatca gtattcttag taaacattat catgctctta
     4981 gctaattggt ctatattctt atcttcaacc aacatcccat taaaattatg atgtaccaaa
     5041 tctctcggac cagttttaca atcataagaa ataactggta atccataaga ctgtgcttct
     5101 aatataacca taggaaatcc ttcataaaca gaactaagca cataacaaga tgcagattta
     5161 taatattcct ccacatggtc tgaatgccct gctatagtta ctctttgcag atttaaatct
     5221 tttatctgct ggcgaagatc tttttctaat tcaccttttc ctacaatgat aagatcccaa
     5281 tcatctactt ttttttctac aattttccaa gcacttatca tgtcagcaaa atttttctct
     5341 tccactaatc gccctactgc aagaaatctc tttttatcat aatgatattc gtactcgata
     5401 ttagatatac acggatttgg catagttata acaaaatctt ctgataaact atattcatca
     5461 atatgttttt gacgatcttc ttctgtgaga accacaagcc tctttgcata tttttttatc
     5521 aattttttag ccttgaatct cttcttttct ttcagcattt ccattgtcca gtaattaaaa
     5581 tgttcccaag aaatcaattt gcactttgtt cctttaattg cccttgtagt atacaaagac
     5641 aacaaagtat caatatcaat tactatatca aattcatttt tgcatatata cttgtgcaat
     5701 ttccaaaccg gatataaata agttctatat aattttccct ccgttttagg atttaacata
     5761 taatcaacct taatcgtctt atctaaccca aaataaggct ctccactatt ccaaaagctt
     5821 aaaatagtga catcataacc taaatcagac aagctattgg caataatact ccctactctt
     5881 tcagttcccc cagaacgaga aattacacca ctaatgaaac aaattttctt caaagtactt
     5941 ttctccttta taagtgtaaa aattttttca gtgcaagtaa cctgttaaat tttttttgtc
     6001 caattgttga ttttaatcta taaacaaatt tttgtttagg tgttttccaa gatgcattat
     6061 attgatgaat gcttattgta ttactagttt tataaatttt tccagtatca taatccttgg
     6121 cagcaaaata atccgtagga tagatatgca ggccatttat tatttgttcg ctgttatcgc
     6181 gaaccaatcc aatagattca agataatctg taatataata cggactcggt ttcaagttca
     6241 atgtaccatc attttgataa aaacttaatg aatcgtaaat atcccttaaa cctttaataa
     6301 cagaatgtcc ttcttctgca ccaaaaccaa taccgccatt aacccagatt tccccttcaa
     6361 atcccatata ggcatgattt tcacgaagta tatctaacga cttaattagc tcaacatctg
     6421 tatctaaata aaatccacca tgattataaa taatatcaaa tcttgcataa tctggaacaa
     6481 aaccccattt acgttcttta tatgcatcgg ccatataacg gttcttattt aaatcataat
     6541 ttgattcatt ccattcaata atctcataat cagggcaata ctttttccag ctttctatgc
     6601 actttctatc ttttttcgga agtggatttc ccccgaacca acagtaatgt atcttcttag
     6661 gaatcataat atctctcctt ttgtattttt ttcactataa taatgctctt tccttttcac
     6721 aacttcaggc atggctaaaa tcatataaat cagtggcccc gttgtttcac ccctaagtgt
     6781 tgtgttatac acaaaaaaga ataccgacat aattgcaata actttaacaa gacttgcatc
     6841 tttatccttc ataaaagatc tttttgcatt gaaaaatgtc tttattataa aaaataggaa
     6901 tatcgaaact cctaatattc caacttctat aaataaccaa atatgatgaa aatactgata
     6961 atgaagatta ccaaacgatt tataaaactc actgacaaaa aaattggtaa actctgcatt
     7021 gccaatgcct actcccatta ggactaatct aaaattatta ttaaatattt catctactac
     7081 tctagagata ccagttattc gatttaaatc accaacacca tcatatccag aatctcttga
     7141 cgcatattct aaaagacctt gaattgtgaa attatcagca tagctttgtc cattatcgtt
     7201 caatgtaata aatattgtta taaaaaagga taatactatc gacacaagta ttacgcatga
     7261 taatatcttg atcactactt taaaatcaac ttttaaagtg aatactattg caacaaatat
     7321 tataaataat tcaggaataa aaactttcaa ttcagctaat gcagacataa aaagtgctgc
     7381 acaaacacat aaaataaaat ggatgaaatt tttttctccc ttgaaatatc cgattgtaac
     7441 aacagtaact gtcacacaca aaattaaatt caatggcata ttacaacctg aataattcca
     7501 aaaaactcca cctatcatat ctcctacaat tgttccagtt ggataggtga ccatgaatct
     7561 ttcaattaaa caaagtggca gtgaaatcca gaataatttt tcaattatag cgaatacttt
     7621 atagtaatct ttaattgtta aatatgttgc acaatttata tatactatga acatacgagt
     7681 gttatttcgt atcccccaga taatcaatcc tatattttct aaattaataa ctgagctaca
     7741 aatagaaact agtaaaaaca aaactatcag catctcattt ttgctagcat atattccttt
     7801 tctgcgtcta aagctaagtg catttaataa cacaaagata ttaattatat ctcccaaata
     7861 taaaattgct cttggggcgt taaaagtata tacaagaact atggagattg caaaaaatgc
     7921 caacgtgaac aatgtccaat ctgctcctga ccatcttttt attgaaatac gcatcgttat
     7981 tcaccttctt tcgctaaaaa ttattctaaa aatacttttg ttaaattaac ataccctatt
     8041 atttttgccg gtacacctgc aagaattgca ccattctcta gacaatcttt gataacaata
     8101 gctcctgccg caacaactac attgttagct atttttacat taccaataat ctttgcaccc
     8161 acgcatacac gtacattatt tccaattaca ggacacttag gatcgattcc atcatttcca
     8221 atacagttat tcccatataa gtaacaattt tttcctatta tggcgttacc attaataaca
     8281 attccttgtg tatggtaaaa tcttacacct tcatcaaata cattttcacc acactctata
     8341 ccacattttc ttcccaaact attcattctt ctgcaagaca caatatacat aatcgcataa
     8401 atgatatttt tctttctctt tgaataataa taagatgtaa ttctcattcg acgagtatac
     8461 cgccaagcgt tgtagtccgg atgtgatttc attattttta gtaattttgt gtgttgtcct
     8521 ttagattcta tatatagatt cttttccata cgaagaaccg cttttaaata ttctttactg
     8581 ctaatttcca ttcatatctc ctattaattc aatttatact taacaatttt attcggattt
     8641 cttgcaacaa gagcatcatt caatgctact gaatctacaa cgatggatat gttatcttca
     8701 tcgttgattc caccggatac aatattactt ataaaaatcg taacattttt tccaaaagaa
     8761 gggatgtccg tgatgtataa tgcccatttt ccgatatttc cagttattta aacctaacga
     8821 atttttacaa tagtacaagt atcacaatca ttaatatata actggaatat aaatctccaa
     8881 aaattcacta tagtacagtt ttttatgatt aaaatctcct atttctaccc attccgctta
     8941 aatatgctaa tgttttaacc ccaataatgg attggtttag taaaaaatag ttaaatattc
     9001 gttgtttaat tggattttgt ttatatgggt atgagtgaat acaggcaatc aactcatcgt
     9061 tcataagtat cttagacaaa taggagttta aatcaaattc tttattaaaa ctagcatatc
     9121 taactgcctt ttgaatacaa gtacgacagc gtcccaacaa aagtctcctt gttttgttta
     9181 caaatatctc attttctaat atatagctta aatcgccttc aattttttta actaattcaa
     9241 tattacaatc gaactcatta tctctaaaat tatgtgtcaa tgaattatta ttattacatc
     9301 tataataata gatacaatct ttaataaaac ttgttatttt tgaatgttgc aaatatgaaa
     9361 tatggaatat gatatcttca gatatataat ccctttctga cggaaacttg atattgtgct
     9421 gttgaattat ctcagatgaa tacagcgcat gccaaactga cattggcaat ataaaatcga
     9481 ctttataact cggctcagtg ccaatcattc caagcaacac tttttcaatt atagcgttat
     9541 ttgtaaatat cgtactatca tatcttgctg gctttgaaat aatattatta tcatcataat
     9601 attcataata tccagcataa catgtgtctg cattactcct ttttatacca tttaacattg
     9661 cttcaatata attagctgca acaaaatcat cagaatcaat aaaagcaaca tattttcctt
     9721 ttacatgttc aagtcctgta tttcttgcca atcccaagcc agcattattt ttatgaataa
     9781 tacgaatcct tgaatccttt aatgcatagt cttcacatat tttattagat gaatccttag
     9841 atccatcatc cacaattata atatctaggt tcttatatgt ttgattacac actgattcca
     9901 aacaacttcc tacatacttt tcaacattat atacaggtat gattacacta attagatcac
     9961 tcgaattcat ccttctatcc tttcactcac gttttctcaa ttagtcaatg acggcatcaa
    10021 cgattttctt aaattctctg aaaaaaatag actctgatct tgatgatgtg aatgacatac
    10081 tatcttcttc tagaaaaatt atttccttta aattatttat taaatcaata atatgatcct
    10141 cgtaagtatg ctttcccttg gaataatgaa aatatacttt tgtattacac aatattgcaa
    10201 ttttatttct taatatgatt atttcactca ttcattactg ccacatatac acaacttata
    10261 accaccccct gcaaagttta gggtaagtta tcagataata tcttcctctt catcatccag
    10321 aatacaaaat ctatttattt atagaaatat cattaatgtt tttatataat gtaccgtgct
    10381 tgtactcttg ctctattaca agcactaaaa cactactatt catttgaatt cttacacgga
    10441 taaaaattta catcgtctat ttcaatattt tttctctcta ttttattcac ttaataagtt
    10501 cattacataa tcgaaacatt ttttattttc cttgtcatca ctccttgctc cctttttgat
    10561 acagatttaa aattctatca aagaaatttc catcgttaaa tttcgttatt gccaaatcct
    10621 tcgaacaacg tgaaaatttt acgtatttat ctttatcatt gatttttagt attgcagatg
    10681 tcaacgctga tacgtcacca ggttgaaaca aatatccatt ttctccatca cgtactgctg
    10741 aagaaatatc tcctacatct gtagcaatta caggcattcc ataagaaatc gcctctagaa
    10801 tagatatagg caatccctcg ttataggacg gtaaaatcat cacatttgat cttctatagc
    10861 actcctcctt tttttctccc acaacccaac ctgtaaattc aaaatacgat tgcaaattat
    10921 attcttctat caattctttg agcgatattt cttcatcacc tgctccagca atctcaaaaa
    10981 taaacttttt ttctggatat tgttctgcaa tgtttttaac tgcttctacc aaatcgtgta
    11041 caccttttcg ctttatcaaa actcccataa acagaaatcg gcacattttt gaattccact
    11101 ttatggtata ttctggaact acaatagaat tttctacaac aacagtttta caatttggct
    11161 caatttcttt aattatcttc ttccattttt ctccaagtac aatcgtggaa tcagactctc
    11221 ttaacagcaa gcgaatctga ttttgttttt ttacagaaac actatcatac cattttttaa
    11281 attccgatcc atgaagatgg atgattacag gaatatttaa taatctaaac cacttattca
    11341 gcacatattt cctactaaaa ctccctctgt acgacatgtg catatgaata atattaggtt
    11401 tactcaataa agagatacct ataattttag caaatgcata cataaaatat aaagatttaa
    11461 atattgcatt tccaccctta taactcggta taaaagtcat ctcgatattc tttttttccc
    11521 aattaaaatt gagcatttgt gtaattatac ttgtaattcc accttttacc gagctgtcat
    11581 taccaatcat aagtactctt tgcataacgc tttactcctt atatttatat tttttctatt
    11641 aatacctcta tcgaattaat aatcgtttcc gtgttagata catacttgtt aaacatccga
    11701 actcttgttt ctttaacggc atcttctaaa ttttctaatt cataacaagg atagataata
    11761 ttcatctcat caaattgtct aattaactgc agttggtgat catcgacatg ctcaccatac
    11821 ttagccaatc gaggaacagc aatgaccctt ttaccttttt ttacggcccc aattatagca
    11881 cctgtacctc catgagtaat caccatttct gctttttgca tagtttcagt aaactcatcc
    11941 ctatttagaa atgctttata ggaataattt agtggtttat aatcactata accactctgt
    12001 gcaaatgtac tttcgattat tactcctttt tcaattaaat catcaatagc ttttaacaat
    12061 ctatcaaatt gaaatttttg tgaacctaat gttataaata tcattttttt ctcctattat
    12121 ttaatatatc ccacccaaat aaatagcatt aggataaaat tgtagcatag tttcccactg
    12181 aacataaaat tgatctgcaa aatgatacat caattttcct gtctgagtag ggctggttac
    12241 ttttgcaaac gattcaatat aaatcagttt ttttcccatc atttttgtta ttaagcagat
    12301 agggatcata gctaaaacac ctgtacaaat cacgagatcc ggtttttcct ttatatatat
    12361 aataattgat ttgaataaat taataagtat agtacacaaa aaaaactttt cttttctatt
    12421 tacttgtctc atgtagtacg ttttttcgcc ttttgactca gctgaatatt ctgtctgttc
    12481 tgttataaca aaactctcat acttctccat cagtggtttt aacatcatta actgttcata
    12541 atgcccccca gaggaagccg caaaacatat ctttttgttc atattaacct ctctatcagt
    12601 cacgctgaaa aatatctcta gtgtaaactt tttctttaat atcatccaag caggtatcat
    12661 atcgattggc aataatagaa tcacacatcg ccttaaactg ctctaaatcg ttgataacct
    12721 tacttccgaa aaaagtctct ccatctttca gtgcaggctc ataaatcacc acagtcgcac
    12781 ctttagcttt tacgcgcttc ataactccct ggattgaact ctgacgaaaa ttatcactgt
    12841 tgctcttcat ggtcagccgg tatataccga ttaaaactgg attttccctg ttgacatcat
    12901 actgattata ttcagcgtag ttgtaatagc ccgccatctg aagaacacga tctgcgatga
    12961 aatccttacg ggtacgatta gattcaacga tagcctcaat caaatttcca ggaacatctg
    13021 catagtttgc caacaactgc ttggtatctt ttggcaagca ataacctcca tatccaaagg
    13081 atggattgtt gtagtgagtt ccaatacgag gatcgagaca aacaccattg attatctgct
    13141 gagtattcag ccccttcatt tcagcataag tgtctaattc attgaaatag ctgactcgca
    13201 gtgctaaata ggtatttgca aacagcttta ctgcttcggc ttcagtgaat cccataaaaa
    13261 gagtatccac gttctcctta attgcacttt tctgcagaag tgttgcaaaa acatgagcag
    13321 catttaacaa gcgctcatca gtcatatcag taccaacaat aatacgagat ggatacagat
    13381 tatcatataa agccttgctc tcgcgcagaa actctggact aaaaataatg ttcttactcc
    13441 ccgttgtctc acggactgat tcagtgtagc caacaggaat tgtagacttg atgaccatga
    13501 tggtatcagg attcacgtcc atcacaacct caataactga ctcaacagca gaagtatcga
    13561 aatgctgagt acgactgtca tagttagtcg gtgtagcaat aacaacgaaa tctgcggatt
    13621 tataagcgct ccttgcatcc aaagtagcag ttaagtcaag ttctttttct gtaagatact
    13681 tttcaatata ttcatcctga attggagact tccgtttatt aatcagttca atcttttcag
    13741 ggataatgtc taccgcggtt acctggtgat gctgcgccaa tagtgtggcg atactcaaac
    13801 caacataacc ggtgccggct actgcgatgt tgtacttttt cttcacaata tctcctcttt
    13861 cctctcttaa gtatggagaa tgttactcct acggctattc atcatgtcaa aataacacag
    13921 cttagaatgt tgataaaata attttccaga atgtgataaa atctctttca tttataaacc
    13981 acacatctaa ttttatctat tctccacaag acatgtagag tattctttaa taacaagata
    14041 cgaccttact aaatacttca aaaaattatc atatttaatt cataaatata aatcttcatt
    14101 ttaaatgtat gcccaaaaat ttatctgtaa tgcgtcatca aaatctagat tccaaatctt
    14161 tatcaatttt tatccactca ttctaccact ttagtaatat tttctattta taacactttc
    14221 gtcctacatt gatccccccc tctgaagtac agtttttact gtcttaaata aaattttaaa
    14281 atctaacccc atactccaat tttttatata ttccgtgtca agttttgcta cttcttcaaa
    14341 atcagtaata tcacttctac cacttacctg ccacattcca gtaattcctg gcttaatagc
    14401 aattcttgca cgatgatgat aatcatattc ttcatactct gatttaaggc atggtcgaaa
    14461 tcctataagg ctcatctttc ctgttagaac gttgaaaaat tgagggaatt catctaaaga
    14521 cgtggatcta ataaattgac cgattcctgt cttttttgtt ccatctggaa gtattttatt
    14581 tccgataatt cttggatcaa actccatctt aaacataaga ttattgctca tcttattttc
    14641 tttcattaat tctacttttc tttcttctgc atccatatac atacttctaa acttatacat
    14701 cttgaacggt ttaccattct gacctatacg ttcttgtgca aagaaaattg ggcctggtga
    14761 attaatataa ataagtggag caataaagat aaaaataatt cccgttgcaa tacatccaac
    14821 aatacctgca agaatatcaa ttgttcgttt tataaatgcc tgttgagttg tcataacatt
    14881 gatacttgtt gtaagtactg tatattctcc tatacgacca atagattgtt ttcccaaagc
    14941 actatctgat acttttgcaa gattcaaatg tacagtaagg cccatttcag agcatcgttc
    15001 aataaattct ttcatacacg aagtactttt attcaaaata ataaagactt catctaccca
    15061 tccctgacat aagtaatccg ctgcattttt ggaatcagaa acaactgtca ctccctcaac
    15121 atcttcacca atcatgcttt tatctatgat aatgacacca tttattttat acattttata
    15181 gttatgctct ttcacattac gaataactac ttcagcaata ctactagatg taactataat
    15241 aagtgatttg ttcccctcat ttttcatttt atgtttcaag taatgtttcc ataagaggcg
    15301 agttatatat gtaattacaa cgtataaacc tcccatcaag aaaagaacga ctctagaata
    15361 atcatttcca ccctgaacac taatgagata caaacttcct aacagcacca acataatcgc
    15421 ttgctttaca gaagaagcaa attctatata gtaccctctt tttaatacac ctctatatgc
    15481 ttcaaacatg aaaataacta aaagatctgc cagttcaatg aagactgcca tgttttgata
    15541 cattggaatc acataaggat tccaatctac ctggcgtaag acatacgata gataaaaagc
    15601 aagctggata cacaataaat ctaaaataat aaaatcaata tgtttaaccc atccccttga
    15661 acttttcttg tacattcctt tttactcccc tctttttaat aaaataaatt gtattacttg
    15721 ttttctctcc taaatatctt tctgaaggat tattgactcg acaaattggc ttgtggatat
    15781 tcttatatat agaacatccg taagccaact taaaactcct taacaaaatt tttttactca
    15841 tattgtagat caaaacaagt atccaagaaa tcatatttta tttctttttt ctgcttactc
    15901 aacaacagct aaaagaaaaa tcatcataaa caaattcaac taagccaacg atccccttgt
    15961 gaaatcaagt tttttcttga tttacgaata aagagctaaa ataaaccaaa cttcttcttt
    16021 tttacctctt catagactgg ataactgatt tcatccccgt tgatcagatc ctttgcgact
    16081 tgtttcatct gtactacttt ttccttacca aagtcctttg caatctgctc atagcactct
    16141 tttaagtaaa aggtgcgctt agagacccca tgggcatcag aagcagccat ctgcacgaga
    16201 ttatgtttga ccatcgtttt ggctgttttc tggatgtctt tgccaaatac cccaacataa
    16261 cttggcgcag tgagttgggc caaacatccc atatctagga acggaagcaa atgatttggg
    16321 tcttcgcgaa aatgcacgtt gcgttctgga tggacgatta ctggtgtctt tcctagttct
    16381 aacaactcaa aaaaaagccg ttctgcatat aacggcactt ccagtgtcgg aaattcgatc
    16441 agaagatatg tatcatctaa atctgtaaat aatatttcat ctcggtggat gtcttcgatg
    16501 agcgttccgg taatacgtac ttcttgtcct tccaataacg tcaaaggcaa ctgtcttttc
    16561 tctaattcgg cttgtaacga agcgactaga gaaatgactt gtgatttttc attgctgtat
    16621 tttccattat tgtggtgtgg tgtgcataaa atatgcgtga tgccttgctg aatcgctttt
    16681 tcagccattg caatacttgc ttctaagttt tctgccccgt catcgattcc tggtaggata
    16741 tggcaatgta gatcgatcat ttctcttgtc tcctctcttc ttattcctta atttccgtag
    16801 taataaccag catctttgct atgttctgct ccgttgtaca caacacctaa gactcgtgct
    16861 tgtaccatct ccagcagttc ttttgcttta gtcaaggatt ctttacgtga catattttca
    16921 cgtacgacca ataaagtgcc atccgttttt aaagacatga tctgtgcatc tgttacggct
    16981 actactggtg gcatatcgaa tatcaccacg tcaaataact gtcttgcttc ggataggatc
    17041 tgatccattc ttggcgagct taacaattct gatgggttgg gtggcttagg tccactaggc
    17101 aaaatcgaca agttctcgac tggcgttcgt tggatcacat ctgctacact tcctgatgaa
    17161 ctaagtgctg tacttaatcc actggcattg ttcagctgga atgtcttata gacgacgggt
    17221 ttacgtaaat ctgcatcgac tagtagaact cgttgacctg atttagcaaa aacgactgca
    17281 atgtttgctg cagtggtgga tttcccttct ccaggacctg atgaagtcac gacaatcgtt
    17341 ttgatttgct gaccagctgc ggaggcaaac tggatattcg tccggatcgt acgatattgt
    17401 tcggcgattg gtgaagatgg gtttgttaat gtgatcaaac tgactgcatg agtttgtgct
    17461 gctttttgtt tctgtgttcg tgccattttt cttcctccta gactcttgag cggcttctgc
    17521 gtgatggggc tgttttatcg tttgtagctt ttggtcgtgc aggagctgtt ccttttttac
    17581 ggataccagc gttcaattct ttcgctgaca tttctggaac tgttcctaag attgggaatc
    17641 ctaatgtatc tgtcacatat ttttcatctt tcactgtccg atcgagtaat tccaataaga
    17701 atgccagacc tacacctacc atcattccta acattaagcc agtcattaaa ttcaatttat
    17761 tatttggcga aacaggtgtc gtattggcaa ctgcacctga aatgatcgag attttatcaa
    17821 cattcatcac atcttttgcg ttctcttgga aaacttgtga agttgtgtta gcgatctgtt
    17881 gagcagccac tgcattcgta cttattgcct gaatcgagaa catttgtgaa ttttctgcct
    17941 gattaactga gatggcatct ttgatttgtt cagcagtcat tttcacattg tcttctgttt
    18001 ctaaacgatc tttgacttca ttgagcacta aatcactgac gatcatatct ttatacgtat
    18061 tgatcatttg caggttcgta ttgacatcat tgacatttgc agtgtctgat tgtggcagtg
    18121 tcacgatcaa ttgtgcttgt gaactgtatt ttggtgtcaa gatgaaaaat gttacgattc
    18181 ctgagatggc caagccagca aacattgaga tgatgattat taccaagtgt ttgcgtaatg
    18241 tttcaaaaat ttctttcaag ctgattgtct cttccatttt ttctccactt tcctatttat
    18301 tctctagatt catttgatct tttaattctt ttttcacacg atcaagttct tgatcactga
    18361 ctttttgata ggagatatta tcttgcatga agccttctcc ttgcagctga tcttgcttaa
    18421 tcgtaccaaa tgcctcccga taatctaaag caattttttt catttcatca aagttcatgt
    18481 ctgttttcat attcgactct agagcattca aaacggattg atagttgctg acaccgttta
    18541 gactcaatac ttttttcgct acgccttcta cgatctttct ttggcgccct tgtctaccgt
    18601 aatcaccatt tgggtcttca taacgcatac gagaataaga taatgcctga tccccgttca
    18661 atgtaatttt accgattgga aagtcatagc catcttgtga gaaagtgagg tcattattga
    18721 cttcgatacc accaactgca tcaacgagtt cctttagtcc tttcatattg atagagacat
    18781 agtgatcgat tgggatgtct acatattttt gtacggtatc catcgccatg gcagctccgc
    18841 cgaatgcgta agcgtggttg atcttgtctt ttgtgtcacg acctacgatg tcgacatacg
    18901 tatctcgagg aatgctgacg atcgttgtct gtttgtcttc aggactgact gtggcaacca
    18961 tcatcgtatc tgatcttcct tgatcgaccc gacctaaatc tccagtatca atccccatca
    19021 atagaacaga gaaagattct tgtttgtcca gatcaactgg tttcgttcgt tctccttggg
    19081 ttcgttccac tgattcgtaa gttttctcaa tagaatgtga gagatccaga tataattttg
    19141 ctcctactcc tacaattgct aatactaata cgataagaac tgctaaaata atcaaaaata
    19201 tttttttccc tcttgatttt cttcttcgtc ttgccatagt ttcctccgtt tgctaacctt
    19261 gcttgaatca acgttttaac tgtcaatagc ttatattgct atagcccatt tgctgagcaa
    19321 cagtttgtgc ttttgttgcg tatgcggctc ttattttttc attccggatc tgctctattt
    19381 gactgagcgc cgtcaaatat tgatcgtaag tagcggtagc tgttacttga ccgtctttca
    19441 gcatcgtatc gaatagctga tctagttgcg tgacttgtgc aatttgggta tcagcgaatc
    19501 ctagatattg cataacgact gttttccaag cactatcctt aaaaaagctc atattttcac
    19561 gtacatgggc gacatctgca cttgccaact tttctttgat gatgacatca tccacagctt
    19621 gctgccagtt tgatacgttt ttagtaaata atttattgac agccgtctgg atcttcagtt
    19681 ttgattctgc atcctctaac tgcgttaaga ctgctttctt ttcttcttgg atcgcagcag
    19741 cttctttagg taattctgat tccttgatgt cataatcatc tgccgaaagt ttgatagaag
    19801 atagtttatg tgacaaattt gaaactgttt cttctgttat ttcaggattt aggaaaattt
    19861 tttcttttgt actgtacagc ttatctactt cttttgataa agaggtcacg gtgtcttttt
    19921 gattggtgat ggcagattct gcgtaattga tccgttgatg atattgccgc tgattattga
    19981 gatggtatcc aaacaagcaa atcacaatag cgcctgtgcc taccgccaca gtagccaata
    20041 acttgacgaa cggcttttcc atagattcac tcctaaatat tgatgataga taagtaaaga
    20101 tgtgattcat tcacatcaga atgagcgctt tttattttaa aagcataatt aaactaatat
    20161 attcatagaa atatttataa acaggttttc cctatataat cataccacat acagtttttt
    20221 tgactaggga aattcctaat ttaaaagtaa ataaaacagt aacaagatta taattaatcg
    20281 ttttcatttt tatttcctca cttttcatga gcgttttgaa aattcgaaca aagaataaaa
    20341 taacaaaaat tattaaaata gaattaataa cagctattta taacaaaaaa aacagagaca
    20401 cagcacgttt cgtgcatgct ctctgttttt caaacaaaag actattttgt gatgatttga
    20461 tccaagcttg ctaatgacaa agccatggca gctatttgac ttagttgtgt tagtcggttc
    20521 ttacgtactg ctggttcatc agccatgacc atcgtttgat caaaataatt ttcgatcagt
    20581 ggacgaagag cgacaagctg gtcgtagttt tcgtggatcg tttggtcagc aaaacttgct
    20641 ttcattgctt ctactgcttt gtgtaattct ttctctgcgt cgttttcgaa cagctcaggg
    20701 tctacttctg attgtttttc aagctgcgct tttttcgcta agttcacgac acgtgtcaaa
    20761 gcttccattg aaggtttgaa gtcttcatct gcaagatgtt gtttgaacaa ttgtgctgct
    20821 gcaaatactt ttgtcagatc tttttgttcc gcattcaaaa cagcatcgat gacatcatga
    20881 cgaatattct tcgttgtcaa taattggcgc agacggcctt tgataaagtc gatcacttgt
    20941 tgttgccctt ctgataaacc aatgccgaaa cgatccgtat ctttactgat aacatcgcta
    21001 atcgtttctt gcagaacaga caaaggaaag gcccattttt tcgattcgat gatccgaacg
    21061 acaccgtatg cttggcgacg taaggcataa ggatcgtttg acccgcttgg aatcatccca
    21121 actgcaaaga aagaaaagat gctatctaat ttatctgcaa tcgctaaaac agaacctact
    21181 gtagactgag gtagctctcc ttcactggat ataggcatgt aatgctcacg aatcgctgtc
    21241 gctacagcag gtttctcgcc tttcagaaga gcatattttt cgcccatgat cccttgtagt
    21301 tcaggaaatt cccctaccat gttggttact agatcaaatt tatagatttc agcagctcgt
    21361 tgcaaatcca tcaattcttc ctcagatagt cctacttgtt cgccaatgat acgggcaatg
    21421 acttgtaccc gttgcatttt ctcatagata ctaccgattt tttcatggaa agtaacagtt
    21481 tttagttttt caacgtattg gtcgattgaa gctttcttgt cttcattata aaagaattcc
    21541 gcatcttcta aacgagccgt caatactttt tgattgcctc gaaccacatc ttccaatttt
    21601 acccgatcac cattacgagc agaaataaaa tgaggaagca gcataccttg atcattccgt
    21661 acctcgaaat aacgttgatg ttctttcata gaagtgacta gtacttcttc aggaacagaa
    21721 agatattttt cttcgaagtc gccagaaaag acagttggat attcgactaa attgttgact
    21781 tcttctagta aatcttcgtc tagatcaata acccagtgat ttttttctgc taattcgttt
    21841 gcttgtgtaa cgatcatttc ttgtcttttt tgcggagacg caatgacgaa ttgttcattc
    21901 aatttttctt catattcttt tgcatgcgca aatgtcacat cttcgcctaa gaaacgatga
    21961 cctcgagagg ctctgcctgt tttgacatcc aagatttcga atgggattac ttcgtcatct
    22021 aacaatgcca cgatccagtg aatagggcgg atatattcaa aatcgtattc tgcccaatgc
    22081 atcgttacag ggaaagtcaa acttttgatc acccctgcta agcctgtcaa tacttctttt
    22141 gcttccttac catgagtgta tttcgttaca tagacatatt ccacaccttt taattctcta
    22201 aaagtgatag cgtctgtcgt cagaccttgt ccgcggacaa atccttccgc tgctttcgtc
    22261 cagtttccat ctgcgtccaa agcaattttt ttggctggtc ctttgacttc ttcttcactg
    22321 tccgcttgtc gttctggaat atttgttact ttgattgcta aacggcgcgg tgtggaaaat
    22381 gtttcgatcg tatcataagt caattggttt tcatctaaaa attttgctgt tttttcttct
    22441 aattgtttca tgcttggtgt gacaacatgg gcaggcattt cttccagccc gatttcaagt
    22501 aataagtctt ttgccatctt attcagcctc cttcacggtt tctggatttg ctgtgttttt
    22561 attcaacaaa gggaatccta atttttcgcg ttctgccaca aagattttgg ctacagcacg
    22621 agccatgttg cggatgcgag aaagataacc ggcacgctct gttacggata ctgcaccgcg
    22681 ggcgtctaat aagttgaatg tatggctaca tttcaagata taatcatacg ccggatgaac
    22741 aagattttca tcgatacagc gtttagcttc tttttcaaaa cgatcaaagt tgcttagtag
    22801 caagtcttgg tcgcttactt caaaagaata tttggaatgt tcatattctg gttgtttgaa
    22861 gatctcgcca tatttcacgc catcagccca ttctaaatca tagacacttt caacttcttg
    22921 gatataagaa gcaagacgtt ctaatccata agtgatctca gaagttactg gtttacaagg
    22981 cagtccacca acttgctgga agtacgtgaa ttgtgtgatt tccattccgt caagccatac
    23041 ttcccaaccc acaccggcac agcccatgga tgggttctcc cagttgtctt caacaaaacg
    23101 gatgtcgtgt tctaatggat cgattcctaa aaggcgcaag ctttctaaat aaagctcttg
    23161 aatattctca ggtgaaggtt tcatcaccac ttggaattga tgatgctgat acaaacggtt
    23221 tgggttttca ccataacgtc catcagctgg tcggcgagat ggctctacgt aagcagcatt
    23281 ccatggttct ggtccgattg cccgtaaaaa ggtatatggg ctcatcgtac ctgctccttt
    23341 ttctgtgtca taagcttgca tcagcatgca gccgtttgat gaccaaaatt tttgtaatgt
    23401 taaaatcata tcttggacgg tcaatttttt actcatctgt tttgcccctt tcaattttaa
    23461 agcgttgctg tcaaataaga atacaggaaa gaaaacgaca aaaaatccct atgtctgatt
    23521 acagacatag ggacgaataa ttcgcggttc caccctactt ccgaataaag attcggcagc
    23581 ttcatttatc gtactcgcaa gtgcctcttc aaaaatcgtt tgggtccggc tcccactctc
    23641 ccgaactcgc taaaccacgt gttttttact gatcttggtc aatgtacgta ttcattttgc
    23701 agttttcttg tttctataat agatcctatc actgaacgat ctatttaatc tatggttaat
    23761 aatagacgga actccctgtg tttgtcaaat ggttttgatt ttttgttgta tgttttattt
    23821 actattcttc tttttcaggt gtctcttttt ctttttcaga catgtctttc tctgacgtct
    23881 ttaatacgtg tgcccaagac ttcatttgat tgatgaactt tttgcttttc aaatggatac
    23941 cgacatattc atcatacagc tggtcgatga gttcacggat tgcttgtttg gtttcttctt
    24001 tcagatggat tccactaatt ttatcataag aaatagctga aaacatccga acgaaataaa
    24061 cagctcgagg atctgcatga taacggtgat cgtccatcga ccaatgtctt tcgcatatca
    24121 caccaccata tttggaagaa tagtcaaatt ttccttttgt ttccccgcat acgacgcagt
    24181 gtgtccagat tggtgcaatt ccgaagcgtt gcagtatttg gacttcaaaa atattcgtga
    24241 tgatttctgc atcgtctcct tggtccatcc gttgcagtgc ttgttgcgta aactggaaca
    24301 aatgcgggtc gtactgatga tcttcgattg ccgcatctac tagattcaat aaatatgtgc
    24361 catagccgct caaaaagatg tcttcttgga tgtgtcgaaa cgcatggaca tctttgacac
    24421 tgcttaaaaa tgacaaacct tcacttcgaa aattcccgat ataaactgct tcagtaaaag
    24481 ggagcaaagc cggagctaag ggctgatttt gccgatggat ctctttgata taaaacatca
    24541 ttttgccaga agattctgta aagattttga ctaatttatc tttttctttg taattacggc
    24601 tagataaaat caagcctttt gattctgcaa gctggctcat attctccctc ctaaaaaata
    24661 aatctggaaa agcagattct gccgatccag atcatttatg ttgcgatctt ccgcagcttt
    24721 tctcaacacg cggattttta ttcagtttat ttctatacct aatttacaag aagctcagaa
    24781 tatcagtatt cgtcttttcg gtagccgaag tcttgcagat agactttttt gtctcgccag
    24841 tctttttgaa ctttgaccca aagttctaga aagactttgt ctcctaaaag attttcgata
    24901 tcttttcttg ctttcgttcc tacttgcttc agcatttttc cgcctttgcc gataatgatc
    24961 cctttttggc tatctctttc gacaataatc gtagcctgga tatggatttt atcattctcg
    25021 tttcgcttca tcgaatcaac aacgactgcg acagaatgag gcacttcgtc tcgtgttaaa
    25081 aacaagactt tctcacggat caattcagaa acgatgaagt attcaggatg atcagtcact
    25141 tgatcatctg gaaaatattg tgggccctct ggcatttcac tgactaatac atccatcaaa
    25201 cgttcgacgt tgtttccttc tgtagcagag attggaatca cttgtgcaaa aggcatctgc
    25261 gttgaataat cttcgatgat tcccaacaac tcgtctggat ggattttgtc gattttattg
    25321 ataatcagat aaacaggaga atgcacgttt tttagacgtt caataataaa atcgtctccc
    25381 cgaccgcgtt tttgatcagc actaatcata aataagaccg cgtccacttc tttcaacgca
    25441 ctgtaagctg cttctaccat aaaatctcct aaacgatgct ttggtttatg gattcctggt
    25501 gtatcaataa aaatcaactg agcttctggt gtagtgtata ctccttggat tttatttcta
    25561 gttgtctgtg ccttgtcact catgatggca attttttgac ccacgattcg attcaataat
    25621 gtggatttcc ctacatttgg gcggccgacg attgcgacaa accctgattt atgttctggc
    25681 atatatgttc tcctttttaa ttaaacaaca aatgatatat tttagggaca aataaaatca
    25741 acccaacgat gacggtaaaa caagcggtca ctaaaactgc tccagctgcc atatctttga
    25801 tttttttccc aatcggatga aaatgaaaat ccgtaaacat gtccacgaca ttttcaaaaa
    25861 cagtattgat gatctctact atccatacga gaaaaacagc taaaagcagc cataaccatt
    25921 ctatccgatc aagttggaaa atcacgccca taaacaaagc gaggattccg aacgcaacat
    25981 gtttcctcat gttgcgttct tcctcaaaaa cagttttgac tccttggacg gcaaattcca
    26041 cggacatcag gaaatgtttg tttttctcta cgtttttatc ttttaagtcc ataggcatct
    26101 aagatctctt tctgtaaacc aaacatttct ttctcatcct ctggtgtcat atggtcatac
    26161 ccgttgatat gcaagaatcc atgaaccgct aaaaagccaa gttcccgatc atacgaatgg
    26221 ccgtaagctt ctgcttgctc tgcagcacgt tcagtagaga tcatgatgtc tcccagtaca
    26281 cgaggcaatg gattctctcc atcttcaaaa atcactggga tttcgtcttc gccctcttct
    26341 tccaaggcaa aactgatgac gtctgtcggt gcatcttttc cacggtaatc acgattgatt
    26401 acttggattg ctgcattgtc catgaaagta acagacattt ctgtttcttc tggcaactct
    26461 aaatatgatg ctgcaaattg cagcaagtcg tcgatttctt tcattttctc ttctgagact
    26521 tggtttgttt catccatgaa ggtaatatcc attatacatc gcttctttct attcttcttg
    26581 ctattcttct tgttcttctt ttatcttctc aacttccgat ttcattttag gatatttgat
    26641 ccgagaatgg aaggtactgc ttaaaccgct gatcaatgat ttttctactt tacggatttc
    26701 ttttaacgtc aatccactgt cgtccaattg tccatcggaa atacgctctt caataagatt
    26761 atggacaaaa ctttcgattt tttcgctcgt tggatggtcc attgcccgta ctgccgcttc
    26821 acaactgtca gctatattga cgattcctgc ttcacggctt tgaggttttg gtccaggata
    26881 acggaattgc tcttccgtaa cttccgggtt gcgttccttt gccttaacgt agaaataacg
    26941 catcagcgtg gttccatgat gctgtctaca aatatcgatc accatttgcg gcatattata
    27001 atcttccaat attttggcac catctgtcac atgtccaaag ataatatgtt tactgtcttc
    27061 tggcaataag aaattgtgcg gattttccgc tcctgaaggc aagttctcaa cgaaaaagct
    27121 ggcgtgtttg atcttgccga tatcatgata atagcaagct actcgagtta gtaaagaacg
    27181 tccaccgatt tctgctacag cattagcact tagattggct accatcatgc tatggtgata
    27241 cgtacctggt gcttcttcca gcaactgttt caataaagga tgattgggat tgctgagttc
    27301 atttaatacg atcacactat catcattgac catcaattca atataggggt gcagacctat
    27361 cgtcagcaag aatgaaaaca gtgcacctgt gaatccgcag actagcgtta accaagtcgt
    27421 tgcatcacca aagctcattc cttgatagac gatcaaaatg acatccatca tcacaggaaa
    27481 aataatgacc cacatggcag cagataatcc ttgttctgag atccgtttac gtctgacaat
    27541 cgttgccatg agcccagaga aaacataaga catcaagata atcgttagaa agttcgtgcc
    27601 aatcgaatca taaaaaatga aaagtgcgga tactgcttgg aaaataccag ccatgatccc
    27661 tgctcttcga ttcaagaaaa agcttaaaac aagtggtgca aaagcagcgg gataaaacag
    27721 cgggatataa gtagcttgtt ccgtttggaa taattggaaa aatttcatca gcactatgct
    27781 gactgacata gctgtcacat agaaaagaac gtattttgtt ctttctcctg tattttggaa
    27841 ttgaagtgaa ttataaatca gtacgccgat ttgaagcaaa acagctaaaa ccatggcaat
    27901 tagcggaaaa atcgaagttg tttgattagt cagtcctagt aattccaatt ttttcatagc
    27961 tgaagcatca atttgattac cttcacgaac aatgatttct ccttggaaga tcatcactgg
    28021 ttgtacagct tctcgtgcgg attgtttcaa ttcttctgtc tttttctcat tcaatacgtc
    28081 attcactacg ataccttgat ccactaagta gcgaatcatt tgttgttgtg ctggtgtgac
    28141 attcaaatat tggatttgct cttctgcttc ttgtttaaaa gcttccaatt cactttcacg
    28201 aacttgtttg ctcatctgtt cgtcaatgag cttcagactc tcatccctca cagtatgaat
    28261 ctcagtttca gtcatttcaa atatcgtcga ataaaaattg ttcggtagtt tttgataaaa
    28321 cacaacattt tctgcattca cattttcaaa atttttcttc agcgcggcga tccgctcttc
    28381 tgtagtaggc tttgggatat tttcatcttc ctttgcctta tcaatctttt cgtcataaga
    28441 tttgttgatg gcatcattcg tcttatcgat caattcaaat agttgcttga tccgattgtt
    28501 ctggtcatct gcaagatctt tttggtaagt gtattctgga gtgactgctt ctgctgccag
    28561 ttttcttttt tgttcggttt cttccgtgtt ctcgatcgtt ttgttcgcac ggatactttc
    28621 ttctgctact tggccttctc tgaaatcatt ggccttttgt ttgacactac taaatgtaac
    28681 gccgcagata atgataaaaa acaagaacag caggaaaggc aatgctgcct ttccaagttt
    28741 ttcttgtagt ttctgcagtg ttttattcaa catgtgctta tcctcctaag caaagcttat
    28801 tcttgatgga gatctgcctt ttcataagct ttgatgattt cggcaactac agggtgccga
    28861 acaacgtcac ttgcttcaaa attaacaaaa tcgattttct tgatttgctt taatgttcgt
    28921 tctgcatgaa caagaccact tgttgtccct cgaggcagat caatctgact cgtatctcca
    28981 ttgacaatca tttttgattg ataaccaaga cgtgtcagga acattttcat ctgagcaact
    29041 gtcgtgttct gtgcttcatc caaaataacg aatgcatcat ccaatgtccg cccccgcatg
    29101 taagctaaag gagcaatttc gatcacgcct cgttccatca atcggttcgt atgatctaat
    29161 ccaaaaattt ggtataaagc atcataaaca ggacgcagat aaggatctac tttttctttt
    29221 agatccccgg gaagaaagcc tagattttcc cctgcttcca cagcaggccg cgtcaaaatg
    29281 attttttgca cttcaccttt tttcaaggca gcgattgcta atactactgc aagaaaagtt
    29341 tttcctgtcc cggctggtcc tacgccaaat acgatatcat gtttggctac cgattcgaca
    29401 tatttctttt ggccgctgtt ttttacccgt ataggttttc cattacggtc tttgacgatc
    29461 tcttcctcat acatctcaac aaaataatct aatgtgcctt tttgccccat tttcagtgcc
    29521 gtcacgacat ctggtgtact tacatggatt cctcgtttga tcaattcctg caatgtccga
    29581 atgactgagg cagccaagga aatctgggat tgttcgccaa tgatctgaat gatttctcca
    29641 cgtgtatgga tggttgtttc tgttgtttct tcaatcagtt ttatgtgctt gtcgtgtgca
    29701 cctaaaagca tgctgacatc atcagcagcc gtcaatctaa tctctaaaga actagactct
    29761 tccgtcaaaa atagacatcc cctttttttc gattcattct ttcctatttt accatactca
    29821 gaagaaacta tatagcaaat aagaaaaggt tctaaaagag ttgcttaacc ataagtacca
    29881 agaaacgtta aaaaatagct gcttccattc tctaaaggtc tcgtttgaat aaaaaaagct
    29941 gatcatgtaa gctatgcata agatagagga caagtattcc gtctaactta gcaagtattc
    30001 tctgggtatt tttcttgttt caaaaaaaga aaacaaaata tttgaaatat tcttgcattt
    30061 caaaaaaaac aggtgtatta taacgtcaac aaataaatat acagcaaagc gtcgaggaaa
    30121 acagtaggct attcagtgat ttattcccag tgagtcatgt aagtgagaaa tgacaataaa
    30181 tcgaatagac gaacatcatt tccgagtcaa tgtcgaaaac gtagatcatt gtggctgcct
    30241 ccattatcag gccagcggat aactttatcg aaaagcgtcc gtgaatgaag aagctttttt
    30301 aagcgaaaaa aggtggtacc gttctagaat ctagagccct tttcccaagt ggaaaagggc
    30361 tttttttata ttttaaggtg gtggttttca tggaatctga tattagtggt atttctagtt
    30421 tctgcaacgt ttattttgtt actcaaaatg ataattattg gaggaaaaat ttatggaaga
    30481 aacaaaaact tattcatgga agcaattatt gatggtcagt tcacttatct tcgggatgtt
    30541 cttcgggtca ggtaatctga tctttcctgt ccacttaggt caaatggctg gatcaaactg
    30601 gatcagcgct ggtgtaggat ttgccatctc aggcgctctt ttcccattat tagctatctt
    30661 agctgtagta gtgacaaaaa gtgatggtct gtatgactta gctaaacctg tcggaaaatg
    30721 gtatgctgcc ttgttcttga tccttgttca tttaacgatc ggaccattct ttggtacacc
    30781 gcggacagca gcaactgctt ttgaaatggc tgctaaaccc tttttgcctg aaaaatatga
    30841 ttcattaggt atgtttttat tctctgctct tttcttttta cttgcttatt tccttactgt
    30901 acatccaaca cgtttggtca aatatgtcgg aaagtattta aactccttgt ttcttgcatt
    30961 attagctatc atttttgtcg tttctttatt ccaacccatg gggaatctaa accaatctgc
    31021 tgcagattct tatcagacga acgctttgac aaatggcgtt ttagaaggat acaacacggt
    31081 tgatgcagta gctttacttg ctttaagtgt cacttttgtc catgcagttc gtgatcttgg
    31141 ttttaaagat aaaaaagtca cagaattaac agcaaaagct ggtagcctga gcgttgcgtt
    31201 agaagtcatg atttattttg gtctggtcct tctcggtgcg atgagtttga ataagatgtc
    31261 tttatcagaa aatggcggaa ctgcactttc tcagatcact aatttttatt taggtaactt
    31321 cggtgctagc ttcttagggg taatggtcac attaggtgtc ttcactaccg ctatgggact
    31381 cgttgtttct tttgcacaag atttccataa actgttccct aaagtcagtt atatgacatg
    31441 gctgcggtta acgacttttg tctcttttgt cgttgcgaat gccggattgg ataatatcat
    31501 ccaatggtct ttaccagtat tgatgctttt gtatccattg tcacttgcgt taatcttgct
    31561 ttccttaaca gcaaaattct tccaaaagag tccatttgtc tatcaagtaa cgatgttctt
    31621 ttctgccgtt ccagccgtat tggatatgtt ggcaaattcg cctgcattag tgagtcagca
    31681 aactgtcgta gcaagcttac taggatttta tcatcattat gttccttttg ctgcattagg
    31741 acttggctgg gtcgtcccaa cgttgcttgg ttacgctggc agcttgctct tttattatgt
    31801 atatcgcgta tcaggatata aacaagaagc aagtgagtta ccagaagaat aaccaaatca
    31861 aacacaattt aacataagag atattaaaca g
//
LOCUS       DACDYI010000029        31702 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.029, whole genome shotgun sequence.
ACCESSION   DACDYI010000029 DACDYI010000000
VERSION     DACDYI010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.029"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(718..3867)
                     /locus_tag="IWM52_001295"
     CDS             complement(718..3867)
                     /locus_tag="IWM52_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP9973554.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3880..5079)
                     /locus_tag="IWM52_001296"
     CDS             complement(3880..5079)
                     /locus_tag="IWM52_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP9973555.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLIDLVVRLNKSTNSSRLPKVEF
                     EDIVAGEGRLNKDVSQKFDNRKGIEFLPNDILYGKLRPYLKNWLKATFTGVAIGDFWV
                     FRAKKSDSDFIYSLIQADRYQKAANDTSGTKMPRSDWKKVSDTVFYIPNDLKEQQKIG
                     IFFKKLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGE
                     LGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPI
                     KDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPR
                     YSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKS
                     FLQKMFV"
     gene            complement(5069..6664)
                     /locus_tag="IWM52_001297"
     CDS             complement(5069..6664)
                     /locus_tag="IWM52_001297"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP9973556.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEMWLQGALEVFKHEQ"
     gene            complement(7248..7955)
                     /locus_tag="IWM52_001298"
     CDS             complement(7248..7955)
                     /locus_tag="IWM52_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973557.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENG"
     gene            complement(8020..8856)
                     /locus_tag="IWM52_001299"
     CDS             complement(8020..8856)
                     /locus_tag="IWM52_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9973558.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(8874..10283)
                     /locus_tag="IWM52_001300"
     CDS             complement(8874..10283)
                     /locus_tag="IWM52_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9973559.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(10297..12120)
                     /locus_tag="IWM52_001301"
     CDS             complement(10297..12120)
                     /locus_tag="IWM52_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9973560.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(12963..13289)
                     /locus_tag="IWM52_001302"
     CDS             complement(12963..13289)
                     /locus_tag="IWM52_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973561.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(13335..13697)
                     /locus_tag="IWM52_001303"
     CDS             complement(13335..13697)
                     /locus_tag="IWM52_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAP9973562.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(13734..13949)
                     /locus_tag="IWM52_001304"
     CDS             complement(13734..13949)
                     /locus_tag="IWM52_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9973563.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            14076..14411
                     /locus_tag="IWM52_001305"
     CDS             14076..14411
                     /locus_tag="IWM52_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9973564.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(14487..16484)
                     /locus_tag="IWM52_001306"
     CDS             complement(14487..16484)
                     /locus_tag="IWM52_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973565.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(16582..16857)
                     /locus_tag="IWM52_001307"
     CDS             complement(16582..16857)
                     /locus_tag="IWM52_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9973566.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            17089..17823
                     /locus_tag="IWM52_001308"
     CDS             17089..17823
                     /locus_tag="IWM52_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9973567.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            17862..19004
                     /locus_tag="IWM52_001309"
     CDS             17862..19004
                     /locus_tag="IWM52_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9973568.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(19099..20490)
                     /gene="sufB"
                     /locus_tag="IWM52_001310"
     CDS             complement(19099..20490)
                     /gene="sufB"
                     /locus_tag="IWM52_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAP9973569.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(20495..20965)
                     /locus_tag="IWM52_001311"
     CDS             complement(20495..20965)
                     /locus_tag="IWM52_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAP9973570.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(20952..22187)
                     /locus_tag="IWM52_001312"
     CDS             complement(20952..22187)
                     /locus_tag="IWM52_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP9973571.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(22184..23470)
                     /gene="sufD"
                     /locus_tag="IWM52_001313"
     CDS             complement(22184..23470)
                     /gene="sufD"
                     /locus_tag="IWM52_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAP9973572.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(23483..24256)
                     /gene="sufC"
                     /locus_tag="IWM52_001314"
     CDS             complement(23483..24256)
                     /gene="sufC"
                     /locus_tag="IWM52_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAP9973573.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(24453..25301)
                     /locus_tag="IWM52_001315"
     CDS             complement(24453..25301)
                     /locus_tag="IWM52_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9973574.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(25325..26011)
                     /locus_tag="IWM52_001316"
     CDS             complement(25325..26011)
                     /locus_tag="IWM52_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9973575.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(26004..27038)
                     /locus_tag="IWM52_001317"
     CDS             complement(26004..27038)
                     /locus_tag="IWM52_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9973576.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(27433..27771)
                     /locus_tag="IWM52_001318"
     CDS             complement(27433..27771)
                     /locus_tag="IWM52_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAP9973577.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(27773..28129)
                     /locus_tag="IWM52_001319"
     CDS             complement(27773..28129)
                     /locus_tag="IWM52_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAP9973578.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(28135..29322)
                     /locus_tag="IWM52_001320"
     CDS             complement(28135..29322)
                     /locus_tag="IWM52_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP9973579.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(29513..30358)
                     /locus_tag="IWM52_001321"
     CDS             complement(29513..30358)
                     /locus_tag="IWM52_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9973580.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            30592..31557
                     /locus_tag="IWM52_001322"
     CDS             30592..31557
                     /locus_tag="IWM52_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAP9973581.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT         9184 a         6633 c         5334 g        10551 t
ORIGIN      
        1 ggactaatac aggtagtcct cttttttgtt attttgatat tctaaatcat tttttattgg
       61 attattccct tgttcgatat agacccaaat tgatctatca ataattatca aactgagtca
      121 tatagagaca aaaatttcaa aaatgacctg ttaaatggtc atcgaatatc atttaaaatc
      181 aatacgacgg agtcagacac ggagtcaaaa caagcccgct gagagtaaaa aatatataca
      241 gtcccatagg taaagtgcca caggactgta tattttttta tttgagatta cctgaaatga
      301 ttccatttac tttttatctc attttttcct actagttagt atcaaatact ctcctatttt
      361 ctcaataaac tattttgggt tacatggtat ctgaaacagc taaaaccttg tcattacagc
      421 aaaaaatcac tttatctccg tttcatttca gatacatttc acttaagcgg attccaaaaa
      481 aacaaaagaa aaaggaaccc ctagttagcc tatgcttttt attggttcct ctcaaaacac
      541 ggtcatataa caacggggtg gggtaaatga atgaaaaagg tggaggaaac attattcaat
      601 agctgtctta aatacttcgt tataaaatgt gaacaaataa aagacatcta tccctatttt
      661 ctacacttcc actttactac catctttgaa tcgtacaatt ttcttaactc ttctcgttta
      721 cctcaattca ttttcaagtg gaatcagctc atcatctaac attttacgcc atgctcgctt
      781 catttcttgt gggtatttaa atggcttcgc ttctggatgg ttggctttat atccttcgta
      841 atccttactg ttgataattg ctttcatatt aggaattgga tcttcacctg gcatatattg
      901 cagattagaa gcatgtaatt ctttctcaga aacaaaccac tcattggaaa agacatgaat
      961 caaatcatca ctcgcttgtg taaaatagcg acgtttaatg acaaagatat cttcattact
     1021 atctaaagtc cccacatcaa acgcatttaa aatttgtcgg taaacttctt tgactccttc
     1081 tgttttattc aatttagcca aagctttttc aatttcttct cgtgcatctg aactattcgc
     1141 cgcataagtt cctagtaatt gatcaatata agtagaatca atatcataca acttcgtggt
     1201 attttcacta taaaattcga tcgtggaaaa atcttctccc tctttttctg gttcttcctc
     1261 tattaatgaa cccttcacgg tctcgtacac gccaatttgt tcttccaaaa gttgaatttg
     1321 attgttcaag gttgaagatt tcaccatgtc atcattgtaa tcgtcataag tcactaacga
     1381 ttcataagca cgattcaatt cttggaatgc tttgacgtac tcgatacgga tttcaagtgg
     1441 cgtatgttca tcaatggatt ccatggatga aacctctttt aacttaccat atgcttgctt
     1501 gaatcttttt ttggattcct gataacttgg ataaatcaaa cccgattctt cttttgcttc
     1561 tgaatacagt tttgtcgcat cctcgacatt cttctccatt gtggctggct ttcggaaagt
     1621 aacaattagt ccttttgttt tttctggata cgtacggttc gtacgtgaaa aagcttggat
     1681 caatcctgca tactctagat tacgatccac aaatagggtt tgaatcgtag gtgcgtcaaa
     1741 acctgtcaat agacgatcca ctacgatcac taaatcaatt tgtttcccaa attgtttgaa
     1801 ttccgctctt tttcgtgcca aacgattatt gatgtctccg ttataacgct caatgtctgc
     1861 taatgaccag gaagtatcat aatactgatt atattcctca ataatttttt gcatttcttc
     1921 ttgttgggca cttgaatctc ggctattttc ttctaatgaa taggtaatag cgattcgtgg
     1981 aaaatcagga tcctccatcg ttcgtccttc acgaatgagc tgattgggaa actcttttgt
     2041 taaccaatca ggctctttcg tcatctcttt gatggcccga taatactttt tggccatgtc
     2101 aatggaactc gtggttaaga ttgcagattt cgttggcctg ccatttcgaa aatcaaattt
     2161 tgtataggca ttatctggac ggaacatttt atgaatgact ttttggatat gttcatctgc
     2221 ttcaaaaaca gctgggtcca aataactttc ttttttgaca ggttccattt gatcaatcat
     2281 acgattgatc tgttctgatg aatactctgc atatgtttct acttctttta atttccgata
     2341 gattttattc tctagtgaag tcggctccac ggtgttttca tgctctactt gaaatcctaa
     2401 gacagagcca tcttctagcg cattcttaat ggtataggta tgcaagacct ctccatactg
     2461 atcatgggtt gtacgtgcca attgcccttt ggcttgtttc cgattctcag gaaaaatcgg
     2521 tgtacctgta aagccaaacc aagtggattt cggaaacatt tttttgattt cttccatatt
     2581 ttcagcactt aacgctcgat gacactcatc tactacaaac acgatatgtt ggcccattaa
     2641 tttttggaag cgattggttc cttttttctc ttcttgtttc tttgcatatt tcaacgccgc
     2701 atctagtttt tgacgggttg taatgattac aacatttgca ttggcatctg ccaacagggt
     2761 ttcacttaat tctttggcac ttcccgttcc aacgatcaag ctattcgctt tggcgttccc
     2821 ggaagaaatc ccagtattaa attccgaagc aaattttgta aactctgtgg tggtttgatt
     2881 atctaaatct ttgcggtcca ctaacatgat cgttcgatcg atccccgatt ttcgtgctaa
     2941 aagcttagta gatacaaaac tagtcaacgt ctttcctgaa ccagtcgcat gccacacata
     3001 ccctgattga tgtttatttg cggcaataaa caaggcttga atcgcatgta cttgataagg
     3061 atgtaacacc atgagggttt tattatcttg atcttcactg acgattgtat aatccgcaat
     3121 caaacgatga gcatccggga tatttaacac ttgtttacaa aactcatata agttttctac
     3181 tttttcattg tctttcgttc gccaactaaa taaaaactta gggtgcaagt ctttcggtaa
     3241 ggcatttgca aaataccgag tggtttgttc attggaaacc acaaacaact gtaaggtcga
     3301 aaagatattg ttccgaaaca taccttcttc tgcatatttt ttgatttggt taaatgcctg
     3361 ataaacacca tcttttgcag ttacttgttt cagctcgatt tggacaatcg gtaatccatt
     3421 gattaataag gtgacgtcaa aacgtcgatc ccgtgcttct atgttacttc cacgttttgc
     3481 aatttgatgg accacttcat aagtagagat tcctccacca atgtcttgat tggaataaag
     3541 gataagtgag acagaaccca attgcgaatc ttcccgttca atcgtgatcc gagccatccc
     3601 attttcacct tttaaccatt tcgcagcatc aaatggggtt ttcgttcgca gcagtaattc
     3661 cgttttgatc gtttcaaatt ctttatcggt caaaggaaaa tcattcagtt ctgcttgatt
     3721 atttgagata attttttggc gtaaattgac ccaaaggtct tcttctgatt ttagatccgg
     3781 acgatacgtc cactgattat gcccttctcc aagtacacga atcaactgtt cttcgacttc
     3841 tgcttcctca cggtgactaa ttttcattct tcttccctcc tagacaaaca tcttttgtaa
     3901 gaagcttttt ttcgtttctt ttagtaaatc taacttacgt tgatgaagag cgatcgtatc
     3961 atctagctgt ttgaagaaag taccgatttt ttgttgttca tcctttacag gaaccttaac
     4021 actgatattc tcaatatcag aactgttata agaaggcaat gcccctactt gggagtatct
     4081 tggcaaattt atagtttcaa acaatatttt accaaaatag atatcccaag tattattaaa
     4141 taaataagcc atggtattgt tatctattaa gaaagggctt gtgaccaaac gttttctatt
     4201 aagcattatt gctgcgccaa ctttagcaaa tataatcgct ggcacatctt ttattggctt
     4261 ccatttttta cgctcaattt gctcattaga tacataatga tttgcatttc tcatttcata
     4321 ctcattacca atgttattca tgtcggaaac tttaaaaaat ggagtacctt cagaaccacc
     4381 ttgctcggca tcaggaaatc caattcccga ttgagtttta cctaattctc ccaacttacg
     4441 ctcttcccaa tcttccgtaa aaccaggaaa ccggatttct ggcacttttg ctccgttttc
     4501 agggaacatt ttttgtaaga atcctttttt cgtttccttc agcaaatcta acttacgttg
     4561 atgaagagcg atagtgtcgt cgagtttttt gaagaatatg ccgatttttt gttgttcttt
     4621 taaatcattg ggaatataga atactgtatc tgaaactttt ttccagtctg aacgaggcat
     4681 ttttgttcca gatgtatcat ttgcagcctt ttgatatcta tcagcttgaa taagagaata
     4741 aataaagtcc gagtcgctct tcttagctct aaaaacccag aaatccccaa tcgctacgcc
     4801 tgtaaaagtt gccttgagcc aattttttaa atatggacgt aactttccat atagaatatc
     4861 atttggtaaa aattcaatcc cttttctatt atcaaatttc tggctcacat ctttattcag
     4921 tctaccttct cctgccacga tatcttcaaa ttcaactttt ggaagtctac tggagtttgt
     4981 acttttatta agtctaacaa ctaaatcaat taacttacgc tcttcccaat cttccgtaaa
     5041 tcctgggaaa cggatttctg gtcgtgtatc attgctcatg tttgaacacc tccagcgcac
     5101 cttgtaacca catttcgttt tcaggtgttg tttgcaggga agagatagct tctaaaagtt
     5161 ctttttctag cacttgctgt tcttgtcgta tcttcttgat gtcatttccg acatgaacca
     5221 tatcaattgg ttcttcttct tcgaaagtat ctacatagcg tgggatattt aagttatagt
     5281 cattttcttt gatttcatca aaggttgcca aatgagcgta tttctcgaca tcttttcttt
     5341 cagcgtaggt ttcgaggatt ttttgaatgt tttcttccga tagtttattt tgatttttcc
     5401 ctttaacaaa ttcacgacta gcatcaatga ataatacatc tcgagtttga cgattcttct
     5461 tcaacacaat caccgtcgtt ggaatcgatg tcccaaagaa caagttcgca ggcataccga
     5521 tcacagcata aatactgcca tcttctagta atttttgacg gatgacccct tctgcagcgc
     5581 cacggaataa aactccgtgt ggtaagacga tggccatggt acctgtttcc ttcaaatgat
     5641 agaacccatg aagaagaaac gcaaaatctg ctttggattt tggcgctaat ttcccataac
     5701 gattaaaccg agaatcatct aagaaagtcg tatctgctga ccaattagcc gaataaggtg
     5761 ggttcattac tactgcatca aatgtgtaag gttcatctgt tggccagtcc ttattcaacg
     5821 tatccccatt gcggagattc atttcttcgg cgtcgacacc atgtaaaatc aagttcattt
     5881 ttgctagatt gtatgtcgtt gtatttagtt cttgcccatg atatttaacg ttatctgggt
     5941 gtgttaagta gttacgtacg tttaacatca atgaacctga acccatcgtt ggatcaaaaa
     6001 cactaaagaa tcgtcgttcc ttttgatcga gcgttacgat ttgcgccatc atatcagaaa
     6061 ccatatgagg tgtgtaaaat tctccggctt tcttccctgc ttctgaagca aattgactga
     6121 tcagaaactc atacgcatcc ccgatcacgt ccccatcgtg ttctaaaaca tctacatcat
     6181 tcaatttctt gatgacttcg gtaatcgtca catttcgttg ttgctcatct gttcctagtt
     6241 tttttgattg gagatccaca tcatcaaata acccattaaa ttgattgtag gtagatgcca
     6301 attgcacaaa ggctttgttt agatcgttca attggaacgt tgcttgtttt gcttgatccg
     6361 ctaagacgtt gaaaagatac tctggtgcaa ttgcatatcc caaaatatca acgattgtgg
     6421 caatcagatc ttcttttgat tcttcatctg aaagtaattc acgatacaac attgtttgtt
     6481 tgcttactgt gtcatattcc tctaaggatt catctgctaa aagaaccact tgttccaaca
     6541 atcgatcgga taaatattta tagaaaatca gtcccaataa ataattttta tattctgacg
     6601 catccatttt actacggaga ttatctgccg cactaaataa tttttgattt aattctgcac
     6661 ccaaaaagat acactcctat ctcttattta aagttgctat ataccctctt attttagcat
     6721 accccctttt ataaaaagca aaaaaaaaag aaagaaactt attcttgcta ataaactacg
     6781 cgaaaggagt ctaacgttgt ttgatataac ttagactgat cgttaactta ttttttatat
     6841 ttctacatag ttatctttag aaccatttca gatacatatg aaacaattac atatgatttt
     6901 ttcataaaaa aacagtactg tctagtagta taaaatatac aaatttctta aaaatttttt
     6961 cactaaaatc actacctgaa acagtctaaa acacttatat atcaacgttt aatgaaacta
     7021 tctccgtttc atttcagata caaataaata tagtcgggct ccaaatccga aaaaaaatac
     7081 ccccagtagc ttatcctaag ctactggaga tgtataagta cggtcatata acaacggggt
     7141 agggtaaata aatgaaaaag atggaagaat cattattcaa taactttttt aaatacttcg
     7201 ttgtaatcag ttacctgtcc gttcttaaat accacagaat aatcttttta cccattttca
     7261 agtaaccgaa gtggaatgtc tttaccaact tgctctgtca ctgcattact gatacctgtt
     7321 agattagcta ataaatctga taaagattga gcatctgtag aaatctctgt atcagtattt
     7381 atcgtgattc ctttatcttc atttacaagc ttccaagtag gtggtatagc tgtgtatgtt
     7441 tcgatgaatg aatcaattct ttcatctatc gtcttcccag tatctacttc tgtctcatta
     7501 ctcttagatg aatcgattct ttcatccatc gtctgctccg taactgcttc tgtctcatta
     7561 ttctcaccgg aaactctctt cacacccaaa cttatatatt gtccatcttt agtgtgcata
     7621 gttaaaattc ccacgctttt actaataaat tcactataac cagttctttc aatagagact
     7681 tcaatgtatt tcacactatc atactcaaaa gaaaaataga catgtaaaag cattttgttg
     7741 tttacatcta cataataatc ctttgactct acttgagacc ttcctatacg gaatacagct
     7801 ttactgtctg atatctctat accagcatcc ttattattaa tctgccaatc tccaagtagc
     7861 tcttctggca agctagtagt ttcttgtgaa cactttacca cttcactggt agaggtcgaa
     7921 acattcacct ccgcactgtt agaagaattt tccaaggaat caggagcctt agcacaacca
     7981 acaagtcttt atatattgtg cagccgatat tttcttcatt cagtacaccc ttgaaatatg
     8041 cagtgttaga taaagcagtt cgtcctgatt tgctgaggca cccatctctt cttgaacgta
     8101 ctccttgatt ttcaaggccg cccgatactc tttcggaaaa cgctgcttca ctgaagccag
     8161 caaaaaatca tccttctccg gcaactcttc cttcttaatc attcgggcaa taaaaaactg
     8221 taaatgcgtc acaaatctta agtaaagcat cgaatcttta tcaacctttt tttcgggaaa
     8281 cagatatcct agcatagcca agatatcctt tagtgctctt accgagagca tggtttcttc
     8341 catatttgct tgttcacttt gggcatttac aatgtgaaac gcaatgtttc cagcctcctc
     8401 ttctggcaac tgaacattca tttttttgtt gatcaattgg atagcatagc agccgatctc
     8461 aaattctttt gggtaatact tttgaatttc aatcagcagg cgattttgaa aaacaatgcc
     8521 tttttgactg cgttcaatgg catgcttcaa gtgatcaacc aaggtaaaaa atagactttt
     8581 attcagctta aagtcaaact tggcgttggc ataatcgata attcgcttga cgcaaataat
     8641 tagatcatcc ggagcagcct caaacagttt aatgtaatcc ttttttagag catcagaaag
     8701 tgaatctaat ttaaatactt tttggatttt gtcagccgga accaaatcgc ctttctttag
     8761 ttgaaatcca atccctttcc ccatcaaaat tacttctttg ccttgcttat ctaagcaaag
     8821 aattaaactg ttgtttaacg ttttaattac tctcattaga ttcaccgttc tctctaagct
     8881 aagccatttc ttgcgattac tttttgatac caaaagaagc tttctttttt tattcgtttt
     8941 aattctttaa gattctcatc ggttcggtca acatatataa aaccataacg cttattgaag
     9001 ccttgatggg tactaacaac atcaatcgct gaccaaggta aataaccaaa aacatctaca
     9061 ccatctgtta aagctgcttg aatagccaga atatgtttgg ctagataatc aattcgatcc
     9121 tggtcattaa ttcgtctatc ttcctcaact atgtcgggtg ccccatagcc attttcagta
     9181 attaaaatag gtaaatcata gcgatcgtac aatttcctca atgttaatcg tagtccagta
     9241 gggtcaacta cccagccata tggcgtttta tctacataag gattttcagc tgcccggtag
     9301 acaccttctt ctcccaacat gatctgttga tcccccgctc tagcggaaac atctgatccg
     9361 tcatttttgc tggcagcgat ggtggcagtt gaataataat taatcgcaat aaaatcgggt
     9421 ttaccggagg ataaaattgc ctgatcctct gcctcaattt taggatataa tccacgatca
     9481 ttcaaatagt tttcaaacaa ataattgtat tttccccgaa ctgctacatc taagaaagac
     9541 cagcctctca atgtttccca attatgggct gcaattgcat cttctggact ggaggtagct
     9601 tgatacatag ccgttaaatt gattgctgga ccgatttttc ctttaagatt caactgatga
     9661 tacaagtgaa ttactctagc ttgagctaat aaaacatggt gattaatttg aaagagttct
     9721 ttttttgaag gcagctctcc acttggtggc aaaccaatcg ctccaggatg aaggatcatc
     9781 gtgttttgct cattaattgt cagccaatag ttcaccttgt caccatactc agtaagcaat
     9841 acctgtacat agtccacaaa ataatcgata gttgctcgat ttaaccaacc gccctgctcc
     9901 tctagaaaat aaggtaaatc gaaatggtac atggtgacaa tcggttcgat tccctgattc
     9961 aggcattcat caattacttg atgataaaaa gaaattccct tttgattggt tttattattt
    10021 tcaatatcca taattcgcgt ccaagcgatg gaaaagcgaa aagcttttaa gcctaattct
    10081 ttaaacagct gtatatcttc tttatagtga tggtaaaaat ccgaggcaac ttcaaaatta
    10141 gctacccctt ctgtatgctg atacttgtca ataatagacg gttgtttccc atcctcctga
    10201 taggcaccct ctgcctggta ggcactaatg gaggcacccc ataaaaactt ctctggaaat
    10261 gctgaaagct gtttgtattg cacaaaaatc ctctccttaa ctaatttgca tcaatggttc
    10321 gtgttccttt aagcttccgc ttgcttcact aattcctgcg taattttcac tgttggtcac
    10381 taccacaatc actgtatcat cgtagccagc gtttctaatg gcagcgaaat cggcgttgat
    10441 taataattgt cctgctgaca cctgatctcc tgcttttaca aatacttcaa agggttcccc
    10501 ttttaaattc acggtgtcga tccccacatg gatcaataat tcaatacctt ctttagaacg
    10561 tagaccaatc gcatgtttag attctaaaat agtaatgact tctccctcaa caggtgaata
    10621 aatgctgttt tcttctggat gaatggctac gccctttccc aatagtccct tagaaaagac
    10681 agcatcgttc acttcagaaa taggacacac ggttcctttt accggatttt caacaatgac
    10741 tgcttcggct attgccgttt gctctgattc atcagaagta tcgtcaaatc caattagata
    10801 agcaatgata aaggttgcaa caaaggcgat tacacacgta ataattgcgt gaacaatatt
    10861 cattggattt tctccaataa aggctggtaa ggctgctaag ccaggggaaa caaaggcata
    10921 tctgactaaa ccgctcaagc cagcatacaa accagcaatt gcaccaccaa tcatggaagc
    10981 aactaatggt tttttcagct tcaaggttac tccatagagt gaaggctcag taattcccat
    11041 taaagcagtg atcccagtgg acacagctag ctgcttcaac tctttgtttt tagttttcgc
    11101 ggctacagca aaggtagcac ctgattgagc aatattagaa gccagcattc ctggcccatt
    11161 gatcatttca gaacccgtac tgctcaattg catagtagcg attggcgtca ttgcccaagc
    11221 tgtacccgta accaccaaaa atggctgcaa accacccatc aaaaatggaa ttaaccaagc
    11281 tgctttgccg ttaatccatt cagcccccat tgctataaga tcgttcagca acgttccaaa
    11341 cggtccaatg acaaccaacg ctaaaggccc cataattaaa aataccagca tcggcttagt
    11401 aaagaattta ataaacgaag gcgaaattcg atccgcaaag ggctctacat aggacatgat
    11461 ccaaacagta aagataatcg gtaaaactga tcccgcataa tctgcagcta ataccggaat
    11521 tcctagaaaa gaaacggcat cgccagtaga aaaaagctgt gaaatatttg gatgaagcaa
    11581 aatgccagca acagtaatcg ccaaaattgg gttgacatca aactttatgg ctgccccata
    11641 ggctaatagt actggcataa aataaaaggc tgcatcagca atagtattca aaatgatgta
    11701 agtttgacta ctgtctgata gtacaccagt caaagtcaag acggccaaaa tggcttttac
    11761 catcccggca cctgttatag ctggaatcat cggtgtaaag gtggtagaaa tgacactaat
    11821 caatcttgaa ataatacttt cttgattgtc tgaagagggg ccctttttaa ttgaagttcc
    11881 cacttccttt tgaatttcct tgaaggctgt ttggacttca ttgccaatca ccacttggta
    11941 ttgtccaccg ctatccacca cactaattac accaagtgtt ttttctaagg ctttcgtatc
    12001 ggctttcctt ttatctttga gttcaaaacg cagtctggtg gcacaatggg ttaagctgac
    12061 cacattttct tttcccccaa cctgttgcaa tacatttctt cctacttctc tgtaatccat
    12121 tattctctct ccttaaagca gtaaaaaaga ccaagtcaaa ccacgcccca atcaacattg
    12181 aagtgttagt ttgtcttggt cttgcctgca taatcagtaa catgccaata acgttatttt
    12241 gctgtaatcc aataatagca cattatgata gcgcaatcaa cagggaattt aattttttat
    12301 cttttcgttt gaccgtatag ggtaatataa cagttagatc acatcaacta gggtatatct
    12361 gcgtatactt actgctaaaa taagtatatc taatattaac tataccagta aaaccattcc
    12421 tacataacca gcgggaaatt cccaaaatcg tgaaagcatc aaaaatgcat ataacggatc
    12481 acacaacttc aacaattctg ctcttgggaa aaattgatga atttggtgag taaaggatgg
    12541 aataaacagc attccaacaa tataggcaac aatagtgata cttgtttttt tggtaaaaat
    12601 cagtaagcaa tagccaattt caatcattcc gagatataaa ggtatcaata gaatgatttg
    12661 ggctagtatt cttaaaaatt aactagcaaa tgtttaaact gatttatttc taatctacat
    12721 gtagtaagta tttcaattcc accaattatc aataaaaata ccaaacatat ccataataat
    12781 tttttctttt tcatttcttt tatattgtta taacagtgaa taacataaaa attcccgaca
    12841 atcattgtaa tatttttttg tcacctgtat tagtatttaa tttataggaa actataaaaa
    12901 ctccctagtg aatttacacc tttgggagtt cctgtttgga cggtatgcat aatacattta
    12961 aatcaattta acattaacac ctcagcatat ggtatctcca catatatacg gtctttcttt
    13021 ggatagtact caatatttat gttgatagca cttccattgt ctaatatagc tttagcccgg
    13081 tatgtgttat ctccattttc atgagtaatc gtcacatcat cacatgatcg atttaagttg
    13141 tactgttctt gtaaaatctg tgttaccaat ggttttgatg acttctctaa tccagatgta
    13201 cctgtcattt ttgaaaaaat aatacctata atagccaaga caacaatacc tataatgaca
    13261 aacttcttgt tcatatttac tttattcaac tttttatctc ctatctccaa aataatttgt
    13321 tcttatccct cttatcagta cattgacatc cctaaaagta caccaaagat tgctccagct
    13381 acccctgcta tcatcaaaat cgtacccatt ttctcatctt tttctcgata taagtagccc
    13441 ataataacag caacagcacc aaatacaaca ggaataaatg ctaacgatac taccatagaa
    13501 atccaaccaa taatcaaata cagcttccct gtctcttgtc catgtacgat ttgttccttc
    13561 atttcattaa ttgtctgctc agtacttttc tttactgtgt ctgttccggt tgtctcatct
    13621 gtttcttgta gtgctccaca aaactggcaa tacttacttt catctgttat ttctttgcca
    13681 cattttctac aatacatgtc ttctcctcct aaaatcattc aattgcttat tagttaactg
    13741 tttactttat taaatacatc agaaaacagc tcattcattg gtacacttaa tatctcagaa
    13801 agcatcttca tttgataggg tttaggaatc gttctccctg tttgccacga acttacacat
    13861 tgctgtgtaa cattcatttt ttttgctaac tccgtttgag aaagtccttt ttcttttctg
    13921 tatcggctga gattacttac ttttttcatg attctcccct cctgttttat tatatacaac
    13981 aatatgatgt atttcaactc ataaaatata aaaaacttca aataccatta caaatacaac
    14041 aacttgttgt actatactga gatgggggtg tattaatgaa acagaaacaa tatgcaaagg
    14101 tcattagaga aaagcgaaaa gctctcgggt ggacacaaaa agaattagct gaaaaaatct
    14161 tctctacaca acaggcagta gcaagatggg aaaattctgt aacagagcct aacctcgata
    14221 gcttaacagc tctttctaga gccctaggaa cccctgtaag ccattttcta gacaatgttg
    14281 tcgtagatta tgaggaagag tttttggctc tctatcgttc tcttagcact gaagatgcag
    14341 ttcgaacaat tgactatatg aagctattaa aaaggcaaga aaatgaacgc aatcagctat
    14401 tgaaagattg aaaaataaat aacccaccga actttaacgg tgggtgtatt gagatgaaat
    14461 aagattaaga gctatggata acctgtctag tttaatacaa ttgggtcttt tctcttcctt
    14521 actctccgtc catcatctct tgttacactt gtaaattgat acagaggaac tatcccagct
    14581 cctgataata tcaattcttt tattgtttca tctatccctg cccctttcgc taaatatatt
    14641 aaaaaactac tacttgcttt cacattgtat agtttcttaa tatccttaga agctcgttca
    14701 agagtgaatc cttgtttaac aaagtagcct ctgtttttta ttaaagcatt tttcacgctg
    14761 ttatccccaa cattacgtag gcgttcacaa acagtgttaa taagttcttt ctctgtcatt
    14821 agatatgtcc cttgccacga taagccctgt aaaacttcat gtgcttcatc taaaatcaat
    14881 tgtttttcct tgctgagttc tgatcgggaa ttttgaggaa atactctttt agcctctttt
    14941 tcgttaaaac atatcttaac ccattcataa gaaaaatatt gatatgtaaa atgaattttt
    15001 tccagaagtg gaaggatatc ttcattcaat ttttgaatca cagaagttgc attaaaaacg
    15061 tctaatcgat agacattgct tctcttatga ttaaagtttc ctaacgcttt ctcttccttt
    15121 aacctattta ggtattcgag catcttcaaa ggaacatctt catcatctaa tttttttatc
    15181 aatcctataa aagtcataag tgatagtaac ttattacatt tatctatgtc ataagtttta
    15241 ccatagtaag aactcatttt tttagctaat tgttcccccg acaaaataca agtcacactt
    15301 tccttactat catcaaaaaa attaacatca gaaagcaaga catcatagac acgtcgcatt
    15361 tcctctagat aattccttat tgtcctgaaa agtttgtctt tcattgattc aagagtgtca
    15421 aaaagtactt ttgattcctc tttcaatttt cgaaaatgtg taggatacaa gtctaggttc
    15481 tctaatagcc aattaaatgt ttctacccga ctgtctttct ttaaaattag catcacaact
    15541 ccaataaaat tttcactgat atcacaatta ctggaatgac aataaaaaat ggaatgccca
    15601 gttttactgg taaaaatact agcagaggga ttatgatcgt tgtgaaacag acatcttaag
    15661 tttttacttg acttaagtaa tttattcata tcaatggtta acaatctttc ataaatctca
    15721 ttaaaagaca tgtcggagga atcaaacagg cagtctttaa accactgttt catttcttct
    15781 ttgtcgtcat tttttatcaa cttaacaaaa tcggtttcat tgactagttc atactctctc
    15841 cctgaatcac tttgacaatt tttagaacat gtcatggtaa aatcaatcgg aagctcagta
    15901 atatctaaca tgttgtcttc attaattatt atcactgcat ctgacttata tcctccaaaa
    15961 aacattctat tcgaagcatt tacattgtca tcacacccgg ggatgttctt tttcaagtat
    16021 tcgtaaacag caaccatttt accatagtca gtaattgctt cttttaatag aaagacaatc
    16081 ttgtattttt ctagattttc agagtaccga atactttttt gtatcgcaaa cgcagaatta
    16141 tgaactaaat tgatagataa aaccttttct atgcttaagt atctactatc atctttactc
    16201 aaatctacta ccttcccctt ctcatcttta acagtgttat cgacatccag aataattact
    16261 ctggtttcca caaaactctc cttgtcattg cctttattct ttgtcttgct tggaataaat
    16321 gattgtgatg aatttagtat ctctacaaac tcatctcttg taacttctct atagttatct
    16381 aaacacctat ctcttacatc agttttttct ttttttgtaa ctggcttctc taataatttt
    16441 tcttgatcta cataaattgg aatattaatc attgatgttt ccatttgtgt ttcctcctta
    16501 taaaattctg ttaaagacta aaacatacag ataataaaaa caaactcttt attactatat
    16561 gttttagtct tatttgttta ctcaaccttt ataccgttcc atgaaactat caatatcaga
    16621 ttttttaaat ctaatgaccg tatcaatttt tataacaggt aacccgtatt gagaaacata
    16681 ttttctaatc gttcctactg atacacctag atattcactt gcctgctgat agtccatata
    16741 ctctagagtg actccagaaa taatgtcttc tagcataggc tttagcactg taattatctc
    16801 tttcacttgt tgttcaactt gtatagaaac aatgctatca atgatttgta gagacatatt
    16861 tccccctcct ttcgtgattg cttgaacaaa ttttctaaga attatcttcg tattttttgc
    16921 tcaaaataaa agtattttgt tattaaccga ttaatcaaac aaaaaatgat taccagttca
    16981 tcgacataca cataatacct cgataaaaaa ggggtgtcaa cagctttgta aaatttattt
    17041 acaacacccc ttttttgagg tatactaatt ttaggaggtg tggtcaatat gataaaacgt
    17101 ccaccaataa attatttaga acgaaaaaag atactcggaa caaaaataaa agctataaga
    17161 aagagtaaaa aattaacaca accagcattt ggtcttatga ttaacaacgg tcaattaatt
    17221 gacaaaaaaa ctatctacga atgggagaag ggaacctatt taccaatacc agagcgatta
    17281 tcccgaattg ctgatttggg aaatatgagt atagaggagt tagtttgtgg caatgtagaa
    17341 gaatatattt tgggaattat tctttataga gatagcattg tactagatgg tataacattt
    17401 ccagataaaa acctgttcca gcatcttcgt caacagttcc caccagtaca ctctaattta
    17461 gatacatggc tggatagata ttccaaatta gaaccagaga tgcaagaatt tattgctaac
    17521 aagacttgta ataaagttaa aaatgaaaaa attagtcttt tcaatatctt aaaaatagaa
    17581 gaattattca tcaatgctat tgtagaagaa tttgacaata acattctttt cctcactagc
    17641 agtatagaag aactactaga aagaatggta gatgaatggc tacctattca attgaaagac
    17701 atgtcttatc ccgaagaagc tgtgagagaa attactgata atataaacaa gctagaacaa
    17761 acgatttcat caattgggaa aaaatacact aagaaaaaaa tgaagggagg tgataccatc
    17821 tagtggtaag tactccttgt aaactaagag gagatgattt aatggcaaaa atatcaaagt
    17881 acaaaaagaa aaacggtcaa gtggcgtgga tgtataaagg acacattgcc accgatccac
    17941 gaactggtga aaaaataaat actacgagaa gaggttttag tagcaaacaa gaagctcaaa
    18001 aagattatga cgagtacaga catcaaatat tatacggtgt taaaaagaaa gcacctgata
    18061 tgacatttga agacctgtat aatgaatgga taactcatca gcgtaccagt gttaaagctt
    18121 caacaatcgc aatctcagta agatacgcaa ataatcaaat acttccagca tttggtaagc
    18181 ttaaactatc gaatatctct gtcccatatt gtcaaaaagt ggtagatgaa tggcacagta
    18241 aatatgaaag ttacgattat atgagaaaac agactgcaca aattttacgc tacggggttg
    18301 caatgcaata tattgacaat aatcctatgg aaaaaacact tcttccacga aaaaaagagt
    18361 atgagaaaaa tcgtaaattt tactcaaaag aggagcttaa taatttactg gatgctttta
    18421 aagattttgg taacatgaaa caatacgcct tcttcagatt attggcatac acaggtatga
    18481 gaaagagtga agtactcgct ttacagtgga aagacataga cacgtttaat aaagaactac
    18541 atgtcaataa aactcttgct gtagatgaat ttggtaaagt tataattcaa tctccgaaaa
    18601 caagagcttc tagacgagtt atctctttag atactgagac tctttctatc ctcaacaact
    18661 ggaaattaca gcaaaaagaa gagtatttaa aacttggata caatactagc tctaaagagc
    18721 aacatgtttt tacaacagtt aaaaacactc tatacattcc aaacactgtt aatgattggt
    18781 taagatatat tttgaaaaaa tataacttgc ctcgtattac acctcatggg tttagacata
    18841 cacatgctag tttattatta gaagcaggag aatctgtcaa ggtagttcaa caacgattag
    18901 ggcatgaaaa tagtaaagtg acattagaca tctatgcaca tattacaaat aatgcaccta
    18961 agaaaacagg tcaagacttt gctgatatga tggctcatca gtaaaagagc tggtcaaaat
    19021 ctggtcattt ctagtataca ggcaaaacaa aaaagctaga acccttatat atcaagggtt
    19081 ctagctccta taacaggttt acccaacact gccttccatc tcataactga tcaatcgatt
    19141 gagttcgact gcatattcca ttggaagttc tttggtgaat ggttcaacaa agcccatgac
    19201 gatcatttca gttgcttcag attcggtcag gcctcggctc atcagatagt agagttgttc
    19261 ttctgagatt tttgagactt tggcttcgtg ttctaaagag acttggctgt tgtggatctc
    19321 attgaatggg atcgtatcgg atttcgacca ttcgtccatg atgatcgtgt cacattcgat
    19381 atgagagatc gaacccgcac tgtttttggc aaaggttact tgtcctcgat agtttacttc
    19441 tccgccgtct tttgaaattg atttagagac gatagagcta gatgtatttg gtgcattgtg
    19501 gatcattttt gcacccgtat cttggatttg gtttgctcct gcaaaggcga tggagagcat
    19561 ggtgcctcgt gcaccttttc catctaagta aacacttggg tatttcatcg ttgtttttgc
    19621 acctaggttt ccgtcgatcc attcaacggt tgcgccttcg tatgcttttg ctcgtttggt
    19681 tactaagttg tacacattgt ctgaccagtt ttggatcgtt gtgtaacggc aatacgcatc
    19741 ttttcttgta aagatttcta cgatggcggc atgcaggctg ttacttgaat aagtaggtgc
    19801 cgtacaacct tcgacatagt ggatgcttgc tccttcgtca acaatgatca gcgtacgttc
    19861 gaattgcccc gtgttttccg cattgatccg gaagtacgtt tgtaatggaa catcgacttt
    19921 gacgcctttt ggcacgtaaa taaaggtccc acctgaccat acggctgaat tcagcgctgc
    19981 taatttgttg tcggttggcg gtacaagttt tgaaaagtac tctttgaata attcagggta
    20041 ttctttcaaa gcagagtcag tgtcagtaaa gacgattcct aacttttcga attcatcttt
    20101 catattatgg tacacgactt ctgattcata ttgtgcggaa gcaccggcta gataagctcg
    20161 ttcagcttct ggtataccaa tcttttcaaa tgtatctttg attttttctg gtacgtcttc
    20221 ccagtcacga gcgggtttgt cacttggttt ttggtaatat ttgatggcat cgaaatcgat
    20281 gtctgaaagg tcggggcccc atgtttgcat tggcattttg ttaaatgttt ctagagattt
    20341 cagacggaat tcaagcatcc attctggttc gcctttgatt cttgacatct cacggatcac
    20401 ttcttctgtc aagccttctc ctgtactgaa aaccggttcg acatcatcgt gaaacccgaa
    20461 tttatattct tctaattctg gtacgcccat gttctcactc ctcttccttg tggtaatgat
    20521 ggaaagctgc ggtaccatcg ttttccattg cttttcctag tgctttccag gcaagtgtcg
    20581 cacatttgat ccgagcggga aatttagcta caccaccaag catagcagca tcgcctaatt
    20641 ggtcgggatc tttgacttct tttccttgga caagctgtag aaagtctaca gttaaatgtt
    20701 ctgcttcttc tattgatttt ccgatgacgg cgtctgtcat catcgaggca cttgccgtac
    20761 tgatggagca gccactgccg tcaaaggcga tatttttgat gattccgtct tttacatcga
    20821 cttgcaattc gatcacatct ccacatgtgg ggttgttcaa ttcgatcttg cttgtcgagt
    20881 cagtcaagct tccacgatga tgcggatggg aagaatgatc caagatcact tgacggtata
    20941 aattgtctaa tttagatagt cccatgttgg aaaaactcct ttgttgccat gattgcttca
    21001 actaaacggt cggcgtcttg cgttgtgttg tagaaataaa agctcgcgcg agcagtcgct
    21061 gatacttcaa ggtacttcaa tagcggttgc gcacaatgat gacctgctct gacggctacc
    21121 ccttccatgt ctaaagctgt cgcgacatca tggggatgta acccatccaa attgaatgca
    21181 acgactcctg tccgaaaatt cgggtcttgt ggaccataaa cagtcaatcc atcaattgct
    21241 tgtaattttg gcaacacata agcggctagt tcttcttcat atcgatggat attgtccata
    21301 ccgagttttt gcaaataatc gattgcatgg cctaatgcga tagcgccggc gatatttggt
    21361 gtccctgctt cgaatttcca aggtagttct ttccatgtac tgtcgtataa ttcgacaaaa
    21421 tcgatcattt cgccaccaaa ttcaacaggt tccatttctt ctaaccattt tcgtttacca
    21481 tataaaacac caatacctgt tggtccgcac attttgtgtc cgctgaaagc aaagaaatca
    21541 cagtctagtg cttggacatc aatggccatg tgcggggcag attgagcacc atctactacg
    21601 atgaccccgc cgttttcatg gacaagttca gctaattctt gaacaggatt gaagacacct
    21661 aagacattgg atacatgagc aatggagacg attttggttt tgtccgtaat ctttttcgct
    21721 gcatcctcca tatccaggaa gccttcttct gttagttcga tatatttcaa gacagcacct
    21781 gttcgttttg ccaactgctg ccacgggata acattagaat gatgttccat gtaagagagc
    21841 actatctcgt ctcctgcttt gatatagcgg tcgccgaagg ttctggctac ccaattcaag
    21901 cttgtcgtcg taccacgagt aaacagcact tctgctgttt cttttgcatg gatgaacgcc
    21961 cgtacttttt ctcttgcttg ttcataatca tgggtcgcgc gttctgctaa cgtatggaca
    22021 ccgcgatgaa cattggcgtt atcgtgttcg taatagtacg ctaatgcctg gagtacttgt
    22081 tttggctttt gggtcgtcgc tgcattatcc agataaacta aaggttcatc attgacgtct
    22141 tgaaataaga taggaaaatc attgcgtagc tttttagcat cgatcatcca ttcaacttcc
    22201 cttcgattac ttcgactaat tctttccgaa cttgttctac agggattgct gttaagacag
    22261 aaccgaggaa tcctcgaatc actaagcgct ctgcttcttc tttatgcaat cctctactca
    22321 tcagatagta catttcttct ggatcaactc gtcctacact ggcagcatgt ccagcagtta
    22381 cttcgttttc atcgatcaaa agaataggat tcgcatcacc acgtgcttta tcagaaagca
    22441 tcaagacacg actttcttgt tgggcatctg caccttttgc ccctttcaag atgtggccga
    22501 taccattaaa ggtcaatgtt cctttttctc ggatcactcc gtgttgcaaa atatgcccga
    22561 tcgtatgagg ggctttattg gttactcgtg tatcgattcc ttggatttgt ctgcccgagc
    22621 tgattgccac gattttgact tctgcgtgtg caccttctcc tgctaaatct gagtcaaagt
    22681 cagcgatcac atgaccgtca ttcatgacac caattgccca gtcgacagaa gcatctcgta
    22741 aaatgtgccc acggcgattc atataagaag taatattttc tcctaattga tctacggctg
    22801 aatatttgat ttttgaacca gctttagcga tgacttccac gatgatatta cctgatgatt
    22861 ttgctacttg ttctttggtt gtctggaaac gttccagata agaaaattca ctatgttcat
    22921 ctgccacgat caacacatgc ttgaagaagt gctcatcgga tgcgccatct tgaatgaata
    22981 aggactcgag tggttcatcg atcacgacat ttttaggcac atacaggaaa ataccgctgt
    23041 tcataaaagc tacatggagt gcagtcaatt ggtcttcgtt catgtttact gcttttttca
    23101 tataatattc ttggactagt tctggatatt cgatcattgc agtgaacaag tcggtaaaaa
    23161 tgactccttt ttctgctaat tccacaggca gttgttcaaa aatcgtccat gaaccttgtt
    23221 ggacaagcac aggattgtct ttcattgcat caaatgccgg aatcgtgcct gtttcactga
    23281 tggtttgtgt ttctttcaca tcaaataacg accagcgatg gaatttcacc cgatcgatat
    23341 gcggcaatgg cagttcatct gctttcgcta gtgcgtcttg acggaaggtt ctcatccaat
    23401 caggttcgcc tttacttaaa gaaaaatcat ttaccgcgtc aagataatcg agccagtttt
    23461 tttctttcat tcttgctccc ccttacgctt cttcttcttt atagtcgatt cctaattctt
    23521 tactgatacc agcgtagcct tctgcttcca gacgtttagc taggtcagca ttccctgtca
    23581 tgactactcg gccttccatc atgatgtgaa cgacatcagg tgtgatatag ttcaataatc
    23641 gttgatagtg ggtgatgatc aatgcaccga aattgtctcc gcgcatttca tttactcctt
    23701 tagcaacgac tttcaaagca tcgatatcca gacctgaatc gatttcgtct aaaattgcaa
    23761 atgtaggttc taacatcaac aattgcagga tctcattccg tttcttttct ccgccagaaa
    23821 aaccttcgtt cagataacgt tcagccattt cttctggcat atttagtaaa gccatttttt
    23881 catctaactt tttcaaaaat gccataacag aaattttgtc gttttcatca cgtttcgcat
    23941 tgatcgctgc acgcatgaat tccgcattcg tgatacctgg tatctcactt ggatattgca
    24001 tagctaaaaa taagccaagt cgcgcacgtt catctacttc caagtctaaa acatttttcc
    24061 cgtcaaacaa aatttcgcct tctgttactt catagtttgg attgcccatg atagcggcag
    24121 ataaggtaga ttttcctgtg ccgttaggtc ccatgatggc atggatttct ccggttttca
    24181 ttgttaggtt tacgccttta aggatttctt tttcctcgat cgacacatgt agatttttta
    24241 tttctaagac agacatgcat tataccccca aattctcttt cgttctcact cattttagac
    24301 taattgagaa tgaagcgcaa tgaccttcct tactaaaatc aaatatcttt tatttattat
    24361 taaacaattt tgatttctaa attagaatag gtataaaaaa gatgagaaat aagccattac
    24421 agacttattt ctcatctttt ttgttccata ccttattttg catcagcctt tactggttta
    24481 acagagccgc cccatttgtc tgtgatccat tcttggactt ctttgcggtg aagcacttca
    24541 atcagttttt tgattttgtc actgtcttca tcacctttac gagtcgcaat aatattcacg
    24601 taaggagagt tgtccttttc aagcaatacg gcatcttcta atggattcaa ccctgcgccg
    24661 taagcgaagt ttgcgttgat tgcaactagg tcaccttcat cattttggta cgtagaagct
    24721 aaaagtgatg ggtcgatcgt atggttgaac ttcaagttct tagggttttc ttcaatatca
    24781 tcgaatgttg ctgtttccag atctactcca tctttgactt tgactaagcc agcatcttgt
    24841 agaattgtga tgatgcgtcc ccagtctgat tcagaatttg aagtaatgat tgttgcacca
    24901 tcttttaatt cggaaacatc tttgactttt ttagagtaca agcccattgg ttcgatgtgg
    24961 acagcaccgg cattgacaaa gtcatatcct ttttctttga tttctttgtt caaataagga
    25021 atatgctgga agtaattggc atcaatgtcg ccttcttcca aagattggtt cggtaataca
    25081 tagtcatcga atttgacgat ttcaagatcg attccttcat ctttcaataa tggtttcaca
    25141 tgttccaaaa tctctgcgtg cggtgtcggt gaagcgccga ctttcaatgt cgtgtcgtct
    25201 gctttatttg ctgaatcttt gctgtcgctg ttgttaccgc atgctgctaa tcctacagtc
    25261 agcaaaagcg ctgctgcaaa tccaaaaatt ttctttttca tatcctacat ccccctatat
    25321 cctattatct tttgtctgtt ttcttagtta agaaatcacc gattccttga acgataaata
    25381 cgattagtaa aataattaaa gtcgcaacga atgtcactgt tagattacca cgctggtagc
    25441 cttcctgcca agcaagacct ccaagtccac ctgcgccgat cgcgccggcc atcgcagtaa
    25501 atccgatcat cgagatcgtt gtaactgtta atccagaaat caatgcggga atactttcag
    25561 gtatcaagac cttccagatg atttcccagt aacttgctcc cattgcatct gctgcttcca
    25621 gtacaccagg gctgacttca cgaaaagcaa tctctactag acgggcataa aatggtgctg
    25681 cggataaaac aagagctgga atggctgctt gcgcacctag catagtgcct acgattgctt
    25741 tagtaaatgg aatgatcaaa accatcagga tcataaatgg cgttgaccgg aaaatattac
    25801 tgatgatcga tacgattcca taaatgattc gagcatacgg tttgtttttg cgtccaatag
    25861 agtaaaggat caatcccaaa actaatccga tgatcgtcac taagaccatt gagattaatg
    25921 tcatatatag tgtatcaagg atagcctgat tcatcgtttc cgggttgatt tgtttaaaat
    25981 ctagatatgt ttctgaaaag cttttatcca cgatggatca cctcaattcc tactccttgt
    26041 tctttgatga acacttctgc ttgatctaat tctttggttt caccagtcat caagacagtc
    26101 aatgaaccaa aggaaccttc tttcacttgt ttgatctttc catacacaac gttgacatcg
    26161 atgttatagc gtcgaatcgc ttgggaaatg actggctcgt tggcatttct ttcgctaaag
    26221 gtcaaagttg ccaaacggcc gcttggattt tctttgatga gttcagctaa tagttctgtc
    26281 gaatcagctt ctggttcgat gtcttgttgt acaaagcgtt tggttacttc ttttttcggt
    26341 tgtctgaaga cagtcaatac gtctccttct tctacgactt tccccaactc catcacagcg
    26401 actttgttgc agattttgcg gatcacattc atttcatgtg tgatcaagac gatggtcaaa
    26461 ttcaatttct tattgatcgc taacaataag tctaatactt cttctgttgt ttgcgggtcc
    26521 aatgcgctag tagcctcatc acaaagcagg atctcaggat catttgccaa tgctcgtgcg
    26581 ataccgacgc gttgtttctg tccgccggaa agttgtgaag gatatgcttg tccgcgtcct
    26641 tctaacccga ctaaacgcaa cagttcttct gcccgttctt tacgtttgct ctttggtacg
    26701 ccagctaatt ccagcggcag ctggatattt tcaagtacag ttcttgacca taacaggttg
    26761 aaatgctgga agatcatacc gattttcttt cggaatgtac gtaattcttt gttttttaat
    26821 tttgtaatat cttgatcgtt tacgatcact tcgccgctcg tgggaagctc aagtccattc
    26881 aggagacgga ctaatgtact ttttcctgcg ccagaatagc cgacgattcc ataaatatcc
    26941 cctttttcaa cgtttagtga aacgtcattg acggcttgga tgtttccgtt tttcccattg
    27001 aatgtctttt tgacattcgt tagttggatt aatgacatgc cattcacatc tttctcttat
    27061 gatttttcaa acaaaaaagc cttcgccctc ttgccttaag caaaaggacg aaagcttgaa
    27121 cttccgtgtt accaccttga tttaccgtta cttcgcaata acagcctctt ccagtacttg
    27181 gacttagctt ctttccgtat actgtcgcgc tgtaacgggc actcccgtag taagtttgct
    27241 tacgctcgct tactctgctc taagaccatc ttccatctca ttttcgttac ccattttcag
    27301 caaacagggc tctcttcaaa cgtccgtgac atgtactctt cttttcaaag ctttctcgtt
    27361 tcatatagga ttgcttatga ttatagcaac tggaagaatc agtgtcaact tttttcaagt
    27421 gattttctga ttttataatt cgtcaaattc ttttggatca acatctttga aacttaagat
    27481 ccacgcattc atttcatctg aatcattcaa gatattgata tcttcaccga ttttatcatt
    27541 aacagaagaa actgtacccg ttagcgggct ggcaaattca ttgaccgctt tttccgcttc
    27601 aacttcgatc agaggttcat ctttttttag cgattgtcct gcttttggca gattggcaaa
    27661 cgtgatgtct ccaagttctt cttgcgcttc atttgtcaaa ccaacacagt attcttttcc
    27721 attaaataag atccaaaggt tgtccttttt cttcaaatgc ttgcctgcca ttttatatca
    27781 gttctccttc ataaattttt tcattgaatc caattgcaac aagctggtcc tctcgtaaga
    27841 tcagtggtcg cttgatcagc ataccatctg tcgataagac ttggctcgct tttgaagccg
    27901 taaactcgcc tacttgatct ttcagtccaa gttcccgata tttcatcccg cttgtgttga
    27961 acaggcgacg gattggtact tgactcgttt ctagccaagt ctcaatcgtt gaggccttcg
    28021 gtacttcttt gatcatatca acggtttgat aactaacgcc atgctggtct aaccacgctt
    28081 tggctttttt gcatgttgaa catttcgggt accagtaaaa ggtgtacaat tttcttacct
    28141 ccttgaagat cttgcaggtg cttcattttg gtatctcatc gataatatca acccgatacc
    28201 gatcatgttc cctaaaatcg aggatcctcc ctgactaatg aaaggcaatg ggatccctgt
    28261 taaaggaagt aatccgatat ttgctccaat attctcaaag acatggaaca gcagcatcat
    28321 gatcaaaccg cttgcgatat aggcgtaaaa ttcattattc gtatcgaaac acacccgaat
    28381 catacgatag attaaaataa aatacaagaa aatgacgaat gcaccaccga taaagccgaa
    28441 gttttcaccg ataacggtaa aaatcatatc ggactcacga acaggcacat agatattgct
    28501 tacgttgaat cctttaccaa acaagccacc ggtaccaatt gccaaaagtc cttgggccgg
    28561 ctgataactc attcctgacg tatcatggaa agggtccagc catgaatcaa tacgtgcaaa
    28621 ctgataagat ttgaatccaa ctttataaag caagtctctt ccgccttcag tcgtaaccaa
    28681 gaagatcgtt cctgctccaa cgactactgc caagattact acaggtgcga tgatgcgcca
    28741 agagattcca gacatcagga aaatcccgcc aaagatcgca agaaagacaa gcatcgttcc
    28801 aaagtcatct tgggccatga tcaatgctaa aaccggtatt gtcacgatca agagcttccc
    28861 aatcagccaa aagtccgatt tcatcgtccg ttcttttact tgcgtattat gtttagttac
    28921 gatcagtgcc atcatcaaga tgtacgcaat cttcatcaac tctgctggct ggaaagtaaa
    28981 cgcaccgaat cgaaaccagt ttttagaccc tgttgaagca gctaaattct gatcatagaa
    29041 tttgagcaag agtcccatta ccactaaccc caaaccataa aggtaaggag tcagtttcca
    29101 gatccatttg gaattcaaat gcatgatgac aacgattgct gccgctccaa ctaaatacca
    29161 gacaacttgc atccctacgc ctttcaagac tgctggattg ttcggatcat ggctaagtgc
    29221 tacatataat gagagcagac caatcaaaca taacagaaag actggtaaaa tcacgccata
    29281 atcaatacga ttatcgttcg aagcttttaa ttttttttcc atgtttcatc ccttttctta
    29341 aaaaccatac tgctgattat tatagcggtc cagttagata atggaaagtc tttccataga
    29401 catttgggaa aaattaactt tccgtttgtg cagttgcttg agcaaatgca gtacaaaagc
    29461 ctactgctta tctttagtgt atcatagaac tttttttgat acatgcaatt atctaccagc
    29521 tgagattctc ctcgctgtga gaagtttctg tgctttcatt gtatcgaagg atctcttcgg
    29581 caatctctag cagctgatca gaaaccagat tggttcgttg gatgaaataa aaatatcgat
    29641 ggaaattcaa cggagtcatt ggcacatttt ctggaacaga gcgcttagac aaaatcgagc
    29701 gaccgattcc ttccttcagc aacgcaacaa ttacatcgtt acttttcacg atcatcttct
    29761 tatcttggat attcatctct tcaaaataac gctgcgtgta atgaaaaaca ccagagtttg
    29821 cttctcttac aagccacaat ttgctttttg ggtccccagc gatgactagc tggtcttcca
    29881 tcaaagagta tctttgtacg ccggaagtct ctaatggctt ttcgataaaa ccaaaatcgg
    29941 attcatgttt cgctacttgt tctaatgatt catgagaatt cgtgatttcg atctcaaaat
    30001 gcactttagg aaaccttttg atcagatgtt tcatcaactg aggcaaaaga tagacagcaa
    30061 aggtgtggga agcaacgatc cgacatactt cttcttcatt ttgttcattg agcaattttt
    30121 gctgagtttc ttcccattct tctaacaaaa ccagtgtccg ttcatataat aagtcggctt
    30181 gcttagttgg aagcatctct tttcttccgt tacgttgaaa aagatcgata ttcaattccc
    30241 gctccaactg tttgatttga ttcgagactg ctggttgaga taaaaacagt acttctgctg
    30301 ctctggaaaa atttcttgtt tcatatactg ctcgaaaagt cgttaataac ttaaacatgg
    30361 caccacttcc ataacttttt atgataatta ctataaaaac tatttgattt aaatatacaa
    30421 aaaaccatct ataatagaaa tgtacaacaa aacagttgct gatccaacta gtaacttaga
    30481 tgaagacaat cagctgtctg cttgctcgta atcatacatt cctattcatt ttacaatgta
    30541 ttcgttcatc ttggtactaa aatctattaa ataaaaagga gaatattata tatgaagaac
    30601 tttttgaaaa ttattccgat cccgatctgc gggttgatcc ttgggttagc ttcccttgga
    30661 aatctgttta aagactatca ccatgttgct ttaggcaatc taactggcgg tatctctatg
    30721 attcttatga tattcatttt tgttaaattg tttatcttat tcgaacacac gaaacaaacg
    30781 ttgcatgatc caatcatcgc ctccgtttca ccaactttta cgatgtcact catggttatt
    30841 tgtacttatt ttgtttcata tgaaagtatt gcaccaattg tcaaattcat ttggctgtgc
    30901 gctgttatct tccaagtgat ccttgtcttt tatttcaatt atcaccatgt tatcaaagct
    30961 gatctttcaa ttgaagcaat ttatccaagc tggtttatca tttacgttgg atttggggtc
    31021 atcactgtaa ctgccgggaa cttcttccca cttattggaa aaattttctt ttggatttca
    31081 ttgatctgct atgcgttctt attgccgatc atcatcaaac gtgtcttttt cgtaagaaat
    31141 atggctcatc cgacacgtcc gctgattacg atcgttgcag cacctggttc tctttgccta
    31201 acaggttatc ttaaaaactt tgctgaacct aatttttatc ttgtcgtagc attgttcgct
    31261 ttatctcaag tattgtattt cgttgtgctg tctatgcttc cacgtttgtt caagcttcgt
    31321 ttttatccaa gctatgcagc ctttacattc ccgcttgtta tctctgcaac agctatattt
    31381 actactgttc attacttcaa cagtttaggt atctatagta gcgtattgga tttcttaaaa
    31441 gttgcggaat gtctgtttgc aagtgtagtt gtcttctacg ttcttgctca ttacgtacgt
    31501 ttcttaatta aagaacatag caaacatgga aaatttgttt ctaaaaaaat cgcttaataa
    31561 tcgattcaat aggttgtgaa aaagctgttc tgcatcaagt aataagaacg gccaaacaaa
    31621 aacagctgtt catgtttttg tactggttgg gcttattacc gcagatgcaa gcttttgaac
    31681 accgtttacc aagaggtcgt aa
//
LOCUS       DACDYI010000030        31677 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.030, whole genome shotgun sequence.
ACCESSION   DACDYI010000030 DACDYI010000000
VERSION     DACDYI010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.030"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            261..1130
                     /locus_tag="IWM52_001323"
     CDS             261..1130
                     /locus_tag="IWM52_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAP9973582.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1339..2364
                     /locus_tag="IWM52_001324"
     CDS             1339..2364
                     /locus_tag="IWM52_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP9973583.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2403..3353
                     /gene="manA"
                     /locus_tag="IWM52_001325"
     CDS             2403..3353
                     /gene="manA"
                     /locus_tag="IWM52_001325"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAP9973584.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3447..5150
                     /locus_tag="IWM52_001326"
     CDS             3447..5150
                     /locus_tag="IWM52_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9973585.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5152..7185
                     /locus_tag="IWM52_001327"
     CDS             5152..7185
                     /locus_tag="IWM52_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9973586.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7196..7636
                     /locus_tag="IWM52_001328"
     CDS             7196..7636
                     /locus_tag="IWM52_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9973587.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7836..8963)
                     /locus_tag="IWM52_001329"
     CDS             complement(7836..8963)
                     /locus_tag="IWM52_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9973588.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8966..9643)
                     /locus_tag="IWM52_001330"
     CDS             complement(8966..9643)
                     /locus_tag="IWM52_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9973589.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9907..11673
                     /locus_tag="IWM52_001331"
     CDS             9907..11673
                     /locus_tag="IWM52_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAP9973590.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11688..12125
                     /locus_tag="IWM52_001332"
     CDS             11688..12125
                     /locus_tag="IWM52_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9973591.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12138..13289
                     /locus_tag="IWM52_001333"
     CDS             12138..13289
                     /locus_tag="IWM52_001333"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP9973592.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13351..14049)
                     /locus_tag="IWM52_001334"
     CDS             complement(13351..14049)
                     /locus_tag="IWM52_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAP9973593.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(14042..15394)
                     /locus_tag="IWM52_001335"
     CDS             complement(14042..15394)
                     /locus_tag="IWM52_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAP9973594.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15617..17017)
                     /locus_tag="IWM52_001336"
     CDS             complement(15617..17017)
                     /locus_tag="IWM52_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAP9973595.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17178..17915
                     /locus_tag="IWM52_001337"
     CDS             17178..17915
                     /locus_tag="IWM52_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAP9973596.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            18040..18519
                     /locus_tag="IWM52_001338"
     CDS             18040..18519
                     /locus_tag="IWM52_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAP9973597.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18516..19283
                     /locus_tag="IWM52_001339"
     CDS             18516..19283
                     /locus_tag="IWM52_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973598.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19513..20082
                     /gene="lepB"
                     /locus_tag="IWM52_001340"
     CDS             19513..20082
                     /gene="lepB"
                     /locus_tag="IWM52_001340"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP9973599.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20186..20788)
                     /locus_tag="IWM52_001341"
     CDS             complement(20186..20788)
                     /locus_tag="IWM52_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9973600.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20894..21363
                     /locus_tag="IWM52_001342"
                     /pseudo
     CDS             20894..21363
                     /locus_tag="IWM52_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            21469..22053
                     /locus_tag="IWM52_001343"
     CDS             21469..22053
                     /locus_tag="IWM52_001343"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP9973601.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            22056..23129
                     /gene="prfA"
                     /locus_tag="IWM52_001344"
     CDS             22056..23129
                     /gene="prfA"
                     /locus_tag="IWM52_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAP9973602.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23122..23961
                     /gene="prmC"
                     /locus_tag="IWM52_001345"
     CDS             23122..23961
                     /gene="prmC"
                     /locus_tag="IWM52_001345"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP9973603.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            23954..24133
                     /locus_tag="IWM52_001346"
     CDS             23954..24133
                     /locus_tag="IWM52_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973604.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24155..25180
                     /locus_tag="IWM52_001347"
     CDS             24155..25180
                     /locus_tag="IWM52_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAP9973605.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25355..26599
                     /locus_tag="IWM52_001348"
     CDS             25355..26599
                     /locus_tag="IWM52_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAP9973606.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            26752..27699
                     /locus_tag="IWM52_001349"
     CDS             26752..27699
                     /locus_tag="IWM52_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9973607.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            27833..28462
                     /gene="upp"
                     /locus_tag="IWM52_001350"
     CDS             27833..28462
                     /gene="upp"
                     /locus_tag="IWM52_001350"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAP9973608.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(28601..28843)
                     /locus_tag="IWM52_001351"
     CDS             complement(28601..28843)
                     /locus_tag="IWM52_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973609.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            28964..29036
                     /locus_tag="IWM52_001352"
     tRNA            28964..29036
                     /locus_tag="IWM52_001352"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28997..28999,aa:Thr,seq:cgt)
     gene            complement(29155..29793)
                     /locus_tag="IWM52_001353"
     CDS             complement(29155..29793)
                     /locus_tag="IWM52_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP9973610.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(29916..30548)
                     /locus_tag="IWM52_001354"
     CDS             complement(29916..30548)
                     /locus_tag="IWM52_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP9973611.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            30899..31198
                     /locus_tag="IWM52_001355"
     CDS             30899..31198
                     /locus_tag="IWM52_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP9973612.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT        10828 a         5491 c         6364 g         8994 t
ORIGIN      
        1 tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatag gaggagctat
       61 ttttgtttgg ctgttcttat tacttgatgc agaacagctt tttcacaacc tctttttata
      121 agagttttta ctcattaatt tacaaaaaac aagcaaaatg cgaattttct cacatttttt
      181 ttaacaaaat caaagttatt gcttgaaaga agaagaatcc atcgttaaaa taaagcttga
      241 cgtagagggg agcaagaaat atgaaagatt ttcaattggg ggctgccacc tatttaaaaa
      301 gtgtagccat tcgggtgaaa gaccgagata aaatgattga tttttacaag aacgttgttg
      361 gctttgattt gaaaagagaa gaaaacgaat tagccatttt aggaacagaa gaagctggaa
      421 gtgaaatgtt gctgctggaa gaaagtccta gagcgaatga tcacatgggg gtgatcaaaa
      481 agctgaaccg tttttcattg attatcccta gcacagaaga aatggcagat atcctttgtc
      541 gtatccgtaa aatgaattat ccaatcaaag aagctttaga agatcatgga cgtatgggga
      601 ttttgctcgt agacccagaa gagaatgaat tggaaattta ccatgaagag aataagaacg
      661 accaaagaga aattccagaa acaatggatc aagaagcatt attaagaaaa tctgaagaaa
      721 aattcagtaa actatcaaca ggttcttatt ttgaaaaaat ccatttaaat gttactaact
      781 taacaaaaga acgccaattt cttaatcatg tacttggttt gaaggtacat aatgaagaag
      841 agggcatcca tgtattgaat gaaggagatt tccatgttgg tttgacagaa gctagcggag
      901 gcgcaattga tttgcctaca gatgaagtga ttggacttga ttttctgaaa ttcatgatcg
      961 atactgaaag catggaagga ctgattgctc acttgcagca actatcagtt gatttcttct
     1021 tagacaaaaa gcacacagta ctcacaatct atgatgctat aggaatcgaa tggtggttcg
     1081 tcagtgagcc aaaagaaaag aataaaactg acattcgatg cgacgcttaa ctttttcttt
     1141 cttaacgatt gtcgcatgtc tcgtcatttt atgaacggtc atactatcgc ttattctaga
     1201 gatagaaaga gaaaatagta taccggggga aactgcgcgg tttttcgcgc agtttttttg
     1261 tgtaaaaaag gttgattcct catagattaa gctttaagct tggactgtaa gaatctgata
     1321 gaaaagcagg agaaaaagat gtatccacac acgaaacagc ttattttgaa aaagatggaa
     1381 gaaggatttt atccaggtgt agcagtcgcc ttcataaaag aaaatcaagt agaaacattt
     1441 acagccggct ttgcccaagt cgttcctgaa aagcagctga tgacagaaga tctgttgttt
     1501 gatgcagcct cactgacgaa agtcgttgca accacttcat tagttttacg tctattagaa
     1561 gatggaaaga tagaactcga tcaaccgctt cataaatatc ttcctgaatt tgaaaatcag
     1621 acaatcactt tgcgtcattt actgacacat acttctgata tcaaaacatg gataccaaac
     1681 agagataagc tatctcagga agaattgatc gccgcttatt tgaagctaga accaggtgag
     1741 caactaggaa aagaagtaaa atatacagat gccggtacga ttcttcttgg gttgatgcta
     1801 gaggaaattt accaagaaac tgcagtggaa atctttcaag aacaagtatt ggaaccatta
     1861 ggaatgatga acagcttatt tcttcctttt ccttccaaaa aaatcgttcc cacagaacaa
     1921 ctgccttcgg gtgaggtttt gcgtggacaa actcatgatc cgaaagcaag agttttggct
     1981 gaacatgcag gtaacgcggg tctctttatt gatttgaaag acagcattac ttttgtccaa
     2041 atgtatttga acaagggaaa aatccgtaat ggtcgttttt tagaagaaga gacgattgat
     2101 tctttactgc aagatcaaac accgaaaaaa aatggctatc gttctttagg atgggactta
     2161 aaaaaggatg tagaagacgg tcatgcgatt ctttttcata ctggttatac aggtacgttt
     2221 ttcttgattg atataaaaac acaagaagcg ttgatatttt tgtcaaatcg cgtacatcct
     2281 gtcgatcatc gtgcggagta tctcatatgt cgagatgaaa ttgttgaaaa ctttttgaaa
     2341 gagaaagcgg tattaccgaa ataatgctat aatagctatg aaaaatgaga ggagtttgaa
     2401 ttatgcagga acctatgttt ttaaaaccag tttttcaaga aaaaatttgg ggaggaagtc
     2461 gtttacgttc agtctttggt tttgatatcc ctaacgataa aatcggagaa gattgggcga
     2521 tcagtgcgca tcctcatggt gtcagtgtag tagaaaatgg cgaattcaaa gggaaaagat
     2581 tagatgaact atggtcagaa cacaaggaat tgtttggcca tccttcagaa ccagtgtttc
     2641 cattattgat caagatctta gacgcagaag atgatctatc tgtacaagta catccagatg
     2701 atgcctatgg gatgaaacat gaaggtgaat taggaaaaac tgaatgctgg tatatcatcg
     2761 atgctgaacc tggtgcagaa atcatctatg gtcatcatgc aaagacaaga gaagaactag
     2821 ctgagatgat caaagatggg cgctgggatg accttttgaa gaaagtacca gttaaaaaag
     2881 gtgatttctt ttatgtacca agcggaacga tccatgctat tggtaaagga atcatgattt
     2941 tagaaacaca gcaaagttct gacacgactt accgcgtgta tgactacgac agaaaagatg
     3001 cgcaaggaca aacaagagag ctgcatatcc aacaatcgat cgatgtaaca actgtccctg
     3061 cgaaaacacc agaattacaa atcaaagaag tccgaaaagg gaattcatcg atcgtcactt
     3121 atttagaaac tgagtttttc aatgtatatg agtgggatat taaaggaatc acatctttca
     3181 aaaaacaagc cccttataca ttgatgacgg taatcgaagg agcaggagac cttgtcgttg
     3241 acggaaaaac atatccatta gaaaaaggaa caagctgtat tattccaaat ggcgtttctg
     3301 agtgggcggt ccaaggtgaa ctagcaatca ttgcgtctga accaggcaaa tagttcccag
     3361 atagctgaaa gcagcagacg gtttttcgtt tgctgctttt tctacataaa atccgtccat
     3421 caataaatag aagtctggtg aaaaagatgg aatacaaatg gaaagcaaac aacagcagtc
     3481 gcgtgtctat gaaacaactg agaaaagctg gacatcactt aaatgaaatg atcatgccaa
     3541 atctaagtct gtttatcgct tggggaatct tgtcattagc aacaaaaggt gtgtctggcg
     3601 atttgcacac cacattagaa gaggtatgcc attggatgat ccagctgctt ttacccatct
     3661 tgatcagtta ttcaggcggg aaatcggtag ggggacaaaa aggggcagtc gccggtgcaa
     3721 ttgcttcttt agggttgatc gctcgttcca atgctccaca aatcgtagga gcgatgatta
     3781 tcgggccgct tgccggcatc gtatatgaac aatttgtttc tagattccat gtgaaattca
     3841 aggctggata tgaaatgctt gtcagcaacc tactagtagg actgactggt agcgttattt
     3901 gtataattgg aatcgttttt attgaaccag ttttaaatag tgttcacttg ttgctcgcct
     3961 ccgttgtgtc ttggctagtc acaatgaatc tgttaccact tgtcagtctg atcttagaac
     4021 ctctaaaagt actatttttc aataatgcaa tcaatcatgg tgtattgaca cctttaggaa
     4081 tcgaattctc acaaacagtc gggtactctt ccttattttt gatggaggca aatcctggac
     4141 ctggtttagg gatcttactt tctatccttt gttttgcaga aaaacaggaa aaagtaaatg
     4201 cgagtggagc attgatgatc catgcgattg gcgggattca cgaaatctat tttccatttg
     4261 tcttgctgcg accgtatcta tttttagctg taatggctgg tggggcaagc ggcacgttga
     4321 tatttcaatg gttagatacg ggattagctg cacctgtatc gcctgggtca ctactcgtga
     4381 tttttgccaa tgcgccttat ccacaactag ctggggtaag tatagggata gcaatcagta
     4441 catttgtgac cttcatttgt gcagcgatac tattgaaatt accaaataaa gaaaaagaga
     4501 acaaaacggt caaagaaagg ggagaaatct tggaaccatc tatcattcat tccgtcattt
     4561 ttgcttgcga ctcaggtatg ggatcaagtg ctatgggagc cgccctgctg aaaaagaaat
     4621 tagcagcaga aaaaatcgat ctttctgtcg gatacagctc tatctatgaa ttgaataacc
     4681 aagtaaatca gctggttatc gtgcaacatg aactagcggg tcttgcaaga gaaaaagtac
     4741 ctcaaagtca aatctatgta ctggatcact ttttggaaac agatatcttg ctgtcacgaa
     4801 taaaagaagc gatccaattt ccaaagcggg aagaaaaaaa agagatagca gatatcacag
     4861 aacaaaaaac gtcgcctgct aatattgtat ttttgtataa aaacaacaga cgcggctcac
     4921 aaactatggg catgacgatt ttaagtcaat tagcaaaaaa agcgaaaaaa cagtttgtta
     4981 tctcaaaaga atcaatagaa aaaatcaaaa tccaagaaga aacgatttat atcgcacaaa
     5041 gagaactagc tgtaactgaa cagctagaag aaaagatccc tcggctagtt ctagttgaac
     5101 attgggtagc tacgaatgaa tacgaaaaac tcgtgaaggg agaaatataa gatgtattta
     5161 tcgcccagag aaaaacaatt attagcagaa tttctcagta gcccgtcacc tgtatctatc
     5221 caaaaaatga tgaatttatt gaaggtcagt aaacggacta tttaccgcga attagataat
     5281 cttgaagtct cattgaaatc ggtaaatgct tctttgaaaa aagcagctag aggctcattt
     5341 gtcattgagg catcagaaga agtattagag caattaaaag aaaatacaga tgggaaaagt
     5401 aatgacttat ccactgctaa acgacaacac gcgattttag ccgagttgtt actgacgaca
     5461 gagccgttga gtcttgctca tttcatggaa tcatatcgca tcagtaatac cactttttat
     5521 gcagatatca aacagttgga agaaagtatt cgccaactgc ctttggaaat cattcggaac
     5581 cagggatacg agatcgaagg accggaaaaa taccgccggc ttctgacagc gaatgtatta
     5641 gaattagaaa tcaatgaata tgaacttttt catagtattt ctttcgatgc atcactgaat
     5701 tatttttttc gattcgttga tccgcaacat ctatctcttg cacgcaaagt aatcggggaa
     5761 gaattgatcc aaaaaaagac caatctaagt gatagaaagc ttgaacattt ggttttgatg
     5821 ctgacattga cgatggagcg tgtgacgaaa aatcatttac tgacaaatga gacatataat
     5881 agtctggcaa acaaagaatt actacagaca gctaaacgat tgttttcacg gatcgcttct
     5941 gagacaaaac aactttatcc agtaaatgag atcgtctttt ttgcaggatt attaaatgac
     6001 ttcacgaact catttgatca gaatttcttt gaagagacgt tcgatacaca gcttgcttat
     6061 ttagtaaaac aactgattca agaagtaagt gaagagacga atgtccattt ttatgaagat
     6121 gaaactttgt acaaaatgct gttgactcat ttatctggtg tgttttcaag agccgtttta
     6181 caagaagaga cactgaccaa tccgatttta gaaaaaatca tggttcaata cgaggaagtt
     6241 gcgaatgcaa tccgtaaagc agtagagcgg atttttcaag aaaaaaaatt atccgaagaa
     6301 gagatcgctt atatggtcct tcattttgcg aattcattgg aacgaagtcc gaaagaaagc
     6361 aatatcagta ttgcaggatt ttcgccaagt ggattagctt ctactagcat gttggaacta
     6421 cgattgaaaa ggtattttcc cttcatccaa caaattcatt tctatcggat tgctgattta
     6481 aaaaaaatcg atttacaaga agaatatgat gttgttattt ctacatctat tttaccagga
     6541 tacggtggaa aatatatttt agtctccccg ctactgctag aagatgaagt caagcagcta
     6601 aaagaagctt tttcgcatat tgagaagaaa aaaaggcaaa aaattacgta taacagcaat
     6661 aacgagtgga tgatagaaga tacttatgag gaaacaatgc atttcatgga tcaaatcaat
     6721 caattactcg gtcgtttttt tatccaagaa ctggataatc ctgagacgat ttccgaaacg
     6781 attcgcagct tatttcctta ttttcctgaa ggactagtaa cggatgaaga ggtggttcat
     6841 cgccggctgc ttaagcgata tgaacaagca cctatcggta ttcctcatac aaatatgggg
     6901 ttattccata catccagctc attgatttct aaaccgatct tttgtatttt caatttgaaa
     6961 catccgttga ccattgaagg gatggataga cagccagtcg atttgtcccg tatgttagtt
     7021 atgctggcgc cttcgccaat ccaagaaaaa gacgggattc ttttaggaaa gatcagtgga
     7081 gcaatcatca tgaatgactt gaacaccgag atcttccgct caggaaataa agaaatcgtt
     7141 tatcagttgt tagcgaaaat cctgattgaa gaaataaaga aatgaaggtg aaaagatgat
     7201 acagctacca gcagaaaaaa tattgctcga tcaatcctat caaaattgga aagaagcgtt
     7261 gaccgagatc ggccagtgga tgacccaaca agggggaatc aaaccggcat atatccaatc
     7321 aatgatcgaa agacaagaaa aagcgagtgt ctatatcggg aatttcgtag ccttgccaca
     7381 tgggactgac aaagatgaag aagtagtaga agaaggaatc tatttagtcc aagtgcctga
     7441 tggtgtggac tttggaacaa aagaggaacc aaaaatcgcg acactcttat ttgtagtggc
     7501 tttaaaaaaa gaacatcagt tagcggtgct ccaagattta gcctttttct gttcagacat
     7561 cgaccaagta atggcgctca gtgatgcgga aaccattgaa gaagtccaaa tgatttttga
     7621 acatggacat atataaccaa tgttcacagt aaaaaaagct gccttgcatc aagtaataac
     7681 ttcaaccaag ataaaaataa gaggagctat ttttgttggg aggttcttat tactgcggat
     7741 gcaagctttt gaaaactatt tattctagaa aaccgtttat ttgaaatagg ggacggtgac
     7801 aaggtttttg tcaccgcccc tattattttt gtgttttaat agctcttttt tatatagata
     7861 gtgcccatac ctatgaataa taaaacgata ctccatatac caagtgtcaa gataccattc
     7921 atgaaagtaa cctgttctga agctagtggc gagaaaatat ctcctcctat gatcacattt
     7981 tgaaaatcaa cataagaagt tggcaagtaa tgactgattg attctatcca tccaatcgaa
     8041 gaaaacaagc gatactctgc taagaaaaga caaagaagtg tacctaaaag attgactaga
     8101 gaatgttcaa ataaagcaga tagaagaaaa ctgattgtac ttaaaaagag cacccacaac
     8161 aacaggaaca gaaagctctg gaaaagatac tcatacatcg gcaggattcg tatctcctga
     8221 ccaatgatcg ttcctacagg ataacggaag tctcccaaac catttttgat tattactagt
     8281 atagagacga tcaaaaatgg catcacgaga cttagcatag ctgcaccata ataaatggta
     8341 aaacggatgc tatgtttctt taccatacta tccggtaata gattgactag agagattacc
     8401 tgctttcttt tttcataaga gatgatggaa gaaagaaaca aggcggttat acataaaatc
     8461 aaaaaactag aaatcattcc tgaaaaaata ttttcgtagt aatttgctaa agagagatgc
     8521 gctggcaagt caaaaataga atatctttgg atctgatgtt ctttcatata acggagcaac
     8581 tcgacacgtt tttgttgttc aatgattgga attccttgtc tttgaccaga aatcagtcga
     8641 tccagtatat ctttttcata atgataagtt gcttcgacca tttgctggac attccctgtg
     8701 ttttttgcat gtaagattgc ttcgatcagt ttgttgcttt tctctaaact ttccacaaat
     8761 tctttctcat tctcatcttc tttcaacgtt tcaatcaaac gttgcatctc ttcatagtta
     8821 tgattcagtt catcgaattc tgtataagca agtttatgat caaaaaaaag tgggaatgca
     8881 aagagcacga ccaacaatag aggaaataaa aagtttactt tgttttttag taagagattg
     8941 cgttcaaatg taagtaaaga catagttaca cctcatataa tttacgataa atagctaaaa
     9001 tctcttccat atttgaggat tctgtgacga tttctccatt ttttagaaaa catgctttag
     9061 aacaaatcga ttggatattc gcgatagcgt gggaagaaag aagaatcgtc ttcccctctt
     9121 tctgccattg tctgatgagg ctattcaaga tatcaatact tcctgggtct aatccgttca
     9181 acggttcatc gaataacatg acagatgaat cactgatcaa gtaagcagca agcaagacat
     9241 gttgtttcat tcctagagac atttttccaa tcggcaaagt aaggtactca gtcatcttca
     9301 atcgttggaa aacagtttgg cattctactt tgctctgcca atagtatttg atcaaagtca
     9361 aataatcaac agccgataga tagtcggata actgattcgt tgattctaga aaaaagaaag
     9421 accggcgata atctgtataa gcagtaggag agatgttgtg aagggacaat tgatcataag
     9481 ttgttggcag cagttgggca atgccattca gcaaagttgt tttgccaaat ccgttcgggg
     9541 caatcaaagc agctacttct cctgattcta ctaaaagatt caattgtcga atcacacgtt
     9601 ttttaccacg tttcatgttc atgtttttga tcagtaatgt catagatagg cctccaatat
     9661 aaaatttaat aacataatag aatggaaaac atctaaatta aatgctaaca aaagttattg
     9721 tttgcatatt ataaaaaaat taaaataaca gttttattta aaattgaagt tttctgatac
     9781 cgaaatcttt ttattttgct gacatactca aacctttggc acaaagtttc gtgccaaagt
     9841 acgactattt ctctttttag taagcggata caattagagt atcaaaagga gagagggaga
     9901 gacaaaatgg aacggacgat tcccaaaaac aacatttcgc taaaagcgag ggtacaaaaa
     9961 ttaggcagta ctttatcaag catggtcatg ccaaatattg gagcactgat tgcctggggt
    10021 gtattaacgg ctctatttat cccagatggt tatttaccaa atgaggcttt cgctaccatg
    10081 gtaagcccaa tgctaactta tctgattcct ttattgatcg gttatactgg cggaaaagta
    10141 attgctggtg accgcggatc agttgttggg gcaattgcaa cgatgggtgt gattgtcgga
    10201 acagatattc caatgatgct aggcgcgatg atcatgggac ctcttggtgg atatgccatc
    10261 aagaaatttg atcagctatt ccaaaaacgg atcaaatccg gttttgaaat gctagtcaat
    10321 aatttttcag caggtcttat cggttttgga ttagcgttat taggtttttc tgcgattggt
    10381 ccagttgtcg atgcattgac tcaagcaatg gcaaaaggtg tagagatcat tttatctgcc
    10441 catttgattc cattgacaag tatttttatt gaaccagcaa aaatcttatt tttgaataat
    10501 gcaatcaatc atgggatttt gacaccattg ggaactgaac aagtagcaga ggctggaaaa
    10561 tctattctat tcttgcttga agcaaatcct ggtccaggac ttggcgtatt acttgctttt
    10621 actttgtttg gcaaaggagc agcaaaatct tcagcaccag gagccatgat cattcacttt
    10681 ttaggcggga tccatgaaat ctattttcct tacgttatga tgaaaccgtt attattctta
    10741 tcagttatag gcggaggaat gtcaggaagt ttcattttcc aattgatgga tgctggacta
    10801 agagcgccag cttctccagg gtcgatcatt gcgattctag cgatgacacc gttcaattct
    10861 taccttcctg ttattttggg cgtgattgtt gccgcagctg tttcgtttgg tatttctgca
    10921 gtaattttga aagcggatgc gaaagataca aatgaagact tcgaaaaatc tgttcaagag
    10981 acaaaggcag ctaagcaagc ttccaaaggt gcccaaggta ttcctcaagg agctacattc
    11041 tctggcgtac aaaaaatcat ttttgcttgt gatgcaggaa tgggatcaag tgcgatgggc
    11101 gcttcgatct tgagaaaaaa agtacaggaa gcagacttgc ctcaaacggt gaccaatcaa
    11161 gcaatcagcc aactgacaga tgactcgaat acattgatcg ttactcaggt cgaactacaa
    11221 gaaagagcaa aacaaaaagc accgagcgcg cgttttgtct cagtagaaaa ctttttgaat
    11281 tcacctcgat atgatgaaat cattcaagaa ttaagcagca agcagaaaac atcagtttct
    11341 gaaattgaga atgagccagc agaagagttt ctttcagcaa acatcaaaca aatcaaagag
    11401 attctacttg tccacgatga tcgttccgga tcagcaacaa tggggatgac cgtgctcaaa
    11461 gatatattag acaagaacca tttaactatc ccgttaagaa aagtcaatat ccaagattta
    11521 aaagatgaac ctgattcact gatcgtagcc aaagaaagtt tagcggaaaa agtacagatg
    11581 gctgcacctc aatccattca tttatcagtc aacagcatgg tcaatccgca aaaatacgaa
    11641 tcgattgcac aatatttgaa acagacagct taagggagga aaacagcatg caaagtttgc
    11701 cattcgacat gatcgagctc aatcgatctt ttaaaaccaa agaagaagcg atccgctatt
    11761 gcggtcagat gcttgtagaa gcagggtgcg ttgatgagga atatatcgat gccatggtag
    11821 atagagacaa aatgttatct gtttatatgg ggaattttat cgcgattcct catggaacag
    11881 atgaagcaaa aaaatatgtg aaaaaatccg gcatctgtgt cgttcaagta ccagatggtg
    11941 tagattttgg taccgaagaa gaagaaaaga ttgcgactgt tttgttcggt attgcaggag
    12001 tcggagatga gcatttgcaa cttgtacagc aaatcgcact ttattgcagt gatatggaca
    12061 atgttgtcca gctagcagac gcgctcagca aagaagaagt aacggaaaat ctagcaattg
    12121 cttaaggaga gagaaaaatg agagcagtac attttggtgc aggaaatatc ggtcgaggat
    12181 ttattggaga ggttttggca gctaatggat ttgagattac tttcgtagat gtgaatcaca
    12241 cgatcattga tgctttaaat gaaagaaaag aatacgaaat cgaattagca gatgaatcaa
    12301 aagaaaaaat cactgtaaaa aatgtcaaag gaatcaacaa tcaaacaaat ccagaaaaag
    12361 taatccaaga aatcgctgaa gcagatttag ttactaccgc cattggcccg aatatcttac
    12421 cttttattgc tgagttgatt gcaaaaggta tccaagaaag agaaaaggaa ggaagcacaa
    12481 catctttaga tatcattgcc tgtgaaaata tgattggcgg ttcagcattt ctagaaaaag
    12541 aagtgtataa atatcttccg gaaacatcat ttactgacaa atatatcggc tttccaaatg
    12601 cagcagtcga tcgaatcgtc ccattacaac accatgaaga tcctttattc gtccaagtgg
    12661 aacctttcaa agaatgggtc attgacgata gccaaagaaa aaataaaaaa atccaattaa
    12721 aaggggtttt atatgtagat gatctagaac catatatcga acgcaaacta tttagtgtca
    12781 atactggtca tgcgactgtc gcttatacag gtgccttgtt aggttaccag accattgatg
    12841 aagcaatgca agatgcgtta gttgtggcac aattaaaatc agttttgcag gaaacaggaa
    12901 gtttgctgat tgccaaatgg gggtttgatg cagagcagca tcatgcgtat attgaaaaaa
    12961 tcattcatcg cttccaaaac aaaaatatct ctgatgcgat cacacgtgtc gctcggacac
    13021 cattacgtaa gttaggttat caagaaagat ttacacgtcc agtgagagaa ctacaagaac
    13081 ataacttgac ttgcccacat ttgacggcaa caatggggat tatttttaat tactatgatc
    13141 ctgaagatga acaaagccgt caattgcatg aaatgaagat ccatgaaaac ttggaacaat
    13201 tgattcaaga agtcacagga atcaatgatc cgaaaacaat cgggaatatc aagcaaaacg
    13261 tgaaccgtta tgccaagcaa gtcgcttaaa ataggatacg aaaaagggga atgtgacgaa
    13321 atttttgtca cattcccctt tttcgaataa ctaggctgtt tgtccttcaa gatagggcag
    13381 atccaattct ttaccgtaac gttgttctaa agctaggcgg atcaaacgag ctgccaggtt
    13441 ctcatttctt gcaaggatag gtccatggaa ataagaaccg aagacatttc ggtaaatgac
    13501 accttcagat tggtcttcac cattatttcc ttgaccttgt accactttcc ccaaaggtcg
    13561 ttcaccttca cctaagaaag tccgaccatt gtggttttca aatccataat aagtttcatt
    13621 gaattcttcg ttatggataa cgatatcacc gataaaccgg ttattttcct gacttaacgt
    13681 gtagtgatcc agtgcactga ttccttggat cttttctcca ttagctccca tgtagtagtg
    13741 acctagcaat tggtagccac cacaaatggc taacatgacg ccatcatttt cgatatagtt
    13801 gatcaaagaa tctttctttt tctggatgtc ttttgaaatg attaattgct caaagtcttg
    13861 accaccgcca aagaaaacca agtcatattt atcagcatca aaaggttcat gtatactaac
    13921 gatctccgta tggcaagaaa cccccatttt ttctgctata tatttcaaca tcaaaaggtt
    13981 tccgttatca ccatatgtgt tcatcaaatt tccataaagg tgggcgatat tgagttcata
    14041 attagccacg gttcattcct cctttgataa atccttgagc agctagttct ttacgaagct
    14101 gcaatacagc agtgtaagta gctaaaatat agacatgctc agttggcaat tctttgattt
    14161 tttcgattac ttctggtaat tcttttgttt ctgtcaattt attttctgga atcccggcaa
    14221 cttttagacg taaagtcata tctgtatgac ggtctcctcc agaaataaca gctgggatat
    14281 ccatatcggc aaagttttca aaattaccat cccaaatcca gctgacatcg attccgtctg
    14341 cgtaattggc atttaataaa gccactaaag aaaatggata aggagctaga ccaatcatat
    14401 cgattacttg attgatacct acaggatttt tgacaagtac taatgtacat tttttcccat
    14461 tgatattgat gacttcttgt cggccgaata ctttctcgtc atatcctaaa ccttgtttga
    14521 ttttatcagg atcgacattg aagtactctg ccactgcagt agctgctaaa gcattgtaga
    14581 cattatacat accgccaact tcgattccgt attcatgacc atcgatcacg aaatctgctg
    14641 acacattgtc catccgtacc atatcagtta atcgataatc caattcagga cgtttgaagt
    14701 cacaatttgg acagtaatat ttccctaagt ttgcataagt gatcatttta taatgcaaga
    14761 tgtgatgaca atttggacat aagacaccgt ctgtattgta atgagccatt tgttcacgat
    14821 cttcttcatg gtcaaagcca taatattttc taggattgac tgtttcgata gaattgaaga
    14881 ttggtgaatc accgttactg ataatcgttg catttggtga ctttgcggca ccgtctacaa
    14941 tcattttata ggtcgtatag atttctccat accgatccat ttgatcacgg aaaatatttg
    15001 taaataagaa cagttctggt ttcatatatt ctgtgacttt gctcaaactt gcttcatcta
    15061 tttctaggac agcaaacttt ttctgtcctt tttttggttt tgcctgcaag aaagtcgata
    15121 caattccctg taccatattc gcaccagtag ggttggttag aacttcatcg aattgctggc
    15181 gcaaaatatt gactgttaaa gcggtagtca atgttttacc gtttgttccg gtgacaacga
    15241 cgacttggta gtcttttgcc aaactatcta gtatatgtgg atcgactttc agcgctagtt
    15301 tacctggtaa acttgatccg cctttaaaaa aagtttttaa tgcccattgc gacgtttttc
    15361 caactgcaat cgcaaaatga ctgcgtattc ccattatgta acctccaaaa acgatttgct
    15421 gacgaataaa ttcaactaat cataccatat gttgaaaaaa aggttaaatt gcttttctta
    15481 ttcttaagaa aaggttacgg ctgaaagtgc atgaaatcgg acaaaatcgc atgatttaac
    15541 aaaaaaacag cttgaaacaa aatgattgtt tcaagctgct gtgattttat tattccctct
    15601 tacctatatt cccattttaa ccgataataa ttttctcgct tggatattca tagcctagat
    15661 ctcgtgtttc ttttggcacg aataacatca atgtatatag cattccgatc cgcccaataa
    15721 acatcagtgt agcaatcgtg attttgccca caacagacaa gtctccggta atccctaaag
    15781 ataaaccagt tgttccaaac gcagaagtta cttcgatgat gatcgagatc agcgtttgtt
    15841 cttctgtagc agataaaaaa acgatacaaa agaaacacat accaagagaa agcataaaga
    15901 caacgactga ttttcgcaca tcatcttgat cgattctccg tccaaaaata ttgatgttat
    15961 cttcactttt gaggaaggaa tatagataca aaccgatgat tgctacagta gttgttcgaa
    16021 tcccgccgcc tacagaacta ggactgcatc cgataaacat cagtaaagaa aaaatgatca
    16081 gtgttgtgat ttgaaaatca cccaaatcgt gaatctgcag accagcatta cgagtcgtaa
    16141 tcgaataaaa catcgaattg atccatttga cactcatatt ggaatcttgg aataaatggt
    16201 ccttttccaa tagataaatc aagacagttc ccgaaacaaa caagataaca aaagctaaca
    16261 cagctaattt ggtaaacaag gaaaaacgaa acggaagctt cgcattggaa cgtttgtata
    16321 atagccattc ccggcactcc atcaatacag gaaaaccaat tcctccgaca aaaatcaaaa
    16381 acatgatcag gatcaagaaa aaatagtcat gtgcaaaggg cttgatggaa tccccagtga
    16441 cgtcaaaccc agagttcgtg acagccgata tcgcctgata aaaaccgtaa aaaacggcat
    16501 cacgccaacg atcaaaatag cctctgtaat aaaaataaat agaaaagaat gtcccaaaaa
    16561 gtaattgaaa ccagattaaa atagcaaaag tgatccgaat cagtcggacg ataccagaca
    16621 aacgcggttg attcatatcc gtcatgatca gctgtcgctg tttcagtgtg atccgtcgtt
    16681 tagaaaaaat gacaaaagca gtagaaatca tcatgatccc caaaccacca acttgaaata
    16741 atatttccag cagtataata ccgttatcat tgaacacaga attgatatca aaagtcgata
    16801 gtccagtcac actcactgta ctgattgcca tgaacagcat gtccacaaaa gaaacatggc
    16861 tatctggttc gcgaaaaatc ggcatataaa atagaacgag tgataagacc gtcatcaata
    16921 tataataaaa aacgatgatt tggatcgatg agaaatgatt ggcagcaaac ttctgtcctc
    16981 gccgctgcag ccttcgaaag aataaatcaa catgcacagt actatcctcc tgacaatagt
    17041 cgtttctacc agtatacaag gggaattagg aatactcaaa gaaattatca aacagataac
    17101 aatacagctt tcgattaaaa tagaaatggc ttataataag gcaaatccta gaaaaaagaa
    17161 ggaggaggaa taacaaattg attaatgctc aacaaatcgt agaaaaaatg aaaaatcaaa
    17221 aaataaatta tgatacagtt ctgaaaaaaa tgatcatgca atgggagaaa gaagaagtaa
    17281 agcctaagat attaatacat acatgctgtg ctccttgcag tacgcacaca ttggaattca
    17341 tgtctcaata tgcagagatc acgttatttt tttcaaattc gaatattcat cctaaaagtg
    17401 agtatttgcg aagattacat gaacaaaaac gatttgtaga ccaatttaat gagaaaactg
    17461 accatcatgt tgcgctaatc gttgatgaat accgtccaaa tgaattcacc aaaatggttt
    17521 taacaaacca tcttgaaaac gaaaaagaag gtggtgctcg ctgcagtgca tgcttcaata
    17581 tgcgattaga tcttgttgcg caaaaagcgc aagaactagg atacgattac tttggaagtg
    17641 ccctgaccat ctcgccaaag aaaaatgcag ctttgatcaa tcagatcgga atggatatcc
    17701 aaaagattta cgggacacaa tacttgccaa gtgatttcaa gaaaaacaaa ggctatgaac
    17761 gctctttgca aatgtgcaaa gagtatgata tttatcgcca atgttattgt ggttgcgtgt
    17821 tcgctgccaa gcagcaagga tgcgatctga aagaaatcaa tcgggaagca aaagcttact
    17881 taaaaacatc aaccgtagtt tttgaggaaa attaaagaaa tctttcggaa aaacgtaaga
    17941 aaaaaataaa gaatttatgg tagaaataag gtagactgta taatttgtcc gtagtaaagt
    18001 aaggaaacaa gaataaattt caatgaagga agagataaga tgcgaaaagt agcacctatt
    18061 ttgaagccaa tcgctgtatt aacaatctcg acgattgtcg cgactactac aggtattgtg
    18121 aatagttcca gctaccgtgc accaagttca agttctagta ctgtagaaga aacaacacca
    18181 actacttcag tctcgacatc tgaaccaaca acaacggaaa gtttgaatga aaccactacg
    18241 acaagtacat gggacgatac aacaactagt tcgtcgtatg atgagacata tacaactgac
    18301 agctcaagcg aaacaagtaa tacggtacca gatggcactc catcggattc aaccaatcca
    18361 agtacaagtc cggatacaag tgctagctcc gaaacgatac caagcagtac gactgtagat
    18421 gataacacgc aaaatgatga tgcagcacgt acagcacagc aaaaccaaga ttcaggaaat
    18481 acccaaacgt ataaccagca agcaggtgat tctcaatgac atttttacta gaccttatca
    18541 acggggtgca taaattcctt ggctatttag acatcagtcc taaatatctt aaccgtggat
    18601 atacgatttt aagtgtgatt cccacgcttt atttattgag aattgtttat ggactttggc
    18661 aaaaccaaaa ctatcttcaa tttttcttat atggacttgc atttctagtg cttgtttatt
    18721 ttactgtttt aaatgtattt tattactttt tagataaaaa cacgaaagcc gatgtcactc
    18781 agctttttgt gaagtattta cctgatgaag cgttcaacat ccagacagaa gaatcgatag
    18841 ataatggaaa ttcgatcgat aaagtgaata cacaagaggt catggtttct tatgacgaag
    18901 attatcaatt gaaattagcc gagaatatgc ggtatttgat tggaaatggt gagatcaaaa
    18961 caaatgaact cggtcgcatg gatggatttc tggtcgatag aaatacattg tatccttatt
    19021 actatgtcaa gaaaacagga gagaaagagt atgttctaaa tattggacga agctactcgg
    19081 acatggaaaa agtagggacc attcgacttg actcaacaga agacacattg gaacctgtcg
    19141 gattgttcat cgtcggcggt gattttgtca aagacgggtt acgctatcat gagccttatc
    19201 gtctgaaatt gattgcaaaa aaggcacagt cgcaggaaga accagtggtt tatagtcgta
    19261 gccaaagaag aagacattca taatgtgatg aaacgccgtg tatcagtggt tgcgataaaa
    19321 acgtttattc ggaataaggg ggatgtgaca cgtcttttgt cacgttcctt ttttatataa
    19381 cgagaaagat aagacgaaca catcacttat tctttccttt catttataaa cattgtagaa
    19441 taaagaaagt acaacgaagt attgtgaaaa aacgtaataa ataacgagga aaatacaagt
    19501 aggaggtaag ctgtgaaaga atttttgaaa aattggggga ttttgatcct tgtattagcg
    19561 gctattttat tagctagagt gtacgtgttt acaccagtta ctgtaaacgg gcattcgatg
    19621 gaccctacat taagtgatgg acaacgattg atttcatcta agatctcaaa ctatgaacga
    19681 atggatatta ttactacaaa ggaaccgggg gatgaagaac ggatgattgt taaacggatc
    19741 atcggtatgc ctggagatac agtcaagatg gaaaatgacc agttgaccat taatggcaaa
    19801 aagtatgatg aaccttatct ggatgagttc aaaaaagaat tttcagaaga taagttacaa
    19861 ggagaatatg cttatagctc aggattccaa gcacaagcag aaagttcatc cacctttacg
    19921 agtgactttg aatatactgt gccaaaagga aagtatcttg ttctaggcga taaccgcttg
    19981 atttcaaaag acagccgaat gtttgggtta gtcgacaaag atatgatcca aggaaaagtt
    20041 gttttccgtt attggcctct ttcagaaatt aaaatcatgt gatgaaaaaa taaaaagaag
    20101 ctgtgacatg ttttttgtca cagcttcttt ttccgaaaat tccttcttat ttcttggagt
    20161 tgctcacttt ttttgcaatc tcattttaag ctttttggct gatggataag aaaagcgaag
    20221 cgtttttgtt cataaatagc ttttccccac gatggaaggt atgactagta gcagagacaa
    20281 agcccgtttt ttccaatcga ttattcaatt cttcttggct aaagccatta tggactttcg
    20341 gatgactaat ttgttcattc ttgtcaaagt ctacgatgat caattgaccg ccaggagcta
    20401 aaatttcata aagttttgta agaatattct ccgtatcagg aatgtgtaaa agtacaagtg
    20461 atagaaggat gagattagct tggacagcag gaaattcgac agaagcatca gcgtggatcg
    20521 ttcggacatt tttcagatct gctgcttgga tcttttcttc tgccattttc agcatagttt
    20581 ctgaagcatc ggcaatgatc agttctttga atctttccgc aagcggtaaa ctaacaagcc
    20641 cagttccgcc tccgtaatcc agcaggactt tttggtggct gtcgcgcggt agataagtag
    20701 ttagttcttc gcgaataatc ttggctaatg cttgacggtc aggggaatca taatggttag
    20761 cgatggaatc aaaaatattt tttgtcatca aatagctcct tacgtaaaac tttgaaattt
    20821 taacttatta tagcacgtga aaagaaagaa agcatgctat actcaattca aaaagggaga
    20881 atctagaaag aatatgccga tatcgtttgt aaaagataga gaggaaaaag gaaagtgtgt
    20941 ccgagaaata ttactagact tgcctgagtg gtttgggctt cctgaatcaa cggaaaaata
    21001 tatcgaggaa tcaagtaaac tacctttatg gtgtgaaaaa agaaaagaag aatatttagg
    21061 ctttatcact ctttcacaga caagtgaaga tactgccgaa atctattcta ttgtatggga
    21121 ataaaaaaag actatcatca ccaaggatta ggaacaaaac tttttaaaga agcagaaggt
    21181 tacgcagcaa aacattacaa atatttgcaa gtgaagactg tagacgaggg gcactactct
    21241 atatatgatc aaactatttg tttctacgaa tcattaggat tttcaaggtt ggaagttttt
    21301 ccaaatttat gggatgaatg gaacccatgt ttagttcttg tcaaaaaatt ggaacaaaag
    21361 tgagcaatag atttaatact aatctgtgct tgatgaaaat tctcactgta aaaaatgctg
    21421 aaaatcaagt ataataccat aggaacaaac tggaaggtga aaaagagcat ggcacaacta
    21481 ttttttaagt atggcgcaat gaatagcggc aagacgatcg agatcctaaa agtcgcacat
    21541 aattatgaag aacaaaataa gccagttgtc ttaatgacaa gcggtattga tacaagagat
    21601 ggtttaggcg ttgtttcaag ccgaatcgga ctgaaacgta atgcgatgcc gatatttgaa
    21661 gaaacagacg tatttgacgt aattcagaaa atggaaagca aaccctattg tgtgctgatc
    21721 gatgaagcgc aattcttaaa aaagaaacat gtcattgctt ttacgagaat tgtggacgaa
    21781 ttggatattc cggttatggc atttggtttg aaaaatgatt ttcgaaacga gttatttgaa
    21841 ggatcaaaat atttattgct ttatgcagac aaaatcgaag aaatgaaaac aatctgttgg
    21901 ttctgccaca aaaaagcaat catgaattta cattatattg atggaaaccc tgtttatgaa
    21961 ggcgatcaag tgcaaattgg cggaaatgaa gcttattacc ccgtttgtcg aaaacattac
    22021 tttcacccat caatgattac agaaggagaa taagcatgta cgatcaatta caagcaattg
    22081 aagaccgtta tgaagagctt ggagaacttt taagtgaccc agaagtgatt tctgatacaa
    22141 agcgttttat gcagctttca aaagaagaag caaacacgcg cgaaacagtc gaagtttatc
    22201 gtcgttataa agaagtagta caaggaatca ccgatacaga agaactgctt ggtgaaaaac
    22261 tggatgatga aatggctgaa ctagcaaaag aagaattatc tgaattaaaa aaagaaaaag
    22321 aagtcttaga agaaaaaatc aagatcttgc ttttaccaaa agatccaaat gacgataaaa
    22381 atatcatcat ggaaatccgt ggagcagcag gcggagacga agcagcatta ttcgctggcg
    22441 atcttttcaa tatgtaccaa aagtatgctg aagcacaagg ctggaaaaca gaagtgatgg
    22501 aagctaacgt taccggaatc ggtggatata aagaagtgat catgatgatc accggagaca
    22561 acgtatattc gaaattaaaa tatgaaagtg gtgcgcaccg cgtccagcgt gttccttcaa
    22621 ctgaatcgca agggcgtatc catacttcca ccgcaacagt tgtcgtgatg ccagaagctg
    22681 aagaagtaga gatcgatatt gaagacaaag atatccgtac agatatctat catgcttcag
    22741 gtgctggcgg acagcacgtc aataaaacag catctgctgt acgtttgact cacttaccaa
    22801 cagggatcgt tgttgctatg caagatgaac gttctcaaat caagaaccgt gaaaaagcaa
    22861 tgaaaatctt gcgtgctcgt gtgtacgata agatccagca agaagcacaa agcgagtatg
    22921 atgccaatcg aaaatcagca gtcggaactg gggatcgttc agaacggatc cggacgtata
    22981 atttcccgca aaatcgtgtg actgatcatc gtatcggatt aactatccaa aaattagatc
    23041 agattttagc agggaaatta gatgagatcg ttgatgcatt gatcatgtat gatcaaacat
    23101 caaaactgga agctatgcaa aatgggtaaa atgacctatc gagaagtcct gtcacgggct
    23161 tcttcttttt tagaggatca tgggaaagaa gcatatagta tccaattttt atttttggaa
    23221 cgaaaacaat ggaaaaaact tgattggctc cttcatatga acgaagagat ttcagaagaa
    23281 gaacagcggt tgatcgaaac cgatctgcga cttctgcttg cagatcatcc acctcagtat
    23341 ttgctaggat atgcagattt ttatgatcat cggttaaagg taactgaagc aacactgatc
    23401 cctcgaccag aaacagagga gttagtcgaa tggtgtttag acgagacgcc tggtgtaccg
    23461 ttagaagtga tagatattgg gacagggact ggagcaattg ctattagttt gaaagcagca
    23521 agaaaaaatt ggcatatatc tgctgtggat ctttcagaag aagcattaga agtagcgaaa
    23581 gaaaatgctc agaaagaagg aacgaaaatc agtttttatc atggagatac attaggacca
    23641 gtaatggatc agcaatttga tgtgattatt tctaatccgc cgtacatcag tcggaatgaa
    23701 tgggaactga tggatgaaag tgtccggtca ttcgaaccta aaatggccct ttttgctgag
    23761 aacgatggat tagctattta tgaaaaaata gcagttgaag caccaagcgt actaaaatca
    23821 aatggaaaga tttttttaga aatcggattt cgccaaggtg aagcagtaaa aaaaatattt
    23881 cagcaagcat ttccagataa aaaagtggcg ataaaaaagg atctttttgg caatgaacga
    23941 atgatccaag tttatggttg aagttgtgga tgaaaaattt tttatttgtt tttttattat
    24001 aaaaaaacac ttatttgaca aggatgtaaa cgactttttg gtttatcaac aagtttatcc
    24061 actattgaca aatagattat ccacaaacag tggaaaactt gaggataaac tcagacaaaa
    24121 taagaatgta tgaaaaaggg gttgtggata aactgtggaa acgaaacgat atgatgaaac
    24181 agaattgcaa gaggcagccg aagcactgaa agaaggagaa cttgttgctt ttcctaccga
    24241 aacagtatac ggattgggcg ccaacgctct tttaccaaac gctgtcaaga aagtgttttc
    24301 tgtaaaaggg agaccgcaag ataatccatt aatcgtccat gtcgcctctt ttgaacaagt
    24361 aaaagagtat gtggacaact ttcatccggc agcagagaag attgtgaaga acttctggcc
    24421 tggaccatta actttgatat ttaagatcaa aaaagacact ttgccctcag ttgtgacggg
    24481 aggattatcc acagctgcat ttcgtatacc ggataacaaa aaaacactag aagtaatcga
    24541 actttccggt gttccgcttg tcggtccaag tgcaaatacg tcgggaaaac caagtccaac
    24601 gactgcagat catgtatatc atgatctaca aggaaaaatt accgggatta ttgatgatgg
    24661 agctacgcga attggtgtag aatccactgt tcttgattta agtgatccaa cagccatgcc
    24721 tatgatttta cgaccaggtg cagtaacgaa agaacaaatc gaagcagtca tcgaatctcc
    24781 tgttgctatt gatcagcatc ttgtaaaaga aaatgaaaca ccaaaagccc ctgggatgaa
    24841 atacaaacac tactcacccg atacacgtgt tctgatggtc agagatggcg attggtcaac
    24901 tgcagttcaa tgggcaaaaa ataagaagat ccgtgcaggt gtcatagcaa gccccgaaat
    24961 tgcggatcaa gtgcggacag atactgcagc tgtttatatg tataacgaca actcagtaga
    25021 agcggcggct aaaggattgt ttgcaggtct aagaggatta gatgaaccaa cacttggatt
    25081 agatttgatt tttgtacaag tgtatccaga aactggattg gggaatgctt acatgaatcg
    25141 cttgaaaaaa gcagcaggac aaaattattt tgaaaaatga tcaattggtt attgtcgcat
    25201 tcttttagta tgatacaatg ctatcaagta aatcttctat cagtgaacga aaaactaagc
    25261 gtaactaatc gtaatagaag agatacgaat atttggcaat ttgtttttga tgggaaaaat
    25321 aaaattgtag gaaaataaaa aggagctgtt gtttgtggta gattacaaaa cgtttgaccc
    25381 tgatctttgg gctgcgatcg ccaaagaaga agaaagacaa gaacataatt tggagctgat
    25441 tgcctctgaa aactttgtct ccgaagcagt gatggctgca caaggaagta tcttgacaaa
    25501 taaatacgca gaaggttatc cagggcatcg ctattatggt gggtgtgaat ttgtcgatat
    25561 cgttgaaaat ttagcgattg accgtgcaaa agaattattc ggagcgaaat ttgcgaacgt
    25621 ccagcctcac tccggctctc aagccaacac ggctgcttat ctcgctttgg tagaaccagg
    25681 ggacacgatc ttaggaatgg atctttccgc aggcggacat ctaacacatg gatcaccagt
    25741 caacttcagt ggcaaaacct atcactttgt tgcttacggt gttgatccga caacagaagt
    25801 tattgattat aatgtggtac gtattttagc tagaaaacat caaccaaaat tgatcgtagc
    25861 aggtgccagt gcttacggac gaacgatcga ctttgcgaaa ttcagagaaa tcgcagatga
    25921 agtaggcgca aaactgatgg tagatatggc acatatcgca ggattagtag cagcaggact
    25981 tcatcctaat ccagttcctt atgcagatat cacaacgact accactcata aaacattgcg
    26041 tggccctcga ggcggcatga ttttgacaaa cgatgaagca ttagcaaaaa aaataaatag
    26101 tgcggtattt cctggtattc aaggcggccc tctagagcat gtgattgcgg gtaaagcagt
    26161 agcattcaaa gaagcattag atcctgcatt taaagaatat agtgaacaaa tcatcgccaa
    26221 tgcaaaagca atggtcaaag ttttcaatca agcaattgga accagagtca tttcaggggc
    26281 gacagataat catctgatgt tgatcgatgt tcgcgagtta ggtataaatg gaaaagaagc
    26341 agagagcatt ttggacagtg tgaatatcac tgtaaataaa aactcgattc cttttgaaac
    26401 actaagtcca ttcaaaacaa gcggtattcg aatcggcacg ccagcaatca ctactcgcgg
    26461 gttcaaagaa gaagatgctg taaaagtggc tgaactagtt gtcaaagcat tacaagcaaa
    26521 agatgataac gctcaactag acgaagtcaa aacaggtgta cgcgaattaa ctgaaaaatt
    26581 tccgttgcat aaaaaataaa aatttaaatt ccattagtaa acagaaaagt tcaatgttgg
    26641 gcttttctgt ttattttttg ggaaaacgga ttctttgaca atatcggtga tgctctctag
    26701 tatatgaggt gtaattttag tcttcacact aaatctcagg gaggaaaagt catgactgat
    26761 cgtatctcgt tagaaaagga agcagtagca ttcagtgatg caaatgcgcc tcatccgcgt
    26821 atctatgaac tagcaccgga agaaggaaga gttttactag aagaagtaca agaatcacct
    26881 gtgaacaaat attccgtcga tattgaagac ttgatggtgg atactggcga atggggaaaa
    26941 ctcaatattc gtctcgtacg accagaaggg aatatggata aattaccagt tattttttat
    27001 attcatggag caggctgggt ttttggcagt gctcaaaccc atgataaatt gatcagagaa
    27061 cttgcggttc gtacgaattc cgtcgttgtc tttccagaat atagtcgatc accagaagca
    27121 aaatatccaa cagcaattga acaaaattat gctgtgttgc aacagctgaa taaattagcc
    27181 aagtcaaaaa atctggatct cgagaatcta atcgttgcag gagattcagt aggtggtaac
    27241 atggctacag tcatgacgat cttaacaaaa gaacgaaagg gcctcccaat atccaagcaa
    27301 ttgctgtatt atccagtcac aaacgctgat tttgatacag attcctatca tgaattcgga
    27361 gaaaactatt ttttgacaag agaaggaatg atgtggtttt gggaccaata tacgacgaat
    27421 gaagaagagc gtgctgaaat cacagcttcg cctttgagag caaatataaa tgaactaaat
    27481 gatcttcctt cagcaatgat tttaacggga gaagcggatg tattaagaga tgaaggtgag
    27541 gcttatgcgc gcaaacttcg tgatgcaggg gttcctgtta cacaagtacg cttccaagga
    27601 atcatccatg attttgtcat ggtaaatgct atggatcaga caaaggcaac gcgtgcagca
    27661 atggcattat ctacttcttg gatcaagaaa aaagaataag ctttgcttgt ccgcaggaaa
    27721 acttcttttc aaaaacggat tttcttagga aaaatggcga gtttgactag tcaatcactt
    27781 gattttgttt tacaattaaa aagaaacgac agaacaaagg agagcttaaa aaatgggcaa
    27841 atttcaagtg atcgatcatc cattgattca acacaaacta actattattc gggacaaaaa
    27901 ctgcggaaca aaggtttttc gtgaagtagt cgatgaaatc gctatgttga tggcatatga
    27961 agtatcacgg gatatgccat tggaagacat cgtgatcgaa acaccgatca ctgaaacaac
    28021 acaaaaaacg ttgtctggta aaaaagtagc gattattcca atcttgcgcg caggtattgg
    28081 aatggttgac ggtatcttgg aattgattcc agctgctaaa gtgggacata tcggtcttta
    28141 tcgagatgaa gaaacattgg aaccacatga atatttcgtc aaacttccag aagacattga
    28201 tgcacgtcag ttgtttgtag tagatccaat gttggcaaca ggcggatcag caatcatggc
    28261 aatcgatgca ttgaaagaac gtggcgcgtc gaacattaaa tttgtttgtt tggtagcagc
    28321 acctgaggga gtaaaagctt tgcaagaagc gcatcctgat attgacatct atacagcttc
    28381 tctagatgaa aaattgaatg aacatggtta tatcgttcct ggactagggg atgcgggtga
    28441 ccgtttgttt ggtacaaaat aagtaaatac gaaaaagggt gtgacacaac ttttgtcaca
    28501 tccttttttg gtcattttta acgtttgatg cagaacagct ttttcacagc tttcgtttgc
    28561 atataaaaaa accatcccaa aaatgcagag atggtcaaat tcaaatcttt tttagttctt
    28621 tgtagttaac gattactaca cccttacttt gcgtcatcaa gttcgcattt tctgcagttt
    28681 catcaatctc aacaacagcg gaatttttca attgctttga aatcgttcca gtttgcgact
    28741 tgccgtgaat tgtgaacatt acgtgagtat tgatcttgaa tttttcacca atttccgatg
    28801 gtacgatagt tgtatcaaat tttccaaaag ttgccatttt cataaaacct gcctttcaca
    28861 tcaattatac cacgttttat gaaaaatatc atgaaattaa actaattttc catcggactc
    28921 tttacaatca gccaaataga gtatataatt tcttttggat aaggccagct tagctcagtt
    28981 ggtagagcaa cgcactcgta acgcgtaggt cgtaggttcg atccctatag ctggcattaa
    29041 aagagaagac agatatatca aggattttta atccattgat acatctgtct ttttgtttaa
    29101 aggaaataaa gtatgctttc ttagcaatag agcatgtatt atttctataa aaatttagga
    29161 gaataaaacg actagcaata tagctgtgaa atcaaatatc acatgaactg cccaacttgt
    29221 aatgatcgag ttgcttttaa tggcagcttg gttgaataac aatcttgcca ccccttgtat
    29281 caaaaggatg tgtgctacag tgtgtaaaat atttccatta tcataagtac tgtaatggat
    29341 aaatccaaag atgattgctg acaagaaact agcgacagga atgctgaact tactagaaaa
    29401 aatagcgaga aaatacactg agaacaattc ttctccaagt atcatgatag gcaaaataat
    29461 aaataataac gggctgaaag cctcatttat tggattgcct gttaaacccc atttcaaaat
    29521 ttgttgcaaa aataccgaga ctgcggcact gattactaca ttgataaaaa aatatttggc
    29581 tatatttttg acaggagcga caggtttcga gaacattttt ttcgtatctt ttttaccaaa
    29641 aacaagtgta aatgctataa atccagtgat agttaaagaa aaaatgctaa taatggatgg
    29701 cgcaaatagc aaccctaaca aaccgagtac aacgacaata gtgcccaata tccctttttt
    29761 aagttctatt ttttttccta aaatgttatt catgataata actcctacta ttcagttttt
    29821 gaagatagag tcagtataca tgaagagtta aaataggcaa tgttttttta tggatatttg
    29881 tattgctaaa aatttatctt atatattcat tttgattata ctacttttat ttgtaaaaat
    29941 tttctagcaa aaccaagtgc ttgcaactga ttaagcgtac aattttttag atgttccgag
    30001 cgtacttcaa tcatttcaaa ggagttgcta cttaaatcga tattttttaa atcagtatca
    30061 aaaaagctag cattttctaa atcattagta gaaaacatta ttttagtgag ggaacagtct
    30121 acaaaatctg cgcttgataa gacagagtgc tcaaaggtac tttcttttat gtttgaacca
    30181 gataaattta catagtccat tttacaattc tcgaataacg tgttcccaat ataactttcc
    30241 atcaatttag tacctgttaa tttacaattg acaaatttca cgcgatgcaa catgcttttt
    30301 gtcaatatac agcctgaaaa atcacagttt tcgaatatac agtcggtaat ctccattctt
    30361 tgaaaatcat tggaagcaaa aaatgcatac ttgaaaatag atttgtaaat aattggatgg
    30421 cagattattt ggttttcgat gacctctttt tgtgaaagga tattatacag ttcaaaatct
    30481 tcgttttcat agatatgaaa aaaggatcct ttattcaagc ttgcttcatt aactttagga
    30541 aaaatcatat ttaattacct ccattttcat aaagtcatat taacaaaagc taaaccaact
    30601 tacattagca gaaaatgaga taggttttcg cctttttcga taacgatagt gtcgctatct
    30661 cttgaaacgt tcttagaatt gccctgagag acacaaatta ggcgtaattc taataaaaat
    30721 cttagaataa aaatttttac tgatttaatt tagaatgttt ttaaaataaa aatcgttgcc
    30781 ataacagtgt attgaaaggt tttaacaatc cagtaatgat ttaaaaaaat ctagatgtag
    30841 cgtatacttt gtttatagaa tgtagtggga agaaatatat ttttgggagg aaatcgtgat
    30901 gtctaaaagt gaagaaatag aaaacatggt actaaaatta tatgatctcg ttttaaatcc
    30961 agagatcaaa gaaaaggaac gtatactttt gatagacgca aagacaggct tagaaaaagg
    31021 tcagtattat cctaaagtca taaataattt ggaacgtagt ttacgtccgt tagcaatcag
    31081 gggagaattg tctaaaccag tatcaccgtt ttatatggag atatcaacaa taggaaaatt
    31141 tgaaaaagaa ttaggtcgag gaatggcttc ggcaccgatt acttttgggc atttataaaa
    31201 acgagttatt catctcaatg aattaagaag tatagtcaat ctcataatgc aggctaggtt
    31261 catttatttg aacagagtct gtattttttt attctaaata aagaaacaga tgtttcttta
    31321 tattgtattt tatcctttaa ttaacacaaa aacagctagt ataaactaat aatgttaatt
    31381 aaaagtgtat tttatgaaac tatcgttatt tattaatgtt gcatttttgt aagttaaaaa
    31441 ttggaaatat agattgaaaa aatgaaagca ataagtaaaa taaaatgaat atatgcgaaa
    31501 aaataattta cattaggaaa aaccttgtaa aaacagtttt ttggcatatt tattttttat
    31561 aaatatttgt aaaataacta tcaatttttt agttaaaaga ttggaaatga gtgggggagg
    31621 aattgaagaa aggatttttg agagatgata tagtaggttt tatttttagt taaaata
//
LOCUS       DACDYI010000031        31333 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.031, whole genome shotgun sequence.
ACCESSION   DACDYI010000031 DACDYI010000000
VERSION     DACDYI010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.031"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            1..369
                     /locus_tag="IWM52_001356"
     CDS             1..369
                     /locus_tag="IWM52_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP9973613.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            549..1490
                     /locus_tag="IWM52_001357"
     CDS             549..1490
                     /locus_tag="IWM52_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAP9973614.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1559..2290
                     /locus_tag="IWM52_001358"
     CDS             1559..2290
                     /locus_tag="IWM52_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAP9973615.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2434..4614
                     /locus_tag="IWM52_001359"
     CDS             2434..4614
                     /locus_tag="IWM52_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAP9973616.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTELIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4634..5116
                     /locus_tag="IWM52_001360"
     CDS             4634..5116
                     /locus_tag="IWM52_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAP9973617.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5132..5893
                     /locus_tag="IWM52_001361"
     CDS             5132..5893
                     /locus_tag="IWM52_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP9973618.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6024..7358
                     /locus_tag="IWM52_001362"
     CDS             6024..7358
                     /locus_tag="IWM52_001362"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAP9973619.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7665..8150
                     /locus_tag="IWM52_001363"
     CDS             7665..8150
                     /locus_tag="IWM52_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAP9973620.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8143..8826
                     /locus_tag="IWM52_001364"
     CDS             8143..8826
                     /locus_tag="IWM52_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAP9973621.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8952..11597
                     /gene="polA"
                     /locus_tag="IWM52_001365"
     CDS             8952..11597
                     /gene="polA"
                     /locus_tag="IWM52_001365"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAP9973622.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11642..12478
                     /gene="mutM"
                     /locus_tag="IWM52_001366"
     CDS             11642..12478
                     /gene="mutM"
                     /locus_tag="IWM52_001366"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAP9973623.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12442..13071
                     /locus_tag="IWM52_001367"
     CDS             12442..13071
                     /locus_tag="IWM52_001367"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAP9973624.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13123..13455
                     /locus_tag="IWM52_001368"
     CDS             13123..13455
                     /locus_tag="IWM52_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP9973625.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIKEQRMKH
                     YTGIEEKL"
     gene            13767..14258
                     /gene="nrdR"
                     /locus_tag="IWM52_001369"
     CDS             13767..14258
                     /gene="nrdR"
                     /locus_tag="IWM52_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAP9973626.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14281..15654
                     /locus_tag="IWM52_001370"
     CDS             14281..15654
                     /locus_tag="IWM52_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAP9973627.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15654..16580
                     /gene="dnaI"
                     /locus_tag="IWM52_001371"
     CDS             15654..16580
                     /gene="dnaI"
                     /locus_tag="IWM52_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAP9973628.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16709..17149
                     /locus_tag="IWM52_001372"
     CDS             16709..17149
                     /locus_tag="IWM52_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP9973629.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17293..17994
                     /locus_tag="IWM52_001373"
     CDS             17293..17994
                     /locus_tag="IWM52_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAP9973630.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            17991..19112
                     /locus_tag="IWM52_001374"
     CDS             17991..19112
                     /locus_tag="IWM52_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAP9973631.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19127..20359
                     /gene="pepT"
                     /locus_tag="IWM52_001375"
     CDS             19127..20359
                     /gene="pepT"
                     /locus_tag="IWM52_001375"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAP9973632.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20491..21399
                     /locus_tag="IWM52_001376"
     CDS             20491..21399
                     /locus_tag="IWM52_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAP9973633.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21434..21706
                     /locus_tag="IWM52_001377"
     CDS             21434..21706
                     /locus_tag="IWM52_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAP9973634.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21717..22763
                     /locus_tag="IWM52_001378"
     CDS             21717..22763
                     /locus_tag="IWM52_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAP9973635.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            23014..25623
                     /gene="clpB"
                     /locus_tag="IWM52_001379"
     CDS             23014..25623
                     /gene="clpB"
                     /locus_tag="IWM52_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAP9973636.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(25774..26445)
                     /locus_tag="IWM52_001380"
     CDS             complement(25774..26445)
                     /locus_tag="IWM52_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973637.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(26438..26932)
                     /locus_tag="IWM52_001381"
     CDS             complement(26438..26932)
                     /locus_tag="IWM52_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP9973638.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(26952..28172)
                     /locus_tag="IWM52_001382"
     CDS             complement(26952..28172)
                     /locus_tag="IWM52_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9973639.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            28319..30154
                     /gene="lepA"
                     /locus_tag="IWM52_001383"
     CDS             28319..30154
                     /gene="lepA"
                     /locus_tag="IWM52_001383"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAP9973640.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(30248..31330)
                     /locus_tag="IWM52_001384"
     CDS             complement(30248..31330)
                     /locus_tag="IWM52_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9973641.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT        10432 a         5603 c         6713 g         8585 t
ORIGIN      
        1 atgtttacga atgaaataaa aatcatgctt tatgtggacg atgtagaaaa aaatgcaacg
       61 ttttggagag cagttggctt tgcggaaaaa gatcgtcaag aaatggatgg gacgcttata
      121 attgaagtag gagttgagga cagtcaaacc tcatttctgt tatatgatcg agcatttgtt
      181 gagtcccatt cacctggcac agctactagt gccccatctt tgatgtttta cagtaaagat
      241 atctttggac tatataaaaa aatgcagacg caatcagtca cattaggtga tatggtacag
      301 cttggagaag aatatgtctt taactttgct gatcccgatg gcaactactt tgcggttaca
      361 ggtaaataat agaaataaag actttgtttt tctgtgcgaa acaggaaagc gaagtctttt
      421 taattcaaat aataatatta tgtaaacaaa aaactttcca tgcgtaatta tacacaagca
      481 aataaatcgt caaatgcgaa cgccttcttt atactgttta ggaacgaaaa gaaggaagga
      541 agaaatacat ggattatcaa gtactgcttt attacaatta tacaaaaatc gaagaaccag
      601 aggaatttgc caaaaatcat ttagcctttt gcaaatcttt ggatcttaaa ggtcgtattc
      661 tagtagctga agaaggaata aatggtactg tgtctggaac cgttgaaaac acgactgcct
      721 atatgaaagc aatgaaagct gatcctcgat ttgctgatac gatgttcaag atcgatccaa
      781 gcgaaggaca tgcctttaag aagatgtttg taagataccg tccagagctt gtaagtttaa
      841 agctagaaga ggacattgat ccaaaccaag tgacaggtgc ctatctttca ccgcaagaat
      901 ttaaagaagc tattttagat gaacagacag tggtgattga tgcacggaat gattatgaat
      961 atgacttggg acattttcga ggggctgtac gcccagatat acgcagtttt cgagagcttc
     1021 cagactggat ccgcgagcac aaagaagaat ttatggaaaa acgggtagtg acctactgta
     1081 ctggtggcat tcgttgcgag aagttttctg gttggcttgt ccgtgaagga ttcaaagatg
     1141 tcggacagct gcatggcggt attgctactt atggtaagga tcctgaagtc caaggagaat
     1201 tatgggacgg caagatgtat gtgtttgatg aacggatcgc tgtcgatatc aaccaagtag
     1261 ataaacaaat cgtcggtcgc gactggtttg acggaacacc atgtgaacga tatatcaatt
     1321 gtagcaatcc tgagtgcaat cgtcagattc ttgcttccat tgaaaatgaa gaaaaacatt
     1381 taggagcatg tagcatagag tgtgcgaaac atccgcgcaa tcgctatgtt caagaccatg
     1441 gaatctctcc agaagaagca gaactgcggt ttacagcttt aattaaatag aaaacaatag
     1501 ttttgattgt cgaccatttc caatatgcgc tatactagcg ttgaggatgg tgatccacat
     1561 gaaatcattg atttatgcta ttggggatgt ccatggcaaa catgagcttt ttgaaaaact
     1621 tattcactat tatgatccaa agatccatca gcttgtttta ttaggggatc tcaatgatcg
     1681 aggtcctcgt acaaaagaat gttttttatt aggaaagcaa ttagtcgaag aaacaggagc
     1741 catttacctc cggggaaacc atgaagaata ttttttgcaa tttctgcaat caccggagga
     1801 ctggtatgcg ccttatatga gaaatggtgg aaaagaaacg tttgaaagcc tgattcaccc
     1861 aggggcatcg gaggaatact ccccaactga aatctcgatg atgatccaat cgagatattc
     1921 agaactgatt aaatttttag tccagcgccc cttgtattat gaatgggaaa attatctttt
     1981 tgtccatgcc ggtgtggatt tgacaaagaa agactggcgt aagacaagtc taagagactt
     2041 tatttggata agggaaccat ttcatgaagg gaaaaacaat acaggtaaga caattgtatt
     2101 tggacatacg attacaccaa tgctccatgg agatatgcag acaacagacc tctggattca
     2161 ggatcataaa atcggaatgg acggtggggc tgtattcgga gggtcattgc atggggttgt
     2221 ttttgacaag gaaggtattc ttcaagatat tgaatatcag aacaacgaag gaccttggca
     2281 tggaatctga ctaatggaaa gaatgaagga gctgtgactt tgttgcggct ctttttattt
     2341 ttatagagaa tattgtttct ttcagtagtt taacccagga taaaaagtat gatagaataa
     2401 agaagcaatt tttagcagaa aagaggcatt ttaatgacgg aaaatttgaa ccagacgatc
     2461 attcagcttg tacaaaaaga actttcagac tatcgtccta agcagttgac tactgtactt
     2521 aatttgttaa atgaaggaaa cactgttcct tttatcgcgc gttaccgaaa agaaatgact
     2581 ggaagtcttg atgaagtcca gattcgtgaa atcgaagagc ggtatgccta tcttgaaaac
     2641 ttagaaaaac gtaaaactga attgattcgg ctaatcgatg aacaagggaa actgactccc
     2701 gagttagaag cagaaatcac ccaagccgtg aaaatgcaac aagtggaaga tttgtatcgt
     2761 ccatacaaac aaaaacgacg gacaaaagca acgattgcta aagaaaaagg acttgaacca
     2821 ctcgcgaagt ggctgatgca gcttacagat ggagaggtgc aatctgaagc agaaaaatat
     2881 attgacaaag aaaaagaagt ctcttctgcc gaagaagcgt tgcacggtgc tcatgagatt
     2941 attgctgaac aagtcagcga taatgcaaaa tttcggactt ggattcggtc ttatacgtat
     3001 aacaaaggta tgtatgtcag caatgtcaag gatgagcaag cagatgaaaa aggcgtatat
     3061 gaaatgtatt atgactttgc agaacccgtc cataaaatgg tttctcatcg aatattagct
     3121 actaatcgtg gagaaaaaga agatattctg aaagtattct ttcaagtaga tgaagcagca
     3181 attttagctt acttggatcg ccaaatagtc aaaaatcctg cctctccttc ttcttcattc
     3241 gtcagagaag cctatcagga tagttataaa cgctttattc agccagcaat cgagcgagaa
     3301 ctgcgcaatg aattgactga aaaggcagat gagcaagcaa tcgctatttt cggcgaaaac
     3361 ctccgtaatc ttttgttgca accgccacta aaaggtaaag ttgtcctggg atttgatccg
     3421 gcttatcgta ctggatgtaa actagcagtc gtagatgcga cagggaaagt tttagcaatc
     3481 gaagttattt atcctcataa acctgcagcg caagcaaaaa gagaagctgc tggaccagct
     3541 tttatccaat taattaataa atatcaagta gatatggtag ctatcggaaa tggtacagcc
     3601 agtcgggaat ctgaactttt tgtggctgag cagttgaaat ccgccgacca taaaacctat
     3661 tacgctatcg tcaacgaagc aggcgcttct gtttactccg ctagcgaaat tgcacgaaaa
     3721 gaatttccgc atctacaagt tgaagaaaga agtgctgttt ctattgcccg tcgcctgcaa
     3781 gatcctttag ctgaattagt caaaatcgat cctaaagctg tcggagtagg ccagtaccaa
     3841 catgacgttt ctcaaaaacg tttggctgaa caattggatt ttgttgtgga aacagcggtt
     3901 aaccaagtag gtgtggatgt gaatacagca agcccgcagc tcttgcagca tatatcagga
     3961 ttgaataaaa caactgccca aaacattgtc atttatcgtg aagaaaacgg agaattcacc
     4021 gcacgaactc aattgaaaaa agtgccacgc ttaggaccaa aagcatatga acaagcaatc
     4081 ggattcttgc gagtccctgg tgggaaaaac attttggaca acacaggtat ccatcccgaa
     4141 agttattcta ttgcaaaaga aatattaact tcagtccaac tttcagaaaa agagcttggt
     4201 acagaagaag cagtagaaaa attgacacga ttatccgtag agaaactggc tgaaagccta
     4261 tcagtcggag aagaaacgtt agccgatatt ttagctgggc tgacacaacc tggacgagat
     4321 atgcgtgatg agatgccagc gcccttatta cgtacagatg tattgagtat ggaagacttg
     4381 aaaccaggga tggaattgac tggaacggtc cgtaatgtca ttgacttcgg cgcatttgtt
     4441 gatatcggtg tgaagcaaga cggacttgtc catatatcga aactcagcaa aaaattcgtc
     4501 aagcatccta cagatgtcgt gtcagtagga gatatcgtaa cagtttggat agaacaagtc
     4561 gatacgaaaa aaggcagaat cagtttgacg atgctttccc cttatgaaga gtaacaatga
     4621 ggagtaacaa aaattgacac aagaagaact gcagcagtta gtagaagaga tatcattaaa
     4681 aagcttccag cttcctttca agcaccaagc aatgttcaat ccacgcttac gtacaactgg
     4741 cgggcgctat catttaaacg atcatcattt agattttaat gcgaagctat ttgaagagtc
     4801 agacgaagag acgattgcag gaatcatcaa acatgaactt tgccattatc atcttcatct
     4861 agctggaaga gggtatcagc ataaagatat ggatttcaag tatcttttga aaaaaacagg
     4921 agggttgcgc tatgcaccag ccttgacgaa gaaacagata aagatggagt attatcgatg
     4981 ccaagattgt tctgccacga tctgccgcaa gagaagaatc gatgtacgca aatatcgttg
     5041 tggaagatgt catggagaaa tcaaatggat tgagacaaaa agagaagact ttaatgaagc
     5101 acaaaaaatc agatgaaagg atagaaggcc tttggaaaaa agtgagcaga ggaatctaaa
     5161 agactggaat cagctattaa attttttagc cgcacctgaa gagggaaatt ttgaggaact
     5221 gacggcacct acacttattt tagctggaaa ctgtcttcct atcttagctg ataaagcagc
     5281 acaaatgtat ttaaatggcc aagtagatca attgtttttg gtcggagggg tggggcatgc
     5341 gactagaatc ctttatgaga attttgaaaa gcaaggattt cattttgaag atggcatgag
     5401 tgaaagtgaa atctgtcgtc agtatctaaa agaagtctat gatttgcccg ataaggcttt
     5461 tctgatagaa tcgaagtcta caaattctgg agagaacgcc actttttcac tggagattct
     5521 tcattcctta ggagctgtac cagaaaaaat cttattgatg aatgatccga cactgcaacg
     5581 caggactcga gcaacttttg agaaagtatg gcaaaatgaa caaactattt tcgcgaatgc
     5641 tgtgccattt gtgccggaaa tccttcactt ttctgaagag atcatcttta cagcaaaaga
     5701 actgaatcat caatggccta aggaatattt ccatgcgcta gtattaggag aaatggaacg
     5761 tctccatgac gatgagaacg gctatggtcc gaaaggaaaa gattttattc ctcacattga
     5821 tatatcagag gaagtttggt cttcttacac cagaattaaa gagtcaataa aaacggattt
     5881 ttctcgtact tgaccatttt agcaagcgaa tcctcctttc caaataaatt tggttgaaat
     5941 tttcaatcgt ttataagata atgaggagcg tgttgaatgt aatagctaaa agaatggacg
     6001 ctagataaga gaagaggatt caaatggaaa atcaaaataa attgtatcat tttgttggta
     6061 tcaaaggttc aggaatgagc tccttggctc ttgttttaca tgaacaaggt ctaaatgtcc
     6121 aaggatcaga cattgaaaaa tatttcttta cacaaagaga tttagaaaaa gcaaatatca
     6181 cgattctgcc atttaacgca gataatgtaa aaccagggat gacgatcatt gcaggaaatg
     6241 catttcctga ttcacatgaa gaaatccagc gagcaaaaga attaggtttg gaagtgattc
     6301 gctatcacga ctttattggt cattttatcc aaaattatac gagtatcgct gtaacaggct
     6361 ctcacggaaa aacaagtacg actggactgc tctctcatgt attgagtggt gtccgtccaa
     6421 caagttatct aattggagat ggaacaggac atggtgatcc gcaagcggaa ttcttttcat
     6481 ttgaggcctg tgaatatcgc cgtcatttcc ttgcctattc accagactat gcaatcatga
     6541 ccaatatcga ttttgatcat ccggattatt atacaagtat cgacgatgta tttacagctt
     6601 tccaaacaat ggctggacaa gtgaaaaaag ctatttttgc gtatggtgac gatgcttatt
     6661 tacgcaaatt aaaagcaaat gtgccgattt attattatgg tgtcacagaa aacgatgata
     6721 tccaagcacg aaacatcgaa cgcacaactt ctggttctgc ttttgatgtc tatcatgggg
     6781 atgaatttgt tgggcacttc actgtaccag cattcggtaa acacaatatc ttaaatgctt
     6841 taggtgttat tgcagtagct tatttcgaaa aactggatct gaaagaagtt gcagaagaaa
     6901 tgctgacttt cccaggagta aaacgtcgat tcagtgaaaa gatcgtagca gacatgaccg
     6961 tagtcgatga ttatgcgcat caccctgcag agatcaaagc aacgattgat ggcgcacgcc
     7021 aaaaatatcc agataaagaa ataattgcag ttttccaacc ccatacgttt acgcgtacga
     7081 ttgcattgat ggatgaattt gcagaagcac ttgatttagc tgataaagtt tacttatgcg
     7141 atatttttgg ttcggctcgg gaagaacaag gaaacgttaa gattgaagac cttggagcga
     7201 aaatcaaaaa aggtggagaa gtcatcaaag aaaataatgt atctccattg ttagattatc
     7261 atgatgcagt agtaatcttc atgggtgctg gagatgttca aaaatttgaa caagcgtatg
     7321 aaaaactttt aagcagcacg acgaaaaatg tactgtaata caattactgc agtgtcctta
     7381 taaaaataga aagaggggga gtggcaaaaa tcttgccaag cccccttttt tgcaacttat
     7441 gataaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaatcaaat caaaaacatg
     7501 agaagctgtt ttttcttggc tgttcttatt atttgatgca ggacagcttt ttcacgacct
     7561 ctgtaaacta taggaatctg ttaaatcaag ggagcagaac ttgcctttta cgctactttt
     7621 ggtacaataa tgtatgatta aaaataaact ggagggtttc tatcttgaac ataggtaagc
     7681 cgagaaaact aggaaaaaca ataaatgaaa tcgaggaagg cgactcgtta tccttaacag
     7741 aatctattga agataaccag cttttgctgt atttgggatt gacgaatgat gcaaatcctt
     7801 tgtatatcca acatgacttt gcacaaaaaa cagaatatca acagccaatc gttccatcag
     7861 tgatgctgat ggggatcatc acaagtgcaa tttcaaagca tcttcctgga ccaggatcac
     7921 atgttgtcaa tttttctgtc aattttattg aaccggtttt ccattatgaa acactgactt
     7981 ttcagtttga ggtcataaaa gtagataaga tgaaggatgt ggtgacgatt tctgtagaag
     8041 cggagaatac gaacgaagat cgtgtgttgg atgctgtcgt tatggtccaa ccgccacaag
     8101 ttcctgaatt ggaaatagca atcgaaagtg aaggtgaatt gaatgaataa tccaagtaca
     8161 tcaatgtcag gtatcaatcg tttttatgca aaagtttatg gatttttagg tatgggactt
     8221 gcccttagtg cagtcacttc ttacttggct ctttgggtat ttcctttgca attagcaact
     8281 tttatcaata attttccact aggctttgtg ggtctgtgga tcgttgaact gattttagta
     8341 atggttctag gcgtaaaagc acaaaagaat ccgagcttag caatcggtgg atttattgcc
     8401 tactctcttt tgaatggcat aacattagcg gttactcttg catactacga tattggaaca
     8461 gtaacgcaag ctttcttgac agctactttg atgttcatgg ggatgtctgt tgtcggagta
     8521 acaacaaaaa gagatcttag cggggttgga agagcaggac aggcagcttt aatcggagtg
     8581 atcattgcac tagtcttgaa catcttcatt ttgagaagta gcgtagttga gctgttcatc
     8641 tccctactgt tagtcgtcat ctttgcaggg attacagctt atgataacca aagcatccgt
     8701 aatatctatg tccagacaaa cggaacagca catagtggaa ttgctgtatt catggcattg
     8761 aagctgtatt tggactttat caacttgttc ttggcgtttc tgagaatctt tggacggaac
     8821 aactaaaaaa gaaaaaaatt catcagttgg ggctataccg aattatttgg tatggtcccg
     8881 ctttttcttt tcgcattccg caggattctt tgttagaatg aataagaact ttacagtaag
     8941 gagttaagaa gatgacaaaa aataaattat tactcgttga tgggaacagt gtggcttttc
     9001 gcgcattctt tgcgcttcac aattctcttg aacgtttcaa aaatagaaac ggtcttcata
     9061 caaatgcaat ctatgcattc aatacgatgt ttgaaaatgt tatgcaaaaa gagcaaccga
     9121 ctcatgttct ggttgcgttc gatgcaggaa aaacgacttt tcggacagaa atgtatgcgg
     9181 agtacaaagg tggaagatcc aaaacacctg gcgaattcaa agaacaaatg ccatatatac
     9241 gtgatttgtt gacaggacta ggggtacagt attacgaatt gcccaattat gaagcagatg
     9301 atattatcgg tactttggca gaaaaagtag acaaagatca atttgatgtc gtcgtgcttt
     9361 caggtgatcg ggatttgact caattagcta gtaaagaagt aaaagtggat atcactgtaa
     9421 aaggtgtcag cgatatcgaa agctatacac cagaacatgt agcggaaaaa tatgatgggt
     9481 tgacgccaaa acagattatt gacatgaagg gactagccgg agatgcctct gacaatatcc
     9541 ccggtgtgac taaaatcggt gaaaaaacag ctatcaagct attgaaacaa tatggctcag
     9601 tcgaaggaat ttatgaacat atcgatgaaa tgaagcaaag caaaatgaaa gaaaatctga
     9661 taaatgacaa agagcaggct ttcttatcga aaaaattggc gaccatcaat acttcagcgc
     9721 ctgtggatgt ctctatcgac tcattgaagt atgaagggaa aaatctcgat aaactcgttc
     9781 cgttttataa agaaatggat ttcaatcagt tcctttctaa attgaatatc gtagaagaag
     9841 aagtaaaaat ggatgatatt ttgtttgaag tcgttcatga attcaaagag gaaatgttta
     9901 ctacggatat ggcgttgtac gtggaaatga tgggcgacaa ttatcataca gaggaaatcg
     9961 tcggagtagc gtgggggaca gaaaagaaaa tttatgtcac gaacgatcta tctgtattcg
    10021 aaaaccgagc gttccattct tggatcactg atccgactcg gctgaaaaaa gtatatgatg
    10081 cgaaacgaac ttatgttgct ttaaaccgct atactggaaa aacgaaagga atcgattttg
    10141 atgtcttgct aggtgcatac cttctcgata cgaatgaaaa aagcacagat atcgaaggaa
    10201 tagctgctca ttacggatac aacgatatcc aatcagatga agctgtatac ggcaaagggg
    10261 cgaaaaaagg tttaccagaa gaagaggaac tcttttttgc tcatttagcg cgaaaagtag
    10321 ctgcaatcaa tgcgttgaca ccaaaattga gccaagaact ggcagaaaaa aaccaagaag
    10381 atcttttccg gaaaatggaa ctgccattgt ctatcatttt ggcagagatg gaaataagcg
    10441 ggatcaaagt agatgcgact cgcttacaag aaatgaaggg cgagttctct gctcgcttaa
    10501 gagagatcga acaaaaaatc tacgaagaag ccggcgaaga attcaatttg aattcaccaa
    10561 agcaattagg agtgatctta tttgaaaaaa tgggattacc cgttatcaaa aaaacaaaaa
    10621 ctggatattc cacagccgta gatgtactgg aacagcttcg tgaacaagca ccaatcgtag
    10681 aggatatcct cacctatcgt caaattgcaa aaattcaatc tacctatgtc gagggactgc
    10741 tgaaagtgat ccaatcagat ggaaaagtgc atacccgtta tgtccagaca ttgacccaaa
    10801 caggacgatt aagctctgtt gatcctaatt tgcagaacat cccgattcga ttagatgaag
    10861 ggagaaagat acgtcaggca tttgttccta gagaaaaaga ctggctgatt tattcttctg
    10921 actattcaca aatcgagtta cgcgtacttg cgcatatctc aaatgatgaa catttgaaag
    10981 ctgccttcct agaaggtcaa gatatccatg ccagcacagc gatgcgtgta tttggaatcg
    11041 aaaaggcaga ggatgtcaca ccaaatatgc gtaggcaggc aaaagcagtg aattttggta
    11101 tcgtgtatgg catctctgat tacggacttt cacaaaatct aggaatcagc cgaaaagcag
    11161 cacagcaata catcgatacg tactttgaaa aatacccagg tgtcaaagaa tatatggaaa
    11221 gaatcgtacg cgaagcaaaa gaccaaggct acgtcgaaac gctgtatcac cgccgtcgat
    11281 atctgccaga catcaattca agaaattaca atatccgttc ttttgcggaa cgaacagcta
    11341 tcaatacacc aatccaagga agtgctgctg atattttgaa aatcgcgatg atcgaattgg
    11401 ataaacgatt aaaagaaaca ggtcttcaag ctacgatgct gttacaagtt catgatgaac
    11461 ttgtcttcga ggtaccggaa aaagaacttg aatcattaga taaattggtg aaggaagtta
    11521 tggaacaagc tgtttctctt catgtaccac tgatcactga cagcagttgg ggaaaaacgt
    11581 ggtacgaagc aaaataaaca ttgtttaaga cagagcaata taatgaaggt gaaaagaaaa
    11641 aatgccagaa ttaccagaag tagaaaccgt tcgtaaagga ttggaacggt tagtagtagg
    11701 aaaaacgatt caaaaagttc aggtcttgtg gcccaagatc atcgagcaac cagagacacc
    11761 catctttgag gcttctcttg ttggcgaaac catccaatcc attggacgta gagggaagtt
    11821 tttaattttt catctcgatc attgtgaact gatctcacac ttgcgaatgg aaggaaaata
    11881 tcaatttacg aaagaaaata caccaatcga taaacatacg catgtacttt tcttttttga
    11941 ggatggaagc caacttcgct acaacgatgt acgaaaattt ggtcgtatga caatcgtcga
    12001 aaaaggagca agtgcaactt accgaggaat catgaaattg ggacctgagc ctttgccaga
    12061 tagcttctta ttagcagatt tcgcaaatgg actgaaaaag tcccacaaag caatcaagcc
    12121 attgctgcta gaccaacgtc tagtgactgg tctaggtaat atttatgtag atgaagcatt
    12181 atgggaagcg aagatccatc cagaacaacc ggcgaacaca ctgcgcccca aagaggtaga
    12241 acaattacgt ctatcgatca ttgatgtttt ggatcgtgca atagaagccg gaggtaccac
    12301 gattcgcagt tatttgaatg cattaggaga atcaggcgga tttcaagtag cactgcatgt
    12361 ctatcagcaa actggcaaac catgtatacg ttgtggaacg ccgatcgtga aaacaaaagt
    12421 ggcacaaagg gggacgcact attgtccgaa ttgtcaaagg ctaaaaatcg caaagtagga
    12481 tttgttttag gtttgacggg tggaatagct accggtaaaa gtacggcagc caaagtcttt
    12541 cagtctcatg gatttccttt gatcgatggc gatgtgattg ccagagaaac tgttgagcct
    12601 ggaacgcttg ctctgaggaa aattgtatca gtgtttggcc aagaggtatt gcagcctgat
    12661 gggcaactag atcgagggaa acttgggatg attgtttttc catcaaagga aaaacggcaa
    12721 aaacttgatc agctattaga tccgtttatc cgaaaagcga taaaagatca aatcgcctct
    12781 ctaagttcca atcatccttt agtaattgta gatatcccat tgctctacga aggtcattat
    12841 gaccactata tggatgcagt agctgtcgtt tatactacgc cagaaactca gcttcagcgt
    12901 ttgatgaaac gtaatcaact gacgctagaa caagcgcagc agagaattag tagtcaactt
    12961 ccaatcgaag aaaaaaagca gcgtgcggat attttgttcg acaacaatgg aacaaaagaa
    13021 aaacttgtgg gacaaatcga aaattggctt tttgctaatc gctttctttg agtatctgtg
    13081 tcaattttgc tatcctgagt atatagaaag tgagggatag cgatgggatc attaaaaaat
    13141 caaaaggata aagtcgtagg taacgcaaaa gaaaaaatcg gcgagatgct caataaagaa
    13201 aatctaatcg atgaaggtcg tacgcaatca gaaacagcag aagaaaaaga aaagcagtac
    13261 caagcaagaa tcgatgaaat agaagaccag caggcagaag aagaagcgaa gcggcaagat
    13321 ttgactcaag aacgaaaaaa tccgacacct gatcgtagta aaacaccgct cccagaaagt
    13381 gaccacattc ccgatgagca aaaaatcaag gaacagcgaa tgaaacacta tacaggaatc
    13441 gaagagaagt tataaaatag taaaaaatcg agatgtgact tgaagcagat gagaaggtgt
    13501 tcatctgctt caagtttttt gtgctagatg actaaatcag cttttgtgta agttatttct
    13561 cagtaatcaa ggtgatcagt attggaaaat tgaaatttag taaggacagg ggacacaatt
    13621 acgcatgaaa gtagaaaaaa ttgttcttat atccgtgttt tttgggtagg acagtcgcgt
    13681 ttgtgatttg tttcgtgtta taatacaact agttgtaaag aaagacacat cctttatata
    13741 aaatgcgatg caagaggtga gacaaaatga gatgtcccag atgtcaacat aataattcac
    13801 gagtaatcga cagccgtcaa gcagatgatg gacgtgcgat ccgtcgccgt cgcgaatgtg
    13861 agaattgttc gttccgattc actacatttg aacgaattga agctgctccg ttattagtaa
    13921 tcaagaaaaa cggggaacgc gaagagttca atcgcgataa aattttaaga ggactcatcc
    13981 gttcagcaga aaaaagacct gtagctatgg aacaaatgga acaaatcgtc aaccatgtcg
    14041 aaaaccgcgt acgcagtctc ggagaaaacg aagtatcgac gaatctaatt ggtgaatatg
    14101 tgatggaaga tttagttgat ttagacgaaa tcgcttatat tcgttttgca agtgtttatc
    14161 gccagttcaa agatatgtct gttttcttaa aagagttgca agatatcgtg gataaagcaa
    14221 aagaaaataa taaagagaat aataaagaaa acaattaaga aacaaaaagg aggcatttcc
    14281 ttggaaaatg cttggaaaga gcttcaacca aaaaatattt accaaggaag aaaagcggct
    14341 ccgctgagag aagacagcaa gaacgccttg ctgtatcttt atcagccact aatcggcgga
    14401 gaagccttga gtatctattt caccctgtta tcagaaatct ctcaagaatc tggcaaaggt
    14461 cctgaaggga tgcatgcaga tctgcttagt agtctaggct gcggtatccc gcaattttat
    14521 gatgcaagaa aaaagctaga aggaatcggc ttactggatg tttattttaa agaagatagt
    14581 gatctgggac ctcgttttat ttatgagctg ttagaaccac tcattcctaa agcatttttc
    14641 aaagactctg ttctggcttt tcttcttctg gaaaaagttg gtgaacgacg attccagaag
    14701 cttacccaat tctttgaacc taaaaggcat acagcagagg gatatcaaaa aatcacaaaa
    14761 aaatttttag atgtttatcg cttcaatgaa gcagtgtacg ctgctaatca agaaaacatc
    14821 gaatcgctga ataaatcttt tgaagaatct gaatcagtag cattacttga agcagattcg
    14881 acattcgatt ggcagttttt gaatgattgg ctggcaaaaa aacatattgc aagtcttgat
    14941 gatccaatca tcaaacaagt cgacatgtat catcagcttt acggtttcga tgaattgaca
    15001 cttggcgaac tgatcagtca atcttatgac tttagtgaac aaaaagtatc tgaaaaagaa
    15061 ctgcaaaaag ccattttggc aatgcagaga cgtccggtga agcaaacaaa agaaacagtt
    15121 gcggtttctg aaaacagtaa tgaaccctcg tcaaacgaac atttgacaaa cgcagcgctt
    15181 cagctgataa aagaagcaca gagcattcca ccgatgaaat atctggaagc aatcaagtca
    15241 gaaaaaggcg gatatgtttc taaacaagaa aactggttac tgcaagatct tgtttctcaa
    15301 tcaggtcttt ccagtagcgt gatcaatgtt ttgctgaatt atgtactagt gatcaagaat
    15361 aatgctagct taaatgccag ttatgtaaat acagtggcaa atgaatgggc acaaaagaaa
    15421 atcgccacag cagaagaagc gatccagcac attcggcaaa taagtaatca agcaaaacaa
    15481 tcaaaacaaa agaagcaaac aaattatcaa actggcaagc gaaatgttcg acgggaaaag
    15541 cttcctgatt gggtcaacca gccaaaagat gaaaaacaga tcagcagcga gaaaaaagca
    15601 gagattgacc gccgttttaa agaatacctg gcaaaaaagg agggtgatat ctagtggaag
    15661 acgtaggcaa agaattgaag aggatcatca gcaaacgcaa ttatcaagaa aagtatgctg
    15721 aaatgatcgc tgaagtctta aatgatgcgg atgttcaagc ttttctaagc gcacaccaag
    15781 aagaattgac acaagcggat attgaaaaaa gttacgctaa attgtatgaa ttcgtgcaag
    15841 aaaagcgtaa atatcaaaaa aacgatccaa cgatgatcgc tcccggctat gagccgaagc
    15901 tgacgttgaa ttttcattat gtggatgtca cttatgtacc aacggaagca ttgttagcac
    15961 ggcaaagaca agaagaaatc agaagccgag taaaagcaat ggatatgcca aaagatattc
    16021 aatcggcttc ttttgaaaac tttgaacgta cagatggacg aggatatgcg agcttagaag
    16081 ccctagattt tgtagagaaa tatactgctg atccaaaagg gtatcataaa ggactgtatc
    16141 tagtaggaag ttttggtatt ggaaaaacat atttattagg agcaacagcg agagaattag
    16201 cgattgctgg attcaccacg acgctagtcc attttccgac atttgccgtt gagatgaaac
    16261 aggcaatcgg aaaagatcaa gtggcagaaa agttagatgc agtgaaacgt tcacctattt
    16321 tgatgatcga tgacattggt gcagatgcga tgagtagttg gatacgagac gaagtattcg
    16381 gtgttatcct tcaatatcgg atgcaggaac agttaccaac cttcttctca tcgaatttta
    16441 caatggatga attggaacag catttgtccg tcactcaacg tggagatgaa gagccgctaa
    16501 aagcgaaacg gataatggaa cgcatacgtt atttgacaaa agaaatcgaa atgactggtc
    16561 gcaaccgacg caatggttga acagttgaca agtccaactg acatttgacc tagtagtaaa
    16621 agcaggtcag cgaatcatta tatggacaga aatgaccgga gacaatcacc gcaacaagta
    16681 atgcagaaac ttaaacggag gattttttat gagggctatt ttttctagta tatctgtagt
    16741 tggtctattt tttcttttag caggctgcgg tagcgcagat agcaacacag gttctgttga
    16801 ttcgattgat gcgggtcaaa cagtaaaaac aagcagtacg aaaacggaga gacaaagcat
    16861 atttgtcgga acgattgaaa gtatcactca agtagaagca gaagaagaaa cgcttcagtt
    16921 gaaactcgtc gatgtcatgg ccaaagaaga tccagcgaat atgggaacgt cattcagcaa
    16981 tgacggagtg atcttaaatg ctactgctga aaaaataagc gtggacataa atgaattgaa
    17041 aatcggtgac aaagtccaat ttacattagt tgagatgccg atcatgacga tgtctatccc
    17101 accgcaagtt cctggagatt cgattattga tgtttcattg aacaaataac caataacatt
    17161 ttttcaaaaa taagaaaatg ttgttttata gaacgaaaag gtatattttt taaggagata
    17221 tgggaatttt tttattccta gtctccttat tttatttgga aaatgctata attatctaga
    17281 aggagtgaag ctatgaacca tttagattta tctcagcgct tagccgcagt gggtgacttt
    17341 gtccctgaat ccgctcgttt agcagacatt ggatcagatc acgcttactt acccgttgca
    17401 ttgatattaa gaggaaaaat cgaatatgca gttgctggtg aagtcgttca aggtccttat
    17461 gaatcagcac gtaagcaagt caaaaaagat ggattagaag accgtatcac cgtacgcctt
    17521 gcaaatggtc tagatgctat ccagccacaa gatggtatta cagctgtcac gattgcgggt
    17581 atgggaggtt cactgatccg agatatccta gaagctggca agttgaatca acgcttaagc
    17641 ggacaagaac gtttgatcct tcagccaaac atcggagagc gtacattacg atcttggctg
    17701 caagaaaatc agtataaaat ccaatcagaa aaaatacttg aagaaaataa aaaaatatat
    17761 gaaatcattg tagccgaaaa agaacaaaaa ccagttactt acaatgaaat ggagctgatg
    17821 ttcgggccat ttctgcttga agagaaaagt gagatcttca aatcaaaatg gacaagagag
    17881 cttcaacaaa gaaaagctat ccgtgaacag ctcaaacaag cagctaatca agaggcacgt
    17941 caaaaacaaa tcgagcagga aatccaaatg attgaggagg ttttaaagcc atgacaatca
    18001 gcggacgcac tttcatccaa aagtttgaaa gctattgtcc tcagtggcta gctgaagaag
    18061 gagacccagt cggactgcat atcggaacat tggataagcc aatccaacga gtaatgatga
    18121 cattagacgt tcgtccagaa gtggtcgaag aagcaatcaa gaaaaaaatc gatttgctga
    18181 ttgctaaaca tcctccaatc tttcgtcctg taaaacggct ggttactgat cagccacaag
    18241 aaaaaatgta tgcagatttg ttgaaacatg atattgccgt ctatgcagca cacacgaaca
    18301 tggatattat ttgggatgga ctgaatgact ggttttgtga acttctcggt attgaagtgg
    18361 agagttattt agtaaagact catgagattc attataaaaa actggccgtt tatgtacctg
    18421 tggatcatgc acagaaaatg cgtgaagtat tagctgcagc tggtgctggt acacaaggtg
    18481 attataccgg tacaagtttt acctcgattg gacacggtcg atttactccg gaagctggtg
    18541 cacaaccagc gattgggaaa gtagggaaga cagaacaagt tcaagaggca aaagttgagg
    18601 ttattttgcc ggaaactatc gaaaaacaag tgattcaagc catgcggtca gctcatcctt
    18661 atgaggaacc tgcttatgat cttttcgcta tagatgaacc agtcgaaatg tttgggttag
    18721 gacgagtagg tgaattgccg caagaaattt ctatagaggc atttgtagaa caagtaaaag
    18781 aagcgttcca actagatgga ctgagaatag tccagccaaa aaatgcaaaa tcctctgtga
    18841 agcgcattgc gatttgcggt ggaagcggtg aaaaatttta tccgcaagca atcgcccaaa
    18901 gagcagatgt ctatatcacg ggagatatct attatcatac ggctcatgat atgcaaagcg
    18961 ctgggttgat tgcaattgat cccggtcatt atattgaatc gttatgcaag caacgattta
    19021 tcgagaaatt tgaatcttgg aaacaagaag aaaattggga tattaacttt ttcgtttctg
    19081 aaacaaatac aaacccattt caatttaatt aaaaggagag aatactatgt acgaaaatct
    19141 tttgccacgt tttttacgat atgtcaaaac agaaacacgc tccgatgcaa caagcaaaac
    19201 gactccatcg actcagacac aagttgcttt tgcgcaagaa ttgaaaaaag aattagaaga
    19261 gttaggctta gctgatgttt tctataatga agataacgga ttcgttatgg gaacattacc
    19321 aagcaatgtt gatcatgaag tgcgttccat tggatttatt tcccacatgg atacagcaga
    19381 ttttaatgca gtaggagtca atccacaaat cattgaaaac tatgatggcg agtctacgat
    19441 cgtactagat gcggaagaac gctatacatt gaatacaaaa gattttccaa atttgaaaaa
    19501 ttatgaaggc cacacattga tcacaacaga tggcagtaca ttactgggag cagatgacaa
    19561 atcagggatt gcggaaatca tgactgcaat ggagatcttg atcaataatc cttctatccc
    19621 tcacggtacc atcaaagtgg cttttggtcc tgatgaagaa atcggtgtcg gagcggataa
    19681 attcgatgtt gagcaattcg atgtagattt tgcttataca gtcgatggtg gtcctttagg
    19741 tgagttgcag tatgaaacgt ttagtgctgc tcaagcaaat attacgatcc aagggaaaaa
    19801 tgtacatcca gggacggcaa aagatacaat gatcaatgct ctacagttag caatcgattt
    19861 tcataatcag cttccagccg atgaagttcc agaaaaaacg gaaggatacg aaggtttctt
    19921 ccatttgatg gcattggatg ggaatcctga agaagcttct atgagttata tcattcgcga
    19981 tcatgatcgt gaaaaattcg aagcacgcaa agcacaaatc acacatatcc aagaagagtt
    20041 gaatcagcga tttgatcaag aacgaatcaa agtagatatg tatgatcaat attacaacat
    20101 gaaagaaatc attgaaaaag atatgagcat cgtggaatta gcgaaacaag caatgatcga
    20161 gcttggtatc tctccagtga tcgaaccagt tcgtggagga acagatggtt caaaaatctc
    20221 ttacatgggt attccgacgc caaatctatt tgccggagga gaaaatatgc acggacgatt
    20281 cgaatttgtt tctttacaag taatggaaaa agcaacagac gtgattgtaa aaattgcaga
    20341 attgaacaca aagagttaaa atcgaaaaag aacagcttat gacaaggcgt tcgtcccaat
    20401 gatggaatgt catctgtcta aggctaaagg gcaggacaat ggaattggtt gtcccgccct
    20461 cttttttgaa gagtaaatag gaggtaaaga atggaaaagc tgctagtttt ttataacaaa
    20521 agttcaggga aagatgaggg tgagaaatta gcacgctggt ttcaagatta tgtaaaagaa
    20581 aagcagccag aattatatgt agaactagct gaaactggcc catcgattga tgatgcggaa
    20641 ttagtcaaga aagcagaagc catagaagct gatactttag taattatagg tggagacggc
    20701 acgattcatc atatcgtcca agcgttccaa aaaacattat ctcaatacca tgtcggatta
    20761 cttccaggtg gaactgtcaa caaccttgca cgtgttcttg agattcccct caaaaaagaa
    20821 gaagctgctg acgtgatcct ggaaggcaat gtacggaaaa tagactatgg tcaagtcaat
    20881 gatgaagtca ttatctctac actgacaatc ggaatcttag cagatacagc agtttggatc
    20941 agtcaagaag aaaaacagcg ttacggctcg tggatcttta tcaagaagtt ttttaagtta
    21001 ttgctgaaga agaaaaaata tcatttggat ttagaaacag acgaggagca ttggaaagga
    21061 aaatcccaac tgttgacagt cacgatgtct aactccgtgg gcggttttac aaattttgac
    21121 gactcagcga ccctagatga tggaaaattt catgtaacga tcgtaccttc cttagatatt
    21181 ttccgattta ttttttattt gcctagaatc atgagaggca aattctatac gatccccggc
    21241 attacttatt tcacagccaa acgcatgaaa atccaagcgc aggaaaaatc tgtgggcact
    21301 cgaacggatg gagacacgac agatgatctt ccagttgaat tatctgttgt atcaaaagga
    21361 ttaacagttt attcaccaaa gaaagaagaa aaagcttaga aatagtagtt aacagaaagt
    21421 tggaagaaga gtaatggatg tattgttaca caatcctttg tggttttacc ttttcattgt
    21481 gaatttttac ttattttgtc tgatggggta tgataaacat caggctagaa ctagcggctg
    21541 gcgtgttcct gagaataatt tgatatttgt cagcctgtta ggcggtggtt tgggaggatt
    21601 gatctctcga aaagttttcc atcataaaac tcggaaaaag aagtttacta taggattcgc
    21661 tgcaggtgta cttgttgatt tgattctcat ttattttttc cattaaggag caggagatgt
    21721 cgtctaaagg aacgaaaact aaaatcattc tgaatctgct tttgctgatt gtgatttttg
    21781 ccattattta ttatttaatc aatcaatcat ttgcagatat ctttgccgaa ttgctgtcta
    21841 ccagtgttgt tgttttggca gcggctgtta tattagggac agtttaccaa atcgctgaag
    21901 ggcgctcaat caaagaaatt gctgaacctt tcgcgccgaa ttttacaacg aaagatgggt
    21961 tcttgacctc tagctatatt gcgttttacc gaatcgtttc gtttggtaca ggaacgctga
    22021 tctcggagat ttatttttat aacaaaaaag gaatgaagct atcccaggga gcaggggtga
    22081 cagcactcca tatgattatg tacaaactgg cagtgatcac ttatgcatta gctggcttgg
    22141 tcatccaatt ctctcttttt tattctaatg gtccatcaat gatctggttt atcatcgctg
    22201 gtatcctttt gacgatcgtg attgttgcct ttttattggc gatgtccgtc agcttgaatg
    22261 ttcaaatatt ttttgtcagc ttatcgaata agttatttaa aagtgagcgt ttgcggaaat
    22321 gggtggatac atgcaatcag cagatctact ctctaaggga aacggtccaa accattatcc
    22381 aagacaggac tgcgctattg cggatttatc tatggaatat gttaaaactt gtattttggt
    22441 atgtacttcc ttatttgatt ctgattgaaa accatcctaa aatagatttt ctactgacca
    22501 tttcgtttat cagctttgca gtagtgcttt caggggttat tcctacgcct gctggtattg
    22561 gttcctttga atttgtctat ttgttattat tcagaccagt tgttggaaca gttgacgcag
    22621 tttcttcgct tttactgtat cgtttcagtt cctttatctt gccatttctc tatggatttg
    22681 tgtatgtgat tgctgaacgg cgaagagtga tcaaagaaga aataatagaa gtaaaaaaag
    22741 agaataaaga aaatcaaggg taggtctttt ataagatcta ccctttttat caaatcaaag
    22801 aaaaacgtta tggataagat aattttacgt ttgtttggct ctttttttat atacatacaa
    22861 tcaattttgt ttttttaaga tttatataag tcgagaaaaa aagtcaaaaa actatgtgta
    22921 tattttgacc aaaattgacc aaagcgatat aatagttttt gtaaagatca aagaaataag
    22981 tgatcttacc tttaattagg agggtatacc tgtatgaata ttgagaaaat gactacgacg
    23041 cttcaagaag cgattgctga agctcaacag attgcggtca ctcgaaaaca tcaagatatc
    23101 gacattgccc atgtatggaa aatcttttta caatctaatc attttggtcg aaatttctat
    23161 acggatgccg gattggatgt cgaatctttt gaacatgaaa ttgatcgtct actggatgag
    23221 tatccagttg tttctggtgg aaacgtccaa tatggacaaa acctcagcca gaatttcttt
    23281 cgattattga atgaagcaga ccaaatacgt gaaagcttcg gcgacgagtt tctttcaacg
    23341 gaagtcgtta ttttagcgtt gatgaaatta aaaaattatc cgttaactgt ttatttgaat
    23401 aaaaacggat tatctgaaaa agaattgcgt aaaaacatcg aagagatgag aggaggagaa
    23461 agagtgacct cacaaaacca agaagaacaa tacaaagcat tagaaaaata cggcgttgat
    23521 ttagtacagc aagtcaaaag cggaaaaatg gatccaatca tcggtcgtga tgaagaaatc
    23581 cgtgatgtca tccgtattct ttcacgaaaa acaaaaaata atcctgttct gataggagaa
    23641 cccggtgtag ggaaaacggc aatcgttgaa gggcttgctc aacgtatcgt acgcaaagat
    23701 gtgcctgaaa atttaaaaga taaaacgatt ttttcattag atatgggtgc attgatcgct
    23761 ggagcaaaat tccgtggtga atttgaagaa agactaaaag cagtcttgaa agaagtcaaa
    23821 aaaagtgatg gacgtatcct cttgttcatc gatgaaatcc acaatatcgt aggggcagga
    23881 aaaacagaag gaagtatgga tgccggaaat ctgttgaaac caatgcttgc tcgcggtgaa
    23941 ttacatttga tcggtgccac aactttagat gagtaccgtc aatatatgga aaaagacaaa
    24001 gcgttggaac gtcgctttca aaaggtatta gtcaaagaac caacagtgga agatacgatc
    24061 agtattctgc gtggattaaa agaacgtttt gagatccacc atggtgttaa catccatgac
    24121 aacgcattag tagcagcagc tactttatct gaccgttata ttactgatcg gttcttaccg
    24181 gacaaagcaa tcgatttgat cgatgaagcc agtgcaacga ttcgggtaga aatgaactct
    24241 atgccaacgg aattagatca agttactaga cgtttgatgc agttggaaat cgaagaagct
    24301 gcactgaaaa aagaatcaga tgatgcaagt aaaaaacgct tgaaaaattt gcaagaagaa
    24361 ttagctgaac ttcgtgaaga agcaaatgcg atgaagatgc agtgggaaac agaaaaagaa
    24421 gaagtcaatt ctgtttcagc aaaacgtgct gaaatcgata aagcaaaaca cgaattagaa
    24481 gatgcagaaa ataactatga tttagaacgc gctgctgtat tgcgtcatgg tacgatacct
    24541 caattagaaa aagaattgaa ggaattagaa gctaaagcaa aagacagcga gattaagatg
    24601 gttcaagagt ccgttaccga aaatgagatt gctcaagtcg ttggcagatt aacgggtatc
    24661 cccgtaacga aacttgtaga aggtgaacga gaaaaactaa ttaaattgaa tgaaacactt
    24721 cataaacgag tgattggtca agatgaagca gttgatgcgg tcagtgacgc ggtaattcgt
    24781 tcacgggctg gattgcaaga cccaaatcgt cctctaggtt ctttcctttt cctaggacca
    24841 actggtgtcg gaaaaaccga attagcgaaa gcgttagctg agaacttgtt tgactcagaa
    24901 gatcatatgg tacggatcga tatgagtgaa tatatggaaa aacatgctgt gtctcgtcta
    24961 gtcggagctc ctccaggcta tgtaggttac gaagaaggtg gtcaattaac ggaagctgtc
    25021 agacgaaatc cttatacaat cgtcttgttg gatgaaatcg aaaaagcgca tcccgatgtc
    25081 ttcaatatct tgttacaagt cctagatgac ggacgcttga ctgattcaaa agggcgagta
    25141 gtagatttca aaaatacggt tctgatcatg accagcaata tcggttcaca gttactgctg
    25201 gaaggcgtga ctgctgatgg aacgattcca gaagcagtgg ctgaacaagt caacacgcta
    25261 ttacgtggaa acttcaaacc agaattcttg aaccgaatcg acgacacgat tttatttaca
    25321 ccattgagcc tagacaacgt gaaaggaatc gttgacaaaa tggtggcgca attggcacaa
    25381 cgtctggaac atcaagaaat cctgctgaca atcagtgatg aagccaaaac gtggattgct
    25441 gaaaatgctt atgaaccagc atatggtgca agaccattga aacgatttat cactaaggaa
    25501 gtagaaacac cactagctaa agaaatcgtt gcaggacatg ttatgcctaa atcaaaagta
    25561 acgattactt tacttgatgg acaacttcat ttcaaaacag aagagctaga agaaatcgtc
    25621 taatcttcca ttgagactat aaaagaaaaa aggggtctga cacaactagt gtcagacctc
    25681 cttttttcaa ataaacagtg ttccagaagt agtttcttcg aaaaataagc cgaaatttct
    25741 tgaagctgcc cgcttctgtc acaacctctt ttattaggta tttttcactt tttctaattt
    25801 ataactttca aagtaacgct catctttttt actttgagac catgcaaaca atcccattaa
    25861 ggcagcacct atcgtatttg tcagcaagtc gcccatcgta tccatcaatg ctgctcgtcc
    25921 gacaaacagt gtgccatcag aagcagcaaa acgttgaaga ttcatgccta agaactggtc
    25981 gcataagaat tcccagaact cccaaaacac gccgcaaagt ccagcaaacg caaagccgaa
    26041 taagaggaac aaccaaggac tggtttttga tatctcagca tctttcatca agtaaccaat
    26101 caatccatag ccaagagctg tcaggaccat tggactcacc gcatgtaaga ttttgtccca
    26161 aaaagagatg atactgatca aatgcatccc tgtgcctaaa aagacagaga tgaacaagaa
    26221 aaaccagtaa aacaaaacga ttgctgctgg tattttaagt ttgaaaactt tcaaaatgac
    26281 ttgtggcatg aaaatcaatg caatccctaa taaagattcc acgataaatg tgattccttg
    26341 agaagtcgaa tatttttgtg tgataaattc ataaaaatta tagcagacaa cgaatacacc
    26401 aaaagcaagt aaagctattt gcaccttttt gatttcttta atcactgaaa gtccctccat
    26461 ctaaaaatct ttttacttct gaaagcaact caatttctga agaaaccact tttccgttca
    26521 atttgatcaa acctattgta tataagttca ggtagtgaaa ttgattttca gctgtttctt
    26581 gtagtgcagc gatttttttc gggtgggttc cgccttgctg tcgagtatca gtatataagc
    26641 cgatcactgg gatgcctttc gcataagcca caccgatttc cgatgctacc ccggcatcga
    26701 tcgtcagccc atctagtaaa gcaatcatta aatcactagc caacactttt tctgtgtcag
    26761 caagagcaat cattcgactg tctgcataag cagttttgtc attgattgct tcattttctt
    26821 gaggaagata gatcgaaagc tcagaagaga gctctctgat ttctttaact aactgttggt
    26881 tatataacaa atcactttta gcaaacattg gtgcggcaaa ataaattttc atctaaacat
    26941 catccttttt atcaaaaata atcgctaaag taagcttcca tgtttggaat gatacgctct
    27001 agtgacttca gtgtttcttc atctgtttct aaccgttcga ttcttgccaa ataagaagca
    27061 cggcgataat tcatcatcaa gcaagtcaat gtttggatat ccagtcgaac ggattttcct
    27121 ataggttcat ttaagacacg gacttgtccc tgttcatccc atgttaaagc gaagacacca
    27181 ttattccaag gagcgacagg atcttcaatg atgaaatgaa aagcatcagc tgttccaaca
    27241 aacggaaacc gttgaaggaa ttcttttaca tcaacgatcc gagccataaa atatggttcg
    27301 atctgctctt tgatctgact atcttcaatc aaaaatgcta aaggctcatt tttataaata
    27361 tctccatgta cccagtagat catggaaaaa tgtgcggaaa taaagttcca taagccattt
    27421 cgagcttctt gatcaagata gaacatttct tttacgtgaa atatttcttc agctacccag
    27481 tagaacagaa cacctcttgg ttcatggttg ctatcgtagt agacagcggc tgtgcgctca
    27541 tcttcatttt cgaaacgcca atactcttcc caatgaaaat cactgcggaa taaagcacca
    27601 tggttttgtt gggcaaattt gtcataaacg gaaaatacgt ctggatgatc aactggtaat
    27661 cgctcaacca taccacttac tgggatttgt ttaggtagct gtgtatctct gattttaaag
    27721 gaaagtttat cagacatgat ttcccatcct ttacgccgat agtaggggat actgtaagga
    27781 tacaaataag agatccattg cttattcttg cgcatattat ccaatgcaac gataattaga
    27841 tcttgcatca atccatggtt agcatattct ggataagttc caactccagt cacaccaccc
    27901 attttataac gtgcaccgtg aatattgact tcacaaggat agatagcgat ttgagagatc
    27961 agtttatttt cgtggaacca tccgaacact ttcgatactt ccaaaatcgg ttttttggaa
    28021 cggataaaag ctcgtttatt ctcaaacccg ctttcctcga tgtctgcttc agtcacttgg
    28081 aacacatagg aaagcaattc attgaattga tcgatatgtt cttgagtgac cggttttaat
    28141 tcaagatttt ctctaaaatc tttttgatcc attttttcat ctccatttat tagcttgata
    28201 acagtatagc aaaattctac aaaaaaaact tctatagctg actaattaaa aactctttaa
    28261 attactgctt ttcgttggta taatggaaag tgctgattat atgaaaagag gagacgctat
    28321 ggatataaag aaaatgaaag aacgccaaga gcagattcgt aatttttcga tcatcgctca
    28381 tatcgaccat gggaaatcta cattggccga tcgtatttta gaaatgacgc acactgtgac
    28441 gtctcgcgaa atgcaagatc agctgttaga ctcgatggat ttggaacgtg aacgtggaat
    28501 cacgattaaa ttaaatgctg tagaactcaa ttacacagcg aaaaatggag aaacatatat
    28561 ctttcacttg attgacaccc cagggcacgt cgacttcacc tatgaagtct ctcgaagcct
    28621 tgctgcctgt gagggtgccg ttttagtggt cgatgcagca caaggaatcg aagcacaaac
    28681 attggccaat gtttatttag cgttagacaa tgatttggag atcctgccag tcatcaataa
    28741 aatcgaccta cccgcagcag atcccgaacg tgtccgtcaa gaaatcgaag acgtaatcgg
    28801 aatcgatgct agtgaagctg tattagccag tgccaaatca gggattggga ttgaagatat
    28861 tctcgagcag atcgtggaat atgttcctgc accaagtgga gacttagatg caccgctaaa
    28921 agctttgatc tttgactcta tttatgatag ttaccgcggt gttgttctaa atgtccggat
    28981 cacggacggt atggtcaaac ccggagacaa aatcaaactg atgagtaatg gcaaaacatt
    29041 tgatgttaca gaggtcgggg tcttctcgcc aaaagcagtt gctagagact ttttgatggt
    29101 cggtgatgtc ggttatatca cagcaagtat caaaactgtc caagatacac gagtagggga
    29161 tacagtaacg ttagcagaca atcctgccgc tgaagcttta ccaggttacc gaaaaatgaa
    29221 tccgatggtt tattgtggtc tttatccaat tgatacttct cgttacaacg atttgcggga
    29281 agctttagaa aaactgcaat tgaatgatgc tgctttacaa ttcgaaccag aaacctctca
    29341 agcattaggt tttgggttcc gctgtggatt cctcggactt cttcatatgg atgtcgtcca
    29401 agaacgcttg gaacgggaat tcaatctaga attgatcacg actgcaccat ccgttattta
    29461 ccatgttaat aaaacggatg gtacgactgc tacagtagat aacccggcag attttcctga
    29521 acctgtaacg attcaggatg tagaagaacc attcgtcaaa gcacagatca tggtcccaaa
    29581 tgattttgtc ggtgcagtaa tggaactttc tcaacgaaaa cggggagaat tcattacgat
    29641 ggattattta gatgattacc gagtaaatgt cgtctataat attccgcttt ctgaaattgt
    29701 ctttgacttt ttcgacaaat tgaaatcaag cacgaaaggc tatgcctcat tggattatga
    29761 aatgtctggt tatcaaaaaa gcaagttagt gaagatggac atcttgctga atggagaaaa
    29821 agtcgatgca ttaagcttta tcgttcaccg agactttgcc tatgaacgtg gaaaagcaat
    29881 cgttgaaaaa ctgaaaaaac taatcccacg ccaacaattt gaggtgccaa tccaagcagc
    29941 tattggacaa aaaatcgttg cccgttcaga catcaaagcc ttgcgtaaaa acgtcttggc
    30001 taaatgttat ggtggagatg tttctcgtaa acgtaaactc ttagagaaac aaaaagaagg
    30061 gaagaagcgg atgaaacaaa tcggctccgt cgaagttccg caagaagcct tcatggccgt
    30121 tctgaaaatg gacgaggacg aaccgaagaa atagcaaggg tttgtttgaa aacagtgtaa
    30181 aaaataaacc atgtttacat ggatttgcca ccggtttgcc accggtggtt tttttcggtg
    30241 gcaaaaatca agaattcttt ctaagatttt ccatgatatc gaccgtttca tttctcatct
    30301 tatctgttac atgcgagtaa gtatccatcg taattgaaat tctgctgtgg cccaatcgtt
    30361 cagagatttc tttcattttt gcgccatttt cgagaagaag tgtagcatga gtatgtctga
    30421 gagaatggaa gttaaaagag agggagagtg cattcgatat cctcctagta ttccatttca
    30481 ctacacttgg cgtaactagc tcaccgtctt ccttcgtaca tactgcattt gaatcaatgt
    30541 agagctttcc atacttcatt cgattttcta attgttgttt cttatgtttt ttcagaattg
    30601 ctagcaaggt ttgtccaata aaaatcgttc gattggaact gcttgtcttt ggcgtaccat
    30661 atacccatgc gccgtcattc tttaccattt gtttctctac agtaattgtt ccatttgaaa
    30721 aatcgacatc atcccacgtc agaccacaaa cttcgccaac gcgcattccc gtataaaatc
    30781 caatattcaa aggaatatag aaaggatgtc cttcaggagt aatttctagc atatgatcaa
    30841 agtcctcaag agaaatgatt tttagatctt ttttagtcgt tggtcgtgct tcatattttg
    30901 gtatttttac atacagcata ggattttgct tgattaatcc ccaaggataa accgccatat
    30961 tcagcgcatt cttaaggaca gagtgagtaa tagtcattgt tttcttcgag taaccctttt
    31021 taaattcagc attgatgaaa ttttgtaaaa gagcagggga gagatccgta agttttttct
    31081 ttcctaaata accgtttata tgatttttga tggtaaatcg gtagttttca taggtattgt
    31141 attttagatt tagtttaacg tattcctcca tccaaaaatc aaggtattgt tttactcgag
    31201 tatccgtacc taaaaagtat tgtcctgttt cgtcaatatc tgataaaact tttcgtaaag
    31261 cagcttcggc ctctggtcgg gtgtctccgc caactttctc cacttttttt cttgagccat
    31321 catcattgat atc
//
LOCUS       DACDYI010000032        31308 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.032, whole genome shotgun sequence.
ACCESSION   DACDYI010000032 DACDYI010000000
VERSION     DACDYI010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.032"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(45..392)
                     /gene="rbfA"
                     /locus_tag="IWM52_001385"
     CDS             complement(45..392)
                     /gene="rbfA"
                     /locus_tag="IWM52_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAP9973642.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(419..2725)
                     /gene="infB"
                     /locus_tag="IWM52_001386"
     CDS             complement(419..2725)
                     /gene="infB"
                     /locus_tag="IWM52_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAP9973643.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(2738..3049)
                     /locus_tag="IWM52_001387"
     CDS             complement(2738..3049)
                     /locus_tag="IWM52_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAP9973644.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(3046..3339)
                     /locus_tag="IWM52_001388"
     CDS             complement(3046..3339)
                     /locus_tag="IWM52_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAP9973645.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(3361..4536)
                     /gene="nusA"
                     /locus_tag="IWM52_001389"
     CDS             complement(3361..4536)
                     /gene="nusA"
                     /locus_tag="IWM52_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAP9973646.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(4560..5033)
                     /gene="rimP"
                     /locus_tag="IWM52_001390"
     CDS             complement(4560..5033)
                     /gene="rimP"
                     /locus_tag="IWM52_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAP9973647.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(5177..9529)
                     /locus_tag="IWM52_001391"
     CDS             complement(5177..9529)
                     /locus_tag="IWM52_001391"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAP9973648.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            complement(9741..11450)
                     /locus_tag="IWM52_001392"
     CDS             complement(9741..11450)
                     /locus_tag="IWM52_001392"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAP9973649.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            complement(11518..12786)
                     /gene="rseP"
                     /locus_tag="IWM52_001393"
     CDS             complement(11518..12786)
                     /gene="rseP"
                     /locus_tag="IWM52_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAP9973650.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(12947..13747)
                     /locus_tag="IWM52_001394"
     CDS             complement(12947..13747)
                     /locus_tag="IWM52_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAP9973651.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(13744..14556)
                     /locus_tag="IWM52_001395"
     CDS             complement(13744..14556)
                     /locus_tag="IWM52_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAP9973652.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(14751..15308)
                     /gene="frr"
                     /locus_tag="IWM52_001396"
     CDS             complement(14751..15308)
                     /gene="frr"
                     /locus_tag="IWM52_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAP9973653.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(15311..16033)
                     /locus_tag="IWM52_001397"
     CDS             complement(15311..16033)
                     /locus_tag="IWM52_001397"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAP9973654.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAHMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(16169..17050)
                     /locus_tag="IWM52_001398"
     CDS             complement(16169..17050)
                     /locus_tag="IWM52_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAP9973655.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(17149..17931)
                     /gene="rpsB"
                     /locus_tag="IWM52_001399"
     CDS             complement(17149..17931)
                     /gene="rpsB"
                     /locus_tag="IWM52_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAP9973656.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            18290..18769
                     /locus_tag="IWM52_001400"
     CDS             18290..18769
                     /locus_tag="IWM52_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAP9973657.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            18986..20077
                     /gene="phnW"
                     /locus_tag="IWM52_001401"
     CDS             18986..20077
                     /gene="phnW"
                     /locus_tag="IWM52_001401"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAP9973658.1"
                     /translation="MYKLLTPGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSGSFGVESVLSSVIRPEDTVLICSNGAYGQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQEKDLL
                     FIVDAMSSFGGIPIRVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            20070..20855
                     /locus_tag="IWM52_001402"
     CDS             20070..20855
                     /locus_tag="IWM52_001402"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP9973659.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(21201..22892)
                     /locus_tag="IWM52_001403"
     CDS             complement(21201..22892)
                     /locus_tag="IWM52_001403"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAP9973660.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(23314..24261)
                     /gene="arcC"
                     /locus_tag="IWM52_001404"
     CDS             complement(23314..24261)
                     /gene="arcC"
                     /locus_tag="IWM52_001404"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP9973661.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(24376..25395)
                     /gene="argF"
                     /locus_tag="IWM52_001405"
     CDS             complement(24376..25395)
                     /gene="argF"
                     /locus_tag="IWM52_001405"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAP9973662.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(25486..26715)
                     /gene="arcA"
                     /locus_tag="IWM52_001406"
     CDS             complement(25486..26715)
                     /gene="arcA"
                     /locus_tag="IWM52_001406"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAP9973663.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            27176..27877
                     /locus_tag="IWM52_001407"
     CDS             27176..27877
                     /locus_tag="IWM52_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP9973664.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(28449..29465)
                     /gene="tsaD"
                     /locus_tag="IWM52_001408"
     CDS             complement(28449..29465)
                     /gene="tsaD"
                     /locus_tag="IWM52_001408"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAP9973665.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(29462..29926)
                     /gene="rimI"
                     /locus_tag="IWM52_001409"
     CDS             complement(29462..29926)
                     /gene="rimI"
                     /locus_tag="IWM52_001409"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP9973666.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(29933..30475)
                     /gene="rimI"
                     /locus_tag="IWM52_001410"
     CDS             complement(29933..30475)
                     /gene="rimI"
                     /locus_tag="IWM52_001410"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP9973667.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(30459..31283)
                     /gene="tsaB"
                     /locus_tag="IWM52_001411"
     CDS             complement(30459..31283)
                     /gene="tsaB"
                     /locus_tag="IWM52_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAP9973668.1"
                     /translation="MYHCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDKIIVPMFDARRKNVYAGAY
                     RWKENRLEIVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFYEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
BASE COUNT         8491 a         6716 c         5523 g        10578 t
ORIGIN      
        1 aatcccgcaa cccctgattt cacaggattt gcgggatttt ttgattagtc tttgttcaag
       61 tcgcgaagca attcatcgat atggttccca tagacaactg attcatcaag ttcaaagaac
      121 aattcaggtg tcttataaat acttagattg tgccctaact ctcgacgaat caatcctgat
      181 gctttattca atccatcttg tgcttttttc ttatctgatg ctaagtcaga taaaatgctg
      241 taatagatcg ttgcttgctg taagtccccc gtaactttca catctgtgat cgttacgttc
      301 tccacacgag ggtcacggac ctttttgcgc aaaatatcat tgacttcttt taagatttct
      361 tgaccgactc ggcgatcacg gtagttagcc ataatgattg gcctcctttt atcagttttt
      421 agtcattttt gacttcttcc atgataaatc cttcaattac gtcatctact ttgatgtcat
      481 tgaatttttc aatcatcgca ccacattcat aacccatttt gacttctttc acgtcatctt
      541 tgaatcgttt cagacttgca agttggcctt catagattac gattccgtca cggattacac
      601 ggacaccgct atctctacga atatatcctt ctgttacata ggcacctgca atcgtaccaa
      661 ctttagaaac tttgaaggtt tcacgaacga tcatttgacc ggtgattttt tcttcaaatt
      721 cagggtctag catccctttc atcgcagttt cgatttcttc gatggcttta tagatgatac
      781 ggtgaagacg aatatcgact tcttcagtat cagcttgcac ttttgcctgc ggtgtaggac
      841 gaacgttgaa accgatgata atagcgttgc tggctgctgc taaggttaca tcactttcat
      901 tgattgctcc gacagctgaa tggacgattt ttacacggac accttccaca tcaatcttct
      961 tcaatgatgc agccagtgct tctgctgaac cttgtacatc tgctttgatg attacgttaa
     1021 cttctttcaa ttcgccttct ttcaggcttt caaataagtt gtccaaagtc acacggcttg
     1081 tggcagcacg gttttccaat aatgcacgtt ttgcgcgttc ttcacccgct gcacgtgctg
     1141 tcttttcgtc ttcgaagact acgaaacggt cacctgcttg aggtacatca ttcaagccag
     1201 tgatttctac tggcgttgca ggtcctgctt ctttatcgcg acggccaaga tcgtttgtca
     1261 ttacacggac acgtccgtaa gtattaccga caacgattgg gtcaccaacg tgaagtgttc
     1321 cttcttggac aagtaaggtt gacacaggac ctttaccttt atcaagacgt gcttcgatca
     1381 ctgttccgat tgcgcgttgt gttggatctg cttttaaatc ttctacttct gcgactaaca
     1441 ggatcatttc cagcaattct tcgatgtttt ggccgaattt cgctgagatc tcaacaaaaa
     1501 tcgtttctcc accccatgct tcaggaatta attcatactc acttaattct tgcataacat
     1561 gctgaggatt tgcacctggt ttatcaattt tatttacagc gacgatgatt ggaacgcctg
     1621 cagctttggc atggttgatt gcttcaactg tttgtggcat gacgccatca tctgcagcaa
     1681 caactaagat cgtgatatct gtgatacttg ctccacgtgc acgcatactt gtaaaggccg
     1741 catgtcctgg tgtatccaag aaagtgattg gtttaccatc aatatcgatc tggtaggcac
     1801 cgatatgctg tgtgattccg ccggcttcgc cgcttgttac acgtgaatgg cgcaatgtat
     1861 ccaataaggt tgttttcccg tggtctacgt gtcccataat cgttactact ggcggacgtg
     1921 agactaattt atctggatcg acttcttctg cctcgaagaa tttatcgata tcagcaatgt
     1981 caacttggac tttttcttgt ggttccatgc catagtccgt cgctaacagt tcaatcgtat
     2041 ctttatccag tgcttggttt tgattgacca tgacaccaag cataaataat tttttaatga
     2101 tttctgctgg ctcacgatga atttttttcg caatatctgc tacgttcata ccttcagtat
     2161 actctaatac atctggcaat tcacggaatt tgcgtggtgg tacagctggt ttagttgatt
     2221 gttgctgttt gcctttttta ccttttttat tataacgatt acggttatta ttattaaatt
     2281 tgccgcgatt gttattgttt ggtttgttgt tattgttagt acggttttga ttgccgcctt
     2341 ggctcgttcc ttgacgattt tggctgctgg tatttcccgt attattttgg tttgttgttg
     2401 gtttttttcc ttgattcact tgaccgctcc cttgaccgcg atcttgataa ctgcggttat
     2461 ttttattttt ttgttgattg ttcgttgttt cttttttacg ttgatcgttc ggctgttgat
     2521 tggctggttt tctgcttgct tgttgtgcag cttgttgttt cgtgcttggg tttttgaacg
     2581 cttgttgcaa tttcttctca tcactgctag taatcgcacc catatggttt ttcacatcga
     2641 tgccaagttg ttgtgctttt tccacgacgt ctttactaga ttttttgagt tcttttgcta
     2701 attcatagat tcttttgttg cccatgcaat caccttccta accttcaatt agctccttgg
     2761 tcttcttcgc aaatccggca tccattattc cgaatacctt gcgaggtttt ccgatcatct
     2821 gcgtgatttc tgcttcagaa aatagctcaa agcaaggaac ttcataatat gaactcttat
     2881 cttttatctt ttttcttgta ttctcacttg catcggttgc cacaaacacc aattttgctt
     2941 tttgtttgcg gatttcagca atggtcagtt cttcacccgt gattagtttt cccgcgcgca
     3001 tcgctagacc gatgagattc atggcttttt gccgattaac tgtattcatt ttccaaataa
     3061 ctctttacgt gctttttggt gagtgacata gtcaagaagt tcttgataaa actcatctgt
     3121 taatgttgtt tcgagtactc gatcgagaat ttttttatcc caagcttgtt gtacttcttc
     3181 cggctcaagt gagacatacg ctcctcgtcc aggcattttt ccagttggat cgatcgatac
     3241 ttccccttct tttgaacggg tgattcggac catctctttt ttaggtttca tttcacctga
     3301 aaccacggat ttgcgtaaag gaattttcct ttgtttcatc tctttgcctc cttactaaag
     3361 ttaagcttct ggtttttctt cgaccgtttc attgttgaat gcaattgttt catattcatc
     3421 gtctgttaaa tctgactgaa cgattgcttc atcatgcagt tcttctgttt ctttttgttg
     3481 ttcttgattt tcataaaatt ccgtcatgtc agattctgat ttgatatcga ttttatggtt
     3541 agtcaattta gcagctaaac gtgcattttg tccgcgttta ccgattgcta gcgataattg
     3601 gtagtctgga acgacaactg tgcaggcctt tggatttgtc tcatcgaaga tcacatccat
     3661 gacttgtgca ggattcaatg catttgcgat gaatacagct ggatcttcat tccattcgac
     3721 gatatccatg ttttcgcctt ttaattcatt gacgattgct tgaactcgtt gtccctttgg
     3781 cccgacacat gtaccgactg catcgatatt tggatcattt gagcgaactg ccacttttga
     3841 acgatcgcca gcttcacggg cgatgctgac aatttcaacg attccatcat agacttctgg
     3901 tacttcttgt tcaaataagc gacgcaatag gtctggatgg ctgcggctaa caaatacttg
     3961 cggaccttta gacgtatttt ctactcggga aacataaact ttgattcgat catgtggttg
     4021 ataaaattca tttggcattt gatcttgttt agaaagaact gcctcaattt taccaagatt
     4081 gacataaata taacgtttat cctggcgttc gacgattcct tgcatgatgt ctttttcata
     4141 agcactgaat tcgttataaa taatcgttct ttctgcttca cgtacacgct gtaaaataac
     4201 ttgtttagcc gtttgcgcag cgatccgtcc aaaatctttt ggtgttactt caaaacggat
     4261 cgtatcaccg atctcatatg ctggattgat taaaagtgca tctttcaatg agacttccaa
     4321 ctgtgaatcc atcacttctt ccgttacttc ttttaccgca tagacatgga tctttccttt
     4381 tttctgatca aattccactt cgacgttttg tgcttggcca tagtgacgtt tgtatgcaga
     4441 aaccaatgct gcttctaaag catcgatcac gatttctttt gagatccctt tttcagcttc
     4501 taaagcgtct aacgcattta acatttcttt actcattttt ttctgattcc tctccgtgat
     4561 taaaattgga tagctaagcg agcttttgca atattttttc ggtcaaaagt caattctttt
     4621 tctcgtgttt ttatgcgtat tttcaatgta agctgttcag catcaaatga ttgaagaaat
     4681 ccttcatatt gtttttcgcc atctactggt tggtataagg agacatggac ataatcacca
     4741 agtgcctttt catagtcgct ttcttttttc aatggccgct ctgctcccgg ggaggatacc
     4801 tccaggaaat aagcttgagg aatcggatct ggatctgctg catcaagttt ttcactcagt
     4861 ttctcactga caaaggcgca ttcttcgata tcgataccgc cttctttgtc aataaatact
     4921 cgtaagaacc agctttttcc ttcttttaca aattcgactt cgacaagttc aaaattttgt
     4981 tcatctaaaa tcggtgtcac catttctgtg actgtttcaa cgacgctact caaaagtttc
     5041 gcctccttaa aaattaagtg cgtaattcca ttaaaaagag tgagcgaaaa atccgctcac
     5101 tcacagttag tatcattacc tataagaact ataccatact ctgcttaaaa ttacaagcag
     5161 acaggaaaac agatgcttat agcatatcga ataacgataa ttgattttca tctggtagat
     5221 ctttcaatac gccattgtcg ttcatatatt cgatcaatgt ttttgaaact ttaccgcggg
     5281 tggcaagatc ttctttggat aaaaatggac cgtcttttct tgcttcaacg atttgtttcg
     5341 ccacgttggt tcccaaactt gggacagctc taaatggtgc gatcaacgta tcaccttcaa
     5401 taacaaagtt aacggcatcc gatttataca aatcaatcat tccaaatttg aatcctcgtt
     5461 ccagcatttc attggctaac tccagcactg tcagttgatt cttttctttt acggaagcat
     5521 ctaatccctt gtccgtgatt tctttcattg cttgtttcac agcatctttt cctttgcaca
     5581 tggagactag atcaaagtcg tctgcacgta cagaaaagta tgcacagtaa tacaaaatag
     5641 ggaaataaac cttgaaataa gcgacacgaa gagccatcaa tacgtaagct gcagcatggg
     5701 ctttcggaaa catatatttg atttttgaac aagaatcgat ataccagtcg ggcacattat
     5761 tttctttcat cgccgataga taggtttctc gcaattcatc agggatcttg ttccattgtc
     5821 ctttacgaac agtttccatg attttaaatg ccatcccgct atctaatcca gcatggatca
     5881 aataaaccat aatatcatca cgacagccga ttacttccgc cagtgtcgca tctcctcgtc
     5941 gaatcaattc ttccgcattt cctaaccata cgtctgtacc atgagatagt ccggaaattt
     6001 gcagtagttc tgcaaaagtt gtaggatgcg tttcttctaa catccctcga acaaaacgag
     6061 tgccgaattc tggaatacct aatgtccctg ttttggaata aatttgttcc tgacttactc
     6121 caagtacttc aggaccggca aatatccgca tcacttccgg gtcatccgta gggatcgttt
     6181 gtgggtcgat ccctgataaa tcttgaagca tccgaataac agttgggtca tcatgcccta
     6241 gaatatctaa tttcaataca ttatcgtgaa tcgagtggaa atcaaaatga gtcgttttcc
     6301 attctgaatt ttgatcgtct gctggatatt ggatcggcgt aaaatcatag acatccatat
     6361 aatcgggaat aacgatgatc ccccctggat gctggccggt tgtccgtttg actccagtag
     6421 cgccttttgc taatctgtcg acttctgcac ttcgaaactg cagattattg tccctttcgt
     6481 atccttttac atagccatat gctgttttgt cggcgactgt accaatcgtt ccggcacggt
     6541 aaacatagtc ttcgccaaac aatactttcg tatagttatg ggcttctgct tgataatcac
     6601 ctgagaagtt caaatcaata tcgggcactt tgtctccatg gaatcctaaa aatgtctcaa
     6661 atggaatatc atgaccgtct ttgtttaagc gtgcaccaca ttttggacat tgtttttctg
     6721 gcatgtcaaa tccagaacca tacgtaccat cttcaaaaaa ctcggaatat tggcactctg
     6781 gacaataata atgaggggct aatggattaa cttcagtgat tcctgtcatc gtcgcaacaa
     6841 agctagagcc aacagagcca cgtgaaccta ctagatagcc atcttcatta cttttatgca
     6901 ctagtttttg ggcgatcaga taaataaccg agaagccatt accattaata gaattcaact
     6961 ctttttctaa tcgtttttga atgatctcag gtaacggatc tccgtacatt tgttttgctt
     7021 ttgtgtaact caattctgat atttcatctt ctgaacctgg aatcttcggt gtataaagtt
     7081 catctttgac tggaatcact tcttcgcaga tatctgcgat tttattcgta ttttctacta
     7141 cgatctcttt cgctgtttct tcccctaaaa agtgaaatgc agttagcatc tcatcggttg
     7201 ttcggaaatg gacatccggc aaactatgac gattcaacgg attcgctccg cccatagaat
     7261 tgatcaaaat ttttcggtaa atggcatctt cttcgttcag ataatgtaca ttacctgttg
     7321 caacaacgat tttatccaaa ctttttccga tttctaccaa attttggata atttcttcca
     7381 aatcatgttc attttttacc aattcttgct cgatcaaagg cgcatagacc gcttttggca
     7441 taacttcaat ataatcatag aattttgctc ggtttcttgc ctcttctacc cctttttgca
     7501 tcatggcttc gaatatttct cccttatcac aagctgagcc gatcaataga ttttcacgca
     7561 tctttttcaa ttgggagcgt ggtattcgtg ggacgcgttc gaagtattcg acattcgaca
     7621 ttgaaatcag tttgaacaaa tctttcagtc ctgcctgatt ttttgctaaa atcgttacat
     7681 ggaaaggtcg agcccttttg taagaatctc cttctccgat atgctcattt agctgatcat
     7741 gataaagcat gttatgattg tccattgcct ctttcacaaa gatccatgct aagtgaccag
     7801 ttgcttcagc atcatagatt gctcggtgat gctgttccaa gctaacacca aactttttag
     7861 ataacacacc taatccgaaa cgtttgaatt gaggatacag atatctagct aattccaacg
     7921 tatcaatgac tggattggcc gcttcaggaa taccatacct tgcgtagcta gtattcaaga
     7981 accccatatc aaaagccgcg ttgtgggcta ccaaaattgt atcttttgaa aattcaagga
     8041 acatccgcaa tacttcttct tccgactttg accctcgaac catttcatcc gtgataccag
     8101 tcagatctac cgttgttcgt gacaatggat gtcctggatc gataaattca tcaaagcttt
     8161 cgatcacatt tcctttatac attttgactg cggctagctc gatgatcgtg tcatatactg
     8221 cagacaatcc tgtcgtttcc acgtcaaata cgacgtatgt ggcttcactc aatgcttcat
     8281 gtgcatcatt ataagcaatt gggacaccat catctacgac attcgcttcc acgccgtata
     8341 agattttcac acctgctttt tttccagcac tatgtgcttc cggaaaggct tgcgcaccac
     8401 catggtctgt aatggcgata gctcggtgtc cccattttcc tgcttgtgcc acaagatcag
     8461 agatgctgtt tgttgcatcc atcgtactca tattgctatg aacatgaagc tctactcgtt
     8521 tttccccttc aggagcataa tctttgcgag gtgtatgttt gacttctatg atatcttgcg
     8581 cattcatgac caaatcacgg acaaacgtat cttcttgaat acttcctcgt acctttaacc
     8641 agctaccgac actgatcgca tcaaatactt gttcatcttt ttcgccgttc gagaattttt
     8701 tgacgataaa tgatgaagta taatctgtga ttttcaaagt caagatcttg cgttttgaac
     8761 gtagttctct gacttctttg tcgaatacgt acccctcaag tgtgactctt cgttcttctt
     8821 caacgatact gatcattggt gtcgttggtt catcggctgg aatatttcgt cctagctgga
     8881 tgggtccttc aagagctggc gattgctctt ttctttcctt tttcttttgt tcgtggacaa
     8941 ctagactttc agctgcttgc ttcataaagg cttctgcctg ttcttgtttg cgttcttcaa
     9001 aaagtttcaa tacacgttcc gcttgctgct catccacttc tggttcgata cgaaattttg
     9061 ggaatccgta actgacgaac aactcttcta ctaatggcaa gtattgttgc ttcaaataag
     9121 aaatagctcc tgtactgtcg attggcaaga tgactttacg atcttttatc acaggtacct
     9181 gcgttttcaa tacttgctgt gctagtggtg tatcgcattg ctgattttcc aaagcttggg
     9241 cccagtaatc ttgcaagagc tgttcatcga atgtctggtc atctgctaag atattcagtt
     9301 tgacttgtgc aatatcttta aaagcgatag tcagatgctg agtcaatgac cgatatagca
     9361 taataggcaa aattttggaa aaatgtagcg taaattccca taaacgcgat ttacgatgga
     9421 ccactacact atccatttca ccagcagtaa gcagtggatg atttttttct gcttcctcta
     9481 gttggatttg ttccaacagt ttttcaaata aatctcgttt ttctgacaaa gatatacgcc
     9541 ctttctgatg agaaacaatg caattgatcg catcttatcc tcttcttttt cagtaccttt
     9601 tagtatactc gttttttctg agttttacat taaaaaattg gaactgcgat tcaaatcgca
     9661 gttccaattt taaaagcgaa tcatcagatt gtcaaaaaca gctgattcag ctatttttat
     9721 tttctgtgtt ttaacgctta ttattcgcct tggttcaaga ggattggtaa agtattcgcc
     9781 aattcttctt tacgtacttc aaccatttcg ccagttttct tgattttcac ttctacgatt
     9841 ccatccactg cttttttacc gacagtgatt ctgattggac agccaatcaa atcagaatcc
     9901 gcgaatttca ctcctgcgcg ttcatttctg tcatcaacca gtacttcata gccagctttt
     9961 gtcatcgctt cttctagttc attcgtcagg cttgtttggt attcgtcttt tagattcatt
    10021 tggacgatat gcagatcaaa tggcgcaatt ccttttggcc aattgattcc ttgttcatct
    10081 gaatgttgtt ctacgattgc tgataataaa cgacttacac cgataccata acagcccata
    10141 atcacaaatt tttctcgtcc attttcgtct agtacagttg ctcccataga ttcactgtaa
    10201 cgagtgccga ttttgaatat atgtccgatt tcgattcctc gagtaaaggc aagaacaccg
    10261 ttgttgtctg gtgaaggatc tccttcttgt acaaaacgaa gatcttcata agaaagcaca
    10321 gtgaaatcgc gttttggatt cacattttta tagtggaatc ctgtttcgtt cgctccagta
    10381 atggcattcg ctaaatcttg gacataacgg tctgcatata cttttacgtc atctgaaaca
    10441 ccgactggtc cgacagaacc aaactcagca cctagatatt tttgtgcttc ttcttctgtt
    10501 gcttcttcta aaaagtcagc agataaaaag tttttcagtt ttacgtcatt gacttcatgg
    10561 tctccacgta ccaaaacaag gacaggttgt tcatccgcaa tgaacaatac agacttgatg
    10621 attttttgag gttcaacaga aaagaaatct gctacttcgt caattgattt tacgttaggt
    10681 gtagctactt tctccaattc aagctgtgtt tcatgcgatt tttttgatac atagtagctt
    10741 gtagccatct ccaagtttgc agcataatca ctttcagtcg aataacagat cgtatcctcc
    10801 ccgatttctg agatcgccat gaattccttt gagtctttcc cgcccattgc gccgccgtct
    10861 ccgatgatcg agcgaaattc taagccacaa cgcttgaata cttcggtata ggctttttcg
    10921 taatctttat atgtctcgtc caaactttct tccgttgcat ggaaagaata tgcatctttc
    10981 atgataaact ctcttccgcg aagcaaaccg aagcgaggtc gtttttcgtc acgatattta
    11041 ggttggattt gataaagatt taaaggtaat ttcttatatg aattgatttc attacgcatc
    11101 aaatcagtaa aagtttcttc gtgagttggt ccaaggatca tttttcgatc attgcggtct
    11161 ttgaaacgat acaagtttgg tccatatgtt tcgtaacgcc cggattcttc ccaaagttct
    11221 gctggcaaga tggcaggcat cagcatttct accgctccga ttttttcaaa ttcttcccgc
    11281 ataatgcgtt tcagtttttc caatacacga tttgctaatg gcagatatga ataaataccc
    11341 gctgatactt ggcggatata tccagctctt agcaagattt gatggcttaa tacttctgca
    11401 tcatttggaa cttcgcgtaa tgttggaatc aacatttttg attgtctcat tctatatact
    11461 cctttatttc ctcaaaatag ttatcgtttg ttattatacc ctatttgtca actttttcta
    11521 gaaaaagaat cgttgaatat cgttccatgt aactagcacc atcaacaaca ttaaaaatcc
    11581 aaagccagct aatgtcaata ttccttcttt ttcttgactt aatggtttcc cgcgaatccc
    11641 ttcaaaaata ttcaagacta atttcccgcc atctaaggca ggaatcggaa gcagattgac
    11701 gattccgaga ttcattgaaa gaagcgccat caaactaata acagttgtta caccttgatt
    11761 tgctgcttct gaagacaatt ggtacatcat gacaggtccg cctaatttat ctaaactgaa
    11821 acctgtaaat agtgatccta aggctttgaa aatttccaat gaaccgctga aagcttgacg
    11881 cgtccctcca atgatcttat ccataaaacc tgtattcatt ggggctttta taccaagttg
    11941 gccaactttt tcgccatttg actctacaga tttcggtgtc acgtcgacag attgcacctg
    12001 accttctcgt tcgatcttaa agtctaatgt tttacctgga tttttcgtaa tgactgttgt
    12061 cagatcattc catgaatgga tttcttttcc gtccacactg actacctcat cattttcttt
    12121 taagcctgct tctgccgcag ctccattagg catgatctct cctacgcgat tagtgttcgt
    12181 cacttgaaca ccaccttgca taaatgccag aatgatgaat aacacgattg ccaaaataaa
    12241 gttattcatg ggtccagcaa agttggtcag catacgctgc cacaatttag ccgattggaa
    12301 ctgtacgtct tttggcgcaa ttctgatttc tgttccgtcg ttttcaataa tcgtcgcatc
    12361 gtgatcaaca gggtaggtta ctacttctgt ttcgtctcca ttcacataac cagtgatagt
    12421 cagctcatct tctaaatcat aacggctcaa ttccatcggg atagcatttg tcaattggat
    12481 tttcttactt aaatttatct tctctacgat gccgtcagaa tttagtaaaa gagaaagagg
    12541 cattcctggt gccatttctg tttcatcatc cccattgcct gccattcgaa cataaccgcc
    12601 aataggtaat aggcgaagcg tatatgttgt cccgtctttt gcttgatgcc cataaatttt
    12661 cggacccata ccgatagcaa attcgcggac gaggattcct gatcgtttcg caaagaagaa
    12721 atgaccaaac tcatgaacaa tcactaatat accaaaaacg atgataaatg tcagaatcgt
    12781 tttcatagct tttttctcct ttacttgttt tttgtgtttt ttataaaaca acgataaaac
    12841 actataattt taccatagta taccaaagac accaatagta tcaatataat gaaaaataaa
    12901 aacctgcgtc ttttatgaaa ttcacaaaga aacgcaggta tacttactaa ataatgccaa
    12961 ataaatgcat gataggaaat acaaacagca aactgtcgaa acggtctaag attccaccat
    13021 ggcctggtaa gatatttccc gagtctttca catcataatg acgtttgatt gcagattcga
    13081 ccaaatcacc aaattgtccg actgctgaaa aaataatcgt catcagaagc atgacaaaca
    13141 taccatgatt gaatagttct tttgctgggt atagcaataa ataaagaaat gcgactacaa
    13201 cagcactacc gataccaccg agcgcacctt caatcgtttt gttgggcgaa atatcaggac
    13261 ttaacttatg ctgaccataa cgtcgaccaa ccatatatgc tccgatatca gttgcccaaa
    13321 caataaatag accaaatatc aaaacagcca agctttctgt tcgtgcattg acaaaattct
    13381 ggaaacctat tcccacatac aagctgacaa gcacaggaaa acctgcttca tcaatcgtat
    13441 acgtattttt agaaataact gatgttccta aaagaatcat aactataaga taaaataaca
    13501 taaatgtact tacattctct ggaagaaaga aaaaccatct ttcttttggc aaaacaagta
    13561 acactgcacc aacggcggat aaaactcctt caaaacttaa gagtgtcaat cctttcatcc
    13621 ggaacagctc gtaaactcct actaccgcta agattgctgc tgtaagctca accgctatgc
    13681 ctccgatcca tataatcgga ataaacaaga ctagcgccac agctgctgtg atcacacgtt
    13741 gtttcatgac tgatcccctt tctcatcttc tgtttttttt acaccgccga atcgacggtc
    13801 tcgattttga taagaggcga ttgcttcttc aagatgcgca ccatcaaaat caggccaaag
    13861 cgcttttgta aaatacagct cactatatgc gatctgccat aacagaaaat tactaattcg
    13921 ctcttctcca cttgtacgga tcatcagctc aggatcttgt aactctttag gcaagaaacc
    13981 tgtcattaaa tgcttagcaa tcaattcatc atcgatttct tctgcgtgta tttctccttt
    14041 tgctacttct tcggcaattt cttggacagc agtcacgatc tctgctctac ttccataatt
    14101 caaagcaaaa ttcaatacca ttccagtatt gttttttgtt tgctcaattg cacgtcttac
    14161 agcatcttgc gtatgttctg gcaatacgtt ttcataaccc atgacatgaa ctttgacatt
    14221 ttctttaatc aattctggta cgaacgtatc aaagaagtcg acaggtagtt gcatgagaaa
    14281 acttacttca tcttttggtc gcttccagtt ttcagtcgaa aaggcataaa gcgtcaacac
    14341 tttcactcca agccttgatg ctttttttgt gactttcttt actgtttcca tgccttcttt
    14401 atggccagca actcgtggca aacgacggtt ttgcgcccaa cgaccattgc cgtccatgat
    14461 gatggcaatg tgctgaggaa ttggtccatc ggtattaaat tgaaattcgc ttttttcttg
    14521 aatatatttg tttttttgcg gaaaaaaacg taacattctt tttcctccta tacttaaaag
    14581 attcccacta tcttaagtat tatagcaata tctaactgtt tttcctatga aatcattgtt
    14641 tttttattta aaaactgaat aattttttct ttttttaatt tttaaataaa agaaatataa
    14701 ataaaaacag aggaagcgga atgatccgcc cctctgtttt tatgatttta ttaaacttcc
    14761 aataattctt tttctttatc agcagcgatc ttatctactt ctttaatgct gttatctgtc
    14821 aatgtttgta cgtctttttc taatccgcgc aaatcatctt cggtaatatc accatttttt
    14881 tgttgttttt tatattcatc gatcgcatca cgacggatgt tacgaatcgc gatttttgca
    14941 ttttctgctt cttttttcac atctttagct aattcttttc tgcgttcttc tgtcagttgc
    15001 gggataacta agcgaatgac atttccatca ttggttggac tgattccgat atcacttgcc
    15061 ataatagctt tttcaacatc ttgaagaatg ctcttgtcaa aaggtgtgat cattaaaaca
    15121 cgtgcttctg gaatttggat tgatgccatt tggttcaatg gtgttggcgc accgtagtag
    15181 ttcacagtaa tacggtccaa aaggctagcg ttcgcacggc ctgcgcggat ttgcccaagt
    15241 tcacgttgaa gattttgtgc tgcttttttc attttttctt ttgcttctgt cataattgca
    15301 tctgccattt ttatttcccc cttacagttg ttccgatatt ttcgcctaag attgcacggc
    15361 gaatatttcc gtgttcattt aaattgaaga caagcagtgg aatatcatta tccatgctta
    15421 gtgaacttgc agttgaatcc atgacttgta atcctttggc aataacttcc agatgagtca
    15481 gttcttcaaa ttttactgca tttgcatcta ctttcggatc agcagaataa acaccgtcta
    15541 cattgttttt agccatcagg ataacatctg cgccgatttc tgccgctcgt aatgctgctg
    15601 ttgtgtctgt cgagaagtac gggttgccgg taccgcctgc aaagataacg atccgtcctt
    15661 tttctaaatg acgttctgct ttacggcgaa tatatggctc tgcgatttga cgcatttcaa
    15721 tcgaagtttg cacgcgtgtc ggtacaccca cgttttccaa cgtatcctgt aaagctaagg
    15781 cattcataac tgtagctaac atacccatgt aatcagcttg agcacgttcc atgcccatat
    15841 gtgctccgat ttgtccgcgc cagatatttc cgccacccac aacgattgcc atttcaacgc
    15901 ccagctcgtg aacttctttg atttcttgga tgatttcttt gattactggt ggtttgattc
    15961 caaatcctgc atccccagct aaagcttctc cactcaattt taagactaca cgctgatatt
    16021 tcggtgtgac catgttcatt cctccaatat tttctattgc cattttacca tacttagatg
    16081 atttcgacac tctaagactg gtttttcttg ttttgcttaa aaaaagggag cgcacaaacc
    16141 gtgcgttccc gtctgttatt caacagaatt attttttcac ttggctcata acttcttcta
    16201 caaagttatc ttcgcgtttt tcgatacctt cgccaacttc gaaacgtacg aatgttttaa
    16261 cagttgcgcc ttttgatgca acgtattttt caacagtcat atcaggatct ttaacgaatg
    16321 gttggtccac caaagcgatt tctgctttga atttcttcaa gcgtccttct accatttttt
    16381 caacgatatt cgctggtttg ccttcgttca atgcttgttc tgtaagaaca gttttttcat
    16441 gttctaattc agcttctgga atttgtgatt cgtttacata acgaggattg atagctgcta
    16501 cgtgcatagc tacgtctctt gcaactgttt cgtcagttgt tccatctaga acagtcaata
    16561 cggcaatacg tccgcccata tgcaagtatc caccaaatgc agcattgtcg tctttttcta
    16621 caacttcaaa gcgacggaag ctgatttttt ctccgataac ttgagttgct tcgatcaagt
    16681 cagattcaat tgtaccttta tctgttttga ttttcattgc agcttccatg tctgctggtt
    16741 tgttttcagc aacaagttct gcaatttctt ttactaaatc ttggaacatt tcgtttttag
    16801 aaacaaagtc tgtttctgag tttacttcaa cgattgctgc agtgttacct ttcacagcaa
    16861 ctgaagccaa tccttcagct gcaatgcggt catttttctt cgcagccttt gccattcctt
    16921 tttcacgtaa aagatcaact gctttttcca tgtcgccttc tacttcaact aatgcttttt
    16981 tcgcatccat cattcctacg ccagtcattt cgcgtagttc ttttaccatt ttagctgtaa
    17041 cgtctgccat gtttaactcc tcctgtgtgt ttttcttttt aaaaaaagct gcttcaaagg
    17101 agagtgaggc ttgtcgccca gcctttgaaa cagctccatc attcaaaatt attctgctga
    17161 agaattgtcg ccttcaacaa catctacgat ttcttcgatt gatgttgctg tgttttcttc
    17221 tgtgaaatct tcttcaacta cttgatcttc accttggttt ccttcgatga aagcatcagc
    17281 catttttgaa gtaatcaatt taacggcacg aattgcatcg tcgtttgaag ggattactac
    17341 gtcgatttca tctggatcgc agtttgtatc aaccatagct acgattggga tatttaattt
    17401 gtgtgcttct tgaacagcaa tacgttcttt acgtgggtca acgatgtaca ttacatctgg
    17461 gattcttggc atatcagcga taccgcctaa gaatttttca agacgttcac gttctttgtt
    17521 caaaccagca acttcttttt taggtagaac ttcaaaagtt ccatcttctt ccattgcatt
    17581 gatttgtttc aaacggctga tacgtttttg gatagtatcc cagtttgtca atgttccgcc
    17641 taaccaacga tggttaacga agtattgtcc tgcacgagtt gcttcttctt tgattgcttc
    17701 ttgtgcttgt ttttttgtac ctacgaataa tgctacgccg ccttcttcag caacgctctt
    17761 catgtaatca taagctgcat ctactaattt aactgttttt tgcaagtcaa tgatgtagat
    17821 tccgtttctt tctgtgaaga tatatttctt catttttgga ttccagcgac gagtttggtg
    17881 accaaagtgt acgccggctt ctagtaattg tttcatagaa attactgcca tgtttgtttc
    17941 ctccgatttg gttttatttt tcctcttctt atcttctacc ttgccggcaa actttttcaa
    18001 gcacctaccg acaatccaat aagaatgtgg atttgctgat gcataaagca ccctcagcta
    18061 ttatacagac aaaagatccg tatcgcaaga gcaaatatac ttttctgctc aacattcaat
    18121 agtttacctg aaagataaaa agaattcaag aaaaacagta gatttctctt tgtttcttta
    18181 ttatcctttt tccataaaat aattttaggt ataaaagacg aatattattg ggcaatcctt
    18241 cttttttttg ttatgataca tgtgaaatct ttcgtaaagg agaaagaaaa tgcgcaaagc
    18301 tcaaagacat ttgctgatta aacaagtcat tgaagaattc ccgatccgta cgcaagaaga
    18361 actattaaac aaacttgggc attatggcgt taccgccacg caggcaacga tttctcgaga
    18421 tatccgtgat ctaaaaatcg tgaaggcacc tgatgaaaat ggcgtttccc gcttcgtatt
    18481 gtttcgcgaa aaaggaactc ctgagactag gactgaggaa gaggatcggc tggttcaaat
    18541 gattgaagac attgtattga aagtcgaacg agtccacttt ttgacgatcg tcaatacctt
    18601 gccagacaat gctcatttat ttgcatctgt tttagatgag atcaaacctc cctctattgt
    18661 cagtacaatt gctggattcg atacgatcat ggtcatctcg gaaaatgagg aggacgcaaa
    18721 acgagtagaa gaatttcttc accatccaac tgaagtatca cttttttaat cagagcacgt
    18781 attcctaata tggaatgcgt gtttttttat tttcaatatt ttccttatta aataaacctt
    18841 tacactcttt acaaaaatac atcatttttt attcaatttt ttcaacacct tgattgaatc
    18901 acaataatca tcatttatat tggatgttaa caagagcagc ttcttgctct ttgtgtattt
    18961 caaaaaagaa atgaaaggaa atcatatgta taaattatta actcctggac cattgacgac
    19021 ttctgaaaca gtgaaaaatg ctatgctgat cgatcattgt acttgggatc aagaatacaa
    19081 agaaattaca caatggataa gaaaaaaatt attggatctt gctcaagtat cggaaggaat
    19141 atacacaacg gtcttaatgc aaggtagcgg ctcctttggg gtagaatcgg tattaagttc
    19201 tgtgatcagg ccagaagata ctgtactgat ttgttcaaac ggcgcatatg gacaacgaat
    19261 gatcaacatg tgcgaatgtc tgaaactgaa ctataccgtt tatgaggtat cggaacatca
    19321 aatcctccaa gctcatgtga ttacacagct gattgaagag aatccctcta tctccgcact
    19381 agcaatggtc catagcgaga ctacttccgg tattttgaat aatttggaag agattgctgc
    19441 tgtcacacaa gaaaaagatc ttttgtttat agtggacgcg atgtcaagct ttggtgggat
    19501 accgattcgt gtagccgaac tagggattga ttttttgatt tccagctcaa acaaatgtat
    19561 tcaaggtgtt ccaggctttt cttttgtact tgctaataaa gcggaactag aaaagcgaaa
    19621 aaatgtgagt cgaagtttgt cacttgatct atatgaccag taccgcgtga tggaaaacga
    19681 gaatggcaaa tggcgtttca cttcccctac ccatacggtt ttggcttttc aacaagcgct
    19741 gaaggaacta gaggcagaag gaggaatcga aactcgatat tcacgttatc tcgccaataa
    19801 tcatgtacta cgacaaaaaa tgacagagat ggggttccag cctttcattc aagaatatca
    19861 ggggccattc atcacctctt ttctttaccc taaggactgg cgatttgatt ttaatcattt
    19921 ttatcagtat ttaaagcaaa acggatttgt gatctatccc gggaaaatca gcgatcatga
    19981 atgttttcgt atcggaaata ttggcgagat ctatccggaa gatattttgg aactgacagc
    20041 aattatcaaa gactataagg agaacgttaa tgattaaaac tgtaattttt gattgggcag
    20101 gaacaactgt ggactttggc tgtatggctc ctgtccacgc ttttagaaat gcttttctag
    20161 aaaaaggaat ccaactgact gacaaagaaa tccgcgaacc gatgggaaaa ttaaaatggg
    20221 atcatatcca acagcttctt tccctccctt ctgtcaaaaa acaatggata gctatttatg
    20281 gacaactacc tcaggaaaaa gaggtggagg aattgtatca acaatttgaa cattatctgt
    20341 ttaaagattt ggtcaagcat tctaccttga aacccgacac attggaaacg atcgaacggt
    20401 tgagaaacgc tgggatcaat attggttcca cgactggatt tactgctcct atgatgacaa
    20461 tcgtagcaga aaccgcaaaa aaagaagggt attcaccaga cttcatggca acttctgaag
    20521 acgtcgaagg gtatggtcga ccgtttcctt atatgatcca taaaaacatg cagtacttca
    20581 aaaacaagtc ggttgatgaa gtaatcaaag tcggagatac ggtctcagat atccaggaag
    20641 ggaaaaatgc cggagtgttg acagtaggtg taattgaagg tagttcgcta atggggattt
    20701 cttatgatga gtaccaaaaa ttagagatgg ggaaaaaaga tcgtttaaaa cagtctgttg
    20761 ctcttagatt ccaacaagct ggcgctgata tttgtgtaaa caatttaaaa gagttaacag
    20821 aatacatttt gtacgctgac aagaatccag aatgactttt atacaaaatc agaggtcgtg
    20881 aaaaagctgt actgcatcaa gtaataagaa cagccaaaca aaaatagctc ctcatatttt
    20941 tcgtttggtt aggcttatta ccgcagatgc aagcttttga acaccgttta ttcgaaaaaa
    21001 aaagggagct gtgacacgac ttttgtcaca gtccccttat ttacaaacaa acagtgttta
    21061 agagacaacc tctccactaa acggtgttca ttcagctgac tctcggtatt tgctcaaaaa
    21121 acgataaaat ttgaaaagct attttctctt ttgtttctct aatactcgga tcagaacgct
    21181 gatttcacat tctctttttc ttacatctta tctggtgcat gaagtcctag caaacgaagg
    21241 tcttctttca ataatacagt cactgcataa actagagcta agcgtgcttc tttttgttca
    21301 tcatcagcta aaatcttcgt atgagcatag tatttgttga atgcttgtgc taatttgatt
    21361 gcatgtttcg caatcactga cggttcgtat ttttccccag cagacaagac cgtttcaggg
    21421 tatttttgaa ctaatttaac cacttcccag ctggtatcgt cattcaatgc atagtctgct
    21481 gtttctgaag ggacaaatcc tgctttttcc aacaggctga ctgcacgagc atgtgtatat
    21541 tgcacataag gtccagtttc tccttcaaat cgaacaactt cttccaggtt aaagtcaaat
    21601 gtattcagtc gatcgttttt caaatcgtgg aaaatgactg ccccaacccc aacttgttta
    21661 gctacagcat ctttgttttc caaatcaggg tttttctcgc tgatttgttc tttagcagat
    21721 tggattgctt catttaatac ttcttctaat aagacgatct ttcctttacg agttgataat
    21781 tttttaccac cttgcgtgat caaaccaaat ggaatatggc gcatatcgtc agaccagtca
    21841 aagcccattt ccttcaagac agctttcaac tgtttaaaat gatagctttg ttcattccct
    21901 actacatata gagattgttt gaagtcgtaa gtacgtttac ggtacaatgc agcagctaag
    21961 tcacgtgtaa tgtatagtgt cgcaccgtct gacttcttaa tcaacgctgg attcaggtca
    22021 taagcagata ggtcaacgat ctctgcacct ttatcctcgt tcagcaaatg tttttcttct
    22081 aaaagtttga cgatttcatc catcttgtca ttgtagaaag cttcaccgtt caacgaatcg
    22141 aaacgtactt ccagcagatc atagatttta ttgaattctt tcatcgattc atcacggaac
    22201 cattgccata attgaatagc ctcttcatcg ccttcttcca gacgtttgaa ccaagcacga
    22261 gcttcttcat ttaattctgg ttctgtttca gctacttcat ggaattggac atataaacga
    22321 agcaattcat tgattggttc tgctttaaca gcttcttctg taccccattt tttgtaagcg
    22381 acgatcagtt ttccaaactg tgttccccag tcacctaaat ggttgatgcg gattggctga
    22441 taaccgattt tttccataat gaaaccgatc gaattaccga taactgtcga acgcaaatgt
    22501 cccattgaga ttggtttggc aatattagga gaagacatgt cgattggtac tgttccttga
    22561 ttcccgatat tgctatcccc ataatgttct ttttctttca ctactgtttg caaaactttt
    22621 ttgctgataa gttctttatt catgaaaaag ttcaagtaag gtccgacaac ttcgattttt
    22681 tcaaagttgg cagaatcgat tttttctgct aaatctgcag cgatttgctg tggcgctttt
    22741 cggtaaactt ttgctaatga aaaggctgga aatgcaacat ctccgtgttc agctgatttt
    22801 ggattttcta gtaattgttc gacttgttcc agtgttaaat catctttgac agcgtcatag
    22861 acagcttttg cgacaagatc tttattattc atctttattc ctcctaaagt gctcccttga
    22921 ttgttaaaca aaaaaaatct ctagcgacgg catacttagt atgccatcac tagagacgag
    22981 atttcccgcg gtaccactcc aattgtccta aaaaaggacc agctccatct gatatcggta
    23041 gacgaccgtc tgtttaaaac agaattctcc aagtgcgctt cgttgtatct tcttcattcg
    23101 gcttccacca cttccgaatt cgctagccaa gaatgataca agtactctct tgttcaacaa
    23161 atcttagtct tattaaactt tcttgaatta tatcagaaaa aaaaacgaaa ggctagtcgt
    23221 ttctgataaa aaaacaatcc agatggccta aaggagaaga aataccacct ggatagctat
    23281 aaatcatttc gtaaatacga aagacttaac aaattaatca gctacgataa tcgttccttc
    23341 ttcagaagct aaaagattct cgatattttc taaagaagtg atgattgcct tagcgtttgg
    23401 ttttgcttca acaaatgcaa tagctgcctc tactttcgga agcatgcttc ctggagcaaa
    23461 ttgcttttca tcaatgtatt gtttcatttc tgaaacagta acagtttcta atttcttttg
    23521 atctggtttt tggtaattga catacacatt gtctactcct gtcaatacga tcagcaaatc
    23581 cgcatcgatg atttcggcta atttttctga agcaaaatct ttatcgatta cggcttctcg
    23641 tccttctaat ccagtagctg tttctacaac aggaatccca ccgccaccaa cagagactgt
    23701 gataactcct tgatcgacca gcgtttcgat gacacgtgct tctttaatcg agatgggttt
    23761 aggggaagcg actacttttc tccatccacg tccagcatct tctttgaatg tcacagtgga
    23821 gtcagcattc atttgttctt tggcttcttc ttccgtgtag aatggaccga ctggtttact
    23881 tggatttttg aatgaaggat cattttcatc cacaataact tgagtgacta aagaaaccac
    23941 atctttatcg atcccttttt ccttcagtac ttcgcccatg gcattttgta accagtaacc
    24001 gatagatcct tctgtcattg caacacatgt atctaaaggc attgccggtg ttttctcaga
    24061 atcagcagct tgctgctgaa tcaataggtt accgacttgc ggaccatttc cgtgggagat
    24121 gatcaattca tctccttgtt cgataaattt cactaaatac tttgctgttt ccattaacgc
    24181 ttcttgttgg gcttttgcac ttgcatccgt agacaagatt gcattacccc ctaaagcgac
    24241 aactactttt cgtttggcca tttcattcgt cctttcttca gttctaccga attttctaga
    24301 taacaaaacg agccagcgtt ttgtgatttt ccctcaatgc tgactcgttt tttcgtgaga
    24361 tgaatgtggt tatgcttaaa cgcgagggat aaacaagtta cctaatgttg ctgccatgat
    24421 cgctttgatt gagtgcatac gattttctgc ttggtcaaat tgacgagcat atttgctgcg
    24481 gaagacttca tctgttactt ccatttcagt gatgccaaag cgttctttca tttgttcacc
    24541 gtaaacagtg tttgtatcat ggaaagctgg caagcagtgc aagaagatca aacgatctgt
    24601 gttgtgtgtt ttttctacca tttccatatt gatttggtaa ggtttcaaca atttgatacg
    24661 ttcttcgaat ttatcttctt cacccattga tacccataca tcagaataaa ggacgtctgc
    24721 tccgtctaca cctttatcaa catcgtcggt aatcatcaat tgtgcaccgg atttttcagc
    24781 aaagccttct gccattttga cgatttcttc ttcaggttgt aattcttttg gtgcaacgat
    24841 acgcatattc gctcctaaaa ttgcacctgt gactaataaa gagttagcca tattgtttcg
    24901 accatctccg cagtaagcaa cagtgatacc ttctactgtt ccaaagtttt cttggatagt
    24961 taaaaagtca gcgatcattt gtgttggatg ccattcatca gtcaagccat tccaaactgg
    25021 aacaccagag aattctgcta gttcctctac cattttttga ctgaatccac ggaattcgat
    25081 tccatcaaac atacgaccta gtactttagc tgtatcttct gtggattctt ttttacctaa
    25141 ttggatatca tttgcaccta gatattcagg atgtgcacct agatcgatcg ctgcagtagt
    25201 aaatgcagaa cgagtacgtg tagatgtttt ttcaaataaa agcgcaatat tttttccttc
    25261 tagatagtga tgtgggatac cacgtttttt caagtctttc aagtgttctg aaaaatcgat
    25321 caaatattgt aattcttctt ttgtaaagtc tttttctgct aataagcttc ttccttggaa
    25381 tactgattct ttcatattaa acattctcct ttagattgtt tttatttaag attaaagagg
    25441 aaatcccgac ggcagctgac agtaagaccg ccgtcaggaa acaaattact tcaagtcttc
    25501 acggacaagt ggttggctca tgcagcgtgg accaccacgg cctcgtgaca attcacttga
    25561 attgatttct aacactttga tgccatagct acgaagcaat tcgttcgata cataatttcg
    25621 gttataagtg actacaacac ctggagcgat tgccaatgtg tttgatccat cattccattg
    25681 ttctcgaggt gcaacgatct catctccatt accagttggg atcagtacaa gatcatctaa
    25741 tccaagagca gctttcatcg tttcgtgtaa gttatctgaa tgagtaatct tgatatcatc
    25801 accatctggt tcgattgtaa agacatccat cttaccgtct ttactttgaa ttgctggatg
    25861 gatagtgaat ttatcgtgat ctaccattgt aaatacggta tccaaatgca tcattgcacg
    25921 gttgtttgga attttgattg ctaacacttt ttcaaatcct gaattttttg cgaataggtg
    25981 gcgtgctaat ttttctaaag ctttcgcatt agttcgttga gaaatcccaa cagctactac
    26041 tttatcgctt aagatcaatt cgtctccgcc ttcgatatga tctggatttt cacgatccaa
    26101 ccatacttcg attcctttat ttgcaaatct tggatgatgt ttcaaaatat attctgtaaa
    26161 catcgattca cgacgacgtg cttcaaaagt catcttattg actgtcatcc catttcccat
    26221 tgatgcggaa gggtcgcgtg taaagtacaa gtttggcatt gggtccatgt aaaatggata
    26281 gtcatcgtct gctgacagat cgtatagact gctagaacgt acttcaatat cttttgtacg
    26341 aaccccagcc atgattttat ctaccatttc ttgcgtttcc attgatagaa ggaactcatg
    26401 aagaccctct cttacagcat tcgatgcaat atgagattca tccaacattt tattcaagaa
    26461 ttgttctttg acattgcctg cgtcaatcgc ttctgccgct aatttttcca agtaaagagt
    26521 ctcgacgcct tcattttgca atgtctttgc aaagttgtca tgttcttctt gagcaatcgg
    26581 aagatatgga atatcatcga acaacagacg atccataata tctggtgtca aattttccac
    26641 ttcttgtcca ggccttttca gtaaaactgt ctttaatttc cctatttcag agaaaacgtg
    26701 aataggttta tccatgttgt gttgcctcct tctttttgat tacatgtata ggatatcctc
    26761 cttttgtaat cactttcata attttgtctc attcaaaatg agacattcct cacattaata
    26821 ttcgataatt aaataattaa acgtttttct atttttaagc gtaatttatt cattttttat
    26881 tctgtataca ctttcaaaaa aatatttatt cattgaaaaa aattttttga ataaattaaa
    26941 aaaataaatt atattagcgt tgttagcgtt tactatatgt attattgttg ttttttatac
    27001 acaaatcatt cgcttcaaaa caaattattc aataccattg tatatttttt acataataac
    27061 aaattatatt tgtaaagtat taaaacttgt ttctaatgtt ggtatgttaa aactttacaa
    27121 attctttaaa aaaagtgaca ataaatatag tgaacaataa ggagaggact ttttaatgcg
    27181 tgttaataaa atcgattttc acttttcaaa actcagatat caacctgatt ttgaacattt
    27241 ctcggatgaa gagttccaac agatcaagga acatactgtt cttcgttctt ataaaaaagg
    27301 acagatcctt ttcgacgatg gtgatcccag acaacggttg tatatcttga ccaaaggact
    27361 cgttcgttta gaacgttatg atgaaagtgc aacttattac tactatgact atgtgaatac
    27421 aaataacctt tttcctatgt caggaatgtt tggagaagag acctacactt atacagctca
    27481 agcgatgact gatattgaaa cattctacct tcctgtttcc tactatgaaa aattagcacg
    27541 aaaaaatccc gaacagttga tctatatgat acaaaaaatt tctaaagtag ttgaaataca
    27601 tgaattgcgt atccaaatcg gattgacctc tagcgccttt gaccgggtaa aacaaaattt
    27661 attcattttg aaagaagaat tgggcgtacc aacaaaagat ggtcatatca ccattcctta
    27721 tccgatcacc ttaaaagaat tagcagtgaa tagcggcacg acacgtgaaa ctgctggaca
    27781 agtaatcaaa caactacgtg attccggact gttagattat gacaaaaagc gttttacttt
    27841 ctgtaagaca gctactactc cagctatgag agaataacgc gaacaaaaat aagcagtgac
    27901 cctgatattt atcagggtca ctgcttattt tcctccctaa aacttctgaa agatccggca
    27961 gttttagatc actgggcaag actcaaagat tttctatgtg tgtttcagtt atgtaatatt
    28021 cactgtttgc aaaaaatact gatagattaa ctagaggaag cgtcttgtcc tcatccgacc
    28081 tagattattc gatattcctc gacaataaac gattaatcag tgagggcaat cagaggaacg
    28141 gataatgatg aataagctgt aaaaaatagg gctttgacaa aagcgtgtca cagcccttat
    28201 ttcgattaaa cggtgtgtac ctgtcaactc ttcggtcaca agtcataatg ttcataaaca
    28261 tgaggagctg ttttttgaaa ttgttccttg tgaatcagag ttaaacgaca ggcttacgaa
    28321 ctcttgatta aacggcgttc aaaagcttgc atacgcggta ataagaacag ccaggtcaaa
    28381 aacatgagaa gccgtttttc ctgggctgtt cttattactt gatgcagggc agatttttca
    28441 caacctcttt attcagataa aaccaagctt ggatcagcat tcaactgata gctagcaaaa
    28501 tttctttttt gcatttccac atgtgctgct gctccaatca ttgcagcatt atctccacaa
    28561 agtcgaagag ggggaatcac tagttctacc tcagggagtt tagttgccaa agcatcctgc
    28621 aatccttcac gcaacccttg attggccgct acacctccag ctacaatcag ttgcttgact
    28681 ggataatttt gacaagctcg aagtgtttta gtaatcaata catcgatcac acttgcttga
    28741 aagctagctg ctaaatcatt tttgtctaga tcttctccac gctgttgtgc attatgcacc
    28801 aaattgataa atgcactttt taatccgcta aaactgaaat cataattatc ttcatggatc
    28861 atcgctcgtg gaaaatggta attatctttt ccctgatgag ctagctgatc gatttctttt
    28921 ccgcttgggt aagaaagacc taatactctt ccaactttgt cataagcttc tcctgctgcg
    28981 tcatctcttg tctccccaat gatttcatat gatccatctt cttgcatata gactaattct
    29041 gtatgtcctc cgctaacaag aagtgccatc aatggaaact ggaatggttt aaccagacgg
    29101 gctgcataga tatgccctgc catatggttt acaggaataa gcggcaattg gtgtgcccaa
    29161 gcaaaagcct tagctgcaga aataccaatc agcaacgagc cgacaagtcc tggtccataa
    29221 gtgacagcga ctgccgataa atcttctgcc gacacaccag cttctaccag tgcatcttcc
    29281 aagcataagg tgatttgttc tacgtgatgg cggctagcta cttcaggaac tacgccacca
    29341 aaacgtttat gactgtttat ttgagaagca acgatgttag ataagatctc tgttccattt
    29401 ctaacgaccg ctacactcgt ttcatcacaa cttgattcaa tcgccagaat caattcttca
    29461 ttcatttcat cgtctccgtt tttaactttc tgctcataat gatcgcatct tccgtcggat
    29521 tgcgataata tgatttccgt ttacctaata tacgaaactt ttctgattca taaagttttc
    29581 ttgctgcttc attagataga cgtacttcta aaaaaacatg cagcgaatct tttatttgtt
    29641 ccgtaatcag taattgtaac aaatgccgtg catgcccttt tccttgttct gaaactgcaa
    29701 cagcaatatt tgtgatctcg acttcatcta ttactttcga gtatccaata aagccacaga
    29761 tctggttatc ttcaactaaa agcagataat cagagtgagg ctgttggatg tctgccaaaa
    29821 actgcatttt cgtccaaggt gaaccatgtg gataagcatt ttcgctaatc atccatacgc
    29881 attctgccaa gtacgcttca tcaaaatctt tttttctgca aatcaactaa tctcactcat
    29941 tttctaaaag ttttatcata tccaacgcat cttcttttgt ttcagtgtaa tatctttttt
    30001 tgatcgttgt tgcttgaaaa cctaacttcc gataaagtct ttgtgcatct tgattactca
    30061 tacgcacttc taatgacatc gtctcacagc gattcattat agcaaagttc tcgatttcgt
    30121 caataagcag cgaaccgatt cgtttacgct gatatgccgg tcgaacggca atattcgtga
    30181 tatggcaatc taaaccgaac gctcttgaac cgataaaacc aatcattctt ccattatcaa
    30241 agatacctag atatagatga ggaagcggtg acttcaattc tgatataaag gcactttttg
    30301 tccatggcaa ttcgccatta tagacttctc gttcgacatc cagcagctct ttgatatctt
    30361 ctgtagtcaa ttctcttagt acgtaacgac gatcgatttg ttcgattgtt ttttctgtat
    30421 aaatcatttt atcttgaaaa aagcttctgg catacgctct aaattttttc aacataactt
    30481 tcgtctccag gtgtgtgcgt ctccaaccat ttttcttctg cttctacccg ttttaaatag
    30541 cgaggaagaa aagcttgttt ctctgcaact ggttcagcta aagcacctaa ttgagccaca
    30601 gttacaccac ttggaatatc ccatgctgga actgtattaa tcatcgcact aggcaatatt
    30661 tcctttattt catagaagaa tttgtgacaa tcactcccga cgaaataaat agcttcattc
    30721 agcttttgta gttgtgtgaa caaatcagaa gcagaaatat gtacatcttc aagtacaatt
    30781 tctaatctat tttctttcca gcggtatgcc ccagcgtata cattttttct tcgagcatca
    30841 aacatgggca caataatttt atccacaccg atgcaattag ccgcgatggt tttcagacta
    30901 gatactccta ctagctcttt atctaatgta taagcaatcg tttttgctgt cgttacacct
    30961 atacgaagcc ctgtataaga accgggacca tcagaaaccg cgattcggtc aatgtctttt
    31021 ggacttattt gaagatctgc aaacaattgg tcgattgcag gcatcagtgt tacactgtgg
    31081 ttgcgtttga tatttgtttg tatttgtcca agaatttgtt cgtcttctac cacaccgatc
    31141 gccaaagttt ggtttgcagt atctattcct aatgtaatca tatatttgat cctttctgct
    31201 atgacattct cgccttccat tgtagcatac ttttttattc tgaggatgaa aataccaaag
    31261 gcaaaactag tacaatgata catgattatc tgctggcggg caaatttt
//
LOCUS       DACDYI010000033        30661 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.033, whole genome shotgun sequence.
ACCESSION   DACDYI010000033 DACDYI010000000
VERSION     DACDYI010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.033"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(434..1918)
                     /gene="guaB"
                     /locus_tag="IWM52_001412"
     CDS             complement(434..1918)
                     /gene="guaB"
                     /locus_tag="IWM52_001412"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAP9973669.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(2423..3118)
                     /locus_tag="IWM52_001413"
     CDS             complement(2423..3118)
                     /locus_tag="IWM52_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAP9973670.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(3208..4308)
                     /gene="ychF"
                     /locus_tag="IWM52_001414"
     CDS             complement(3208..4308)
                     /gene="ychF"
                     /locus_tag="IWM52_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAP9973671.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(4317..4508)
                     /locus_tag="IWM52_001415"
     CDS             complement(4317..4508)
                     /locus_tag="IWM52_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAP9973672.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(4529..5419)
                     /locus_tag="IWM52_001416"
     CDS             complement(4529..5419)
                     /locus_tag="IWM52_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAP9973673.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(5406..6173)
                     /locus_tag="IWM52_001417"
     CDS             complement(5406..6173)
                     /locus_tag="IWM52_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9973674.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(6185..6898)
                     /gene="rsmG"
                     /locus_tag="IWM52_001418"
     CDS             complement(6185..6898)
                     /gene="rsmG"
                     /locus_tag="IWM52_001418"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAP9973675.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(7130..9031)
                     /gene="mnmG"
                     /locus_tag="IWM52_001419"
     CDS             complement(7130..9031)
                     /gene="mnmG"
                     /locus_tag="IWM52_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAP9973676.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(9048..10445)
                     /gene="mnmE"
                     /locus_tag="IWM52_001420"
     CDS             complement(9048..10445)
                     /gene="mnmE"
                     /locus_tag="IWM52_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAP9973677.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            10789..11127
                     /locus_tag="IWM52_001421"
     CDS             10789..11127
                     /locus_tag="IWM52_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973678.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(11295..12053)
                     /locus_tag="IWM52_001422"
     CDS             complement(11295..12053)
                     /locus_tag="IWM52_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP9973679.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(12071..12889)
                     /locus_tag="IWM52_001423"
     CDS             complement(12071..12889)
                     /locus_tag="IWM52_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP9973680.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(12886..13242)
                     /gene="rnpA"
                     /locus_tag="IWM52_001424"
     CDS             complement(12886..13242)
                     /gene="rnpA"
                     /locus_tag="IWM52_001424"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAP9973681.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(13360..13494)
                     /gene="rpmH"
                     /locus_tag="IWM52_001425"
     CDS             complement(13360..13494)
                     /gene="rpmH"
                     /locus_tag="IWM52_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAP9973682.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            14017..15351
                     /gene="dnaA"
                     /locus_tag="IWM52_001426"
     CDS             14017..15351
                     /gene="dnaA"
                     /locus_tag="IWM52_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAP9973683.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            15549..16679
                     /gene="dnaN"
                     /locus_tag="IWM52_001427"
     CDS             15549..16679
                     /gene="dnaN"
                     /locus_tag="IWM52_001427"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAP9973684.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            16908..17150
                     /gene="yaaA"
                     /locus_tag="IWM52_001428"
     CDS             16908..17150
                     /gene="yaaA"
                     /locus_tag="IWM52_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAP9973685.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            17137..18261
                     /gene="recF"
                     /locus_tag="IWM52_001429"
     CDS             17137..18261
                     /gene="recF"
                     /locus_tag="IWM52_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAP9973686.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            18258..20204
                     /gene="gyrB"
                     /locus_tag="IWM52_001430"
     CDS             18258..20204
                     /gene="gyrB"
                     /locus_tag="IWM52_001430"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAP9973687.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            20224..22695
                     /gene="gyrA"
                     /locus_tag="IWM52_001431"
     CDS             20224..22695
                     /gene="gyrA"
                     /locus_tag="IWM52_001431"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAP9973688.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            22931..23230
                     /locus_tag="IWM52_001432"
     CDS             22931..23230
                     /locus_tag="IWM52_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAP9973689.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            23280..23792
                     /gene="ssb"
                     /locus_tag="IWM52_001433"
     CDS             23280..23792
                     /gene="ssb"
                     /locus_tag="IWM52_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9973690.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            23818..24054
                     /gene="rpsR"
                     /locus_tag="IWM52_001434"
     CDS             23818..24054
                     /gene="rpsR"
                     /locus_tag="IWM52_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAP9973691.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            24197..26170
                     /locus_tag="IWM52_001435"
     CDS             24197..26170
                     /locus_tag="IWM52_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAP9973692.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            26176..26628
                     /gene="rplI"
                     /locus_tag="IWM52_001436"
     CDS             26176..26628
                     /gene="rplI"
                     /locus_tag="IWM52_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAP9973693.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            26902..28269
                     /gene="dnaB"
                     /locus_tag="IWM52_001437"
     CDS             26902..28269
                     /gene="dnaB"
                     /locus_tag="IWM52_001437"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAP9973694.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            29049..30341
                     /locus_tag="IWM52_001438"
     CDS             29049..30341
                     /locus_tag="IWM52_001438"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAP9973695.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         9258 a         6057 c         6055 g         9291 t
ORIGIN      
        1 tttatcgaga ggttgtgaaa tcagcgaatt aacctgagta ttagagaaaa aaatggaaaa
       61 atagtccctc atattttacc gtttgttgaa ctaataccga gggtcagctg attgaacacc
      121 gtttagtgga gaggtcgtaa gcctaccccc cagctcctag ttacaaggac aaattcaaaa
      181 aacagctctt catattttat gaatgttgta cttgtgattc ggaggtggca agggaacact
      241 gtttattcaa aaagaggctg ggacagaagc gtttaactcc aagaaataag aagaaattca
      301 cgaaaattgc ttttcaaatt tttgtgaatt tcttcttatt tccgaaggag ttgcttctac
      361 ttccgccgtt tatacgtgtt tagagcgtga gacaaaagtg ttctttactt ttgtcccacg
      421 ctctttaatg tttttaagat tcaactgaat agtttggtgc ttcttttgtg atttgtacat
      481 catgagggtg tgattctttt aatccactgc cgctcatttg gataaattgc gcttcatcac
      541 gtaattgctg tagattggct gccccaacat aacccattcc tgaacgcaaa ccgccgatca
      601 tttggaaaat aatatctgca acacttcctt tataagcaac ccgaccttcg attccttctg
      661 gtactaattt atttgcttca ttgacactac cttggaagta acggtctttt gaaccttttt
      721 ccatcgcgcc taatgagccc atacctcggt atgttttgaa tcgacgtcct tggaagattt
      781 cgaattcacc tggtgattca tctgtccctg ccaacatact tcctagcatc actgcatgtc
      841 cgccggcagc taatgctttg acgatgtcac cagaatattt gatccctccg tctgcaataa
      901 tcgctttgcc atattgacga gcaactgatg ctgcatcata aatagctgtg agctgaggta
      961 cacctacacc ggctaccaca cgtgtcgtac aaatcgatcc tggtccgata ccaactttta
     1021 cgacatctac acctgcatca tataaagctt ttgtcgcttc agccgtcgca acgtttcctg
     1081 caatcaatgt tgcatctgca aaagttgaac ggatttcttg aatttttcgg atcacacctg
     1141 cactatgtcc atgagctgta tcgatgatga ttgcatctgc ccctgcttct aataaagcat
     1201 tcgcacgctc aaatgtatcg cttgttacac caacggctgc tgctacaagc aatcgaccat
     1261 gttcatcttt tgctgcattt ggaaattcga tcactttctc gatgtctttg atcgtgatca
     1321 atccgcttaa acgaccttcg ttatctacga ttggtaattt ttcgatttta tgtttttgca
     1381 aaatcttttc tgcatctttt agagaggtac ctactggggc agtcactaag ttgtcttttg
     1441 tcatcacatc agcaatcggc atgtgataat ctgttacaaa acgcatgtcg cggtttgtaa
     1501 ttatccctac caattttctg ttttcaagtg tttcaacgat tggcacacca ctgatacgat
     1561 atttgctcat caattcttct gcgtctgcta caagatgctg tggcgttaag aagaacggat
     1621 caatgattac gccactttca gaacgtttga cttttcttac ttcatccgct tgctggctga
     1681 tcgtcatgtt tttatggatc acacctagtc cgccttgtct tgccatagca atggccatct
     1741 tgctgtcggt aacagtatcc atacttgcac tgatgattgg aatattcaac ttaatatttt
     1801 tagctaattg tacgctcata tccacttcgt ttggtaacac gtgactttct gctggtatca
     1861 ataagacatc atcaaaagta aatccttttt tcgtgaactt tgtttcccag ttggacattt
     1921 ttagtaccac tcctctaatt tagtttatct attaaaataa ttgatttttt catttaagtc
     1981 aatgatctac ttggaatcaa gttagctttc tcatagaata ccaatataaa gcgtttacat
     2041 ctgtgtgtat taaaagagat tgtaaaaaag ctgtcctgca tcaagtaata agaacagcca
     2101 atcaaaaaca gcttctcatg tttttgaatt ggttgggttt attaccgcag atgcaaactt
     2161 ttgaacaccg tttagtggag aggctgtgaa aaagctgctt agtttcgagt agtaagaaaa
     2221 attttgtgaa aatggctttc cacatttttg cataaattgg acttaatacc gagaaactgg
     2281 cttttgaaca ccgtttatcg taaaaaggga ctgtgacaag acttttgtca cagtccctta
     2341 ctattattta actatggata aacaaaatga ggttctttac tacccctaga tatctagcaa
     2401 aatagctgat ctttttgcta gtttatcttc ttgggacgga taaacttcca ataatcccgt
     2461 attttttctt caagtagtaa cggattgcta aagcagttgc cccgataatg ataactacta
     2521 ctggatctaa tactggattg ataactggtg gcaatagtgt tgtcgcagaa aacagtacga
     2581 cccaaagaag tgtcgttcca cctaggataa gcaatgtctt acccattcct ggtttcttgc
     2641 ttcgatctgc tccgggatat tcatattgat agatgtattt gtacatcata taaaagaccc
     2701 agccaccgat tgcagaacct agaacgagtg tagaaaagcc ataagctggc gttgttccac
     2761 gtgaaaacaa tccggtaatc gcaatcatga tacttaatag accaagcaac agcaacgtat
     2821 tatctaacca catcaataaa ggggtggatg aattttttac ttctgctggc ttattgatga
     2881 ttgcttccgt tctttcagag actgtcccaa acaattgacg ggctgttctt ccgccttttt
     2941 gttctgcaac taaagtaggc agaatgtcat gaagggcttt tactttttct tcttcagaca
     3001 aattcgctgc ttctaaagat tttttcaaat caaaaatgta ctgttgattt cttttggtta
     3061 attttgtttc taaactattg ttttctttta cgattgctcg taattgttct ggttccatta
     3121 tctaatagtc ctccttagcc gttccttttc tcaacggtcg tcacaaaaga cattctacca
     3181 tattctacag ctagtttgat agccctttta cacattaaag cggaataaca ttacgtcgcc
     3241 gtcttgtacg acataatctt ttccttctaa tcgaacacgt ccagcttctt ttgctgcatg
     3301 catgtttccg taatgatcca agtcttcaaa agagacagtt tctgcacgga taaatccgcg
     3361 ttcgaaatct gaatggatga ttcctgctgc ttgtggtgct ttgattcctt ttttgaatgt
     3421 ccaagcacgt acttcttgtt ccccagcagt aaagtaagta gctaagccta acaaatcata
     3481 agcagcacgg atcaactgat ccaaacctga ttcttcgatt cctaaagctt ctaaaaattc
     3541 cgctttgtct tcttcatcta attctgcgat ttcttcttct gcacgagcac tgaccacgat
     3601 cacttctgca ttttcttctg cagcaaagtt gcgcacttct tgtacataat gattcgcgtc
     3661 tgcatctgct acgtcatctt ctgagacatt ggctacataa agaattggtt ttgtcgtcaa
     3721 taaaaatagt gatttaacga ttttttgttc ttcttcagta aattcgatcg ttcgggcaga
     3781 ttttccttct tccagtacag gtttgatttt atccagtaca gctagttctg ccactgcatc
     3841 tttatctttg gttttagcaa cttttgctac acgagtatag cgtttgttga tagattctaa
     3901 gtctgctaac actagttcca agttgatcgt atcaatatct tctagtggat caacacgtcc
     3961 ttctacgtga gtaatattat catcatcaaa acagcgaaca acgtgacaaa tagcgtctac
     4021 ttggcggata tggcttaaga attgatttcc caaaccttct cctttacttg cgccttttac
     4081 gattccagca atatctgtaa attcaaaagt cgtcggaaca gtttttttcg gatgtacaag
     4141 ttctgttaag cgttgcaaac gccagtcagg tacttctacc atccctacgt tggggtcgat
     4201 cgtcgcaaag ggatagtttg ctgcttctgc tcctgctttt gtgatcgcat taaacaaagt
     4261 tgattttcct acgttgggca acccaacaat tccagcagtc aatgccataa atttattcac
     4321 tctttctctg catttttttc aattactttt tttaatttct tttcaaaatc tcgacgggtc
     4381 atcatgacga tatgtccgca attcatacat ctgattttga tatctgcgcc cattcgaatg
     4441 acttcccagc gattcgtctg gcaagcgtga ggttttttca tttcaacgat atcgtgcaag
     4501 tcgtacataa aaatagcctc ctttttgttt attcttcatc aaattgaata ttcaagatat
     4561 caagaattcg agtcaaatct ttttgagaaa gatattcaat ctcgatctta cctttcccat
     4621 ttttttcttg gatagctaca cttgtaccaa atttatccat caaacgatct tcgctttctc
     4681 ggatataata tggtttttcc tttgctagcc ttgggatttt tttcttttct tttccatcat
     4741 tttgatttaa ctcagaaaca agctgttcca gttggcggac agttaaattt tcagttactg
     4801 cacgattggc taatttcaag atcaatgttt tattttttaa acctaacagc gtacgggctt
     4861 gtcccataga taaacgctga tcttgtacca tttccttgac aagatccggt aaagaaagca
     4921 agcgcaaata attggcgata taaggtctgc tctttcctaa acgactagct acttctgctt
     4981 gtgttagttt caggtttttc atcagcatct catatgcttc tgcttcttct aacggattca
     5041 gatcttctcg ctgcaagttt tccagcactg ctacttgcat catcgcttct tcgtcaaagt
     5101 ctctaacgat agcagggatc gtttcttttt cagctagttt ggacgctcga aaacgtcgct
     5161 cccctgcaat aatctcatat ccttttactg cggaacggcg tacgatgatg ggttgaaaga
     5221 cacctgattg ttcaatagaa ttagctagtt cctgcaaaga tacttcttca aatgttttac
     5281 ggggctgata cggatttggt cgcagctcat tcaatggtag ctggacaacg gtatcatctt
     5341 ttacatccac cgtctctaag ttttctaaat cttgaaacag tgcatcgatt cctcggccta
     5401 atcctttact tttcttcacg acttaccact tcctttgcca atgcttggta gacttcagct
     5461 cctcgagaac gtggatcgta gtcgatgatc ggttttccat gactaggtgc ttctgataaa
     5521 cgaatatttc ttggaatgat cgtttcgtaa actttttcgc ggaagtattt acgtacttct
     5581 tctacgactt catttcccag atttgttcgt gcatcataca ttgtcagtaa gactccctcg
     5641 atctctaatt ctggattaaa gtgtttttgg actaaacgaa ccgtattcag taattggctt
     5701 aatccttcca atgcgtaata ctcacactga acaggtatca aaatcgaatc acttgctgta
     5761 aatgaattga ttgtcagatg gccaagagaa ggcgggcaat cgatcaaaat ataatcatac
     5821 tgactgctta cttctgctaa agatcctttt aatcttgatt ctcttgccat cattgacgtc
     5881 aattcgatct ctgctcctgc taattggaga gtcgcaggca caatactcaa attttcgtgt
     5941 tcagtgatca aagttgcctc atcaattggc aattcattga ctagaacatc atagatgtct
     6001 cttgtcacat caggttttct gatcccgaca ccactggtag catttccttg tgcatccata
     6061 tccactagca atactttttt gccaaggcta gcaagacaag cacctagatt caccgtcgtc
     6121 gttgttttgc cgacgccacc tttttggttc gcaacagata ttatttgtgc catctcttct
     6181 cctcctattt gattggctgc ttattaggca gtccgggctt tcttgggtat ttctttgggg
     6241 ttggtttctt tttttgaata acgatgatat gccgttgatc agagacattc ggcaaggtaa
     6301 ccgcctcttc tttgaggaat ttaccaccta ataaagcaat cgcttgtttt gcttctgtca
     6361 attcctcttc actttttgct gctttcaatg cataaaaata gccttcttgt ttagccaagg
     6421 gcaaacacaa ttccgctaac acattcaaac gagcaaccgc acgagcagtg acaaggtcaa
     6481 atgatgcacg aaagtgagga ttttgcccaa atgtttctgc ccggtcatga tataaggaag
     6541 catcgattcc taataatcgg gttaactcag ataagaaggt tattcgttta ttcagagaat
     6601 caacgatcgt tatctgcagt tctggaaagg caatttttaa aggtatgcta ggaaaccctg
     6661 cccccgaacc aacatcgcat aaagataaag gttgcgtaaa gtctacttca aatgctaatg
     6721 taagtgaatc gtaaaaatgc ttcagatata cgtctttttc atccgtaata gctgtaaggt
     6781 tcatcttttc gttccattcg actaatgtcc gataataaat agcgaattga ttcatttgat
     6841 gatcggtaag ctctatccct ttttctgcca aggattggcg aaattcttct ggtatcataa
     6901 atattccttt cattgtgaaa cagcaattgt ttcacagatt ctctccctac tttaacgcaa
     6961 aatctctaga catgcaatcc ttccatatgg attcttaaaa atttgaaaag taagaaaagc
     7021 cgatagactt tttctttcta gtatgactca taaaatatac acagaagaga gaactagctt
     7081 tgagcaattt ctgcaaaaac tagttctctc ttctatttat tattacgtat tacgctggtt
     7141 cactgacttt cgcaatcttc ccttgttcga tatagaccat taggatactt aggtctgcag
     7201 ggtttacgcc actgattcgg ctagcttggg caattgtttc tggttggatt ttctgcaatt
     7261 tttgttttgc ttctgttgct aatccgttga ttgctgcgta gtcgatgttt tctggaatct
     7321 ttttcgcttc catgcgtttt aatttttcta ctttttccat ggcttttttg atgtagcctt
     7381 catatttgat ttggatttct acttgttcga tttcttttcg tgtcaattct tcattttctg
     7441 gaataaaccg tagaagttct gcataagtaa tctcaggacg tcttaaaaag tcgcttgcta
     7501 gtacaccatc ttttaatggt gccgcattaa tcgttgctaa aaagtcttgg acttcttctg
     7561 ttggtttgat tcgtacattc atcaaacgtt tgatttcttg ttcaactgct tcttttttct
     7621 tcaaataatt cttgtattga tcttctttta ctaagcctac acggtgaccc atttctgtta
     7681 atcgcaaatc tgcattatca tgacgtaaaa gcagacggta ttcagcacgt gaagtcagca
     7741 aacgataagg ttcgttcgtt ccttttgtta ccaaatcatc gatcatgaca ccgatgtaac
     7801 cgtcacttcg tttgatcact aaaggttctt ttccctgaac tttcaaagct gcattgatac
     7861 cggcaatcag cccttgtcca gccgcttctt cataaccgct tgtaccattt gtttgtccag
     7921 ctgtataaag attttcaacg actttagttt ctaatgtcgg acggagctgg tgtggctcta
     7981 ctacgtcata ttcgatagca tatcctgtac gcatcatttc tactttttct aatccagcaa
     8041 tagaatgaag catgttgact tgaacatcct ctggtaaaga agtagataaa ccttgaacat
     8101 agacttcttc cgtatttaag ccttctggtt ccaaaaataa ttgatgacgt ggtttgtctg
     8161 caaaacgcac gatcttatct tcgatggatg gacaatagcg agcgcctacc ccttcaacga
     8221 tccctgtaaa cattggggca cgatgtaagt tgtctcgaat aatttggtga gtcgtctcac
     8281 ctgtataggt cagccagcaa gattcctgat ctagtttata ggcactatct ggtgtgctga
     8341 agctaaaatg attcggctct ttgtcaccag gctgctcttc tgtaacagaa tagtcgatcg
     8401 tacttgattt tactcgaggc ggtgttcctg ttttgaaccg ttcgatagca aagccaagtt
     8461 cttttagatg attcgctagt ccaatagaag gttgagagtt attcggacct gatgaatatt
     8521 tcaattcacc gataatgatt tctccgcgaa gtgctgttcc ggctgtaata acaactgctt
     8581 tggcccggta gctcgctcct gtggacgtca ctactccatg acaaacacca tcttcgacga
     8641 tcagttcttc gacgattcct tgtcttagcg tcaaatttgg ttctttttcg atcgtatgtt
     8701 tcatttctgt tgaataagca tgtttgtctg cttgtgctcg caatgctcgg acagctgggc
     8761 cttttcctgt gttcaacata cgcatttgga tataggtttt atcgatattc ttacccattt
     8821 ctccgcctaa cgcatcgatt tctcgaacga caacgccttt agctggtccg cctacagaag
     8881 gattacatgg catgaatgct accatgtcca aatttattgt cagtaataat gtttttgatc
     8941 ccatacgtgc agcagcaagt gcagcttctg aaccggcgtg tcctgcgcca acaacgatca
     9001 cgtcatacgc atctgcttca taatgttcca atcgtttgtt cctcctttta ttttcctaaa
     9061 caaaattgac tgaataattg agtgattagc tcatcttgta cactgtcgcc gacaacttct
     9121 cctagatagt cccagcaacg cgtcatatct atttgaacga gatccacagg cattcctgaa
     9181 tcaatacctg caatgacttc ttcaagtgag agggaagctt tttctaacaa cgcgatatgg
     9241 cgggtattag aaacataagt agcgtctctt tctcctgttt ctccgctgaa gaataaatca
     9301 gaaatagctg attccaaacg atctagtccg tcgtttttag ctacggaaat tgaaaaaact
     9361 ggttcatttt cgatcaattt cttcaatttt tcttgttcca gctgtgctgg caaatctgtt
     9421 ttatttagaa gaatgatccg tttcaatcca cttgtcgctt ccaataattg ttcatcttct
     9481 gctgtcagtg gttcgctttg gtttagaaca agtaagatca agtctgcttc tgctaatgct
     9541 tttcggcttc tttcgacccc gatccgttcc accacatctt ctgtttcccg aattccagct
     9601 gtatcgatta gcttgagcgg taccccccga acattgacat actcttcgat tacatctcga
     9661 gtcgttcctg caatatccgt aacgattgct ttttcttctc ttaacaaatg gtttaacaaa
     9721 cttgatttgc cgacatttgg ccggccgatg attgccgtac ttaacccttc ccgcagaacc
     9781 ttcccttgct tggatgtagc aagtagcgct tggattcttt gttggatcat ctgcgctttt
     9841 tcaagcaata acttcgtagt caattcttcg acatcatcat attctggata atcaatattt
     9901 acttctactt gtgctaatgt ctctaaaatc tcttgtctca atgagcggat caaagcagac
     9961 agattgccgt ctaactgatt aagagctagt cccattgctt tgtctgtttt tgcccgaatc
    10021 aaatccatca cagcttctgc ctgtgaaagg tcaacacgac cattcaagaa tgctcgtttt
    10081 gtgaattcgc ctggctcagc taatcttgca ccttctctta acaatagctg caaaatttga
    10141 ttgactacaa cgatcccacc gtgacaattg atttctacta catcttcccg cgtaaatgtc
    10201 tttggtgctc tcatcacgga gaccatgact tcatcgacaa gctgattgct cttgggatca
    10261 acgatatgtc cataatggat cgtatgactt ggtacttctg ataatcgttt gtttccagat
    10321 tgataaactt tatctgctag ttgcacagct tgatcgccac ttaaacgaac gatactgatt
    10381 gcaccttctc caggtggggt agaaattgcc gcaatcgtat caaattctaa agtaatttca
    10441 gccatttttt cacttccttt ttcattttgc gcacaaaaaa agtgcccact ccacccttta
    10501 tgcacaataa acggtgaact tcgcactttg actgcttgtt cttgattaaa ttctggtcat
    10561 agattatcac acttgctata aggaatcaag tgtttaaaac aattagatga agttgagcct
    10621 gttctgcttc cattacctta tatttaattg tccttggatg tctctttgcg ttctttggtt
    10681 tattcgaaga gaagatactg tataaacaag gctttatctt tcttaaaaaa aacggtacac
    10741 taaatggtag cgctatctaa actatacact tttgaaagga gaaaatatgt gagcccgttc
    10801 agatctgctg cattggaaga agaaagtgac cctcggatca acgatgtcgt tttatctaca
    10861 gggaatatca accagaaata tatcgtccgc gatatcgttt ttgttgtgga atgcttagaa
    10921 gctgatttgt ttgacccatc ttttcccaaa gatgaccttt tcttaacaac gaagaggaaa
    10981 ttaaagacga aggtacttga atatggtgcg aatgcagtaa tcaactgtca ttttgagtat
    11041 cagcaaaaaa aacgcgacgg aaagtatttt ctggaacttt ttgcttatgg aacagtgatc
    11101 caatttatcc aaacaacgat tggttaaatg aaacagacaa aaaaagcgga tcaaaactga
    11161 agacagtttc gatccgcttt tttctatcaa agttagtgac tacttcatcg tatactctga
    11221 taagaatgat ttctagtaaa atatacttgg tttgaaaaac tttgtcaacg tgatttattc
    11281 aaaaagtaat ggaatcaata atatttttta gcaggttcta ctactaaata acgataaggt
    11341 tcctcacctt ccgaatgtgt ttttacatgt tcatctttgc ttaatgcagc atggatttgt
    11401 ttacgttcaa atgcaggcat tggttctaag aaaacaggct gccctgtccg ttttactttt
    11461 tcagccgtgc gatcagctaa acgctgaatg atctcttggc gcttttcacg gtaattgcct
    11521 acattgacta ctacagacag cttatttctt gctaaacgat ggatgaagac ttgtgccaga
    11581 tattgcaatg cattcagtgt ttttccatgt ttcccaatca gtatgccttg cttttgtgtt
    11641 tctaaatgga aaacaaccat attatttgat tctctttcca ttttgacaag agctggagca
    11701 tcaagctgtt ttgaaatttc agtaagataa actgccaatt ccgttaaagc tgcttcatca
    11761 cctagctcac ttacttcttc tgttttttct tcaagaatcg tttcgttgac tacctcgatc
    11821 tcagctgttg gttcttcttc caaaatttct tctacagtct gttctactgt ttcagaaact
    11881 ttttcgctga tacttggctg gatcgaaagc cgtgcattct ttcgacccat tcctaggaaa
    11941 cctttttttc cttcttccag tatttcgatt tcgacttggt ctttggccaa acccaaatca
    12001 gccagaccta gttggacagc ttcgtctatt gttgcagctt cataaatcgg cattagcatt
    12061 acctccccta ttatttccgt tttttcttag gattcattgc tttcttaaga gcacgttctc
    12121 tttcgcggat tctccgtgct tcttcttcac gctcttttcg aattttgaaa gggttgttga
    12181 tcagcagcgt ttgtacaact tggaaggcat tggaaactac ccagtataat gaaagaccgc
    12241 tggctaaatt gacacccatc actaaaatca ttacaggcat cacgaaattc atgatcttca
    12301 ttgatgcatt cgattcaatt tgactcatgc tagataaata cgtacttgca aaagtaaaga
    12361 ttgctgctaa aatcggcaaa ataaatgttg gatccgcgtt ccctaagttc aaccataaga
    12421 aatgcccttg tttcaactct ggtacacgtg aaatagattg ccataacgcc atcaagattg
    12481 gcatttgtac taataaagga agacaaccag catatggatt gacgccattt tctgcatata
    12541 aacgctgctg ctcttctctt aacttacttt gtgtttccgg atctcttgaa ctatattgtt
    12601 gctgcagtgc ttttaatttc ggttgtaatt cttgcgtttt acgcatgctc tttgtttgga
    12661 aatgcatcaa cggcaatagg atcacgcgga tgatcagtgt aaataaaatg atccctatcc
    12721 ctgcattacc aaatgacaac gccttgatcg cctcagcaaa gtaataaaca atataacgat
    12781 cccaaattcc tgtactctgt gcgctgactt ccccagttcc gcaaccggat aagacgatga
    12841 ccaaactaac gataccggcc atcaaaagta aacgtttgta tttcttcact tactcttttc
    12901 ctctctgtcg attatttttg caagttttaa gacgtggact aaattggagc gaacctcatc
    12961 ataagtcaac cccttcacac ttggtctagc aatcacaata aaatccaact ccggatcaat
    13021 ttcttctttt atagaataaa ctgctgctcg gatttttcgt ttgacttcat tgcggtttac
    13081 cgcgttgccg accttttttc ctacagataa accaactcga aaatgaggct gtcccggttt
    13141 ttccaaacgg taaacgacga atttgcggtt agcaaaagaa tctcccgtat ggaacacggc
    13201 ttgaaactct cgttcttttt ttactcgata cgcttttctc attagttttc tttccttttc
    13261 tatctctcac ttacgctcat aatcatacca taattacgat tgtcctttca tcatttccct
    13321 aaaaaagagt ataaaaaaaa ccactgaaac cccagtggcc taagctgaaa gaacttttct
    13381 tcctttgcga cgacggctag ctaacacacg acgtccattt ttagtactca tacgtttacg
    13441 gaatccgtga actttttgac gtttacgttt atttggttgg tatgttcttt tcatatattc
    13501 cacctccaaa atgttcttat ctattttata aacacagaca tactttgata gtataacgac
    13561 aaagtagtga ttttgtcaat ataaaataga agatcgattg ttaaaactta tccacaagat
    13621 tatttttttc tatgggaatc ctatgtggaa aaagttatgc acagggatta tccacagata
    13681 ataaaaggct ggttttacac agaaaacaca cgtgtaaaaa aagtaatcca caatcaggcg
    13741 atttcctgtg tattccgtct cctaactatt ggtatatctg tattttttat ccacagacgc
    13801 aagaggaaat ctattggttt ttacgagttt tccacctgtg cattagtggt gtggaaaact
    13861 ttttttgaca actgtgtttt tctctttttt ctttttgtcg attgtgtgga taattgtaat
    13921 taaaaaattt ttttcttcgg tttctgtgga aaactattga aaaaaatgct aaattagtac
    13981 tagttattca ctttttaatg aaggaggagg ccttatatgg tatccctcga tgctttatgg
    14041 aatgaattaa aagcaacata ccaaaaggat ttatcgcctg ctagctataa tacatggatc
    14101 gaaacagcga accctcgaac ccttgatcaa aaccagctcg ttgttgaggt tcctagcaag
    14161 attcataaag aatattggga aaaaaacctg gcgacaaaaa tcgttgaagt cggttatatg
    14221 ttatcaggaa atgagattat ccctcgcttt attactggcg aagaagcaga gcaagaagaa
    14281 gttatagaag aaaaaaatcc aaaagttgtg gcacctagtc cgctaaaaaa agccatgctg
    14341 aaccctaaat atacctttga tacgtttgtc attggtaaag gaaaccagat ggctcatgca
    14401 gcagcgctcg ttgtagcaga agatccaggt tctatttata acccgctctt cttttatgga
    14461 ggcgtgggac ttggtaagac tcacttgatg catgcaattg gtcatcaaat gctgcaaagt
    14521 cagcctaatg ccaaagtaaa atatgtaagc agtgaaactt ttgctaatga tttcatcaat
    14581 tcgatccaaa acaaaacagc agaagagttc cgtcaagaat atcgaaatgt tgatttactg
    14641 ctagtggatg acattcaatt ttttgcagaa aaagaagcta cgcaagaaga gtttttccat
    14701 actttcaatg ccttgtacaa tgaagggaaa caaatcgtac taaccagtga tcggttgcca
    14761 aacgagattc cgaaactgca agaacgcctt gtttctcgct ttgcatgggg gctgtctgtt
    14821 gatattacac cgccggatct tgaaacaaga acagcgattt tacgtaaaaa agcggcggct
    14881 gaacgtctag agatcccaga tgacacacta agctacatcg caggacaaat tgattctaac
    14941 attcgtgaat tagaaggtgc gcttgttcga gtgcaagctt ttgctgcgat gaatagtgaa
    15001 gatatttcga ctagtctagc tgcagacgca ctgaaaacgt taaaatcagg aaaaggacat
    15061 cctcaattgt caatcctgca gatccaagaa gaagtggcaa agtattatca catccaattg
    15121 aaagatctca aaggaaagaa acgcgtcaaa tctattgttg ttcctagaca aatcgctatg
    15181 tatttggcac gtgagctcac agataattct ttaccgaaaa tcggagctga attcggtggc
    15241 aaggatcata caacggttat ccatgctcat gaaaaaatcc agcagctgat ggattctagt
    15301 gtcagtatgc aaaatgaagt atcagaaatc aaaaactttt tactaaatta atttgtcgat
    15361 tctatcaact gaaaatagca tgtggataaa agaaaaaaac gttcaaaagt tatacacact
    15421 ttttccacag ctgttttttc gtttctctct tacgtttttt gagttttcca cagaatcaac
    15481 atgccctatt acttttatta ttctttttaa ctaataataa ataaacaaac attcaaagga
    15541 gatacatcat gaaagttact ttaaaccgag ctagctttat gcaggaattg caaactgttc
    15601 aacgagctat ttcaagcaaa accacgatcc ctattttgac aggtgtaaaa atcacactga
    15661 cacaagaagg tttgactttg acggggagca acgctgatat atcaattgaa acttttttgt
    15721 ctgttgaaaa cgaaaaagca aatatgcaaa tcgaatctac tggttccatt gttttacaag
    15781 cacgtttctt tagcgaaatc attcggagac ttcctgaaga aacatttact ttagaagttt
    15841 tagaaaataa acaagtagcg atcacttctg gaaaagcgaa ttttatcgta aatggattag
    15901 atgcagataa ctatcctcat cttcctgttg tcgaaagcca taaccagatg aaattacctg
    15961 tacacgtatt gactaaacta atcaacgaaa cagtttttgc tgtctcacaa catgagagtc
    16021 gtccaatctt gacaggtgtg catttcattt tatctgataa ttctttatta gctgtagcta
    16081 ctgattctca ccgtctaagt caacgcgtga ttccagtaga acaagcggct gatcattttg
    16141 atattgttat tcctggaaaa agtttgatcg aattatctcg ctcattaaca aatgaagaag
    16201 aaatcgtcga aatcagcatt atggaaaacc aagtgttgtt caaaacagaa acgatgtatt
    16261 tctattcccg tttgctagaa ggaaactatc cagataccaa tcgtttgatt ccttcaagtt
    16321 ttaatacaga ggttgaattt tctgttccta gctttttagc ggcgatcgaa cgggcttctt
    16381 tactttctca tgaaggccgt aacaacatcg ttcgtttatc tattcgtcca gatgcggttg
    16441 ttttatacgg aaattcgcca gaaatcggga aagtagaaga aagcttgagt tatacagcaa
    16501 gcagtggcga tccattagat atttctttca atccggatta tatgaaagca gcgttgcgtg
    16561 catttggaga tatgagcatc aaagtgaaat ttatctctgc tattcgtcca tttacattgg
    16621 aaccaacgga agatggcgtt caatttattc agctgattac tcctgtacgt acaaattaac
    16681 aatgattttc aatgttcttg aaaatttaat tgaaaagaaa tagaaaaaat aaaattttaa
    16741 acagaaggcc ctaaaatctc ttttaaggct ttctgtttat ttttgtttaa atcacctttt
    16801 ttagattaaa tcgtcttata tggaaaaata tatggataaa tttgttttat tgcgaaaaaa
    16861 aaggtataat agagtatact tttaataatg aaaatgaggg atgggcattg aaacaacaag
    16921 tcattctaaa tacggaattc atgacgttgg gccaaatgtt gaaagaagtc acagttatcg
    16981 gtagtggcgg acaagcaaaa tggtatttgg cagaaaacac tgtgttagtc gatggcgaac
    17041 cagaaaatcg tcgtggacgc aaactttatc ccggcatgat ggttgaagtt ccagaagtag
    17101 gaactttttt tatggtaaaa aaaggaaacg acgataatga ggctgaataa gctgtattta
    17161 aaaaattatc gaaattacga agagctgaac atcgaatttt ccaagaacct cgtcatcttt
    17221 ttaggagaaa atgcgcaagg aaaaacgaat cttttggaaa gtatctacgt attagctatg
    17281 acgcgaagtc atcggacaag tagtgagcag gaattgattg gctggactga cgatcaggcg
    17341 atgattcaag gagagatcac aaaaggttca tccactcttc cattagaaat cttattatct
    17401 aaaaaaggcc gaaaaaccaa gatcaaccat atcgaacaga aaaagttaag tacttacgtg
    17461 ggacaaatga atgtcatttt atttgcacca gaagatcttt ctttagtaaa aggcagcccg
    17521 cagatcaggc ggaagttttt agatatggaa ttaggccaga tcaatccaat ctatctttat
    17581 gatcttgtcc aataccaagc aattttgaaa caaagaaacc agtacttgaa gcaattgaat
    17641 gaaaaaaaac aaactgattt gctttatttg gatattttgt ccgaacaact agcagcgttt
    17701 ggcagcaaag tattgaaagc aagagcacag ttcgtcaagc gattggagtt ttgggctaat
    17761 tctttgcatc agcaaatcac ccatcaaaaa gagcagctgg aaatcgagta tctcaccgca
    17821 gtcgattctt tagaaacaca tacgcaagag caaatccaag aacaatttct cgcacttttg
    17881 aaccaaaata aaaagaaaga cctttttcgg ggaacaacga cagttggtcc tcatcgtgac
    17941 gatctttcct tttttattaa tcagaaaaat gtgcagactt atggatcgca aggacagcaa
    18001 agaacaactg cactaagtgt caaattagca gaaatcgatt tgattaaaga agagacaggg
    18061 gaatatccaa tccttttgtt agatgatgtg atgagcgagc tggatgataa tcgtcaactc
    18121 catctgctag aaacaatcga aggaaaagtt caaacgttct taacaacaac gacacttgac
    18181 catgtaaaaa ataaaatgac tgttgaacct gagatttttt atgttcaaca agggaaacta
    18241 aaaggagaag aaactgaatg acagaagaaa gaagtttagt agaacgcgct aaagagtatg
    18301 atgccagtca gattcaagta cttgaaggac ttgaagccgt tcgtaaacga cctggcatgt
    18361 atatcggttc gactagttct gagggtctgc atcaccttgt ttgggaaatt gtcgataatt
    18421 cgatcgatga agtattagca ggttttgcaa cgaaaatcca tgtgattatt gaaaaagaca
    18481 acagtatcac cgttattgac gacggtcggg ggatacctgt agatatccag gcaaaaactg
    18541 gacgtcctgc agttgaaacg gtctttactg ttttacatgc tggaggaaaa ttcggcggtg
    18601 gtgggtacaa agtttctggt ggacttcacg gagtaggatc ttctgttgta aacgcgcttt
    18661 ctacacagct tgatgtcaaa gtatacaaag aaggaaatgt gtactatcaa gaatatcgtc
    18721 gtggagctgt agtggacgac ttaaagatta tcgaacaaac ggatcgccat ggaacaacgg
    18781 ttcactttac gccagaccct gaaattttta cagagacaac tgagtttgat ttttcaaaat
    18841 tggctacccg gatccgtgaa cttgctttct tgaaccgcgg gatgcgtata tcgattgaag
    18901 acaaacgaga agaagaacca gtcatcaatg aataccatta tgacggcggg atcaaaagtt
    18961 atgtcgaata cttgaatgca aacaaaaccg tgatttttcc agaacctgta tatttggaag
    19021 gtgaacagca agatatcgca gttgaagtct ctatgcaata tacagacggc taccattcca
    19081 atatcatgag ttttgccaac aatatccata cgtatgaagg tggaacacat gaatcaggat
    19141 tcaagactgc tttaactcgt gtgataaatg attatgcccg aaaacaaaag ctgatgaaag
    19201 aaaatgacga caacttgacc ggtgaagacg tgcgagaagg attgacagct gttatttcta
    19261 tcaagcatcc tgaccctcaa ttcgaaggac aaacaaaaac aaaattgggc aattccgaag
    19321 tacggacagt cactgatcgg ttatttagcg aacatttcat gaaattcttg ctggaaaacc
    19381 caagtgtggg ccggcaaatt gttgaaaaag gattactagc ttccagagct cgtctagcag
    19441 cgaaacgtgc gcgtgaagta acgagacgta aaggtgcttt agagattagt aatctgccag
    19501 gaaaattagc ggactgctct agcaacgatc ctgaaaaatg cgaattgttc atcgtcgaag
    19561 gagattcggc cggcggatca gctaagcaag gtcgtaatcg agagtttcaa gccatcttgc
    19621 ctattcgtgg gaaaatcttg aatgttgaaa aagcaagcat ggacaagatc ttggcaaatg
    19681 aagagatccg gtctttattt actgctatgg gtacagggtt tggagaagat tttgatgtat
    19741 cgaaagctcg ctaccacaaa ttagttatca tgactgatgc cgatgtcgac ggcgcgcata
    19801 tccgtacatt gctgctgact cttttttatc gttacatgcg tcctattgtt gaagctggat
    19861 acgtctatat tgctcagcca cctttatacg gggtaaaaca agggaagaat attacgtatg
    19921 tacaaccagg aaaaaatgca gaagaagagt tgaaacaagt cgttgcttca cttcctgcct
    19981 caccaaaacc aagcgttcag cgttataaag gacttggaga aatggatgat catcaactat
    20041 gggaaacaac tatggaccca agcaatcgga tgatggcccg cgtcagtgtg gacgatgcta
    20101 ttgcagcaga tcagatattt gaaatgttga tgggggatcg agtagaaccc cgtcgtgcat
    20161 tcatcgagga aaatgcacac tatgtgaaga acttagatat ttaatcatga aggaggaaaa
    20221 agaatgagtg aagaaatcag agaaaacatc catgatgtca atctaaccag cgaaatgaaa
    20281 gactccttca tcgattatgc gatgagtgtt attgtcgcac gtgcacttcc tgatgtcaga
    20341 gatggtctga aacccgttca tcgccgtatt ttatacggca tgaacgaatt aggtgtaaca
    20401 ccggataaag cccataaaaa atctgccaga atcgtcggag atgtcatggg taaataccat
    20461 cctcatggag acattgcgat ctatgagtca atggtacgga tggcacagcc attcagctac
    20521 cgttatatgc tagtggatgg tcatggaaac tttggttctg ttgacggtga cggtgccgcc
    20581 gctatgcgtt ataccgaagc acggatgagc aaaatcgcta cagaaatgct gcgtgatatc
    20641 aataagaaca ctgttgattt ccaaagcaac tatgatgata cagaaaaaga accagttgta
    20701 ctacctgctc gcttcccgaa tcttttagtg aacggaacaa cggggatcgc cgtaggtatg
    20761 gccacgaata tcccacctca taacttgtct gaggtagtag cggctatcga tttgctgatg
    20821 gataatcctg atgtgacgac caatgaattg atggaagtat tacctggacc agattttcca
    20881 acaggtggtt tggttatggg gaaatcagga atccgaagag cgtatgaaac aggtaagggt
    20941 tctattaccg tacgtgcaaa agttgagatt actgagatgc caaatggtaa agaacggatc
    21001 atcgtgacag aattgcctta tatggtcaat aaagcaaaat tgatcgaacg gatttctgaa
    21061 ctgcacagag acaagcgtat cgaagggatc acagatttgc gggacgaatc ttcccgtgaa
    21121 ggtatgcgga tcgtcattga catccgtcgt gacgccagcg catccgttat cttgaataac
    21181 ttatataaat tgacatcctt gcaaaattca tttggattca atatgttggc gatcgaaaaa
    21241 ggtgtgccga aagttttgag cttgaaacaa atccttgaaa actatatcga acatcaacgc
    21301 gaagtcatca cacgacggac ggtctttgaa aaagaaaaag cagaagcaag agcacacatc
    21361 ttagaaggtt tgcgtattgc tttggatcat atcgatgaaa tcattgcgat tatccgcggg
    21421 tctaaatcag atgacgaggc aaaagcaaca atgatcgaac ggttcgattt atccgatcgc
    21481 cagtctcaag ctattttaga tatgcgttta cgccgattga ccggtttgga acgggaaaaa
    21541 atcgaaaatg aataccaaga actcttaaaa ctgattgaag atttaactga cattctggca
    21601 agaccagaac gtgttacaga aatcatcaag aatgaattag cagacttgaa tcaacgattt
    21661 ggtgataaac gacgcacaga attactagtc ggtgaggtct taagtctgga agatgaagat
    21721 ttaatcgaag aagaagaaat cgttattaca ttgacaaaca atggttacat caaacgtttg
    21781 gcaaataatg aattcagagc tcaacgaagg ggtggacgtg gtgtccaagg aatgggtatc
    21841 cacaatgacg acttcgtcaa aaatctggtt tcttgttcta cccatgatac attgttattc
    21901 tttacgaata acggaaaagt gtatcgggca aaaggatacg aaattccaga atacgggcgt
    21961 acagccaaag gaatcccaat catcaattta ctaggtatcg attcgagtga aagcatccag
    22021 gcaatcattg cggtgacggg agaagcagaa gaaggccact atctcttctt tacgacgcgt
    22081 caaggtacag taaaacgtac ttctgtcact gcttttgcta acatccgaag taatggtctg
    22141 atcgcaattg gtttgaaaga agacgatgag ctcgtcaatg tgctactgac agatggtcaa
    22201 tctaatatca ttattggaac acataacggc tactctgtca ctttcaaaga agaagcagtc
    22261 cgcgatatgg gacgtacagc ttccggggtc agagggatac gcctgcgtga ggaagattat
    22321 gtcgttgggg cagccttaat gaaggaaaat caagaagtat tagtgatcac agaaaaaggt
    22381 tacggaaaac ggacaaaagt atcggaatat ccagtgaaag gccgcggagg taaagggatc
    22441 aaaacggcga acatcactga aaaaaatggt ccactagctg gattgaccac tgtttctgga
    22501 gatgaagaca tcatgctgat tacagataaa ggtgtcatta tccgtttcaa cgtatcgact
    22561 gtttctcaaa ctggacgttc aacattaggc gtacgtttga tgaagatgga agctgatacg
    22621 aaagttgtca cgatggcagc agtcgaacca gaagctgcag aacaaacagt tgaagaacaa
    22681 ggatcagaag aataaccatc aagactaagt gtgtaagcta gcaagaataa ccgattaaaa
    22741 aatctggtta ttcctgctgg cttttttgat tagttgaatt tctcttaaaa tattcgaaac
    22801 tgttcattct gtattgcttt tgaccaaaat agagtgtata attagagctt gtgagtagtc
    22861 taattaatta gataactctc cttgctctta ggaacctaag agccgaaagt ccacagggag
    22921 gtgaatatca atggaaaaca cgaaatatga aatcatgtac attattcgtc caaacattga
    22981 tgaagaagca aaaactgctt taatcgaacg tttcgatgca atcttgaaag ataacggagc
    23041 agaagttatc gaatcaaaag attgggaaaa acgccgtttc gcttatgaaa tgaacggtta
    23101 ccgtgaaggt atctatcaca tcgttaaagt atcttctcca tcatcagcaa atgctgtcaa
    23161 tgaatttgac cgtcttgcta aaatcaacga cgatatcatt cgtcacatga ttgttaaaga
    23221 agaagcttaa ttgttgtttc atgtgaaaca atagaaaacg atgagaggag atgactattt
    23281 tgattaataa tgttgtatta gttggacgtc tgacgaaaga tccagatttg cgttacacag
    23341 caagtggtac ggcagtcgca acatttactt tggctgtgaa tcgtaatttt acaaaccaaa
    23401 gcggcaatcg cgaggctgat tttatcaact gtgtgatttg gcgcaaatca gcagaaactt
    23461 tagcaaacta tgctcgtaaa ggaacacttt taggtgttac tggacgtatc caaactcgtt
    23521 cttatgataa tcagcaagga caacgtgtat acgtgacaga agtagtagct gacaacttcc
    23581 aattgctgga aagtcgttct gcatctgaaa atcgccaaca aagcggcgga ttccaaagtt
    23641 ctggtcaatc agctggcgga tttggcggaa acaataactc taatcaaaca tctcaatcat
    23701 ccaacggtat gcctgatttc gaccgtgata cttctgaccc attcggctca agttcaacga
    23761 tcgacatttc agacgatgat ttaccattct aaacgaattt gataggaggg aaatagaatg
    23821 gcacaacaaa gaagaggcgg acgtaaacgt cgtaaagtag actacattgc tgctaaccat
    23881 attgaatata tcgattataa agatatcgaa ttactaaaaa gatttatttc agaacgcggt
    23941 aaaatcttgc cacgtcgtgt aactggtaca ggcgctaaaa accaacgtaa aattactgtt
    24001 gcaatcaaac gcgctcgtat catgggcttg ctacctttcg ttagcgaaga ctaataaatc
    24061 ctaggaattg aatgactgat ccttttgatc agctattcaa ttcctttttt tctgtaaaaa
    24121 taacctctat tcgtttagtc gctcgaatga cgaaagttat ggtaaaatca aattagtcat
    24181 atatgggagg aaaattatgg aaaataaaaa caatcattta tccccgaaac ctctcttcct
    24241 tatcctgttt atattaatcg aagtattggt agtcttaatt ttaccatttc gttttgttag
    24301 cgcttcggtc attctattga tgaatttatg gggtgttcgc tattggttga aaatgaaacg
    24361 ttggcttatt ttaaataaac gtgaaaaaat ccagcaggct tcgactgtag ctgaaaccaa
    24421 tctagcttat gtctcagaag tcatgcctgt aggagtgatt gcttatcagc catcaagcca
    24481 aaaaatcgaa tggctcaatc cattagcatt gaaaatcaaa gatcaaagtg gattaacaga
    24541 ggaagaattg ctagatactt tttttactgc aaaagaaaaa aagaaaaacc aagtctatct
    24601 agcagataaa acatttcagt ttgatatgga tacagtaaag ggtgttgttt attttaatga
    24661 tattacccaa aaccagttgt taaaactcca tcagacgaac agtcagccgg tgattggtat
    24721 tttatctatt gataactatg cagatgccat tgataaatta gatgacaaag agatttctta
    24781 tttgaactct ttcgtcacta cactagtttc tgactggatg aatgaatacc atgtcttttt
    24841 caaaagaatc aacgcagaac ggtttttctt cgtagcacag atgatcgatc tgaaaaagat
    24901 gatggataaa aaatttgatt tgctggatcg ctttcgagca gaagctgaaa aacagagctc
    24961 gcctatcacc cttagtatgg ggattgctta tggctcaaga accctcgatg aaatcggtgg
    25021 agaagctcag aataatttag atgttgcttt agtccgtgga ggcgaccaag ttgtcgtgaa
    25081 agaggtaaaa gaaggagcaa aacctgttta ttatgggggg aaatctgcta agaatgtcaa
    25141 acgcacgcgt gttcgttctc gagcgatgag taccgctttg cgtcggattt ttgatgaaac
    25201 aggcgatgtg tttatcatgg ggcacaagtt tccggatatg gatgctttag gctctgcttt
    25261 tggtgtcagt tgtctggcac gcttcaacca gaaaaaagct tacatcgtta tcaatgagaa
    25321 cgagatcatt ccagatgtag aacggtgtct tgaagaaatc cataagcatc ctgaattaga
    25381 aatgcaacta atctctccag aacgtgccat agagctgaaa aaagaagagg acctcctcgt
    25441 gatggtggat taccataaac cttctctatc gatctctcag ggcctatatg agcaatttga
    25501 taagattgtg atcattgatc accatcgtcg tggggaagag tttccaagta aacctctgct
    25561 gacttatatt gaatcttcag cttcttcagc ttcagagcta gtttctgagc tgatccaata
    25621 ccaaagcagc aaaacaaatc gcttagatcg ttttgaggcc acattattgc ttgcagggat
    25681 ggttgtagat acaaatagtt tcaatgtccg tacaactgca cgaacttttg acgttgccag
    25741 ctatttaagg tcttgtggag cagacgcatc cttagtacaa tatttattga gctcggattt
    25801 agcgacatac ttggagatca gcaatttgat tgctggaagc gactatgtga cttctgatat
    25861 tgttgtggcg gcagggacag acgaaaaaga gtataatagt gttatagcag caaaaacagc
    25921 agatacattg ctttcgatga tcaatatcaa tgcagctttc gtcattacaa agcggacaga
    25981 cggcttgatt gggatcagtg ccagaagtac cggcacgatc aacgtccaaa tcatcatgga
    26041 atccttaggt ggcggaggac acttcacaaa cgcagctact caactggaaa atacgacagt
    26101 tgaggaaaca aaacagaagc tatttgctgc aatcaaagaa aatatgaatc aaatatatga
    26161 taaggagtaa tccaaatgaa agtaattttt ttacaagatg taaaaggaaa aggtaaaaaa
    26221 ggggacatca aagaagttcc agcaggttat gcacaaaatt atctaatcaa aaataacttg
    26281 gcaaaagaag caacgaaaag cagtatcagt cagttagcag gacaaaagaa agcacaagaa
    26341 aaacacgaag ctgaaatctt ggaagaagct aaaaagttaa aagaattctt ggaaaaagaa
    26401 gatactgtag tcgaattgaa ggcgaaagca ggggaagaca gccgtttgtt tggttcgatc
    26461 ccttcaaaac aaattgctga agggttaaac aaacaataca aaatcaaatt agataaacga
    26521 aaaatcgaat tagaaaatcc gatccgttca ctaggctaca caaacgtacc tgttaagctt
    26581 catcatgagg tgacggctac gatcaaagtt catgtcgtac aagaataaca aatcttgata
    26641 gcgatgttca gctggtaagt ataaccagaa ggaaaaaaga caaaccgaaa tttatctgag
    26701 aaaacagctg cagcaatgaa agcaaaagct gcaaatcgat ttcagattga tttcggtttc
    26761 ttttttggga acagcaaaat agcataaaga aaacgagtat aataaaagag aactattttt
    26821 ttattacact ttcggtacga atgatgactt ttctctcatt ttcttgtaaa atgaagcaat
    26881 tgaagacaag gagcgatacg tatgaatgaa gtgtggcagg atcgaatccc accgcaaaat
    26941 attgaagctg aacaagcggt tttaggctct gtttttcttg acgcagaaac gatcattgat
    27001 gccatggagt acatagagcc aaaagacttt tatcgtcgtg gccatcaaat tatttttgag
    27061 acaatgatcg agttaaatga tcgtaatgaa gcgattgacg tggttactgt caaagaccgg
    27121 ctggaacaag ccaatttgtt agaagatgcc ggtggtttaa gttatctctc agacttggca
    27181 ttagcagttc cgacagcagc aaatatcgtc tactatgcga aaatcgttga agaaaaatca
    27241 ttgctgcgta cattgatcca gacagcaaca ggaatcgtta cgaaaggatt cgagcaagga
    27301 gaagaagttc aaacaatttt agatgatgcc gaacgcagta tcttggaggt ttcggaaaaa
    27361 agaaatcgaa gcggcttttt atctattgct gaagtactga actccacgat tgaaaatatc
    27421 gatcgactag ctcaaaacaa tgaagaaatc acaggtctac caactggcta tcaaggtctg
    27481 gataaaatga cagcaggttt ccagcccgaa gaactaatca ttcttgctgc acgtccagcg
    27541 gtaggtaaaa cagcgtttgc attgaatatt gctcaaaatg taggaacaaa aacagatcgt
    27601 tctgttgcga tatttagttt ggaaatgggg gcagaatctc ttgtaaatcg gatgctttgt
    27661 gcagaaggtt cgattgaggc cagccattta cgtacaggtc agctttcgga agaagaatgg
    27721 tcgaatctga tcgttgcgat ggggagtctt tctcgagcaa atatttttat cgatgataca
    27781 ccagggatca aaatctctga aatccgtgcg aaatgtcgga aactagcgca agaaaaaggc
    27841 aatctaggct tgatattgat cgattatttg cagttgattg aaggtaacgg taaagagaac
    27901 cgtcaacaag aagtatcaga tatctctcgc caactgaaaa aactggcgaa agagctgaaa
    27961 gtacctgtca ttgctctttc acagttatct cgtggagtag aacagcgaca agacaaacga
    28021 ccagtgctaa gtgatattcg tgaatccgga tcaattgaac aagatgcgga catcgtagcc
    28081 ttcttgtatc gagatgatta ttatgaaaga gaaggcggag atgacgatga tggaaacgag
    28141 ccaaaaaatg acaacgtcat cgaagtcatc atcgagaaga accgaagcgg tgcacgagga
    28201 acagttgaac tactgtttat caaagaatac aataagtttt cgtcgctttc gccaagagaa
    28261 gaattttaaa gaagaggttg tgagccttga gcaatcccga gagctctcgt aagaagatgc
    28321 aaggagtaac cgcaggaacg actagagtac ttaatttaaa cagatctcca aagggattgt
    28381 gacaaaagtc ttgtcacagt ccctttttcc gaataaacgg tgttcaatca gctgaccctt
    28441 ggtattagct cagcaaacgt caaaatatga ggaactattt tgacgtttgt ttctctaata
    28501 ctcaggtcaa aacgctgatc ttacgacctc ttttccttaa acggtgttct cctgccaact
    28561 cctcggtcac aagtcacaat atcatcaaac atgagaagca gttttttgaa attgctcctt
    28621 gtgactcaga gttaaacggc aggctcacaa cctctttctt aaacggtgtt caaaagcttg
    28681 catctgcggt aatacgccca atcaaacgaa aaatatgaga agctattttt gtttggctgt
    28741 tcttattact tgatgcagga cagctttttc acaacctctg attcacggtg ttccaaaagt
    28801 agcttcttcg aaaaataagt cgaaatttca caaaaatttg agaagcaatt ttcgtacatt
    28861 ccttcttatt tcttgaagct gaccacttct gttacaacct cttttttaat aaccattcta
    28921 aaaagataaa gttcgttttt taagcgtttt tataaaataa aaataaaaat cattcgattt
    28981 tatttgaaat atgtatctat tattgataca atggttcagg attaaataat atagaacgag
    29041 gtgttcacat gtcatctgta gtagtagtag gtacgcaatg gggcgatgaa gggaaaggaa
    29101 aaatcacaga ctttctcagt gaaaatgctg aagtgattgc acggtaccaa ggtggcgaca
    29161 atgcagggca cacaattaag tttaacggtg tgacttataa actgcatttg attccttctg
    29221 ggatttttta taaagacaag atcagtgtga tcggaaacgg cgttgtagtc aatcctaaat
    29281 cattggttaa agaattagct tatttacatg aaaaaggtgt agaaacaagt aatttgcgta
    29341 tttctgaccg cgcgcacgta atcttgcctt atcatatcaa attggaccaa ttacaagaag
    29401 atgctaaggg cgaaaataag attggtacaa cgattaaagg aatcggacct gcctatatgg
    29461 ataaagcagc tcgtgtaggt atccgtattg cagacttatt agataaagaa atttttgaag
    29521 aacgtcttcg tttgaatcta gatgacaaaa accgtcaatt tgtaaaaatg tttgattcag
    29581 aaccacttga ttttgacgat atttttgaag aatattatga atacggacaa caaatcaaac
    29641 aatacgtcac agatacttcc gttatcttga atgatgcttt ggatgcaggc aaacgcgtgt
    29701 tgtttgaagg cgcacaaggt gtaatgctgg atatcgacca gggaacttat ccttttgtta
    29761 cttcttctaa cccagtagcc ggtggggtaa caatcggaag cggtgtaggg ccttcaaaaa
    29821 tcaataaagt tgtcggcgtc tgcaaagcct atacgtcacg tgtcggtgat ggaccatttc
    29881 caactgaatt atttgatgaa acaggagaac aaatccgcga agtaggacga gaatacggta
    29941 cgacaactgg tcgtccgcgt cgtgtaggct ggtttgattc tgtagttatg cgccattcta
    30001 aacgagtttc aggtattacc aacctatctt taaactcgat cgatgtatta agtggattgg
    30061 aaacagtaaa aatttgtacg gcttatgaat tagatggcga actaatctac cactatccag
    30121 caagcttgaa agagttgaac cgctgcaaac caatctatga agaattgcca ggctggtcag
    30181 aagacattac aggatgcaaa acacttgcag aactgccaga aaacgcacgt aactatgtac
    30241 gtcgtatctc agagcttgtt ggtgtacgta tctcaacatt ttctgtcgga cctgatagaa
    30301 cacaaacaaa catcttagaa agcgtctggg cgcaaatcta aggataaaaa cgacgactga
    30361 atcaggacgt tcttaccaga gcgagtcgaa gtgtttatct atacgataat aataacttga
    30421 agaggtagtg aaagagacgt ttagccttat tgccgatagg ctgtctcttg aacactgttt
    30481 attcagaata agggactgtg acaaaaaccg tgtcacagtc cctttttccg aacaaacggt
    30541 gttcaaaagc ttgcatctac ggcactaagc ccaatcagat gaaaaatatg gagtgctatt
    30601 tttatctgat tgttcttact gcttgatgca gagcagcttt ttcacaacct ctccactaaa
    30661 c
//
LOCUS       DACDYI010000034        30431 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.034, whole genome shotgun sequence.
ACCESSION   DACDYI010000034 DACDYI010000000
VERSION     DACDYI010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.034"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..416)
                     /locus_tag="IWM52_001439"
     CDS             complement(<1..416)
                     /locus_tag="IWM52_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HAP9973696.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAAT"
     gene            557..3130
                     /gene="mprF"
                     /locus_tag="IWM52_001440"
     CDS             557..3130
                     /gene="mprF"
                     /locus_tag="IWM52_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP9973697.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            3284..4135
                     /locus_tag="IWM52_001441"
     CDS             3284..4135
                     /locus_tag="IWM52_001441"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAP9973698.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            4380..5318
                     /locus_tag="IWM52_001442"
     CDS             4380..5318
                     /locus_tag="IWM52_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAP9973699.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            5341..6021
                     /locus_tag="IWM52_001443"
     CDS             5341..6021
                     /locus_tag="IWM52_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP9973700.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            5994..6818
                     /locus_tag="IWM52_001444"
     CDS             5994..6818
                     /locus_tag="IWM52_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP9973701.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            6985..7803
                     /gene="purR"
                     /locus_tag="IWM52_001445"
     CDS             6985..7803
                     /gene="purR"
                     /locus_tag="IWM52_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAP9973702.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            8306..9679
                     /gene="glmU"
                     /locus_tag="IWM52_001446"
     CDS             8306..9679
                     /gene="glmU"
                     /locus_tag="IWM52_001446"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAP9973703.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            9773..10744
                     /locus_tag="IWM52_001447"
     CDS             9773..10744
                     /locus_tag="IWM52_001447"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP9973704.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      10750..10852
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            10918..11487
                     /gene="thiT"
                     /locus_tag="IWM52_001448"
     CDS             10918..11487
                     /gene="thiT"
                     /locus_tag="IWM52_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAP9973705.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(11568..12341)
                     /locus_tag="IWM52_001449"
     CDS             complement(11568..12341)
                     /locus_tag="IWM52_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973706.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            12608..14308
                     /locus_tag="IWM52_001450"
     CDS             12608..14308
                     /locus_tag="IWM52_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAP9973707.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            14278..14496
                     /locus_tag="IWM52_001451"
     CDS             14278..14496
                     /locus_tag="IWM52_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973708.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            14516..15406
                     /locus_tag="IWM52_001452"
     CDS             14516..15406
                     /locus_tag="IWM52_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAP9973709.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            15424..15708
                     /locus_tag="IWM52_001453"
     CDS             15424..15708
                     /locus_tag="IWM52_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973710.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            16195..16809
                     /gene="gmk"
                     /locus_tag="IWM52_001454"
     CDS             16195..16809
                     /gene="gmk"
                     /locus_tag="IWM52_001454"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAP9973711.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            16814..17119
                     /locus_tag="IWM52_001455"
     CDS             16814..17119
                     /locus_tag="IWM52_001455"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP9973712.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            17372..19780
                     /gene="priA"
                     /locus_tag="IWM52_001456"
     CDS             17372..19780
                     /gene="priA"
                     /locus_tag="IWM52_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAP9973713.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            19795..20286
                     /gene="def"
                     /locus_tag="IWM52_001457"
     CDS             19795..20286
                     /gene="def"
                     /locus_tag="IWM52_001457"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP9973714.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            20279..21217
                     /locus_tag="IWM52_001458"
     CDS             20279..21217
                     /locus_tag="IWM52_001458"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAP9973715.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            21217..22575
                     /gene="rsmB"
                     /locus_tag="IWM52_001459"
     CDS             21217..22575
                     /gene="rsmB"
                     /locus_tag="IWM52_001459"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAP9973716.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            22588..23328
                     /locus_tag="IWM52_001460"
     CDS             22588..23328
                     /locus_tag="IWM52_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAP9973717.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            23325..25394
                     /gene="pknB"
                     /locus_tag="IWM52_001461"
     CDS             23325..25394
                     /gene="pknB"
                     /locus_tag="IWM52_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAP9973718.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            25559..26458
                     /gene="rsgA"
                     /locus_tag="IWM52_001462"
     CDS             25559..26458
                     /gene="rsgA"
                     /locus_tag="IWM52_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAP9973719.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            26471..27121
                     /locus_tag="IWM52_001463"
     CDS             26471..27121
                     /locus_tag="IWM52_001463"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP9973720.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            27122..27760
                     /locus_tag="IWM52_001464"
     CDS             27122..27760
                     /locus_tag="IWM52_001464"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAP9973721.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            27915..28769
                     /gene="mreC"
                     /locus_tag="IWM52_001465"
     CDS             27915..28769
                     /gene="mreC"
                     /locus_tag="IWM52_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAP9973722.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            28773..29282
                     /gene="mreD"
                     /locus_tag="IWM52_001466"
     CDS             28773..29282
                     /gene="mreD"
                     /locus_tag="IWM52_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAP9973723.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            29537..>30431
                     /locus_tag="IWM52_001467"
     CDS             29537..>30431
                     /locus_tag="IWM52_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973724.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSA"
BASE COUNT        10013 a         5393 c         6574 g         8451 t
ORIGIN      
        1 tccgtggcag cttcattttt gcaaatcgca gaaacgcctg ctcctacgct gacattgaca
       61 ttcggcatca cacgtacgat ggaggcctga ggatcttcaa gcgctgtttc taaatctatt
      121 aatgattttc ctgaagcaat agaaatgaat aagactttct ggctatattc tcttagttct
      181 tccaatactt tttcaatgat ttttggtttc acggctaaaa gaacgatatc tgattgtgat
      241 gcaacttcct gattactttt gcatgcatgg acgccagttt cagcagcaaa tttttgtgca
      301 ttttcaaaat gtccactgga aacgaacacc tcttctttcg agactgttcc tttttttaat
      361 aaaccatcaa taatagcttg tgccatattc cctgtgccga taaatcctac tttcatgtta
      421 tcctctcctt tagcattagg atatctttct ttttctatca tcgcaagcaa agaacaggtc
      481 taaagtggaa aaattaaaat aaataagata acattggggt atgacacaaa agtatacaag
      541 ttaggaatgg gaaaatgtga aaaataaacg atacttgatg ctgcaatgga taaaggaaca
      601 tagtctgatt ttaaaactga tttttttcgg ctccgtgtta gtctttgtgg caaatcaagt
      661 cgcaaatatt gctaatggca tgagttggca agatatttgg caaaggatgg gacaacaaag
      721 ccgcatgacg atcctgctca tgattctggc tggactgctg ggtgtcactc cgatgttgtt
      781 gtatgattgg gtgacgatca gagtcttaga aaaacaagga aaaccaaaaa tggaaagaaa
      841 agagtttctt cttgctgcgt gggtaacaaa cacgatcaac aatctcgccg gttttggtgg
      901 tgtggtagga gcaagtctgc gggctagttt ttatggaaag cagacaaata gaaaaatggt
      961 tttagccact gtatcaaaag tcgcactatt tatgctgact ggtttgtctg tatggtcgtt
     1021 tcttacattt attgatgtct tttttattca gtcagaaagt atttttcgaa gctattggat
     1081 ctggcttttc ggcggtagtt ttttagcacc agtcattttc ttttttgctt atctaaagag
     1141 aaagacattg ttcttcgagt tttatccaaa aggcttgctt ggtctgctac tggcatcctt
     1201 tggacaatgg acgggagccc ttgcggtatt tttgatcatc ggcaagttga tgagaataga
     1261 tatttcgatg ctttcgatct atcctatgtt catgattgct acattgatag gaatgctgac
     1321 gatggtccca ggtggaatgg ggacttttga cgttttgatg atcttaggaa tgagtcaatt
     1381 gtctgttcgg caagatgtag cagtagtttg gctattatat tatcggttgt tttattatgt
     1441 tctacctttt atgacgggac ttctattgtt catcacacat acaggagtca aaatcaatcg
     1501 atttttagat aacattccgc gactgttttt ccaaaaggtt gcgcatatcg tgctcgtaag
     1561 tgctgtctat tttgcgggga tcatgatggt acttctatca acaatcacta atctgtctgc
     1621 aatcagtcgg ctatttcaag tattattgcc gttctcattc aattttttag atcagacagt
     1681 gaatctattg gttggctttt tattactcgg ccttgctaga gcactttcag taaaagtcaa
     1741 aaaggcattt ctgcctacga ttgctttact atcttttggt attttaaata caatcacacg
     1801 tacgttatct tggcagttga tattggtata tgtcttgatt ctgttagctg tgtggttatc
     1861 cagaaaagaa ttttatcgaa caaaattcgt ttattcttgg ggagctataa tctttgatgc
     1921 tagcttgttc ggctttttgt ttatcgttta cgcaattgca ggctatcaca gcggacgctt
     1981 tttagtcggt cgaataacag gaaatcattt tattttgttt ccttcagatg atgtgtggtt
     2041 ctcagggctt attggtttgg gcatctcatt gctagcttta gtggcgcttt atcaatattt
     2101 agctgaagat actacgaagc ttggcagtga gtacgaaaaa gaccgttttg accgtttatt
     2161 ggaaacatac ggaggcacag ctacaagcca tcagcttcgc ttgtcaggtt acagttattt
     2221 ttattaccaa gaagacgacc aagatcaagt cgtttttggt tatcagatca aaggaaacaa
     2281 atgctttgtt ttaggagatc cagttggcaa tcctaaaatg atgcgggcag caacgaatca
     2341 gtttatgaaa caagcagatt gtttaggcta tcagcttgcg ttttataaga tatcagaaag
     2401 ctacgtagtg atgcttcatg aattgggttt tcacttcacg aaagtaggtg aagcagggat
     2461 tgttgatcta gcaacagaag acttgtctcg gctgagtcag caacttgaat atcaacgttt
     2521 gatgaaagaa ggataccatt ttacttggta cgatcaactg ccggaagaat tacatcaagc
     2581 tgtaaatgaa gtttcacaaa aatggcttca aggtgcacga gaaaaatact tctctgtggg
     2641 acgatttgat aaatcatatg tattatctag cggtgtcggt attgtccgta aagatcaaaa
     2701 agtcgttggc ttcatcaccg ctcagccgat gacgaagaag caggcgggat atgatttgtt
     2761 gcgggtctta cctaaagaat ctgaggaatt agctgactat ctgttagcca atctgtttat
     2821 tgtatacgag aaaatgggct actcggaagc caatctaggc ttagctccat tagctaatgt
     2881 tggagaaaca gatttttctt tctttcaaga aaaagtcatg catatcgtat ataattatgg
     2941 taattttttc tattccttcc agtctgctta tgaaaagaaa ttaaaatatg tcgatcattg
     3001 ggaaggacgt tattttgctt ataggaaagg aagtaatttc ggatttgcta ccatgcagct
     3061 actcagtctg ataggaaaag aaaaaggaaa tgttccgagt ttggcagaag aagtgctcac
     3121 agaattataa gaatcactag aacttgtttg gattatgaag agaaaattct gtgaaagctg
     3181 ttgaaacgat gacaaaatca accactaggc agacataagc tatactttta tttttcccta
     3241 tgatagaata cttgcgactt agacttttcg aatgggtgag aggatggaat tgattgaaaa
     3301 agcgccagcc aagataaatt taggactgga tgtattatat agacggcaag atgggtatca
     3361 tgagttggaa atggtgatgt cgagcgtaga cttggcagat catctatttt ttgaagaaat
     3421 aacagaagac aaaatcattg tagaaacaaa taaagcattt ttaccggtag atgaacggaa
     3481 caatgtttat caagctgctt tattgctaaa aaataaatac aatatcaaaa ccggtgtcaa
     3541 gatctttgtt aagaagaata ttccagtagc agctggatta ggcggaggaa gtacagactg
     3601 tgcagctgct ctaagaggga tcaaccaatt atggaatctg ggctgttcgt tagaagaact
     3661 ggcagaaatc ggtttggaag tcggtacaga tgttccttac tgtgtctacg gaagtacggc
     3721 atttgtcggt ggcaaagggg aagaaatcca agtattacct tctatgccac agtgctgggt
     3781 tgtgttggtc aaaccaagaa tgagtgtctc tacacgtaca gtgtttagag aagtagatac
     3841 gcaagccatc caacatcaag acatccacgg acttgctgaa gccatccgag agcaagatta
     3901 tcagcagatg atcaaaaaaa tgggaaacag tttagaagat ataacgggaa agcggcatcc
     3961 agtcatccat caaatcaaag agcgtatgat caagtacgga gcagatgctg cattgatgag
     4021 tggaagcgga ccaaccgttt ttgccttgtg tcaacattat tcaagagcgc agagagtata
     4081 taatgggtta aaaggttttt gtgaggaagt ttatttagta cgaaccctgc aataaaatat
     4141 aagaagtaaa ataggtgatg aaaaactgag gaatcctaaa atggtattct tcagtttttt
     4201 attgtagtga aatggctaga tatgagcagc aactcatcca ttttacgatt taatcaagaa
     4261 tatacatgct taaatcagaa attgatggat ttctattgac tgtgtttttt tattttgata
     4321 cattagttca cgttaataaa tcgtaaaatt ccgatttgtt cattgaaagg agcatataaa
     4381 tgaaaaaata tcttttgaca gctacggtaa tgataggtgc tttactgttt gcagcatgtg
     4441 gaaatacgaa caaggaagca gacaaaaaag aggatttgac gatcgttacg actttttatc
     4501 cgatgtatga ttttacaaaa gagatcgtag gggatgaagg aaacgtgaaa cttttgatcc
     4561 ctgctggaac ggagccgcat gatttcgaac caagtgcaaa agaaagagca gaaatttccg
     4621 atgcagatgt tttcgtgtat aatagttcag acatggaatt ttttgtggat tcgttaaagg
     4681 attcagtgga tagcaaacag accttgatga tcgaagcagc taaaggaatc gaccgtttag
     4741 aatcgcaaga agcagacgaa catgaagaat cagaagaagg gcatggtcat tcacatgaat
     4801 acgatccaca tgtttggctc gatccggtat tagctataaa agaagtcaga acgattgctg
     4861 aagaattagg cgaaaaatat ccagataaaa aggaaacttt tacgaagaat gccgatgctt
     4921 atatcaaaaa gcttgaagca ctggatcaaa aatactccga tgagctaaaa gatgcaacaa
     4981 accgcacatt tgtcactcag catgctgctt ttgcttattt agcgaatcaa tacgacttaa
     5041 aacaagtagc gatttcgggc gtttcaccag atcaggaacc cacaccaagt cgcttagctg
     5101 agctaaaaga atttgtgaag aaaaacaaca tcaaagtgat ttattttgaa gaaaatgcct
     5161 cttcgaaggt agctgagaca ttgtcgaatg aaacaggagt gaagctagaa gtgttgaatc
     5221 ctttagaaag cttgactaat gaacaaatca aagcaggcga aaattatatt tctgtcatgg
     5281 aaaaaaatct gaaagcttta aaagaaagta tcaattaagt tttagaatgg aggttgtaag
     5341 gtgcgttata tcgaaatatc tgatttgacc ttttactatg atgatgaacc tgtactagaa
     5401 gatgtctctt atcatgtaga tgcgggtgaa tttgtcattt taactggaga aaatggagca
     5461 gccaagtcaa ccttggtgaa agcatcgtta ggattactga aaccttcaaa aggaatgata
     5521 aagattgctg agaaaaatca agaaggaaaa aaattaagta tcggttatat tcctcaacaa
     5581 gttgcatctt tcaatgcagg ttttcctagt acggtgatcg aattagtccg ttcaggaaga
     5641 tatccaagag gccgctggtt caagcgtctc tctgaaaaag atcatcaaca tgtacagcgt
     5701 gctttagaag ctgtagggat gtgggatatg cgaaatcggc gaataggtga attgtctggc
     5761 ggacaaaagc agcgaatcag tttagcgcgt atttttgcta ctgaccctga cttatttgtt
     5821 cttgatgaac caacaacagg tatggatgaa gaatccagaa atgaatttta tcgtcttctc
     5881 cgacatagtg cgcatgtcca tgggaaatca gttctaatga tcacccatga tcatgaagat
     5941 atcaaacagt atgcggacag acagatccga ctggtgagaa aggaggattc tcaatggcgt
     6001 tgctttcata tgaattcatg agaagagcgt ttcttgccgc tatcttcatt gccggaatcg
     6061 ccccgatgct aggtgtgttt ttagtgatca gaagacaatc gctgatggct gatacgcttt
     6121 ctcatgtctc tttagcaggg gtagcgttag gattcttttt gaaccttaat cctacactta
     6181 cgaccatgct agtcgttgta ttagcagctg tgatattgga gtatctgcga acgatctacc
     6241 gaacctattc agaaatctcc attgccattt taatggctgg tggtttggcg ctagcgttag
     6301 tattgatgaa tttgagcgga gggaattcag caacaagtat ccaatcctat ctgtttggtt
     6361 cgatcgtgac cattacatgg gagcaagtca tctttttagg aatcttattc gttgccatcg
     6421 gtatattgtt ctttttattc aaacggccga tgtatgttct gacttttgat gaagataccg
     6481 cccacgtgga tgggctaccc gttcatttga tgtcgatgat gttcaatgtg attactggtg
     6541 tggctattgc tgtgatgatc ccgattgccg gtgcactttt gatatccgca atcatggttc
     6601 ttcccgcagc aatcggtatg cgattaggca aaagcttcaa tgtcgtgatt gctatcagtg
     6661 tttgtgtagg atttctaggg atgctgtcag gactaactag ctcgttttat ctagatacac
     6721 ctccaggagc aacaatcaca ttagtcttta ttgctttgtt cctgattgta aatatcatca
     6781 aacgaatatt ggttgccttc agaagaaaaa ataactaaat tataaagaaa agaagcctta
     6841 taaaaacgaa aaatttttta taggcttctt tttattttaa taaataccga actattttct
     6901 tattttttca taaaatgctc gaatttttga agaaaaactt atataatgta aaacgataaa
     6961 atttcttaaa aggagttgga ataggtgaaa gtacgtcgca gtgaacgtct agttgacatg
     7021 acacagtatt tattgaatca cccgcatgaa ttgatttcct taacaagctt tgccgaacgt
     7081 tatgaatctg ctaaatcttc tatcagtgaa gatttgggaa tcatcaagaa aacatttaaa
     7141 gaacgagggt atggaacctt ggaaacaatc ccgggtgctg caggtggtgt attgttcatc
     7201 cctgaaattt catatgaaga ggcgaaaaaa tatattgaat ctctagcaga acgtctatca
     7261 gaacaagacc gtctgcttcc aggaggatat gtttatctct ctgatttgtt aggagaacct
     7321 gaactgctgc gtcaagttgg aagaatcatt gcttctaaat accttggtga aaaaatcgat
     7381 gctgtaatga ctgtagccac aaaaggcgta cctattgccc aagctgtgtc ctattatctg
     7441 aatgcgccat tcgttattgt acgccgggac tcaaaaataa cagaaggatc aacagtaagt
     7501 gtcaactatg tgtctggatc ttctgaaaga attgaaaaga tggaactgtc taagagaagt
     7561 ttgaaacgtg gttcacgtgt ccttgtagtg gatgatttca tgaaaggcgg cggaacagtc
     7621 aacggaatga agagcttgat tgaagaattc gaatcagaac ttgttggcat cactgtattt
     7681 gccgaatcaa aattcaacgg tcatcgcgcc atcgatgatt atacgtcact gctttatgtc
     7741 aaagatgtag atactcaaac gaaaaatatt acggttgtgc caggaaatta tttttcagaa
     7801 taagaggttg tgaaaaagcc gcttagtttc gagtagtaag aaaaattttg tgaaaatggc
     7861 tttccacatt ttttcataaa tttggcttaa taccgagaaa ctggcttttg aacaccgttt
     7921 atctagaggt tgtgaaatca gcgtcctgtc ctgagtatta gctcaataaa cggaaaaatt
     7981 gctcctcata ttttgccgtt tattgagcta ataccgaagg tcagctgatt gaaaaacgtt
     8041 taatgaagag gttgtaagcc taccgtttaa ctctaagtca caagaaacga tttcaaaaaa
     8101 cagcttttca tgttttatga gtagtgtgac ttgtgaccga ggagttggca ggagagcacc
     8161 ggttaccaaa tagataggcc cgttatggtc tatctatttt tttatccaga aaacgctggg
     8221 ataaaagaaa atcacttgtt ttgttgaaaa aatgggtata ctgggataaa ctgaaatcag
     8281 caaactatat ttaggagtga cgcagttgga cgcacgttat gcaattattt tggcggcagg
     8341 gaaaggtacc cgcatgaagt caaaattata taaagtttta catcccgttt ctggtaaacc
     8401 tatggtagaa catatcatta atcgggtcag tgaaacgaaa ccagatgaag tgattacgat
     8461 cgttggacac ggagcagaac aagttaaagc acaactagga gaacgcagta aatatgcgtt
     8521 acaagcagaa caattgggaa caggacatgc tgttttgcaa gctgcttctt tcttggaagg
     8581 aaaaaaagga acaacgttag ttatcagtgg cgatacacca ttattgacta ctgaaacgct
     8641 gaacaatctt tttgaatatc accaaggaaa aaatgcaagt gcaacgatct tgacggcaca
     8701 agcagaaaac ccaacaggat atggacgaat catccgtgac catattggga tcgtagaaaa
     8761 aatcgtggaa caaaaagatg caacaccaga agaagctctt gtacaagaaa tcaatacagg
     8821 aacttactgt tttgacaacg aagctttgtt cgatgcgtta agcaaagttg gaacaaacaa
     8881 tgctcaaggt gaatactatc taacagatat catcgaaatc ttaaaagaag aaggccatac
     8941 agttgctgct tatcaaacgg atgattttga agaatcaatg ggagtcaatg atcggatcgc
     9001 gttagctaaa gcaaatgaga tcatgagaaa gcgaattaac cagatgcata tggtcaatgg
     9061 cgtaagcttt gtcgattcag caacgacata catcgatgca ggcgtagaaa tcggtccaga
     9121 cacattgatc gaagcaggtg tacaaatcca aggaaataca gtcattggtt ctgattgtgt
     9181 catcgggtca cattcaaaaa tcgtagacag taggattgag gatcacgtcg tcattgaaaa
     9241 ctcagtcatt gaaagcagcc atgtcaagaa acatgcagat gtgggacctt atgcacattt
     9301 acgaccaaaa gctgaaattg gcgaaaacgt ccatatcgga aactttgtgg aagtgaaaaa
     9361 tgctcaaatc ggcaaaggaa ctaaagtcgg tcacttgact tatgttggtg atgctacgct
     9421 gggagaagaa atcaatgtcg gatgtggtgt tgtcttcgtc aactatgatg gaaagaacaa
     9481 acatcatacg actgtcggcg accacagctt tattggttct agcacgaata tcatcggacc
     9541 tgtcgaggta gctaaaaaca gttcgattgc tgccgggtca acaattacag acaatattcc
     9601 agaatatgcg ctggcaattg cgagagcaag acaagtcaat aaagaaggat atgcaaaaaa
     9661 actcccttat ctaaactaag acttttgcaa aatcacttga tttatttaaa aaatatgact
     9721 actatttatt aggtagaatt ctagagttgt gaaaaagaaa gtggaggtcc ccatgtcaaa
     9781 acattacttt gacccaagat taaaaatttt tgcgttaaat tccaatcgcc cattagcaga
     9841 gaaaattgca gcagcagtcg gcgtcgagtt aggaaaatca tctgttactc aatttagtga
     9901 tggagaaatt caagtaaaca ttgaagaaag tattcgtgga tcgcatgtct atgttattca
     9961 atcaacgagc agcccagtca atgataactt gatggagttg ctgatcatga ttgacgcact
    10021 gaaacgagca agtgcaaaaa caatcaacgt agtcatgcct tattatggct atgcgcgtca
    10081 agaccgtaaa gcacgtgcac gggaaccaat cacagcgaaa ttagtagcaa acatgattca
    10141 aaaagctggt gcaacaagaa tgctgacttt ggaccttcat gcagtccaaa tccaaggttt
    10201 ctttgatatt ccagtcgatc atttgatggg tgcgccactt attgccaatt atttcttaga
    10261 aaaaggaatc aaaggcgatg atgttgtcgt tgtttcacca gaccatggtg gtgtcacacg
    10321 ggcacgtaaa ttagcggaat ttttgaaatc tccgattgcg atcatagaca aacgccgtcc
    10381 aaaagcaaat gttgcagaag taatgaatat cattggacaa gtcaaaggca aaacttgtgt
    10441 actgatcgat gatatgatcg atacagcagg tacgatcaca ttggctgcca atgctttgaa
    10501 agaagctggt gcaactagtg tgtatgcttc atgtacacat ccagtattat ccggccctgc
    10561 tttacaaaga atagaagatt cagctatcga gcgtctagtt gttacagact cgatctatct
    10621 tccagaagat cgtaaaatcg acaaaatcga tgaagtaagt gttggcggac taatgggaga
    10681 tgcaatcaaa cgtatccatg aaaacaaacc agttagtcca ttgtttgaaa caaaaaataa
    10741 ataataaata aactcacaaa ggggagtcca attgggctga gattgaatct atttctaaac
    10801 ccttcgtacc tgtatcggtt atgcgagcgt aggaattgtg aataaacagc tgtttgacta
    10861 ttttcgaaag ctgcttcctc ctttgtgact tttttattca aaggaggaag tttttttatg
    10921 ggaaaaaata gagtttgggt agaaggaacg attgttgcag cgttagcgat ggtgttgtcg
    10981 ttgattccga tccagatcgg cagcagtttt tctatttcat tagggcagat tccattgact
    11041 ctttttgcgt taagaagagg gacaaaggca gggatgctgg caggtttcat ttggggcatc
    11101 ctacattttc cgttgggaca agtttattat ttaagtgtcg ttcaagtgtt gattgagtac
    11161 ccgattgcct atacttttgc aggcgcagca gggctcatgg cttcaaatgt ccaaaaaggc
    11221 tatgtagacg ataatacgaa acaaattaga aaaagcatcg tgtttggtgc attgattggc
    11281 gcactttcca gatatttttg gcattttatt gcaggagtgg ttttctgggg agcgtatgca
    11341 ctatggggga tgaatccttg gctcttttct ttcgtaatga atggtgccag cgggttgagt
    11401 acagctattg taacgatcat cgttttatta gggatccaac aaacgactcc ttcgattttt
    11461 gtgccagata aacgacagga gctgtaaata aaaaagaagc aagaaacttg agacagaatc
    11521 atgtcctagt ttcttgtttc ctttttattt tttgataaga aatagatcta cttcaatggc
    11581 agataagaaa gatcggcatc ttctaccaat tgccagtgtc gatcggtgac agagtagatt
    11641 aaagtagcga tgatttcctc ttctcgatac cgggtgcctt ttaatttcca aatataatct
    11701 tcaatcacat agattgggat acgatattta ccatcgatca aaccaacttg atcaaaagaa
    11761 aaaatgatcc cttcctgcat caaattacgc atttctttca ataattccac actgattttt
    11821 tcaacaggtg tttctttttt gaaaaaagat ttttttacat tgatcttatc tgacaagatc
    11881 gaacttaatt tactagcaaa aatcctataa aattcatcta aatagcgata ataaatccga
    11941 ttcagctgat cattaagatc gaagtataca aaattattct gtagtttata aaaaaacggc
    12001 gaatgaagat gtgttttcat atgaccaaaa tataataatt ctgaaatttc taaaggtgtc
    12061 aattctttca gcatcgttaa atcagtaaag tcgatccact tcaattcatc gaatccacgc
    12121 ctgttacgag aagaggaaaa gaaacgctga atattttctg taccacggat tactttcagc
    12181 cctgtatggg attcatattc tccagcagaa gcagatggat caagcaagag caagttttga
    12241 ggaaaatgca cgatactgtt tgcaaagtca gtagcagtca gcccttttgt taaaacagca
    12301 ttacttgtgg tatcaatatg tacataaact gactcagtca tcatgttcct cctcttattt
    12361 cctcttaatc tcttttattt tacaacattt gcattaaaaa agcttgtttc ttttatttta
    12421 aaattccatt acaaaatcac gttttcattg aaaacactct cattttcaat taatttttgt
    12481 gatttttgta tttttttatt taaaggaggt tttatgaatt gttttcttat ggaatctaca
    12541 aagtcgaata cgagactcat gaccttttta tgacgtactg gtatacttga agaaaaagga
    12601 gagcgcgatg gagataaaaa aagtttactt ttcacagaaa atgtcgctgt attcgcctat
    12661 ccagcaagca aaacaacccc ttccattttt tcaaagtcat caagaaagta gagacgcttt
    12721 ttggcgttct gcaaaaaagc aatgtgcttt taaagcagta tcgaaaaaat ataccgtcta
    12781 ttttactatt gctaaaagta gaggagatgt tatttttaat aatctgttga ttgaaggtga
    12841 aacttttgaa tggaaaaaat tttttcggta tatggaagct tgcgctcgtt tttttattaa
    12901 agaaaactgt tgttttttat ttcaatctcc tctatcagaa gaatggttgc gtatttttca
    12961 taaaaacggc tatcaaggaa ccacacaatt aaataaaaag ctggtctatc atacagcact
    13021 tgttttaggt ggaggaggag cgcacggtgc ttatcagatt ggtgtgtggc aggcgctgaa
    13081 ggaacatgat atcaactttg agattatcac aggtacctcg gtaggtgcgt tgaacggcgc
    13141 attgatttta caaggagata tgaaaaaagc tgtgaattta tggaaaaagc tgtcgacaag
    13201 acaagtgctt gcgttaccag aaatggctgc agttgaagac cttcgtgaac gcttctatcg
    13261 agaaagacgc caaatgacga aaacagcact aattgaaggc ggggtttctt ctgctccatt
    13321 ggaaaagatg gtcaaagaca atttggattt gtccttactc aaacataatc ccaaaatacg
    13381 tttatatacg gtgtctacta aactgccaga actagcggaa gtagtgactg atatccaaca
    13441 gacaaaaaca gaagaaatac cagattggat actcgcctct gcttcatttt atcccgctat
    13501 ggcttatcga aagatcggaa aaaataaata tgcagacggt gggtatcgca acaaaatacc
    13561 tattgatatt gcgattaaca aaggagcaac agaggcattt gtcgtagatg tgcaagggcc
    13621 aggcccagcg aaaaaaattc gtatgccaga catttttatc cattggaaat gtcaaacatt
    13681 gtggacacta ggcagctttc tgctttttga tagccaaagg aaccaattaa atttacaatt
    13741 aggctatctg gaaatgaaaa aacgattagg ctattactat gggaactggt atacatttca
    13801 ttctgttaaa cctgcaagaa catactggcg aggatttttg tcttacttaa caaaagaaat
    13861 tcagttagaa ttatcttttt tcaaaagcat aaaattttgg caaaaactgc gtaatttata
    13921 taagaacagg gtcgtaccag agacatgcgg attggcgatg ctagaacttt tagcaaaaaa
    13981 acatttttta ctgcccaatg aaatctatca agtagacatg atgattcaaa tgatctgcca
    14041 acaagatata aaaagtatgc cggatgattt attggcacaa attggtcaat taagtactga
    14101 agaatggcga aggtatcgaa aataccagca aaaaatacaa aatgagcgca cgaaaactgt
    14161 ttattttgat tatctgctac aaggaaaacg caaggatcgt ttgcagcatg aattgcttaa
    14221 gcaaccagtt gacacattac tgatcctata tttatattat ctcaaggagg aacaaccatg
    14281 gcacaagaat tttcatatga aatcgtagaa gaaatcgcta tattgtcaga aaacaacaag
    14341 gggtggcgta aagaactgaa cctagtcagc tggaatggtc gcccgccaaa attcgatctt
    14401 cgagattggg cgccagacca tgaaaaaatg gggaaaggcc tgacattgac gaacgaagag
    14461 tttgaacaac tgcaaaaagc aatagaaaat atgtaatgag gaaggaccct gtcgaatgaa
    14521 aagtatgact ggttttggca aagccatccg cgaaacaaaa gcgtatcagt tagatattga
    14581 aataaaaagt gtcaaccagc gttttctaga tgttcaaata cgaagtccta aaatcctgaa
    14641 ctttgttgaa aacgattttc gtcagcttat taaacagcag ttaagccgcg gtcgaatcga
    14701 agtatttgtg aatctagctt atattggcaa aaacgaaaaa cagatcgtca tcaactggga
    14761 attgattgat tcacttatca ctcaattgac agacggaatt caacaacgat acggtagtca
    14821 aaatcaagtg tcaattagtc ggatggcgga acgcttcgtg ctggacgaaa acttcgtaac
    14881 ggtagaagaa aaaccggaag agagcatgga agaattggca cagttagctc tagatacatt
    14941 gaaaagcgca cttgattcta ttgaggcgag tcgagaaaaa gaaggtcaag ctttagcttt
    15001 agtgttgaaa aagaatactg aagaattcaa agaagtacta aaaaaactcg atagttttgt
    15061 agaaatctat gaaaaggaat ttcaaataag gtatcaaaaa aagctggagg aatttcttgg
    15121 agcgaaagtc gatgagcaac ggttgttgac ggagctagcc atcttgttgg aacgcggaga
    15181 tattcacgag gaactggatc gtctggcgat ccatgtacaa aaactagatg aacttatcga
    15241 agcacaatgt cctgtaggtc gggaattaga tttcctgatc caagaaatga atcgggaaat
    15301 caacacgatc ggttccaaat caaatgcaat cgaaattaaa aatcaagtcg tccagtctaa
    15361 gacgatctta gaaaaaatca gagaacaagt acaaaatatt gaatgacgaa agaggtagag
    15421 ataatgaaat attatgacgt aacgttccac gaattaagtg gaaaaactgt ggttaaacgg
    15481 gatatccctt ctgaaaaaaa cggctttgac gtatggaaag atgcctgtgc agactataat
    15541 gagaacgaac tgtttatttt gatcaacgac ggtgcttacg tgacgatgaa tcggaaattt
    15601 attgttcgaa ttgacacaga agaggtagaa gatccgacag aaaaagcacg ttctcgaaaa
    15661 gatgagatca tgggtgtagt gaatactttg tccaatatgg gcttttagca aggatgctga
    15721 acaaagaaat acaataaata aacagaattg gatttcttcg cttagcttct ttgctctagg
    15781 aatacattta aaggttgtga caaaattcgt gtcacaatct ttttttattt tgaataagtg
    15841 atgttcaaaa accattttct cggtattaag ccaaatttat gcaaaaatgt gagaaactat
    15901 tttcacaaaa tttttcttca tactcgaaac taatcggctt cttcacgacc tcttatacac
    15961 ggcgttcaaa agcttgcatc tgcgataata accccaacca aacgaaaaac atgagaagct
    16021 gtttatgttt ggctgttctt attacttgat gcaggacagc ttttttacga cctcttggta
    16081 cagggtagct tttttaccaa tttctagcta taattaaaga taaccttgaa agttgcagaa
    16141 aaaaagtgca aaataaacga taagtacaaa aagactagtt gaaaggacgt cgtcatgtca
    16201 gagcgtggat tattaatcgt gttgtctggt ccatccggtg taggaaaagg cacagtacga
    16261 aaagcaattt ttgagagtga ggataatgat tttcagtatt cgatctcaat gactacccgt
    16321 aaaatgcgtg aaggggaggt cgaaggcgtt gattatttct tccgaagcaa agaagagttt
    16381 gaatcaatga tcgaagctgg agagatgctg gaatacgcag aatatgtcgg gaattattat
    16441 ggaactcctt tgtcatatgt caacaagaca ttggatgaag gaaaagatgt tttcttggag
    16501 attgaagtcc aaggagcaaa acaagtcaaa gaaaaagtgc ccgatggtgt attcatcttt
    16561 ttgactccgc cagatttagc ggaattaaaa tcacggatcg taggccgagg aacagatgca
    16621 gatgaagtaa tcgaaaaacg aatgaaagtt gcaaaagaag aaatcgaaat gatggctctt
    16681 tacgactatg ctgttgtaaa tgatgaagtg ccgctagcag ttgaacgaat caaaaacatt
    16741 attgccagtg aacattttcg agtagatcgc gtaatcggta aatatattaa aatgttaaag
    16801 gagatgtagt cttatgatgt taaaaccttc aattgactca ctattagatc gtgtaaattc
    16861 aaaatattct ctagtgattt tagcaagtaa acgcgcgcat gaattggatg caagtgcaca
    16921 gcctactcta gattcttttg attcagtaaa aagcgtcggt caagctttag aagaaatcga
    16981 tgcaggaaat gtagtcaatg atccacatcc tgaattaaaa cgtgaacgtt tgaagatgga
    17041 agaagaggaa cgtcaagcac aaaaagagcg tgaacaacac gaattggaat cacgtatccg
    17101 tgaagagcaa aaaatgtaat aaagaaaaat agagcgaaca tctaaaaaga aaagcaaacc
    17161 cagctgtttc cttttcaaac attcgccact tattgtctgg tagaaaaggc tgtgtctgaa
    17221 ctttttgaca cagttctttt ttttcttatc tgctgttaat tttttgttag gatttggcag
    17281 aagataagac aaatccatcg atctctagta aaataggcaa ggaacttttt gtcgcttgat
    17341 aagaaaagac aaaaaaagga ggaaacgaac catgacatta acagctgaag tcattgtgga
    17401 cgtacctact atgcagaccg atcagccatt cacttatttg gttccctctg aaatggaaac
    17461 agctttgcaa gtcgggatgc gagtagaagt cccttttggt aatggcaatc gtcatgtcca
    17521 aggatttgtt atggctatcc aaaaatcaga agaatcagct aatccatctt tgaaggcagt
    17581 tattcgtttg ctagaccttg ctccggtggt caatgaagaa ttactttcat tagctgatta
    17641 tatgaaaaaa attacttacg cattcaaaat cacttgtcta caaacgatgc ttccgagtgt
    17701 gatgaaagca gaatatgata aattgatcta tcctttggca gacacaccag aagttcaggt
    17761 attgtttgat cagcgagaag tgatttcctg gaaggaagca gaagaagaag gcagcctttc
    17821 tcaattgatt cgctggcgcc aagagcaatt agtagatatc aaatatgaag tccatacacg
    17881 aaataaagta aaaacgattc gtttggtccg ttctttgtta actgaaaagc aaatcgaaga
    17941 agaatgggca aaattacggc aaaatgccaa gaaacaaaaa gaacttcttt tgtgtttatc
    18001 tgaactgagc caagaagaac caattgctta tttcaaaaat aaaggaatca gtactgctgt
    18061 cttgaatcaa ggaaaagaaa agggctggct ggagtttata gaatcagaac gttatcgcga
    18121 cccttataaa gaccgagtat ttgaccaaac gacggcattg gaactgaatg cagaacaaaa
    18181 aaatgctgtc gaacaaatca tcacagcagg tcagcagcaa aaagatcagg tctttttgct
    18241 ggagggaatc actggcagcg ggaaaacaga agtatatctt caggcaatcg ctgatgtttt
    18301 gtcagaaaat aaaacggcta ttatgttagt gcctgaaatc gcattgactc cgcaaatggt
    18361 cgagcgattc aaaggacgct tcggagaatc agttgcggtc ttacatagcg gactttccca
    18421 aggtgaaaaa tatgatgaat ggcgcaagat tgagcgggaa gaagcgcaag tagtagtagg
    18481 cgcacgatct gctatttttg ccccgctgaa aaatatcggg ctgatcatta tcgatgaaga
    18541 acatgaatca agttacaaac aagatgagac accgcgttat catgcgagag atctagcaat
    18601 ttggcgcagt aaatatcatc attgcccaat agttctagga agcgcgacgc cctctttaga
    18661 atcaagagca cgagctcaaa aaggcgttta tcagttattg cagttaaacc accgagccaa
    18721 agcagctgca cagcttccag ctattgagat cgtagatatg cgtgaagagt ttcagaatca
    18781 tcggacctct actttttctg ctaatttgca ggaaaaaata caaaatcgat tagacaaaaa
    18841 agaacaaact gtcctactgc tcaatcgtcg aggatattcg tcttttgtga tgtgtaggga
    18901 ctgtggattt gttcttccat gtcctaattg cgatatctct cttacactgc atatggatac
    18961 acgttcaatg cgctgccatt attgcggaca tgaagaacct attcccaatc gctgtcctaa
    19021 ttgtggcggc aataaaatcc gctactatgg aacaggtact caaaaagtag aagaggaact
    19081 ccgagaactt tatccgcaag ctaggatttt gcgtatggat gtagatacca ctcgaagaaa
    19141 aggggcacac gaacagattt tacaaaaatt cggtgcaaaa gaagcagata tattattagg
    19201 gacacaaatg attgctaaag gtttggactt tccagaagtg acattagttg gggtgttgaa
    19261 tgctgatacc tccctgaatt taccggattt tcgttctagt gagcatacat tccaattgct
    19321 tacccaagtt tcaggacgag cagggcgagc ggaaaaagca ggcgaagtcg tgatccaaac
    19381 attcaatcct caacattacg ccatagaatt ggctaaaaaa caaaattatg agcagtttta
    19441 tcagcaggaa atgcatgtac ggcatcgtgg aggctatcct ccttattact ttactgtgaa
    19501 aataaccgcc agccatcctg aagaacaagt agcggcaaag aaaatctttc agattgccaa
    19561 tcagctaaaa gaagtgttaa gccctcaaag cttgctatta gggccaacgc ccagcatgat
    19621 tttgagagtg aaaaaccgat attattatca actaatcatc aaatataaac atgaaccgaa
    19681 tcttcctcat gttttggatg aaattttgaa tggaagccag aaagaacagc gacaaggtct
    19741 ttttgtggca attgacaacg aaccattaaa ctttatttaa gaaagggcca aattatgcgc
    19801 tatcctatat tgatccaccc taatgataaa ttaaaaagaa ctgctcagcc aatcgatgta
    19861 atcacggacg agacaatcgc tttattagac aatttatatg agacaatgat tgcgaatgac
    19921 ggaataggga tcgctgctcc tcaagtagga caaaacaaga gaattgctgt gatcgaagta
    19981 gatgaaggcg agaaatttga actgattaac ccagaaatca tcgaagcaaa aggtgaaagc
    20041 cttgacgtag aaggttgtct cagtattccg catgtctatg gaacggtcaa gcgagcagat
    20101 gaagtgaccg tgcgttatta tgatcgagac ggagaagaga tagaagtgac ggcttttggc
    20161 tatttagccc gtgccttcca gcatgaaatc gatcacttag acggtatctt gttcattgag
    20221 aaaatgatac agcagatccc ggaagaagaa cttgaagaat atatggagga acatcttgat
    20281 gactaagcta gtatttatgg gcacacctgc tttttctgtg ccgattcttg aagggctgat
    20341 agaatcaggt tacgagatcc aggcagtggt aacacagccc gaccgtccag ttgggcggaa
    20401 acgagtaatt acgccgacac cggtaaaaaa agcggctctg aagcacggta tccgtgtgct
    20461 gcagccagag aaaatctccg gttcgccaga aatggaagaa atcattgaat tggtgcctga
    20521 tgtgatcgtc actgcggcat ttggccaatt cttgcctgaa aagttattgc aagtaccaaa
    20581 attaggggca atcaatgtcc atgcttcttt acttcctaaa tatcggggag gtgcgcctgt
    20641 tcattatgcc atcatgaatg gggagaaaga aactggtgta acgatcatgg aaatgatcaa
    20701 aaaaatggat gcaggcggaa tctatgcgca agaaagcata ccaatcacta aacaagatga
    20761 tgtagggacg atgtttgaaa agcttagcct tttaggaaga aagctattgc tagaaacatt
    20821 gcccaatatt ttggatggac aaaaaccagt acctcaagac gagtcagaag caactttttc
    20881 gccaaacatc acacgggaac aagaagcaat cgattggcat aaaactgcag aagaaattga
    20941 caaccaagta cgcggaatgc gtccttggcc aattgcattc acgacctatg agggcatacg
    21001 ctggaaatta ttatcggttg aagtattggc agaaactaca gacaaagcac ctggtacgat
    21061 catccggaaa gacaaaaaaa atctatggat tgcttgcggt aaacagaccg tcttagccat
    21121 caatgaatta cagccagctg gtaaaggaaa acaggccgta catgatttcc ttaacggaag
    21181 cggtcagcaa gttcagattg gtcagcaggt gaaataatga aaaaaatccc taaaaaaatg
    21241 aaaaaatcag tacgatatat ggcattactg acattagagc gagtgaacaa gggtggcgcc
    21301 tactcgaatc ttttgttgaa tgagatgatc aaacaaagcg agctaaatca aaaagatgtg
    21361 ggtttattta cagaattgat ctatggtacg attagccatc agcgactatt ggaattttac
    21421 gttgcaccat tgatcgctaa agcaaaaaaa gtggatgact gggtcaagat gcttctttat
    21481 ctttctgtat accaattaga atttttagat aaagtccctg cacatgctat tttaaacgag
    21541 gcggtcgaaa tcgcaaaagt aaaaggaaat cctgggacag ggaaatttgt aaatggtgtc
    21601 ttgaggaatt atcagcgtca aggagcacca gatctgcaag gcattgctga tcctatcgaa
    21661 agactttctg tcaaaatcag cctgcctcta tggatgacga aacgactggt ggatcaaatc
    21721 ggatacgaag aaactgaaaa gctcggttta tcgctatatc agcctagcca tgctagtgcc
    21781 cgagtagata cacgccgact ttccagagat caggccatca ctgttttgca agaagaagag
    21841 atcacagctg aaaaaagcca agtcagtcca tacggtatag tagcgaaaaa agggcattta
    21901 gcttctagct cattgttcca cgatggcgtc atgacgatcc aagatgaaag ttcgatgctt
    21961 gtagcgccgt ctatgcaaat cgagcctgaa catcatgtct tagatgcctg tgcagcaccc
    22021 ggaggtaaga cgacgcatat tgcttctttt ttagatccgt catgcggtgg agaagtggtc
    22081 gctttagatg tacacgaaca caaagtcaaa ctcattgaag ataatgcagc acgtctaggt
    22141 gtctcttctg ctgtctacgc tcaaaagatg gatgctagag aagtcagtaa acagttcgaa
    22201 gcagagcagt ttgatcggat tttagtagac gcgccttgtt caggtcttgg gctgcttcgg
    22261 cgaaaaccag atattcgtta taaaaaacag ccggatgaat tagcgaattt accaaaaatc
    22321 caattggaaa ttttagaaag ctgcgcacca actttaaagt cttccggtat tctgacttat
    22381 agcacttgta cgatcttaaa agaagaaaac caagacgttg tttctgcttt cttggaaaga
    22441 caccctgatt ttgagaggat tgatgttttg accaatccta tgctacaatc atcaatcgaa
    22501 gagaaaatgt tgactttgta tccgcatcag ttttacacag atggattttt catttgttgt
    22561 ttgcgcaaaa agtaatgagg tgaaaaaatg cagattgaat atcaatcaga tgtaggaaga
    22621 cgacgtaaca caaatcaaga ctatgccagt gtatttacca gccgttcagg tctcaagctt
    22681 gctgtattag cagacgggat gggtggtcat cgtgccggag acattgccag tcagatggcg
    22741 gtaacgaatc taggcgaagc ttgggaaaaa caagaagctg ttgacgatga aaaaatcgcc
    22801 caatggttca ttcaatcgat tcaagaagaa aatatgctga tttatcagcg tggacaagaa
    22861 cagccggaat ataacggtat gggaacaaca atcgtagcgg ccgctctgtc agatgactgg
    22921 ttcacgattg ccaatgttgg ggatagcaga gcttatttga ttcgtgaaga taaaatcatg
    22981 caattgacgg aggatcattc tttagtcaat gaactagtca aatcaggaga aatcagcgaa
    23041 gaaatggcag tgaaccatcc aaggaaaaat attttaacac gttctgtcgg catgcctgga
    23101 acagtcgaag tagatgtttc tactcatctt tggcaactaa aagatcgact gctgttatgt
    23161 tcagatggct tgacgaacat gctgtctgaa gaagaaatag gaacgatcat caatcaagaa
    23221 ggcccattgt ctcataaagt aactgagctg attgatcgtg ccaatgaagc aggcggagcg
    23281 gataatatca ccgttcttct tatcgaatat aaggaggaca gtgtatgatc gagttgggca
    23341 aaaaattaaa tggccgttat caaatcactg gaaatatcgg tagcggtggt atggcaaatg
    23401 tatttttggc gcacgattta atcttggatc gtgatgtagc agttaaagtc ttacgatttg
    23461 actttcaaaa tgaccagact gcgattcgac gatttcagag agaagcttta gcagcaacag
    23521 aactcgttca tccaaacatt gttagtgttt acgatgttgg tgaagaagac aacatgcagt
    23581 atctggtgat ggaatacgtc aaaggaatgg acttgaaacg atatatccaa acacattatc
    23641 ctgttcctta tgaaacagca gtaaatatta tgcagcagat cctttctgct atttctttag
    23701 ctcataacca tcaaatcatc caccgagatt tgaaacccca aaacgtactg atcgataatg
    23761 aaggtgttgt aaaaatcacg gatttcggga ttgctatcgc tttatctgaa acatcgatca
    23821 cgcagacgaa tacgatgcta ggatctgttc attatctgtc ccctgagcag gccagaggaa
    23881 gtatggcaac gaagcagtca gatatttatg ctttagggat catcctttat gagatgttga
    23941 cgggtagcgt accgtttgat ggtgaatctg ctgtaacaat tgctttaaaa cattttcaag
    24001 atgatcttcc atctataaaa gcacttgatc caaatgtgcc acaagcactg gaaaacgtga
    24061 ttttacgggc aacggctaaa gaacctgcag accgttataa gtcagccgag gaaatgtctg
    24121 atgatctttc tacagtatta tcaccagctc gtgcaaacga agaaaaatgg cagcctcatg
    24181 tgatggataa tgaaacaaaa gttatcacac cccttacgga agatgcacca atgcctgatt
    24241 cattcaaatc tatgcctctg ccaaaagaca agccagaaaa cgaaacctta ccagaggagg
    24301 aagaagttct gcctgatcct aaaaagaaag gacagaaaaa atggtggctg atcctattag
    24361 ctattcttgc tgttttagga atcggaacag ctgtattctt tgcttctggc gggcgaggag
    24421 aagttgttgt tcctgatgtt tctgatcttt ctgaagcttc tgctagagaa acactcgcaa
    24481 aagcgaaatt agaagtagca acaaaaacag aagaaattgc agatgacaaa atcgaagaag
    24541 gtaatgtagt gaagacagac ccggcagcgg gaacgacagt caagcaaaaa cgcgaagtca
    24601 ctctgtatat tagtagcggc acgaaaaaaa tcaaattaga tgattacagt ggtaaaagtt
    24661 atgaagatgc cgaagcaagt ttgaaagaat tagggttttc tgctgacttg attcaaaaag
    24721 aagaggaatt cagttcagag gtagaaagcg gaatgatcat ttcgcaatct ccatcagaag
    24781 atacagaagt cgatcctaaa acagacacga ttactttcac agtcagcaaa ggaccagaaa
    24841 cagtgacttt gagtagtcat gtaggagaaa actacgaaac tgttcgtaat tggcttgtta
    24901 cacaaggatt caatatcaat aatattagtg ctacttatga ctatagcgac actgtaccat
    24961 caggttcgat catcagtcag gatccagcct cgggtgaagt tgtagcagaa gaaacttaca
    25021 tcaattttgt cgtaagccaa ggaacagagc ctaaattgaa tgatatctcc ggctatacaa
    25081 aaagcgaagc acaaagttat ttagctagtg tcggtgcaga atatatcggg catgagactt
    25141 ccgaatattc aaacacggtg gataaagata aagtgattcg tacagtacca ggggcaggaa
    25201 cgacaattac caaaggaatg gttgtgaacg taatttactc taaaggacca gatccagcgt
    25261 cttctagttc tgaaaaagac tcttcagaca gttctagcag ctcaagtagc tccagtagca
    25321 caagcaactc ttcaaaagaa gaatcttcca gctccacaag ttcatcagct agcacctcag
    25381 aaagttcaaa ataagtgctg aataaaatat atcagaaggg aatgcgacat tgatttgttg
    25441 cattctcttt tttgtgtttc tatctctttt gatcaatttt caagccatat actttgagaa
    25501 gtttgcatgg aataacacct actttcctct tcgtatggta aaataaacaa aaacggaggt
    25561 ggcacttatc aaaggacaaa tcagaaaagc aatcagcggt ttttactatg tatacgcaaa
    25621 cggagagacc tatcaaacaa gaggaagagg aaatttccgg aatcggaaaa tcacgccgct
    25681 tgtcggagat caggtgatct ttgagagtga aaatcaaaca gacggctatc ttctggaaat
    25741 cttgccgaga aaaaatcaat tagttcgccc accagtcaca aatgttgatc ttggaatcat
    25801 tgtcacaagt atcgtagagc ctgcattttc ttataatcta cttgaccgct ttctagttac
    25861 cttggaatac gagtcgatcg aaccgattat ttttttgaca aagaccgact tgtcagacga
    25921 cccagaaaaa atcgaagaga tcaagcaaac ctatactaaa ataggctatc cggtgattgt
    25981 acccaatcat acgggtgata cgacagaact gatccgttac ttcccagaac ggctatcggt
    26041 ctttatgggt cagtctggag caggaaaatc gacgctactg aatcaaatat caccggaact
    26101 gaatttagca acaggagaga tttctgatgc acttggaaga ggacggcata caacacgtca
    26161 tgtggaactg attcctatct atggcgggtt agtagcagat acaccaggat ttagctcgat
    26221 tgactttttg acaattgaag caggagaact gccgagacaa tttcctgaat ttgtagaagc
    26281 ttctgtccat tgcaaattta gagagtgcat gcatgctaaa gaacctggct gcgaagtaaa
    26341 acatagagta gaaacaggtg gtatcgcaca gacacgttac gacaattatt tgcaattctt
    26401 acaagaaatc gaaaaaagaa aaccaatgta tgcaaaaaaa gaaggtaaac gaaaatagga
    26461 ggaaacaaga atgaagatag caccatccat tttgagtgca gattttgcca atttacaaag
    26521 agacattgag ttagtggaaa aaggaggcgc agattacatc cacgtagatg taatggatgg
    26581 ccgatttgtc ccaaatatca cattagggcc aaatattgtt caggcaatcc gtcctgttac
    26641 taaattaccg ttagacgtcc atctgatgat cgttgatcca gaaaactaca ttccagcatt
    26701 tgcaaaagct ggagcagata tcattactgt ccacgtcgaa gcaactcctc atattcatcg
    26761 ggcacttcaa atgacaaaaa gcttaggagt taaagctgga gtagttatca atccaggaac
    26821 accagtctcc atgatcaaac atgttttgcc tttggctgat caagtactag tcatgactgt
    26881 caatcctggg ttcggcggac aaagctttat tgaagaaaca gtagaaaaaa ttacggaact
    26941 cgctgatcta cgtagagaga acaattggac ttatgcaatc gaagtagatg gcggaatcgt
    27001 tccagaaaca gcaaaaatct gtaaagatgc aggggcggat gtcttcgttg caggatcata
    27061 tatctatgga gccgaaaatc cagtcggaca aatcgaacga ttaaaagagg cattagacta
    27121 aatgaaaatt ttattagctg ctggaggtaa tccgcaaaac tggccgcagt ctctgctgtc
    27181 caaatacgat gcctatgtcg ggatagaccg tggtgcgctg tacctgcttc aagcaggcta
    27241 cccagtagat ctggcagtgg gtgattttga ttcattgtcg aacgaagagc agaaggaagt
    27301 atttgcaaaa gccaaagagt atacccgttc ccctgcagaa aaagacgata cagatacaca
    27361 gttaggtatc ttaaaaacat ttgaacgtta cccgcaagcg gaagtcactt tgattggagc
    27421 tacgggcgga agattggatc atcttttagc caatctctgg ttaggtatgg aacctcgctt
    27481 caaacccttt gcccatcagc ttattatcag agattgtcaa aattatttgt cttattattt
    27541 gcctggagag catatagtaa gacagatacc agatatgaag tatttagctt attgctgctt
    27601 gactcccgtc tcgaatctta cattaaaagg cagcaagtat gtattggaaa ataaagaagt
    27661 agaatatccg acatcttatg caagtaacga atttctttca gataccgcag aattttcttt
    27721 ttcagatggt atgattgcag ttattcaaag caaagattga tccataaaca aataaaaaaa
    27781 tttgtctttc aggctaaaaa tgacagaatc tattatttgc taaaaaaaag cttaagagga
    27841 gcaacttacc ttttgtttca cggcatttag tgataaaatt gaacacaaga gtctaattaa
    27901 ttcgaggtgt ctttgtgaag aagttcaatc ctaataaaaa tattattatt accttggtta
    27961 ttgttatcat cgtggtgacg atactaagtg tgaccgctgc gcagcgagcg aatgaaggaa
    28021 agcccaattt cttccaatcc atcgttaatg acagtgttgc aatggttgat aaaacaatct
    28081 ctgccccagt acggtgggta cgaaatggag tagattctgt ccataatctt ttttcaactt
    28141 atgccgaaaa tgagcggcta aaagaaaaaa tcgatagcta tgatgacgtt tcacagcaaa
    28201 acaagaatta cgagagagaa atcgaagcgt tgaaaaaaga acttgagttg aatcagacgc
    28261 tgactagtta cgaaaaagta acggcaaatg tcattactcg ttcgcctgac agttggcagg
    28321 acatgctgat cgtagacaag ggttctaaag acggtatcga agcaaatatg gcagtcatgt
    28381 cgcaaaaagg attgattggc cgagtgatcg aggtcaatac ggcttcgtct aaaatcgaat
    28441 tactgtcatc ctctaatgaa agctccaatc attttccagt acgggtatct tcggctaatg
    28501 gcgaagcgtt tggtttgctt aaaaactatg atgaaaagct ccatgcctta gtggtgaccc
    28561 aattaactgg tgatacggat atcaaagaag gggatgttgt ccagacatcc ggtcttggag
    28621 ggaattctcc agctaacttg ccgatcggta cggttattaa aacgaaacca gatagttatg
    28681 ggctggatcg ggaagtttat gtgaaacctt atgcagaaat gtatgacgtg tcagttgtga
    28741 cgattgtcca acggctagcg gaggattagt ccatgttgaa gaaagaaaca atgaagtact
    28801 atctgccaat cgttttgttc tttttgatgt tgatagatgg tcatttaaca agaatgctag
    28861 gggagtggtc gaaaggcacc tatatgtcaa atgcccactt tctgatattg gcattattat
    28921 gttgcagtat ggcgtttgaa aaacgttatt tactgattac cacgattgtt ctcggggcta
    28981 tctatgacgc ttactatatt ggcgttatcg gtatctatgc agtagctctc cctttaattg
    29041 tatggttgat gtatgtaatg aaagacgtta tccatgtcaa catctttact gaatttttca
    29101 gtatgatcat ctttgtcacg ggttatgaat tgtttacgat ggtggtccag ttgattttta
    29161 aattagcagt agtaaataac acgtatttta ttacaaggtt tttaggacct acactgctgt
    29221 tgaacatgat tatatttgta ttattcattt ttccctttaa gaaattattc agtgatgaat
    29281 aatttctttt tttattttta ggtcgaaagc gaaaaagaat cgttgttatc ttaagaaatg
    29341 ctagacttaa cgcggctttt tcatgatcat tttctgaaac gaacagtttt gtcattttta
    29401 aaagttgttt tgaaacattt ttgtaacata actattatat cgttgacaca ctgctatggt
    29461 acaattttta tatcgttaag aaagacttaa atatatttag atatttatta aattaagcca
    29521 gtcggaggaa tgaagagtga aaaagagttt aatatcagca gtaatggtat gttcaatgac
    29581 cttgactgcc gtagcatcac cgatcgctgc cgcggcggac gattttgatt ctcagataca
    29641 acagcaagat caaaagattg cagacttgaa aaatcaacaa gcagatgcac aatcacaaat
    29701 cgatgcctta gaaagccaag tttctgaaat caacacacaa gcacaagacc tattagcaaa
    29761 acaagataca ttgcgtcaag aatctgcaca attagtaaaa gacattgcag atttacaaga
    29821 acgtattgaa aaacgtgaag atacaatcca aaaacaagca cgcgaagcac aagtaagcaa
    29881 cacaagctca aactacatcg atgctgtatt gaatgctgat tcattagcag atgctattgg
    29941 acgcgttcaa gcaatgacaa cgatggtaaa agcaaacaac gacttgatgg aacaacaaaa
    30001 acaagataaa aaagctgtag aagacaaaaa agctgaaaac gatgctaaac taaaagaatt
    30061 agctgaaaac caagctgctt tggaatctca aaaaggcgat cttttatcta aacaagctga
    30121 tttgaacgta ctgaaaactt cattagctgc tgaacaagca actgctgaag acaagaaagc
    30181 tgacttgaac cgtcaaaaag cagaagctga agcagagcaa gctcgtattc gtgaacaaca
    30241 acgtctagca gaacaagctc gtcaacaagc tgcacaagaa aaagctgaaa aagaagcacg
    30301 cgaacaagca gaggctgaag cacaagctac tcaagcttca tctactgcac aaagctcagc
    30361 aacagaagaa tcatcagcta cacaatcttc aatgactgaa gaatcatcat cagctacaca
    30421 atcttcagca a
//
LOCUS       DACDYI010000035        30259 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.035, whole genome shotgun sequence.
ACCESSION   DACDYI010000035 DACDYI010000000
VERSION     DACDYI010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.035"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            136..1455
                     /locus_tag="IWM52_001468"
     CDS             136..1455
                     /locus_tag="IWM52_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HAP9973725.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            1896..3536
                     /locus_tag="IWM52_001469"
     CDS             1896..3536
                     /locus_tag="IWM52_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAP9973726.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            3690..5012
                     /locus_tag="IWM52_001470"
     CDS             3690..5012
                     /locus_tag="IWM52_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAP9973727.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(5074..5505)
                     /locus_tag="IWM52_001471"
     CDS             complement(5074..5505)
                     /locus_tag="IWM52_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9973728.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(5569..6405)
                     /locus_tag="IWM52_001472"
     CDS             complement(5569..6405)
                     /locus_tag="IWM52_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAP9973729.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(6595..6897)
                     /locus_tag="IWM52_001473"
     CDS             complement(6595..6897)
                     /locus_tag="IWM52_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAP9973730.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(6923..7354)
                     /gene="ruvX"
                     /locus_tag="IWM52_001474"
     CDS             complement(6923..7354)
                     /gene="ruvX"
                     /locus_tag="IWM52_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAP9973731.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(7351..7617)
                     /locus_tag="IWM52_001475"
     CDS             complement(7351..7617)
                     /locus_tag="IWM52_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAP9973732.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(7871..8596)
                     /locus_tag="IWM52_001476"
     CDS             complement(7871..8596)
                     /locus_tag="IWM52_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAP9973733.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(8962..9032)
                     /locus_tag="IWM52_001477"
     tRNA            complement(8962..9032)
                     /locus_tag="IWM52_001477"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8998..9000),aa:Gly,seq:ccc)
     gene            9149..9901
                     /locus_tag="IWM52_001478"
     CDS             9149..9901
                     /locus_tag="IWM52_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAP9973734.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            9917..10396
                     /locus_tag="IWM52_001479"
     CDS             9917..10396
                     /locus_tag="IWM52_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAP9973735.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(10647..12227)
                     /locus_tag="IWM52_001480"
     CDS             complement(10647..12227)
                     /locus_tag="IWM52_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAP9973736.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(12220..12963)
                     /locus_tag="IWM52_001481"
     CDS             complement(12220..12963)
                     /locus_tag="IWM52_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9973737.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(13139..13915)
                     /locus_tag="IWM52_001482"
     CDS             complement(13139..13915)
                     /locus_tag="IWM52_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9973738.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(14163..14999)
                     /locus_tag="IWM52_001483"
     CDS             complement(14163..14999)
                     /locus_tag="IWM52_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAP9973739.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(15010..15939)
                     /locus_tag="IWM52_001484"
     CDS             complement(15010..15939)
                     /locus_tag="IWM52_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP9973740.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            16131..16484
                     /locus_tag="IWM52_001485"
     CDS             16131..16484
                     /locus_tag="IWM52_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP9973741.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(16558..17214)
                     /locus_tag="IWM52_001486"
     CDS             complement(16558..17214)
                     /locus_tag="IWM52_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP9973742.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            17440..19374
                     /locus_tag="IWM52_001487"
     CDS             17440..19374
                     /locus_tag="IWM52_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9973743.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(19460..20683)
                     /locus_tag="IWM52_001488"
     CDS             complement(19460..20683)
                     /locus_tag="IWM52_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAP9973744.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(20677..22575)
                     /locus_tag="IWM52_001489"
     CDS             complement(20677..22575)
                     /locus_tag="IWM52_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAP9973745.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(22569..23687)
                     /locus_tag="IWM52_001490"
     CDS             complement(22569..23687)
                     /locus_tag="IWM52_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP9973746.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(23770..24603)
                     /locus_tag="IWM52_001491"
     CDS             complement(23770..24603)
                     /locus_tag="IWM52_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9973747.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(24596..25513)
                     /locus_tag="IWM52_001492"
     CDS             complement(24596..25513)
                     /locus_tag="IWM52_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9973748.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(25740..26864)
                     /locus_tag="IWM52_001493"
     CDS             complement(25740..26864)
                     /locus_tag="IWM52_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9973749.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            27047..27955
                     /locus_tag="IWM52_001494"
     CDS             27047..27955
                     /locus_tag="IWM52_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9973750.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            28096..29709
                     /locus_tag="IWM52_001495"
     CDS             28096..29709
                     /locus_tag="IWM52_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAP9973751.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         9425 a         5948 c         5173 g         9713 t
ORIGIN      
        1 aattaaaacc atgtgaaact gcctcacggc agcttacaat tactttttca tcttcacccg
       61 ttaagtgatg aaaaaagaac ccctttctgc tatggttaaa gtgactaaac caaccaaaag
      121 aaaggagttc ttttcatgac tagcttacac aaaaaccaag taaaattcaa ttcaaatatc
      181 actatttctc atacaggtgg tcaattatcg agtgattcgg gtctcgtcct agtgaaagag
      241 ctgatgaaca tctttggttt ttctgaactg gctaagcaac acattcacat tgaagacgaa
      301 cgagcgtatt ttactcatga caacttatcc atacttgagc agttcattat gcaattaatt
      361 gctggttact cagctgattc tgcagctaat ctcctgagac aagatcctgt gtttaaagct
      421 gttttagata ggaaagaact cgcttctcaa tcttcacttt cccgattttt agatcgacta
      481 tctgaggaga atatccatga acttcaagct ttgaaccaag aacttatcga taaagcacgc
      541 ctcatccgta atgatacgga attaatcatt gatttagatt cgacccattc agatacattt
      601 ggtcaccaag aacaaacgaa ttataataca cactaccaaa cctatggtta ccatccattg
      661 gtagcttttg acggattgac tggtgacttc ttaaaagctg aactacgttc aggaaatcaa
      721 tatacatcaa aaggcgtaaa ggagtttctc acacctttat tagagcacta taatcactct
      781 ctaccaaaca ctgacatctt ggttcgtgga gacagcgggt tcgctacacc tggtgtgtat
      841 gattcgtgtg aatcaaaaaa gagtcattat gttattcgac tgaagaataa tcgtagacta
      901 ggtcaaatag ctgagaaatc agtactttat ggcgataatc aaaagtggga agaacgagaa
      961 gttcagtact tctcgaccac ttatcaagca caatcctggt cacaaagtcg tcgcgtgtgt
     1021 atacgctcaa cacgtgaagc gggcgaacta ctctttcgac atgaatttat cgtgacgaat
     1081 ctatcagaaa atgtttctcc tgataccatc ttttctatct atgccaaacg tggcacaatg
     1141 gagaacttca ttaaagaagc gaaagctggc ttttactttg acaaaacaga cagtcctcgc
     1201 tttttggaga atcatgtccg aatgatgctc agcttgatag cttacaactt agtcaacttc
     1261 ttaagaacga ttggctttga agaagtccaa aagggaatga ccattcattc tattcgattg
     1321 aagtttctga aagttgctgg gaaattagtc caaacgggta gacgagtcta tctcaaatta
     1381 tctagttatc atgtgtatca gaatgaattc tacagggtct ttgctcgcct gaggcgagcc
     1441 agtcaatgga tctaatccaa caatctaacg atttttttac tgggaagtta tccagggaga
     1501 agtatgctca aaatgcctta cacccaaaat ttattcctca atatttttga acagtgaatg
     1561 tttgaagtaa ctttttagtc aaaatcagtt gatagggata ttagtgatac atatttcaat
     1621 aaaatgtgcc aaagaattaa agctatgaat tattcaggat tctatttatc tatctaacat
     1681 atcctttatt cttctacttt tctttcttta gcatataaga gatacgcata gtaatcaagc
     1741 aaggattgtt gatataaaaa gaacaaatac cgctattttg atttcttttg actctattac
     1801 tcatataaat aaaatttatt atacctgctc tttttgacta tatcctcatc tgactcctat
     1861 acttcgtttg tattaaaact taggaggaat cattcatgct tacagggcta aacttattaa
     1921 ataatccttt tctaaataaa ggaacagctt ttacaaaaga agaacgggca aaatacggaa
     1981 tcactggtat gcttccaagt actgtccaaa cattggaaca acaatctgtc caagcttacg
     2041 gtcaatactt aagcaaacag accgatttgg aaaaacgtat cttcttaatg aatctattca
     2101 atacgaaccg gacattgttt tataaattga tgggcgaaca tttagttgaa ttcatgcctg
     2161 tcgtttatga tcctgtcgtt gcggattcaa tcgaacaata caacgaaatt ttcttgaaac
     2221 cacaagatgc tgtatttctt tcaatcgacg aaccagaaaa tatcaaagca agtttgaaaa
     2281 acgcagcaga tggacgcgac attcgtttga tcgttgtgac agatgcagaa ggaatcttag
     2341 gtatgggtga ctggggtgtg aacggtgttg acatcgctat cggtaaattg atggtctaca
     2401 ctgccgcggc tggtatcaat cctgcacaag tcttgcctgt atcaatcgac gctggtacta
     2461 ataacgaaga attattgaat aatccattat acttagggaa tcgtcatgct cgtgtagaag
     2521 gcgaaactta ctacgaattc attgatcaat tcgttcaatc agctactgaa ttattcccag
     2581 aattgttatt acattgggaa gattttggac gaggaaatgc tgcaacgatc ttagaaaaat
     2641 acgaagataa aatcactact ttcaatgatg atatccaagg cacaggaatc gttgtactag
     2701 ctggtgtatt aggtggattg aatatatcag gtgagcaatt gaaagatcaa acgatcttga
     2761 catttggtgc tggtactgct ggtgtaggta tcgcaaacat ccttttagat gaaatgatcc
     2821 gtcaaggtgt acctgaagaa gaagcacgcc aacatttcta tcaagtagat aaacagggtt
     2881 tattgtttga agatacaaaa ggattgactc ctggtcaaat cccattcgct cgtaaacgct
     2941 cagaattcac taatgctgat gaattgacaa acttagaagc agttgtaaaa gcaatccatc
     3001 caacgatcat gatcggtact tctacccaac ctggagcatt cactgaagaa atcgtgaaag
     3061 aaatggcggc tcatacccca cgtccaatca tcatgccttt gtctaatcca acaaaattgg
     3121 cagaagcaaa agcaaaagat ttgattgaat ggacggacgg aaaagcttta gttggaacag
     3181 gtatccctgc tgctgatgtg gaatataacg gtgtaactta ccaaatcggt caagcaaata
     3241 atgcgttgat gtacccaggt ttagggcttg gtttgattgc ttcaacagct acacgagtaa
     3301 atgctgaaat catttctcaa gctagtcgag cattaggcgg aatcgttgat gtaacaaaac
     3361 caggagcagc tatcttgcct ccagtagcaa aaatcactga attctctcaa acgattgctg
     3421 aaactgtcgc aaaatcagtt gttgcacaaa aattgaatcg tgaagaaatc acggatatca
     3481 aagaagctgt tgaatctgct aaatgggttc ctgaatataa atctttagaa gattaagatt
     3541 gttatagtaa aagaacatac agaaactttg tatgacgtct aaaaagaaaa aagcgactgc
     3601 ggcaaaacag cagccagcca actactgctc ttttgccgct tctctttggc ttttttcata
     3661 cgatacagaa taagaaagga tctgtaaaaa tggcacaaaa aacaacaacc gcaactgcag
     3721 ttgaggaaaa gaaaaaagaa gggttttggt caacaaaaat taccggcgta agtttgcccc
     3781 tttatttgat catggtcctt gtcctgatca ttgcaatggc tttaggaaaa ttaccgacga
     3841 atatgattgg cccaatcgct gttttggtca ttttcggtaa tttatttcat tttatcggaa
     3901 caaaaatccc tatagtcaaa agttatttag gtggtggctc cgtttttgcg atctttgcct
     3961 ctgccgcgat tgctacattt gggatcttac ctgagtatgt agtaaaatcc acagaaaact
     4021 ttgtcagcaa tatgggcttt ttagactttt atattgctgc cttgattact ggtagcatcc
     4081 ttggtatgaa ccgtaatcta ttgatgaaag cttctgttcg ttttattccc gttgcactta
     4141 tttcaatggt cgtctccttt tttgccgtag gactcgtagg tatgcttatc ggaaatggtt
     4201 ttgcaaattc cgttttatat atctcattgc ccgcaatggc tggtggtgtt ggagccggag
     4261 ctgtaccttt atcaacgatt tatgcaaatg ttttaggtac ggacgcttct tctatcattt
     4321 cccgcctgat tcctgcgaca gcaatgacaa atgtcctagc tatcattggt gcagcgttag
     4381 ttgctcgtgc gggacaatca atgccaaaat tcaacggtaa tggtaaatta atggaaaaag
     4441 gcgaccttgg cgatggaaaa cctagaaatg taaaaccttc cattccacaa ttaggtgtcg
     4501 gtttgatggt atcattgact ttcttcatct taggacaaat ctgtaactat tttgttccta
     4561 aagtccatgc ttacgcattc atgatcatta tcgttgttat ctgtaaaatc gcaaacctat
     4621 taccagaata ttacgaagat gcagctatca tgttcaacaa cttgattgtc aaaaacttga
     4681 cagcagctgt tcttgccggt atcggtattg cgttattgaa tctaaatgta ttggcttctg
     4741 ctttgacatg gcaatttgtt gtcttatgtt tgacaagtgt tatcgcgatt tcagtagttt
     4801 caggatttgt cggtcgtttg tttggtttat atccaatcga atcaactatt actgctggct
     4861 tatgcaacaa ctcgatgggt ggtacaggaa atgtagctgt cttatctgca gctaatcgga
     4921 tggaacttat tgcttttgct caaatgggca atcggcttgg tggtgccatc gttttgatca
     4981 tctcaggttt cttgatgcaa ttactttctt agtcaaaaaa aatacctgat catcaagtat
     5041 cggcttgatg accaggtatt ttttgataaa aatttacagg attttcaaaa atcttttttg
     5101 cattttatcg gaatgataac cgatgcgttc ataaaatcgg tgtgcttcta ctctgtattc
     5161 tgcagaatta agtcttattg ctgatattcc tcgtcttttc gcttcttcta cgccagtcat
     5221 caaggccttt ccgattcctc ttttttcagc atgtttagac acagcaagtg ccataatatt
     5281 gaacatcgga tcgctgaaaa tcgattcata ggactccgct tgtacatagc cagctgcttt
     5341 tttagaagta ggatcttcat agaccaagaa aaaatttttt tctggctcct tcaacagttt
     5401 cttcatttgt ctcaccgtaa cggttaatgg tacatcatat cctaattgtt ccgcattgat
     5461 ttctcgtagt gcttctgcat ctcctaaacg catttcccgc aacatccaaa tcaccagcca
     5521 atttttattt ttttaaatga atttttttat cacagtatac tccttttttc acttaaaaaa
     5581 agacttttca gtgattttga cttgcttgaa tccagatcct tctaatgttt gcaagatgac
     5641 gaaagcattg taaagattga tcaaaagaac cacgatgaca atcgcttgaa aaataaaggt
     5701 cacttttttt ggagacaaaa ccctgctaaa tcttgctccc cacaaacctc caataacagc
     5761 agcggggata acataaaaca acatagataa atcaaagcgc ataaagctac ttgttaaagc
     5821 aatcgttacc aattttgcca gttgagaaaa gaaaatcgta gatagagaat atagtgttgc
     5881 ttcttttatt ggcaatgcaa acatcagcat caacaaagaa acattgactg gtccaccacc
     5941 aatacctaaa aaactagcta aaaagcccag aaccccccca catataaggt accaagtcca
     6001 tgacgtcaaa tgaaattttg gctgatgatg cttggtgtag aagaacgcaa aaaccaaggt
     6061 caataccgtc aaaaaaattt ggatcagctg cacatgcttc tcgttttcaa ataactgtaa
     6121 aaaaacttcg aatacaatgt tgccgactac tcctccaaca acagctccac tagataccca
     6181 accgacaatc tgccaattca gtgatttccc gcttgatact tgtcgcacag tcgaaacaat
     6241 ggacatcgtg aataccgcaa ctgttgagta aaaagaaatc cctgcaacag aatgggcgcc
     6301 gataagatca agaatcggtt tgatcaacac gcctccaccc atcccggaaa tagcgccaac
     6361 tgtgttcgcc agaacgatca ccacaaaata aatcagacca atcatcttgt atttcctttc
     6421 tcttgtttat atcttttagc gattgttact cttttctttt attatacagt aaaagaaaca
     6481 aacgaggctt cgaaagcttt tctacttttt aaggaaattt agtgctaaac gaaaaaaccg
     6541 ggctctactt ttcagagagt ccagcttttc ttacacctta tcgattcttt tgttctattc
     6601 ttcttccgcc atgaatgtat tgaatacttc ttcgatcata tcccattctg cgtctgtttc
     6661 gatttgttct aactcgcctt ctgttccatc ttcgttttcg atgtatgcat aagcttgtaa
     6721 ttctacatct tcatcttctg aaacaccagc tggatataaa agaacatagt tacgtccaaa
     6781 ttcttcttgt ccgtcaactg ttagcaagat ttcgtataat gtttcatttc cttggtcatc
     6841 caccaatgtg atgtgttcgt gtccttcgtg ttcgtgggaa tggttatgtt ctgtcataag
     6901 attacctctt ttctttttat tattaaaata attgcttacc tgagccgtct aaataatttt
     6961 gtaaaatcat cacagctgct aatttatcga tgactttttt tcgtttcgct cttgaagtat
     7021 ttgcctgctc tactagcata cgttctgcct gaacagtggt tagtcgttca tcttgataat
     7081 caaccggcaa ggagaatagt tctttgatct tctctccata tgacatggaa gcttctgccc
     7141 ttggtccgat ggaattgttc atattcttag gtaatccaac tacaaaacgg tctacttcgt
     7201 actgcgccac taattccttc aaccgatcaa aaccaaactc ttcttctgcc tcattgattc
     7261 gaatgatttc tactccttga gctgtccagc caaagggatc gctcacagca acgcctactg
     7321 tcttagaacc gacatccaag cccattactc tcataaattc actccatgat ttgagaggta
     7381 gtctttgaca attacttcca tgatttcatc gcgttcgtga cggcgaatca aatttcgtgc
     7441 atcacgataa cgaggaatgt aggccgggtc tccagaaagc aagtagccga caatctgatt
     7501 gattggattg tagccttttt cttccaaagc ccgataaaca atcgctaatg tttcgctcac
     7561 ttcttttttg cgactatcgt caaaaccaaa acgtacggtt tcatctgtaa aacccatgat
     7621 tctacacctc tttcccgcaa ggtaattcca gcttaccttc tacaccctta ttttactcga
     7681 agaaagggaa aactacaaac aaatattcca aacttctgaa taaattaaga aacacgattt
     7741 gtctgatttt tccttttttc tgtggctttt tcaaaagccc tctttccata aagtatacgc
     7801 tatatttctt tgaaagtatg tttttacaca attttttcat aaagttcata aaaaattcga
     7861 attgctgcta ctaatttttc gttttcgttg cttgccctgt ctcataattg atggtgtagc
     7921 catcttgaac attgtagata aagacattga attcaatttt tttgtcttca atgctttctg
     7981 cttccaactg cacacctctt gctaccagtt catttccctc gaaatgagga gtgacacgat
     8041 agcgtacatg atgatttgtt tccttgatat atgccgcaat atcattttcg tgggcaagca
     8101 tgtacggagc attcaacgaa cgtgtccctg tcattaagtt tttaatgtta ttattttgac
     8161 cagtcaactg gaaaccaatc aaatggctgc ggttgtataa ccattgtccg tcacttaatt
     8221 tcttttgttt ccaacctgtc ggatcaatat acaaaggttc tctcttttca gtaggaaaca
     8281 agtcttttcc caacatcgca ttggcttgtc ctactctgtt caatttgtct atatttgaat
     8341 agctttccca tgtccctttg gaaagactaa gatcctcttt tgtaaactcc ggctgattcc
     8401 cattgatttc tatttcttga tgttttccat catattctgg cacatccgct acttgagaag
     8461 cttgcggtgt ggaatcaaaa atggttgtaa tttgttggaa gatatctgaa tctttttgtg
     8521 tggtggaaat accgagtgcc cctgcaatca cagcagcgac gatccccacg atcgccttgg
     8581 ttgttttttt gctcatttct ttactccttt actaacagtt ccctaaatct taacatatgt
     8641 tcccctataa ataaatactg gaaagaaata gaaacagtaa aaaaataaaa ccagttttta
     8701 gacggcttaa gaatacaaaa aaagagacgg attcatcgtc tctactcaat cacaaaacac
     8761 tatttacaag gtatttatta tcattcaaaa aatttttctt aatactcaaa actaaacggc
     8821 ttctgtacga cctcttggta aacagtgttc aaaagcttgc atctgcggta ataagtccaa
     8881 ccaagtcaaa aacataagaa gcttttttgc ttgactattc ttattacttg atgcagggca
     8941 gctttttcac agcctctttt aagcgggtga cgggaatcga acccgcgaca tcagcttggg
     9001 aagctgggat tttaccacta aactacaccc gcgatacaaa aacagtatac gcctctttca
     9061 ctccttcctc aagttttttt attctttacg cttccgatat gttttgtagt aaaataaaac
     9121 taatcaatca attattggag aagatgctat gacgggaaaa gaattagatc tgttgctgga
     9181 cacttgttta ctcgctggga aaatcatgat ggaaagcaat gcggagatgt atcgggtaga
     9241 agatacgatg agtcgaattg ctttagcttc tggaaatttc cgtttggtca gctatgtcac
     9301 tcaaaccggt ctctttattg gtcttgatcg tacctcgacc atccgaatgg aacaaattac
     9361 caaccgttcg attaatttag aaaaagtagt aaaagtaaat gatctttcac gtaaatatgt
     9421 tgcttcagag ctaacattag aagaacttta ctgcgaactg caagcaatcg accgagaccg
     9481 ttctttcttt cctgtctggt tgcaaatcgt tagcgcggca gtgatcagtg gaacaattat
     9541 ggtgttattt ggcggaaaga tgaccgattt acctgtgact ttagtcattg gaggattggg
     9601 atacgcagct tatctatata gtttgaaatt ttttagaatc aaatttttat cggaattttt
     9661 atcttcttta ttgattggca tagccgctat ctacagcgta cgttttggct taggaagcaa
     9721 tcaggatcta atcattatcg gatgtgtgat gcctttagtt cctggagttc aaattacgaa
     9781 cgctatccgt gatttgctag ctggacatta tgtctcagga gtttctcgcg gaacagaagc
     9841 gatgatgact gctacgatga tcggttttgc gattgctttt attttccagc ttttttatta
     9901 ggaaaggaac acaagtatgg ggacgatatt tattcagttt tcttttagtt ttttagcgac
     9961 tgcggcattt gccgttatta caaatgtacc taggcgttca ttgatttgtt gtggtctttc
    10021 tggtgctttt ggctggatga tctactggat ctgtgttcaa ataggcggat ctgctgcttt
    10081 tggttcattg cttggttctt taggagtagc attcatcagc gatcttttct ctaaacgttt
    10141 gaaaatgccc gtaacgattt tcaatattcc cgggatggtt cctctagtcc ctggtggttt
    10201 agcttatcaa gcggttcgaa atctcgttac tgaatcctat cgagaagcgg ctcattatgc
    10261 ggtccaagca attatgattg ctggagcaat cgctttaggt cttgtattat cagaagtctt
    10321 taaccataac atccgtaatt tcaaagtaaa gcgtgacgca atcatgatga aacgaaaaaa
    10381 acggcttttt aaataaacga agtgtaacaa agaaaaggtt gtgaaagaga cgttcagcct
    10441 tgagcgatga caaaatatct cacaaacatt gcgacaagta accgcaggag caataagaag
    10501 taattatgag tattaaaaaa agttttgtaa aaatagcttc tcacattttt acataaaatt
    10561 gacttaatac tgagaaactg acttttgaac accctctatc tgtaaaaaag gggctgcgac
    10621 atgatttttg tcacggcccc tcttctttat tcaaattttt tccaaaacgt tcgttgataa
    10681 taaacgatcc atagagcgga aagtattaga actaaaccga tcacaggaat attcactaac
    10741 caaaatggat aaaacaaagc caacattccg tataagacga taagaataat actgatgatc
    10801 aatgaaccca tcatagttgc gactaaaccc aaacttcctg aacctctgtt aaataattgg
    10861 ttaatattgg tccagttcag cagcagcaac cgataatctc tagcgaaata acgtagtgac
    10921 aataagtagc agccgaataa agaaccaaat agcaagctag ggataaaaaa caaaggcagc
    10981 tgaaaagaca gtccgcttaa taatgcgatc ccaccagtta aaaaactctg caatatccat
    11041 ccgaatctga acttttcttt taaatattgg ttcatagaga ttggcaaaga acggataaac
    11101 agaaaatttt cttgatccaa agaaataaga ttgctgataa aagaagtctg attaactgtt
    11161 aagaaagcca atgcgatccc taccaggaaa acaacaccga ttaacttatt atctaaatca
    11221 gttaatttta tttcgcctcc tagggcaaaa gcaagaataa aaatgattgg agttaacaag
    11281 gaagaagaaa gaacttgcat aatcaaatta ggttctttta ctaattgagc attgtaacta
    11341 aataaaagct gatgcacatt ttggttcttt ttatgggttc tttttatttt gaactcactg
    11401 tttgaatcag tcagttgctc tgtaagcttt ggtactatca gctgttttac agcaaggaaa
    11461 aatcccagag ccgtgatagc tagtattccc caacccaata aaccttgttg agaaagtggc
    11521 tgactggcag catcatagaa tggcaataat atcgaaatcg gatgccgatc tgttaactca
    11581 ttttcaatag tccccgtttg atgattcatc cacatgattc cagcaaatgc gatcagcatc
    11641 gagctgccaa gcaagatact agaaactgtt tttctatgtt ttttaaacac tgctgttcga
    11701 gccaaaccaa aaacaatcaa actagccaga caaaaaagaa taatcaaaaa tagagaaaac
    11761 aaaaggattg agataatgat agtaagcaaa atccctagat cagctcgcca tccggttaaa
    11821 ataaacatga ctagaagcgg aaaaacaaaa ggaatgatgg tcaaagcaac taccaaaatc
    11881 tttgctgaaa aaacctgttc ttgtccaaaa ggcagaggca aaaatacttg caggtcattt
    11941 ccttcaaaaa agatattata aatgactgat atactttgag ttaaacctaa cataccaaaa
    12001 agtgccacgt aataagtaaa gaaacctggc aatttaacaa agttcatagc aaacatagca
    12061 aatccgtaaa tcagtaaaaa aatcagccct gaaaaaagat tttgagacaa aagatagata
    12121 gttaatcgct ttcccttttt tcctttctct cttgctttat tcgtgagctg tggattaaca
    12181 tataaaagat taactcgaat taattctaat aattgtcgct tatacatacg gtccatctcc
    12241 tgtcgcttgc cttccagcca ttttcaaata aatcttttct agcgtttcat caggtgagcc
    12301 attcaataat tcactaactg ccccctgata aatcaattca cctttcttca aaatagccag
    12361 tttatcgcat aactgttgtg cggtatccaa tacatgtgta gaaaaaatga ctgtttttcc
    12421 ttttgcggca tgagccttca tcatttcttt caaatcaaat gctgcttgag gatcaagtcc
    12481 ttgcaaaggt tcatccaata cccaaatatc tggatctggc agcaacgtgc cgatcaaaat
    12541 cgttttttga cgcattccat gtgaaaaact tgcgattgtt tcattttgat gactatacat
    12601 atcaaaaagc gctgtcaatt ctgctaaacg cttttctttt ttttgtgtgt ctaattcata
    12661 ggcagctgaa atcaagtccc aatattctcc agccgtcaat tgtaaaaaaa tatctgggga
    12721 atcaggcaca taaccgattt tttgtttgat actcaatcga ttttccgtca atttcattcc
    12781 atctactaaa atcgttcccg atgatggttc aataatacta actaaacttt taatcgtagt
    12841 tgattttccg gcaccattat gtcctaaaaa tccaaaaatt tccccttgtc ttatcgagag
    12901 agacaagtct tttaatgctt gttttttccc gtaactttta cttacattga tgaattcaat
    12961 catttctgta ctccttttgt ttgttattac tttttattgt acccttcgtt attggtggag
    13021 taaatgtcaa aaagagtgca taacaaaaaa ggatagaaac gactaggctg ctacttgggt
    13081 tgttctcttc tacaggaaaa cactcttcta acaggtaaga tatgagtctt tgttttcttt
    13141 aaaatgattc attttctatc ttttcttgca taaaattctt tagtttatca atacttctct
    13201 ttcccatctc tagttgttct ccatttgcaa atgttactat ttcgtttact ctgctaatag
    13261 atgtaatgtt ttcagtatta atgatataag cttttaaatc tttacataaa tactcatatt
    13321 cgagtgattt tttatatttt ttaattgtgc cgtttacgag aatctcttca tctttcaata
    13381 ctaggcctat tttattttta taacctggca tgctcattag ataaaggatt tcatcgtaat
    13441 ggatatagtg atcgctatta ccataacgga cagttgtcca cctttttttc gtagcatgtt
    13501 gaacaggata gttttctttt tctttcaata agtaactttg gatgcttttt ttattttttt
    13561 cataagtgaa ttcttttatc aaataaccac taggcgtaat ctttcgatta ataacagtta
    13621 ttgctttatt cggatgagac gttagataaa caattttaca cttttcattc ttttctctaa
    13681 gcatttcgcc tagttcgata ccgctataat aggcgttcaa atcgatgtct aggaaaaaaa
    13741 tatctgagga atagatttca ttatttttaa tgaattcaac aaattgtata acgttcaatc
    13801 cctgaacgat ctcaaatttc caaggactta ataaaattct ggattcttcc aaaatttcta
    13861 ctagcatttt ttcaaaaacc aaactatcat ccaaaataaa tatacgcgtt accataaaaa
    13921 gtacctccac tcgatatttt actatcaaaa tcatttcata caacttttca acatacatgt
    13981 taggaataaa ttgtaattct cttcacaatc ttttccaaca cctttgaaac cgatatcaaa
    14041 tactttcagg atgctgcttc taaaataaat atcaaacaaa tgaatacatc atgcaaaaaa
    14101 agccagacaa ccgacgtcct tgcgtgtcag ctgtctgagc tatgtttatt tattttgttc
    14161 ttttaaaaag aacgatacac atactgattt ttcggagcag agactttctc agcgtttgta
    14221 acaactacag atgggatttc acgcttgaat ggttgataat agtctgattg gatggttcct
    14281 tctattttat accattcatc atttttaacc gttactcctt ccggcatatg cgtcaacaaa
    14341 ccaaagactc ctgaatctgc cacacaatgg ataatgccga atcggaacaa gaagatatct
    14401 gtattgctat cgttcggtgt ttggtaaaca aatccttcat agctgatttt tttgccaatg
    14461 aattcgctag ggtaattata aattagctcc ataacttcta aatagttttc atctgtgatc
    14521 ttgatagttt gcttatcccc atacttttct ttcaactcat tcatttggtc taagtagtct
    14581 gatttgttga aataaatact ggtatccggc tttaagtatt gtgtctgcat ctccggatcc
    14641 cctactgatt ctttacttac tgggaaatta aatcctttgg cttctacgat cgtagtgtcg
    14701 agactaactg ttggaaacaa cgtccccacg attactggca aagctagcag tacataagcg
    14761 attccacgtt gataaggttt tgacaaccca tggtcatgat cgtgctcgtg tgccttttct
    14821 tctgcttttc cgtttttgac ccacaaatac agctgaacta aagccaaaag agcagataaa
    14881 atcattgaaa gaatagctaa gtagcgataa tggacattga tatattgatc gagtttgcca
    14941 gaaacttgca agtacatcat caattcgaaa tacccagata aaatcaaaaa ccgcagcata
    15001 ttttccctcc taaataaata atgtgaatcc aataacgcta agggcaatca gtccgatcag
    15061 cgataaaata aagcgtcctg taaaatatcg tttcatcatc agtagatttt tgatatcgac
    15121 cattgggcca aagaccaaaa aggcagctac aggtgctgtg ccgaacagac ttaacaaaga
    15181 agatcctata aacgcatctg cttctgagca aagagacatc gtaaaggcca ataacagcat
    15241 gatgataatg gctaaaatct ttgttgagcc gagcgttagc ataattctag ttggtaaata
    15301 agtctgcatc gcagctgcga ttaatccgcc aatgatcaaa taacgacctg tatcaaaaaa
    15361 ttcgtcgatt gaatgtgtca acacatgccc agtttgatgg atcagctgat tttttggttc
    15421 attcttctca ctttgttctt cttcatggac atgatgatgg tgattttctg ctactttaga
    15481 cgtgattttt tctttcaata cagcgtcttt ttgaaaatag gcaagccaga caccaatcac
    15541 aagggcaaca aaaaagctgc ctatcacccg ccagcttgcc atctgccaag aattgccaaa
    15601 tgcgataaaa gtggaaaaaa tcacgattgg attgatgatc ggagcagtca gcataaaagc
    15661 aaacgctgtg tgaactggta cgcctttttt aacaaattga tgaacgatcg gtacgatgcc
    15721 gcattcacat gaaggaaaga aaaatcctaa gatcgaacca gtaaaaatag agaagatacg
    15781 attttcagga agccatctct tcacacgttc tggggtcaaa aatacttgta aggcgcctga
    15841 gataagacag cctaataata cgaaaggtaa tgcttcaata acgatcgata agaaaatcgt
    15901 actcatctgc aaaaccgaat ttggaataaa agaaaacatg agtttcctcc tttttatttc
    15961 tagttcattc gctttgcaaa tatacgcttt tattcaaaaa aaacaagttg ctctgtaaat
    16021 ttttacacaa ctcttatttt ttaccaaagc acttcaattg actttttctg ttattcccat
    16081 taaattaagg tctcatacat atcacttcta tcataaaagg gagaataaaa atgaaacaaa
    16141 ccactaactt gactgaagtt caagacatac tgcaacagtc atccgttgcc gtcatttatc
    16201 tttcgatgcc taattgcagt gtttgtcacg ctgttcgtcc acgactagag gaacttctca
    16261 cacattatga tattccggca cttcatttgg atgcatttga aacaccagaa gtagctagta
    16321 aatttgaagt gctgactgcg ccagttgtcc tgatttttca tcaaggaaaa gaagtctttc
    16381 ggcaagcacg gttcatcgac tttaaaaaaa tccgttatct actagatgaa ctgacagcag
    16441 aagatgattc cctttcttat gaagaaatct tccgaacaaa ataatcgttg tccaaacaga
    16501 aaaaagcgat tgaacaagtg aaagttttgc cttcatttgt tcaatcgctt tttattttta
    16561 ttctgctgta ttttcggaat gcgcatttgc gtcagaatgc aagaaataga tcaaggtttg
    16621 caattcattc gtcaaatcaa cattttggat caatacatct gacggagcat tcaatcgagc
    16681 agcggtaaag ttcaagattc ctttgattcc tgcttccgtt aactgattga ctacttcttg
    16741 tgctctttca gcgggtatcg ttaaaattgc tacttcgatt tgttggatct tgatttgttc
    16801 tgacatatct tccattggat aaacggggat accgtctacg atgcgcccaa cgatttcctc
    16861 attcacatca aaagcacaac tgatacgtat gctattactt tgatggaatt tgtattttag
    16921 taaagcactt cccaagttcc ctacaccgat cagcgcaaca tttgtcaatt gatcgtcgtt
    16981 taatgtcttt gcaaaaaagt gcataagatc ttctacatca taaccatacc cacgttttcc
    17041 taattcgcca aaataagaaa aatctcgacg gatcgttgca ctatctactt gaacggcttc
    17101 acttaattca gttgaagaca cttttttctt tccagcatta tttaaaatcc ttaaatagcg
    17161 ataatacaaa ggcagtcttt tagctgtcgc ctttggaata atatgatctt tcacgaaatg
    17221 acctccacgt attgtctaat tacatctttg atttctccat catagcagta tttataggaa
    17281 aaaatcaatt ccattgctca gatattgtga aattttattc tttattcatt tatttatcat
    17341 ttctgcaaat ctcgcagtat aatggaattt atggtagact actaattgaa aaatagtttt
    17401 ttatttgagg agaattcaca aagtagaaaa gaggattcca tgatattatt acaagccaat
    17461 cagattgctc gccattttgg tgcagaagtc ttatttgaaa acatacattt agaaatcagt
    17521 acaggcgctc gtgtcgcttt agttggcaga aatggcgctg gaaaatcgac tttattaaag
    17581 atcattgctg gaattgaagc acctgatgaa ggaaagatcg ctaaaaataa aacagcgact
    17641 ctcggatacc tcgctcagga cacaggactt gattccaatg aaacagtttg gaacgagatg
    17701 ctgaaagctt ttgaacaagt tagacaaatg gaagagcgga tgcgcgagct agagattttc
    17761 attagcgaag gtacgccaga cacacctgct tatagttctt atttaaaaga atacgaccgg
    17821 ctgcaacacg atttttcaga tcgaaacggc tatggttatg aaaatgaaat tcgttccgta
    17881 ttgcatgggt ttaaattcga ttcttcattt tatgatcaga aaatcgatac tttatcaggc
    17941 ggacagcgca cacgtttagc tttagctcgc atgcttttgc aacaccctga cattttgatt
    18001 ctagacgaac caacgaacca tttagatatc gagacacttt cctggcttga gaattatctg
    18061 caaagctata ctggtgcatt attgattgtt tcccatgacc gttatttttt agataaagta
    18121 gtcaatgaag tctatgaatt aagcagaaga aaaatgcgcc attacaaagg taactattcg
    18181 aaatatctag atttgaaagc agaacagtta gcaagtgaat ggaaggctta tgaaaagcag
    18241 caaacagaaa tccataaatt agaagacttc gttgctcgga accttgttcg cgcctctacc
    18301 acaaaacgag cgcaaagccg cagaaagaca ttagaaaaaa tggaccggct ggatcgtcct
    18361 caaggggatg aaaaacaagc aaactttcta ttcagtatag aaaagccttc tggaaatgta
    18421 gtcttgcaaa tcgaagatgc cgctatcgga tatgataatg tcaccttatc tgaaccaatc
    18481 aatctagata tccgtcgtca agaagcgatt gctctagtag gtccgaatgg tatcgggaaa
    18541 tcgacgttgc tgaaatcaat cattcaccag atccctttta tccgtggaaa agaaacgcta
    18601 gggacaaatg tcaccatcgg ctattacgat caaggtcaag ctgatctgca tacaaacaag
    18661 acgatcctag cagaattgtg ggatgagcat cctaccactc cagaaaaaga gatccgaagt
    18721 gctttaggtg ctttcttatt ttctggcgaa gatgtagaaa aaccaatccc tctgctaagt
    18781 ggcggtgaaa aagcacgggt tgctttagcc aagttagcga tgaacaaaga aaattttttg
    18841 attttggatg aaccaacaaa ccatttagat attgacaaca aagaagtatt agaaaatgca
    18901 ttgatcgatt atgacggcac actgcttttt gtttcccatg accgatattt catcaatcgg
    18961 atcgcgacaa aagtcgtcga actctctgaa aatggcagca aagtctatct aggagactat
    19021 gattattatt tagagaaaaa gcaggaagaa gaagaaatcg ccgaattgtt ggcacaagat
    19081 gagccagaat ctgctcctgc tcccacgaac aaatctgatt attaccaaag caaagaacag
    19141 caaaagctta tccgtagcct tcaacgaaaa attactcagg ttgaagaaga aatggcgcgg
    19201 atcgatgaat ggatcgatca gttggaacaa gaaatggtcg cgccagaaaa cctggaagat
    19261 cacgtaaaac tcaatgagct gaaccaagat ttagaagctg ctagacaaga tcaagaaaca
    19321 aaattgacgg aatgggaaga actaagcttg gaattagaag aatatgaaaa ttaacgaaaa
    19381 aaccaaaaca gaaaccattc atgctgtttt ggtttttctt tttttatttt atcaaacaaa
    19441 aattccatat ttaccttttt caccaataag aagaccaatt actagtcatt ctcactaaag
    19501 cttctaaata atagtaatca ccccaaatac aaggtgtatt taccccatta tttttgggct
    19561 tattataaac agactgtgtt aaaaagccat cttcttcttc cgttgtagca tatctttctt
    19621 tcaattgtcg cataattttc caaacagcag actggtataa gttcttttct gttttgtcta
    19681 atagacctga ttctattaat tctaataatc cacaaaccat gatcaccata ctagatgtat
    19741 ctttttcttc tctgctagaa tcatcaaaga tcaaatccca ataacaaatg gtatcttctg
    19801 gcaaccgatt cataaaataa ttggctaata ttttgcttaa atcaaaatat ttttgttctt
    19861 tcaaataacg ataacttaat gcaaaaccgt aaatccccca tgcttggcct ctagcccaac
    19921 aggaattatc actataacct tgatgcgtac ttccatatct tggttttcca tctttgacat
    19981 ccatataaaa tgtatgataa gttgaaccat cttctcttac taaataatct gctgcatttt
    20041 gaatatgagc ctgagcagct tttttatatt tttgatttcc agtttcttca ctcgcccaat
    20101 acaataatgg aagattcatg caacaatcca tgatcattct accttgttgt gaaggatcac
    20161 taggatctcc ccaagcttgt ataactcctg ccttctccca aaaacgctga agtaatgttt
    20221 ctgcagcatc aattgaagct tccttcattt ttcgatttcc cgttatttta taatctgcta
    20281 cggttgataa agaatacaaa aaaccaagat cgtgatgatt agtgataatt tgacagtcaa
    20341 tcctgtggat aaaatctata tctgcttttt cagctgtttg ttgaaataaa ggatcacccg
    20401 tcttctcaaa taacaaccaa ttaattcctg tccaaaatcc ttctgtccaa ccaatattgt
    20461 ctcttaatgg ataacgtcct tttataggac actcgcttgg gaattgatac tgactgttac
    20521 tgatacgttt gattaacctc tccgtgtgtc tgcgacaacg ctcgatctca ttattccatt
    20581 ctttttccat taattcttta taacattcat ttatccattt ttctggatgt attatctctt
    20641 caaacatcac tttatcaagc atttccccac cagcctttag cataatttta gtcttttttt
    20701 attgttttta ttttggatag ttagcccggt tgtcccatca aaatcaattt tgggacgatt
    20761 gttccatact tctaaactat aatcccgatc ttgatgagca accactcctg ttccgagcca
    20821 atgggttcct tttccaaatg taccctctaa tccaggaata aaagtgtttt cccatgtcat
    20881 caagtttgta ttgggcaaac ccataatagc tgtggatttt cgtttaatag tatcgtcact
    20941 taaacagata atacctgaaa agccagcttc actcacccta tagttttcct tgtcttcttc
    21001 tatgattatt ccttctttct caggagaaca ttcaatagca aaccctgtct catatagaac
    21061 atattctgta tctaattcta ctttgtgtac gcgaatctgc cagccttcaa aaggaataag
    21121 ccaagttctg atttttgcct gtaaagtagg ttcccatgta gaggcaagta cctctttctt
    21181 acatacaatg tctcgtacat tatttcgaac gatgaactgt ccataggttt tgatgctctc
    21241 attcttaaaa ctggtcgtga tatatgtgtc cgctcgctga atccctagca ttgaatccat
    21301 tgccatttct gctttcaatt gattcgctcg cggtatcgaa aaaccaaatt gactagaata
    21361 agcaaatttt aaatattttt cattggtcaa tgtatggaaa tttggcccta attgtcccgc
    21421 attcaaaaaa aatgtatgtc cattatcatg ttgtatgagc aaattggctt ctgaaagaag
    21481 tttagttcct tcacgaatag gcataggcat ttctttagcc gtccaatact catggtcttc
    21541 aggtagcgcc aacaataaaa aaatcttatt cagccataat ggagataacg tactattata
    21601 gggttcagtc aatgtcaatt gagggtaggt ataaccaata ttcaggatac ctttttcatc
    21661 aaatatttct ttttgcatcc accatcttat attccgattg actaacccct tgatttcacc
    21721 taatttaaaa ggcaaaagat cggcccacaa catagctgac caaaagcttg aaactgcaaa
    21781 acgataaatc atgcttcgac caaaaggcac atttgcacca tcttccgcaa aaaaatgaat
    21841 atagtctcta gaaaaatcaa tagctcggtt cttatatatc tggctaatgg gcttttctgg
    21901 ttctagtacg ctataaatca aaccataata atgaaatgca aaaggattat aataatcttg
    21961 gcgacctctt gaactgtctc gataccagcc attttgaaga taacaatttt ctatattatc
    22021 aaatgcttct ttcagtgctg attctttaac tggataacca aatttttgga taattgttcc
    22081 gacaattatc ttaaaaaatt gccaattacc atctgcacat tcatattcaa aaatttgcga
    22141 gaaccaatga ataatctggc ttttttctcc tgtatctaat tttttccata ataattgttc
    22201 atgatgatat aacgctaata acattgcggg catttctacc atacgctggt ctctttcatg
    22261 tatttctccc caataatcgg gatgggaagg atctgttcca gcttttattc ctgatagaat
    22321 taatttcaac catcgttcat ctaattggtc aattgcgggc cccgctcccc ataaaacccg
    22381 agagaatcct tccattcctg caattttatt tccatatcct accccttgat gtatataatc
    22441 aatttgtgcg ccacttgctc gataaaacgg ctggagtggt tgatttatct ttttaaataa
    22501 gtcaaagtaa tcctgtctgg tccttaatag tggtactgga tgtttcattg tattttctcc
    22561 tctttttact accaaatagc taattccgac tgttctaatc gaagtaagcc ttcaataaaa
    22621 aaataatcag aatagatcag tggcacatta taagtcagtt ctctatggta tgcttctgct
    22681 ccgcctccaa tgatcccatc ttcatccata ttccaatttg catagtgctt ttctgtcttt
    22741 tggagtatat tgatagcagc ttcatgatac atttttgatc gtacagagga aacatatcct
    22801 gctaattcca gaagaccgca tgcagcgcat actccagcac tggtatcaat attcgaatct
    22861 tttacagcaa aatcaatgag aggaatataa ccagtttttt cgattttctg aataaaattt
    22921 actgctatct tttctgctat agctagatag tacggatttt ttgtgtaacg ataacttaac
    22981 gcgaatccat ataaagccca tgcatttcct ctgctccaac aagaattctc attgtatcct
    23041 tgcccgccaa gtaaccgaat atatttccct gattctgcat caaattctgc gatatgcccg
    23101 gttgatccat ctttcctcac tagatactta gctgttgtat ctgcatgctg ccgagcaatt
    23161 ttagagaacc tcggatcacc agtttccctc gaagcccaat acagtaatgg taaattcatc
    23221 atactatcaa taatcatcca accaggttta tggtcattcc atgctactaa atagttccct
    23281 gaaatattat agcgtccagc aagtaccgta gctgcatgta accctagttg tcgactatgt
    23341 ttgttttgag actgttgata atgtgcaata gccgatggta agaataaaaa acctacatca
    23401 tgatgaacgt ctgaaaaatg gtacagggca gattctaata attgttcttg ttgtattgct
    23461 gcctctttga acttttctcg ttttgtcttg tgaaagagct gccataaaat accactccaa
    23521 aaaccattgg tccaccagtc gattcctctg tcctttatta cgtctgtata ttttccgttg
    23581 taagaaatat atggaatttt attccccatt ctgtctaatt ccgcatctat cttttcagaa
    23641 gctacattca aaatttttct tgaccattcc ttcgtctgtt cattcatgga tatcccgcca
    23701 ttcttttaat ttttatgaat gtggagttgc tcctagaacg aactagatgg ttaacattcg
    23761 ttattttttt tacttatttg cagctccaat gtcattctcc ttaaatttag actgcgccca
    23821 cacataaatc aagaccattg ggatcactga agttgctaaa attagcataa ttgcgccata
    23881 attaattggc tcacctgtac tagaaaccgt catatttttg ataattaagg gaaccgtata
    23941 tttatcttta tctgaaagca tgactaatgg aaatagatat tgattccaac ttcccataaa
    24001 gataagtaaa ccactagtaa taatcgtagg tgataaaatc ggtacagcga ttttcgtaaa
    24061 aatcacccac tcattggcac catcgattct ggcagcctcc atcagtgctg taggaaattg
    24121 actgctatat tgtcgcatca taaacactga agttgtactg gcaatcgaag ggatgatcac
    24181 acctgcataa gtattcgata atcctaaacg actcataatg taaaataaag gaatcaatgt
    24241 ggtaaaaaaa ggcaccatcc tagatagcat aactgtattg aacaacagtt tattccccat
    24301 aaaacgatac tttgctaatg cataacccgc cattgtagaa ctggctgtag ctgaaactgt
    24361 actgattaca gcaataacaa tcgaattcca aataatcaag ggataattat atctcgccat
    24421 gatggcttgc cagtttttta atagcatatc cccaaacttg aaattgattc cgccacttaa
    24481 aatttcttga ttactttgcg ttgaagctac aatcatatat aaatatggca tcaaaaatat
    24541 taaggcacca aacacaataa tgacgtacgt aaaaattctg ctttgtatct tttgtttagt
    24601 catttttatc acctccgaat ttaaattgaa tagccgataa tataacagat acaaacacca
    24661 tcgtccaggc aatagcagag gcttttccaa aatcaaccat ttcaaaactt gttttatata
    24721 aattgactcc tacgaccaaa gctgaatttt ctggtccatt ggaagtccca aagaatatat
    24781 ttgggatatt gaaattaccc aagccattga tagtggctaa aactgtggcc attaaaatga
    24841 ttggctttag catggggatc gttaataaaa agaattgccg aaatttcgat gctccatcta
    24901 tttcagcact ttcgtatatc tctttagaaa tcccttgaag accagctaaa aataaaatag
    24961 tgttatatcc tacactaagc caaatcatcg tgatgatcat tgatattcgt atccaaaaag
    25021 gatttgttaa ccactgtaca ccgttaattc ctagcgcatt caaaaatcca ttgaataacc
    25081 cagtcggatt gaaaaaagtc atgaatacgg aagctacagc caccgttgat gtaatggttg
    25141 gaatataata gacagttcta aaaaatcctt ttccttttaa ctgtgctgaa ttcaataaaa
    25201 cagcaaagta aatagctaat agcatcgtta ttggaattga tcctgccatg ataatcaaaa
    25261 cattcgtgta agatttaata aacaatggat ctcggaagat agctagataa ttgtcaaatc
    25321 caacaaattc aaatgagtag ccaattttca taaaactaaa agcaaacgag ataatgatgg
    25381 gaatcaaaat cgtcacaata aagaaaataa caaaaggtgt gaccaatcca taagcacttt
    25441 ttttatgaga cgtatctgtt gcttttttct tgctgttctt tattgtcttg cttggtaacg
    25501 aagattgcat catcgttaag tcctcctaat attttttaat caccagttaa ttttacgccg
    25561 tacttatgag caaaattttc tgcttctttt agatatgaat tttcaaaatc acctgtttgc
    25621 caatagttat atgcagcggt agtcaaataa gacgtagcat ctgaataagt ggctggtaaa
    25681 acagtatttg atgtattttc attgatttta ttcaatagtt catagtattt ttgtcctcct
    25741 tagtagtcaa actttttctc agctgcttct gtttgatagg ccaatgagtt agatgtagcc
    25801 acgcccagct ccagtgcgtt ggctaagcat tcttcatctg tcagcataaa tgtaagaaat
    25861 tgttgtgcca cttgtggatt tttggcatct ttagggacat aaaaacttga tcctcctgat
    25921 actgcacctt ttcctggatt ttcttttgaa aaaggaatca gtggagccat gcgccatttg
    25981 tcagatgcat caggaaaatt caaagacata ttcgtagcat accagccgcc agcaacaaac
    26041 attgcagatt cttgaaatgc tttctcagca tcttgtgcac cgtagaaatg tacaatatct
    26101 gagtcgatca tttctttgat taactttgca gcatgtttcg cttctttagt attcagacgg
    26161 atatttcctt cttcatcaag caaagattga ttctgctggg ctagcatgtt gataaacaat
    26221 tcttggtccc cagattctgg caaggcaatt ccaaaactac cagttttttc ttttaatgct
    26281 tttgccacct ggatatattc ttcccaagaa ctgatttctt caaaatctac acctatttca
    26341 gcaaattttt ttggttgata ataacaaagt gctggggtaa aatcactagc ccaaggaata
    26401 atcgtttcat tactcgtttg ttgacgcaaa atattcaatt tttgatcata aaactgatcg
    26461 ccatatttgt cataaaatcc atattcatca gcactataaa aagcatcttg aaatttttcc
    26521 agcacacctg ctgctgaagt gtctgccatg gagataatat ccggcatatc ttcgtgtgtt
    26581 accaattttg gggtgatcat ttgtactgtt gctgttcccg aagctacatt cacaattttt
    26641 aagtcaatat ttgctccagt tttgcggtta tagatttttg cagcattttc aacatagttt
    26701 gtcagtttgc cgtttgcgac atatacttct aatgtagtgg ctttttttat cattggatct
    26761 gcgatgtttg ctgttacttc ttcgttagtt tttccacatg cacttaagct gaataatagg
    26821 gtaatgttta gcaaagctat caccaatttg tatctttttt tcatgtctat cccttctttc
    26881 ttttcaagtt cttatataaa acgctttcac ttatgtatat taacggaatt agtaacacaa
    26941 atatttatat aaaaaaatat tttacttgtc gttttggctt ttcatttttg ttataatagt
    27001 cattaattat aaatagaaag ctttttatat aaaaagagaa aggaaagtgg ttcttttgtt
    27061 aacaaacaac caatcctcca atcttgtcaa aaaaacacta acctattcca tagataccca
    27121 tcttcattta tctgctgctc cctattttcg actactaata aatgaaagcg ctaatttata
    27181 tgctgaattt gcacataaat ctatagaact agataatcct tcccttttat tcattgataa
    27241 acagcaagaa gtccaaatct tttctactca aaaggaaaca caaactttta tcattatcca
    27301 gttttatttt gatgagcaat tattagcaca tattacggga caatcttttt ctatagatca
    27361 agaaataaca tctatcatta cttttctgaa aaatgaacag ttcaaaattg attatctaaa
    27421 acatagtaag ccagctattt cctatgatca ggaaatcaat tcttttttca tactatgcca
    27481 acagatgagc attcatttgc tttccttatt agttacttat tgtctgcaaa aaattcaatt
    27541 gtcttcctta ccaaggcatt atcaacagga acttgcttta ttgacaatac cttcaaatga
    27601 gaaagtcatt cctaaaaaaa agcatattct ttattcagat gaattagttg atccaatcat
    27661 acgatattta gaagaacatc tttcagaaaa ttttttgctt gaacaattag cccaaatttt
    27721 ttttatcagt ccagccagct taaaaaaaca ttttagaaaa gtgaatggaa aaagtgttat
    27781 tagctatttt aaagaactaa aaatggaaaa agcaaaacgt ctcgtgctag aaaatgaatt
    27841 aacctataca gaaattgcag atatattagg atttcattcg atccatcatt tttcttcagc
    27901 ttttaaaaaa tatacagggt tatccccttc aaagtattat ctatccttaa aatagaaaaa
    27961 aatttttagg ttttatccac aaattatttc tctatatttt ctgtctattc aaaataaaca
    28021 ataattttcc ggcacaatta tttgctgttt tttaccataa catagtacga tcaacgtaga
    28081 aaaggagatg tttttatgaa agttgttgtc attggcggtg tagctggcgg tccttcattt
    28141 gccactcgtt tccgtcgatt gaatgaagcg cacgaaatca ttatctatga acgcggagaa
    28201 aatatttctt atgcaagttg tgctttgcct tattatttag gaggtgtgat cactgaccgt
    28261 gactcgctga tcgaacgtac accagaaata ttgaaaacaa aaaacaacat cgatgtcttt
    28321 actaaacacg aagtaacagc aatcgatcct tctactaagc gattaacagt taaagaccta
    28381 tccacaaatg aagaaataaa aacagattac gataagttga tcatctcttc tggtgctaga
    28441 ccagattatc cggatattcc cggagttttt gaagcagaaa acggctttgt cctccgtagt
    28501 gtgacggatg cggatcgaat caaatcgttc cttgaagaaa aaaatccaca acatgtcgtc
    28561 attcttggtg caggtgttat gggtctggaa ttagctgaga atttcaagca tcgcggctta
    28621 gacgtgactt taatcgatca attgccacaa gtcgctttcc cttatgatcc agaaattgct
    28681 aatttagttt atgacaaatt gctgaaagaa ggattagccg ttcatttaga aacaagagtt
    28741 actgagatcc gtgataaagg tcgagaaata gtattatcag atggctccgt cctttctgct
    28801 gatatgctaa tttttgctgt tggtgtttct ccgaagaatg aagtggtgaa agcaaccggc
    28861 atacaattat cagatacagg tcagatcatt gttgatgacc agttacaaac caatctgccg
    28921 gacatctatg cgattggcga tattatcgaa acgactagtg tagtgactgg tcagccgatc
    28981 caaagtatgc tttccagtgc ggccaatcgt caaggacata tgctggcaga tattttaaat
    29041 ggtacgccta tgcgctatcg cggatatatt ggtgcaggtg tcgcaaaaat ctttgatcat
    29101 acagcaagtt atgcgggaat gacagaacat gaattaaaag catcaggcat aacaaattat
    29161 aaaactgttt ttatcactcc ctttgaccat gcctatttct atccagaagc tacaagatta
    29221 aatctaaagc tgatttttga tgcagatagc ggtcgtattt taggtggaca agcatttgga
    29281 gaaaaaggtg tcgataaacg gatgggagaa ctttctgtag caatcaccgg aaacttgaca
    29341 gtctttgatt tgcctgattt ggagttgcct tactctccac cgtattctac tacccgtgac
    29401 ccgttgaata tagctggtta tgtcgcaatc aatcaaatga cgaatatcgt agaaacgatc
    29461 aaagcaagtg atatacccga aaacgatttg aaagaagcgt tctttttaga tatacgtgaa
    29521 cctaataaag caccttctgg cagtatctcg gcaacaaaaa atattccaat gaatgagtta
    29581 cgagatcgaa tcaatgaaat cccaaaagat aaaaaagttt acattacttt cagaagagga
    29641 ttgaatactt atacttctgc ccgaatcttg gcaggtttgg gtatcaaagc gattttgatt
    29701 gaagaataaa aaaacgaccg cggtgggtag ccgcggtcta ggagttattt ttacttgagg
    29761 agtaaaaatg aaaaagtata tagggttgtt ggttggtatg tatatagaat agaagataaa
    29821 tgtgaagaaa ttgtgcatta tttcttcaca tttattaggt tatagtttaa gaaagtttaa
    29881 acattgcgca atttaattat tttagctgtt tcttttcata aaaaaacatg ttgtgactct
    29941 gagacaaatc atagtccata gtgatgtctc tagagtaact acgcctaata ttacgaggca
    30001 tcatattaat ctatcagcac tagtacctaa actagcatcc ctttcgaata ttttttctta
    30061 tataataaag tttgctttca ctcctctaat ctcaccaaat cgactggctt gcgttgtgtg
    30121 atactaggaa ctgattggtt tagaacgact tttctattgt aatcatattt ttcgccatcc
    30181 tcgttgtctt ctaatccttc aatgaatgat ccctaatgtg tgtattaaat aatttaattt
    30241 ttttgttcct atttattta
//
LOCUS       DACDYI010000036        30113 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.036, whole genome shotgun sequence.
ACCESSION   DACDYI010000036 DACDYI010000000
VERSION     DACDYI010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.036"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(203..1654)
                     /gene="cls"
                     /locus_tag="IWM52_001496"
     CDS             complement(203..1654)
                     /gene="cls"
                     /locus_tag="IWM52_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP9973752.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            1995..4661
                     /locus_tag="IWM52_001497"
     CDS             1995..4661
                     /locus_tag="IWM52_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9973753.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5025..5777
                     /locus_tag="IWM52_001498"
     CDS             5025..5777
                     /locus_tag="IWM52_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9973754.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGGFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(5799..6953)
                     /locus_tag="IWM52_001499"
     CDS             complement(5799..6953)
                     /locus_tag="IWM52_001499"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAP9973755.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            7138..9579
                     /locus_tag="IWM52_001500"
     CDS             7138..9579
                     /locus_tag="IWM52_001500"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAP9973756.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(9909..10613)
                     /locus_tag="IWM52_001501"
     CDS             complement(9909..10613)
                     /locus_tag="IWM52_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973757.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYSLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(10800..11045)
                     /locus_tag="IWM52_001502"
     CDS             complement(10800..11045)
                     /locus_tag="IWM52_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973758.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(11062..11913)
                     /locus_tag="IWM52_001503"
     CDS             complement(11062..11913)
                     /locus_tag="IWM52_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP9973759.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            12079..13083
                     /locus_tag="IWM52_001504"
     CDS             12079..13083
                     /locus_tag="IWM52_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAP9973760.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(13324..13680)
                     /locus_tag="IWM52_001505"
     CDS             complement(13324..13680)
                     /locus_tag="IWM52_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9973761.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            14048..15235
                     /gene="tuf"
                     /locus_tag="IWM52_001506"
     CDS             14048..15235
                     /gene="tuf"
                     /locus_tag="IWM52_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP9973762.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(15423..18014)
                     /gene="mprF"
                     /locus_tag="IWM52_001507"
     CDS             complement(15423..18014)
                     /gene="mprF"
                     /locus_tag="IWM52_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP9973763.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(18040..18759)
                     /locus_tag="IWM52_001508"
     CDS             complement(18040..18759)
                     /locus_tag="IWM52_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9973764.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            19079..19600
                     /locus_tag="IWM52_001509"
     CDS             19079..19600
                     /locus_tag="IWM52_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAP9973765.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            19659..20519
                     /locus_tag="IWM52_001510"
     CDS             19659..20519
                     /locus_tag="IWM52_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAP9973766.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            20626..23019
                     /locus_tag="IWM52_001511"
     CDS             20626..23019
                     /locus_tag="IWM52_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9973767.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            23201..25042
                     /locus_tag="IWM52_001512"
     CDS             23201..25042
                     /locus_tag="IWM52_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP9973768.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            25066..26175
                     /gene="rpoD"
                     /locus_tag="IWM52_001513"
     CDS             25066..26175
                     /gene="rpoD"
                     /locus_tag="IWM52_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAP9973769.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            26395..28494
                     /locus_tag="IWM52_001514"
     CDS             26395..28494
                     /locus_tag="IWM52_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP9973770.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            28690..29553
                     /locus_tag="IWM52_001515"
     CDS             28690..29553
                     /locus_tag="IWM52_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9973771.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            29658..>30113
                     /locus_tag="IWM52_001516"
     CDS             29658..>30113
                     /locus_tag="IWM52_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP9973772.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEK"
BASE COUNT        10009 a         5314 c         6264 g         8526 t
ORIGIN      
        1 gaaagaggtc gtgagcctgc cgtccagctc cgagtcacaa ggaacaagtt caaaaaacag
       61 cttctcatgt ttgttgaata ttgtgacttg tgaccgagga gttggcagga gaacaccgtt
      121 tgttcggaag aagggactgt gacaagattt ttgtcacagt ccctttaatc ttagtatatt
      181 tttctctact tgaataaaaa aattatagga ttggagaaaa taatcgagaa atttgttgct
      241 tgaataatag ccagttagac atttccttga ctgtttcagt tgtcatggat gaacaatgtg
      301 tctcatcttc tttgaactga gtagcaagtt cttctaaaaa ctcaggatcg taaatgacag
      361 cacttgtttc aaaattcaat cggtagctac gaatatcttg atttgctgaa cctaccatgc
      421 agatttcatc atccattatc aatgtttttg catggaggaa gccgttctca tagacaagga
      481 tttgtacatt ttccttgatc agctgccgag cataatattg tgttgctcga taaataaaag
      541 gatgatccgg tttgttggga atcatgattt tcacatctac accagaggca gttgcgattt
      601 ttaaagcagc aatgacactt tcatcaggaa caaggtaagg tgtctgtatc caaacacgtt
      661 ttttagcaga agtaatcaat ttgataaatg aaagcttgat ttgttccctt tcgttattcg
      721 gtccgcttga aacaagctgg atggatgtat cagcaagctt agattcatct cgatctgctt
      781 ttttaaagaa ataattcaaa tgatagccga cctttttttc ttcaggtacc gagacgttcc
      841 agtctgttaa gaagcgaagc tggagcaaag aagaagcggc accgaaaatc cgtatatgcg
      901 tatcgcgcca atagccgaat ttttttgttg tatttacata ttgattggca atattgaaac
      961 caccggtata actaatcttt ccatcgatca caacgatttt tcggtgatca tggtaattca
     1021 atcggaaacg aagtaatgcc ctttgtgagg taacaaatgt atggacatgt ccaccatttt
     1081 tgattagacg attgaaatct tttgcttttg tgccatgaga gccaaatgca tcatataata
     1141 ttcgaacttc cacgccttct gcggcttttt cttctaaaac atgtaagact tgctggccga
     1201 tattatctgt cacaaatgcg taatattcga tatggatcga atgtttggct ttttttagat
     1261 cttgaagcag tgaatccaat ttctcttgtc cgtctgtgag aagagtgaca gaattcattc
     1321 ttgtcagcgg catacgattt aatgaagaga agaagtcaac aaattgctgt ttgtctttat
     1381 cgcccatgtc aggatcgtaa tgttcaatgg tatctcctct aaaagactga aagttttcta
     1441 attctttcaa gtcactttgt cggagataga attttttctt atccgttaat ccacgtccga
     1501 aaaataaata taatacaaag cccaccccag gaagggcaaa caatactaag agccatgccc
     1561 aaatggctgc tacatctcgg ggtttgatca aaatagccac caaagcaata agtgcattta
     1621 atagataaag ggcggttata atactagata ccacaaaaat gttcctcctt acatacaata
     1681 acattagtat agcatgatag aagaaagaag cctaccaagg tgaatatact tttcactcct
     1741 ttcatgaaaa tgtaggtaaa tcgggctaaa gtagttgctt tttgtaggtt aataaggtag
     1801 gatattagta cagttgaata tctccttagg ggagtaaccg gcaacgtttg ttgcgataat
     1861 atcaacagta agttaaccaa atggttttct ggtattgtat gaaatggtga gacctaagac
     1921 aaatgcgtat ttgtcttagg tctctttttt ttcagacagg actagggcca agatcaaaaa
     1981 atgggggatg aaatttggaa aacaaggaac aagcacaaca agcggtctat aaagaaacga
     2041 tcgactcgtt gatgcagcaa acgcagagct caaccgatgg attgagcacc acgcaagcga
     2101 aggaaaaatt agaaagagat ggcttgaacc agctaaaaga aaagccgaag aagccaactt
     2161 ggaaattgtt cttagaaact ttcaaagatg caatggttat cgtattgctg attgtcgcac
     2221 tgattcagat ggtcatgggt gactacatcg aattcttagt tatttttgca gtattgatgt
     2281 tgaattctat aatcagtgtt gtccaaacaa aaaaagcaga aggttcttta gaagcgttga
     2341 aaaacctttc agcgcccgat gctcgagtga ttcgtgatgg taaggaaaaa acgattccgg
     2401 caaacgaact tgtggtagga gatatcgtgt tgttagacgc aggcgattat gtacctgctg
     2461 atggacgttt actagatgcg ggatcattaa aagtagatga aggtatgctg acaggagagt
     2521 cgacttctgc agaaaaagaa gtaacggata tcacaagtac ggtaccaatc ggagatcgta
     2581 caaatatggt attcagtgga acgatcgtaa cttacggacg cggtaaattc ttagtgactt
     2641 caactgggaa taatacggag atcggacaag ttgctggcct tttggaatca acaactgagc
     2701 aagtcactcc gttacaacga ggtctggata aattcagtaa aaaactaagt ttggctattt
     2761 tggcattgtc tttattgatt ttaggtatcc aactgtttcg tatttatctt ggagaaggta
     2821 caggagatat gactgcagat atcgtcagcg cagtgatgtt tgccgtagcc gtagccgtag
     2881 ctgctattcc agaagcatta cagtctatcg taacaatcgt gttatctttg ggaacgaata
     2941 aaatggcaaa acgtcatgcc attattcgga agcttccagc agttgaaaca cttggttcga
     3001 caagtatcat ctgtacagat aagactggta cattgacaca aaacaaaatg acagttgttg
     3061 actacttttt aggtaatggc gatactgggg atttcacaaa tgacccttca aaatggacag
     3121 ctgacgaacg tcgcttgatc gaaatcagtg ttttagcgaa tgatgcatcg atcagtgaag
     3181 acggtactaa attgggcgac cctacggaag tagcgtttat cgactttagt gaaaaattaa
     3241 accagcctta tcaggaaatc cgaaacaact acccaagaca agcagaactt ccttttgact
     3301 cagatcgaaa attaatgtct actggacaca caattgataa caaaaattac ttgtttgtta
     3361 aaggcggacc agatatcgtc ttccaacgca gtaaaaaagt attgctaaat ggtgaagtcg
     3421 ttccgatgac ggatgaattg atgaaaaaat tccaagagca aaatgaagct ttttcaacac
     3481 gcgcattacg tgtactagct tttgcttacc gtccacttga taattttgat ttatcatttg
     3541 aagatgaaga cgatttgatc cttgttggtt tgcttgctat gatcgatcct ccacgtgctg
     3601 aagtgactca ggcggtaaca gaagcaagaa gtgcaggaat caaaacaatc atgattactg
     3661 gtgaccacaa aacaacagct gttgctatcg caaaacaaat cgggattttt gaagaaggaa
     3721 acctggcttt gaccgggacg gaactagatg aattgacaga agatcaacta aacgaagttt
     3781 tagaaaaagt cactgtttat gcacgtgttt cacctgagaa taaaattcgt atcgttcgtg
     3841 cttggcaaaa taaaggtcat atctcagcta tgacaggtga tggtgtaaat gacgcaccag
     3901 cattgaaaca agcagacatc ggaattgcta tgggaacggg tacagatgtg gcaaaagatg
     3961 cagcagcgat ggtattgacg gatgataact ttgcttctat tgtcagcgca gtagaagtag
     4021 gacgtaatgt gtttgataat atcaagaaag ctatttctta tctatttgca ggaaatttag
     4081 gtgcgatcat tgcgatcatc tttgccttag ttgctgactg gtccaatcca tttacagcat
     4141 tacaactgtt gttcatcaat ttagtcaatg attcactgcc agcaattgca ttaggtacag
     4201 aaaaagctga acctgctacg atgaaccgcc cgccaagaga tccaaattca ggaatcttta
     4261 cggacaaaac attgatttct gtaacgtatc gtggtgtgct gattgggatt gctgttatct
     4321 ttgctcaatg gcttggcatg cagcattcac ctgaattcgg tgtagccatg tcattttcaa
     4381 cattgatttg gtgtcggaca ttgcaaacat tcccagcacg ttcaaacaca caaacagcta
     4441 tccaagcagg gttcttctct aacagaagtg tcttactagc tgtagttgta tgtagcgcgc
     4501 tttactgctt aacaatgatc ccaggactga aagaagtctt ctctatccca tcagcatttg
     4561 gattgacaga agtctttaca tgtatgggaa tcgcacttgt gacaattgtt ttgatggaaa
     4621 tcactaaatt gatcattgta cgagtacaag gaaataaata aaacgaatca acgaggttgt
     4681 gagcctgccg tttaattctg agtcacaagg tgcaatttca acaaacagct tctcatgttt
     4741 gttgaatatt gtgacttgtg accgaagagt tggcaggaga acaccgttta aggaagaagt
     4801 tgtaaaatca gcattttgag ctaatactga gggtcatctg attggacact gtttattcgg
     4861 aaagagggac tttgacacga ctttggtcac agtccctttt tttatttatg atagacggaa
     4921 agccaatcat tttattattc atcaaaagag atagaataga taaaagagga gaatgattta
     4981 ttccgaaaag tgaaaacata taacaaaaaa ttagaggaaa tcaaatgaat gaaaaagaat
     5041 gggatgaatt tgctgaagaa tattatgaaa tccagcaaga atcgcaagta actattgctg
     5101 aagatgtaaa aaattatctg aaaacggaga aagtcctgcc tgctgaaaca gcagcagacg
     5161 ttgctggagg atctggcgga tttctttcct tagcaaaaga aattaatcgc tatgatttga
     5221 tcgatttttc ttcaggaatg ctagaaaatg caaaaaaaga agcagaaaaa aatgagttgc
     5281 caaatgtttc ctttatcaaa cagtcgttag aagaatttat ccaatcaaat gagacatatg
     5341 aaatggtttt tacagcagca aatccagcat tggtacatct tattcaatta gaaaaattga
     5401 tgaaaaagtc aagcaaatgg tgcgtgataa tcagagtagt ggaatcaatc gatgatgttt
     5461 tttatcctat cgaaaagcaa ctaggaatga tagaagaaga tcctaataca tcgccagttg
     5521 taatggatca atttgaaaca tacttaattg atgaaggatt caaagtgaag aaaaaagatt
     5581 ttacttattt tacacaggaa tcaattactg aagaattgat gaagctatac tatgacgcgt
     5641 acgcaaaaga tcctgttgtg accaggtaca ttcgcaaact attcagtgaa aaacgagaaa
     5701 tccttagtac gactaagctg acctatagac tgctcctttg gtccgcagat aaaaaaagaa
     5761 cctttcacga ctcgtgaagg gttcttttta tgattttatt atattttgta gtaacgaata
     5821 tcattttcga tctttgtaat cgaaaaaggt acatctgaag tatattctgc acattctccg
     5881 tgttcaggaa gcgtctcact gaaaatcgtt tcatattcgt caacagtgat ccgtgttcga
     5941 gaatctagca tctcttgatg agattgtgcg aagagatatt cttgatagtt ttcttccaga
     6001 taacctgtaa aaaattcact tactgcacca gaaccgtagc tgaatagacc gatgcgatct
     6061 cctggctgta gtgatttcga attttccagt agagaagtta gccccaggta taatgaacca
     6121 gtgtaaagat taccgattcg tcggctgtaa cggatgcttt cttcatagcg agccataaga
     6181 cgttcctgat tttcttcgtc tgtttggtct aatacgcttt gcaatgcctt ttttcccatc
     6241 ttagtatacg gaatatggaa agcaatcgcc tgataatctt cgagtcctcg acccgacaat
     6301 tctttatgtc gattccaaac tttttggaat gattcgatat acgtagaatt agataaagga
     6361 ccatcaacaa caggaaattc gctataatct ggacgccaga aatcatagat atcttctgtc
     6421 agaaatacgc tgtcgtcttc aatcgataaa atacgcgggt tttgagtgat catcatcgca
     6481 acagcaccga caccttgcgt cacttcacca ccgcttgcca agccgtaacg agcaatatca
     6541 cttgctatga ctagtacttt tcgttctgga tgatttttga catattcttt cgccatgtgc
     6601 aaagctgcgg tgccaccgta gcaagcctct tttacttcaa aagaacgagc aaaagattgg
     6661 attttcagta aatggtggat aattaccgca ctcgcttttg aatgatcaat cccggattct
     6721 gttccgacga tgaccatgtc gattagctca cggtcttttt ctgttacgat tttgctggca
     6781 gcatttgctc ctagtgtaat gatgtcttca tttgcacgat tgactgccat ttggtcttga
     6841 ccaatcccta tatggtattt tgcaggatca tccccacggc tttctgccaa ctctgtcata
     6901 tctaaatata aattaggaat aaaaaaggaa agacgatcaa tccctatttt catttcaaca
     6961 cctctttact tatgataaaa aacttacttt actaaggtat cataaaaaga ttaaaagaaa
     7021 tataaaaaag agaatattta aggattgtta aaatttaatc ttagaaaaaa ataactggaa
     7081 caagagctat ttgtttttac agcaaagaat ggtatatttt tgtaggaggt ggcttacttg
     7141 aaagaagtcg ttatgataga tgctgcaaga acgccgatag gaaagtatcg cggaagttta
     7201 agtccattta ctgcagttga attaggcaca cttgtgacta aaggattatt agataaaaca
     7261 aaactgaaaa aagataaaat cgatcaagta atatttggaa atgtcctgca agctggtaat
     7321 ggtcaaaatg ttgccagaca gattgctttg aacagcggtc tccctgtaga tgtcccagca
     7381 atgacgatca atgaagtttg cggatctggt atgaaagcag tgatcttagc acgccagctg
     7441 atccaattag gtgaagcaga acttgtgata gccggcggaa cagagagtat gtctcaagca
     7501 cctatgctga aacagtatca gtcagaaaca aatgaatatg gtgaaccaat ttccagtatg
     7561 gtcaacgacg gattgactga cgcattttca aatgcacata tgggattaac cgcagagaat
     7621 gttgcaacac aattttctgt gagcagagaa gagcaggatc gctatgcctt gtcgtcccag
     7681 ttgaaagcag cacatgctgt cgaagccggt gtattttctg aggagatcat cccagtcaag
     7741 atttctgatg aagacgtgtt atctgaggat gaagcagttc gtggaaatag tacattggaa
     7801 aaactgggca cgttacgtac agtattctca gaagaaggaa ctgtaacagc aggaaatgct
     7861 tccccgttga atgacggtgc ctctgtggtg atccttgcat ccaaagaata cgcagaaaat
     7921 aataatctgc cttatttagc aaccatcaaa gaagtagcgg aggtcggtat tgacccaagt
     7981 atcatgggaa tcgctccaat caaagccatt caaaaattga cagatagatc aggaatgaat
     8041 ttatcaacaa tcgatctttt tgagatcaat gaagcatttg cagcttcttc tatcgtagtt
     8101 agccaagaac tgcagttaga tgaagaaaag gtcaatatat atggaggagc aatcgcatta
     8161 ggccatccaa ttggtgcaag cggtgcacgt atcttgacta cattagctta cggattgctt
     8221 cgtgaacaga agagatacgg tattgcttca ttatgtattg gtggaggttt aggcttggca
     8281 gtcttgttag aagcgaatat ggagcaaaca cataaggatg tgcaaaaaaa aaagttttat
     8341 cagcttacgc catctgaacg gcgcagtcaa ttaatagaaa aaaatgtatt gacacaagaa
     8401 actgcgttga tttttcagga acaaacactt tctgaagagc tctccgatca catgatcgaa
     8461 aaccaagtga gtgaagtgga aatcccaatg gggatcgcgc agaattttca aatcaatggc
     8521 aagaaaaaat ggattccgat ggcaacagag gaaccatctg tcattgcagc tgcaagtaat
     8581 ggtgcaaaaa tctgtggaaa tatctgcgca gaaacacctc agcggctgat gcgagggcaa
     8641 atcgttctat caggaaaatc ggagtatcaa gcagtgatca atgcagttaa tcatcgaaaa
     8701 gaagagctga ttctttgtgc aaatgaaagt tatccttcaa tcgtgaaacg cgggggcggt
     8761 gttcaagata tctctactcg ggaattcatg gggagtttcc atgcatactt atctattgat
     8821 tttttagtgg atgtaaaaga tgcaatgggc gctaacatga tcaactcgat tcttgagagt
     8881 gtggcaaaca agttacggga gtggttccct gaagaagaga ttctattcag tattttaagt
     8941 aattttgcta cagaatcatt ggcttctgcc tgttgtgaaa tcccattcga acgcttaggg
     9001 agaaacaaag aaatcggtga acaaattgcc aaaaaaatcc aacaggcagg ggaatatgcg
     9061 aagcttgacc cttaccgtgc agcaacgcac aacaaaggga tcatgaatgg tatagaagct
     9121 gtagtggcag ctacaggaaa tgatacacgt gcggttagcg cttcgatcca cgcctacgcg
     9181 gcaagaaatg gattatacca aggattaaca gactggcaga tcaaagggga taaactagtt
     9241 ggaaaactaa cggtgccttt ggctgttgcc acagtaggcg gagcttccaa tatcctgcca
     9301 aaagcaaaag catctttagc tatgttagat attgattcag ccaaggaact agcacaagta
     9361 attgctgcag tagggttagc tcaaaacctg gctgctttgc gtgcattagt tacagaagga
     9421 atccaaaaag gccacatggg acttcaagct cgttcattag cgatctcaat tggggctatt
     9481 ggtgaggaaa tcgagcaagt tgcgaaaaaa ttacgtgaag cagaaaagat gaaccagcag
     9541 acagctatac aaatattgga aaaaatccgg gaaaaataaa aaagcaatat ctttagaaga
     9601 ataaagcaag atttacagtt attttattta ctgcagatct tgcttttttc tctatcagaa
     9661 aatatgttta ttattagaat tttttatagt taaggttagg catcttaaga aatcaggcaa
     9721 ctccaagata tcttcaggta gaagagaagt cgcctaacat tgtaggatca taggtgattt
     9781 gttggacagg tcaagctgat atttgagcct gatgttagaa ggaatttctt tttacttgct
     9841 gtagaaatta ttatatgact aaaaaggctg ggattttaaa tcccagcctt ttatgacaaa
     9901 tagaattttt atcgatcagt aatcgctgtc gctgcaaatt gttcgatcaa aggaatcaaa
     9961 gtcaatttga ctttgtattt ccgttcgagt gctttttcga taacgaactc attttcagta
    10021 gtcatagaaa tagttgctaa tttacgaggt tcatcagcat ttttttcatt aataactact
    10081 tcatgttcat tttgttctgt gtaaaattta ggatactcag caacggcttt tgaaaaatca
    10141 gcaaaactta aagcggcatt cgtatcagtt gagcgtgtat tttcagttgt gtccgctttt
    10201 ggtttttccg ttttttcttc tggcacactg actgtttctt ctgtaactgg tgtcgcaaca
    10261 gcttgcgcag ttggttcatc gtttttcaat cgttgatcat tccagtaaga cacgatttca
    10321 ataagaagat tgtcacggaa tcgttcgaaa atcccgtttt ctcctgaacg aattctcttt
    10381 aatacagtat tgaagttgtc atcgattggc acgttgatcc gtgtatcttc atttgttttc
    10441 ccagctaatc cgatcgttaa gatttgacgg tttttgggat caagcatgac ttgtaacggc
    10501 aaactataac gtcgcaagct ctcttcttct agagaacgga ataattgtcc tcttacacca
    10561 tccaatgaag gacggaattg atttaataat ttttgtttct gttttttctt catatgttct
    10621 ctccttcgct catacccttt cgattataag aaaaaaacag ccaaatgaaa aggagtaatg
    10681 caaaaaaact tgcgtatacg caaattacaa cagaaaaatt tgcgtatttt cccgtttttt
    10741 gaaagaaaaa taaaaaatgg aacggattat gtatatatac ataatccgtt ccattctttc
    10801 tattttttcg gtatcaaggc aaaaacaggt agttcaggtg aataaatgac ttcatgttcg
    10861 tgccagggtt tgaccattag cgaatgatcg aaccgttttt tcagttcttc gatgatttga
    10921 tcatgtgtcc agtttcttgc aggaaaatgt tggattttct ctgcatcgat caaaaagaca
    10981 aaatcggaat gaaaagtctg ggtaacttct tccgagagag aattcaagcg ttcgattgga
    11041 tccataaaat cacctttcga cttaaaattg ggtagtatct gggtcttttg ctttgccgac
    11101 tacaccatct aactgatcga tttgtttcat ttcttcttct gttaattcaa aatcaaataa
    11161 ttcagtattt tctttgattc tgcttggtgt cactgactta ggaagaggaa ggaattcgtg
    11221 ttgcagcgac caacgcaaag caacttgtgc aatcgtcttg tcatgtgctt cagcgatttg
    11281 tttcatttca ggcacatcga agattttacc agtgcctaat ggactatatg cttcgaggat
    11341 catatcatgt ttttttgcat actcaactac agttgtctgt aattcgcctg gtgcaaggaa
    11401 aacttgattg accatcggca taatttttgc tgtttttgct agtgtatcta gatggtgggg
    11461 taagaaattg ctcacaccga ttgctttgat tttacctgct tcatacagtt cttcaaatgc
    11521 tcgccatgta tctgcatttg cttgttccca attatcacga aaagcaacag ggtttggcca
    11581 gtggatcaaa aacagatcta aataatctgt ttggagcttc ttcaatgatt cttcaaaaga
    11641 agacatcaca agttcatagc tgtggtttgc attccataat tttgttgtca agaatatctc
    11701 ttctctagga attccacttt ccttaatcgc ttggccgacg ctttcttcat tcttgtatcc
    11761 ttgggcagta tcgatatggc gataacctgc agccagcgct tctttaacag aggaaacagc
    11821 gacatcccca tcaggtgttt gccatgtgcc gaatcctact accgggattt cgtaaccatt
    11881 tgatagacga taagtgcttg ttaatgaatt catactttca gctccttttt tattcgttca
    11941 actataagtg tagcgcattt ttcgaaaaaa agaatccatt ttgtttctac tcttgaaatt
    12001 tttgtttaaa agaaaatgat tttgattctg gtatttttga ttttttttcc gtaaactaag
    12061 acagaaggga atgagaaaat ggatatctat ttaaaaaaag caattctgca tattattgac
    12121 agagaaactg gttctccagt cttttcacaa aaagagctgg atctgacaaa agaatatatt
    12181 cgtgattttc ttcaaaagaa gatccaaaaa atctcttctg ctcaaacaaa aactggacaa
    12241 ctagcagaag aaagcatgat cgttgaatca cttaaaatcg ctcaagaaaa ttttgtggaa
    12301 gcaagtgaag caatcgttca gcggtggttc gatatctacc aagaaagtga agaagcacct
    12361 agtgcagatg tatttgttgt cttgtatgaa ttagatacag tgatgtactt atctttattg
    12421 aaagtcaatt atcgtgaggc ttacactcac tttgttgaag cagacgaagc agggatcgac
    12481 aaccgcttga ttttgaatcg agcaattcta ggggcgaaaa cacaaaaagc agatgaagca
    12541 ttcgctgtga atttgactga ttggacctac gaattaattg aaaaaaaata tgagttttct
    12601 ggcaaaaaag agctttattt ttctggaaga gtgatcgaaa gtgttcctgc accttcttta
    12661 gaagaaaatg taaaagtgat ccaaaaagta gccaagcatc ttggaaaaaa atttgagaca
    12721 gaagaatttg atatcatggc tgatttgaaa gaagctgtct atgatacgat agaagaaaaa
    12781 ggtcggttgg atcatgagat gattgcggaa aaagtcttca aggaaaacat cacggcaaaa
    12841 atggctttta aagaagaagt acaagagcaa ggatttgtcc cagaagcacc tcctgtaaaa
    12901 gaagtacaag aaatttctga gaaaaaattt ggaaaacaaa aattcaagct atcaaacggg
    12961 atcgaactga tcgtaccagt agatgtatat cgcaacccag atttgattga atttgtgaat
    13021 aatccggatg gtacgatatc cgtgatgatc aaaaatgtgg atgaagtatt gaatcgttta
    13081 taaaaatgac tagaatatta ggtgttcaag taccagtgat cagttaaatg aattgtgtca
    13141 agagaaggat cggaaaagac gaatataaca aaaaaagagc gtgagacaaa agtaaagaac
    13201 acttttgtct cacgctctaa acacgtataa acggcggaag cagaagcaac tccttcttat
    13261 ttcttggagt taaacgcttc tgtcccagcc tctttttatc taataaaatt ttaagaccat
    13321 ttactaatta tcgatttttt ctgccgcatt tttaggcact tcagaagcct ttatttcttc
    13381 ccagcttaat acacctttac gaggattgac tttgagttta agatacgcat tcatctttaa
    13441 tggatgttct ctttcttcgc gaagcgtcac ctgttttttt tcaccatctt tattataagc
    13501 cgcttgcttg taggtataag tcgtgaattt ctctccgtta tctgcttggg tagctgacat
    13561 ttcgccattc gtcgtgattt ttgtataata agcttcgccg ccgtaaaaat aatgataagc
    13621 tgcaaaactt ccgccgagaa atatgcctaa aacgacaagt acgccaatta attttttcat
    13681 gtacttcacc tctttcctta ttattaacga aatagaagat ttcgtctatt gaaagttgtt
    13741 ggcaacagct gaattttaaa cttttcctca ttttgttctt attgcttcaa aagaaagtta
    13801 aggactatct tgaagttttt ctcaaaaaac acattttttt atttataaaa ggtacttttt
    13861 tcgcgtataa gacagacgag tgctcttagt tgactttgac caatattgac gtataataag
    13921 cttagttcga atcttacaaa aagtaagtcg gacgtattct ttttgattta gtcaaaaaaa
    13981 gactataata aaaaggtgag agctgaaatt tttcagtgat ctatattatt taggaggttt
    14041 gtaaattatg gcaaaagaac attatgacag aagtaaacca cacgttaaca ttggtacgat
    14101 tggacacgtt gaccatggga aaactacttt aactgctgcg atcactacag tattaggcaa
    14161 aaaaggccta gcaaatcctc aagactatgc tagtattgat gcagctccag aagaacgtga
    14221 acgtgggatt acgatcaata ccgcacacgt agaatatgaa acagaaaaac gccactacgc
    14281 gcatattgat gcaccaggac acgcggacta tgtgaaaaac atgattacag gggcagcaca
    14341 aatggatggt gcaatcttag ttgtttctgc aactgacggt ccgatgcctc aaacacgtga
    14401 acacatttta ttgtcacgcc aagttggtgt aaaatacctg attgttttct tgaacaaagt
    14461 tgatttagtc gatgatgaag aattgatcga tttggtagaa atggaagttc gcgagttatt
    14521 gagcgaatat ggtttcccag gcgatgacac tcctgtgatc aaaggttccg cattaaaagc
    14581 attgcaaggc gatccagatg ctgaagctgc tattatggaa ttgatggata cagtagatga
    14641 atatatccca acaccagaac gtgatacaga taaaccatta ctattgccag tggaagacgt
    14701 cttctcaatt acaggtcgag gaactgttgc ctcaggtcgt attgatcgtg gtgctgttcg
    14761 tgtcggtgat gaggtagaga tcgtagggat caaacctgaa acacaaaaag cagttgtaac
    14821 aggtgtagaa atgttccgta aaacgttaga ttacggggaa gctggggata acgtaggcgt
    14881 gttgttacgg gggatccaac gtgacgatat cgaacgtgga caagtacttg ctaaaccagg
    14941 ttccattact ccacatacaa aattcaaagc agaagtgtac gtgttgacaa aagaagaagg
    15001 tggacgtcat actccattct tcaacaacta tcgtccacag ttctacttcc gcacaactga
    15061 tgttacagga acaatcacat tgccagaaga tacagaaatg gtcatgcctg gtgacaacgt
    15121 gacgattgat gttgacttga tccatccaat cgctgttgaa aacggaacga cattctcaat
    15181 tcgtgaaggc ggacggacag taggttcagg tatcgttaca gagatcgaag cataagaaac
    15241 gcgactcgaa tattttggta atgaaaaatg tttaaaataa aagagcggtc caaacaggat
    15301 ttctgtttgg accgctcttt gttcttcaat tgtataagaa gagattgtag aagcatgaac
    15361 tttcgtttta cttcagtctc ttttattcat catgagagtg ttcatctatc ggtatgcagt
    15421 ttttaatact ttcttcgtat ccattttttc aaaccactta ttctaccttg tgtctgctca
    15481 actggcgcat tatcaataat cagcaaagca atcataacat atgcaatcca actatctcta
    15541 gaatatagag tataacgtga gacccagttt gtagcgtact tgcttttata ttctcttaat
    15601 ccatggaaag aataaaaatg agaaccaaac tcgtaaacca agtatgcaat cctttcctgt
    15661 aaaaaactct ttctggaagt tccgacattt gaaagagggg ccatccctaa attgaaccat
    15721 tggatatgtt cttctttcat ataggcaaac aaatgcaaga acaagaaatc catactgcca
    15781 gaaggtgctt ttttagaatc atatcgcatc agatcaatcg ttcctacttt attgttcgtg
    15841 taagtaggca tgatcgttgc aaaagcgaca atttcttctt gggtattttt gacaactgca
    15901 ataggcgctc tttgtagata atcttcagag aaaaagccta aagaaaaccc tttttccttt
    15961 cgtgaaccca accaattatc agagatattt ttgaaagtac tcatttgctc agtagaaaat
    16021 ggtggttgta agacatcaaa tgtaaatcct tctcgttcga tccgattcaa gacagcacga
    16081 gtgcccttca tttttttccc tgaagttgta aaactattca ggtctacata agcttcttcc
    16141 cccatcttaa tgaaatcgta gccgaattca tgcaaaatca taacaatctc ttcgcttgtt
    16201 tcgtagaaaa ctggtaaata gcatagtcga tctgtttctt cgataaaagc ttcgattgct
    16261 tctggaaaat cttctttctt accagaagga tcacccatta caatacattt attattgaag
    16321 caagcgaatt ggagaagaac agttggttca ccgtcttttt cataggcaaa catccgctta
    16381 tcttttaaga agatcaattg actatcggag tttcctccat aagttgtaag gatcttcagg
    16441 gccttttctt cattaaaggc ttcgccaatc tgtttctttt ctccttgaag gaaatgaaca
    16501 aatagaacga tcatgaaact aacagcgatg atggccaaca atcccgaaaa ccaaatcttt
    16561 tcagaaggaa ataagaaaaa ggaaataaaa tggtgacgcc gatgagggaa atccggcaga
    16621 ttgtataccc caataacgat gtaaagcaag gacagtgcac caataatgat tccatcgatc
    16681 gtacgccact cccatgaata gatcaattgt tcccgaaata attcgttttt agaagcaaga
    16741 atgatcagaa gcagaataga caagaaaacc accggtgtga aactaaaatc acttaataaa
    16801 acataaagta aagctagaac aatcaagaag attgtaggca aatatgctcg tttcacacgt
    16861 gctgcgatcc ctctgcccat aatgattaaa agaaatccca atagtatact aggaaattgg
    16921 ataataaaat gaaatttcaa tgggttcaaa ctatgaagcc agcgaaactc tgtaaaagct
    16981 tgcgggatag tggcagaaag tactagcatg attccagaaa agtataataa gacaaccacg
    17041 attttatgcg caatttctgt tgctaattgt ttagggatac cagaataacg ctgatcaaaa
    17101 gtggaaccga tatttttgaa gaaaaagacg attccaatca aaaacggaat gatgtagtag
    17161 aacaggcgat agagcaagat ccacacaacg actatttctc ttggcacacc aatagcggac
    17221 aacccgataa tcatcattac atcgaaactc ccaatttctc ctggaatcat agacacgatt
    17281 cctatcactg aagcagcaat gaataatgga attgtttgcc acagatcaat ccggatttcc
    17341 attaaataac caacagaaag gaaactaaac aggacaccgg tccattccaa caatgaaact
    17401 agcaatagct gtccttgagt tttaagtgag gtaccaccaa ggaatccttt ctttttgaaa
    17461 gtagtaaata aaaagacgac aggaaaatat agaccgccgc cgatcaacca tatccaatat
    17521 tgcttcaaaa aaggagcaac tggcgtgaag actacaagta aaaaagaaag cagactgtaa
    17581 atagaaagtc cagccatcaa aaacaagaaa actttcgata atgcttggat cacgtttctg
    17641 ctctctttct tttgtccata tagttcagag cgaagcccca cactaacaaa cccgccgaaa
    17701 ccggcaatat tgttgatcgt attgatcagc cagcttgtct caaacaggta acgaggattc
    17761 tgcttttgtt caagcatccg attcaaaata atatcataac caaccatagg taacacagag
    17821 ataagaccaa tcaatagcat tagtccgctt ttccatacag gtatagtggc aaacgtttct
    17881 cctaattgct ggatagatag agttttaccg atggacatca attcaccgac aataatgaca
    17941 atgacagaaa tcaaaaagat tgttttgaac agtcctaggt ggtttttcag ccattgtatg
    18001 gtatttttta acaaataaat caccttctat tcctaatagt tatgtttcag aaagaaattg
    18061 aagaacttct cgattgaagc gttcaggatc ttttctagct aattcatgtc cttgttcttt
    18121 cacaagcaca aaggaagctt ttggtatagt tttagctata tacaaagaat gttttagctt
    18181 gattacatct ttttttccca cgatgatcaa agtgggggag ttgatttttt ttaaatcatt
    18241 ttctgtaagc ccgatgtcat gcagcaaaag tttgataact aaaagatttt tgcgaagact
    18301 tgggcgaaat aaagagagta accataccca tgcataatga atattactga tgactctggc
    18361 agaaaaacga acgcctttaa caagcgtgtt tccagaattt aaaatcaatc gatgaacttt
    18421 tttagggaag ctgctggcaa ataccagtgc tagatttgct ccatcactaa atccaagaaa
    18481 atctgcctga tcgatttttt ccagcaacat gatcgtattc agatcctcgg ccattaactg
    18541 aaaattcaac atagatgctt cgtttgtcga acgaccatgt cctcggctat ctactagata
    18601 cacggtatag tagcgttcga gtacagggac ctgttctgaa aaaaagcggc cgctgccgtc
    18661 attgccatgc agtaaaaaga gagggaagcc ttgaccgctc ttttcatagt aaatttcgct
    18721 cccatctggc atttttgcat atttcttttc ttttttcaca taatcaccta ctgtgtaaag
    18781 tataacgatt atatcagtca ttgggcaatt gtttttactg aaaaacaacg atcattttcg
    18841 taagatgtca aaaaatacag gaaaattcat tttttcttaa ggcgtacaca gaaatcaagg
    18901 aagaaatagc caagaagata aagaaatagt aagataaaaa aagatgtcaa aataactagg
    18961 tgatgaaatg aagcgaaaac gattatctat tatccgtatt cttttttaca tgatcattct
    19021 gacggttatt ggtgcggtgt tgtttttatg aggaagaact tatcgttcgg taaaacgagt
    19081 ggaaagctat caaatagaaa taacaacagc tgcaaaaaat tatcatatgg aaaactacga
    19141 gaatttgatc aaggcgataa tattgaccga atcaaaagga aaagggacag atctcatgca
    19201 gagctcggaa agtctgcatg gagaagctga gatcattgat aatccagctg acagtattga
    19261 tcaaggtgtc aaatatttgg cggaaatgat agtagaagca aagaaacaag gatgcgattt
    19321 atggacagct gtccaagcat ataattttgg gaaagattat attgcctatg taaaaaatca
    19381 tggcggagaa aacacagtag aattagcaga agcgtattct aaagatgtgc tgagcccact
    19441 tttaggaaat gaggaaggga aacaataccg atattggcgg atccagtcct tgctttacaa
    19501 tggcggctat ctctatcata atggaggtaa catgttttat cctgatattg tcaaaatgaa
    19561 tgaaaaaata atagagacct atgaaaaagt ctttaagtaa aatagcagga aatgttcaat
    19621 catgaataaa attaaatgaa agaaggagtg cgcaaatagt ggaaaaaagg caaaaaatgg
    19681 aaaatcaaga agtggggtac acaaccaaag actggatgtt acggtttgtg aagggaatgt
    19741 ttatcggttc aggatttatc ttgccaggag tcagcggtgg tgcattagca gctgtctttg
    19801 gcatgtatga gcggctgatt tcttttttag cccaccttac aaaaaatttc aaagaaaatg
    19861 ttttgttttt tctgcctgtt ggtctaggcg gagtgacagg catctttcta ctttcttttt
    19921 tagtcagttt tttattaggt gcgtatgaaa caacgatatt atggtttttt gtcggatgta
    19981 tcgctggaac tgttcctgcg ttatggaaag aagcagggaa aaaaggaaga gatacttctg
    20041 atgtgatcat catggttgtt acttttgttc tggcttattt gtttttatta tacggcgctc
    20101 aattatttga cgggcaagtc gatcagaatt ttggaacatg gctgattgcc ggcggattga
    20161 ttggtttggg tatgatcgtc ccaggattga gcccttctaa ttttttggtg tatatgggat
    20221 tgtataaagc gatggctgac ggatttaaag aagtacgact tgaagtgatt gtgccgatcg
    20281 gaattggtgg gattctttgt gttcttggat tgtcaaaaat catggattac attttctcaa
    20341 aggcatatag caagctattc catttcattt taggtgtagt ttttgcttca acgattatga
    20401 tcattcctac cgattacgcg ggattgggag ttgctggggt actagcttgt cctgttcttt
    20461 ttatagccgg tacagcacta ggttggtgga tgagccgttt ggaggaacaa tataagtaaa
    20521 gatagattga taataaaaac agcacacggg tgattcaaac aaaaaggttg gcatcttttt
    20581 tgtaaaatca ttcgtgtgtt tttctataaa ttttataaaa acaagttgtt tttaatcttg
    20641 gaacacgcta gacttaaaca cgtgattatt tttgaaagtt ggcggaaaat ggagaaaaag
    20701 aaaatgaatg gacttacaca gcaagaggta gttcagaaaa tggcttctgg ccagcaaaat
    20761 gactaccagg aagatgcatc aaaaagtaca aaacaaatat tcagcgataa tatattgacg
    20821 ttattcaatt ttctgaactt tggtattggg atttgtctgt tatttgtagg tgcgtattcc
    20881 aacatggcgt atttagcaat tattgtcgtc aatatcgtta ttggaattta tcaagaaata
    20941 catgcaagaa atcttgtacg gaaattatcg attgtttccc aatcagaagt actagtgatc
    21001 cgggacggta aagaacaatc gatttctact aaagagctgg tattaggaga tttggtaaaa
    21061 atcggagcag gagaacagat accagccgat ttacaggtta tatctggacg ggcagaagca
    21121 aatgaagctc ttttgacagg cgaatctgat ttgattgtca aagaaactgg agctgaattg
    21181 ctttctggaa gctttttgac aagcggttct gtcatggctg aagttatccg agtaggtgca
    21241 gataattatg ctgtccgctt gactagcgag gcaaagactc acaaagctat tcgttctgaa
    21301 cttgtagatt cgattcgcaa agtatccaaa ttcactagtt tcgtgattat tcctttgggg
    21361 atcatactat ttttggaagc actgttcatc cgcgatgctt ctatcaaaat ttctgttatt
    21421 gcatctgcag cagctttgtt aggcatgctt ccaaagggtt tggtattgct aataagtatt
    21481 gcattaacga caggggtaac caaactggca aaaaaacgaa tccttgttca agacatgtat
    21541 tcaattgaaa cgctggccca tgtcgataca ttatgcttgg ataaaacagg gaccattact
    21601 gaagggaaaa tgagtatcca gcgggtagaa gcactacatc cgctgtacga gaaagccatc
    21661 ccagaaatta tggggacgta tctggcagaa agcagtgata acaacatcac tatgcgggct
    21721 atgagagatt attttgagct ttctaatcgg tatgatgtga caaacaaagt cgcattttct
    21781 tctgatcgca aatggggcgc gatggaattt ccagaactgg gagtcgtgta cgtaggagcg
    21841 cctgaacgtt tggtggaaga agatcggctt ccggacagtc taaaaacagc ccaagcgaat
    21901 ggctatcgcg tacttatgtt ggggatcgct ccaaatcagc aattgaacga agaaaaacca
    21961 gcatttgttg agccggttgc tctgtttgaa atcgatgatc caatccgaaa aaacgctcaa
    22021 gaaacactag cttatcttca tgaggaaggt gtggatctga aagtgatttc tggagacaat
    22081 ccttttactg tctccaatat cgctagacgt gcaggattac cgaattatga tgcgtatgtc
    22141 gatctttctc aaattactga agaaatggaa gtgcgagaag ctgctcatcg ctatacggtt
    22201 tttggacgag tgagtccgca gcaaaagaaa ttgctggtaa atgaattgaa agaaagcggc
    22261 agaacggttg caatgacagg cgacggtgtg aatgatgttt tggcgttaag agaagcagat
    22321 tgcagcattg ctatggcaga aggtgatgga gcaacacgtc agattgcgaa tctggtattg
    22381 ttggactctg attttactac attgccagaa gtcctgtttg aaggacgccg agtggtcaac
    22441 aatgtgacga aagtctcagg catctttttt atcaaaacga tttattcatt tattttatcg
    22501 attatatgtg ccgttacagc aattggattt ccgtttatcc ctatccaaat cacgctgatt
    22561 gatttagcga ttgaagggta tccttccttc tttttatctt ttgaacaaga taaaagaaaa
    22621 atcacttacc gttatttgcc tacagcactt attaatgctt taccaaatgc cttgcttgta
    22681 gtagtgaata tcattgcggt gtatctactt ggaaaaacat tcgcatgggg aaatgctgaa
    22741 actactactt tgatgtatta cttgctgatt gggatcagtt gcatggcagt tgtaaaagct
    22801 tgttatccct tcaacccatt gcgcatcttc ctagcagtaa cgacgatcgt agggatatat
    22861 gtagctgcaa tgctgttcca tggaattttg gaagtaaact tagggttggg agagagcgta
    22921 ggatacttct ttgtattcat gctcgtaaat attgttcttc gagtattgta ctggaaggct
    22981 gatattcaaa atcagttact gaaaagagta gcaaattgat gcttaatgag atagaaatgt
    23041 gttatacttg aaaaagcaat gaaatatgat acaatcaaac atgtataatt gtgtccttta
    23101 aaatcgcact gatttcagtt agtgcgattt tttaccggtt attctcctga aatatcttct
    23161 gttaaaccca aaagatatca atagagcagg gggtgaaaaa atggctcaac ggattcctca
    23221 agaggttatc gaaacagtac gcagtcaaac gaatatcgtt gaggtgattg gacaatatgt
    23281 ccagttgaaa aagtctggta aaaattactt ggggctgtgt ccttttcatg aagaacgtac
    23341 accgtctttt tctgttgcag aagacaagca gatttttcat tgctttggtt gtggtaaagg
    23401 cggaaacgtg tttacttttc ttcaagaact agaaggtcta agttttccag aagcagtggt
    23461 aaaagtggca gacttcgaac agataccgtt agaagatcaa tggcgtcagc gtactatagc
    23521 tgtccaaaat cctgaatctg ctacggggaa actgattgct gctcatgaaa aagcagcgga
    23581 aatctatcat cacatgctat tgcatacaaa agctggacag ccagctttag aatatttgca
    23641 aagtcgtggg ttgtctttag aattaattga agaattcaac ataggttttg cacctaatga
    23701 gcgttctttt cttcagcaag tattcaagaa tgaatcaatt tcaaatgaat tgttagaacg
    23761 ctctggattg tttgtcacgc atgatgatgg ccgtatgtct gatcgttttt atcaaagagt
    23821 gatgtttcca cttcgtaatt ctcaaggcca gacgatcggt ttttcaggac gctggcttgc
    23881 acctgaaaaa gggcaggaaa aagatcagcc caaatacttg aatagtccag aaacagaact
    23941 gttcaataaa agacaagttt tatttaatct tgataaagca agaagcgata tccggaaaaa
    24001 cggagaaatt ttgttgttcg aagggtttat ggatgtgatt gctgcatggc aggctggtat
    24061 caaaactgga ttagcttcta tggggacaag tctaacagct caacagatcc aacaaatgga
    24121 aaagctgacg aaagaattgg tcttttgcta tgacggagac aacgcaggtt ttgaagcaac
    24181 caatcgaggg attgaattgc ttcggcaaaa cagtcgatta gcactttctg tcgtagtcgt
    24241 tccagaaaag cttgatcctg atgaatatct ccgtaagtat ggagaagcat ctttccaaga
    24301 attagtggct catgggcgcg aaacagtctt tacttttaaa tcacgttatt taaaacaagg
    24361 gaaaaattta gataatgaaa aagacaagat cgaatacctc gaagaattac tagttgaact
    24421 tagccgagtt gtttcaccaa tcgaacaaga tatgtatcta tctcaactgt caacagagtt
    24481 tcagatcggc agagagacga tacaaaaaca actgcgcgat cttcgaagga caaatcagca
    24541 aaatcgtcaa gaacctcagg aaagtcaaca cactcaggtt acgagacaag aaactgcacg
    24601 gaaaaagaga cctttgacgc aagtagaaaa ggcagagcag attctattat atcgtgcatt
    24661 taaagaagtt tctgtccgta atattttaaa agaacgagaa tttcagttta ttcatgatgc
    24721 atatgcagaa gtttattttt tattcgatgc atatgtctca caacattatg aattcaacct
    24781 tgcagagttt ttagactttt tgcaagatga aaatttaagg cggttggtgg tggaaatcga
    24841 atatttaccg gtggcagaag aaagttcgcc acgagaaatc caagacttgc tagatgtgat
    24901 cagcaaatct ggcttagctg atgaaataac attaaaaaaa caaatgcagc aagaagccag
    24961 acgaactgga aacaaacagc ttgagcttga attgacgatt gaaatcatca atttaacgaa
    25021 acagttaaaa caagccaaat agctactcaa ggggggcctt cttttatggc aaatggaaca
    25081 gataaaaaaa cgtatgaagc agcagtagca gctttcatca aagaaaacaa acccaaacga
    25141 caagttgtat atgatgattt atccaatcag cttgctacac cattcacttt gaatgcagat
    25201 gaaatggaca aattgatcca aaagattgaa gacgcaggta tcagtgtggt ggatgaaaat
    25261 ggtgatccat caattcacag cttgaagagt gctgagaaaa aagctgaaca agcaaaaaca
    25321 gaagatcttt ctgcgccaac cggtgtaaaa atcaatgacc cggttcgtat gtatctgaaa
    25381 gaaattggcc gtgtctccct tttaactgcg gaagaagaag tagcgttagc attgaaaatc
    25441 gaagaaggcg accaagaagc gaaacagcgt ctggcagaag caaaccttcg tttagtagta
    25501 agtatcgcta aacgttatgt aggacgaggc atgcagttcc ttgacttgat ccaagaagga
    25561 aacatgggac tgatgaaggc agttgaaaaa tttgactacc gcaaaggatt taagttttca
    25621 acctatgcta catggtggat tcgtcaagcc atcactcgag caatcgctga ccaagcacgt
    25681 acgatccgta ttccagttca tatggttgaa acaattaata aattgatacg tatccaacgt
    25741 caattgctgc aagaccttgg aagagaacct acgccagaag aaatcggtgc ggaaatggat
    25801 cttccaacag aaaaagtccg agaaatcttg aaaatcgcgc aagaacctgt ttcccttgaa
    25861 acgccaatcg gtgaagaaga tgactctcac ttaggtgatt tcatagaaga ccaggaagca
    25921 acaagtccag cagaacacgc agcttatgag ttactaaaag aacaattgga agatgtacta
    25981 gatacattga ctgatcggga agagaatgtc cttcgcttac gttttggttt agatgatggc
    26041 cggacgagaa cattggaaga agtagggaaa gtatttggtg ttactcggga acgtattcgt
    26101 cagatcgagg ctaaagcatt aagaaaattg cgccatcctt ctcgttcaaa acaattaaaa
    26161 gatttcttag aataagcgtg gactattgac cagaagagaa atcagtaaaa ctgtttggtt
    26221 tattttaaaa gaaaaaaagc ttgagactac gcagtctcaa gctttttttt ctataggaag
    26281 gaaattcatt cattattttt aagtaaatcc taaaaataag ctattggcgt gacgtataga
    26341 atatgataga ttaaatggta gcgtaaacct agtaaaggag ccgatcgcta agtgatgaaa
    26401 aaatgtccaa attgcggatc gaaaaaaatg gagcagaata ctatctgtcc agactgcggt
    26461 catcaaataa ctgcagaaga tcagcaaata aaagaatcaa ttgaagaaac aaatacagat
    26521 gcagctatgc aggataaatc aactgaagat gcagtctttc ctgatacaga attaaacgat
    26581 ccaatcgaat ggtcggaatt gaaagattta cctctagaat ctgtaatgga attattcgaa
    26641 aaagcagaac cagaagacga agaaccacct gctcacgatc aagtaactaa tatagagaaa
    26701 aatgaacaga agaaagcaac tgttcatgag acttcggaag aatcaggaaa gaatcaagaa
    26761 gaaacgcttg aatcaaaaca aaaaagaaga gtagctgaac tgaaagaaat cgtcgataat
    26821 gaggaagaaa attcgatact atccgcttat atcaaagcac atcgtgaaga tacaaaagaa
    26881 gagcacgcga aagagctgct caaaatgatc agtgaaaaaa ttgcacagga aaacaaagta
    26941 gagccagaaa aggaacaaga aactgaaaaa gactctgata gagaaaacgc cacaggtaaa
    27001 gaaacagtaa aaataaaaga cgtaaaaacg gatatcacag aaaaaactga agtatttact
    27061 gaaaaacaat tagaaaagga aaaaacgaat aaactgccgg aagcagagaa aaaagaaata
    27121 ctcgaaaaca cagacaaaaa gtcaacagag cccgatgaaa taaatagtga atccgatgct
    27181 gaaaatcaat ctgtaagcaa agagggtact gatcttgaag caaaaataga aaaagaagag
    27241 aaccagccta agctgtttga agcagaagcg aaatcaataa caatcgtgcc agatactgaa
    27301 aaaacacatc aagaatcaaa cttagaaaaa acagatccag ataaagcgca aaagaaaaaa
    27361 tcaaaaaaag taccttatct cgttttagca gcagtacttc tactcggtgg aggaagctgg
    27421 gcttattatg accatcatca aaaagtagaa gccgaagtag cagcagaagc aaaaagacag
    27481 caacaaatgg aagaacttca tgagaaactt gctgcctttt atacagatga tcaccatcaa
    27541 tttattcgtg cagatatgat tcatcaagat ttatcaaaac taaaaaatga tttatccagt
    27601 cttaagaatg aaaaaggata ttcagagtta gaaacaactt accaagatat ccaatctaag
    27661 atccgagata tccaaaaagt gaatgagtgg ttcatagcac ctgccatcgt cgatgatcat
    27721 ctagcagatg acatcaagtt gaaagcagat aaagagatcc aagcaatcga aacggataac
    27781 acagattttg gaaaattgat tcaacaagca caaacagaag ctaaaaatca atatgctgaa
    27841 ttgcaaacag ctaaagaaaa aacagctata gtctttaaag acggaaaagt agtggattca
    27901 gcaacacgcg accaatacaa gacggcaaaa gaagcagtag aaaaagtgaa aaatacagat
    27961 ctatctgctc agttgaatga acaattgaaa caagtagata agatgctcac tgataaagag
    28021 aaaaaagaag aagaggaaaa gaaaaaagca gaagaagcga aaaaagcagc tgaagaacaa
    28081 gcaaaacaag ccgctcaagc agcagctgca gctcaagccg ctcaacaagc tgcggtcgca
    28141 caagcagcac agcaagaagc ggcatcctcg accccttcct cttcaaactc agcgaatcag
    28201 ccaatcaaat ctgccagagc aagtgatatt gccgatgcat ctaatccagc atggacgtgg
    28261 gcaccaggtg tccaaagcaa agtgattgct acatgtatcc aacgtggata tatcgttgaa
    28321 ggaggttaca gattagaaaa agccagaatc gaaaatgggg aaggctatta taatctatat
    28381 gccacttcaa caaaatctgc attgatgaaa ggcattggag aaagtgcttt gccattttac
    28441 atcgtaacca tcaactgtaa aactggatgg ttcggaggaa atgggagcaa ttaattttag
    28501 gcaaatgaaa gaggctgtga gcctgccgtt taacaccgtt tattcgaaaa aggggagtat
    28561 gacacaactg ttgtcatact ccttttttca cctttccaaa ccaaggctca gctattcaag
    28621 tagataacat tatgttatga tagcaaagaa atgtattaag aaaacctttc actatagagg
    28681 gagaaaaaaa tgaacgattt attagcaact atatttacag gtattattat tgatgaaaat
    28741 gaaaaaaact actttgtcca aaaaaacgga caaacctttg gcttaagcaa atcagaaggt
    28801 gaacacaaaa tcggtgaagc agttgaagga ttcggttacc aaaatcaaaa acatgaaaat
    28861 cggatcacta cacaaattcc taaaagccgt atcggtcatt acgcttttgg aatcgttact
    28921 gctacacgaa aagatttagg tgcgtttgtc gatatcggtc ttccagataa agactttgtc
    28981 gtatctcttg atgaattgcc gacaatgaca gagctatggc ctaaaaaagg ggatcgcttg
    29041 atgatttccc ttagagttga tgcaaaagac cgtatttggg gcagtttagc agatgaaaaa
    29101 atattcaaat ctttaagcaa atatggaaca gaagaaatga aaaacaaaga tgtgatagga
    29161 acagcatacc gcttgaaatt aacaggtacg tacgttttaa cggaagattt ctatcttggg
    29221 tttatccatc cttctgaacg atataatgaa cctcgtctag gcgagcagat aaaaggtcga
    29281 gtgattggtg tccgaccaga cggtgtactg aatctttcat tgaagccaag agcatatgaa
    29341 gctatatcag atgatgcaca aatgatttta accattttag aacgttctgc agatcagcaa
    29401 atcaacttta cagataaatc ggatccagaa gaaatcatga aagcttttgg tatcagcaaa
    29461 ggagcattta agcgcgcatt aggtaatctt ttgaaacaag gattgattac acaagaaaac
    29521 ggtgtgacaa aactagtcaa aaaatctaat tgagaatccc ttgcatgttt ttcttaacta
    29581 gcttataata attttaatta acatagtgtt atatcttttt ttatttgtaa ttattataaa
    29641 ttgttggggt tgagtaaatg gtcatgaatg ctatttcggc gatgaaaaaa acaaaacagc
    29701 agctacatga agcggggttt aagctgacac cgcaaagaga agctactctg cttgtgcttt
    29761 tggaaaatga gaaagagcat ttatctgcag aagaaatctt ctttttagtg aaaaaaaaga
    29821 gtccggaaat tggacttgct actgtttatc gaacccttga aatcttgaca gaattgaaaa
    29881 ttgtcgataa agtaagtttt aatgatggat tagcccgtta tgatttgaga aaagaaggcg
    29941 ccaaacattt ccatcatcat ttactatgct tgaactgcgg cacgatcgag gaagtagaag
    30001 aggatctttt acttgaagtg gagcaagtag tagaagaacg ttatcatttt ttagtaaaag
    30061 atcatcggct aacgttccat ggaatctgtc aggaatgcta tgaaaaagaa aaa
//
LOCUS       DACDYI010000037        29846 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.037, whole genome shotgun sequence.
ACCESSION   DACDYI010000037 DACDYI010000000
VERSION     DACDYI010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.037"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            2..550
                     /gene="hslV"
                     /locus_tag="IWM52_001517"
     CDS             2..550
                     /gene="hslV"
                     /locus_tag="IWM52_001517"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAP9973773.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            564..1964
                     /gene="hslU"
                     /locus_tag="IWM52_001518"
     CDS             564..1964
                     /gene="hslU"
                     /locus_tag="IWM52_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAP9973774.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1982..2773
                     /gene="codY"
                     /locus_tag="IWM52_001519"
     CDS             1982..2773
                     /gene="codY"
                     /locus_tag="IWM52_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAP9973775.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2855..3730
                     /locus_tag="IWM52_001520"
     CDS             2855..3730
                     /locus_tag="IWM52_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP9973776.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3862..4500)
                     /gene="plsY"
                     /locus_tag="IWM52_001521"
     CDS             complement(3862..4500)
                     /gene="plsY"
                     /locus_tag="IWM52_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAP9973777.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4627..5061
                     /locus_tag="IWM52_001522"
     CDS             4627..5061
                     /locus_tag="IWM52_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAP9973778.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5227..7272
                     /gene="parE"
                     /locus_tag="IWM52_001523"
     CDS             5227..7272
                     /gene="parE"
                     /locus_tag="IWM52_001523"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAP9973779.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7284..9734
                     /gene="parC"
                     /locus_tag="IWM52_001524"
     CDS             7284..9734
                     /gene="parC"
                     /locus_tag="IWM52_001524"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAP9973780.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10011..12242
                     /gene="pflB"
                     /locus_tag="IWM52_001525"
     CDS             10011..12242
                     /gene="pflB"
                     /locus_tag="IWM52_001525"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP9973781.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12486..13247
                     /gene="pflA"
                     /locus_tag="IWM52_001526"
     CDS             12486..13247
                     /gene="pflA"
                     /locus_tag="IWM52_001526"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAP9973782.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13332..14258
                     /locus_tag="IWM52_001527"
     CDS             13332..14258
                     /locus_tag="IWM52_001527"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAP9973783.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14440..15333
                     /locus_tag="IWM52_001528"
     CDS             14440..15333
                     /locus_tag="IWM52_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAP9973784.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15467..15592
                     /locus_tag="IWM52_001529"
     CDS             15467..15592
                     /locus_tag="IWM52_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9973785.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(15726..16358)
                     /locus_tag="IWM52_001530"
     CDS             complement(15726..16358)
                     /locus_tag="IWM52_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973786.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(16506..17048)
                     /locus_tag="IWM52_001531"
     CDS             complement(16506..17048)
                     /locus_tag="IWM52_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAP9973787.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            17183..17572
                     /locus_tag="IWM52_001532"
     CDS             17183..17572
                     /locus_tag="IWM52_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973788.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            17576..18340
                     /locus_tag="IWM52_001533"
     CDS             17576..18340
                     /locus_tag="IWM52_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9973789.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            18469..19416
                     /locus_tag="IWM52_001534"
     CDS             18469..19416
                     /locus_tag="IWM52_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9973790.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            19546..20286
                     /locus_tag="IWM52_001535"
     CDS             19546..20286
                     /locus_tag="IWM52_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973791.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(20577..21245)
                     /locus_tag="IWM52_001536"
     CDS             complement(20577..21245)
                     /locus_tag="IWM52_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9973792.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            21379..21666
                     /locus_tag="IWM52_001537"
     CDS             21379..21666
                     /locus_tag="IWM52_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973793.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            22082..23620
                     /locus_tag="IWM52_001538"
     CDS             22082..23620
                     /locus_tag="IWM52_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAP9973794.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            23617..24582
                     /locus_tag="IWM52_001539"
     CDS             23617..24582
                     /locus_tag="IWM52_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9973795.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            24569..25891
                     /locus_tag="IWM52_001540"
     CDS             24569..25891
                     /locus_tag="IWM52_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9973796.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      25954..26058
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            26132..26875
                     /locus_tag="IWM52_001541"
     CDS             26132..26875
                     /locus_tag="IWM52_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9973797.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            26890..27882
                     /locus_tag="IWM52_001542"
     CDS             26890..27882
                     /locus_tag="IWM52_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9973798.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            27894..28631
                     /locus_tag="IWM52_001543"
     CDS             27894..28631
                     /locus_tag="IWM52_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9973799.1"
                     /translation="MSIQISHLNVVIENLKVLEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            28621..29343
                     /locus_tag="IWM52_001544"
     CDS             28621..29343
                     /locus_tag="IWM52_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP9973800.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(29427..>29846)
                     /locus_tag="IWM52_001545"
     CDS             complement(29427..>29846)
                     /locus_tag="IWM52_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAP9973801.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        10260 a         5088 c         6288 g         8210 t
ORIGIN      
        1 catggtcgaa tcaccatttc attcaaccac tatctgtgcc gtcgaaaaag acgggaaatt
       61 tgctatggct ggagatggcc aagtaacaat gggtgaatct gttgtcatga aaggcactgc
      121 aaagaaagta cgacgtatct acaatggaga agttgttgta ggtttcgccg gaagtgttgc
      181 agatgcgttt actttagaag aaaaatttga aggcaaacta aacgaatata acgggaatct
      241 acaacgcgca gctgtcgaat tagctcaaga atggcggaca cagcaatcta tgcaaaaact
      301 tgaagcgatg ctgatcgtaa tgaatgataa agaaatgctt cttgtttcag gtacaggaga
      361 agttatcaca ccagatgatg gtatcttagc tatcggatca ggagggaatt ttgcgttggc
      421 tgcagcccga gcaatgaaaa actatgctca aaacgacatg agtgcgaaag aaattgcaga
      481 aaatgcgtta aatattgccg cagatatctg tatctttact aatcataata ttatcgtaga
      541 agaactgtaa gaggtgagta gatatgtatg aattaaaaca aacaccaaga caaatcgtaa
      601 gtgaattaga tgaatatatc gtaggacagc acgcagcaaa aaaatctgtt gccgttgctt
      661 tgcgtaaccg ctatcgccgt ttgcagttag atgaaaaaat gcaacaagat attacaccaa
      721 aaaatatgct gatgatcgga cctacagggg tcggtaaaac agaaatcgca cgacgtttgg
      781 caaaaatcgt caatgcacca tttgtcaaag tcgaagcaac aaaatttact gaagtcggtt
      841 atgtcggaag agatgtagaa tcaatggttc gggatttagt agaaaatgcg atccaaatcg
      901 tagaaaaaga gcagtacagc cgtgtataca caagtgctaa aaaaaatgca gaaaaacgtt
      961 tggtaaaaat cttggtacct ggtatcaaaa aagaacagaa acaatccact aatcaatttg
     1021 aacaaatgat gaatcttttt aatgctagtc agcaaaatac cgctgaaccg caagaagaag
     1081 tgacagaaga tattcgaatc aatcgccgta cagtcttgga acaactacaa aagggtttac
     1141 tggatagtcg tgaagtaacc attgaagttg aagaccaaaa gaaacctgca ccaatgatga
     1201 acaatggatt ggagcaaatg gggatcgacc taaatgaaac tctaggtgct ttgacaccga
     1261 agaaaaaagt ggaacggacg gtaacagtca aagaagcttt agagttattg atcaaggaag
     1321 aatcagcaaa attagtcaag gatgctgata tccatagtga ggcaatcaaa ttagcggaag
     1381 aaagtggaat tatttttatc gatgaaatcg ataagatcac ttctaaatca cagcaatctg
     1441 gagaagtatc cagagaaggc gtccaaagag acatattgcc aattgtcgaa ggttcacaag
     1501 taaacacaaa atacgggatc atccaaacag atcacgtcct attcattgct tcaggagctt
     1561 tccatttgtc taaaccaagt gatctaattc cagaacttca aggacgcttc ccgatacggg
     1621 tagaattaga tgatttgaca gctgaagatt ttgtgaagat tctgaccgaa ccaaataatg
     1681 ctttagtaaa acaatacgtc gcgttgatcg gtacagaaaa tgtgtcagtt acgtttacaa
     1741 aagaagcaat tgaacggatc gctcaaatcg ctttcgatgt caaccgtgac acagataata
     1801 tcggcgcacg cagactgcat accattttgg aaaaactact agaagatcta ttatttgaag
     1861 caccagatat gcaaatggga gaaataacga tcacagaagc atatgtggat gacaaacttg
     1921 gctcaattgc ggcaaatgaa gatttaagtc gctatatttt ataaaaaatc ggaggacgac
     1981 aatgacagac ttgctaacga aaacccgcaa gataaacaca cttttgcagc aaaagaatac
     2041 ttttgacatg caagcagatt tgccttacga taaaatggct gtcacactag gtggtattct
     2101 tgacagcaac acctatatca taggaagtga cggagtatta ctaggttata atgaacgtca
     2161 cgatgtgaac aatgaacgaa tcaaaacaat gtttgttgag aaacaatttc ctgaatctta
     2221 tactgataca gtggcacagt tgatgaaaac tgaagctaat atcccaatta ctagtgatat
     2281 tactgcattt ccggtagagt caagagagaa atatccattt ggtctgacga ctgtcgttcc
     2341 gatctttgga gctggcgaac gtttgggtac gattatctta tctcgtatgg aacaatcgtt
     2401 tgcagatgac gatctagtct tagcagaata tggagctacg gttgtcggta tgcagatcct
     2461 ttatcagaag tctcgaagca tcgaagcaga tgtacggagt gcaacggctg tccaaatggc
     2521 aatcaacaca ttatcttata gcgaattgaa ggctgtacaa gctatctttg aagcattgga
     2581 tggagatgaa ggtcgtctga ctgcttctaa tattgccgat agtatcggca tcactcgttc
     2641 tgtgatcgtc aatgctttac gaaaattgga atcagctgga atcattgaat ctcgttcgtt
     2701 aggcatgaag ggaacttatt taaaagtatt gaattcccgt tttaaagacg agttagcaaa
     2761 acacagttat tagaagtaag gggactgggg cagatagaaa tgtttgttcc agccttccca
     2821 ttttaaaaag taagtcgaag ttggaggaat ggcaatgact gttattattc aaaatgacca
     2881 gctgatcgcc gaaatcgctg aacacggtgc ggaattgatc agtctgaaat caaaagaaca
     2941 tggtttagag tatatctggc aaggtgatcc agcgtattgg ggcagacatg caccggtttt
     3001 attccctttt gttggacgtt taaaagatga tcagtatgtt tatcaaggga aaacatatca
     3061 aatggggcaa cacggatttg ctcgggatat ggattttgaa gtcattgaac atggacaaga
     3121 aatggctagt ttcatcttaa ggagtacgcc ggagacatta gaaaagtatc catttgattt
     3181 tgaattagtg atcagctatg aattaggagg agacggcatc acggttcatt atcaagtggc
     3241 aaataccggt gaaaaagaaa tgttcttttc tatcggtgga catcctgctt tcaatgtacc
     3301 tcttgaagaa ggattgtcat ttgaagattt ctatctggca ttttctccat tgaaatcaag
     3361 attacgccta ccattgaaag gcccattcat cgatatggat caaaagacgt taggtcagac
     3421 aaatacgaat ctggctttga atcgtgaact ttttgagcaa gatgccttgg tttttgaaac
     3481 aaggggtcta aatgcgtacg ccattcgaag tgaaaaatct tcccatagcg taactttaag
     3541 ctataaagat atgccttatg tagggatctg gtctccttat ccaaaagaag cgcctttcgt
     3601 ttgtatcgaa ccgtggtgcg gaatcgctga cacggtgaac agtagtggaa acttgatgga
     3661 aaaagtaggg atcaatcaac taggtgccca tgaaatcttc aagacaaaat attccatcac
     3721 agtgaagtaa gaggttgtga ccgagaagtt agcagggaac accgtttacc aaggtgtgta
     3781 aaaaagctat cctgtaagta ataagggagt gtgacatcga tttgtcacac tcccgtttta
     3841 tctctttgga atgaaaaagt atcaggattt tttcttgctg ctattcaaac caaacgggat
     3901 tcgactttca gtatggtttc tgatacgctt gatattgtct ttatgccgat aaaagataaa
     3961 ggaagaaact aaaaaggcaa tgatcgtcag taaccaattg ggcttagcca aaatcacagg
     4021 agctacataa ggaaggacga tcgtagataa agtgatcaat actgcactga tcatactgct
     4081 caaactcacc atacttgtac aaaataaact aacgataaag atgatacaag aataaacaaa
     4141 aaatacagga ttaaatccta aaagcattcc tgcacttgtc gcaacagctt ttcctccttt
     4201 gaacccagca aaaatcggaa atgtatggcc aagaatggcg cataccccaa accaaagagg
     4261 gttaatccca gaaaggtgga agatcatcgg caaacaagta gccaatgtac cttttaaaat
     4321 atccataagc aggacaacaa tcccggctgt tttgcctaat acacgaaatg tattggtcgt
     4381 tccagtatta ccgctcccgt gttgtcggat atcttttttg aaaaacaatt tgccaatcca
     4441 gacaccggaa ggaatagaac ctaaaagata tgctaaaata agtaataaga tcaatttcat
     4501 tttttaccct ctttcaagtt gaaaactatc ctttatttta gcatgaatag gtaacaggaa
     4561 caattgaaaa aatgaaagat acaagtataa tagatggaat aaaaagaaaa gcaaggggtg
     4621 gcagaaatgt ctgtaaaaaa tccaacacaa gaaacgattt tcaactattt ggaagaagca
     4681 aaaaatattg ccgtcgtagg cttgagcaat aaaccagaac gaacaagtta catggttgca
     4741 caggtattgc aagacaatgg atataaaatc ataccagtaa atccattatt ggctggtgaa
     4801 gaaattctag gcgagaaagt ctatgcgaat ttgacagaaa tcgatgatcc aatcgatatc
     4861 gtagatgtat ttagacggag tgaattttta cctgaaatag caggtgattt tttgaaaact
     4921 gatgctaaag tgttctgggc acaattaggg attgaaaaca aagaagcagc tgagatgcta
     4981 agaaaagcag gacgtacaga tatcgtaatg gatcgttgta ttaagattga acttggtaag
     5041 ttgaaagaaa accaaaaata aaaatgacca caaagaagag atgatcgata gaggggaatg
     5101 tcgaaaaagc ggttaggaga taattaaaat tgatttctcg tctaccctct tttttttcac
     5161 gaccttttct agtatgataa tcgtggtgcg gttttgccgc aaagtattat tgaaggagcg
     5221 tttgccttgg caaaaaaagt aaacaacgaa tacaatgact cgtccatcca agtattggaa
     5281 ggattagagg cagttagaaa aagaccaggg atgtacattg gatccactga cagccgaggc
     5341 ttgcatcatt tagtctatga aatcgtcgat aatgcagttg acgaagcatt atcaggttat
     5401 ggcagtgaga ttgatgtgac catccatgaa gacaatagta taactgttgc agatagcgga
     5461 cgtgggatgc ctgtaggaat gcatgcttcc ggaataccaa cagttgaagt gatttttact
     5521 gtgctgcatg ctggcggaaa gtttggtcaa ggagggtaca aaacttctgg tggactgcac
     5581 ggtgtaggag ccagtgtggt aaatgcctta tcgaaatggc taattgtaac gatcgttaga
     5641 gatggcgtag agtatcagca gaaatttaaa aacggcggta aacctgacgg tactttgaaa
     5701 aagataggaa aaacaaaaaa agcaaacggg acgactgtcc attttttacc agatgataca
     5761 atattcagta caacgaaatt ttcatatgaa attttagccg aacggttaag agaatctgcc
     5821 tttcttctaa aaggcgttaa aatctctctt tctgatcttc gtggagaaga accagtcaag
     5881 gaaatttttc attacgaaga aggaatcaaa gaatttgttg attatctgaa tgaagaaaaa
     5941 gatacattga caccagttgt ttatttttca ggagaaaaag aaggtatcga agtagaggtc
     6001 gcttatcaat acaatgacgg ttattcagaa aatgttttat cttttgtcaa taacgttcgg
     6061 acaaaagatg ggggaaccca tgaagctggt atgaaagcgg cgatgacgaa atcttataat
     6121 gaatatgcga gaaaagttgg tttgctgaaa gaacgggaca agaatctgga aggtagtgat
     6181 ttcagagaag ggttagctgc tgtactttct atccgtgtac ctgaaaatct tctgcaattt
     6241 gaagggcaaa caaaagaaaa acttggtacg ccagttgctc gtaccgtagt agataacgtc
     6301 atcagtgaac aaatgggctt ttatttgcag gaaaatagcg aaatgagcca gatgttggtc
     6361 cgtaaagcaa tcaaagccag agaagcgaga gaagcagcac gtaaagccag agaagaaagc
     6421 cgaaatggga agaagcgtaa aaaaggcgaa tctcttcttt caggaaaatt gacacccgca
     6481 caatcccgaa accctaagaa aaatgaattg tatttagtcg aaggggattc tgctggcgga
     6541 tctgctaaac aaggaagaga cagaaagttc caagcaattc tacctttacg aggaaaagtc
     6601 atcaataccg aaaaagctaa aatgcaagat atcttgaaaa atgaagaaat caatacgatg
     6661 atctatacga ttggtgcagg agtcggtcct gagttttcta tcgaagactg taattacgac
     6721 aaagtaatca tcatgaccga tgcggataca gacggcgccc atattcaagt attactgctt
     6781 actttctttt atcgttatat gaaaccgctg attgaagctg ggaaagtata tatcgcgtta
     6841 ccgcctttat ataaagtatc caaaggacaa ggaaagaagc aagtcattga atatgcgtgg
     6901 acagatgacg aattggcagc aatgattaaa aaagtcggca aaggatatat gctgcagcga
     6961 tataaaggac ttggagagat gaatgcggaa caattgtggg aaacaacgat ggatccgact
     7021 tcccgtacgt tgatccgcgt ccgtatcgat gatgctgcgc aagctgaacg tcgagtaacc
     7081 acactgatgg gtgacaaagt agaacctcga agaaaatgga tcgaaaatca cgtgcaattt
     7141 acgctcgaag aagatggcag tatcttagat aaaaaagagg atactgaaat ctctccttca
     7201 gtttctaatg atttattaga tgaagaacga gcagataaaa atgaaaacaa tcagttattt
     7261 gaagtagaat aggaggtcac ctgatggaaa atcgacaaga agtacaagaa ttaactttag
     7321 aagaagtgat gggtgaccgc ttcggacgtt attccaaata tattatccaa gaacgtgcac
     7381 tgcctgatat ccgtgatgga ttgaagccag tgcaacgccg gatattattt gccatgaaca
     7441 aagatggcaa tacgtatgac aaaggattcc gtaaatcagc aaaatctgtc ggaaatatca
     7501 tggggaatta tcatcctcat ggtgacatca gtatttatga agcgatggtc cgcatgagcc
     7561 aagactggaa gttgcgggaa gtactgatcg aaatgcacgg gaataacggg agtatggacg
     7621 gtgatccacc tgctgcgatg cgttatactg aagccagatt atccaagtta agtggagaga
     7681 tgctagcaga tatcgagaaa gaaacagtcg atctcgtgtg gaattttgat gatacagaaa
     7741 aagaaccaac agtccttcca gctcgatacc caaaccttct cgtcaatgga tcgacaggta
     7801 tatctgctgg atatgcaaca gaaatcccga ctcataactt agctgaagtg atcgatggca
     7861 caatctatat gatcgatcat ccacaagcga gccttgaaaa attgatggaa tatattcctg
     7921 gacctgactt tccaacagga gggatcttgc aaggaaaaga tgaaatcaaa aaagcttatg
     7981 aaacaggacg aggaaaagtt attcttcgtt cgaaaacaaa gatagaggca atgaagggta
     8041 ataaacagca aatcgtgatc acagaaatcc catatgaagt aaataaagcg accttggtga
     8101 aaaaaatgga tgagatccgt ttgaataaga aaatcgatgg aatagctgaa gtacgagatg
     8161 aaagtgatag aacaggtctg caaattgtcg tcgaattgaa aaaagacgcc aatgctcaag
     8221 gaattttgaa ttatttgttc aaaaacactg agctacagat caattacaat ttcaatatgg
     8281 tcgctatcga ccatatgact ccgcatcaag ttggactgaa agacatttta agaagttata
     8341 tcgagcatcg aaagcaagtc ataacaaaac gtagtcaatt cgatttagcc aaagctcaaa
     8401 aaagacagca tatcgtagaa ggattgatga aagctttatc gattctggat gaagtaatcg
     8461 caacgattcg tgaaagtaaa gacaaaaaag acgccaaaaa gaatttagtc gacgtctttc
     8521 aatttacaga agaacaagca gaagcaattg tcacattgca attataccgt ttgacgaaca
     8581 cggatatcac agagcttcaa aaggaatccg aaagcttgat tgcgcaaatc acagagttga
     8641 ataaaatcct ttcaaatgac aaagagcttt tctctgttat gaaaaaagaa ttgcgagaag
     8701 tgaaaaaaaa ttattcttcc gctcgtctaa cgacaatcga agacgaaatc gaagaaatca
     8761 aaattgatac gcaagtctta gtggcacaag aagacgtaat tgtcagtgtg acgcgagaag
     8821 gctatgtgaa gcgaacaagc cttcgttctt atagtgcttc caaaccagaa gaaatcggga
     8881 tgcgagaagg cgactacttg ctatactcag gtgaattgag tacattggac catgttcttc
     8941 tgatcacaaa taaagcaaat gtgatctacc gccctgtcca tgaactaccc gatttgaaat
     9001 ggaaagatgc tggtgagcac atttctcaaa caatctccaa tctgtctgta gacgaatcga
     9061 ttttagctgt tttcccatat caaaaaattc aagcagagaa aacatttgta ttcatcagca
     9121 agaatggatt gatcaagcaa acacgaatga cagagttcga accatggcgc acctataaga
     9181 gccgtccgct cagctgtatg aagctaaagg cggaagaaga tgaactgatt gctgtgtatc
     9241 tgaaagaaga tcagccaggc ttagatgttt tcttagtaac gcatcaagga atgggtttgc
     9301 gctatccttt aacagatgtt cccgttgttg gagcaaaggc agctggagtg aagtctatga
     9361 atttgaaaga tggagatagg attatcaacg ggctgcttgt gttagcagaa ggtgatacgc
     9421 cgatcgtaat cgttactcag cgaggagctg ttaaacgcat gcttgctcaa gaaatcagcc
     9481 aaacttcacg tgcgaagcgg ggagtgacgg tcatgcgtga gctgaaaaaa aatccgcacc
     9541 gtattatcta tatgtcggaa ggaagatcaa aagaaataac cttgatgaat caaaaagggc
     9601 agcagttaac gattgaccca actgatttcc ctataggtga tcgaacatcg aatggatcat
     9661 ttgcgatgga tgagaaaaaa ggtggagaag tcatcgaagt tatcgatgct cctgagattt
     9721 tcatcgagtc gtaactgttg tagtatttta atgaagagac gccatctgta ttaatacagg
     9781 tggcgtctct ttttcattca tttttaataa atccttatac gtttgcaaaa aaagatgaaa
     9841 gtgttcacaa tgcttaaatg ttggtatgtc aacgttgatt taaatatatg taaataaaac
     9901 ataaaaattg ttaaataaaa aacaagaaaa cccttgcaaa gatagggtgc tgtgttatac
     9961 taatcacgta aaaactgtta cataaaacag aaatttagga ggtccataca atggagcaat
    10021 ggaatggatt taaaggtacc aaatggaaag aaggcgtaga cactagagac tttattcaaa
    10081 ataactacac tgaatatcgc ggtgatgaca gctttttgga aggcccaaca gaagcaacag
    10141 aaaaattatg ggatgaattg caaaagttaa atgatatcca attcgagcgt aacggtgtct
    10201 atgatatgga cacagaaatc gtatcaacta ttacttcaca cgatgcaggc tatttggata
    10261 aagatttgga aaaaatcgtc ggtctacaga cagacaaacc tttgaaacaa gcattcatgc
    10321 cgttcggcgg aatcaatatg gctaacaatg cgctaaaaag caatggctat acaccaaacg
    10381 atgaattgac acatattttc actgactggc gcaaaacaca taatcaaggt gtatttgacg
    10441 cttatactgc tgaaatgcgt gctgctcgta aaaacaaaat cattacaggg cttccagacg
    10501 cttacggtcg cggacgtatc atcggtgact atcgtcgtgt ggcattgtat ggaatcgact
    10561 atttgatgga acaaaagaaa aaagatcatg atatgactgg ttatcataca atgagtgaag
    10621 aagtgattcg tttacgtgaa gaaatcactg aacaatatcg tgcattagcg caaatgaaag
    10681 aaatggctgc gaaatatggc tatgatattt ctaaaccagc tgcaaatgca aaagaagctg
    10741 tccaatggct atacttcgga tatttagcag caatcaaatc acaaaatggt gctgcgatgt
    10801 caatcggacg tatctcagca ttccttgata tctatatcca acgtgatttg gataatggag
    10861 taatcacaga aaaagaagca caagaattga tcgaccactt agtaatgaaa ttacgtatgg
    10921 tcaaattcgc gcgtacacct gaatataacc aattattctc aggattccct atctgggcta
    10981 ctttatccat tgcaggtatg ggattagatg gtcgttcatt agttacaaag aatgatttcc
    11041 gtctgcttca tacattaaca aatatgggac cttcaccaga accaaacttg actgttttgt
    11101 attctgaaca ccttccagaa ggattcagaa catatgctag cagaatcgct atcgaaagtt
    11161 cttcgattca attcgaaaat gataatcagc ttcgtgaaca atggggttct gatgattgtg
    11221 cgattgcttg ctgtgtatca ggaacagttg tcggaaaaga catgcaattc tttggcgctc
    11281 gtgctaactt ggctaaaaca ctattatatg ccatcaatgg cggagtcgat gaagtgactg
    11341 gcgcccaagt tggaccagaa tatcgtccaa tcacatcaga aatactagac tttgaagaag
    11401 tgaaacataa cttcatgaat atgatggatt ggttagcaga actatatgtc aatacgttga
    11461 acatcattca ttatatgcat gataaatatt cttatgaagc agctcagctg gctttgatgg
    11521 attctgactt gcaacgcaca tttgctacag gtattgctgg gatctctcat gcggcagaca
    11581 gcttgtcagc gatcaaatat gcaaaagtaa aagcaatccg taatgaaaaa ggaatagcag
    11641 ttgattttga agtggatggc gacttcccta aatacggaaa tgatgatgac cgtgcagatg
    11701 aattagtttc atggatcttg gaatacttca tgggacaaat caaacgtcat aaaacttacc
    11761 gcaactcaaa accaacaact tcattattga cgattacttc aaatgttgtt tacggtaaaa
    11821 atacaggaaa tactcctgat ggccgtgaag ctggaaaacc acttgcgcca ggagcaaatc
    11881 caagttacaa tgcggaacaa agtgggttat tagctagcct gaactcaaca gctaaaatga
    11941 attacggctg tgcattggat ggaatttcaa atacccaaac aatcaatcct tcaggtcttg
    12001 gaaaagaaca agatacaaga atcgaaaact tgcgtaatgt cttagatgga tacttctcaa
    12061 aaggagctta tcatttgaat gtcaatgtat tcagtaatga tttattgcgt gatgcaatgg
    12121 aaaatccaat gaaatatccg aacttgacga tccgggtatc tggttatgca gttaaattcc
    12181 gtgacttgac aagagaacaa caattagatg tcttgatgag aacttcacac gacagaatgt
    12241 aaaaagttcg tgaaaaaagt ttttcgactt agcgtcgaaa ggacgttgaa tagccatcgc
    12301 atcaaaatcg aataatcagt atgcttattt gaaagaaacg atttacaggg gcaggacttg
    12361 tatgttccgt ccctgtaaat tttacacata acgaagcggc aattgcgaaa aattttgttc
    12421 agaaagcttc gcttcgacag ctaacttgta cttaaaatga aatcaaagaa aagatgtgaa
    12481 cttaaatgga agaaaaaaca atcggctatg ttcattctat cgaaactttc ggttcagtag
    12541 acggacctgg actgcgcttt gtcgtgttta tgcaaggatg ccgcatgcgt tgccaatttt
    12601 gtcacaatcc agatacttgg aatattggcg gagggaaaga atacacagca gatgaacttt
    12661 tagataaagc agaacgcttc agaccttact ggggagataa aggtggtatt actgttagtg
    12721 gtggagaacc tctattgcag atcgattttt tgatcgaact atttaaaaaa gcaaaagagc
    12781 ggaagatgca tactacattg gatacatgcg gaaaaccttt tacgtatgaa gaacctttct
    12841 tttcacgttt tcaagaattg atgaagtata cagatttact gttatttgac atcaaacaca
    12901 tcgataatga agagcataaa aaactaacgc attgggacaa tgaaaatatt ttagaaatgg
    12961 ctcaatattt gtcaaaaatc aacaaaccgg tttggattcg ccatgtacta gttcctgagc
    13021 gaagtgatta tgatgaatat ttgatccgac tagacaactt tattcaaacc ttgtcaaatg
    13081 tagacagagt agagattctt ccttatcata cgatgggaaa atacaagtgg gaaacacttg
    13141 gtttgaaata tccattagaa gggatcgaac cacctacaaa agaacgtgtc gaaaatgcta
    13201 aacgattgct tcatacagaa gagtatacaa aatatttaac gcgataggtc gcatcggaaa
    13261 gaagataagt attgcttctt tccgtttttt tgctattatt aaataggaga aatcacaaag
    13321 gagcgaatga catgtcaaaa attttagttt ttggtcacca aaatccagat acagatgcaa
    13381 tcggggcagc cattgcgttt tcacacttgc aaaatgaatt aggtaaagaa acagaagcag
    13441 ttgctctagg tacacctaat gaagaaacac aatacgcatt agaccacttt cagctatctg
    13501 caccacgagt agtagagtca gctaaagcag aaaatgcaga tgtcatgctt gtggatcata
    13561 atgaattcca acaaagtatt tcagatatca gcgaagtaaa tattttagct gtagtagacc
    13621 accatcgtat tgcaaacttt gaaacagcta atccattgta ctatcgtgca gaaccagttg
    13681 gatgtacaag tacgattatt ttaaaactat ataaagaaaa ccaagtaaca attcctaaag
    13741 aaatcgcagg aatcatgctc tcagctatca tttcagatac attgttgttc aaatcaccaa
    13801 catgtacgcc tgaagatgaa gcagctgcaa aagaacttgc agcaatcgct gaagtcgatc
    13861 taaacactta tggacttgaa atgttgaaag caggaacaaa tctaagtgat aaatcagctg
    13921 cggttcttct tgatttggat gcaaaaagtt tcccaatggg cagttccaac gttcgtatcg
    13981 ctcaagtcaa tacagtggac cttaatgaag tgatggatcg acaagcagag ctagaagcag
    14041 caatggaaga tgaaatgaaa gcaaatggct acgatctgtt cgtattagtt gtcacaaaca
    14101 tcttagacag tgattcagaa ttacttgttg ctggtaaagg aaaagaaaaa gtagaagaag
    14161 ctttccaaac aactttgacc aacaaccgtg ctttgcttaa aggtgtcgtt tcacgtaaaa
    14221 aacaagtcgt acctcaattg acggctgtat ttagctaatc aaatggtagt atgaagcatt
    14281 caaaatgaat ggataagcga ttgatataaa aagaaacata gaacatacta aaggccgaag
    14341 aaatggagga gaataacagt caattatgac tcttgttctt cctccttttt acgtaaaagc
    14401 cttttaagta aaataaaacg gatagaaaag agaacatata tgaactatta ttcaaaaaat
    14461 aaggaaaact tttatcaaaa actgaccgat gatccgcttt tttcttcatt gaccgattat
    14521 ctatatgaac atcgagaaca agaaacgatt ctaagagaat tgaaaaaaga attttcgcaa
    14581 aataaatttt cccattttct tgatttattg atcgatgcag gattaatcaa gagggaagaa
    14641 cgaagatatc atttgaactt tccaatattt gatcctaaag attacttgca acaagccact
    14701 tcggctgctg aaaccatagc tgaacaattg aagagactct ctgttgatga acaaaaatta
    14761 gcgatgggag aagttatttg ggcgtattgt ttcgaagatg aacggaaaga agcgtatttt
    14821 tatggagtaa gaaacagccg agagacagaa ttgctgagga caactgcagg aaatgagaag
    14881 taccgtttta tcacactttc ctctatagaa cacttcccgc ttactttggc taattacttt
    14941 tttgtacaga agaatcagct tcctgtaacg aaggcattca aagaattagc cgaacttatt
    15001 ggagacgtaa atgaatcgta cttttttgat caaatagaag ttatcgttga caggatacgt
    15061 aagaataaat ataaaaatcg ccggcctagt attttccatc agtctctact tgtgacgaat
    15121 actataaagg aagaagaatc attcacatta gaattaccta ttgtcgaaaa aaacaatttt
    15181 gagattgaat taccaacatt ggatccttca ttgacaatgg aggaaatggc ctttcttaaa
    15241 cgacaaatct tttctgagct gagcaagaaa tttataccgc atgcattttc ttatatcaaa
    15301 gaatatggaa cagttttagt gagtaaaacc taagagagca gtaagcaaaa attatactga
    15361 ctcttgcagg aggagccttt ctcttccttt atggttaaaa gaattatttg agtattttgc
    15421 ctatctacca cctattagat tcactacatg gtctggaaac aaaggaatgc aaaaaaatgg
    15481 tgagaaatgt ggaatgacaa aggaagtagt gataagaaaa gttagatttt taaataatca
    15541 atattatgat tcagtcaagt atggcatttt atgggaagaa ctagctgatt agattaggaa
    15601 gtccatttga taatccagaa tcactaagaa acgaatggcg agtcattaat aatttatcca
    15661 atcttgggtt attcttttag attgaagtgt ttaaaactaa aaaagcattt atgaagtcgt
    15721 aatagtcagg cttcaaaatg ctttttattt tgtttgtttt ttctttgcca cataaataca
    15781 cctagactga cgatgaagag catatccaac agatttcctg cgacttggat cgtcgaaggg
    15841 ttggtcagtg gcattaaagc aaggacaaaa aaggaaaaca agctgtaaat aaaacaggtg
    15901 acaggaacta atcgaaagaa gagcggacat ttcaattggc taaaaatgag tgaaaaaata
    15961 aaataagtaa aaatggataa aatcacgact aaaccattca tgataaagaa gaaaagcgta
    16021 ttgtgattaa acgaataaaa aagaattgct aaaaaagaaa acgttttaaa tgcaatcaat
    16081 acgcttgaag cttcgtttaa cgtaccagga tgtcgatgaa aaaaataggc accgaaataa
    16141 acagaaaaca gtaagccgca aagagaagac aaaatcaaaa aaaacggatt ttcttcggct
    16201 tctgtgatat cagaatgatc caaaagcaac cagcctaatg cactagaagg cgcatgtgag
    16261 gtcttaaggt atgaataaca tagaaaaaat aagatcgtca aagttgcaaa aaaagagcct
    16321 aaatgtaaaa aattagtggt tctacgttga atttccataa tatctccaaa ttaaattgat
    16381 aagtatataa agtataataa caaattgatg agtatatgtc gatgtttttt aatcaaaata
    16441 ataaataaaa actgtctaac atttaaaatt taatttagat attagacagt ttttaagttt
    16501 gtgagttatt ttccttgtcg ttttctcact tttttagtcc agaaaccacc tgaaatatat
    16561 tttggctcat aagaaatgat aaaagcacgc tcttcgattt ctttgatcag gcgatacagt
    16621 tcacgttcgc tttttctagg agaaagtatt tcgagaacta gccgctcacc ttccagcccc
    16681 attgcagaag tctgtgtcac accataaccg tgttctctca gtatacccgg caaactttta
    16741 tcttctgcgc tatttgctgg caaaatcgtt gtgaccatga tgtatcctaa tgccaatttg
    16801 tcttcaatca aaattccgac actgatacct actgcgtaac ctaacgcata gacaaataaa
    16861 ttcagtggat tatctaaacg atctaaaaca agggataagc cgacgatata aatcgtgatc
    16921 tcgaccatac tgacaagtgg agcaatcagt cgatagcctt tcattgtcaa catgaaacgt
    16981 aacgtgttca gagtgatata agcaaaatta atgaggaaga tcatccccaa catttttgga
    17041 tcaatcattt gtaaaactcc tttcatttat ataacaacaa tatcaaaaaa aatcgtaagt
    17101 gaagaaaaat tgaaacttta tacatacaaa aaaatgataa aatgattata atagaattat
    17161 aaagataggt taggtgatta aaatgaaaac ggtcaatcgt gtgttttgtg ccagccacgt
    17221 gctaagtctc cttctcagta tatacttagg tgtctattcg attcaacagc caattgaggt
    17281 agagaacatt gtttttcaga tgtcgctgac agatggttta attttaagcg tttttttctt
    17341 tttgatactg tttattggta atattttcgg tagcgttgcc agttttttga atttttctat
    17401 ttatccactc ctttcgctag cattaggagt ggtgggactt tgttctctgt gcttatttcc
    17461 cgagccattc agtcctgcat ttatattatt tggtacactg aacctgttcc agacaactgt
    17521 tggagcatgg ttgctatggc gatcaggaaa tttaatgaaa ataggtgagt aacaattgaa
    17581 tccaattata ttgaaacgtt ctgtccatca tattccagtg ttagaagttg ttctgaaaga
    17641 attaagagag gaacgtattc ccgttgtcat ctattatcac ggatggcaga caagtaagga
    17701 gttagttttg acgcaaggaa gaaaaattgc gcagcagggc attcgtgtga tcctgccaga
    17761 tgcgatgaat catggggagc gcaaacagcc ggtttcttct attccatcct atacattttg
    17821 ggatagtatc tacggaaatc tgtttgaatt tgacacactt atcaagcatt tagaaaaaag
    17881 aggtcttcta catgatcagc ttgcagttgg cggggtttcg atggggggaa tgacaacatg
    17941 cgccttgttg gctaaacatc ctcatatagt agcgggcgtg tgtttgatgg gttcccctgc
    18001 acctttggaa tatggaaaag aaatcgtaag gcgcgcatcg gaatataatt atgtcgtacc
    18061 aaaagactat tttgatttga tttcatgggt taagagctat gatttgtcat tgcagccgga
    18121 aaagttagct ggacgtcctc tgttcttttg gcatggacaa gctgacgaaa aaatccctta
    18181 tatacaaacg gaacgatttg tagaaaaaaa tcggcataag tcttacggaa aagaaattgt
    18241 ttttcgttcc tcaaaaacac agcgacatat ggtacaaata ccactaatgg aagaagcttc
    18301 cactttttta gcaaaaaatt tgataaaaaa gtataaataa ttgaaacgga aaatcactca
    18361 tgacgataga ggattttccg ttttttattt tgggctataa taaactctgt taggaaaatt
    18421 tggtataatt gatagtaaga atgacaacat gttagaaagg aagagagcat gcccacttta
    18481 gcagatgttg caaaacgtgc caatgtttca aaaatgacgg tctctcgagt catcaatcat
    18541 ccggaacagg tgactgacga attgaaagag ttagttttct cagcaatggc tgaactggat
    18601 taccgtccaa atattgctgc aaaagcactt gtcacgaatc ggtcgcagat catcaaatta
    18661 tttatactgg aagaaatcga cacaactgag ccctattaca tgaatttgct gatgggaatc
    18721 gctaaacgga tcggtaaagc gcattattct ctccaattag ttacaaatga tgccttcgac
    18781 gtcggttctt gtgatggata catcatcaca ggtgtcagaa aaaaagactt cgattggatc
    18841 gaccgtttgg aaaaaccagt tgtcttgttt ggggaaaatt ctgaaggata tgattatgta
    18901 gacagcgata atgaatatgg tacagcaaaa gcaacgcaat atgccttaga aagagattat
    18961 caaacgatta tttatatcgg tatcgaagtg gacgagcctt ttgaaatttc aagggaaact
    19021 ggttatttat ctgtgatgaa ggaagttgat cttcctagta aaatcttgcg gtttccaaat
    19081 cattcgacag aagtagaaag atatatgaca gaaaactggg cagattttac agataaaact
    19141 tgttttgtat gtagttcaga tcggctagca ctaggagcag taagagggat tataaatgct
    19201 ggaggacact tgcccgaaga atttggtgtg attggatttg atggggtatt tttagaccaa
    19261 atcagttcac ctaaattgac aacgatcaag caagatatta tacgtatggg tgaagtatgt
    19321 ggagaaatgt tgttaaaaaa aatagaagaa aaaggggcga gtcaaggtta tcgtcgtttt
    19381 tttcctgaac tagttatcag agagacaacg aattaagtct tattactata tatatattta
    19441 tgaaattcat cgctattttc ttaaaataat cagcgcattg ctactgaaaa caatgaatct
    19501 gtgctattat tgagaaaagt tccatttttg agaaggatgg tttgaatgaa agaaatcaga
    19561 cgaaaacatt tccgttttcc tgcgtacgat gatgagttag gcgtaaaatt aaccaacgac
    19621 aagaagcgag tattatttga cggacaagaa gatcttttga ctgaacagaa tcagtccccg
    19681 tttgaacaga tgaaagaatt tcatttcacg aacacgaaca ccgagaaaat ccgtcgcaag
    19741 cgtcgtgcaa caaatggatt gaagtcagct ccgcgtaagg aaaatttgga aaagcataaa
    19801 ggaaaattac cggattatac cggccattac caatcagaaa tcacatcgac aggacgaaaa
    19861 aaactttttg gtgattcctc tcgttctgtc agacaaagtg aaacaaggac agagtccaag
    19921 catactacac gaaacgcatc tgttgacacg aaggtgcggg agcgttctta ttttgttcct
    19981 aaatatgtac ccgcatcgat tatcccagac cctagagaaa gcaaaatttc tgaggatcat
    20041 ttaatcgaat cgatgaaaaa aacggatgac tcttatctgt tgttcgatac agaaccgacg
    20101 ccgtatcaag aaaaaagaaa tggtgagccg tctgtacgaa cattcaatcg aacaaaaacg
    20161 ttagaggaaa caaagcagtc agaagaaaaa aaatcgaaaa aaagccatgg tatcttagaa
    20221 cgctcattac aaggattgat cgatgatcaa ggaaactctt tgggagaaaa tagctatttt
    20281 aaataagtga tatacaaatt tatattcggc tacagttgga aaggaaacgc tgcggcgttt
    20341 ccttatttta tactctatcc gaaagtttat atcgtatcct caaatttttt tgagtttaag
    20401 cgaatttttt ctcccaataa gtaagaaaat aatggcaaaa taaaaaaaca aactattttc
    20461 gcagatcgtt tgcaaaaata gtttattttt ttataaagaa gattttactg aataatatca
    20521 ttttagttta cataatgata tttctgtaaa ttaaataaag tagtgaaaaa taagaatcag
    20581 tgctctaact gttcaagagg atcaagtggt tcaaatgttt ctgtagtctg ttttgcttcc
    20641 aattggggca aaacagatcc aatttccaga tacttcactt tattcaatgt ataagataga
    20701 atagaacagc taagtaatgt atacagaaaa tgtgtgagat ccaaataaag caaggagaca
    20761 actaaaatac caaaagtaat caagaaaaga cttttgccaa tcttaatatg aaaatatttt
    20821 tcaatggctt gataaatgat atctgttccg cctgtgcttc cgccgaatcg aaagacgaga
    20881 ccgcaaccaa tgccagaaag aatacctgtc atcaaactat tgatcagtag actatctaca
    20941 gcaaaatgta tatgaaggct ctcccataaa tgtaaaaaga aaactagcgc aaatgttccg
    21001 acgatcgtcc ggagaaaagc gtgttttcca aaaagaaaca atgagataag caataaaggt
    21061 aaattaaaaa gcagtgaagt gacagaagga gcgaatccaa aaagattatg caaaagcaaa
    21121 gcaataccag taaccccacc atctgccagc ttagatggaa tattgattcc tgtcacagct
    21181 aatccgtaga ttcctgcgcc taaaatgatt ccgcacaatt ctgtaaataa atttaatttt
    21241 ttcatttcta gcctcttttc ttttgaaaat tttcagcaac ttctttatta tactgactta
    21301 tactcttact tcaagaggaa aatgaaaaaa aattattttt ggctgtttct ttttatttat
    21361 caaataagga gtggaaaaat gcgagcagca gacttattga caattcttac aaaatcaccc
    21421 aaaaaaacag agtatcaagg agtttttttg gctcaagaac aaaaactggt gcctgtgaca
    21481 atggtccaga taaataaagc aaatcaattt atctttttat ttgaacacgg caaagcacca
    21541 cttgctatga aagacatctt tctttttttg atgaagaacc gtgagaaagc tgtttattat
    21601 cagtcaaaag aagaactcat tcctctttat ggagtgaaag agatagaaaa taaagtggtg
    21661 atataagtaa aaataagaaa acaagacgtt tatgatgtca aaacaaccat tttttacatt
    21721 tttggcaaaa aagtgtcatg atacagtata atattcgttg aacaggccaa taaaggtgtt
    21781 ctcccccacg agatttctac tttattggct gtttcttttt attaaaataa aagttgtgtg
    21841 tgtaaaaaag atatagctga ttccaagtga caatttcgta tagattttta tggtattcgt
    21901 agtgaaaaaa cgagaaccaa aaagttgatc cgactttgtt gcttggattc tttttttgtc
    21961 agcatattta gaggtatttt tttctttttt ttgttcgtgc tgtaaggtta aggcaagaca
    22021 agttagttgg gaaaaaggaa agaaatgttt atacttaaag aataacagaa aggaaggtcg
    22081 catgaataat tttatacaaa cattaagtga aagaaaaggc gagttgctgg aagctgcatt
    22141 tcaacatctg tccatttctc tcatttcttt gctgattgca gcactgattg ctattccttt
    22201 agcgatttgg gcagccaatc ataaaaaaat ggcagaagtc ttattgcaga tcacgggtgt
    22261 gctccaaacg attccttcac ttgccttatt aggtttactt atcccatttg tagggatcgg
    22321 aactgttcca gcattgattg cacttgttat ttacgctttg cttccgattt tccaaaacac
    22381 gtatattggt ctagctgaaa ttgacccgtc tattgaagaa gcagcggtag catttggtat
    22441 gtccagaatg agacgcttgc tgaaagtaga gcttcccatc gcattacctg tcattatctc
    22501 aggtatccga actgccttag ttttaatcat cggtacggct actttggcag cattgatcgg
    22561 agcaggtgga ttagggacat ttattttgtt aggtattgac cgaaatacac ctgttttgac
    22621 attgattggt gcgatcagtt ctgccttgct tgcaattata ttcagcggac tgattcgctg
    22681 gttgcagcat aagaaacctc gatattccat cattacttta gcagcagtgg tgtgtttatt
    22741 tggcggcgtt tctttatatc agtctgcctt gtttaaaaat gaaaccatca cgattgctgg
    22801 aaaattagga gcggaacctg aaatcctgat ccaaatgtat aaagaattga ttgaagacaa
    22861 cacaaaaaca gaagttgaat taaaaccgaa ttttggcaag acgagttttt tgttcaatgc
    22921 actggatcaa ggtcaaatcg atatctatcc tgagttcaca ggtactgtcc tagaaagctt
    22981 ggtgaaggta cctgaagcaa tgaaaagtgc tgatttgaca aaagaaaaaa catatgacga
    23041 agcagaagaa ttattgaata agcaattcga tatgacatta ttaaaaccaa tggcttacca
    23101 aaatacgtat gccttggcag tcaagagatc ttttgcgaac gaacatcaac ttaaaaagat
    23161 ttccgatttg gcagctatct ctgatcagat caaagcggga ttcacactgg aatttattga
    23221 tcgttcagat ggatataaag gaatccaaga agcatatggc ttgaattttt caagtgtaca
    23281 aagcatggag ccaagtttgc gttaccaagc aatcaataat ggagatgtca atattgtgga
    23341 tgcctattcc acagatagcc agctcaagga gtacgatcta gtgactctag aagatgatcg
    23401 agggttattc ccagcttatc aaggcgcacc gctgatgaaa aaagattttg ctaaagaaca
    23461 tcctgaaatc gttaaagcac tgaataaatt aagcggaaaa ataacagagg atcaaatgat
    23521 tgatatgaat taccaagtca atgtcgaaaa gaaaaagccg acagaagtag cacgaatctt
    23581 cctgaaaaat gaaggattag taaaggagga agatcagtga ctaaattaat cgagttcaaa
    23641 catgtgcaaa aaaagtatga tggaaagtat gtcatcgatg acctaaatct ggcaatcaaa
    23701 aaaggagaaa tctttgtttt ggttggtcct tccggtagtg gaaaaacaac tactttgaaa
    23761 atgatcaatg gattatctaa accatcagca ggagacatct acttcaaagg aaaatcgtta
    23821 aatgaatata atctgcagaa aatgcggtgg aatatgggat atgtgctgca gcaaatcgct
    23881 ttgttcccga ccatgacagt caagcaaaat attgaagtca tccctgaaat gttaggttgg
    23941 gaaaagcaga aaagagcaga ccgtgtagat gaacttcttc aaaaagttgg cttatctcct
    24001 gatatttatc gagatcgtat gcctagagag ttgtctggag gagaacagca acggatcggg
    24061 atcatccgag ctattgctgc aagcccagat gtgattttga tggacgagcc atttagcgca
    24121 ttagatccga tttcaagaaa ctcattacaa gaattggttt tgtccttaca cgaagaattg
    24181 ggcacgacca tcgttttcgt tacgcacaat atggaagaag caatcaagct aggtgatcgt
    24241 attgcattta tgaaggatgg agaaattatc caatgtgaca caccagagca gttattgatg
    24301 aatcctaaaa acgactacgt tcggcatttc tttgatgaac cgaaacagac gaaagagtgg
    24361 cgagtagaag atttagtcgt caatggatat ttcttaaatg aaataccaga aaatgctcgt
    24421 caacaagttt gctttgatac gcctatgaaa gaagtatatt cgcttctttc ggtgtatcca
    24481 agcattgtga tagtagaaaa gcaaagaagt attgggtcgc tgacaagtaa agaaatcttt
    24541 gcttttttaa gtcgagagga ggaaggaaat gaaaacatat gatgcaatca tcatcggttc
    24601 aggtgtgagc ggcttaagtg ctgcttatgg attgaaagaa gcaggtaaaa cagtactagt
    24661 ggtcgaagaa gatctatggg gcgggacttg tcctaaccgt ggatgtgatc ctaaaaaagt
    24721 cttgttaagt gcagtagagg cacgtaatcg ggtaaaacaa ttgagtggaa aaggttttaa
    24781 cgagatccca actgccaact gggaagaact tcaaaaattc aaacgaacat ttacggatcc
    24841 agtcccagaa agccgaaaaa aacaattagc agaagctgaa atcgatcatc tttcaggaac
    24901 cgctcgtttt ttggatgatt catctattga agtcaacgaa gaagtcttcc atgcggatta
    24961 cttagttttg gcaaccgggc aaagacctac gatcttacca gtggagggaa aggaatactt
    25021 gaaaactagt gcagactttt tatcattgcc tgtactgcct aaagaaatca tttttatcgg
    25081 tggtggttat attgcttttg agctagctac gattgccaat gctgcaggga gcaaagtaac
    25141 cattgtccat cataatcaac ggccattaaa agaatttgag gcgtctttgg tagaggaagc
    25201 cgtccatcaa atggaagcat ctggcattca gtttgctttt ggtgttgaaa cccaaaagat
    25261 catttcagaa ggtactcgct atcgtttagt cggaaaagag acggaattag tagcagatat
    25321 gattttttgt gcgactggga gacagccgaa tacagaatct ttagcacttg agcaagcgaa
    25381 tatcgtcttt gataaacacg gaattgcagt caatgattat ctgcaaacga gcaatccaaa
    25441 aatttttgcg tgtggagata ttgtttcaag gaaaacccca aaattgacac cagtcgccac
    25501 ttttgaagga aattatgtag ctaagcggat aacggatgca acaagcgaac ctatcaaata
    25561 tccgattata cctacgatcg tctacgccag tcctaaactt gctgaagtag gagttacaaa
    25621 aagtcacgcc tcttcgtcag atcaagtggt agagatggat cttaccagtt ggttcaccta
    25681 tcatcgtgtc aatgaaccag ttgctaaggc tgaattgacc tttgaccagc aaaattattt
    25741 gattggtgct gcagtgatta gcgagcaagc agacgaattg atcgatgatt tgacactcgt
    25801 catcaatcaa aaattgacta aaaaagagtt ggatagctat atcatggggt atccgacatt
    25861 ggcaagtgat ctctcctatt tattgaaata atcagcatga taactaaagc gcttgttgct
    25921 ttttccttct tgtgctacac tttaaaaaat gaatacctgt ttctggggat gtcgtagacg
    25981 acatgagagt aagttgtgaa cttagaccca ttgaacctgt cagttaatac tggtgaaggg
    26041 agaaacatag cagacgcttg aagcgggtta ctatgtcgtg cccatttcaa gcgttttttg
    26101 ttattcatat tagaagaaag gaggtaacaa aatgaagaaa aatctttatc cagcagtcag
    26161 tatggcattc attttaatta tttggcagat tgttgtcata gtttggaaaa tacctgcctt
    26221 tattttaccc ggacctgctg ctgtattttc tgcattagcg gcagatgcac aagtattact
    26281 ttcccattct gtcacgacat taaatgaagc agtgcttggt ttggtaatag ctagcgttct
    26341 ttcttttatt actgccttgg caatggatta ttggaagttt ttagaaatgt ctttttatcc
    26401 gttactagtg atttcacaaa tgttgccaat catggtgtta gggccattac ttaccctatg
    26461 gtttggtttc ggtatgcttc caaaagtgat ccttgttgtc ttgataagct attttccgat
    26521 cgtagtttct ttttcggata cattgaggaa agtatcaaaa gagcaaatca tctttttgaa
    26581 aacgatgggg gcagatacag ggaagattta tcgaatatat aaaataccag cgggtttatc
    26641 cggcttcttt tccggtttaa aagtggctgc tacttattgt gtcagcggag cagtcgttgg
    26701 agaatggcta agcgctcaag caggcttagg gtattacatg atacgcgtaa aaaacagcta
    26761 ccagatcgat aaagtttttg ctgcgatcat ttgtgtcatt ctattgagtt tattgctgaa
    26821 tggcgcttgt tcattattga aaaaagggta ttatcgtatg ctttatattt tttaagagaa
    26881 ggggatcaaa tgaaaaaatt atggctttta ttaccgctac tgttgctttt atccgcttgt
    26941 ggcactgcaa aagaaacaag tagcaaacaa gaaataaagg atttgaaaaa agtgacactt
    27001 gttttggatt atgtaccgaa tactaatcat actggcatct acctagcaaa agaaaaaggc
    27061 tattataaag aggctggttt aaatgtccaa atcatcgaac caggagataa ttcgacaagt
    27121 attggtctag taggtgcgga caaagcacaa tttggtgtaa gttatcaaga agatgtcact
    27181 tatgctcacg cagatggaca gaatatacca gtcaaagcaa ttgctacagt gatcaaacat
    27241 aatacttctg gattcgccac cctatcagac agtaacatcc attcaccaaa agattttgaa
    27301 ggaaaaacat atgcaggttg gcaaagtcca agtgaagaag ctgtcttaaa agctgtgatg
    27361 gaaaaagacg gcggggactt ttcaaaactg acaatggtag gaagcaatgg agaagggcca
    27421 gaaagcttag gaaaatcctc agatatccaa tggtactttg aaggttggga tatgatcaaa
    27481 gcaaaagaag cgggtattga actaaactat atcccattga aagaattaga tgaacggtta
    27541 gattactata ctccggtaat tattacgaat gatcaactga tcaaaagtga tccagaattg
    27601 gtacagtctt ttatggatgc cacgaaaaaa ggctatcagg aagctattaa ggatccaaat
    27661 gacagtgcta aactgttgca aaaatatgca aaagaaaatg atcggacgtt tttagaagaa
    27721 tcacaagcat tcttgtcaaa aaattatacg gatgatcctg agaattgggg attgatggaa
    27781 gaaaaagttt ggcgcaatta tacagctttt atgcaggaaa acggattgat caagcaagat
    27841 gtaccttcgc aagaactatt cactaatcag tttataaaat aggagtgata gatatgtcaa
    27901 tccaaatcag ccacttgaat gtagtcattg aaaacctgaa agtattagaa gacatccatt
    27961 tcactatccc taaagatacc tttacatcga ttgtcgcgcc aagcggagcc ggtaagtcaa
    28021 cattgctgaa aactttaaca ggagtccagc ctatgacttc tggaacgatc aaagtagacg
    28081 atcaaaaagt aactggtctc catacagcat ttagttatat gccgcaagaa gatatgttac
    28141 ttccttggct gagcgtctat caaaacgtta cgttatatca aaaaatcaat catctaacaa
    28201 tcaatgaaga acaagtaagg gattatctgg atatctttgg gttaaacggc tatgaacatt
    28261 ttttgccgga acaattatct ggcgggatga agcagcgaac ggccttgtta cgaacaatca
    28321 tgaatccttc ttcttacttg cttcttgatg aaccatttgg agcgttagat gctatgacca
    28381 gagggcagat gcaagactgg atgttgaagc tgcctaaaaa agcaaagcgg actactttac
    28441 ttgtgacgca tgatatcgaa gaagcaattt atttatcaga caggattctt gtgctttccg
    28501 cccgtccggc acatgtgatt gctgagattc aagtgcctga aaaacgacgg tcacgtgaat
    28561 ggctactgca gcaatcagag ttgaagcaaa cgatttatca gttattggca ggtgaattag
    28621 atgttaagtg atgctcattg tcatttagat gggagtcaga gtttagcact ccttcagcag
    28681 gaacacacga ttttgacgat catcaattgt gattcgccag aagaatggaa ggaaaacaga
    28741 cagcttgcag caagtaagac tcaagcctta agttacggta tccatccttg gaaagcagat
    28801 agctatacgt ttgaacaagt tgagcctttt ttgaaaaaag caaggatcat cggtgaaata
    28861 gggttagata atatttggac gaatgtaccg atgactacac aaaaaaaagt atttgagcga
    28921 cagcttgctt ttgcagccat aaatgagaaa cctgttgtac tgcacacaaa aggatgcgaa
    28981 aaagagatct taaattatat ccaagcttac cctaatcact atttgatcca ttggtattct
    29041 tctttggact atcaacaaga ctatattgat ttaggctgtt atttcagcat tggtcttgat
    29101 ttgaaaaaga atccagcagt ctggcaacta gctaaagcag tcccaatcga ccacttactt
    29161 attgaaacgg atggactaca tgcagcagag tgggctttag gggggatttt ttcagaaaaa
    29221 gattatgtct cactgcttga agaacagtta aagacaatcg ctgagatcaa agagctatca
    29281 gttgaagaag ttaaaaaaaa tacttatcaa aacttggaac gtttcattag attaggtaaa
    29341 taaaaaagag atgtgacaca actagtgtcg cacctctttt tggctttctt tgtataaaag
    29401 taaactgttt gattaataaa tgaatcttag taccaaccgt ttgcttccca gaatgctttc
    29461 gccgcttccc aagaaccata acgtgaagta acatattgtt ctgctactct ttcttggttt
    29521 gcagcagagt agtcaccatt taagtaagaa gaatctaatt ggtaacgtcc gatgtaacgt
    29581 ccgttcgttg ctgtgtaaga accgcttgat tctttttgtg cgatccattc ttttgcagag
    29641 cttgtgcttg ctggagtagc agcagtttct gttacagcag gagcaggttg tgtttcagca
    29701 acaggcgctg cttgtttttc cgtttgaacc gtttcttgta caacaggttg ttcagcttgg
    29761 actggtgctt cttgaacggc tgcttgaggt tcagcagctg cttgtgcaga gccttctgtt
    29821 gggattgtta attgttgtcc tgagta
//
LOCUS       DACDYI010000038        28030 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.038, whole genome shotgun sequence.
ACCESSION   DACDYI010000038 DACDYI010000000
VERSION     DACDYI010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.038"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            185..1183
                     /locus_tag="IWM52_001546"
     CDS             185..1183
                     /locus_tag="IWM52_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9973802.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1295..1804
                     /locus_tag="IWM52_001547"
     CDS             1295..1804
                     /locus_tag="IWM52_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAP9973803.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1834..2493)
                     /locus_tag="IWM52_001548"
     CDS             complement(1834..2493)
                     /locus_tag="IWM52_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAP9973804.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2495..3259)
                     /locus_tag="IWM52_001549"
     CDS             complement(2495..3259)
                     /locus_tag="IWM52_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAP9973805.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3267..3944)
                     /locus_tag="IWM52_001550"
     CDS             complement(3267..3944)
                     /locus_tag="IWM52_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAP9973806.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(3981..5366)
                     /locus_tag="IWM52_001551"
     CDS             complement(3981..5366)
                     /locus_tag="IWM52_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAP9973807.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5576..5660)
                     /gene="ffs"
                     /locus_tag="IWM52_001552"
     ncRNA           complement(5576..5660)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IWM52_001552"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="IWM52_001553"
     CDS             complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="IWM52_001553"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAP9973808.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6305..7162
                     /locus_tag="IWM52_001554"
     CDS             6305..7162
                     /locus_tag="IWM52_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAP9973809.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(7243..7578)
                     /locus_tag="IWM52_001555"
     CDS             complement(7243..7578)
                     /locus_tag="IWM52_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973810.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(7645..9015)
                     /locus_tag="IWM52_001556"
     CDS             complement(7645..9015)
                     /locus_tag="IWM52_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAP9973811.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(9093..9629)
                     /locus_tag="IWM52_001557"
     CDS             complement(9093..9629)
                     /locus_tag="IWM52_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9973812.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            9938..11419
                     /locus_tag="IWM52_001558"
     CDS             9938..11419
                     /locus_tag="IWM52_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9973813.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(11522..12229)
                     /locus_tag="IWM52_001559"
     CDS             complement(11522..12229)
                     /locus_tag="IWM52_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAP9973814.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(12308..12646)
                     /locus_tag="IWM52_001560"
     CDS             complement(12308..12646)
                     /locus_tag="IWM52_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAP9973815.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(12757..13719)
                     /locus_tag="IWM52_001561"
     CDS             complement(12757..13719)
                     /locus_tag="IWM52_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAP9973816.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(13734..15065)
                     /locus_tag="IWM52_001562"
     CDS             complement(13734..15065)
                     /locus_tag="IWM52_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9973817.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(15073..15753)
                     /locus_tag="IWM52_001563"
     CDS             complement(15073..15753)
                     /locus_tag="IWM52_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP9973818.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLIDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(15972..16451)
                     /locus_tag="IWM52_001564"
     CDS             complement(15972..16451)
                     /locus_tag="IWM52_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAP9973819.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(16578..18257)
                     /locus_tag="IWM52_001565"
     CDS             complement(16578..18257)
                     /locus_tag="IWM52_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP9973820.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            18805..20307
                     /locus_tag="IWM52_001566"
     CDS             18805..20307
                     /locus_tag="IWM52_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9973821.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            20320..20982
                     /locus_tag="IWM52_001567"
     CDS             20320..20982
                     /locus_tag="IWM52_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9973822.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(21083..21286)
                     /locus_tag="IWM52_001568"
     CDS             complement(21083..21286)
                     /locus_tag="IWM52_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP9973823.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(21397..22581)
                     /gene="iadA"
                     /locus_tag="IWM52_001569"
     CDS             complement(21397..22581)
                     /gene="iadA"
                     /locus_tag="IWM52_001569"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP9973824.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(22738..24477)
                     /gene="yfcC"
                     /locus_tag="IWM52_001570"
     CDS             complement(22738..24477)
                     /gene="yfcC"
                     /locus_tag="IWM52_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP9973825.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            complement(25250..25864)
                     /locus_tag="IWM52_001571"
     CDS             complement(25250..25864)
                     /locus_tag="IWM52_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9973826.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(26470..26847)
                     /locus_tag="IWM52_001572"
     CDS             complement(26470..26847)
                     /locus_tag="IWM52_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973827.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(27147..28028)
                     /gene="galU"
                     /locus_tag="IWM52_001573"
     CDS             complement(27147..28028)
                     /gene="galU"
                     /locus_tag="IWM52_001573"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP9973828.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT         8326 a         5874 c         4685 g         9145 t
ORIGIN      
        1 atttgcatac ttcataataa taataacata atacctaacc tctcgtaaaa atactagatt
       61 tgttttcttt tatttttcat cactagatac attctataac ttaccgtttc gatttcgaat
      121 tcaaacattt ttgagaagca ttctcaatta aattctgcta tagtgtgaaa agggaggaat
      181 ttttatggaa atctatgaca ttacaatcat cggtgcaggt cctgtaggga tgtttgccgc
      241 tttttacgca ggaatgcgcc aagctaaaac aaaaatcatt gacagtctac ctcaacttgg
      301 tggtcaatta gcaactttgt atcccgaaaa atatatttac gatattcctg ggtatccagc
      361 aataaaagca agtgaattaa tcgatcaatt agaaaaacaa ttaactactt ttaaccatac
      421 attccattta aaagaagaag ttctctcgct tactcgtgaa gacgaagtta tcgagattac
      481 tacaaataaa ggcatccatt attccaaagc agtcatttta gctttaggaa atggttcatt
      541 ccagccaaga aaattgaatt tagataatgc tgaatcattt gaaaaccacg gattggatta
      601 tttcgtgaat gatttgatga agtatgctgg aaaaaaagtc gctattgctg gaggtggtga
      661 ttctgctatc gattggacgt tgatgttaga gcctattgca agcgaagttt acttgattca
      721 tcgtcgcccg gaatttcgtg cgcacgaaca tagtgtatca cgcttgaaat cttcatctgt
      781 caacttgctt actccctatc tcatcgatgg attatcaggc aataatgggg aactgacaga
      841 tatccggtta aaaaaagtga aaagtgacga gacgattgat ttgatgattg attctctgat
      901 cgtcaattat ggattctctt ctaatttaga acatctttct tcttggggac ttgatagtac
      961 cagaaatgcc attactgtaa aatcagatat gtctacttct attccaggcg tttatgcagc
     1021 tggtgacatc tgtacctatg aaggaaaagt gaaactgatt gctactgggc tcggtgaagc
     1081 acctactgca gtgaacaacg ctttacacta tattaatcca aaggaacgaa ctcagcctgg
     1141 tcacagcaca agcttatacg ataaaaatct tcgtcctagt tgatttttta tatctctttg
     1201 catttcaagt taaacccgaa agcagtttct tgcatttttt tcatgttccg ttaacatgaa
     1261 atagagaaag aaaaaataaa gggggagcaa taatatggtc gtcaaaactg agactttaga
     1321 agaaaaggca agagaactat tgaaggaacg tggcgtaact gtagaagata tcgcacaact
     1381 tgtcatgttt ttacaaaaag actatatttc tgatttgact ttggcggatt gtactgaaag
     1441 tgtcaacagc gtgctaacaa agcgtgaagt acataacgcc attatcactg gaatccaatt
     1501 agatatatta gcagaaaaga aaaaattgat gagtccgttg caacatatta tcgaagatga
     1561 cgaaggcctt tacggtatcg atgagatcct agccttgtca atcgtgaatg tttacggtac
     1621 gatcggcttt acgaattatg gttatatcga caaggtgaaa cctggtattt taaaagaact
     1681 aaacagccac gatggcgttc atgtccatac cttcttagat gatatcgtcg gagcgatcgc
     1741 cgctgctgct gccagtcgat tagcacacaa agaaccagaa aaaagaagtc gccttactca
     1801 ataattcaac aaaaaaagaa agaaaacgtc tatttagacg agttctttct tttttgtttg
     1861 tttcaagcta ttttttccta agaaaagaaa aacagcaaaa aataattcta ataatacaaa
     1921 aaataagata aagctatgca tgaaaaattc ttctggaagt acattgatga ttggatcagt
     1981 aacgggatcg aataaccagt catcgttgtt gaaaaacgtt tcatgaaaaa ggataaaaaa
     2041 gcgatcaaat cctatcatca tgaaaaaacc gaaaacaact ggcagtaaca tgccgatttg
     2101 aaacggacga atcaaccgcc aaagccgatc attcttttgc aaatatttga taaacaaaat
     2161 actcgggatc aaagtgatga aaaacacagc ataatccact aaaaacaaaa ttttcacttc
     2221 ataaaagtga tgggctccgc tagcagaaac tgggaaatct ggcaagctga ggatggtttt
     2281 gaagggatta ttgagataat tcattaggtg atcaaaattt ttcagtaacg tctcttgatc
     2341 taaagaagta taatccaaaa tttgcaaatg tccgatatca aatacgtata gtggtcgaaa
     2401 gttgatcgtc acaccaactg ccagactgat cagtgtcaga aataggctga acaatccagc
     2461 atattccatc cactgccatc tcgaattttt catattagaa gctccattca tccagtgatt
     2521 gtttcacata ggtagctggg ataggcaacc caggtacatc ttcttctttt gtaaaacctg
     2581 ataaaacgag caaagtatct atattattgc gtatcccagc ttggatatct gtttcatagt
     2641 tatctccaac catgattact tcttcttttt gcagccctaa tacttcaacg gctttatcca
     2701 taatgatggc ttctggtttt ccgatgtaga tcggcggcgt ttgggttgct gtctctacaa
     2761 aagagatgac tgaccctgct ccaggaagaa ggccgcgttc agtaggtata ttcttatctg
     2821 ggttcgttcc aatgaaagta gcaccttttt gaatagcaag tgttgcttta actacttttt
     2881 cataggtcag ataattatct aagcctacca ctacatagtc aggagcctct tcttcccatg
     2941 taaatccggc agctaaaatc aagtcaatca atcctgcttc tccgataaca tagacttttt
     3001 tcccttttcc atctgctttc ataaaatcga ttgttgccaa agtagctgtg tagactgtct
     3061 ctggagctac atgaatgtca aactcatctg ctagacgatt agctactgtt tctggtgact
     3121 tagtcgtgtt attggtaaca aacaagaaag gtaattttct tttttgcaat tcttccacaa
     3181 atcgtttacc ggccggtatg ggttgtgtcc cacgataaat cgttccgtct aaatcaatta
     3241 aatatccttt atagtccatt catccactac tcttcccttt ttctgatcgt aaatgctgtt
     3301 tttgtttttt ctacttgttt aggttgttct tgttttttta ctttttcaga ggtttttgct
     3361 gactctgttt ttcgattttt aataaccggt tttttgcgat tagatgcgac tggtcctttt
     3421 ttctcatcga tatacgcacg attatggttt tttttctttt ttcggcgctg agttgtcttt
     3481 tcttttttac cgccgattct ctcgatgaca aaatacgcac aaccaaaatt gcaatattcg
     3541 taaagatagt cttccaagga atcgattcgc tgatctggat gcgcccgtcg attatccgct
     3601 tggaaaaatc cttttaggcg tagttgttca taaccccaat ctcctacgat ataatcatat
     3661 cgggctagta catcactgta acgttctcca agcttttctg cattaaatcc ttcacgataa
     3721 tcggacacaa gccgataatg tctttcacct atcaaaaaat tactgtcatc taacagtgtc
     3781 actagctcgc ctttgacttt ttctgcttta ggctcctcaa cgatttcttc caaagcagct
     3841 gtcagttctt cggtgactgt tgtttcctgt tcagctgatg attttttctt agcaatttct
     3901 gttttatgct caacgttctg ctgttcattt tgttttgcag acatgcgttc accacctatc
     3961 ttttttatta taccttgttt ttatttgatt gggtagtgag ctttcaagta atctcctacc
     4021 acacttcgga taaattcagg gaacaaaaat tcattttttc cagcaatctc aatcgttgga
     4081 aaaaatggaa caaacatcaa atgatctacg gtagtaaaag tataattttt attcagatcg
     4141 attggcttgt tcttccaaaa gacttgatgg ttaatagaat catatgtgat tccattatag
     4201 accatctgcc caaaaatctt accgcgaaat cccattccct tcataggata gttacgtaaa
     4261 aagttacgat ttttttctat ttctaaaact aagcggatca gatcatttcc acccaatgtg
     4321 acatttaaca agtgcatcgg atgcggcaac gcagtgtgaa gctgatcctg attgacccgc
     4381 ccttttggaa tttcagttaa aaacagacca ctgtttaata ttgctgcttc cgtctttcct
     4441 cgttctttca ccgcctcaaa ggctacttgg ataaacgaat gctcattgaa gatatctaga
     4501 ctcagagaaa atggcaaatc tgctacttct ttttctttta ataattcatg cccacgttct
     4561 agataactat ttatttcttg ttcatcttct gtaaaagctg tcattgtttc ggtagggatg
     4621 gctttggctg aatgcttaag gatttttttc tcttcatcga tggttaaatg gacttcaccg
     4681 acatactgcc cgtactttcc agcggctgct atttggacat cattgattac gtgaccttct
     4741 ctgaataaat gatgggtgtg cgatcctaaa ataacatcaa ttgctggtat ttcttttgca
     4801 atttggaaat catcctgaat cccgagatga ctcatcaaaa ctaaaacatc cacttgtgaa
     4861 cgtaaatctt cgactaattc tggcaaaata tcataaggat tccgtatgtc ccagccattc
     4921 ggattataag taagcggaaa tggagcagta aatgcaatta gtccgatttt cgtctggtgt
     4981 ttcgttgtaa tgattttata tggtttcgtc catgccggtg gctggagcgt atttttatcg
     5041 aataagttat ccaggatgat atcaaactca gcatgatcgt ataaatgatt caactcttct
     5101 ttggaatttc ctacgccttc attgtttcct atcgtcgctg catcataacc aacttcattc
     5161 atcagcaaga tattttcctg tccattcgtt gcttctgaca atggatgcca acgatccaca
     5221 aaatcaccta gatctactgc aatcgttgtc gttttttctt tcttttcgtt ttctcgttta
     5281 cgttgatcca aaaaccttct gatccttggc caattttcta aatgagaatg cagatcattc
     5341 gtatgtaaaa tcactatttt ttccatgtac cctctcgcct ctctttatct ttatcatacc
     5401 ataggcagct ttttcttcca ttgaaacaag ctctatttca gagtttttcc tgccttgaag
     5461 caaattgtat aagtacaata ttcgataaat cagtctggct aaaaaatcag gaacattcat
     5521 ttttacagta ctataaaaat aaaaaaaaca gacccgaaag tctgttgtcg tttcattata
     5581 atcagacaca tggcggaacc tgcttagtgc tgcttccttc cagacctgac acgcttcaca
     5641 agcccaccat tgtcctagcc tttcggcaaa gaatagtata ccttattttt cattcgttga
     5701 ccagtctttt tttaatttct tttttcgctg gcggagcttt cggaaaaagt cagtcaaaat
     5761 catcccacat tcgtcttcta aaatgccacc ttctacatat gcttggtggt taaaccgctc
     5821 gtcctccaaa agattcatca aggtaccggc tgttccgcct tttggatcat acgcgccgaa
     5881 ataaacttct gctacacgag acaaaatcat cgcgccgctg cacataggac aaggctctaa
     5941 ggtaacaaaa agtgcagcat cttccaagcg ccagcttccc actttttcac aggcttcctt
     6001 gattgcaagc atttctgcat gtgtagtagc atcttgcgtg gtttctctaa ggttatatcc
     6061 tcttccaatc acttttccat ccaatacaac cactgctcca atcggaactt catgaagcat
     6121 ttctgctttc tttgcttctg ccaatgcaag gcgcatccat ttttcttttt ctactgcatc
     6181 aagatttgta tttaccaatt acccttccct gcctttgttt tttcttgttt tactttagca
     6241 tatgataaaa aggctgtaac taactttttt ttctatggta aaataaatac aagaggtgaa
     6301 caagatgaaa atggataaat tacttgctct ttacccacaa gcacagaaaa aaaactctcc
     6361 agcatcagaa gcggatatcc tttcttttgc aattgatgat gcttttatct ggatcaaaca
     6421 agattcatta tctcaacaag aaaccagcct tttgaaagcg ttattccctg taatcaacga
     6481 tcaaaaaaaa cacccatggt atcactatct ttttaaagat gcttcttgtg ttcaagaaaa
     6541 aagctttcgt ttgatccagc ttcacgtcga atcaaaagga gaattcttaa aaaaagaatg
     6601 gcaagatact attttggaat tatttcgcta ttctgctgat ttcttttttt atacagacac
     6661 agatgcgctc ttagtcgaac aaataaaccg tgattatctt gatgctactg aaatcaacgg
     6721 tatttttcta tctttagatg ctgattttga tacgacaacg tctgtattcg ttggttcttt
     6781 ccattcacct ggcaccgacc tcgtcccttt gtttgctgaa gaaagaaaaa tttttttaac
     6841 agagcaaaac aatctttata ctcacagccg tactttttct atgcaagatg tggctttaca
     6901 ttactataca aaagatactg taaaagatag taagttgatc aaaagttaca tagaactgat
     6961 caaacaaaat gaaatggaag tgatcattca agaattatgg aatgaattag gcaatgtcag
     7021 ctctgctgca aaaacactgt ttcttcatcg taatactcta aaatatcgaa tcgaaaaatt
     7081 tcaagaacag tctggtttca atctaaaaaa agccaacgat ttactgtttt gctatctttt
     7141 attgcttcat gaacataatt aaagatagat taaaggggtt gtgacaataa ttttgtcaca
     7201 accccttatt tcgaatgaat agtgttcaaa agctagtttc tcttacatca ttttttcaac
     7261 aaaatgcatt agccggtctt ttacatcttc ggttgaatct ttgattgaat caccataaac
     7321 agagattttc ttttttcctg atttgatttt tgctaacatt cccatcaaag aatccaagtc
     7381 actatctgaa agaccatcta cgatttctag taatttttca tttccatcaa atttgtcata
     7441 gacgaatttt tttactttcg tccgagtagt catatgatgg atcttttcca ttacttttcc
     7501 cgatacaacg actgctgtcg ctacactggc agcggctgct acgcttaatc cgattgttac
     7561 ttttgttgat gttttcataa gtatcagctc ctttttatct atctccatca tagcatagac
     7621 aaaaaaataa acgaactaat ccactcagtg aaatctcaat cctttaggga aaaagttctt
     7681 tactgttcct tttacttgtt tcccaaatcc gacaggtatc ttatctaata ctagcaaaac
     7741 ccatccttga ttcccatcac gttgaaatgt ttctcctgct gtgtaagatt gccattcctt
     7801 ttgagtgatc ggcaagcaag gtatattctc tatctttttt gtagcaagcg ctaatgcata
     7861 gctaggttca aatcgattct ttttgaaatc tcctaaatgc aaacctgtac gtacgacttt
     7921 taaaccatcg agagaagggg ctaactcagg aacttcccaa agatggtcgt tgaacactaa
     7981 aagtcttcca gtagcttcat aatgaaaatc atttgaaaac tccgtccaga gtttttcctg
     8041 ctcttttgtc atttgcactt tacttttttt tcgtgtcttc ttttctttat gcatctgatt
     8101 ttgtccatga aaagtcagct ttgcaacaaa atgtccttca ccctgatctt tatgaggcca
     8161 tatacgtatt gttttttcca aaccagctac gcttccccat tcagaacgtc ctgaactaac
     8221 ggattgagtc aaaggaatct cctcaatcgt tactggataa ttttctacaa gccagctaat
     8281 gatctcttca ttttcttctg gtgcaaacgt acaagtcgaa taaatcagtt gtcctttatt
     8341 ttttagcatt tttattgctg aagacaaaat ctcttgctga cgtttttgac agtatagcgg
     8401 agattcttct gtccactctt ttattgcatt tggatctttt cggaacattc cttctcctga
     8461 acaaggagca tcaaccacga tccgatcaaa aaagcccgaa aaatgaggga caagttctgc
     8521 gggtgcatga tttgtcacta ttgcgttcga aactccccat cgttctatat tttctgataa
     8581 aatcttcgca cgtttcggaa agatctcatt cgtcactaat aaccctttcc ccttcatttg
     8641 agcagctaat tgggttgatt taccaccggg agccgcacac aaatccaaaa ctttttcacc
     8701 gggttttgct gcagcagctg ttccaacgat cattgcactt ggttcttggc tatattcata
     8761 cccagcttga tggaggaaag actttccatt gacagtgcca agaaatccct cattgctgta
     8821 gggagcgggt tgtaattctt cgctatggta agtttggacc atgtccaatc ctgccggttt
     8881 tagtggattc caccgaaatc cttttttcac gcttccttgt tcaagcgcag aaaaaaaatc
     8941 ggatgcttct tcgcctaaaa gaagacgata ttttttgata aattgctgtg gtaacgttgt
     9001 cgcttcctcc ttcattccaa tgtttcgtta gacattttac aatctttttt acgaaaaata
     9061 aaggtggctg atctactttt tcatgttgct gcctaaggtg actgttcctt taagatattt
     9121 tccaagtatt catccgtcac ctcgttttta tcccaaacag cgttcatcat ttcaagaaaa
     9181 tcttgagaag aatcatctga gcgcaaactt cccaatgcat taagccatac caattcaagc
     9241 gacttttctt ctttgatctt cgtgtgacct ttgacatatg agaaaaatgg ccgcttaaat
     9301 agcgaatcca tgtctaaatc agattttgca atgatttcac gaatactttc atggatagaa
     9361 aaaaaatctt tcaataagta gacaatgctt aaaacatcat cggaactgca ttcagctaag
     9421 tagttttcaa aataaacagt gatttcttct aatatcttct cttttaaccc gctgattgat
     9481 ttgaaatgtg tataaatcgg gtatgtagaa caaccgcaga actctgctaa ctctcttgca
     9541 gtaattgact tcattccata ttcttccacg tataccatac ttttgaacag caaatattct
     9601 ttactataat ttgttactct ccccatcatt cttacctccg tctcttttca tctacccata
     9661 aataaataac ataaataatt ctaaaaatat tattgataat tatcaaaaca tatttagtta
     9721 tatccattaa aaatcaattt ttccgcctta aaaacaagaa tatcttgaca aaatacccta
     9781 ggaaataaac aatagaataa acaaggtaac aaaaaaatta aataacgtta tataaaaaag
     9841 aaggcgacac tctgtgagaa taacacagac tgaaatgact ttccactcac agtttttgtc
     9901 taactatata caaaaacaga ataaaaagga ggtatttatg ctaattgaag caagaaaaaa
     9961 agagttactt gttctacaaa tggtcattca aaaaaataat tgttctttac aagaattatc
    10021 caatgactta catattccaa aacggacaat caaagaactg atccgaaaaa taaatgtatc
    10081 actcaagcaa tttttattga ttgatgaatt tatttattct aatcataaag gagagatcca
    10141 aaccaaagaa accgatcaaa ctaaaaagtt agctgttttt tctaagttaa aattatgcta
    10201 cctaaaagaa tccaatcgtt ttaactattt gatgttacta atcaattatc cacaaactgc
    10261 tgtaccaaaa aaatatttac ttgaacaact ttatatatcg ccctcctatc tcgaaaaact
    10321 gacaaaacag ctcaatcatt ccttaaaaaa attccattta cggatcctat ctgtaaaaaa
    10381 ttgctgtact cttgaaggaa atgagttatc tatccgtctc tatctttact tttttctttc
    10441 agatacattt ggaggtaacg attggccatt ttcctcactt gaattaaatg cactaaaaat
    10501 gaacagtaca gatgagcaaa tagaaaaaat ttcaaaacaa gaaaaaaata tctacttgct
    10561 gttaacttta ttcatattac gaaacaaaca acaggcatat tttccaaaaa cttcttctga
    10621 aatcactgaa ttgatggact tattgcagca tacacatgat tattcagaat actttacttc
    10681 tttttcagtg agtcgccctt gtgaagagac agaaacagag agagcctact ttaattattt
    10741 tttctattgt ttttttccaa atcaaatctc ttttcgacaa aaacaggaga ttggtttttt
    10801 attttccaca agcaaaaacc ttatttgtcg acaagtcggt caattgatga cttacttaac
    10861 tcaacagttt cctctcaacc atttagaaaa taaaatacca atttattttt attttcttgt
    10921 gttggcagtt gtttttttcg aactgttggg tgaatccagc agtagttttt ctacactttg
    10981 ctttccagaa gccacgtcca atcattctag ttcttttata caggaaaaga aattaagaga
    11041 aaaaatcgat acaatttttt caaaaaatgg ggaaagaaac caaatcagtc aataccttag
    11101 tcaattgatc caacacttac tacaaacaga aacgaaagag cagttactca tccatttgca
    11161 gataaccaaa cagtttacag gaaattatca tcttcaaagc catttgcagc agttatttcg
    11221 caaagatgct gttaaaatta ccgcatattc agaacatgct gatttgatca ttacagatca
    11281 gcatgagaaa atttcagatg aaacggagac tttctatctg gatacaacgc acaatacagt
    11341 gttatttggc aagttaataa aagcggttca tgaactgtat atgaaaaaag gttatgagaa
    11401 caaaacaaag aggatgtgac aaaattcttg tcacatcctc ttattccaaa taaatggtat
    11461 tcaatcagct aactctcggt attagctcag atcaaagcgc tgatttcaca acttctttat
    11521 tttaatctct tcggttgccg ccgaacaata agatcaagcg caacagctgc aggaatgtcg
    11581 aaagcgctgc tgcaacataa gtcagtgctg ctgcaaataa tacgtgtctt gccatcggta
    11641 cttcttcttc cgtcagtagt ccgccttcac tcaaaatttg gattgctcgc cgagacgcgt
    11701 taaattcgac tggtaatgtc accaattgga aaagtaatgc taaagaaaaa gcgaaaatcc
    11761 cgatattgat taacgtagag ttccagctaa gcaatacacc taccataatc aaggggaacg
    11821 aaatcatcga accaaagtta gctgctggaa caagtgctgc acgaatcttc aatgggacat
    11881 aacccgaagc atcttgtacc gcatgtccgc attcatgagc tgctacacct atcgcagcaa
    11941 ctgaagtaga ctgtgcagtt gcttgagaca aactcaacat cttattacct gaattgtagt
    12001 tatctgtgag atctccggca atttgttgaa caccgacatt atttatctgt tgactttgta
    12061 atatatattg tgctgcttgt gtaccagtca cattgttttt gcttctcact tgatcgtatt
    12121 ttcggaaagt actattgacg tacgctgacg caattccgga aataactaat ccaagaatga
    12181 ccaaaagaaa tgtcggatct aaaaaaccat acattccata accatacatt ttcgtttcac
    12241 tccttttcgt ttactttatt ttatcattag tagtaggaaa agaatgtgaa aagaccttga
    12301 atattcttta attctttttc acaaattccg atttcagttt catcggtcca aatccttcta
    12361 ccttgcaatc gatattatga tcgccttcta ctaaacggat gttcttgact tttgttcctt
    12421 gtttaatggc acctgacgca ccttttactt tcaagtcttt aatcactgtc acgctatcgc
    12481 cctctaacag acggtttcca tttgcatcta ataccactaa tccgtcttct atgacagttt
    12541 cttgttcttc tttgttccat tcgttcccgc attctgggca aatcaacaag cctcgatctt
    12601 catatgtgta ctctgagcca cattctgggc aatttggtaa tgtcatcttc tttctccttt
    12661 aaaatcttta gtgagcacta ttgtaacgga aaaagaagat cttgaaaaga tacatttgtg
    12721 atttttacta caaaaggcac gttttcgaac gaagtatcat tctgtttttc tgctcttata
    12781 cgattggcat atatcttgga tctctttata gatttcctct acttctgcta aatcttttgc
    12841 attcgcacca cttgctaatg gatggcctcc tccatgatgg cgtttagcca attcattgat
    12901 gatcggcccc ttagaacgta aacgtacgcg gtaataccct gaaggctgct gaacaaaaat
    12961 cccccaagcc agcacttcgt caatcactcc aggaagtgaa acgatcgaag ctgtctctga
    13021 atcatcgatt ccatatttat caagaatttc ttgcggaagg attattcttc ctgcaccatt
    13081 ttcatccacc tggatatttt gataaagata accggaaagt ttagcgactt tcatcggcat
    13141 ttgttcaatc tctctgttca aagcacttgc atcaaatgga taatttctca atatcgaagc
    13201 aatctctaat gtacgggacg tcgtggaggg atacaaaaag cgccctgtat ctccgacgat
    13261 tcctgcataa agtaaccgtg cagccttaac gttcatcttt aaagattcag gaaacatcaa
    13321 agcaaattct gcaatcatct cactacagct acttgctttt gtatttaccc acacaagatc
    13381 tccataaggg tcatcattgg gatgatggtc gattttgatc agtttttctg ccaactgata
    13441 tctttgatca ctgattctgg gtgaattagc tgtatcagtc acgatgacta gcgctccttg
    13501 gtagaagaca tcttcaactt cttgcatttc tgctaaaaag cgaagccctt caatcgtttc
    13561 acctacttga cgtatttctt tttctgggaa actttcccgt aatagttcgg ctaatcctac
    13621 ttgtgatcct aatgcatcag gatcagggcg caaatgcctg tggataatga ttttatcgta
    13681 tttttttatt gtatctacta tttcttgctg tactgtcatt tcactcctgc tttctaagaa
    13741 cgttccatta cttgacaaac aacgattgct ttggaaacga tggtgttttc aataaacact
    13801 tcgatatcca gtttagcgga acgtctgccg atttccaata cacgtggacg aatgtctaat
    13861 tcgctttcca attggatcag ccgcaaataa tgtaggttca cttgttcaat caagatattt
    13921 cgtttttggt tcatcaccat tgttttttgt gcgacatcag aaatgatttc gctaagtacg
    13981 ccaaaagaaa tggttccaac gccattgacc atttgtggtg ctactacgaa tttgaactta
    14041 gggttttcca aacgatttcc ttcacggtca gtatcaatcg tatggatatt tccagatatc
    14101 tgatccgaaa tcgtatctgc aatctgcggt tgtcgctgga caagctgcat cgctttcatt
    14161 acatcttgcc gggtaatcaa gccttccaat gtcaagtcat cggaaacgac cggcatgact
    14221 tctagcccat cccagatcat ttgatggcta acagaggcaa tactcattgt tttctttaca
    14281 ctgattggtt ctttcgtcat cacccgatcg atgatctggt ttggcgattt ccctaaaaca
    14341 tcttttgccg taacaatccc catcaaacga tgatggcggt tgacgactgg aaaacgcgaa
    14401 tgctgcgttt cttctgacaa acgctgataa tcagaaatcg tatctgtctg ctgcaaataa
    14461 tgagtctttt cagctggcat atagatatca ctgacaagca tgatatcttt tttgatcaat
    14521 tggtcgctta acgctcggtt gatcatggtt gcgactgtaa atgtatcata agaggtgcgt
    14581 aagatcggca tttcaagatc atcagctagc tttgcaatct cagcagtcgt attgaatcct
    14641 cctgtgatca atactgcggc gccatcttcc aatgctaatt tttggacttc cgcccggttg
    14701 ccgacgatca ttagtgatcc tggcgtaata tagcgttcca tggcatgttt agtcatcgcg
    14761 ccgatgacga atttattcaa gaccttgtct aatcctgccg ccccgcctaa aacgtcccct
    14821 tcaatgatcc tgacgacctc gccaaatgtc aaacgttcaa tatgtttttt taatttgcgt
    14881 tcgatacgga tggtccctac tcgttgaatt gttgaaacta gtccgacatt ttctgcatct
    14941 ttgattgcac ggtacgctgt tccttcactg acattcaatt cttttgcgat gcttcttaca
    15001 gaaattcgat tgccaacagg taaactttca atatattcta aaatcaaatc atgttttgtc
    15061 gccattttct gcctacactt ccagcgtttc cgagacgctc attatttttc cttcactatc
    15121 ttccagtaac tctacaaaag attcgggatc ttgttcaatg acagggaaag tattgtaatg
    15181 gattggaact actttcttgg cacgaattgt ttgcgcagcc tttgctgcat cttctggccc
    15241 catcgtatag ttatcgccaa tcggcaagaa agcgacatca atatcgaatt cttctgcaag
    15301 caatcctaga tcactgaaat aagcagtatc ccctgcatga taaatcgttt ttccttctgc
    15361 ttgaataata attccgctag gctcgcccat atactgaacg ataccgtcta cttcatagct
    15421 tgaactatgc tgtgcatgta cgaattttac cacgccgaat ggaaacgcat gttttccacc
    15481 gatattcata ccgaatgaat ctactcctct gctgttcgca taatcagcaa tctcaacaat
    15541 actgataatt gtggcttgat tttcttttgc aataggaatc atatctccta tgtggtcact
    15601 atgaccatga gtaatcagca accaatctgt ctttacttct tctgcagtta agtctatcaa
    15661 tggatttcct gtaataaacg ggtcaatcat cacgcgttgg ccattttcca tttcaattgt
    15721 taaacaggaa tgtccatgat acgtaatttt catattttac acctcgcgtt ttctctgtat
    15781 cttttttatt aaaaacgatt tcatcacttg cacatcaatg aaaaaatata gctgctaaat
    15841 atattttcat tggattgata gtacagtatg tacaaagcaa ttgtaacaga taatacagga
    15901 tgcaccaagt accaagcaga tataatctca agtttgaggt ctctgctctg cttagcttct
    15961 cttctcgtcc cttaactaat taaatcagcg taaataggat ggacgatctt ttctaaatcg
    16021 gctactttta tccgaaggct tctcccgatt tcaccgcttg acacaattac ttcttctttt
    16081 tcttttaaag aactgtctgc taaaaaacaa taatcaggat gtgcgagttt tattccaata
    16141 ggggtgtttg ctccgtgggg atatcctgta tgcattaata caaaatccat tccaggaaaa
    16201 ccaactttac gatctttcgt cacttgtctc gtttttttgt aatctaagtg atcgttcaaa
    16261 ggaatcaatg cgatgatcac cccagttttg tttcctttca ctacgagagt tttacatatc
    16321 aaagctggat caatccctct tttttgtagt tcttctgtga aagattcttc taccacatga
    16381 ctaaaatcaa gtggttgata gctgatcttt tgttcttcta aataacgttc gacttttgtt
    16441 tgactcatca ttgattcact ccctttattc tatcgtttat tataaagcga gggatgctgt
    16501 attcaaaaat tatttgtttt tcgcaaaaaa agagcaagac gagcactcta tagagaggtt
    16561 catcttactc tttttgttta ttttttattt cgacgttgag tagcttccat aatgactggc
    16621 aagatcactg ggcggcgttt tgtttgttca aataagtaac ggcctaattg atctcggatg
    16681 tcttgcttta atttgctcca ctcaaaatca ttgctttcta aatgtttctc aacgattgtt
    16741 tcaatcatat tgctgctttc tttcatcaga tcacggctag cttttacgta aacgaatccc
    16801 cgtgaagtaa tttgaggttt tgaaatgatt ttcttttctc gccgactgat agtgacaacc
    16861 gctacgaaaa tcccgtcttc tgacaagatt tttcgatcac gtaaaacaat gtttccgata
    16921 tcacctatgc ctaatccatc aatcattaca ttttctgcac ttgtagttcc tgcaacactc
    16981 atacgcttgt tcttatattc cagcacgtct ccgcgtccag taatatagat atttttgtat
    17041 ggcatcccta ctgcatgagc caaatctgca tgagcagcta attcacgata ctctccttga
    17101 actggcacaa aatatgttgg tttcatcaaa ttgatcatta attgcaagtc attaggattg
    17161 gcatggccag aaacgcgcat attctcagag atcaacttca ctgttccacc agctcgataa
    17221 atgatatctt ccgttttcgc cacgatggtt tccatcgcga tgcttggcgt agtagtgata
    17281 taaaccaaat ctccttcttc aatcttgaca ttacggtgtg taccgtttgc cattttttgc
    17341 agagacttga tgggttcacc cattcggcca gtttctaaaa tgatcagttc ttctggatca
    17401 tagtttttca tgtctttcaa cgtaacaagc agatcttcac ttggtaattg aagtttttct
    17461 aaacgcatcg ctgtgcgaat aattcgttcg aaatcttgac cagtcaagac aacttttcgt
    17521 tgtgcttttt ctgctgcatc caatacttgt tgcactcgtt gcaaattact tgctacacaa
    17581 gcgacgatga tacgcccttc ccaatatttg atcgtatcaa atacttcatc tgctatttgg
    17641 gcttcagaag ctacaggctg aggattttct gcatttgccg attcacttag taaggctaag
    17701 actccttctt tgccgatttc cgccaatcgt gcataatctg tttgatacat tgggatagcc
    17761 gtttggtcaa atttaaaatc tcccgtataa acgatactac cttcttttgt tttcagattg
    17821 atccccactg aatctggtat cgtatgagtg gtacggaaaa agctcactac tgtttcacca
    17881 aagtctattt ctgtatgctc atctaccaca tggaaatcct taaatccttt ggctgcttca
    17941 ttacgactga cattcaattt tgctaactcg atcgtcaact ctgtcccgaa tactggtaca
    18001 gaaatttttg acaacaagta aggaagtgcc ccgatcgcat ctgcatgtcc atgtgttaaa
    18061 aagacaccag ctacacgatc aatgttttct tctaaatacg taaaatcagg gattacgacg
    18121 tcgatcccca acaactcatt ttcaggatat tttagcccac aatctaaaat aaaaatatcc
    18181 tcgcccactt ctgcgacata catgtttttt ccattttcac gaacgccgcc taatgggata
    18241 attttaatgg tactcacaaa cttcacctct tcttatgaca tccaagttta aagatgtcga
    18301 ttttcttctt tatctaaata tatgagccat tccaatgaag catatgcaaa atcactattt
    18361 ctcaacttat ggagaatcag caggaaaatt accagcacat ccgcgttctg acagttggat
    18421 gggcatttta ttttggatat acttcattaa caaaatggat cttttgcgaa acgctatagc
    18481 cagaatactt atataagtat tcagctgttc cccattatta tacaacaaat aaactaaatc
    18541 gctagtatca gcaattgtta gaacaactat gcacgtaaaa atccctctgt agcatctagg
    18601 atttcttttg ctattcctct ttcatttctt cttcgtcggt ttgaaacata cttttttcat
    18661 tttaccacat tttttgcaat aactatagtg actttttata tatacagaac ctcatgttga
    18721 tggtgtgcaa acagcataag atttaagcta gctttaagat aggttgtcta ttatcaaatg
    18781 catctactag gagggatgca aaatatgaat tttatgaaac gtgcatggaa aagtacaaaa
    18841 gtaaaatggg gacgttcatt gttattgttt gccgtgttca cagccattct tatttttgtg
    18901 ctagctggcc tcacgattcg aagcgctgca gaagaagcag cgaatcaagc acaaaaagaa
    18961 gttggagcaa cagtgacttt aagtgccaat cgagaagctt catttaaaaa acaggaacaa
    19021 acttcttcaa gcggttctac cactgaagaa cgtccagatc caggcagttt ttctcttaca
    19081 ccagtttcct taacggacgc ggaaaaaata gctgaattag ataatgtaaa gagttattcc
    19141 tttgaaagtt ccgcctcagc tatggcaaaa gatggaataa ctgccatcac tagttctgat
    19201 tcaagtgaag acagttcaga aactactgat gcaggcggtg aacctgctgg aggaatgggc
    19261 gggaatccgc aaatgactca agcagacttt caaatcagtg gtgtgtcagc aactgcacaa
    19321 aatagtaatt tttctgatgg aacagcaaaa atcacggatg gtgaaggaat agatgacagc
    19381 gacaaaggca caaacaatgt agtcattgat tctactctag cagaaacaaa tgacctggca
    19441 gtcggagata ctttcacaat cacgagtact gaagatgaag ataccactta tgaaatgacg
    19501 atcaaaggaa tttatgaatc cagcgaaaca agcggcagta tgggtatgaa attcaacttc
    19561 atgaatccgt cgaatacact ttacacttct tacacttttg caaatgaact aaatgggaca
    19621 tctgacgata acacgataga ttctgctatt tacactctat ctgatccaga taaaatggat
    19681 gagtttctca aagaagctga gaagctgatc gacacagata catttagctt acaatcaaat
    19741 gattccatgt atcagtcaat gttggaacct ttaaacaacg tggcaagttt ttctaaaaat
    19801 gttgttttac tagtggctgt agccggtatt atcatactta ctctgatcat tatgattacc
    19861 attcgggagc gtcggcatga gttaggcgtc ttgctatctt taggagaatc cagaagtaaa
    19921 atcattctcc aattgtttac cgaagtggct atttgtatga ttcttgcatt aggagttgct
    19981 tcgcttagcg gaaacgtcgt cgcgaatgcc gtgggacagc agctacttga ccaacagaca
    20041 gaaaccacga cacaaaacca gcaagctcct ggcggtggac aaatgccagg taacagacaa
    20101 gaacaaggcg gtcaacgcga tggtcaacaa ggaaccacga ataatccatt tgctgtttct
    20161 gatcaagtca gcgaattgga aatcaatgtc aaacctgcac aattgggact attagccgga
    20221 gtagcatttg gaatcagtct gttttctgtc tttttagctt ctatagggat attaagatta
    20281 aacccaagaa aaatcctatt aaactaaagg aggaatttga tgttacggac agaacaattg
    20341 ggattttggt atcaagatcc gcaagatgcg ctttataaag atgtagaact gaatttcgaa
    20401 gctggaaaaa tgtacgctat tttaggagca agtggttctg gaaaaacgac ttttctttcg
    20461 cttatttctg gactagatgc accaaaagaa ggaaaggtat actatcaaga tcgttcactg
    20521 aacaaaatcg ggttaagaag ttatcgtaaa aatgatgtct cgattatttt ccaagcttac
    20581 aacttgcttc cttatttatc ggctctagac aacgtcatag cagcaatgga aatcactaag
    20641 accaagcaag agaattatcg acaagttgct ttagatcgac tggctcgtgt aggaattgat
    20701 gaaatattag caaaaaaaca ggtgaccaaa ctttcgggag gtcagcagca gcgagtcgct
    20761 atcgttcgtg ctatctgctg tgaacatcaa ctaatcgtag cggatgagcc tactggaaat
    20821 ctagatgaaa caacttcaca agatatcgtc aaacttttcc aagaaatcgc ccatgaacaa
    20881 aatcgctgta ttatcatcgt gactcatgag attgaagtag caaaaagctg tgatgaagtc
    20941 tttgaactaa aaaataaaaa atttcaaaaa atcgaagttt aaaaaacagg ataatgatca
    21001 aataaaaaat ccgaaccata tctgtctcgc agatatggtt cggatttttt atttgcttga
    21061 ttcaattagt aataagtggt ttttattttt tttcgtcttt tcgagaaaac ttgtctttga
    21121 cttcgtcgcc taaatttcga gctttttctt tgatgtcagc acctagtgat tgtgctttcc
    21181 cttctgtttc tttgctcttg tcgtctgttg ctttgccgta tgcttctttt ccttcaccga
    21241 ctactttatc tttcatatct ttagaacgtc cttttaaatc tgccattgtg cttcctcctc
    21301 ttagtttgtt tattgatcta ccttcagggt aacatcaaca acagatacag acaaacagaa
    21361 cgctcgtttt ctaaaaaaga tagttagagc tgttgattaa aaactttttt cgaaagtccc
    21421 ccatactttg acttcttttt cttggaccat tatttctcct ttagcgataa ctgtatcaat
    21481 atcaagcgtt tctttatcca agatacacag atctgcatca gcaccaattt cgatttttcc
    21541 ttttttatcc aatttcaata tacgtgctgg attactggtt atagctcgta aggcgatttc
    21601 caaagggata ctttcttttt gcacagcttc tttgatccct actagcaatg ccttggcact
    21661 tcctactcct aaccctagga aatgattgtt ttcatcaaaa taaggcaagc ttccttgtcc
    21721 atctgaggtc atcgtgaaat tatctaaaga aacatcttcc tcgatcaatc gtttcagtgc
    21781 tttgctgaag cgaacttcac catctgtctt ctcccaaaaa tcaggatctt cgctagcagt
    21841 gaagtcaatc gtgccaccac gtttggcaaa tgccacgcaa gcttcaaata attctggtgt
    21901 gcgatttgca tgagtaggca aaaattgtgt gatcggaatt tctgtttctt ctacgattcg
    21961 tgtcaatagc tctagctttc tttttcctcc gcctaagtgg acattgatga tcccagcttt
    22021 tccggataac atgcctccta cacgagcatc tgctactgca cgagcgaact cttcaaaact
    22081 tggctgagaa gaacggtgat cggatactgc gatttctccg attccgataa ttttatcaat
    22141 tgtcaaaaag tctttgatga tagaatctgt tactgttttt accggcaatc ggtacgaacc
    22201 ttcgtataca taagtggaga ttccttctgc ttccagacct ttcgctttgc tgatcaacgc
    22261 agtcatatct ctaccttctc cgtctgttcc caaacaaccg acaactgtcg ttacaccagc
    22321 tgtagtcaat tggctcaatg tgacttctgg cgttcgattt tgataaccgc cttctcctcc
    22381 tccacctaaa atatggaaat gacagtcaat gaaacccggg gttagtactt ttccctcacc
    22441 tgaaagttct tctacttcca ctccttcgta gccgccttct agctgttctt ctacagccaa
    22501 aatcttccca ccggcaatca gtacatccat ttttcctaag taatctggtg caaacacttc
    22561 aatttgtcga atgattttca tacatttttc ctctttcttt aagtataact gatgatagga
    22621 tcttaaaaaa taatccaaac gataggatct ttactccttt gattttaccg taaaatgacg
    22681 gttcttggag tccttatcta ctgtttggat tactttttta tttacaggtc tccagtacta
    22741 ctgcagacct attgcaatgg caatcatgat gaaaatagtt cctaaaacaa acaggaatcc
    22801 ttgcatcttg atttggaatc ttgcccattt tgcccattcg atccgtgcaa cacctagcac
    22861 accaatcaag ctggctgata cgggtgtaaa agcgtccata aaacctgaac caagctgata
    22921 agcaagaact gctacttgtc tggatacacc tactaaatct gccagtggag ccatgattgg
    22981 catagtcaaa gctgcttgtc ctgaatttga tgtaacaacc aagttgaaca aactttggaa
    23041 gatgtacatc gcccaagcac caatgacagc tggaacaccg gtcaacgccg tcccgatccc
    23101 atgcaaaatc gtattcaaag ctgaaggaac agatgcatca gatcctccca agacaagaag
    23161 aatccctttg gccataccta ctacgattgc tgtaccagct aaatcagctg caccagattg
    23221 gaaagaagaa gcgatgtcat tgactttcat tccatctaat ttgaaaagaa cagcaatgat
    23281 tccagcaact aatcccatca caaagaattg agaagcgatt tctggaatgt aatagccttt
    23341 ttgagtcacg ccccaaacga tccagacaag gacaactaac atctctaata agatcaattt
    23401 atgcccaagc atgaattttt tattttcatc agatgtcttt ttgatatgag aacggaaata
    23461 atcatctgat ttgtaagtca acgaactttc tggtttttta cggatttttt ctgcatatat
    23521 catcatataa ccagcggcta atgcagtaac aactacccac ataatcaatc ggaatgtcgc
    23581 accagacaac acggggattc cagcaatccc ttgagccacc gctacgctaa acgggctcat
    23641 ccaagaggtc gcatttccga cttgagatgc gacataagtc acggttactg ctacgatcga
    23701 gtcatatcct aacgcaatga caaatggaac catcaccatt gagaatggaa ttacctcttc
    23761 tgccatcccg aatgtagcgc caccgaatga aaaagcaaag aataatagcg gtaaagctaa
    23821 acgctccagt cccttagttt tactgataaa cgcatagata cctgcgtcga tcgctccggt
    23881 acgcataata ataccaaaag cacctccaac aacaaggatc aatgcagcga tccctacagc
    23941 agatccatac ttatctccag atactagtcc ttcaaaaaca aaatttaaga aaccaaagcc
    24001 gccaaaatca tctgttcccc atattttggc tgttttatgc aattttttag aagtatcata
    24061 gatattttcg ccgtactgat cgtaaagcac atcatctgtc aaggagattt catctagttt
    24121 ttcctgcgta agattttttt ctccgtcagc aatgactttt tccaatcctt tttccggaac
    24181 atccagtttt tcacgttctg ctggatgatc ttgcagttct tctaattgat caaacacata
    24241 cgacttatcc agctcgtaag cataacggaa ggaatcttga cgtaaaacag tacgagtact
    24301 tgtttcgcca tttgcatctt tgtattcaat atcttgcgtg ctgaactttc ctgctggaac
    24361 gacaaaagtc agtatccatg caaagatgac tacaccaaaa ataatgacat acgtatgggg
    24421 tgtcgataat ttagtgaagt cgcgtttcgg ttttcctgaa gaatttgttt tcttcaaatt
    24481 tatttcttcc tttctttttt cagtaatttt gatgaataaa aaatggataa tcaaatgaat
    24541 tatacagtaa aaatgacatt tccacatgaa taatttatta gaaaatcact tttttctatt
    24601 ttgttttttt tctcctatgt atgattattt ttttctcttt ccatttatat taagtatttt
    24661 atttctctgt ttttccttaa aattcttttt ttatttttag attagagaaa atctcgtcct
    24721 gtttttttca ttttacaatc attgaaaaat tgaatttcat tgcaaaaaaa ataataaaca
    24781 cttataaata tccattatct atccttcaat ttttgtgtat tttatttcta ttagaaaaag
    24841 gtatcctgtt ggacaatttt gttatatcag gtaaacttca ttgcaataat gataacaaat
    24901 gtactataat tactttgtaa agagttttgg ggaagactct ttcatcaacg tgtattaaga
    24961 gacaagaaag aatccatatg gattctttct tgtctctttt tccactactt tttatttttc
    25021 tgacactaca tattcttata actcatttct cataactgtg aaacaactta taaaaataac
    25081 aaactttaaa caaaacaagc ttaaaatcaa agatgatcca tcttaaaata actaaaacaa
    25141 aaaaagtagt cgttgttaat cctcattaat aatcgttttc tttttttcaa acgctccatt
    25201 tttatgtgga acattatttt tttaaggaac tttgtcaaca tgtggataac tattttccac
    25261 ttgtgtcctg gactagctgt tttgctgtaa aatacaaaac ttgctgcagc aaaaagatcg
    25321 ttgcaagaga ggctgttccg cagatcgctg gatctagatt tggcaaagtc aagactgcat
    25381 catgtaaatt ggcgtggttg tccaaaacca aatcagcaaa ttcaaataat cgcaaaccac
    25441 tgctatgttg cgatttgata cttcgtgaca aatcaaatcc agtaacaatg attaatggat
    25501 gaccattttc tttgatccac tgagctagtt cgatgattgc cgggtttctt ccttcattcg
    25561 aaagaagaag aaaaatgtct tctgatttga cagaaagctg atcaataatt gcctgtcctg
    25621 caccttctag cgtttcatag acacctccgg ctggattttc aatcaatata cttggtacaa
    25681 gatccggggt ttgatcccaa aattcaaatg ctacaccact tagtgtgcgg ctagcatata
    25741 tctgcaatct accaccttga ttaatttgtt tcgcgatcca agtgccagct tgaagcatca
    25801 gcggctcttc tatccttaat ttctcttcca attggtgata atagtcaaaa taacataaac
    25861 tcatttatct ctcccgccta gctatacttg acatgaatag aaacagctgc ctaatctaag
    25921 gaagtacata aaaaaacagt agttagacag taacacatgt ggtctttgct gtttcaaaca
    25981 caaaaaaggc attgaacgaa tacgcccaat accttgacat agcaaataaa acaacgtgca
    26041 gaccatggaa aatatctaca taggggtaga attttcaccc tagattaggt acaataaatt
    26101 aggtcgatgc actgcattga ctatggcaaa atgatcggtc agggtgtatg tgtgtgactg
    26161 gtcattccct gattcttagc gccaactaag ggtcagggag ccttttttta ttcagctgtt
    26221 tctatctgaa tttattgtaa gtaatctctt atccaagtgc aagcaagaaa atacaaaaaa
    26281 acattggtat gatttatata tatataaata acataaaaaa aggaatggct tatcccgata
    26341 caacaagcat tcctttaaaa aagattcttt tatataagtg aaaaaagctt ttttccacca
    26401 attaaataaa tctctcatct caatagttta tccattattt tatctttttt tgcgtatacc
    26461 tactaaatat taccgtcttc ttctcgttct tctactttgt tcgagagaat ctgtactaga
    26521 aaaagtagtt gtatgtctac cttttctgcc tatccgagac tttcttttca tatatgggat
    26581 ataaatgatt agatacagta cagcaaaaaa taaagcaaat acgcaagctt cccaactacc
    26641 taacgccaat tcaaccatca aggtagcaac cccaacggat gctgcatacc agataaaaat
    26701 cagaaacgtt tctttttttc tgaccatgcg aaacatgata aatagactaa taagcaatag
    26761 tggcaacaca aatatccaat tgcttccttc catcagccac ttgagtatat caagcagcga
    26821 ctgtgcaaca gcgactaatt ttcccatagt gaacacacac ctttttttat aattaaaaaa
    26881 ttcgcctttt tattctacta tgaatcttct ggcttggctt tcatttctct tatgtaatca
    26941 ttttctttta tacaacttaa aaaaaaatta aaccattagc agaatatttt ctgaatctat
    27001 tcacgaaagt ctttttctat ctttcgtttt ccacgactca actgatctag agtcaaaaaa
    27061 ctaaaacgac agttgcgcaa atgacgcaca agctgccgct ttaataacaa atagcctttc
    27121 attctcagca ggccacttat ttccagttaa ctttctgttc ttagcttttt actcaactgg
    27181 ataatatatg cacttaatga agtcccaatt tccgaatgtt gaagtccata ttcaatattt
    27241 gtcttcatat atcctaactt atcgcctacg tcgtatcttt ttcctttaaa ctctctcgca
    27301 tagaccgcct ctgtctggtt aagagtctga agtgcatccg ttagctgtat ctcgtttcct
    27361 gctcctggtt cttgagtttc taatatctca aatattttag gagtcagtag ataacgtccg
    27421 ataatggcta aattacttgg tgcttttccc atttcaggtt tttctacaaa cccttttact
    27481 ctgcaacgac ccttttcgct atctgtttta ggatcaataa ttccgtattt agatgtttca
    27541 ttttctggta cacgcatgac ggcaacagcg gaagcttgtg tttcttcata atccatcatc
    27601 aattgttgag tcaatgggat atcatctttc atcaaatcat cccccagcat gacgacaaac
    27661 ggttcatttc ctataaatgc ttttgcttga agcacggcat cccccagtcc tcttggatga
    27721 gattgacgga tgaagtgaag attgacttga gtcgtttctt caactaaagc gagtaattct
    27781 gttttttcct tttcccgcaa attgttttcc aattcgatat ttgcatcaaa gtgatcttca
    27841 atgggtcttt tttcttttcc cgttacgatc aagatgtctt cgattcccga atccaatgct
    27901 tcttccacaa taaactggat cgttggttta tccacgattg gaagcatttc ttttgccatg
    27961 gcttttgttg ccggcaaaaa acgtgttcct agcccagcgg caggaatgac tgcttttttg
    28021 accttcactt
//
LOCUS       DACDYI010000039        27834 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.039, whole genome shotgun sequence.
ACCESSION   DACDYI010000039 DACDYI010000000
VERSION     DACDYI010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27834
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.039"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..540
                     /locus_tag="IWM52_001574"
     CDS             <1..540
                     /locus_tag="IWM52_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP9973829.1"
                     /translation="VQIVTSGPDTEHEQIKMTYLKMISLAKREILIQTPYYIPDGSIH
                     EALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIETYENGFIHAKTMI
                     IDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDSKISTHLTKEMYEN
                     RGIIIKMKEGLARLISPLL"
     gene            877..1734
                     /locus_tag="IWM52_001575"
     CDS             877..1734
                     /locus_tag="IWM52_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAP9973830.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            1849..2769
                     /gene="pstC"
                     /locus_tag="IWM52_001576"
     CDS             1849..2769
                     /gene="pstC"
                     /locus_tag="IWM52_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAP9973831.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            2769..3653
                     /gene="pstA"
                     /locus_tag="IWM52_001577"
     CDS             2769..3653
                     /gene="pstA"
                     /locus_tag="IWM52_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAP9973832.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            3668..4474
                     /gene="pstB"
                     /locus_tag="IWM52_001578"
     CDS             3668..4474
                     /gene="pstB"
                     /locus_tag="IWM52_001578"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP9973833.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            4490..5248
                     /locus_tag="IWM52_001579"
     CDS             4490..5248
                     /locus_tag="IWM52_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP9973834.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGINLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            5261..5938
                     /gene="phoU"
                     /locus_tag="IWM52_001580"
     CDS             5261..5938
                     /gene="phoU"
                     /locus_tag="IWM52_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAP9973835.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            6189..7207
                     /locus_tag="IWM52_001581"
                     /pseudo
     CDS             6189..7207
                     /locus_tag="IWM52_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723340.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
     gene            7204..7971
                     /locus_tag="IWM52_001582"
     CDS             7204..7971
                     /locus_tag="IWM52_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP9973836.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            8071..9639
                     /gene="liaX"
                     /locus_tag="IWM52_001583"
     CDS             8071..9639
                     /gene="liaX"
                     /locus_tag="IWM52_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAP9973837.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEITQVQL
                     STTTGSIYLKDTDK"
     gene            9939..10256
                     /locus_tag="IWM52_001584"
     CDS             9939..10256
                     /locus_tag="IWM52_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAP9973838.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            10256..10606
                     /locus_tag="IWM52_001585"
     CDS             10256..10606
                     /locus_tag="IWM52_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP9973839.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            10773..11711
                     /locus_tag="IWM52_001586"
     CDS             10773..11711
                     /locus_tag="IWM52_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAP9973840.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            11726..12556
                     /locus_tag="IWM52_001587"
     CDS             11726..12556
                     /locus_tag="IWM52_001587"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP9973841.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            12600..13625
                     /locus_tag="IWM52_001588"
     CDS             12600..13625
                     /locus_tag="IWM52_001588"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP9973842.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            13640..14578
                     /gene="galU"
                     /locus_tag="IWM52_001589"
     CDS             13640..14578
                     /gene="galU"
                     /locus_tag="IWM52_001589"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP9973843.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            14703..15143
                     /locus_tag="IWM52_001590"
     CDS             14703..15143
                     /locus_tag="IWM52_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAP9973844.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKATAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            15146..15748
                     /locus_tag="IWM52_001591"
     CDS             15146..15748
                     /locus_tag="IWM52_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP9973845.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDTKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(15807..16913)
                     /locus_tag="IWM52_001592"
     CDS             complement(15807..16913)
                     /locus_tag="IWM52_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP9973846.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            17123..18124
                     /gene="ccpA"
                     /locus_tag="IWM52_001593"
     CDS             17123..18124
                     /gene="ccpA"
                     /locus_tag="IWM52_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAP9973847.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(18461..20806)
                     /locus_tag="IWM52_001594"
     CDS             complement(18461..20806)
                     /locus_tag="IWM52_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP9973848.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSISDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLSKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            21085..21987
                     /locus_tag="IWM52_001595"
     CDS             21085..21987
                     /locus_tag="IWM52_001595"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAP9973849.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTAAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            22200..23975
                     /locus_tag="IWM52_001596"
     CDS             22200..23975
                     /locus_tag="IWM52_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAP9973850.1"
                     /translation="MLNTLKPLINQLTKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHFSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(24041..25627)
                     /locus_tag="IWM52_001597"
     CDS             complement(24041..25627)
                     /locus_tag="IWM52_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAP9973851.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            25878..26102
                     /locus_tag="IWM52_001598"
     CDS             25878..26102
                     /locus_tag="IWM52_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAP9973852.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            26244..>27834
                     /locus_tag="IWM52_001599"
     CDS             26244..>27834
                     /locus_tag="IWM52_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9973853.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIAMIPLPLLAITSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYLKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVKHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLMLDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAH"
BASE COUNT         8913 a         4950 c         5827 g         8144 t
ORIGIN      
        1 gttcaaatcg tgacaagcgg accagatacc gaacatgaac aaatcaaaat gacttatttg
       61 aaaatgattt cgttagccaa acgggagata ttgatccaaa cgccttatta cataccggat
      121 ggatcgattc atgaagcact gaagttggct cttctttcag gcgtaaaagt tcatatccaa
      181 atacctaaca aaccagatca tcttctcgtt tactgggcaa cgtattcttt cgctgcagag
      241 ctgatcgagt atggagcaag gatcgaaaca tatgagaatg gatttattca tgcaaagacg
      301 atgattatcg atggtggcat tgtttcagtt ggctcagcaa atatcgatgt tcgttcattt
      361 cgcttggact ttgaggtcaa tacactggtt tatgatgaac gaatggcttc acgtgtaaga
      421 aaagcttttt ttgaagactc gaaaatttct acgcatctca caaaagaaat gtatgaaaac
      481 cgaggaataa tcatcaagat gaaagaagga ttagcaagat tgatttcgcc gctcctttaa
      541 aacataaaaa ggacatgaga accatcaaca gatggagatc tcgtgtcctt ttctaacggg
      601 gccatttctg agagtacgtc agaaatggag aaagcgtctc gcatgagcga aattaacttt
      661 ggggtatctc ttgttttttg gggaaagcaa gaaataagct aatatatgct attaaaagag
      721 acacatttca aagccagtat agcatagaaa cttttaatga gatagttttt tttacaatta
      781 tgagaattta ttttgttatt taatgatttt gattcgtttt catcaagtaa tgaaaatgat
      841 aaaataaaca tactttagga ttaaaggaga tttctaatga aaaaacttaa attattctta
      901 ccaataatag gattagccat tcttttatca ggctgttcaa agtggattga tcgaggagaa
      961 tccattacgg ctgttggctc ttctgcttta cagcctttag tagaaacagt tgctgaaact
     1021 taccagacag aaaatcctgg aaaattcatc aatgtacaag ggggcggaag cggaactggt
     1081 ttgtctcaag tacagtcagg agcagtcgac atcggaaact ctgatttgtt tgcggaagaa
     1141 aaatcgggga tcgatgcctc agcattagtc gatcatcgag tagcagttgt tgggatcact
     1201 cctattgtga acaaggatat tggcgtaaaa gacatcagta tggaaaacct gaaaaaacta
     1261 tttacaggcg agatcaaaaa ctggagtgaa gtcggtggaa aagatcagcc agtagttata
     1321 ttgaaccgcg cttcaggaag cggaacccgt agcacgtttg aaaaatgggt attggacgga
     1381 gaaacagctg ttcaggcaca agaacaagat tcaagcggaa tggtgcgcca gatcgttgcc
     1441 gacacacccg gtgcaatcag ctatgttgct ttttcttatg tttccgatga tgtagatacg
     1501 ttgaatgtta acggtgtaga accgacagat gaaaatgtga caacgaacaa atggacgatt
     1561 tgggcatatg agcatatgta cacaagagat catccaaaaa aactgacaaa agaattttta
     1621 gactatatct tatctgatga agtacaagat aacatcgttg gagaactagg ttatatccct
     1681 gtttctcata tgaaagtcga acgtgattgg caaggaaata tcgttaaaga gtaatcttta
     1741 cacaaaattt acaaacttag ctaaactttt acatgttatt aaaaccaaat tgatgttcaa
     1801 tttgtacact gagtgagtat gttaaacctg attaaggggg agtcatctgt ggaagatgtg
     1861 aaaaaagtat tattaacaaa atcaaaacgt tcaaaaatgg aacagcgtgg aaaattcatc
     1921 agttttttat gtatcgcgct gatcgtgttg gttgtattat ctatttttta ttttgtagct
     1981 agtaaaggat tggccacctt ttttactgat aagatcaatg tatttgattt tctgtttggt
     2041 acagattgga atccaagcca aatcggagca aatgggaaac caatggtagg ggcattgcct
     2101 atgatcacag gttcgtttat tgtgaccttt ttatctgcta tcgttgccac gccatttgcc
     2161 attggtgcag cagtattcat gacggagatc tctcctaaaa acggaacgaa gatcttacag
     2221 ccggtcatcg agctgcttgt aggtattcct tctgtcgttt atggttttat cggtttatca
     2281 gttatcgtgc cttttgtcag aagtattttt ggcgggacag ggtttggtat cttagctggt
     2341 acatttgtgt tgttcgttat gatcttgccg actgtcacaa cgatgactgt tgatgcatta
     2401 aaggcagtgc cacggcacta tcgggaagct tcattagcgc ttggtgccac aagatggcaa
     2461 acaatctaca aagtcgtgct acgagcagcc gtgcccggga ttttaacagc agtcgttttt
     2521 ggtatggctc gtgcgtttgg tgaagcctta gctatccaaa tggtcatcgg taatgcagca
     2581 ttgatgccaa caggacttac tactcctgct tctacattga cgtccatctt aacaatggga
     2641 atcggaaata caatcatggg aacagttgaa aataatgtgt tatggtcatt agcgctgatc
     2701 ttgctattga tgtcattatt ctttaatatc gtgatccgta tgattgggaa gaaaggagag
     2761 ctgaaataat gaacgcaaaa cgctcagata aaattgctac aggtgtttta tatatagttt
     2821 cggggatcat cgtattgatt ttagcagctt tactacttta tatcttagtt cggggaatac
     2881 cgcatatttc atggtccttc ttgaccgaac cttccaaagc ctaccaagaa ggcggtggaa
     2941 tcgggatcca gctatttaac tcgatttatt tgcttttgat cacgatgatc atcagttttc
     3001 ctatttcttt aggtgcgggt atctatcttt cagaatatgc tggaaaaaac tggctaacag
     3061 atgtgattcg cacgtcgatt gaaatcttga gctcgttgcc ttctgtcgtt gtcggtttat
     3121 tcggtttctt ggttttcgtt attcaattcc agtatggttt ctcgattatt tctggtgcat
     3181 tggctttgac tgtattcaac ttgcctcttt tgactagaac agttgaggag tctttacaag
     3241 cggtccatta cacacaacga gaagcaggat tggccttagg tttgtcacgc tgggaaacag
     3301 tgataaaagt ggtggttcca gaagcgacac cagggatttt gactggtgtg atcctaagtt
     3361 ctggaagaat ttttggtgaa gcagctgcat tgatctatac ggctggacaa agtgcacctg
     3421 cgttagattt tacgaactgg aatccattaa gtgtttcaag cccgatcagc attttccgtc
     3481 aagctgaaac attagctgtc catatttgga agatcaatac agaaggcacg atgcctgatg
     3541 gtgtttctgt ttcggctggc gcttctgccg tgttgatcat tgctgtttta ctgttcaact
     3601 tcggcgctcg tgcgattggc aaacgattat accgaaaaat gacttcagcc taaggagaag
     3661 aagcaaaatg aaaaaatata actggaatga aacaaatgtc atcaccatgc caaaagagaa
     3721 agaagtagca ctatatacgg atgatctaca tgtctggtat ggcgacaatg aagcaatcaa
     3781 aggagtggat ctggaatttg aaaaaaataa gatcactgca ctgattggtc cttccggctg
     3841 cggaaaatct acgtatcttc gttcgttgaa tcgaatgaat gatgggatcg ctaatacgaa
     3901 aatcactggg aaaatcatgt atcaaggcgt agatattaat gcgcctcaag tggatgttta
     3961 tgagatgcgc aaacggattg gcatggtttt tcaacgtcct aatccgttca gcaagtcgat
     4021 ttatgacaat attacatttg ctttgaaaca gcacggcgaa aaaaacaaaa agaaattaga
     4081 tgaaatcgtt gaaacaagct tgaaacaagc tgccttgtgg gaccaagtaa aagacagctt
     4141 ggacaaaagt gccttggctt tatctggcgg gcaacagcaa cgtttatgta ttgctcgtgc
     4201 gatcgcgatg aaacctgata tcttgctgct agacgagccg gcaagtgctt tggaccctat
     4261 ttcgacagga acggttgaag aaacacttgt aaacttgaaa gaagactata cgatcatcat
     4321 cgtgacgcat aacatgcaac aagcagctcg tatcagcgac tatactgcct ttttctatct
     4381 aggaaaagta atggaatatg acaaaactaa caagatattt accagaccaa aaattcaggc
     4441 aaccgaagat tatgtgtccg gtcattttgg ttaagacgga ggcctcaaca tgacaaaaga
     4501 aattatttca tctaaagatc ttcatctgta ttatggaaag aaagaagcac taaaagggat
     4561 caatctaagt ttcaatcaag gggaaatcac tgcgatgatc ggtccttctg gttgcgggaa
     4621 atccacttat ctgcgttctt tgaaccggat gaatgacctg attccgaatg taacgatcac
     4681 cggaagtgtt ttatataaag acaaagacat ctatggtcca aaaacagatg ttgttgaact
     4741 aagaaaagaa atcggcatgg tattccagca gccaaaccca tttccttttt ctatctatga
     4801 aaatgtcatc tatggattga aattaaaagg ggaaaaagat aaaaaagtcc ttgatcaagt
     4861 agttgaagaa agcctgaagg cagcgtctgt ttgggatgat gtgaaagaca aattgcataa
     4921 aagtgcttta tctttatccg gtgggcagca gcagcgtgta tgtatcgcac gtgttttggc
     4981 agttaatccg gaaatcatct tattggatga accaacaagt gcgcttgatc ctgtttctac
     5041 tgggaaaatc gaaagtatgc tgttagaatt aagagagcgg tatacgatga tcatcgtgac
     5101 ccataatatg tctcaagctt cacgtatttc agataagacg gcattctttt tagatggtca
     5161 cttgatcgaa tacaacgata cgaagaagat attcatgaat ccagaaaaac aagagacaga
     5221 agattatatt tcaggtcgat ttggataagg aggaaacgga atgctacgta cacaatttga
     5281 agaagaattg ctgaatctcc ataatcagtt ctacgaaatg gggatgatgg tgagcagcgc
     5341 cgtgcacaag tcagttcgtg catacatcaa acacgataaa atcttagccc aagaagttat
     5401 cgaaaacgat ataaatatca ataacatgga aacacgcttg gagaagaaaa gttttgaaat
     5461 gatcgcattg caacagccag ttacaactga tttgcgtatg atcattacgg tcatgaaagc
     5521 tagctcggat ttagaacgta tggccgatca tgcggtgtct gttgctaaat ccacgattcg
     5581 cgtaaaaggg cagacgagaa taccggaaat cgaaaaagag atctccgata tgtccgatta
     5641 tgtgaaaaaa atggtggaca acgtcctagt agcttatgtc aaaacagacg aaaaagacgc
     5701 acgtacgatc gccaatatgg atcaaagagt caatgactat ttcaatcgaa tctataatgc
     5761 aacaatcaaa aatatgcagg aaaatcctga gacagtcatc agtggaacag actacttgaa
     5821 tgttgccagt tatctagaac ggatcggaga ctatgtgaca aacatctgtg aatggatcgt
     5881 ctacttggct acagggaaaa tcactgagtt aaatacgaac cacaatgaga ttttctaaac
     5941 tgaagctgtg attatgccgt ttgtttagga aaagaggttg tgaaatcagc ggttgtgaaa
     6001 aagctgttct gcatcaagta ataagaacag tcaaacaaaa aatagctcct catatttttc
     6061 gtttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt cgggaaaggg
     6121 tttgtgacaa aacatgtcac aaaccctttt catcatttca tagaaaggat aaagaatgca
     6181 aaaaaaaaat tagcatcatc actatatttt ttatttgtgt gcttacttca tgggttattc
     6241 agcatcaagc tgctttatat gaagtacctg tcatcgagat cgaaaaagta cgaataaaag
     6301 aggatcatca attgattaca ggaagactga tgaacacgga tcaaaaagga gaatcgattt
     6361 atttatcggt tccttatcaa aaaaatcaaa tagaaacagc aaatataaaa gcgagacaaa
     6421 tgtatctagt ccaaatgcat ggagatgaat ggcagctagt agcaaaaaaa aatgatggct
     6481 ggcttttctt cttcacaagt atcttcattg gcgccatgtt gcttattggc ggaaaacagg
     6541 gtctatttgc tttaggaagt ctcatcatca atatcgtttt actttacttt tgttgctcat
     6601 ctttactaaa acagaaggga tttctctatt aaatatatct atcctgttcg tcgttatagg
     6661 gatgattatt agcatattgg ctttaaatgg atggaataga agcagtataa tcaaaatttc
     6721 agctgcagtt acgagtgttt ttttagcatt ttttatttgt cttttgacaa tgaatcattg
     6781 gaaagaccaa ggactgcgct atgaggaact gaattatctg acacgaccgt atcgttctgt
     6841 ttttctttct agcgtactga ttggaacagc tggaggaaca ctcgataccg ttatcacagt
     6901 cattgcgaca ttagaagaat tgactaagcg acaaccagaa ataacagcga aacagttatg
     6961 gctttcagga aggcaagtgg gtcgagatgt gattggctcg atgaccaata tccttttgtt
     7021 cgtctatatc agtgggtcag ttccatcgct tcttttatat ttaggaaatc aatggtcgtt
     7081 caaagaaacg atagaacagc atttatcatt ggaattttta cgcgtcattg ctggcagctt
     7141 aggaatagta ttatcgatcc ctatctcggt cctctttttt ctgactgtaa ggaggctgaa
     7201 acgatgaatg tacttattat acttgttggg attttagcaa tcagcgtgct acttattggc
     7261 ggtacgcaag gtatgtatat tcttttaggc ttattcatca atttagggat ctttttcctg
     7321 ttgttattcg gttatcatca gaagtggccg atactggcat tgtcggtcat aggattttta
     7381 ttgattgctg tggttatctt attttttatc aacggttata atctaaaaat gcgtgcagct
     7441 tttgtgagta tactgatctt tttattttgt ttcctactat tgcttcctat cactgatttt
     7501 ttagcgatcc aaggttttac atcggtagaa ctagaagaat tatctggctt agataagact
     7561 ttagcgattg attttagatt acttacgaga tcgttgcttc tgattagtct tagtggtgct
     7621 gttttagatg ctagtgtagc aatttctagt gggacattcg aagtatatca ggcaaatccg
     7681 caattatctt tttaccaatt gcgccattct agtttcgcta ttgctaaaaa aatattggcg
     7741 tcaacagtaa tgacacttct ttttgcattc atgggaagtt ctttagcgtt gatcctctgg
     7801 tttattgatt tagacattcc ttttcagcaa gtgatcaatg aaaaatcatt tgttctagaa
     7861 tatacgatgg ctattcttac cacattgtct gcactacttg ttcttccttt aacctgcgtg
     7921 actacagcat atctctttac aaaaaagaaa gaacaactca agatggagta aaagttacaa
     7981 cttaagtcct atgacatttt tactgaatca gtgcataata tggatgtaag gaaaagcttg
     8041 caaacaattt tgcttaagga ggcaatttcc atgaaagaaa gagaacgtat tttagattta
     8101 gtgaaaaaag gaatcctttc gactgaagaa ggcttagatt tattagaaag catggcaact
     8161 gaaaaagacg aaaagcaaat aaaaaaagaa gcagacaagg taactgctag tcataaagaa
     8221 aaagatcagg caagtcaatt gatcgataaa cttgaaaacg gcgaagaaga aatttctgag
     8281 ccagttgatc caaaggaaag agaaagacaa gatcaagaaa acttagaaaa gatcttagat
     8341 gaactagcaa ctgaagcgaa taaaacttcg gcgcgtctag atgaagtaaa tgctcaattc
     8401 gctgacaata aggcagcacg caatgagaaa caggaagcat tgatgcagct gaatacaaaa
     8461 gaagaattag gcgagttgac agaagaagaa ttagcacagc gtcagacgtt agaagcagaa
     8521 atcaaagaat tagaagctag tggcgataca ctgttagaag aacaattgaa attagaagca
     8581 gaattaaaag atatccgtaa gaatcaatgg tctgagaaaa aagaaacgtt tactgataag
     8641 tttgaattgc cagatgattg gaaagatcaa gcaacagata cactgaatca agtaggcgaa
     8701 aaaatgtcag aagctggatc acagttaggc aaattcttga agaaaacatt ccaaactgtt
     8761 tcagaaacag tgaatgacaa catggaatgg aaagatgtca gcttacgtgt ccctgggatt
     8821 gcgacaacga aatttgagca tgaattttac tatgaagctc ctgcagcaag tatccttgat
     8881 atcaaggcag caaacggaaa cgtgacgtta aaaacgtggg acagcaacga tgtgaaagtt
     8941 gaagctaaga tcaaacttta cggaaaaatg ggtgctgaac catttgaagc attctctgaa
     9001 cggagccaga ttgaggttaa tgaagatcat atctccttcc aaattccaaa taaacgtgtg
     9061 cgtgcagacc ttgttttcta cttacctaaa cgagtatacg accacgcagc tattaaactt
     9121 ctaaatggca acatcatgat tgaaacattg gaagctaaag atatttatac aaaatctacg
     9181 aatggtaaca tcatcgtgga ccaattaact gcaacgatgc tggaagtaga aggtgtaaac
     9241 ggcaatatcg atattcgaaa cgggaacatc ttagattcta ttatcgaaac agtaaatggt
     9301 acagtgacat ttggtgcgac agcagaaaac ctttctgtgt ctttggtaaa tggtgatgta
     9361 cgtcttacga ttaaagaaga taatttgaaa aaagtagaag caagttcagt gaacggaaat
     9421 gtcaaagtcg ctttaccaga tggcatcggt ttggaaggac atgctaaaac aagtcttgga
     9481 agtatcaaca gccgtctgtc caattacgaa gtagttcgtg agaaaaaaga acggacaaac
     9541 caaatgttgc aattccgtcg tgtttcagat ggtgaaatca cgcaagtgca actttcaacg
     9601 acaacaggaa gtatctattt aaaagataca gataaataaa gaggttgtga aagagatgtt
     9661 cagccttgag caataagaag cgattttgta aaatagcttc ccctatttta caaaattgtg
     9721 ccttattgcc gataggctat ctcttgaaca ccgtttacca agagattatg aacctgccaa
     9781 ttatcgccga gtcacaagga ataatttcag aaaaagctcg tcacgtttta tgaatatggt
     9841 aatttgtgac cgagaatttt gcagaaagac accgattaag aagaaggttg tcacaacgat
     9901 tttcttcatt taaaatagta gaacagaggt gagacatgat gaaaaaaaag ttgacaaaat
     9961 ccaataaaaa tgttgttttg acagggactt tagcaggaat aggagaatac atcgggattg
    10021 atccgactgt tatccgtgtg ctttatgtat tcctgagctt agttgctttt ggaagtccaa
    10081 tcattttata cattttatta gctttgatta tcccatctgc gcgttctgca cgaacaaact
    10141 atggacataa caatgcatat tatgaaaaaa acaattatcg tgaacaaact cgtgaacaaa
    10201 caaaaaaacg caaagaagcg gaaaaaatcg atgatgacga atggagtgac ttttaatgtc
    10261 gtactttcaa cgtattttag ttaatacgtt aacattcata tcattggctg ttctttttcc
    10321 tgcgatggtc catgtgagca gtatttggac ggccgttctt gccagcttcg tattgtctgt
    10381 gttgaatatg tttgtcaaac cgattttgac gatactgtcc ctgcccttta cattagtcac
    10441 attgggattg tttacgtttg ttatcaatgc agcgattctt aaaatgacct ccttttttgt
    10501 gggagcagaa cattttagtt tttcttcatt cggtgcagcg ttgttagtag ctatcttgat
    10561 gtcgcttgtc aatgtaattg tcagcgaaca taacagtaga aaataaaaga aagctgtgcc
    10621 gaactatcgg cgcagttttt ttatttagaa tcaggaatgc gttttctatt acattggtaa
    10681 ttaaataaaa acgttttttt taattggtag tacttttggc tagacaatga taaaatgata
    10741 gagaacactc taatagaaat gaggtagcat gaatgtcaga agttgtaaaa gttagccaat
    10801 tagtgaaaaa attatctttg gaaatcgtga caggcgacga gaaaagctta gaccgtgtca
    10861 tcgtaactgg ggacatctca cgcccaggac tagagttgac tggttatttt aattattatt
    10921 cccataatcg tttacagcta tttggcagta aagaaatcag ttttgcggaa cggatgatcc
    10981 cagaagagcg tttgatcatc atgagaagat tatgtagtga agatatgccc gcatttatta
    11041 tttcaagagg attaccggct ccagaagaaa tgatccaggc agcaaatgaa aatgggatag
    11101 ctgtactgcg ttctcccatc tcaacttctc gtttattagg agaattatcc agttatttag
    11161 atggtcgcct tgcccctcga acaagtgtcc acggtgtttt agtggatgtt tacggattag
    11221 gtgtactgat tcaaggtgac agtgggatag gaaaaagtga aacagcgttg gagttgatca
    11281 aacgcggtca tcgattgatc gcagacgatc gagtagatgt ttatcagcaa gatgaattaa
    11341 cggtgatcgg agaaccgcct aaaatcctgg aacatctaat tgaaatccga ggaatcggga
    11401 tcattgatgt aatgaatctt tttggtgcga gtgcggtacg tggctctatg caagtccagt
    11461 tggctgttta tctggaagca tgggcaaaag ataagaaata cgaccgtctg ggctctgaag
    11521 acacgaccgt tgaaatcgcg gaagtgggca ttccacaaat aaaaatccct gtcaaaacag
    11581 gacgaaacgt tgcaatcatc attgaagtag ccgcgatgaa tttccgtgca cgtacaatgg
    11641 gctttgacgc aacgaaaaca tttgaagaaa gattaagtcg cttgattgaa gaaaactcag
    11701 gcaacgacta gcaaggagtg acaatatgtt agcacaaatc aatcgagttg cttttgagct
    11761 tgtagggatt cctatctact ggtatgcatt gatcatcgta tcaggtatca ttatcgccat
    11821 gtggcttagc agcagagagg ctgttcgggt aggattaaaa gcagatgatg tgacagattt
    11881 tatgttgtgg ggattgccta tctccattat tggcgctcgc ttatattatg ttttatttga
    11941 tcttccccag tatatagctg atccaatcca aatattcaat atccgttcag gaggcttagc
    12001 tatttatggc ggactgattg caggcgcgat cacgctgtat ttttttacta aatatcattt
    12061 tatttctttg tggacttttt tagatatcgc tgcaccaagt gtattattag cacaggcaat
    12121 cggtcgttgg ggaaatttca tgaaccacga agcatatgga ccagacacga cgcgaagctt
    12181 cttagaaagt ctgcatttac cgcaattcat cattgataat atgtatatcg aagggatcta
    12241 tcgtcaacca acatttcttt atgaatcact ttggagtttg attggattta ttttgctttt
    12301 gtttttacga aagaaaccac accttttgaa aaaaggcgaa gtccttttat gttatctaat
    12361 gtggtatagt ttcgggcgtt tctttattga aggattacgt acagacagtc tttacttttt
    12421 aggtgtgatc cgtatttctc aattattatc tgctgtgtta tttgtcggta cattgattct
    12481 atttatttgg cgcagaaaaa aaggaaattt accggattat gaccgctcat taggaaaaaa
    12541 tcaacaaatt atctgatttt tagagcgtta aaaatgaaaa aaatttcagg agtggtaaaa
    12601 tgaattatag agtagcggtt ttaggaccag gctcttgggg tactgctttg gcacaagtcc
    12661 tcgccgaaaa cggacatgac gttcggattt ggggacatcg tgcttcacaa gtagaagaaa
    12721 tcaacaccca acacacgaat caacgttatt taccagatca ttatctaccc gattccatcc
    12781 aagcattcac caatatggaa gatgcagtaa aagacgcaga tgcggtgcta ttcgtcattc
    12841 caacgaaagc aatacgttct gttgcaaaag aactagtgcc taaattgaat acaaagccag
    12901 tgatcatcca tgcaagtaaa ggattagaac agggaacaca taaaaggatc tctgacatct
    12961 tgttagagga aatcccagca gaaaaaagaa aagctgttgt tgttctttct ggaccaagcc
    13021 atgcagaaga agtagcgaaa catgacatca ctacgatcac agctgcaagt acagatgcaa
    13081 aagcagcaaa atatgtacaa gagctgttca tgaataatta ttttcggatc tataccaatc
    13141 ctgatgtgat cggtgtagag atgggggcag ctttgaaaaa tattattgct attggtgctg
    13201 gcgctattca cggcttaggg tttggcgata atgcaaaggc agcaatcatg acgcgcggat
    13261 tagctgagat cagccgttta ggtgtagcaa tgggtgccaa cccgctgacc tttatcggat
    13321 taagcggagt aggcgatcta gtcgttactt gcacaagtgt tcattctagg aactggcgcg
    13381 caggcaactt gttaggacaa gggcagcccc tagaagaggt gctagccaat atgggaatgg
    13441 tcgttgaagg tgtgaatact acccaagcag cgatggaact agcccaaata atggaaatcg
    13501 aaatgcccat cactcaaaca atctatgaag tgctgtatga acaaaaagaa atcaaaacag
    13561 ccgcaaaaga aatcatgtta cgtgatggga aaaaagaaaa tgaattctca ttaacaattt
    13621 tttgaaggag cgaatgtata tgaaagtcaa aaaagcagtt attccagcag caggattagg
    13681 aacaagattt ttaccagcaa caaaagctat ggcaaaggaa atgttgccaa ttgttgataa
    13741 accaacaatc cagttcatcg ttgaagaagc gttgaaatca ggaatcgaag acatcttgat
    13801 cgttacagga aaagctaaac gaccaatcga agatcacttt gattcaaata ttgaattaga
    13861 aacaaaccta agtgaaaaag gaaagaccga attattgaaa ttagtagaag aaacgacaga
    13921 tgtcaacttg catttcatcc gtcaatctca tccaaagggt ttaggacatg ccgttcttca
    13981 agcaagagcc tttattggaa atgaaccgtt tgtcgttatg ctaggcgatg atatcatgga
    14041 agatgaagta ccattgacac agcaattaat cgatgattac gaacgtactc atgcttctac
    14101 gatcgccgtt atgcaagttc ctcatgatga aacttcaaaa tacggaatta tcaatccagg
    14161 agaagtattg gaagatgggt tatacaatgt caaaaacttt gttgaaaaac cagatccaag
    14221 caaagcacca agtgatttag ctattattgg acgttactta ttgactccag aaatttttga
    14281 tgtattgtcc gagcaacaac caggagccgg aaatgaaatc caactaactg acgcaatcga
    14341 tacattgaat aaaacacaac gcgtatttgc ccgtaaattc acaggtaaac gttacgatgt
    14401 cggtgacaaa tttggtttta tgaaaacaag tattgaatat ggtctaacac atccagaagt
    14461 aggtgcacct ttacgtgagt atatcctgtc tttaggtaaa acattagaag cagaaggaag
    14521 caagatcgat cataatccaa aaggaataac agaaatcaaa gaagaaaaga aaaaataacc
    14581 cttttttcta acgagcttca gctgctaatt gaataaaaag tagttgaagc tcgtttttat
    14641 ttgtttaaaa aacacgaaaa tgctatcctt atagaaaaga gagacaagaa gggagtttga
    14701 tcatgtcagg aggagaaatt gcaggattga ttgcagcaat cgcctttgcg atacttgtcg
    14761 tttttatcgt tcgtgtgttg ttgcaagttt ctaaaacttt agaaaaagta gatcagactg
    14821 tagcagaagc aaatacgacg atcgaaatcg tcacaaaaga cgtagatctt ttgtctagac
    14881 aagtagaagg cttgctggtc aaaagtaatg aactgctctt ggatatcaat ggaaaagtcg
    14941 caacgatcga tccattgttc acagcagttg ctgatttaag tgaaagtgta tcagaactta
    15001 atcattctag taaaaacatc gcgacgaaag tcggcggaat cggtaaagcg acagctaaag
    15061 cgacggttgc cggaaaagtc ggtgggacag cgatgaaatt ttttaaaaac aaaaaacata
    15121 ctgaaacgac aggaggaaaa taaaaatggc taaaaaaggt ggatttttat taggtgcagt
    15181 agtcggagga acagccgcag caatcgccgc gttattgtta gcaccaaaat ctggtaaaga
    15241 acttcgggat gaattagctg cccaagcgga tgatttgatg gatatggctt cagattacac
    15301 agatatggcg aaagaaaaat cgactgagtt aacaggtatt gcaaaagaaa aagcgcaaga
    15361 tctttctcaa caagcaggtg atctagcagg tagtgtcaaa caaaaagtaa gtgaatcagc
    15421 agatgaagca ggaagtgaat ctgacgatat cttgttaaat cttaaagaac aatcagctga
    15481 cttatctaat cgtttcaaaa aaacggcaga agatatgaaa aaacctgcag atgaattagg
    15541 aagtattgct aaagatactt cagaagatat catgattgat gtaaaagacg ctgctggtaa
    15601 agcaggagat actgtcaatg aaggaatgtc agaagcaaaa ccagttgcag atgacacaaa
    15661 agaaatgatg aaaatggcaa aagaagatgc gaaagaagct aaaaaagaaa taaaagatac
    15721 aatgacagat tcatctacaa atcactaaaa tcacaaattt atagaataaa aaggtgggag
    15781 cggactagct ctcacctttt ttactcttat ttttctaaaa caagtaattc ttttggtgta
    15841 gtcgtgaatg gcacacagcc atcgcttgtc acatatacac agtcttcgat acgtacccct
    15901 actttttcag gaatatagat tcctggttca agagagaagc acatcccttc ttcgattact
    15961 aagtcatttc cttctacaag ggaagggaat tcgtggacgg ttgttccgat cccgtgacct
    16021 aaacggtgat tgaaatattc gccgtagcca tagctgttga tcacgtttcg tgcgatttga
    16081 tcaagttcac ctgctgtcac acctggacgg acggcttctt gtgcggcaag ctgtgcttca
    16141 agagtgatgt tgtatatttt ttcttggaag tcggttggtt tttgataagc aattgtacga
    16201 gtagcatcac tgcaatagcc attccaaaca acgccaagat cgaataacac aagttcattc
    16261 ggtgaaatct tcgtgtttcc tggtgtacca tgagggcttg cagcatttgc tcctgcaagg
    16321 actaatgtgt cgaaagacat ttgtgaaaca ccttgttttt tcaattggta ttcgatttct
    16381 gcaatgatcg cttgttcagt cacgccttct ttcactgctt taaagccaat ctcaaatgca
    16441 acatcggccc atttaccagc ttcgatcagt ttgtcttttt cctctagagt tttgatcagt
    16501 tgtagacgtt ggatgatgga agttagatca gtgtaagaat ccgttcccgt caagatatca
    16561 gagatagcat cgaagcgctc gacagtcaag gcgttctttt caatccctac attgccaggc
    16621 tgaccatagc gagcacgaag ttcttttccg atgatctccc aagggttttc gctgtctaaa
    16681 tagccacgga catcatacgc ccaactactg ttttgagcat cttcaacttc caaagcagga
    16741 gtgaaaagga aaggatcttg ttcaagaggg ataaaaagag ctagtacacg ctcatgagga
    16801 tcactttcgt atccggaaaa gtaagcgatg tggccagggt cgctgatata agccaattcg
    16861 acttggtgtt gtgccatcca ttgacgtagt tcattgattt ttacgttatt catttggcaa
    16921 cctactttct aaattaattt tgtattcaat aattgtacca ctgatttatt aaaagaccaa
    16981 cgttctaaaa actgaaaaaa gtaaaaattt tcatgaaaac agagataaaa cggttgcaat
    17041 tcttgagatt atttgatatt ctattttaga aatgtaagac gtattttcag aaaacatata
    17101 aaatatgaat aggagaacac aaatggaaaa gcaaacaatc actatctatg atgtagcgag
    17161 agaggcaaat gtttcgatgg ctactgtttc acgtgtcgta aatggcaatc caaacgtcaa
    17221 accagcaaca agaaaaaaag tcttggaagt tatcgatcgc ttggattatc gtccaaacgc
    17281 agttgcgcgt ggcttggcaa gtaagaaaac aacaacagtt ggtgtaatca tccctgatgt
    17341 cagcaatatg ttctttgctt ctttagcacg tggtatcgat gatgtggcaa caatgtacaa
    17401 atacaatatt atcttagcaa attcagatgg caacgaccaa aaagaagtca atgtattgaa
    17461 taacttactg gctaagcaag tggacggcgt gatcttcatg ggtcaccata tcacagacga
    17521 aatccgtgga gaattttcac gttcaaaaac tcctgttgta ttagctggct ctattgatcc
    17581 agatgaacaa gtaggtagcg taaacattga ttatgtatcc gcaacaaaag atgcagtaaa
    17641 caaattggca aaaagcggca ataaaaaaat cgcttttgtc agtggtgcgt tgatcgatcc
    17701 catcaatgga caaaatcgtc tgaaaggcta taaagaagca ttaagcgaaa ataacctacc
    17761 atatagcgaa ggattgatct ttgaagcaca atacaacttc aaagatggtc tatctctagt
    17821 cgatcggatc cataatagcg gtgcaacagc tgtctatgtt tcagatgatg aattagccat
    17881 cggtattttg gatggtttat tagaccgtgg cgtgaaagta ccggaagaat tcgaaatcat
    17941 cacaagtaac aattcactac tgacagaagt agctcgtcct cgtttaacaa gtatcacaca
    18001 accattatac gatttagggg cggtatctat gcgcctattg acgaaaatga tgaataaaga
    18061 agaagtcgaa gaaaaaacga ttatcttgcc ttatggaatc gaagaaaaag gatctacaaa
    18121 ataataaaag gacaaccatt cagaggttgt gtgcctgccg tttaactctg cgttacaagg
    18181 agcaatttca aaaaacagca tctcatgttt gatgaatatt gtgactcgtg attcggagtt
    18241 ggcaggagaa caccgtttat ttgggaaaag gggttatgac aaaattttgt tataatcctt
    18301 tttttgtgtc ttaaaaaaga gagaaagtgg ttgaaattat atttatcgta tataaaacaa
    18361 tccgtgtata gatgctgtat tttgttcacc atcatcaaaa aaataaggga atgtgacaat
    18421 actattgtca cattccctta ttactgtgta ttctattaaa tcaatcatct ttctcatctg
    18481 attctttggt tgcttgattc gctcttgcat aagctgaagc ggtgttccag taatctgtat
    18541 agttttcatc ggtaccgcca acgccaaact taaaggcgct tttcttcggt ccatttttcg
    18601 cccatagcga ttcaactgtt tttcctggtg tttggatgga ttttttattg acagtaactt
    18661 ttccaggtgt ttgaccagta aatccagata cttttttctt catcacatca gatgataatt
    18721 cgaatttctc atcagtgcca aaaatgtcag gttttgcttg gtaagcttga ttgatcagat
    18781 tggcaagata attagccgaa cgtatccttg ctttgctgtc cattccagta ttgtcatcgt
    18841 gccctgccca gctactgatg gtaatagacg gagtagagac aattagccat gaatctcggt
    18901 attcattggt agaaccagtt tttccgaccc aatctgcttt tcctagattt ccgtttaagc
    18961 ttgagatggc atctttgaat ggtgtagtga tttttgcatt gatcacactg cgcatcatgt
    19021 cattcatgat cgatgccgtt gcttccgaat agatccggac agagttgctt tcgtgtttat
    19081 aaattacatt tcctgcgtta tctgtgatgg aatcgatgat atagccttct tcatagacgc
    19141 cgccgtttgc taaagcctga aatccgttgg tctgttgaag cgtcgtgaca tctactgtac
    19201 ctaaaggagc agattcaacg ccccaattgt catttgccgg gtagttcatc tttgacaagt
    19261 agtgctcgta agcatattgt ttcgatccac cggaattcaa cacatcttga tataagtgat
    19321 aagccgggat attgttggac cattctagtg actcacggac ggtttggaag gtgttcgatc
    19381 cttcgttagt agcattgacg attttctcgc cagcattttc tccttcttgc caagttgttg
    19441 cataatctga tacacgggat tctgatccga taagccccat gtcgattgca ggtccgtaaa
    19501 caagaactgg tttgattgat gagccagctt gacgttctgt atcgaaggca tggttatttt
    19561 gattctgact gtaattccgt ccgccaacga atccgtagat gcggccagtt ctgttatcca
    19621 tcaatacatt tccggtttct acttgactgc cagcaccatc atctaacatg tatccataat
    19681 tttcaacgcc attttgcaat gcagcataga catctttatc gattgtcgaa tggattgtat
    19741 atcctttatt ttggatcgtt tgctgtgcct tttgataata agcgtcactg atcgatgaat
    19801 cagacatatc agcgttgtct ttttcagcca attgttgtgc aatgattctt gttgcttcat
    19861 tcatcacagt ataatacaag aattctctgt cagtctgttc tgcgatttgt tgtggtagaa
    19921 agtcttttgt aaggtcatag gcttttgctt cttcgtattg ttctttcgta atttggcctt
    19981 cacgatacat actgaataaa acaaaatctt ttctggcaag gccggcagaa agatcttctt
    20041 ttagcgcacc tgtgttcgtg tatggactat acgtgatcgg gctttggggt aatccagcga
    20101 tataagcagc ttgcggcaga gtaacatctt ttgcatctac accaaagatt cctcttgcag
    20161 cttcctggac tccagcaatg ttttgtccct tattattacg accaaatggt gagacattca
    20221 aatacgtagc aatgatctca tcttttgaaa agtatttttc tacttgtgca gaaagaatga
    20281 tctcgtttgc tttacgtttg aatgttgttt catctgtaag gatctgctgt ttgactaatt
    20341 gctgtgtcaa agttgatcca ccagaagagc caataccagt agcttcagat actaatgctc
    20401 gtaaaacagc tttaggaacg taaccttggt gtttatcgaa gtattcatcc tctgtgctga
    20461 taattgcagt tttcaataaa ggtgaaattt tatcagatga aatagtcgtg cgcatcagat
    20521 ctgtctggat ttttgatatc tctgtattat ctgcataaac aatctttgac gtttctgtaa
    20581 tgtttcctag ttctgtctgc agtgcttttt tagtcggcaa ttgtgtgtct tcgacgagat
    20641 aggcaaaata gccagcgcct attccaatac ctagagctgc gaacatcaaa agaagaacag
    20701 taataaaaac agtcaatgat tgaaaaactc taaaaataat cttaggaaca aaccattttt
    20761 ctttttttct tctattctgt ttttttcgag tatttttgcg tggcaaaagg gtcacctcaa
    20821 ctaatggttt tatttcgcta attatagcaa ataatagaac aaaaaaacag acaaggctgt
    20881 ttttggcagg aatttctttt ttaaaggagt taaaacgtga gaaaatgatc ttttttataa
    20941 aagagttctt aagcttttct ttagagaaaa tttcagttac tatggagaac agggcaaaga
    21001 aaaatcaatc attttctttg tatatcatct taccaatata taggaattgt tagtaacaat
    21061 tgcctttcaa ggaggaatcc atccatgtta ttaggctcac acgtcagcat gagcggaaag
    21121 aaaatgctgt taggagcagc agaagaagca gccagttatg gatcttcgac ttttatgatc
    21181 tatacaggcg cccctcaaaa cactagaaga aaagccatcg aagagatgaa tattgatgcc
    21241 ggaaaagcgt acatggcaga aaacggtcta acaaatatcg tcgttcatgc cccttatatc
    21301 attaatttgg ggaatacgat caaaccagaa atgtttcctt ttgccgtcca atttctgaga
    21361 gaagagattg ttcgtgcgga agctttaggc gctactcaaa ttacacttca cccaggtgct
    21421 catgtagggg caggaacagc agccggaatt gctagaatta ttgaaggctt gaatgaagtt
    21481 ttgacaaaag aacagacggc acaaatcgct ttggagacga tggcaggtaa aggtacggaa
    21541 atcggtcgaa catttgagga attagcgcaa atcatcgatg gagtaaaatt gaatgaaaaa
    21601 ttatccgtta cttttgacac ctgtcatacg aatgatgccg gttataatat taaagaagac
    21661 ttttcaggtg taatggaaga gtttgataag atcatcgggt tagatcggct taaagtactc
    21721 catatcaatg attctaaaaa tccgcaaggt tcgcataaag accggcacgc caatattggt
    21781 tttggaacaa tcggattcga cacgttgaat aagatcgtcc atgatccaat gtttgacaat
    21841 atttcgaaaa tcttggaaac tccctatgtc ggcaaagata aaaagaaagc ttacgcacca
    21901 tatggaaaag aaatcgccat gttcaaagcg caaacattcg atccagaagt gtttcctgag
    21961 ttattagcag aagaagtgat actctaagat tttctatttg acagcagcaa aaaaggacca
    22021 ggcacattgc cttgtccttt tttgtttaag aaacaaagga gtactttttg cagtcttttt
    22081 catcatgcat cttctccccc ctctttttaa atttagacaa acagggtata ctggtagaaa
    22141 agtaaaaaaa cgaaaagttt cacaagcaag tggcaaaacg tgattggaag gacgaaaaga
    22201 tgctaaacac tttaaaaccc ttgatcaacc aactgactaa gaaacataca tactttattc
    22261 ttggcatagc cctattattt acaattattc gtttgtattt atatagtaaa gcaatctatg
    22321 gaacagacat gtcggcaacc tatgatgatc agttgctgat tcgctatgct caaaatattt
    22381 tggacggtaa atggctaggg gagtatacga ctacaacact ttccaaagga atctcctaca
    22441 gcttattttt agtacttgca cataaactgc atttttctta tgcagtttta ttcggcatgt
    22501 tcagtatttt tgccagtata ttggttgcac tcgcgtttaa accgatcgtg aagaacaagt
    22561 atcttctttt aagtatctat ttttattttt tgttttctcc gattagtttt acacatgaat
    22621 attccactcg aacgtatcgt aatgccctcg tgattccggc tgttgagatc attatcgctt
    22681 gtttattagc tatttattac cgcaaatacg gttctagcag aaaattgctg ccatggtttt
    22741 tagggctttc tgtcgctttt cctttttttt ggttcattcg cgaagattct atctggcttc
    22801 ttccttttac tgtagtagct ttgggtatca gtttcgtcca gatcttgcta gggcaagact
    22861 tttctttcaa agaagtaaag aagttaaaaa aacgactctt tcttctctcg aaacgggaat
    22921 ggctgaaact ttcattgttt ttacttccta tagttggctt ttccatcgtc tatcagtgga
    22981 tcgaatataa aaatgagaca acatacggta ttgcaaccat caatgaccgt tcgagtggag
    23041 aatttggacg tttgatgaaa caactgatcc gaatagatga tgaaacgaac ttgaataaag
    23101 aaaacagtga gatatgggtc tcacatgctg cactaaataa agcattagcc gcatctccta
    23161 cattccatac tgtttctacc cgtattgacg aattatatac gactcacgcg tggactcaag
    23221 gtggaaaagt aaaagaatta cccggagata tcatcttttg ggcattgcga gatgtagtaa
    23281 gcgaatctgg ttattaccaa aacaatgcaa aagaaaccaa tgatttttgg aaaaaagtca
    23341 accaagaatt agaagaagcc tatcacaagg gaaccatcaa aaagaaaaaa gagttctatc
    23401 tgatgggatc aggagatgga aagacgatga gtgatctgcc aatcgtattc gatttccttc
    23461 aatcaggata cgcttatgca tggcgatata aagatttcca tcaaggagga aattatagtt
    23521 tcggaacgca agaacaagtc aaacaggctc aagaattatt aaaccttccg ctaatggaga
    23581 attggatcga tagtaatgaa ccgagaacac ctaacttcca attaacaaaa tcagcaaaag
    23641 tcgcgaatgt catcataaaa atttaccaag ttctggctat tcccgtgctg attgcttcaa
    23701 tagcagggat attacttatg atactaggaa ccgtatttgc aaaagctgat cgtgcctatt
    23761 atagttcctc tttgattgtg gtcaccggat taatactgac acaattggtt tttcttttcg
    23821 gtgtctcatg gttttgttct tatgcgccag aaaaaaagga ctatttttta agtgtctaca
    23881 ctggtgcagg cgtgccatta acgcaattca tcgtagtaac aagcatgcta gttttcagta
    23941 aattaaatct gaaaaaagct ttatcttcta aataaaaaga cccttggtag ctagattttt
    24001 aacctaacta tcaagggtct ttgtgtggaa aaatcagttt ttattcgatt tcttctaccg
    24061 tttcctgcaa aagctgttca tattttttgt ttcctttata taaatcacca atcgtccatg
    24121 ccaacaggag aaggacaaga acaatgatta cataagcgat gtgatcagcc aatgccagtt
    24181 gagcggaagt ggcttgctcg ccgaagaaac tttcgatctg tcctggtaat cctcgtaagt
    24241 tcaagtaagt tagactaatc acagagaacc agccaagaag ttttacgatg aatgaatttt
    24301 taaatcggct acccatctcc gcttggctgt tggtcatcat cagtaaagga atcatcgaaa
    24361 atggcaacgc aaaagccaag aacacctggg attcattcat taagttattc aaagcggtat
    24421 gttcttcgat cgttcctttt gtacttgtaa acaaaacaca aatcaataca ggaatcacag
    24481 aaagcaatcg tgtgaccagt cttcttagcc aaatcgggat acgcatatgg atgaatcctt
    24541 ccatgatgac ttgaccggtc aacgtaccag taatagtaga gttctggcca gaagcaagaa
    24601 gtgctacagc aaataaagtg gacaagatac cagattttgc tacagcaatc aaaataccat
    24661 tgctcatagc tgatggatca gataaagctt catacagacc aaaaaaggaa ggatcttgga
    24721 cagaaccggt tttgaagaca gcgacaccca tgatcaatag cagtgcattg actagaaaag
    24781 ccattgacaa ctggatatta gaatcccaag tagaaaaacg tacagcttgt gctacatctt
    24841 cttcgttttg gtgatcgatc ttccgagttt gtgaaactgc tgaatgaaga tataagttat
    24901 gcggcatgac tgttgcacca ataatcccta aagctccagt tagcggagtt tcgccaccaa
    24961 tgacaggtga gtctgagaat gtatggctgt tagggataaa gccagcaaat aaactgtgcc
    25021 aatctgggtt agacaagcca acttgataga caaaaacgaa taaaatgacg aaaattaagc
    25081 agacaaccag tgcttcgatc ttacgaaacc cgacttttgt cagtagtaag agaacgaaaa
    25141 catctaatac agtaataaag acggcaatca ccaatggaat atcaaaaagt aagtagaggg
    25201 cgattgctgc accaattact tctgcaatat ctgttgccat aatggctaat tcagtcaaga
    25261 tccataaaat gacacctaat gtcttacttg tgcgtgctcg gattgcctgt gccaaatcca
    25321 tttggctcac gattcctaat tttgctgcca tatactgtaa aagcatagca ataagactag
    25381 acatcaaaat aactgacatc aataaatatt ggaagttttg tcctccggtg atcgaagtag
    25441 accagttacc cggatccata tacccaactg ctactaacgc accaggtcca gagtaagcaa
    25501 gaagtgtttt aaaaaaactt ttcccctttg gtacatcaac tgttccattt acttcagcta
    25561 aagataaacc atttgtatgt tcaataagtc gatgcttatg aaattgttta ttggattgat
    25621 tattcaagaa aaaaccacct ttcctattta tctacgatta cattctacac ctcaaaaaga
    25681 aaaaattaaa gaaaaagatt cggtatgcct aatttatttt cgtggcgtgt cttgctagat
    25741 gacgaaagaa attttgttct cctctaaaaa aaccgctatt ttttgcatga aactaaagaa
    25801 tacgcctttc ttttttcaat aaactttagt aaaataagtc attgagagca attcggaaat
    25861 agaaaggaga gatttatatg ttatcaacag gtattgtcgt acttattgct attattgctt
    25921 tgctaatcgg cgcagtaggt ggctttttcc tagcgcgtaa atatatgaaa gactatttag
    25981 aaaaaaatcc tcccgttaat gaggacatgt tgcgatcaat gatgatgtca atggggcaaa
    26041 aaccatctga gaagaaaatt cgccagatga tgcagcaaat gaagaaccaa gggaaaaaat
    26101 aggttctgac ccaacgactt tttacgggct gattttttca gcccgttttt tatttcgtaa
    26161 tttgttagac tattcagatt attcagcaaa aaagacaagc acagacaatc ctaggctctt
    26221 gataaagaca aaggaggagt aaaatgtcga tttttagaaa attaggctgg ttttttcgtc
    26281 aggaaaagaa acattattta attggagttt tctcgctatt cgcagtggcc ttggtacagt
    26341 tagttcctcc aaaagtgatt gggatcatca ttgatgagat tgccgataaa aatttatcaa
    26401 taaagatcat tttcgggtgg atcttcctat tgattatcgc tgctttttta caatatcttt
    26461 ttcgttatat ctggcggacg aatatttggg gaagtgcagc tcggttggag aaagaacttc
    26521 ggcagcagtt attcaaacat tttacagaaa tggacaatct cttttaccaa aaatatcgga
    26581 ctggggattt gatggctcat gcaacgaatg atttgaatgc gatccagaat gtagcaggag
    26641 ctggtatctt gacctttgca gattcagtga ttacgggcgg aacaacgatc attgcgatga
    26701 ttttgtttat tgattggcgc ttaacactga ttgcgatgat tcctctgcct ttgctggcca
    26761 tcacctcacg agtgctggga tcgaaactcc atgatgcttt tcgtgattcg caagaagctt
    26821 tttctagtat caatgataag acacaagaaa gtatcactgg gatcaaagtc attaaaacat
    26881 ttggacaaga aaaagaagat ctagcggatt ttgaagaaaa gattgaagat gcaattatta
    26941 aaaacaaacg agtcaatttt ttagactcct tatttgatcc attcattacc ttgattatcg
    27001 gtctgtctta tgttgcaaca attattttag gtggaaagta tgtcttggaa ggcacgatct
    27061 cgatcgggca gatggtctca tttattagtt atatcggtat gcttgtttgg ccgatgtttg
    27121 cgattggtcg attgttcaac gtattggaaa gaggaaatgc ttcttatgat cgcgtcaatg
    27181 aacttttgca tgaaaaaacg catatcgtgg aacaactggg tgcagtgcag accccggcaa
    27241 aaggcgagct gaatgtagct atttcctctt ttaattatct gaaagaagaa tcaccagcat
    27301 tagaacaaat caagtttgca atccaagaag gagaaacatt aggtattgtt gggaaaactg
    27361 gtgcgggaaa aacgacgatc atcaaattac tgatgcgtga atacgatgat tatcaaggga
    27421 aaatccaatt tggcggacgt tcgatcaaag aatattcatt gaatgcttta ttaggatcaa
    27481 tcggctatgt gcctcaggat cacttcttgt tttcgatgac cattcgagat aatatccgtt
    27541 tttctaatcc aaaactttca gaagaagaag taaagcatgc ggcttcttta gcatttatca
    27601 acaatgaaat caaggaatta ccaaaaggtt atgatacctt agttggagaa cgtggagtct
    27661 ctttatctgg cggacagaaa caaaggattt cgattgctcg agcattgatc aaaaatcctg
    27721 aattattaat gttagatgat gcactttctg cagtagatgc caaaacagaa gaagctatct
    27781 tgcataattt aaaagaaata agaaaagaga aaacaactat cattgcagct cacc
//
LOCUS       DACDYI010000040        26941 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.040, whole genome shotgun sequence.
ACCESSION   DACDYI010000040 DACDYI010000000
VERSION     DACDYI010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.040"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(64..432)
                     /locus_tag="IWM52_001600"
     CDS             complement(64..432)
                     /locus_tag="IWM52_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAP9973854.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            603..1559
                     /locus_tag="IWM52_001601"
     CDS             603..1559
                     /locus_tag="IWM52_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP9973855.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            1632..1748
                     /locus_tag="IWM52_001602"
     CDS             1632..1748
                     /locus_tag="IWM52_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAP9973856.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(1803..3485)
                     /gene="recN"
                     /locus_tag="IWM52_001603"
     CDS             complement(1803..3485)
                     /gene="recN"
                     /locus_tag="IWM52_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAP9973857.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(3503..3952)
                     /locus_tag="IWM52_001604"
     CDS             complement(3503..3952)
                     /locus_tag="IWM52_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP9973858.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(4059..4877)
                     /locus_tag="IWM52_001605"
     CDS             complement(4059..4877)
                     /locus_tag="IWM52_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAP9973859.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(4886..5776)
                     /locus_tag="IWM52_001606"
     CDS             complement(4886..5776)
                     /locus_tag="IWM52_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP9973860.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(5778..6002)
                     /locus_tag="IWM52_001607"
     CDS             complement(5778..6002)
                     /locus_tag="IWM52_001607"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAP9973861.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(6007..7344)
                     /locus_tag="IWM52_001608"
     CDS             complement(6007..7344)
                     /locus_tag="IWM52_001608"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAP9973862.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(7345..8199)
                     /locus_tag="IWM52_001609"
     CDS             complement(7345..8199)
                     /locus_tag="IWM52_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAP9973863.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(8296..8745)
                     /gene="nusB"
                     /locus_tag="IWM52_001610"
     CDS             complement(8296..8745)
                     /gene="nusB"
                     /locus_tag="IWM52_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAP9973864.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(8738..9163)
                     /locus_tag="IWM52_001611"
     CDS             complement(8738..9163)
                     /locus_tag="IWM52_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9973865.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(9409..10473)
                     /locus_tag="IWM52_001612"
     CDS             complement(9409..10473)
                     /locus_tag="IWM52_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP9973866.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            complement(10645..10890)
                     /locus_tag="IWM52_001613"
     CDS             complement(10645..10890)
                     /locus_tag="IWM52_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9973867.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            complement(11029..11403)
                     /locus_tag="IWM52_001614"
     CDS             complement(11029..11403)
                     /locus_tag="IWM52_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAP9973868.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            complement(11486..12400)
                     /locus_tag="IWM52_001615"
     CDS             complement(11486..12400)
                     /locus_tag="IWM52_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAP9973869.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFSPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            complement(12421..13224)
                     /locus_tag="IWM52_001616"
     CDS             complement(12421..13224)
                     /locus_tag="IWM52_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAP9973870.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            complement(13257..14246)
                     /gene="manX"
                     /locus_tag="IWM52_001617"
     CDS             complement(13257..14246)
                     /gene="manX"
                     /locus_tag="IWM52_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAP9973871.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            complement(14349..14840)
                     /locus_tag="IWM52_001618"
     CDS             complement(14349..14840)
                     /locus_tag="IWM52_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAP9973872.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            complement(15031..17865)
                     /locus_tag="IWM52_001619"
     CDS             complement(15031..17865)
                     /locus_tag="IWM52_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9973873.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            complement(18272..18565)
                     /gene="rpmA"
                     /locus_tag="IWM52_001620"
     CDS             complement(18272..18565)
                     /gene="rpmA"
                     /locus_tag="IWM52_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAP9973874.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(18578..18922)
                     /locus_tag="IWM52_001621"
     CDS             complement(18578..18922)
                     /locus_tag="IWM52_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAP9973875.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            complement(18949..19257)
                     /gene="rplU"
                     /locus_tag="IWM52_001622"
     CDS             complement(18949..19257)
                     /gene="rplU"
                     /locus_tag="IWM52_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAP9973876.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            19539..20180
                     /locus_tag="IWM52_001623"
     CDS             19539..20180
                     /locus_tag="IWM52_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP9973877.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            complement(20242..21873)
                     /locus_tag="IWM52_001624"
     CDS             complement(20242..21873)
                     /locus_tag="IWM52_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAP9973878.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(21996..22355)
                     /locus_tag="IWM52_001625"
     CDS             complement(21996..22355)
                     /locus_tag="IWM52_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAP9973879.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            complement(22514..22587)
                     /locus_tag="IWM52_001626"
     tRNA            complement(22514..22587)
                     /locus_tag="IWM52_001626"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22551..22553),aa:Arg,seq:tct)
     gene            complement(22896..23579)
                     /locus_tag="IWM52_001627"
     CDS             complement(22896..23579)
                     /locus_tag="IWM52_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAP9973880.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            complement(23707..23937)
                     /locus_tag="IWM52_001628"
                     /pseudo
     CDS             complement(23707..23937)
                     /locus_tag="IWM52_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320930.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase"
     gene            complement(24115..25308)
                     /locus_tag="IWM52_001629"
     CDS             complement(24115..25308)
                     /locus_tag="IWM52_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9973881.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(25392..25595)
                     /locus_tag="IWM52_001630"
     CDS             complement(25392..25595)
                     /locus_tag="IWM52_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAP9973882.1"
                     /translation="MMNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVI
                     MNGNRIQIKRKQFEKIIDTLDTI"
     gene            25852..26094
                     /locus_tag="IWM52_001631"
     CDS             25852..26094
                     /locus_tag="IWM52_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973883.1"
                     /translation="MAHQPSGSAQTECVRMPYDAPPLSCGHIYQGAIRPPGKHRNGER
                     VSRFMLPGPVRQFVTIAWMSLISRRRFMASSRHTCG"
     gene            complement(26016..26249)
                     /locus_tag="IWM52_001632"
     CDS             complement(26016..26249)
                     /locus_tag="IWM52_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9973884.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(26256..26687)
                     /locus_tag="IWM52_001633"
     CDS             complement(26256..26687)
                     /locus_tag="IWM52_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9973885.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
BASE COUNT         7651 a         6048 c         4668 g         8574 t
ORIGIN      
        1 tttttttaaa aatcaagctg caaaaagcct tttaaaagac tttttacagc ttttctattt
       61 ttttcatagg taagaaagta tgttcacaat gatcaagaca gcgtacaaga cgattgtcaa
      121 cagaaaaacc aaacgccaag tcatcttgaa ataacgcgga taaataattt cttcataata
      181 atatgcttgg aaaacagcaa tacaaattcc cattagcaaa acagaaataa agaaaaacgg
      241 taagatagaa attgtccctg tatttctaga caatgcatgc ataccgcccc acaaaaaagg
      301 aatcgccaaa tcaggggctt ttattttaaa acgacgattt aacgaaaata attttacaag
      361 aaattggcaa ccaaacaaaa cgattaccgg gtaaatatac caaaacaaga taagtggtgt
      421 gaatgtgccc atgaacgtaa actccttttt tatcgttgct tcaagtatac tgtatatact
      481 ttacacaact gtttcatttt atttaaattt atgcagttat attctatatc ttacaaacac
      541 attccatgta ttccaattcc ctatccgtta taataaaaga aacaggcaac aggaggaaaa
      601 tcatgatcaa atattttaca ttagataatg agaaactgcg taataccgga aaggaaactg
      661 accaaacgat ctggtattgt gtagaacgtc ctaatcaaga agagattcat cagctgacac
      721 atcaatttga aattccgtca gattacatca cttccatatt ggacgatgca gaaaattccc
      781 gtacagaaga attcaatcaa tcaaagttga caaagccagc gttattattg ttacaatatc
      841 cttttgctaa aacaagtcct agcggttatt ttcaagtgga tacatacccg ctctctatta
      901 ttttgacgcc caaaaagaag cttatcacaa ttacgaatca tgaaccactg tttttaaaga
      961 aagtctttag ccagaccttt ccaaaaagtg atatttcgcc taatctcaat atcttctttc
     1021 aattcatttg gcaggtcaca ctctcttaca acagttattt agctacgctt caatctcaat
     1081 gcgatacttt ggaagccaaa cttcaagtat ccactgaaaa caaacaactt tatcaaatca
     1141 tggatatcca aaaaagcctc gtttatttca gagaagcgac ttctgctaat ttagatgcat
     1201 tgaaagaact agcgcaaaat aaacttttcc aagaaaatca tgctttgaaa aatcatttac
     1261 gtgatgtatt ggtggaaaca gagcaggcaa tgactacttc tcgcattcaa ttaaaactag
     1321 tagaacaaat gaatcagact ttttcagcaa tcgtatcgaa taatctgaat aatattatga
     1381 aaattttaac ttctttaacg attattttaa ctataccgac aatcgtcggc agtatctatg
     1441 gaatgaacat taagttacct attgctgaaa gggatgatgc ctttatatgg ctagttctat
     1501 taacattatt tatttgtatc gtaactacct attatttaaa aaaagggaag tttttataaa
     1561 aaagccctct tgtggaaatg gaaaaaagat tgtacacttg aatcaactaa ttaatgacga
     1621 ggttgaaaac aatgcgtgaa aaaaaacaaa caagtacttt tgctaaagta acaaaatttg
     1681 tcatttggac aatgctgatt ttaacgattg gatctgttgt attgactgct gtaatgagtg
     1741 tcatgtaaaa ataaaatata gctatcagct gctgaaaagg acctgaacca ctgcgaggtg
     1801 gctcaggtcc tttttgcgag tttcaatagt tcttttgcat gttcgagtgt gagttttgta
     1861 atctcacttc cagccagcat acgtgcaatt tcttcttctc tttcttttgt tttcaactct
     1921 gaaacagaag tttgcgtacg tccatcaatc actgctttca caatataata ttgatagtcg
     1981 gcgactgcag caacttgagg cagatgagtg atgcacaaaa cttgagagta tttcgatatt
     2041 tgagaaattt tatctgcgat ggcctgtgca actcttccgc tcacacctgt atccacttca
     2101 tcaaaaataa tacttgtcac accttgctca gaagaaaaaa tagttttcaa agcaagcaac
     2161 atccgagaca gttcgccacc agaagcgact ttcaccaaag gcttcagtgg ctctcctgga
     2221 ttggtcgtaa tgtaaaattc gatttggtca aatccatcat gattcaattg tctattttct
     2281 tcttttaaga aacgtacctc aaactcagtg ttttccatat acagactttt caattcatgc
     2341 agaatggatt tttccaattc ttttgcgatt tttttacgcg cttcatgaag atctgcggct
     2401 tgttgctgaa gtagaagctc tttttgctct agttcctctt tcattttgtc caattgacct
     2461 tctgaaaaat cagaagaagc tagctcttcg gtgatttcgt catagtaagc caatattgac
     2521 tcaatggatt cgccgtattt acgtttcaac tgtcggatta attctaaacg ttgcgtaact
     2581 tcttctaaac gactctcatc taactccaat aaatcaatct gacgactcat atcgccgatt
     2641 gcatcttgca gcaaatagta agcactttgg atattttcat aaattgcttc atactccaca
     2701 tctagatggg cgatcccttg cagttctgaa acggccaagc caacaccatc tacactgttt
     2761 tgttcattat tcgtcagtgc tccgtaacct gcagctaatc catcagcaat tttttgataa
     2821 ttgctaagtt tttctctttc ttcttttaat ttctcttctt ctccgacttg caaatcagca
     2881 gcagctattt cttcttgctg gaaatggagc atatctattc tctgaacata ggatttttca
     2941 ttttgctgca cttttcgtac ccttttttcc agctcacgat aagagatata ttcttgctga
     3001 taactctttt tcttctgttg gaaagctaca tctccaaaac gatccaataa agcaagatgt
     3061 gattcaggct ggagcagttc ctgatgttcg ttttgtcctt ggatatctac taaataactg
     3121 ccgattcttc tcagattagc taatgtgata atatgaccat tcactcgaca aacatttttt
     3181 ccagttaagg acatatcccg tcgaacaatc agattgtcct catctgtttc gatccctaat
     3241 tcaaccatca gttcagagaa tccttcttgt tttggtagtt caaacaaccc ttcaagcaca
     3301 catttttctg ctccttgacg tatataatcg cttgaacctc ttcctcctgc taacagacct
     3361 aaagcatcga taataatgga tttacccgca ccagtctctc cggtcagcgc ggtcattcct
     3421 tcgtgaaaag aaagccgcaa ttccggaata atcgcaaagt tagtaatact gatttcttgt
     3481 agcatcacat tcgctcctca acttaaaggt aatgcaggaa gtctttcttc aataaaactg
     3541 cctggtcttc agttcgtgta atcatcaaaa cattatcgtc atcattgata atcgaaaata
     3601 attctttctt gtaacgttta tcaatcaaat ttgctgctgc cgaagcattt cccggcaatg
     3661 ttttcaaaat aacaaatttt tccatttggt caactgccac gaaagcgtct tttaatagtt
     3721 tttccaattt agtatttaca tcttcgtttg tttcagctgg tatgctataa cggtagccgc
     3781 catctgaagc cggtaccttg atcagtttca gttcctttat atctctggaa atcgtagctt
     3841 gagtaacaga tattcctttg cttttcagga tttcaacaaa ttcttcctgc tttcggatat
     3901 cttgctcact caacaatcga gtaatcaaac gatgccgatc tttttttctc attacgtctt
     3961 cgctccttag catattcttc ttaatcaatg atagcttatt tccttggtat aataaagaag
     4021 aatccgcata cttctcactt gattttcaca gttacaattt atttatggtc aaactgttcg
     4081 tgtgcatttg aaaccacttc tgccatatta atagctgaat tgattgttcc gctttcgggt
     4141 actttcctca agtgagccaa gaactcgata tttccttctc cccccgtgat cggagaataa
     4201 cttaattcca gcacatcata tccaccttgt gccgcaaatg acaaaatgtc ttctaaaact
     4261 ttttgatggg tctgagggtc acgaacaatt ccgtttttcc ctacagcttc tctgcctgct
     4321 tcgaattggg gtttgattaa agcaatcact tctccctctt cttttaaaat agcatgtaat
     4381 ggaggcagca tcagttttag tgaaataaaa gaaacatcga taacagcaac ttctggaatt
     4441 cctaaggtaa agtcttcggg ttttgcataa cgaaaattca ctctttccat tacgacgact
     4501 ctaggatctt gtcttatttt ccaagctagc tgattgtagc caacgtctaa agcataactc
     4561 atttttgcgc cattttgcaa agctgcatca gtaaatccgc cagtggatgc accaatatct
     4621 aacaatgttt ttccttcaac agatagatca aatgctttga gtgctttttc taattttaat
     4681 ccccctcgag aaacataagg aagtttttta cctttgactc ttaattcact actcactgga
     4741 attttttctc ccggtttatc aaaccgctcg tttttttcgt tgtaaatcaa accagccatc
     4801 actgaacgtt tggcttgttc tcttgtttca aataatcctt gtttgaccgc taatacatct
     4861 acgcgttctt tttccattct ttccctcatt tctccacatc aaattgcttg atcatctggc
     4921 gcatcagccc gccatcaaaa gaaaaaacgt cctcttctaa tttgtctatg atcttgttgg
     4981 cggataacag ctcaatctcc aacgcctcac gtgtcttttc taggcctaat aaacgcgggt
     5041 aagtattttt ttccatacgt tcatctttac cagctgtttt ccctaatgtt ttcgttgtac
     5101 caatcacatc gagtagatca tctcttatct gaaaagctaa acctaaatgt tcggctaatc
     5161 gttgaagtag caataagatt tcttcaggtt gcttcgctaa tatgccacca gccagcaagg
     5221 cataacgaat caaatgccct gtctttcttt catgaataaa tgctaattct tctaaagaaa
     5281 gctttttcga ttcgccctcg atatctgcag cttgcccagc aaccatacct tgtgatcccg
     5341 cacaaacagc taattgctga agcagcagaa gcttaggaga attacctaag tgtgccatac
     5401 tgattagctg gaatgctcct gtaagcaaac catctccggc caatatagct aatgcttcgc
     5461 cgaatacttt atgattggta ggttttcctc tacgaagatc atcattgtcc atcgcaggca
     5521 aatcatcgtg gatcaaggaa tacgtatgca ccatttctaa agcagctgct acttgataga
     5581 cgggaacatc aataggctca tcaaaggaag caacggccgt taaaagtaat aagggacgta
     5641 ttcttttccc gcctgctttc actgaataac tcatcgcatc atagagtcct ctatccgttg
     5701 tatgctcatc taaaaaagac aagatttctt tttctacagc aggtagatga atagctgaaa
     5761 agtcagttaa tctcatctca gctttcctcg tttccgtcaa aatcaacttc ttcatttgat
     5821 tctgtcatca ttttcgtcaa ggtctcttct gctttagcta atgtatcttg gcaatgtttg
     5881 cttaattcca ttcctcgttt aaacgaatct agagctgatt ctaatggaac gtctccttgt
     5941 tctaactgca tgacaatttt ttctaattct tgcagtgact cttcaaaagt tggattttcc
     6001 atatttttat tctcctttgt ctatctcttg tacttgtcct ttaacgattc catcttgata
     6061 atggatcgtc agcaaatccc cttgatgaat ctcggtgaca ctttttacta cttgatcatt
     6121 taacgtcgta tagctgtagc ctcttcccat gattttcaat ggactcaaca aatcaagaga
     6181 ttgaacagct tgagcaaaac gctgctgctg ttgtttcata tattgttgga ttttctcttc
     6241 taagcgtttt tctagataag cagtttcttg gcgagctgct ttaactcgat aaagcggagc
     6301 cacttgttcc aattggtggc ttaatgcgat cgtttgtttt tctttcgcat gataaatttg
     6361 ctgtgtagct tgatataatc gctgtttcaa ctggtctaat ttgatggtct gaccttcata
     6421 caatctttca ggctgccgaa atacatagga acgtcgagca cgctcgaacc gttcttgttt
     6481 gcgctggatc tggcgtacaa acgcctgctg caatctagct tgccgttctt gaatgcgcat
     6541 caactcttct gacaaaacag gaacagctaa ttctgcagct gctgttggag tggcagcccg
     6601 aacatctgct actaaatcag cgatcgtggt atctgtttca tgtcctacag atgaaatcac
     6661 cggcgtatta gcttgataaa tcgcccgagc aacccgctct tcattaaacg gccataaatc
     6721 ctcaatcgaa ccacccccac gaccgatgat cattgtatcg aaattgccca tctcttctac
     6781 tctctggata ttcctgacaa cattgtccgc tgcttggttt ccttgtacaa ctgttgggaa
     6841 caaaaccaat tggacaattg gatatcttct tttcaccgtc gtgatgatat cgcggataac
     6901 cgctccgctt ggactggtaa ggaccgcgat tcgctttggg aaacgtggca gcattttttt
     6961 aggcccttga aaaaggcctt ccttcatcag cttttctttt cgttcttcca attcttgata
     7021 taaagcacct attccatcag gctccatgtg ctctacatag atttgataag aacctcccgc
     7081 ttcatataat gaaatacgcc caacgacaag gactttcatt ccttcttttg gctggaatcg
     7141 taatttttgg aatgctcctt tgaacataat agcagatatt tttgcatggt catcttttaa
     7201 gctgaaatat tgatgggcat tggcacgtaa acggaaattc gatatttcgc ctgttaaata
     7261 tacgcgttct aaataaggat cggcatcaaa ttttcttttc aaatacttcg ttaatgtagt
     7321 gacagttaaa tattcttgtg ccatctattc acctgctcca aaagcttcgg cagattttac
     7381 ggtttgatac atcagcatcg tgatagtcat tggccctaca ccctttggca caggagtgat
     7441 ccagcctgca ataggctcca cttcatcaaa tttcacatca ccgatcagct taccgttagc
     7501 atcacgattc atccctacat ctatcactac agcacctggt tttacgaatt cttttgtgac
     7561 aaaatgtcca cgtcctattg ctacgactaa aatatctgct tcttgtgcca gttcagccag
     7621 attttcggtt ttagaatgag caacagtaac agttgcatct gccatcaaga gtagctgagc
     7681 catcggttta ccgacgatat tgcttcgtcc gataactact gctcgtttac ccgccggatc
     7741 gatagaataa gcttcaaaca ttttcatgat gccgtatgga gtacaaggaa tcttgtccgg
     7801 tttacccgca aggagtctgc ctaaattcat cggatgaaaa ccgtctacgt ctttttcagg
     7861 gcggatagct aacaggatct tttcttcatc tatatgcttt ggcaatggta attggaccaa
     7921 tataccatga aaactaggat cttgattgta tttttcaatt tctgcaagta gttcgtcttc
     7981 agaaatcgtc tcaggataac gctctaccaa tgaatggatt ccgatttctt ttgctacacg
     8041 ttctttattt cttacataga tttggctggc tggattttcc cctaccaata aaacgactaa
     8101 tccgggatgg ataccattat ctttcaattt ttggattttt tcagcaatct ctgcctgcat
     8161 tttttcagct aattcttttc cgttaatcaa ttctgccatc taaccactcc cagatatttt
     8221 ttgtgttttc attctaccat agaattttcc cgatttataa caggtttatg atgattcata
     8281 gaaaaaagct aggacttacg ccccagcttc taattctttc aatacattgg ataaaacacc
     8341 gttgacgaat ttacgtgaac ggtcatcact aaatgttttt gccaactcga ttgcttcatt
     8401 taaagcaaca gccgctggta catctgtcac ataagtcatt tcaaaaatcg cgatacgcaa
     8461 gatcaccaaa tccatttttg agatccgatt gatgtgccaa ttgtttttta aatgtttttc
     8521 aatcacttta tccaattcat ctttttttgc acagacaccg ccaactagcg tatcaagata
     8581 aacaggaaca aactctgatt catcttcgtt gatcatgtcg cggtggtcta attcgattgc
     8641 gttgaaaatc gcatcttctt ttgttaggtc tgcattgaag tctaatggaa ataaagcctg
     8701 caatgccatt tcccgaatct cgtgtcttga taattcttta ttcattctct tcctcttcgt
     8761 cttcaaaaag ttcttcgatt gttggttgtt ctaatttttc aggaacaaca gctactacat
     8821 ggatattgac ttcagataaa tcgatatccg tcatgaaaag gacttgctgg ctgacccgtt
     8881 cctgcatttc cattgctact ttaggaactg aaacaccata ttctagataa cagtaaagat
     8941 ctactttcaa gccatcttcc tcgtttgcta agtaaacacc ttttccgtga gctgcacgtc
     9001 ctaataattc agtgacattg ctggcaaaag ttccgcgcat tccgtatacg ccttctactt
     9061 tagaagctgc aatcccgata attacttcga tgacttcagg agcgatcacg atctctccta
     9121 aatcttggtt tgcgtttaaa atcaaatttt tttcatctgc catttattta ttcctccctt
     9181 tgcctaagca cgagatacat ccgaattctc tatgatgcgg catagtgatc acgtggtaag
     9241 aaaactgccg ccgatcagct gcaaactgct atgaattgga gaaaacaccc aactttatta
     9301 tctcttacag cttaaaagtt cttctatagt gtatcattaa atcgagtgat tttctataac
     9361 aaagtctgtg aacactgatt tttacgctta cttcttctat tgatgaaact ataagatgat
     9421 caattctttt ggtgaacgag tcaacacacg atttccatct tctgtgatca acaagtcatc
     9481 ctcaatacgt actccaccga ttcctggtaa gtagatccct ggttcatcag tgatcacatt
     9541 tcctggtaca aattgctttt ctgcacgaga agaaacgttt ggtccttcgt ggatctctaa
     9601 accgatccca tgtcctgtag aatgtccaaa tgcttccccg taaccatgtt ttgaaatata
     9661 atctctagca actgcatcta attggacacc agtcattcct ggttttgcag catcaataac
     9721 tgctaattga gcatctaaga ctatttgata aatttctttt aatttttccc caggatcacc
     9781 aatagcgaat gtacgtgtca tatcggaaac gtacccttcg taatagcaac caaagtccaa
     9841 tgtgatcaag tcaccttgtt cgatgatttt tttgcttgct acaccatgcg gcatagccga
     9901 acgcaatccg ctagcaacga tcgtatcaaa agaaacacct gaagcgccaa gagaacgcat
     9961 ataaaagtct agttgatttg cgacttcaat ctcagtcatg cccggttgga tcattttcag
    10021 gatgtgatca tatgccatat ccgcaatatg acatgctttt tcgataatag cgatttcttc
    10081 ttcatctttc acttctctga gttcttcgat catctgtgaa acaggtacta attccgcatc
    10141 gatgatttct tctaaaaccg aatattctaa aaatgagact gttgtttcct caaaagctaa
    10201 tgagtgcagt tcttctttgc gtactaagtt tgctacctca tcaaaaatag gtccgacatt
    10261 tttaataatt tcaaaccctt gagcctgcgc tgccgcctgc tcggtataac gaaaatcagt
    10321 aataaaaaat gctttttcca aagtaataac agctaatcct gtcgtacctg taaagttcgt
    10381 taagtaacga agattgtacg gactggtaac caaaaatgaa tccaaatttt cttcttgcat
    10441 ttttttgcgc aatttttcta cacgtagcat cataatgaaa ctccctcttt ctttgcacgc
    10501 ttttttccat tatagcaaac ttttcttcgt acacgacagt taaataaaaa tttccggaaa
    10561 acttcttttt attttaaata aataaagcca aaaaggagtc acagtctcgc tgcaactcct
    10621 tttagtttac tattttgtaa aaacttatga tcgttttcca aagaaaaatg agacaactgc
    10681 taccaaaatc actgcgccta aaatagatgg aataagtgcc atacctgcag cttttggtcc
    10741 ccattcgccg aaaatcgctt gtccaatact tgaaccgact agtccagcaa tgatattcgt
    10801 tacacagccc atcgaatttc ctttactggt gatcgcacct gcaattgctc cgatcacggc
    10861 tccaacaatc aatgtccata accaactcat aatataacct ccttctttgc taatcaattt
    10921 tagcatatgg gatttaaagg ggacaaataa ttggttttgc tatttcatat tttcttctgc
    10981 aaagtaaaaa aataaagaaa acctcctctt agaagttttc tttatttttt acttgcgttt
    11041 aaacatacta cgaataccac gtttcactac atcaaagaaa ctcaatgatt gtaccattct
    11101 atcttcatct acatattctc tgatcgcacg aagcactttt ttaggttctt tggaagaaaa
    11161 tgtgtatgta ccattttttt tcgtttgaat ggcatatcga ggaatccatt ttccctttag
    11221 catgactgat gctagtacat aatccacttc ttcccatgga atttggatat atttgcgtct
    11281 atctcgcgca tggtaaaact caaaaccatt atcacctatc atgatttgcc cgtaatctgt
    11341 cagacccgta aacgaagtag catcaattac caagtctact tttgtattga ttgattgaac
    11401 cattttctcc ctccagatat ttgtttatga acagtatact atataaaaaa agctcgggac
    11461 aacgcccgag ctatattttt atggattata aaagtccgat gacgtgtcca acaacaccga
    11521 ctacgaataa accaaggatg ataacgattg gagaaacctt tttcttaagc aaccacatac
    11581 ataacaatgt taatcctaat gcagctaaac caggaatcaa ttggtctaag ttgtcttgta
    11641 atgttgttac cttttcagga gaaagggcta agccattatt aacttgttca aatgcactct
    11701 tgattccttc accacctgaa ggcaatttat cccattcgat gtaagcacct ttatctaatt
    11761 ttacagttga tacaactggt gagaatttga ttgataccca tctttgtaca agggcagcta
    11821 atacgaacat ccctaaaatt gaagcacctt ttgttacgtc ttgtaacaat ccgccagaaa
    11881 ggtcatcagt aattttagaa ccagctttgt agccaaattc ttgtgtatac cacatgaatg
    11941 cccaacgaat aatgttccaa cctaagaaga agatgattgg tccaaggata tttcctccca
    12001 tagctaatga agcacctagt gcacctagca ttggacgtac tgtaaaccag aacactgggt
    12061 caccaacacc agctaaagga cccatcatac ctactttaac cccttgaatc gctacatcat
    12121 caacaggcgc gccattcgca cgttcttctt ccagagccaa tgtaacccct aaaattggag
    12181 aagcaatgta tgggtgtgta ttaaagaatt ccaagtgacg ttttaatgct gcagaacgtt
    12241 cttcttttgt tttgtataat tttttgattg ctggaatcat agaaaatgcc cagccaccgt
    12301 tttgcatacg ttcgtagttc catgaacctt gaatgaaggt agagcgccac gcaacagcta
    12361 aacgatcttt ttttgataat ttgatttttt cttctgccat tttattttcc tccttcaaga
    12421 ttaatagtca ttcaaaatgt cgcctaaagg gtcaccagag ttgcttccgc ctccattatt
    12481 tgatgaacca cccattttag aaaggttcaa gtaaataaga gctaatgcaa cacctaaagc
    12541 acctagagca atcaatgtta attgagaaat tgcagctaca acgaaaccaa taacgaagaa
    12601 tggccatact tcttttgtag ccatcatgtt gattaccaaa gcgtaaccta cagctacgac
    12661 cattccccca ccgatagcca taccatctgt taaccatgct ggcattgatt caaggaatga
    12721 ttgcacagtt tgtgctggga taaatagcaa cgctgctgct gggaccgcga tacgaacccc
    12781 ttgtaaacat acagcaatga tgtgccacat ttcaacctta cggatgtttc cttcttcagc
    12841 agccgcatcc atcaaatgaa cgattggaac agcgattgta cgtacgatca ttgttaagaa
    12901 caaccctgca acagccaaag gaacggcaat cgcgatcgca gaaggaacac ctttaacacc
    12961 ttgtccacct aaaactaaaa taattgctga tgctactgat gctaatgcag catctggtgc
    13021 tacagcggca ccgatatttg cccaacctaa agcgatcatc tgaagcgtac cgccaagtac
    13081 gataccagct tcgaggttac cggtaactaa accgatcaat gtacatgcca ctagtggttg
    13141 atgaaattga aattcatcaa gaataccttc cataccagct agaaaggcaa ctaatacgac
    13201 taaaattatt gaaataatag acatgataag cctcctataa attttaatat tatttattac
    13261 gcttttgcta attcagcttt tgcttttttc aaaatatcat ccatgttatc gcgtgaatca
    13321 ttaggtactt tacgtacatc gaatttcaca cctttttgtt caagcttctc aaacgcttca
    13381 acgtcttctt gtcccataga caatacttta cttacaacga ctttaccgac cgaatgcgcc
    13441 attgacccaa catttaattc tttgatgtct acgccacctt caacagctgt caatacgtct
    13501 tcaggatttt caaataaaag caatgctttg gtatttccaa aacgagggtc ttttgctact
    13561 tcgatcattt tgctaatagg aataacattt gcttttacac ctggaggagc agcttgttcg
    13621 attagtctct tacgcagatc atcctttgat actgcatctg aaacaacgat aatacggttt
    13681 ggttgcactg cttttgtcca agctgtcgca acttgtccat gtaacagacg tgagtcaaca
    13741 cgtgctaata catatttgat ttttccgtct ccgaccacag ttccttctgg aagtgctcct
    13801 ttaggttgtg cgtcttcaac tgctgctttc ggtgcttctg ccggttctaa ttcttccggt
    13861 tttactttta caccgtcttt tgctgtttca atgatgtgtg ctgcaatttc atgagcagat
    13921 tccattgaaa agcgtgatgc gtaggcttca atcaacattg gtaaattcaa accgctgacg
    13981 attgcccatt tatctttgtg ctcttcgaat aatgtgtttg attgattgaa cggtgttccg
    14041 ccccataaat caactaagaa taacacttcg tcttgattat caaaagaagc aaccgcttct
    14101 tccaatttgg ctctcaagtc atctggacct tcacttggtt tcaaaatcac ggctttgacg
    14161 ttttcttgtt cgccaaagat cattgaaccg gattgtaaga ttccttcagc aaattgtcca
    14221 tgacttgcta ggataatccc taccatattt atacctccta tttacttttg ttgtaacaaa
    14281 ttcaaaaata ttaataattg ctaaccatta caacagaatt atttgacctt ttccaacaac
    14341 cattcttgct acaccttttt gattttcgct agtagttcca tcaaaaggat tttcctgtca
    14401 gcaggtactt ttcgaatctc taactccact tcttgactgt tcaagtaacg aaaggaatca
    14461 atatcttgtt gatttactga aacaaaattg gtaatcattg atttgccttc agtaaaactc
    14521 ataccgccga tattcaacga cttcaactgt acaccgcagc ggacaaccgc ttcaacatct
    14581 tggggattgg tgaataaaag catcactttt gctcgtgata ataatccact atgatagatt
    14641 tgaatcattt tctgcacggt tattacattt gctgtgatcc ccggcggtgc agcttctttt
    14701 agtagaaact ttctcaattt atcttgagcg actacatcgc tgatcacaat gatacgttca
    14761 acacctgttt gcttcgacca aactgttgcg acttgcccgt ggattaaacg atcatctata
    14821 cgtgccaatc gaatatccat ctgcaaaact ccttcttcct cactacttca cagaaacccc
    14881 tatcatttct gtgtgaacaa ccacctccta gcacacacta ctataagcaa aaagcgtgcc
    14941 aactttttta cgatacacta attgaattta aaggattctt aaccaataca caaaaacgat
    15001 acactataaa agtgtatcgt ttttgtgtat tcatggcaca gtgtattctt ttttatctaa
    15061 ctgattatgg actagacgta gcagataata catttcttct atcggcactt ttacttttat
    15121 ctgatcttcc aacagaacaa ctgaggctgc caattggcta taaagaggat cattcgttgt
    15181 atttgtcaat tcgttttctt ccacagtcaa tggttcattc cgaataatac gttcgatcat
    15241 tcctgctgtg tgcatcacta ggttgatttt gaatccatat tcttcatcgc cgattcccgt
    15301 cgtctgagag atttgtgtag aaaattgcca cattggatcg attaacttac taccattaat
    15361 aaagatgaag ttatcagaga taaactcaac acacgtattt ttagctgact cttcatctag
    15421 ggagatgaat tcagtttcct ccaattctga ttccatcagc aactgatcca aaatgacttc
    15481 gatattttga tcaataaaac gttctaaagg aataaatggg gcttggatat tcaaatcagt
    15541 aatcccagta gtagctatga tctgatattc tttttggata actggcagtt cttctttcac
    15601 ttccacaatc gacaaagcta gaacttctaa ttctgtttct tgacgcttgt ttaccgctcg
    15661 ttcgatcaat tctttgattc tttgtgctgt accttcgcca gatgcgcaga tagccaaaac
    15721 agcttttttc ttatgaatag gttttgcttc ctgaacagat tctacagaag atatttctgc
    15781 gtaacctcga aaatttttca atgattcatg caacatatct agatctgtcc ctaatactga
    15841 gacctttcgt acagtttcca aaactagaga agttgttacc atatcaacag tacgtactgg
    15901 aattccagtg tctctttgaa tttgcgaatt aaacgacgca agggacccca tatctactaa
    15961 gagtatgacc ccgcttcctt catctacttc ttgtacattg cgttcaattc ggtccaatgc
    16021 tgttttcgga tccatatcca aaggcatgtc tactgctcgt acgttctctg catctaacaa
    16081 ctgttggaca acttggacca tactgcttgc tgtgctgttt ccgtgagctg caacgactac
    16141 accgattctg ccgcttgctt gatttgcccg taacgagacg agtagaacgg tcaagtaata
    16201 gtcttcactt tctggaatct ccacttgaaa atacgtttcg atataactac gcacttcttt
    16261 cgcaacagca tattcatctg gaaaatcaaa agccatctca cggatgttat cattcgtatg
    16321 ccgttcttct cctaagttga tttttttcaa aaaagaactg atatgcaagc tcatagcata
    16381 aataaagttt tgctgaaact catatggtaa agatcttttg accattacat aaatctgatt
    16441 ggtcacttct atgattttcg gatctacaaa ttctgctaat ttgctgtctg cattaaaaga
    16501 aaaaccatgg tctttataga aagacttcag atggatgttg atatctgtag aaatgaaatg
    16561 attgattgct tcttgatcca aaccatccga cttcagtaaa gctgctttat ctccgatgat
    16621 atcatataga ttataaggta actcatagga gtccgattgg attttcatga tcgtatcatt
    16681 tggtgctaca gttatctttg gctccaatat tttagaaagc tctgccattg cactgcggtt
    16741 gcttgctaat tgaatcaatc cgctacgaat cccttctgtc aaatcatcga ttgtgatcga
    16801 gatttcgggt gattgcatat gatttagaaa tcctcgtgca catactaact ggatatttga
    16861 cttcaattgt cctatatttc cataggtcac actgccgatc aatgcttttg ctacatcttc
    16921 tgtcaaagaa atctttcgct gcgtgcggtt tgcttcgtgg gcaatcatga tttttactaa
    16981 atcgactttc tcatttgcag gacgtttttc aaaagccgga agtttgatat tgattgggat
    17041 acgccgaaca aaagtttcta atagagaaga acctgggtct tctgtggtcg cacctacgat
    17101 ccgtacattt gcttcatggg atttcgttgt ttccccaaga cgactgtaag ttccatgatc
    17161 cataaagtaa aagatcattt cttgaccttc aggcggcaac cggtgaatct catccaaaaa
    17221 cagcataccg ccatccgctt gatcgataat ccccattttt tcttcatctg cgcctgtaaa
    17281 agcacctttt gcgtacccaa ataaatgact catcaaaagc tcagggttat tggcataatc
    17341 agcacaattg aaaacaatca gttccgtttc ctcatctatt acatgatgcg cttttgcaaa
    17401 atgatacatc gcgtgtgcaa aataagtctt tcctgatccg gtaggtcctg taatcaaaca
    17461 atttaatcct cttggcggat acaaaatcgc agcttttgcc tgttccactg aatttttcat
    17521 gctgccgtta gctccaatga tttttgcaaa gatatcctta gtatctattt ggagtgtatt
    17581 tttttcttca gagaccggcg tttctttgta actgactaca tgttttgtaa ctggctttac
    17641 gtgcgtaacg agtgtttgat atatgtaacg tactggtcgt ccgtctgtct ttatcagttt
    17701 tccttcgcgc actagctgat tcagatcttt actgctattt gttcgctgaa tccctaacac
    17761 ttcagcaatt tcctgtgttg ttactccttg atcatattct gcctttgtta aatgagcggt
    17821 tttttctttg acatacatat aaattcggtc aatgcgtttt gccaatggta tcgctccttt
    17881 tctatagact attgtactta ggcttcaaaa cataatacta tgtatttatt tctgtttcgt
    17941 caactattta cctgattttg ttttgttgat aacacgcgga tagatagaaa tgttcaattt
    18001 aaaagaacga aaaatcccga tacgattttt ataatggaaa caagaaaaag aacactgttt
    18061 accatataat tatttcactg atagcaaaca atatagcttc catcaattga aaacgatagt
    18121 tatcaaaaca tcctgattcc aaattggcaa aaaaatatta agtgtaaaaa gacaaagtaa
    18181 acttcgtagc ctccactaca acttttattt ttataaacaa aaaaggctgt gacaaaagtc
    18241 ttgtcacaac ctctttaatc ataagattca attaagcttc ttgagctact ggataaacag
    18301 acacttgttt tttgttacgg cctttacgtt cgaaacgtac tacgccatca acttttgcaa
    18361 ataaagtgtc gtctccaccg atacccacgt ttacacctgg atagattttt gtaccgcgtt
    18421 gacggtaaag aattgatcca cctgtaactg tttgtccatc agcacgttta gcacctagac
    18481 gttttgattc tgagtcacgg ccgttagatg tagaaccgcc accttttttg tgggcaaata
    18541 attgtagatt catagataac aacatggtct gcacctccta tttatcatta attgttttaa
    18601 tttggatata ttctgagttt tcagcttcta tcgattgtaa tcctaataaa agattttcta
    18661 atagaatctc agcaatattg ttttgttctt gtgtcattcc tgaagtgact tcggtataaa
    18721 gataaccgcc ttcttcttca ttcatttcaa caatcggctc aaaaccggct aatgaggcaa
    18781 tcccgttgac agtgctgatc gctaacgcag aaacacccgc acaaacgata tcacttccat
    18841 aaggaccagc atcagcatgc ccggtcaaag tgaatgaaac gatctgacct gcgtcatttc
    18901 gcttaaatgt tcctttgatc atatataggc cttctttctc aaacgctatt aagcgttgat
    18961 cgcgtcgata accacttttg tatatggttg acgatgacct tgtttacggt gtgaatgttt
    19021 tttaggtttg tatttgaaag taacgacttt cttttgtttg ccgtgttttt caacagtacc
    19081 ttcaacagtt gcacctgcaa cagttggagc gcctactttt gtagattctc cgcctactaa
    19141 gataacttcg tcaaaaacaa ctttttctcc tgcttcaacg tcaagttttt caacatagat
    19201 tgcttgtccc acttcaactt ttacttgttt accaccagtt ttaataattg cgtacatgct
    19261 ataaagcacc tccttctgat atacttagac tcgccatccg aagtgacagc atatgctgtg
    19321 cttattactt gttctgagcg gttgtagctg tggagtccac aattacaaca gtactagctt
    19381 accagaacag caaccgttag tcaactaaat ttaaaatcaa aaagtaaaac aaaaattcaa
    19441 gcaatacttc tgcttttcta tcctcacagt tctaccaaat aaaatagtcg cattgtgttt
    19501 cttttattct tataataaaa gaaaaaggag gagcataaat ggaataccca aaagaactga
    19561 tacaagaagc ttctttaaga atgaacggcc gtcctgtaga aggatttatc gctgggcaag
    19621 gacctcttca tcctaaactg atgctcgtgg gagaagcccc agggaaaaca gagattgata
    19681 cgcatgttcc ttttagcggt caagctggaa aagaattgat gcaggcactt tcttcaactg
    19741 gcttaacacg agaagaggtg tatatcacca gtgctgtaag gagccgcccg tatcgcgtga
    19801 ctcatcggat caataaacgg acacaacagt cagaaactgt ttatccaaat cggacaccta
    19861 ctcgttccga agtatttgcg catgcaccaa tattggatta cgaactgatg aaggtccagc
    19921 caaaattaat tgcgacactt ggaaacattg gtcttcaacg tttacttggc aaagaatata
    19981 cggttaccaa aaatcatggt caactctata cagggcctgt cttacaatta acagaacaaa
    20041 aggatgccta cgaatggtca gaaaaaaact atcgaattct tccacttttt catccagcag
    20101 ctattttcta caatcgctcc cttgtacctg ttatccatca agactggcaa gtactagctt
    20161 cacttttaca gcaatattaa aaagaacaga caagaccata aaatcttcag tttacagtct
    20221 tgtctgttct ttttcattgt tttaatggct aattgcagta tatttcgcat gtaaagctgt
    20281 caacgcatga gcaactgttt gatgttgatg cgtgaatgta tgttcccaca agtgagaaag
    20341 ttgtttttct acttctggct gcaaggaagt atagataatc gtttgtcctc tttgctctgc
    20401 tttttcttga aattcatcaa aaactgcacg tgccgtcaaa tcaatacttg gtacgcctcg
    20461 catggagaaa atgacaccat cgtgatgatt tagttcttcc aatttatttt tgagtttttc
    20521 tgaagacata aaaaacagtg gaccactaat atacactact gtccagttat tcagcttttc
    20581 cgtttctggc agccccattc tttgccaatc gatcgtttct aatgaaatcg tgatagcagc
    20641 gcttttgaca acaaaaaata cacagccgcc gatcaccccg atcacgatag caatacttaa
    20701 atcaaagata actgtacaaa tcatcgtaag gataaacaat agaactgctg accaatattt
    20761 attagtaaat agctccttga tcgtttccca ttcattcata cgccaagccg tcacaatcaa
    20821 aacacctgca agtgctggca ttggaatctg tgacatcact ggcgcaaaaa caagcatgga
    20881 gagcagtaag acaactgcat gtatcatacc agctaatcta gtttgtgcac cagatttgat
    20941 ggccacgctt gtacgggcga tggccgcagt agcaggaatc ccgccaaaga aaggaagcaa
    21001 cagatttcca attccttgcg ccattaattc ttgattgcta tctaagggct tatttgccat
    21061 tcggccagcc gaagcaccac atagcaagct ttcaatcatt cctaataaag caatgctaat
    21121 agctggaact gccacttgtt gcaaggcaga aaagctaaaa tctcccatat tcaaacgatt
    21181 actactgatc aatgtttgtg gaatcttacc cacagttgct actggtaaat ggaacaatag
    21241 cataactgct gttgccaaaa tgatagccag cagcgaacta ggcattcgtt tcgcccattt
    21301 ttttggatag ataaacatcc ctaaaatgac tagccctccc atgatcaaag aggctgtgtt
    21361 tatcggaaat cctatcgttt gataactact tagtttttca ataacgtttg taccttccga
    21421 gtgaacacca aataaattat cgatctgacc taatgcaata atgatagcaa tacctgaagt
    21481 aaaccccgta attacgggtg ctgggataaa agaagtcagt gttcctaatc ggaaaagtcc
    21541 tgctgcaagt aaaaatagac ccgctaaaaa agtagcaagc aatacacctt gcattccctt
    21601 tgttgccgca atagacatca agatagctgc catcgcacct gtcggacctg atatttgata
    21661 aaaaccgcct gataaaccgc caataacgat accggcaatg atagccgtaa tcaaacctgc
    21721 ggccgcatca gctccgcttg ataccccaaa agccaaggct aaaggcaatg ctacagcagc
    21781 tactgtgatc ccagccaaaa gatctttctg aaatcttgaa agatcataac ctgaaaattc
    21841 tttttttagt gaggaaacat aatttttgaa cacctatcta actccttttt catgaaattt
    21901 tctaaaatac cattcattag aatatcatat ttcatgagat aaagtaatat ttttcacaaa
    21961 gtttcctgtt agataaattc ttattttttc tatatttata agaaaataga aagcaatgcc
    22021 aacagagcta gcccaccttg tttgagtaaa atccaacgat cgctagtcaa actgccatat
    22081 aatgcaacaa aaaaaataga tattaatagc atacttgtga tttctttaga tgcggaagaa
    22141 agataagctc cataaagtag tcccactcct aatagtccat tgtacactcc ttggttctta
    22201 aacaatgttt gaacagattt tctgcttaac tcttctttac tcattttaaa aaccctactt
    22261 gtcgtgtcag aatcagttct caccgtttcg atatacatga tataaaaaaa ttcaattgca
    22321 actagagtca ctaaaattct agatagtatc gtcatatctt cttcctcttt tctgatgagt
    22381 tttatttttt tgctccttat aattgtactc gataaggatg tttttttatt ttatttgcct
    22441 ttgaaaacaa acaatattgg cacaaaaaac aaagaaaaac cgttagaaac ctcagtttct
    22501 aacggttttt tagcgtcaca ggagggattc gaacccccga ccgtccgctt agaaggcgga
    22561 tgctctatcc agctgagcta ctatgacaag aatcagcgga acatcaataa ttatagataa
    22621 ttaaattgct tctgtcaata cgaaatagtt atttaattag caaataatga attattttgg
    22681 cccaactact aaatcaatct tttaaaacga gacattctcc ttcagctgtt ctaaggttat
    22741 caattgaaaa caggagctac attgacttca tgctactcct gtttatttag atttcctgta
    22801 tcctttaatt gacttacaaa tatagaaact tttacatcaa gttcgaattg agccctcgat
    22861 tcaatcgatt atctacaggc tgccataatc atgtatcaca actcgattgt ttctgattcc
    22921 tttgtcatta agatcacacc atcacctaat ggcaagattg tcgatgtcaa atcgggatga
    22981 tccatcacaa cacgaagaaa ttcatttaat ttacgatgga tagctcgttt gccacgggga
    23041 atctcttcat ctgggagcaa gatcgttcct ccttggaaga tgtcatctac catcaaaacg
    23101 ccaccttttc ttaacaaacg taagcattca ggtaaaaatt caatatattt tgattttgca
    23161 ctatccataa aaataaagtc atacggacca gacaactccg gcaagatatc tgctgcttga
    23221 ccttcaagca aagtcacttt atctgtcagt cctagacgtt catagttttt tttagctttc
    23281 tcgatcatga catcgaaacg atcaattgtc gtaacatgag cgttttctcc gattgtcgtg
    23341 gccatgacac ttgatgaaaa accgatagct gctccgatct ccaagattcg ttcaggctga
    23401 agctgtccaa gtaaaaattt caaaaataca actgtttcat ggggaataac tggtacttct
    23461 gctacatgag cttcttcttc cagtttgcct agctcacccg gtaatttttt ttgctgggta
    23521 cgcataaaat ccaatagttc ttctttgact acaggacgat gcatcatttc attgcgcata
    23581 ctactcctcc tcttcacaca ttatacgaac aactgactaa atctacaagc tgcatgagtg
    23641 ttttctcgtg tttcatgttc agaagagtcg ttggatctct ggaatacatt tatgttttta
    23701 acattcttac aaaaatttct gtgttcgtct ccgcttcttt ttcaactcta caaaggtatt
    23761 ccatgctgtg attcctgtta ggcaaaaagc aatcgtaaag aataacccct gaatccatgc
    23821 aaatgccgtt tttgaaagga ctagtccaat atatggaatc tgaaagatgg gactaccgat
    23881 aatatttctc ttttttacta ctccaggctc aatataagtc tttgtatcag cttttgagga
    23941 aaaattatat gggattttag attatctttt tagaagttct tgaatgtcgg tatagcccgt
    24001 gtttccgggc ttttccggca tttttgatat tgttagatac ttacaaatgt tcttattacc
    24061 tctcaccatt tccccctcaa aagtagtaaa tggagtagta aaaccggcct tgtgtcaggc
    24121 tgccagccgt tccatttctg cccttgcgga atcgaatgtg gcgtgtgcat aatagttcag
    24181 cgtcatggtg atattggaat gtcccataat gtactgcaag gctttggggt tcattcccgc
    24241 attggctaac cgggtgcaga atgtatgacg catagcatgg ggcgtcgtcg ttttcggcaa
    24301 cgcttcttca tggctcttgt tgtacttctt cacaagcctg ccaaacatcc cgccataatc
    24361 taccgccgtc atgggatagc cgttctgttt gagaaacagg aaattggcat acccgtcaat
    24421 aatgaacggc tttgcacctt tgcggttttt tatcactcgc tggaacgcct gatacacttc
    24481 ctcattcatg gggatttttc tgacgccgct ttgggtcttg ggttcgtcaa tatagtaacc
    24541 gacgccggta tttctcaaaa gctggtggct cacgatgatt acccggtttt caaaatccaa
    24601 atctttgtcc gtcagtccgc acagttccga aatgcgaagc cctgtcccca gcagaataac
    24661 gattgcgtca taatgctttt ggtaaacctt atcaccttga ataaaggaca gaaaactttc
    24721 ttcctgtgct ggtgtaagag gcacctttgg ttccgtatcg tcgtccagaa cgtcactaag
    24781 ctggaagtca aacgggtttt ttcggatgca atcgtcctgt atggcggtgt aaaaagccgc
    24841 tttcagggaa cgcttgtcgt tattgatggt cttataagaa aggccctttt ctttcatccg
    24901 aagcgcccat tcctttgcgt cggacagctt cacactgtca atcgggcagc tccccagccg
    24961 atcttgttcc agaatacgca tcagccgttc ccggcccagt ttggtgttgt gcttcacatt
    25021 gccccggtgc ctgatctgct tggcgtagag ctggcagacg gtcattttct tgccgatggt
    25081 gtctatcccg tcgtcaaggt ctttctggat ttctttctcc ttttcccgga gtgaaatatc
    25141 ctcccgttta cctgccgggg tcttatccgt aggcactaac ttccatgagt aaacaaactg
    25201 cggttttcca aaggtatcaa catatttgta agcatatcgt ccgtcttttc tctggctctc
    25261 tccagaccgc aaaacgcgat ttttgctgtc tcgtcttttt tctgacatct cgtcatgctc
    25321 ctttccatga cggaaagagc cgcatatatg cttataaatt attataacat atctgcggcc
    25381 ctttttcact attagattgt gtccaaagtg tcaattattt tctcaaactg cttgcgtttg
    25441 atctggatgc ggttgccatt cataatgacc cagccagagg ccggattttc ctcggccagc
    25501 ttacgcaatt tgttttctcc gatacggaaa tatttagacg cttcctcaat cgtcagcgtg
    25561 tatttttccc aaataggcac atcatggttc ataatgcagc cccctttcac aaattcaaag
    25621 gtttgtgtga tttaggggca tagacggacg gctgctggca cagctccacg ggactttcac
    25681 ccccgcgtgg ttctcacgcg gccctaccca ttgcctgcga cgcttttaac gctcggactg
    25741 tggctataag ggagtatcat agtcctgcct gtacgtcgtc gcccgcaagc cgccacgctc
    25801 acattgcggc gtggtgttcc tcgctccgct tttcccgtag ggaaaagtat catggcgcac
    25861 cagccgtccg gctcggcaca gacagaatgt gtccgtatgc cctatgatgc gccgccgtta
    25921 tcttgcgggc atatttatca aggtgcgatc aggccaccgg gaaaacaccg aaacggggaa
    25981 agggtaagcc gtttcatgct gccggggccg gttcgtcagt tcgtgacgat agcgtggatg
    26041 agcttgattt ccagacggcg cttcatggcc tcgtccagac acacatgcgg atagccgttt
    26101 tcgtcataga gcgtccgggt acacagcttg ttcatgtaac cgctgtaata ctgcaaaacg
    26161 cgctctacgg ctccggcatc cccagcgcgg gcggcctcga tcaccgaaag gggcaaaagc
    26221 tcaatgccct tgtagttcgg gttcttcatc cgcgtctaac ctcccttcgg catcaacgcc
    26281 tttagctgtt tccgcagctc cgataaggta tttgtcctgt gccgctgaac ggcactccgg
    26341 gacattccca caaggccgcc gatctcacca tctgccatat ccagaacaca gtgcaggatt
    26401 aaaatactct gctccatgct gggcagcgcg gcaaaggctt cggcaacaag ctcattgttg
    26461 atatgtaggt catagccatg cgacgaaaac gtaaagctgt cgctggggta gtgatctact
    26521 gtacagagct tgtccaaatc tgcctgtgac aaagagctta gtgacacttc acggtcacgg
    26581 cggcgcttca tttcagccaa gtagcttttg gcctcgttac gcagtaccgt cttgcaaaaa
    26641 gcatcgaaca tgcaccgcgc ttcatagtcg cgggggatag catccatctt ctcaccccct
    26701 ttctgtgggg gacttaggtg gttctctccc tttccgctaa caagactttg acaaggccgc
    26761 cagctacccg ctaaatacct cctttttgaa aatttctcaa aaaatttttt tggggacagc
    26821 cgggccgcaa aagacgacaa aaatcgcccg gcaggtcgca aacctaccgg gcgagaaaat
    26881 gctttgctat gctgctgatt tacatggtat agtacatact cataaccgga atagccctgt
    26941 g
//
LOCUS       DACDYI010000041        26580 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.041, whole genome shotgun sequence.
ACCESSION   DACDYI010000041 DACDYI010000000
VERSION     DACDYI010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26580
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.041"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(19..729)
                     /locus_tag="IWM52_001634"
     CDS             complement(19..729)
                     /locus_tag="IWM52_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAP9973886.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(726..2198)
                     /locus_tag="IWM52_001635"
     CDS             complement(726..2198)
                     /locus_tag="IWM52_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9973887.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            2336..2809
                     /locus_tag="IWM52_001636"
     CDS             2336..2809
                     /locus_tag="IWM52_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9973888.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(2962..3513)
                     /locus_tag="IWM52_001637"
     CDS             complement(2962..3513)
                     /locus_tag="IWM52_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9973889.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            3618..4148
                     /locus_tag="IWM52_001638"
     CDS             3618..4148
                     /locus_tag="IWM52_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAP9973890.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(4631..5074)
                     /locus_tag="IWM52_001639"
     CDS             complement(4631..5074)
                     /locus_tag="IWM52_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9973891.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(5090..6250)
                     /locus_tag="IWM52_001640"
     CDS             complement(5090..6250)
                     /locus_tag="IWM52_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9973892.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            6757..8457
                     /locus_tag="IWM52_001641"
     CDS             6757..8457
                     /locus_tag="IWM52_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973893.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(8559..9392)
                     /locus_tag="IWM52_001642"
     CDS             complement(8559..9392)
                     /locus_tag="IWM52_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAP9973894.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(9696..11861)
                     /locus_tag="IWM52_001643"
     CDS             complement(9696..11861)
                     /locus_tag="IWM52_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9973895.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            12121..14415
                     /locus_tag="IWM52_001644"
     CDS             12121..14415
                     /locus_tag="IWM52_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP9973896.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            14408..15073
                     /gene="pgmB"
                     /locus_tag="IWM52_001645"
     CDS             14408..15073
                     /gene="pgmB"
                     /locus_tag="IWM52_001645"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP9973897.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            15066..16073
                     /locus_tag="IWM52_001646"
     CDS             15066..16073
                     /locus_tag="IWM52_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP9973898.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            16095..17114
                     /locus_tag="IWM52_001647"
     CDS             16095..17114
                     /locus_tag="IWM52_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9973899.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(17395..18762)
                     /gene="nhaC"
                     /locus_tag="IWM52_001648"
     CDS             complement(17395..18762)
                     /gene="nhaC"
                     /locus_tag="IWM52_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP9973900.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(18871..20292)
                     /locus_tag="IWM52_001649"
     CDS             complement(18871..20292)
                     /locus_tag="IWM52_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9973901.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(20535..22412)
                     /gene="tdc"
                     /locus_tag="IWM52_001650"
     CDS             complement(20535..22412)
                     /gene="tdc"
                     /locus_tag="IWM52_001650"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP9973902.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(22703..23959)
                     /locus_tag="IWM52_001651"
     CDS             complement(22703..23959)
                     /locus_tag="IWM52_001651"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAP9973903.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            24539..25840
                     /locus_tag="IWM52_001652"
     CDS             24539..25840
                     /locus_tag="IWM52_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP9973904.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(25909..26508)
                     /locus_tag="IWM52_001653"
     CDS             complement(25909..26508)
                     /locus_tag="IWM52_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9973905.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT         8065 a         5474 c         4762 g         8279 t
ORIGIN      
        1 tacatggtgt tcaaaatatt attcgccttt tgcttctttc actaattttg cgaatgcttg
       61 ttgttggaat tcagcagcta aggctttcgt acgttgttct tgctctgctt tgctcatttt
      121 tggattttca gtcattgctt ttaccatacc gtcttgaaca taagcttttg cttcactttc
      181 catcgtactt tgctgtttct tcactaattc agctaacttg gctgcttggt ccgtacttaa
      241 ttctacccat gtctctggga ttttgaatgt attcactcgg ctgtcatatt cgtgattgtt
      301 atcagcaata ttgaaccaga atttatacat gtcgtttttg tattcccaat aagtagtttt
      361 cgtcactttt tcagcatcgc cgtctgtctt ttctacttta tttgtttcgt tatccgtacc
      421 agttgctttt ggtttttctt gtttttcatt tgttcggtaa agatacactt tttcttttcc
      481 attccctaaa gctttataca aaagcatatc tgctccttgg gaatcagcac tggaaacaat
      541 tttttctgtt tttgtttccg ttactttttc cattccaaag tgattggaca aattagcgac
      601 gatcaaactt agtgaagcga cgaagattag tccgaaaatc aaagataaaa aggtttgcca
      661 tgttttttta gcgaacacgt tcgttaaagc aaaagcaagc aagctgacaa tcaaaactcc
      721 aataatcatg cattatcccc cgctttcatt agaacaggtt cttttttctt caataaaaag
      781 gcaataataa agccaagaat cccaaagatg atcgctacga taaacgcggc atggtaacct
      841 gacagtgtgg cattgattgc ctgatcttta taagctaatg gcgtattatc caatacagat
      901 ttgcttggga tatttgcttt tgtcacattc gtcaacacac tgatcaaaat agctgtacca
      961 atcgagcttg cgatttgacg gaatgtatta tttaccgctg tcccatgact gatcaagtta
     1021 tttggcaatg cattcatacc agaagtcgtt accggcatca tgaccatgga gatcccaaac
     1081 atacggatcg cataaaggat cacgatatat aaaactggag tcgtttttgt caagaaagca
     1141 aatggtgcag ttgccgctgt caaaatgaac atccctgtga tggctaaacg tttcgcgccg
     1201 tatttatcga aaatcgcacc cgtcacaggc atcatagccc ccatgatcaa tgcacccggc
     1261 aacaacatca agccagaatg gaatgctgat tctccacgaa tattttgaat atacaatggt
     1321 acgaccattt ctgcccctac catcgccata tttgttacac cagctaaaac agctgcaatc
     1381 gtgaatgtag atgatttgaa tacacgaagt tccaagaatg gatgttccaa atgtaattga
     1441 cgccatgaga acaaggcgat gaaaatcaca ccaacaacta agaaaccaat tactttcaag
     1501 cttccccagc cgtcatctcc tacactagaa aatccgtaaa gtaaactacc gaatccaatc
     1561 gttgataaaa tagcggataa aatatcaatg ctaggatttg acaatggaat aacacttttc
     1621 attaggaaga atgctaaaac caagacaaga acaacgattg gcagtaccat accaaacaag
     1681 tcacgccaat ggtagttatc aatgatataa ccagataatg tagggcctaa agcaggagca
     1741 agtccaataa cgatccctga catccccatt gctgatcctc ttttttctgg cgggaaaata
     1801 gatagcatga tattttgcaa taatggcatc gatactccca caccgcaagc ttggatcaaa
     1861 cgaccgatta gtaatgtact aaaattaggt gctgtaaagc aagtaatcgt tccgatcaaa
     1921 aagatcgtca tggcagaaag atataaattt cttgaactga atttattgat cagccatgct
     1981 gttattggga tcatgatccc gttgactagc aagaaaccag tcgttaacca ttgcacatca
     2041 gacgctgaaa tatcgaaagc tttcatcaat gtaggaaaag ctgtcgtcaa taaggtctga
     2101 ttcaaaacgg tacaaaatgt accaattaat aagacaacaa ctaaaagtgt ccgattatat
     2161 ggttttccgt aaatatctac gggttggttg ttactcatat ttctccttct ttctgaattt
     2221 tttctgaaac tactagaata ctttagtcct aaattcgcta tttgtcaacc tacttgacac
     2281 ataagttaat acggatataa ttaagggaag caagaggaca aaaggagtga caaaaatggt
     2341 tggggcttcc cttaaacgtt tgttggaaaa tgatcaattg gttgttggca tcagtgaatt
     2401 aagtgatatc gtaggtgtgt ctccacgtca attaaggtat tgggaacaaa agggattcat
     2461 tcgttcgatc gaagctgatg cgaattgtcc tagaaaatat cgtttaccta cagtcataaa
     2521 agtagaactg atcaaaaaat atttagatga cggatttact ttgtcaaaag ctgcagaaaa
     2581 ggcagaaatc cgccaaaaga aaatgcacca tgtccgtaaa gttttttcaa aagcaatcaa
     2641 agatatcgaa ttgatcgacg aacgctatac gattttacaa atcggtaact tcaaagaaga
     2701 gaatgctaag atgtacatca ttcatgacaa cgagacagac gaattaactt acaagctcct
     2761 tcctgtcgat gaaaaagctg actacgaaca gttgttaaaa gaactctaaa cgccactaat
     2821 ctcctaagta ccaagctttt taataagtag atgctacata aatacttgaa aaaaggcctg
     2881 tttgaacata agaatatccg aacggtgatc tgcgtttctt tcatatagaa aggaattctc
     2941 catcactgtt cggatatttt tttatttttt tcggttactt tcttctaata catctgttag
     3001 ccaatttgca tattctgttt cccgattgat cgcatgcgct aaaatcaggt agtgtccgta
     3061 attttcatgt ttttcttttt cagtaggaaa gatcgttttt tttcgctcta atagatgaag
     3121 tcgtttttct tcatgaaggt cacgctgctg ctgaataatt tctactaatg ccggatcatt
     3181 cacgtctttg acgaaataca atttcagaac aaattcgtct cgattaaccg gtagttcatt
     3241 ggtgggtgta tgaatccact ctgccaattg ttccttccct tcttctgtta attcataaac
     3301 tttcttttcc aatttttcac ctgtgatctc tacttgatgc tgaatgattc cttgctcttc
     3361 catttttgca agttcaggat aaatctgact atgttttgct tgccaaaatt ctcctatttc
     3421 atttttgaac gattgattca attcatatcc tgtcatcttc ttcttactta ataaccctaa
     3481 caaaatatat cgaagtgtat tttttcttgc catcagtttt ccctccttga caaatcttta
     3541 aatccattct atcataaatg agtaatcgaa aacatgtagt ttattacatg ttttaattta
     3601 taaggagatg cttaaacatg aaagaattta aagaattgga agattttatt ggaactcact
     3661 ttatctatac gtatgacaat ggttgggaat atgaatggta tgccaaaaat gaccatactg
     3721 ttgattaccg tatccatggc ggaatggtca aaggccgttg ggtaaaagac caagaagctg
     3781 atatcgttaa attgacggac ggcgtattca aaatcacttg gacagagcca acagggacag
     3841 acgtggcatt ggactttatg ccaaatgaaa agaaacttca cggaatgatc tttttcccta
     3901 aatgggttga agaacatcca gaaatcactg ttactttcca aaacgaacat atcgcagaaa
     3961 tggaagcagc tcgtgaaaaa tatgaaactt atccaaaatt acttgttcct gaatttgcta
     4021 cgatcactta tatgggtgat gcaggtatag acaatgaaga tgttattagc gaagaaccat
     4081 acgatggaat gccagatgat atccgtagcg gaaaatactt cgatgaaaat tataaacgag
     4141 taaaataagg ttgtgaaaga gaggttgtga aatcagcgtt ctgacctgag tattagagaa
     4201 agaaacggaa aaatagttcc tcatgttttg ccgttttttg agctaatacc gagggtcagc
     4261 tgattgaaca ccgtttatca agaggttgtg aaatcagcgt tatgctttga gtaataagaa
     4321 cgctagttag aaaatagctt ttcatatttt ctaggctagt gggcttatta ccgagaagca
     4381 gccgtttgaa caccgtttat caagaggttg tgaaatcagc gttttgacct gagtattaga
     4441 gaaacaaatg gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc
     4501 agctgattga acaccgttta tcaaagattg taacaactgc atattcacac agttaaatta
     4561 gtgacttcaa aatgaaataa tgctcccagc aaaaaattag gctgggagca ttatttcgtt
     4621 attctgttca tcaaaaatca gcgttttgat tccatttcat cacaagttcc tcctcattta
     4681 ctgcttcatc tttttgctgt gtttgaattg taaaaccttg tgaaagatag aaatgaatag
     4741 ctgattgatt tttttgatag actgtcaaag tcagtgtttt attggtttct tttatttttt
     4801 tcattagttg cgtaccgatt ctttgttgcc ggtattccct tttcacaaag attcctgcga
     4861 taaactgatc aatcacccca gcaaaaccaa taatttcttg atctatttct gctaagtaaa
     4921 tcactgcatc tggtaaagct ttcctaacag attctacttg attctcccaa taagttgatg
     4981 gaatgaattc atgagaatcc aagttagcat gcagccaaat cgatagaaca gattccagtt
     5041 ctttggcaga caattttgtt gttgtgcgga tcattttttc tctccttact tattcattga
     5101 ttttaaaata aaaaccattt ttttgaagct cttttacaag ttttcgcccc tcaaaaaagt
     5161 atatcttctc ttgttttact tttctaatac caaaagaaac agcaacttta ggatcatcag
     5221 ctgaaacgat tcgataagtt ccaccaggca attgatccag cttgactttc ccatgatggt
     5281 cgctttgtac tgaaaacggc acaccatttc tagtaagtgt ttttttctta tgtttcaaaa
     5341 tgaatgaact accatttacc ggtttccctg acgaatcact aataataaat gaaaaatcaa
     5401 gcactttttt acttaatcta tactgataga ttagtcgtcc gataaacact aacagtatga
     5461 ctacagctat tatcgttcct gcaagaataa accattgccg catggttctt tcctgatttc
     5521 cttttccttc ttcttttttt accgattcgt tataaggaat ccgatgtcct gtcactagca
     5581 aacgatgcgt gttgatcata tacggcgtac atgtaagcaa ggtaactaag tcttctcctt
     5641 ctgaaatcgt tagaacagac gtgtcatctg gcagtaccac ttgtatattt ttaacttgat
     5701 aagccaattt ctcccccaaa acagtcaaaa taaacgtgtc tccgttttct aaacgatcta
     5761 attcggtaaa taaccgtcga ttaggtaaac cgctgtgagc agagatcact gaatgcgtcg
     5821 aatttccacc aagcgggaaa gaggttcctt gcaagacgcc tgctccattt tcaaggatcc
     5881 taccattgag ccgatcataa agtgaaatat ttaattggat cttaggtata ttgatacttc
     5941 cgatcagcca ttcctctaat tggctggatg ttgtactttc atttctgttc ttttcattga
     6001 aaggatcagt accaggattg aatccttggt cagttaatcg ttggtttttc tcctccattt
     6061 tctttcgctg cacttcaatt tcagctgcgg tccgtttttg tacttgttcg atacgttttt
     6121 gatcaataaa actatttaaa gaatccacat aaaatggata taaagcgatc agtaacccta
     6181 cgacgataca tattaataaa aacaaacgaa acaaaagtcg acgagactgt ttttgcttac
     6241 tctgcttcac atgtgtcctc ccttgcaagt ttttttgttc tttcttttat tatatgggtt
     6301 caggaaaaaa atagaaaatg aaagccgatg aggttgtgaa aaagctgtcc tgcatcaagt
     6361 aataagtaca gcccaaaaaa atagctactc atattttttg ttgggttggg cttattaccg
     6421 cagatgcaag cttttgaaca ccgtttaagg aagaggttgt gaaaactgcg ttatgctttg
     6481 agtaataaga gcgctagtca gaaaatagct tctcatattt tctaggctag tgagcttatt
     6541 accgagaagc agctgattga acaccgttta ccgagaggtc atgaaaaagc tactttgttt
     6601 cgagtattaa gaaaaattct gtttcaaaac aaggagatgc aacaaaactt atgtcgcatc
     6661 ctctctcctt agaactttca cttatgtttt tcttgtaaaa ttcataaata ttttcatgaa
     6721 ttttctaatt tcttgcctac ctctcgcata ggatatatga tgaatttatc taaagtttgc
     6781 aaaggagttc gacaattgaa aaaatatggc ccatttttag caattttttt cattgctttg
     6841 gcatttctca tgcctcattt attcacacat aaactaattt tgggtgctga tgccccattc
     6901 cattacaatc ggttctatga tgccgcgcaa caaattaaat atggggattt ccattatttt
     6961 ccttcccttt atggattcca gcaaagtggc aggatcgtga atgccgttta cggcccattt
     7021 ttcgcttatt tacaaggatt gatcgtgttg cttgcaggta gctggtttca ttatcagatt
     7081 ctttcaaatt tcattcttta tcttatttca ggattttcta tgctgatttt actgagatat
     7141 gtcaatgttc gaaattgggt aagtgtacca gtagccgttt tattcatgag tacgtattct
     7201 attcaatact ggtctatcaa ccaaggattc actagctggg ggacttgttt ttatccttta
     7261 tgtatgatcc ctattgctga tttcgtcctt cataaaaagt tccctgttat caaagtcgct
     7321 gtctcagtgg gacttatgac acagatccat ttactgtctt cgatcatgtt agctttgatg
     7381 tatatccctt tttatcttta ttactttttt aaccagactg aaaaaggaaa agcactgctt
     7441 caactgatta agtcggtctt gctttatttt gttctgacag caaatatttg ggcaggatta
     7501 gctattgttt acagtgggaa taagattctt cctccctttg ttaacagaga aatgagtcaa
     7561 aaggcgatag atctggatgg cagttattgg ctccattacc ctaaggcctt tcctatattg
     7621 atattatcag gttttttgct ttttattttc tttcaacaaa agaacacctt attccaaaaa
     7681 atcgtgttgt acacaagtct tgttttcttt tttctttcta ctagcttgat tccatggacg
     7741 acattgatcg aaaaaaatgt gccttttatt agtttgatcc agtttccatt tcgatttttc
     7801 gtgccattta cgctattgct tctactttac cttgcccttt cactgaacca gtggtctgaa
     7861 caaaagtggt tccgtctttt aagtatattt ggcattctta ttttttcagc acagacgatc
     7921 cagaacttat accagcatct ggaaaagtgg gaaaatgaaa catttgtcag tcgacatact
     7981 tatctattcg atacgccgga agaagcaagg aaaacattct tcagcaaaga tatgtctacc
     8041 agcttgttcg ttttccaaaa aaccacacct gactatctgc caatctataa ggagacaaaa
     8101 gacaataaat acgaccggta taacgaagaa atactagaaa acgaaacaac ttttataaaa
     8161 acacatccag gcggtctttt gacaatcaag tggaaaaaca ctggggacaa aactgttcat
     8221 atccctgtta tcgtctacga tcggactgtg ctacagcaaa atggaaaaac attgactgat
     8281 tatgaagtaa cagatatcgg gaccccaatc gtgaagcagc aaaaaggaat caacgagctc
     8341 acgcttcatt atcaaacgcc aatctatttc tatttcattc tttctcttac tttgattggc
     8401 tggtttacct tgctatgttt atttatttat catagataca agcttttacg tgcttaggca
     8461 aaagggataa atacatccga tcaaaaaact ctccaaacgg tataatcgtt tggagagttt
     8521 tattttaatc agttttttta tgttatatgt tatatttttt attcgttcac atctaattcg
     8581 atttccaccc cgtagtgatc actgatgatt ggactatcga tcccatcaaa aatccgttga
     8641 tattttttaa cctgcattcc ttcagggacg aaaataaaat ctatacgcaa tttttctgta
     8701 ttttgttccc aaccatctat tttcttttcc acagtagctg cttttccttt ttcattcgcg
     8761 acaacatacg aatcttgaag aagaaggcaa ctttctctta cagtataata accttgagag
     8821 tctactggat tattgaaatc acctaggaaa aaaagtggta cgcgagtttc caaaaagtag
     8881 ttttccaagt ttttccactc aaaataaaag ccactttttt tatctgtcca ccaagaaaag
     8941 tggcagctcg agacaaaaat gtcctgatca tctatctttg ttttcccaat caagatcttg
     9001 cgtgtctgat aatcttcttt cttttgaaat tgggagacgg taaggacctg actttccaat
     9061 gtttttttcg taagcagtgc atttccttct tcatatttat cataaccgat gtggctcatt
     9121 tcccaactcc aaaaatactc ttttccgtgt tcctttaaaa gctgaactaa acggtaggca
     9181 aaattatctt tacggatagg tacttgattc tttaccggac agaacttcat tagcttcgca
     9241 ggttctactt cttcagattc cagtaattgg ttgatttctt gtaaagcaat cacttcataa
     9301 tcttctaaca ggatcttatc tgctaattct tgcaattttt tctctggttc tggctcaagc
     9361 cagctatgcg tgttgagtgt catgaatttc attggttttc tccttgtctt catttcgaaa
     9421 caaaagggtt gtgacaaaat ttttgtcaca acctctccag caaatagtgt tctcctgcta
     9481 actcctcagt aataagctca atggaccagg agctattttt tgtttggctg tttttatcac
     9541 ttggtgcagg gcagcttttt cacgacctct ccactaaaca gtattcaaac tgctgcttct
     9601 cggtaataag cccaaccaaa cgaaaaatag gaggagctat ttttgtttgg ctgttcttat
     9661 gacttgatgc aggacagctt tttcacaacc tctccttaca ttttaatgga gccgatcact
     9721 ttaccagctg tcactgtacc taattgcttg atcactactt cttctacttt atcgtttgta
     9781 aatacgatca tgatcgttgg atctttttct tcgttttgga tcacatcaag atccatctca
     9841 gctaatttcg ttcctgttcc tacttcttgg ccttctttga cagcaatctc aaaagctggc
     9901 tgattcattt ggactgtatc gattcccata tggatcaaaa cttcgatacc atctttcgtt
     9961 tccagcccaa ttgcatgttt gctagggaaa atacttgtca cttttccagc gatcggcgca
    10021 acaacttctc ttgatgttgg aataacagca aagccgtcac ccatcatttt ttgtgagaat
    10081 accggatctt ttacttcaga taaaggaatc acttctccag aagctgcgga aactagttct
    10141 tgttctatac ctaagtattg cttactacct gcttgtccat ttcctgtaga aaccatttct
    10201 ggctctggaa tctctgctcc tgattgcagc agatcttcga tatctgattt caagacatct
    10261 gcttttggtc cataaactgc ttggactcca ttgtctttga cgatcaatcc catcgcccct
    10321 gctcgtttcc aggcttcttc agtccctact ttttctcgat catttacact tacgcgtaac
    10381 cgagtcatac aagcatcgac atctttgata ttttcttttc ctcccagcaa atgaatgatc
    10441 tggaccactt gctgatctgc tgttcctgtt ccgctgttag atacaacggt ttcttcactg
    10501 tcattgtcgt aatttccgtt acgtcccggt gtagcaaagt tgaatttttt gatcaagaaa
    10561 tttgctaaga aatacgtaac aacaccaaaa gcgatcacac acaacacgaa gttgataaga
    10621 tctccaccta gacccgcttt gatagctaac ggtgtccgag tcagcaattc gatattccca
    10681 aatgaatgga cccgcaatgg caagatatct gccatcgcaa atgcagcccc ttggatcact
    10741 gaataaatga catataatgg cacagcagca aacataaaca tgaactccaa tggttctgtt
    10801 acacctgtta gaaagacagc caacgctgct gaaaaataca ttgatttata tcttgctttc
    10861 ttatcaggat cgacattacg atacatcgca aaagccattc ccatcaagat acctgaagaa
    10921 ccaatcattt gtcctacttt gaaacgagcc ggcgtccagt tatgcaacac aaactcatat
    10981 tgcgacatat cccctgcacc tttaagattc actaaatcag tggcccaagc caaccataac
    11041 ggatcttgcc caaatacttg tgtccccgcc tgtgcgcctg ataaaatctc atacgatccg
    11101 ccaagctgtg tatagttgat aggaatcgtc agcatatgat gcaatccaaa tggcaatagt
    11161 aaacgttcta atgttccaaa taaaaacggt gctagaatcg gtgcgctttc ttgtgattct
    11221 gcaatccata aaccaaaatt attgattcca gcctgtacgt ttggccacac cacagcaaga
    11281 atcaatgcca cgattgttga ccataagata acaacaaacg gaacaaagcg ctttccattg
    11341 aaaaatgaca aagcatccgg caattttcta taattatagt acttattata agccattgcc
    11401 ccgacaaatc cagcgatgat ccctacgaat actcccatat tcaaagctgg tgcttccaat
    11461 acacttgtaa aaaatccttt aaccatgatc ttcgtaccga acagagtgtg ggtaaatgca
    11521 tcttcattcg caagcatgtc acttgtgaca ccaaagatag ccccagtgat ccgattgatt
    11581 aaaataaatg agatcccagc tgcaaatgct ccgcctgctc tttcttttgc ccaacttcct
    11641 cctattgcta aagcaaataa aatatgaaga ttaccaataa ttgcccaacc aatattttca
    11701 attactccgc ccgttgtgac aagcggcgtc caatcaggat tgatcaaggg aattgatttc
    11761 cctatactga tcatcaaccc agctgccggc ataactgcta ccacaaccat taaagccttt
    11821 ccaaattttt gccaaaactc aaaactaaat atctttttca ttccgtttag ctcctttacg
    11881 caatcggttt cgttaaacgt attatagcgt gcgttttctt ttttgtaaat gctttatttt
    11941 ttatttttct gtctatacca ttctataaaa cctataatac gtgtgttccc ttctataatt
    12001 ttctcctaaa aatttatttt tttaagcttt tttctatgaa atattttctt ttgtctattg
    12061 acttgtgcaa tcggttgcgt ttattatgaa cgtataaaac agctcattag gaggagaaaa
    12121 atgaaacaga tcaaacgact atttgctatt gatccatgga aaatcagcac gacacaatta
    12181 gataaaaaaa atcttcgact gcaagaatct ttaacaagta ttggaaacgg ttatatgggg
    12241 atgcgcggga actttgaaga aacttactca ggggaccacc atcaaggtac ttatttggcg
    12301 ggtgtctggt atcccgataa gacacgtgtc ggctggtgga aaaatggtta tccagaatac
    12361 tttggaaaag tgatcaacgc cttgaacttt atcgcgatag atatttcgat caacgatcag
    12421 ctgatcgact tggcttcttt ggaaccagaa gacttttact gggaattaga tatgaaaaat
    12481 ggtgtattat ctcgtacatt cacaatcact acttctacta ataaagtacg catttccttc
    12541 gaacgttttc tgagcattgt caaaaaagaa tcagcttata tctatttaac tatagaaatg
    12601 cttgaaggta gcggaacagt caaactcgta tcaaaattgg atggaaatgt tcaaaacgaa
    12661 gacagcaact atgatgaaca tttttggaat gagattgatc gtggaactat agaagaaatc
    12721 ggctatttga cgactaaaac tattccaaac aactttggga ttgaacaatt tactgttact
    12781 gcagcaatgc gccatttcgt taatggaaaa tcagtcatcc ctgactatca cgaagatact
    12841 ttagcattac atggttgctt gtctgcagaa ttatctcaag gtgagacatg gtcagtggaa
    12901 aaagaaatta tcgttcttac tagtagagat attccagaag cgcagcaagc agaaaaagct
    12961 gtctcagaat taacgaacca gacaaacaat tatatccaag caaaagaaga gcaaacagcg
    13021 gcttgggcag aacgatggaa gcttgccgat gtggtcatcg aaggagacga agaagcacaa
    13081 caaggtattc gattcaattt gttccaacta ttctctactt actacggcga agatgaacga
    13141 ttgaatatcg gccccaaagg attcacagga gaaaaatacg gcggagccac ttactgggat
    13201 acagaagcgt atgctgtccc tctttatctt gctttagcca aacctgaagt aactaagaat
    13261 ctgctgaaat atcggcataa tcaactgccg caagcaatcc acaacgctca gcaacaagga
    13321 ttaaaaggcg cgctttatcc tatggtcacc ttcactggtg tcgaatgcca taacgagtgg
    13381 gaaatcacat tcgaggagat tcaccgaaat ggcgcgattg cctatgcaat ctacaattat
    13441 acgaattaca ccggcgatac ggaatatttg aaaaatgaag gactggaagt tttagcagaa
    13501 atcgcacgct tctgggcaga ccgtgtccat ttttcaaaac gagcaaataa atacatgatc
    13561 cacggtgtga ccggcccaaa cgaatatgag aacaatatca ataacaattg gtatacaaat
    13621 acgatcgcag cttgggtctt gcgttacacg cgagaaaatt atttaactta tcaaaaagaa
    13681 gctgctatca ctatcagcga agaagaacta gccaaatgga aagatatcgc agagaacatg
    13741 tacttcccag ttgacgaaga attaggaatc tttgtccagc atgacacatt cttagataaa
    13801 gatctcatgc ctgtatccga tattcccaca agcgatcttc ctctgaatca gcattggtct
    13861 tgggacaaaa tccttagatc ttgcttcatc aagcaggcag atgtactaca ggggatttat
    13921 ttcttcaatg atacttttac aaaagaagaa aagcaaaaaa actttgattt ctatgaacca
    13981 atgactgtcc atgagtcttc cctttcgcca agtatccatt cgattttagc agcagaatta
    14041 ggaatggaaa gcaaagctgt agaaatgtac cagcgaacag ctcgcttgga tttggataat
    14101 tacaataacg acacagaaga cggcttgcac atcacttcca tgactggtag ctggttgtcg
    14161 attgtccaag gatttgctca aatgaaaact gcccatgatc agttgagatt cgcaccattt
    14221 ctgccaaaaa cttggaaagc ttattctttc catatcaatt atcgcggacg tttattgttc
    14281 atctcagtca cagatgaaca agttactttc gagttattag aaggttctcc tctagaaatt
    14341 tacgtctatg aacagtctta ttctttagcg gatcgtctag aaataacgct aagaaaggag
    14401 gaagcaaatg tttaaagggg tattgtttga tttagacggt gtcattaccg atactgctga
    14461 attccattat cgtgcttggc gaaagcttgg tcaggaaatc ggcattacga tcgaccacac
    14521 attcaacgaa cagctaaaag gcgtcagccg agaagactca ctctctttgc ttttagctta
    14581 tggcggaaaa gaacactctt tttcaaagga agaatttgca gaattagcga aaagaaaaaa
    14641 cgattattat ttagagatga tccagacgat cgaaccaaaa gatgtcttcc ccggggtcgt
    14701 gcctttactg gattctttaa aagaagaaaa aatcaagatt gctcttgctt ctgcaagtaa
    14761 aaacgggcca ttcctacttg aaaaaatggg tcttacacct tactttgatg ctattgccga
    14821 cccggcagaa gcaacaaacg gaaaacctgc gccagatatc ttcctccttg cagcaaaagc
    14881 tgtcggacta aaagcagaag aatgtatcgg gatcgaagac gcgcaagccg gtatccaagc
    14941 catcctctca agtggcgctc aaccaattgg cgtgggacgt gctgaagacc ttggagagga
    15001 gatcccgctt gtccctgata cgacttttct tacgatcgat tacttgaaag aaaagtggca
    15061 tgaccatgga taatccagag atctccgtcg taccagttcc agagactgaa ataaagaaaa
    15121 tcacattgaa gaacgactac ttagaagtcg ttcttctcaa ttacggtgcc cgcctgcacc
    15181 aaatcttcgc accagacaaa gaaggaaaat cagaaaatgt tttattgtct tacgatcgtt
    15241 tcgaagatgt tctaacagac aaaagctttt ttggagcaac tgtcggacct gtagccggaa
    15301 ggatccggga tgcttcttgg ggtgagcata gattggagaa aaactgcgga tcacaccata
    15361 tccacggtgg cacgaaaggc tggtctttcc aattttggga agtagaagtt ttcaaaactc
    15421 catcatcaat aggtgtggtc ttttacttaa aagatgaatt ttctacctat ccaggaccaa
    15481 tcactgctac gatcacttac cgtttaacaa aaaacgaatt agagatgaca acctctgcta
    15541 gtagccttgt tgaaaccatc tgcaatccga ccaaccatgc ctacttcaac ctttcaggaa
    15601 atggaacaag agacatctat gaacatcagt taacggtctt tttagatggt gtgcttgaat
    15661 tagatcaaga aaaactgcct actggaaatt ggacaaaaaa agaaaaactg ccaatcgact
    15721 ttagaaaatc cccaacactg caagagatcc ttgcatgtta tccagatgga ctagatgatg
    15781 tatttctttt gcatcatcct aggctttcca aaacttcctt acaattattc gagaagcatt
    15841 ctggtcgcca aatgactata gcaacttcta ataaaagtat ggtattattt tctacgactg
    15901 gattcgaagc tgatttctct gtcaatggaa aacagatgca ttcaaactac ggattagcaa
    15961 tcgaaccgca agaaatccct gatatcgttc atttccccaa atttggatcg atcaacctcc
    16021 atcctggtca agaacggatc agtcagacga tttatcggtt ctctgcacaa taacaagaga
    16081 aaggtgtttt tatcatggct attaccgtaa aagatgtggc gaaaaaagcc ggtgttgcca
    16141 cctcgaccgt ctctcgtgtc atcaacgatc atcctagtat ttccgagaca acaaagaaga
    16201 aagtccgaaa aattatggat gaactaggtt atgttcctaa catgactgcc cgcaatctag
    16261 gaaaacgcat ttccagtgcc atcggcgtaa tcttaccccc tcttgattca aaagagcgga
    16321 tcggtaaccc attttatctt gagatcatgg aagcaatcaa cgaagaagcg cggaattatg
    16381 atatgactac agcaatcgct actgctaaaa attttgaatt gctgttagag aacgtccagc
    16441 gtatgcattt acaaaaacaa gtagatggtt ttattctagt gtattcagat agtcaagata
    16501 ctgtcattga ttatttatac caaaaccaca tccctttcac tttgatcgga cagccttatc
    16561 atcatgagaa cgaaatcgtt tatgtagata atgataacca gcttctaggt aaacaagcaa
    16621 cagaattttt gatccagaat ggacataaga atatcctgtt tgtgacaaac acgactcatg
    16681 aaagtttgta tttcgaacga tatttcggtt atcaaaaagc aatgatgatg gctggtcttc
    16741 ctgcgttatc cagtgtagta ctagagcatc cggaggatta tctgtccttt gagaaaacgt
    16801 tgaaagaaaa cgaagccaca gcattggtgg tcattgacga tatcttcgcc cttcgcacga
    16861 tgcagttggc gcaaatgtat ggctatgtca ttccagagac actctccgtc attagcttca
    16921 acaactcgat cttctcaaca ttaacacacc cttaccttac aagtatcgat atcgatatct
    16981 ccgaattagg acgtgtcgct atgcaaaagc tgcgcgaatt gatccatgaa gaattgagca
    17041 gtggcgtacg tttggtcatc cctcatcgct tgatcaaaag agagacagtc gttcctttag
    17101 aaaacccata ctaaatagct gactcactaa atacgctgac catttatcct atggtcagct
    17161 ttttatgtaa aagaagatac tacttctaac acattgttta tctgtaaaaa agagcgtggg
    17221 acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc ggaagcagaa
    17281 gcaactcctt cggaaataag ctgaaattca caaaaattta aaaagcaatt ttcttgagtt
    17341 tcttcttatt tcttggagtt aaacgcttct gtcccagcct cttttttcct tccttcaatt
    17401 ttttacacgt cgacgattca ataaaaaacc gcctaatatt gtaaagacag gtgcaagaat
    17461 cgagaaaaag gcgaatggca agtaatgcaa cgcgctaact ttcatcgttc ccataatgaa
    17521 tgtgccgctg actccccacg gtacgagaga attgacggtc gcacctgaat ccgctagtgt
    17581 tcgtgtcaaa taggtttttg ataagccaac gtcatcaaaa gaacgcttga atgtttctcc
    17641 tggaagaatg atggacagat actgttctcc aacgatcaga ttgattccga tacagctcaa
    17701 ggcagtaaac ccgactaaac gagaaggtcg gtccatcttt tcttttaatt cttgaacgat
    17761 ggtatcaacg atcagatatt tgatcagtaa cccacctaat gccaatgcaa gtaaaatcaa
    17821 actagcagaa cctaacatac tcatgatccc gcctcgagaa agcagttcat ctagctttgg
    17881 atcaggtgta tttgcgacat aaccattcat caagatgtct gctgcttttc cgaccgtcaa
    17941 acttttctca tggataaaag aaagaacgag agcagctgtc gatcccgcta gtaatgtagg
    18001 gatagccgga atttttttga ttgccccccc aaataaaata actagtggaa tcaatgtcca
    18061 aggagagatc caaaagctcg ctttcatgtt ttggatcatt tcattgatac tagctagatc
    18121 ggctgaatga ctgttatgtc ctaatatcgt ataaaaaatc aacgaaagga cgaatgctgg
    18181 gaaatccgta taacgcatac cagcgatatg ttcgaataga ttaactcctc caatacccgc
    18241 tgccaagttg gtcgtatctg ataaaggcga gcagttattc cctaaaaagg caccggaaac
    18301 gactgcgcct gtagtcattg catcattgaa tccaagtaaa tgaccgatcc ccaaaaaagc
    18361 aattccaatc gtcgaaatcg ttgtaaatga actgccgaca gtcactccga caattgcaca
    18421 aacaagaaat gctgtcggta aaaagaaacg cacagaaaca agtttaaatc cataaaccat
    18481 aatcgtaggg atggttccag ataaaatcca tgaagcaact aagacaccaa tcaaaagaaa
    18541 aatcaggatt ggaatgatcc ctggagtgat tcctttgatg atcccgtcat ggatctcatc
    18601 ccatgtgaac tttttaaatc tgccataaaa caacaaacac atgaaagctg caaggatcgg
    18661 gatatgtggc gagagctgga atccgatgat cagtactcct aggagtgcca acaacacgat
    18721 caatacagcg atactttctt ttaaagaaat atttttattc atcgtcgatc tcctatttca
    18781 aatgaaaagg ccctgccatg aaaaaactat tcacttggca ggacctccta ctaaatcagc
    18841 gacaaaacgt cggctgaaag acttattttt ttatttttct tctaaacttt tttctacctc
    18901 aatcccccaa gaatctttct tgaagtgatt gatgatcaat ggaataacga acatgaccac
    18961 tgtgatagct gctactaata atgcatcact caatgtcgct gtacctaatg tagcaaatac
    19021 gacaacgatg atagagaaga tcagcatgca gctgactacc caagctaatc cgtttccgtt
    19081 tttaccaatt cggtaaggac gttcgaattc tggatgtttc ttacgcattt taagcaatga
    19141 aacagcaatc aaaatgtaca cgatacaata aataacagtt gtagcatttg taagtgtcaa
    19201 gaatacagca cttacgtcat ccattacacc ataaagcaaa gcaaataatg aaataacgat
    19261 tgattgtgtt agaacgacat ttctagatac gccgtatttg ttttctttat ggaatccgaa
    19321 ttttggtgga aggaatcctt cacgagctac ttggatgatt gtttttgatg gtccagtaac
    19381 ccaagctgat aattgtaaaa gcacaccaat aaataccatg aagctgaaga tatttccgat
    19441 cacttctggt aagcctagga tttgacagtc aatcaagata ggctgcgtga tatttgctaa
    19501 ttccattttt ccatctggta cgacatttgc tacaagcatc gcattgatga cattcaataa
    19561 aactaatccg atcaaagcga taaatacgcc ttttgtataa ttttttgttg cgtctttcaa
    19621 acgaggcatg taaacagaac tcatttcgat cccaacaaaa ataaatgtga taccagctaa
    19681 gtatttcaat gaatcaatat gttccaagtc agggaaagct tttgaccatg aaaagtcacc
    19741 tagatagccg tttgagacta gtccgacttt gaaggctgcc aaaacaccta aaacgaacat
    19801 gatgactact gggatatata cccctagcca tacaccgatg ttcccgccga cttttgccat
    19861 atcgaatttc aagttcaaga tcgtgatgat ccagtagata actaaaatac aacctaaaac
    19921 gaaccagtga ttattgccaa gttctacatt accaaatgtg tttcctaaca atggtcctaa
    19981 agtagaagcg accattacca tacctgggaa catttgcacc cataatagcc atgcaacgac
    20041 aaatccccat ttgctgccca gtgctgtttt tacccataac tgaggaccgc cttcttgagg
    20101 aagcatcgtg gaaagctcac cggaaaccat tgatatcgga ccggcaaaaa acaaaactgc
    20161 aaaaattgaa taaaaaatca atgtccaacc gactgcggct aacgtaggaa tactccgaac
    20221 cgttgcacac agtgccattg tcagaccgat aaaggtccca agacccattt tacttacttc
    20281 agatttactc accgttgaca ctctccttga ttttttggat aaaataacgg tgtctttgaa
    20341 gtagtttcag caataaacga aacaattcag cgaatatttg aggggattct ttgagtttat
    20401 tgcttgaagg gtttcttcaa agacaacctc aaaacaaagg gatcgaagag cttaggtctc
    20461 cccttgctct tcagatccaa acatgcgtca gaaacagtta gtattcaagc tgttttctaa
    20521 catgattacc ttgcttattt tgcttcgctt gccaataatt ggtctgcgta gattttttcc
    20581 aatttttctt gaagagctgc tttgattttt tcagcatatt cttcaaagtg ttcttctttg
    20641 ttcatgtatg gtgtcattac gctggcacgc aatacagtta cttttccggc acggttccat
    20701 tcttcatctg agaatcctag ttggttcaca aattgtaatg ggctgttgcc atagtctggg
    20761 atagcaaagt cagtatgtga agttaagaac tcattaccgt aaatgcttcc tttcacgtat
    20821 gaagaataat catatacgtc atggttcaat ttattcattg caactaagtc atcgttgcct
    20881 ttttctttga atacatagtc taccatattg aaatctggat aagttaatgg atgaacttcg
    20941 atttctttgt ctcctacttt aaatgacaag tcattcaaga agttgtagaa acgatgtgca
    21001 ccttcgattg atgcacccat taatttacca tatcctgtga cattcaaagg taagacatgg
    21061 tgtgctgccc aaacgctggc tgctgttgcc cctgcttttg aaccttccaa gatataagcc
    21121 ccaagtaatg ctgggatatc tgcgcctttt tcaaatacat atgtcgcaaa gtaagagata
    21181 acgtctctca tgcggatatc tttgatgacg ataccaccgg cagaatatgg aacatagccc
    21241 attttatgtg ggtcaatcgt tacagattct gcttcttcga ttgctttata tgcactgtgt
    21301 acttcttcta agatataatc tttgttttct gtaaagacat tgtatttgta atgaacatct
    21361 tttaaatctt caaatggaat gaagttgttg tcttcgtcta agaaaatcgc acggccgtat
    21421 ccgccataag cagcatctac gtgtaagtag aagtaaatac cgtctttttc taatacgcga
    21481 cgcaatgcaa cgattttatc gataccgtcg attgctcctt cttctgttga tcctacaaca
    21541 ccgactacac ctaggattgg tgttttttca gctgctagac cacgaacgat tttttcaagt
    21601 tcgttgatgt ccatgcgata gttgtggtca actggcactg gaatcacttg gtctaaaccg
    21661 ataccgatga tgtcggcagc ttttagccat gaatagtgtt tagtttgtgg aaccaaccat
    21721 tttcctaatt tttctaaatg ttttccgcta cgtgctgagt gagctttgat ttcgtcgatt
    21781 ttttctggga cgctgtctaa cagattcatg atttcttctg ttgacaagtt cattaattcc
    21841 caatcgcttt ttccagcaac tagttcaggt gtcacttctt tcattgcaag cggcaatgat
    21901 ttgatgttgc gggcatacca aagtccttct aagtttgcta aagaaccatc agcaacgatg
    21961 tggccccagc catctttata gctcattaat tttgcaaatt ccattcctac ttcttcttcc
    22021 atttggcttg ttgcaggaga agattcatag gcaacgttat taccattcca tagcatagca
    22081 aaattgtacg ctagtaaaga tggcatcaat gtttcagagt tcatatgacc ccagtaacgt
    22141 ccggcattat gccaaggaac ggaatgggta cgcatccgcg ctgaaatttc tgatagaaca
    22201 tcctttgttt tattcacagt gtgttcaaaa ctagcactgc ttttttcttc tggcgtaata
    22261 ataggcatgt cttgaggcat gtagttttga cgccagccta aatgttcatc caccaattca
    22321 ttcaacaaag ctttatagat ttggccgttc tcagctttgt ccccaatgaa tagggcattt
    22381 aggtttagat ctttcgacaa tgattcactc atagtgaaac cctcctaaaa aaatatttaa
    22441 attttgtcga gccctacatt acacctgttc ttctattctg taaactaagt tttggcctga
    22501 tttttaatca tattttaata tgtatcaatt atccgaattg ttcacacttt tgaaataatt
    22561 aaaaaaaacg agggaattta caaatgtaaa ctccctcgca aagattgtat taacgacaac
    22621 cagcgttcta ttttaggatg ggtttcaaaa ttggaatgtt gtaatagtat ttcccttttt
    22681 taaggtaaaa acttactaaa ttctaatcaa ttactttggc caaaaagtaa tttttcttac
    22741 cttttcgaat aaccacaaac ttaccatcga attcatcgga tggatttatg acaaaattta
    22801 aatcggtaac gcggtcaccg ttaatactta tagctccgtt tgaaacatct tcacgtgctt
    22861 gacgtttaga tggttcaatt tttgtggaaa caagcagttc cacgatattt tcaggtgtgc
    22921 ttgaaatttc aacgttcggc attttaccga atccttgcgc gatttcttcc gcatttagtt
    22981 ctttaatatt tccagagaac aatgcttgcg ttattttttg cgcttctttc aagtcttctt
    23041 cactatgcac aaatcttgta acttcttctg ccaaacggcg ttgtgcttca cgtttctcag
    23101 gttctgtctc tacttttttc gctaagtctt cgatttcttc ttgagacaag aaagtaaaga
    23161 atttcaagta tttcactaca tcgcgatcat cttgattcaa ccagaattgg tagaactcaa
    23221 atggtgaagt tttcttcgga tcaagccaaa cggcaccgcc tgcagttttt ccgaatttcg
    23281 ttccgtcagc cttcagcatc aatgggatcg ttaaaccgaa cgcttctgct tctggtcctt
    23341 ctaattttcg gattaggtct aatccagctg tgatatttcc ccattggtct gctccaccga
    23401 tttgcaattg cacatcgtat gttttatgca gatgcaaaaa gtcgatagat tgcaggattt
    23461 ggtaagtaaa ctctgtaaat gagattccta cttccaaacg gctggcaacg atatcttttg
    23521 ctaacatcgt gttgatgttg aagtttttac cgtagtctct caaaaattca agtaaagaga
    23581 tttttgacaa ccagtcgtag ttgttcacaa atgtgatatt tgcatcttta ccaaacaatt
    23641 ttctcatttg attggaaagt gcgtccacgt tatgttgcac ttgttccatc gtttgcaaca
    23701 cacgttctgt tttgcggccg cttgggtcgc cgatcgttcc tgttccgccg ccaatcagaa
    23761 tatatggatg atggcctgcc aattggaatc ttttcatcat cataaatgga atcaaatgtc
    23821 cgatatgcat cgaatcacca gttggatcaa cgccgcaata aagtgaaatg ctcttttctt
    23881 caacgagctc gcgaagtctt tcttcgtttg tttgctgatt aatggcgtcg cgccaagtta
    23941 attcatcaat aatattcatt ttactgacct ccgtgtagtt tttttatgaa agcaggaacc
    24001 tgctagcata tgtaattaat tatttcttca ggataggata ggaaataaaa aaagtccctt
    24061 ggcatataag ccaagggacg aaatctcgcg gtaccaccca atttgtatgt ctagatatcc
    24121 aacatacctc tcgaacattt aacgctgtcg cgtggcaatc actgccaaag actcctgaaa
    24181 tgtaattcgc tttaaagcat gtactggttt gcagcgacca ccagctttct gaaacattgg
    24241 ctttaacaac tacttcgttt cgatcttcgt cttgttatta ttaatatgtg tctatcatag
    24301 agcgcaactt tttctttgtc aacagaaaaa ggatattttt tttaaattcc tcattttttc
    24361 ttttttgaat ccgctctaat tccgaacatt ttattttcaa ttattgttaa aagaaataac
    24421 tttttctgta ttttttctgc aatttatcga gaagtcactt tactttgcct cttattcttt
    24481 cttcgttaca ataaggaatg ggacaagtaa aaaaatgaaa aagcaaggga cgaggtatat
    24541 ggaaaaaaag caaaaaatta ttttaggagc cacttctgct ttaactatcg ttattttaat
    24601 tctaagtttg ttttttgtca tccgatcatc tacaaaaaaa gaaccaatca ttcctgaaaa
    24661 aaaaataaca gaaaaagcaa aagaaccgac agatgataag caacaactag ttactcaagc
    24721 aaaaaatctg gctcaaagct acgattatga ccaagcgatt gcgttattgg aaaaagatag
    24781 taagaacgga tctactgaaa cacaacagct tcttcaaaca ctaaaaaagg agaaagaaca
    24841 actgatcgac tggtctgatc ctactcagat ctctcatgtc tttttccaca gcttgatcgt
    24901 ggacccgcaa aaagccttcc actctcaaca ggcacaagga tacaaagatt atatggtgac
    24961 tgttgaagaa ttcaaccgtt cgatcgaaca actttatcaa aatgattttg ttttagttaa
    25021 tttgaatgat ttgatccaaa aagacgaaca aggaaatctt tcctttaacg ggcttgcgct
    25081 acctgaagga aaaaaaccat tggttctttc acaagatgat gtcagctatt atgaatatat
    25141 ggataattcc ggttttccta gccgattggt cgtatcaaat aacaatgaaa tcaaaaatgt
    25201 ctataaagag aaagataaag aagaaatcgg tgactttgac atggttcctc tgatcgatgc
    25261 ctttgtcaag aaacatcctg atttttctta tcgaggcgca aaaggtacct tggccctgac
    25321 aggttacaac ggagttttag gttatcgaac atcaaaatct caatatggag acaatgaaaa
    25381 aacaaaaaaa gagattaaag aagctaaaaa agtggcggaa cagttgaaga aagacggctg
    25441 gagctttgct tcccatacat ggggtcactt gaatatgact caagcttcac tagaagatgt
    25501 aaaaaaagac aatgaattgt ggcagcaaga agttgcacct atcctaggaa aaacaaatat
    25561 tctgatttat ccatttggtg cagacatcag cgactggcag ccttattcag cagaaaatca
    25621 aaaatttgct tacttgaaga gtcaaggttt cgatattttc tgtaatgtcg atgcttctac
    25681 accagcttgg ggacaattag gtacggattt ctatcgaaat gctcggatca atatcgatgg
    25741 cattcgtttt caatctgatt tagacggaaa aaatcctatt cttgatccat tcatcaatgt
    25801 ccatgatgtc tatgacaaag aagcacgcga atctgcttga ttttacgtca tttatgaacc
    25861 aaaaaagccg aactgacatt ttatcgtcag tttcggcttt tttatgcttt atttcttctt
    25921 cggtgcgatt cccttgatca ggacttctgt ccattctgct gtatatggaa gcagttttgc
    25981 gtatatctcc ggcttagaaa ttccttctac cattgcttga aaatagagga tcaagaactc
    26041 atccgaatat ttcaaatcca cttttccttc tttccgccct cgatggaaaa gatcaagcat
    26101 gatactgaaa ctttcttgcg tatacgcatc catcgcgtca gaaaattctt ttttttcttt
    26161 ttttgtatag tatgtcatca aatcgatata aaattgtttg ttcactcgat tgagataacg
    26221 gattttgttt tcactcatgg caatcagtgt ttcttcaaat ggaagatttt ccgcaatcaa
    26281 cgctttcgct tcttgcccta aatcattcat aaatgcaaaa aatacttcat aaatcagccc
    26341 ttccttgctg ccaaaatatt taaaaatcgt cgctttactt acctgagcgt tctcagctac
    26401 cttatccatt gtcactccag cggttcctat gtctgtgttc atcaagtcaa aagctgcttg
    26461 taaaatctct ttctttttct cttgtgctct cttttcaaaa ccgttcattt gtagagcctc
    26521 ccgtaatttt aaacttttat aatatgtata gttcaaattt attctgaggt ttattataac
//
LOCUS       DACDYI010000042        26553 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.042, whole genome shotgun sequence.
ACCESSION   DACDYI010000042 DACDYI010000000
VERSION     DACDYI010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.042"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(99..1229)
                     /locus_tag="IWM52_001654"
     CDS             complement(99..1229)
                     /locus_tag="IWM52_001654"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAP9973906.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1878..3080)
                     /locus_tag="IWM52_001655"
     CDS             complement(1878..3080)
                     /locus_tag="IWM52_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9973907.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="IWM52_001656"
     CDS             complement(3140..3343)
                     /locus_tag="IWM52_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP9973908.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="IWM52_001657"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="IWM52_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4880..5692
                     /locus_tag="IWM52_001658"
     CDS             4880..5692
                     /locus_tag="IWM52_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9973909.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6716..6868)
                     /locus_tag="IWM52_001659"
     CDS             complement(6716..6868)
                     /locus_tag="IWM52_001659"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973910.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(7059..7937)
                     /locus_tag="IWM52_001660"
     CDS             complement(7059..7937)
                     /locus_tag="IWM52_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9973911.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7976..9094)
                     /locus_tag="IWM52_001661"
     CDS             complement(7976..9094)
                     /locus_tag="IWM52_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP9973912.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9091..11190)
                     /locus_tag="IWM52_001662"
     CDS             complement(9091..11190)
                     /locus_tag="IWM52_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP9973913.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11241..12062)
                     /locus_tag="IWM52_001663"
     CDS             complement(11241..12062)
                     /locus_tag="IWM52_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9973914.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12059..12868)
                     /locus_tag="IWM52_001664"
     CDS             complement(12059..12868)
                     /locus_tag="IWM52_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9973915.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12889..13383)
                     /locus_tag="IWM52_001665"
     CDS             complement(12889..13383)
                     /locus_tag="IWM52_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9973916.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13395..13883)
                     /locus_tag="IWM52_001666"
     CDS             complement(13395..13883)
                     /locus_tag="IWM52_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9973917.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(13960..16656)
                     /locus_tag="IWM52_001667"
     CDS             complement(13960..16656)
                     /locus_tag="IWM52_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9973918.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(17084..18157)
                     /locus_tag="IWM52_001668"
     CDS             complement(17084..18157)
                     /locus_tag="IWM52_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9973919.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(18154..18981)
                     /locus_tag="IWM52_001669"
     CDS             complement(18154..18981)
                     /locus_tag="IWM52_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9973920.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(18982..19788)
                     /locus_tag="IWM52_001670"
     CDS             complement(18982..19788)
                     /locus_tag="IWM52_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9973921.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(19800..20885)
                     /locus_tag="IWM52_001671"
     CDS             complement(19800..20885)
                     /locus_tag="IWM52_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9973922.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(20902..21444)
                     /locus_tag="IWM52_001672"
     CDS             complement(20902..21444)
                     /locus_tag="IWM52_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9973923.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            21702..22691
                     /locus_tag="IWM52_001673"
     CDS             21702..22691
                     /locus_tag="IWM52_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP9973924.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            22688..23425
                     /locus_tag="IWM52_001674"
     CDS             22688..23425
                     /locus_tag="IWM52_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAP9973925.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            23422..23973
                     /gene="coaC"
                     /locus_tag="IWM52_001675"
     CDS             23422..23973
                     /gene="coaC"
                     /locus_tag="IWM52_001675"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAP9973926.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            23989..24621
                     /locus_tag="IWM52_001676"
     CDS             23989..24621
                     /locus_tag="IWM52_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9973927.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            24706..25353
                     /locus_tag="IWM52_001677"
     CDS             24706..25353
                     /locus_tag="IWM52_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9973928.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            25358..26341
                     /locus_tag="IWM52_001678"
     CDS             25358..26341
                     /locus_tag="IWM52_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAP9973929.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
BASE COUNT         8120 a         5607 c         4503 g         8323 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggtaatata actacctgag tttattatcg attaaaatgc taactacatt
     6541 tgcaagagaa gcgagagtta aaggtaaata tagaatttct acttttttag caatagaaat
     6601 aggaataaaa tatttaaaat gtatagaaac tttctttttt tttcttgcta tctttttata
     6661 atcacaactc tctataatag aattacgatt tattttatac ggctgaaaaa atctatcacc
     6721 aaaaaaattc aaacattaag ccaaggatta tgaagtcaac atttcgaaaa atgaacatac
     6781 attggtaagc aactataact attaccacag acataaagta acacaatata ttactattta
     6841 agtgtacgcc accagtgaat atcctcaagg tatacagcgc acaacaaaaa gaaaaaagga
     6901 aacgcagaag catttccttt cagtgattta atgaaaacaa cacgatttga cttagagcct
     6961 ttttatttga attcattttc gctttatcca acttcatttg atcatttgat tactcggtta
     7021 aatacttttg acatgagtat caatgaaaca ttcattaatt atggttgctt atttcttacc
     7081 gcttttctgg ccaatgctaa tccgccgatt attccggcat tatcacctaa ttcgggcgta
     7141 ataatgtagc tatcaattgg tggtatttca acatatccat ttataagctc aacaaattta
     7201 tcctgaactt ttttcttcat gtgttcttgt ttcattacac caccgccaaa aataattcta
     7261 tctggcgaaa acatcagtgt tgtattgtat gcacattggg caaggtaaaa ggcttcaatc
     7321 tgccaaaacg aatcgtcagc caataaattc tggcctttta cacccaaccg tttttcaatc
     7381 gctggtcctg ctgccatccc ctctaaacaa tcttggtgga agggacagtt tcctgaaaaa
     7441 ttgtcctttg gatgtcgctt gatcaacata tgccccattt ccggatgact gaacccttcc
     7501 acgaaagtcc cattgaacaa ggcaccacct ccgatacctg ttcccacagt gaagtatgca
     7561 acacctgcta accctttgcc agaaccagca acatattcac catagcaagc cgcattgaca
     7621 tctgttgtcc attcaatcgg aatattgaaa tattgcttta gcgttcctac aaaattgaaa
     7681 ttttgccaag caagttttgg tgttgatgtg atatgaccga aactcgttga tttgcattga
     7741 atatcaattg gaccaaagca gccgaccccg atgcctgcca attgttcctt aaaaggttta
     7801 aaaaaatcaa tgacctgctt cattgtttca actggagttg ttgtgggaaa actgactctt
     7861 tccaaaatcg ttaattcctc agtaccgact gcacagacaa atttagtccc tccagcttca
     7921 attgccccat atatcatcat aggttcctcc cttcgaatat gtgaaaaaca cttcattaac
     7981 gcggcaagcg ttctcgatca gtgaaatgac gtttagtatt tcctgaagct tctgttgaat
     8041 accagtaagc gacactagca acatcgtctt gccgctcaaa taagcgaaca tcatcgttgc
     8101 cgatttgttg taaagtgacc gataaatctt cggtaaaaca gatggggtct tgaagatgcc
     8161 aacgataaag accatgtcgt ggcattgcgt cgtcaccaaa ggcatgaacg gcattcaatt
     8221 ttcccgtttc aaaacgatct cttgtggcat ctctatttga ttggtaagga taccctaaaa
     8281 aggctgttga atagtttttc gcatgtggac gtccgaactc atcgtactca tgaaaggccc
     8341 acgccccgcc aaaataatct tctgaacctg ttgaggttac ggtcggatac tcactatccc
     8401 catcaatgta gaatttaaac tcgccttcgc cccaccaata acgttctagg caggtaagcg
     8461 caaggtaagt acccacgtaa tagccttgac cctttaatcg atcaattaac ttataatcct
     8521 cctgtaattt ggtaatgcgt tcccgatgcc aatttgcgtg aaaatatagg ctattttctt
     8581 ccaattcgtc caccaaagcg taatttatcg tataaaaaaa tgaggacata tccttcggat
     8641 gtaaattctc gatcgtaatt ttagcggatg tttggaaagg catttcgaaa taggaattaa
     8701 agccccctgt aggattaacc actatcggca acgagttcac ctcgcaacgc tctccaaacc
     8761 cattacaaaa gaaatcccct aaaggacttt caacagcagg gctatccgac ccatcccaat
     8821 aaattcttaa gattaaatca cgtaaaacaa aactgccaaa ttctgttcga tcacgaatcg
     8881 tcatccacat atgacgaatt attcccgttc cagtaatatc cgcaatcaca gtttcttcac
     8941 cagaagtcag cggaattgcg ccacgacctt tgcggcttgg tcctagctcg cttggcaaca
     9001 tcgcagcttt acctttctcg cctgttggat tttcgggagt gattgctcga ctctggattt
     9061 ttttaaattg ggtaatattt tttagtatac tcatcttttt ctcctttgaa tgtgaatcgt
     9121 ttttcccttt tctacgtgaa gaattttttt cgtatcactt attttcaatg gcttttctga
     9181 caaatttgaa atactcaact cggattgcgt acattcaatc gaaatgaagg cttgaccgaa
     9241 cttcaaattc ttccaagaaa acgttaaatt atcaacagga tcaatttgca atactccttt
     9301 tttgaatttc aatccgaaat attgactgat cattaggctg acaaaaagac ccgatgccca
     9361 gccgcatttg ccgcagccaa agtctcgcac gacttctcct tgttgagcct ctaagcgata
     9421 cggccaccac caccacgaac cgtctaaatc tgccaaacaa attagttctt ctaatcgggt
     9481 tacgaaagct tgagggttgt gcagctcgct cattaaaaca gtgataaagc caggaaaagt
     9541 ggcgccggat tctaatcccc aagtgatgcc tttgatttcc tcgctatagg ttggattgaa
     9601 agaactagca gaaaaggcca tggtgttctg atataaatga tctttattat ctaaaaattg
     9661 ataaaaaggc atcaacgtag tgtctgattc ttctccatca tgcatcaata aattaatttt
     9721 tccatccaca atcacatcac ttaaaaagat taacccttgc tcaagaatcg gtttttgata
     9781 gtgcttcact gaataggttg ttttctcctc atcaccgatt ccctccaaaa attgctcacc
     9841 aaaaggacca tcgatagtca tttgtttaca aattgattcc tttagtttac aggcaatgtt
     9901 tttgtatcgc ttgctaagac tcagttgttc tagggcttca aaaatttctg caagtccaat
     9961 acaggccttc caaagacaaa gattgctgcc ggtatgatac tttccaagtg caaaggcgtc
    10021 tgataaccaa acagatggaa aaagcattgg ttcccctgac cgacgttgag ctaatatgtc
    10081 ctcaataatt cttgaagcat tcagtagaac ctgcggacac cgctcaaaga attttgtatc
    10141 cccagtatgt tcaaaataaa gactagccag taacgcactg gataacgaat tactcagaga
    10201 atgattgata cctcctgtaa attttgaacc aggaaactta acgccatatt gatcaaacca
    10261 tagaaccgtt ttttgacata actccggttc aaacatggtg aatggcaatg atgcgtaata
    10321 catatcctta ttccaaattc gattagtagc aggatagctg ccccaattgc tgccgacgac
    10381 ctgtccctgt cgattcatac cgaaactaga aaagctttga taaagtgcac gacgatagag
    10441 atggacaact ctatcgtttg gcattgacag ctgcccaaat agctgagcaa aatagttcaa
    10501 tgtttccttc agccattttt cgatatcaga ttcctgaaaa gtcattactt cttcaaaagc
    10561 atttgggtcc acaaaagcaa tggcgaagct tgccacttcc tgttgtgcga tcgtgtcctt
    10621 atttcctttt tgagaaatca acacattttg ctgatccgaa tacttctctt gatacgaagg
    10681 cacatctttc actgaaactt gaaggctttc gtgtgtttca ttcacaatgt aaacctgttg
    10741 aacaagcata cttaaacgct tattagccag tatgggagca aaggatacta caatgacctt
    10801 aaacgtcgaa taatgatgaa tatatctagg caaacagtct tgaatcacat cgacttcaat
    10861 acgatgatcc gattctgaca aatggtagac aatcccgttt acttcaacct ttaaggataa
    10921 cgaattggtt tgagagagct gtttattgac ccaaacacct ggtttttcct cagttagtaa
    10981 attttctcga taaaaggtca acgtttgaat cgtcccgcaa acatcaaggt ttgctaatgt
    11041 cttttgatta cttacatcga aggtcaatcc ctttacatca ggaatattcc gatagtagct
    11101 gtattcgttt gaagtagtca catttccgtc gtttaactca gcattaaaaa atgggtgttt
    11161 gggtgaataa attacttcat taatagtcat cttttccctc cttgctaaaa aaactgcagc
    11221 ctagtagctg cagcttccta ctatgcgatt ccaagtaatg acaataaaat tcccaagacg
    11281 accattccaa aaataatttt gatgacgccg acttttttat ctagtaattt gaaacagctc
    11341 aatgttacca ataacggtac aattccttta aaaatactat cgaggatttc ttggacattc
    11401 attagttctt cgcccttcat tgaaaagctc aaaacggtat tgaatttgac catactgatc
    11461 gtcattgcac ctaccatgat cagacccaag atactagcag cttttgtcag aatttcaatt
    11521 aacccgttcg catacagctt ttcaatatat ttagagccta atgaatagcc taaaacgccg
    11581 ccatagtatc gcatcaagta attaggtata ttgaacataa ctaaaaagat aattggtgcc
    11641 aaaatatttc cggcattggc taagttgatc gctagtcccg cggtaacgac acgccaaact
    11701 ccccagaaca atgaatcgcc aattccagcc aatggtccca tcaaactcgt tttgatcgcg
    11761 ttgatacttt ccacgtcaaa accagctttt tcagaatttt ccttctccat cgatgccgca
    11821 atccccatga caaaggacgc gcaaggaatg gtcgtcgaaa aatactgtgt atgacgttcc
    11881 agcgccgcag ctcgatcttc ttcacttttg tagaaatgat taatgaaggg catcatagaa
    11941 taggcgaaac caccgccgcc catcgtcacc ggattcaccg aagcatacat agtcatcgag
    12001 cgccaaaaca tcttccgaac caattttttc tcatctttag aaattccatc agctaatttc
    12061 attgaaagaa atcctcctct tcttggtcaa gcgttgcatt tacagcagca tcgctttgaa
    12121 ctgcatgaac agatatattt tttagttgta gatctctttg ggctataaca attgcaataa
    12181 tcgttccaac tgcggcaatt gcaatcaatg gtaactcgag atacgccata acaacggccc
    12241 caaagaaaaa ccacacagca atatttttac tccaaagcat ccgtaacagc atcgccatcc
    12301 caactgccgg cagcagattt ccagcgactg ttaatccgtt catgatgacc tctggaatat
    12361 tatccaagac gcttttgaca acatctgctc caaataaaac ggcaaagaat ggaataagag
    12421 cataaagcgc atagtggaca aaccacataa agaagtgcag attgatgatt cctttttgat
    12481 caccagattt tgcaaatttg aaaaatttag cagccatcgg tccacataca aaagaaaaaa
    12541 tcatattcat taactgtaat cctaatactg ctagtggaaa gacaatggga ataatcgcat
    12601 ccacgtttcc tcccattttg attgcgaacg ctgtagccag cccagcagcc aaagaaggct
    12661 ccgccgtact agagattccg atattgacga cacccataaa gatggcttct agtgacgccc
    12721 cgacaatcaa ccccgtctgc aaatccccca tcaacgctcc tgctaacgga gcaacaacaa
    12781 tcggacgact cagcatggac atcccgaaca tttcatgtcc agcaactcca ataaaaacgg
    12841 ccaaagcgac tacaaatgca tcatacataa tttttctcct tcctttttct aaaaatcact
    12901 caaaatagag atagctgaac gtttttccat cgccggtgta ggctgaaaaa cggtatctgg
    12961 ataaagttca actaattctt ttaagtcttg aatttctgac gtcgttaaca tcacttcctt
    13021 tgataaggtt ttcttatctt ccgatggcgg tgtcattttc ccagcattcc ccacgttcat
    13081 taattcgatt tccgttggga aagctttcgc aacttttacc gcatctttga cattagggac
    13141 taaaaccatc aagcgcatgt ccttcgcccg cggatctttt aacaaatgaa tcgcgtcatc
    13201 aactgtttta ataatgactt tgacattcga tggtgcagcc atcttcaatg tcatcttttg
    13261 aatatcgttg gtagccgcct catcgcttgc tactactaac ccggttaaat ttagactctt
    13321 tgtccaagtc atcgctactt gtccatgaat taatcgttca tcaattcgca ctgctttaat
    13381 catttttctt cctcctaaaa aaaatcttcc tcttcattta ctacatgtac ctcatcaaga
    13441 atggtcatct ttaattcctg ttggcattta tttaccagtt cctgaatatc ctcctttgtt
    13501 aattccttgg ttgccaatac tatttcgagc agaattggca gattaaagcc agacaaaata
    13561 gttacagcaa tctctttctc caaaaaggtt ttggtaatct tctgattgac acttccgcca
    13621 aataaatcgg taaaaacaaa aatctgatcc tgctcgttca cctggtttac aaatacattc
    13681 aactcctgtt caaaatcaca ctcatccaca taagcattaa tcaccgtaat ctcttgaacc
    13741 ttccccgtca gtaattgaat actgctaaga aagcctgaag ctaagtgacc gtgagtcgct
    13801 attagaattt tcttcatatg gttaattcct ttctttctgt tcacttttat ataagcaatt
    13861 cctgtgccaa cttttaaaaa cattggttta atcacgttaa ttcacattaa aaaaagtgtc
    13921 taacctaaaa aaaacagatt agacactttt ttagacactt tatttttgaa tatacggtcc
    13981 taataattca tagagcagcg aaagctcata agcattgact ttaaaattat atttcatctc
    14041 cattgattga aaaatacttc tagaaacata ataaaattca tcttcatctg gtttcaaatc
    14101 ataattcggt tcttcagatt tttctgtccg agccaagaat aaacgttcca tcatcaatgc
    14161 cgtatgcata tagagattta gttttacacg gccatttaaa gtgaaatgat agtaattttc
    14221 atatttagaa atgactgttt ccacttcact catcacgatc gttgggttca gaaagttcaa
    14281 acggtccgca attccttcca acgaaaagaa ttttactagc tcctgaatca agcggttaaa
    14341 ttctgccggc ttcatttcag gactcaaaat atgctccaaa ttggctgctc catcaccgtc
    14401 taagatgtca taaacattaa tattaggaat tgaaaaggaa cgtggaagat ccgaagtcgt
    14461 gatgaccaat tttgtcttgt caaaataagt ttcatcattt tggtcgatta attcacgtag
    14521 ttctctgtag tccttcgtga aaacttcgat ggattcatcc gataaatgat gacgaaaaac
    14581 atctttcaat ttgtccgaaa tccccaaacc cgacatacag gaaatgataa tgttgctact
    14641 ttgagaaaag ccttcaaagt attgaacatt gattgaatac ttgtctgctg catcctccgc
    14701 aatctgttta aatggcgcat tcatcgtcat cttcaagcca atatcaagtg caatggaagt
    14761 cgtcaaatta ttcagaagaa gtaactcccc ttttaaatcg ttcttgatct gtgtgtaaat
    14821 ttgatttaaa gagcccatat ctacgatcag cactagacta tctgtattca cttgccgttt
    14881 cacgaaattc cttgtcgatt cgacaatctc tgacacatta ctctctactg gcatatccaa
    14941 cgcttcaaaa acataattgc cgcataattg attcactact gcttgaatgc tgctagccgt
    15001 actatctccg tgagcaagca acaggccctt cagctcgatg gattcatcta cataatcaga
    15061 taaagtcacg acaataagca gtcttagaaa gttctcgtcc ttttctgaga gcggtggtaa
    15121 atgctccaag aactgattac ataagtaagt cgtccgaggt agatttgcag ctaatgtacg
    15181 atttatcccg gttaagtctg tcttatagct tggggaaatg acataggatc tgctaataat
    15241 ttctgcaaca ggtccactcg tcgtcaatcc gtacgttttt cctaaaacgg atacccagac
    15301 ctttctaaac atctgaatca tcaattttct actcattgat aaaaaagaat ccttatcaat
    15361 gtgatgcacc aaatgctgta cttcattctt tatcgaagaa aaattttcat tgaaattacc
    15421 ttcatttatc agctgagtaa ttttttccaa aatcgaatca taatcaaacg gtttgattcg
    15481 atccgttttt atcttttgat ttttctttat caataaacta gcaatcggca agctttctac
    15541 ttcggattcc agcatattta actgaatcaa gatctcattt ttcccaatcg ttaatggttc
    15601 ttcttgtgtt cgactataca catccgcaca actgatttta atgatattct tcagcttacc
    15661 aatattccca ctaaagggat gagataacat taaagacaag gcttctcttg aaacaataat
    15721 atctttttgt aaaacatccg cttcattttg aaaaaataat tgaatcagct ctaatcgttc
    15781 tcgaatgggg cgatcttcaa aagttggcaa cataacacta atttgaatac gccgatcaaa
    15841 ggtgtctaat aaataatttt cacctttttc cgtcgtggcg aaaataaaac gaaccttagc
    15901 agaatgccaa tttacatttt caccgactgg acgatagttt ccagtatcaa taaatagaaa
    15961 gagcttttct tgattttctt tcgataggcg atggacctca tcgagaaaaa gatagccgtt
    16021 gttggccgtt gccaataaac catcggtatc cttttcagcc cccgtaaagg cccccttctt
    16081 gtatccaaac agtgttgccg aaagcagttc gggattgtcc gcataatcgg cacagtttaa
    16141 gatagcaaat ggtgcatctt tttcaataat ttgctcatgg atagcgtatt caaaaatttt
    16201 tgtggctaaa aaactttttc caacacccgt ggctccattt attaatacac tgagtccgtt
    16261 aggcggatat ttgacagcgg ctatacattt ttgaatgacc tctctcagac ttccgtcatg
    16321 accaactaca gaatgaaagg cttcgtttat ttgctccgta ttatctacaa ccgcatattc
    16381 atctaaaggc ttccacaaaa caggctttcc cggtaatttc tcgacctttt tatcttccac
    16441 taatctcgtc aaataatggc tggcattctg acgagagata cctagattct tcgaaatttt
    16501 ctcggcagtg acagaagaca tattccctgt tagtcgtatc agctcatcaa aaactcgatc
    16561 tttagcgctc tttttcattt tttcctccag tttaatgtgt ctaaaagcat aataaactat
    16621 cggctgcaat atgtctattc tatatttgta ttccaatttt tactttgaaa cacaaatagt
    16681 cgaatatttg cctcagatat ctgacaaaat aatttatcta ccaatttagt ttttatctaa
    16741 tatgattttt atgttttttc atagctacaa atattgaact ggattcatat attagtttta
    16801 ttaatagatt acagaagggt taagtatatt tgcatagttt ccaattactt tatccttctg
    16861 taactttttc aatcttagat ttactagccc gtctcgggcc ctattttaca atggttttgt
    16921 ccacttttct ctggtagaaa aacgtggtag aaaaaataaa ataacacaaa aaaagcggcg
    16981 tttcatccgc ttttggtaga agattggaaa actcagatag aaaaaggacg atttttagct
    17041 ctttagaacg ttgataagtc cccattctta ctgagttttg ccattatctc ctatacatct
    17101 tgaattctaa gaataggtca ttatagatgc ctaggtattt cgatcctaga tctttgtaga
    17161 cttcaagatg ggagatcgtg tcatcagatg gatagaattg ttcgtccgac gaaatgtctt
    17221 ttggcaataa cgcttttgct tcttcatttg gtgtagaata accaatatac tctgcatttt
    17281 gtgccgcatt ttccggttct agcataaagt tgatgaaatc ataggcacct tctttatttt
    17341 tcacggtttt tggcataacg atattatcaa accaaagatt ggatccttct ggtgggatca
    17401 cataatgtaa atgttcattt tcccacatca tatcggcagc ttcgccagaa aatgtcactg
    17461 ccacggaggc ttcttcattg atcatataca ttttgatctc atctgccacg attgctttga
    17521 catttggtgt taaatgattc aatttatttg ctgcttctgt cagctgtgcc atattcttac
    17581 tattcaaaga atagcctaaa ctattcaaag aaagccccat aacttctcgt gcaccgtcaa
    17641 tcagcatcaa actgtctttt aattccggct tccacaaatc atcccaatgc ttaatttgat
    17701 ttgcatcaaa gaatttatcg ttatatacga tccctaaagt tccccaaaaa tatggaatcg
    17761 aatactgatt atctggatca aaatccaagt ctaaaaatcg tggatcaata ttatttaatc
    17821 ctttgatctt cgaatgatct aatggaagta ctagtttttc tttgatcatt ttttggatca
    17881 tatattcact tggtatagcg atgtcgtagt ttgtcccacc ttgctggatt tttgtgaaca
    17941 tcgcttcatt agaatcaaat gtttcataat tcactttgta tccatattct ttttcaaatt
    18001 tcgtgatcaa actaggatcg atataatctc cccagttata gatggtaagt acctttgctc
    18061 cactcatgcc acttgttttt tcaagttgac gaacaccaag cagcagcacc gcaataatga
    18121 tgagaatacc taaaaacaga gattgtaatt ttttcatcgc agccgtgcca ccccctcgcg
    18181 tactcttctg tttttcttca gccgttttgg acggttttct ttactaataa agtagtatcc
    18241 aatgaccaat atcatggaga acaagaaaac taacgcactt agcgcattga tttctaaact
    18301 gattccttgt cttgcacgag aatagatctc tactgagagc gtcgtaaagc catttcctgt
    18361 gacgaaaaat gtaaccgcaa aatcatcaag agaataggta aatgccataa aatatccagc
    18421 tatgattcct ggagaaagat aaggcaaaat gatatttttg actacttgga aattattagc
    18481 accaaggtct ctagccgcat cgaccattga atcgttcatt tcctgcaatt ttggcaagac
    18541 catcaagacg acgattggaa tgctgaaagc aatatgggaa agcaatacac tctgaaaacc
    18601 taatccgaat cctagtagtg taaagaaaat caagaaacta gcaccgataa taacatcagg
    18661 agagactaat aagatgttat tcatgctcaa caacgctgtc cgtgcacgtc gttttttcgt
    18721 gtaatagatt cccatagccc caaatgtccc gatgatcgtg gcgatcaatg cagataagaa
    18781 agccagcatg aacgtattca atacgataat gatcaaacgt gtatcttcaa aaacagcttc
    18841 ataattttcc caagtaaatc ctgtaaagct gttcatcgtt cctccttcat tgaaggaata
    18901 aaaaatcaga tagaagatcg gtgcatataa gaataaaaag acgatcccta aataaagata
    18961 cgaccattta aattttttca tttatgagcg acctcctttt ttcttctcgc ctgtcaggag
    19021 catcacgatg aacatagcaa tgattaaaat cacaccgatt gtagaaccca tcccccagtt
    19081 ttgtgtaacc aagaaatgct gttcgatagc cgttcctaaa gtgatcaccc gatttccgcc
    19141 aatcagacga gtcagcatga ataaagaaag agaaggaata aatactgctt ggacaccact
    19201 tttcacacca tttaatgata acggaaaaat cactcgtcgg aaggtctcaa agctattcgc
    19261 gcctaaatct ctactagcac tgatcaaaga aggattcatc tcttccaatg cattgaaaat
    19321 tggcataatc ataaatggaa tctcgatata cgttgccacg aacagaaaac tgaagtctgt
    19381 gaataatatc tgtttcggcc cgatcccgat aaaatcaaag aaatgattca cgctgccgtg
    19441 cacactgaaa atcccaataa atgcgtaagc tttaagcagc agattcaccc atgtaggcaa
    19501 aataatcagc atcagccata attgcttgtg cttcagtttt gttaaaaaat aagcagtcgg
    19561 gtaactgatg atcagcgtga aaaacgtgat caaaaatgca taccatacag aattgatcgt
    19621 catagaaagc gatgtccccg acgtaaaata agatagataa ttattcagcg tgaattgccc
    19681 attcatatca aaaaaagatt gataaatgat cattaataca ggcgcgatca cgaataaacc
    19741 gagccacaaa atataaggga cggaatagat cctgcgcatt gttttcattc ttgtcccccc
    19801 tattcttcgt agctgtcaag acgtgcatcg aattcttctt cagactcgtt gaaacgcatg
    19861 acatggatat cttctggctc aaaggccaat cctactttgc ttccttcttt agcttttttc
    19921 gttgaatgga ccatccattc attttgctgt tcatcataac agatgatttc ataatggaca
    19981 ccacgaaaca gctgagtatc gaccgttacg actagttttc ctttatctgg tgtcgtgatc
    20041 gtcaaatcct ctgggcgtaa aacaacttca actggttcat ttagacgcat cccgccatcg
    20101 acacattcaa attgcttacc aacaaactca actagattat cttcgatcat tacaccgtcc
    20161 acgatattgc tctccccaac aaaatcagca acgaaatgat tgattggctc atcataaata
    20221 tctacgggtg taccactttg gacgattttc cctttattca tgacaaaaat ctcatcactc
    20281 attgccaacg cttcttcttg gtcatgagta acaaaaataa acgtgattcc taatcgttgt
    20341 tgcagatccc gcagttcata ctgcatatct gtacgtagtt tcaaatcaag cgccgataac
    20401 ggttcatcta atagcaatac tttcggttca tttacaattg ctcgtgcaat cgctacacgc
    20461 tgtctttgac caccagacat ttcactaatc tcccgttttt cgtatccagg aagctggacc
    20521 atacgcaaag catcttttac cttcttttcg atatctgatt tggacatttt cttgatcttc
    20581 aatccaaaag caacgttatc aaaaacattc atatgaggaa aaagtgcata atcttggaag
    20641 actgtattta cttgtcgctt attagctggc aaatcattca accgtttccc ttcaaaatag
    20701 atgtcccctt ctgtagcttc ggtgaatcca gctataatac gtaggatcgt tgttttacca
    20761 cagccggaag gaccaagcag tgtataaaat tttccttgtt cgatctcaaa acttactttt
    20821 tttaatactg tttcatcatc atagcgtttc actacttgat caaatgaaat gattggtttc
    20881 cccacgtttt taccccctat attacaaata tgattgcgtt gcaacaatca atacttttgt
    20941 tcgttttttg agatgattcc ttaattgatg ttgttttgtc gcttgataat agatcgactg
    21001 tcctgctttt gccacataga ccgtctctcc taagcttaac tctaattccc cttctaatac
    21061 aaaaataaac gtttcagata atgaaggttc aaacgtctta tattctcctg caccttcaaa
    21121 agtcagcaag acaggctcca tttctttttc attcgaatca gccacgagcc attccaattc
    21181 atacccgttt tcttcatcga aataaaccgt atgatcttct tttgaataaa ccacttgcag
    21241 attcgctgct tcttgatgaa aaaattcttc tggagtcaca cctaagactt caagaataga
    21301 aaagaatgtt tccattgatg gtgaacttaa atctcgttcc aactgggaaa tatacccttt
    21361 tgacaaatct gtgcgttcgc ctagttcttc ttgtgtcaaa ttcttttgga tgcgcaaatt
    21421 tcttaacttc tcaccaattt ccataatttc ctccgaatcg aagttttcta atagtaaact
    21481 tcctgtttac taccactcgt attagtatac gaaatttatt gaaaaaggca accctttgta
    21541 aaattttttt tgctgaatat ttctttatct tttggaataa attctagttg tttgattctt
    21601 tatttttcac ctaatttttg tctaaattta cggaagaaca agaataaaag agtaggcttt
    21661 atggtatgat aaaagaaaat tttaaaagga gtgtctcacg aatgaacgct tttcgatctt
    21721 ttcaggtaat aaaggaacct gttttccata cagcaatcag tgatctgcca tttactgagt
    21781 tggctgaaaa tgaagtcttg gtaaaaattg cctattctga tgtcaattat aaagatgcat
    21841 tggcaatgag cgagtctggt caggtgatac gaacatatcc gatgactcca gggattgatt
    21901 tatccggaac tgtcgtccaa agtaacaatc cacgtttttc aaaagaagat cttgtacttg
    21961 ctactggttt tgggcttggc gtcacccatc caggcggtta tagccaatat caaaaagtac
    22021 caggagactg gttagttcct ctaccaaaaa atatgacgct gcgtcaagcg atgatccttg
    22081 gtacagcagg gtttactgct atgttgtgtg tgaacgcttt aatccgtcaa ggtatgacct
    22141 ccgataaaaa agttgtcgtt actggtgctt ctggcggtgt aggcagtaca gcgatagccc
    22201 tgttacataa actaggattc cagtcaatca ttgccttttc cagaaaagaa gagtctgtca
    22261 catggctaaa aagtctaggt gctttgcaag tcgtacgacc tgatgaattt ctgccagaga
    22321 caacaaaagc tttaggaaaa cagcaaatcg actatgtcat cgacacagta ggtggcgagc
    22381 agctaagcag tcttcttcca ttgatctctt ataacggtgc agtagcttta tgtggcaatg
    22441 ctgggggaat caaactaaac gcgactgtct tgccttttat tctgcgaaac atccagttga
    22501 ttggaattga ttctgtcaat gtaccaatag atcaacgtct ttctttatgg caacagatgg
    22561 cggatttgca gattgcagat gaactggtcg tccaagagat tactctggat caattgcctg
    22621 aaacagcatc taaactactt gctggaacac accaaggaag aacactagta aatgtaggtg
    22681 accataaatg aagattctaa tcacagctgg aggaacgact gaaaaaatcg atcaagtacg
    22741 tgctattaca aatcattcga caggacgatt agggcaagca ttagctgatt atttagctgc
    22801 caatccggat accacaatcg attacgtgac tacgaaacaa gcactgaaac cgaaaagaca
    22861 ctccaatata acgatttata cgatcgaatc tgcactggac ttatttttac aattagaaga
    22921 actaacgaaa aaagaacact acgatgcgat tatccacagt atggcagtca gtgattttac
    22981 tcctgctttt agtttttcag aagaacagct ggcaaaaaac ctacccactt cttcaacgca
    23041 agaagaactg gacaattggt ttgcggaaaa tgaacagaca gataccactg tttcaaagat
    23101 ctcttctaac accgagcatc ttgtgctcgt tttgaagaaa acaccaaaaa tcatttctta
    23161 tctacgtgaa tggcagccaa aagcaaaaat cattggtttc aaactattag tagatgtacc
    23221 aaaggaatct cttctggcag tcgcaaaaaa tagtctgata aataacaaaa ctgattttat
    23281 tttcgcaaat gatttgacgg aaatccatgg cgagacacac cacggctatc tcttatctaa
    23341 agatggaaca gtggaagaag ctcaaagtaa atctgagatt gctgcattga ttactgagaa
    23401 aattagactg gaggaatcaa aatgaaaaag atcttactag gcgttactgg aagtatttct
    23461 gcttacaaaa gcgctgatct tacaagtcaa ttcgtcaaat taggtgccca ggtagatgtg
    23521 attatgtcta aaagcagcac acattttatc acgccattaa ccttgcaatc tctgtcgaag
    23581 cgcgcagtcc atacagacgt catgcaagaa tcaaatccgc aggtaatcaa tcatatcgaa
    23641 ctagcaaaac aagctgactt gtttctcatc gcgcctgcta cagcaaacat tatcggcaag
    23701 ctagcaaacg gcattgcaga cgatcttctt tcaacagtcg caatggcgct tccagtggga
    23761 acaccaaaat tgattgcccc tgccatgaac acgaatatgt atcaaaaccc gatcactcag
    23821 cgtaatctgg aaacattgag atctgtcggc tatcaggaaa ttgaaccaag agaatctctt
    23881 ttagcttgtg gagattttgg aaaaggtgcc ttagctgata ttcagacgat catcgaacag
    23941 gtcatgctga ctcttaatga aagaaatgct tagaaaaaag gagtacttat gaaaaataca
    24001 aaaaacttta cattaactgc gatgttttta gctattttga ttttattagc tgttacgcct
    24061 ttaggtttta tccctatcgg tccaatcaat gcaacaacca tgcatattcc tgtaatcatt
    24121 gcttcgatcg ttttaggacc aagactaggt gcttttcttg gcggtacatt tggactgatc
    24181 agcatgatcc gtagcacagt tatccaaaca cctttgtctt ttgtcttttc tccgtttatc
    24241 ccagtcatcg gaacagaaca tggaagctgg aaagccttgc tgatcgcgtt tattcctcgc
    24301 atcttgattg gggtggttcc ttatttcgtt tataaaggtc tgcgcaaact aatgaaacaa
    24361 aaagcagatt ccgtttcttt attcttagct ggactttccg gttcattgac caatacgatt
    24421 ttagtgatga atatgatcta ctttttattc cagcaagatt atgccaaagt catcggaaca
    24481 aatttaaatg ctgtttattc agctattcta gcggttattt ttacaagtgg tatacctgaa
    24541 ggaatcgtcg caggtcttgc gactgcagca gtatgcaatg tcttgttaag atacttcaag
    24601 cacccttctc ttactaactg atagtcatcg tagccgatct ctgatttttt tctcttttgg
    24661 tgttactatt gaatggaaag atttgacgtc gaaaggatga tccaaatgaa aatttttgtt
    24721 gtaggtgcaa acggacaaat tggccgacac ctgataaaag atttagctcc aagttcccat
    24781 gaaatttttg caggggtgag agatgttgct tcccaaacat tagtaaaaaa agagaatgtt
    24841 tcgtatgtct cttttgattt aacttggtcc gtggaaaaaa tgaccgaagc attcaaaggc
    24901 atcgatgtat tgatttttgc tgccggctct caaggaaaga atcttctgca agtagacttg
    24961 gatggcgcaa tcaaaacagt gatcgcagct gaaaatgcgc atgtctctcg ctatctgatg
    25021 gtcagtgctg tttatgcaga tgaaccagca aaatggcctg agtccatgac agattattat
    25081 attactaaac attatgcaga tgaatggctg aaacgaacta acttagattt tgtcattctt
    25141 caaccagtca cattgactaa cgatgaagaa gttacttcca ttcaattaac taagcctaat
    25201 gagaaggcat caaaaacgat cactcgctct acagttgctg ctgtacttgc cgctctagtg
    25261 gaagaaacgg atatttctcg tacgactcta gtactttctg aaggtagcaa agagttgaat
    25321 acagcattcc aagaatgggc aaaggaggaa tagatccatg cgtgccattt caatcaagca
    25381 tcctggttct gtagatgtat tagaacaaat tgaagtgcct gttcctaaac caaaacaagg
    25441 cgaattactg atcaaagtcc atacagcagc tgtcaatcga acagatatta tgaaaagaga
    25501 atcagtctct ttgaaacctc cctaccctat tttgggagtc gaagtagctg gagaggtagt
    25561 tgagaataaa agtgatatct ccacttttac tcctgggaca aaagtatacg gattagtcaa
    25621 tctaggtggt tatgccgaat atgctgtgat gcctgccgat cgtgcaatcc ttctccctga
    25681 tacgctagac tatgtttctg ctgctggaat ctcagaagtt tttttgactg cttatcaaac
    25741 actttactgg cttggaaaat tacaagaagg cgaaactgtc ttgatccatg caggagcaag
    25801 tggtgtggga acagcagcga tccaattagc tagacagctt tctcacgcga aaatcatcgt
    25861 tactgcagga tcagaagaaa aactcgcctt ttgtaaagaa cttggtgcag atgaattgat
    25921 caactataaa aaacaagact ttcaagaaga ggtacgaaaa atcactgaag gtcggggagt
    25981 cgatgttatt ttggatttca tcggcgcttc ttactgggaa aagaatttag cttctatagc
    26041 cgttgatgga cgttgggtct tgattggtat gctaggtggg accatcgtgc ctgagataga
    26101 ccttggtgta ttgattggga aacgtgtcca actgcttggt acattattga cacctagaag
    26161 tgatcagtat aaagcagagc tcgcacaaac ttttatgcaa aaagctgggc cttttctaga
    26221 aaatggcaag attcggccaa tcattgaccg tacattccaa ttcagcgcag tcaaacaagc
    26281 gcatgaatat atggaagcaa acaaaaacat cggaaaaatc attttgaaga taactgaata
    26341 aaatcatgct gatcaaaaaa gaggctggga cagaagcgtt taactccaag aaataagaag
    26401 aaattcacga aaattgcttt tcaaattttt gtgaatttca gcttatttcc gaaggaattt
    26461 cttctgcttc cgccgtttat acgtgtttag agcgtgagac aaaagtgttc tttacttttg
    26521 tcccacgctc tttttccgaa taaacggtgt tca
//
LOCUS       DACDYI010000043        26466 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.043, whole genome shotgun sequence.
ACCESSION   DACDYI010000043 DACDYI010000000
VERSION     DACDYI010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.043"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(324..893)
                     /locus_tag="IWM52_001679"
     CDS             complement(324..893)
                     /locus_tag="IWM52_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9973930.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(967..2256)
                     /locus_tag="IWM52_001680"
     CDS             complement(967..2256)
                     /locus_tag="IWM52_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973931.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESENKSKLTKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQAFAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            2610..3458
                     /locus_tag="IWM52_001681"
     CDS             2610..3458
                     /locus_tag="IWM52_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAP9973932.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPVPVR"
     gene            3472..4545
                     /locus_tag="IWM52_001682"
     CDS             3472..4545
                     /locus_tag="IWM52_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAP9973933.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKREISFPVTVL
                     PAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKLSAHTQEWLY
                     REYDYQEEECSPLFCFWGSHSPKFEPLLDLYFSLWHLRKEKQPELLILGNRAFHGKDP
                     GHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKDRTVYLVYFV
                     EKQLMVQEKDKICSLPGGEWIDD"
     gene            4535..6706
                     /locus_tag="IWM52_001683"
     CDS             4535..6706
                     /locus_tag="IWM52_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HAP9973934.1"
                     /translation="MTIKWEKWLCGVYSFVILSVGAPIFVVANQLQNSYLLILTIGVL
                     CLIVCCIPKKWVRLLLSLPVMIGCLFLYFPSKTFSLLWILQFFSSVTEEFFHLSQGKL
                     YYLPEKTAFFLIMLLIYLFITLTVDYDYLYAPFLSLMAYFMMLTVFREKDLLYEMIAV
                     VTVLFVYLAARQFFSMRLLSGNTRFQSLTTVLLICFLTISAILPLYLPKVHQSLEETS
                     EPIREYLNDEGLYDTLHEYQLTGSARTGFSENDEQLGGPLYDNGEVVFTANQKGNGYW
                     KIENKNHYTGSGWIEDQDAPIQPIQEFPVDITSYQTVMEDKENITLTLKKQAFLPYPY
                     GNTTLTDFSLASGQTLYYLPESQRFIPVPNESTRSATLQTERLVFTREELKSSQLKAD
                     DLENQQTNLQLPKSLPKRIEDLAVELTKNEATLIRKVEAVEQYLKTSGNFRYSKTDTG
                     HTPKEHDYVDYFLFDSKVGYCDNFSTSMVVLLRTLGIPARWTKGFTNGLRTGTDNQGN
                     EEYSILNSHAHSWPEVYFPGFGWLPFEPTPSFANPMGSQPVAETTETVQPAIEQPNES
                     ENMAETISSERSEVIPSEETTLTPAYKIVLFSLGVCLYVLLFFLLLFRKSLRWTSYLI
                     YLHFFASTNTIAYQKLLNHLETLVPRKPAESLQEYSLRIEQEFKLDLRFIRLTTQYEA
                     EIYGRQADPVNFDKKTYLEITHIIRKTVYKKVLTKGSRFKK"
     gene            complement(6818..8989)
                     /gene="copB"
                     /locus_tag="IWM52_001684"
     CDS             complement(6818..8989)
                     /gene="copB"
                     /locus_tag="IWM52_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAP9973935.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHDHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKNQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9009..11195)
                     /locus_tag="IWM52_001685"
     CDS             complement(9009..11195)
                     /locus_tag="IWM52_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAP9973936.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKSKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMSSSLASIAQTIELSRVTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(11195..11404)
                     /gene="copZ"
                     /locus_tag="IWM52_001686"
     CDS             complement(11195..11404)
                     /gene="copZ"
                     /locus_tag="IWM52_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAP9973937.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(11417..11857)
                     /locus_tag="IWM52_001687"
     CDS             complement(11417..11857)
                     /locus_tag="IWM52_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAP9973938.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(11931..12404)
                     /locus_tag="IWM52_001688"
     CDS             complement(11931..12404)
                     /locus_tag="IWM52_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
                     /protein_id="HAP9973939.1"
                     /translation="MGRENRPSQGRSKKSPAFRIQAKKWFLQEQQSFMVSNPSWIVEG
                     NYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLKRKINKKSRPDMPESFSEKLNRK
                     YLEFLSFIWHFEGNNEPKIQQLIQVANARNKLIILKTRREKQEFLTKLKIESKNN"
     gene            complement(12714..14180)
                     /locus_tag="IWM52_001689"
     CDS             complement(12714..14180)
                     /locus_tag="IWM52_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9973940.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(14491..16575)
                     /gene="cadA"
                     /locus_tag="IWM52_001690"
     CDS             complement(14491..16575)
                     /gene="cadA"
                     /locus_tag="IWM52_001690"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP9973941.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKKRNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     RAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDPTGIDPKKFMQYVASAEQFSTHPIAQSVLEGYAGPLL
                     PTANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGY
                     LVIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDK
                     VQRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQP
                     SKIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVL
                     NAMRSLHVKNKR"
     gene            complement(16717..16905)
                     /locus_tag="IWM52_001691"
                     /pseudo
     CDS             complement(16717..16905)
                     /locus_tag="IWM52_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329861.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            complement(17099..17458)
                     /locus_tag="IWM52_001692"
     CDS             complement(17099..17458)
                     /locus_tag="IWM52_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAP9973942.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(17488..17829)
                     /locus_tag="IWM52_001693"
     CDS             complement(17488..17829)
                     /locus_tag="IWM52_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973943.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(17826..18500)
                     /locus_tag="IWM52_001694"
     CDS             complement(17826..18500)
                     /locus_tag="IWM52_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP9973944.1"
                     /translation="MKKRILFIGDSITDAGRYRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIVAVDFFSGDKNE
                     RKKSEK"
     gene            complement(19296..20594)
                     /gene="asnS"
                     /locus_tag="IWM52_001695"
     CDS             complement(19296..20594)
                     /gene="asnS"
                     /locus_tag="IWM52_001695"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAP9973945.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKITFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(20634..21824)
                     /locus_tag="IWM52_001696"
     CDS             complement(20634..21824)
                     /locus_tag="IWM52_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9973946.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(21845..22372)
                     /locus_tag="IWM52_001697"
     CDS             complement(21845..22372)
                     /locus_tag="IWM52_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP9973947.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(22419..25208)
                     /locus_tag="IWM52_001698"
     CDS             complement(22419..25208)
                     /locus_tag="IWM52_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP9973948.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(25355..25555)
                     /locus_tag="IWM52_001699"
     CDS             complement(25355..25555)
                     /locus_tag="IWM52_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9973949.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(26007..26327)
                     /locus_tag="IWM52_001700"
     CDS             complement(26007..26327)
                     /locus_tag="IWM52_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973950.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
BASE COUNT         7455 a         5604 c         4647 g         8760 t
ORIGIN      
        1 gtcaaggaca atcggaatag catacggaaa gaagttctgc accttataaa atttgtcaaa
       61 aaaactgttg ccattccaag tgaaaaaaag atcaatgaat caactagaaa actatataga
      121 agagcaaata aaaaagcact caaattgagt gcttttttaa gctcactatc actaaaacta
      181 tcactaattt tatttataag cgaacatgtt tctatcactg attttttaaa tctagcgaaa
      241 aacccttatc ttaaaggcgt tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc
      301 ctagaagtgc gaacatggtt ttcttatagc tcatgtagaa aaattttctt gatgatgatt
      361 ttatccatca attgagattg actgttttgc tctcctatga tttcagctat gattgtccga
      421 ttttgcggtt ttcttcttaa ctggtagttg attttcccag tttccatcaa ctcgctaagc
      481 atatccacga ctccgccaaa tccggtcttt ttcgtaataa aacctagtgc taccattcgt
      541 tcatctagat tttttgctgt aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg
      601 gccagctctt ccatcccttc agcattggat acgacggaag ctgttttgtt aataggttct
      661 tcgtttattt cttttggttc tttcctctcc actttcactg tttgacgaag gacatctagt
      721 tcttgcaaga gcgtttcgta ttgttcttgg gtcagcatca cgccagctac tttttcacga
      781 ttaaatatat atacgccagt tcccgcgtct cgcgcttgag caaatacatc catcggcgat
      841 cgtttcactt ctgtgatcga agatgtggga acttctaatt tctttaattc caatggattt
      901 cctcctttac taatcgcttt ttattatagc acagactgac ttgttttgat cttttttcca
      961 aaggttttat cggtttttgc ctaaccagta attgatatcg gtaagaagtc tttcgatacc
     1021 aaatgaagga tggtacagat acgttttttg atcttcgtta tggtctagtg gaaaaaaatg
     1081 atcggtgatc actagatctg cataccgagg cggcttattt cgagtacata catgtataga
     1141 gatccgtttt cgatgtctta gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg
     1201 gtgttcatag cgaaaaaaaa agacgatttt cacttcagga gggtggacat ctaaataccc
     1261 tcgttcctgg agtgttttca gtaaaagcat cccccactct actgcggggt gtgcaaatgc
     1321 ttgttggatc gctagccgaa attgatatag tttatctttg gaattgggaa atttttctaa
     1381 tgtttcctga taataacgaa cttcttctat cagccggtca tggataccaa ttacgtaacg
     1441 aagccattct tggtaaagta cttttgtcag ctttgatttg ttttcgctct caaattcgct
     1501 attcagccaa aacagcgaat caagcttttc agtcacaatc gtcaatttcc gatagtcttg
     1561 tgtctcttct atccgtttcc accgcttttc agtaatctgg ttacagctca agcgcaaaat
     1621 tgctagataa tttttttctt ttattggcaa aatctgctct tctctaaata gcaattcagg
     1681 aaacaattca aagaatagtg ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt
     1741 tctttgcttc aataattgga tgaacatctc agaaaaggac tgctctccat catctgcaaa
     1801 aaaatgcgga aaatctttca aaaagcaaaa cagcagatat cttacttttc tttctgaacc
     1861 aaagatctgt gcttttttat aagaaaaaga aaggtggtac tcgccaaacc actttctcaa
     1921 tcgctcttgc gtagaacgat cgtacttctg ctcgtaaggt tcattcaata gcagattttt
     1981 cagtaaacaa aacgggggtg attggatcag aaaaacaaac aagagataag gttcgctata
     2041 tccttgatca taaactaact gaacggtttt cttatgaaga atcagctgga tgggacagtc
     2101 gctttccgcc aaacgtttca gatgccgatc cactgtttta acatcgattt tcagttcttc
     2161 agctaaatca gcttttcgcc agatcaaatc tttcattaga ttgattagtg aaaataaatt
     2221 ctgtgcattt tttgataata gtttttgcca tctcatcgat catttctcac ctgatttcta
     2281 aacatattgt ttgatcaaaa tgggttcact cctaaaaaga taaaaaactc cctttctatt
     2341 tatttaaata cgcaaatttt cctcattatt tttaatttta ctcaatttaa tcgtattctc
     2401 atttcaaaac gaatgtactt taaaaaaaca taataaaatc actttgcttg taggatgtat
     2461 tcccttccat tacaatgaaa taaatagcaa ttttatgaga aggtgatgtg aaaatggaag
     2521 aaaaaatcga agaatccaaa caaaaaatcg atcagttgat acaagaatta gaaaaagcgg
     2581 tcatcggcaa acgttcaact atccaattga tgatcgtggc cctattagca ggtggtcatg
     2641 tcttattcga agatattccc ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag
     2701 ctgtacaggg aaccttcagc agagtccaat gtactcctga tctgctgcct agtgatattc
     2761 ttggtttttc cgtatacaat tcacaatcaa aagagtttga atttcgtccg ggaccgatct
     2821 ttaccacgat cttgttggca gatgagatca atcgcacgac tcctcgtact cagtcagcac
     2881 ttttagaagc aatggccgag aatcatgcaa cgatcgacaa caatacatac cctctagaca
     2941 atcatttttt tgtcttagct acgcaaaatc caatcgagta tgaaggaaca tatcctttgc
     3001 cggaagcaca gctcgatcgt tttttattcc gactacaaat cggttatcct tcttttgatg
     3061 acgaactttt gctactttta gataaattcg aaaaagtgtt ggataactta aatgaagtat
     3121 tgaacagtta tgaaatagaa gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg
     3181 aagtggcatc ttatgccttg cagttagtgt cagcaacaag aagccatgct gccatccagc
     3241 ttggcatcag tccacgagga agcctttcct ttattcaggc agcaaaagct tatgctttaa
     3301 tccatgggag aaattacgtc acgcccaaag atttgcagga tttagttcct tacgtgtttt
     3361 cccatcgcct gattttttac gatcgttcat tgaaggaaga cgaaaaaaaa caaatcattc
     3421 atactcttgt tgcacaaatt cctgttcctg tgaggtagat tgaggtagat aatggaaaaa
     3481 atcaaagatt atttcattaa ttttgtgtta tttctggtat atttcatcat tgtgcttttt
     3541 gctcttgtat tcaacaattc tttaggctgg ttcttgttgg ccttttttac tctttatgta
     3601 tcatggagct tgatttcatt aagcagcttt ctaaagaaac tttcggttac ttgtacattg
     3661 cagccactcg tttatcgcaa aaaaagtatg caggttattt tttccatagc aaaaaagaga
     3721 actttttctt tacctctccc tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa
     3781 aaacaagaga ttttgattct tacaaataaa ccgcaagaag tccaattgct ttggcagcca
     3841 aaagaaagag gatgccatga aacattaact gtcattttta cagcctatga tagtctagga
     3901 cttttcagaa aaagtgcaaa aagagagatc tcttttcctg tgactgttct gcctgctttt
     3961 tgtaagattc aagcagaaaa attacagaga gttctggaaa aaaaacagca gctgaacgcg
     4021 taccaaagag gtcttgattt tcgggaacat cgcgcttatc gtccaggtga ttctttcaac
     4081 cgaatcgatt ggaaattgtc tgctcatact caagagtggt tataccgtga atacgattat
     4141 caagaagaag aatgttctcc tcttttttgc ttttggggtt cgcacagtcc aaaatttgaa
     4201 cctctgctag acttgtattt ttctttatgg catttgagaa aagaaaagca gccggaactt
     4261 ctgatattag gcaaccgcgc ctttcatggc aaagaccccg gtcataccta ttttgcttct
     4321 ttggaagctg gcgatgagaa agcttttttc gcacacctaa aaaaatatgc aaaaaaacaa
     4381 atcgtcctct ttgttcctga gaattctcct gaagtctcag aagctattga aacattgtca
     4441 aaagatcgaa cggtttatct tgtttatttc gtagaaaaac aactcatggt ccaagaaaaa
     4501 gacaagatat gttcattgcc aggaggtgaa tggattgacg attaaatggg aaaaatggct
     4561 gtgtggcgtt tactcctttg tgatactaag tgtaggtgct ccgatatttg tagtagctaa
     4621 tcaattgcaa aacagctacc ttcttatcct aactattggt gtcctatgcc ttattgtctg
     4681 ctgtattcct aaaaaatggg tacgtttgct gctttctttg ccagtaatga taggttgttt
     4741 gtttttgtat tttccttcga agacattttc cttattatgg atcttacagt ttttttcgtc
     4801 agtcacagag gaattcttcc atctatccca aggtaaactt tattatttgc cagaaaagac
     4861 agcttttttt ctcataatgt tgctgatcta tttatttatt acattgaccg tggattacga
     4921 ctatttgtac gcaccctttc tttctttgat ggcttacttc atgatgctta ccgtttttcg
     4981 ggaaaaagat ttactgtacg aaatgattgc ggtagtaact gttttgttcg tgtatctggc
     5041 agcaaggcaa tttttctcca tgcggttgtt gtcgggaaac actcgattcc agtcattgac
     5101 tacagttctg cttatttgtt tcttaactat tagcgctatc ttgcctttat atcttccgaa
     5161 agtacaccaa tctctcgagg aaacgagtga accgatccga gaatatctta atgatgaagg
     5221 gctttatgat acactccatg agtatcaatt aaccggaagt gcaagaacag gcttcagtga
     5281 gaatgatgaa cagcttggtg gacctttata tgataatggc gaagttgttt ttacagcgaa
     5341 ccaaaaagga aatggctatt ggaagatcga aaataaaaat cattacaccg gttctggatg
     5401 gatagaagat caggacgcac ccatccaacc aatccaagaa ttccctgttg acatcacgag
     5461 ctaccaaacc gttatggaag acaaagaaaa cattacattg accctaaaaa aacaagcttt
     5521 cttaccttat ccttatggaa atactacctt gactgatttt tcattagcta gtggacaaac
     5581 gctttactat ttgcctgaaa gccagcgatt cattcctgtc ccgaatgaat cgacacgttc
     5641 tgcaacttta caaacagaaa ggttagtttt tacgagagaa gaattaaaaa gcagtcagtt
     5701 aaaagctgat gatttagaaa atcagcagac aaatcttcaa ttgccaaaaa gtttgcctaa
     5761 acgtattgag gacttggcgg tcgaattgac caaaaatgaa gcaactttaa ttcgtaaagt
     5821 cgaagctgtc gaacagtatt taaaaacaag cggaaatttc cgctattcca aaacggatac
     5881 cggacatact ccgaaagaac acgattatgt ggattatttc ctctttgatt caaaagtcgg
     5941 ctattgtgac aacttttcaa cttctatggt cgtccttctt cgaacacttg gtatcccagc
     6001 tcgctggaca aaaggtttta caaatgggct cagaacaggc acagataatc aagggaatga
     6061 ggaatacagc attctcaata gtcatgctca ttcttggcca gaggtatact ttcctggatt
     6121 tggctggctt ccatttgaac caaccccttc atttgccaat ccaatgggtt cacaaccagt
     6181 tgctgaaaca accgaaaccg tccagcctgc tatcgaacaa ccaaatgaaa gcgaaaatat
     6241 ggcagaaact atttccagtg agagatctga ggtcattcca tcagaagaaa caacactcac
     6301 accagcttat aaaatcgtct tattcagcct tggggtgtgt ttatacgtct tgttgttctt
     6361 tctattgtta ttcagaaaat ctctgcgctg gacaagttat cttatctatc ttcatttttt
     6421 tgcttctact aatacgattg catatcaaaa acttcttaac catttggaaa cattggtacc
     6481 gcgcaaaccc gcagaatctt tgcaggaata tagcctgcga atagaacaag aattcaagct
     6541 agatctgcgt ttcattcgat taactactca atatgaagca gaaatatatg gacgacaagc
     6601 agatccggta aatttcgata aaaaaaccta tttagaaatt acgcatataa taagaaaaac
     6661 ggtatacaaa aaagttctga caaaagggag ccgcttcaaa aaataagata aaatttctgg
     6721 agaatctact tctggaatgc cgagaaccag ctgcttgagt atcgattatt cgaaaaaaag
     6781 gttgtgacac gttttttgtc acaacctttt ttcattatta tttgattttc aatgtcaaag
     6841 catttagagc tacaactacc gtacttaacg acatcaagac agcaccaact gctggactta
     6901 atatgatacc aattggcgca agaattccgg ctgctagcgg aattgcaaga atattgtaac
     6961 ctgcccccca ccagaggttc tggatcattt tctttcgcgt ttgcttggct agatcaagaa
     7021 aatgcaaaat atcttttgga tcgctgtttg tcaatacgac atccgcagaa tcgattgcga
     7081 tatctgtccc tgcaccgatt gccatcccga ttgaagcacg ggctaaactt ggcgcatcat
     7141 tgatcccatc acccaccatg attacttttt tcccttggtc aaggtatttc tggacaacag
     7201 cttctttatc atctggtaat aaaccgccat aatattcttc gatccctaaa taatctgcta
     7261 ctgcttgggc agcgttttta ttgtcaccag taagcatgac aggagtgatt tttctttctt
     7321 tcaatgttcg gataaattct tttgcttctg tttttactac gtcacctaaa gcaacgattg
     7381 cagctagatg attttctaaa atcaggaagc tgatggtatt tccttgttct tggtaaggtt
     7441 tcaataatgc ttgttctacg tttaatccga gacgctcaac ttctttttca ttaactattt
     7501 tcacttgttg gtttttgact gttgcctcta gcccaactcc tgaaagattt tttagatttt
     7561 cggcttgata aggcttgatt tcatgttctt tcaaataatt cataatacca acagccaatg
     7621 gatgattggc attcgcttcc aatgctccta aatattgaag gatttcattt ttattgaacg
     7681 catcatcaag tacttcgaca cccgttacag taaattttcc ttctgttagt gttcctgttt
     7741 tgtccagcat gatcacatct agatcattgg cttgctccag tgcattgcgg tttttcaaaa
     7801 gtaatccgtt tttagctgct attgatgtag agcgagcaac aactaatggg atagctaaac
     7861 ctagagcatg aggacaggcg atgataaaga ctgttaccat acgttctaat gcattaggaa
     7921 gatctgtcaa gaacaaccag gcgatgaatg ccaggatacc cacaattaat gcgacataaa
     7981 acagccattt tgccactttg tcagataatg actctagctt ggatttttct ccttgtgctt
     8041 ttcgcaccat ttccataacc ttcgctagat aaccattttc accagttcca gttacttcga
     8101 tttcgatcgt tccatcccca ttgatagaac cgccaatcac tttgtcattg gcttgttttt
     8161 tgattcctct tgattcgcca gtcacagctg attcgtcaac aattgtttca cctttcaaga
     8221 ttacaccatc tgttggcatt ttatcaccag accggacgat caaacgatca ccttcgttta
     8281 cttctttcaa tgaaactgtc tcttcttttc catctttcgt caagcgtttt actgattctg
     8341 gcagtaattc tgctagtttt tgtaaagcat tgctggcatt cgacacagca ttcatttcaa
     8401 tccagtgacc tagtagcata ataacgatca aagtagccaa ttcccagaag aaatccatga
     8461 cgtgtgtatg tggattgagc agatttgcga taaacgaata gacactataa atatatgcaa
     8521 ctgtaatccc catcgcgatc agtgtcatca tagcaggact tttttgtttc aattccatct
     8581 tagctccact taaaaatggc tgaccgccat agataaatag tatagttgct aagatcaata
     8641 cgagccaatc agaaccagga aacgttactt gaaatggaaa ttccattccc atcattggtg
     8701 aaaaaagaat gattgggata gctaggataa gcgatagcca aaatttttgt ttgaaatttc
     8761 ccatgtgcat ggaatgatcc atgccgccat gatcgtgatc catgtgcatc gaatggtcca
     8821 tttgtttgct gtttgctata tctccatggt tatgtcccat gtgcatggaa tggtccatct
     8881 ggctatggtc atgtttcatc tccatctgtt tttcatgaga ttctgtactt tctttgttta
     8941 ctacttcttt tttatgttca tgttttttct ccatatcgtt cccattcatt tttactccat
     9001 cccttctctt actttttcat gtgccgattt aagctcaggg agttcaacaa tacactgacg
     9061 gaactaaacg ccatcgctcc tcctgcaatg attggattca aaaagcctag tgcggcaaaa
     9121 ggaataccaa tcgtattata gacaaaagcc cagaaaagat tttgtttgat ttttctcaat
     9181 gtcaccctag acaactcgat tgtttgtgca atggatgcca aactgctact catcagcgtc
     9241 acatcggctg tttccatcgc gatatccgtt ccacttccca ttgccatacc aacatcagct
     9301 aatgctaaag ccggtgcatc attgattcca tcgcctgcca taccgacttt tttgccatct
     9361 ttctgaagtt tttctacgta ggctgcttta tcctctggaa gtacttcagc aaagatatgc
     9421 tttggatcaa taccaacttg tttaccaatc gtttcggctg ccagtttgtt gtctcctgtc
     9481 agcataaaaa catctaatcc tttattctgg agctgcttga ttgcttgttt ggcttctagt
     9541 ttgatttgat cagccacggc aatcaacccg atcacttgct tttcatcagc taaaaacatg
     9601 actgtttttc cctgctgttc taattctaac gcgtgttctt ggtattcatc aaatgatagg
     9661 ttcaattcaa ttaatcgttt acgtgtaccc gcaaaatagc gtactccgtt aatcgtaccg
     9721 ctgattccag cacctgggtg agctgtaaaa tcaatcatat cgtatgttgc tgcttcttgc
     9781 agttttccgt attccacgat tgctttacct agtggatgtt cagaagcttg ttctaaagta
     9841 taaaaaatag ataggacttc tttagaaccg actagatctg taactttggg ctttccttct
     9901 gtaatcgtcc cagttttatc caaaacaatg ctgtctaagt gagcagctgc ttctaatgct
     9961 tctccgcctt taattaaaat tccatttcgt gcaccaacac ccgttcctac cataatagct
    10021 gttggagtcg ctaaacctaa tgcacatgga cacgcaatga ctaatacaga gacactatgg
    10081 atcaatgcga gttgccaatc accagtgatc aaacctgtag ctatgagcgt gataaacgcg
    10141 atgactaata cgatcggaac aaaaatccct gagatccgat ctgcgatttt ttggattggg
    10201 gctttgctcc cttgagcatc ttccaccatt tgaatgattt gcgctaatac tgtctgcttg
    10261 ccgacttgtg tgacttccat gtgtagcaga ccattcgtat tgattgtccc gccaaaaagc
    10321 gtgtcatccg gattcttttc tacaggaaga ttttcaccag taagcatact ttcatctatc
    10381 gcactgcttc ctgaaataat tcgaccatct gctggaatct gctcaccagg acggatgact
    10441 agttgatcac caacgactac ttcttcaatc gctaacgttt cttctttccc gtttcttatc
    10501 acttgggcgg tttttgtttg aagagacatc aattgtttga ttgcattacc tgttttcgtt
    10561 tttgctgtat gttccaaata tttgccgagc aaaatcagcg tgatgatcat actgctgctt
    10621 tcaaaataaa gatcttgggg gtgtccacgg aaaaatccgt tgtaaatgct caatgcaaag
    10681 gcagccgatg ttccaattgc taccaataca tccatatttg gtgctttcga cttgattgca
    10741 tggtacgcac ctttataaaa acgagcaccg acataaaatt ggacaggagc agacagcact
    10801 aactggacga ttggaaaatg aaaaaagtga acaatcgccg catggcttcc taacatcata
    10861 gcaatcattg acaacatcaa cggcaaagtc aaaaccgtac tcaatataag atcgaatagc
    10921 atttttttca aatacgcttg cttttcttct gcaatttttt gtttgtgggc ttcatcgtac
    10981 aaaattgcac catagccgat gttttctacg ctttggatta atttttcagt ggtcgtccct
    11041 tcatatttga cacttgcttt ttcagtggcc aagttgactg tggcattgac cacaccaggt
    11101 tgtccgttca gctctttttc gatcctagca gaacaatttg cacaagtcat acctgtaatg
    11161 acgaacgtat ccattttggt ttctgctgtt gccatcaaat cacctctgct ttataaccca
    11221 atccattgat tgcttgacaa atcttttcgg attgaacttt tgtttcatca aacttgaccg
    11281 taccgtttgc ttttttcaaa tttactttca ctttttgcac gccgtcaagt gcgcttactg
    11341 tttcttctac tcttgctgca caatgattgc agctcatacc ttcgattgaa aattgttgtt
    11401 tcattttttc ttcctcctat tgatgttgtt tacattcaca ttgacctggg atacagttac
    11461 attcgatcgt atctactggt tgcttttgtt ccagttgttt caaaatctca ttgatatctt
    11521 cttgagtcaa agtggcttct tctactagat ccgcaatcgt ctctccaatc ctttttgcac
    11581 agatatggga aaaaagattt tccgtcgcac tgcgaactgt ttccatctcc gagacagctg
    11641 gatgataaat gaattttttt ccttcttgtt ctgtccagat cacttctttt tttaccaaac
    11701 gacccagcaa agttttgatc gttgctggtt tccaatccat cgaatcagct aagatctgcg
    11761 tgatttcttg ggctgtcgtc tgtccaagtg tccaaataac acgcatgatc tcccactctg
    11821 aatcactaat ctttactggc gttttgactt tttccattta tttcacacct ttcgtttaca
    11881 aatgtaatca ttaacttatg aatacagatt acacttgtaa acaaaaactg tcaattattt
    11941 ttgctctcta ttttcaattt agttaaaaat tcttgtttct ctcgtcttgt tttaaggatg
    12001 atcagtttat ttcttgcatt agctacttgg atcagctgct gtatttttgg ttcattgttt
    12061 ccttcaaaat gccagataaa agataaaaat tctaagtatt tcctgtttag tttctccgaa
    12121 aatgattcag gcatatctgg ccgagatttt ttgtttatct ttctttttag agaacgccaa
    12181 actactcggt agatggcgag ataacgcggc actttaagcc aaatgatctt atctgcttct
    12241 ttaatccttt cctctaacgt agaagtataa tttccttcaa cgatccatga cggattagat
    12301 accataaacg attgctgttc ttgcaaaaac cacttttttg cctgaatgcg gaaagccggt
    12361 gatttctttg atcttccttg cgagggacga ttttcccgac ccactttgtc caatcacgat
    12421 gatcttcatt cttctccctc acttctcttt ggaatttggt tcgatcatac cacttataac
    12481 gccgcaaaaa aacaagggac tgtgacaaaa gtcgtgtcaa agtcccttgt ttcgaataaa
    12541 tggtgttcaa tcagctgacc ttcagcatta gctcaaaaaa cggcaaaata tgaggagcta
    12601 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    12661 cggtgttcca gaagtagctt ctgtcacaac ctcttgtaac aagtatttct attttaaggc
    12721 tgaaccactg tttctggttc agtaatttct ttgtttttgt ctcgacgagc aattgctggc
    12781 aagatcatac ctaataagat caagacaatt ggtgtgatga tattcgtgaa taattggaac
    12841 caccatgctt taggatcagc agtataatct acttttggca ccattcctag gatacatgca
    12901 aacgcagtga aggcaaagca ccaagcacca aagataaaac cgatttttgg atttttaaca
    12961 aatttatatt cagaattgaa tttcttataa gctttattca acatcatata tgccaagaac
    13021 acccataggt aacgcattgg catgacaact gagttcaagt tcgtcaacca ttttactaat
    13081 tcattcatat ctttgatacc aaagatcggc aacaaaataa tgattcctac tagtatacct
    13141 gtcagaagat aaccattcac caatgtacct ttacgattac gtttacgtag ccagtttggt
    13201 acaaattctg gatccgcatc tgctaacaag atttttagtg gtgcatcaat tgaaaaggct
    13261 aatgcggaga tttgtccaat catgtttgtc aatgcgtaaa tgatcatcaa tgagttaccg
    13321 ataccatagt aattacctaa aatttgaaaa gcactgtatg ccccatttgc catcaagtct
    13381 ttagggatat tatcgctagc aaacagcata cccattgcga ctgagcctaa tacagcacaa
    13441 atccctacca ttgctgctaa gaagatcatc cctttaggaa attcctttgc aggatttctt
    13501 gtagagttga cataagggga tattttttcc gcccctccaa ctgcaaatac tagcatcgaa
    13561 acggttgtga aataactgaa atcaaatgtt ggaatatatg ttttcacttg attcatatgt
    13621 ggtgtggcaa attccatcgt tggattgatg aacggtgccg ttacagctaa aagaataaat
    13681 aagatcgaca tgacgaacat cgctgttccg gccagtccgc caatcacttt caaagtagaa
    13741 agtccttttg ttgataaata taaaaaggca agaaatataa taagtgaaat aactgctacc
    13801 atttgtactg acatcgtatt tacgatattt ccatttcctt ggactgccca accaccagca
    13861 atcagaattg cttgaggctt ttgtgccaaa taaggaatat ggacgaccca gtaagtccaa
    13921 gcggcatagt atgccagtcg tttattagat gtcgtatttt cgacccatga actgacgcct
    13981 cctttactat ccttgaaggc tgatcctaat tgtccaacga tcaatgcgta tggaataaaa
    14041 taaatggcca aaatcaatag ccaagaggtg acaactgata ctccttgctg agcatagtta
    14101 ttaactacat tccccatgcc ccaaaccatg ttaaatgcaa ttaacgcaac agtaaaccag
    14161 cgtaattgtt tgttgttcat tcgttctctc ctttatgata aaaatttgtt atccagatgt
    14221 aatcatattc aatttttaac aaacttacaa gacagtgtta ttttatgtgt gtattctttt
    14281 atttaataca catcgttttt tcatattttt tccatcaaaa aaataagtta ttttctttta
    14341 tttcattgac cttattgaat ttcaagctat tttgaatttt ctgcaaaatt aaaaacatta
    14401 gttattttat aatcctatct attttcttta catttttcat taagaaaagg atataaaaaa
    14461 aagaggacgt tcattcgtcc tcttcattga tcaacgttta ttttttacat gcaagcttcg
    14521 catggcattc aaaacagcta atactgtcac cccaacatct gcaaatactg catctcccat
    14581 tgatgcaaaa ccaaaagcac ctaaggaaag taccaaaatt tttatcccaa tagcaaagac
    14641 aatgttctgt ttgacgattt tcaatgtttt ctttgctaaa tggattgctt ctgcaatctt
    14701 cgatggttga tcattcatga tgactacatc tgctgcttca attgcagcat cgcttcccaa
    14761 tccacccatt gcgattccta catctgctct tgctagcacc ggtgcatcgt tgattccatc
    14821 tccgacaaat gcggtctttt tattgttact ttgtaagatt tcctctaacc gctgcacttt
    14881 atcttctgga agcaattcgc tatatatctt gtccacgcct acttgcttcc cgatatgatc
    14941 agcaattttt ttcgagtcgc ccgtcaacat gactgtgttt ttcacaccag cctgttttaa
    15001 ttgaaccaat gcgtctacgg catcttcttt caaggtatct gcaataacaa gataaccact
    15061 gtaactttga tcgatcgcta gataaagcaa tgtaccgatt tcttgacttg aaggaaaaga
    15121 gatttcaaat ctttccatca atttgtgatt acctactaat acttgtttgc catcaacttc
    15181 agctaagatc ccttcacctg caaattcctg gatattagct gttggtaaaa gcggacctgc
    15241 gtagccttct aaaacagatt gagcaatcgg atgtgtagaa aactgttctg cacttgcaac
    15301 atattgcata aatttctttg gatcaattcc tgttggatcg atcgtctgga tcgaaaagtc
    15361 acctttcgtt aatgtgccag ttttatcgaa taccactgtc tcagtttgtg caagcaactc
    15421 caagtaattt gaacctttta ctaatacacc gattttactt gctcctccga taccgccaaa
    15481 gaaactaagt ggcacggaaa tcactaatgc acatggacat gagatcacta aaaatgtcaa
    15541 tgctcgatag atccattcag aaaacgccgc tctagaaaca acaagtggag gtaaaacagc
    15601 taataaaacg gccaatccta caacgactgg tgtgtaatag cgcgcgaatt tagtaatgaa
    15661 attttcagct ggtgcttttt tactgctggc attttctact aattctaata atttgctgat
    15721 agtagaattt tcataagatt tttctacttg aactaaaaat ggtttatttt tattgataaa
    15781 tccgcttaat actgtttcac caacatatac agatttaggg actgattctc ctgtaagtgc
    15841 tgacgtatcg actaatgatt ctccttcgat gatcgttcca tctaacggta cacgttcacc
    15901 aggcttcaca agtacaaact gaccgatcag cacgtcttca gggttcacgg tcatcaatcc
    15961 ttccgccgtc tgcacgtgag ctgtttctgg acggatcgcc atcaattcgc gaatcgattt
    16021 tcttgaatga ctgactgcca atccttggaa atactcgccg atttgataaa atagcatcac
    16081 tgctacggct tctggatatt caccgatcaa catagcgcca atcgtagcaa cagacataag
    16141 aaaattctca tcaaagattt ttcctttgac gatattcatc actgccttct tcactacatc
    16201 atatcctatc agcagataaa tcaataaata ccctatcaag cgaattggtt cattaaaatc
    16261 aaataatagc aataaagcga tacctgcaag ggaaaataaa atccggaaca gcgtatttct
    16321 ctttttttca tttccatgtt cagatacttt ttccttatct atttctgtta ctttgacatc
    16381 tggttctaat tccttcacta ctttttttgt ttcttgttca atcgtagcca aatgatcttc
    16441 ctctgcgtca atcgttagtt ttccagaagt gaagtttacc gttgcttcct tgactccatt
    16501 gattttctga actccttttt cgattttcat tgcacagttc gcacaatcta aaccttctaa
    16561 tctgtaactt ttcattttaa tgacctcctc gcctcaacat atgaatatct cttcataact
    16621 aattataaat gaatggttat tcatatgtca aggtatccga aagaaaaaat gaaaaacatc
    16681 tgcatcggaa aatttcttcc atacagatgt tttggcttat tctttctttt tctcttcgat
    16741 cattgccact gctcttatcc aacccgcact tgccttcttg atcttgacag gatagcaact
    16801 aagtttgaag cctgttgctg gcaatttgtc taaattcatt aatttttcga taggacaata
    16861 tcttttttca atccctgcaa aatgtccttc ccagatatac gaagctccac tacgctttca
    16921 aaaatataga aagagttctt taatgaaaat tatttcaact ttccataaac agtaacccca
    16981 tataaataaa agacgtaaaa gtcacataga agaacttata caggaaaacg catagagtca
    17041 ggtgaaatac atgtatccca cctggctcta tgctttatct aacctttatt attctctttt
    17101 atacaatttc tgcaccgaat ggcatcaacg ccaatcgagc aagtttgaaa gattgcttgg
    17161 caaaaggtat ccctatgatc gtgatacata gaaggatgcc gctgacgaca tgtgcgagag
    17221 caattggtag gcctgaaacg atcagccaga tgatattaac gatcaatgac atgccagact
    17281 ggtgataaat tacttctttg ccaaaaggcg caaaagaaag ggaggcgatc ttaaaacatt
    17341 gcataccgat tgggatacct atgatcgtga tacaccaaag tatccccgct gctaaccagc
    17401 taagaccacc gattagtccg ccgaatataa accagatgac atttcctaaa caactcattg
    17461 tatttctcct tctattactg tttttgatta aaactgggca aaaattgatt ttattcttct
    17521 ttttttagct tccagcatca agtattctgc aaattttttt tggatttctt ccgtattttc
    17581 ttttgttact aatgtgtgtt ggattagttt tcctgatggt cgctgatcca atcgttcttc
    17641 tgactgttgc acgtccgctt gcgcaaatcc gataatccgt ttttcttttg ttacagcaac
    17701 gagcaatagt ttattcttat catttaggtc agctgccgtt tttgctttta cgaatgattc
    17761 tggatgatca gtaatcagct gattgatttg aggtaaatct tcttttactg cccaccttat
    17821 ttccatcatt tttcactttt ctttcgctca tttttatctc cactaaaaaa atcgactgcg
    17881 acaattgcat ctatccagct ttgtgcaatg atctcatgtc cgccaatcgt tgggtggacc
    17941 ccatcttctc ttgtatattt cgaaaaacca taagcaatcc ctaaagcatt gaatatacca
    18001 tctaaaggaa ccagcaatgc gccatattcc ttagcaagtt tacgaacaat ttggatttta
    18061 gggtctaagt ctttacgcca gccatgacgg tcgtcggtta taggaagtac aaacggctcc
    18121 atcaagatga tgttgcagat ttccgctgtt tgaagttcat ttagtaattt tcgataaacg
    18181 gattcaaatt gctgagcaga tttctcagtt ccgaatatag cctgatcgct agtattatgc
    18241 caggtatcat tgattccgat caaaatcgat acatagtcag gttgtaaagc taaacaatct
    18301 tcttgccagc gtacttgcag atctgcaatt ttgtctcctc ctatcccacg gttcatgcat
    18361 tcaaattcca tcgttggata ttgggagatt agttttccgg caatcaaaag tggataacct
    18421 ttacctagat cagttgcttc tgctcggtat cttcctgcgt ctgtaatgct atcaccgata
    18481 aataatatac gttttttcat ttgtcaaaaa ctcctttttg aataaaacga tgattttctt
    18541 ccttatatta tcccataagc cctatcaaga cgaaagagaa aatatatgta taaaaaatga
    18601 caccagccta gttccttcaa acaattgaat gaacgctcat caagtgtcta gttttttatg
    18661 ctattttttt cttaacgtgc cggattcccc ttgattcctt cgttgtttgg tccagagaga
    18721 tcgatcacac ccaagcttcc gtcaatcaga ccactgatga cgccatcgaa acatatattt
    18781 ataggctcat ctaagacttc tagtaaatgt ccctttacta atatctcttc tagattgctt
    18841 gggatagatt ggtcataact ttatctggtc cgatgatttc aagggtgaat tgaacttaca
    18901 tatggattat tgcatgctga aaatacggac ctactccatc ttcgaatgtt actattagtt
    18961 tgttttcagg tgctagatgc tgctttattt caacagcaga agcatcatca aatcagattt
    19021 ccatttgaat taccttcttt gtaaatgttt gattgattgc gatttcgtta tagaaccttg
    19081 tgaatcaaat agcaatttct tttgctttct tctcttgtct gctggaaact tccattatcc
    19141 cgtatttaaa aaaaaagaac tgcgagagtt cgcagttctt ttttttaaat aaacgttgct
    19201 ctccggccac aagtcacaat agtcatcaaa catgaaaaac tgttttttga aattactcct
    19261 tgtgactcgg agctgaacgg caggcgcaca acctcttatg gataaattcg gttcaacaaa
    19321 cgtgggaatg gaatagcttc acgtacatgg tcgataccag caatccaagt gactgttcgt
    19381 tcaagaccta gaccaaaacc agagtgcgga acagaaccgt atttacgtag atcaagatac
    19441 catgaatatt ctttttcatc caaaccgtga ttacggattt gttctagcaa gaaatcatag
    19501 tccgttgccc gttcgcttcc gccaataatt tcaccgtaac cttctggtgc gatcatatct
    19561 gcacaaatta ccacgtcttc tcttgttgga tgtggtttca tgtagaatgg cttgatggct
    19621 tttggatagt ttaggataaa gacaggctgt tcgaatgaat tcgcaatgaa cgtttcatga
    19681 ggtgaaccaa agtcttcgcc ccattcgatg tcatcaaatc catttttctt cagtaattcg
    19741 attgcatcat cgtaagtaat acgagggaat ggcaattttg tataagtttt taagacttct
    19801 ttgtctcgtc ctagtacatc taatgcgtat tcgcaatgat ctaaaacatt ttgtactaag
    19861 aatgctacat attgttcctg tacttctaaa ctttcttctt gatgagtgaa agccatttct
    19921 ggttcgatca tccagaattc gatcaaatga cgtcgtgttt ttgatttttc agcacggaaa
    19981 gtcggaccga atgaaaagac ttttccgaaa gccatagcag cagcttccat gtataattgg
    20041 ccactttgag ataaataagc tttttgatca aagtaatttg tttcgaatag atccgtagtg
    20101 ccttctggtg cagaaccagt caagatcggc ggatcaattt tgacaaaatg atgttgatta
    20161 aagaattcat acgttgcacg aatgatttca tttctgatct gcataatcgc atgttgtctt
    20221 gaagaacgca gccataaatg acgatgatcc atcaagaagt ctgttccgtg ttcttttggt
    20281 gtgatcggat aatcgtggct ctcgcttacc acttccacat ttgtaattcc gatttcataa
    20341 ccaaattttg aacgactgtc ttcacggatt tctccagtta ctaacacgga tgtttcttgg
    20401 tgtagatttt tagctgtttg gaaaacttct tccgacactt cgctttttac aactacacct
    20461 tggaaaaagg ctgttccgtc tcttaattgt aaaaatgtga tttttccgct tgatcgctta
    20521 tttgcgaccc aagcgccgat tgttactgtt tctcccacat ggtttttcga atcgatgatt
    20581 tgaatttgtt ccacgtatga ctctcctttt atattccttg aatttcttca ggattaattt
    20641 tgacttttac tctcaataaa ttgcttgatt ctgcgtaccg cttcttctaa cgtagcgcga
    20701 tcagtagcat aactcatgcg gacattttct ggtgccccaa agccttcgcc agtcacaagc
    20761 gctacccctg tttcttctaa aagatcttct acccattgtg tgacattttc atatttacac
    20821 atgcgtagcg tttcttttac attaggaaaa agatagaaag ctccttgcgg cttttcaaga
    20881 gaaacacctg gcagttctgc cacaagcgga tataacgtat tcaggcgttc ttcaaaggcg
    20941 ttccgcattt cttctactgt atcttgctca cctttcaatg cttcaacagc tgcatattga
    21001 ctgactgctg tcggattact ggttgactgg gaagcaacag caatcattcc attgatgatc
    21061 tcagaattcc ccaccgcata accaatgcgc cagcctgtca tcgcatatgt tttagacacg
    21121 ccattgatga tgatggtctg atttttgatt gcttctgaaa tcgttgcaat tggagtaaac
    21181 tcattgccat tataaactaa acgaccataa atatcatcag caactatcaa aatatcattt
    21241 tttactgccc attcaccgat tgcctgaagt tcattcttgc tataaatcat tccagtcgga
    21301 ttcgatggag aattgatgat tacggctttt gttttttctg aacgtgcttg ttctagctgt
    21361 tcaacagtaa ctttccagct attggattct tcccctttca caaagacagg aagtccttct
    21421 gccagtttta cttgctcacc gtaactgacc cagtaaggca caggaatgat gacttcatct
    21481 tgcgggtcaa ggatcgtttg gaaaagggta tacaaagcaa atttcgctcc gtctgttacg
    21541 actgtttgtg aaggttcata gacaagtcca tagtttttct tcagataatc aacgattgcc
    21601 tgtcgaagct ctgggattcc tgctgttggt gtataatagc tagctttccc attgcggatt
    21661 gcttcgattg ctgcttcttg gatgttttct ggtgtcgcaa aatccggttc tcccacagtc
    21721 aaactcagca catctctgcc ttttgctttt aatgccttgg cctttgccgc agctgccaaa
    21781 gtgacagacg gttctaatcg ttttgttctt tttgataact ccatcccatt cattccttta
    21841 ccttttaaat tcccttgatt gtcttgactt catctccaga ttcaaagcca atcaggtaat
    21901 aattcagttt cccatttgtt tcttttccta ctacttccca cacaggctga tcatcataca
    21961 tccctaaagc agctttttcg atttggattt caggatgggc cgcagcaatt gtctgttttg
    22021 cttcgttttc agtcagtccc tttgtttgat ccaatacttt tactttttca ccagatttag
    22081 gaatgataac ggcgatttct tttcctttat catttgtacc tgtcagtgaa aagtatgtct
    22141 tctttcgagt aaaccaataa aaatgatcgg cttcctcaag atttgcatat ttctttgcga
    22201 tttctactgc ttctttcttc gcctgcaccg ccggacgact ggaacgcaag taaaaaatcg
    22261 agctgatcac gatgaccgct aataaaaata cgatcaagcc aagtaacacg cgattaatcg
    22321 ttttttcctt tgtctgtttt ttcttcaagg gtcgtcgctc ctttatcttc attccttttc
    22381 attatagcag attttaatta tgaaaaaaga cagctcctct aacttccttc ttcatttttc
    22441 tttaagaatt catggatgtc ctgatagatt tcctccatag ctagctcttt gatcggtact
    22501 tttttcggta atgcatggat gatccgtgca ccatattttg cagtgactag tcttcgatcc
    22561 aaaataagca atacgccacg atctgtctcc gtccttatca agcgacctaa tgcttggcgt
    22621 aacttcaaag ctgctttagg tacggattcc tggtaaaaag gattgatccc ctctgattct
    22681 aaataagcgt tcctagcttt gaccatcggg cgaagcggat tatcgaaggg taaacgtgtg
    22741 actacaacga tctgcaaagc atccccaggc aaatctacac cttcccaaaa gctgtcagcg
    22801 ccaaacaata cgcttttttc tgacaaaatg aagcgtttca aaagcttctc ccggcttccg
    22861 ccaaaccctt gagctagtat ttctcgtcct ttttctagca tagacagatg cattttctga
    22921 tagaccttct gcaaaacatc atgagaagtg aataacacaa gtattggccg atcttcttgt
    22981 tcgatcagac ctgtaagtgc tgtctctaaa taatcgatat atgcttgatt gctgacttga
    23041 tggacggcag gtgcctcttt tggcacatag atccttgcct gtttctcata atcataagga
    23101 gaggcaatca ctttaagagg tgctgcttca atgccaagcc gcttagagag atagtgtcga
    23161 ttagggccga tcttcagcgt tcctccaata taaagaatac gatcatatcg aggataccaa
    23221 acagtcgacg gcaacatagc cgcttgaaaa tcatggagat gaaaaatcat ctgctgcgct
    23281 gtgttcccag gaacgaacca atggatcaaa tgatctcgcc agtcttccag ccattgatcc
    23341 atcagcaagt actgcttgtc taattgatca aataagctat ataaacttgc taaatcgatc
    23401 cgcatctggt ttgtccagct ttcttggata atctcaaaag tcagacgcat ttgtgactgt
    23461 agctgctgga tctcctgata aaaaagctgc aggtgatcaa tggctgtccc agattccagt
    23521 ggcagctgat ccaacatctc ttttgtcatc atgacttctt ctggatactg tccgttaggt
    23581 tgcttgccgc tcactaaact cattaatcct tctgccatac aaatctgtgc ttcgcatagc
    23641 gcagaaagtt cttctcgata aatcgataac cagcgtaagg cttcatgttc tttcggtaaa
    23701 tagctttgta ttcggtcaaa cagctgattt ggttcttcta attgctggat ttgtttatgg
    23761 aaatagttaa tacttaatcg tttgtcattt actttttcag cgatttccgg caaatgatgt
    23821 acttcatcga tcaacaaaaa tggactagct ggaagcaatg gttcttttcg ctgtgtttct
    23881 tgtattaaaa acgcatgatt gacaatcaaa aaattacttt gcctgatttt cttttgcaag
    23941 aaacgaacaa agtcttgttc gtaaaatgac tgatttcttg ctaaaaatcc aatacctctg
    24001 tgcgcgatat ctgcaaagaa tgaatggttc aaatttgtta aatgtaattc atccaaatca
    24061 ccggtcttcg tttgcgtcag ccaaactaag accgccattt gatacaaagc atattgtttt
    24121 tgttctggcg gttccacaag tgtgccttta aaacgctgta aatcaatata gtggcgatag
    24181 cttttgataa ttacaggttg tatgggctga tctagcaatt gattcagcaa tggaatgtct
    24241 ttttctatga tctgctgctg caaaactagc gaaacggtac tgatgacagc gggtttttca
    24301 ggtgtagcca aataactcaa tggcagcaga taaccgattg ttttacccat tcctgtagct
    24361 gcttcgatca tcaaatcttt tgattcatct tttgtgaaat ggtcgtagac cgcattcatc
    24421 agtctgttct gttcttttcg atacatcagt gttttaccaa acattttttc ttttgcttgt
    24481 ttttttcttg gatatgtccg ctcgccataa tggacggatg tgaatagttc cacttctttt
    24541 ttctgcaaag ctagcccatc gacgatttct aatgagggat caagtggctc tggtttttct
    24601 ttcatttctt caatcacatg ataaatgaaa cgactcgtat ccatacctgt gtgtctactt
    24661 aatcgcgcaa tctgctctaa cgtgatgatt ggcaattttc tcatccgttc ttcgatcagc
    24721 aaaagaagtt gacccgtcac ttcggcatca ctatctgcct gatgaggatt ctcatgaata
    24781 aatccaaggc tttctgacaa atcagctaag cgaaagcttg gttccgtcgg caagaaaatt
    24841 tgtgcaagtt ctactgtatc gatgcctggt atcttcaaac taggaagtcc gcatcgcatg
    24901 agttcatggt tcaaaaaatt ataatcaaaa taaatattat gcgcgacaaa tatggtgtct
    24961 gccaaaagat tgtaaatcgt ttgggcgaca tcttcgaaat aaggcgcttt ttggacgcgt
    25021 tgattcgtga tccctgttaa atgctggatt tgttttgaaa tcttccgccc tggatgaata
    25081 tcgatcgaaa aacgtgaagt gatccgacct gattcaatca tgacgcagcc aaattggatg
    25141 atccgatctt cttttggatt cgtcccagtc gtttcgatat caacgactgc ataagttctt
    25201 ggtttcaaaa tatcttcctc ttctcctgcc tctaatgttc ttttgaaatt atactacaaa
    25261 gtgcagaaaa atactctcat cgctttttaa agaaaataga caaaaacgcc cagacacgca
    25321 aatgtctggg cgtttatttt ttagtatagg ttaattaagc tttagtaacg tttgttgctt
    25381 gaggtccgcg ttggccttct tcaacatcga aagttactgc ttggccttct tctaaagttt
    25441 tgaatccgtc gccttggata gctgagaaat gaacgaatac atcgtttccg ttttctgcag
    25501 tgatgaatcc aaatcctttt tctgagttaa accattttac tgtacctgtt tccataataa
    25561 aaatcctcct tgtgtatcaa cacatatttt gcaattactt gaatggtgta tatctggaag
    25621 atgtttattt gaaacaagct accacagatt accgaacaaa aactactcct atagtatatc
    25681 ataccgttta caaaaaagct agagtttttc gacttttttt attaaaaaaa gttttctcag
    25741 cgattacata aagtgaaaaa ccgaactttt tttaaaataa acgacatatt gttcagttgg
    25801 tgtttttttg ctctgatgtt cttctttaaa ctaaaaaaat tattctttct gactacttat
    25861 cggttctgtt tcaacggtcg aggctgggga agattcttta gtaggtctga tgttctcatg
    25921 gtatttctga tagcctatga cagttaccag aagaacaagg caaaccaata cgatcaacga
    25981 gattagtttt tcttttttgc tcatatctat acctcataaa taacaggatg atgtccatca
    26041 tatttctgag tatgagtaaa agcaaaagct gcagctgtag aatgtaagaa atgccagcaa
    26101 ctttcataat cacaggtcgg acagtaccaa aagtaaccaa atcttggctg caaagaccgt
    26161 tcttctgttt caggaatcgc agaatcaaga tctgttgtct gtacataaac atttttgtac
    26221 agacgtgtgc ctgtaatagg agaccgctgt gtttcttcag caaaaactgg ctgacttgtt
    26281 aaacttaggg atagaaacag caaacaccat aacagtcttt ttttcataaa gacctcctta
    26341 tcgataagtt tttcccaatt aaaatcatat ctcaagaacg cccgttattc acattttgtt
    26401 cataaatgat gttttttgta tcataatcgt caaaaagagg tcgtgaccac gtcacaacct
    26461 cttttt
//
LOCUS       DACDYI010000044        26352 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.044, whole genome shotgun sequence.
ACCESSION   DACDYI010000044 DACDYI010000000
VERSION     DACDYI010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.044"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(126..1328)
                     /locus_tag="IWM52_001701"
     CDS             complement(126..1328)
                     /locus_tag="IWM52_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9973951.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            1445..1960
                     /locus_tag="IWM52_001702"
     CDS             1445..1960
                     /locus_tag="IWM52_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9973952.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(2159..2605)
                     /locus_tag="IWM52_001703"
     CDS             complement(2159..2605)
                     /locus_tag="IWM52_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP9973953.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2652..4130)
                     /gene="msr"
                     /locus_tag="IWM52_001704"
     CDS             complement(2652..4130)
                     /gene="msr"
                     /locus_tag="IWM52_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP9973954.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(4484..5113)
                     /locus_tag="IWM52_001705"
     CDS             complement(4484..5113)
                     /locus_tag="IWM52_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP9973955.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(5097..5561)
                     /locus_tag="IWM52_001706"
     CDS             complement(5097..5561)
                     /locus_tag="IWM52_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9973956.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(6048..7316)
                     /locus_tag="IWM52_001707"
     CDS             complement(6048..7316)
                     /locus_tag="IWM52_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP9973957.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(7547..8284)
                     /locus_tag="IWM52_001708"
     CDS             complement(7547..8284)
                     /locus_tag="IWM52_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9973958.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(8351..8920)
                     /locus_tag="IWM52_001709"
     CDS             complement(8351..8920)
                     /locus_tag="IWM52_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP9973959.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            9048..9920
                     /locus_tag="IWM52_001710"
     CDS             9048..9920
                     /locus_tag="IWM52_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9973960.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            10262..11134
                     /locus_tag="IWM52_001711"
     CDS             10262..11134
                     /locus_tag="IWM52_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9973961.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            11131..13089
                     /locus_tag="IWM52_001712"
     CDS             11131..13089
                     /locus_tag="IWM52_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9973962.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(13421..14272)
                     /locus_tag="IWM52_001713"
     CDS             complement(13421..14272)
                     /locus_tag="IWM52_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9973963.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            14395..14862
                     /locus_tag="IWM52_001714"
     CDS             14395..14862
                     /locus_tag="IWM52_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAP9973964.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(15125..15607)
                     /locus_tag="IWM52_001715"
     CDS             complement(15125..15607)
                     /locus_tag="IWM52_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9973965.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(15707..16570)
                     /locus_tag="IWM52_001716"
     CDS             complement(15707..16570)
                     /locus_tag="IWM52_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9973966.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            17168..18043
                     /locus_tag="IWM52_001717"
     CDS             17168..18043
                     /locus_tag="IWM52_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973967.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(18188..18340)
                     /locus_tag="IWM52_001718"
                     /pseudo
     CDS             complement(18188..18340)
                     /locus_tag="IWM52_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(18608..19939)
                     /locus_tag="IWM52_001719"
     CDS             complement(18608..19939)
                     /locus_tag="IWM52_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9973968.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(20088..20801)
                     /locus_tag="IWM52_001720"
     CDS             complement(20088..20801)
                     /locus_tag="IWM52_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9973969.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            20976..22400
                     /locus_tag="IWM52_001721"
     CDS             20976..22400
                     /locus_tag="IWM52_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9973970.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            22699..23406
                     /locus_tag="IWM52_001722"
     CDS             22699..23406
                     /locus_tag="IWM52_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAP9973971.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            23393..23716
                     /locus_tag="IWM52_001723"
     CDS             23393..23716
                     /locus_tag="IWM52_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP9973972.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(23805..24842)
                     /locus_tag="IWM52_001724"
     CDS             complement(23805..24842)
                     /locus_tag="IWM52_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9973973.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(25163..26146)
                     /locus_tag="IWM52_001725"
     CDS             complement(25163..26146)
                     /locus_tag="IWM52_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAP9973974.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
BASE COUNT         8009 a         5312 c         4620 g         8411 t
ORIGIN      
        1 aaacggtgtt caaaagcttg catctgcggt aataagccca accaaacgaa aaatatgaga
       61 agctattttt gtttggctgt tcttatgact tgatgcagga cagctttttc acaacctctg
      121 atttcttatt cttcttcatt tgtagcaaaa taattacggt gataaatgat acaacataca
      181 ctgataagaa caatggcacc tgcaaaataa aacgcatagc tttcaccaat atgttcacca
      241 atcacacctg caataaatgg tgctaaagct ccaccgaaaa agcggacaaa actatagctg
      301 gacgaagcaa cgtttcgttg cagacctggt atttccatag caatcgtcgt caacagtgta
      361 ttgataattc cttggaaaaa tcctgctaac acaatgctga tcggtacaag gactggcata
      421 gaagttccag ctcctaataa cagcaaacaa ataatgaata agacaaatgc tgctaaaatt
      481 gtccgataag tcgtaaactt ccgctcaaga gccggtgcaa cgaaaatcga agcaatagcc
      541 agaagcacac cccagccaaa aaatacaaaa ccgacttcta attcagaata gccaatcaat
      601 aaaaatggcg catacgcaag taaagtaaag aatccgaaat tatataacaa tgcgattagt
      661 ccaactgaac gaagtttatg attgcttaac gctttgacac cttcccaaag gcctactttt
      721 ctctttggtt tctcagtttc aggtaaaagt accgctaggg ctataaaacc gcaaaacatc
      781 aaaaatgcta ccccaaaaaa tggataacgc catgaaccac tgcccaagac cccaccgact
      841 agagggccaa cagccattcc gcagccaagt gctgcttcat acatcatgat tgctttttct
      901 gtgttaccaa ctaaaatact gacgatcgtt gctaaagcag tagagacaaa taaagcattc
      961 ccgacacccc agccggcacg taacccaatc agcattccga tattcccaga taatccgcca
     1021 agaccagcga atacaacgat gataaacaat cctgtcatca atgttttttt caaaccgatc
     1081 cgactagata agaaacctgt aaatagcatc accacaccag tgactaacat gtaactagta
     1141 aataacaggg tcgtttcagc tggcgtagcg tttaactgtg cagcaatcgt ttttaagatt
     1201 gggtctacta gacccactcc cataaaagcg atcatacagg tagctgctac aactagcgct
     1261 gaacttttcc cctttgtttc ttcatttgtt tgttcttgta tatctatttg attctccaat
     1321 atttccatcc ctttctctct attagcatca tacatgattt attttacatg tcaatatgca
     1381 catgtaaaac gtggaaactt tactgtgata taataaagaa aataagacga gaggtaggat
     1441 ttttatgaat actttagcct atatattatt atgcgtgctt ttatccaaac cacgttctgg
     1501 gtatgaattg aagcagctga tcacaatttt ttgggaagcc catcacagtc agatctatac
     1561 aacgcttgcc aagttagacc agcagggtta tgtgaccgtt ttggacaaag acgagcactc
     1621 tcaaaaaaag atctaccact tgactgatga aggacgtgct cttgtagagg aatggatcaa
     1681 agaggaaact cccgctccta cgcaaaaaga tgaatttcta gcaaaaatct atgctttttc
     1741 gacgctcgat aaaacaacag ctactggact gctattcact agagaacaac agctgaacaa
     1801 aatcatgcaa aaaaatcaaa aaaaattgaa tgacctctcg ctagacaaga aacaagaatt
     1861 tggacgttat gttgtgatcc agcgcagaat tatgctttgt cagcaggaac tccaatggtg
     1921 tcagcaagta agagaacaaa tgcagcagtt ttttgagtaa gggataaaca gaacctttct
     1981 agaaatatct attgaagagc aaattatctt caataaaaga gcaatacagt tggaaaagaa
     2041 aaagcgatcc gaacgacagg ccaaaaccta tcgttcggat cactattttt ctagaactgc
     2101 attcaagaga ctatgacaaa aagcatgcca aggccttttt tcaaataaca gtgttccatc
     2161 attcattact ttgaattgct tttagcacac ttcttgaaag cgggccaacg atgatcaact
     2221 gcaacggtaa agcggcaatc acattcattt tccaagcatt catataagca gagaataatt
     2281 gatcaggtat tccttgttcc atgatcacac cgaataaaga catacatgtc accattccta
     2341 aaaccatcaa gcatgaaata gttaagatca tttggacctt gctttctgga tttactggta
     2401 gtttaaaggc aatttttttc gccagcacac cgatgatgaa gacatccaaa atgaaagcca
     2461 ccacgaatcc aggcactaga ccaatcaaca aacttgataa atgaagatct tgatgcagcc
     2521 ataaattata cgcgctcatc cccaagacca tcaaaaaaca catcattgtt gtaaaaatta
     2581 ttccttcttt tttatttgta ggcaatctgt accactcctt taaatttgtc acgaaaatca
     2641 tcgtagcaca tttaaaaatt tctcgtaagt acttttttta ttttctgccc ttttatttca
     2701 taaacttcat cagccgtacg ctcgacaaag taagaatcat gagaagtaaa caaaatcgtt
     2761 cccggatata tttgcattag cttctctaaa gcttcgatcg ttgccatatc aataaaatta
     2821 gtcggttcat ctaacagcaa cacattactt ggcttagtaa aaagcaaagc aatcgtcaga
     2881 cgggtcgctt ctcccccact taatgtacat aacggtttcg tcaaagtttc ctgagcaaac
     2941 cctaagtgat tcagcaaact acggacaagc gattctgaaa actctgtttg tctcatgaca
     3001 aaacgaatga tggattcttc agatgtcatt tgataaccct tttgctgata tacttgaaag
     3061 cttactttcg gagagagaag gattccttct ccttgtttgc ggatatgttc taataaggtt
     3121 gtcttacctg aaccattttc accgacaatc gcgatttttt tacctaaaga aaactgaaaa
     3181 tcaccattta ccagtaatgt tctcccactt ctttccaatt ggacattttg tcccatgatg
     3241 ggatagcggc tgtggagttc aagagatttt ggtactggaa aacggatttg tttcatctgc
     3301 tgcggcttgg ttgtttctcc tattttttgg agtcttctct ctaatgtttt cgcattcttt
     3361 tggatggctt tttgtacact gtctttttgt ttagaggaag acaaccgatc cagtctgatt
     3421 tgttgctttt tctttttact ggacactttt tccaactttt ccgcttgttt tttcttttct
     3481 tggatcgatt tttccaatcg ttttttctct ttcatgatcg actctgcttc tcgtagctga
     3541 gtctttttct ccaattcctt ttgagtgaga taggcactat aattccccga aaactctcgg
     3601 atttctccgt cttttacttc ccagattttc gatgctagct catctagaaa atagcgatca
     3661 tgactaacaa aaaccagcgt accataataa taacgaagct cttcaatcag tcgatcaata
     3721 ctttttctat ctagatgggt tgttggctcg tctagaagca tcccttgttc ataagaagaa
     3781 agaagacgta tcaactcgat ctttcgttct tcccctccgc tccatttccg ctctgaagtt
     3841 tcttccagtc ttaactggaa atgactggct aattccaaat ttaggtcctt tgcttcagcg
     3901 agataggtta attgaggcag gtagttgaag gtgatctctg tttggatttt cccatgatcg
     3961 ggaaaaagtt caccggctat tagtttgagc aaagtcgatt taccgacacc attttctcct
     4021 atgatcccta tacgatcttg ctgatagaca cgaagagaat caatagatag ttctagctgg
     4081 ttgccaaagc tgacttgtag atttgttata tttactgcta aattttccat atgttttaca
     4141 ctcctatttc atgtaaaaca acaattatcg atactaaaaa agagcagaaa aatctgtttg
     4201 caaaaaaact gcatcacatc ttttcctact ctgaacgaaa taactatgac taaatcacca
     4261 aatctgtcat agctttcacc tattctactg tcgttcgaaa aaatggacat actccatcca
     4321 tgaaattgtt gtttatacat gtcttttgaa tttatttagt tgttattcaa aagttcgaaa
     4381 cgtaatttca tcgatgcagt catttttccg acctcctttt ttattaggtt accacacacg
     4441 attcaaaaaa agcaatagct actgaatagt aactgaaaac cttttattcg aacatagtga
     4501 tcttttctgg tgctaaacga acagactcgt aaccttcttc taccgctttt cctattgata
     4561 aaataataac tggaacataa cggtctttat ccaaaccaaa tgcctctgcc aactgatctg
     4621 cttcgaatcc accaattgga tttgtttcat agccatgatc acgcgcaact aacatgaatt
     4681 gcatagccgc taagctagaa tcgactttga ctacatcgtt catttcttct cttgaaaaac
     4741 tcttgtaata aggaatgatc gcagctaatt gttgatctct gacttctttt ggcatcttcc
     4801 ctgattcgta agcttggttg tagatttctt cgccatattc gtaacattgc atatcgccaa
     4861 aaatcatgac cattgcagaa gaagtatcat tttgtctcgt attgaaacgg atcaaaggac
     4921 gcaagatttc ttttgcttcc tcgctttcag cgaccacaaa gcgccatggt ttcatattga
     4981 cagaagaagg tgcagtcgtc gcttctttga tcatttccag catttcttcg tgtgggattt
     5041 tgtatgtttc atcatatacg cgaacggatc ttctaccagt cataattgtt gaaaaatcat
     5101 ttttttgttc cattattatt aacctctttc gtttctgttg ttatattgca aagttgggta
     5161 ttcaatttat ctaaaagtgt tagaaattgt ttcatttcta catcgctgaa agccggggat
     5221 agcacggaac ctgtatcttt atggtccatt tcacaatgct tgattttctt ctgggctttt
     5281 tctgttagcg ctacatacac ttcccggttg ttctgaggat ttctctgacg cgaaacatag
     5341 ccatttgctt ccaatacctt cagatgacga gtaactgctc cttgatcaat ctgcaacttt
     5401 tcttgcaaac tgctttgtag caatggattt tcttctttca gtctcatcat gatctcgtat
     5461 ctagtaagac ttataccgat attttcttca aagatctgga cgattttttg atcggctaat
     5521 ttgatttgat ataaaaggtg actaatctct tctaaagaca taatttcctc ctttctaata
     5581 attgactcgt caacgattga cttgtcaata atagaataaa aaaagaaaaa agtcaaagaa
     5641 tcagctgtgt aataaaaaaa acgctctcaa tagaaaagta tagagaaaag aaattttttg
     5701 tagaatgaac ggaaaaaatg ataaagaaag cacaacgcta aaaccaagat tattgattct
     5761 tatcaaacta tagaagatag ctcttctgca gtagataatt ttacaccgcc aaaaatttcc
     5821 gaaatcgcct tttgcaagaa tcccgtcgaa tattagaaat tggatcagta tgcttaaaca
     5881 gtaataaaaa agttatgaca agatgttgtc acaacctttt ttgagtaaac ggtgttcaaa
     5941 aacttgcatc tgcggtaata agttcaacca aacaaaaaac atgaaaatct gtttttgttt
     6001 ggctgttctt actacttgat gcagggcagc tttttcacta cctcttttta ataaccataa
     6061 aatcctattc cttcatatct ccagcccatc ggtgttagcc aatctctttc cttagcactc
     6121 aaggtatagt gatgggtacc agttttttca tttggattaa atagtcgata aattggcttg
     6181 gaaccaccag aataaaaact tagaccttca taacgccaac cgatttttgt taaagagttg
     6241 atctcagatt gtgccattgt gtaatgatgg tctccactgt aaggattgta taaacggtag
     6301 acaggttgtc cgctgtttgg tgcttgccaa gcaatccctt catattccca gcctacagtt
     6361 ggcagataat ttttttctcc ggcattcaat gtatataaat gttctcctgt atgacggtta
     6421 tacagacgat acatcggaga agttgaagtt ggtgctgtat catactgtgt cagattatag
     6481 gtttcgatta cattgttcaa tgtagaacca taattaggcg cagtggcata acgacctgtt
     6541 agccaagccg tagcatcttt gtaagagttc gtattgcttt tccatgcacc ggaatagtag
     6601 taaacgcctg gctgaaaaga agtcgtcttc aataccgtta cgttatcttg caatgattct
     6661 ttgtacgaag gatattttct aaaagcggca ttcaccgtaa tccattgccc attcacatat
     6721 tccgaagtag gcatcgtaac agattggcca ttatagcttc ctttaatccc aaatagattg
     6781 taattagggg cagaagccaa agtactgttc ccccagccac tttcaacgat ggcttgagca
     6841 atcatgactg atgcatataa gtcattggca cttgccaaac tttgtgcagt tgaggcaata
     6901 ctatcaatga acgcttgttg agaattagca gatgagcgag cataaaaagc atagccagta
     6961 tagttttcga attcagacaa atctgcttga tgggtatgtg tcaatgtttg cccatctgtc
     7021 aattggttcg tgatttcttc tgcattctct ggattgttct gagcttctgc tgatgtactg
     7081 tcttcggttt gtgtttgttc tgttgtttca gttgttggtt cttcggttga aattgtattt
     7141 tctgagacag tagctgactg caaagtactt tctgtatcgt tttcaattcc tgtttctgaa
     7201 gaactcgatg tctgagttgc gacagtttct gtcgattctt cagctagcac tgttgtgttg
     7261 gcaactgcta gatttcctaa aagaataacg ctaaataata aattctttgc tttcatcttt
     7321 tttctccttt gaattatgaa acttttccta aataacagtg taactaaaaa agtaaaagca
     7381 agtcaatgtc catatttaaa taacatttgt ttctttttaa taaatttgct ttttctgcat
     7441 aaaaagacgt aaatgaagaa tattcttctc cattcacgcc ttaaattgat ggtcgtttga
     7501 ataactattt aacaagtaat tctctctgga tattatccgt tatggattat acttcctgtg
     7561 tcgtcggacg gataatcatg ttgctgacag acatccggtc tggcgtttct atcgcaaatg
     7621 ctacagcaga agctatatct tcagatgtta atccccattc tttttgcgct tcatgaagtt
     7681 caagagcagc ctttttgtcg ctgatggttt gatataattc agtttgaaca gctcctggag
     7741 agatgatcgt agatttgatg ttattttgcc gttgctcttg acgtaatcct tccatgattg
     7801 cacggacagc aaattttgtt ccgcaataaa cggcggaatc aggataaaca acatgtcccg
     7861 ccacagaatc agttgaaatg atatgcccag atttttgttt ttccatgatt ggaagtacgg
     7921 cggaaattcc atttaacaca cccatgatgt tgatatcaag catattttgc cattcatcac
     7981 ggtgaccttc aactaatgga gcggttggca tgattcctgc attattgtac aatacatcga
     8041 ttcgaccgaa cttttccatc gctaaatcga taactgcttg gacttgtgca aaatctctta
     8101 catctgctgt ttgaatatat agctcagctt ctggtaaaga ttcttttatt gcttttaggc
     8161 gatcttctcg gcgtgcagct ataactagtt tagccccttt ttctgctaat agcttcgttg
     8221 ttgcttcacc gattccgctt gaagcgccca tgatgacaat cactttttct gataaatcat
     8281 tcattctatt ctcctcctgt tttgaaaaat ctcttgctta tactcactat ctcttatttc
     8341 atcttactgt tcaaataatc cgttcgacag gtttgacgat tttggcaggg attcctgcaa
     8401 cgatggtatt gtctgggaca tctttcgtaa cagttgcatt cgctgcaaca atggcattct
     8461 cgccaatcgt tacgcctggc agaatagttg ctcctgctcc gatccaagca tttttcttga
     8521 tgtggattgg ctttactgac aagcctcttc gttcttttgg atcggttaga tggttcaccg
     8581 tgatgaggtt gacacgagga ccaatcaata ctgaatcttc taaacagatg ccacctaagt
     8641 caacgaacat cacttgacga ttgataaaga catttttccc tatactaata tgtttgccga
     8701 aatctgtata aaaaggcagc agaacagtca ccgtatcatc tactttgtca ctaatgatat
     8761 tttctagccg tttgcggata tctgctggtt catggtaccc agtattcagc gctgctaaag
     8821 gctcttcgtt ttccgcttgg atctgatgga tcacttcaaa gagcggatcc gttactcgaa
     8881 tctctttatt gacgatttgt ttttcgatga ggcttgtcat tttgctccct ccttttatcc
     8941 ttactttata acggacggct tatgatgtct aatacttata aaacatactt aactattcct
     9001 tttaactata cttagtggta tgctttttta aaaacaggag ggaaaaaatg gatattcgta
     9061 tactgacgta ttttttagca gtagctagag aaaaaaatat cagccgagca gcaaaatctc
     9121 tgcatatcac gcaacctact ctctcaaaac aactgaaaga tttagaagaa gaacttggtg
     9181 tgacactttt cactcgaggc tcccgagaga tacagttgac ggattctggt cgatatcttt
     9241 attcaaaagg aaaagaaatc ttgtcgttgg tagaaaaaac ggcgaacaat ttaacaaatg
     9301 acgcaatcat ctctggagaa atctacattg gcggaggcga aactaaagca atctctttca
     9361 tttctgatag ccttcattcg ttgatcaagg cgcatcctga tatccagttt catttatata
     9421 gcgggaatgc agatgatatc atagacaaac tggatagcgg attacttgat tttggactcg
     9481 taatcgaacc gacaaacaaa caaaaatacc agtcatttcg actgccagcc caagatcgtt
     9541 ggggactttt gatacatcgg aatcatcctt tagcaaagca aacagtcatc catccgcagg
     9601 aactgaaaac tgttcctttg atcatttccc ggcaaacgtc agtggacagt caactagctg
     9661 aatggctagg tttcagcata gatcagttat cgatcgtcgg aacatataac ttattgtaca
     9721 atgcttcttt gatggtcgat aacgggactt ttggtgcatt agctctagat gggatcatca
     9781 atacttatgg gacggaaaaa gtattcatcc cactcgatcc gccattacaa gcttcactga
     9841 atctcatttg gaaaaaagac cagcctcttt ctcctgctgc tcgtaaattt ttagatgtcc
     9901 taacggagaa atacagttaa gtagagtttt tttccttcgc tttcagcttt ttaacaacga
     9961 ataagttaag caaaagatcc aatgattaaa taacataagt ttcttaattt gttccttcct
    10021 tttatttctg ataattccag taaattacta taattataaa aaaagaattg acaaaacata
    10081 actgtctata taaaattgat tacgttaaca ataattcgtt tcatgtgact agatcatact
    10141 aggcatgaaa aatctctgag gaacatggtt tccctaggag gtttttcgtg tctatttttg
    10201 tttggccgca atcaactggt ctagcaaacg tgaaaaccaa atcttcaaaa agggtgcgcg
    10261 catgaaaatc aaaaagatct ttaatcaaaa tgcagtattg gtagatgacg gtggacagga
    10321 aaaagtagct attggaaaag ggattggatt tgacagaaaa agaaatgatt tgatttttga
    10381 tcgtgatgtc gaacggatat ttgtcatgga accagaaggt caaatcaagt tgcaaaattt
    10441 acttaatcag attgatgaac gctttttatt tgcagcagaa acgatcattg atcatgcgga
    10501 aactgttttg atggaaaagt tgaatgaaca tgtattgatc gctttgaccg accacattgc
    10561 tttttctgcg gagaacatta ataacggtat cgtcatccgc aacaaacttt taagagaaat
    10621 cgaagtgctt tacagtgatg aattcagtat tgctcaatgg gcagtagagt atctgacaaa
    10681 agagcttggg attccatata cttatgacga agcaggatat atcgcaatcc atattcacag
    10741 cggacgaagc ggtcggaaca ataaccatcg cagtatccgt gaagtgacga ttgtatctga
    10801 catcatccat ttagtggaat cagaattgga aacagatatc cattcagaac cattttcact
    10861 gaattattca agactggtga atcatttacg actgcttcta caacgaaccc atgcgcaaca
    10921 atatgctgtt ttagacacgg aaatcgtaga aatggtcaaa cgaaaatacc cagaaagcta
    10981 taagatttct aaaaaaatcc gtgtgttgct gaccaaagaa taccagctcg cgatcacgaa
    11041 agaagaactg ggttacttgg ccatccatat cgagcgttta agaagcgcaa tcgtacaaac
    11101 aaatgttaac aatcatgagg aggaaaaaaa ttgaaagcat atctacaacg aatgggacgt
    11161 tcccttcaac ttccagttgc ggtcttacct gctgctgctt tactagtagg gatcggaaac
    11221 tggtgggcat cttatagtaa cgacattgtc gctcattttt tacaagctgg cggtaacgca
    11281 gttttaggtc aattaccttt gctgtttgcc gttgggttag cactagggat gtcagtggac
    11341 aaatcaggtg ctgctgcctt ggcaggtttg gtcgcatttg aagttccagt aaatgtttta
    11401 aaaactgatt cagtagctac tttattgaat atcaaaccag aatcagttga tccatctttt
    11461 gctcagatca gtaacgtatt tattggtatt attgccggat tgattgcggc agctctttat
    11521 aatcgtttcc acgaagcaaa attaccgatg gctttatctt tctttagcgg caagcgtcta
    11581 gtgccaatct tggctgcctt tgtcatgttg ctgatcagtg cagtgctact gctcgtatgg
    11641 cctccggttt actctgcttt agtttctttt gctaaattca ttttgagttt aggctggggt
    11701 ggtgcaggtc tttatggctt cttcaaccgt ttactgatcc ctacaggatt gcatcaagca
    11761 atgaactccg tgttctggtt tgacgtagca ggaatcaatg atattggtaa atttttggct
    11821 tctgaaggaa caaaaggcgt aacaggaatg tatcaagctg gatttttccc aatcatgatg
    11881 ttcgggcttc cagcaggagc atatgctatt tacagaaatg cacgtccaga aaacaaagca
    11941 aaaacagctt ccttgatgtt agcagcagct tttgcttcat tctttactgg tgtaacagaa
    12001 ccacttgaat tctcatttat gttcgttgct tggccattat atgtcttgca tgcaattttc
    12061 actgggattt ctttagcagt gagtgcattc ttccattgga cagcaggatt tgcctttagt
    12121 gccggatttg tcgattactt cctatctttg aaaaatccga ttgcgaatca gccattgatg
    12181 ttgatcgtcc aaggattagt aacagcagtg atttactact ttggctttaa ctttgctatc
    12241 aaaaaattcc atctgatgac tcctggccgc gaggaagcag atctttcaga tgacgatact
    12301 gcaacgacta attcagacaa taaatatgcg gcacaagcaa gcaaaatcta cgctgcatta
    12361 ggcggagccg acaatgtaac ttcgatcgat aactgtacta cacgccttcg tttgcaagtc
    12421 aaagatacag gcctgatcga tcaaaataaa atcaaagcca ctggcgtacc tggtatgaag
    12481 atcatcgacg gaaaaaatgc gcaagtcatc gtaggtacag aagtacaatt cgttgcagat
    12541 gagatggcaa aactacatgg tggtgctgca acacgacctg cacaaacaaa caccgtggtt
    12601 aaaacagaaa catttgccgg tggaacgatc gaacaagata tttacgctat cgcaaatggg
    12661 aaactgattc caatcactga agtttcagat gatgtattcg cggaaaaaat gatgggtgac
    12721 ggctatgctg tattaccaga aaatggagaa atcttctctc caatcgctgg tacgatcaca
    12781 aacattttcc caacgaaaca tgctgtcgga atccaaacag atgctggtat cgaagtcttg
    12841 cttcatatgg gcatcaacac tgttgactta aaaggcgaac cattcacttt atatgtaaaa
    12901 gaaggtcaaa aagtcgcaag aggtcaattg attgctttag ttgatcttgc cgctattcaa
    12961 tctgcaggca aaaacacgga tatggtcgtt gtattcacaa atggcgacaa agtccaaagc
    13021 ttagaaatcg aacccgcacg tgatgtaaaa gcaaatgata aaatcggctc tgtttccaac
    13081 aaagcctgag ccatcgtctt ccccagactt ttttatcgat aaacaaacag gagctgagac
    13141 aaaaatcttg tctcagctcc tttttccgaa taaaaacggc gttctcctgc caactccgcg
    13201 gtaataagtc cagccaaacg taacatatga agagctattt tttatttgac tcttcttatt
    13261 acttgatgca gaacagcttt tttacaacct ctgataaacg gtgttcaaaa accagtttat
    13321 cggtatgaag tcaaatttat gcaaaaatgt ggaaagccat tttcacaaaa tttttcttca
    13381 tactcaaaac taatcggctt tttcacaacc tctttataca ttattcctga tccaatattt
    13441 cttttaatgc tgcttcaaga gaagttaatg gttttcccaa tactttttct aaatcatcag
    13501 aggaaatagc taactgatcc tttttaatat catgttggat cgctaaaaac atgtcagctg
    13561 cttcttgagg gaatccggct tcttttagat tttcgacaaa cgctgcatca cttgcttcaa
    13621 taatttctaa ctttttacct gtcgcacgct ctaatgcctt ggctaattct tcatagtcag
    13681 ttagattgcc ggataattct aagatttctg gagagtcact ttctactagt acttttgctg
    13741 cagcttctgc atattcacgt ttcaatgccc aacctgtttt tccatttcct gcagcataaa
    13801 cgaattgacc agtttttaaa gcctgcccaa tgataggtaa ttcattctct acataccaat
    13861 tattccgtaa aaatgtatgg gaaatacctg aatccttaat cattttttca gtgaattggt
    13921 gatctggcgc taatacactg atcgagtgat cggcatctgc aaaacttgta taagcgatat
    13981 aagatacccc agctgcttgt gctgccttca ctacattctt atgttcggct tgacgatttc
    14041 ctggaacgcc agagacaaaa agcaatcgat ctattccttt caacgcttct ttcattgagt
    14101 ctaaatcatc ataatcacca atacgtatat taaatccagc ttctttcagt gcttctcctt
    14161 tttcttcact acgtactagt acatagattt cattttgtgg aaccatcttt tttaagaatt
    14221 ccagcgcata accaccaaaa cctccagttg ctcctgttac taaataatgc atatttgcca
    14281 ctccctattc tttttatttg ttaagattta cagctgtaca tttataaatt acacctttat
    14341 cataaagagt gtggtaagat aagtcaagca aaaaaaatga aaggatgaca actgatgaag
    14401 tattctctgc aatttagtga tgcaatccat gtacttgcct atattgaaat gtttcaagat
    14461 acccgatcac tttctagtga gatgattgcc agtagtatcg aaacaaatgc agcaaatgta
    14521 cgtaaaatca tgagtcagct caaaaaatcc ggtttgatct ttacaaaaac cggaaaaccg
    14581 cagccttccc ttgccaaaag tccagatcag atttctttac tagatgtcta tcgcagcatc
    14641 gaaggtaata cacagcttat ccaagtcgat ccaaaaacaa atccaaattg cgtcgttggt
    14701 gcgaatatcc aagaagcttt agaagaaagc tacgctcggc tgcagaaaaa ggcagaagaa
    14761 gaaatggcta agatcacact tgatagtctg atacataaaa tagccgtttt ggaaaaagaa
    14821 aaaagaccag aaaacacagg cctcgtagag agatttttat aattctctct actgtgcctg
    14881 catttttctg atcggaatcg tctacataaa ttcttttatt ctccggggga caaaagcctt
    14941 gtcatgcccc tttttcctct ctaatgctca ggtcaaagcg ctgaagtcat gacgtcttga
    15001 taaacggtgt tcaagtagct gaccctcggc attagttctg aaaaatgcaa aatatggaaa
    15061 gctatttccc atttttcctc tctaatgctc aggtcaaagc gctactttca cgacctctat
    15121 attatcatgg tttattgact accggtatcc agatttctcc catatatttc ccgttggtaa
    15181 ctcccatttc gactacagca tttggacccc caacataggc gtaatcttct acttctctta
    15241 aaacatgccc aaaggccgta ttcgtcaaat catttcttaa ttcttcaggc gattctgcta
    15301 aagcgattac aacaagatac gttccttttg ggaatgggat gattcgggta gtgtttggaa
    15361 catcctgact cgtttgaaca ccgacatagt acatcatttt cccgttatag ggttcgttga
    15421 ctgtaaagaa ataatgattt tcagcagctt gtttcaatgc atctattgtt ccattaccga
    15481 taactttttg ctcaaatgct tgtttctctg cagccaaacc gacaaaatcc gtattgctac
    15541 tcttcaattc cacaccaata cccgtcaccg taaaatctgg tttttcttcg atcctatact
    15601 ttgccatttc tcccaccctt tctattatat taagttcatc ttacataaaa ttaaagggaa
    15661 atgacacgat tacgctcttt tttctaggga tatttttatt ttttaactat ttttttgctt
    15721 tcactgcaat ccaaatctca ctgtaaacat tactgaaatc ctctccccat tttgtaaaag
    15781 agatctcggg agcttcgact aactcataat ctgaagaagg aagccattct gcatagattt
    15841 tcccccaagt atcttgtaaa gtttggggaa atggcccttt attaggaaaa atggcccaag
    15901 tgtgttcttc tatagcaagc atttctaaat cagcgtacgg attttctttt gtcgtagcaa
    15961 agccaatcat gtgcatcaaa ctgccctttt cttccaagcg ggctccatca aatccataag
    16021 aaacgttgat tacttgattt ggatataaat ctcctaactt atgcatctct tccctttgag
    16081 tctccgtgat cgtctgcgct aacttttgta tttcttgatt gactccttca aatttgacag
    16141 gaactttttt cgccacacct gccacataaa actgttcttt cttttcgata cgaaattcca
    16201 tcgattttcc tcctctgaca tcaataaaaa atgtcaagcg tggaaaagat ttttgtatgc
    16261 cgtatctgct gatttctgaa ggcagatatc ctgaccaatc acgaaaagct cttgaaaaac
    16321 cttcgactgt ttcatatcca tatctaaacg ccgtttctgt cactcttgat ccatctagga
    16381 gatcgcgatt agccaaagcc aatctacggt ttttgatata gtccattagc gacatccctg
    16441 caataaacgt aaagatgcgt tttaattgat attcagagat tcctgtattt tcggctatta
    16501 ttttatagga aagtccttct gtcaaatgtt cttcgatgta atcgatcaat tggttcagat
    16561 ctttaagcat atttatcttc ctccttgctt gcctttttat catattatca aagaaagtca
    16621 attacttgat gaatcaggaa ccaatttagt cagatgtaga aaatgttgta tggatctaaa
    16681 gaaaattctc aagcagcgtt tcgtaacgga taattggtat tatctgccta tctatgtttt
    16741 acctttagaa aaataaattt tggggaagac acattctaag taaacaaagc ggtaaaaata
    16801 tcttttattc ccttagttaa tccgcttact acctactgta caaagcaaat tgttgttcaa
    16861 actgctgatc agaacgctgc ttggattttt ctttgcgata catcatacct cttttatcaa
    16921 ttggaacaat tatttatttc taacaaaatc acttcatttt tgtcagataa ataaaaaaca
    16981 aaaaaagttc catctttaaa acacgctatt cttttacttt ttctcacatt ctctaagaaa
    17041 agaaaaggag aatgaatttt tgacaattcg agcaaaagtg attatcaaat atgtaaaatc
    17101 agctactttt ttcttaaacg tttctttata ctaaatataa ataagtgttt catgaaagga
    17161 gaactagatg aataattggc ttgagtattt tcctgaaaac gttttggaac gcggttacag
    17221 ctatcatctg cacggttttg tccgtcattt gaactataca agcaagtact tgtccgcaac
    17281 agtttctggc acggaagatt ataaggtagt gatcacttgg gatgaaaaga ccaacatgac
    17341 ttgtgattgt ctatatgcca tagaaggaaa aaaatgcaaa cacatggctg ctgtcttatt
    17401 cgcttacgaa gaacgtccaa taaaaaaatc caactactca ctttccgaac tctcgtccct
    17461 tgtttcctcc gctagttcct ctcttgtacg ggagctttta acagagattc ttatagaaca
    17521 tccgcaattt atcgaacgct tcaaagttaa gatgccattt catgcaataa attattcaga
    17581 taaattaaca actatcatcc ataaatacga tcatattatc aagaaaaaca aaaacaggaa
    17641 aacagcgaag ttcatcatgg agatgcgaaa gttcatccaa gaagcagttg aatcgctcat
    17701 tcagcaaaat gcctatcttc ccgcattcga attgatcaat gaagtgattg ctactctcga
    17761 aacattctat tgggagcctg aggatgaacg aacccttctt ctgatagagg actgttatta
    17821 tttatggaaa gaattattag cagaagcacc tcatgcagaa aaaaggcaaa tgttctcttg
    17881 gttcgtctgc caagtcgatc atactgacgc ctcttattca aaacgctact ccataaaaat
    17941 cctgaaagaa gactttcggg aaaaagaatt ttctaaccaa aagaaaaaaa tagataaaaa
    18001 aactaaagaa acggtaaaga aagacgattt caatgaaaaa tagcaagtaa acgataacaa
    18061 ccatcctaaa tccacaagaa atacagcaga ccttgcttta aaaacacgaa tcatgcaaag
    18121 gatgaagtaa agaagactta acgcgtaccc aatattagtc atttgactat taggtacgcg
    18181 ttttgtttta taagtcatga caaatttcct tcgttgctgg gtagattttc cggcattgtt
    18241 gatagatttt atgatacttt tctacatttt gtaagagagg ttccactgca tctgtatatt
    18301 ggataaaatt ctgacaacaa ctttcaggtg tgtcgaacca ctctaatctt taaagataaa
    18361 tattaaaata acgattaata atattaacag tctttatttt tcaccaccaa aaacctatct
    18421 ttgcatatac taaatagacg tatatacaca gataggtttg tcttgtgaaa aataatgatt
    18481 atccttacca aaaaatgttt gtttttctac atgttcgtac ttatcaaaaa atatgatcct
    18541 tattgaaatt actggctatt tatattccaa cgacttcttg tgttaatttt tgttatctca
    18601 tagtaaatta tgtttcattt tcacttaatt tagttgtcac gtgttcgtca attcgtaaaa
    18661 acggtaaata tataaacaca cttgcggcta taattaatat ttgtaaaaca gctgctcgcc
    18721 agtctccggc tgttgctaaa aatcccgata taactggtgg cgtagtccaa ggaatttgca
    18781 ctactacatg attaattaat cccacagcag ttgcaaaata tgctactgtc aaagaaaaaa
    18841 tcggtgcaag cacaaaggga ataatcagac tgatattaaa tacaataggc aatccaaaaa
    18901 taataggttc gcttatattg aatatagcgg gagttaccga taatttagca aagtccttat
    18961 aatcttttct acgactgaag ataaatattg caatcaataa agcaatcgta tttcccgatc
    19021 cacccattac cgcaaaggta tctttaaagg ccatattgat aatatgtgga atttgctctt
    19081 tgtttgcata agctgccata ttttcttgca tattctgaat caaaaacgga tctaacaatg
    19141 aacctgatat tactgcttga tggataccaa atccaaaaaa tagattagca atagatgtaa
    19201 ttaacaaaaa tcctggtaaa ccagttgtaa ttttacttaa tggctctgta actatcgtgg
    19261 taattagctt atttatatcc atattaaaca attgaatgat tataaatgac aataaagcaa
    19321 acgaggtaat cgtaataatg attgggatta atacattaaa agactttaaa acagcgggag
    19381 gaatatttcc ggatagctga atctgtagtt tcttattatt agaaagttta ataaataatt
    19441 ctgtagccgc taatccaaca aatataccta caaacattcc tgaagctcct gtataggata
    19501 taggtatcac acccgtcact tcaacagctt tatccgcatt ttcaggagta aacatcatct
    19561 gcataggtgt taatacaaca aatgtcgaaa tagcaactaa taccgctgca atatgatttg
    19621 tataatttct gcttgcacac atatgataag atactgctgc tacaactaat aatgtagtta
    19681 tgcttaatgt accattggca attgaagtac caatttgttg taacgtggtc aatgtactag
    19741 tatcgacaat ttcccccatg aatcctgttg gttctaagat cactgaatta ataagtgtca
    19801 caaatcctgc taacatcata aatggcataa atgtagcaaa cgcatctcgc aaagttctta
    19861 aatggatctg attccccaac ttagtcgcaa ctataccaaa tttttcaaat acaatgtcct
    19921 tcctgctact tttgcccata ttttttctcc tttatgctta ttgtaatcga ttactttttc
    19981 cacccaaata atacatcact tatcaaaaaa agtaaatact tttaattaaa tatatatatc
    20041 ttttttagta taaaaaaata agctctgaaa aaataatttt cccgagctta tttttcaaca
    20101 aaaacagctt caaaaacaaa gtcttgatat aaatgtctcg ataccgaata ctcgaaaggt
    20161 ttaccagacg atagagcaac aactttgtct atctcaatca taaattcacc tggatctatt
    20221 tttaatattt ttgcgtcaaa ttcattcgca gtatctcctt tgatacgtat gtgcgaagtc
    20281 tttatttcca gacccagttc atttgtgata taagcatata cagatttctt taaatgtttt
    20341 ggttccaagc ctggtatgac tgctaatggc atgaatgttt gctcaatcga gtatggttga
    20401 caatcaataa aacggcttcg tacaatatta tatacatagt cttttacact gatacttaat
    20461 ttatcagcta cttttttcga tggaagttca attgagaaag tatggatttc tgaatctacg
    20521 tgttcttcac caaccacgct aaacaaacct tcgtttggac caagaactcg tgagttatca
    20581 attggtgttc tgacgacttc agtcccatac ccacttctac ttcgcaaaac tccttcggaa
    20641 acaagtaagt ctacgccttt ctttactgtt aacttgcttg tatgataaat aactgccaat
    20701 tcatctcctg taggaagttt ctcaccaatt ttatactctc catttacaat ttttttcttt
    20761 atatctaagt aaatttccat atacttcgca atttttttca atggtgaccc tccaatcaaa
    20821 aacatatctt catctatact ttaacataaa taaaccgata aaatccacat caaaattata
    20881 cacacaataa aaaagatata tatatttaaa caaaactatt tactttaagt tgtaagcggt
    20941 gttatcatta agtggaaaca ggaggtaaaa aaattatgac aactatgaaa aagctaactt
    21001 ttccagatga tttttggtgg ggttccgctt ggtcagctga gcaagcagaa ggaaaaggag
    21061 aaacaggaaa agcagagact atctgggatc gctggtttaa agaacaacct taccggtttt
    21121 atcaacgaat cgattcctct attacaaccg atcacatcca tcaatataaa gaagatatag
    21181 aactaatgaa aaaaactggg cataactcct ttcgcgtttc tatttcttgg gcacgaatgt
    21241 ttccaaatgg cggtaccgga aaagtaaatc ccaaagccat ttctttctat cgaaaattat
    21301 tttccgaaat gaataataac ggaatcaaag tatttgccaa cttatatcat tttgacatgc
    21361 ctgctactct tcaagataaa ggaggatggg aatcacgtga agtagttgat gcttatgttc
    21421 attacgctga aacttgtttt aaagaatttg gtgacttagt ttgtcattgg tttaccttca
    21481 atgaaccact tgggcctatt ttaggtactt atcttgaaga ttttcattat ccaaacctca
    21541 ttgactttaa acgcggagcg caagcagcat tcttcactat acttgcacac gcaaaagcaa
    21601 tacaagtatt taaagaacta gctcttccta gtaaaatagg cgttattcta aatttgagtc
    21661 ctacttttcc tcgtagtcaa aatcctgctg atgtgaaagc agcagaaatc gctgatttat
    21721 tttatacgcg gagcttttta gatcctatgg ttaaagggaa tttttctaaa aagcttatcc
    21781 tgttattaca agaatataat cagcttcctg aagattatac gaaagaagat cttgtatgta
    21841 tctacgaaaa taccgctcaa attcttggac taaactatta cgagccacgt cgcgtcaaat
    21901 ctcgttcttc tatggtggat acatctagtc catttttacc agagtggttt ttcgaaccac
    21961 atatcatgcc tggacgacga atgaacgaat accggggatg ggaaatttac gaaaaaggaa
    22021 tttatgattt gtgcatggat atcaagaacc attatggaaa catcgaagca tttatctcgg
    22081 agaacggaat gggcgttgcg gatgaagaac gtttcatgga tgatacaggg caaataattg
    22141 atgattatcg gattgagtat atcaaagatc atcttgctta cttatggaag gcaataaatg
    22201 aaggatgtaa cattaagggg tatcatcttt ggacatttat tgattgctgg tcatggatca
    22261 acgcctataa aaaccgatat gggctggttt cactcgacct tactactcaa aaaagaacaa
    22321 ttaaaaaaag tggcgaattt tacaagcaat taagtgatac aaatggattt agatacgatg
    22381 tagacttgtt agttgaataa tattctaaaa ttccacttga agtgaacaca ttagaaaaca
    22441 agaataatga gaaataccaa taacaaatac tttttcttca tacttcattt atacttgtca
    22501 gtataagaga ctgtgacaaa tgtagtgatc ctcagaagtt aggtctggaa atctaacttg
    22561 agaagatttt tttaaactac gtccagtctt ttttatttgt ttaaaattga agttgacaag
    22621 tcttcattaa aaatttagaa tattcacaag tttgacgaca attatgaaaa tttatgtcgt
    22681 gctaaaggag ttttgttatt ggatgaaaag ttagatataa aaactgcgat caaagacacg
    22741 ttacctaccg tttttggtta tatcggtatt ggacttgcat ttggtatcgt tgggaaagct
    22801 gccgggtttc atccgctggt cgtcacgttg atgtccctgc tgatctatgc tggttctgcc
    22861 caatttatca cggtcagcat gcttgctagc catagtccac tgctctccat cgttctctcg
    22921 acctttctag tcaattcccg aatgattctg atgagtatga cgattgctcc ttatttcaag
    22981 aaaaatcggc tgcttcaaaa tctgctgatt ggcacattgc tgacagatga aagtttcgca
    23041 ctgggaatga ataaattgaa ctatactgga caaaagttaa actttcgttg gatgaataca
    23101 gctaattgga tttcttattt gacgtgggtg ggttcttctc ttgttggcgc acttttaggg
    23161 aattttatta ctgatccgaa aaagtttggt ttggatttcg ccatcgtcgc tatgtttatc
    23221 gggttacttt atcttcaagt catttctgat agaaacacct ctaaacgatt acagttgatc
    23281 ctgattggtc tcactttgat tctggtatat gtcggactga tttttatccc aagtaactta
    23341 gtgattttag ttgtaacact gattgggtgc ggattggggg tgtggatcaa acatgccttc
    23401 ttttgagttt acattgctga ctattttagg ttgtacttta gcaacttggc tttctaggat
    23461 cctaccattt gtcttactga aaaaattcga tttgccgcaa ccgttgcttg agtatttaag
    23521 ttttgtcccg atcgtcatta tgtctgcttt atggttcagc agtctattta cccaaaatat
    23581 cggacatctg ccgcagatca atctcgaaaa tgctttggct tcagttccta cacttttagc
    23641 tgctattctt tcaaaaagct tattggtgat cgtcttggta ggaattcttt ccctttcgtt
    23701 gattcgctta ttataaataa aaaaacaaga gcttatggca aattactgtc atgcgctctt
    23761 gtttttatca aagtcaaata agaaaaaaat cggcgtattc ttgcttatcc gttgaacgga
    23821 ccactaacaa cgacttttcc taatatatgt cctgtttcta cgtctttggt tgcttgtttc
    23881 aagttcttcg cattcatccc atctgaatag accttgctca atgtactgcg gataatccct
    23941 tcatttgcta aagcagcaat gtgcgctaaa gcttctcctt gcgtttcgat ttcatagtta
    24001 tagtctgttt tagcgaacat atactcccag tcgaagctga tagattggtt tttccaaaca
    24061 cctatatcta acggctcttt gatccctacg atcgttccca catgtccaaa tggtcggaca
    24121 aggtgtttga tttgatcaaa gtagcgggta atatcgaata atacagcgac atactctact
    24181 tgatcgattc ctaattcacg aaaactttta cttaagtcgt gatgataatc gattggatag
    24241 tctacacctg tttttttcag ccattcaaaa ttcttcggac ttgcagtcgc gtagacttct
    24301 aggccagccc aatgcgctag ttgattcaaa atcgaaccga cgcccccagc tccgttgatc
    24361 actaagattt ttttcccgcg gttggcattt tcttctggag tcagaccaaa tttttcaaat
    24421 aacaattcat aagcggtcaa tgaggtcaac ggcaaggctg cggcttcttc atctgaaaga
    24481 ttttttggtg caagagcgac aatacgttcg tcaaccagtt ggtattcttg attgcttcct
    24541 gctcttgtcg ttgtacctgc gtaaaaaacg cgatcgccga cagaaaattt ttgcacttca
    24601 tcacctacag caacaacttt cccgactcca tcaaacccta agatacgtaa agcatttctt
    24661 atcccgttat cttggcgaag tttcgtatcg acaggattaa cagatactgc gttgactttt
    24721 accagaagat cccgtgaact gggtacaggt atctgttctt tttcatcaat aaagcttttt
    24781 ggatcatcga tcgacaaccc ttcataaaaa ccgactacct tcattaattc cttttctgac
    24841 atacgaattc ctccttagct tcatcaagtt ggagcatcca tacagttttc atttcaatat
    24901 atatagtatg ctatttgttc cacttttatt catcataatt attctcttac ttaaagtaaa
    24961 ctatttgcac ttttttggtt aaaaaagagg ttgcgacaag acttttgtca caacctcttt
    25021 taaacggtgt tcaatcaact gaccctcggt attcgctcaa aaaacgacaa aatatgagga
    25081 gctatttttg tttggctgtt cttattactt gatgcaggac agctttttca cgacctctaa
    25141 ttcacggtgt tcaagcaaga aatcattgtt cactgaaaag cgttggatgt tctttttcaa
    25201 aggtgtctaa gatctctcgt acttcatcag tagatttcgt attcatcaac tgaactcgca
    25261 agtcatttgc tcctggaaat cctttgatat agattttaaa gaaacgatgt agtcctgtga
    25321 tggaacgcgg gatgatttct gaataatgat cttgcagatc tagttgcaga cgaagtagtc
    25381 cgatcagttc tttttctgta tgttccctcg gttctttttc aaatgcataa ggattcttga
    25441 aaatcccccg gccaatcatg atgccatcta cgccatattt ttcggccaat tcttctcctg
    25501 tctggcgatc tgggatatct ccgttgatcg tgatcagtgt ttgcggagcg atttggtcac
    25561 gtaaagcaat gattttaggg atgacttccc aatgtgcatc tactttactc atctctttac
    25621 gcgtacgcaa atggacagaa agattggcta tatcttgatg aagtaggtga ctgatccatt
    25681 cttccatctc agacatttcc gtatagccga tacgcgtctt gacgctgaca ggcaaaccgc
    25741 cagctttagc cgcttcaatc aattgtgccg ctacttctgg gcgaaggatc aagccactgc
    25801 cttttccacg ttctgctaca ttagggactg ggcagcccat gttgatatcg attccttgga
    25861 agcccatttc tgccatagca atgctcattt ctctaaaaaa ttctggcttg tctccccaaa
    25921 tatgggcaac catcggctgt tcatcttctg taaaggtcaa tcttcctcgt acactgtctt
    25981 ttccatctgg atgacagaaa ctgtctgagt ttgtaaactc agtaaagaag acatccggtg
    26041 ctgctgcatg ctttacgaca tggcgaaata caacatctgt tacatcttcc attggtgcta
    26101 aaacaaaaaa aggctttggc agctctttcc aaaaattagt agtcatttat ttgttactct
    26161 ccattctatc aagcagtgaa gtcgcaatct ttctcaaaaa cattgtcatt atactaggaa
    26221 acaaaaaaag aagcaaagtg aacagcttaa aagatgtttc tgattgagca aaaaaagact
    26281 gtgacaaaaa tcttatcaca attccttttc cggaataaat ggtgttcaaa agcttgcatc
    26341 tgcggtaata ag
//
LOCUS       DACDYI010000045        25743 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.045, whole genome shotgun sequence.
ACCESSION   DACDYI010000045 DACDYI010000000
VERSION     DACDYI010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.045"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..769)
                     /locus_tag="IWM52_001726"
     CDS             complement(<1..769)
                     /locus_tag="IWM52_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HAP9973975.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNAL"
     gene            complement(974..1324)
                     /locus_tag="IWM52_001727"
     CDS             complement(974..1324)
                     /locus_tag="IWM52_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9973976.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            1919..2365
                     /locus_tag="IWM52_001728"
     CDS             1919..2365
                     /locus_tag="IWM52_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP9973977.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            2989..3981
                     /locus_tag="IWM52_001729"
     CDS             2989..3981
                     /locus_tag="IWM52_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9973978.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            3997..4881
                     /locus_tag="IWM52_001730"
     CDS             3997..4881
                     /locus_tag="IWM52_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9973979.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            4930..6537
                     /locus_tag="IWM52_001731"
     CDS             4930..6537
                     /locus_tag="IWM52_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9973980.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            6538..8022
                     /locus_tag="IWM52_001732"
     CDS             6538..8022
                     /locus_tag="IWM52_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAP9973981.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            8199..9191
                     /locus_tag="IWM52_001733"
     CDS             8199..9191
                     /locus_tag="IWM52_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9973982.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETIEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            9243..10112
                     /locus_tag="IWM52_001734"
     CDS             9243..10112
                     /locus_tag="IWM52_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9973983.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(10532..12097)
                     /gene="guaA"
                     /locus_tag="IWM52_001735"
     CDS             complement(10532..12097)
                     /gene="guaA"
                     /locus_tag="IWM52_001735"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP9973984.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(12156..13079)
                     /locus_tag="IWM52_001736"
     CDS             complement(12156..13079)
                     /locus_tag="IWM52_001736"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP9973985.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            13275..13880
                     /locus_tag="IWM52_001737"
     CDS             13275..13880
                     /locus_tag="IWM52_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9973986.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            14188..15204
                     /locus_tag="IWM52_001738"
     CDS             14188..15204
                     /locus_tag="IWM52_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9973987.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            15345..16646
                     /locus_tag="IWM52_001739"
     CDS             15345..16646
                     /locus_tag="IWM52_001739"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAP9973988.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            16692..17354
                     /gene="deoC"
                     /locus_tag="IWM52_001740"
     CDS             16692..17354
                     /gene="deoC"
                     /locus_tag="IWM52_001740"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP9973989.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            17375..17773
                     /locus_tag="IWM52_001741"
     CDS             17375..17773
                     /locus_tag="IWM52_001741"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP9973990.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            17839..18921
                     /locus_tag="IWM52_001742"
     CDS             17839..18921
                     /locus_tag="IWM52_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9973991.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            19133..20695
                     /locus_tag="IWM52_001743"
     CDS             19133..20695
                     /locus_tag="IWM52_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9973992.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            20688..21821
                     /locus_tag="IWM52_001744"
     CDS             20688..21821
                     /locus_tag="IWM52_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9973993.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            21821..22777
                     /locus_tag="IWM52_001745"
     CDS             21821..22777
                     /locus_tag="IWM52_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9973994.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            22876..24042
                     /gene="deoB"
                     /locus_tag="IWM52_001746"
     CDS             22876..24042
                     /gene="deoB"
                     /locus_tag="IWM52_001746"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP9973995.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            24066..24884
                     /locus_tag="IWM52_001747"
     CDS             24066..24884
                     /locus_tag="IWM52_001747"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP9973996.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            24901..25608
                     /gene="deoD"
                     /locus_tag="IWM52_001748"
     CDS             24901..25608
                     /gene="deoD"
                     /locus_tag="IWM52_001748"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP9973997.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT         8096 a         4470 c         5664 g         7513 t
ORIGIN      
        1 ctaaagcatt gtaaaaaaat ggacgtctaa ctatatattc gcttgtatat agtatatctt
       61 gattcgtgga atcatagggt ttctttacca tctttttggt gacagttggt agtttgagat
      121 tccgggactt gaaaaaagct tttttcttat gctctttcaa ttccagctct ttatcgaaat
      181 atttgctgat atagcgcccg cgattttcga tactgtctac atcgatcttg ttgatcttaa
      241 cgaaaccctg accccaaatc gtagttaacc ggttaaatga gataaagggg aaatcaaaca
      301 agataatatg gtagtggatt gcaccgcgtt gctgcttttc ccacgtggcg agatacttta
      361 aatttgcttt tttagtttta tagaggtcat aattcagtcg tttgataaat tttttaaatt
      421 cattatttgc atactcaatg tcttgaatgt tttctttgaa agtgagagtt aaaaatttcg
      481 tttgattgtc aaaattcgtg tctacaaggc gggcaatctc aaatcgcata ctttgataat
      541 gtttctgttt gcgttttaaa ctgtcatatt gtccttgagc ggataattca tcatagtttt
      601 ttcttttttc aagagtaata tcattttcat aaatccaagc taatttttgt tttcgttcca
      661 gatttacttc gttttcgatc gttttacgtt tgggagtaat gattgcttca taatcataaa
      721 tttcaatata cgtgggagtt tcaatgattt tctgattata agcttgcaaa aatcatcact
      781 ccttgttgcg tattcgattg tcaaagaacc agtcattttc tcctgatgtt gatctataaa
      841 tcaagttaag ggggaaggcc tgcgtagcag cccttctagc ctatggggtg ttgggcggtc
      901 ttaacggaca gaaacggctc tgtgggcgct gccccgccgt ttcgtcctaa ccacccaaca
      961 ctccatagta tagttagttt tgtgtagtgg taccttgttt tctgggaatc aaagttgccg
     1021 tatcagcggt tgccgataac ccataactga caaattgtcc attcgagcgt ccccaaggta
     1081 caatcaccaa attggtaaat gtaattatgg ctaaaccttg ttgggtaagt tcttccggcg
     1141 tgatcacagg atgttcagat ttgactttga cactgatttt ttcgtagcga agttttggta
     1201 ggatacattc gtagctccaa cctagaattt cgccctcctt ttgccagcga gtgacattga
     1261 cgacttggta ttctttttcg agtgtttctt ttggtaaact gagatcggta atttttattg
     1321 gcatcacaat tctccttttt tatacgaaag tttatacgat ttgattaaaa aatatagatg
     1381 gccatctacg acttgtatta tatacgagaa cttatacgta gtcaagagat tttctaaaaa
     1441 aatatatacg attttttata cgagtgttat aatgaaaaaa atgataagga ggaatttgaa
     1501 tatgtttggt ttagtacaac ctaataaagt gaaggtgggc caacgaatca aagaaataaa
     1561 agacggaatg tctctctctt ttacagaatt agggaatcga ttaggtttaa aaaagccgac
     1621 gatcagttcc tacgttcaag gctatgcgtt ggctcctaag agtgtgatca accaattatc
     1681 tagtatcagc ggaaaacctg tcggctggtt ttattttggg catgtggaag aatatatccg
     1741 agagtatttg tttctcattg gtcagaaaaa aatagtggac gattatcctg aaatcattga
     1801 gaaaataaag caagaatttt atacgggcga gttcaaaaat cctgcttggg agaatgagct
     1861 aggctatcct ttggaggaat ttatccaaga atgttttgta gaatattcga gtgaaatcat
     1921 gaaggactat gtcgtacaga ttacgaaaga ggtacttgaa gaatccaaaa cgctaaacaa
     1981 actatcatca aaagcaaaag aagaagctgt cacgattctt tcatctggga tcattgacta
     2041 tatcgagatg agtggtgaga tcaagtatgg agagaaagat cgcattgcga aaatggtgaa
     2101 agaatcaata aaaacctttg atgtgaaaca agacctgaca tttaatgatt cctatttgat
     2161 tggaaagttg atcaatattc ttggtacaga tgaagatacg gagctgtttc tttcttcttt
     2221 atcaatgata tttactgata agcatttttc cactcgattt ggtggggaag aattagtcga
     2281 aatatttcaa tcgatgcgac cagcgttaat caaactctat cgggagaaaa caccagatga
     2341 attttatgat tggtttgaga agtaaaataa gaaaagtggc ttagattaca agttggtgaa
     2401 aaaatttttt tcagcataac atcagtaatc tataaagggg ttgttgatta tactgtagaa
     2461 gttaagtact caagagattt tttgtacgta aacaaagcaa aaaagggtgc agtttcacta
     2521 gaaaatgtac tcttttttgt gatttttctt agttatagag tttttttgaa actttaaaaa
     2581 tgagaaaaca tattgatttt aaagagtgta gacatagctt cttttgctaa atagattaaa
     2641 cttactggtg agtggtcttg aacatacttt taaatcacat tttactgagt tttattggat
     2701 tttataattc attggaaacc tcgtacttaa tctgttttta tctatcgact agaatctgta
     2761 tatattccag ctatcaatga ggccgcagaa aagggtgctt aaaaataatc tgttgtttaa
     2821 aatattatta tcagaatact tagatacaaa attctggcac aaagtaaaaa aaatattgac
     2881 ttaaaaaaat ggatatggta atctataaac gaaaatagtg gtaacgtttc caaaatgcat
     2941 cttcgttaaa tttttatttt tttaaacgaa aatggaaacg tttctaaaat gtattgggag
     3001 gagattgaat tggaaaagac attgggacca ggtggtgaga taagggcggt acaacgggat
     3061 aaaaaaaata tttcgaagtg ggaaaaatta aagaaacagt tgcggcgtga taagtggatg
     3121 tacttgttgc tcttaccagg tatcatcatg acgtttatct ttaagtatgt gccaatgtac
     3181 ggtgctgtga ttgcatttaa agactataat cctatgctag gaattttgga aagtccttgg
     3241 gtaggactag atcactttag ggaatttatc agttcaccaa attttacgct tttgctgagc
     3301 aacacattaa aaattagtat ttatggttta ttgttaggtt tttttccacc aattatttta
     3361 gcactgagct tgaatcaaat tatcaataaa aaaataaaac agaaaatcca gttgattcta
     3421 tatgcgccga attttatctc agtagttatt attgtgggga tgctgttttt gttcctatcc
     3481 gcaaatggac ctattaatgg gttagtagaa aaggttacgg ggcaaccaat tatgttcatg
     3541 tctaatccag cttatttcag aacgatttat attttatcgg gaatttggca aggaatcggc
     3601 tggtcatcca ttctttacac cgcaacgctg gcgggagtca gtaccgattt atatgaggca
     3661 gcaaatatcg atggggcaaa tattttacaa aagatttggc atatcgattt gcctgcattg
     3721 aggcctgtga tggtgatcag cttcatcttg gccgccggcg gtattatgaa tgtaggatat
     3781 gaaaaagcct acttgatgca aacttcaatg aacatccctg cctcagaaat catttccact
     3841 tatgtataca aagtcggctt gcaatcaggt gattatgcct actctgctgc tgttggtctg
     3901 ttcaatatga ttattaatgt cgttttatta ctgattgtca acaatataac caagcggcta
     3961 aacgatggtc aaggcttgta atgaaaggag aaaattatga atagtgcttt atcaaaaatc
     4021 gaccgtcaaa tcttatggtt gaatcgagtg attgtaactt ttttagtcgt catcaccatt
     4081 gttccgctga tttatgtact ggtagcttct tttatggacc cagctacttt gatcaataaa
     4141 ggtataagct tcaatccgaa agattggaca atacagggat atcaacgggt atttgcagat
     4201 gattccattt tacgcggttt tgtaaattcg ttgttttact cttttagctt cagcttattg
     4261 actgtattta ttacgatgat cactgcgtat cctttgtctc gacctaactt ggcggggaaa
     4321 aagattatta tgactttttt tgtcattaca atgttcgtgg gtggtggctt gatacctacc
     4381 tatttattgg taaagaactt gggcatgtta gatagtgtat gggcgattat cgtaccaggt
     4441 gctataaatg tgtggaatat tatcttagcc cgtacctatt ttagtgcttt gccaaaagaa
     4501 ctttctgaag ctgctactat cgatggcgcc aatgacttgc aaatactgtt caaagtcatg
     4561 cttccattag cgaaaccaat tatgttcgta ttgtttttat atgcctttgt tgggcaatgg
     4621 aactcatact ttgatgccat gatttacatc aaagatcccg acttacagcc gttgcaactc
     4681 gtattaagaa atattttgat ccaaaaccaa ccttcacaag acatggtggg agccaatact
     4741 gccatggcag aaatgaaaca aattgctgag ttgatcaaat acgcaacgat cgtcatttct
     4801 agtctgccat taatcataat gtatcctttc ttccaaaaat attttgataa aggtgtttta
     4861 gttggttctg ttaaagggta agtattgttc actatttgta tgaataaaaa ttataaagga
     4921 gattttaaga tgaaagcaaa atggatgctg ccaatcgttt ttacttccgt actaattttg
     4981 ggggcttgtg gaggctcaaa agaaaagagt aaatcaagcc ctgattatga actagaaaat
     5041 gtctcttttc cattgaaaga aaaagtatct ttaaaaatga tgtctcaaag tgcccctttg
     5101 gctcccaatg atcctaatga caaattgatt ttccaacgat tagaagaaga gaccaatctc
     5161 catattgatt ggacaaacta cagtagtgat tttgccgaaa aaagaaattt ggatatcgct
     5221 agtggtgatt taccagatgc catcttcaac gccgctgcag gtgactatga tttattaaac
     5281 tgggcggaaa gtggcgttat tgtaccgatt gaagatttga tcgaagatta tatgccaaat
     5341 ctgaagaaaa tttttgatga aaaccccgaa taccgacaaa tgtctactgc acctgatggt
     5401 cacatttact ctatgccttg gattgaagaa ttaggcgaag gaaaagagtc gattcataca
     5461 gtaaatggga tggcttggat caacaaggag tggttggata atttgggtct tgaaatgccg
     5521 caaaccactg atcagttgat gactgtgttg gaggctttca agacgcaaga tccaaatggt
     5581 aatggtgaag cagatgaaat tccttttagc tttattaaca atggaggaaa cgaagactta
     5641 aaatttttaa tgggggcatt cggtctaggt gataatgatg accacttagt cgttgataac
     5701 gatggtcagg tacaatttac tgctaacaaa gaagggtata aagaagccat caaatatttc
     5761 aatgaaatga ataataaagg tcttattgat aaagaggctt tcgaacaaga ttggaatacg
     5821 tatgttgcca aagggaaaga acataagtat ggcgtgtact ttacttggga taaagcaaat
     5881 atcacgggtg cagatgatag ttatgacgtc ttgccagtct tagcgggacc agatggaacg
     5941 aaacatgtta ctcgtacgaa tggcatgggt ttctcaagag atcgtttcgt aattactagt
     6001 gcgaataaaa acttggaact tacagctagg tggatagata aaatgtatga accgttacag
     6061 tctgtccaaa ataactgggg aacctacggt gatgaagcac aggcgaatat ttttgaatta
     6121 aaggatggta tgttaaaaca tttgccttta gaggggacag caccggggga actaagacaa
     6181 aaaaccgaag ctggaggacc attagcagtt cttgattctt attacggcac agtcactact
     6241 atgccagatg atgcgaagtg gcgtttagat ttgatgcatg acacgtattt gccatatatt
     6301 acagaagaca atatctatcc acgtgtcttt ttagaaaaag aagaaacaga tcgattagca
     6361 aaaatcgaaa cggatatgtg ggattacatc tatcgcaaaa gagcagagtg gatcaccggc
     6421 ggtaaaatcg atgaagaatg ggaagaatac cttgctgaat tggaacgtgt aggtttggaa
     6481 gaatggattc aaataaaaca aactggctat gataattttt tgaagggagc acaataagtg
     6541 ttagtagaag aaacggcgat tttggataaa gcaaatcgat ttattgaaat gatgcgagga
     6601 aaagttaacc ctcagtatcg acaaaactat catttttccg cacccatcgg gtggataaat
     6661 gaccccaatg ggtttatata ttacaaaggg cattaccatc tcttctatca atactatcct
     6721 tacgatagtc gatgggggcc catgcattgg gggcatgcgc gcagtagtga tttggtccat
     6781 tgggaagatc taccagtagc gttagcccca gatcaacctt atgacagcgg cgggtgtttc
     6841 tcaggaagtg caattgaaag agacggaaaa ctatatttat tttatacagg ctgtcaagaa
     6901 attgatggta ttagccatca gcatcaatgt ttggcggtct ctgaagatgg tattcatttt
     6961 agcaaataca gtggtaatcc tgtggtagca gaaaaacagc taggtgaaaa tgggctaata
     7021 caagaatttc gtgatcctaa agtaattgtt cacaacgatg gttactatat ggtagtagct
     7081 actaaatcta cacagtctac tggtagaata gtgatgtttc aatcgtcaga tttgtatact
     7141 tggcactttt actctgtgct gctagaaggg acttcagagc aaggaataat gtgggaatgt
     7201 cctgatttat tccatttaga cggacgagat gtcttaatca tgtctccgat acaaatcaag
     7261 aagaacggac tggaatatca taatatcagt tctaccatgg cttgtattgg tgaggtaaac
     7321 tgggaaacag gtcagatgat agttgataat ttccatgaaa tggattttgg aatggatttt
     7381 tatgcaccac aaacaacaag agatcctaat ggtggacggg tgatgattgc ttggatgcag
     7441 atgtgggatc gtacattgcc tacacatgag ttaggccatc tttgggcagg ctctatgagt
     7501 ttgccccgag agttaagagt gaaaaaaaat cgattggtac aaaaacccat ctcaactgta
     7561 tatagtcatt tagtttatca acaaggcagt gaaaatattg cagtgactga cacatcagtc
     7621 gtatttcgaa acgcgctgtc tgattcttca tatttacatc tagtcgcaga tttggagaaa
     7681 gctgaagttt ttacaattga attactgcgg tctaagacgg attgtctcaa actagcctat
     7741 gacactgcta cacaggtttt tgaattggat cgtcagaaac taggaagaga gcttacaggg
     7801 gcggaagaag accctctgaa tgtacgaaaa gttgaagtac ctttgattga taatcagctc
     7861 gttcttgaga tttttagaga tacctcatca attgaagtct ttattaatgg gttagaagtt
     7921 atgacggcta ctttctatga gctggaaaaa gctcgagata tcttgtttca ttcgatcgga
     7981 gaaagtcgta ttcaagcttt tgaaacagcg agtgtgaaat aaatgtttga ttgcttaggc
     8041 gatgaatcaa ttatttaata ggttttgaag gataatgttt ttagaaaaga acagatagct
     8101 agatttccca tggatttgct attagtattt tgaaaaaatt agtaatttat gacaaaattc
     8161 attatactaa acttgtgcag aaatgtgagg gatcaactat gaaagcaaca atgaaagatg
     8221 tcgcaaaatt agcaggtgtt ggagtaggaa ctgtttcccg ggtattgaat aacggttctg
     8281 ttaaagcttc tactagagaa aaagttgaag cagcaatgac agagttaaac tatcaaccag
     8341 atttttatgc ccgggggtta aagatgaagc atacgaacac ggttgctttg attctaccca
     8401 cggtatggca tccatttttt ggcgaatttg cctaccatgt agaaacagca ttagaaagac
     8461 aaaactacaa gctttatttg tgtaactcca gtggtaatag tgaaaaagaa caagaatata
     8521 tccagatggt cagccaaaat aaagtagacg gcatcattgg aattacttat tcagatatcg
     8581 atgaatacat ttcgagtaat ctgccttttg ttagtattga ccgttatttc actgaagagg
     8641 tgatttatgt gaccgctgac aatgcggccg gtggacgttt ggctgcaaat gaactttatc
     8701 gccgaggatg tcagaatgta gcatatatcg gtgggtacca agaaacaccg aatgaaacga
     8761 agagtcgtcg aaaatatttt gaaatcgagt gccgtcaact ggggatttcc tataaagcgt
     8821 tagatatgtt agaaccttta gaccatctct cagaacgtgt aacaaatttt ttgttagaga
     8881 atcccgatat cgatgggatt tttacgatca acgatccaat ggcattagct gtcattgata
     8941 tgcttgaaaa aatagggaaa aacgtaccag aggatgtcca agttatcgga tttgatggtc
     9001 aaaagatgac agaaggagat ccttatctag tctcaacgat tgcccaacct acaaagcaaa
     9061 tggcggaagt ggcagtggca aaattaattg ctgtcattaa tggagagaca atagaaaaac
     9121 gtaccgtatt gccgattgaa tttggcgaag gtgacacaac gaaaccttta gtgagaaacg
     9181 attttgctta gtgcaaatac gtttcttttt ttgaaaatga gtgaatgaaa ggtgtagata
     9241 aaatgttata tggtggaata gaagccggtg gaacaaagtt tgtctgtggg attggtaatg
     9301 aacaattaca agtgatagaa cggaaaagtt ttcctaccac aacaccagaa gaaacgatgg
     9361 agcaagtgtt tgatttcttt gataagtaca aaggaaatat cgcatcaatt ggtgtaggag
     9421 catttgggcc tattgatatt caaatcgaat caagaactta cggatatatc accaacacac
     9481 cgaagttagc ttggcaaaat tatcattttg taggagcttt ggaaaacgcc ttgtcggttc
     9541 ccatctattg gactaccgat gtaaatgctg cttgttatgg agaatatatg gcagggtatg
     9601 gaaaaggtaa aaatagtgtt ctgtattaca ccgttggtac aggtgttggt ggtggtgctg
     9661 tagtggatgg gaaaatagtc gcaggtttta gtcatcctga aatgggacat atgctcttac
     9721 aacgtcataa tgaagatcaa tttgaaggaa tctgtccttt tcatcatgat tgtttggaag
     9781 ggttagcatc aggacctgcg attgaaaaac gctttggaag aaaagcccag actatctcat
     9841 ttgatcatcc ttattggacg attgaggcag attatcttgc tcagtgtgtg tataacacaa
     9901 cgttactata tgcaccggaa gtaataattt taggcggggg tgtaatgaaa cagccgcaac
     9961 tactcgagtt gattcgccaa tcgtttaaag aaaaaatggg ttcctacgtg gatacgccta
    10021 ctctcgaaga atatctacta actccaaggc ttgctgacaa tgcgggcttg attggctgcc
    10081 tagctttagc aataaagaaa ctcaatcatt agaatagtat ttattagaac tattcataac
    10141 ataccggaag tttaaaaggg tattctgaaa cttaaatagt ctcaattcat tcttctttct
    10201 tgagaatctg agtagagact tttattctgt ttcaaattaa tataattacg aaatgatatt
    10261 gagtacaaga aagttctttt taccagatct tttaaatcga aaaatctgat atatatattt
    10321 atagtactaa aaatctgatt agataaaaag ctaattcaat aggaaaataa aggtagtgaa
    10381 taaaagcaaa gcccttgtta gtagttaatc taacaagggc tttggtaggg aaaatggtag
    10441 gaaaccatct tattttttac gaaaatcagg aactttagta attgaataat gttgatttat
    10501 aggtattcct catccatatt atgttggctc tttattccca ctccactgtt gctggtggtt
    10561 tagaagtgat gtcatacacg atacggttga cgtgggctac ttcgtttacg atacgtacag
    10621 agattttttg caagacttcc catgggatac gggcaaagtc agcggtcatt ccgtcgattg
    10681 aagttacagc acggataccg actgtgtagt cgtaagtacg tccgtcaccc ataacaccga
    10741 cagagcggat ccctggaaga acagtgaaat attgccagat atcacggtca agtccagcag
    10801 ctgcgatttc ttcacgcaag atagcatcag attcgcgtac gatttctagc ttgtcttcag
    10861 tgatttcgcc aagaacacga ataccaagac caggaccagg gaatggctga cgccaaacga
    10921 ttgaatcagg catgcctaac tgagtcccta attcacggac ttcgtctttg aataatgtgt
    10981 ttaatggttc gatcaattca aattgcatat cttctggcag tccacctaca ttatggtgag
    11041 atttgatcgt ctgtgctgtt tctgtaccag attcgataac gtcagtatac aacgttcctt
    11101 gtgctaggaa agagacgcct tcttcaccag caagttttgt tgcttcgtca tcaaaaacat
    11161 atacgaattc attcccaatg attttacgtt tttgttctgg gtcagataca cctttcaact
    11221 tgtctaagaa gcgatctctt gcattgactt tgatgatgtt caagccgaat tttccgccta
    11281 gactttccat tacttgttcc gcttcgcctt tgcgtagcag accgtggtca acaaagatag
    11341 atgtcaattg gtcaccgatc gctttttgta aaagaacccc aacgacacta gaatcaacgc
    11401 cacctgaaag accaagcaag acttttttgt caccaacttg ttcacggatt ttttctactt
    11461 gcatatcgat aaagttatcc attgtccagt taccttcaca gtgacaaaca tcaaaggcaa
    11521 agtggcgcag caattcgatt ccgtattcgg aatgacgtac ttctgcatgg aattggatac
    11581 cataaaaatt acgtgttgtg tcttggatag aagcaatcgg acaatctttg cttgttccga
    11641 ctgtttcaaa gccttcagga actttagtta ccaagtcacc atggctcatc catactgttt
    11701 gttgttttgg tgtttcttta aacaagacag cttgatcgct tgttacttct aacaaggctt
    11761 tcccatattc gcggttttta gcaggctcta cggaaccgcc aagtttgtac gtgatcaatt
    11821 gcataccata gcaaatccct aatacaggaa tacctaaatt gaagatctca ggatcgatcc
    11881 caaatgcatt ctcatcatat acactgtttg gtccgccaga aagaatgatt ccctttggat
    11941 tcatcgcttt tacttcttct gcagtaatgc ggtggctcaa taattcggaa aagactccaa
    12001 gatcacgaat acgtcttgtg atcaattggt tatactgact accgtagtct aatacgataa
    12061 tcttttcaac gttcgtcata tcggcaacgt ttgtcaaatt tattacccct atccctttaa
    12121 attttatctc aaaatagtca aactatttta aaatgctaat attttcgcat gaaaatcgaa
    12181 tcgatgatat gattttcagt tttatggaga attatatcgg ctcttcctcg tgtaggcaag
    12241 atatattcct gtaaattttt caaattcaca tttttccata catcttttgc cattttaaag
    12301 gcttcttcac gatctccgat agcaaaacga taataataat tctctggatt ttggaaagcc
    12361 gtatccagta acgcaccaaa acgatccaaa taccattttt cgatcagcac gggatcggca
    12421 tcgacataga cagaaaaatc aaaaaagtcg ctgacgtaga tttgctgatt cgcaggtaat
    12481 tgaagcgtat tgatcccctc aacgatcaag atatcaggct gctggatcaa ttcgtacttg
    12541 tctggaatga tatcatacac atcatgtgag taaacaggta cttttatctc ttcctgacca
    12601 gattttacac ggttcaagaa atcgatcagc atctccatat cataactttc aggaaatcct
    12661 ttacggtcca tgattccacg ctcttttaat atacgatttg gataaagaaa tccgtcagtc
    12721 gtgatcagct gaacgtttct tcgtttgaag gtacgagaca aaatcgtctg cagcagtctg
    12781 gctgttgtac tttttccaac ggctacactt ccggcgatcc cgataataaa aggcggtact
    12841 tctacgtatt tatgaagaaa taaaccttta ctcaagaaca aagattcaaa ttctttcata
    12901 taaagataaa tcagatgagc aagaggtaca tagatatctc gaacatcctg catggaaatg
    12961 cggtcattca aacttttgat attatctagt tcatcttgtg tcaaaggtgc ttgcccatta
    13021 cgataaaatt ctcgccattc ttctcttgcc acacggtagt agttcattgg atcattcata
    13081 ttagcctcct gcgttctccc atcaatggaa ctattttaac acaaaataaa cgcaaacccc
    13141 accacttaag agagattgcc gatctttttg tataatcttt ataatagaaa gaaacggagt
    13201 cttattcttg acttgcgcac ggatacataa aaggatagac tgttgtcatg aaagaaatga
    13261 gaaggatgga agacatgaca aatcattact attctgaacg cccagatacg gcgcacgatt
    13321 ttgaacaatg ggcttttgag ttaaaaggca gaacctttca atttgtaaca gattctggtg
    13381 tattttcacg tgaaacagtg gattatggtt cacgtgtcct gattgatgct ttcgattggg
    13441 aagagttacc tgatgaagga aagatcttag atgtgggatg cgggtatgga ccgattgggt
    13501 tagctttggc atttgcgaca cagcggtttg ttgaaatggt cgacattaat gaacgagcgg
    13561 taagtctagc gcaaggaaac gcaaagcgta accaaatcga acatgttgat atccatcaat
    13621 cgaatatcta tgaagcagtt catgaagaaa cctatgcagc tatcgtaagc aatccgccga
    13681 tccgtgctgg aaaaaaagtt gtacatgaaa ttttgacagg cgcatatcct cggttgaaga
    13741 agggcggtac actgacaatt gtgatccaga agaaacaagg agcgccgagt gcgcaaaaga
    13801 aaatggaaga aacatttggt aatgcagaaa tcgtcacaaa agataaagga tattatatca
    13861 tcaaaagtgt gaaagaataa ctagctgaaa aatagtggag cgacgaaaga gtggaactgg
    13921 gcgtgcatgt acgggtacgt atcagttctt tttttgttga gaagttttta aaaacaaaga
    13981 actatttttt cttcaaaaag aaatagtagg aacagagtcg ttgtttttta cgataaaata
    14041 aataagatac agaacttgta tagaaggctg ccaagtcggt agataaagga ggtttttatc
    14101 aaaaaacata ctttcctttt ttctctattc ttttcttggg ggttttggta cactgatagg
    14161 gaatttagat agaagggagc caataggatg aagctgacca tcagagatat cgcagaaatg
    14221 gcaggcgtat cagtaacaac tgtctcacag attttgaata acaaaggaag cagatttagt
    14281 gataagacaa gaaaaaaagt gttgtcgatc gtgaatgaac atcattataa gcccgattat
    14341 ttcgcttcaa atgtgatcaa tcgacattca aagaccatcg gaatgatcgt ccctgatgtc
    14401 actgatttct tcttctccaa actgatcgaa ggtgtagaga gctatttgaa tcctttgggc
    14461 tatgtgatca tgttatgcaa ttctcgtcat agccaagaaa atgagatcaa gtatttacaa
    14521 gagctttccc accgctcagt ggacggaatc ttgctggcca caccaaatat tttacctgag
    14581 aaatatgcat tagacagcgg tttttatcaa aaaatgccaa tcattttaat cgatcgcgga
    14641 ttgaatcgtc gggataacgg acggcttatc gtaaaggaat atgagggcgc ctatcaagct
    14701 gtttcgctgt ttattgaaaa tggccatacg aagatcggca tgctgaagga aaccactgga
    14761 tactatcaat tagaagaacg tttcaacgga tatcgtcatg cgctgaaaga tcatggcctg
    14821 ccttttaatg ggaaatttgt tgaacatgga gagttgactg tccaaggtgg atatactgca
    14881 tcaaaaaaat tactcaaaca taaagatatc acggctattt tttgcgggaa tgatgccatg
    14941 gcgatcggat gctatcaggc gatcgatgaa ttgggcaaaa aaatcccaga agatatctca
    15001 gtcataggat tcgatggatt aaagctgtca gaatacatga ttccgcaatt gacgacagtg
    15061 cagcagccaa gttttgatat tggattttat gctgcacgtt tcctgatcga tacgatcgaa
    15121 tttcctcaaa gaaaagttcc gaacaaagtg ttcgaaacaa aattaattat tcgtgagagc
    15181 gtaaaagtat tgacaaaggg ctaactcggc ggtatattgc ttatgtaagc atttacaaag
    15241 gctagttagt gcttttttta tttttagtaa accagtttac taaagtggtt tacctttcct
    15301 aaaaataatg tataataccg aatgaagaca aggaggatca ggcaatgcga atggtggatc
    15361 tgattgaaaa aaagagggat ggaaacgagc tgtcgaaaga ggaaatcgag tacatcgtca
    15421 cgaactatac gaatggaaag attccagatt atcaagtcag tgcattattg atggcaattt
    15481 tttatcaaga catgacaaat gaagaaatca cgaacttaac attggcgatt gctaattctg
    15541 gtgatgtcat cgatttgagt tctcttgaag gaataaaggt agataagcat tcgacaggtg
    15601 gcgttggtga cacgaccaca ttgattttag cccccctagt agctagtgtc ggagtgacag
    15661 tggctaaaat gtctggacgc ggtctaggtt acacaggtgg tacactagac aagctagaag
    15721 caattcccgg atttcagatc gagctttcgg atgaagaatt tgtacgcatt gttaatgaaa
    15781 gtaaagtagc ggtaattggt caatcaggaa acttggcacc ggcggacaaa aaattgtatg
    15841 ccttgcgtga cgtcacagcg accgttgatt ctcttccgct gattgccagt tcgattatga
    15901 gtaaaaaaat cgcggcagga gcagatgcaa tcgttttgga tgtgaccact ggagacggtg
    15961 ctttcatgaa gaacatcgaa gatgcaagac gtctggcaaa gaccatgacg agcattggga
    16021 aactggctaa ccgagaaaca gtggcggtta tctcagatat gtcagaacct cttggcgaag
    16081 ctatcggaaa tagtctagaa gttgtcgagg caatcgaaac attacaagga aatggtccag
    16141 aagatctagt agaaatgtgc tacgccttag gaagccaaat ggttgttctt gctggcaaag
    16201 ctaagacaac cgatgaagca agaacgttgc ttcaagaagc tctggaatca ggtaaagcgt
    16261 tagcaaaatt caaagaaatg atccaaaatc aagggggaga cccagcgatc gtggaacatc
    16321 cagaacgaat tttgactgca cgctacacaa tggagcttcc tgctaaacaa agcggagttg
    16381 tgtcaaaaat cgtggcaaat gaattaggaa ttgctgcaat gatgctaggt gctgggcgta
    16441 aaaccaaaga agaagatatt gatcacgctg ttggattgaa actgcataaa aaaattggtg
    16501 acactgtgac aaaaggtgag tcactattga cgatctatag taacgacgaa gaaatcactt
    16561 ctgtcattga attattatat aaaaatattg aaattggtga atcggcaatg aagccaacgt
    16621 tgattcatga catcatcaca gaataaagga aaaaagcgga ctttaccgtt ttaaagaaaa
    16681 ggggaaaaga aatggaacta aaccgaatga tcgaccatac tattttgaaa gcagacgcat
    16741 ctaaagaagc tgtgatgcaa attatcgaag aagcaaaaaa ataccatttt tattctgtct
    16801 gcatcaatcc agcatgggtt gcgctagcga aagaacaatt acaaggtgag ccagtagccg
    16861 tatgtacagt aatcgggttt cctctaggag caaacacacc cgagacaaaa gcatttgaaa
    16921 caacagatgc catcaataac ggtgcagatg aagtcgatat ggtcatcaat atcggtgctt
    16981 tgaaatcaaa ggatgatcaa caagtccaaa aagatatcga agcagttgtt gaagcagcaa
    17041 aagatcgtgc gcttgtcaaa gttattattg aaacatcgtt attagataga gaagaaatca
    17101 tccgagcttg cgaaatagca aaagctgcag gcgctgattt tgtcaaaaca tcaactggtt
    17161 tctcaacagg cggagcaaag gtagaggatg tccgtttgat gcgtgaaact gtaggaccag
    17221 aaatgggtgt caaagcttcc ggcggtatcc ataacgaaga agaagcaatg gcaatgatcg
    17281 aagctggtgc tacaagaatc ggaacaagcg caggagtagc aatcgtatct ggttcaactg
    17341 gtgaaggcta ttaatttttt caaaggagaa gctcatggca gaactcaaac aagaatggat
    17401 caacacagct attgaagcgt tagataaagc ctatgttcct tattcccatt ttccggtagg
    17461 cgcttgtctc gtaacggaat caggcaagat ttatcaaggc atcaacattg agaatgcatc
    17521 atttggatta acgaattgtg ccgagcgcac agcttttttc aaagcagtat ctgaaggaga
    17581 aaaaacattt acgcatctag tcgtagcggg gcacaccccc gatcctatct ctccttgtgg
    17641 tgcatgtcga caagtaatgg cagaattctg ttcccctgat atgccggtta cgctagtagg
    17701 agacaatggt gtgacgaagg caacgaccgt tcgtgcactg ctgccttatg catttactga
    17761 aaaagatcta taactcatgt tcattcagtc agctgactgt tgaaaaataa aacaattatt
    17821 ttaggaggct ttacagaaat gaaaaaagca aaattatttg gtttaggtgc tgtcgcacta
    17881 gcagcagggt tatttttagg tgcatgcggc aacaacggct ctactgattc atccggtggc
    17941 aaaagctcat cagacacaac aactgcagca ttgatcacag acacaggtgg tgtagacgac
    18001 cgttcattca accagtcagc ttgggaagga ctagaaaaat gggggaaaga tcaaggactt
    18061 tcacgaggaa atgatggctt ccaatatttc caatcttcaa atgaatcaga ttacatccca
    18121 aatattgacc aagcattgaa tgctggattc aaaacgattt ttggtattgg ttataaacta
    18181 aaaccagcga tccaagaaca agcaacaaac aatacaggta cgaactttgt tattatcgat
    18241 gatgtcatcg atggattaga taacgttgta tctgcaactt tcaaagacaa cgaagcttct
    18301 tatcttgcag gtgttgcagc agcttatacg actgaaacaa acgtagtcgg ctttatcggc
    18361 ggtgtaaaag gtgaagtaat cgatcgtttt gatgctggat tcaaagccgg tgttgatgca
    18421 ggtgcgaaag aattaggaaa agaaatcaaa gtattgaatc aatatgctgg tgacttctct
    18481 gctccagata aaggacgttc aattgctcaa ggtatgtatg cacaaaacgc agatatcatc
    18541 ttccatgctt caggcggtac aggtaacggt gtattccaag aagcaaaatc attgaacgaa
    18601 tcaggcgaca aaaaagtttg ggttatcggt gttgaccgcg accaatcaga cgaaggcgaa
    18661 tacacattaa acggcgaaaa gaaaaacttc acattgacat ctacactgaa agcagttgga
    18721 acagtagtag aagatttggc acaaaaatca gcggacggta aattccctgg cggcgaacac
    18781 actgtttatg gattgaaaga agacggtgtt ggtctaacag aaggacaact ttctgacgaa
    18841 gcgaaaaaag cagttgatga agcaaaagaa aaaatcattt caggtgacgt aaaagttcca
    18901 gaaactcctg aagaaaacta aaaccatttc tatagccttg ttagacacat gtttgacaag
    18961 gctatggttt gattagaaca agaattgttc atttatccaa gctgccttgt gacagtttgg
    19021 ataagggaac aactccctga agtaatgttt gtttcaaaag aagaacgctc actaagcgtg
    19081 aatcttagtc aatcgttgcc ctagatagaa atcgttagga tgtgaaaaaa aggtggttca
    19141 agaaaatatt gtcatagaaa tgcagaatat taccaaagaa tttggaacat tcaaggcaaa
    19201 tgacaacatc aatttacaag taaaggcagg agaaattcat gcgttgcttg gagaaaacgg
    19261 tgctggcaaa tctacattga tgaacgtgct ttccggatta ttagagccga catcagggaa
    19321 aattttgatg cgtgggaaag aagtaaagat cacaagtccg accaaagcca accaattagg
    19381 gattgggatg gtccatcagc actttatgct tgttgatgcc tttactgtaa cagaaaacat
    19441 cgtgttggga agcgagccta gtcgcgcagg gatgcttgac cataaaaaag cacgaaaaga
    19501 gatccaaaaa gtttctgaac agtatggatt gtcagtcaac ccagatgctt acgttcgtga
    19561 tatttcagtt ggaatggaac aacgggtaga aattttaaaa acactttacc gaggagcaga
    19621 tgtactgatt tttgatgagc cgacagctgt attgacccct caagaaattg atgaattaat
    19681 cgtgatcatg aaggaattag tcaaagaagg caagtcgatc attttgatta cgcataagtt
    19741 agatgaaatc aaagcagtag ctgaccgctg tacagttatc cgccgtggaa aaggaatcgg
    19801 tacagtcaac gttaaagacg taacctcaca acaattagcc gatatgatgg ttggaagaac
    19861 ggtttcattc aaaacgatga aaaaagaagc gaagcctcaa gaagtcgttt tgtctattga
    19921 aaatctagtg gtgaaagaaa atcgtggatt agaagccgtg aaaaacttga acttagaggt
    19981 tcgtgctggc gaagtacttg gtatcgctgg aatcgatgga aacgggcagt cggagttgat
    20041 ccaagctttg actggtttgc gaaaggcaga aagcggacat atcaagctaa aaggggaaga
    20101 catcactaat aaaaaacccc gaaaaatcac tgaacatggt gtaggacacg tgccagaaga
    20161 ccgtcataaa tacgggttag tcctagatat gacattatct gaaaatatcg cactacaaac
    20221 ctatcatcaa aaaccttaca gtaaaaacgg catgctgaat tattcagtaa tgaatgaaca
    20281 tgccagagaa ttgatcgaag aatatgatgt tcgaacaacg aatgaacttg ttcccgcaaa
    20341 agctttatca ggcggaaatc agcaaaaagc aattatcgct cgtgaagtcg accgagatcc
    20401 tgatctgttg attgttgcaa atccaactcg tggactggat gtaggtgcga tcgaatttat
    20461 tcataaacgt ctgatcgaac aaagggacaa atacaaagca gtgttactga tcagtttcga
    20521 attagaagaa attttaaatg tttcggatcg tattgctgtt atccatgaag gagaaattgt
    20581 cggtatcgtt gatccgaaag aaacatctga aaatgaatta ggtctattga tggcgggcta
    20641 taccttagaa gaagcacgaa aagagttaga aaagggggct ggcgcacatg aatgatcgca
    20701 gcgaaaaatt ccgtaattta ttagtgcctg tactttcagt tgttttagga ttgatcttag
    20761 gcgcgatatt gatggctgct tttggttttc agcccatcca aggttattct tctatgctag
    20821 atgcagccct tggttcgcaa agagggatag gtgaaacgtt aaggcaagca acgccgttga
    20881 tttttacagc attaggtttt tctgtagcta actcagctgg gtttttcaat attgggcttt
    20941 ctggacaagc actttgcggg tggatcgtca gtgtctgggt ggcacttgcg tttccagatc
    21001 tgccaaaagt tgttttgctt ccgttatgcg tgatcttagg tgccttagct ggtgcgatgg
    21061 cagcggctgt tccaggttta cttcgagcat tttttggaac aagtgaagtg atcgtaacaa
    21121 tcatgatgaa ctatatctta ttatacgtaa gcacatttgc tttacatgaa gtgatgcctg
    21181 aaagctatcg ctcaagtttg gactcttcca atatgatcag tcaaaatgcc tcactgcggt
    21241 taccatggtt aacacaagct tttggcggct ctcgcgtaaa ctcaggatta tttttagcaa
    21301 tcatcgcgct tattttcgtt tggctgctga tgaagaaaac gactttagga tttgaaatcc
    21361 gttctgttgg attgaatcct tttgcttctg agtatgcagg aatgagtagt aaacgtacga
    21421 tcgttttgtc gatggtcatt tctggcgctt tggcagggct tggcggtgtg gttgaaggat
    21481 taggaacgta tcaaaacttc tttgttcaaa cgacttcgct atctattgga tttgacggaa
    21541 tggctgtttc cttgcttggt tctggatcag caatcggtat tcttttatca gcgatcttgt
    21601 tcagtatttt gaagatcggt ggacttggca tgcagacagg agcaggtgtt ccgtttgaga
    21661 tcgtcaatgt atcgattgct ttgattatct tctttgtcgg gatcaacttc ctgattcgtt
    21721 tcgtcatggc taaagtcttc caagggaaaa aacaagaaga gatcgttgcg acaatagaat
    21781 cgaaaccaac gaatcaaaac catgaagggg gcgaactata atgtcgacta tcgaattaat
    21841 tgccttgatc cttacttcta cactggttta ttcaacaccg ctaattctaa cgtcactagg
    21901 cggaactttt tctgaacgaa gcgggatcgt aaacgtaggg ctagagggaa tcatggtcat
    21961 gggtgctttt agttccatcg ttttcaactt gacgatgagt ccatcacttg gttctatgac
    22021 accatggatc gctattttag tgggtggcgt cgtaggtatc ctattttctc tgattcacgc
    22081 agtagcaaca gtcagtttgc gtgctgacca tattatcagc gggacagtaa ttaatctgat
    22141 ggcacctgct ttaggtgttt tcttgatcaa agcatggtat ggtaaaggtc aaactgataa
    22201 catcacgcag aatctggggt atttttcttt cccaggatta gcagatattc cggtcattgg
    22261 accaattttc tttaaaaata cattcttacc tgcttattgt gctattttga ttgctgtcct
    22321 agcttgggtg atcatcttca agacgaaatt cggcttgcgc ctccgttcag ttggtgaaaa
    22381 tcctcaagct gccgacacat taggtatcaa tgtatatgct atgcgttatt caggcgtact
    22441 gatttctggc ttgttaggcg gtatgggcgg tgctgttttt gctcagtcga tttccggtaa
    22501 cttttcagct ggtacgatcg ttggacaagg gttcatctcc atggctgcta tgatctttgg
    22561 taagtggaat cctttagggg caatgggtgc ctcattgttc tttggttttg ctcaaagctt
    22621 gagtatcatt ggcgcacaat taccagttat ttcttcgatt ccgccagtat tgctgcaaat
    22681 tgctccatat ttgctgacaa tcattgtgct ggttgttttc ttagggaaag cttctggtcc
    22741 aaaagcaaac gggaagaact atatcaaatc aaaataatct tttagcgaat aaaaagggtg
    22801 ggttacggga agtgtttttc gtctcgccct ttttgtttta aaggcaataa aaataaaaag
    22861 aaagaaggaa taatcatgtt taaacgcgta catctgatcg tcatggattc agtaggaatt
    22921 ggagaagcac cagatgccga taagttcggt gatgttggtt ctgatacatt aggacacatt
    22981 gcgaaagaag caggattgaa cgttccacat ttagagcagc ttggtttagg aacgatccgt
    23041 cctcttgaag gtgtggaatc agtgaaagat catcaaggct atgcgacgaa attagaagaa
    23101 gtttctgtag ggaaagatac gatgacaggc cactgggaaa tcatgggatt gaatatcaaa
    23161 actccatttc gtgtgtttcc taatggattc cctgatgatc tgatcaaaca aatcgaagaa
    23221 ttttctggcc gtaaagtcgt atgtaacaaa ccttatagcg gaacagctgt tattgacgat
    23281 tacggtgaac accaaatgaa aacaggcgat ttaattgtat atacttctgc cgatcctgtc
    23341 cttcagatcg cggcacatga agaaatcatt cctttagaag aattatatcg gatttgccaa
    23401 ttcgttcgtg atataacaaa agatgatccg tatatgattg gccgaatcat tgcacgtccg
    23461 tacgtgggtg aaccaggaga cttcacacga acaagcaatc gccatgatta tgcgttgaat
    23521 ccattcggac acacagtcct tgattctttg aaagaagcag gaaaggacgt gattgctgtt
    23581 ggaaagatca acgatatttt caacggacaa ggaatcactg aatctgtccg cacgaaaagc
    23641 aatatggatg gcgttgacca gctgctgaaa gttatgaaaa acgatttttc aggaatcagc
    23701 tttaccaatt tagtagattt tgatgcatta tacggtcatc gtcgggatgt agtcggctat
    23761 gcgcatgcga ttgaagaatt tgacttacga attccagaat tgcttgaagc gatggaagaa
    23821 gacgacttgc taatgattac agccgatcac ggaaacgatc cgacgtttac tggtaccgat
    23881 catacgcgag aatatgtacc gttattggtt tacagtaaaa aaatgaaaga acaaggggca
    23941 ttgcctcaag gatattattc agatatttca gcaacgattg ctgagaactt tagtgtttct
    24001 tcgactgaaa atggcgaaag cttcttgaaa ttattaaaat aatcttttta aataggagaa
    24061 agcaaatgac caagttaagc gagatgttac aagaaacgac ccagtttatc cttgataaag
    24121 gagtaaaaga aattgatttt ggtttgattc taggttcagg attaggtgaa ttagcagatg
    24181 aaatcgaaga tgctgtaatg atttcttatg atgagatccc gttctttcct gtttcgacag
    24241 tagttgggca tgcaggaaag ctagtttatg ggtctctggc aggcaagaaa gtgttagcca
    24301 tgcaagggag attccactac tatgaaggtc attccatgca aacagtgacc tatccagtac
    24361 gtgtgatggc cggactaggg gcccattcag tcattgtgac taatgcatgt ggtggtgtca
    24421 acgaatcatt tgtaccagga gatttgatgc tgattaccga tcatatcaat tttactggtg
    24481 aaaatccttt gatcggtccg aatgaagatg caatgggccc tagatttcca gatatgagtc
    24541 aagcttatac gcctgactat caagcaacag caaaagcagc agcagaaaaa ctaggtttgg
    24601 atcttaaaga aggcgtatat atgggctatt ctggaccaac ttatgaaacg cctgcggaga
    24661 tccgaatgtc tcggacaata ggagccgatg cagtagggat gtcgactgtt ccagaagtca
    24721 tcgtagcagc tcacagcgga ttaaaagtgt taggaatctc ttgtattacc aacttagcag
    24781 caggtatgca agcgaatctg agccatgaag aagttgtaga aacaactcag agagtcaaac
    24841 aatcttttaa agcattggtt aaagaaacat tgacattatt gtaataggag gaacaataaa
    24901 atgagtgtgc atattgaagc gaaagaaggc gagatcgccg ataagatttt acttccggga
    24961 gatcctttgc gagctaaata tattgcggag acttttttgg aagatccagt ttgttacaat
    25021 caggtacgcg gtatgttagg ctttaccggc aaatataaag gagaacgtat ttctgttcaa
    25081 ggaacaggaa tgggaatgcc atcagccacc atctatgccc atgaattgat ccagtcgtat
    25141 ggtgtaaaga aattgattcg agtaggtaca tgcggtgcat tgtcaaaaga cgtccatgtt
    25201 cgtgatttag tactagcaca aggtgcagcg accagttctt cgatgatcga aaaaaatttc
    25261 caagcctttc attttcctcc aatcagtgat ttcaatctat tactgaaagc atatgagata
    25321 gctaaagaaa agggctatac ggttcatgta ggaaatgttc tttcggaaga ttctttctac
    25381 aaagatgatt tgacagagac gttccaatta gctgaacttg gcgttttagg agtggaaatg
    25441 gaagcagcgg ctctttatta cttaggcgcg aaataccatg tgcagacatt aagtttgatg
    25501 accgttagcg accatttgat caccggtgaa gaaacaacag ctgcagaacg tcaatctacg
    25561 ttcaatgaaa tgatcgaagt aggattggaa acagcgatag cagaataaga aggaaatgaa
    25621 aaagctgccc tgcattaagt aataagaaca tccaagcaaa aatagctcct catgtttttg
    25681 acttggctgg acttattatt gcaaatgtaa gcttttgaac accgtttacc aagaggttgt
    25741 gaa
//
LOCUS       DACDYI010000046        25673 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.046, whole genome shotgun sequence.
ACCESSION   DACDYI010000046 DACDYI010000000
VERSION     DACDYI010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.046"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            19..936
                     /locus_tag="IWM52_001749"
     CDS             19..936
                     /locus_tag="IWM52_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9973998.1"
                     /translation="MPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILT
                     FLTGISSRDGERFPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTD
                     YDFAHLLFSNGYVEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVIS
                     TFALDFIHTDRSIFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDD
                     LLDEKLKNNLGLKFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLY
                     GRVGVIQFHPVFEELLHAFDTRPPVRKEV"
     gene            939..3116
                     /locus_tag="IWM52_001750"
     CDS             939..3116
                     /locus_tag="IWM52_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP9973999.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            3113..4114
                     /locus_tag="IWM52_001751"
     CDS             3113..4114
                     /locus_tag="IWM52_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP9974000.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            4111..5043
                     /locus_tag="IWM52_001752"
     CDS             4111..5043
                     /locus_tag="IWM52_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9974001.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            5288..5404
                     /locus_tag="IWM52_001753"
     CDS             5288..5404
                     /locus_tag="IWM52_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP9974002.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            5420..7339
                     /gene="tet(M)"
                     /locus_tag="IWM52_001754"
     CDS             5420..7339
                     /gene="tet(M)"
                     /locus_tag="IWM52_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAP9974003.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNVCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            7458..7625
                     /locus_tag="IWM52_001755"
     CDS             7458..7625
                     /locus_tag="IWM52_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9974004.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(7685..8038)
                     /locus_tag="IWM52_001756"
     CDS             complement(7685..8038)
                     /locus_tag="IWM52_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9974005.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSIM
                     ESLASGINEARNIED"
     gene            8543..8965
                     /locus_tag="IWM52_001757"
     CDS             8543..8965
                     /locus_tag="IWM52_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9974006.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            8962..9192
                     /locus_tag="IWM52_001758"
     CDS             8962..9192
                     /locus_tag="IWM52_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9974007.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            9653..9856
                     /locus_tag="IWM52_001759"
     CDS             9653..9856
                     /locus_tag="IWM52_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAP9974008.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            9938..11155
                     /locus_tag="IWM52_001760"
     CDS             9938..11155
                     /locus_tag="IWM52_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9974009.1"
                     /translation="MSEKRRDNRGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
     gene            11387..11713
                     /locus_tag="IWM52_001761"
     CDS             11387..11713
                     /locus_tag="IWM52_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP9974010.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            11781..12494
                     /locus_tag="IWM52_001762"
     CDS             11781..12494
                     /locus_tag="IWM52_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP9974011.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            12804..13655
                     /locus_tag="IWM52_001763"
     CDS             12804..13655
                     /locus_tag="IWM52_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9974012.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            13776..15641
                     /locus_tag="IWM52_001764"
     CDS             13776..15641
                     /locus_tag="IWM52_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9974013.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            15689..17098
                     /locus_tag="IWM52_001765"
     CDS             15689..17098
                     /locus_tag="IWM52_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAP9974014.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            17206..17907
                     /locus_tag="IWM52_001766"
     CDS             17206..17907
                     /locus_tag="IWM52_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9974015.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            18066..18545
                     /locus_tag="IWM52_001767"
                     /pseudo
     CDS             18066..18545
                     /locus_tag="IWM52_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            19393..19968
                     /locus_tag="IWM52_001768"
     CDS             19393..19968
                     /locus_tag="IWM52_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAP9974016.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            19968..20408
                     /locus_tag="IWM52_001769"
     CDS             19968..20408
                     /locus_tag="IWM52_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP9974017.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            20424..21125
                     /locus_tag="IWM52_001770"
     CDS             20424..21125
                     /locus_tag="IWM52_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP9974018.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(21613..22038)
                     /locus_tag="IWM52_001771"
     CDS             complement(21613..22038)
                     /locus_tag="IWM52_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974019.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            22412..23383
                     /locus_tag="IWM52_001772"
     CDS             22412..23383
                     /locus_tag="IWM52_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9974020.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            23415..24812
                     /locus_tag="IWM52_001773"
     CDS             23415..24812
                     /locus_tag="IWM52_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9974021.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            24971..25594
                     /locus_tag="IWM52_001774"
     CDS             24971..25594
                     /locus_tag="IWM52_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974022.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
BASE COUNT         8649 a         4321 c         5404 g         7299 t
ORIGIN      
        1 ggcagatgga aagaaacctt gccagagatt tcccatgaaa tcaatatcgt cactctgact
       61 tctgatgaga aaaacaaagg cttacttgac ccttatgtga ttatgaaaaa tcccaaagat
      121 tctgaatcac tggctattga tattctgaca ttccttacgg ggatttcctc tcgtgatggg
      181 gaacgcttcc caatccttag aaaagccatt cgtgcagtaa ccaatagtga agtacgaggg
      241 ttgatgaaag tgattgagga attacgggtt gagaatacgc cactaagtac cagtatagcc
      301 gaccatatcg aaagttttac agactatgac tttgcacatt tattattcag taatggttat
      361 gtggagcagt ctatcagctt agaaaaacaa ctgaacatta tacaggttgc ggacttggta
      421 cttcccgaca aggaaacttc ctttgaggaa tataccacta tggagctttt atccgttgct
      481 atgctgattg tcattagtac ctttgcttta gactttatcc atacagaccg aagcattttc
      541 aagattgtag atttagacga agcatggagc tttttacagg tagcacaagg aaaaacacta
      601 tctatgaagc tggttcgggc tggtcgtgct atgaacgctg gggtatattt cgtgacccaa
      661 aatacagacg acctcttaga tgaaaaactg aaaaataacc tcggcttaaa atttgcattt
      721 cgttccactg accttaacga gattaaaaag accttagcct tttttggtgt agacccagag
      781 gacgaaaaca atcagaagcg attgcgtgat ttggaaaacg ggcaatgcct tatcagtgat
      841 ttatatggtc gtgtcggtgt gatacagttc caccctgtat ttgaagaact gctccatgcc
      901 tttgatacca gaccacctgt gcgaaaagag gtgtaaatgt gaaaccatca atagtaaaca
      961 gaataaaatc aaactggacg ctgaaacgtc taggtaaagt ggcaatgaca gtggctttca
     1021 cacttgtgat tgccattttt cttttagcca tgctgggaac ggtggttcaa gctgcgggct
     1081 tggtagatga tacggtcaat gtggcaaatg aatacagccg atacccactt gaaaactatc
     1141 aactggattt ttatgtggat aatagctggg gctggcttcc gtggaactgg tcggacggga
     1201 ttggaaaaca ggtcatgtat ggactatatg ccattaccaa ttttatttgg acaatcagtt
     1261 tgtatgtttc caatgcgaca ggttacttag tacaggaagc ctattcctta gacttcattt
     1321 ccgctacagc agattccatt ggtaagaata tgcagacctt agctggtgtg agtgcaaacg
     1381 gattttcaac agagggtttc tatgttggat tcctcttact cttgattttg gttcttgggg
     1441 tttatgttgc ctatacggga ctgataaaga gagaaaccac aaaggcaatt catgccatta
     1501 tgaattttgt gctggtgttt atcctatcgg cttcctttat tgcctacgct cccgactaca
     1561 ttaaaaaaat caatgacttt tcatcagaca tcagtaatgc cagtttatca cttggcacga
     1621 agattgtcat gccccattcc gatagtcaag gcaaggacag cgtggactta atcagagata
     1681 gcctgttttc catacaggtt cagcaaccgt ggctactgct tcaatacaac agttcagaca
     1741 ttgaaagtat cggtattgac cgtgtggaaa gcctgctctc caccagccca gattccaaca
     1801 atggcgaaga cagagaaaaa attgttgcgg aagaaattga agacagaagc aataccaatc
     1861 taaccattac aaagaccatt aaccgtttag gtacagtctt cttcctattt gtcttcaata
     1921 ttgggatttc catatttgta ttcctattaa caggaatcat gattttctcg caggtacttt
     1981 ttatcatcta tgctatgttt ctgcctgtga gctttatttt aagcatgatt ccatcatttg
     2041 atggtatgtc aaaacgagcc ataacaaagc tctttaatac cattttgaca cgagctggaa
     2101 tcacattgat tattacgaca gcatttagta tttcaaccat gctctatacc ttatcggctg
     2161 gttatccgtt ctttttgatt gcttttctac agattgtgac ctttgcagga atctacttca
     2221 agctgggcga tttaatgagt atgttttctc tacagagtaa cgattctcaa agtgtgggaa
     2281 gtcgtgtgat gagaaaacct cgtatgctta tgcacgctca catgcaccgt ctacagcgga
     2341 aacttggacg ttccatgact actctagggg ctgggtctgc cattgttaca ggtaaaaaag
     2401 gacagtcggg ttcggggagt tctgcaagga cacaagcaga tcactcccga ccagacggaa
     2461 aggaaaaatc aacacttgga aaacgtatcg gtcaaaccat cggtacagta gctgatacca
     2521 aagacagaat ggtagacact gctagtggtt tgaaagaaca ggttaaagat ttgccgacca
     2581 atgcaagata tgcagtatat caaggaaaat ccaaagtaaa agagaatgtc cgtgatttaa
     2641 ccagtagtat ttctcaaacc aaagcggaca gagccagtgg acgcaaggaa cagcaggaac
     2701 aaaggcgaaa aaccattgcg aagcgtcgct ctgaaatgga acaggtcaaa cagaaaaaac
     2761 agcctgcttc ttctgttcat gaaagaccga ctacaagaca agaacaatat catgatgaac
     2821 agacctcaaa acagtctaat attcagactt catataagga atctcaacaa gccaaacaag
     2881 agcgtccagc agttaagtcc gatttttcaa gtccaaaagt ggaacgccaa ggcaataccg
     2941 ttcaagaaaa aaccgttcaa aagccagcaa cttcaaccac tacagcagat agaacttcac
     3001 aacgtccaat cacaaaagaa cgtccgtcta ctgttcaaag agtaccacta caaaatacaa
     3061 gaagtagacc accaatcaaa accgccacca ttaagaaagt cggtaagaaa ccatgaagtt
     3121 gaaaacttta gtgattggtg gttctggatt attcttgatg gtcttctcac tgcttctgtt
     3181 tgttgccatt ttattttcag atgaacagga cagcggaatt tccaatattc attatggagg
     3241 tgtgaatgtt tccgcagaag tgctggctca taagcctatg gtagaaaaat atgccaaaga
     3301 atatggcgtt gaagaatatg tcaacatact tcttgcgatt atacaggtgg aatcgggcgg
     3361 tactgcggaa gatgttatgc agtcctcgga atccctcggt cttccaccta attcattgag
     3421 tacagaagaa tccattaagc aaggtgtgaa gtatttcagt gaattattag ccagtagcga
     3481 aaggctcagt gtagatttag aatcggttat ccagtcctac aattatggtg gtggtttctt
     3541 agggtatgtg gctaatcgtg gaaataaata tacctttgaa ctggctcaaa gtttctcaaa
     3601 agagtattca ggtggcgaaa aagtgtctta ccccaatccc atagccatac ctatcaatgg
     3661 gggctggcga tacaactatg gcaatatgtt ttatgtgcaa ctggtaacgc agtatcttgt
     3721 cacaacagag tttgatgatg atacggtaca agccatcatg gacgaagcac tgaaatatga
     3781 gggctggcga tacgtttacg gtggagcttc cccgactact tcttttgatt gtagcggact
     3841 gacacaatgg acgtatggaa aagctggaat taacttacca cgaaccgcac aacagcaata
     3901 tgatgtgacc cagcatatcc cactatcgga agcacaagct ggcgatttgg ttttctttca
     3961 ttctacctat aacgctggct cttatattac tcatgttggg atataccttg gcaataaccg
     4021 tatgtttcat gcaggcgacc caatcggtta tgccgactta acaagcccct actggcaaca
     4081 gcatttagtg ggagcaggac gaatcaaaca atgagaaagg aagatttaat gatgaaattt
     4141 agaaaaaatc agaataaaga aaaacagata ccaaaggaaa agaaacctcg tgtctataag
     4201 gtcaatcctc ataaaaaggt tgtgattgcc ttgtgggtac ttttagggct tagtttcagc
     4261 tttgcgatat tcaagcactt tacagctata gatactcata ctattcacga aacaactatc
     4321 atagaaaagg aatacgttga tactcatcat gtagaaaatt ttgtagagaa ctttgcgaaa
     4381 gtctactatt catgggagca atccgataag tccattgata atcgaatgga aagtctaaaa
     4441 ggctatctga cagatgaact tcaagctctc aatgttgata cagtacgcaa agatattcct
     4501 gtatcgtctt ctgtaagagg atttcagata tggacggtag agccaactgg cgacaatgag
     4561 tttaatgtaa cctacagtgt agaccagctc attacagagg gagaaaatac aaagaccgtc
     4621 cactctgctt atatagtgag tgtctatgta gatggttctg gaaatatggt actggttaag
     4681 aatccgacca ttaccaacat acctaagaaa tcaagttata aaccaaaagc cattgaaagt
     4741 gaggggacgg ttgattccat tacaaccaat gaaatcaatg agtttttaac gacgttcttc
     4801 aagctctatc ctacagcgac agccagtgaa ctttcctact atgtgaatga cgggatatta
     4861 aaaccaatcg gaaaagagta catctttcaa gaactggtaa atcctattca caatcgtaag
     4921 gataatcaag tcacggtatc gctgacagtg gagtatatcg accagcagac caaagcaacg
     4981 caggtatctc aatttgattt ggtacttgaa aagaacggga gtaattggaa gattatagaa
     5041 taacaaatat tggtacatta ttacagctat tttgtaatca cgtactctct ttgataaaaa
     5101 attggagatt cctttacaaa tatgctctta cgtgctatta tttaagtatc tatttaaaag
     5161 gagttaataa atatgcggca aggtattatt aaataaactg tcaatttgat agcgggaaca
     5221 aataattgga tgtccttttt taggagggct tagttttttg tacccagttt aagaatacct
     5281 ttatcatgtg attctaaagt atccggagaa tatctgtatg ctttgtatgc ctatggttat
     5341 gcataaaaat cccagtgata agagtattta tcactgggat ttttatgccc ttttgggctt
     5401 ttgaatggag gaaaatcaca tgaaaattat taatattgga gttttagctc atgttgatgc
     5461 aggaaaaact accttaacag aaagcttatt atataacagt ggagcgatta cagaattagg
     5521 aagcgtggac aaaggtacaa cgaggacgga taatacgctt ttagaacgtc agagaggaat
     5581 tacaattcag acaggaataa cctcttttca gtgggaaaat acgaaggtga acatcataga
     5641 cacgccagga catatggatt tcttagcaga agtatatcgt tcattatcag ttttagatgg
     5701 ggcaattcta ctgatttctg caaaagatgg cgtacaagca caaactcgta tattatttca
     5761 tgcacttagg aaaatgggga ttcccacaat cttttttatc aataagattg accaaaatgg
     5821 aattgattta tcaacggttt atcaggatat taaagagaaa ctttctgccg aaattgtaat
     5881 caaacagaag gtagaactgt atcctaatgt gtgtgtgacg aactttaccg aatctgaaca
     5941 atgggatacg gtaatagagg gaaacgatga ccttttagag aaatatatgt ccggtaaatc
     6001 attagaagca ttggaactcg aacaagagga aagcataaga tttcagaatt gttctctgtt
     6061 ccctctttat catggaagtg caaaaagtaa tatagggatt gataacctta tagaagttat
     6121 tactaataaa ttttattcat caacacatcg aggtccgtct gaactttgcg gaaatgtttt
     6181 caaaattgaa tatacaaaaa aaagacaacg tcttgcatat atacgccttt atagtggagt
     6241 actacattta cgagattcgg ttagagtatc agaaaaagaa aaaataaaag ttacagaaat
     6301 gtatacttca ataaatggtg aattatgtaa gattgataga gcttattctg gagaaattgt
     6361 tattttgcaa aatgagtttt tgaagttaaa tagtgttctt ggagatacaa aactattgcc
     6421 acagagaaaa aagattgaaa atccgcaccc tctactacaa acaactgttg aaccgagtaa
     6481 acctgaacag agagaaatgt tgcttgatgc ccttttggaa atctcagata gtgatccgct
     6541 tctacgatat tacgtggatt ctacgacaca tgaaattata ctttctttct tagggaaagt
     6601 acaaatggaa gtgattagtg cactgttgca agaaaagtat catgtggaga tagaactaaa
     6661 agagcctaca gtcatttata tggagagacc gttaaaaaat gcagaatata ccattcacat
     6721 cgaagtgccg ccaaatcctt tctgggcttc cattggttta tctgtatcac cgcttccgtt
     6781 gggaagtgga atgcagtatg agagctcggt ttctcttgga tacttaaatc aatcatttca
     6841 aaatgcagtt atggaaggga tacgctatgg ttgtgaacaa ggattgtatg gttggaatgt
     6901 gacggactgt aaaatctgtt ttaagtatgg cttatactat agccctgtta gtaccccagc
     6961 agattttcgg atgcttgctc ctattgtatt ggaacaagtc ttaaaaaaag ctggaacaga
     7021 attgttagag ccatatctta gttttaaaat ttatgcgcca caggaatatc tttcacgagc
     7081 atacaacgat gctcctaaat attgtgcgaa catcgtagac actcaattga aaaataatga
     7141 ggtcattctt agtggagaaa tccctgctcg gtgtattcaa gaatatcgta gtgatttaac
     7201 tttctttaca aatggacgta gtgtttgttt aacagagtta aaagggtacc atgttactac
     7261 cggtgaacct gtttgccagc cccgtcgtcc aaatagtcgg atagataaag tacgatatat
     7321 gttcaataaa ataacttagt gtattttatg ttgttatata aatatggttt cttgttaaat
     7381 aagatgaaat attttttaat aaagatttga attaaagtgt aaaggaggag atagttatta
     7441 taaactacaa gtggatattg tgtcctgtat gtggaaataa aacacgatta aagataaggg
     7501 aagatactga attaaaaaaa ttccccctct attgtccgaa atgcagacaa gaaaatttaa
     7561 ttgaaataaa gcagttcaaa gtaactgtga ttacagagcc agacgcaaag acgcagagcc
     7621 gataaaatga gattaataca atctcatttt atcggctctt tccgttatgt atggattctt
     7681 ttaattagtc ttcgatgttt cttgcttcgt tgataccgct ggctaaagat tccattatgg
     7741 atagttcttt gtctgtaaag ctatccatgt atttctctat ctgtaatcgt cgggtgcttt
     7801 ttaccaagtt attagcaggt aagaaaaatt catcaacgga aacatgaagt aacgatacaa
     7861 ggtcataaag aacttgtatg ctggggtgtt gccctttatt ttcaatatta gttaagtacc
     7921 gtgggtcaat ttcaatcaat gctcccactt gttcacgagt taaacctcgt ttcaatcgag
     7981 cttctttaat ggctaaacca aaggctctaa aatcatattt atcttctttt ttacgcatag
     8041 tagaccacct ctatacattt tattgttcct actgaattaa aaacaggtat agaaaaacgt
     8101 gttatatggt ttataggttt atatttaata aaaagcacta ctaaacgcca ataaaaaaaa
     8161 ccgttatatg gtagtgctat ttacgctgtt aaaatattgt atattacttc caaatggcgg
     8221 tttgttggag gtcaacgtcg ccatgaagta catcatatac aataaatttc cttacattgg
     8281 gttcttgtca aaaaaagtcg tctatctgca atagataagt acgtccacca atgtggtttt
     8341 ataaatcata tagatagaat aacagaagca tgtaaacaga gaaataaatc tgtttatatg
     8401 cttttttggc tattcagaac ttttttacaa agtttattta tcagtaatgc aacaaatccc
     8461 cctttcacat tgggactaag agtgaaagga gataaacgag caaggctcac ttcctttcct
     8521 agacagaaag ggggtgagaa acatgaaacc atcttctttt cagaccacaa tagaaaatca
     8581 gtttgactat atctgtaaac gtgctatgga agacgagcga aagaattata tgctttatct
     8641 ttcaaggatt gcaaagcgtg aggtgtcctt ttcggatgtt ggcgattatc ttgttagcca
     8701 gtttgcgaca acagataact attcaactga ctttcagatt tttacactca atgggttatc
     8761 agtaggcgtt gaaaatgatt tgttgagtga agcattacgt gagttgccag acaagaaacg
     8821 tgaaattcta ctgctgtttt actttatgga catgagcgat tcagaaattg cagacctgtt
     8881 gaaattgaac cgttctactg tctatcggca tagaaccagt ggactagcct taattaaaaa
     8941 gtttatggag gaatttgaag aatgaaaaca caatatccta tgattccctt tcctctcatt
     9001 gtaaaggcaa cagatggcga taccgaagcg attaaccaga ttctacatca ttacagaggg
     9061 tacataacga agcgttccct acgacttatg aaagatgaat atggcaatca aagtatggtc
     9121 gttgatgaag tcttacgtgg aagaatggaa accagactga ttacaaagat tttgtcattt
     9181 gaaattaagt aatatcctct ctcctttcgt ggaagcgtgc taaaccattc cacgcttccc
     9241 gaacagggag gtttgttatt ccaccaaagc atattgagct ttcaatgtgt tttgataggc
     9301 taacgagcca ttgttctttg aaaactgaat aaaagtaatc gaatacgttt cgataagaaa
     9361 agagccaacg gaactaaccg ccatgaccta tcttataaag atagcgagcg attcatgtta
     9421 gtgatccgag aagcaatctt tagcaggatt gcctgcaacg acattcttat cgtgataatg
     9481 atactcccat acagtcaata gtccgagcgt gataaaaccg tcgcaggcaa tgagtatggc
     9541 tacatgagaa ccatgcaggg gtggaactcc cgtgagcttt gctaaagctg ttcgattgct
     9601 ggtaaaacaa cttttatgaa atccaaataa gtgatttgga aaggaggatt ttatgaagca
     9661 gactgacatt cctatttggg aacgttatac cctaaccatt gaagaagcgt caaaatattt
     9721 tcgtattggc gaaaacaagc tacgacgctt ggcagaggaa aataaaaatg caaattggct
     9781 gattatgaat ggcaatcgta ttcagattaa acgaaaacaa tttgaaaaaa ttatagatac
     9841 attggacgca atctagcgtc gccaaagggt cttgtatatg ataaaatagt attaagtcgt
     9901 atcaaggctc tttccataaa ggaaaggagc aaatgccatg tcagaaaaaa gacgtgacaa
     9961 tagaggtcga atcttaaaga ctggagagag ccaacgaaaa gacggaagat acttatacaa
    10021 atatatagat tcatttggag aaccgcaatt tgtttactcg tggaaacttg tggctacaga
    10081 ccgagtacca gcaggaaagc gtgattgtat ctcacttaga gagaaaatcg cagagttaca
    10141 gaaagacatt catgatggta ttgatgttgt aggaaagaaa atgacactct gccagcttta
    10201 cgcaaaacag aacgctcaaa gaccaaaggt tagaaaaaac actgaaactg gacgcaaata
    10261 tcttatggat attttgaaga aagacaagtt aggtgtaaga agcattgaca gtattaagcc
    10321 atcagacgct aaagaatggg ctattagaat gagtgaaaat ggttatgctt atcaaaccat
    10381 caataactac aaacgttctt taaaggcttc attctatatt gctatacaag atgattgtgt
    10441 tcggaagaat ccatttgact ttcaactgaa agcagttctt gatgatgata ctgtccctaa
    10501 gaccgtacta acagaagaac aggaagaaaa actgttagcc tttgcaaaag ctgataaaac
    10561 ctacagcaaa aattatgatg aaattctgat actcttaaaa acaggtcttc gtatttcaga
    10621 gtttggtggt ttgacacttc cagatttaga ttttgagaat cgtcttgtca atatagacca
    10681 tcagctattg agagatactg aaattgggta ctacattgaa acaccaaaga ccaaaagtgg
    10741 cgaacgtcaa gttcctatgg ttgaagaagc ctatcaagca tttaagcgag tgttagcgaa
    10801 tcgaaagaat gataagcgtg ttgagattga tggatatagt gatttcctct ttcttaatag
    10861 aaagaactat ccaaaagtgg caagtgatta caacggcatg atgaaaggtc ttgttaagaa
    10921 atacaataag tataacgagg ataaattgcc acacatcact ccacatagtt tgcgacatac
    10981 attctgtacc aactatgcaa atgcaggaat gaatccaaag gcattacagt acattatggg
    11041 acatgctaat atagccatga cgctgaacta ttacgcacat gcaacattcg attctgcaat
    11101 ggcagaaatg aaacgcttga ataaagagaa gcaacaggag cgtcttgttg cttagtagta
    11161 caaatgaatt tactacttat ttaccacttc tgacagctaa gacatgagga aatatgcaaa
    11221 gaaacgtgaa gtatcttcct acagtaaaaa tactcgaaag cacatagaat aaggctttac
    11281 gagcatttaa gaaaatataa aaagataatt agaaatttat actttgtttt atgtaaaaaa
    11341 atggtatgat taatcaagta agaagtgatt ggaagaaggc aagcaaatga atagtatgga
    11401 aaaagtcaac cagtttagag atgaacgtaa ttggcgacaa tttcataacg aaaaagattt
    11461 ggctatctct atttctttgg aagcatctga attgttagaa ctttttcaat ggaaaacgcc
    11521 ggaagaagta aaagaaacac aattagaacg aatcaaagaa gaactggcag atgtattgat
    11581 ctattcatac atgatggcag acaaccttca cctggatcta gatgagatca ttgaagaaaa
    11641 attgatcaaa aataatttga aatatcctgt agaaaaaagc gcaggtgtac gaaaaaaata
    11701 cacagagttg tagaaatgtg tcattcggct gttggaaaga gcaaaagaaa aacagtaaaa
    11761 tgagtctgga gggagagaaa atgaaaaaaa aaggaaaagc aaaaagatgg ctagtcaata
    11821 tcctcttgtt cctcttgtta ttagtaggac tagctttgat tttcaatgaa cagatcaagg
    11881 attattttgt cagagagaca ggagataaat atgctatagc gaatgtaaca aaagaagatc
    11941 tgaaaaagaa taacgataaa gatgtcagtt ttgactttga tgcggtagaa ccaatgacga
    12001 cagaaggggt catgaggtcg caaatgagag gaacagatct accagtgatt gctagtatcg
    12061 cagtcccttc agtttcaatc aatttaccgg ttttcaaagg cttggataat acgtcactac
    12121 tttacggagc aggaacatta tctccagatc aagaaatggg aaaaggaaat tatgccttag
    12181 caagccatcg tgcaacgaac cctgaattgc tgtttacacc gctagaaaat ttagagatgg
    12241 gtgccaagat ctatttgaca gatttagaaa atgtgtatac atacaaaaca ttttttaaag
    12301 aaaaagttgc tcctacagat acccaattac tgaatgaagt cgagggaaaa gaaattgtca
    12361 cactaattac ttgtggcgac atggacgccg tcacgcgact agttgtacaa ggagaactgg
    12421 aaagtgttac atcgataaaa gatgcgacag atgatatgag atctgctttc aatctagaga
    12481 caaaaacatt ttaggatact gggacataag tcgttacaga ttaaaaacag ccaaaggata
    12541 tgaatcgata aggaaaaacc aatttgctga aaaaaagaga aacgcaaaga ggtgcatctc
    12601 tgatttggtt gttttctaga tagacttgtg ttccagtttt tttatttttt atatgaaaag
    12661 gcttgcataa ataaaaattc gatggtatga tgattttgaa taagaaatgg attgttactg
    12721 tacggcaggc aaaaccagaa ttcagctaag aaacagtagg cgaattctag gtttttttat
    12781 ttttcttgag agggcgaagc aaaatgaaaa ttgataaaat tttgaataac aatgtcgtca
    12841 tttcgaaaaa tggattcggg gaagaagtcg tttgtatggg aaaaggacta gcttttcaaa
    12901 aaaagattgg ggacgagatt tcgccagaag cggtccaaaa agaatttgtt ttaagagatt
    12961 cttttgctaa aaaccaattc cagcagctga tggctgatat tccggcagag gagatcgaat
    13021 gggtcaaaca agtagtcgaa ctggctgaag ataaactaaa agttgaattt tcgcctaata
    13081 tttatctgac attgaccgat catcttcatt atgcgatcac acgagcaaaa gaaaatatcc
    13141 aactgcctaa tccattatta tttgaaacaa aaaaatttta tccgaaagaa tatcaagcag
    13201 cgaaagaggc tcttttgttc gtgaaacaaa agacgggtgt ggaactttct gaggatgaag
    13261 ccggttttat tgcctttcat tttgtgaata gccaacaagg aaacgaagac atgcaagtga
    13321 cgatgaccgc taccacaatg gtaagagata tactgagtat catcagtaaa ttcttcggca
    13381 tcgtgttcga tgaagaatct ttgaattatc agcgtatcat tactcatctg cagttttttg
    13441 cccagcgtta tctcaaaggg gaaggatctg acgagcaaga tgaatttctc tacgcattag
    13501 ttcaaggaaa gtatccaaaa gcgtttcatt gtgtcgaacg gatcaatgag tatcttcgac
    13561 aaacgcaaaa acaggaaatg gggatcgcag agcagattta tttaacgatt catattcaaa
    13621 gagtagtaag tgagaaaaaa agcatccaat cataaggatt gttactgtat ggcaggcaaa
    13681 acctggattg aaaaaatagg ttcacgaatg tttgtgactg tttttttcaa tctgggtttt
    13741 tattttttaa ataaaaaaga aagaaggaaa aaacaatgga taatcaagca gttggtcgtc
    13801 gtgtatggga agcggtagga ggacaaaaga atgtcaaaag tttagtacat tgtgctacgc
    13861 ggttgcgatt cagattaaaa gatgaatctt tagcagatac gcaaaagtta aaagaagacc
    13921 cggacgtgat ccaagtcgta caaagtggcg gtcagtatca agtagtgatt ggcagtaatg
    13981 tagcagatgt ttatcaatcg atcgtggatg aggaaggtct tgcagtttcg gaggatggaa
    14041 aagaagagtc caaaaatatt ttgaatcgtc tgatcgatat tatctctagt atatttacac
    14101 cttttctagg ggcgatggca gccgccgggg tattgaaagg attcttatca ttggcaacgg
    14161 tgatgggctg gctgacagct gatagcggta cttatcaaat attgtttgct gcagcagatg
    14221 gtgtctttac cttcttacct gtcatgcttg cttttacagc agctaaaaag ttcaaagcca
    14281 accagttttt agcagtagca attgctatgg cacttgtgta tccagctatc acagcagttg
    14341 ctggtgcggg agaagctatc agtttctttg ggatcccagt gattctttca ccatctggtt
    14401 atacttcttc tgtcatacca atcattctag ccgtatgggt tcaaagtaaa ttagagccat
    14461 tcgtaaaaaa agtgattccg caatttctgc aaatgatttt agttccgctt gtcgttttag
    14521 tagtgatggt tccattaaca tttcttgctt taggaccaat cgggacggtt gccggtaatg
    14581 ctttaggcgg tctcttcaac tcgatttatg gatttagtcc aatcgtagca ggtctaatca
    14641 tgggaagttt atggcaagta ttcgtgatgt tcggcatgca ttggggattc gtaccgatca
    14701 tgtttttgaa tatcgaacaa tatgggtttg atgtattgat gccaatgctt cttccagcca
    14761 tcttggcaca aggtggggct gctttagctg tagcattgcg aacaaaagat acgaaacttc
    14821 gagcattagg gatttcttct acagtgactt cgttatttgg aatcactgaa ccaactgtgt
    14881 atggtgtgac attacctttg aagaaaccat tcattgccgc ttgtatttct ggaggtattg
    14941 gtggagctat tatcggattt tccggagtaa aagctttctc cagcagtttg gtcagcttat
    15001 tgacgattcc gactttcatt aatacggtgg atggtgtaga gtcaaatgtg acagtagcag
    15061 taatagctac aggaattgct tttgttcttg cattcgtagg tacattgatt ttaggatttg
    15121 acgaacaaac acaagacaat caattagaaa acaaacatgc gaatgcagga gaaccgatta
    15181 cttcagcacg gcatacctta aaaagtcctt taacgggtaa agtactgcca ttgagtgaag
    15241 taccagacca agttttttct tctggcgtga tgggaaaagg tatcgctatt gatccagaag
    15301 taggagaact agttgcacct gcagacggag agatcacgac tatttttcca actggacatg
    15361 ctgtagggat cacaacgact gacggagcgg agattttgat tcatatcggg atggatacag
    15421 tagaattaaa tgggaatggc tttgagatct tagtcaaaca aggggatctc gtcaaagcag
    15481 gagatttatt gattcgtttt gacatcgaag caattagagc agctggctat agcgtaatca
    15541 ctccagttgt cattacgaat actgatgcat ttgccgatat acttgaatta gaccaaaaag
    15601 agattattgc aaacgaagat gttctggcaa ttgtaaaata aggaggaaat caggatgcaa
    15661 tcacgttttt cagaaaaatt tttatggggt ggagcaactg ctgccaaatc aattagaagg
    15721 ggcttatgat cgagacggaa aaggactatc cgttgcagat gctatgcctg gaggtaagca
    15781 gcgctttgcg attatcggca gtgaagaatt tgactggacg attgatcaag aaaaatatat
    15841 ttacccgaat catcgaggaa tcgatcatta cgatcgtttc aaagaagatc ttgccttgtt
    15901 tgcggaaatg ggattcaaat gttatcgttt ttcgattgcc tggacacgaa tttttccaaa
    15961 tggagacgaa ggtactccta acgaagcggg attagaattt tatgatcagc tgattgatga
    16021 gtgcttaaaa tatgatattg aacctgtgat cacgatttct cattacgaaa tgcctcttca
    16081 tttggcaaaa gaatacggtg gctggaaaaa tcgaaaactg atcgacttct atgaacgatt
    16141 tgctcagaca gtattagaga gatacagcag taaagtcaaa tattggatga ctttcaatga
    16201 aatcaattcg gcctttcact ttccagcttt aagtcaagga ttagtaaaaa gcaatggagc
    16261 aggagagtac caaaatattt tccaagcatg gcataatcaa ttcgttgcaa gtagcaaagc
    16321 cgtaaaaatc ggccacgaat tgcgttcgga tatccaaatc ggctgcatga ttatctatgc
    16381 aacaacttac agcatcgatg ccaatccagt caatcaggca gcaacaatga tccaaaacca
    16441 agaattcaat tttttctgca cggatgtcca agtacgtgga gaataccctg catacacagc
    16501 gcgtacccat aaaaaatatg gagtcgatcc ggaaaaatta gagcaaacgg aaaaagactt
    16561 ccagttgcta aaagaatatc cagtggacta catcggattt agttattata tgtctacggc
    16621 aatcaatgag actgatccac aagcagcaac ttcagaaggg aacttgctgg gaggagtaaa
    16681 aaatccgttt cttgaggcaa gtgaatgggg ctggcagatc gatccagaag ggctgagaat
    16741 cgcattgaat gaattataca atcgttacca gaaaccatta ttcattgttg aaaacggtct
    16801 tggagccatt gatcatatgg aagaggatgg cacgattatt gatgattatc ggatcgatta
    16861 tttacgtcgt catatcgaag cgatggctaa tgcagtagca gacggtgtag atttgatggg
    16921 ctatacgcct tggggatgta tcgatcttgt cagtgcatca acaggagaaa tgagtaaacg
    16981 ttacggcttt atctatgtgg atctagacga tgaaggaaat gggacactga atcgatcaaa
    17041 gaaaaaatct tttgattggt ataaagaagt aattgcctca aatggcgtaa acttgtaatt
    17101 aaagcagctg aatgaaaaag aacacacttc tcacatgtga tttgtgttct ttttttattt
    17161 tttatgtgct attcttttag taatagtaaa gcaaagggtg acgttatgaa aaggacacgt
    17221 gtactatact tggaagtcgc agataaaatt aaagaagata ttttctcagg aaaatatcca
    17281 gtcggttcga tgttgccgac agaaagtgaa ttagaagaac tgtttcaagt cagcaaaatc
    17341 actgtccgaa aggcaatcga actgctggca tcagatgaat atgtcgcaaa gaaaagtggt
    17401 cgtgggacga cggttctaag caatcgtcct tacaatcgat tatccaaagc ggcaagcttt
    17461 acgcaaatct tggagaactc ccgatatgaa gtccggaaag aaacgctgga tttgcaattg
    17521 gtccatataa agaaggaaga tcctttgttt tcctttttcg gtgagcaagc tgtttgtttt
    17581 aagcgcttat attatttgaa caatcagcct tatatttatt ttgttcatta tcttccacaa
    17641 gaaatgatgc aattcagcag agaagaattt ggcaaagaat ctttgtatcg tttgctgacg
    17701 aaaaaaggat atactattga ctcattttct gatgatttct cggcagtatt tttaacagaa
    17761 gaagaacaag gaattctgaa aacagacgca cagatcggca tcaaacggat acggaaatcg
    17821 tctacgcaaa ttggggaaat gattgaatat tcggaggcgg tctaccatac ggagctgcat
    17881 ccttaccata ttgattatga aacataaata aaaaaagagg ttgtgaaaaa gctgccctgc
    17941 atcaagtaat aagaacagcc aaacaaaaat agcttctcat atttttcgtt tggttaggct
    18001 tattaccgca gatacacggc tgtttctgga ttaccattta ttctggataa gggggtgtgg
    18061 cacgattttc agaaggaatc tatgaaccaa tcactgtatt gattttagct tcgatggtaa
    18121 ccccattcgg cttgacgatc gcttatttct tagggaaaat cattcgtaaa aatatactca
    18181 atcgtcaaga aatcgataca ttgaagaccg cttttcctat ggggatctgt cagatcacag
    18241 agggatgttt ccctatcgta ttgaacgact tggcacgcaa tgtcatagca acgggagtcg
    18301 gtgggacagt aggtggcgga ttaagcatgt tttggggcgc agatagccgt attccggcat
    18361 cgggaatgtt tgcagtcgct acgatgacca gaccatgggc attcattggt gcattgttag
    18421 ctggttcttt tgtcacagga ataaccattt tattattgaa aaaaccagta gatccgaatg
    18481 cagaaatcat ccaagaagaa aaagaggaag aagacatatc ttgggatgat ttgacaatta
    18541 gttaggagag gcagaatgga ttacaagaaa aaagtacaat agatgtacct tgagacagga
    18601 atggtatgaa agaatcagag ttagaaagaa tcgaatatac agattttggt ttaggagcga
    18661 tcgaaactgt ccagagcatc ggcaccctcc ttgaaatgac gatgaaggaa aaatgagaac
    18721 ctttcgttgt cgaaagggtc tggtatacct tatgtcgaag gaaaggaaac aaaaaaactt
    18781 tcctgtacta ttccaaaaag gaaagaaagt tattatacag taaaaaaaga aattattctt
    18841 catgctagag atcagtacac gttcgagccc aatatcaaac actagttcca ggcaggcgca
    18901 acaggtgcgg ttatcactga attttcttcc agcagtgacg attcaagtga tatttttaca
    18961 aatccagcta tttatcgtta acaaaaagat ttttaggaga agctgatgaa caaaaatgat
    19021 caaattttga tcgtttagtt cgtcggcttt tttatttaga gaaacatcat ttatcagtga
    19081 aacataacac gagaacaaaa tgattttttc gttaataaaa gaaacaagta tttgttgttt
    19141 aaaataatac acgttattgg catataggac gaaatcccag tatcttctat aagtaagtca
    19201 tttcatatcg atgaaaacac gttatttatt tttgttttat ctataaaatg gctgaaaaat
    19261 gatttttttc taaatactat atatagtggc gtctatccta tataccacta aaaaactaca
    19321 aaatctagtc ttttatttta aaaaaacacg tgtagaatga agatagactt ttgagttagg
    19381 gagtgttttt ttatgaaact tactgttttt tgcggttccc gttttggtaa taaagaaagt
    19441 tacaaattta ttgcgcaaac gttaggaaaa tacatggcac aagaagaaat cgaactggtt
    19501 tacggtggtt catactccgg gattatggga gtgattagta aaacagtact ggaaaataac
    19561 ggaaaagtca caggtatcta tccgaatggg ctttttgaaa ctgaattgcc tggaaaagat
    19621 gttactacat ttattcctac agaaacgatc gatgaaagaa aggtattatt atttgaaaag
    19681 ggagatgctg tcctcgtttt ccctggaggt ctaggtacat tggaggaatt ctcacaactt
    19741 ctttcttgga tagcaatcgg tctgacgccc gacaagccaa tcggaatatt gaatattggc
    19801 ggttattaca gcggcttgca acaattatta gaaacttttg caaaagagga ttttatggat
    19861 aaaaaatggt tggatcaagt aatcttttcc aacaatccat tggaactggt caatgaatta
    19921 cgagcagtat ctattaaaaa tcagttatta ttgaaggagg caaaataatg agtcaaatat
    19981 atttagcagg accatttttt tcagaggagc aaatcgatcg agtaagtcgt atcgaaaaag
    20041 cgttggagga aaacaaaaca gtcacaagtt tctactctcc acgtcatcac caagaaagca
    20101 attatgaact attcagcgct ggatgggcgc aggaagtata cgaaaaagat atggaagaac
    20161 tgacaaatgc agaatttgtt gttgctatcc ttgactttga gcatcaaaca atcgatccag
    20221 gaacggctta cgaattaggt gtggcaacta tgttgaaaaa accgatgatc attgtacaag
    20281 aagagacggt acctacgaac ttgatgatta cacaaagtct ccatacgtat ttaaaatcag
    20341 accaagctgt acgtgaatat gattttgaaa cattgcctgt tgaaacttac gtaggcgagt
    20401 acttatagaa gaggaagggt tgtatgtcag tgattttgat ttcaggaacg attggtgcag
    20461 gtaagagcag tttgacggat atgcttgcaa aagagattga ttctaaacca ttttatgaaa
    20521 atgtcgaaga taacgaagta ttgccgctct tttattcaaa tcctgagcag tacgcttttt
    20581 tattgcaaat cttctttttg aacaagcgat ttttagcaat gaaaaatgct ttagtgaacg
    20641 atgacaatgt gctagaccga tccatctatg aagatagcct gttgttccat ctaaacgctg
    20701 atttaggtcg ggtaacggat atcgaagtac agcagtacga tagcttatta gatacgatgc
    20761 taaacgagtt agatgatgtt gctccaaaaa aacggcctga tttgatggtc catatcaaag
    20821 tttctttgga cacgatgcta gaacgtatca aaaaacgtgg gcgtgactat gaacagctag
    20881 aaagcgatga gacgttatat acctattatg aaacgttgaa cacccgctat aatcaatggt
    20941 atgaagattt tgatgtatgt cctaaaatac aagtggatgg ggataaattc gattttgtag
    21001 aaaatccaga agatgcaaaa aaggtgatcc gtcagatcca agaaaaatta gctgaattgg
    21061 aaggaaagcc tgctggaaaa tattttacgg ataaccagca tgcaaaaggt caagaattag
    21121 gctaaaaata aaaaaccaaa cgacgggaaa gaaaaattac tacgtgatgt agaataagcc
    21181 tttctatcgt ttggttattt tatattcaaa tatttgaaat aaataaatct ttactctttg
    21241 atttgtaaac tgaaatgatc cactgtttgt tttactgatg aagcttgatc aaaggaagaa
    21301 gctgagtttg aagttcataa aaatcagcca tagtgagttt tgcttggatt tgtttatctt
    21361 cttctacttt ttacataatt ttgaacagga taagattcaa atgttttaaa tgaatctttg
    21421 actagtttgt gacagttgtc gcatatgctc aagcttcctt ggtcattaaa ttctgtatgg
    21481 atatttttgt tgttattaca tgtacaataa tcaggactat cgatcatcca tttcgtctcc
    21541 tttacaatca tcttgcttct ttactatagc agaaaagaag gggagaaaca aaagaagatc
    21601 caacagacgg cactatagac tgtagatgtt gccatcctca tccttcaaac ggtaattacc
    21661 gatttttgta aatatgactg ttgtttcttt tttattttct tttcctgaaa taatatattc
    21721 gtcatctttg gtagtgatct tgatatctgt cagcactgtt ttttcttcag atgattcaga
    21781 agaatcagtt ccttgtgtct gatacgtgcg tgtccattcc atttttttat ctgtaaatgt
    21841 caactgtccc gcggataaat ccatatcttt acgtactttt agcgtatata cactgttggt
    21901 ggtatctgct ttttccgctt ttaagacaga agaatcggtt gtttcgtccg aggattggtc
    21961 agagccgcca aacaaatcac agccagtcaa tgtcaaaagt cccgcagaca aaataaatag
    22021 tacgattaat ttcttcaaaa tacttccccc atttgatagg ataaagaaat tataccattg
    22081 gaatgtaaaa cttcaaacgg atggaagaga aaaacgcaca aaacacccac aaatagagta
    22141 aattttagaa taaaatttta aaaaggcaaa aaatccgcca ctttcagaaa gcgttttaaa
    22201 aaagtgttgc aatcttttaa aaaaatgcta tattaaaaat gaaccggttc aaatacttgt
    22261 ttaagtataa ttaatagtga gaggaagttg ctctactgaa gactttagtt atatggatat
    22321 tgatgacaag caacactatg caaagaatgc ggtcatcata atcgaacaac aagaaagaga
    22381 aatcaaatgt aactggcagg agtgagaatg tatggcatcg atccgtgatg tggctaagat
    22441 ggctggtgtt tcagtgggaa cagtttcccg ttacttgaat ggtcagcaac tgaaggaaaa
    22501 gaacatgaaa aaaatagcag aagcgatttc agcgttggac tataaagaaa acatcattgc
    22561 taaagggttg aagaataatc gaagcttctc tattggactt ttgataaacg ggatttccag
    22621 ccgttttggg tcggaagttg tctcagggat cgaaagagta gctgaagaaa atgggtatag
    22681 cttattatta agcgggtttg ccgatcagcc agaaaaaatt gaacaaaaaa ttgaatacct
    22741 gatgaagcat gtgatcgatg ggctgattgt cttcttatcc gaagaagagt ggtctggatt
    22801 tgaaatgctc acaaaaatga gtattccagt tatcgcattg aattgtccag aaggaccagc
    22861 aggtattgat acaatattag tgaatgatcg agaaagtgta gcaaaagtga tcgctcatca
    22921 tggtgaacaa ggacataaaa aaatcggatt tattgctgcc actcaaacag actacgtagc
    22981 aagagaacgt ttagcaggag ccaaagaagc ggtccaaaat gatccatcga tccaattaga
    23041 ggtctttaca ggagactatt ctagaaaaag cggctattac ggggcaaaag cattattaga
    23101 aaaaggggca actgctattt ttgtcagcaa ttacaatatg tcgattggtg ccatcgaatg
    23161 tttcaatcaa gaaggtgtca gaatcgggaa ggatattgcg ttcagtcact atgattattt
    23221 agataaaaac gatctgagta tccttccgaa aatcacgatc acgccaccaa cagataaaat
    23281 cggggaacga gcagcagaac agctgttaaa acgaatcaaa aaagaagagg aagaaaatga
    23341 aaaagtgatt gtgatggaaa atatcataca tggaatcaac taaaacgaac taaaaaagaa
    23401 aaagaggtgc tttgatgaaa aaatatcaat ttccagcaaa tttttggtgg ggttctgcag
    23461 caagtggccc acaaacagaa ggaaggattt caggcgatgg aaaaggagag aacatttttg
    23521 attattggta caaaaaagaa ccgcaaaagt tctttgatca agtaggacct gaaaaaacct
    23581 cgcaagttta tacgaagtat caagaggatg tacagttgat gaaacagact gggcataatt
    23641 catttcgaac atctatccaa tggagccgtt tgatacctga tgaatctgga acaatcaacg
    23701 aagaagccgt tcgtttctat catcgttatt ttgatgagtt gattgaaaac ggtatcgaac
    23761 cctttgtgaa tctgtatcat ttcgatatgc cgatgtatct gcaagaaaaa ggcggatggt
    23821 tgaaccgaga aacggttgaa gcctatgaaa aatatgccaa aagttgtttt gaattgtttg
    23881 gttcgaaagt aaaaaaatgg tttactcata atgaaccaat cgttccagtc gaagcaggtt
    23941 atttatatcg atggcattat ccagaagagt cagatatgaa aaaagcgatc caagttggct
    24001 atcatgaagc gttggcttca gcgttagcca tcaaatctta ccatgaaatg gagcaaggtg
    24061 gtgttattgg tatcatcttg aatctgacac caagctatcc aagagacgaa tcaaaccctg
    24121 aagatgttaa agcagcgcaa atagcagatg cgttcttcaa tcgttcattc cttgatccag
    24181 ctgtaaaagg cgagtttcca agtgaattga ttgagatagt aaaagaactt gatctcatgc
    24241 ctgtgatcca tgcagaggat ttgacggtta tcaaagaaaa tacagtagat cttttaggta
    24301 tcaattatta ccagccacgc cgggtaaaag caaaagaaac accaatcgat actaaaaacg
    24361 gtccaatgcc tgaggattat ttcgacaatt atgaaatgcc tggtcgtaaa atgaatcctt
    24421 atcgcggatg ggagatctat gaacaaggaa tctatgatat tttgatcaat gtacgagatc
    24481 attatggaaa tatcgactgc tttatttctg aaaacggtat gggtgtagaa aacgaagcac
    24541 gatttatcaa agaagatggc atgatccacg atgattatcg gatcgaattt gtccaatctc
    24601 atctgaaata tgtccatcaa gccattcaag aaggtgccaa ttgtttaggc tatcatatgt
    24661 ggacatgtat ggataactgg tcgtggacca atgcatataa gaatcgatat ggctttattt
    24721 cagtagactt agcacaagat ggaaaaagaa caatcaaaaa atcgggtcat tggttcaaag
    24781 acgtatcaga taaaaacgga tttgaggctt aagaaagaat agttgagagc aaatgcgtga
    24841 aattctccat tcttaccatt ttgtgaaaaa gaagttcaat tatttacgat ctttttcaac
    24901 ttttttgtct ttctatttca ttttttgtat ataatagaat agtcaaaatt aaataggagt
    24961 ggaggaaagc atgaggaaaa aaagaccgtt tcttctcttt tggttattcg gttttttctt
    25021 actgcttatt ttatccgcag cattttcagg aaaaatgaca gcagatcttg ctcagaaaga
    25081 aacagaaaaa atcgtagcaa gcaaagaatc agtagataat ttcaaaaaag aattctcaac
    25141 ttattggtgg gcggagtata aaataactgc caattacgaa aaaggcgaat tggacattta
    25201 tttgccaaaa ggaaaagaag aaaatcaatt tattaatatc gatgacgtac ttagtgcgat
    25261 ttctctcaga caatatcaat ttaaagacaa gcatttgacc gttttgcttt tgactcatga
    25321 tggcgacaaa gtagcccgtt tgaaaaatgg aaaagtgatc accgcttatt ctgaaaaagt
    25381 agacaaaaaa gtggtcgccg atcgtttagc ggaatggaaa aagacaaatg aacaagtcaa
    25441 gaaaacaact gtctcttctc aaaaaaacga gtcgacagga acaagtcctt cagattctgg
    25501 cagtggacaa acgataacgg gaagtgaaga aaatcaagcc tcaattatta cgaatgagtc
    25561 atcacaagga caatatcaat cctatggaca atgaaaaaca gaggttgtga aatcagcgtt
    25621 ttgacctgag tattagagaa acaaacggca aaatagttcc tcatattttg ccg
//
LOCUS       DACDYI010000047        24860 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.047, whole genome shotgun sequence.
ACCESSION   DACDYI010000047 DACDYI010000000
VERSION     DACDYI010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.047"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(125..895)
                     /locus_tag="IWM52_001775"
     CDS             complement(125..895)
                     /locus_tag="IWM52_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9974023.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(888..1823)
                     /locus_tag="IWM52_001776"
     CDS             complement(888..1823)
                     /locus_tag="IWM52_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9974024.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(2139..3788)
                     /locus_tag="IWM52_001777"
     CDS             complement(2139..3788)
                     /locus_tag="IWM52_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP9974025.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(3905..6508)
                     /locus_tag="IWM52_001778"
     CDS             complement(3905..6508)
                     /locus_tag="IWM52_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP9974026.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(6520..7569)
                     /locus_tag="IWM52_001779"
     CDS             complement(6520..7569)
                     /locus_tag="IWM52_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAP9974027.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(7595..7909)
                     /locus_tag="IWM52_001780"
     CDS             complement(7595..7909)
                     /locus_tag="IWM52_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAP9974028.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(7925..8371)
                     /locus_tag="IWM52_001781"
     CDS             complement(7925..8371)
                     /locus_tag="IWM52_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP9974029.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(8372..10348)
                     /locus_tag="IWM52_001782"
     CDS             complement(8372..10348)
                     /locus_tag="IWM52_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAP9974030.1"
                     /translation="MKIEIIIVHLSCIQEVKIMGIEFSERQMKILYFLIENEHTTYKE
                     LSEQLYVSTKTISKDILILKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKIN
                     IADSPEDRLTYVFAKLLNTNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLV
                     SDRNGVRIKADEKSKRRLMSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDI
                     LKKVAELLNRYMNKTQLKFTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNT
                     LILIKMIEKAFSIIVPIDEQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVR
                     IKLNEFYPDEELINNLVLHLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEE
                     IEERFCIHFNEDEIAYVTLHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFA
                     DMVEIEDVLSINELQDRKVTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQL
                     NEQTEDAFLNLLDKRFCFFTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKL
                     SSTAMNDFAMPHIEVDFIKKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKI
                     YETFNEIASNHELLANLKTSSSFEKLVYILKEGL"
     gene            complement(10506..11402)
                     /locus_tag="IWM52_001783"
     CDS             complement(10506..11402)
                     /locus_tag="IWM52_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9974031.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(11395..12075)
                     /locus_tag="IWM52_001784"
     CDS             complement(11395..12075)
                     /locus_tag="IWM52_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9974032.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(12076..12858)
                     /locus_tag="IWM52_001785"
     CDS             complement(12076..12858)
                     /locus_tag="IWM52_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9974033.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(12851..13768)
                     /locus_tag="IWM52_001786"
     CDS             complement(12851..13768)
                     /locus_tag="IWM52_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9974034.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(13932..14255)
                     /locus_tag="IWM52_001787"
     CDS             complement(13932..14255)
                     /locus_tag="IWM52_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9974035.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            15043..18270
                     /gene="fss3"
                     /locus_tag="IWM52_001788"
     CDS             15043..18270
                     /gene="fss3"
                     /locus_tag="IWM52_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP9974036.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            18383..18697
                     /locus_tag="IWM52_001789"
     CDS             18383..18697
                     /locus_tag="IWM52_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9974037.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            18710..19084
                     /locus_tag="IWM52_001790"
     CDS             18710..19084
                     /locus_tag="IWM52_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9974038.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            19094..19429
                     /locus_tag="IWM52_001791"
     CDS             19094..19429
                     /locus_tag="IWM52_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP9974039.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            19512..20852
                     /locus_tag="IWM52_001792"
     CDS             19512..20852
                     /locus_tag="IWM52_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP9974040.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            20930..21604
                     /locus_tag="IWM52_001793"
     CDS             20930..21604
                     /locus_tag="IWM52_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9974041.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            21837..22271
                     /gene="dcm"
                     /locus_tag="IWM52_001794"
     CDS             21837..22271
                     /gene="dcm"
                     /locus_tag="IWM52_001794"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP9974042.1"
                     /translation="MRFLDLFAGIGGFRLGMEQASHHCIGFCEIDKFARRSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQRFSDTRGTLFFEIARF
                     TTLLRPKLLFLENVRGLLNHEGGATFETILRTLDGLGYDVEW"
     gene            22272..22979
                     /locus_tag="IWM52_001795"
     CDS             22272..22979
                     /locus_tag="IWM52_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP9974043.1"
                     /translation="MLNSKAYIPQSRERIFLIGHSRDACTEQVFPIIGPSSTSDQNIR
                     NLVNINPSNRGMGGQVYGSDGVAPTLTGDEGIKIALPARDGISVAGLLPGKFEQGNRV
                     YEVTGTAPTLSTKQGGTKIMIRKNETEYQEVKPEDSVNLAFPNSTSRRGRLGKQSVHT
                     LLTGDQQAIVTDQYQIRKLTPRECWRLQGFPDWAFDRDSQVNSDSQLYKQAGNSVTVP
                     VIFDIARRLKELDVHGL"
     gene            22969..23259
                     /locus_tag="IWM52_001796"
     CDS             22969..23259
                     /locus_tag="IWM52_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974044.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            23518..24702
                     /locus_tag="IWM52_001797"
     CDS             23518..24702
                     /locus_tag="IWM52_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9974045.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            24717..24836
                     /locus_tag="IWM52_001798"
     CDS             24717..24836
                     /locus_tag="IWM52_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP9974046.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
BASE COUNT         7736 a         4788 c         4639 g         7697 t
ORIGIN      
        1 cactaaacgg tgttcaatca gctgaccctc ggtattagct caacaaacga caaaatatga
       61 ggaactattt tcccgtttgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc
      121 ttttctaact tctcaatcct cttcctttat tgatcaaata ccaagtaacg gcgtacaaaa
      181 caacgatgaa tagcacaagt acgatcatcg atgttgctac tggcacatcg attgttccaa
      241 ggaatccgta acggaaaccg gagatcatat aaacgatcgg attgactttc gaaacagctt
      301 gccaaaatgg cggcaacata gagatggcat agaaaacgcc acctagataa gttaacggct
      361 gcaagacaaa tgtaggcacg atcgatacat cgtcataaga ctgagcaaaa atcccgttga
      421 gcagtcctgc taacgaaaac aagattgccg tcatcaataa tgtaatgatg accatgaacc
      481 atgaatacac atgaagtggt acaaagaaca aggaaatgat cgttacgaga gagccgacga
      541 gaatactgcg acccactccg ccaatcacaa agccccaaat gatcacatgc gtaggaacag
      601 gcgctaccaa gatttcttcg atgtttttct ggaatttttg agagaagaag gacgaagaaa
      661 cgttggcata cgaactagtg atagcagaca tcataatcaa accaggcacg atgaattcca
      721 tatatgaaaa accacccatg tcaccgatcc gaccaccgat catttttccg aaaataacaa
      781 agtacaagga tgtcgtgatc acaggcggaa ccagtgtttg gacccagata cgcaaatagc
      841 ggttcgtttc cttgatagct aaacttttta atgcagtaaa ataaagatta aacatttttc
      901 tcctccgtat gattattttc ttctgtgatt ttcaaaaaca gttcttctaa acgattcgac
      961 ttattcctca tagaaagaac tttgattcct tgtcttgtca gctgatcgaa gattccgttc
     1021 accccttgat ttcgttcaac ttcgacagcg attgtctgtt catcttcaaa tgtacaagcg
     1081 tatccttcaa tcacaggttt ggtctcatat ggcgctaaat caaagataaa tgtttcaaat
     1141 tgcagtttgg atagaagctc tttcatacta gtattctcga tcagttcacc tgattggatg
     1201 atcccgatgt tgcgacaaag catttcagct tcttctaaat aatgcgtcgt caaaatgatc
     1261 gttgtaccat tttcgttcaa ttctcggaga aactcccaca tctcgcgtct caattcaata
     1321 tcgactcctg ctgttggttc atccaagatc agcaaacgtg gttcatgcat caatgcccta
     1381 gcaatcatca gtcttcgctt cattcccccg gaaagcatac gtgcacggac atttctcttt
     1441 tcccaaagat tggactgctt caaatatttt tcgctgcgtt tcatcgcttc tttgcgagag
     1501 acaccgtagt agcctgcttg attcacgacg atttgctgga ccgtttcaaa gggattgaaa
     1561 ttgaactctt gcgggactaa tccaatctgt tgctttgctc ggactagatc cgtatccaga
     1621 tcataaccga agacattcac ttttccagat gttttattga ctaaagaagt aatgattccg
     1681 atcgttgtag acttcccagc tccatttggt cctaatagcg catagaaatc tccttctttc
     1741 acggaaagat cgattcctcg caatgcttcg accccacttc cataaacctt tttcaaatta
     1801 tttatttcaa gcgcatttgc catttatttc ctatctcctt atagacatga ttctttattg
     1861 tatcatggaa tacagcagat gaaaaaacaa cttggttcca gcgaaaaatc aggttgctgg
     1921 gctcaggaaa atctcctaat gaaagacatc aatcggacga agtcagacaa ccttttcaga
     1981 aaaaacaaat cgaagataaa ggattactat tcatatgtgc aacaaaagga gcgtgagaca
     2041 aaagtaaaga acacttttgt ctcacgctct aaacacgtat aaacggcgga agcaaaagcc
     2101 aagatttttg atccaataaa tactttcgat cacttcgcct atcccaatac atgccactgc
     2161 cgcttttcat caaataacgc ggcttccgtt tttgcggttt ctactaaagt ctctgggtaa
     2221 tccaaaactt tcagcaaggc aatcgcattt cgggtccttg aaggtccttc ttttaatttg
     2281 aaatcaaaac tgattccttt tccttctgtt acttgttctt caaagtggac attctcacaa
     2341 taattcttta aaatctctgt caattcgata tcatgtgtgg cgacaaatgc taaagatgga
     2401 tactccgcaa gccaccgaac gactgaagcg gaagaagcaa tccgttcgat cgtatttgtc
     2461 ccttttaaaa tctcatctac gaagcagtag cagcgttctt tggaagccac ttggtccaac
     2521 aaacgtttga tcgatttgat ttctgaaaca aaataactgt cgccttcaaa taaatcatct
     2581 tcgattgcca tggaagtcaa cacatgtcca ggttgcaggg taaactgttc tgcaatcgct
     2641 gtttggatcg tctgtgctag aatacagctg atggctatac ttttgacata cgtagatttt
     2701 cctgatgcat tggaacccgt gactaatgta ttcttctgcc agttgaccgg attcactacc
     2761 gcttctttta acaatggatg atagatatct ttcgctgtca caccgccctc agtaaactct
     2821 ggtcggcaag tgatgggcat ataagtacgg taattcaata ttgcagcggc tacttctaat
     2881 tctcccagca attcccacag cttgatggct tcagttgaat gcttttctaa tttagtgaga
     2941 acgaaatgat aggagataaa tggcagcatg aacatgatgt tgacatattc aaaaatcaac
     3001 tctgcttcac tcccactctt catacgaaat gaaaaaccta atttagggat gtctcttaat
     3061 ggtttagttt gctgtttcag ctcttcttga agtggtgtcg gcagttttgc tagctgattg
     3121 gcggctgaaa tcgttgaaac caaataactc atgctattca gctctgtttc tagtgtttgt
     3181 ttcttcacta gataatagac cgtattgaat actaacccac cgattgccaa atagattcct
     3241 agtacttgtc ctaaaagaag caaacccaaa cccaccagcg gaagcaagcc aagaaaaata
     3301 aataaaccta cgcgtccaat ctttttactt tttccatccg ctagatattg ttttgcaaaa
     3361 ttatgatctt tttttcctaa acgagcgaat tgatattgga ttttttctct cagttgagga
     3421 ttttcctgat aaaaagcaat cagtttttct aactgctgat cttcaccaaa atcaaagctg
     3481 cgtaaacgtt gatacaacgc ttctgaacct acactagaat aggtactgtt gattccttcg
     3541 aatacttcga acatatccag gtcataccaa gtaagatcat cgatttcgct atcccatgaa
     3601 cggaacttct tctccgtgag ccatgcttcc ttcaagcttt cttctttatc aaatctcggt
     3661 tgataaggaa ttttttccca tttgctacga actgtttcct ttaatttcaa acgtgaaaaa
     3721 atgtctaaag caactaataa aataacagct gcaataataa ttaaaacgat aaattctctt
     3781 tgacccatat ccctctccgt tcctttattt gttagcttta gtatagcact gttgcgctat
     3841 ttgaaaagag ggtaaaaaaa cacctttcaa aataaaaggt gtttttttct tgtatattac
     3901 atatttatct ctcatttatc aaaattgaca aataatcata gggaggaatt gttgttaatt
     3961 cttgtttatt ctccagtgca ttgacaactg tcccaaggga aaggatagcc tcaggtacta
     4021 ttttttcttg ttctgttgga ttagctaagc gaagaacaaa tccttgttca tgaaaataag
     4081 aaggataaat cgatgataca attaagtctt caggtaattc aatcaatgaa aatttacgag
     4141 gaacatcaag ttcattttgt aatggccaaa tcttgttatc taacctgtga ataaatacat
     4201 tcagtttctg cttttgataa gaaatagatt gttctaaccg tttttctgcc tgttttgcca
     4261 ctttgaactc attaaatctt ccttcttcta cattaaatcc aaaagtgact ttatactcac
     4321 ctatctcttg agctagcggt gtttccatca tgacatgccc ttcatttgtc gtatctcctg
     4381 aagctcgccc aggtcgccaa gcaagattag gttttccaag ttcccctgtt gttgaaaata
     4441 aggttatgat taattgattt tctatacgtt catactcttt taaaccatct gcataaaacg
     4501 taaaataatg atcattttct gccacagaga cactcttatc gaatatttct aaattaacag
     4561 gtttctctac catagtttct gaccaattcg gaggaatgac atcaggggta ttttcaataa
     4621 atccgttttg aatttgtgca attgattttt cagatttatc taaaacatta attgctaagc
     4681 gcatacgatg cgagtaaaca gtattatgaa ttttgagtgt gccttcaacg atttgacttt
     4741 ctcccagact gaacgatatt tcataagata tttcttccat ttcgggtttt tcagataaac
     4801 gatcttttaa atctttagga agttgagctt ttccgtaaac aactaatgtt tcttgtaaag
     4861 aatctttata tactttagct gtttctaatt ttagagatag ttctgtatct ccttctaatg
     4921 gtgaataatc atacgtatcg ccatcatttg ctgaatcgat caaatgaacg aaatcaagat
     4981 attgtctatc tccaactatc aaattcaatt gcccatcttt aaaagaaagt ttatatttat
     5041 tgttacttat ctcagttttg tctatctcga ttgctgtttc taatttttca ttatttttct
     5101 caaattcaac aacagtgtat cctagtgctg gcacatttac atttattttt atgtcaagtt
     5161 cataataagc tggttcatca aaatattcga aacctgtagc aatcatccga taagcatttt
     5221 tccgttctgg aaaataaact tctttttcaa tgatattata gggactacct ttgaagtgta
     5281 ttttctttga atcagctact acatgcacga ttttcgatcc actataattc tcagtcgttg
     5341 tgttgaaaac aaggacttca ttcgatttta gctttaatgc atcagccatc cgttttacaa
     5401 taaggttttc gattccgtca gctatctcat tagcttcttt gattcgatgg aaaatatctt
     5461 ccgcaacatt atctgaaaca cagccaccaa tactatcatg agcctggttt tgcaaaatct
     5521 ttttccataa ccgacgaagt aggcctttac tgatatggat accatttctt tctgcaatca
     5581 gcatgagcgg ctcaactcgt tttagaattt tttgttccaa tttaaaattt tctattttca
     5641 tttttgttct tactgagcca atagaacgat gaacacgaga gtaaaccggc tgacgaagtt
     5701 cacctttata ttcaggtaat tcttttttgt ttctgataac ttctacataa gaaggataat
     5761 ctgaaataat attgtggaat ttactgtatt tattcaactt ctctacgact tgatcaaaat
     5821 ttaaaatcat tgacttttga tctacacccg aaggtactaa aatatctttg ttttttcctt
     5881 gcatatgaag catttcaact gcttcatcca acttttcttt tgcaaattca ggaatatctt
     5941 taattgcgtc taaggtcatc attccagtca tataaccaaa tggaaaattc atggcataaa
     6001 caaatttatc tcccaatcct ttccagataa aataaggaga tggaaccatc gtttcaaagt
     6061 ttaatcctcg ccaacacatg aaattatcga tttctacttg atgcaaaagt gtaggcaaat
     6121 tcgcattgaa cccaaacgtg tcaggcaaat accctaccat cattggatct ccatatttaa
     6181 ggatcgtatc actgattcca atcattaagt ttcttaaaat tgattcagca tccactaata
     6241 aggcatctgt ttgtgcatac cacggtccca cgaatagttg tcctgttttc atcagtcgtt
     6301 ggataacctt aagcttttgc ggattgatct ccaaatactc atcgacgatt gaagtttgtc
     6361 catctaaaca aaagtttact ttagggttcc tttccaattc atctagaact tcagtaaaga
     6421 cctgatcaga caatacgatg gcatcttcat ttgtaaaata ccattctctg tcccagtgcg
     6481 tgtgttgaat tatatgtgca tttaccatat cagattctcc tactttcctt cttctactga
     6541 atttacttgt tgttcttttt tatacttgat ggattttagt gcattgacca ttaacgctgt
     6601 aaccactccc ccaataacaa ttgatactaa atataaaggt atattcgtaa caaaaggaat
     6661 tgccataaag gttgaaagca ttgtttcttg agtaacacca aaaaatccat ttagaccaca
     6721 tgctactgca gtgcccacca cgatgcttgg tattactcgt aaaggatctt tcacggcaaa
     6781 cgggatcgtc ccttcagtaa tccccacaag gcccataatc atagctgtct ttgcattatc
     6841 tcgttccgat tcatcataaa gttttttgtt taaaattgtt gctaaaaaca ttccaaatgg
     6901 cggaatagca attcctaata acatggctgt actaggtcca aagcttccgc ttgaaaaaga
     6961 agcaacccca aaagcatatg cgattttatt gataggtcct cccatatcaa acgccatcat
     7021 tcctcctaaa atagccgaca tcaaagcagc atttgctcct gacatgcctt ctagccaatt
     7081 agtcagtcct gttgtcattg cttgaatagg cgtagctagt acataccata atatgattcc
     7141 aacaactaat gtagtaacaa aaggaatcag cataattgat tttagactaa taacttgcat
     7201 cggtactttt acttttttca atgcattagc taaatagcca caagctaatc cagcaaccat
     7261 tcctccaata aatcccatac caagattttg acacatcatc ccagtgataa aagctggtgc
     7321 tagaccggga cggtcagcaa ttgaatatgc gatataggct gaaatcatcg gtacgataaa
     7381 ctgtaatccc gtctgaccaa tcattcgcag gactccccag aatgtatccg tctggtccca
     7441 aacacctgtg ccaccaaaaa tgactgcaat agaagttagt aaccctgctg ctacgacaag
     7501 cgggatcata taggaaaccc cactttggaa atgatttaga atttctcttc cttgtttctt
     7561 gaattccatt agtaactccc ccacgatata ttttttattt gttaattaat gcaattgctt
     7621 catcaatcgt ctcttcacca tcttttacta cctctgaggt ccctactgtt aaaatcggtt
     7681 taccgtcaaa gcgacctcgt tctgaaacat ttgtatctac tgcaaatatg acaacatctg
     7741 cctcggaaac atccgaaatt gaaagtttgt tttctatccc catagccccc tgtgtttcta
     7801 ctttaatatc taaccctttc ttcttcgcaa acttttctaa attagctttt gccatataag
     7861 tatgggcaat ccctgcgata catgctgtta ctgctactat ttttgtcata tgaaattcct
     7921 ccttttaaaa tgatagagct tttaccaatt cttttttatt tctcgaatct tttattcttt
     7981 taacaaactc gtcatccatc aatcttccag ataattctgc taaaatacgt aaatgattag
     8041 tacctttatc agtgtctttt attgctaaca aaaagacgag atcaactggt tgatcatcta
     8101 atgcgcccca ttcaacagac cgctttaact tggctacagc cactgttgat tcaattactg
     8161 ctaggctttt gccatgagga atggcgatat ttttcccaat tcctgtggtc atatggtttt
     8221 ctctgtcaaa tacgtctttt tgaaattgtt tgtggtcaga cacaaaccca ttagtctcta
     8281 acaatttcga taatttttcg actacacttt ttttatcaga tgcatttaaa tctatcacta
     8341 tattatttat attgattttt tcactgatca tttataagcc ttctttcaat atataaacta
     8401 atttttcaaa cgacgaactc gtttttaaat ttgctaataa ctcatgattc gaagcgattt
     8461 cattgaatgt ttcataaatt ttattcaatt caggttttac tttttcatta agtgcaaaaa
     8521 agaaaacaga atgaacaagt gtcccatccc aatcaattcc ttctggatta acgtagacac
     8581 agattactgg cttttttata aaatctactt cgatatgcgg catagcaaaa tcattcatcg
     8641 ctgttgaaga tagtttttct cttttgattg ctccctctag tatcccttct ttcgcgtaat
     8701 tttcttttac caattttttg gtaattatat ttaaaacgtt cacttgatta tcctttattt
     8761 ggtgggtaaa aaagcaaaat cgtttatcta ataaatttag aaaagcatct tctgtttgtt
     8821 catttaactg tagatactgt tttacttttc tgacatctgc ccctaacatc aaaggactaa
     8881 caacaataac aggaaaattt gtattttcaa tcggaatcgt gcttataaca agttcatcag
     8941 tgacttttct atcttgtagt tcattgatgc ttagtacatc ttctatctca accatatctg
     9001 caaaaccgtt atttattctt tgctctaata attttgatgt tccatatccg ctactacaaa
     9061 ctaatatcac tttcgttttg tcaggtttgt aacgatccaa aagtgattga atatggagtg
     9121 ttacgtaagc gatttcatct tcattaaaat gtatacagaa tctctcttcg atttcctcta
     9181 ataaatctac acttatcata aatgaccttt taaaagaata tttgattttg tccgtataag
     9241 ggttatagat atttactccc aatttgagtc ttttaactgc tgcattgaga tgtaatacta
     9301 gattattgat caattcttca tcaggataaa attcatttaa tttaattctt actaactctt
     9361 gtagctcaac acccatatct aaataggaaa gcttcgtttg atctaagtcg tgattcatag
     9421 catttttttc aatcgaagta atgtgaatgt caacataagc ttgttcatct attggcacaa
     9481 taattgaaaa tgctttttca atcattttga ttaaaataag tgtattcttt gagatttctt
     9541 cttcagaatt attactacgt gataagaaat tgcttgtttc aatcctttct atcgcaatag
     9601 caaggtggat agctaaagtt tgaaattcat aatctgtaaa tttcaattgt gtcttattca
     9661 tgtatcgatt aagcaattcg gctacttttt tcaatatgtc cttatttaag aaattattcg
     9721 cttcaccaat gaaattgata ttcaagtcta agagatctgt tttactgtta gatgctgtaa
     9781 agccacccca ataatagttc acaacttctg acatcaacct tcttttactt ttttcatcag
     9841 ctttgatgcg cacgccattt cgatctgaaa ctaaggacat cccatgcttg tttagtcttt
     9901 ttctaacatc tttcattgtg ctttctaccg tcgacctgct cacgtataat tcgtcagcaa
     9961 gattttgaat ctttagagaa tcattagtat tcaataattt agcaaacaca taagttagac
    10021 ggtcctccgg agagtctgcg atattgattt tatgactgga taataaattt cgaagtgttt
    10081 ctttttttcc aataagcgaa acgccttttt taggttgaac caacatttgt acatctggca
    10141 ttttttggat tatttctttc aaaattaaaa tatctttgct aattgttttc gtagatacat
    10201 ataattgttc tgataattct ttgtaagttg tgtgctcatt ttcaatcaaa aagtatagta
    10261 ttttcatttg ccgttcgcta aattcgatgc ccattatctt cacctcctga atacagctta
    10321 agtgtacaat tattatctct attttcatac agaagaaagt tcttgattaa tgtagaagtt
    10381 tctaatattc ttgattattt caaatcaata aaaaagcctg caatatttat atcacaggct
    10441 tttttctcta ttaaatatat ataagataat acatttttct actagttctc actaatcttg
    10501 tataattaaa ccctccgcaa cttgacggag atggagaaga tgcccccgtc taatctagcg
    10561 gtcatctctc ctcccattcc ttccaccaat ttttttgaca ggtaaagccc gattccgcta
    10621 gattccgtat ttgtgcggct ggtattttcg gtataaaaac gctggaagac tttttcaaca
    10681 gaggtttgcg ttcctggttt gataggattg gccactgtta atcgaatgaa tggatcctct
    10741 tttgaaaatg tcacctttgc ttgtccatct gcataacgca ataaattctg tatgatattt
    10801 tgcagcactc gcagcaacat gagcggatct gctaatacag ttgtatttat cgagtgttca
    10861 gcgaacgcta cctcgatatt tttctcagaa aattgttcat aaaaagagaa aaatacttct
    10921 tccacctctt tagaaaaaga aagcgtatgg agatccattt ctttttgctt cgtctctatt
    10981 cgagcaagat cataaaactg atccgtcata ctgagtaatc ttgcaacaga ttgtttgatg
    11041 atcgttaaat acttctcccg tttctccgta tcctctacct gttccagtaa ttgtacataa
    11101 ccgttaatag cagtcaaagg agttctgaga tcatgagaaa ggccgacgat cgatagtctg
    11161 atgttttctt ccatttcttg aaatgcttgt cctttttgtt cattttcatc caaccatgta
    11221 ttcagtgcat ctactaggtc cattagattc ttttctcgaa attcgactga aagacgacca
    11281 ccatattctt gtcgttcaga aagagtggcg attttcttag ttaatctctt gatttccctt
    11341 tgcaggaaga aaataaaact tcccataaag ataacaacga gaagcaacag ccatttaatc
    11401 aaatctaaac cccactcccc acaccgtatg aatatacaca gcatgtgcct ttgctagctt
    11461 ccctcgaaga ttggaaatat gcatattcac tgttttctca ctatcaaaat atggttcttg
    11521 ccaaactgtt tcataaagat ttgctcgcga aaatactttt ttcggatgtt ctaaaaaaag
    11581 cttcagcaga tcgaattctc ttcctgtcaa atggacagcc acaccattca catttacatc
    11641 cctcgactct ttgtccagta agatttcctt gtaatagatg cgttgcgaca gatcttttac
    11701 ttggacagaa gtctgatgac gaagttggat gccgattcgt aacaaaagtt cttcgatatc
    11761 aaatggtttc gtcacatagt catccgctcc tgctgccaac aaggaaactt tatctctttg
    11821 atcgcttttt gctgaaagaa cgatgatcgg aacattcgat gtctgtcgga tttctgctaa
    11881 caatgcttca cctgaaagcc ctggaagcat gagatctaga aggatcaaat cgacaggata
    11941 tgtgcttaac aataattttc cttccgtacc tgaataagca tcaagaagtt tgtatctctg
    12001 ttcaagaacc tcctccagta aggaatgaac agcttcatcg tcttcgataa tcaaaatagt
    12061 ttccagcagg accacctact taatgtcaga tttttggaaa ataaacattc ctagagctga
    12121 tgataccacg attgttacta gactgaccag aatcattttc ggtgcttgcg aataagttgc
    12181 ttgttgcgca aagtccgtat acatgagcca cgtttccgta aaccactccg ttagaaattc
    12241 caagcctttg atttttgcga aaacaccggg aatgaaggcc gttcccatga tataaagaaa
    12301 agaaagaagc aaactgacac tggtacttct agtgatgaat gtcaaagcca ctggaacaga
    12361 taatgtccct aatgtcagca gaagcaatac aggaaatact tttagaacat aatttccata
    12421 gtttttgatt ccctcaactt caccaaatcc gaaagcaagt gtgtaagcaa gcatggctag
    12481 tcccgtgatc aaaaccacgc caagtgttgt gatcaaaagc gtaacgagaa gtttcgacac
    12541 ataataagtc attcgattcc gtcctaaaga taacgtattg cgaatcgtac cgttttgaaa
    12601 ttcttcccca atataaaagc acgcaaatgc tgcgaaagcc agaagaatcc atgcagccat
    12661 tttttctacc attaaaatcc ctgttgcacc gttgatgact gtatccgttg cttggtttaa
    12721 ttcattgccg tattttgtat gcatatacca ttcgttccaa gaataaaaaa gcggaagtac
    12781 gaccatcagt cctagtgaga cccacatttt acggctcttc catactttta ttttttcagc
    12841 agttaataga ttaaacatgt ggattgcctc cagttaattg taagaaataa tcttccagtt
    12901 tttgcccaac taggtgaata ccatccacag gaacttttgc ttctaccaat gcttgattga
    12961 tggctgctac ttggtcaatt ccttcaaaaa gctggataac acggctagat tgtacaaaat
    13021 aatccctata accaagagag tcgagaaccg ttactgcttg ttccgtcgcc gaagtgtcaa
    13081 tttttataaa ttgacgagat tcttgtgcca attcttcttt cgataattct ttgatcaagt
    13141 gtccttcatg caaaatacca tagtgagtgg cgatctgtga caattcatct agcaagtggc
    13201 tagaaagcaa gacggtgatg ccccgttctt ttaccaaacg ttggatgatt tctcgcattt
    13261 ctgccatccc tgctggatcc atcccattcg tcggttcatc caaaatcaaa aattctggat
    13321 tcgtgatcag tgcatttgca atagctaaac gctggcgcat acctaaagag aaattctttg
    13381 ctttcttttt ccctgtattt tccaatcgca tcattttcag caggtcttcg atttctctat
    13441 ccgacacacc gccgttcgct gcttggatac gtaggttatc tcttgcagtt aattctggat
    13501 aaagagcagg ggtttcaatc gattgaccca tacgtcgtct tgctgcctgc aaatccgtcg
    13561 ttccaaataa ttcgatcgaa ccttcatcaa tcgagatcaa acccataata gttctcataa
    13621 aggtagactt acccgctcca ttttccccaa tcaatccata gatcattcca cgtttgattt
    13681 cgattgatac tttatctaat gctttggcag caccgtattt tttggttata ccagttgctt
    13741 tcaagaccgt ttcatttcca aaatccatac ttatccttct ttcttattga tgagtgaagt
    13801 atagttgacg ggtataaaga aagcagccag aaatgtataa agaaagtgta aattttccaa
    13861 ttgaatgtca aaaaaaacaa tcggacaata cgtgtctttc ctattatcag gcagtttgtt
    13921 atacgcttgt atcagtttcc tgattctttc ttttcgacca aagtcgtttc tctgtattcc
    13981 tcttcactga aaatttttgc ttctttcaga tcaagcttga gtaattttac cagaccggtt
    14041 ataaggataa ctatagcaag actccctccc agaaaaatta gactgtctcg cagacgtggt
    14101 tgcgttgtct tccaatcaaa gagcgcacca aacagtgcta atccaagtgg actggccacc
    14161 atggtaatcg ttgttatcgt tgacataagt cgtccttgta tatgcgttgg aacttctcgc
    14221 tgaagccaga ccatataaaa agcaaaagaa aacattaatc cttaaaaacg gacttatgaa
    14281 aaataataca tgccgtttgg gatttacaat tagctaccaa ataagaggtt atgacaaaag
    14341 acttttcaca atcccttctt ccgaataaac ggtgttctcc tgccaactcc tcggtcacaa
    14401 gtcacaatat ttatcaaaca tgagaagcaa tttttgtaaa ttccttctta tttcttgaag
    14461 ctgaccactt ctgtcacaac ctcagaagcg gataaataat attgtttgat gatactttat
    14521 ctttttcccg tttgtttata gatcgaatga tattatagac caagaatcct acctattaac
    14581 atagctaaaa acacaaaaaa ggcactttcc aaattgtgga aagtgccaga aaagttagga
    14641 ttaaggtaac tgcgctcagc attgcttcgc ccagtacgat tctcgtaact cgtgaactcg
    14701 aacgataatc gcaattaacg ttttgagaac tgtgaagctt tacgggcttt cttaagacct
    14761 ggttttttct tcgtatcatt gaataagttt gaaaaacctt gatttataaa ggttttcccg
    14821 taaatatatt ttattatatt tgattaaatt tgaaatgttt gtgttctaaa atgtgttcta
    14881 atttaaaggc agtatatttc gtattttaga ttaaattttt aggctcattc attctcaaat
    14941 ccattctatt cagtcaataa aaattttaaa aagactttat aaacaaagat gttcaagcga
    15001 gcatcttatt tttttgtgct taatttgaaa ggagaacatt tcatgtataa aaaacaaaaa
    15061 aagaaattat gtgtattaac tattttgagt ttggctgttc aagcaattgt tggagcaatt
    15121 gctcctacga ttacttttgc tgatgaaatt acccatccac aaacggtaac cgtagaacta
    15181 gatttagcac atcaatatgc tgttgaagga acttttagtg atggtcgtcc aatgtcagaa
    15241 gtcactgttc cgcattatgc agtgtacaac ggctttaaac aagatatttt ctgtattgaa
    15301 ccaggagtcc cgatttacaa tgagttcact cctggctatg agaaaaaccc gttaccagat
    15361 atgtccgata aagccaaatt agtttccgtt ttatggaaaa aagtaggtac agatgcagat
    15421 actcatattg tggcacagaa gatgatctgg caagaagtta atgggtatac ccttcactca
    15481 ataaaacgat cagatggtag tgccgtaaat atcgcagcca ttgaggcaaa aataaatcaa
    15541 gccattgacg attatcagaa gaaaccaacc ttccataata gtacggctaa aacagtatta
    15601 gggcaatcga ctactgtgac ggatacgaat aatctgaatt tgtcagagtt cgatgaggtt
    15661 gtggaaaatg ccgcaaacat tgattatcgt gtgaatggta atcagttgat aatcacccca
    15721 aatgccaatt caaaagaaag tggtgtgctg acgcttaaga aatctgctgg taccggaaca
    15781 cctgtcgcct ataaaaaggc tggccaacag actttaatgg ccggggcgat tgataaacct
    15841 aatacctaca caatcaaaat tgatgtggaa acggagggtt ctttaaagat caaaaaagtc
    15901 gataaagaat cgggtgatgt tgtaccagga acggttttcc atttagactt tggaaaaact
    15961 ttacctgcaa aagacgtgac tactgacaaa gaaggcattg ctacattgga tgagattcct
    16021 catggtacaa aagtaaccat taccgaaaaa tcagtaccgg cgccttatac gattgatacc
    16081 acccccatga ctaccaccat taaagcaggc gaaaccattt acgtgacttc aaaaaacgca
    16141 cgggaaaaag ggcaaatcat tctggataaa agcggagttg aaacagggag tgatctttgg
    16201 aatgacaatt attccttagc tggtaatacg tttgccattc gcaaagacag tccaaccggt
    16261 gaaatcgttc aagaaatgac cacggatgaa aacggtcatg cggaaacacc aaaagagatt
    16321 gctaatgcgt tggaattggg aacctattat gtgaccgaaa ctaaagctag tcatgggttc
    16381 gtgaatacgt tcaaacctgt aaaagtcgaa ttgaagtatg ccaatcaaac cgtagcccct
    16441 gttaccagta acgtaaaagg gcaaaatcaa gaagtcactg gcgaaaccac tttgacgaaa
    16501 gaagacaaag atactgggga taaagcacaa ggtaaggcag tctttgaggg tgctgaatac
    16561 accttgttta ctgctaagga tggaaaagct gttaaatgga gtgaggcttt taaaccagag
    16621 ttagcgaagg gaacgaaagg ttctgatgaa acggtcactt tgactttaga cgaaaagaac
    16681 caagttgccg ttaaacattt agctatcaac gagtattatt ggcaagaaac aaaagcgccc
    16741 gaaggatata gccttgatga aacaaagtat cctgtatcca tcaaaaaggt ggatgatagc
    16801 gaaaaaaatg ccgtgattac ccgaaatgtt acggcaaaag aacaagtgat tcgctttggc
    16861 tttgatttct ttaaatttgc tggatcagct gcgggcacta ccgaaactgg cttcaatgat
    16921 ctgaccttta aagtgtcgcc attggaaggg accaatgaaa tcacaggtgc cgaagatgaa
    16981 gcgaccacag cttataacga gcaattaggg tttgatggtt atggcaagtt tgaaaatctt
    17041 ccttatgggg attatttact tgaagaagta gaagccccag aaggttttca aaagattacg
    17101 ccattagaaa tccgttctac ttttaaggaa aacaaagaag actttgtgaa gagtgagtat
    17161 gtctttacca ttaccgaaca agaccaaaaa caaccaatta aaacggtgac cgttccttac
    17221 gagaagctga cgaacaaagc attttctgtt agtttgaacc gtttaatgct ctatgatttg
    17281 cctgaggaag aagatagttt aacttctctt gcgacttgga aagacggaaa caaagaatta
    17341 acttctctgg attctaccga gctagttgat aaattgagtt acaatctaca tgaaatcaaa
    17401 gaagactggt atgtggtagc gcaagccatt gatgtggatg cgacaaaagc tgcccaagaa
    17461 aaagatgaaa aagccaaacc agtggtgatt gcagaaacaa gcgcaacgtt ggcgaacaaa
    17521 gagaaaacag gaacttggaa aattcagcat aaattaaccg ccgaacaagt tttaaataaa
    17581 actattgtct tattcaacta tgtgtatgaa aataaggaag cttttgaagc aggcgataag
    17641 ccagtagcga aggatgttag cttgaataac caagcccaaa ctgtcagttg tacagtggaa
    17701 caccatgttt ctatccaaac aaaggcccac ctagaaaacg gttctcagac ctttactcat
    17761 ggtgatgtgg tggatatgtt tgatgatgtg tcgattactc atgatgtact ggatggctca
    17821 aaagaagctt tcgaaacaat tctgtatgct ttattaccgg atggtacaaa caaagaaatt
    17881 tggaaatctg gcaagattga ttatgaagta aatgataaag aattcaccaa aactgtgctt
    17941 gcgaaaaaag tagataccgg aaaatatcca gaaggaacca agtttacttt tgcggaaatc
    18001 aattacgata aagatgggaa tatcaatgga aaacacaatg aagatttgaa agaaaaatct
    18061 caaaccttaa caccaaaaga agttccaacc atactaagta cgccgaaaca accggaaaca
    18121 ccaactgttc caagtggctc tcaagaatct agtcccacag tgaagacctt ccctcaaacc
    18181 ggggagaaaa attccaatgt tttactgttc attggtttta ctttgatttt tgcgacggcg
    18241 ggctattatt tctggaatcg ccggaactaa ggtgatgaga tgatacaagt aaatcctaag
    18301 aaccaacgtg cgcgaccgcc tcctaaaaaa gtagtcgcgc tatttttgta tccaaaacta
    18361 acaatgaaaa gaggaaaaga agatggaatt gaaatttgtc gtgccagata tggctgaaac
    18421 ctttggaaag ttaagttatg ccggtgaagg agaaattata acagaaggat atggacggaa
    18481 tactacggtg attggtcgca gttatcattt gtattccagt aagcaacggg ccgatgatat
    18541 tgaggtggta gtagctgcgg aagccggtga aaaggatttt gatcaagacc aaccgctaaa
    18601 agccgtgaat ccccatttgg ttgccaaagg ctatgagatt gagaatcgtg ggtttactga
    18661 ttacgtgttg tatgttgatg atttagtgaa agcataggag gaaaagaaga tgagattaac
    18721 agaaggtatt gtcgtagatt caggtttaac atttgggaag ttgcgttttt ccgcattacg
    18781 ccgagaagta cggaagcaaa atgaggatgg aacaattggt aatgaggtaa aagaacgaac
    18841 ctataattta aaatcctctg cacaagggcg aatgattcaa gttagtatcc cagctaatgt
    18901 acccttacgc gagtttgctt atgatgcaga ggtagagttg gtcaatccaa tcgtggatac
    18961 cgttgccaat tatgtttttc gagaaggaac taccgttaat tggttcatta aggcggatga
    19021 tttagtcttg aagcggcagc cgaatcaagg aaatcctacg aaccaaaatg aaagaaagaa
    19081 ataggtgaag attatggaaa ttaatgttga acatatttta gattgtctcg atcaatatgg
    19141 aaaaggcgag ctgactgagg aacaactcgt taaagcatta acctctgatg aaaaaatgtt
    19201 tctgattata catcaaggtt tacttggaga aaatagcgac agtgaagaag actctgatgt
    19261 actagactgg ttaggagaag aagagtcttt ctttatagtt gtcgaagtaa atgaaaactt
    19321 atgtcggcag gcagaatcca ttttggaaga aattggtgtg gagatgccgg atgctattga
    19381 aggcttttta aatcaattgg ttgaaacgaa acaactcccc tcctcgtagc agtaaatgac
    19441 tagcataact ttttgaaatt gcctaatgaa atcctattgg gctttttcga ttggaggtgg
    19501 aaaccatcaa aatgtacaaa ggacatcgca tacgcgcagg agatcagcac ttggtttatc
    19561 actttgttct tggctggcta ctcgctctgt tcatcggttg gatgagcgtc ttttattttc
    19621 aagagtttag acaatttgat atttctaagc tatcactttc tactattgaa atcgtttggt
    19681 ctataaaaga tttagtatgt ctgcttggaa gtctcgcttt ttcaggagct atgatattgc
    19741 tttatattca cttttttctt gatcattggc gaagcttgtg gcatcgacaa aagctagctc
    19801 ggatgattct agagaaccat tggtatgaag tgaaacaaac tcaaagtgaa ggctttttca
    19861 aagatttgaa tagtagccga accagagaga ctatcagtta cttccccaaa atctattatc
    19921 gaatgaaaga tggattactg tctattcgcg ttcaaatttc acttgggaaa taccaagatc
    19981 agcttttaaa attggaaaag aagttagaaa gcggactgta ttgtgagtta gtagaaaagg
    20041 aactcaaaga ctcctatgtg gagtatactt tactgtatga tatgatcgcc aatcgaattg
    20101 ggatagacga agtagtggca gaaaatggga ctctgcgatt gatgaaaaat caagtatggg
    20161 cttatgattc tttaccccat atgttaatcg ctggtggcac gggtggaggg aagacctatt
    20221 tcctcctcac cattatcgaa gcactattga agtcagatgc agaattgttt atcctcgatc
    20281 ccaaaaatgc agatttagct gatttaggta cggtgatgcc tcatgtttat tcacaaaagg
    20341 aagaaatttc tgcttgtgtg gaagatttct atgaacgtat gatggctcgt agcaaggcaa
    20401 tgaaagaaat gtctaactac aaaacaggag agaattatgc ctatcttgga cttccaccca
    20461 actttttaat ctttgatgaa tacgtggctt atatggagat gttaacgacg aaagaaagtg
    20521 cggtgatttt gaataagctc aaacaaatcg tgatgttggg tcgtcaatct ggtttctttc
    20581 tgattttggc ttgccaaagg ccggatgcga aatatttagg agacggaatc cgcgatcaat
    20641 tcaactttcg cgtggctttg ggtcgtatga gtgaactcgg atattccatg atgtttgggg
    20701 aggtcgaaaa aaacttcttt atgaagcaca tcaaaggtcg aggttatgtg gatacaggtg
    20761 gcagtgtgat tagtgagttt tatacgccac ttgtaccaaa agggtatgat ttcttagatt
    20821 caattagtaa attagaaaag atgaatgatt gagtgatctt caatactgac tttatacttc
    20881 aagtaacgta taatactttt atatagtagg aagaaagggg ataattaata tggaaagtaa
    20941 tgattcttat ttagagtatg caaaagcaaa taaggataag tttatttctt atattatcaa
    21001 agggaaatcc cccgaagttg gacgagacgc agtttttatg gcagggagtc ctggggctgg
    21061 aaaatcagag gttgccattg gattagctga gaattatgat aaccatgtga ttattgatgc
    21121 ggattacttt cgcacccagt ttcctgatta taatggaaaa aactctagtc tctttcaaaa
    21181 agcatcttct tggttggtag agcaggcatt tagatatgtc ttagatcatg gctattcttt
    21241 tattctggat gcaacttttg caatattaag tgctgaaaaa aatatctcgc gggcgttgaa
    21301 aaaacatttt cgagtgacga ttttctatgt ttatcaagac cctaaaatcg cttggaaatt
    21361 tacacaagaa cgtgaaattg cagagggacg tcatgtgccg aaaaaggcat ttattaacgc
    21421 ttttttcaaa tcaagagaga acattgcaaa agtaaagagt aaacatccag atgtattgct
    21481 tcatatcata ataaaagatt atacgaataa tatttccgaa gttcattatg ctacggataa
    21541 tctagaatta tttttaccac tagggtatag taggaaagat ttggaggttc aattacatga
    21601 ctgagaaaga acaagtaacg aaaattgtaa agaaatacaa taagagcatt gcggatttat
    21661 cggaaaatgc tactgcaaaa gagttcaaaa cggtgattaa atatgtagcc gatcaggcca
    21721 atgaaaaaca acggaaatta gttggattaa ataaaaaata gtaggacaaa ttaataatgt
    21781 atgagccaag agcaaaatgt tcttggcttt tttctttgca atgaatgagg taatgtatga
    21841 gatttttaga tttattcgcc ggtattggtg gctttcgttt aggtatggaa caggcgagcc
    21901 atcattgtat cggtttttgt gaaattgata aatttgctag acgtagttat aaagcaattc
    21961 atgatacaag taaggaggtg gaaatgcatg acatcacaag tgtatcagat gagtttattc
    22021 aatccctcgg accagtggac atcctttgcg gcggatttcc gtgccaagct ttttcaattg
    22081 cgggaaagcg gcaaagattt tccgatactc gaggtacttt attctttgaa atcgctcggt
    22141 tcaccactct tctccgacct aagcttttat tccttgagaa cgtccgagga ttgctcaatc
    22201 acgaaggagg ggctacgttc gagacgatcc tccgaacgct ggatggattg gggtatgacg
    22261 tggagtggta ggtgcttaac tcaaaggcct acattcccca aagccgggag cggatattcc
    22321 ttatcggaca ttctcgagac gcatgtaccg aacaagtatt tcctatcatt ggaccgtctt
    22381 caacatctga tcaaaacatc agaaatttgg taaatatcaa tccttccaat cgaggaatgg
    22441 gtggacaagt ctatggttca gatggcgtag cgccaacctt aacaggtgat gaaggaatca
    22501 aaatcgcact gccagcaagg gatggaatct ctgtagcagg actattaccg ggaaagtttg
    22561 aacaaggtaa tcgagtttat gaagttactg gaaccgcccc caccttgtca acgaaacaag
    22621 gtggtacaaa aatcatgatt cgaaaaaatg aaacagaata tcaagaggtg aagccagaag
    22681 acagtgtgaa tctggctttt cctaattcca caagtcgccg aggtcgtctg ggaaaacaga
    22741 gtgttcatac cttactaaca ggagaccaac aagcgatcgt cacggatcag tatcaaattc
    22801 ggaagctaac acctagagag tgttggcgac ttcagggctt tcccgattgg gcttttgatc
    22861 gagattctca agtcaattca gatagtcagt tgtataagca agccggaaac tctgtaacag
    22921 ttccggtcat ttttgatatt gccagaagat taaaagagtt ggatgtacat ggtttatgat
    22981 gagttgattg gagaaattta ttgggtgatt ggaaagattc agtcagaccc agaattagaa
    23041 gaacaattgc gacggttaaa atttgatatt cgcaagaatg gtgtcaaagt cgctggcgat
    23101 ccatatttaa tgaatgagga gacggatgct cgaattgaaa taaatcaagt aattgcagaa
    23161 tttgagcgga ttgcggacca agcaaaagaa ccagatattc gtcaatactt gttagaaatg
    23221 aaagcagagt tggaagtaaa tggtattaca gatgagtaac ctaaaccaac acaactgact
    23281 gttaggcgct gaccgcggag gattcgcgaa gcagaaatcc tccgtggtgc tgtgcctgtt
    23341 catggacggc gttagccgac catgatgtct cacccccgta tctaacaggg gggtacaaaa
    23401 acacaaaaaa taagtaagca accattaaga atgcttgttg tatcaaagat agtaagtaat
    23461 ttgtataggt gttaactcat ttgaaaaagt taacaccttg aatttggagg tgagaacttg
    23521 gcacaacgga atttagatta tcggctatta aaagaccgac gcaacgaata cggcgtttca
    23581 caaaataaat tggctacggc ttgtggactt agtcggccgt acttaaatca gatagaaaat
    23641 ggtggcgtaa ccgcatctat aaaaaccatg aggaaaattt ttgaccaact ggaaagcttt
    23701 aatcccgatt tgcctttgac gttactgttt gactatgtaa ggattcgctt tcccacaacg
    23761 gatgcacgaa aaattattca agaaattctc catttgaaat ttgagtatat gctccatgaa
    23821 gattatgcct tttactccta tcaagaacag tatgtcatgg gtgatattgt ggtgatgttg
    23881 tctcatgaag aagataaagg tgtgctttta gaattaaagg ggcgaggttg ccgacaattt
    23941 gaaacttttt tactcgctca aaagcgtagc tggtacgact ttttcgagga ttgtctaaag
    24001 gccggtggcg tgatgaaacg cttggacttg gcaatcaatg atcgagtggg gctattggat
    24061 atacctgagc taacgaaaaa atgtcagaag gaagaatgta tctccttgtt tcgtaccttt
    24121 aaaagttatc gttccgggga acttttaaag gctgatgaaa aggacggcat gggcaatacc
    24181 ttatatatcg gcagtctcaa gagtgaggtt tatttctgtt tgtatgaaaa agactatgaa
    24241 caatatatca agttagggat tccattagac caaactgaga cgaaaaatcg ttttgagatt
    24301 cgactgaaaa atgaccgtgc ttttcatgcg attcaagatt tgttaaaagg tcgtagcatt
    24361 gagagcacca cgttttccat tatcaatcgt tacttgcgat ttgcggataa agtagaaggt
    24421 aaaaggcgaa ccaactggcc cttgaatgaa caatgggggc gttttattgg acggaatcgg
    24481 aaagagattc aattaacttc cgagccaaaa ccgtatacca ttgaacgaac cttgaattgg
    24541 ctaggccgac aagtcgcccc tacgtggaaa atggctaagg agttggatcg attgaatcgg
    24601 acaacctata tccaggatat ggtacagaat gcacgcttat cagaccgaca taaaaagatt
    24661 ttggagcaac aaagtatggc aattgaaaat ttgattatat gaaaagagga atcgtcatgg
    24721 gaattgtaaa agatattttt ttggtatttg gtggcagtat gcttggtgtt actgtgatgt
    24781 gcttgatgaa tgcaagtgca gcagcggatc gagcaatgga aaagaaagga aagtgacaag
    24841 atgaatttcg gacaaaattt
//
LOCUS       DACDYI010000048        24322 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.048, whole genome shotgun sequence.
ACCESSION   DACDYI010000048 DACDYI010000000
VERSION     DACDYI010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.048"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            6..980
                     /locus_tag="IWM52_001799"
     CDS             6..980
                     /locus_tag="IWM52_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP9974047.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
     gene            1710..2027
                     /locus_tag="IWM52_001800"
     CDS             1710..2027
                     /locus_tag="IWM52_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974048.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNASIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(2231..2596)
                     /locus_tag="IWM52_001801"
     CDS             complement(2231..2596)
                     /locus_tag="IWM52_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974049.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            2804..3286
                     /locus_tag="IWM52_001802"
     CDS             2804..3286
                     /locus_tag="IWM52_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974050.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            3483..4373
                     /gene="pstS"
                     /locus_tag="IWM52_001803"
     CDS             3483..4373
                     /gene="pstS"
                     /locus_tag="IWM52_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAP9974051.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            4698..7169
                     /locus_tag="IWM52_001804"
     CDS             4698..7169
                     /locus_tag="IWM52_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9974052.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(7272..9008)
                     /locus_tag="IWM52_001805"
     CDS             complement(7272..9008)
                     /locus_tag="IWM52_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAP9974053.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(9005..9709)
                     /locus_tag="IWM52_001806"
     CDS             complement(9005..9709)
                     /locus_tag="IWM52_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9974054.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            9840..10343
                     /locus_tag="IWM52_001807"
     CDS             9840..10343
                     /locus_tag="IWM52_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAP9974055.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            10303..10641
                     /locus_tag="IWM52_001808"
     CDS             10303..10641
                     /locus_tag="IWM52_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAP9974056.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            10662..12080
                     /gene="ffh"
                     /locus_tag="IWM52_001809"
     CDS             10662..12080
                     /gene="ffh"
                     /locus_tag="IWM52_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAP9974057.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            12192..12770
                     /locus_tag="IWM52_001810"
     CDS             12192..12770
                     /locus_tag="IWM52_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAP9974058.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            12774..13295
                     /locus_tag="IWM52_001811"
     CDS             12774..13295
                     /locus_tag="IWM52_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9974059.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(13374..13928)
                     /locus_tag="IWM52_001812"
     CDS             complement(13374..13928)
                     /locus_tag="IWM52_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP9974060.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            14181..14456
                     /gene="rpsP"
                     /locus_tag="IWM52_001813"
     CDS             14181..14456
                     /gene="rpsP"
                     /locus_tag="IWM52_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAP9974061.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            14468..14719
                     /locus_tag="IWM52_001814"
     CDS             14468..14719
                     /locus_tag="IWM52_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP9974062.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            14844..15635
                     /locus_tag="IWM52_001815"
     CDS             14844..15635
                     /locus_tag="IWM52_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974063.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            15805..16326
                     /gene="rimM"
                     /locus_tag="IWM52_001816"
     CDS             15805..16326
                     /gene="rimM"
                     /locus_tag="IWM52_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAP9974064.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            16316..17077
                     /gene="trmD"
                     /locus_tag="IWM52_001817"
     CDS             16316..17077
                     /gene="trmD"
                     /locus_tag="IWM52_001817"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAP9974065.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            17213..17560
                     /gene="rplS"
                     /locus_tag="IWM52_001818"
     CDS             17213..17560
                     /gene="rplS"
                     /locus_tag="IWM52_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAP9974066.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            17818..18111
                     /locus_tag="IWM52_001819"
     CDS             17818..18111
                     /locus_tag="IWM52_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAP9974067.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            18954..21281
                     /locus_tag="IWM52_001820"
     CDS             18954..21281
                     /locus_tag="IWM52_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9974068.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            21407..21736
                     /locus_tag="IWM52_001821"
     CDS             21407..21736
                     /locus_tag="IWM52_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP9974069.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     AEIKTSD"
     gene            21776..22780
                     /locus_tag="IWM52_001822"
     CDS             21776..22780
                     /locus_tag="IWM52_001822"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP9974070.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKTKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            22777..24206
                     /locus_tag="IWM52_001823"
                     /pseudo
     CDS             22777..24206
                     /locus_tag="IWM52_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
BASE COUNT         8334 a         4274 c         4812 g         6902 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtca ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgatga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt ttcttgatgt gatatccttg tcttttttgt
     1081 aatgttttta acttattgct tttgcaaaat ttatttacct gttttctgga tcaacgatag
     1141 tgtatattat gctatccttg tttggatttt aggacgttta aagaggcact tttttacata
     1201 acttaatcca tagacctttt aagcatgctg tgagataata ataaagaaga gtttaaagcg
     1261 caccccaaac cacttcccca taagtgtatt acgctttaaa ctcttttata tttgaattca
     1321 ctaagaagca taccatattt ttgaaaaaaa gtgagaaaaa aggcttataa ttaggattta
     1381 gagtaattaa ttagatgttt attttatagg actgatatta tagattacaa atagtatcgt
     1441 atttcgcaat cttaaaatta tcttcttact atgaataaga taataaaatt tgtatttgtc
     1501 ctttttaggt aacttatttt aatctatata cctttctgga tcaacgaaag tatactttat
     1561 atagtcataa cgccgatggt aactatgcac tgtaacgata atgctgctaa aatatctttt
     1621 tacgaaaaac atcacaagga aaaacttaac tatggtatta tctaaacata attttttcgg
     1681 tatttaatat ttaattagga gggaatattt tgctttttta tagtaatttt atcttaatag
     1741 tagcgatatt attacttctg aatatatgga tttttgatag gagtagaaat gcaagtatag
     1801 gttttagaac taaacgaagt ctgtcttcta aaaaaaattg ggtatactca caaactattt
     1861 tttatggagg aattgtttta attagtttac tttcttcaac actttattct ttgaatatta
     1921 tagatgtttc cacctcaaat tcaatatcta ttattggaat cattattgct gcaattatta
     1981 cacaactgtt tttagtgttt ggagagaaga aaagaagtaa aaaatagaaa aactagtgat
     2041 agttttggtg atagtgatat taaaaatcag aaaagataga aaaagcagga gatagataaa
     2101 atgctgatat ttcagcattt tttaagagta taaaagagaa aaaaagtaat aaatgtatac
     2161 atgatataag aaaaacatat atgtataaaa agaagaaagt agcttctctt tgtacatata
     2221 tggtttgttt ttatggatgc cgagcttttt tataagataa ggctaccgcc cataacgacc
     2281 caaggatttg tggaactaag taaatacctg caagaagcaa gatcagctgg gtcggtttta
     2341 aaggatgata gaagtaacgc cattcagtaa aattggcgca aaataaatag gaagtgacag
     2401 tagatataat attggcaatg aataaagata taatacgcct gttcctttga aaataatatc
     2461 cagcaaaaag agataaaaat atcaatgtga tgaacccgct tacggaatga taacgaaaat
     2521 caagcagtaa ggcgaaatgc acataaggaa caaaagataa aagcaacgga aggtttttcc
     2581 agcaagtttt tatcatcttt catcaccacc taacaagtgt tattattttg ttagttttat
     2641 tgtaaattga atcctgcaat aatcaattac tttttcatta tttctagatt aaatgaataa
     2701 actctcatgc cattatctct aaaaaaaaag tttttccccc taattttttt agatagaaaa
     2761 ttgtatagta tgatgagaga aaaagtagtt tgggggaacg ttaatgagaa gaaaagcagt
     2821 aatcgatcgg ctttatttat ttttttggtt ttttcctatc gttttgctac cttttacatg
     2881 ggtaaatgct gttgtacaag gtgcggagat attaggcctg atccaaggaa gcggatttgt
     2941 ggtttctagg aattttccag ctttgactgt tttctattta gtgataagtg taggtctctt
     3001 attaccaaaa tctctgatca atcactacaa ttggcagctg ctttactttt cttgcttatt
     3061 agtatttaca tctatgtttt ctttgctgcc tcgcttgatt atcggcccta atggtttgag
     3121 cgagagtgga ttgaaggatt cagcttattc ctccatgtat ttcttgacga tcaaaccatg
     3181 gttctattta gtaattgcct catttattct tgcaacattt ttttacttct ggacagaaat
     3241 caggataaaa agtgaacgat ctgtatctat aaaaaataac tattgatggt ggatcatcag
     3301 taaaaaagca tatgagctgc gtaaaatgca tgtttctcat atgctttttt tattattatg
     3361 taaagaatgc gaaaaacgtt tacatgattt ttacataact taactatatt tttacagcaa
     3421 ccatacgagg gatttacagt cgactgataa actttagttg taaataagaa aggagcaata
     3481 aaatgaaaaa agtattgttt actctaggta tgatgggaat tcttttagcc ggatgcggct
     3541 caggatcagg aaatacagat ggctctgcca cacaaaataa ttcaagtgac tcaaatgaac
     3601 aagtaaaaat cgttgcggta ggttcgacag cattacagcc gttagtcgat gcagcacaag
     3661 aaagctttgt acaagaaaat ccaaattatc aaatctctgt acaaggagga ggaagcggaa
     3721 ctggtttgag ccaagtcgaa gctggagcag ttactatcgg aaactctgat gtatttgcag
     3781 aagaaagaga cggagttgat gcttcaaaat tagtggatca taaagtagca gtggtcggga
     3841 tggcgccaat cgtcaataaa gatacagatg tcaaagatat caccaaacag gaattgattg
     3901 atatctttac aggaaaaatc actaactgga aggaagttgg cgggaaagat caaaaaatca
     3961 acgtcgtgaa ccgtgcaaac ggcagcggta cccgcgcgac attcgaaaaa tggggactgg
     4021 atggtgctac ccctgtgcag tcccaagaac aagattcatc aggaacagtt cggcagctgg
     4081 taagtcaaac accaggagca atcagctatt tagctttctc atatctcgat gattccacac
     4141 aagcactaag tatcgacggt gtagaaccaa aagaagagaa tgtagcagat aacagctggg
     4201 gaatctggtc atatgaacat atgtacacga acggaaaacc ttcccctgaa gtccaaaaat
     4261 tcttagacta catgatgaca gaagaaatcc aagaaggacc tgtcaaagaa ttgggctatt
     4321 taccaatcac aatgatggaa gtagaacgtg atcacgaagg aaatatcaaa taactagcag
     4381 ataaacatgc cacggtagtc gttactatta aggaaaaatc aactatgttt cccacatata
     4441 catttttcct ttaattgtga aaaatagttc ctcatatttt gccgtttttt gagctaatac
     4501 cgagggtcag ctgattaaac accgtttatt cgaaaaaaag agctacgaca tagaactgtc
     4561 gtagctcttt ttttgctata tgacaggaat gaaaggtgtt tgcaagctta atgtgaggaa
     4621 cactttccat atctgttcta tactggatag gagaagtttg gaaatatcca gaaaacgaaa
     4681 tcaatcgagg tagccacatg aagaataaat ggataaatcg aagtaaaaaa aagaaagtaa
     4741 cacaaagatc atcagataaa gtcatccgtc tttttccatc gtataaagaa ggattaacgg
     4801 cagatcaagt caaggaacga atagaaaaag gtgcagcaaa taattcagtc gatcccacat
     4861 tcaaaacaaa tcagcaaatc gtattggaaa acatatttac gtatttcaat ttgatctttc
     4921 ttatcttagc cattttgctt tgtttagtag aatcctataa gaacttgact tttttaccag
     4981 ttatcatagc caatacagga atcgccattt ttcaggaaat tcgatccaaa aagatcttgg
     5041 acgaactaaa tgttttaaat acagcaaaag tcaatgtcat acgggacggg atagaacaaa
     5101 caatagatat agaagacctt gtaatagatg acattgttat tttagagact ggacatcaaa
     5161 ttccagcaga tgcagaggtc gtggaaggaa aactgcaagt taatgaggct ttattgacag
     5221 gcgaagcaga tgaaataacc aaagaaatag gcgatccatt aatgtccggc agtttcattg
     5281 tttccggaaa agcttacgcc cgattagaca aagttggagc agattcgtat atttcccagc
     5341 tgacagtcaa agccaagaca atgggagaag gagaacagtc ggagatgatt gcttctctta
     5401 atcaattgat caaatggatt gggatcatca ttattcctat cggtatcctg cttttttctc
     5461 aaagttactt ttttaatgga aacacgatca aagaaagtgt cgttgcaatg gaagcagcat
     5521 tgatcggtat gatacctgaa ggactgtact tgctgacgac gattgcatta gcattaagtg
     5581 cgactaaact agcaaagcaa agagttcttt tacacaatat gaaaagcatt gagacattgg
     5641 caagggtaaa tgtcctttgt gtagataaaa caggaacgat cacagaaaat aaaatgtctg
     5701 ttcaaaaagt gatcgtctct aaaaaacaag aaataatcag tcagtcagat caattggaag
     5761 catgcattgc tgactatgca aaagcgatgg caggagacaa tgcaacaatg gaagctgtta
     5821 aagcatattt tacaaaaacc actgatcaat cctatcacca tgttattcct ttttcttctg
     5881 tccaaaaatt cagcagtgta gcgttggcag acaaggtgta tgttttagga gcgcccgaaa
     5941 tggttttgcg cgatcaattg ccggaatatg cggaagagtt tgtttctttc gctgaaaaag
     6001 gctatcgcgt gttggtcttt ggtgtgtacg atgggattct tgaagaacca gtattgacag
     6061 aagcagtcac accattaggc tatattctga ttgctaatcc tatcagaaaa gaagcaagag
     6121 ccacatttga ttacttcaaa aaacagcaag tagccatcaa agtgatttct ggagataatc
     6181 cgctcaccgt atccaatgta gctgtccaag cagggatttc acatgctgat cagtacgttg
     6241 atgcatctaa attatcagaa gaagaatatg aagcagctgt cgaaaaatat acagtttttg
     6301 gacgcgtcaa gccagaacaa aagaaaatct tcgttcaatt gataaagcgt aacaatacag
     6361 tagctatgac aggtgatggc gtgaatgata ttctagccat gaaagaagca gactgcagta
     6421 tagcgatggc ttccggaaat gaagccgcca tgcaggcctc acaagtggtc ttacttgatt
     6481 ctgacttttc acgcatgcca gaagttgttg cagaaggacg gcgcgttgtg aacaatattg
     6541 agcgatcagc cagcttgttt ttagtgaaga atattttctc ctttttgctc tcactgtttt
     6601 cagtgatctt tgctctgacc tatcctttag aaccatcaca aatcacattg attagtttgt
     6661 ttactatcgg acttccatct ttcttgcttg ctctagaaga aaacaaaaag cgaatcagag
     6721 ggaaattcat catgaatgtg atggaaaaag ctgtgccagg tggactaaca gatatgatcg
     6781 tagtcggtgc gttggtgatc tgcggggtga ctttgaatct aaataaaaca gatgtttcta
     6841 cagcatctac catgcttttg attgcagtag ggttcctcgt tctctataaa atctgttcac
     6901 cgctgaataa attcagaagt cagatcattc ttttctgtgc tagcggtaac tttttttcgg
     6961 tcatcttttt gcataagctg ttttcaatca ccggtatctc agctgtatca cttttgctgt
     7021 tgagcattct atttttctcg gcagattcga tttttaggca tttgaccaca ttggtagaat
     7081 atttgtttga acgtaaaaat gaaaaagagc aaaagccgtt tagtcgcgta gctaaaaaat
     7141 ttctatcgat atttaaacga gaaaattgac gaaaagtatc tcaaaaaaga gtaaaaaaga
     7201 acaaccactc gtgtatcttt cgaagcagga tgagtcatcc aactttaaaa cgcacgagca
     7261 gttgctcttt tttacatttt cggaatggta acggtgaaag tcgaacctgt tccaagatga
     7321 ctgtctacag taatcgtacc attaaggagt tgcacgtagt cttttacaat cgctaaccct
     7381 aatccggtcc cgcctgaatg tcggcttcgc gccttgtcta cgcggtaaaa acgctcaaag
     7441 atccgttcct gatcctcaat gtcgatacct ataccaaagt cttggacaga aaacgataga
     7501 tcatctgttg cctgatagcg gataataatc tcgctgtcgc tttttgaata ttggacagca
     7561 ttttcaatca gatttttgat aattggataa aggatatctg ttttagtagt aaatggttcg
     7621 ttctttccaa tcaatcgaat cgtcaaccgt ttcttttcga tgattggctg ataattttga
     7681 acgatttgct ggaaataagg ttctgtataa attgtctgga tctcgtaaga attctcgcca
     7741 tcttttgaca gctggatgat ttcccgaatc agtttatcta accggatcgc atctttttgc
     7801 atgatctcaa gaaaagaagt cagagtttgc gggtcatctt ttgcaccatc cagtaaagtc
     7861 tcggtaaaac caatcagtga agtcacagga gtttttaatt cgtgagagac gttgccaacg
     7921 aaatcctttt gtagcttttc taaacgtcgc actttcgtta agtcatacgc cacccctaga
     7981 atctgtccgg taccttctga attagtaaag tagcgcaaac tcaagtctaa tgtctgccga
     8041 ccttctgtaa gggtgatctc ctgatggatc agcggatgct caggagtcac ttgatgaatc
     8101 agctgaatga gttttggctc ttgaatgact tctgtgtacc gctgttcagg ttgatagctt
     8161 accacgttta aatgtagctg catctttgga ttaagcagta gtaaacgaga atcagcatca
     8221 tcaatcagga atacgccaat catcagttcg ttcagtaacg tatataattg ctcttctgtg
     8281 gtagtacacg cccgatacat cttactcatt tgttcgctca acgcattaat cgtctgatac
     8341 aattcttccc attgatcggg tgtttgcatg attacttccg cctgattagg attttgcacc
     8401 atttttttta gtacgggcag cacggttttc aatggctggt ttttttgata gatcagataa
     8461 tagatcattc ctgcaagaac aaggaagaaa gacagaaaga agaaaaagac ccaacggcga
     8521 aaactctcag ttctcggtaa aaaaccgctt gtcggttctg cgatacgtaa aatggcttct
     8581 aactggccac tttttttgac tggtaatgcg acatataaaa gatcctcatt caaagtagta
     8641 cttttgcgca aagcgctacc taggttccca ccattcaata cggctttgat ttcgggacgg
     8701 gagtttcgct gttcattcaa tgtttcattg tttgtgtcaa ataagatctt tccagtagca
     8761 tccattaaag taatccgttc gttcgactga tggacgaatt cttccaaagc agttttgttt
     8821 tgcgctgctt ggaggttttc gacatctaat tgccggatca gcagttgtgc tttttttgtc
     8881 aggtagcttt cttgctgttc cacgatctgc tgttgaaaat aatgggaaat caattgccag
     8941 ccaccaaaaa acaaactaac taaaagaata agaaagaaga gacgttcata aatttttttc
     9001 catttcatcg tttgggttcc tgaaacttat agccaaatcc tctaatagtc accaagtact
     9061 ttgggtgttt tggatctcgc tcgatttttt ctcgcaaatg actaacgtgt acatctacga
     9121 ttcggctttg tcctgcgaag tcaaaattcc aaatgcggtc tagcaatgtg tctcggtcga
     9181 tcacacggtc ttttcgtttc atgaaataaa caagtaattc aaattctttc ggtgttagtt
     9241 caatcagatg gtcgtctacg gtcacttgat aattggtcaa atcagcagtg atttgtccaa
     9301 tcgatagata ttcaggctca gcttcatctt ttgattgctt acgaggttca attcgtctga
     9361 atattgcttt catacgggct aatacttcgc gaggactaaa tggtttggtc aaatagtcgt
     9421 cagcacctat ttctaggcca atgatccgat ccacttggtc atctttagcc gtcagcatca
     9481 aaatcggtgt ttcatttttt tcttgacgca gccgctgagt gatctccatg ccatccatcg
     9541 ttggaagcat cacatctaaa atgatgaaat caaatgattg ttctaatgct aattctaatc
     9601 cttcgcctcc atctgtggca ctggttacct tgtagccttc tttttccaaa ttgaacgtca
     9661 agagcgttac aatagaaggt tcatcatcaa caactagtat ttttttcatt caaatctcct
     9721 ttaaataggt ttcattctgt ttatatttta gcatagaatc agtacaaagg gctattttac
     9781 aaaagaaata aatggtataa taggaaggca ctttgctgaa tagaaaggaa gagcagtcaa
     9841 tgattggaat cagtgcatgt ttaggcggtc tggcctgtcg ctatgatgga cagaaaaaag
     9901 aagtaccaga attgatggaa ctagttgaaa ctggagaagc tgtcatggtc tgtccagaag
     9961 tgatcggcgg attaccaatt cctagagatc ccgctgaaat cattggtgga aatggattcg
    10021 atgtgtggaa agatcaagca aaagtatgga cgacaagcgg cgaagatgta acggaagcgt
    10081 acaaaaatgg cgcgatcaaa gcctatcaaa aattgcagga aaaacacatt gatctagtca
    10141 tcatgaagga aaaaagccct tcatgcggaa cccattcgat ttatgacgga agtttttccg
    10201 gaataaaaaa aaatggtgcc ggagtggcta ctgcttattt tattcagcaa aaaatgactg
    10261 tgctctctga tgaagaatgg ctgaaaacta gaggtgagca gaatggaaat cgaaaagacc
    10321 aatcgaatga acgcgctctt tgaattttat tcgaccttat tgacagaaaa acaaatgaac
    10381 tacatggaat tatattatgc agatgatttt tcattaggtg agatcgcgga agaatacgaa
    10441 gtaagccgac aagcagtata tgacaatatc aagcggacaa gcaagatctt ggaggattat
    10501 gaaaaaaaac tccatctttt ttctaattat attgttcgag aacaggtatt ggaaaatatg
    10561 actgcatata ttacagataa gtacccggaa gataaaaaac tacttgaata tgtacagcaa
    10621 attcaagcaa tcgaagaata aataaaagga atgaagaaaa tatggcattt gaaagcttaa
    10681 ctgaacgtct ccagcaggcg atgggaaaaa taagaaaaaa aggaaaagta tcagaagctg
    10741 acgtaaaaga aatgatgcgg gaaatccgcc ttgccttact agaagccgat gttaacttgc
    10801 aagtagtcaa agattttact aagcgcgtac gcgaacgtgc ggtaggggca gaagtgctgg
    10861 aaagtttatc acctgcacaa caaatcgtaa aaatcgttga tgaagaattg acgatcacac
    10921 ttggttctga aacagcagag ttaaataaat caccaaaaat accaactgtt atcatgatgg
    10981 ctggtcttca aggggcaggt aaaacgacct ttactggtaa attagccaat tatttaaaga
    11041 aaaatgaaaa tgctcgtccg ctcctaatcg caggggacgt atatcgacca gctgcgatcg
    11101 accaattgaa agtattaggt caacagctag acgttcctgt atttgatatg ggaactgaag
    11161 tcagcccggt agagatcgtc cgtcaaggga tggagttagc aaaagaaaag aaaaacgact
    11221 atgtattgat cgatacggca ggacgtctgc atatcgatga aacattgatg gatgagttga
    11281 aacaaatcaa agaattgacg caaccaaatg agattttgct tgttgtcgat gccatgactg
    11341 gtcaagatgc cgtcaacgta gctgatagct tcaaccagca attaggtatt acaggggtcg
    11401 tcatcactaa acttgatggg gatactcgag gcggggcagc actatcgatc cgctcagtga
    11461 caggcgcacc aatcaaattc atcggttctg gtgaaaaatt gactgactta gaagtattcc
    11521 acccagatcg tatggctagc cgtatccttg gtatggggga tatgctgaca ttgattgaaa
    11581 aagcgcagca agactacgac gagaaaaaag cggaagaact tgcacagaaa atgcgggaaa
    11641 attcattcga tttcaatgac tttattgaac agcttgatca agtgatgggt atgggcccac
    11701 tggaagatct aatcaaaatg atcccaggaa tgaatcaagt ccctggaatc gaaaacgtca
    11761 aggtagatcc aaaagatgtt gaacgcaaaa aagcgatggt ttattctatg acgccagcag
    11821 aaagagcaaa tcctgatctg ttgaacccaa gccgtcgtcg ccgaattgct gccggttctg
    11881 gaaacagtgt tgttgaagtg aatcgaatga tcaaacaatt caaggaatca agaaaaatga
    11941 tgcagcaaat gtccaaaggg gatatgaaca tcccaggtat ggaccaaatg tttggttcag
    12001 gcgtcaaagg aaaactaggc aaaatggcga tgaatcgaat gatcaagaaa aataagaaga
    12061 aaaagaaaaa acggaaataa gatgaaaaaa acagcttcgg tacagactga aaatgtctac
    12121 tgccggagct tttctatcag caatttttat tctttactaa tacaattact ctaaacgaag
    12181 aggtggcaca gatgagatgt ccgtggagtg aacaaacaga aagtatgcgg caatatcatg
    12241 atactgtctg gggtgttcct gaatatgatg accaaaagct gttccgaaaa ttgatgcttg
    12301 atattaatca agccggattg agctggcaga cgattttaaa taaaagtgaa gcattcgatg
    12361 aagcatatga tcgattcgat atcaaaaaag tcgcaaatta tggagaaaca aaaatcagtg
    12421 aactaatgga aaacaaagga attattcgta accgaagaaa aatcgaagca gccgtccaaa
    12481 acgccaaagc agtcttgaaa attcaagaag aaatcggcag tttttcagag tatctttggt
    12541 cgttttctaa tggaaaaatc gttgattctt cttatcattc acaagaagaa gtcccaacaa
    12601 caagtcctct ttcagatgaa ttagcaaaag atctaaagaa aagaggattt aaatttatcg
    12661 gaagcacaac aatctatgct tttttagaag ctgtcgggat catcaatgat caccttgaca
    12721 gttgtttccg caaagaagag gtcgtacgta tcggcagaaa gggcgaataa agcatgcgaa
    12781 aaatcatatt ttatggtgct ataagtttag atggctattt ggcaacgaaa acggatgatc
    12841 ttcaatggct ttttgacact ccaacaggta agcaaactac ttatgaagct ttttatgaaa
    12901 caatcgatac gaccatcatg ggcagaaaaa cttatcagga agcaaaaaaa tatttggaga
    12961 ccgaagcaat ttatccagag aaaaagaatt tcgttttttc cacccacgca caattacaat
    13021 tgccagatgc tgaggtcgta aacgaagatc ctgttgcttt tctaaaaaat ctacaaatga
    13081 cagaaggaaa agatatctgg gttgtgggtg gcggtcgatt gatcaaaccg ctattggaac
    13141 atcaaatgat tgatgaatgg tatatccaga ttacccctgt attattaggg gacgggattc
    13201 ctttattcca aaaaggaaca tatgaagcgc gttttgaaat gctagacacg acacgattcg
    13261 gtgaatttat cgaattgcat tatgtacgaa agtagaagta aataaaataa aaaaggcaac
    13321 tatatgcaga aatgtcctgt acatagttgc cttttttatt accgttatgc tttttactct
    13381 gttaatttgt tagaaaaatc tagatacgca tctaaaatcg tttggaagaa acttaatgtt
    13441 ccttcaacga tattaccatt ttcatcaatt agattcgcaa cgtttccgat atatgcttcc
    13501 ggttgctgca aggttggcac attcaaaaag acaagtgatt ggcgtagatg gtggtttgcg
    13561 ccaaagccgc cgatcccacc tggtgatgca gtaacgacta aacctggttt atgatcccaa
    13621 acactgtgtc cataaggtct tgagcctaca tctaaagcat ttttcaatac tgctggcaca
    13681 gaacggttgt attcaggagt aacgaaaacg acaccgtcta gtcccctcac ttcctcacga
    13741 aaacgagtcc attcagcagg agcctgttct ggagtttcga gatcctcatt gtaaaatggc
    13801 agatcatcga tttttacaaa tacactttcg tacccttcag gaagcaattt cgcaaaagct
    13861 tctgctactt ttttgttgta agaatctttt cttaaactac caataaaaaa tccaattttt
    13921 tttgtcatac gatttttcct ccttcaaact atctataata atcttacaaa agataagtaa
    13981 ctggagcaaa taaaaacact tactgacaaa agctacatag aagatagatt acttattttt
    14041 aacaaaaaag aagtctaaaa tcttctattt tctaaaaaaa atcgaattta atcaagctgt
    14101 aaagaaaaaa agctttacag ggtaaaaaaa aactgttaca ctacatcttg tgaataaaac
    14161 aatggaggtg gaaataaata atggcagtta aaatccgttt aaaacgtatg ggttctaaaa
    14221 agagtccgtt ttaccgtatc gtcgtagctg attcacgttc tcctcgtgat ggacgtttca
    14281 tcgaaactgt aggtacttac aatcctttga aagaccctgc agaagtagtt ttaaaagaag
    14341 acttagttct tgactggtta tcaaaaggtg cacaaccttc tgacactgta cgtaacatcc
    14401 tttcaaaaga aggcgttatg caaaaacacc acgaagctaa attctcaaag aaataaggtg
    14461 accggatatg aaagatttaa gcgacttagt cttaacaatc gttcgtccgc tagtcactta
    14521 tcctgatcaa gtcaagttag acgtcgtaga gtcgaaagac ttttacgagt acaatctaac
    14581 agtggctcct gaagatattg gccgtatcat tggaaaacaa gggcgtgttg ctaaagcaat
    14641 ccgcacaatc gtttacggtg ttcggatcaa tgcaccgaaa aaagtgcgtt tgaatatcat
    14701 cgatgataag gaaaagtaaa taaacgtgct gtactcgaat tttcgagtgc agcttttttg
    14761 ttttttttat taattgaaga aatgattatt tggcaaaaca agcaagaaag cgtatcctta
    14821 taggaggagg gagtgaacag ccaatgagaa agaaaatgtt atttattcca tttgctctat
    14881 tgttaagcgc ttgtagttcg gttactcaag aatcagacaa tacacatcta tcaagcagta
    14941 tatctgaaac tgttaccagt ccagaaaaaa gtagtaccaa aacggcttcc accactgaac
    15001 aaacaatggc tacctcaaat aatgagaaaa agactgcttt agaccagttg aaagaacaac
    15061 agccaaatgt tcccatgcct ctagatgtac cagtctcttc aggctatctc aatatcgcag
    15121 cgacacatac aaagcaaggt tattctattt tatattacag aacagatcgt cccctcgggt
    15181 tgaatgctga cgaattgaat caagaaacgc ctattgcaac ctatctatat caatatgggt
    15241 ttgcttctag tcaagaaaca atccaagttt tgcagccatt tgagatcgac acaaatggtc
    15301 agcaagtgga cttaggttct cgaatcacag ggtatcagca aggtgcagca ggttcaagtt
    15361 ttcttgaatg gcaagaagga aactggcgta ttcgaatccg tgggaataat atagaaggcc
    15421 aagatccctt actattagct aaagaaatcg tagcttattt ggaagaaaac agtctaccag
    15481 cacctgaaca atttggtaaa attactgttg atatgggtga tacgacgaat cgagccgtag
    15541 aagtaagctg gcaagaaccg aaaaatgcct atacgatcac tcatcaagat cctatgtctg
    15601 cgttgaagat ggcagtttct atgaagaggt tgtgaaatca gcgcgctgat ctgagcccat
    15661 catttaactc caagtcacag gaagtaatca aaaatagcac gccgcatttt tgcataattt
    15721 tggcttaata ccttaaaaga aaactagaat tgcctcttca cacatgctaa aataaaacag
    15781 acaaaaaaga aatggggata taagttgact gaatacttga atgtaggaaa aatcgtaaac
    15841 acacaaggta tcaaaggcga agtccgtgtt atctcaacaa cggatttccc agaagaacgt
    15901 tacaaaaaag gaacggtgct aacactattc caagacggaa aagcacctgt ggaattaaca
    15961 gtcaaaagtc atcgcaaaca taaaaatttt gacttattga gttttgaagg acatccttct
    16021 atcaatgatg tggaaaaata tcgggatggc atattaaaag tgtcaaaaga caactctgta
    16081 gatctagctg aaaatgaatt ctattaccat gagatcatcg gtctgcatgt attagaagat
    16141 ggaactgagt taggtaaagt aaaagaaatc ttgtcaccag gtgccaatga tgtttgggtc
    16201 gttcaacgtc cgaaaaaagc tgatatattg cttccatata tcgcttctgt cgtcaaagaa
    16261 gtcgatttag aagctggagt ggtccgtgta gaaattccag aaggactgat tgacgatgaa
    16321 gattgatgta ttgactttat ttccaagaat gtttgaaggt cctatggggg aatccatcat
    16381 cggtaaggct gtagataaag gattattaga tatcaatatc tctaacttcc gcgattattc
    16441 agataacaaa catcagacag tagatgatta tccatacggc ggtggagcag gtatgctgct
    16501 caaagtccag cccatctatg ataacatcca agccattgaa aaagctgcac cggatgtcaa
    16561 aaaaagagta attcttcttg acccagctgg taaacgattc gatcagaaaa tggcagaaga
    16621 attttcacaa gaagaacact tagtattcat ttgtggtcat tatgagggat atgatgaacg
    16681 tatccgcaca ttagtgacag atgaagtttc cttaggagat tatgtcctta ctgggggaga
    16741 attaggtgcg atggtcatga tcgatgcaac agttcggctt ttgccagatg tgttagggaa
    16801 tcagacttca gctcaaacgg attcttattc aaccggttta ttggaacatc cccaatatac
    16861 tcgcccggca gaatataaag ggatgaaagt accagaagta ttgacaaatg gaaatcataa
    16921 attgattgaa gaatggcaat taaaagaatc actaagaagg acctatcaac gtcggcctga
    16981 tcttttggaa acacttgaat ggacacctca aatgacgaaa tttttagaag aaatcaagaa
    17041 agaagaacaa gaaaaaacag cagatgaagc caagtgactt tttctaaaat cttctttaca
    17101 gtaggttgat aatatgttag tattttatgg tgagtgcaaa agcacttaac tattacgata
    17161 ttccgctgtg agaaaagctc ataagaatat ttggaataag gagaagaaaa ccatgaatcc
    17221 attaatcgaa gaattaacaa aagaacaatt acgttctgac atcccagcgt tccgccctgg
    17281 tgacactgtg cgtgttcacg caaaagttgt tgaaggtact cgtgaacgta tccagttatt
    17341 tgaaggtgtt gttatcaaac gccgcggtgc tggaatcagc gaaacttata cagtacgtaa
    17401 agtttctaac ggagtgggcg ttgaacgtac attcccattg catacaccac gtgttgcaaa
    17461 aatcgaagta gttcgctacg gtaaagtacg tcgtgcaaaa ttgtactacc tacgtgcatt
    17521 acacggaaaa gctgctcgta tcaaagaaat ccgtcgttaa tcattgttat cagactgaga
    17581 aagaaccctt ggtattcaag ggttcttttt tcttagcatg taatttgaac accctgatcc
    17641 agttttttct ttaattattg gtaggggaga ataatggatt agaataaatc tacctataag
    17701 tagtagtaaa aaaaagagca ctatgataat atctttctta agagtaaccg ctttaataaa
    17761 aaatccagaa tattctaaca taaatggagt attcttagaa tcaaaaggag aaagaagatg
    17821 gtttatactt atacggagca agaaaagatg aaagttttag acacattcat gaatgatgga
    17881 aaagtaaaac aagtaccaag ttcaacaaaa aagaaataca tcctattgaa agaagtcttg
    17941 aaacgctttg accatggagt gacttataca gaaacagagg ttaattcgat actgctgaac
    18001 gtattttcta gtggcgatta tgtcgaacaa agacgttatc taatcacatt tggctttttc
    18061 aaaagaagtt cagacggtcg ggcatatcag atgatgggaa ttgaaaatta gaagttttaa
    18121 tttatactca ttgaaaaaag ttcaagtatg cgatattggt tctacaccgc atacttgaac
    18181 tttttttgtc atctgtatct ataatacaaa gccaagaacg ttattcaaaa tatggatagc
    18241 tatgataaaa gctaatgagc gattcgcagt taaataagca atcgtcagta aaatcgttcc
    18301 aggagcgtac atgaaaaaat caattggatg cgttacatga ataaagataa ttctctttta
    18361 tttgtacgaa taaataccaa aacgtacctc ctaaaattag attttggaca gatgcccttt
    18421 aaaaagataa caagatttcg ttcttgaaac aatatatatg cttttattcc aatgaggttg
    18481 tgaaaacagt attttgtcct gagcgttaga aaattgaaaa tggggaatag caactcgtac
    18541 tttaccattt tttaagtgaa tacaagatca gctaattgaa caccgtttat tcgtgatgag
    18601 ggacacaaca ctgcagtcga cacatttctc ttttatgttc aaagataaca aatatttaaa
    18661 acaaagaaaa atagtagata atcccgtcct aactctttta ttcttccctc atagcttcca
    18721 aatttaagca atacttcaat taatcaagtt ttatcttttt ttttcaataa tcgtaaaaaa
    18781 agagtatttt ttatttaaaa ataaaatctt tgtattaaag gacaagacta aaataacctt
    18841 agaataaaaa gagaaaaaaa gccccaaact ttctatacat caataaaata ataaaaatta
    18901 aatttagaat aacgtgtaaa ggattatttg atgatttttt attgagagga gggatgaaaa
    18961 tgattaagaa aactcaaaat aaaatccaac gtatatgtca aaaacatttt gtttctagac
    19021 tcaaagctgt atctaaaaga aaatacttac atcccattat gatttcgatc atcttgattt
    19081 ttactttctt agggagctta tcaagtattt atgtaaacgt ttatacagaa acgtcaaatt
    19141 ttgctaacca aacgacagag gagacaaata gtccacccta ttctgaaata cagtccatag
    19201 aaagcaccaa tcagtctagc aacaacgaga caacctcaac cacatcctcg actcaactga
    19261 ttgatccgac agaaaaagaa atcacagcag caaaagcagc aggggagaaa gcttattcag
    19321 aaacaggtag aagccagaca tttagtactg ttgcggccac tgcgaatttc aacggacata
    19381 ccataccaga tggatataca gtgatatatg ccaaagatgg agtcattgtt gcggttaacg
    19441 gatcgatgag tgcggataaa actgcgttac aaggcgtaac caaagcaatc gcttttattg
    19501 ataaccgtcc agatatccat aatctgttta ttggctccac agtaaatggt actactatca
    19561 cattcccaaa aagtatctat atgggggtgg ctaccgctgg aaatacgatc gattcagtta
    19621 cacccaatgc ggataaaagt tattctttta caccctcaac atggggaggg cttcctgccg
    19681 gaggaggtat gagtactttt tcaatcggta tacagttagc aaatgatatt ccattcacgg
    19741 ggtactcgat caaaaatggg cagatcgttg atggtaaggg aaatgtttca aattttccaa
    19801 cctatcaagc agcaacggtt caggtcaatg cgaatcggac aggaactttt tcttatacag
    19861 tcaatggtgg agcagcaaaa atcggaacgt acgggactac attatctgga atccatgtgg
    19921 gtgacaaagt ggttatcact ccaacagcag taacgggcta ttcttttgca caaacaaacg
    19981 gttctttcac agccctagaa agtaacgtcg atacggtgac ctatacaggc aaacaaacac
    20041 caatcactat taagtatcaa gactattttg gacagatgat tgctccttct aaaataatga
    20101 atcttactta cggaagtgct gcacaagacc tgacgactaa tacgcctact atcaacggat
    20161 atacctttac agctgtttca gcgacagaaa caaagaacca atcagcaatg agtgttttag
    20221 ctagtttaga tacaaatgga aatgtaatcg taaaggacgc aaatggaaaa cagatttctg
    20281 aagtcatcct atattataag acaaatgcaa cagtatcaat caatgcaaat ggcagtaagt
    20341 actatgatgg tctttctgtt gtaccgatcg ttaaatatgc ctttaatgat aaaacagaat
    20401 caacatccct tttaaacaat gatctcagta ttgatcaaat cacatggaat ccaactgatt
    20461 tcaaagcgat gaatgaaaag ggagttgaag taactgcgcc tactgaaatt ggaacctata
    20521 cgtcttggca attgacaaaa agcggactag ctaaactagc tgccagaaca aactatttat
    20581 ttactatcgt gcagacatct gatgtctata cgattaagca aatcagcggt gcggtagagc
    20641 tcagtggcag taagacatat gatggaaaag caggtgtccc gtcaatccat gtcaagctag
    20701 cagagggtgt cactagttcg cttactccag tacaagtggc acttagcagt atggattaca
    20761 cagcagatgc acaatcagca aaaaatatgg ttaatgctgg cagctataca attaaactaa
    20821 cgaatagcgg aatagacaaa gtcaaagcag caaatcccaa gttgtcattt actgatatag
    20881 ccaacacatc aggaacttat accatctata aagctaacgc agtgattact gtcgatgacg
    20941 ctgcctttaa ttatgatgcc caaagtcact cgatttcggt tggaaatctc cacgttaccg
    21001 gtgttgtctt aggcgaatca ttggattata ccctaacagt taacagccgg acagatattg
    21061 gatcacaaat agtaacgatt agtttaggcg aaggaagtgt aaacaataat tatacgatta
    21121 tcgcaacaga taccgctaag ttgacgatca atccgactct cttgcttcct tcgacaggtg
    21181 ggataggtct actgcccttt gtctttttag gactgatctt tattttttct ggcttctttt
    21241 attttatcca tcgtaagaaa aaagcaggtg aacagcgatg aaaaaaacaa aaaatgtacg
    21301 tgtggatata tttgctgttc tcttttttat cctactggtc ttttttatag gttctccgac
    21361 tgtgtcagct agagaatcac ctattacatt tactttgcat aatcgaatgt tcgaaagcga
    21421 taagatgcca agtatcagtc aaaacgacgg aataaatatc ccggataact ttcaaggagt
    21481 aaaagggttg aacggcgtta ctttcgctat atatgatgtc tcggatgaat tttacaagct
    21541 gcggtcagaa ggaagtagtg tggaagacgc acaaagaaaa ctagcacaaa aaagtgatag
    21601 tgaaaaaata ctggcagaaa atgtgacaaa aacagtagac gaggaagagg gcattgcttc
    21661 ttttagtgca tcggacaaag atgagcaagg tcgtgacgct gtttaccgat ttgcggaaat
    21721 aaagacttca gattaagtaa aagaaaaatc agcaccattt gtcgttgtgc ttcctgtgac
    21781 agattcatct aatcataaat tgacgaccat ccacttgtat ccaaagagcg aacaaaaaat
    21841 acctgctgca ctcacattaa caaaaacagt agagaataag cagacagatt ttgcagatgg
    21901 ggataaagtt ccttatctca tcaccgtaac aattcctgag aacatcaacg agataggaac
    21961 ctatacgatc aaagatacag ctgatcctca gttatggcta gaaccagaaa cgatcgattt
    22021 tttgattggt ggagacaaga ttcatacatt tcacacacaa aaaacaaaac atggttttgt
    22081 cttatcagat tctggaaaag atcttagttc gtttgctggg aaaaaattaa cgatccgtta
    22141 ccaaatgaca ttgaaagaaa acagtgaaaa aacgacattt gataacgatg tacgtttgac
    22201 gactgacaat caaacagtag aaacacatct tgccatccaa acaggaggca aacaatttgt
    22261 caaagtggat cggcaaacga aacaaaaact agcagatgct caatttctag tcaaaaaaaa
    22321 tgaccaatac ttagcgcaag aaaacgggat aaatcattgg gtagccaaag atgattcaag
    22381 agcaacgatt tttacttcca gtaaagatgg aacattttct gtccatggat tgtcttttgg
    22441 aagttacgaa ctagtggaga ttaaaccgcc taaaggatat atattgagtc aatcaaccat
    22501 tctctttgaa gttgaaaaag gcagttatga tccgcttcat cctataccat tggaaatcat
    22561 caacgaacca aaagcaaatc cgacgaataa tggaaaaacg ccagttcaga tgcagacaaa
    22621 tggtccatca aatgaaaaaa atggaggaag ctttccaaaa acaagcgaag agatgcttgg
    22681 aggtttctca attttagggt tgttgctagt cctttcaaca gggacagctt ggttttataa
    22741 aaaacaacaa aaaggaaaca ataggaggga aataaaatga aaaacatcaa aacaaaaata
    22801 tggacgtttt taggaactgc tatcatgctg cttcctttcg ttctcggcct aggaactgca
    22861 gaggtttcta ggccgtatca cctacaccag aaaatgttac ggttaatttg cataaattga
    22921 aattcacttc agcaccagaa aatcaaataa acaacggaac ggaattgaca tttccgaata
    22981 gcgaaccgtt gaacggcgtt gaattcaacg tatatgacat cactgctacg tactatccaa
    23041 gtaaagacac agcagttcca gcagacgcta cacccttcgc aagtgtaacg acttctggtg
    23101 aaggattagc taacctgact cttccaggta aatctgatgg gaaagatgct gtatacgtct
    23161 ttgtagaaac accaaagccc ggtgtcgaaa cgtcgccaaa tatcgtgtta agcttaccct
    23221 tttataaccc gaattcatca gaccctgaca aacctttaga cacgattcac ctatatgcta
    23281 aaaatgtcat caaagaaagc aatatcaaaa tccagaaagc tgggaacttt tcaggggtag
    23341 ataagttagc aggggcgcaa ttccagatca aaaattcaga gaatcaattc gtgaccggtt
    23401 ttgatgaaaa cactggtgtc gctctctatg atcctgctga gtcagcgttg acattcacaa
    23461 ctgatactga aggatccatc agtgtcaatg gattaataga cggcaattat actttagttg
    23521 aaacaaaagc accagatggt tatcaattaa ccaatccaca aaaagaaact acttttatag
    23581 taaaaaacgg aaaattgcaa caaacaaccg gtatcatcca tggcgcagat gaagccaatc
    23641 ttgggaatcc aaacggtgta gtcaatcggg aaaatacaac tacggataac acgattactg
    23701 tagaaaatgt ccaaaactac ggtgatttcc acttcatcaa acaagatgtg aacacgcaag
    23761 aaaaattagc cgatgcagaa ttttctattc taagagcaaa agatccaact gttaaattaa
    23821 tgaaaaaaat agcaaatctt gcctcaggag aatacccata tgcttggtca gatgaagtca
    23881 caggttctga ctgggaagaa gtgaaattga tttctgattc agaagggaaa ttttcaatcg
    23941 aagggctgaa acaaggggca tactgccttc aagaaacaaa agcaccagaa ggctacgcat
    24001 tacctaagaa tgaagctgcg tacactgaat ttacagtaga tgatgatcca acaacttctg
    24061 atgaaacaac gatcgataac caacctaaag gaatcttgcc ttcaactggt ggtatgggga
    24121 tcatcgtatt catcttggta ggtactgctt tagttggagg agcagtgatc tacttcaaaa
    24181 aacgccatca agaaacagaa gcttaaacta ttggaaacaa aaatcatacg ataaataaac
    24241 gatagtgatt caaatgggga gaaaggttgt gacaagtggt cggtctcttg gtattcgctc
    24301 aacaaacggc aaaataggag ga
//
LOCUS       DACDYI010000049        23867 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.049, whole genome shotgun sequence.
ACCESSION   DACDYI010000049 DACDYI010000000
VERSION     DACDYI010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.049"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            163..1026
                     /locus_tag="IWM52_001824"
     CDS             163..1026
                     /locus_tag="IWM52_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9974071.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1155..2777)
                     /locus_tag="IWM52_001825"
     CDS             complement(1155..2777)
                     /locus_tag="IWM52_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9974072.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3026..3547
                     /locus_tag="IWM52_001826"
     CDS             3026..3547
                     /locus_tag="IWM52_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974073.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3733..5463)
                     /gene="spxB"
                     /locus_tag="IWM52_001827"
     CDS             complement(3733..5463)
                     /gene="spxB"
                     /locus_tag="IWM52_001827"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAP9974074.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5628..6446
                     /locus_tag="IWM52_001828"
     CDS             5628..6446
                     /locus_tag="IWM52_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAP9974075.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6549..6746)
                     /locus_tag="IWM52_001829"
     CDS             complement(6549..6746)
                     /locus_tag="IWM52_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974076.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6915..7493
                     /locus_tag="IWM52_001830"
     CDS             6915..7493
                     /locus_tag="IWM52_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP9974077.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(8538..9041)
                     /locus_tag="IWM52_001831"
     CDS             complement(8538..9041)
                     /locus_tag="IWM52_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP9974078.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9400..10425
                     /locus_tag="IWM52_001832"
     CDS             9400..10425
                     /locus_tag="IWM52_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9974079.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            10748..11626
                     /locus_tag="IWM52_001833"
     CDS             10748..11626
                     /locus_tag="IWM52_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974080.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12102..12218
                     /locus_tag="IWM52_001834"
     CDS             12102..12218
                     /locus_tag="IWM52_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP9974081.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13146..13484
                     /locus_tag="IWM52_001835"
     CDS             13146..13484
                     /locus_tag="IWM52_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974082.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13578..13808
                     /locus_tag="IWM52_001836"
     CDS             13578..13808
                     /locus_tag="IWM52_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974083.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14208..14579
                     /locus_tag="IWM52_001837"
     CDS             14208..14579
                     /locus_tag="IWM52_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP9974084.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(14853..15545)
                     /locus_tag="IWM52_001838"
     CDS             complement(14853..15545)
                     /locus_tag="IWM52_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9974085.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15548..16333)
                     /locus_tag="IWM52_001839"
     CDS             complement(15548..16333)
                     /locus_tag="IWM52_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9974086.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16570..17970
                     /gene="lacG"
                     /locus_tag="IWM52_001840"
     CDS             16570..17970
                     /gene="lacG"
                     /locus_tag="IWM52_001840"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP9974087.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            17995..18312
                     /locus_tag="IWM52_001841"
     CDS             17995..18312
                     /locus_tag="IWM52_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9974088.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18325..19998
                     /locus_tag="IWM52_001842"
     CDS             18325..19998
                     /locus_tag="IWM52_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9974089.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            20012..21643
                     /locus_tag="IWM52_001843"
     CDS             20012..21643
                     /locus_tag="IWM52_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAP9974090.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(22226..23428)
                     /locus_tag="IWM52_001844"
     CDS             complement(22226..23428)
                     /locus_tag="IWM52_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9974091.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(23431..23625)
                     /locus_tag="IWM52_001845"
     CDS             complement(23431..23625)
                     /locus_tag="IWM52_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP9974092.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
BASE COUNT         7367 a         4042 c         4769 g         7689 t
ORIGIN      
        1 ttttcgtaaa ttccttctta tttcttgaag ctgaccactt ctgtcacaac ctcttatttt
       61 gattagaact tgcgaaaata aaccggttaa agtgtgtcta gcgcaagaga tttgctataa
      121 tagacgataa gggtaaaatg taacgagaag gggttgaaaa atgtgtatca aattgtaaca
      181 gattcttgtt gtgatctgcc ataccatgta ttagcaaaag aaaaagttga ttttttatca
      241 atgaatctcc aattgaatga tcaagagtta gtagatgacc tcggaaagac attcaattat
      301 gaccgctaca tgaatcagct gaaaaatggt gcgatgccta cgacatcaca aatcaatgtt
      361 ggccgatatt tagaattttt ccgttcttat gcggaaaaac agattcctgt tttatatgtg
      421 gctttttcca gtggattaag cggctcttac agtagtgcgc ttcaagcggt tgaattgctg
      481 aaggaagaat atgaagaccc tgagatctat gtttttgata caaaagctgc aagtattggg
      541 gaagggcttt tagtattgga agctgtaaga ttgaaaaaag aaggtaaaag catcgaagaa
      601 accctagagt ggctggaaag taattatttg aaagtccatt catgggtaac agtagacgat
      661 ctgaaatatc tagaacgagg cggtaggatt tccaaagcaa gtgctactct tggcagtcta
      721 ttgagtgtaa agcctattat tacggtggat aaagaaggaa ggctgacgaa tgttgacaaa
      781 gttcgaggaa gaaacaagtc gatccagaca atcgtcaatg agacggtaaa aacaatcgtt
      841 gcaccacttg atcaaatcgt ttatgtggct tacgctgggg atctagagag cgcggagaag
      901 gcaaaacggc ttttggaaga gcagataaag atgaaagatg tgaagcttta tcccttagga
      961 ccaacgatcg ccagtcacac aggctatggc tgtatcgcga ttttttcgat gggaatcagt
     1021 cgataaaagc agtaactcat tctgtaaaaa agcccagaaa ttgattgtaa caaaaacagc
     1081 gactcttaaa agatagatga gcatcctatc tcttagaggt cgctgttttt ttatttttta
     1141 agctaaatag gccattatgc ccataactcg ttcaattgtt tttgaatatc tgcattttcc
     1201 aaaaattcat catatgtcgt atcagctcga tcgatcacac ctttatccga aacggcaatg
     1261 atgcggtttg ccaatgtttg gataaattga tggtcatgtg acgcaaataa gattgagcca
     1321 gtaaatgcca tcaagccgtc attcaatgct gtgattgatt ctaagtctaa atggttcgtt
     1381 ggatcatcta atacaagtac gttagctttc gaaagcatca tttttgaaag catgcaacgg
     1441 actttctctc caccagataa aacattcact ggtttcaata cttcttcacc agaaaacaac
     1501 attcttccta agaagcttct taagaaggtg ttatcatctt cttctttact tgcatattga
     1561 cgcaaccagt ctaaaatcgt tagatcagta tcgaattctt tcgttgtatc ctttggtaaa
     1621 taagaattac ttgttgtcac accccaacgt actgttccag tatctggcgt aatttcaccc
     1681 atgataactt tgaatagcgt tgttgttgtt atatcatttt ctgcgacgaa agcgaccttg
     1741 tcttctttgc tcaaattaaa agatatatca tctaaaattt tctttccatc aatcgttaca
     1801 cttacatttt ctacctgcaa taagtcgttt ccaatctcac gttcaggttt gaatccaacg
     1861 aatggataac gtctagaaga aggttggata tcgtctaaag taattttttc aagcattttt
     1921 ttacgtgaag ttgcttgttt tgattttgat gcattcgcac taaagcgggc aataaattct
     1981 tggagttctt tgatttgttc ttcttttttg gcatttgcgt ttgcttgaag tttagccgct
     2041 agttgactag attccaacca aaaatcataa tttcctacat aaagtttgat tttaccaaaa
     2101 tctagatccg ccatatgtgt acatactttg ttcaaaaagt gacggtcatg ggatacaacg
     2161 atcacagtat tttcaaaatt gatcaaaaat tcttccagcc aattgatcga ttggatatcc
     2221 agaccgtttg ttggttcgtc tagcaaaaga acatcaggtt taccaaatag tgtttgtgct
     2281 aataacactt ttaccttttg tccaccagtt aattcactca tttgctgatg gtgcaattct
     2341 tcagggatgt tcagaccttg cagtaaaaca gctgcttctg gttcagcttc ccatccatta
     2401 agctcagcaa attctccttc taattcagct gctcgaatac catcttcgtc agtgaaatcc
     2461 tctttcatgt aaatcgcatc tttttctttc agcacttcat acagacgctt gtgtcccatg
     2521 atgactgttt caagtacagt atagtcttca tagtcgtaat ggttctgttt caatacggcc
     2581 atccgttcat ctggtcccat agtaacggaa cctgtggaag gctgcagatc ccctgataac
     2641 actttaagga acgttgattt acctgcacca tttgcaccaa tcaagccata gcagtttccg
     2701 ggggtaaatt taatattcac atcatcaaat agtttgcggt ctgaaaagtg caaactcaca
     2761 tcatttacag taatcaattt ttttcctcac ttttatcatt attagttttg acaagtctta
     2821 tagaattata taggggaaag gtaaaagatt caaggcaatc gaacattctt tgtgtttcac
     2881 gtgaaacaga atgaaaaaaa taaaaataaa agcggagcga aaatagctac tataggattt
     2941 attagtttgc aacagcataa tttttgacgt tatttataag agggagtact ataaaactgt
     3001 aaaagaaatg aaagaaggat tgaaaatgga tgaaaaaaga accatcgact ggggctcatt
     3061 gattttggga attttatttg tacttgtctc attgttatca tttcaagacc ctgtcggaaa
     3121 tttagttgca attgtagtag tctttgcgat ttttgccttt attaaaggga tatttgaact
     3181 gtttgtacgg aacagattaa aagagctaac aggatataaa ggaaagatgc ccttaataat
     3241 cgggatcatc gatattctag ttggcgtctt tttcttattc aatattggtg caggagtcgc
     3301 agcattgcct tttgtttttg cagtatggtt tattgctgat tcagttttag cgttatttac
     3361 tgctgacttg gcaagaggag taagtgaagg atattattgg tttacgatca tcgtcaatat
     3421 tttaggaatt ttattaggaa ttttcttatt gtttaatcca atttcttctg cgttgacatt
     3481 aagcttttta gttggattct attttatgtt atttggtatc acacatatcg tctatgcatt
     3541 tagataagta aaatcaaaca aataaaaaag ccggcttccg attacagttt tactgttgta
     3601 attggaagcc ggctttttta tcatggacaa atatgcacga ggtgattttt aggtgtcgag
     3661 acaaatttat gcaaaaatgt gagaagctat atacacaaat tttatcttaa tactcgaagc
     3721 atagcgcagt tgtcacaacc tcttttcata taattcattc aatactggca aatcatggac
     3781 ttcatatttt tctttgaatg cttggatttc ttcttcagaa tattttcttg gatctaaatg
     3841 cagatcttct actggcaatg ggcgcttgtt attgatcttc acatcgatta ctacaggacg
     3901 atctgatttt gcagctgcat caaaggcaaa tcgaagttgc gagtattctg tgactgtaaa
     3961 gctatttgcc ccgagcgctt ctccaacttt accgaaatct gcatcttcta aaaatacacc
     4021 aaatttcttt tgttccgtat cttcttgttc tgcttcgatg aaaccaaaag aatcattcga
     4081 aaagacaaca ttaataattg gaagctgata tttgacttgt gtaatgatat cttgcatgac
     4141 catagcaaac ccgccatcac cacttaaggt aaatacttgt ttttctgggt agcttagttg
     4201 tgcggcaatt cctcctggca caccattacc catagttgcg aaccatccag atgtcgtatg
     4261 tttttgttta ccgttcatat tcaataaacg aatagaatgg atagtcgtgt taccgacatc
     4321 tgtgacgaaa atcgcatcgt cttcagcaat acgattgatt tctttataga cagcctctgg
     4381 acgaatcggt tcttcctctc gatcttcaaa agatttcaac caatttaccc atttttcttt
     4441 attttcttga tttgctaggt accagctatc agcagggcga gcatccccta actcgacaag
     4501 tctttgcaaa gctgtgtttg catcaccgag gatcgacaaa gatgtttcat ggcgtcgtcc
     4561 aaattttgac gcatcgatat cgatttggat aaattcagca gttggattaa aaaatgcacg
     4621 tccaaatggg aagtcgctac cgacaaataa gatcagatcg gcttctgcca atgcttcatt
     4681 tccgggttta gtagcgacac gtccagcaaa accaagaaag ttttcataat catcaggaat
     4741 aattcctttt gctagaacgg aagcgacaac aggaactgaa aaatgttctg agaattcttt
     4801 gatttgcgga aatccgtttc gcgttccttg accaacataa agaactggtt tttttgcttt
     4861 ttcaagataa ggcaaagcag ctagcaaatc tttttcttct ggtaatatca cacttgtttt
     4921 gtgagtgtga gcggtggaga aaggttgttc gtcaatttct tcaaaaccaa gatcaacagg
     4981 gatagtcacg atagccacgc ctttatgctc ataggcggct ttaatggctt catctactac
     5041 atgcggaaga ctttgtggtg tcatgactgt acgattgtat acgctaacat ctgcgaaaat
     5101 aggattttca tttagttcct ggaaagagtt atagttcatg gatgtagaag caacttgtcc
     5161 taagaacgct actagaggca catggtccat ctgtgcatca tacaaaccat tgatcaaatg
     5221 tgtcgcacca ggtcctgcag agccgaagac agcgccgact ttccccgtta attttgcatc
     5281 tgcagcggct gcgagtgcgc cgacttcttc atgacgaact tgaatgtagt tgatttcggt
     5341 tttttcttga tatagcgcat ccataattga attgaaagat cctccgggaa tgccgtaaat
     5401 atggtcgata ccccagcttt caagaacttt taccattgcg attcctgcat tgatttttga
     5461 catggtgttt cactccttta gcaatgatga tacataaatt ttttgtatct agagtataga
     5521 actttttatc gaaaatccca actaattcgt cttttcacaa aaatgtagtc atgtttttgg
     5581 gaatatgcta gagtagtaca tagatagatt caagagaggg gaatggcatg gttgaatggc
     5641 tattgagact tgaaccttgg cagcaagcac ttgctgggac agcttttact tattttatga
     5701 cagctcttgg tgccgggttg gtttttttct ttaaagaaat caaaaaagaa gtgctaaact
     5761 tgatgttagg atttgcttct ggtgtcatga ttgctgcaag tttttggtct ttgttagatc
     5821 cggctattac aaaagcagaa gaaaatggag atattgcttg gctagttgta agtattgggt
     5881 ttggtttagg cggtgtgttt ttatatatgg cagataaaac actgccccat atgcattttg
     5941 gaccacaaca tgaagcagaa ggattgccta cccatttaaa acggacgatc ttgcttgttt
     6001 tttctattac attgcataac attcctgaag gtttagccgt gggggtagca ttcggtgcag
     6061 cagcaactgc ggataatccg acagccgcta tattagccgc tgtttcggtg gcgctaggta
     6121 ttggtatcca aaacttccca gaaggagcag ctgtatcaat cccgttgcga caagaaggtc
     6181 tgagtcgtaa aaaggccttt gtttacggac aagcttctgg aatcgttgaa ccaattgctg
     6241 gagtgattgg agcgctacta gtaacgaaag tggagctgct gcttccttat gcacttgctt
     6301 ttgccgcagg agcaatgatt tatgtcgtgg tggaagaatt gatccctgaa gcacagcaga
     6361 cattgtccag caaacgtcat tttgcagttt ttggcgtcat gagtggattt atcattatga
     6421 tgattttgga tgtggcatta ggataaataa aaaaaatata tatgatatat caaatagggc
     6481 cgcgaactta tatcgcgacc ctattttttt agtaagttat atcataatga agtgaatggt
     6541 atcatacatt aataatagtt ttttctatgt gtatttcctt tgatccagcg aacaagccag
     6601 atgataagtg cgattgggat tagaactttg accgcaaacc ataaaagccc gcttaggaca
     6661 ctgacaaaaa gactgatgac gaagctagct gcaattagca ctaaaagaac catgaagata
     6721 tttaagaaca tatttttttg acgcatacaa aaccctcctc ttgttcattc tttactatag
     6781 aaaaagtata acagggattg tttaaaaaca cctccaactg aagactgatt tttacttcat
     6841 ctacttaaat gatcaaacaa ttgctttttc tctggtttat catctaaaat agagcggaaa
     6901 aggaggagaa tatcatgcct aaaaatcgtc ttgaagcatt tacagatgct gtaatcgcga
     6961 ttatcatgac tattttagtt ttagaacttc atgaacctaa cggagacacg tgggaggcgc
     7021 tcgctgcatt aggttaccga ttttttatct atattgtcag ttttatcatt ttagcgattt
     7081 attggaacaa tcatcatcat ttatttcaag ttgtacataa gataaatgga cgggtcttat
     7141 gggcgaacag tttctttgtt tttgcgcttt cgctttttcc gtttgcaact tcatggatca
     7201 gtgagtacat caatagcctg gctccagaaa tgacttatgg ttttgttacc ttgaccgcta
     7261 atattgcgta ttatatcttg gcaaaagaac ttgtcaaagc aaataaaggc cacgagaaag
     7321 cattgaaggc gatgttgaat aattacaaga aatcgtatct atcgattggt ttgaatgtgt
     7381 taggaattgt cttagggtat ttagtagcgc cagtagccgt gctgatcgca aacatcgtga
     7441 ttctagctgc ttggatcatc ccaagtcgta ccatcgaatc acaatatcgg taaaatgatc
     7501 cgtgtttaaa atttaagaga aaaatataga aaaaataaat aaacgatcaa atatccgagt
     7561 gatactagta ttcaaagagg gatgcagatg gatcaaaaaa gaattcatgt aggactgatt
     7621 actagttttg tcacgatgct tatttatttg gatcaaggtc atttgacttg gacagtagtt
     7681 ggtgtcgtag gatttttgac attattcaca gcaataaaaa aataaacatc tttatgtaat
     7741 ctgatgttct atctatttaa gaaaagcatg tttacagcag tatgattctg ttgtaaacgt
     7801 gtttttattt gtggaaaatt taatttttag aaaagtgtat cgaaaaatag atagttagtt
     7861 tttccacagt ggataacttc gtaagagata aaaataaaaa attagtcaaa gaagggcgta
     7921 tagatttctt ttgataggag atatacaaca gaaatgaata aatattaacg tgttttcaaa
     7981 cagtataaaa cggtgtggtt tttggaataa aaaaataaca tatctcattt tacacttttg
     8041 ttattttata cttaaaaaat aacgacacag aaaataccgt gtctcagatt tctctactac
     8101 ttctaaaaac tactgttttt ttcctttgaa atgccttcaa aaagtaggat caggcagaat
     8161 atactggcag aatcaaggag aagggagcct aaataaggag aaaaaccata gaaaatataa
     8221 agtaattact tataaaacaa gctttttata gaaaaataat atacctgagt agggtataaa
     8281 ttaagacgga attcctttct agattgagat attgactagg aagaatgtta agattttaag
     8341 cctttaagct aaaaaaaaac cctttttgtt aaataagggg aatatagcct gtcagtttca
     8401 aactgcaagt cgtcctgctg caataatata gtagattaga gatgaaaata gattttttta
     8461 aaccaatgac tgaataaaaa gaccgtcata gatacacatt ggaatgtgtc tattacggtc
     8521 tttttttacg aacgatttta ttgaagacgg acagttcggg tcaaaaataa aaaagctacg
     8581 atcagaaata agaataaaat gtatcccaaa ataatggcta gacggtctaa cttatttgtc
     8641 gatagatgtt tgtttagaag ttgataggcc cataaaccta tcagtcctcc tgttgtatta
     8701 gttatcaaat cagtgatgtc cgctacgcca ataccgaata ggaattgaat caattctgcc
     8761 aagaagctta caaggaaaat aatccctaat tttttagaaa aagaaagtct cttgaaattg
     8821 acattcaaaa gcaccccaaa aggaaaaaag acaatcaagt tataagtggt ctcacgtaat
     8881 accgtcgtat tttcaaaaga aaaagtagaa aaagggacga gattgacatt gctgtaagaa
     8941 taagtgagaa attgtggaag attaaatgat agcttgaata aaatcaacca taatagaaag
     9001 aataaataaa tccctaataa aaattttgat acataccgca tagacatctc ctccatttaa
     9061 aaagtatacc atcgaaaacc gctgaaaaat cataaaaaag tatgaagttt aaggaccaac
     9121 gtagttatcg tacgaaaatt agaagctttt ttttgttggg aatccggttt ctttaacaaa
     9181 gctaagatag cgagttgctt ggaaaagttg ctatcttttt tctgtcaaaa cgactcggaa
     9241 tgttcgtttt ttgttctaat ttctccaagt tgtttacttt ttgatactaa gtttatgaaa
     9301 aatcaagaaa tagttgttat actaggtaat gctgacaaac gggattggca tttcatcttg
     9361 gttcagcttt ttttccaatt actaggaagg agggatagat tgttcaagac ttattttcgt
     9421 ttcaataaaa tacaagataa tccttttcgt atcatgaatc tgatgctact tatctttatt
     9481 gtattatggt taggttacat aaatcatgat tttttgattt caagtttttc atcattaggg
     9541 atctttactt ttttctatta tcaaaacata ccgatgaaat atttgctgaa acgtatgttt
     9601 ctgatcggtt gcggactgtt ggttgctttc gttttgggga tcttatcgac ttatgtttta
     9661 tggcttgaac catttgccgt agctttggtt gctttttcca gcagattcgt gttgcgatta
     9721 ttccatattt ctaaaccagg tggcttgttt tttgcgatgc tttctgccat gggaacgagc
     9781 atgcagctgc caatcgctca attaccgatc gtctcacttt atttttttat gggtgtcgtt
     9841 tttgccctga ttgcggcagt gattacaaaa ttactggatt cacggcctga gcagacaatt
     9901 gaaaaagcaa ctttaaaaga aagatttcac gaagagcctt tagtgatcat tgattctgtt
     9961 ttctacagcg ctgccttatt tctttccgtt tatgtcagtc acggcttgaa tctgcataat
    10021 ccttattggt tgaccttgtc atgtgcatcg attttgctag cagaaaactt ggatgcgatg
    10081 aagcatcgac aagtccaata tctgattgga tcgatgggtg ggttatgtgt gtcggcattc
    10141 ttgtcatttg tgccgtttac gcagttgcag accatttttc tgatcacttt tctatatgga
    10201 atcgcacaat ttctagttgc tagaaattat gcagttgcta atatttttct taatccgatg
    10261 gcgttgatgc tatctacact tattcgtgga gcatacttga tttctttgat cgaatatcgt
    10321 tttttaggga tcgtcatagg aagtttcatc ggtttaggtg ttgcgtgggt aatgacggtt
    10381 ggtttgcaac attatttaac ggttgttcgt caaaaattag aatagaagga caaatatatg
    10441 tgccaaaaga ggttatgaca agatttttgt cacaacctct aattcaaggt gttcaaaagc
    10501 cagattctca gcattaagtc aaattcacac aaaaatgtgg aaaggaactt tcaaaaaaat
    10561 tttatattta ttctatcaaa aaattttgtt tatgatggta actgaataaa ctttcctgag
    10621 atggtcaatg ctttccttga aagtcttaca gatattcttt cttcatagaa agtttaagtt
    10681 ttatagttag tgtacaataa aagtatgttt ataccatatg gtgtccggat aatcagtcat
    10741 ttaaacaatg aggttcaaag atatgaaaag gggaaatttg atgaagaaaa taagcaatga
    10801 aatccaaatt ctggcagaaa ctttaactct tcccaaagtc gttatagagc aaatgattgt
    10861 acaagcaaaa aacatccttg atgaggtgtt tgagcaatat agtcatgaac taatggatcc
    10921 taataaagca aaggcgactg caaagaaaat agacaaagtc tatgaagagg aaccttttgc
    10981 agcattagct atttatctat atgcagcttc tcaaagttgg aaaaaaattt atcaaccatt
    11041 gaagattcca agaactatct atttggcaac aatgaatgct tttactagat ttattcatga
    11101 acatttccaa gtaacaggaa gctatcgctt tgaccgtggt ttttggattt ggcattacac
    11161 tagtggcttg atctttcgga ttggtgaatt ggagtacgaa atgacatatt ttccgaaagg
    11221 gtacacggat ctaagattag aaggaaagca gtgcctttct cttcatattc cttccgatgc
    11281 agatttatct cagaataaga taagagatag ttacgaaagt gccattcgtt tttttgaact
    11341 ttattttcct gattaccatt accaagtgat gtacacagat acttggttac tatcacccaa
    11401 tcttactaaa tggttgaaaa aagagtcaaa aatctgtctt tttgcagccg attatcgatt
    11461 attatcagtt gatgaacagg acgatagtgg agtcccttgg atctttggac gagtagatgc
    11521 ccaaatacat gattatccag aaagtacgag tttacagcga caagcaaaag agcaattgct
    11581 tgcaggcgag cacattggct ctggcttagg tattcttact tcataagttt agtacttcga
    11641 aatagtttaa aagagcgtga gacaaaagta aagaacactt ttgtctcacg ctctaaacac
    11701 gtataaacgg cggaagcaga agcaactcct tcggaaataa gctgaaattc acaaaaattt
    11761 gaaaagcaat tttcgtgaat ttcttcttat ttcttggagt taaacgcttc tgtcccagcc
    11821 tcttttttag tgttctatcg aacacacagt acgttcaata ggtaaactag ctataacaat
    11881 agatattaaa gttaacggac aagagggcct tttttaggta gctaaaacgt aataatgaaa
    11941 atttatttcc agagatagga gtcaatcagc tagtctgatc gactgtttta acaagttgac
    12001 tttcatttta acttttgata ccatttaaaa cacaaatcgt aattgtttcg atttgtgttt
    12061 tttagcatga ctacaatcaa agaagaaagt gggtcgtcca tatggaaaaa acagatatct
    12121 caagtgcata ccgccgcttg aaaagcccaa atatcaaaac acgaaaacgt gcgctgaaga
    12181 tcatcaaaga tgtgaaacgg aatagtggga aaaggtaaga agaaggaaac ttaattaaaa
    12241 aaatagccga aaaacgtttt tcggctattt tcaataatcg gtttttgtgt aaggtttccg
    12301 aaagaactgc tgttgatttt ccatttttaa catatgataa attggtagaa tctttctttt
    12361 caaattgata ccattcccta attccagtta tatagttaac tacagaacca caaagcacgt
    12421 acgtttataa aagtattatc tgtagttatt tttcaattcc ctttcaacct cttattttga
    12481 ccagtagtat tgatcacttt accttttaac acctttgttc gtttttgaaa catttttagt
    12541 caagaatgga aacgtaacta cataatcaaa ggttttgcga aatatttcat gaaatatttc
    12601 atgtagaagc gagcaatacg gttgcaaata accattatcg tttaataagc agttacgaaa
    12661 gctcccaata tctctaacgt tcagctcact ccatttcttt tcttcaaaaa aatcagtgat
    12721 tcgtatctct ttctatttgt cgaaaaagtt aactttaaaa agtaacgccg aaaaaatatg
    12781 caaaaaaata aaaaagcagt tactattgct agaataacat caaggaataa taagttttgg
    12841 ttttcagata atagtcgata agttgtgtat tggaatttta gatacagtaa taaagtattt
    12901 ttcatgaaat tttgtgagaa tcactattac aagtatcttt aataaaatta atggttaagg
    12961 aggaacttaa agtagaatgt aaatgaatag ttgtagtcag tcgattgtga tactagactc
    13021 aaagttgcag gaataagttt ttcaatttgt gattggaaga taaaaaatga aaaagaaggg
    13081 ctttttttta tactttgttg attgtagtgt ttattttccc tctgtttagc caagtaacat
    13141 tttctatgac tcaaccagtt tcaagtgagg aacatgataa cctttttcgt gctacagaag
    13201 agtttaagaa atatcaggaa aatattgttc cggagactcc attagctagt gagccaatta
    13261 ctgatattga tggaaaccaa ataggatggt atatacagta cgaagttatg gtggtaaata
    13321 atgactcaat agtaaaaaat acagtaggct tagagctaac accgattgaa aaggtgagtt
    13381 cattgttaac gtatatttat actgatgaaa gtgacgagac tgagatcatt ttaacggatt
    13441 actcaaaaat tgctactgat aaaaagtata tattgtggat gtagacacta gtgaaatact
    13501 ttatgatata gaaattgatg aaaatttact agatgtagca caagaaatcg atcatcaaaa
    13561 gcaggaagct attcgatatg ctggtagaaa agggaaaaga aaatatttat tacgtgaatg
    13621 tagcaaaagt tagagaagat gaaaatgagt ggaaagaatt caagagtcgc tactcaatta
    13681 actcgactcc gacatttaca gtttacagag aaggatcgat agaaaaaact gttttttgga
    13741 ctaaagaaag tggaatgtct ttagctgaag tagaagagtt tttggattat gttagtatgc
    13801 aacagtaagg gagttcttat tatgaaaaat tttgacacat attatgttat tgttttttcg
    13861 atatctttgc tgttaatatt actaacttta atgcagtata aagagtatcc agagtatttt
    13921 attttaactt ttgtatattt agtaagtagc tacacacttc tatataaatt cagaagttct
    13981 tctagagttt ctaaagtttg tttcttaatt agcttattga ttctataata tttttgattt
    14041 atataggaaa ttaaaaatat tatagaatca acactcagag aacgggttgg tttttaatag
    14101 atttaataag tactgataat gaaatttctc ctggttggga aaacgaagtt ggttaccctg
    14161 tagaagaatt ttttcaagat tactttgcgg agtatgccga taggataatg agagattacg
    14221 taacagataa aactcaagaa gtcatcaatc aatcccagtc attaaatgaa ttatctgata
    14281 aggcgaaaga agaagcagtt gccattattt cttcagggat tagtgactat attgatatga
    14341 gtggtgaaat caattatggc gataaagaca aaattgtacg catgataaaa gcttttatca
    14401 aaacttttga tgtgaaggca gatcttaagt ttaatgatac ttacctagta ggaaaattaa
    14461 ttaatatcct tgggacagag aaagatacgg aaacgtttat ttcctctttg tctatgttgt
    14521 ttacagataa gcatttttca actcagcttg gtggagagga actagtggaa atcttttaac
    14581 cattacgccc agcattaata aaagtatatc aaaaaagtac tcctgatgat ttttaagatt
    14641 ggtttgagaa ataatgtgaa aacactattt ttaatgttaa ttatagatag agatgaacgc
    14701 gtcatatggt taaaagaatg aaaaagtcaa agatacactg ctatatttca ctaaggatat
    14761 agcagtgtat ctttcatgtc gtttcactgg cagtttttta taaaaggagg tgtgcatttc
    14821 agttgctttt ctttggtagt tagttgaatc gtttaagaat taacggatgt ggcagacagg
    14881 tgctctgtcc aaaaaccagt aaaatctttg atggttccat ctttatttag ctgtgcctgt
    14941 tcaaatactg ttttgttttt accgaatgga tcaattgcaa taattgttcc gatccccgca
    15001 cgtttggcag caagtaatcc tgagtaagca tcttcgataa caagacaatc agccggagct
    15061 aagctcagtt tgtctgccgc tttaagaaaa atatccggtt gcggtttccc agggaaagag
    15121 ccgtcgtcat agacaatctt gtcccagtca aaccagcggg ctaaatcgaa aatatcaaaa
    15181 taaaaagcaa cattctcttt gacagtagcc gtggcaatcg ttaatggaat ctgttggtca
    15241 gtcagctggt caagagtcgc ttcaagccct tttgtcaact gaagctcgtt ttcattttgc
    15301 aagcaaagca tacgataata gctttctttt tcttctgaca atacggcgat ctcagaggcg
    15361 gtcaaatgct ctgaaatgaa atgtttcaaa atctccgtat tcgtccgacc atgaatattt
    15421 tttaaaatat cctcgtctga taaaccatct tttgtatgat catgtatcat atgcagccaa
    15481 gcagcttcat gcagatgaga gtccaaaaac attgttccgt tgaaatcaaa tataatccct
    15541 ttcatagtta cgctcctata gtggtatttt cggttgaatc agcaatttgc agatcgacat
    15601 attgtgcata gtgctcttga acctcccgcg agatctggcg atccgtaatg agcagatcga
    15661 aatcatgcaa attataaaac acataaaaat catctcgatt gaatttttta ttatcaacga
    15721 gtagatattt cttacgggaa ttatttagag cgatctgctg tgcttctcct tcttcggtac
    15781 tgtaagtaga gatcgcttcg ttgtgaacgc catttgaact gataaatgct tttgtaaact
    15841 ttagtcttct taaattgtcg ttggtgatcg gtccgacaaa tgcgccggta tttttccgga
    15901 aatcaccgcc aatcagtaga atttctggtc catctgcttg agcaagaata tcaaaaactg
    15961 gataattatt tgtaatgatc cgaattttct tgttcagtaa atgcgtcgcc aatagttcga
    16021 ttgttgtacc tggtcctaag aaaatcgttt ctccctcgtg aatcaaggag gctgctgtgg
    16081 cagcaatttc ttttttttct tgaatctgca gagtggcttt ttcagtgtga gaaagttctt
    16141 gatctaaagt gtaggaaaga ctttgagccc ctccatggac acgcagcagc ttgcccgatt
    16201 tttctaattc atctaaatct ctgcgaacgg tcatatcaga aacatctaat tgattgatga
    16261 tttcattaac ggtaaggatt ccttttttat tgaccatttc tacgatagta agcaatcttt
    16321 cttttttcaa cattgtcttc acctctaatt taaggataat ctatcatata aacggacaac
    16381 agtcaatcta attcgaacaa aagtaaacat ttagggtggt ttttatgttt gttacaatag
    16441 attttattgc tgttttctgg tttttatgat tatttgtgtt tgtttttgtt gacaaatgtt
    16501 ttggatgcgt ttacaatgca atcagttaga aagagcgaaa actgattata gatttgagga
    16561 gagatgtaca tgcttaaatt accagaagat tttatttttg gcggagccac agcagcctat
    16621 caggttgaag gagccactaa ggaaggtggc aaaggcgctg ttgcttggga tgacttttta
    16681 gaggaacaag ggcgattcag tcccgatccg gcaagtgatt tttatcatca atatgcaaag
    16741 gacattgaat tgtgtgaacg ttttggtgta aatggtctaa gattatccat tgcttggagc
    16801 cgcatttttc ctgatggagc tgggaaacca aatccagaag gaatcgcttt ttatcatcgt
    16861 gtgttcgaag aatgtaagaa aagaaacgta acgccgtttg tgacgttgca tcattttgat
    16921 acccctaaac gtttgtttga ccatggagat tttttgaatc gagaaacaat agaagcattt
    16981 gtttcctatg ccattttttg ttttcatgaa ttcaaagaag tcaaggtgtg gagtaccttt
    17041 aatgaaatct atccagttgc aaccaatcaa tatttgttag gtgttttccc accaggaatc
    17101 aaatatgatt ttacaaagat cgtcgcctgt cttcataata tgatggttgc tcatgcccgt
    17161 gtagtgaact atttcaaaga aaacgaactt ccgggtgaaa ttggtgtagt tcactcatta
    17221 gagacgaaat atgcggcaac agacgcgccg gaagataagc atgctgcctt tttagacgat
    17281 gcattgtcga tccgtttctt attagatgct acctatttag gctattattc aactgaaacc
    17341 ttgactgctc ttgatgagat atgtgaagca aatcaggcca gctatcattt tcctgaagaa
    17401 gatttcgttg aattgaaaaa agcatccacg cgtaatgact acttagggat caatcactac
    17461 caatgccatt ttgtcaaagc ttacgatgga gaaaatgcaa tccatcataa cggtacgggt
    17521 gaaaaaggga cctctgttta taaagtgaag ggaatcggag aacgtattta taaagaagga
    17581 attcctcgaa ctgattggga ctggctgatt tatccagaag gtctatatga cttgttgctg
    17641 cgaattaaaa gcgattaccc acattacaac aaaatctata tcactgagaa cgggatgggg
    17701 tataaagatc agtttgaaga tggcattatt atggatcagc cgagaatcga ctatttgaga
    17761 gtctatttgg aatccttaag caaggcgata acagccgggg taaatgtgaa ggggtatttt
    17821 ttgtggtcgc ttatggatct tttctcatgg acaaatgggt ataacaaaag gtatggcttg
    17881 ttttatgttg attttgaaac ccagaagcgt tacccaaaag aatcagctta ctggtacaag
    17941 ttggtcagtg aaaccaaaac gattatttaa taagtgataa tggaggaaga aaagatgaat
    18001 aaagaggaat tacaaatgct tggttttgag atcgttgcat attcaggcga cgctcgcagt
    18061 acattattga aattattgaa agaagtaaga caaggaaact ttgaacatgt tgattcggcg
    18121 ttgaaagaag ccgacgaaaa tttaaccctt gcacataatt cgcaaacaaa aattttagcg
    18181 gaagaagcat ctgggaaaga gatggagatg ggattcattt ttatccatgg gcaggatcat
    18241 ttaatgacca ctttattgct gagagatctg attcaagact ttattgtact gtacagacag
    18301 aatcaaggat aggagaagaa cgctatggat ggaatcatta aacaaatcga aaaagggaaa
    18361 ccattttttg aaaaaatctc acgtaatagt tatttaggag ccgttcgtga cggattttta
    18421 acagcgatgc cagctatttt attttcaagc attttcattt tgattgctgc ggttcctgag
    18481 atttttggtt accaatggcc tgaatctgtc tccacatggc tatggaaagt ctataattat
    18541 tcgatgggtg ttgtagggtt actcgtttca gcaaccactg ctcgctgctt ggctgattcg
    18601 atgaatcgtc gcttaccgaa aaacaaagta atcaacacca cctctgtgat gctggcctct
    18661 gtggtaagtt tcctgttgtt aagcgtctct ccgattgaag gtggattttc aacggaatat
    18721 atggggacaa agggactact ggcttccttt gtcgctgcat ttttaacagt taatttatat
    18781 aagttttgtg tgctgaaaga tattaccatt aaaatgccta aagaagttcc tggaacgatt
    18841 tcgcaaacct tccgagatat ttttcctttc tctttttcag tatttgcggc cgttttaatt
    18901 gatttaggca ttcgacaaat cttccaaatc tcatttgcag aagcaatcat tacggtgctg
    18961 cagccgctat ttacagcagc tgacgggtac ttaggaatcg ctattatctg gggagcgatg
    19021 gcgctgtttt ggtttgtcgg tgttcatgga ccgtcgatcg tggagccggc aattgcggca
    19081 attatctatg ccaatgtgga aaccaatctg caattgttca attccggtga acaagcttct
    19141 aaagtactga cggtgggttt agggaatttt gtaggaacta tgggaggaac tggggcaaca
    19201 ttggttgttc cttttatctt cctactcttt gctaagtcta agcagttgaa ggcagttggg
    19261 aaagcttcct ttattccagt aagtttcgct gtcaacgaac cattgctatt tgcggcaccc
    19321 atgattttga acccttattt ttttattcca tttttgttaa cacctgttgt aaacgtttgc
    19381 ctgttcaagt tttttgtcga tgtattgggc atgaacagct ttatgtacgt tttgccttgg
    19441 gcaactccag caccgatcgg tttggttcta ggaacgggca tgggtatctt agcttttgtt
    19501 ctagcagcgg ttctaatcgt cgttgacttc ctgatctatc taccattttg caaagcatac
    19561 gatgctgtct tagtagagaa agaagcacaa aaagcagagg tgatcgcaac cgaggaaggt
    19621 gcttctgccg atgtacgtga ggaagtacct gtggcagcga gtcaagttat cagcgatgca
    19681 aataaagaaa tcaatgtgtt ggtactttgt gctggcgcag ggacaagtgc gatgttagct
    19741 aatgctttaa aaaaaggtgc atctgaatcg aaagcaccga tcagtgcaat ggcgggtgct
    19801 tatggttctc attacgaaat gctgcaggat tttgatatgg tggtcttagc accgcaagtc
    19861 aattcttact atgatgatat caaagaagac accgatcgct taggaatcaa gctggttgcg
    19921 acaaaaggag cagaatatat cggattaacg agagatcctg aaaaagctgt tcagtttatt
    19981 ctgaaccaat ttcaataaag gaggagccat aatgaaaatg ggaatttggt tgcgtaaagc
    20041 tgttttattt gcagctgtgt taggggcttt gggggctgtt tcaaaaactg ttgaagcatc
    20101 tgccagcgaa tttattcgcg gagcagatat ttcgatcctg tcagatatgg aaaaatctgg
    20161 tgcaaaatat tatgaagacg atatttcaaa agatgcctta gagattttaa aaaataatgg
    20221 tgtcaactat gttcgactgc gtttgtggca ggatccttat aatagtaatg gagaaagtta
    20281 tggagcaggg acaaatgatc tggaaacgac gattgacctt gcaaaacgag caaaaaatct
    20341 gggacttaag gtgctgctgg attttcacta cagtgatttt tgggttgatc ctgggaaaca
    20401 gaatcttcct aaagcatggc aaggtctgac ctttgaagaa atgaatacgg cattgtacga
    20461 ctatacaaag aacgttctgt cggaaatgaa gcagctggac gtttatcctg atatggttca
    20521 gatcggcaat gaactgaata gcggaatgct ctggccttat ggcaagtctt ggggagaagg
    20581 cgggggagaa tttgatcgat tggctgcttt tttgaagtct ggtatccaag ctgttcgaga
    20641 cactcaacct aaaacaactc cagtaatgct ccacttagct gatggtggcg atacaggagc
    20701 atttacttgg tggtttgatg aaatcacgag tcgaggtgtg tcatttgatt taattggagt
    20761 gtcgtattat ccgtattggc acggttcaat gaatgaactt caaaccaata tgaatgcgat
    20821 cagccaacgg tatggtaaag aagtgatcgt agttgaaaca gcttatgggc atacaacagc
    20881 gaatgccgat actatgccaa atgcttttgg tgaagccgaa gcagctgcgg ggggatatga
    20941 accaacaccg acaggacaag cagagtattt gctggatctt gccgatcgga ttcaggcagt
    21001 accgaataat cgcggggcgg gcttcttcta ttgggaacct ctttggtaca acggaaatgt
    21061 gagttgggca acacaagcag gtatgaatta tctcagtgtt caatccacta tgggaaacga
    21121 atgggacaac caagcgatgt ttgattttgc tggaaacgcc ttgccaagct tgcgtgcgtt
    21181 taaacaagca ggggcgcaaa taaatctagt taaaaatgca agtttcgaaa gcgatggacc
    21241 aacgaccagt ccttccaatt ggcaaacctg gtttcaacaa ggaaccgcta agggtacgat
    21301 caaaacggaa gcgggcactt tttttgggga ctacaaacta actttttggc aggatcaagc
    21361 atacgaggca tcagcttatc aaaatgttag tggcataagc aaaggaactt acacattgag
    21421 tgcctatgta atgtctgggg gagagtttga tactaatcag ttatatatca aaaattatgg
    21481 tggagcggaa ctaaatactg cggttgttaa aacaagtgaa tggacaaaaa ttacaatcaa
    21541 agacattcca attacaacag gtgtttgtga aattggggtt tatagcaagg cttcaggaaa
    21601 tgcttggacg aacgttgatt ttgtttcatt ggtgaaacaa tagactgcgg atcgtttcga
    21661 ttcaagatcg gaggttccct tcagccacat cgttagcgta gaaggcagga atcgaacctg
    21721 cacctcgcct ttgccaaaaa taaccagaaa cgatttccag tcatttttac caaaacgagt
    21781 aaagcaccag acttcatcta cactttgtat tatgctaaag agagttcaac gatttcgtaa
    21841 gaagcaatga aaaaattgca aaaagttgtg cagataacat aaagtttgtt taagaaaggg
    21901 taaccttaga attccgtcgt gaatatgatt tgagcactta gatggataga aaaaagttct
    21961 cggaaaacag ttcgtttttc gagaacttct ttttatgata agaatgaaac aaattaactt
    22021 atattatagt ctcttttggt agggaaccat cttattttta atgaaaatca agcattttag
    22081 ctattgaaga atgttgattt atagggattc cccctccata ttatgttggc tctttattcc
    22141 cactcaatat gagtagcctc gtaaagaagc tattatatca gtgctttttc agcctattcc
    22201 tcattttggt agggaaatgg tagggttaga tttttactgc ttgatttcca ttaaagtttg
    22261 caactttaga ttttgttgca caggtaaatt taatgtttcc tgtcatttgt tctgcaattc
    22321 cttcatcatt acggctccat agatgagcgt agtgctttaa agtgatttca gggctagaat
    22381 ggcctaaccg ttgcgacacg accagaatgt ctgcgttaaa ctcattgatt aggtagctaa
    22441 catgagaatg acgtaatcct tttccttgta ttaggggtac cttagctagt ttggcataac
    22501 gctgaataat tcgttgaaca gttgaacgat agagtgggag gtcagtgtag ctaagaataa
    22561 attgttctac accatgattt ttttgaataa ctttccactt ttttagcact tcaattgtat
    22621 catcgtccag agaaatgatc cgcaagccgc tttcagtttt tgtgtaaggt ttccgaataa
    22681 aatctttttg atttgtcatt tccaaggtgt gatgaaccct caacttttta cgtttgaagt
    22741 ccacgtcatt ccaattaagt gctaatcctt cgcttacacg aacaccagtc atgtaatata
    22801 gccagatcat taaaaaacac atatgttcgt agaaatcatc tttataaatt tttgccaaaa
    22861 cttcttcaaa ttcttctttt gtccagtaag gaacgattga ttttcctttt ggaattgctt
    22921 ttgttctttt ggaaatattc tcattcagaa attgcagggt gaccgcatag tctaatgatt
    22981 tacgaaacat accgtacata agtgaactgt atgcttgtga gtatccgcta tcgttcaata
    23041 accaaattcg ataattttca caatctcgca cattgatttc acgaagcttc ttatcaccga
    23101 aaaattcagt aatctgaatc aatccacact cacgagaact ccaagtactt ttttgtacgc
    23161 tagctttgta gtgtggaacg taagactgct ccataaattc tttgtaagtt aaatcataat
    23221 tcgcaaatcc atttacaacc atgtattcat tttttatacg catgacttct ttgtgtgctt
    23281 cacgagctga tgagaatcgt tttcctagag aatcttttgt tcctttcttc tggatcctct
    23341 ttccagtcac ttgatctgtt cctaactcaa ctagataaga aaagcgacca tttttatctt
    23401 gatacacaca agggtatttt gttttcatag ttattcctcc tgtgaaagag gaacacctat
    23461 aatttcttca attacttgtc ggggtactac cccaactctt ttattattat aatatggata
    23521 tccctgattt accattcttg tttttgcttg tcggattatt gtggtagctt gatgtgtttt
    23581 aaatcctaga gcctctaaat cttttcgaca aattgtattt ttcattgtat aaactcactt
    23641 tcaaagaaaa cactatcatt gggtaatagt gttatttctt tatttttgta taatgaacgg
    23701 tactttcttg aaaatccatc acttgttcag gacttcccaa ctcatttaac agaattaggc
    23761 agaagtcgcc catcctctat aggtgggtga tgaatgccgt tcggtatgag ggttaccgtt
    23821 ggtaactcga agaccgacaa ctaaggcgta ttgccttttt attttgt
//
LOCUS       DACDYI010000050        23828 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.050, whole genome shotgun sequence.
ACCESSION   DACDYI010000050 DACDYI010000000
VERSION     DACDYI010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.050"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(53..787)
                     /locus_tag="IWM52_001846"
     CDS             complement(53..787)
                     /locus_tag="IWM52_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP9974093.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="IWM52_001847"
     CDS             1114..1524
                     /locus_tag="IWM52_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAP9974094.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="IWM52_001848"
     CDS             1517..2218
                     /locus_tag="IWM52_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAP9974095.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="IWM52_001849"
     CDS             2218..3804
                     /locus_tag="IWM52_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9974096.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="IWM52_001850"
     CDS             3823..4044
                     /locus_tag="IWM52_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAP9974097.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="IWM52_001851"
     CDS             4041..4802
                     /locus_tag="IWM52_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAP9974098.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="IWM52_001852"
     CDS             5182..5691
                     /locus_tag="IWM52_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP9974099.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="IWM52_001853"
     CDS             complement(5766..6305)
                     /locus_tag="IWM52_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAP9974100.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="IWM52_001854"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="IWM52_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAP9974101.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7372..8169
                     /locus_tag="IWM52_001855"
     CDS             7372..8169
                     /locus_tag="IWM52_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAP9974102.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8197..8832
                     /locus_tag="IWM52_001856"
     CDS             8197..8832
                     /locus_tag="IWM52_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAP9974103.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="IWM52_001857"
     CDS             8852..9625
                     /locus_tag="IWM52_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9974104.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="IWM52_001858"
     CDS             10033..10830
                     /locus_tag="IWM52_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAP9974105.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="IWM52_001859"
     CDS             10808..11221
                     /locus_tag="IWM52_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974106.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="IWM52_001860"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="IWM52_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP9974107.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="IWM52_001861"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="IWM52_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAP9974108.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /locus_tag="IWM52_001862"
     CDS             15998..16558
                     /locus_tag="IWM52_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAP9974109.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="IWM52_001863"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="IWM52_001863"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAP9974110.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="IWM52_001864"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="IWM52_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAP9974111.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="IWM52_001865"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="IWM52_001865"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAP9974112.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="IWM52_001866"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="IWM52_001866"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAP9974113.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="IWM52_001867"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="IWM52_001867"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAP9974114.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="IWM52_001868"
     CDS             21905..22483
                     /locus_tag="IWM52_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP9974115.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="IWM52_001869"
     CDS             22535..23017
                     /locus_tag="IWM52_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAP9974116.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="IWM52_001870"
     CDS             23134..23508
                     /locus_tag="IWM52_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974117.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7781 a         4285 c         5116 g         6646 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaga
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcaccgggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggaat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atggaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgat gaaaataaaa taacaaacaa tttgtaaagt taacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcatagcatg acagcattcg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ttagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaagttt
//
LOCUS       DACDYI010000051        23720 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.051, whole genome shotgun sequence.
ACCESSION   DACDYI010000051 DACDYI010000000
VERSION     DACDYI010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.051"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..167
                     /locus_tag="IWM52_001871"
     CDS             <1..167
                     /locus_tag="IWM52_001871"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="catalyzes the removal of N-terminal amino acids
                     from peptides and arylamides; generally Co(II) however
                     activity has been shown for some methionine
                     aminopeptidases with Zn, Fe, or Mn; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAP9974118.1"
                     /translation="EPMVNTGTWKMKMDPNGWTAYTRDGGLSCQYEHTLAITKDGPRI
                     LTSQGEEGTY"
     gene            199..1122
                     /locus_tag="IWM52_001872"
     CDS             199..1122
                     /locus_tag="IWM52_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAP9974119.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            1198..2337
                     /locus_tag="IWM52_001873"
     CDS             1198..2337
                     /locus_tag="IWM52_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAP9974120.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            2330..3130
                     /locus_tag="IWM52_001874"
     CDS             2330..3130
                     /locus_tag="IWM52_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9974121.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            3130..3957
                     /locus_tag="IWM52_001875"
     CDS             3130..3957
                     /locus_tag="IWM52_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9974122.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            3935..4669
                     /locus_tag="IWM52_001876"
     CDS             3935..4669
                     /locus_tag="IWM52_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9974123.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            4769..5635
                     /gene="rfbA"
                     /locus_tag="IWM52_001877"
     CDS             4769..5635
                     /gene="rfbA"
                     /locus_tag="IWM52_001877"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAP9974124.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            5649..6221
                     /gene="rfbC"
                     /locus_tag="IWM52_001878"
     CDS             5649..6221
                     /gene="rfbC"
                     /locus_tag="IWM52_001878"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAP9974125.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            6243..7271
                     /gene="rfbB"
                     /locus_tag="IWM52_001879"
     CDS             6243..7271
                     /gene="rfbB"
                     /locus_tag="IWM52_001879"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAP9974126.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            7369..8220
                     /gene="rfbD"
                     /locus_tag="IWM52_001880"
     CDS             7369..8220
                     /gene="rfbD"
                     /locus_tag="IWM52_001880"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAP9974127.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            8255..10288
                     /locus_tag="IWM52_001881"
     CDS             8255..10288
                     /locus_tag="IWM52_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974128.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            10332..11612
                     /locus_tag="IWM52_001882"
     CDS             10332..11612
                     /locus_tag="IWM52_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP9974129.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            11822..12628
                     /locus_tag="IWM52_001883"
     CDS             11822..12628
                     /locus_tag="IWM52_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9974130.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            12640..13860
                     /locus_tag="IWM52_001884"
     CDS             12640..13860
                     /locus_tag="IWM52_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9974131.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            13850..15436
                     /locus_tag="IWM52_001885"
     CDS             13850..15436
                     /locus_tag="IWM52_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9974132.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            15475..17613
                     /locus_tag="IWM52_001886"
     CDS             15475..17613
                     /locus_tag="IWM52_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9974133.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAESTHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(17981..18964)
                     /locus_tag="IWM52_001887"
     CDS             complement(17981..18964)
                     /locus_tag="IWM52_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAP9974134.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            19342..20733
                     /locus_tag="IWM52_001888"
     CDS             19342..20733
                     /locus_tag="IWM52_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP9974135.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            20749..21789
                     /locus_tag="IWM52_001889"
     CDS             20749..21789
                     /locus_tag="IWM52_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9974136.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            21808..22893
                     /locus_tag="IWM52_001890"
     CDS             21808..22893
                     /locus_tag="IWM52_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP9974137.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            22926..>23720
                     /locus_tag="IWM52_001891"
     CDS             22926..>23720
                     /locus_tag="IWM52_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HAP9974138.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGM
                     FAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLAN"
BASE COUNT         7694 a         3857 c         4811 g         7358 t
ORIGIN      
        1 tcgaacctat ggttaatacc ggaacttgga aaatgaaaat ggatcctaac ggctggactg
       61 cgtatacaag agacggcgga ctttcttgcc aatatgaaca tacattggct attactaaag
      121 atggtccaag aattttgaca tcacaaggag aagaaggcac ttattgatct atccagtcaa
      181 taggtacggg agaggtacat gaaagtttgg aacaaaataa aacaaaatga aaatctgatg
      241 cgctttattg agacgactca aaagcgcatc actgattcag aaatgagtac gacctctgtc
      301 gtcgttgctt attatttact cttgtcttta tttccattga ttattgcttt tggtaatatt
      361 ttaccgtttc tccacatcga tcaagagact gtactgacct atttacgaga agtgattcct
      421 gagaccgttt accagtttat cggtcctgca atcaaagacc ttttgacaca aagttcagga
      481 ggcttgttat ccgtttctgc attagcggct ttatggtctg caagtcaaag tatcaatgct
      541 ttgcaaattg cgatgaataa agcgtatggc gtagaaaata gaaaaaattt tttgatcgtc
      601 cgtttcttct cactaattgt cattgtcttg ttcatgattg ctattagtgg ggtaactatc
      661 gttcttgggt tgggacagct gattttggat gctattcagc caatcgtaca aatacccgtg
      721 aactttatcg atcagttcca aactttgaag tggccaatca cattggtagc tttatttgtc
      781 atcatgttct tgatttattt aattgtccct aatgctcagt tgaagttgaa gacgatcatt
      841 ccgggcacga tcttcgctac tgtcggctgg atgctgttat cacaagtgtt tagtatctat
      901 gctaggtatt ttgcaacgag gatcagcggt tatcagatca taggaagttt tattgtactc
      961 atgctttggc ttaattttgc tgctacgatc atcattttgg gcggaatcat caatgcagtt
     1021 gttcaagaat acgtgacggg tcatcaaatc aaaaaacgtc gagatccaag ccgaaaatgg
     1081 ttcaataaat tgaaaactaa attttctcat aaaaaaaagt agaaacaaga gttcttattg
     1141 taaatttata tcagtaattt ataataatta tgactcttaa ttaaagtagg tgcccatatg
     1201 cttgtttctt ttagtatggt tataagattt tttctgacaa ttttattatc actgatatta
     1261 acacctgtat tcaaaatcat ttcagtacaa acaggtatgg tcgacaaacc aaatgaaaga
     1321 agaataaata aagttccaat gccttcagcg ggcggactgc ctatctttat ttcatttgtt
     1381 atatcgactt tattcttatt ccgtaaaatt atccctcaat cgtatattgt accgatcttg
     1441 attgcttctt tagtaatcat agtcacagga gtgctcgatg ataaatatga gctgacacct
     1501 aaacaaaaaa gtatagggat cttgattgct tctcttatta tttattttgt agcagatata
     1561 cgtattgatt ccttcacgct tccttttatt ggatatatcc aactaggctg gcttagtttt
     1621 cccgtaacga ttttttggat cttcggtatc acgaatgcgg tcaatctgat tgacggtttg
     1681 gatgggctgg cggcagggat ttcattgatc ggtttaatta cgataggaat cattggatat
     1741 tttttcctcc atgcttctac agtgtatatt ccagttgtta ttttctgtct tgtagcaagt
     1801 attatcggtt tttttcctta caatttttat ccggcaaaga tttatttagg cgatacagga
     1861 gctctattcc tagggtttat gatggccgtc ctttcactac aagggctgaa gaacgtgact
     1921 tttgtttcat cgatttcttt gctgatcgtc atgggagtgc cggtaacaga cacgtttttt
     1981 gctatcatta ggagaaaagc aaaccgtgtt tctttttcta cagcagataa aaaacatctt
     2041 catcaccgac taatcagttt aggctttact cataaaggtg ctgtcctgac gatttatgcg
     2101 atggctttga tgttttcttt cacagctatg gtcatgaatt atactggaag aatcggaacg
     2161 atcatattga ttattgccat gttgtttgct gcaattttat tatttgaatt gattggtctg
     2221 atcaatgaga aacatcagtt tattttaaaa acattgcgat ttttagggaa taaagagtac
     2281 cgtacgcaag taaaacagaa atatttgaag aaatggaacc gtcatataga tgaataacaa
     2341 attaaaccag ccgatcctta ttagtattgt gacggcaaac agtgataaaa tttttcggac
     2401 actagataat tttatttcaa ctgtaaaaga tgaaacagca gtaaaaatcc gaatttttga
     2461 caacaattca cttcccgagt acaaaaaaag actaacagag tataggaaat atccctacat
     2521 cgatatctat tttcatgatg aaaattccgg atttggttat ggacataatc acaatctgat
     2581 agctagcgat tctcgctatg gcattatttg taacccagat attttagtgg atgaagagac
     2641 agttacagca ttggtcttgt tattgaaaga acatccggaa tgtgcgatgt tagcacctaa
     2701 gattcttaat gaagatggaa caactcagca cctcatccgt gagcagctgg ctgtttttga
     2761 ttacgttttg cgctttgtgc ctttcaaatg ggtcaaaaaa aagtttgaca aacgactggc
     2821 tgtgtttgaa tgccgaggat tgcctactga tgctaatagt tatgtacgga tgatctcagg
     2881 tagttttatg gtagctgata ttgataaatt taaagagata aaggggtttg atgagcgttt
     2941 cttcatgtac tttgaagaca acgacttgtg tttgagtttt gaaaaagcag gttacaagtt
     3001 attgtacaca cccttttatg cagttatcca tttttacggg aagggtgcgc atcgaaatgc
     3061 aaaactgttt cgcgtgtttt tgacttctat gatgaaattt ttcaataagt gggggtggcg
     3121 ttttttctga tgaaaccaga aatattagta gtcgttgtgc tttatcagca actattcagt
     3181 caatctccag cttatggacc attaagcaaa gcacttagcg aaaagcaaat tcagcttatt
     3241 atttacgata atagtcctca aaggcagcaa aatgagctgt ttgaaaaaga aaatatcgtt
     3301 tatcatcatg atccgcaaaa tcctggttta gcggtggctt ataattttgc gttgtctcaa
     3361 gcagatgaag gaacacgttg tctcgtcact ctggatcagg atacgaggct agtcgacgat
     3421 tattttgaac tattgcgtaa agtaaccttt acagatgaat gtgttgctgc agttccgatg
     3481 attttttctg ggagccgaca agtttctccc ctatattcag atcgttatat caatcgtaca
     3541 gctcaagcag tagaagttga aggcccaaca gctgaacgaa tcatggcaat caattcaggt
     3601 gttgcctggt ctgtcaaatt tctaaaaaag attggcgggt ttaatcctgc tttttcttta
     3661 gattttttgg atcattggct gttttggaaa gtggatcagt tggaaaaaac tgtggagatc
     3721 cttcctgtac gaatggaaca tgatctttcg gttttagact atgaaaaagt ttcaagtaat
     3781 cgataccgtt ctattttaca agcagaaaat cgattttatc atgaatatga tcaaaggcat
     3841 ttatccagtc accgacgaca attattgtta agaacagcta aacagttctt gaccgtcaaa
     3901 aaccggcata tctggcggat gactttgcgc tcatatgtta agaattggaa ggtgtgaatt
     3961 cgtgcgttcg gtttgtatag ctacctttaa tggaggcaga tatttaagag agcagttaga
     4021 tagtatttta tctcagcttt cagcagaaga tgaagtagtc atttctgatg atgggtcagt
     4081 agatgataca tgggatatat taactgaata cgcagaaaaa gataagcgaa tccagctttt
     4141 ttcggggcct cgtcaaggat tgatcgctaa ttttggttat gcgatccagc agacgaaagg
     4201 agatctaatc tttttggcag accaagatga tgtttggttg gagacaaaag tgaaaaagat
     4261 cactgactat tttgagcaga atccggatca gctagtagta gtttctgatt taatcattgt
     4321 ggacagcgag ctgcaagaga tccatgattc gtatttttcg tttcgaaatt ccaaagaagg
     4381 atggcttaat aatgtgataa gaagtcacta tattggtgct ggtatggctt tccggtcttc
     4441 tttaaaaccc gatattttgc cgatcccgtc agaagtgccg atgcatgata tgtggattgg
     4501 tttgctggga ggaaagaaga ctgggttcct aagagaaccg ttaactctgt accgaagaca
     4561 tgatttaaac gcgagtgaga ttgaaacaca ttctaaatta cggcaaaaaa tttcttggcg
     4621 tgttcatctg attacggcgt tgatcaaacg aaaatggttt aaacgttgat aaaagatgtt
     4681 taaatcctta atatttccat gacaaatata acaattgatg agataataac aaagtgaaat
     4741 ttgaaatcaa aaaggagagc agatatccat gaagggaatc attcttgctg ggggcagcgg
     4801 aacacgtctg taccctttaa caaaggcaac atcgaaacaa ttgatgccga tttatgacaa
     4861 accaatgatt tattatccaa tgtctactct gatgttggcg ggaatcaaag aaattctgat
     4921 tatttcaacg ccacaagata caccacgttt caaggaatta tttggaaacg gaaatgattt
     4981 aggactgcag atcgaatatg cagtacaaga aagtccagat ggattggcac aagcgtttat
     5041 cattggagaa gaatttattg gagatgatag tgtctgcttg gtattagggg acaatattta
     5101 ctatggtggc ggtctgtcta aaatgttgca acgagcagct tcaaaagata caggcgcgac
     5161 agtatttggc tatcatgtca atgatccaga acgctttggt gtagtagaat ttgatgagaa
     5221 tatgcaagct ttgtcgattg aagaaaagcc tgctcagcca aaatcaaatt acgctgtaac
     5281 tggcctttat ttttatgaca atgaggtaat ttctatcgct aaaggtatca aaccttcaga
     5341 acgtggagaa ctagagatta cggatgtcaa taaagcatat ttggataaag gaaaactatc
     5401 cgtagaactt atgggacgcg gatttgcttg gcttgataca ggaacacatg aatcattgct
     5461 tgaagcatct acctttatcg aaacaattga aaaacgtcag aacttaaaag tggcttgttt
     5521 ggaagaaatt gcttaccgta tgggctacat cgataaggaa caattgctgg cccttgcaca
     5581 gccattgaaa aaaaatcaat acggtcaata tttattgaat ttggccgctg aataaggagt
     5641 ccttacagat gaaagtaaca gatacaaaat taacagatgt caaaatcgta gaaatggacg
     5701 tctttggcga tcatcgcggt ttcttcacag aaagttattc aaaagcaaaa tttgcagaac
     5761 atggattgaa atatgacttt gttcaagaca accattcact atcagctgaa gctggtgtcc
     5821 ttcgggggct tcatttccaa aaaggagaag ctgcacagac aaaattgatt cgggtagtca
     5881 ctggagcagt tttagatgta gtagtggata tccgcaaagg tagcccgact tatggtcaat
     5941 gggacggatt tatcttatct gaacataatc atcgacagtt gcttgtacct aaaggatatg
     6001 cccatggatt cgttactttg acaccaaatg tgaacttcct ttataagtgt gataattact
     6061 ataatgcaga agcagatggg ggcatcgcat ttgatgatcc agatttagcg attgattggc
     6121 caattgatcc atcaagagcg attatgtcgg aaaaagatca ccgtcatccg acattaaaag
     6181 aatttgaagc agaaaatcca tttgtttacg gagaaatcta agaattaagg agaaattttc
     6241 ttatgaaaaa catcattgtt accggaggtg ccggattcat cggctctaat ttcgttcatt
     6301 acgtggtaaa caatcatcca gaagtgcatg tcactgtttt ggataaactg acttatgcag
     6361 gaaacaaaga gaacttggcc ggacttccta gcgatcgcgt agaattggtc gtaggagatg
     6421 ttgcagatgc agaattagtg gaccgtctag taaaagaagc ggacgcagtt gttcattacg
     6481 cggcagaatc ccataacgat aattcattaa aagatccttt cccatttgta caaacgaata
     6541 tcattggtac gtatacactt ttagaagctt gccgcaagta tgatgtacgc tatcatcacg
     6601 tatctactga cgaggtttac ggcgatcttc cattacgtga agacctgcca ggacatgggg
     6661 aaggcgaagg agaaaaattc actgcggaaa caccatataa tccttcaagt ccatattctt
     6721 ctacaaaagc tggttcagat ctgttagtaa aagcatgggt gcgttctttt ggcttacgtg
     6781 caaccatttc aaactgttca aacaactatg gtccttatca gcatattgaa aaatttattc
     6841 ctcgccaagt aacgagtatc ttaagcggta ttcgtccaaa attatatggc gaaggaaaga
     6901 atgttcgtga ctggatccat acgaatgatc attcttcagc agtgtggctg attttgacga
     6961 aaggcagaat tggcgaaacc tatctgatcg gtgcagacgg tgaagaagat aataagaccg
     7021 tgatggaatt gatcttagaa atgatgggcc aaccaaaaga cgcatacgac catgtaaacg
     7081 accgcgcagg gcacgatttg cgttatgcaa tcgattcgac aaaattgcga gaagagctag
     7141 gttggcagcc tgagtttacg aacttccgtg atgggttggc tgacacgatc aaatggtacg
     7201 aagaacatga agactggtgg aaaaaagaaa aagaggccgt tgaagcagca tatgccaaaa
     7261 atggacaata atacagagga aatttcactg tattaatgga aaaacacatg tgaggcaggc
     7321 gctcgcctgt ctctttttta aaataaaaat caaggagcgg ataaccatat gtttttatta
     7381 actggaggaa atggacaatt aggtacagaa ttgcgccatt tattagatga aaaaaatctg
     7441 ccatatgttt caacagatgc aaaagacttg gatatcacag atgctgagaa aacaatggct
     7501 tatattacag aattaaaacc cgatgttatt ttccattgcg cagcttacac agcagtagat
     7561 aaagcagaag aagaagcaaa agaattagat gagaaaatca atgtcgatgg cacaagaaat
     7621 gtagcacttg cagcaaaagc tgtgaatgca caattcgtct atatcagtac agactatgtt
     7681 ttcgatggaa aaaacaaaaa tgtagaatac gaagtagacg accaaacgaa tccactaaat
     7741 gaatatggtc gtacgaaatt attaggcgaa aaagcagtag aagagatttt agaagactat
     7801 tatatcatac gaacttcttg ggttttcggg atctatggac acaatttcgt cttcacgatg
     7861 caacgcctag cggaaacccg cgacaaactg actgtagtaa atgaccagtt tgggcgacca
     7921 acttggacac gtactttggc agaatttatg gtatatgtca tcgaagaaaa agcaccattt
     7981 ggtatttatc atttgtcgaa cgaaaatagc tgttcttggt atgagttcgc aaaagaaata
     8041 ttgaaagata cagatgtaga agttgcacct gttacgtcag aagaatttcc gcaaaaagca
     8101 acacgtccgc agtattcagt catgagcctg aaaaagacag aagcgttagg ttttgtcata
     8161 ccaacttggc aagaagcact tgcgcaaatg ctggaagctg ccaaaaaaat gaataagtaa
     8221 tttccttttt atcaaataga atttggagca tgatatggaa aaacaaacaa gaagtgcacg
     8281 acgaaaagaa aagccaaaag cgaatcaggc agattcgaat ttggtgcttg acctgtttct
     8341 aaaactccgc tatcttattg gcgttttagt catcgtggtc atcgttactt ttaatttaaa
     8401 cggaagttca cttggtgttt gggataaata tgtgtcgcaa agagacgatg ggaaaaaaac
     8461 tgatgtgatt tttggacaga atcgggaagt gcggtcagac gaatggctgg tgcagacacc
     8521 tttttacctc tcacaagctg aagaggactt ttcgttggtg aacgaggatt attcattaaa
     8581 cggacaaaac atgatcattg cgtataattc tcctgtaaag gatattacgg ttatcggaaa
     8641 accattcaac tggggattct ttttcctcgg aagagatcga ggactttcct tttattgggc
     8701 gatgaaatta gtcgtcatgg tcttgcttgg attcgaagta ctgatgatat taacgaagag
     8761 gaaaaaagct ctatcgctaa tcggtgcatt tgcgctgaca ttttctccag cagtccagtg
     8821 gtggttcatg caacatgtgg gcgatttgat tttctttact ttgggactga tgattgcttt
     8881 ttatcattat ttctacaatc atgataaaaa atggctacga acgttgatga tgcttttggt
     8941 ggttattttc ggtattgggt ttattttagt gatttaccca gcccatcaag tgatgctggc
     9001 gtacttgctg attttttatt ttgttggcct gcttatttac tatagcaaga aagtttcatg
     9061 ggatctgttg gatgtagtgt tgattggagg ggcagtttta tttattggcg gagtcatggt
     9121 tcatttctgg ctaacgtcaa aagacgcatt gttagcttct ttgaatacct tatatccagg
     9181 aaaacgggta agtactggag gaaagtggac gattggcgaa tttttttctt tccttacaaa
     9241 ttggaaaatt ccttttgaag atatcaacta ttcgaataac tctgaagtgg ccctgttcta
     9301 tcactttttt ccaactgtat tcctagcaag tccatttgtg ttatttggca aaaaagatag
     9361 cgaacaaaag ttactgggac gtattttgat gattttttgt ctgttttcta tcttttggat
     9421 taccgttggg ttgccaaaat ggctagcaga aatcactctg ctgtcttatg ttcctccatt
     9481 aagagccatc cttactttta gttttgcggc ttgtttatta tcgatctggt ttatcgctta
     9541 tatttggaga gaagatgtta ttcctatttg gtataaattc attttgttgg cagtaaatgc
     9601 atggtcttat ttctatgcga ttacgcattc gaaaatggat aattattttt cggattttga
     9661 aaccatcgca gttgctgtgt tgggaacagt gattttagct tttgcattat ttggctggag
     9721 acgcttgttt tacctcgctt ttgtcgcctt agttatcgta tctggctttt tcgtcaatcc
     9781 agtcgtagaa ggtacaggag cgatttttga aaaaacactg gcaaaagaaa tccaaaaaat
     9841 cgatcaacaa gatcctggta aagtttggtt gtctgaagat gaattatata acttcacccc
     9901 aacattggga gtgaaatcct tcaatacagt acgcttttat ccagatatag ctgcatggaa
     9961 gaaaatcgat ccgaaagaag aatacgagaa gtattataat cggtatgctc atacaagagc
    10021 ttttatcgct aaggaagata caaagttcat tttagatcgt ccagatatgt tttcggtcac
    10081 gttgaatttt gaagacgcag aaaaattagg gatcgactat gtgatcagta aacgaccatt
    10141 aactgattac aaccaattat atgaagcgca gttcacgcag ctatatggac cggataaaga
    10201 cggttatatg atttttaaag tagaatatcc ggattatcca gatgtaccac aaacacaaac
    10261 tcaggaaaat cagctagtcg tacagtaata ggctagctga gaataatcct gaaatattag
    10321 tagaaaggag agtgaagata attcaagcaa tcgtacaaaa atttgtcaac aaaatgagtg
    10381 tcggtttctt gcttttgttg ctgacgctag tcttttttgt ttggacagca ggggtaggtt
    10441 ttccagcaac tttgtttata tataacaata gttcattact tatcgctttt agtgtggcta
    10501 ttttgctcat tttaaatgtt tataaagcaa aaggcgtcga ttttctttat ctaggtgttg
    10561 cgcttgccct tttcattttc ttttcttact ttcgggaatt aagaaatgag tctttatttg
    10621 gagacttact gtttcctttg attattttat tttgtttagt aattaaatgg atcgagtgga
    10681 caaaatggga tcgcggtatt tatctgattg tctttacggt cttcctcgga atcacgttat
    10741 atcgtgtatt tacagagatc caagtaccag aaggacaaag catctgggct ccaggcaatt
    10801 cgctttctga tatttggatc aatacgaata caatcggaag ttctttgatg actcttgctt
    10861 tattgatcag tggatttgcc agttcttttg aacgatggta tgttcgaatc atcggtggga
    10921 tagcagtcgt tgcagccttt ttaggtattt gggtctgcca atctagaggt gcattagtcg
    10981 cgttgattgt ttttgcgata ttggatcttt tacctaaatc gttgatgcgt gtcattcgtg
    11041 cgccatttat tgcctacacg gttacgattc ttttagcttt gccgatttct tatctggcgg
    11101 ctgtctctga aaaagtcaat ttgtttaccg atcgagaaga tatttggcat aaattctatc
    11161 agacgctagg agaaaaatct gaacaagttt tactaggaat gaaaacattt atcttccagc
    11221 gaggaaacca atttttagga aaccacaata gttataattc tattttgaat atctatggat
    11281 tgataggatt tgggattgct gctttgctgc ttatcctttt tattggtcgc ttaacgttga
    11341 aagcagattt atccaacggg cagatgactt ttatctgggc cttttttgcg gtgatgatgc
    11401 agtcatttat ggaggataca ttgacttcat acccatgggt tccgattatt tatatcctgt
    11461 tggctatggc cgttcatcga tatgatgaac cagaagttgc ggaggaattc tatgttatgg
    11521 aagaggcata tgacgatatg acttatgatg atacatctta tgaggaacaa ccagatagcg
    11581 ctcattcttc caggatccaa cgctatcatt gataggcaca aaatacgaga gaggctgtga
    11641 caaccgtcgc agcctttttt ggtttattgc aattgtctag catcttctgt aaaatagaaa
    11701 cgttagagaa aatcttgtat catagaaaat taagaaagaa ataagaacgc agctcggtac
    11761 caaaaatatg gtaaaatggc acttgactgt aattcactga aaatcgcttg gaggaacaca
    11821 aatgtttaaa gatttgtatt tatttatgaa ggacatttat cagaacagaa aattattgct
    11881 acagttttcg atcaatgact tcaaatcaag atatgcagga tcgtttttag gaatcttatg
    11941 ggcgtttatc aatccgctat ttactgtatt gatttattgg ctggtatttg gtgttggact
    12001 gaaagcacaa atgatcgatg caaaataccc atttatcgta tatttagtta ccgggatgat
    12061 tccctggttc ttcttctcag atgcatttgc atcaactacg gttgtttttc gggaatatac
    12121 ttatttagta aagaaagtcg tattcaatat tcgtatcttg cccacgacga aaatcctatc
    12181 caatctgtac acccatttat tcttcattct gattggcata gctgtcgcgc ttataaatgg
    12241 tatatatcct tcagcaatgt ctttacagct gatttattat ttattttgca tgtgtgcttt
    12301 cttgacagga ttgacttggc tgacggcatc gatccaacca tttttacctg atatcatgca
    12361 attaattacg atcgtgctgc agttgatcat gtggacatta ccgatattat ggagccctaa
    12421 tcagtttgat tcaggtcttg taagcatatt gaaattaaat ccgctgtact atgttgttca
    12481 aggttatcgt gagtcatttt taagtgaagc ttggttctgg gagcatggaa catatacttt
    12541 gtatttctgg gttttcacgt tagtaatgtt cgtaatcggc tctgcagtgt tccgtagatt
    12601 gaaaccacat ttttcagatg tgctgtaagg agaatagcta tgacagaata tgcaattaga
    12661 ctaaaagata tcacaaaatc ttataatatg tacgcaaaac cttcggatcg atttcgtgaa
    12721 gccctcaatc cttttaagaa atcctatcat gatgtatttt atgcgttgaa aaacgtgaat
    12781 gtagaaataa aaaaaggtga gatgatcggt ttcgtgggag aaaacggatc gggaaaatct
    12841 acaatgttga aaatcattac gggagtactg acaccaacat ccggcacgat ggaaatcgaa
    12901 ggaaaaatct cagctttgct agaactagga tcaggattca atcctgagta ttctggctat
    12961 gaaaatatct atttaaatgg aatggttcta ggattttcaa gagaagaagt cgatgaaatg
    13021 gtcgatgata tcattgaatt tgctgacatc ggagaccata tttatcaacc tgtaaaaact
    13081 tattcaagcg ggatgttcgt tcgtctagct tttgctgtag caatcaatgt gaatcccgat
    13141 attttgattg tggatgaagc attggcagta ggcgatcttg aatttcaact aaaatgtatg
    13201 gaaaaattta cagaaatcaa gaactcggga aagacgattt tattcgtttc tcatgatatc
    13261 agttcgattc gtcgtttttg tgatcgttcc tattggttga aaaacggaga agtcgtcgaa
    13321 tatggcgata cgatggaagt aacgacgaat tatgaaaact ttttaaagaa aaaatcagtt
    13381 aaaacagtgg atcggaataa aaaccaagta gaacggttcg ccgctccaga tatcgtcgac
    13441 gtgacgaaag cggaattgtt ggataaagac aaaaatccgg tggatatgct ggaacaagga
    13501 gaaaaactat acgttaaagt aacttatgaa gtaaaagatg atacaatcaa aaatccggtt
    13561 ttaggaattg cgctacgaac agtcgacaat ttttatgtat gcggacttaa tactctacta
    13621 gatggtatca agattccttg gaaaaaagga gagaatatct tttatctgga gtatgaaaag
    13681 ttaggtttgt tagctggtga atattatttt gacgttgcgg tctttgaaga aaatgcaacg
    13741 gttcctttgg tatataagac aaagtacatg aacttgtttg tcagcggttc ttatatcgga
    13801 gaaggcatcg tggtcttaga ccataaatgg gaagaaggga ctcatagcaa tgaaatatga
    13861 ttttgaaatg gacctagatg aacaaagcag tgtgggaaaa attgccgcac agatcaaacc
    13921 aggaagtaaa gttttagagt ttggtcccgg aaacggccga ttaacgaaac atttaatcgg
    13981 agcaaaacaa tgtgaagtga gcattgttga gttagataag gaattgttcg attttgtcag
    14041 cgaatttgct caagatggat tttatggtga tatcgaaagt tttgagtggg caaactatta
    14101 tgctggacag acatttgatt acgtcttgtt tgcggatgtt ttagaacatt tggtagatcc
    14161 tggaaaaaca ttgaagaaag tcagagaatt tttgaatgaa gaaggagaga tcctgattac
    14221 attccctaac ttggcgcata attctgtcat gatcgatttg ttcaataacc aattgccgtg
    14281 ggcttcttat ggtctcctag atgaaaccca taattctttt tatactcatg atggcttcca
    14341 aaaagttttt gaaaaagcgg gattattcat taatattgaa gattatttgt acttagctgt
    14401 aggtgataca gaattaaaat caacttatga agaattgccg gaagctgtac gctatgattt
    14461 caaaatgcga ccatttggtg aagtctacca atactttttc tcattgatga aacatccagt
    14521 agcacaaagt tccattgcag agccacaaaa ttctaattat gtaaaagtgt tagaagtaac
    14581 ccagcaaacg aaacaagatg aaacaatcca acaaatacca ttcaataatt tcacagggga
    14641 aaatgagaca ctaagtttcc ccgtatcaga aacaacagaa agaatggtgt ttcgcttcgc
    14701 gcaacaacct agctttatcg aattcagcgc agaagctgcc ggagaaaaac taacatttat
    14761 cgattcgaat gctgtagtca aaacagtaaa tgattgttat ttgtttgatg ggaaagaatt
    14821 accagagttc gttctaacgg atatttctgg gaaagaagtg actatccatt gtcactatcg
    14881 ctttatcggg gaattgacac caacaatgaa agaattgttg gaaacgattc gtcccatggc
    14941 tgaagtcaca aagcagcttt ctgagaaaaa tgacgaatta gaaagagaaa atcatcatct
    15001 tcttgaggaa aatacaagac tagaccatac gttaaaaaca accacaaatc gctattgcac
    15061 attgttggaa agtgatgaat ggacaataaa acatagacta ggacgacgta agaaagaaac
    15121 ttcgaaaaaa atccaagaaa aagaactttc tatatgtatt gacgagaaaa tctgggatgc
    15181 agaaacaaaa atattaaaaa tcatcggatg gggaatcgct aattcggatc gccagccctt
    15241 gtcctataag ttatcagctg accaatcgcc atttttcgaa gcaattcctg tttcacgaga
    15301 agaagtcaat caagcagaac agctcgcaaa aggaacagaa gcgggatttg agttacgaat
    15361 cctttgcgag caagaacgtt cattcttagt ggaagcagta gctcaaaatg gccaatcatg
    15421 gatcattgaa atgtaaataa tgattaactt ataaagataa ggagtaaacg aatcgtgcaa
    15481 aaaaaagaaa ttgccgtatt tatcgatcaa atcactcgtg aacgggccac tggcgatttg
    15541 cttattgtgg gctgggcaat tgatgaagtg accaaagaaa tccctacgat aaaggtagaa
    15601 aaagaaaatg tgattgctga atcaacacat gtcgtaagat tggatatcaa tcatttatat
    15661 aatcttgatg ttaagaccca aagcggtttc aaggtacgtt tatcagggaa aatgagagga
    15721 aaagccatct tagacttcca aacagcgaga catcaaaatg gcatagctgt gaaattaaac
    15781 ggtaaatacc cttatgatga cggcattgaa tcttcatggg aaagaaaaaa aagattactg
    15841 aaaaaaggga taaattatgc aaggactcat ggcgtaaaaa aagcgatccg tcgggtcaaa
    15901 ttggaattga agccaggatc gatcgactat gcagagtgga tcagtcgtca tgagaaacca
    15961 gatttgaaag agcaaaggaa agaagcacaa ggatttgcgt accgtccatt gatttccata
    16021 cttatgcctg tctataacgt tgaaatcaaa tggctcgaaa aatgcattga ttcggtactg
    16081 gatcaaacgt atgatcattg ggaactatgt atcagtgatg atgcttctac tgatccagct
    16141 atccgaaagt gtctggaatc ctatcaagca aaagatgata gaatcaaagt cgttttccga
    16201 aaagaaaatg gacacatcag tctggcaact aattctgcat tggaaatagc agaaggagaa
    16261 tttatagcac tcttagataa tgatgatgaa ttaccgccat ttgcattata tgaagtggca
    16321 aaagtgctaa acgtacatcc agaactggat gtgatctaca gtgacgaaga taaaatcgat
    16381 gcagatggca atcgctttga tccgcatttc aaagcagact ggtcgcctga tacattgatg
    16441 ggaaataatt atatctctca cttaggcgta tatcgaacaa gcattgttaa agaacttggc
    16501 ggtttccgta aaggctatga aggttctcaa gattatgatt tggtccttcg tgtgacagaa
    16561 caaatccctg cagatcatat ttaccacatt gaccgagtct tgtatcattg gcggacgatc
    16621 ccaggatcaa ctgcaagtaa tggcgaagcc aaaagttata tctatgattc cggagtgaaa
    16681 gcattgacag atgccctgtc tagaagaaac atcaaaggaa gcgtacgtcc tggcagaatt
    16741 tctggatttt atgagattgc ttatgacgtc ttgcaagagg atttggtcag tgtgatcatt
    16801 ccaacgaaga atggctatga agatctaaaa acttgtgtgg attcgatcat cgaaaaaacg
    16861 tcttattcaa attatgaaat cattattgcg gataacggaa gtacagatcc aaaaatgcaa
    16921 gaattatttg cagaatacaa gcatcagctg aaagaccgct ttatcgtaga attgattgac
    16981 attccattca attattctcg aattaacaat ttagcagcag aaaaagcaaa cggcaaatac
    17041 tttttattct tgaataacga tacagaagtg attgagccag attggatgac ggtgatggtt
    17101 tcttatgcac agtttgatcg aatcggctgt gtaggagcaa aactgtttta tccagatgat
    17161 acaacgcagc atgcaggagt attattagga atcggtggtg ttgcagggca tgcattgaat
    17221 aactatgatc gaacacattg tggttatttc ggccgattgg tcattgacgt caattactta
    17281 gcagtcactg cagcttgtat gatggtcaag gcagctgact tcaatgctgt taacgggttt
    17341 gatgaaacat tagaagtagc attcaacgat gttgacttgt gcttgaaagt ttatgaactg
    17401 ggtcgttaca atgtctatgc acatcaagca gaattgtacc atttcgaatc taaatcaaga
    17461 ggttatgaag atactccaga aaaacaaaga cgttttgctg gtgaaatcaa aaaaatgcaa
    17521 gataaatggc cagcatatat tgcgcatgat ccattttaca atgataattt aacaaaagac
    17581 ggaatcggcg acttttcgtt gagaccagat taaactataa taaaaagctg ttatgttgtg
    17641 attcgctcgc acataacggc tttttatagt tttttgtcaa aaaaaggcag aaggtgccga
    17701 ctcaaaaaaa taagtcgagg ttgggacaga agtgtttaac tccgagaaat aagaagaaat
    17761 tcacgaaaat tgcttttcaa atttttgtga atttcggctt atttaagaag aagttgcttc
    17821 tgctcccacc gtttattcgt ttttaaagaa tgagacaaaa gtgtttctta cttttgtccc
    17881 acgctcttat tcatgctcag agcgccaata gatacaaaat catagccaaa gtttcacgaa
    17941 ccctagctgt caaagaatcg atattttatt ttaatatagg ctattttaaa ataaatggtt
    18001 taaggtattc ctctacaaaa gccactctat caattatcat gggtttaaaa taagaagtga
    18061 tggagataac cagattgttc ttagcgtcaa tataaagaac atttccacta ttaccaattg
    18121 ctgaatatat ttcgttctca gaatctaata cccaccataa atagccatat ctcatatttt
    18181 gtaagtcttc taaagcactg gttctaggtt tgaacatctc gtttatccac atgggtggta
    18241 cgatttgttt tcctttatac atccctttat ttaggcataa tacaccaatt tttgccagat
    18301 cacttgccga taaacacaaa ccatatcctg ccgtcccgat atttagtgga tcacaaaacc
    18361 agacattggt tttaggagac ttatccattg taaactgctt atgttcttca gctgtttcag
    18421 cataataatt gacatggttt ggtatcccta taggatcaaa tagatactta ttggcaaaat
    18481 caacagttgt tagcttactt gtctgataca acaagccagt taatatgtgt aaacagacag
    18541 tttgatagtt gaattcgccg gttatacctt ttcttcctcc caataaatct agtgatgttt
    18601 ttgtccaatc ttcacttgaa cagacttttg tccatggatc gcctttacat ttgtagggtg
    18661 ctttcattgt tagcaagtgc ttgattgtaa tagcgtaaat agttttctcc ccgcgtttca
    18721 ctttatactc tggaaaataa tctaatactt tatcatcgat acttttgatt aatccttgct
    18781 ctaaagcaat gccaatcagc aaagatacaa tacttttcgt tgctgacatg atatgacaag
    18841 tatcgtcttt tttataacca ttccattcat ctgtataaac ttctacccca tccttatatg
    18901 cgacaatttg acatatattt ggttgtcgaa aaccaataaa atcatgcaac tcttgtttat
    18961 tcataagata ttacctcact ttcttctata agcataatgt cattaaaaat cgatttcaat
    19021 tttttcgatt cttcacgtaa tacaggtatt cttatggact agacagcgct ttgggaagat
    19081 ggattctctg aaaaatataa aggttcactt tatgagtgga taaaaaaagc agcaaatact
    19141 tcgacttctt taatttacca acattagtga aaatcgattc atctggagat ttgtaattta
    19201 gagtccttgt cttgattaga ggtgtataaa gctttttatt agacattttt aaattccatt
    19261 acaatttgct tataaactag gtttttaaag gtgactatgg taagataata gagatattat
    19321 tttgtgatta ggagtgtata tatgaataaa aatggagaat ggagtgctgc acgacggata
    19381 ttcattatat taatggatgt tattgtatac aatgcagcaa tatatagtag tttcttactg
    19441 aagtttcgtg gagagatccc attacgaaat ttagaaactt tccaacactc agcaattatt
    19501 atttcagtga tttttattgc gttgaacatt ttgttgggtg catatgtttt ttataatcga
    19561 atgatcagtg acatcgtgtt tgtcacagtc atcggtcagg tattgatgac actagggatc
    19621 atggtagtga cttttgcggg ccgctggttt gcttttccac gtcttgttat cttactttca
    19681 tttgttctcg gtacgctttt cttaagtatc tatcgtattc tcgtatataa actttattta
    19741 cgcgtaagtc gagataaaaa ggtcgtcatt attgggttag aaaaggatgt cgcaccagca
    19801 atccaaaatt ttggttcaaa gaaaaacaac aaacataaag tcgaagcagt cattcttgat
    19861 aactattatg caaacatcaa aaatatgatt aaagacatcg atattgtcta tcttgcttct
    19921 catatcgaag aaactgaaaa aatcaaaatc tatcaattag ttactaaaaa tgaaaaaaaa
    19981 ttgtttttga atacaacatt tgaaaacttg acattgatca atccaaatat tatgaatttc
    20041 gaagatgaaa gtattattga agcatctgga tttagaatcc aacctgaata tgaactattc
    20101 aaacgtttgt ttgatatcat cgtttcactt gttttattag ttattgcttc accatttatg
    20161 ttattcacag caattttagt caaagtgaca tcaccgggac caatcatcta taaacaagtg
    20221 cggatcacaa aaaatcaaaa ggaattttca atctataaat tccgctcgat gaccgccaca
    20281 gccgaagcaa aatctggacc agttttagct aagagcaatg atgcacgagt aacgcctgta
    20341 ggtaaattta ttcgagctgt ccgctttgat gaattaccac aaatcttcaa tgtgctgaaa
    20401 ggcgatatgt cgatcgtagg accacgtcct gaacgtcctt tcttcgtcga tcagttcaat
    20461 gcagaaaacc cttattacta cttacggcat aatgttcgtg ctggtatcac tgggtatgca
    20521 caagtctatg gaaaatatgt ctctgattac aacagtaaat tacgttttga tttactttat
    20581 atcaaaaaat attcattgat gcttgattta aagatcttgc tgcaaaccat caagatccta
    20641 tttgataaaa tgtcctctca aggacttgaa gaagacgaaa atgtgatctc attggcagaa
    20701 attgcttttc cgaatgaaaa aatctttcgc tagttgggag gcgtaaaaat gaacaatccg
    20761 aaattcagca taatcgtacc agtatacaat gcagaagcaa ctattggtcg aacgattgat
    20821 attctaaaag aattggatta tgaagagtac gaagtcgttt tagtaaatga tggatcaaca
    20881 gatcaaacac aggaaatcat tcaagaactg attgaaaatg atgaacgttt tgaattgatt
    20941 actacgacca atcaagggcc cggattagca agaaacgaag gaataagaca gtcaaaagga
    21001 gaatatctct tattcttcga tgcagatgac acgccagtaa aaacgatctt gtcagattat
    21061 gatcatttgc tagaaaagag tccaaaagcg gatcttgttg ttagttcgtt tgtttttcgt
    21121 acaatggacg agggaagaat cgtttccgaa aaagaaaact tagttacaga atgtcactat
    21181 ctaactcacg aagagtttat aaaagacatg tatcagttaa tgaatgaaca gttgatgtat
    21241 gttgtatgga acaaatgcta tcgtagcgat attctgaaaa ataaccaaat tctatttaaa
    21301 acttataata gttgtgagga tcgaatattt aacttacatt attatgaata ctgtcatcaa
    21361 gtgatcatga acccaaaagt agaatatatt tatgaatttg aaggtgggaa ggggatcact
    21421 aatcaatacc gacctaacaa gttcgaaaca tttaaagaat tctattcatt agcaaatact
    21481 gtaacagacg atattaataa agacggtatg gcggcgcttc ttttaaaagg gacaacttca
    21541 gttatttttt ctatttatag tacctctgca agaacttcaa aagagaagaa agacgaagca
    21601 aagcagatat tgagtgatcc agtgatccga gaagccaagc aaattgctaa gacggattct
    21661 tcagcaaaaa aaataacaaa gttgctttac aatatgccag ctccactatt tttatctgct
    21721 gtaaaagttg gaagcttcgt tgaagtgaaa atgccagggt taatggctgc tttgaagcga
    21781 gtatattagt taatggagga aaatataatg aaaagaattt tgcattttca gggacgaatg
    21841 ggtttaggtg gagcagagtc tttcatgatg aatttatatc gtaaaattga tcgtacaaac
    21901 tatcaatttg attttttgat ttatgaagat tatgcagatg ttcaagacta tcattcagag
    21961 attgaaagat taggaggccg tatttttgtt gtacctaatc ctaagaaaaa tatttttaaa
    22021 tatttaatag aagtgaataa gttactaaaa aaagaatctt tttctatagt tcacaatcag
    22081 gtttattttg gcgggggaat taatttatgg ttagccaaaa agaatggaat cagacagaga
    22141 attgctcata gccatgcaac tgaagatgga aaatctcaaa atattgtaat gaatgtattg
    22201 cgtaaatttt taaccaaatt gttgttacaa aatgcaacag actaccttgc cgttagtcaa
    22261 gaagctggtg aaagtctgtt tcaaaaccat ccctttgaga tagttcataa tggtattgat
    22321 ttagagcttt atagtaaaaa ttcagaagct aaggttaata agagaaaaga attggatatc
    22381 tcaatgagta cttttgttgt gggaaacatt ggacgattag agaaacaaaa aaatcaatct
    22441 tatttgttag agattttttt agaaatacta aaaatgaatt ctgattctta cttgatttta
    22501 gtaggtgaag ggtcacttag aaataaactg aaagccaaag cagtagaatt ggatatagag
    22561 aaaagagtaa aatttttagg agaaagaaac gatattcccg agttattatc tgtgatggat
    22621 gtttttgtaa tgccttctct ttatgaaggt ctgccaatgg ttggattaga agcacaagca
    22681 gcaaaattaa aattagtctt agcagatact atttccaaag atacaaagtt aacaaataat
    22741 gtggattttg tatcattaga tgaaacacca caagaatggg caaaaaaagt aataaaagag
    22801 ccgtatgcta ataaaataac acaagaacta aagagttatg atgtttcgta cactactgaa
    22861 caaatggaga gtatatatag tagagatcat taaaaatgtt caagatattt taggagtgat
    22921 tatttttgaa aatatgttat ttgatattgg catacaatga tattggcaac ttaaagagat
    22981 tggtcaatcg attgaacgaa aatgcagttt ttattattca tatagataaa aaaactgata
    23041 tagaaccatt tattgaagca tttaaatcat atgataatgt tgaattttta tgtaataaaa
    23101 accgtgttaa aatttattgg ggtggttttt ccattgtaca agcagaaatc aatttagtaa
    23161 agagagcatt acaaaatgag aaatatttaa aatatgtatt attatctggt gctgattatc
    23221 caatcaaaga taatgagtat atttataatt attttaaaaa gaatagttca gtggagttta
    23281 ttcgtggaat agatctagat caaattaaac ataaagaatt ttattataaa catatagatg
    23341 tatatcaaaa acatgattat cctagaatta ataggaataa cacaactgca tttaaaattt
    23401 ttagagctat aattaatcgt tgtctgagga tgattaaact tcccccaaaa attagacacc
    23461 ataaatttga tttgtatcat ggttctcaat ggtgggcttt gtcgaaagaa tgcctaacag
    23521 aattaataca aatgtatgaa caaaatcaac aagactacct gaattttaaa ataggtatgt
    23581 ttgccccaga tgaaaaattt tttcatacac ttttttttaa ttcctcgttc aaaaataaaa
    23641 atgttattgg aggaccagat ttaccacttg aattgaaaaa tattgaagaa actactttac
    23701 aaacttcgaa attagcaaat
//
LOCUS       DACDYI010000052        23489 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.052, whole genome shotgun sequence.
ACCESSION   DACDYI010000052 DACDYI010000000
VERSION     DACDYI010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.052"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="IWM52_001892"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="IWM52_001892"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAP9974139.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="IWM52_001893"
     CDS             complement(1172..1867)
                     /locus_tag="IWM52_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAP9974140.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="IWM52_001894"
     CDS             complement(1880..2596)
                     /locus_tag="IWM52_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAP9974141.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKASKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="IWM52_001895"
     CDS             2926..3534
                     /locus_tag="IWM52_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAP9974142.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="IWM52_001896"
     CDS             3859..4182
                     /locus_tag="IWM52_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP9974143.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="IWM52_001897"
     CDS             4364..5734
                     /locus_tag="IWM52_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAP9974144.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="IWM52_001898"
     CDS             complement(5864..7165)
                     /locus_tag="IWM52_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP9974145.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9510)
                     /locus_tag="IWM52_001899"
     CDS             complement(7519..9510)
                     /locus_tag="IWM52_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAP9974146.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="IWM52_001900"
     CDS             complement(9654..9920)
                     /locus_tag="IWM52_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9974147.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="IWM52_001901"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="IWM52_001901"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAP9974148.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="IWM52_001902"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="IWM52_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAP9974149.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /locus_tag="IWM52_001903"
     CDS             13264..13827
                     /locus_tag="IWM52_001903"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP9974150.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="IWM52_001904"
     CDS             complement(14080..14601)
                     /locus_tag="IWM52_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAP9974151.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="IWM52_001905"
     CDS             complement(14604..15446)
                     /locus_tag="IWM52_001905"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAP9974152.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="IWM52_001906"
     CDS             complement(15439..16164)
                     /locus_tag="IWM52_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAP9974153.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="IWM52_001907"
     CDS             16340..16636
                     /locus_tag="IWM52_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974154.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17204..18796
                     /locus_tag="IWM52_001908"
     CDS             17204..18796
                     /locus_tag="IWM52_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9974155.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="IWM52_001909"
     CDS             18809..19549
                     /locus_tag="IWM52_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9974156.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19717..21066
                     /locus_tag="IWM52_001910"
     CDS             19717..21066
                     /locus_tag="IWM52_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9974157.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21194..21784)
                     /locus_tag="IWM52_001911"
     CDS             complement(21194..21784)
                     /locus_tag="IWM52_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP9974158.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21931..23283
                     /gene="brnQ"
                     /locus_tag="IWM52_001912"
     CDS             21931..23283
                     /gene="brnQ"
                     /locus_tag="IWM52_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP9974159.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6715 a         4739 c         4479 g         7556 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaagcctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagccattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaaataaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttct gaaaattttg gaatcttagt tgtgtttttc agaaaactct cttaatatgg
    19681 aagtaattaa acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag
    19741 cgtggactga aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg
    19801 ttgtttttag gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat
    19861 cttattactg gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca
    19921 aacaccaata accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct
    19981 ttcatcactg gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca
    20041 gccatcggaa tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg
    20101 attgcattag gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta
    20161 gagttttggt ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct
    20221 tatatgatcc tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg
    20281 tctcatggcg gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta
    20341 acctttgctt ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct
    20401 gaaaaagtgt tgccggaagc tatcaataat attcctatca ggatcatctt attttactta
    20461 ggatctcttt ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt
    20521 ccatttgtag aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta
    20581 gtcgtattaa gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg
    20641 cttcgttctt tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat
    20701 catgtgccag ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg
    20761 aattacgtga tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc
    20821 ttgtttatct ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc
    20881 aaaaaagctc attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt
    20941 tacatggcct ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta
    21001 ctaacacctg tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag
    21061 aagtaagaaa gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga
    21121 aaattgcttc ttaaattttt gtgaaatttc ggcttatttc ttggagataa aagcttctgt
    21181 cccagcctct tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat
    21241 aggaaaaaat ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa
    21301 aaaagacacc ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct
    21361 ttgacaaaac tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca
    21421 agataccaaa agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa
    21481 aagcttcagg gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga
    21541 gcaaaaagat attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac
    21601 gatgatggtt cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg
    21661 tatttcggat gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata
    21721 caagaactct caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg
    21781 tcacgatgat tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat
    21841 aaccatcgtt ttatataggg aattttctga aaattgttgc atatttttcg aaattttgtt
    21901 acttttaaaa aaaccgtaaa ggagttatgt atgacaaaaa aattatcatt taaagactat
    21961 ctttttatag gctccatgct tttcggtcta ttttttggtg ctggaaatct tatctttccc
    22021 gttcatttgg gtcaagaagc aggcgctgct accttttggg cgaatctagg tttcttggtt
    22081 accggtatcg gtcttccctt cttaggggtg attgccattg gtgtatcgaa aagctcaggt
    22141 gtatatgaat tagcaaaacg gataaatcaa acatatgcga tggtttttac catcttgctt
    22201 tacttagtta tcggaccgtt ttttgcttta cctcgccttg caaccacatc tttcgagatt
    22261 ggtttagcac catttatcga tcctagcaga cagacgctat atctagcaat tttcagtatt
    22321 gtattttttg tgctggcttg gtggttttca agaaaaccga caaagatatt agattatgtt
    22381 ggaaagttcc tgaatcctgc atttttggtt ttattaggtg ctttgcttct ccttgctttt
    22441 ttgaatccac taggttctgt aaatcaagcg cctattcagc caaattatca agagcatgct
    22501 tttttcacag ggtttactca aggatataac acattagatg cgcttgctgc tttagcattt
    22561 gggatcatca ttgtcacaac aatccaaaac atgggtgtaa caaaacctgc tgaaatcgct
    22621 aaagacacga tcaaatctgg tgcgatcagc attatattga tgggaatcat ttatactttg
    22681 ctcgcttatt taggtgctat gagcttaggt agctttgctc taagtgaaaa tggcgggatc
    22741 acgctggctc agattgctga tcattattta ggtacttatg gcagtatctt attagcattt
    22801 atcgttatat tagcttgctt gaagaccggt attggtttga ttacagcttt tgcagaaaca
    22861 ttcacagatt tgtttccgca aagaaattac gcattctttg ttgcacttgc tagtatcctt
    22921 ccttgtttag cggcaaacgt aggcttgacg aatatcattc aattttcgtt gcctgttttg
    22981 atgttcatct atccattagc aatgacactg attttactag tgttagttgg tcctttgttt
    23041 aagcagcgta cggctgttta tcgaatgact acttatttta cattgattgc ttcgattttt
    23101 gatgggttga acgcttgccc agaaactatc aaacaaacac ctattgttca aaatctttta
    23161 tatgttgcgg aaagctactt gccgtttttc aaattgggga tggggtggat cgtaccagca
    23221 gttatcggtt ttgttatcgg actgatttgg agtttcgcta agaaagaaga agtagcagat
    23281 tgataaaaga ggttgtgaaa gagacgttca gccttgagca ataaaaagta attttgaaaa
    23341 atggcttctc acatttttgc ataaatttaa cttaataccg agaaactgac ttttgaacac
    23401 cgtttattga gaggtcgtga aaaagccgct tagtttcaag tattaagaaa tattttgtga
    23461 aaatggcttc tcacattttt gcataaatt
//
LOCUS       DACDYI010000053        23339 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.053, whole genome shotgun sequence.
ACCESSION   DACDYI010000053 DACDYI010000000
VERSION     DACDYI010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.053"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(83..766)
                     /locus_tag="IWM52_001913"
     CDS             complement(83..766)
                     /locus_tag="IWM52_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP9974160.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(933..1820)
                     /locus_tag="IWM52_001914"
     CDS             complement(933..1820)
                     /locus_tag="IWM52_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9974161.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(1817..2686)
                     /locus_tag="IWM52_001915"
     CDS             complement(1817..2686)
                     /locus_tag="IWM52_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9974162.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(2823..4109)
                     /locus_tag="IWM52_001916"
     CDS             complement(2823..4109)
                     /locus_tag="IWM52_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9974163.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            4382..4960
                     /locus_tag="IWM52_001917"
     CDS             4382..4960
                     /locus_tag="IWM52_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP9974164.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            4960..5889
                     /locus_tag="IWM52_001918"
     CDS             4960..5889
                     /locus_tag="IWM52_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9974165.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            5886..7622
                     /locus_tag="IWM52_001919"
     CDS             5886..7622
                     /locus_tag="IWM52_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9974166.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(7685..8521)
                     /gene="pheA"
                     /locus_tag="IWM52_001920"
     CDS             complement(7685..8521)
                     /gene="pheA"
                     /locus_tag="IWM52_001920"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAP9974167.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(8518..9027)
                     /locus_tag="IWM52_001921"
     CDS             complement(8518..9027)
                     /locus_tag="IWM52_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAP9974168.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(9020..10315)
                     /gene="aroA"
                     /locus_tag="IWM52_001922"
     CDS             complement(9020..10315)
                     /gene="aroA"
                     /locus_tag="IWM52_001922"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP9974169.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(10328..11419)
                     /locus_tag="IWM52_001923"
     CDS             complement(10328..11419)
                     /locus_tag="IWM52_001923"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAP9974170.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(11436..12602)
                     /gene="aroC"
                     /locus_tag="IWM52_001924"
     CDS             complement(11436..12602)
                     /gene="aroC"
                     /locus_tag="IWM52_001924"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAP9974171.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(12604..13668)
                     /gene="aroB"
                     /locus_tag="IWM52_001925"
     CDS             complement(12604..13668)
                     /gene="aroB"
                     /locus_tag="IWM52_001925"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAP9974172.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(13701..14723)
                     /gene="aroF"
                     /locus_tag="IWM52_001926"
     CDS             complement(13701..14723)
                     /gene="aroF"
                     /locus_tag="IWM52_001926"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAP9974173.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(15382..16356)
                     /locus_tag="IWM52_001927"
     CDS             complement(15382..16356)
                     /locus_tag="IWM52_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAP9974174.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(16337..18220)
                     /locus_tag="IWM52_001928"
     CDS             complement(16337..18220)
                     /locus_tag="IWM52_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAP9974175.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(18238..20463)
                     /locus_tag="IWM52_001929"
     CDS             complement(18238..20463)
                     /locus_tag="IWM52_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP9974176.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            20578..21612
                     /locus_tag="IWM52_001930"
     CDS             20578..21612
                     /locus_tag="IWM52_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9974177.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(21679..22692)
                     /locus_tag="IWM52_001931"
     CDS             complement(21679..22692)
                     /locus_tag="IWM52_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9974178.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(22770..22958)
                     /locus_tag="IWM52_001932"
     CDS             complement(22770..22958)
                     /locus_tag="IWM52_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAP9974179.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         6676 a         5049 c         4217 g         7397 t
ORIGIN      
        1 aataggatta cgataggtgc cactgccaaa atgacttcca tggtaacgat ttcgattcca
       61 tctatcactt gctgccagaa actcattgga agatcacccc caaaatcatc acggcaagaa
      121 tcggccctaa cgaagcaata ccgaccattc caaaactgtc attttcattt ttcttttgtt
      181 tgatcatact agtcatccct ccggcaaggg ccaagataaa tggaacagtt actggaccag
      241 tagtcactcc ccccgaatca aaagcaatcg gcataaattc ggaactggtg aaaaatgagg
      301 cgatcaaaac gcctatgtag cctatagcca tcaattgata ataggatagc ttgaatacga
      361 cccgaagcag cgcaaaggct agaaatactc cagtgccgac gctgacgatc ccaatcagca
      421 acactctgcc aatctctccg tcagatatat catatacttg ttgtcccaaa acttgcacgg
      481 aaggttcagc aatcgtgatc acgatcccga tagcaaatcc caaactgacc aaaacggtca
      541 aacttttctt tttgatcata tatttcccta ctagatctcc gacttccatc atagaataat
      601 ctgctccaaa caaaaagagc gtcattccga tcatcatgat tgccgcacca accagaaatg
      661 aagcgaggtc ttctccttct aacggtgcaa aaacaaacgt taaaatgaca atcaggacag
      721 tcatcggcaa tatagcagca atcacttctt taaaattttt tagcatgtat cctcctcctt
      781 tactctttaa gtttacctta aaatgaaaga gttttcgagc acaaacttaa aacgttatca
      841 tttaattttt ctcaacataa aataacacac gtcccaaaag tatcttttta cttggaaaaa
      901 tgactttgaa ggaacgcgct tattgtgttt ttttatttta cagagccgat cattccttga
      961 acgaattgct tttgcaaaac aaaaaatacg atggcagttg gtaaagtagc gatgactaat
     1021 ccagccatga tcattccata gtctggtgta taggaagcac ccatagcaga taaaacaagc
     1081 ggaaccgtcc gtttatcagg agattgcaaa gctactaagg gccataaata attgttccag
     1141 ctactcatga atgtgatgat tgcagccgcg gcataggtat ttttagccgt cggcatatac
     1201 actctaaaaa aaatacttag ttcttttaga ccgtctaatc gagcggcttc tactagatct
     1261 tttggaaaag atttaacatt ttgtcgaaag aagaagatca aaaaagcagt gctaacagat
     1321 ggcaggatga caaccaagaa actgttgatt cccaaaaccg ttccgttcaa ttgtgaaaac
     1381 attcgatata aagggatcat caatgccgca aaaggaacca tcatcgatag cagtagaata
     1441 ttgaaaatta ggtcttttcg tttactttga taaatctcaa atccatatcc tgccattgac
     1501 gaaatcagca atgcaaacac agtggtcacc aaagcgatca tcgctgaatt ccaaagggaa
     1561 cgagtaaaat tcagctcatt ggaaaacaaa ttttgaaaat tgatgatcaa gttactgcca
     1621 atctttattt ttcccgatgt gatatccacg ggtgtattcg tcattcccaa aaccatccag
     1681 acaaaaggga aaatggaaat aaatgatagg atgcttaatg ctgtatactt taatactgac
     1741 ccaattgtcg aacgaagttc taatgcttgt tttttgacgt tggggcgttc gattgtttct
     1801 ttttctacca tatttttcat cgactttctc ctcctacttt cgtttggatt atcgacaata
     1861 tcacaataaa ggaaacaata acaaaggaaa ctgctgccgc atatccaaaa ttaggcgtga
     1921 atttataact caaattatag atgtattgag atagggtggt cgttgcattt gctggtcccc
     1981 cgccagtaat attttgtact tcatcaaaaa gctgcaatgt cccgatcgtc gaagtaatgg
     2041 aagtaaacaa aatgattggt tttaaatttg ggatcgtcac tttgataaac tgctgccatt
     2101 tagaagcacc gtcaatagat gctgcttcat aaatctccga atcaatattt tgcatgcctg
     2161 aaaggaaaaa aatcatattg tatccagtcc aacgccaagt gatcgaaaga atgatcagaa
     2221 ctctcgccca aattggatga gtcaaccagc caatcggtgt gtcaatcata ccaatgccca
     2281 acaacaagtt gtttacaagg ccagattgag aaaatagact tttcatgatc agagaataac
     2341 tgaccatcga agtgatacat ggaagaaaga tagcggttcg aaacaaccct ttatatttca
     2401 actttttatc atttaaaagg ttggaaataa aaagtgctag aaacaacatg atcggtactt
     2461 gtacaagtaa atacaggaat gtattaaaaa aagctttttt aaaagttgca tctgtcaaca
     2521 tccgctgata attacctaac ccgttaaatg tcatattgac agggggacca ctttgaaaag
     2581 acgtgatcaa agccaaaacc atcggaataa aaaccatgat tgatatcaaa atgactggca
     2641 agattaaaaa agcatgcccg tttttgaata aatctttctg cttcattctt ttccctccaa
     2701 ttctatcatt gattatctac atgaaaagac aagaaagaca atatctgtaa ggcagacgtg
     2761 atcttttttg tcttttcgtt tgtctgatga aaaagcgaag aaattcactt ttttaccacc
     2821 gatcaattag ccaattgtgc ttctacttgt ttttgtgtat ctgctagtac cttgtctgtc
     2881 tcttctccat tgatgattcg atgaagagct tcagctacga ctgcttcaat tgcataggtc
     2941 tcatgtccgt aattcacttc tggtatttca gatgtccatg ttgaaaaatc gtcaaagatc
     3001 ttttgaccac tgtaaaattc agaagcttct tgataagctt cttgttcatt cgcgctaagc
     3061 atcgtagata ctagtccgat ttctttcgca agagtgccca tcagttcttc attcgaagca
     3121 aacgttgctt ctaggaaatc tttcgctgaa tcttctccag ctactccttt gatcacatac
     3181 cagccagcgc cgccaaggtt agaagcttgc gctttttgca gattttctgg aagtgcaggt
     3241 attggtgcaa tcttccattt tccagattgg ttttctgctc cttggatcgt tgaagaatac
     3301 caagcgccag ttgggctaga agctactgct cctgattgta tcgcagttac tccagcattc
     3361 cagtcagagg tttgttcgac tagatcttct ttcaaaagag tggcaaacaa ttcaagacca
     3421 tactttaaag atgcattgtc ttggatcgtg actgtctccc catcttcaga tgtataccat
     3481 tctcctgcct gttgcataat catccttact cgaccaaggt cagaaggatt gacttcagcg
     3541 attttctttc ccgtcttttc tttgacatct cgcgctactt gaatatactc gtcccacttc
     3601 agattctcca tatcctcttc tgtataacct gcttctgcca tcagatccgt acgatagaag
     3661 tttgctgtta cgcccgaatc gaaaggaaca ccgtatattt tatctcctac tttgtttacc
     3721 gcaaatttat acggtgcaaa atgatcttcc tttacaatag cagacaaatt agcgaatgca
     3781 tctggataag aagtcaaata gccttgtatc ctataatctt caatcaagac gatattgggc
     3841 aatccttctg tattcccgct cgctaaagcg gtgttcagtt tctgaacgat atcatcctga
     3901 gacatcgtaa caacttctat ctctgtttct tcgttctcat aaactttttt tgcttcattc
     3961 actgctttga tattgaatgt ctcatcccac gcccagacag tgactttttc ttctttgtct
     4021 gctgtactac tagcacttcc gcatcctgtt aagaaacctg atgctgccgc taaacaaacg
     4081 actccagcaa ttaactttct gatgttcatt tttatccgct ccttttccat ccattagttg
     4141 tttacgtttg ctacattcat cataccaaaa tttgaaaacg gtttctttat tattcagata
     4201 cttcaaaaat ttctcctaaa ataataaaaa aatacaaatc aactatgaca aaaggaataa
     4261 tcttagcatc tagaccaaag atcgaaatgg tgaggtttct ttaaaataaa aatatccttg
     4321 cattttctcc tagaaataag tatttttaaa ttacaagact attcttaagg cgggtaaaaa
     4381 aatgccaata cgcgtaattc ttatcgatga tgaacagacg attttagatg gaatgaaaca
     4441 gttgatcaat tggtctgagc acgggtttca attaataggg actttttcca gtccgctgaa
     4501 agcgcttgat ttttctttgg tacatgcaat cgatatcgtg gtcacagatg taatgatgcc
     4561 tgaattaaat ggaatcgaat tatcgcggct attaaaacag caacaacctg aaacacagat
     4621 ccttatcctc tctagctatg atgaatttga tatggttaaa gattctttta aagaaggcgt
     4681 cgctgattat cttttaaagc ctcgattgag tcctgatttt tttctaaatt ccttaactgc
     4741 cgtttcacaa aaaatcaaaa aaaagcccaa caaaagccca atcaccaatc gttatgagca
     4801 gatctgcgag aatctttcac agcagattgc tggacaaact cgtccattgc ttgaccctct
     4861 tctttttgaa cacaccttgt ttttctgtta tataccgaaa aacggccatt cagttcaaaa
     4921 caaggctcta aacaaaaaca gtcgatcacc cattcattga tggatagcga cgattcgttt
     4981 actatttatc ctgtttctac cttttcggaa gaatccggct atgtaatcaa tactgattcc
     5041 aaagatacac ttaggaagtt tgttcaaaag tttcgtcaag aagtttttcc aggtgatttt
     5101 tttgttgttt ctgattcttt tgcaatccaa gatatcaatg aaatattcca agagatcaga
     5161 aattgcacca aaggacaacg tttctatcat aagggaaaga accttgctta tcagcatgaa
     5221 cttcttccgt tattaaccaa ccacgagcag caagcaagta gttatttaac aaaaatcgtt
     5281 cataaagact acgctttagc tgtcaaagaa cttgagaatt tcttcacgac gatattggaa
     5341 cagcctattg atccagctta tttgaaacat gaggcaatca atcgtttcta tgcattactc
     5401 aacgctttgg ctgacgagca tgttgggaat gaagaacttt ctttgctcaa gatcgccctt
     5461 ccttctttat tagccgatgc tgagtatttc gaagattttt ctattcttgt caaagaaaca
     5521 cttgagaaac tcacccactt ttctgcaaat gcaaaaaaag acaatttcga tatcttgact
     5581 ttgatctacg agttcgtcca ggaaaattat gagaaagaaa tcaatttaca acttttatcc
     5641 gaaaaatatc atttttcata tagctatctc tcagccatct ttactgaaaa atttggtatc
     5701 aacttttcaa aatatctaaa aaaagtccgt atcagtaaag ccaagctctt tttgacggaa
     5761 acacggctta ctttgacgga aatctgtgag aaagtcggct ataccgaact gggttatttc
     5821 tccagagttt ttaaagaaga gacaggaatg acaccttctc aataccgtaa aggaactttt
     5881 gtaaaatgag aaaaaaaatc aaagagcatg aactctttgc caaacttctg ttcattttgt
     5941 tcatcggtct ttttctgcaa gccatcgtca tttctttgtt tatttatcat cgatcaaggg
     6001 atgcctatat ccagctgttc aatcaatcga atgatgtggt actaaaaaag attcaatctg
     6061 aatttgaaag tttgaatgat acaatcgaga atacattagc cgcttttgat tctaatccag
     6121 ctgtcaaaag ctatttttcc aatgatccag cccaacatat ggaacagctt cagcaactaa
     6181 gaacaatcca aaaaatgaat gactctcttt caaaaatcca tccaatgatc gattacgatg
     6241 tattgatctt tggagaaaat ggaagaactt ttgtcggaaa cgacatgctc actgccgtat
     6301 ctgccgattc tttttttcaa tctgcgattg ctcagagagt gaatgaacgt gctgctgata
     6361 cacaaatgct ttttgcacac cacggattga ctcttcgcga taagaaagca cccagcgtct
     6421 tctttgtcaa aaaactgaaa aatacattga atcacgttta cggatatgct gttttatcga
     6481 tctcatccaa gcaactagcg aatctttttc aatctgtagt aaatcctgag atatcgaaga
     6541 tctccttcat gaacgaagaa cagacgatca tcgcatcaaa tgaagaaaat acaattggga
     6601 gccgatcaat gattttcaaa aaactaacag taggtgaaac aacgatttca aatggacagc
     6661 agttgacccg tcttcctctg taccgccagg attgtacatt actcagtcaa gtcgatcttc
     6721 attctttatc taaccaaatg ggcgtgattt ttccgattat tttgtataat atcgtgacac
     6781 ttgcttttgc tggctcattg gtatttatgt atttggatcg acacacaaaa tcgatctacc
     6841 gcttgattga ttcgctaaaa cggctagagg aagatggctt aaaagctact gttcctgtac
     6901 aaggaagcca tgaagtccaa ttgctaggga atacgatcaa tcaattgcta agtaatgctc
     6961 agcatcatta tgaacagacg atccaagacg aaaagaagaa acgttcgctt gaaatccaaa
     7021 ctatgcaagc acagatacag ccccatttta tttataatac gctgactagc ttgaaatttt
     7081 tgatttggca gaaaaaaaca gatgaagcag ttcaaggaat cgacagtttc attactttac
     7141 taagaagtac gattgggaaa aaagaagaag tgattccagt caaggaagaa atcaaaagcg
     7201 tacaaagtta catcgatctt ttgagtatgc gatatggcga aagaatctcg gctaaaataa
     7261 tgattcctga tgaactgcag tcattatcta tgccgaatat gatccttcaa ccaattatcg
     7321 aaaacgccta tcttcatgct tttcacaata aaacgcaagg attcatcacg gtgtacggca
     7381 aaacaaaaaa tcagcttctt ttattcgaag tgattgataa cggcgatggc ttcgatactg
     7441 aaaataccaa acaaaaaaat gatcattttt caggaatcgg catcagccat gtagatgagc
     7501 ggatacgcct aatgtatggc gatgcatatg gcttaacgat ccaatcagtg attgggacag
     7561 gaacgactgt aacgatttgt ctaccgcttc ttcctaaaaa tcaatcagaa tatcaaaaat
     7621 aaagaatagc caagcgctga tttttttatt ttaagttaaa taaatcaatc agtattcttg
     7681 actttcatct acgcgtctct tctactgtca gcactggata acagcccaat atttgacttt
     7741 ctcctccgag catcttgatt tcttggagcg cattctctac taatgtcatt ggacggtcca
     7801 acaacaaatc gatcacgaaa aaatattctc ccaagctagt tttcaatggt cgggattcaa
     7861 tcttacttaa gttgatttcc cgccagccaa aagctgccag cattttatga agcatccctg
     7921 gccgattagc aggtagtgtc aagatgacac tcatttttac tggagctgac tgctggctgg
     7981 tcatctttcg gtcaccgacg atccaaaaac gtgtctggtt caactcatta tcttggatat
     8041 tctcagctaa gatctccaat ccaacatgct gtgctgtttc taatgaagca atcgctgctg
     8101 cgtcttcttt cggatgttct gctacataca ttgctgcagc agttgttgat tctgttgcta
     8161 ctaaaggaac attaggataa tgcgtttcta aaaactgttg tgattgtgct agtgcttgtg
     8221 gatgcgaaag gatttttgta atctttgttg ctggattgcc taaaagctgc tgcttgatag
     8281 gaagaacgat ttcactttta acttcgactt ctggatgttt ggaaagaaga tcgattgtgt
     8341 gatgaacagc tccttccaat gaattctcta caggaacgac agccagatcg acttgtttgc
     8401 gaaacaaagc gcgcaaacaa acagggatgg aagcatacgc agtcaattgt tctgtaggaa
     8461 atagctgaca agccgcctga taagtgaatg aactttctgg ccctagatag ctgacgttca
     8521 tatgcttttg atcctttcta taatggtatc gatgatcttt tctggtgttt gattggtcgt
     8581 gttgattcga tgtgtcgcac tttcttcata ccaagaaagt cgttgtgcca ataatgcttt
     8641 tatttcttct cttgtttttc caagagctaa aggtcgaata gacgtttcat cttgttggat
     8701 acgatcaact agacagtccg cttctgcctc taagtaaaga acaatcggct gtgtttttaa
     8761 aaaacgccga ttttctgcac tttggacaat ccctccacca gtcgcgataa ttttgtcttc
     8821 atttgacagc tttcttaaaa tatctgactc cacttttcga aagctttttt caccgtaata
     8881 gtgaaaatat tcggcaatcg acaattggat atgttcctca atctttgtat ctaaatctac
     8941 atactctttt tgtaaacatt gagctaaccc tttaccgatt gttgtttttc cagcgcccat
     9001 gaaaccaatc agcaaaatgc tagccatgag cttctcctcc tgacacaagt tctgctaaat
     9061 cgtcaaaaaa tgccggataa gagattgaaa cagcttcagc tccttctagt tcgcaaggtc
     9121 catcagctat caaagcagct acttgcagca tcataccgat tcgatgatcg cctctgctgg
     9181 acacttttgc gccatgcaga ggtgtcggcc catggatgat cagaccgtct gctgtcgctt
     9241 ttatctttgc tcccattttc tgtaattctt ctgccaccgc atcaattctg ttggtttctt
     9301 ttacttttaa ttcttctgcg tctttgatga tcgtgattcc ttctgcttga gtagccacta
     9361 aagctagaat gggcaattca tcaatcagac gcggaatgat ttctccacca atcgttgctt
     9421 tcttcaaatg actcgtcttg acacttaaat cagccgattg gttctgttca ttgtgattcg
     9481 tctgggtaat cgctgcgccc atctcctcca acacatctaa gataccagtt cttgttggat
     9541 tgaccccgac attttttaac aacagttggc tttccggaac aagaagtcct gctgctagaa
     9601 agaaagcagc tgatgaaata tcacctggaa cagttatctg ttgaccagtt aatttttgcg
     9661 gtccggtcac tatgatcgtt ttatcttcca ctgtgatcct tccgccaaat tgacgaatca
     9721 tttcttccgt atggttacgg gaacgttctt tttcaacgat tacggatgtg ccttctgctt
     9781 gtaatgccgc aaacagcaaa gcagatttga cttgcgcact ggctacaggc atattgtatc
     9841 ggataggtga taaagagcgg gttcctgtca cagtgattgg tagatactgg tcattttctt
     9901 ctccttgcaa gtcagcgccc atctcgcgta aaggttccat cactcgcccc attggacgtt
     9961 tagacaacga agcatccccg aacagagtag tcgtaaaagg ctgtccagcc aaaataccca
    10021 tcaataaacg tatggtcgta ccggagtttc ccatgtcaat tgattcggtt ggttggacaa
    10081 atgagtggct accgcgacca tgaatcttga tcacctcttc ctcttcttcg atctcgacac
    10141 ctaattgttg gaaggcatgt aaagtactca gacaatcttc tgcgcgcaaa aaattttgta
    10201 tagtcgtcgt tccttcagca attgctccaa acatgatgct gcgatgggaa attgatttat
    10261 ctgctggtat cctaacagtc cctcttaatc catgtatttg ttgtaataat tgcatttatc
    10321 cagcctccta tttgatccag caatgataac tggtattaga cataatgatt ctttttgctt
    10381 gtaacaaatc ctgttggttt ttaaaactga tttgcagtat tcctaaaata tcttccctgg
    10441 tctcttggat cttcaaatta atcaaagaaa ctcccgcttt tccgatcagt ccagttactt
    10501 ctgcaataac gccaggttca tctggcacat cgataaataa atcaaaaaag gcaggaatcg
    10561 cgccttgctt atggacaggc aactggtctc gactttcttt tgcccgattg aaaaaagaaa
    10621 agatttccga ttggttctca gtcatgatcc attctgctat ttgtgtcatt tcttgttgcc
    10681 aatctgccaa tcgttccaat aatatctgct tattgctcaa caaaatatct gtccacatga
    10741 caggatctga ggaagcgatc ctagtgatat ctcgaaagcc tcctgctgcc agttgtttgg
    10801 ctcgcggata ctcttgattg aattgttctg cctgattgac taagccagct gccagaatat
    10861 gtggaaaatg acttagcatt cctgttatac ggtcatgttc tcttgctgta agagttacat
    10921 attttgcttt ggttccgcaa aatagttctt gcagtttttc aacatctttt ttcgtctttt
    10981 ccaaaggtgt aaagatgtaa taggcatttt caaatagttt agcatctgct gcctgtatac
    11041 ctgatttatg agaaccagcc attggatgtc caccgacaaa ccgaagattt ttctgctcag
    11101 cggatgtcac gacttgctgc ttcgtactgc tgacatccgt aatgatgacg tgatcagaca
    11161 agttgattct ttcaaggaca tcaagataag ctaaggttgt acggacggga acggctaata
    11221 agataacgtc tgcttgttct gcacctgttt caaaatcact tgcaacgtaa tcaacgatct
    11281 gctgttctac tgcttgctca cgggtttttg actgactatc ccaaccaact actatcagtt
    11341 ctggattggt ctgacgaata caaagtgcca tagaagcacc aatcagtcca agccccacga
    11401 taaaaacatg ttgtgccatc ttatttgccc cctttttagt aatgtttcaa gtagtcacgg
    11461 tactccttca ctgcagcttt caactcaaca agcgaatcac ttgggaattt tgacaatagc
    11521 tctgtcgcta ccacagtggc tacaacattt tccgcaacga cgctagctgc aggaactgct
    11581 gtactatccg aacgttctac acttgctttg taaggttctt ttgtatcgat atcaacactt
    11641 tgaagtggct tgtacaatgt aggaatgggt ttcatgaccc cgcgaatgac aagtggttcg
    11701 ccattggtca ttccgccttc gaagccgcct agattattgg tacgtcggaa atagcctctt
    11761 tcttcttccc ataggatctc atccatcact tctgagcctg gtaatgaacc agaagtgaat
    11821 ccgaccccaa attctacgcc tttaaaggca ttgatactaa ccacagcttg tgcgattttg
    11881 gcatctagct tcgtgtccca ttgtacgtag cttcctaatc cagctggaac gcctccaacg
    11941 agcacttcta caattcctcc gatcgtatct ccatttttct ttgtctgatc gatcaaatct
    12001 tttattcttt gctcaactgc tggatcaaca actcgtactt ctgacgcttc agaaatttct
    12061 ttgatttctt gaatcgagat atttccaggt atttctgctt ggattccacc aagacaaatc
    12121 acgtgtcctg ctacgatgat gtcaagttct tccagaattt tcttggcaat tgcgccgatt
    12181 gctacacgca tggtcgtctc tcttgcagag gaccgttcaa gcacattccg aagatctcga
    12241 tgatggtatt tcataccgcc taccaaatcc gcgtgtcctg gacgcggacg tgcaactcgc
    12301 cgcagtgatt ggtctttttc aggcacttct gctacagaca tgacagttgt ccaatttttc
    12361 caatctttat tttctaccac taaagtgaca ggtgaaccaa ttgttttgcc gtgacggatg
    12421 ccagacgtga tccgaacacg atctttttcg atcttcatcc ttcctcctct gccgtaacca
    12481 gcttgtctgc gagcgagttc gtgattgatt tcttcttctg acagaggtaa tcctgctggt
    12541 agtccttcaa taatggctgt taattctggt ccatgggatt ctcccgcagt catataacgc
    12601 atcttacagt cctcctaagt aatctttgaa tgtctctgtg ggaacaggaa cgatttttgc
    12661 ttgcccaatc ttttctagca agatgatatt caattggctt cctcttgctt tcttatcatg
    12721 ggttatcgcg tggaataatt catctggatg acttagatcg tacgtaatcg gcagatggaa
    12781 tttttgaagt atcctttcta aagcagctgt cgatccagtt ggagacaagc cgattttttc
    12841 tgcatgagaa gtgatggcta ccattccaat agctactcct tctccatgac tgactacccc
    12901 atatcctgct gtattttcaa ttgcatggcc aattgtatga ccaaaattta aaataagtcg
    12961 atttccttgg tcaaagacat cttcttccac tacttttcgt ttgatttcca acgtagcagt
    13021 aatgattgga acagcatgtt ccagaagatc tttttcgtct ttcagctcgt tcagctgttt
    13081 ccaaagcgtt gcatctgcta tagctgctga tttgatgatt tcagcgattc cttctcgtaa
    13141 acgacgtacc ggcaaagttt ttagaacctc aggatcaatc aatacaccag ctggctggct
    13201 aaatgttccg acaaggtttt tcgctttgtt tgtattgact gctgtcttcc cgccaatact
    13261 agaatctact tgtgcaagta gcgtagtcgg aatttgaacg aaatggatcc cacgcatgaa
    13321 cgtcgaggcc acgaatgctg ctagatcacc caccattcct ccaccaagtg caagtatccc
    13381 atcgctgcgg gtaaaatcat tatctgctaa aaaatcgtat aactcagctg cttgttctaa
    13441 agatttactg gtctcacccg ccggtacgat tgaaaggact ggttcaaaat tagcttgtct
    13501 caattggaac atgatttttt ctgcatatag tggagcaacg tttgtatcag tgatcacagc
    13561 tactttttga ggtctccaaa tcgtttgaag ccatgatcca gcctgttcta gtgcgccttt
    13621 ttcaatatga atatcgtatt tttggttatc aacatgtaca gtaagcattc gtttcacttc
    13681 tttcttttaa aaagctggaa ttaatcgagt gtctgaatct tttccattgc ttctttcatg
    13741 atcgcaactt ctttcatcat gcgtgagtaa gtcttttcat tcaacgattg gggaccgtct
    13801 gaccaagcat ttgcaggatc aggatggatc tctacgatca tgccatctgc tccgcttgcc
    13861 acacccgcac gagccattgg tggtacaagt tcccaaattc ctgtaccatg acttggatca
    13921 acaatcacag ggaaatgagt taattttttc atcaatggga cagcacttaa atcaaacgta
    13981 ttacgtgtcg ctgtttcgta agtccgaatc ccacgttcga taaaaatgac aggagatttg
    14041 tcttcgactg caatgtattc tgcagcatta agccattcat tgatcgtacc ggaaattcca
    14101 cgtttcaatc caacaggttt acctgtttta ccgacagcag atagcagttt aaaattttgc
    14161 atattacgtg cgccaatctg taggatatcg ctgtactcag ccaccatatc gatatgccct
    14221 tcatccatca cttcagtaat cactttcatt ccaaattcat cagccgcttg acggatgtat
    14281 ttcaatcctt cttcttctaa accttggaaa gcatatggag aagtacgtgg tttgaaggca
    14341 ccacctcgca gaatttttgc gcccccggct ttggccattc gcgcacattc tctgatttgg
    14401 tctaatcctt cgatagaaca aggaccagcc atcgtcacga aactgccgtc accaattttt
    14461 actccgtcta catccacgac tgtattgcca ggatggaatt ctcgagaagt caatttgtat
    14521 gtgtttaaaa tacgcacagc attttctact ccttcatagc gtgtaaatgc tccttcttgt
    14581 aaacttcgcg tatcaccttt tagtccaatc actactcgtt ctgttccttg atcgattgag
    14641 actttgaatc cttctttttc gatatgatcg acgacttttt ttatttgttc ttccgttgct
    14701 tcttttttca taattactat cattgttttt cccgctttct tttgtttact tatctatctt
    14761 ggctgatttt ctctactttt tcaaggtggt tttacattaa ttcctactaa atgaccgtgt
    14821 cctgttgttc tcattttcgc tccttctttc ctacttatga cccagataat caacaaaaaa
    14881 agatgtccct gtaggaaatt gcctctacag tgaacatctt caacgagcct taaaaagcgc
    14941 tgcaaaaata taaaatagcc actatagatg aatttctcta cgtggctgta agtcgtgttt
    15001 aaaaatcttt agccatcata gatacaaacc tggtttgttt gtacccacga agtcatgaat
    15061 acaaacctat ctaaaatagc taaagtaata cgtattcagt tttgtaagtt ttctagattt
    15121 cataaaaaat cgctccaaac tgatgtaaga attgtaccta gtatagacat aaaaaaatcg
    15181 actgtcaatc atttttatga aacttttttt aattttctga tagttaactt tgtttttcag
    15241 aaaaataaca attggtttta ttgaagaaaa tagtcatgaa caaaagtatc tgagaacttt
    15301 tttactctct tgttcatgac atatagaata aagcagcttt gtcttgtaaa ctccgttttt
    15361 caaataataa tctaaagatt gctactgaat aataaagccg atttcatggt cctcttcgct
    15421 gctgattcgg taaggtgact caacatccgc tccccagcta ttgatacccc cgactcctct
    15481 tacttttgca aaaatcgtaa gaaccgttcg tctttctggc ggtagttctt ctatatgtgt
    15541 tgcctgttcc agttcttctg ctttgaatgg taaagcggaa aaagcaaatg gcgtatcctg
    15601 ctgttcaaat cgcaacgaaa aatcttcttg atctttatcc gtattatcca acgtttgatt
    15661 tcttgttagt tctacccatt tcgtttccat atgcatgccg cattcttggg gaacaaggta
    15721 tggggaaacg gggagtcctg ccactttata aataccttcg acaccaccag ccatccgatc
    15781 cggatatgtt tcccctgata ggccttcata ctcaaaaaat tttgctttcg ttggcataat
    15841 gaaccttaaa cccaatgcgg gtaattccgg taatcctttt tgtccataat aatgcatatt
    15901 gacaagcatt ttaccctctc cagcgatttg gtaggaaatt gtcacttggg tttttggtac
    15961 tgtgttgatt tccagtgtaa aaatgatttc aaccgtttca gcgtattcat tacttgaata
    16021 ttgattattt actggagcaa tcggcaaatc gatcttttta ttgtccacca gtacttctat
    16081 ttctttgact tttggataca atccggctcc taaccacata ccggatttaa atccaaatcc
    16141 gctacctctg tcattatcgg tcgttgctcg ccaaaaaact ggaagtgttt cccggtatag
    16201 ccattctttt ccgtttttat tgagcgattc cagtccgcct cttgtataag aaaaaatata
    16261 atgcgtattc cccgatttga cacctaatgt acagtcgccg aagacgattg caactttttc
    16321 cgtatccata tgacttttat ttactgtatt tttcataata atatcttacc tcctgcatgg
    16381 cgggtttttc ttgtccatct gcaaaaacga tcccattccc tgagaattca tagtcacttg
    16441 gtttatcgtc aaagtcaccg ccatagcgca tcacttctct gcctgtgatt ggatcagtca
    16501 cttggatcgc ctgatcttta aaatcccata tatagcctcc ttggtacatt tcataacgat
    16561 caagcaacgt catgtaagaa tccattccac ctaacgaatt ccccatgtca tgcatatatt
    16621 cacataagat gaacggtttt tccgctttat tttcaaggta gtcaatgatc tcttctggtg
    16681 gcgcatacat gcagcttttt acatctgaaa tcgaatcttc gaatgctgga tttggaaaat
    16741 tcccttcata atggacaagt cgatcggggt ctactttttt gaagtaatcg ttcatttgtc
    16801 gaatgttttc ttccgcataa gattcatttc caagagacca aaaaagaata cttggatggt
    16861 tttttaatgt ttcgaaattc gtttttgctc gatcaagaac agccgcctgc cactgaggta
    16921 ctgcacctgg tacgttgtaa gaaggctcaa atgcgcccat tttttgccat gagccatggg
    16981 attcgaggtt cgtttccgcc atcacatgga ttcctgcttg gtcgcataaa taataccaag
    17041 tgatctggtt ggggtaatga gatgtccgta cgccgttgat ctttgcattt tttatgatag
    17101 ccaaatcttt ttccatatct tctatagtga tggcgcgtcc ttttttcgga tgccactcat
    17161 gtcgatttac cccgttgata atcatccgtt ttccattgaa gtagatgatt ttatctttga
    17221 tttcgatttt acgaaaaccg aaggaatagt caatatactc cgttactctt ttttcttcgt
    17281 ctatcgtctc caattcaagc ttatataaat tgggctgaaa atgcccccac agtgccacat
    17341 tttcaaatcc atcgatgcga atcgtcattt gctcttttaa cggatattgc tgttgataga
    17401 tttccttccc atcaggatct agtatcgcta cattcagctg actgtttttg gctacttgct
    17461 caaaagacaa atctacttca agtttgccat tttgacattt gtcatctaag cgaggtttga
    17521 tccaaagatc ttctaaatgg ttttgtcctc gagcaactaa gaagacatct ctgaagatcc
    17581 caaaaaatcg gaaaaagtcc tgatcttcca agaatgcagc tgtacttctt ttgtaaacct
    17641 ccacagctag aatattttct tcttccaaat aaggtgttaa atcaaattca gacggagtga
    17701 acgaatcttc ggcatacccg ataaaatgac cattcaacca aataaacatt gcttgttcta
    17761 caccagcaaa atgaatcgag atttgttttc cctttagacc ttcttctaat cgaaaagtcg
    17821 tcaaatacga accaaccgga ttgtctttag cagctgaaaa gatcccttcc gcctctttct
    17881 cagggcttgt cgtgaaaggg ggacggcgaa atacatgtcc ctcccacgga tacatcgtat
    17941 tgatgtaatg gatcttgtca taatttgcta attcaatatg actaggaaca gtgatttcat
    18001 caaaagaaca ggcatcaaaa tcttttttat aaaatgtttt tggtcgtacc gaaggatttg
    18061 gcgaataaca aaaacgccat gatccattta aagactgttt aagggaggat gtggtttcta
    18121 cttcttccct tgttgcataa caaacatgat cgctatgtgc atttgattgg ttcactctaa
    18181 aaacttgtgg atcatctaac cattttatat ttgctttcat acttttttcc ttctctctca
    18241 taactttttg aaatgaagca gttctgtttg gaaatcacct tgaaaatcgg ggaagtaaag
    18301 tccaactgtc atcaattcgg cccccgaaaa tatccgcttc gaggtctgtt cctgataaag
    18361 tgcctcatca tctaacccca tcagatatac ttcatggaac gctggttgag ctgaagctag
    18421 tatcctccct aaaaacacaa tagcttcatc ttgtttagga gacacaaaca tccatgcggc
    18481 aatattttct tcaaaaggac tcgccaaacg ataataatgg ccatactgga tcaccggacg
    18541 aatcgtttga taggatataa tttggtcttt tacttgcttt ttttcttcag gagacagctt
    18601 tgtcaaatcc agttcataac caaaaacagc gctcatagct gtttctcctc ttgttttcaa
    18661 cgaagtagat ctgtcggtct gatgatttgg tacagctgat acatgagctg tgatggacga
    18721 aatcggataa gcaaggcttg ttccatattg gattttcata cgctccactg catccgtgtt
    18781 atcacttgtc catgactgcg gcatataata aataaacccg gcatcaaacc gtccacctcc
    18841 accagaacag ccttcccata agatttccgg ataatcagtt gtcaaatgtt caagtagttc
    18901 ataaagcccg agcatataac gatgcgctac ttctccttgt ctctgtgggt caagggtaat
    18961 cgaatagaca tctgaaagcg aacggttcat atcccatttg atgtagtcaa gaggaatcgt
    19021 atctaaaatc tttctcattt gtttctcaat cgtctgccga acatctaacc ttgtaaaatc
    19081 cagtacatgt tgtgatctgg aagcagaagg cattctacct ggctcctgca taagaaaatc
    19141 aggatgtgtt cgatacagtt cagaatcaac cgagatcatt tcaggttcaa accataaacc
    19201 aaatttcaat cctttgctat gagcataatc tgctatttca cgtaatccat ttgtcaactt
    19261 gccttcatac tcgaaccagt ctcctaatga agaattgtcc gcatctcttt ttccaaacca
    19321 gccatcatct aatacgaaca tttcgatgcc tagctccttt gcttcatcaa tgatcgcctt
    19381 gattttttca ctattgaagt caaagtaagt ggcttcccag ttattgacca gaatcggccg
    19441 ttcagcaaat tgatgacgac ctcttgcgat tctttcacgt agcaactcat ggtggatctg
    19501 actcattttg ttcaatccct gacttgagta agaaaggatc atttcaggtg tttgaaaaga
    19561 ctggcctgca ttcaattccc acgaaaaacg atggctgttg atcccaccga cgactctcaa
    19621 ttgattgatc tgatcttttt caagttcgaa agaatggttg ccggaataaa caaattgaag
    19681 tccgatcgct tctccagtat tttctgtcgt atgatgatga actaaagcaa taaagttatt
    19741 catatggtga ctgctcgttc ctcgacggct ttcaaatact tttgttccag ataaaacaga
    19801 ttgccgtgaa atctgtcttt cattgacatg tgctcctggc aaagcaatga cttcatcaaa
    19861 tcgcctagta tgggcaaagt ctagctggaa actagccgct ttttctagtt tgattgtttc
    19921 tccggtttca tttcttatct ttacagatcg tgtgatcact gctctatcag taaatatggt
    19981 atagaacaaa tcgaaataaa tcgctgtttc tctatcctta agtgaaatca ccaacgtttg
    20041 tgcctctgac ttgtccatca cataagtttg aggcaagcct tcgatctctg gcttcccatc
    20101 tgtaatataa tggctgtcat atctcagatc cagtaagtct gatccattct tgcgctgaat
    20161 gatcgatgcg ggcgttcgaa agtccatgct gccatgagaa gagtattctt gcggtagtgt
    20221 atctttcgac agagaccggt caggatttaa gggaacattt cctgaaaatc cacgatctct
    20281 tcggggataa tttttattgt ctttgtactg cttgactggt ttgccaaaat aaatatgcgc
    20341 caatacattg ctttcttcta tctttatcag ataagaaaga aatgtatttc gtaaatggaa
    20401 gactcggctc ttttcatcaa ataagatgaa atttttatct gtttttctat gcgtaatatc
    20461 catcatttca ctcccaacaa aacggaaacc gtttactttt ttttataatt cctgtatact
    20521 gtttactaaa caattttgtc aataaaaatt ttttattttt ttgaggagtg tgtaaaaatg
    20581 gttggaatta gagatgtcgc acgaaaggca aatgtctctc ctgctacagt ttcccgtgtg
    20641 ttgaataacg atcagacgat ccatgtggct cagactactc ggcaacggat agtagaggca
    20701 gctgctgcgt tggattatga catcagtact cgtaaatata tcaaaaagag aatgccttcg
    20761 attggtgtga tctctacgat cggcaaagcg agcgaagaac aggatatcta ttacaaacat
    20821 ttgcgagtag ggatggaaga agaagctcga cgtctgcatt tagggatgaa tcgaatctat
    20881 aatctatctg ataatcctaa acaatgggaa gatctagatc agttaggtgc ggtgatcgtt
    20941 gttgggactg ttacgaaaga atcgattacc gccttgttaa aacaaaacat gcaaatagtc
    21001 gtcgtggata accctgacat ccagcaagag gtcgatatgg tgtatgcaga tcttgaacgg
    21061 atgactaaaa ccgtactgga attgttctta gaaaatggtc ataaaagaat agcctatatc
    21121 gggggatata aagtcgatat ggatgaattc ggtgggaagc agatttctat aaatgaaaag
    21181 aggatgcgtg cctatcagca ttttatgatc gatcatcact tagaaaaata tatgtcttat
    21241 catctaggtg gctggacaga aaaacaaggt gaggtacttg cgactaatct attagaaaat
    21301 gaatcccctc ttcctactgc cattcttgtc gggagcgatc caatggcaat tggtgtctat
    21361 cgggcattga aacaaaaaaa tatccaaatt ggagaagaga ttgttgtagc aagctttgat
    21421 aatatcgaga caatcgcaaa cctctcccct ggcttgacca gtgttcaaat caatgcgaaa
    21481 tcaattgggc aagcagctgt tcgaatggca ttggagcgaa ttgacggtat acgggacgaa
    21541 ccaatcattt tgacttatcc tgcacgattg attgttaggg aaagttttat cccaagaaca
    21601 aaaaggtatt gaaagagagg ttgtgaaaag ccgcttcgtt tcttcatact caaaacgaaa
    21661 cggctttttc acaacctctt acttatactt attcacaaaa tccaatgtct gctggtaaca
    21721 attttgcgat tcttttagcg tgtaatcaaa ttggtattca tgggtaatct ctttttttgt
    21781 atctgtataa aacaaggatt gcacgggtac ctttaattct gtcaaccgat tttctaatgc
    21841 ttgaccttgc tcctggaatg agtatgcatt tccgtcagtt atatacgttg gcggaaattc
    21901 cttagaaaca tggtcagcca cactagcttc tttcaattgc tcactcgttt tccaatcttt
    21961 tgttccaatc aacgaccacg caacggtttt gacgaaaaac ttcataaatc gatcatccga
    22021 atgctgctgt gctgttagct tcaaatcaac aggtccgcaa taagaaatgg ttcctttcag
    22081 actatctttt ggaagtagct gtttgatttt catttgttta gcatacgcat cattcgtttg
    22141 tatagctgca tattgcaatg cgatttgtga acctgcacta tctccaccaa aaaataattg
    22201 ttccatgtct agcaatttgt cgtctttttc ttcagctaat aaatctttga tcagttcatt
    22261 cacttgaaga acttgattag ggtattctga atcaggcgct aattcataat tcatcgcaac
    22321 cactgcgatg ttggcgtctg aaactaattt tgtcgcaaat tctttgacac ccgatttatc
    22381 tccgccgacg aatcctccac catgtaccca aaatagaaca ggaacaggcc ccttgctgtt
    22441 ttttggatag tagatgtcat aagtattctc tttatacttg gactcatacg tcaaatctgt
    22501 ttttttcgcc acgttttttt ctgcttgatc gaacgttttt ttatcagtga tttgaacagt
    22561 atttgaaaac atatggttga tcaagaatgc tccaggtctg ggggaaagct ggaaggcaag
    22621 aaagattgca aacgcgatcc caagaatgat tccgaagaac catccgataa atttaagaat
    22681 tttctttttc ataaggctcc ctcagtagtt agtattaagc attctgatta taccatagtg
    22741 ttatttccta tcaagctctc ctagatgatt taatgatttt ccccaaactt cttctgcaat
    22801 gcttttgaca aaagaaattt tttccattgt tcttcaaaca aaacattttc ttcttctgtt
    22861 gaagcaaatc cacattgagg acttaagcac aaacgatcca aaggcagata tcgtgtcgct
    22921 tcgtagatcc ggtcaatcac ttgctggtgc tcttccattt cgccattttt cgttacagtc
    22981 aaaatctaag acaactaatt tatcttcaga gactttctct aaaagaacaa aactcccagc
    23041 acgttcacta tcgtattcta aataataggc acggacattt tcttgagtaa aataatgggt
    23101 ctgctacgtc gtcatccccc ctgatgccgc caaaatcgaa taataatttc cgcgtcaaac
    23161 atgtatattt agtgttgaat catttgagaa tatgaaaata aaaatctttt tgatcaatca
    23221 gttaatcaaa ttgaaataac gaaaaatctg agacagaatg acgttcattc tgacccagat
    23281 ttgattttgc acaactcaac agtccttatt ggcaaatcgc caaaataatg tagactaac
//
LOCUS       DACDYI010000054        22987 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.054, whole genome shotgun sequence.
ACCESSION   DACDYI010000054 DACDYI010000000
VERSION     DACDYI010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.054"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(105..560)
                     /locus_tag="IWM52_001933"
     CDS             complement(105..560)
                     /locus_tag="IWM52_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974180.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(714..1922)
                     /locus_tag="IWM52_001934"
     CDS             complement(714..1922)
                     /locus_tag="IWM52_001934"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP9974181.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2034..2366)
                     /locus_tag="IWM52_001935"
     CDS             complement(2034..2366)
                     /locus_tag="IWM52_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP9974182.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2514..3389
                     /locus_tag="IWM52_001936"
     CDS             2514..3389
                     /locus_tag="IWM52_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9974183.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3471..4052)
                     /locus_tag="IWM52_001937"
     CDS             complement(3471..4052)
                     /locus_tag="IWM52_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAP9974184.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4058..5317)
                     /locus_tag="IWM52_001938"
     CDS             complement(4058..5317)
                     /locus_tag="IWM52_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP9974185.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5319..6365)
                     /locus_tag="IWM52_001939"
     CDS             complement(5319..6365)
                     /locus_tag="IWM52_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974186.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6600..6875)
                     /locus_tag="IWM52_001940"
     CDS             complement(6600..6875)
                     /locus_tag="IWM52_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAP9974187.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7118..8428)
                     /locus_tag="IWM52_001941"
     CDS             complement(7118..8428)
                     /locus_tag="IWM52_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAP9974188.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8610..9839)
                     /gene="rpsA"
                     /locus_tag="IWM52_001942"
     CDS             complement(8610..9839)
                     /gene="rpsA"
                     /locus_tag="IWM52_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAP9974189.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9974..10654)
                     /locus_tag="IWM52_001943"
     CDS             complement(9974..10654)
                     /locus_tag="IWM52_001943"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAP9974190.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10703..11338)
                     /locus_tag="IWM52_001944"
     CDS             complement(10703..11338)
                     /locus_tag="IWM52_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9974191.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11404..12807)
                     /locus_tag="IWM52_001945"
     CDS             complement(11404..12807)
                     /locus_tag="IWM52_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAP9974192.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12794..13825)
                     /locus_tag="IWM52_001946"
     CDS             complement(12794..13825)
                     /locus_tag="IWM52_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974193.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13856..14077
                     /locus_tag="IWM52_001947"
     CDS             13856..14077
                     /locus_tag="IWM52_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAP9974194.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14242..14979)
                     /locus_tag="IWM52_001948"
     CDS             complement(14242..14979)
                     /locus_tag="IWM52_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAP9974195.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15132..16472)
                     /locus_tag="IWM52_001949"
     CDS             complement(15132..16472)
                     /locus_tag="IWM52_001949"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAP9974196.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16709..18232)
                     /locus_tag="IWM52_001950"
     CDS             complement(16709..18232)
                     /locus_tag="IWM52_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAP9974197.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18610..19221)
                     /locus_tag="IWM52_001951"
     CDS             complement(18610..19221)
                     /locus_tag="IWM52_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9974198.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19344..19469)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19551..20267)
                     /locus_tag="IWM52_001952"
     CDS             complement(19551..20267)
                     /locus_tag="IWM52_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP9974199.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20273..20842)
                     /gene="scpB"
                     /locus_tag="IWM52_001953"
     CDS             complement(20273..20842)
                     /gene="scpB"
                     /locus_tag="IWM52_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAP9974200.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20826..21623)
                     /locus_tag="IWM52_001954"
     CDS             complement(20826..21623)
                     /locus_tag="IWM52_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAP9974201.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21593..21964)
                     /locus_tag="IWM52_001955"
     CDS             complement(21593..21964)
                     /locus_tag="IWM52_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP9974202.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21983..22870)
                     /gene="xerD"
                     /locus_tag="IWM52_001956"
     CDS             complement(21983..22870)
                     /gene="xerD"
                     /locus_tag="IWM52_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAP9974203.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6443 a         4913 c         3899 g         7732 t
ORIGIN      
        1 cttgtcacag tcccttattc tgaataaacg gtgtggaaga gacagcctat cagtagtaat
       61 aagacacatc tctttcacag cctcattttt gcgtaaaaat cgatttattc gaaaaattcc
      121 ccgtatacat cttctacacg ttgatctgga ttgatcacga tatacaagtt acctgttttt
      181 tcgctgattt ttgttgattg atattgattg tctaatcctt ctgttccttt atctagatat
      241 gggaagtaca caagatcagg tgtgccattt cggtaaagga tttccattcg ctcgatatct
      301 tcgtatttca atgcgcgatt aaacatgccc atttccagtc cgcctaggtt gatatcatgt
      361 gtcgcaactg cgtccccttc atggaagatt tcgattttaa atccagcaca cggatggatt
      421 tcaacaaatt cactgccatt gattcgtcca aaacttgttg tgatttgttt gatccaaaga
      481 tcgccgatat gacggcggtt gatcgtccag gtttctccat ttctaaaata aaattttagc
      541 gctgtcattt ctttactcat taaaaagact cctttatgat gtaatttatt cttgtactac
      601 ctcagtttag cacaaattta tgtaagtggt cacatcttat gttttttctt ctaatgacgt
      661 atctgcccac tttaagtagc caacaaagct gagcagatac agtctagtgt tttttaaatc
      721 aatttttctt tcgataaaaa atccaaaacc tgttttttat cattttttat tctttgttct
      781 acgacggctt tttcagctaa ttgcaaagct tcactaaccc acttaccagg tttttttcca
      841 gtttcttcta gcaagatttt accatctact gctaattcgc ttaatgactt gataggtaaa
      901 cgatcatagg attctcctac ggcatccatc tgaggtgttt cgtcaaaata gatcatcaat
      961 tcttctactt gcagtgcatg ttttttgcct aagaagtaca attcgaatgg ttccaaagct
     1021 ccagtttctc gttgtcgtag tccttggaca agtgcttgaa cgtctttaat catttggtta
     1081 gaacacttcc acgatttcaa aaaggaacga atctcatttt cttttagatc cagttggctg
     1141 atcaataatg cccatgcttg gctctcttct ttcaatggga cggatggtaa ttcagcaaaa
     1201 cgtaaaagtg cttcgccttc ttctttcaaa ccgggacagc aaatgtagca ctctgtttca
     1261 ataaatgcat ggattcccgc ggcccgttca tttcccaaga gcatcttgat gaattccaca
     1321 tttatacgtt ctacagatat tttacctaat agctcgtggt tttctttgat ggcgtagaag
     1381 gtttcttcct ctaatttgaa ccctagttga ctgacaaaac gcaacccgcg catcattcgt
     1441 aaggcatctt catggaaccg ctcatgagga ttgcctacag ctcgcaagat ctgttcttct
     1501 agatcttgca gaccatcgaa caaatcgatg atttctccat cttctttcaa agcaaacgca
     1561 ttgatcgtaa aatctcttct tttcaaatct tctgccaacg aacgaacgaa ttcgacatga
     1621 tctggtctac ggtagtcctg ataagttgat tccgtccgaa atgtggtgat ctcatattgt
     1681 tcatcatttt ctaataccaa cacagttcca tgttcgatcc caatatctat cgtttttgga
     1741 aaaagctgtt tgatttcttc tggaaatgcg cttgttgcga tatccacatc atggattggc
     1801 tggttcagca agatatcacg aacacttcca cctacaaaat aagcttcata tcctgcattc
     1861 tctaattttt tcagaactgg aattgccttt tggtattcca gaggtaattg ttttaatttc
     1921 atttttccaa cctttcctgc gtgtttcttg tcctgagata aagactcagt tgcatggtga
     1981 ctgtttgaca agtccgtttc agagtcttca aggccaggac cacttcatct tgtttactcg
     2041 tccttacgct caaagcgatt atggtctctt tgattgaatt tttccatgtt tttttcaaaa
     2101 acttcagtta agtcgatatt caatgaatta gccatgatca tcgtaacgaa taaaacatca
     2161 ccaagttctt ctgctaccga gttaactggt tcagaagcct ttttgctttt ttctccataa
     2221 gtgtggttga cttctcttgc caattcacct acttcttcag ttaatcgagc catctgagct
     2281 aatggagaga aataccccac cttgaattgc tggatatatt catccacttc ctgctgcata
     2341 ctgtataaag aacgcttttt atccatcttt ttccctccaa aactatctta ccaaaacttt
     2401 aacgcaaatc ctagataaga gcaattgaga aaatcgaatt tccatgctac atttaggaag
     2461 gaatttctaa atggaaattc tgtgaaaata cataattgaa agctggaatt catatggtca
     2521 aacgaaaaac agtaaaagaa gttcttttta tcattatagg aacaagtatt tatgcatttg
     2581 gtcttgttta cttgaacatc gctaatcatt tagcagaagg gggagtctca gggatcacct
     2641 tgatcttaag agctttattt ggtattgatc cagcttatac gactttatta atcaacatcc
     2701 cattgatttt actaggagga aagatcctcg gtaaacgttc cttggcctat actgttctag
     2761 gtacactttc tttgtcattc tttttatgga tctggcaaag ggttccacta gaaatcaatt
     2821 tacagcatga tctactgatc gtttctctac ttgcgggttt agttgcaggt gtaggaagcg
     2881 ggatcgtcta ccgcatgggt ggaacaacag gcggcagtga tattatcgct cggattttag
     2941 aacaaaatta cggcatcact atggggcgtt ccctattggc atttgatatc atcgtcttac
     3001 ttgcttcatt aacctatatc gacttgaaac gtatgatgta tacgttgatt gtttcgtatg
     3061 ttttcagtcg tgtgattgat tcgatcttag atggcggata ttctgcaaaa ggtctacttg
     3121 tcgtctccaa caaaagcgaa gagattgcac cactactgat gaccggtctg caaagaggtg
     3181 tgactttcct ttctggagaa ggcgcattct ctggtaattc aaaaaaaatc atctatatgg
     3241 tcgttagctc tagtgaatta actgaagcta agaggatcgt ccacgagatt gacgaaaaag
     3301 ctttcctttc aatcatcaat gtgcatgaag tggaagggca aggctttact tatttgaagc
     3361 cacagacaag attactgaaa aaactataag aaaaaagaca aagataggct atgaggaaag
     3421 attccacatc cagctgacct ttgtcttttt tagtactata ctatttttaa ctaattgtta
     3481 tgttcactta cccggttact tttcttttgt gtctgcaaaa ggatctttcg gttaacttca
     3541 tcagataatt tcaaaaaagc atccctatct ccatcttcta gtgcttgatc gatttgagca
     3601 taaagcattt ttagttttgc ctcttcttct ttttcttgaa aatatcgttc aattaattca
     3661 gtatcttctt cactgactga agaatcccaa gaagaaaatg gattgtcttc taagatagat
     3721 agatattctc ttgtctgcca agcattttcg aatatacatt ccacatataa aggctttttc
     3781 caattcaaac ggatttcatg aaaaatctgg tctgtgtccg taaattcttt tccctgcaaa
     3841 aacaatttca tgggttcccc ttcaacagat agatcactga tctgcagtcc tcgatccgca
     3901 aaattcgccc cttcaacaaa gtggacattg cttaaaatcg cttcatgatt ggcaagataa
     3961 ttcaagatcc aaaaaacttc tcttcggcta aaagatacat gattgaccat ccacgttaaa
     4021 aaattcttct tttcccgtac atcaattagc atttttctca cctctctgtt aggtcatcta
     4081 ataatgacag tgcctccata tctcctggtt caagtgttaa ataataagtc aataattctt
     4141 gcgctctttt cagctgccct tcttctctta agaaaaaggc atactctttc aagaaatctg
     4201 gttcatgtga tagttcatga taagcctgtt catagtgtac agctgcaacg gtaaagtctt
     4261 ctaattcatt ataagcatgg gctaggttcc attctgcata aggattagca gtttcttcca
     4321 tctgattgat cacttgtatc acatcttcat atctttcatc atccaagtaa agattactta
     4381 aagtaagtaa tgtttcatct tgtttgtctc ctacttctaa agctttcagt aaaagttctt
     4441 ctgctcgctg tctatcatgc agacggaaag cattttccga ggccaggtga taaagttcga
     4501 cttggtaagg attttctttg attccttctt ctaacactgt ttgtgcttct tcaatcattt
     4561 cttcttcttg aagagcttgt cctagatata gatataacga ttgataatgc ggattaactt
     4621 ctcgaagttc ttccagatag ttgattgcct gctgattttc tttcaattga agattcgtaa
     4681 aagctagctg gaacagtcga tcatctgtcc tttcttcttt cagtgcttct tctaaaggta
     4741 cgatagctgc ttcgaattct ccttgcatac ttaaggattg tcctaaacgt tcctgcatcg
     4801 agattcctga aatttcattt atcccgcttg ccaacaatgt ttcataaacc actgctgcct
     4861 ctttgaaacg atcaactgaa aaatacaatt cagctagcgc aaactggatc aatggttcat
     4921 caggaagcaa cccagctgct tctttcaatt ttgcttcact cacttcaggg attccgataa
     4981 cttggtacaa atcagctatc gccaataagc tttgcggata gtaatcactt tgcttatcca
     5041 ctttttccag gtattcaaaa gctaagtcga tctcattatc ttcgattgca atttctgcta
     5101 acgggatgtt cagtccgtca ttttctgggt attggttgat cagttgttca aaaatctgtt
     5161 ttgcttcgat caaaaagcca atagccagca attcttcgcc tagttcagct aatatatctg
     5221 ctgggtcttc ttttaatgct ttttctaaaa agatttgtgc ttgtgacaag tcttcatctg
     5281 ataaagcttg caacattttt tcgctatttg tattcatttc aattccctct tatctcactg
     5341 acgatttgtt gaaataccgt cattgttctc aataattctt tttcgtttgg atgttggtat
     5401 ccgtctatta tacctatttt ttctaaaaca agtatttttt tgcttttttc tgccaaaaaa
     5461 agaacttttt ccaaatactc catctttgaa atctgttcca gccagtggat tggatagcca
     5521 agcaagtcca gccacacaaa aaagtttttt agttggaaat caaaaggata tgcttggaga
     5581 ttccacacga aatgaaacat cattcctaaa aattttttca tactagcaga aagtagatgg
     5641 ccattttcgg tcagataaaa agcttgttca aacagtttac cataatttga caggttttct
     5701 gcctctaatt gataaaaata gatcatttct tcgatcatcc cggcaaacgg ccgagtcgtt
     5761 aattgctttt tatttggata gtttttatac agattttgca aaaaacgatg atcacaaatc
     5821 attccgcaac agataaaagt caaaaagtca atctgctttc cgtccacctg ccgctcgctg
     5881 attgtttgat cataaacgat gcgttctggc agattgatgg tttgcagcac tacacgattt
     5941 ggtagctgca aaatcgtccg ctgcggtgtt aatgctcggc ttagagaacg gatcgataca
     6001 gggatcatcc atagtgatga atcaagggtt gtatgttttt gaaaaaaacc tgccagatcc
     6061 atgatccctt catttccaaa tccgatgata aacatggatt gattttcggg atagcgtttg
     6121 acgaaatcaa gcaggttctt taattctgtt aaatgattgc attgagtgtt ggcacaaata
     6181 taccagtcat tgtttgcttg atttgcaaat aacgggttga tcttatctgc aaacaaatca
     6241 taatagcgct gattcgttaa aaataagatt ggctgatcaa tggaaagttc tcttagctga
     6301 ctgactagcg tttctccgta aaacactttc gtcaacgcat cttctacttg ataatcaagt
     6361 tccatacgca tccctcctct tcttattttt attttaccat tagtaagcaa attctagaaa
     6421 agataagagc aaagctcacc tcataacttg gaaataaata aaaattaaat aaaatacatg
     6481 tgttttctat gtatgacata cggattcgga atctctgtct cttatttatt ccttttctgc
     6541 taggaaataa aaagaaaagc acttgtagcc ctagagccac aagcgctttt tcaactgtct
     6601 tatttaacag cgtcttttaa tgctttacct ggtttgaatg caggtacttt gctagctgga
     6661 atttcgattt cttcaccagt ttgtgggtta cgacctttgc gagctgcgcg ttcgcgaact
     6721 tcaaagttac caaaaccgat taattgaact ttttcgccgt tagctaaagc atcttgaata
     6781 gttgaaaata cagcatctac tgctgcagtt gcatcttttt tagttaaatc agtagctgaa
     6841 gcaacttttt cgattaattc tgctttgttt gccatggata gattcacctc ctccacaaga
     6901 gatatgatag aaatatcatt tagttaaaac tttctgagtg gtccagaagt ttaaaaagaa
     6961 atattgatta acttcaatat tctgttatca cgatatcata tgaaccgctt aatagcaagg
     7021 tttttaccaa ttttcacatg ctttttttag tcatagaaac gtaacaaacg aaactgtctt
     7081 tttcttttga aaaaaaaacg tcgatatggt aaaatgccta ttttctccta cgagggataa
     7141 ttcggatgga cgtcccttca aaagtaaatg cttttctgat ttgattttct aagaaacgtt
     7201 cataagaaaa atgcatcaat tcctcttcat tgacaaaaac gacaaatgta ggtggtttga
     7261 cagcgacttg tgtagcataa aaaatcttca ggcgttttcc tttatctgtt ggtgttggat
     7321 tgattgctac agcatccata atgatatcat tcaacacagc cgaagagata cgaagattct
     7381 ggttcatact tacttgttcg atcatttcag gcagacgttc caaccgttgt ttagtcactg
     7441 cagaaacaaa aacgattgga gcgtaatcaa gataccggaa ttcatcacga atctcttgtt
     7501 cgaaatcacg cattgtattg gtttctttct ttacaagatc ccatttattc acgacgataa
     7561 taattccacg accagcttca tgggcgtaac ccgctactcg cttgtcttgt tcccgaatcc
     7621 cttcttcagc attcagtacc atcaaaacga tatcagaacg gtcaatggca cgcattgcac
     7681 gcatcacgct gtatttttca gtattttcgt atacttttcc gcgtttacgc ataccagctg
     7741 tatctatcat taagaatttc tgaccacttt cagaaacgaa gtgggtgtcg attgcgtctc
     7801 gggtcgttcc ttcaatatcc gaaacaatta cacgttcttc tcctaaaatc gcattgatca
     7861 aagaggattt accaacattt gggcgaccaa tcaaactaaa cttaatcgtg tcgtcgtcct
     7921 cttcttcagt ctcttcagag aaatacttca ctgcttcatc taaaatatca ccaatcccta
     7981 gtccatggct tcctgaaaca ggataaggat cgcctagccc taaggaataa aattcataga
     8041 tatcgtttct catttctgga ttatctactt tattgactgc taaaatcact ggtttattac
     8101 ttctgtataa aattttcgca accatctcat ctgcgtctgt cacgccttcg cgtccgctga
     8161 ctacacaaat gattacatct gcttcttcaa tcgcgatttc tgcttgatgc ttgatctgat
     8221 ccataaaagg ttcatcgcca agatcaattc cgcctgtgtc gatcacgctg aattctcgac
     8281 ctaaccattc accttttgca taaatacgat cacgagttac tccaggagta tcttcgacaa
     8341 tcgagatacg ctcaccagcg atgcggttaa aaatcgtaga cttaccgacg ttcgggcggc
     8401 caacgattgc aattgttgga tttgccatat tatttcctcc tcacaagttt ccaatctcac
     8461 ttagtttaac gaatgcattg ccaactttca agcatagatt gtcaattaaa cgaagtattt
     8521 ttattcctta ttgaaatact aatcgctttt caaaagaaaa actgagacaa aagcagctgc
     8581 tttcatctca gtcacagata caaaatcaat tatttttcag aatcatcggt tgctttttct
     8641 tctacttctt ctttcaacgc atcacctaaa atgtctccca tagtaaatcc agtattttct
     8701 tctggtaatt cataagactc aacatttttt ggttcttctt ctgaagctgg tttttcttct
     8761 aaagctttga tactcaatgc aatacgatgt tcttctggat ggatctctaa tactttcact
     8821 ttcacttcgt caccttcatg aagcacttca tgtggtgtcg caatatgttt atgtgaaatt
     8881 tgagagatat ggactagccc ttctactcca gggaacactt caacaaaggc accgaagctt
     8941 gtcaaacgtt taactgttcc gtctaatact gtgccaactg gagcttttgt atcgatttct
     9001 gaccaaggtc caggctgtgt gtctttgatt gacaaggaaa cacgttcttg ttcaggattg
     9061 atagacaaca ctttgacatt tacatcatca ttgacagcca atacatcact tggttttgct
     9121 acatgactat gtgaaatctc tgagacatgg actagaccgt caattccgcc taggtcgaca
     9181 aatgcgccaa aatcagtaag acgtgcaaca cgtccactga gaacatctcc ctcatggata
     9241 gttgaaagaa tttctttttt cttttcttct ctttcgtttt ccactaccgc tttatgcgat
     9301 aagatcaaac gattttctga tggttcgatt tcgatgattt taaagctcaa tgttttgcct
     9361 ttatagtctg aaaagtctgc aacaaaatga tcttccacca ttgatgcagg gacaaaacca
     9421 cgtacaccga cgtcaacgac taaaccgcct ttgacaacat ttgtaacagg tgcttcgatg
     9481 accttgccct cttggaaatc tctttcgata tcttcccaaa cttttttagc atctaaacgg
     9541 cgttttgaaa gtaggtagct gccgttttct ttatctttgc cgatcgtact gataacgact
     9601 aaatctaata catcaccgac ttttactact tcgctgatat cttctaccgg caaagttgat
     9661 aattcttttg ccggaacgac tccttcaact ccagcccctt cgattccgac aatcacttgt
     9721 ttgtcttcaa tgactaaaac ttcgccctta acaacatcgc caacttttac ttcttgtacg
     9781 cttttcattg cgtcttccat tgactggttg ttctcttgat tttgatcaaa ttctgtcata
     9841 acacttgtcc tcctaaccaa cattctgccg taagtacggt actgttttta gtttaattga
     9901 ttattcaaaa aaaataaagc gatttcgttc gttttccttg aaaaaacaat tgtttttcat
     9961 tttttttctt aatttaaaaa agaagataac cttttttcaa aacaacagct tttattgctg
    10021 caacgacttc tggaatcgat ttgccagtcg tatcgatacg aacagcatcc tctgcttgct
    10081 tcagcggaga gacgtccctt gttgaatcat aatggtctct ttcctgtatc tctttcgtca
    10141 gtgtttcaag atcagtcgga attccttttt cttggttctc tttgaaacgt ctttctgcac
    10201 gctcacttac gcttgctact aaaaagattt tcacttctgc attcggtaat acagctgtcc
    10261 cgatgtctct tccgtccatg acaacgccac cgcgttcccc tatttttcgt tggacagcaa
    10321 ctaattcttc tctgactttt ccatgtgcag agacttcaga aacagctttt gtgacatctg
    10381 gttgacggat ttctaaagtc acatcttttg atgctatgaa tacatgctgt ccatcttctt
    10441 gttgcgaaaa agagatagga tactttttga tcagcgaaac tagcccttct tcatctgaaa
    10501 aagatacatt gttcgtaata gctaaataag ttactgcgcg atacatggca ccggtatctg
    10561 tataaatata attcaattct tttgctaata tcttggcaac ggtacttttc ccagatgaag
    10621 caggcccgtc gattgcaata ctgatttgtg tcatgatata actccttttt ctcagaaaaa
    10681 aagccgcaaa gcgcggctat tattatttaa ttcttaattc ttgaccaggg taaagcataa
    10741 agttgtttgg atctaagcca ttcaattcaa acaattgatc agtggtgatc cctgcacgct
    10801 cagccacttg ttttggacct tcgcctgctt gaacagtcgt tgttgaaccg gctgcagctg
    10861 atgaagaagg tgttgtttct gctgcattgc tgcttgaatc ccctgcttga taagttgcct
    10921 cacttgagga agtcggtgtt gtctctgctg caatactgct ttctgacgtt acacctgtat
    10981 cttgagacac agtagattct tccgcgattg tactagattc tactgttgaa gtctcagttg
    11041 actcctcttt tgttgtactt gtagaacttt ctactactga tgtcgaagtt tcagaagaag
    11101 ttgctgctgt tgcattgttg cttgatccat ttctgatcca gaaataagca cctgctggaa
    11161 tagcgataca taagaccagt aaaatcaaaa atagcgaaag aaataatgta tttcctttct
    11221 tttgttgtct ctgagaactt cttgatgaaa cttcatcatc tgtatcgtag attggttgtt
    11281 cccatggttc ttgtgtttcg ttattttctg tagattgatg tctatctttt ctgctcactg
    11341 ttttacctcc taaactctat cgtgtgtatt ataccatagg aaaaagtgtc ttgtcctaaa
    11401 aaatcaatgt tccatgtttt ttttaaataa atttagtaaa atcgtttccc aatgaagttg
    11461 ttcagtctta gaaaaaagct tcctctttgg cagaagttct gcttttgctc catcaatatc
    11521 acaacaattc tgcggctttt cggagagttt ttcgccaaaa taagctaaga tgaattttct
    11581 gcggcagttt tcttcattaa tatagcgaag catctgctgc aggcggaatt ctttttcttt
    11641 ttcctgacgt ttgagccgtt ccacccaatt ttctggtttt tcagactgct ggatcagctc
    11701 catccatttt ttttgcagct catcaaaggg agcttgcttt gtggcatatt ccaaataaag
    11761 ctcaaaactt tgtcgctgtt ctctagaaag ttgattaaag aaataatgga tacgttcatc
    11821 cccactttga taaaaaagaa ttgctgcact ttcttcctga tctctgcctg ctcgaccgat
    11881 ttcttgtaca tagttttcta gactgtctgg caaatcataa tggaccacat aacggatatc
    11941 agatttatcg atccccatac caaatgcatt cgtagcaatg agaaactgca gctgattttt
    12001 tacaaactgc tgttgaagtt gtcttctttg agctgcatca agaccaccgt gatagtatcc
    12061 aactgtaaaa cgttccctga ataaatgata caatcgttcg acttcttttt ttgttgcaca
    12121 ataaatgatt gccgctcctt gtgctctttc catgaagtgt tcaagttctt gttctttttg
    12181 ctgcgttttc cgaacaaaaa gcgagatatt ttgccgattg actgattgtc tgatctcttt
    12241 tggtggtcga tcaaatagta catgacgtat atctcttgct actaaatctg ttgctgtcgc
    12301 cgttagtgcc agtgtgaccg ggtttcctaa aagctttcgg atttttccta actgttgata
    12361 ttctggacgg aaatcaactc cccattgaga cacgcaatga gcctcatcta ctacatataa
    12421 cgcaatattt tgttttttca gatgctctaa aagagaagga ttagtcaaca tttcgggact
    12481 caaaaacaaa aatttatatt gcgatagatg acggaggacg tatcttcttt cagattgcga
    12541 aagtaaacta ttgaaagcag ctactcgttt ctcccctcgt ttttgtaatt gcgtcacttg
    12601 atcttccatt agagaaagca gcggtgacac gatgatgacc agaccttcca ttaaataccc
    12661 cgtcaattga tagcaaaggc tttttcccgt tcctgtaggc aaaatagcga gagtatcttc
    12721 tccatcaagc aacgcctgaa taatttcttc ttggccggga cgaaatgaat caaatccaaa
    12781 atattttttt aattcattta ttaacattct gtatcgcttc ctttctcaat agatagattt
    12841 gatacaaacg aaattctcca taatctaatt gtccatctac attccattcg ctaaagcgcc
    12901 actctcgcac ggaatcctta tttggttcca aacgttcgat tgtttctgca gagagaatcc
    12961 actcgtatgg aaaatcatca aaaaacaagg cccactcaat aagatgatct gttactgttc
    13021 ctctttttaa atgacggatt gccatgatct ggtcaattgt ctgaccattc ataaacatct
    13081 gcttggtgat catcatgctt tgattgaaat tttgctgtaa taatgaatcg atcaaagcgt
    13141 gccaaagcga atctggccgt ttttcgattt gtgctaacaa aaggtggata catctgcttt
    13201 gttgcagata aacctctgac tcatcattgt tttttgccag ctgataagga agcagccctg
    13261 ttcgctcatt tccagataat tggttagcta aaaaatctgc tgcttcttct ggtatctccg
    13321 aaaacagctg caccaaactc tcatacgcag tatcaatcaa aatttcacgt ggcaattggc
    13381 tttcactaag ccattttttt agttggaaag tataaaaagg acttgtctct gctggaagat
    13441 agtcttgaat acccgttgcc agattagaaa ttacttgaac agcaaattta atgagccgcc
    13501 aactagtttc ccctgttctg ccatagcggt caaaacgcaa accagtatgt tctatttgat
    13561 gttcggacaa catatcagcc cctgcaggcg ttagctttgc ttcattttca tttatttcga
    13621 tccaaccttg ctgcgcaatt ttttgcaaaa tttgataaaa cttatcctgc ttcaattctg
    13681 gaaaactgtt atggataaac aaaagttcgt gggtaaatcc ataaataagt actgaagtcg
    13741 ttcgttttcc cacaaggagt tggtaaagag aactaatccg cagcttgtgt cctgatgaaa
    13801 atagcgataa aataaaaaca atcatattat tctccttaag ttaggatggt tagaaatgtc
    13861 actactatgt aaacttgtac ctgaacgctg tattgcatgc ggtctttgtc aaatcgaagc
    13921 gccagatatt ttcgattatg atgaagaagg aatcgtttta tttgcccaag aaccagaggc
    13981 tcatcaacag ttcgtgatgc cggaagaaga aacatatgta aaaaaagcgt atcaaaaatg
    14041 tccagttcga gcgattttat tagaaaataa aatataaata taaacgaaac tttaaaaacg
    14101 tacactaagt tctctaaact tgaaaataag ccgggatctg tttctcttgt tccgtgaaat
    14161 cccgacttat tttgaaagat tcatttgcta tctatagtta attgactagt tttttcacgg
    14221 accctatagg acccatttat attatttcaa ctttttcagc cagttttcca atgtattgac
    14281 gatcacagtt tgctggtctg cgttagaaat ttctgcctct ccatctcctt tttgctcacc
    14341 atagctgcca aaacctccat gatttccgcc attgattgat acatactctg ttctttccgg
    14401 taaatactgc ttagcttctt gataagcttc ctgattcaat actttatctt ttgtcgccgt
    14461 gatcgataaa acaggaacag ctgattctgt caatgttcct tttttgtcgg gataacttgc
    14521 catgaagaat acccctttta aatgtttgtc tgaatgattc ttattgacat aacgacttgc
    14581 cattacgcca cctaatgaat ggccaccgat cacataatcg gaatttggaa aatcttcttt
    14641 gaccttatcc gctttgtttc cgcccatcac tgctagatca agtggcattt tgacaatagc
    14701 gactgaaaag ccgttttttg ctaaatcttg cgcccaaata ctataacttt ctggctctac
    14761 gagagcacca ggataaaaaa taagtaatgg agactttcca tgattctttt tctctccttc
    14821 aaaaaataag taatcttctt tgttttttcc ttcttttacc aacacttgcg cttctgacga
    14881 tggttcgtac gtattttgct gtatatagta ataaccggct ccaccgagta atcctaaaat
    14941 gatgactaag ccaattagta ttcgtctaaa tattttcact ttctcccacc ttttctatct
    15001 gagtgacccc tttttcttaa tcaagtatga ttatacctta aaaaaataag agagaaatgc
    15061 aaactttcca gcatcaaata aaaaaagacg gaatatccat ttgaatatcc gccctttttg
    15121 tatgtttatt tttatttagc acataaacga tacacagctt ctgcataaat ttccattgct
    15181 ttatacatac tacttaattc ccatcgttca ttcgcctggt gcataaagtc aggagtatca
    15241 gggaacatcg caccgaatgc cacacactgg ttcatcgtac gagcaaaagt agcgccgcct
    15301 gatacaaacg gttcagtcat gtctccagta atatcgcgat atgtccccaa tagatttttc
    15361 actaactcac tgtctagtgg cacgtataat gaatcaagga agtcaaattc ttcataagtc
    15421 aacccatatt tttctgctgt tttagttaat ttagctacta aatcatcttt cttgaatgta
    15481 acagggattc tcatatctac accgattttt gtttgttctg gtgtgatttc caaacttgca
    15541 aagttcatcg ttagctcgcc ggattgttca tcttctgttt tgcccacgac agcttcacca
    15601 gttgcatttt cttgaactaa ttttcctaag aaattgattg ggccaaaatc aaatacttct
    15661 gaaagagcaa ttgccagacg agaaatagca ttcactcctt gtgctgcatc tttcgcgtgg
    15721 atacttttac caagaacgac gatgccttca ccttgttctt cgaaatcgaa ttcgtgtttt
    15781 ttcaatgctt cttttacttc agaaagcttt tctccagagt aaggagctgc atcaggaaca
    15841 acattcaacg ctcctccagc ttttacagaa aactcgttcg ttcctggtcc tgtcaagtaa
    15901 gcttgcaata agcctttttc tgcataaatt aaaggaaatt cagcatctgg cgcaaatcct
    15961 tgcgtaattc cttcttcttt ttcgttatat ttttctaaac aacgccataa attttcttca
    16021 tctgtaccaa agataaaacg aatacgtgtc ttgaattcca cgccagcatc aatcaaagct
    16081 ttgacggcat acattgccgc aatagaagga cctttgtcat cttgggatcc gcgaccaact
    16141 aaccagccgt cttttatagt tggatcgaat ggttttgttt cccaattatt ttcgtcccct
    16201 gctggaacaa cgtccatatg acacaagata ccgaataatt catctccaga gccaatctct
    16261 gaataaccgt aatagccttc tggatcttta aatgttctaa atcctagctt ttcactgatt
    16321 tccaatactt tatccaaagc ttcaatcact tttttaccga agggatgtcc ttgtcctgaa
    16381 tctgcttcat ctagtacgga aggaattcgg atcaattcgc tcaatgattc taatgcttgt
    16441 tcatgatgtt gttctgtcac aaattttttc atgatattat cctcctaata tgttaaaaag
    16501 ggtcatgtca aattcctccg acacaacctc ttcttctcaa ataatgacgt tttaagcctg
    16561 ctccatcagc aagcagaatg ttcttaacaa atgatgaaag aaaaaacact cactgaatgg
    16621 agctattgac ttgtccacgt gcttatcgat agccgtttat cggtttcttc attcttatga
    16681 tagaccttgt acctgtattt gtctaggttt agaacattgc tgctacacct aggatcacac
    16741 agatggctac aaataatatc gcaattaatt taccggtaaa tttccaccac actgaaacat
    16801 cgaaacgagc aattgctaca gcacccatta cgatagcaga tgttggtgta attaagttta
    16861 ctaaaccaga tgcagattga taagcagtga ttactaaatc cttaccgacg ccggcaaatt
    16921 cacccatagg tcccatgatt cccattgttg cagcagctaa gccggaagtt gaaggaatca
    16981 agaatgacat agggatatag aaaataaatg taataatgat gaatacactt gaagataatg
    17041 aacttaatcc ttgttcgccc caatgtaaga tggtgtctgt gattaatcca ttgttcataa
    17101 ccacttggat accacgagca acagctacta cgattgctac accgatcaaa tcagaagcac
    17161 cagctaaaaa ttcagagaca aattcagact ctttcatgcc ataagtgatt gctactacga
    17221 tggacattac taagaataac atagtaattt ctgggaagta ccagtcacct aatggaagca
    17281 tgcttttacc tagaaccgta cctagtaccg gaaggtttgt taaccagttt gtcaatgatt
    17341 caaaaatcgt gaaattcgca ttcaaggttg tccaaggaat caaactgatg atcatgatac
    17401 caaaagtaat aaagaacaat acattaacat gtttttgttt tttcgtgatt acttcttgac
    17461 gaccgattgc ttcaatatcg aaatgcttca tatcttcttt acgttggttg tatactaatg
    17521 attttgttgg atctttttcg atctttgatg cataacgata cacatataca atagaaatag
    17581 ctaacaaaat aacgaacatc aagaatcgaa gaccaatacc ttcacccatc gaaatcccaa
    17641 cagcttgtga agctacgcct gttgcaaaag gatttaccgt tgaagctaga cagcctactt
    17701 gagaccctac taatacgatt gctacagcta ctactgtatc aaagcctaca gccatcatga
    17761 ctgggatcag taaagcatag aatggaatcg tttcttctgc cattccgtaa gtagagccgc
    17821 ctaatgcaaa taacagcatc aatactggaa taagcatttt ttcttttcct ttgaatttct
    17881 tcacaactga agcaatcccg gcatctagtg cccctgtttt gttgataata cctaaaaatc
    17941 caccaacaac caaaatgaag aaagagattg ggattgcagc aggtgtttct ttagttagct
    18001 cccccgctgg tacacctagc atcccattga ttggcgccat gaaaatatcc cacagccctt
    18061 gaggattttg ttgtactcgt tcatatgatc cagcgacgat gttgcctgca tcgtctacac
    18121 tatagtgccc agctggtata aaccaagtaa gtacagcgat tactgcaata ataataaata
    18181 agaccgtata cgctgaaggc atttgaaatt ttttcttttt tgtttgttcc atcttcttct
    18241 tcctttcttt tccgttagag acaacaattt tggctctaat gttattttat caaaaagagt
    18301 atcagaacaa aaataataac acgcattttg taaaactttt gttcaaaaag ttcgtttttc
    18361 cctattccct cctctttttt cagtttttta aagctttctt ctttttcatt gtagcgtttc
    18421 ctttttttta taaacaaaca ttttcgtctt ttaaaaggag aagaatttca catttgcaaa
    18481 gaattgattg atttttatgc ataaatcaaa aaaatactca aaacaaacaa ataaaacgtt
    18541 tacattattg catgtcttgt atttttttcc aaaaaaaaag accaagaaat ttcttgatct
    18601 ttttgatatt tatttgactg ctgtatgacg ttgttcgaac tgatgttgtt ttcgacttaa
    18661 ccatggaaga agttgcgcat gcagcactaa aaagacagca ctcacgatga atcctttgat
    18721 caagttgaaa ggaacgatcc caatcagtac atagtttgct aaagacatgc ccagcatctg
    18781 tgttgcgttc agccctaaaa acattaagta aagaggcgta ataacgaaat aatttgcgat
    18841 actcataaaa atagttagag cgatggttcc tgtaatgaca cctaaaaatt tgtttcttcc
    18901 tttatgctgt ttctttacac taaagaaata ataaatcggg aatgtaaaga tcgttgttga
    18961 taagaaacta gcagtgtctc ctaccaaatt atccggagaa aaacctgtag tcagcagatg
    19021 taatactgaa cgcaagaacg ctgttgcaac tccggccgct ggaccgaata agaacatgct
    19081 gatcaatacc gagatatcac taaaatcgat cttcaaaaaa ctaaaagccg gcatgatcgg
    19141 aaaagcaaaa tactgtaaaa ctaatgcgat cgcagcaaac atcgctactg caaccatttt
    19201 ctgtacacgg gtgttcttca tttgaaatcc tcctgttagg actcctcttc agcgaacttt
    19261 ttgacgtttc tctccaaatg aaaaaactct attttccagg gaaaatagag ttagagttat
    19321 ttgatcaagc gtcgtcaaat aagctctatc ttctttatcc agactatact gtcggtatcg
    19381 gaatttcacc gattcagcta tttttttgca aaataggtcg tggactataa ccaccggtcg
    19441 ggaatttcac cctgcccctg aagacgaacc ttatataatt tttgtttaca tattcattat
    19501 acatatctat tcaaaaaacg caacatctaa caattataaa aagtaagtgt tcattttgag
    19561 tggttcagca aactgctgac ttcttttttg ttcagttcac ggtaatcccc aggacgtagg
    19621 ccttgtaaag tcagatctcc gtatttttca cgttttagtt tttgcacagg aagaccaact
    19681 gcttgaagca tatttttcac ttgatgatta cgtccttcgt ggatcgtcaa ttccacgata
    19741 ctgtggtttg tcttaggatc cactgataaa atttggaatt tagctggaga tgtttttcgt
    19801 ccttcgatct ttattccttt tgtcaaaggt gcaagcatcg attttgtagc gatccctttt
    19861 acctttgcaa cgtaaacttt atccacttca tgttttggat gtgtcaggcg ctgagcaaaa
    19921 tctccatcat ttgtcagtag caagatcccc gaagtatcgt aatccagtcg acctacagga
    19981 tagatacgtt cttttaccat cggcaaaaga tccgtaacaa cttttcttcc tttgtcatct
    20041 gcaacagcag aaataactcc tttaggtttg tacaataaat agtaaccgta ttcttcttga
    20101 taaattggga cttcatccac ttccacagtg tcatgtttcc caacttttat ccctaattcc
    20161 cttacaactt gtccgtttac ttttacacgt cctgcaagga tgaattcttc tgcttttcga
    20221 cgagaagtga tgccagcgtg ggcaattact ttctgcaatc tctccattac aattattctc
    20281 ctttattcat tccatcaaag aatagatctg gtgacacgtc ttcttcaagt gaaatttcca
    20341 tttcttgtgt atcaggcagt tcttctaatg atttcaaacc aaaataatct aagaagtagc
    20401 cagttgttcc atataatatc ggccgacctg gtccttctac tctacctttt tcttcaatca
    20461 gtttatgggc agctagacgc tgtaccgctc ctgaaacctg aactcctcga atctgctcga
    20521 tttcagctct tgtgattggc tgtttataag cgatgattgc taaggtttcg acggcggctt
    20581 gagataatgt gtttgatgtc ccttgcgcat atcgcttaag taagggagcc agctcttttt
    20641 tagtcgttaa tacataatgg ctttccgttt caaaaatatg caaagctgag tgcgagtttt
    20701 gagtgtaaga ttcttttaat tctttcacta aacttgtgac ttctgcttcg ctgagattta
    20761 acagtccgct taattcttca ttactgatcc cttcctcgcc aacaataaaa agaagggctt
    20821 caagttcact taatttcgtc atgttcggta tctcctactg ctggataaag cataattgat
    20881 tcatagtttt ctttttgttg cgcaatgatc tgcccttttt tcatcaattc tagcaaagcc
    20941 ataaaagtag tgacaacttc acttttagaa ggtgactcaa aaaaagaatc gaaagtacag
    21001 ccatgcgcat ttctttttat acgctcttcg ataaatacca ttcgttcttc gatcgttgtg
    21061 tcatcgggcg tgatcgtttt ttccactttt tgagaaaatt tcttacgttc taagattttc
    21121 tgcatggcac ggatcaaatc gatttttttg acttgtcctc gcttcaacgg cttttcagca
    21181 tccgctaacc catcgatgtt cataggttct tttgtgtaat aaagacttcg ttcctctgct
    21241 ttttctgtca gtctttcagc tgcatattta tatttacggt attctaaaag ctgagtgacc
    21301 aattcttctc gaggatcttc ttcccataat tcttcatcgt ctgtgccgaa ttcttggaca
    21361 ggaagcaatg tcttgctttt gattgccatc aatgtagcag ccatcactaa gtattctcct
    21421 gcaacagcta gctcgagtgt tttcattgta tggatataat tcatatattg ctccgtgatc
    21481 gtagcgatcg gtatatcata tatatcgatt tccatttgct gaatcagatg aagcagcaga
    21541 tcgagaggac cctcgaatac atctaattta atattgatct ccgtcaactc attcactctt
    21601 tcttcttttt ctttgctagc cattttccaa tgtaaggctc tgtgatttca gttgcacgga
    21661 tggagacatc cgggtacttt tcctgtaatt catctaacat ttgaaagccc atattctcac
    21721 cgcgataaga cggattcaaa gaaatgtcat ggatgatcag ctgatcgttc gttgcccagt
    21781 aaataccaat aatcccttga atgttggttg aaccttctgg cgtaaagaaa aaaagttcat
    21841 aatcgtcttc agattcgtac gtatcaatct ccttcaacaa agaagtttta cctgtcagtt
    21901 tcttatggaa actcaatagt cccatagcaa ttttacggtt ttctttccgg taatgtgtta
    21961 acatcttctc accttcctat gactaagccc ttggaaaatg ctgcttataa acatccgtca
    22021 ttcttttctt cgtgatatgt gtataaatct gagtcgtaga gatatccgcg tgacctaaca
    22081 attcctgtac tgtccgcaaa tcagctccat tttctagcag atgtgtggca aagctatgcc
    22141 gcagcgtatg tggtgttact tctttgtaaa tcccagcctc tcgtactaac tgcttcagat
    22201 ttttccagat tccttgccga gaaagacctt tcccatgatt attgacaaat acatgcattt
    22261 cagatgcgtc ctttacaagt aatggacgtg cttcgtctaa gtagcgttcc agccattgga
    22321 tcgcataatc acctaaagga atgatccgct ctttatctcc ttttccaact gtctgtacca
    22381 gtccaagaga aagatgaaga tctcccaatt tcaatccaat caattcactg acgcgcatcc
    22441 cagtagcata catgacttcc aagattgcac gatctcttat ccctaacgtt tttgtcgtat
    22501 caggtgcttc aatcagcttt tctacttctg tcagagtcaa tgtacttggt aatttctgtg
    22561 ctttcttcgg cgtatcgata tgttgcatcg gatcatgatc agtgatacgc tcttgccgta
    22621 aaaactgatg gaatcttctg agacttgaga tcatccgagt aacagttgct gtcgcttttt
    22681 tttcttcatg caacttctcc agaaaacgga tgactaaaaa gcgatcgaca tcctgccatg
    22741 aagcaatttt ctgctcctgt agaaaatgca agtactgttc caaatcacgt tcatagcttt
    22801 gtcgtgtatt ggcggacaat ccccgttcaa tattcaagta gtggagataa tcgttgattt
    22861 gctctttcat aataatcccc ctcatgcctc tctatcatat cagataccgc ttcattctga
    22921 tagtcttttt ccataatctg agcaaagaaa aagccgtcta gacactcaga cggactggtt
    22981 tcttttt
//
LOCUS       DACDYI010000055        22266 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.055, whole genome shotgun sequence.
ACCESSION   DACDYI010000055 DACDYI010000000
VERSION     DACDYI010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.055"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(218..1015)
                     /locus_tag="IWM52_001957"
     CDS             complement(218..1015)
                     /locus_tag="IWM52_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAP9974204.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(1012..2196)
                     /locus_tag="IWM52_001958"
     CDS             complement(1012..2196)
                     /locus_tag="IWM52_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP9974205.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2221..3468)
                     /locus_tag="IWM52_001959"
     CDS             complement(2221..3468)
                     /locus_tag="IWM52_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAP9974206.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3683..5428
                     /gene="dnaX"
                     /locus_tag="IWM52_001960"
     CDS             3683..5428
                     /gene="dnaX"
                     /locus_tag="IWM52_001960"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAP9974207.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5448..5762
                     /locus_tag="IWM52_001961"
     CDS             5448..5762
                     /locus_tag="IWM52_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAP9974208.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5787..6383
                     /gene="recR"
                     /locus_tag="IWM52_001962"
     CDS             5787..6383
                     /gene="recR"
                     /locus_tag="IWM52_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAP9974209.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6447..7091
                     /locus_tag="IWM52_001963"
     CDS             6447..7091
                     /locus_tag="IWM52_001963"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAP9974210.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            7106..7435
                     /locus_tag="IWM52_001964"
     CDS             7106..7435
                     /locus_tag="IWM52_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974211.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7449..8387
                     /gene="holB"
                     /locus_tag="IWM52_001965"
     CDS             7449..8387
                     /gene="holB"
                     /locus_tag="IWM52_001965"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAP9974212.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8423..9247
                     /locus_tag="IWM52_001966"
     CDS             8423..9247
                     /locus_tag="IWM52_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAP9974213.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9240..9587
                     /locus_tag="IWM52_001967"
     CDS             9240..9587
                     /locus_tag="IWM52_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAP9974214.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9656..10528
                     /gene="rsmI"
                     /locus_tag="IWM52_001968"
     CDS             9656..10528
                     /gene="rsmI"
                     /locus_tag="IWM52_001968"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAP9974215.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10637..11758
                     /gene="dinB"
                     /locus_tag="IWM52_001969"
     CDS             10637..11758
                     /gene="dinB"
                     /locus_tag="IWM52_001969"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAP9974216.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(11812..12414)
                     /gene="nrdG"
                     /locus_tag="IWM52_001970"
     CDS             complement(11812..12414)
                     /gene="nrdG"
                     /locus_tag="IWM52_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAP9974217.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(12729..14918)
                     /gene="nrdD"
                     /locus_tag="IWM52_001971"
     CDS             complement(12729..14918)
                     /gene="nrdD"
                     /locus_tag="IWM52_001971"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAP9974218.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            15244..16011
                     /locus_tag="IWM52_001972"
     CDS             15244..16011
                     /locus_tag="IWM52_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9974219.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            16001..18043
                     /locus_tag="IWM52_001973"
     CDS             16001..18043
                     /locus_tag="IWM52_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9974220.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            18324..18473
                     /locus_tag="IWM52_001974"
     CDS             18324..18473
                     /locus_tag="IWM52_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAP9974221.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            18484..19998
                     /gene="dltA"
                     /locus_tag="IWM52_001975"
     CDS             18484..19998
                     /gene="dltA"
                     /locus_tag="IWM52_001975"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAP9974222.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            19995..21200
                     /gene="dltB"
                     /locus_tag="IWM52_001976"
     CDS             19995..21200
                     /gene="dltB"
                     /locus_tag="IWM52_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAP9974223.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            21245..21478
                     /gene="dltC"
                     /locus_tag="IWM52_001977"
     CDS             21245..21478
                     /gene="dltC"
                     /locus_tag="IWM52_001977"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAP9974224.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            21481..>22266
                     /gene="dltD"
                     /locus_tag="IWM52_001978"
     CDS             21481..>22266
                     /gene="dltD"
                     /locus_tag="IWM52_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAP9974225.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNN"
BASE COUNT         6958 a         4002 c         4613 g         6693 t
ORIGIN      
        1 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
       61 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
      121 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
      181 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
      241 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
      301 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
      361 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
      421 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
      481 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
      541 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
      601 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
      661 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
      721 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
      781 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
      841 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
      901 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
      961 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
     1021 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
     1081 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
     1141 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
     1201 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
     1261 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
     1321 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
     1381 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
     1441 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
     1501 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
     1561 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
     1621 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
     1681 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
     1741 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
     1801 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
     1861 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
     1921 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
     1981 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
     2041 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
     2101 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
     2161 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
     2221 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
     2281 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
     2341 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
     2401 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
     2461 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
     2521 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
     2581 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
     2641 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
     2701 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
     2761 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
     2821 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
     2881 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
     2941 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
     3001 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
     3061 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
     3121 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
     3181 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
     3241 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
     3301 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
     3361 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
     3421 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
     3481 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
     3541 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
     3601 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
     3661 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
     3721 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
     3781 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
     3841 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
     3901 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
     3961 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
     4021 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
     4081 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
     4141 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
     4201 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
     4261 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
     4321 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
     4381 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
     4441 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
     4501 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
     4561 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
     4621 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
     4681 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
     4741 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
     4801 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
     4861 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
     4921 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
     4981 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
     5041 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
     5101 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
     5161 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
     5221 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
     5281 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
     5341 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
     5401 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
     5461 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
     5521 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
     5581 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
     5641 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
     5701 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
     5761 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
     5821 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
     5881 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
     5941 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
     6001 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
     6061 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
     6121 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
     6181 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
     6241 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
     6301 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
     6361 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
     6421 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
     6481 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
     6541 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
     6601 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
     6661 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
     6721 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
     6781 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
     6841 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
     6901 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
     6961 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
     7021 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
     7081 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
     7141 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
     7201 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
     7261 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
     7321 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
     7381 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
     7441 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
     7501 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
     7561 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
     7621 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
     7681 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
     7741 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
     7801 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
     7861 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
     7921 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
     7981 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
     8041 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
     8101 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
     8161 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
     8221 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
     8281 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
     8341 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
     8401 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
     8461 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
     8521 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
     8581 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
     8641 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
     8701 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
     8761 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
     8821 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
     8881 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
     8941 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
     9001 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
     9061 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
     9121 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
     9181 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
     9241 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
     9301 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
     9361 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
     9421 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
     9481 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
     9541 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
     9601 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
     9661 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
     9721 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
     9781 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
     9841 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
     9901 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
     9961 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    10021 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    10081 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    10141 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    10201 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    10261 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    10321 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    10381 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    10441 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    10501 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    10561 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    10621 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    10681 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    10741 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    10801 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    10861 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    10921 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    10981 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    11041 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    11101 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    11161 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    11221 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    11281 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    11341 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    11401 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    11461 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    11521 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    11581 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    11641 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    11701 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    11761 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    11821 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    11881 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    11941 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    12001 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    12061 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    12121 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    12181 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    12241 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    12301 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    12361 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    12421 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    12481 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    12541 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    12601 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    12661 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    12721 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    12781 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    12841 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    12901 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    12961 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    13021 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    13081 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    13141 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    13201 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    13261 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    13321 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    13381 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    13441 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    13501 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    13561 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    13621 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    13681 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    13741 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    13801 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    13861 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    13921 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    13981 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    14041 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    14101 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    14161 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    14221 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    14281 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    14341 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    14401 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    14461 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    14521 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    14581 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    14641 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    14701 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    14761 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    14821 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    14881 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    14941 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    15001 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    15061 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    15121 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    15181 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    15241 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    15301 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    15361 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    15421 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    15481 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    15541 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    15601 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    15661 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    15721 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    15781 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    15841 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    15901 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    15961 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    16021 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    16081 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    16141 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    16201 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    16261 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    16321 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    16381 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    16441 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    16501 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    16561 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    16621 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    16681 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    16741 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    16801 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    16861 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    16921 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    16981 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    17041 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    17101 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    17161 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    17221 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    17281 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    17341 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    17401 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    17461 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    17521 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    17581 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    17641 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    17701 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    17761 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    17821 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    17881 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    17941 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    18001 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    18061 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    18121 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    18181 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    18241 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    18301 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    18361 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    18421 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    18481 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    18541 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    18601 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    18661 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    18721 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    18781 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    18841 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    18901 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    18961 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    19021 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    19081 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    19141 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    19201 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    19261 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    19321 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    19381 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    19441 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    19501 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    19561 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    19621 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    19681 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    19741 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    19801 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    19861 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    19921 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    19981 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    20041 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    20101 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    20161 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    20221 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    20281 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    20341 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    20401 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    20461 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    20521 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    20581 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    20641 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    20701 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    20761 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    20821 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    20881 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    20941 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    21001 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    21061 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    21121 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    21181 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    21241 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    21301 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    21361 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    21421 agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    21481 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    21541 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    21601 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    21661 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    21721 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    21781 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    21841 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    21901 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    21961 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    22021 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    22081 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    22141 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    22201 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    22261 aataat
//
LOCUS       DACDYI010000056        22239 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.056, whole genome shotgun sequence.
ACCESSION   DACDYI010000056 DACDYI010000000
VERSION     DACDYI010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.056"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            170..514
                     /locus_tag="IWM52_001979"
     CDS             170..514
                     /locus_tag="IWM52_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAP9974226.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            515..934
                     /locus_tag="IWM52_001980"
     CDS             515..934
                     /locus_tag="IWM52_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP9974227.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1026..1376)
                     /locus_tag="IWM52_001981"
     CDS             complement(1026..1376)
                     /locus_tag="IWM52_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974228.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1342..2673)
                     /locus_tag="IWM52_001982"
     CDS             complement(1342..2673)
                     /locus_tag="IWM52_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAP9974229.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2929..3495
                     /locus_tag="IWM52_001983"
     CDS             2929..3495
                     /locus_tag="IWM52_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9974230.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3640..3727
                     /locus_tag="IWM52_001984"
     tRNA            3640..3727
                     /locus_tag="IWM52_001984"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3672..3674,aa:Ser,seq:cga)
     gene            4061..5035
                     /locus_tag="IWM52_001985"
     CDS             4061..5035
                     /locus_tag="IWM52_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP9974231.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5360..7255
                     /gene="asnB"
                     /locus_tag="IWM52_001986"
     CDS             5360..7255
                     /gene="asnB"
                     /locus_tag="IWM52_001986"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAP9974232.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7450..7896)
                     /locus_tag="IWM52_001987"
     CDS             complement(7450..7896)
                     /locus_tag="IWM52_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP9974233.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8124..8273
                     /gene="rpmG"
                     /locus_tag="IWM52_001988"
     CDS             8124..8273
                     /gene="rpmG"
                     /locus_tag="IWM52_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP9974234.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8361..8879
                     /locus_tag="IWM52_001989"
     CDS             8361..8879
                     /locus_tag="IWM52_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9974235.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(8956..9912)
                     /locus_tag="IWM52_001990"
     CDS             complement(8956..9912)
                     /locus_tag="IWM52_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9974236.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10078..10884
                     /locus_tag="IWM52_001991"
     CDS             10078..10884
                     /locus_tag="IWM52_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9974237.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            10881..11870
                     /locus_tag="IWM52_001992"
     CDS             10881..11870
                     /locus_tag="IWM52_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP9974238.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            11867..12871
                     /locus_tag="IWM52_001993"
     CDS             11867..12871
                     /locus_tag="IWM52_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP9974239.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13003..13545
                     /locus_tag="IWM52_001994"
     CDS             13003..13545
                     /locus_tag="IWM52_001994"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAP9974240.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13565..14263
                     /locus_tag="IWM52_001995"
     CDS             13565..14263
                     /locus_tag="IWM52_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP9974241.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14284..14496
                     /locus_tag="IWM52_001996"
     CDS             14284..14496
                     /locus_tag="IWM52_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAP9974242.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14496..15458
                     /locus_tag="IWM52_001997"
     CDS             14496..15458
                     /locus_tag="IWM52_001997"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAP9974243.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15476..15871
                     /locus_tag="IWM52_001998"
     CDS             15476..15871
                     /locus_tag="IWM52_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP9974244.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16034..16399
                     /locus_tag="IWM52_001999"
     CDS             16034..16399
                     /locus_tag="IWM52_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAP9974245.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            16396..18207
                     /locus_tag="IWM52_002000"
     CDS             16396..18207
                     /locus_tag="IWM52_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAP9974246.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(18269..18436)
                     /locus_tag="IWM52_002001"
     CDS             complement(18269..18436)
                     /locus_tag="IWM52_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAP9974247.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            18684..19673
                     /gene="galE"
                     /locus_tag="IWM52_002002"
     CDS             18684..19673
                     /gene="galE"
                     /locus_tag="IWM52_002002"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAP9974248.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            19685..21169
                     /gene="galT"
                     /locus_tag="IWM52_002003"
     CDS             19685..21169
                     /gene="galT"
                     /locus_tag="IWM52_002003"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAP9974249.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            21193..22173
                     /locus_tag="IWM52_002004"
     CDS             21193..22173
                     /locus_tag="IWM52_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9974250.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
BASE COUNT         7212 a         3802 c         4629 g         6596 t
ORIGIN      
        1 aaaaaaagcg ttagcaacta tgcttaaaaa gcatatcgac tgaataaact aagcattgga
       61 caggctgaaa gactacattt aaaatgaaat gcgaagaaac aatagaaaat aaagttgcct
      121 tagaaccata gttgaatcac ctaacagtcg tgaagggaga ataagagaaa tggcagaaaa
      181 acaaaccgca ggacgagatc aattaggaga ttttgcacca aaatttgcag aattgaatga
      241 tgatgtttta tttggacaag tatggtccag agaagaatta tctgctcatc agcgtagtat
      301 catcaccata tctgcattaa tttcaggagg aaattttgaa caattgccag ctcatctgaa
      361 gattggcaag caaaatggca ttactagtga agaaatagca gaaatcatta cccatttagc
      421 tttttatgta ggatggccaa aagcttggtc agcttttagt ttagcaaaag aaatctattt
      481 agaagaaaat agcgaagata agaaagaggc gtaaatgacg acaaaacacg aagaaataca
      541 caatggcgtg atttttccag caggtgaaaa aaacgaagct tacgctcaag tatttaccgg
      601 gcaaagctat ctgaatatgt tagtgaatga tcccgaagtt tctgttggtg taggtaatgt
      661 aacttttgaa ccaggttgtc gcaatcactg gcatgtccat aaagacggct atcaaatctt
      721 acttgtgacc ggaggagaag gctggtatca ggaagaagga aagccagcaa gacgattaat
      781 ggctggagat gtcgtcgtaa cgcacaaagg gattaaacat tggcatggtg cgacaaaaga
      841 cagttggttt gaacatttgg cgatcactgc tggtaattca gagtttttag aacctgtaac
      901 agatgaagtg tatgaaggat tggaaaatca ataatgtcaa gcttgaacag ctcctgaaag
      961 tagattttaa tcgaactttc aggagctgct tttttgattc agtcttttat aaatagaaaa
     1021 tagggttaat aataggaatg agaagcacgt cgaaagatct taatattgat aatcggctgg
     1081 gcatacatga tttccacgtc atcaatcaaa ctacctaaca gatgataatc ttcccgatca
     1141 tgtcggggat tacctaatag ttcttgataa tagatttcca tactagggtc ccgaggattg
     1201 ttcagcaaat cagcaagtac atctaaatct tctggctgag tttctgccgt accctgtatc
     1261 tctatgaaaa gcccttcctc agcaaattct actccaatac gtatttcagt tatttcatgg
     1321 gaatataaat aagtaagaag ctcatcgatg attctttttg ataattcttt ttccaagtgt
     1381 ctttttttcc tttctgacag cggtaaagtt atctagtatc ggtacaagga tacctgcaac
     1441 gaaaccagtt gaaaaaccac tgttatatag atttagtcct ccgtgcaaag tgctgacgtt
     1501 atggacaaga gcaatatgta gtacaccagc tattacaccg taaaaactgc cgtaaaaacc
     1561 gcttatgggc gcgagacctg tcccaaacac agctgcaaca agcatgccag tactggtgat
     1621 ctcatgaaaa gttccaaaca aagaagcaat aaatattccg actagtatcg gaaaactgtt
     1681 ttttaaatga caaccaaaag cactgaaacc caccgcagat aaaatagcac cgatgacagg
     1741 tccatttaac tgaccgccaa ccagcaaaac ataagaagac agcatcagac caactaagcc
     1801 tatgttgatc aaagtagcac caataccaga attagcaatg aaatcagttg tcaattttcc
     1861 agatgaatcg aaaatttctc gaatccccaa aagccgaaag gaattataat agaaaccaat
     1921 tccgactgtc agcgaaaaaa agccaaaaat aatccaaatg agaggaaagt gatattcatt
     1981 gaaaaccaat gtattttcga cgattgaata gctgaataac ctcaaaacag cagtaaaagt
     2041 catcgcaatc aacccagagg tgaatccaat attataaagt gtgaatccac gatggaaaac
     2101 taaagtttga gcagctaagg gcggaagaag tagaccaatt gctatgccag ctaaattccc
     2161 caataaaata cctatcagta aaggaaaacg cataccaaaa gtgatataac taacgaccgg
     2221 tgataatgcg ctaccaaaca gtccgatcat gatgtactga gaaaaaggtt tattgacaaa
     2281 tacagcgtat agatacacac ctaaaatgat tgagatagaa ttataaaggt ttttgccaaa
     2341 aaaagagaaa ccggtaactg ttagaatcga agcaaataag ggaccgttta aaatcacctt
     2401 gtgccgataa gctaaaagca cagaaagtaa ggtcaaaatc cctacattta tgaatgcgct
     2461 tccgaaaccg cctaaagcaa aataatctgt caaaagattg cttggcgagg taagaatagt
     2521 tagcaatcca ctccaattat cagtaggagt attagaaagc aagccaataa aaagaaaaaa
     2581 aacacctgta aaaccaagat aaaaatactt tcttttctgt gaaacgattc gtttttgagg
     2641 atgaatataa ataacgtcag tttcgttttc cattacacac cttctttttc attttcttta
     2701 actataactt gaaatgaaag atttggacaa ctatcattta ggtcaaaact aaaaatgtaa
     2761 gggaacccat tgaatttaac caaatttttt taattttgca aaaaaaagtt aaagaagact
     2821 gaaaaaaaat ttaaaatata acgttttgag taaaatgttt gcaaaaaatg ctaaaaatag
     2881 ggtacaataa aacaacatat actgcagtaa agaagtgggg gagttttaat gagaagaaaa
     2941 gtctatacca aggatcaaat tctaaaagct gcttatgatg ttgttgcaaa ggaagggttt
     3001 aaaggattta ctgcgagaaa tattgctaaa aagatgggga tttcgacaca gccgatttat
     3061 ctggaattca aaaacatgga agatttaaaa aatacgttgc tggattctat ctttgaacaa
     3121 ctaaaagaag atgttttccc tattgaacgt actggtgata aaatcattga tctcggtttg
     3181 aactatatct actttgcaaa aaagcatcat agtctttaca tggcattgta cattgatgaa
     3241 catggggacg gaaagaaaat gcatgatttt tcatacaatt atttccgtga aatagttaaa
     3301 gaagatgaaa gatacaatca attatcggat gaatatgtcg atgctctgca tactggtaca
     3361 tgggtagcag tgactggctt agcgtcattg atgtcatcaa acatcatgca tccaacagaa
     3421 gaacaaatca ttgcactgat caaacggaca attgactcga ttttagcatt agataatcct
     3481 gaaataatca gatagtgttc tatcagtatg aatttgatcg gtaagtccgt tggctgcaaa
     3541 ctgtcaatgg acttttttgc taaaaaaagc gatttctgtt atttatctat tagaaatagg
     3601 cttgacattt cgtgaaaaaa gtttaaaaat agtacttacg gaaggttggc agagtggtaa
     3661 tgcaccggac tcgaaatccg gcgaaccggc taataccggc gcgcaggttc aaatcctgta
     3721 ccttccttat atcaacgatt agaggttgtg aagaagctgt cctgcatcaa gtaataagaa
     3781 cggccaaaca aaaatagctt ttcctatttt ttcgtttgat tgggcttatt accgcagatg
     3841 caagcttttg aacaccgttt gttcggaata aggggctgtg acaagacttt tgtcacagcc
     3901 ccttattgtt gtacttttaa cctacaatat tttttgattc cccctgagta ctatttttta
     3961 taaatgctag taaatcaatc ttctttggcc tttttttgat aaaaaaaata ctagatgtaa
     4021 gataaaagtg taaaaacgat ttgttttagg acgggaaacg atgtgtaccg caatcactta
     4081 tgcaacgaaa gaccattatt ttggaaggaa ttttgattat gagatgtctt ataatgaagt
     4141 ggtcactatt actccaagaa attatcgttt cgattttaga aaggtaaaaa atttagataa
     4201 acattatgca atgataggaa tagcagctgg ggtatccaac tatccactct attatgaggc
     4261 tacaaatgaa aaagggttga gtatggcagg actgaatttt cctggcaatg ctgattataa
     4321 agaattacaa gaaggaaaag ataacgttgc accatttgag tttattccat ggatactagg
     4381 gcaatgctca accatagacg aagctaaaga attattggct actatcaatc tagtcaatat
     4441 cgattttagt gaaaaactac ccttgtcacc tttacactgg ttattagctg ataaagaaaa
     4501 atcgatagta attgaaagta tgaaagatgg acttcattta tatgataatc ctgtcggtgt
     4561 gttgacaaat aatccgccat ttgattatca attatttaat ctgaacaatt atcgctcgct
     4621 atcaaatgga acaccagaaa atcatttttc aaaccagatc agcttgaatg tttatagtcg
     4681 tgggatggga ggattgggct tacctggaga tctctcttct gtttctcgat ttgtaaaagc
     4741 cacatttact aaaatgaatg ctgcatcagg tgattcagaa tctgaaagta tcagtcaatt
     4801 ttttcatata ctaggctcag tcgagcaaca aaaaggagta tgcgatactg gagaaagaaa
     4861 atacgaatat acgatttatt cttcttgctg taacgttgat aaagggattt attattatcg
     4921 tacgtatgaa gacagtcaaa ttactgctgt aaatatgaat aaagaagact tggatcgtca
     4981 tgaattggtc agttatccag tcataaaaga gcaacagata aactatgtca attaattaat
     5041 gaatagatcc gtttggtatc atattttctg atacagtcat aattagacac gatctttagg
     5101 tcgtgtcttt ttttgctggt ttttttggaa atagcatcca ctaatatttt tttaacacga
     5161 agagaataag gtagcctcaa aaaagaaaac atggtaaatt agatattggg actttatttt
     5221 ctgtaaaaac agaaactttc atcttttgtt ttcacgtgac gaaattgtaa gagagaaaat
     5281 attcgtccgc tatttattct atgataagat gcatatgaat gaaataaaca gtaattttat
     5341 attaggatgt gaaagttaaa tgtgcggtat tgtaggtttt gttaacgaca aggacaacaa
     5401 aaaaacaatt attaatacaa tgatggatcg aatcgttcac cgtggaccta atagttcagg
     5461 agaatatatc gataaacatg ttgctttagg attcagaaga ttaagtatta ttgacttaga
     5521 gggcggtact cagcctattt acaatgaaga tcggacaaag atcattattt ttaatggcga
     5581 aatctataat taccagcctt tgagagaaga attgattgca gctggccacg tttttcaaac
     5641 acatgcagat acagaagtcc ttttgcatgg ttacgaagaa tggggaacag aattattgca
     5701 aaagatccgc ggaatgtttg cttttgctat ttgggataat gaaaagaatg agttattcgg
     5761 tgcaagagac cactttggga tcaaaccata ttattatgca gaaatgaacg gaacatttat
     5821 gtttggttca gaaatcaaaa gttttctgcc tcatcctgat tttaacaaag aattgaacaa
     5881 agaagcgttg aaaccttata tgactttcca atattctcca ttaaacggcg aaacattctt
     5941 caaagatgtc tatcgtttgc ctgaaggcca ttattatacg tataaagatg gaaaaataga
     6001 cattcagcaa tattgggatg cagattttga aacaaaagaa acgcattccc gacaagaatg
     6061 gatcgaaaaa atcgacgaaa ccgttcaagc ttctattgaa gcacatacgg tcagtgatgt
     6121 agaagttggt tctttcttat ccagcggggt tgattccagt tatgtaacct ctgtattgaa
     6181 accagatcat tctttctcaa tcgggtttga tgataaaacc tataatgaag cgattgaagc
     6241 cagaaaacta accgaactat tggacttaga taatacagca gcagtgatcg acggagacat
     6301 gtcgttcaaa gcattccctt tgatccagta tcacttagat gaacctgatt caaatccttc
     6361 ttgcgtacct ttgtactttt tagcaaatct tgcttcacaa agtgtgcggg tagtccaatc
     6421 tggagaaggg gcggatgaac tgtttgcggg ataccaaacg tatggattcc atacgaattc
     6481 aaaattcatc cgtgtgattg ctcaaggatt gaaaaaatta ccaaaaggaa cacgctataa
     6541 tttaggacgt aaaatcggaa aaatgaaaaa tttccatgga cggatccatt tatatgaatc
     6601 acttgctcct gcaaaagaat attttatcgg tcatgctcga gtgttcgaag aatcagaagc
     6661 atcagaagtg ttaacaccaa aatatcaaac agcaccatct gtggatgaaa tcatgactgt
     6721 tcattacgag aaaacagaag gaatcaaaga tgaagtcaat aagatgcaat atgtcgatct
     6781 tcatcaatgg atgccaaaag atatcttgtt aaaggcagat aaactttcta tggcaagttc
     6841 tttggaagtc cgagtacctt tattggatat cgaagtcatg aaattggctc aacaaatacc
     6901 aagtaagttt ttattgaatc aaaacaacac aaaagatatt ttcagacaag ctgcgaacaa
     6961 acatcttccg gaagaatggt cgaatcgtgt gaaattagga ttccctgtgc caatcaaagc
     7021 ttggttgaaa gaagaacacg gctacgaaca agtcaaagca ttattcgaag cagattttgc
     7081 aaaagaattt tttgatcaag aaaaaatcat gaagttgcta gatgaccatc acgaaggacg
     7141 taaagaagag caaagaaaaa tctggacaat cttcagtttc ttgacatggt ataaagtcta
     7201 ctttgtagat gaaacgattc cgcaagcaga agcaatcgac tatgtgactg tttgatgaaa
     7261 atcaaatatc aataaaaaag aggttttctc ctaccgttta acactgagtg ataagaaaca
     7321 atttcaaaaa acagctcttc ccgttttata gatattatga cttatggttc ggagttggca
     7381 gaggaacacc attgtttcga acataagggg gttgtgacac gattttcgtc acaacccctt
     7441 tacatatgtt caataatcga tttctttttc ttttgtccca tcttttttat aaatgaacaa
     7501 agttgattct ttgttttgtg ctacttgctt tcctttttcg attgcttctt tttttgtatc
     7561 aaaatggttg cttgcctttt ttgccccttt agatttcacg atccattgtt cttcctcata
     7621 ttctacgatc acatctgaat caagcagttt tccagcacga ggattcgtat cgtgttcatc
     7681 cgtcttgctt ggattttttt ctttttcgaa agttcgtttt tctgattcac tagcatcttc
     7741 ataccatttt tttgcttgat caatggcaat tgggatcgca cggttatcag ggtacccttc
     7801 atctaataat gcatttgcga tgtcgattgc tttttttctt gtcaatttat ctaagttttt
     7861 cattgatgca ggaaagtcct tcatgttcca tggcatatta gtatccccct ttattaaaaa
     7921 acataatcta tataaacaag tgtaagggta tttatacaaa aagaaaaaga ttttgtttga
     7981 gaagtaaaac cgttgtattt atcgtcaagt agttttactt aacaaaatcg tgaaaaacac
     8041 tagcaatcta gaaaaaatca tggtaagata atcaagtctg agagtcatct tagaggattc
     8101 ttgtaaaaat gggagggaaa aacatgcgcg taaacatcac tttagagtgt acttcttgta
     8161 aagaacgtaa ctatctaaca agcaaaaaca aacgtaacaa tcctgatcgt ttggaaaaac
     8221 aaaaatattg cccacgcgaa cgcaaagtta ctttgcaccg tgaaacaaaa taatgttttt
     8281 aaagttctca attccttgct ggtcaaagaa ttgagacttt ttttatctca aaacatctaa
     8341 aatcataagg agcggataat atgtttattt ccatgtgggc acaagataaa aatggtctga
     8401 tcggcaaaaa tggtttgtta ccatggcgat tgccaaatga tatgcgtttc tttcgcgaac
     8461 acacaatgga tcgaatttta gtgatgggaa gaaagaccta tgaaggaatg ggggatcttt
     8521 cacttcctta tcgccatatc atcgtgttga caacgcaacc aaattttcgc acgaaagaaa
     8581 atgcggaagt aatgcattcg atcgatgaac ttttgtcgta tagtcaaacg atctcagaag
     8641 atatctatgt ttcgggtggt agtcggattt ttcaagaact tcttccttac actggtcgga
     8701 tctggcgaac attgatcgat agcacgtttg aaggcgatac gtatattggg aatattgatt
     8761 tttcagactt cgcccttata gaggagtatg aggggataac ggatcaagaa aatctatatg
     8821 cccatcgttt tcaaaaatgg gaaagagtaa aaaatagggt tgcttcaaaa gaagaataaa
     8881 atctatgcgt ctagtttgtt aataagaaaa aaagcatctc acgactaatc gtgaagatgc
     8941 tttttttagt taaaatcaat ttagtttttc tttgatatca ttgaccattt cctgactggc
     9001 aataggtccg ttatatagcc aactagattc aggtccgaat tgcagtacat gattgtctgt
     9061 cactgctttt agattcttcc agcttggttc attgaacatg gcagcatctt tgtcactgtt
     9121 cacaaggatg atgtagtcag catctaattc agctagcttt tcagaggaaa cagcagacca
     9181 gtcagaagtg gcttttttgg agacctcttc cactagatca ggaacaccaa attccagatc
     9241 gtcgtatacg attcttccac ttgaccgatt tttgctgacc ataaaggctg agttgttggt
     9301 cacccacaac actgcagctg tcttgttctc gattttatct gctagttctt ctcgagtttt
     9361 cttgatggat ttttgataat ctgaaatgat tttttctgcc tctttttctt ttgccaaagc
     9421 tttgccgaca tcttttagtt gttcttccca tgtgacatct gttccgtttt ttacaacata
     9481 agttggcgcg attttgctat attgttcgta tttcccgcct tcgacagtag cggaagaaga
     9541 aatcaacagc aaatcaggct caaagcttaa cactttctca taaggaaggt cataagaaat
     9601 cgttgggaca tcctttaaat catcttgcag ataatcttga atggaaccac tgccaacagt
     9661 ccactgggcg ataggttttt cccctagtgc aatcagatag tcttctaagt aactgccgat
     9721 gatccgtttg ggcgcgtttg gaatctctac tttatgtcca agtgtatctg tgactgtatg
     9781 agtactattg gatgaagtcg tcgaattggc agttgtagct gtttctttat ctgaagaggg
     9841 ggagcaagcg ctcagtactc cagccagcag caatatgcta gataatgtga atacttttgt
     9901 ttttaatttc atttctttcc tcctaacata attgagaatc tttctcattg gttattattc
     9961 ctgatatttc gaacgctgtc aaccgcttca tttggctata gatatgaaag tagcgcttga
    10021 cgaaatgttg aaacagcgtc ataattgaga atgattttca gcgaaaggat gggtgaaatg
    10081 acgagattaa gagcagctga cattgcagcc ggctacgaaa acaaaaaaat cattgaaaac
    10141 atgtctgtta cgattcctga agggaaaatc actagtttga ttgggccaaa tggaagtgga
    10201 aaatctactt tattgaaaag ttttacccgt atactacctg tgaaatcagg aaagatcatt
    10261 gtcaataact ctgatatctc tacatacagt tcaaaagaac ttgcaaaaaa aattgcatta
    10321 ttagcacaaa ctagtgaaca ccctacaggt atgacagtag aagaagtagt atcttacgga
    10381 cgataccctt atcaaaaact gttcagtggc atggacacgc aagatcatga agcaattgag
    10441 tgggcgttgg atgctacagg tctagctgga tttcgtatgc gggatatcgc aacgctgtct
    10501 ggtggacaaa cccagcgtgt ctggatcgct atggcgcttg ctcaggaaac agatatcttg
    10561 atcttggatg aaccgacaac atttttagat ccggcacacc aattagagat cctacagtta
    10621 ttgactgaaa tcaatcaaat gaaacaaaca acgatcttga tgtcgattca tgacattaat
    10681 catgcttcac gtttttcaga ttatttatta gcaatgaaaa acggtaagtt gatcacagaa
    10741 ggtacacctg aagaagtgat cacaaaagag tgtatgaaaa aaatatttga gattgatgca
    10801 acgattgtca cgcttccaag aacaaataaa ccagtttttc taacctatga tttgttgcca
    10861 aaagagcagg aggatcaatt atgaggacta tgaagaaaaa tagactcctg cttttggtct
    10921 gttttgttct tttgattatt ttttttgttg ctctttcaaa tggcgcagcg actgtgggaa
    10981 aagcagattt ttggcaggcg ttgtttgatt ttgatagatc aaaacaaagt caccaaatca
    11041 tacgaaatat tcgtttgcct cgtgtggcgg gagcatttct tgtagggagt tgttttgcat
    11101 tgagcggtgc attgatgcaa ggagtgacaa gaaatccttt agcagattca ggcttactag
    11161 gaatcaacgc gggtgcttcg ttggcaatgg cccttagttt tgctttttgg cctaaagctt
    11221 ctgatttttc tgtgtttcta atgtcattgg caggttcttt tgctgtaact ttgctcgtat
    11281 ttggtttttt gcagttttcg aaaatgggca tgaatccttt acagctgatt ttagctggcg
    11341 tagctattag ttcttttttt actgctatca gccaagcact tacccaattc tttaatctgc
    11401 agcaggatct tgctttttgg tttgtaggcg gagctgccaa tatcacatgg aaacaattag
    11461 cagtattgtt tcctttatat atacttgctg ttttggtttc tttttcatta ggaaaagcaa
    11521 tatctttgat tcatttgagt gaaacacatg cattagctct tggaagtcat cctaaacgaa
    11581 cgcggttgat tgttttctgt ctagttgcag tgttagcagc cattgccgtt tcgttagtcg
    11641 gacctgtttc atttattggg ctgttggttc cgccagttgt tcgcggaatg gttggaacgg
    11701 actatcgtta tgttctacct gtctcttttt tagttggcgg tggattggtt atgttggcgg
    11761 attttacagc gaggatgatc aatccccctt ttgaaacacc ttttggatta gtcattgcat
    11821 tgattggcgt tccgttttta ctgatacaag taaggaggga acaacggtga aaaaaatata
    11881 ttggacgttc tatttcttgc tttttgtact tgtcatcctg tctataggta gtttaatgat
    11941 tggtacgcct tttatttacc tggatcagct ttttgccacg ttcataggga aagaatcatc
    12001 ttctttggcg ctgattgtta gagaatttcg agttcctcga ttactgatca gtttattagc
    12061 aggagcttgt cttggcgttt ctggttatat cttgcaagga gtaacaagaa atgaattggc
    12121 agattctagt atattgggca ttaatgccgg agcaggattt ttagtgatgc tttatttagg
    12181 tttcttttcg cactattcgc tgccgtttct attgctagtt gtagcctttc taggtggtgt
    12241 tttagctgct tgctgtgtct atcttgcggc ctatgatcga aatggctttt tgggtatgaa
    12301 tcgaatctta ttgtccggta ttgccgttaa tgccggcctt tctgcattaa cattgctatg
    12361 cacgatccaa ttatctaagg aaaattatgg atttgtgaat gcatggttag cgggatcaat
    12421 ctggggtgcg agctggcctt atgtttgggc attgcttcca tgggcggtta tcttaattcc
    12481 gctaggagga ctttatgcac agaggattgt attgctatca tttggtcaag aacgcgcgca
    12541 aggattaggc cttaacgtgg ctaaaagtca aaaaatcctt ttgttgttag ctgttgcttt
    12601 ggcaagcggt agtgtagcgg tgacaggtag tttatctttt gtaggactac tagctcctca
    12661 tgctgcgaag ctagtcgtac gtaaagaaaa taactggacc tttgttctga gcggtttgtt
    12721 cggttccatt tttctgctta gtgcagatat ggttgctaga gtgattctac taagtgggga
    12781 agtacctaca ggaattttga ttgccctttt tggtgcgcca tattttctgt atgcactttt
    12841 tatcagacaa aaacacgcgc tgaacagttg aaattcatta aaaaaggaag gccaatcatt
    12901 tctaggtctt ctttttattc tatttatgca tttatttgaa agttcgattg ctatttttct
    12961 ttttttccta taccatttta tgagagaagg aagggtgagt ttttggacca attgaaagta
    13021 agattacgcc ggaaaggaat cgaaaagctg aaaaatctgg ccaaaagtgg caagaaaaag
    13081 gaaaaagaac agcgaatcta taaacagcta ttcgccagcg agtactggaa gtcagcaaaa
    13141 gtgataggga cgactttatc gaatgaattt gaactggata cacagccggt tatccaacga
    13201 gcagaaaaag aacaaaagaa aatagttgta cctaaaactt tgccgaaacg tcagatggcg
    13261 ttttatgaaa tggatgaaca gtccgttctt gtacgttcga gttttggtgt tcttgagcca
    13321 caatcagaga agctctattt gccggagatg atcgatctcc tcattgtacc aggtattatc
    13381 tacaatagag ccggttatcg aatcggtttt ggcggtggtt attacgaccg gtatttaaat
    13441 aattttcata aaaaaacatg tagccttgtt tttcgtgaac aattgaatga acaatggcaa
    13501 ccggagagct ttgatcagaa gatccaacaa ttgtttattg actaaaatga aatgggaggt
    13561 tttcatgaat tatcaacagc aaataaaatt gcgtgcatgg ttaagacgac cgttatggac
    13621 ctatgctttt ttagggatac agacagcggt cttcctcatg atggaattat ttccgcgact
    13681 tgagatccct tattatgccg gaatgtatgg tccttatctt gttcacttca atgaatggtg
    13741 gcgtttagtg acaccaatct tcatccattt tggactgatg catttcgtaa tgaactcact
    13801 gatactgtat tttatggggc agcaaatcga ggcaatctat ggccattggc gttttttctt
    13861 gatttatatg ttcagcggga taatgggaaa tacagcaagc tttgctttta acgaagcgaa
    13921 tgttctgtct ggtggtgcaa gtacttcgat attcggtttg ttcggtgcgt tgtttatctt
    13981 agggtttcat tttaaacata acccagcgat ccagcagcta gtcagacatt ttcttttatt
    14041 tattgtgttg acatttgttt ttggtcttct ggatacctct gtagatgttt ggggacatgt
    14101 cggtgggcta attggcggat tagtgttagg gaatgttttg ggtctaccga aacaacagac
    14161 aagctattcg atacaccaaa ggattctttc tacactagtt tttgtatttc tttttgtaat
    14221 atgtatttta ctgggtttaa aaaaatatgg tttacttgta taatatgaag gaagtgctta
    14281 aagatggaga gcttatatga tgtacagcag ctgttcaaaa aatttggcat ctttatctat
    14341 gtgggtgcac gcatctatga cattgaatta atgactatcg aattgcgtaa tctgtatgat
    14401 agccgtttga ttgatcatga tacatacatg acagcgtggc gcatattaaa aagagagcac
    14461 cgaattgaag aaagccgaaa gaaaggaaat ttgtaatgga caagaagatt attgggattg
    14521 atttaggtgg aacaacagca aaatttgcaa ttttaacacc agaaggagag attcagcaaa
    14581 aatggagcat cgataccaat attttggatg atggtaagca tatcgttcct gaaatcattg
    14641 aatcaatcaa tcatcgtttg aatttatata atatgaaagc cgaagacttt atcggaatcg
    14701 gcatgggtac cccaggaagc gtggacagcg aagctggaac ggttattggt gcgtataact
    14761 taaactggac agaagttcaa tttgtgaaaa aactgatcga ggcaggaaca ggaatcaaat
    14821 tcgctatcga taatgacgcg aatgttgctg ctttaggtga acgatggaaa ggtgctggag
    14881 aaaacgatcc agacgttgta ttcgttactc taggaacagg tgttggcgga ggtatcgttg
    14941 ctggcggaaa tttgattcac ggcgtgagcg gagctggagg agaaatcgga catatcactg
    15001 tggatcctga agggttcgaa tgtacttgcg gtaaacgcgg atgtttagaa actgtatcta
    15061 gcgctacagg tgtggtacgc ctaggtcgtt tcttggcaga agaatacgca ggggattcaa
    15121 aactgaaagc aatgctagac aacggagaag aagtaacaag taaagatatc tttgaaatgg
    15181 ctcaagaaga cgatccgttt gcattgatgg ttgttgatcg cgtatgcttc tacttaggtt
    15241 tagcttgcgg aaacttgggg aatacattaa atccgtcaag tatcgtgtta ggtggaggag
    15301 tatcggcagc aggagaattt ttacgtagcc gtgtagagaa atattttaaa gaatttactt
    15361 tcccgcaagt aagagaatca acaaaaatca aattagcaga gcttggtaat gaagctggtg
    15421 tcattggtgc tgcctcatta gccttgaaat ttgcttaaaa aaacggaggc attacatggt
    15481 tttatgggta attaatatca ttttgctatt gattgtgctt gcaatcatcc tttgggaagt
    15541 ctatctacga gtaatggcca aacgttcagc aacgactttg acagaagaag aatttcgaga
    15601 aggaatgaga aaagcacaag taatcgatgt tcgcgaaaaa gatgtgtttg atgctgggca
    15661 tattttaggt gcaagaaaca tcccttacac tgttttgaaa gattcattag gatctattcg
    15721 aaaagaccag cctgtctata tttacgatca aaagaaaagt ttgagcatcc gtacagcaaa
    15781 taaattacgt aaagcaggtt atcaagagat ttatttactt aagggtggat atgacggatg
    15841 gtctggaaaa atcaaaagta aaaaagtata aaacagatga ttgtatgaca gctttaaaaa
    15901 ccgaatcaat cattagattt tgaccaagat cacatgaacg gcgcaatgga tagttgtgcc
    15961 gttcattttt tttattttgt gctattatct acatatataa gctaagcgaa aaggtagaaa
    16021 gaaggaaaca actatgatca aattaaaacg tgtttacctt gatgctgaaa aagaggatgg
    16081 ctatagaata ctcgtggata gattctggcc acgaggaatt tcaaaagaaa aagcgcagtt
    16141 agatctgtgg ttgaaagaaa ttgcacctag taatgaagca cgcaaaactt ttgaccatgt
    16201 accagaacga tttgcttcat ttgcaaagaa ataccagcaa gaattgattt tcgaagaaga
    16261 aaagaagcaa gctattttta aactcagtca acttatgaaa gaacatccag tcattacttt
    16321 tgtttatggt gcaaaaaatg aacatcagaa ccatgctgtt ttattgaaaa aatttatcga
    16381 aatggaggca tgcaaatgaa aatagggatc attggtgccg gacctagagg attaagtgta
    16441 gcagaaagga ttctccataa taatcaggac agacattctc ttgaactttt tcttttcgat
    16501 cccaatggtc ctggaggtcg agtttggcgg ttgaatcagc caccggagtt attaatgaat
    16561 tctgtttctc aacaagtgac gctttttaca gatgaaacat tgtccagtgg aggattggta
    16621 acacagggac ccaacttgtt tcaatggagt aaaggacctg ctacagatta tatcgaacag
    16681 acggtaacca aaaataagca actaattata gaagaaatca atcgtctgga aaaagatcag
    16741 caatctactc gatgtttata cggactctat caacgctggt ttttctcaaa gttgcaagaa
    16801 gaatttccag cttctattca gtttgtttat tctttagtaa aaaaagtaag caaaagaaaa
    16861 gatggttatc ttttacagac agatcagcaa agctattcta tcgatcagct tgttttagca
    16921 agcggtcact gggaaaatga tgagacagaa gaagaaagag tgtgggtaga ctacgcaaag
    16981 aaaaatgttt tactgtatca gcttccttct aatcctgcag atgtagatct ttcgaagata
    17041 ccgcctaaag aaaatgtctt tttaagaggt ctaggcttaa gtttttttga ttatgtcgga
    17101 ttgtttacag tatcaagagg agggcgcttc gagaacgaaa agggaaaact ggtctatcat
    17161 ccatcaggca aggaaccgat tgtatacagt ggatcaagaa gggggctccc ttattaccct
    17221 cgaggaagaa atcaaaagca aggcggagca atggcatggc cacggttact aacaaaagaa
    17281 aacctggagc aatggcatca gtcgggattt ttgactggcg aaacattttt tgactatctg
    17341 aaaaaagacg ttgagttgtt ttattacaag aaaatcatcg aagaatatca cttgccgatt
    17401 ccaaagaaaa attttgagca tgattttctc tccctctctt cagaggagtg tctcggaaag
    17461 catccagcac taatccctta tagatgggat tggtcgtatt tagaaactcc tcttgcctat
    17521 cagaatgagc ccttttcaga agctagccga atatttattg aagggcagat aaaagaagcg
    17581 gaaaaaggca attgcacagg agcatttgct tcaacatttg atgcattaaa agactggcgt
    17641 gatccaatcc ggcaggctat tgaatggcac gttttttctg ctaaggaata caaagaaata
    17701 ctatggggat ggtttactca tctaaatagc tttttaacga tcggtccgcc aatcattcgg
    17761 acgaaagaat tagcagcact gatagatgcg ggtatttttc atttgatcga accgccattt
    17821 gatattcagc aaaagcacaa ccgctatgtt atttctctac aagataaaga aatcagcagt
    17881 cgctatttga ttgaagctcg attgccacaa acaagctttt ctcgttcaag taatcctgtc
    17941 ataaaaaatt tgaaggaaaa caaacttgtt cgcccttttt cattttcaga taatacagga
    18001 agttatcatt cagaagctat agatgttgag cgaggtacga atcgcgtgat tgatgcttca
    18061 ggggaaatcc agtctgactt ttattgctat ggaatacctg ttgaaggtgt ggattggcta
    18121 actgcagcag tagctagacc ttatacagat ccatggaatc tgagacaggc agataaaata
    18181 gcacaactta tattgactga caaataaaaa tacgcatgat gacttatctg ttttttagaa
    18241 agtcttcatg cgtattttta aataattatt aacgagaaac gcgtttacgc attgcttttt
    18301 tacggttttc ttcgatcatt gcttcttttt cgtcaattgg atcgatcact gtttttttca
    18361 cagaagcaac caaaccagca acggcagcgg ctgttgccag acttcctact aatattccag
    18421 atacaaattt tttcattgaa tccacctcat ttattatttg ttatatctat tatggaacaa
    18481 aagagaaaaa aaggctagtt ttacgtttgt ttgtaaacct ttgcaaaaaa tccttgaaaa
    18541 tgtttactaa atgttttatt ataaatttac catatgaatt tacgaaattt tactttgttt
    18601 gttttcacca taaaagtaaa cttcgttttt taaattatat agaatcaaca aacacaattt
    18661 tactaatcaa aggagtgttt tttatgacaa ttttagtact aggcggagcc ggttatatcg
    18721 gttcgcacgc tgtagaccag ctagttcaaa aggggtatca agtagctgtg gtagataatt
    18781 tgctcacagg tcacaaacaa gcagtacatc cagatgctca tttttatgaa ggagacatcc
    18841 gggataaaga atttctacgt tccgtatttg aaaaggaacc gattgaagga gtaatccatt
    18901 ttgctgcaag ctctttagta ggagaatctg tcgaaaaacc attgatgtat ttcaacaaca
    18961 acgtatacgg tatgcaaatc ttacttgaag tcatgcacga gttcaacgtg aataaaatcg
    19021 tattctcttc tactgcagca acttatggag agccgaaaga gtctccaatc acagaagata
    19081 caccagctaa tcctaaaaat ccgtatggtg aaagtaaatt gatgatggaa aaaatgatga
    19141 agtggtgtga ccaagcatat ggcatgcgct atgtcgcttt gcgctacttc aatgtagccg
    19201 gagcaaaagc agatgcatct atcggagaag atcatacacc agaaacgcat cttgtcccaa
    19261 tcattttaca agtagctctt ggtcaaagaa aagccttggc tgtttatgga gacgattatg
    19321 atacaccaga tggaacatgt atcagagatt atgttcaagt agaagatttg attgctgcac
    19381 atattttagc tttggaatac ttgaaagaag gaaatgaaag caacttcttc aatttaggca
    19441 gcagcaaagg atactcagtc aaggaaatgc tagaagcagc tagagaagtg acaggaaaag
    19501 aaattccagc agaaatagca ccaagaagag ctggagatcc tagccgatta gtcgcttcaa
    19561 gtgaaaaagc gagagaggtt ctgggttgga aacctgaata cacagatatc aaagcaatta
    19621 tcaaaacagc atgggattgg catgtcagcc atccaaatgg ttatgaagaa taggggaatg
    19681 agatatgtca atcagtcaaa cgatttgtga ttttgccaca cttgcgatcg aagctggcgg
    19741 atggatggaa atggaccgct tatatttaca gaatcgtctt ttagggatgg taggagaaga
    19801 tgctctagaa agcattgaag taagaccagt taaggaagaa tctgtagact tattggatca
    19861 gcttgtcgct aaggcaagag aaaatggtgt tctttcagaa ttgccagcag aacaagagat
    19921 ttttgaagcg caattaatgg attttctcac accgccacct tcagtcgtca atgcattctt
    19981 tgcacagcac tattcgaaaa gcccagagga agcaactgag tatttcttta atttaagtaa
    20041 gaaaaatgac tacatcaaaa caagagccat cgcaaaaaac atcaaatttc cggcagagtc
    20101 ggagtatggt gaactggaaa tcacaatcaa tttatctaag ccagaaaaag atccaaaaca
    20161 aatcgcagca gaacgcgagg cggtaaaagt agactatcct acctgcatgc tttgcatgga
    20221 aaacgaagga tatagaggtc gcttgaacta tccagcccgc accaatcacc ggattattcg
    20281 aatgaatcta gacggtgaaa gctggggatt ccaatattct ccttatgctt attacaatga
    20341 acattcgatc attttatcag aacaacaccg cccgatgaaa atcacaaagg aaacatttgc
    20401 tagactgctt cggattactg aagtcttgcc tcattacttt gttggttcga atgctgactt
    20461 gccgattgtt ggaggatcga ttctttctca tgatcattat caggcaggac gtcatgaatt
    20521 tcctatggca aaagcaccga tagaaaaact tatcaaacta gcagattatc cgcaagtcat
    20581 cgcaggagtc gtgaaatggc cgatgtctgt gatccgtttg caatcaacag agaaaaaatt
    20641 gttgattgaa gcagctaact cagtattgga aaagtggaaa gcatattcgg atgaaacagt
    20701 atctatcaga gctttttcag cagatggtac accacaccat acgattacgc caattgcacg
    20761 aagaagggac aattactttg agttggacct tgtccttcga gacaacaatg tttcagaaga
    20821 acatcctgat gggatttttc atccacataa agatgtccaa catatcaaaa aggagaacat
    20881 tgggttgatc gaagtaatgg ggcttgctgt tttaccacct cgtttgaaac cagaactagt
    20941 agaagtagag catttcttat taggagaaga aaaccagatg gctgaatatc atcgtccgtg
    21001 ggcagaacaa ttaaaaaaag aacatcctga tttgacgaaa gatacggtca ccgatgtcgt
    21061 acaaaaatct gtaggtcagg tttttgcacg tgtactggaa gacgctggag tattcaagcg
    21121 agatgaaaaa ggacaggcag cgtttgaacg atttactgca attttataaa cgtaaaaaag
    21181 gaggagagac aaatggcaac aattaaagac attgcgcagt tggcaggagt ttctcctgct
    21241 actgtttcaa gagttctcaa ctatgatcct gatctttccg ttggcttagc cacaaagcaa
    21301 aaaatttttg aagcagcaga acgcttaaat tatacgaaac atcgtaaaaa tgcagcaaat
    21361 actaagcaaa cgcttcgcct gatccagtgg tacgacgata cggaagagct tgaagatctt
    21421 tattatttat ctattcgtct aggaatcgaa aaaaaggcag aagaagcaaa cgttacttta
    21481 ttgaaagaaa cgtgggattc cattagtgat cttccgacag atggaacgat cgcactagga
    21541 aaattcgacg aagctcagtt attagaaatg aaaggatccc aaaatgcctt gcttttggta
    21601 gattctgacg gcacttctca aggaatcgac tctctagttg tcgactttaa gtcaagtgtt
    21661 caaaaagtca ttgaacattt tgtctcccat aaagcgcaac atatcgctat gcttgctgga
    21721 acagaatata cgaagaaaaa ccatcagcgg ctaaaagatc ccagaattat ggcatttcaa
    21781 caagttttgg cagaaaaagg ctacgacgaa ggtccaatta tcgaagcaga tttttctgtc
    21841 gagtcaggat atcaagcggt gaagcagtat ttaaaaacga atccgcatgt tcctgatgcg
    21901 ctttttgcag ctaatgatgc attagcgatt ggcgcactga aagcgattca agaaaaaggt
    21961 ttagtggtac cagaagatat ttctgtgatg ggtttcaatg atgtcagtgt agcgaaatat
    22021 gtaacgccct ctctttctac tgtaaaagtt tataccgaat ggatgggaga gctagcagtg
    22081 gagacgattc ttcaattaag taattccaaa gcaccggttt caagaaagat tattgtggaa
    22141 acagagttga ttattcgaga atcaacgaaa taaaagatgg aaaagctgtg gggaaatgaa
    22201 gtgataccgc agcttttttg tgtttcagga aggggcaaa
//
LOCUS       DACDYI010000057        21877 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.057, whole genome shotgun sequence.
ACCESSION   DACDYI010000057 DACDYI010000000
VERSION     DACDYI010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.057"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IWM52_002005"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IWM52_002005"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP9974251.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IWM52_002006"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IWM52_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAP9974252.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IWM52_002007"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IWM52_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAP9974253.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IWM52_002008"
     CDS             complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IWM52_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAP9974254.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IWM52_002009"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IWM52_002009"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAP9974255.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IWM52_002010"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IWM52_002010"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAP9974256.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="IWM52_002011"
     CDS             complement(8053..8523)
                     /locus_tag="IWM52_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAP9974257.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="IWM52_002012"
     CDS             complement(8569..8994)
                     /locus_tag="IWM52_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAP9974258.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="IWM52_002013"
     CDS             complement(9061..9330)
                     /locus_tag="IWM52_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAP9974259.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="IWM52_002014"
     CDS             complement(9341..10936)
                     /locus_tag="IWM52_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9974260.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IWM52_002015"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IWM52_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAP9974261.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="IWM52_002016"
     CDS             complement(14489..15049)
                     /locus_tag="IWM52_002016"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP9974262.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="IWM52_002017"
     CDS             15402..16376
                     /locus_tag="IWM52_002017"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP9974263.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="IWM52_002018"
     CDS             16546..17946
                     /locus_tag="IWM52_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAP9974264.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVNLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="IWM52_002019"
     CDS             complement(18024..18746)
                     /locus_tag="IWM52_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9974265.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="IWM52_002020"
     CDS             complement(18825..19331)
                     /locus_tag="IWM52_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP9974266.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="IWM52_002021"
     CDS             19454..21253
                     /locus_tag="IWM52_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAP9974267.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..>21877)
                     /locus_tag="IWM52_002022"
     CDS             complement(21321..>21877)
                     /locus_tag="IWM52_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAP9974268.1"
                     /translation="SSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTP
                     APTPTPTPEQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTR
                     YVYMQVTGRDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIH
                     APQPGESVKVGSVQWFAPDFAVSM"
BASE COUNT         6058 a         4795 c         3973 g         7051 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaataa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtca atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggtcgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagatt
//
LOCUS       DACDYI010000058        21031 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.058, whole genome shotgun sequence.
ACCESSION   DACDYI010000058 DACDYI010000000
VERSION     DACDYI010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.058"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..529
                     /locus_tag="IWM52_002023"
     CDS             <1..529
                     /locus_tag="IWM52_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9974269.1"
                     /translation="GYELDATPIEFEIERSQTAVVELTKANRLTPGGVVLTKIDDQSG
                     EVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPV
                     TFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGV
                     LLVFISIKFRKKRNI"
     gene            complement(953..1123)
                     /locus_tag="IWM52_002024"
     CDS             complement(953..1123)
                     /locus_tag="IWM52_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9974270.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1317..1496)
                     /locus_tag="IWM52_002025"
     CDS             complement(1317..1496)
                     /locus_tag="IWM52_002025"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974271.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            1883..2995
                     /locus_tag="IWM52_002026"
     CDS             1883..2995
                     /locus_tag="IWM52_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9974272.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3073..3366)
                     /locus_tag="IWM52_002027"
     CDS             complement(3073..3366)
                     /locus_tag="IWM52_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAP9974273.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3534..3848
                     /locus_tag="IWM52_002028"
     CDS             3534..3848
                     /locus_tag="IWM52_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAP9974274.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            3869..5848
                     /locus_tag="IWM52_002029"
     CDS             3869..5848
                     /locus_tag="IWM52_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9974275.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            5861..6187
                     /locus_tag="IWM52_002030"
     CDS             5861..6187
                     /locus_tag="IWM52_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAP9974276.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6203..6721
                     /locus_tag="IWM52_002031"
     CDS             6203..6721
                     /locus_tag="IWM52_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974277.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            6721..8074
                     /gene="celB"
                     /locus_tag="IWM52_002032"
                     /pseudo
     CDS             6721..8074
                     /gene="celB"
                     /locus_tag="IWM52_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
     gene            complement(8158..9663)
                     /locus_tag="IWM52_002033"
     CDS             complement(8158..9663)
                     /locus_tag="IWM52_002033"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAP9974278.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(9755..10345)
                     /locus_tag="IWM52_002034"
     CDS             complement(9755..10345)
                     /locus_tag="IWM52_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9974279.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10524..12170
                     /locus_tag="IWM52_002035"
     CDS             10524..12170
                     /locus_tag="IWM52_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9974280.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12219..12938
                     /locus_tag="IWM52_002036"
     CDS             12219..12938
                     /locus_tag="IWM52_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP9974281.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13417..14364
                     /locus_tag="IWM52_002037"
     CDS             13417..14364
                     /locus_tag="IWM52_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9974282.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14376..15296
                     /locus_tag="IWM52_002038"
     CDS             14376..15296
                     /locus_tag="IWM52_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9974283.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15337..16794
                     /locus_tag="IWM52_002039"
     CDS             15337..16794
                     /locus_tag="IWM52_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9974284.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17139..17753
                     /locus_tag="IWM52_002040"
     CDS             17139..17753
                     /locus_tag="IWM52_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9974285.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            17746..19470
                     /locus_tag="IWM52_002041"
     CDS             17746..19470
                     /locus_tag="IWM52_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9974286.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19467..20918
                     /locus_tag="IWM52_002042"
     CDS             19467..20918
                     /locus_tag="IWM52_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9974287.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         6781 a         3553 c         4235 g         6462 t
ORIGIN      
        1 ggggtatgaa ttagatgcga caccgatcga gtttgagatc gaaagaagtc agacagcggt
       61 ggtagaattg acgaaagcaa accgacttac accaggcggt gtcgtgttga caaagatcga
      121 tgaccaaagc ggggaagtcc tgcaaggagc tgtttttgag ttacaagatg ccaatggaac
      181 cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgatag atggcttagc
      241 accaggagca tatcagctgg tagaaactca agcgccaacg gggtatgaat tggatgcgac
      301 accagtaact ttcaagatcg aaaaagaaca agaagtggcg atctttttaa caaaagagaa
      361 tagaaaagtt gctgatagct cagatataag aggaaacaat ccaacaagca gtggaaataa
      421 gcatcttcca aaaactggag aaaccatcat tagccagttt atattgtcaa tattgggtgt
      481 gctacttgtt ttcatcagta taaaatttag aaagaaacga aatatatgag ctaaaataaa
      541 aaattgaata agcatagttt ttaaaatcaa ctagtaccca tcaattgaat ggaaactacc
      601 gattttagaa ggggtataca aacaaatatt agtatcggag tagagaaggt tgaacccaac
      661 cagtaaataa gtaaagacaa ttttgtgctg cgttgagaag tgaaaaataa acctttgtag
      721 taatactagt taataaaaaa tatacaaaaa cactgcgaaa atcattcttg agtttcgcag
      781 tgttttttgc ctaatgactc aagtttattg ggtaatactt tgattttctt tctacaaata
      841 atcagcgatc agttgtaatt ttttctctag ttttaatggc tctttgtcaa taaggagaaa
      901 aacaagtcgg gtcttttgtc tcggaaagct tcaaccaact cgtgaataaa ggttacgcct
      961 ccttgaaagg cgtggaaaaa ccaagtaatc gatcaatcac caatttcgca tttggtagaa
     1021 catgtttggt gagctgaaag taggtggcgt tcatgtctgt aactaagaat tctatttctt
     1081 taggattagt gcagctaagg aaatagtttg ttaatcgagg aattttttgt gtaggtaaat
     1141 tttctcgatt aatttttctg tctcgccatc cgcggaaata aaactcagtt tatcctcaac
     1201 ggaaacgtga gaacgaaatt cacaaccctc aacactcaag acagaattct tttggaagac
     1261 tttggtaaat agcttctcaa ctctttcaat gtgcgaataa cggttgtcaa agatacctaa
     1321 caacttttcg aaataaaaaa taacgacact ttttcagtca gtaaagaagt gattttatgt
     1381 ctgacatgat ttgaaatcga gtgccttgga cggataaaat aactttgagc gttccaatgg
     1441 cttcgacagt ttttacaggt atagcgctgc ttttttaggc gtataaccat tggcatatga
     1501 ttatattatt caaagtggac aatggtttcc ttcttcttgt ttttcaccac cattaatttc
     1561 ctatttccat ctaccacagc agaaccacaa tttctacaga cacatggagc agggaagaga
     1621 actgcagcaa cgatcaatgt ttttttcttc tgtaaggttt cgtaagagac ctctgtaatc
     1681 atcaaatttt tgtctattga tctcagaatt tttttgatag aaccattcat aaagcatatc
     1741 gttctctcag ttgtttattt tgtggtgatt taatcataca agaaaacgat atgttttttt
     1801 ataaaaacat gaataggaaa aatagttgac aagaaaatga caacgccata tgatgttcca
     1861 tagtatttac aacctgtcat agatgactgt aaaaaataaa agtaaaaaga aaggacgaca
     1921 acaggtggat ttttatcagt ctctacaact agatccgttt attttaaaac aaaaaatacg
     1981 agaagcagct tctaaaaaag aaaagtgcat gtatatttgt tcattgtttt tacgatcatt
     2041 attcatcgta ttgtttgcca tttgttttat cattataatt acaacattat ttgaatcgaa
     2101 gcataaacca tacgctgtgg ttttattctg tatgttgatg agtattcgtt ttgttgactt
     2161 tggttataag atttcccatt cgatcatagg tttagcaatc gtcatgttct cactattagt
     2221 agcaccgtat gtccagctga tcaaatggtc agttatgggc atgttgattc attttatttt
     2281 actaagcagc atattaatgg caaccgctag tgatcccaaa atggggaatg cttctttgta
     2341 cggcttttca tatctcttta ttgtttattc gttacctaaa gattcgttga acaagaattt
     2401 ctttacacaa acaagtagct tattattttt gtttttttgt tggttttcag ttctccttta
     2461 cagaaaacat cgagaaaaga acaaagacaa aagcttgttt aaagagattt ttttgaaagg
     2521 catgtattcg caggaaaaaa tttggatgct catctatgca tttggcatta gtcttttgat
     2581 cgttgcagga gagtatgtcc cttttcagcg attgatgtgg gcagggtttg cattttcttc
     2641 tattgtatct agctatggat tgatgtcgat tggtttcaaa gaacgagcag tagaccgaat
     2701 cattggctca ttgattggat gtgttttatt tattggtatc agccaattta ttccttttaa
     2761 ctgggtcggt atattaggtg gtctagcttt aggtgtttgt tctacctata gatataaaac
     2821 agtttttaat tcttttggtg ctttggcaat cactgccagt ttattcgggg ttccaggagc
     2881 agtaacgatc cgcatttttg agaatatcct tggggtatgt ttaggaatca tgtatattgg
     2941 ggtgactgaa atacttatcc gaaaaataag agaaaaacac ggtttgaatc attagatgag
     3001 atcgaataaa aaataaggaa aaagtgaaga tacacatgga tgataaattg ctatcattcc
     3061 atgtgtatct ttttacggta aatcagtgaa acgttcgact ttcattttta atttatactt
     3121 tttacggagt tcgtttattt gagccatcat ttcggatttg tgatgctttt cgattgcttc
     3181 ttcatctttc caacggtcga tcagtaaaag agattctgga tcttctagtg gaaagtaata
     3241 gtcatatttc tcgtttcctt cttctgcacg aatcgctttg acaatgtttc tttcgaccat
     3301 ttcttctgca aattttctgg cttgtccatt tgtacttgta tacaaaatat tcactgtgat
     3361 actcactaaa tcccctcctg aaaaaattct atcactagta tatcacggtg ccagaaatta
     3421 ttattcaaga tatgatacac attttttccg caatgctacg gaaatatata ttttgaatag
     3481 aaaacgctta caagatatgc ttggtttata aaaataaaag gagtgattga tttatgaaaa
     3541 aagcattgat tatttgtgcg gcaggaatgt catcctcgat gatggcctca aagacaacgg
     3601 atttctttaa gcaaaaaaga gaagaggtac tcgtagatgc agtttcagct acagaaggcg
     3661 ataatatgat taagaatagt gattttgatt tatttctgat cagcccgcaa acgacgatgt
     3721 ttttggataa attcaaaaaa ttaggagaat ctgttggaaa gcctgttgtt agtatccctt
     3781 tccaagcgta tgtcccaatc cctacaggca tccagaagtt agcagaggtg atcgaagaga
     3841 acatatgatg gaataggagt ggatgataat gaaacagaag gaaaaattgc tgttgagata
     3901 tttgattgat cacaagaagg agtatgtcac cagtcaacgt ttagcttctg aattgttatt
     3961 atctgatcgg acaatccgta attacttgca gcggatcaaa gagttagtag aaaaaaacgg
     4021 aggtaagatc attgctaaac caggttatgg ctaccaactg catattttac aaagactgac
     4081 atttgacttg tttttgagtc ggcaagaaat agtacctgga tatgctagag aaccgcaagg
     4141 attttatgag tccgaagatc gacaaaagta tatattgaac caattgcttt tggaagatgc
     4201 cattcttttt atggatgatt tggcagaaga gctttatatc agtcgttcca gtttgacaaa
     4261 ggacatgcaa gaaatcaagg agcgattgat tccatattca ctaaaaatcg tttctaagca
     4321 tggtcaaggg acatggatcg aaggggaaga aagaaatcga cggcatttta tcatggatac
     4381 tttttttgga aaccattatg gaaattcatt gaaagaatat ttagggcaaa gccgtttttt
     4441 cccagacatc agttttgaag agctagtcat tatcattctc gatgaaacaa gagaagctaa
     4501 gttaaaagtt tcggatttta tcatacagaa tctgacttta cacttggctt tggggatcaa
     4561 acggctccga gaagggtttg aaatcaagga attaggcatc gaacaagaag tttctaagcg
     4621 agtagagtat caagtggcgc agcggatcgt tcaacgtatt gaggtaatcg caggtgtatc
     4681 gtttcccgaa gaagaaattg cttatttgac attacatctc atggctaagt cgaatcacaa
     4741 agaaaatcaa aatgatcagg aactatcaaa ggaattagcg aaagttctga aaagattgtc
     4801 tcaggaattt catttttcat tagtagagga tctccagctt ttcaatggac tactcgatca
     4861 tttgtcaccg atgatgattc gattgagccg aggtattcgc attgagaacc cattgactga
     4921 ggagataaag aaagaaaatc ccaaagtctt tgacgcagta aaaaggcatt tcagcaacat
     4981 gccggctttg aaaaactata ccatcaatga agatgaatgg gcctatttgg ctttgcatct
     5041 catggcggca ttggaaaaag aaagagctgc gcataaacta catgcattga ttatctgtgc
     5101 aacgggttac ggcagtgccc aactattaaa gaatcgcgta gtcagtgaat tcggtaaaaa
     5161 tattacagtg gtaagtgtaa aaggctatta tgagatcaat gaaacaactt taaaaggaat
     5221 cgacctgatt atttcttcta ttgatctttc gacaatgttt ttcaaaattc cagttttaca
     5281 tgtcagtatt tttttgaatg aagatgatgt tcgtaaaata cgaaaggtag ttggagaatc
     5341 tataccctat cattcctcag atagaccgat accagcattt tctctacagc aaaaaaagca
     5401 gatttacacc gaacagttgt ctgaaaaatt tttcaagact tatacgtacg aaccgaaaaa
     5461 ggaagaaatt cttcatgatt tgctgcaaaa gctgagcata aatgaagaag aaggatatat
     5521 aagagaaatg cgaaagcaga tccagcaacg tgaaaatatg ggacaaatca tattcagtga
     5581 tacagtggca gtcccacatc ccgctgttcc agttggaatc gaaacaaagg tagccgttgc
     5641 attgattcct tctgggatgc agtgggatca atatcaagaa attcattttg tttttttagt
     5701 ttcgccttct tatgtcgaaa atgaaggaat tacggtcgtg actaaagcaa tcgttaggtt
     5761 agttgaccag ctaaaagtac agcaggaaat cttagctgaa cctatttttg agaatttcag
     5821 cagtaaattt atcaaactga tcgaataagg aggaaaacag atggctgaaa aaataaacag
     5881 tgaaacacta caagtgactt cttttgatat cattttccat agcggaaatg cacggacact
     5941 gatacatgaa gcatttcgct cgatgcgtaa agagatgttt gaagaagtag aagagaagtt
     6001 gcgacaagca aatgaagaga ttcttcatgc gcatcagtca caaactcaac ttctgaaaga
     6061 atatgccagc gggacaaaaa tagaaatgga aattatcatg gtccatgcac aagatcatct
     6121 gatgacgacg atgacattat tggaagttgc acaggaaatg aattatttgt atcaaaaaaa
     6181 taaatagaaa ggaaggatca ttgtgcagga tacagtaaaa aggaaaatcg aattagaaaa
     6241 aaagcaactt agtttgaaaa acatgtattt caatcgttat ctaatcgtac gttacctgac
     6301 tgcctttttc tttttcatga acatgcaatg gatgattctg cttctatcag ctaaaagttt
     6361 aggaagtagt ttaccgatga ttctgatttt agctattttg cctgcagtag gagaacaagt
     6421 aaagctttat cgaaaacacc aaacgaacgt tccatggaca aagtggtatt ttttgttcca
     6481 gagtgtcttt aatttattag tgattccagt gttgtttacc tcaggattta cattcttgta
     6541 tccttttatg gcaaataaca atggaggaca gttatttgtt ttcattcttg ttgtaagcgg
     6601 tatttttgcc agtgcattta tacagtatcg tttaaagaaa atttcactca accaagacca
     6661 acagtatatc cgtattaaac aatatgaaaa agcgttatat ttaggaaagg agaataacta
     6721 atgtttgatt ttttgcagaa atacctgatg ggacctatgg ggaaaattgc gcaacttaaa
     6781 atcgttcggg ctgtcatggc agcggggatg gcttctattc ctttcacgat tgtaggctcg
     6841 atgtttttgg ttttaaatat tttgccatta ccgttcccat ttttagaagg attttttaat
     6901 gcaacttttt tcaaagttag tgacttgtac atgatcgtca atacaatgac gatggggatt
     6961 ttatctattt actttgccat tgtgtttgct tatgaattaa cgtcaattga acgagatgaa
     7021 caaaaattaa atgttaatcc gctcacgggg gcattactat ctgtttttgc attctttatg
     7081 tgtatccctg aattgatcat gactgatggt aaaatcagct tgattgcttc tatcaatgag
     7141 acagaaactt gtagtaaatg gtattaggat gggctctttt gtcgaacgtt taggaacttc
     7201 aggaatcttt acagctatta ttatgtcgat tattgcggtc gaattgtatt gtatgtgtgt
     7261 caagagaaat tgggtcataa aaatgccaga tgttgttccg ccaggagttt ctcgttcttt
     7321 cactgctctg atcccaacat ttgtcattgc gtttgtggtg atgatcatca atggattatt
     7381 agttgcacta ggaacggata tcttcaaaat gattgctatc ccatttgggt ttgttacgca
     7441 gttgacgaat acatggcttg gtgtcatggt gatttatttc ttgatccatg cattgtggat
     7501 cgtagggatc catggagcga atatcatcac ttctttcttg accccgatcg tattagctaa
     7561 tatggcagct aacgcacaag gcgcaaatta tccgttggca ggagaattca acaattctta
     7621 tgtaaccgtt ggtggatcag gtgcgacttt aggattgatc atctttattg cattcctcgc
     7681 gaaatctgat cagctgaaag tgttaggaaa agcctcactt gtaccaggat tattcaatat
     7741 caatgaacca atcattttcg ggatgccaat cgtctacaat ccctatttag cggttccgtt
     7801 tttcttagct ccgatggctt ctgctacact cgcttatttt gccattcggc tggaaatcgt
     7861 caaaccgatg cttgcacaga tgccgtggcc ttcaccagtc gggatcggag catttgttgg
     7921 tagtggtggt gattggaaag ctgctgtgtt agcagtcatt tgtgcaatca tcgcattcat
     7981 tatctggttc ccatttatca aattctatga taataaatta ttaaatgaag aaagagaaaa
     8041 ggcagcagaa ttagctagtg aaaagttaaa atgatttcta agaaaaaagg aactgtgaca
     8101 aaaaatcaca gtcccttttt ttcttagaaa tgggaaacca ctagtatata aataccatta
     8161 ttcttcaatc aaccgcttca cgattgcata atccccttca taaggttcat ctacaccatt
     8221 gattttttga tagggatcca cgccttttcc cgcaataacg actgaatctt gcggagcgct
     8281 ttctgctaaa gcgcgtcgaa tcgcttcagg acgatccatt tcataatgaa cgatcaaatc
     8341 tggattagta attgcttcat tgatttcttc cgcgattttt ttcggatctt caaatgctgg
     8401 gtcatctgcc gtcaaaaagg cgatgtcagc tgtttctgat agcacttttc caaaatcatg
     8461 tcgacgggag attgctttgt ttcctgggct gcctaataca acaattacac ggccatctgg
     8521 atgttcattt ttggcaaatt tcagcaatgt tttcaagctt agataattat gtgcatagtc
     8581 cacatagaca tgcgctccat tggattggat caattgatcc attcggcctg gtacacgagc
     8641 ttcttttaat ccgtcttgtg catcttgact ggaggcacct gttagtgctg cggcaatgat
     8701 cgcactggct gcattttctt ggttgaatcc tccagccagc aaaatatcat aagcagtatc
     8761 gcagatagct aagcgatctt catgagaaga taaagtaaaa ccgtgagtac ctttatctga
     8821 acggatcact tgataattgc tttcttccga acgaccataa gtgatcgttg gaatctggaa
     8881 tagatcggct gtttccctaa gtaaatcaaa atagtcactt tcatgattta aaacaactgt
     8941 ttttgaattc aagatcaatt gtcgcttgca atagaaatag tcatcaaatg tcggatgttc
     9001 gatcggactg atatggtcag gcgagatatt caagaagatt ccgacatcta aagtcaagcc
     9061 gtatacgcgt tgtgttttgt aagcttgtga agaaacttcc atcactagat gagtcatacc
     9121 attttcaact gcttctgaca tcatacgata taaatctaat gactcaggcg ttgtcaaatg
     9181 ggatttgaaa tatgttttgc catctaacgt tgtattcata gtagaaagca atgctgtttt
     9241 tttatttgtt gtgtgatcca agattgcttt tgtaaagtac gcagcagttg ttttaccttt
     9301 tgttccagtg aatccgatca ctttcaattt attttgcgga tagtcgtaaa aagccatagc
     9361 aagaagcgcc attgctttac ggatatccgt tacgatgatt cctactgctg aattttcgta
     9421 tggttgttct gatacataat attgaatacc ctgttcgatt gctgaatcca aatattcttc
     9481 tttgaaattc attcctttac agaaaaacaa agtagaagca tcagcttttc ttgaatcata
     9541 acttaactga agaaattcag ctttttctgg tacatcaaga tgccagcctc ttgttgaaac
     9601 aaattctttt aatagatttt cttttaatag taaatgacgg atgtcttcta aagagaagct
     9661 cattgattga tccctcgctt tattcaattt cttggtttat tatagcacac tctttttaat
     9721 atatcgtttg tttccgctat gacaaaatcc agctttattc aaattttctt gacacagggg
     9781 acaagcggat gagtattttc ataaacaatg aactgactgt aatagatagg agaacgaata
     9841 gtgtctccaa caatccaaaa acataataat tcaaaaagac gataagacta ttggtaagta
     9901 aaagtccttg cggcattgga agccgaaaaa atttgtggaa aataatcggc ggtatcgtta
     9961 taccgccatt tgaggccccg actgtgtaca atgtgtaaac cccaagactg aacaaaagac
    10021 ttcctgaaat cagtgaaaag aaatgtgagg agatcatttc cacttttgga acaatctcta
    10081 aaacaacagg aaaaagcaga cttcccgcta cagtcttaaa aaatactgat tttcctaata
    10141 ggacagcggc tagaagcagc atcataagat tgatgctcca aacagtaact gagcgttgga
    10201 tagaaaaggc cgtttcaact aaatgtccga tggcgcccac tccggcggaa gctatatcgt
    10261 gagggagcaa gaaccaattc aatccaactg tgataaagaa aagacctaaa aatataacgg
    10321 gtagtttttt tatttcttgt gtcattgata tctcctataa atgttagcca taaaaggctg
    10381 tatatttctt tactttacca aaaaaacgaa accgaattca aaaaaacaat tgttcttata
    10441 aaagttatat aaaatcgatt gccagtttct gttttaccaa ttaaaatgat agtaatgttt
    10501 tgtagaaagt tggaatctga ccgatggata atcaaagaaa accagcatta gatcaattaa
    10561 caccgcaaga aaaaatggca cgaggctctg cttggatgac cgccagtaat atcatttcaa
    10621 gattgctagg cgctgtctat atcattccct ggtatgcttg gatgggcgaa aatgctaagg
    10681 ctgccaatgg attattcaat atgggataca atatatatgc attgtttcta ctgatctcta
    10741 cagcaggaat acctgctgcg attgcaaaac aaactgcacg ctataattca ttgaatgaat
    10801 atggcacttc acgacgatta tttatccgtg cattacagat gatgggcggg ttaggcctgt
    10861 tgtttgcttt gttcatgtac atcgcctctc cttggctagc tcatgcttct ggtggcggag
    10921 aagaactgat tccaacgatg cgttctttga gtatcgctgt attggttttt ccttgtatga
    10981 gtgtgatacg agggtatttg caaggcaatc aagaaatgat gccttatgcg ctgtcgcaga
    11041 ttgtagaaca ggtggcacgt attttttata tgctattggc tacattcatc atcatgaaag
    11101 tcatggatgg tgactatgtg acggcggtca cgcaatcaac tttcgcggca tttatcggta
    11161 tgctagcaag tattcttgtc cttttgtatt ttttgaagaa acatcaagcg tatacatcgg
    11221 cgtttattca ctacagcgaa aacaaaataa atattgcaac gaaggaattg ttgttagata
    11281 ccgttaaaga agcgattccg tttattattg tgggttcagg ggtaacgatc ttcaaattag
    11341 tcgatcagtt cacttttatg aagatcatgt ctgcatccac cgagtattcc aatgcacagt
    11401 tactggatct tttttcgatt tttagtgcca atccagataa gttgacgatg gtagttattg
    11461 cattggcaac atcgatcgcg tctactgggt taccgttgat tacagaagct gttacgttga
    11521 aagatcgagt aggtctggca aaattgacaa gcggaaattt acagttgttt tcatttttta
    11581 tgtttccggc gacatttggt gttatgttac ttgcctatcc gctaaataca ttattttata
    11641 caccggattc tctaggaagt aatgtactga tccaagcaag ttttgtagga ctcttcctag
    11701 gactatatat gctagtttcc aacatgctgc aaggaatgtt tgaaaacaag gcggcaatta
    11761 attatttggt tattgggttt ctagtgaaac tccttcttca atacccagcc attcgtttat
    11821 ttgaagtata cggcccttta ttggcgacaa tgatcggatt tgctgtgtct tgtaccttga
    11881 tcttaaagag aatccatgaa gtagcccgct ttaaaccaag ttttgtctgg cgcaggacat
    11941 tgctgatctt tattcttacc ttgattatgc ttttggcttc gtggatcaca aagatgattt
    12001 ttggtgcttt tttaaacgaa gatagcaaat tccagtctct attgttgatt cttgtagtag
    12061 caggtatagg ggctttagtg tatggttatt tagctttgaa aatccgcttg gcagaaagac
    12121 tactaggacc aggaatagcg cgtttacgac ataaattaaa aataaaataa gtgaatcaga
    12181 agagctagat tgatgctctt ttgaaatggg ggaaacgcat gcgtttagat aagtttttag
    12241 cagatatggg acttggcagc cgcaaagaag tcaaaggatt aatcaaaaaa ggaacgatcg
    12301 aagtcaatgg acaagtggtg aaaagcgata aagcgcaagt aaatgaattc gaagatgcgg
    12361 ttacttattt aggagaaatc attacgtatc aaaaagattt ttactacatt ttgcataaac
    12421 ctgcaggagt catttctgct actcaggata atcatgatca aaccgtaatc gatttattgg
    12481 aagaccagga ttatcgggaa gatctttttc cagtcggccg attagataaa gacacagagg
    12541 gcctcttgat tttaacgaat gacggggtgt ttgcccatca gttactatca cctaaaaaac
    12601 atgtggagaa agaatatcta gctgaggtta gcggtataat gacagaagaa gatatccgtt
    12661 tatttgcaga aggactgatt attgatggcg atgagcagac attgccggca aaactccaaa
    12721 ttgatcaagt agatcaagag acagaaactt caattatccg tctgattttg catgaaggaa
    12781 agtttcacca agttaagcga atggtcaaag cagtaggaaa agaagtctta tatttaaaaa
    12841 gaatccgtat gggaaaattt tggctgccta aagatttgaa taaagggcaa tatcgaccca
    12901 tgaccaaaga agagctagaa caagtgagag aaaaataatt gaatggatag ctaaaagaat
    12961 aagcagagta gttgtagcgc catctgaaaa gcagacttgt ccgtctgact ttcggatggc
    13021 ttttttgtga aatttcggct tatttccgta gaagctactt ctggaacacc gtttgttcgg
    13081 aataagggac tgtgacaaga cttttgttac agtccccatt gttccttgtg actcagagtt
    13141 agatagtaag gacacgatct ccctaatact caggccaaaa cgctgaattc acaacctctg
    13201 ataaacggtg ttcaatcagc tgaccctcgg tattagctca aaaaacggca aaatatgagg
    13261 aactattttc ccgttttttt ctctaatact caggccaaaa cgctgatttc acaacctctc
    13321 ctatactttg tttcattttc cctatacttt gtttatttat tctcattatt gaaagcgcta
    13381 taattacgtt gcgaatgaat cgaaaggggt ttttagatga aaaaacgaaa ggggttcaat
    13441 cattttttta tgaatctatg gcgttataaa gcacttgtcc tgatggctgt accgggtatg
    13501 ctttggatga tttttttctt ttacatacct gtaatggcga atgtcgtagc attcaaaaat
    13561 tttcatattt cagcagatgg gttcttggct agcttgaggg aaagtccttg ggtgggttgg
    13621 gaaaatttca aatttttatt ttcttcggat caagcatttc tcatcactaa aaatacaatt
    13681 ttatataatg tagcatttat ccttttgaat ctgctgatct cagtcgtatt tgcaatcatt
    13741 atgagcgagt tgagaaataa acgactcgtt aaagtttatc aaacgatgtc cttactacct
    13801 tattttctat cttgggtcat tatcagttac tttgtttacg cttttttaag tcctgacaaa
    13861 ggaatattca atcagtggat cactggacat ggaggagaag ctgtcaattg gtataatgaa
    13921 ccgaaatatt ggccgtttat cttggtgttc attggtacat ggaaagggat tggttataac
    13981 agcattattt actttgcatc tgttatggga atcgatccga cttattatga agctgctatg
    14041 gttgatggag ctagcaaatg gcagcagatc aaacatgtga cgataccaca acttattcca
    14101 ctaatgacga ttttaacgat tttagccgta ggaaacattt tccgagctga ttttggcctg
    14161 ttttataaca ttcctaggaa ttccggtgca ttatatgatg tgactcaagt tttggatacg
    14221 tatatctaca atggcctgac atccacagga gattttggta tgacggctgc tgcaggactt
    14281 taccaatcag ttgtcggctt tgtcttgttg atgatcacaa atacgatcgc ccgacgtttt
    14341 gatagcgatt ctgcattatt ctaaggagga aaaaaatgaa aaaaaagaaa gtggagagag
    14401 tccaagtccg ctctttcaat aagacggcta attttttctc taatataatg attgcactct
    14461 ttgctatttc ctgtattttg ccatttatat ttgttatcgc catctctttt accgatgagt
    14521 ccatattgtc gactcacggg tacagttttt ggccagaaaa gttcagtata tttggttata
    14581 cgtacttgtt cgaccagatg caggataaaa ttttccaagc gcttttcgtc actgtacttg
    14641 ttactgtttt aggtacactg atcaatagca cggctacttc tttatatgct tatgcgattt
    14701 cacggtcaaa ctttccattc cgtcgttttt ttaccgtttt ttgtttgatt acgatgttgt
    14761 tctcaccagg gatggtggcc aattatcttg ttatgacaaa tcttcttcag ttaaaagata
    14821 cgatttgggc attgatcttg ccaatggctg tcagtccctt caatattatt gtgatgcgca
    14881 cattctttaa acgttcggtc tctgattcga ttattgaatc ggcacggatc gatggagcta
    14941 gcgaactgcg aatatttgtt cagatcgttt taccgttagc tgttccaggt attgcgacaa
    15001 tcagtttatt tgccgcattg ggttactgga atgactggtt caatgcttta ctatacatcc
    15061 aagatgataa gctagttcca ctacagtatc ttttgatgaa aatccaaagt aatattcaat
    15121 atctgacaca aaattcagga gcaggaagcc aattgacagg cggattagcc tcagttccag
    15181 gagaatctgc cagaatggcg atcgtagtca tctcgacgtt gcctattgcg atcagctatc
    15241 catttttcca gaagcatttt gtcaaaggtc taacaatcgg cggagtaaaa gaataaagaa
    15301 acaaaaatat aaatcataaa attaggagga agaaaaatga agttatggaa gaaactagcc
    15361 tttacaggtg tttcagcgtt gttgttggga acattagcag catgcggagg ttctggttct
    15421 aaagaccagg cagaagctag caattcggac actttgcaga tgtatcaaat cggagataaa
    15481 ccagataatt tcgatcaatt gatggaagta gcaaacaaac gaatcgaaaa agagatcggt
    15541 gtaaaagtca atatcaatta cattggctgg ggcgattacg aaaagaaaat gaacgtcatc
    15601 atttcttccg gggaaaatta tgatatcgcc tttgcgaaca actatgtttc caatgcccaa
    15661 aaaggagcct ttgctgattt gactgaatta gcgcctaagt acgctgaaaa agcttataat
    15721 gatttagatg aagcatatat taaaggaaat ctggtcaatg gaaaattata tgctttccca
    15781 gtcaatggaa atgtctttgc tcaacaagta cttactttta acaaagcttt actagataaa
    15841 tacgatcttt caattgatgg aatccaatct tacgctgatg cagaaaaagt cttgaaggaa
    15901 ttccatgaaa aagaaccaaa cacggcagct tttgccattg gacaaggctt caaagtccaa
    15961 gggaactttg attatccttt agggaatacg ttaccatttg ctattgatct ggatggagat
    16021 gattcgaaaa tcatcaacca atacgataat gaaacgtata tcggactgtt acgtacaatg
    16081 catagctggt acaaacaagg actcattcca agtgacgctg caacgagcaa taaggattat
    16141 ccactagaag gaaatacttg gctaatgcgt ggagaaacac aaggaccgta tgattatggc
    16201 gatacaattt tgacaaatgc agcaaaacaa gaacttgtct caaaagcaat tactgttcca
    16261 ctaaaatcta cagctcaagc acaaatggca aattttgtcg tatcaaatac ttcaaaaaat
    16321 aaagaaaaat cagtagaact tctagggctg cttaacagtg atccagaatt attgaatggg
    16381 cttgtttggg ggatcgaggg agaagcatgg gaaaaagtcg atggttcaga tgataaaatc
    16441 aaattgctag atggttatca gccaaataca catatgtctg cttggaatac aggaaacaac
    16501 aagattctat atacacaaga gtctattacg gatgacatga ttgccaaacg tgatcaatcg
    16561 attgctgatg cagaaacttc accgatttta ggattcagct tcaacactga cagtgtcaaa
    16621 acagaattaa gtaatatttc taatgtcatg aatcaatatt tagatggatt gaatacaggg
    16681 actgttgatc cagatgaaac attaccgaaa ttgaaagacg ctttggacaa ggctggctat
    16741 gataaggtat tgaaagaaat gcaaaaacaa tatgatgagt tccgacaaga ataataatga
    16801 ataaagacaa aggggttgtg acaaaagacg tgtcacaacc tcttgttccg aataaacggt
    16861 gttcaaaagc cagtttctcg gtattaagct aaatatatgt aaaaatgtgg aaagccatat
    16921 tcacaaaatt tttcttacta ctcgaaacta agcggctatt tcacaaactc ttggtaaacg
    16981 gtgttcaatc agctaaccct cggtattcgc tcacaaaacg acaaaatatg aggaactatt
    17041 tttccgtttg tttctctaat aatcaggtca aggtgctgat tgcccaacct ttttttgtat
    17101 aatgagaaag tataaactta atgtttggag gagaagatat gttagggaag gttttggaag
    17161 agttatttat ccggatctgg gtcattataa aactaacctt gtatttctgg atatatacat
    17221 ttgccggcgg gatcatcttt ggattaggtg cggcatggaa aacagtcaat gaactatttt
    17281 atttgtacgg attcgagtat aaagaaatca caataaaaag aggctggaat atatataaaa
    17341 gaaatttttt aagaggcaat ctgctattta gtttattttt atctggtact gctttattga
    17401 gttacaattt atatttatct gttcaaatca aagggctcat ttttttagct attgatttta
    17461 ttttactttt tgccttgctc tgcctatttg ctacttatca atacagtatg attttagata
    17521 gtgaatatac gatctctgtt cctaatctgc taaagctaag ttttatttcg gttttttcca
    17581 gcttttcgtc ttttctaaaa ataattattg gtagcggtat aattttaggc gtgacttggc
    17641 aattcaaagg attgatcctg tttggtgtca ttggtctttt gacggtttgg aatggtacga
    17701 tgacaacaca ttggcgtgaa gaattagata aacagttgga aagttatgaa taagtttcgg
    17761 attctatttt caaaaggaac cttactaaat cgtctgttgc ggaaatacgc ggttatcatg
    17821 atcggtgtaa cgatgactgc taccgttatt tttagtatcc atacttggga acaaaatcaa
    17881 aaacaggcag aaaacatgac aagtgatgcc gttcaaagca caagcagaat gctgaatgat
    17941 aaaacaacct tgagtaggat catcaaaaac cagcttgtag ggaattccga aaagatcgaa
    18001 aatgtgacga cctacttaac aaaacccata gatcagtatc tgatgtatgt atacgaacag
    18061 caaaacagta ccgatgaatt agtttctttc ccaaatcaaa tcaaagatct ctacgcaaat
    18121 tatgaagaac taagtgcgat atatatcgtg ttgaatcaac taccggagta ttatgaatct
    18181 tctagagaaa acaaaggtgg ggaaataaaa aaggggacac caaaattact agacgccttt
    18241 tatatcaagt tgcctatcac acaaggagga gctgagtcag gaagtatttt tattggtttt
    18301 caaaagaaag aactagatat gattttagaa aatctaacgt cattcaatgg actctcttta
    18361 tacatgattt ctggaacgac aaatcgacta tacacattcc atgataataa aatagctaaa
    18421 gaaacgtttg aaagacaaga aatgatcatc acagagagtc tgaagaaaaa ttcacaattg
    18481 ccgattgacg atctggaaaa aaacaatttt gttcaacacc aagaactgtc tgatgattat
    18541 cgtatattag ctgtactaga taaaaaaagt gtaaaaatcg aaacgggaaa aaatcttgcc
    18601 ccgctgatca ttggtatcat tattttagac aatatactgc ttatctttct ttataaaaca
    18661 ttcaaacgat attcacagca agtatctatc gtcatgcagg ccatggatca aacagcagca
    18721 gggaatttag agacgcggat cgacataaca aacacggaat atgaactgaa ggaactttca
    18781 atcggtatca acgaaatgtt ggatagcatc aatcagtttg tagaagacat ctacaaatta
    18841 gaaatcaaac agcaagacgc acatatgcgg gcgcttcaag cacagatcaa tccacacttt
    18901 ttatataata cattagaata tatccgaatg tatgcaatca gcgaaggaag tgaagaatta
    18961 gcagatgttg tgtatgcgtt ttccgctttg cttcgaaaca atacgaatca agagaaaacg
    19021 atcactttaa aagaagaact ggacttttgc gaaaaatatg tctatttgta tcaaatgcgt
    19081 tatcctaatc gagtagccta tcattttatg atcgatcctg atttggaaaa gatcgaagta
    19141 cctaaatttg tgatccagcc attagttgaa aattatttta aacacggtat tgattttact
    19201 cgattcgata atgcgctaag tgtcaaagtc ttgcaggaag gcaaacgggt aaggatcatc
    19261 atcaaagata atggaaaagg gatgactgaa aaacggctaa aacagattga agaaaaactg
    19321 tctcatccta aagtggaact tcacggttct atcggtctgc aaaacgtgaa tgaacgttta
    19381 cgtgccagct tcggcagcag ttattatatg tcgcttgaga ataatgagac aggtggactg
    19441 accgtttcta ttacattcaa ggagggatga gcgtgtatca agtgttattg gtagatgatg
    19501 aatacatgat tttgaatggg ttaaaaaaga taattgattg gcaaagttta ggctttcaaa
    19561 tagtagcaac agcagagaat gccaaagaag gactagctgt acttgaacag cgacagattg
    19621 atttagtagt aacggatgtg acaatgccag aaataaatgg actggaattt attgaagcgg
    19681 ctcaaaaaga acgtcataat tttgaattta tgattttatc aggataccaa gaattcgatt
    19741 atttgaaagg aggtatgcaa ttaggcgccg tgaattattt gatgaaaccc gtgaataagt
    19801 tcgaattggt tgaaagcctc aagaaaatca aaacacggtt agatcagcaa aatgaacaaa
    19861 aaaatcaaca agaaatctat caagaactgt tattcagtca atggttgaat gaagaattgg
    19921 atgaaataag tgaagaagaa ataataggac aaataagtga gaagcagcat aggatagtat
    19981 tgattcaact ggcaagaact catgatactc ttatcaatag atggttgcaa gaacggcaac
    20041 aaatctttta ctatcaacga aattacggag accgtgtatt gatcacgttg ttgttggagg
    20101 cagatgaagt tgatggcttc tgttgttttg ttcaagaaaa ctttcctgat caggaatggc
    20161 tgatcagtat cggggaagag acagattctt tggacaaaat ccctgaaaca ttccaacaag
    20221 cgaaagacaa tctacagttg catcaatttt atggagataa agacgagcat gttttttacg
    20281 cagtacaagc agaaacaaaa gatcaaacca tcgatttttc aagtttcaga cgcgtgctgc
    20341 aaaataaaca attggaagtc gctcaaaaaa tgatcactga tttttttgaa cagtttcagt
    20401 tagcagccat gatgccggaa gatatacgat attccagttt tttactgttt atggagattc
    20461 aacgggaatt gattgatctg gaagatgagg aatatttaca aggaatcgaa aaaatccatc
    20521 aggcaaaaac ggtgcaagaa cttcagcagc tacttctgtc attcatcaaa aagcatcaga
    20581 gacagaaaaa gtacagtgac aatgtggaaa aagtaatcgt aatcctgcac cagcattatc
    20641 aagaaccact tacactcaaa gaagtgtcag aaaacctgca tttaaatgtc atgtatctag
    20701 gacagctgtt caaaaaagaa acgaaaaaaa gtttttcagc ttatttgaac catttacgta
    20761 tggaaaaagc gaaacaactg cttttgcaca gtaatcaaaa catcaatgag atagcaagtg
    20821 agattggcta caacaacacc acgtatttct ctaaattatt caagaaaatc gtcggcagat
    20881 cccctaaaga gtaccgagaa aacagtggag aatcataaaa gaaaaagcca atgtaatgaa
    20941 ataacttata aaaaattgtc tagaaatcta attttgggct gttcttatta cttgaggcaa
    21001 ggcagctttt tcatgacctc tcgataaacg g
//
LOCUS       DACDYI010000059        20664 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.059, whole genome shotgun sequence.
ACCESSION   DACDYI010000059 DACDYI010000000
VERSION     DACDYI010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.059"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            168..362
                     /locus_tag="IWM52_002043"
     CDS             168..362
                     /locus_tag="IWM52_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974288.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="IWM52_002044"
     CDS             631..930
                     /locus_tag="IWM52_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974289.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="IWM52_002045"
     CDS             complement(1027..1461)
                     /locus_tag="IWM52_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9974290.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="IWM52_002046"
     CDS             complement(1693..2403)
                     /locus_tag="IWM52_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP9974291.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="IWM52_002047"
     CDS             complement(2393..3253)
                     /locus_tag="IWM52_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAP9974292.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="IWM52_002048"
     CDS             complement(3258..3896)
                     /locus_tag="IWM52_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAP9974293.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="IWM52_002049"
     CDS             complement(3911..4210)
                     /locus_tag="IWM52_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9974294.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="IWM52_002050"
     CDS             complement(4253..5737)
                     /locus_tag="IWM52_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP9974295.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="IWM52_002051"
     CDS             complement(5757..6218)
                     /locus_tag="IWM52_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9974296.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="IWM52_002052"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="IWM52_002052"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAP9974297.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="IWM52_002053"
     CDS             7521..8291
                     /locus_tag="IWM52_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9974298.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="IWM52_002054"
     CDS             complement(8564..9391)
                     /locus_tag="IWM52_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9974299.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9959..11194
                     /locus_tag="IWM52_002055"
     CDS             9959..11194
                     /locus_tag="IWM52_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP9974300.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12193..12453)
                     /locus_tag="IWM52_002056"
     CDS             complement(12193..12453)
                     /locus_tag="IWM52_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP9974301.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12480..13781)
                     /locus_tag="IWM52_002057"
     CDS             complement(12480..13781)
                     /locus_tag="IWM52_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9974302.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14073..14531)
                     /locus_tag="IWM52_002058"
     CDS             complement(14073..14531)
                     /locus_tag="IWM52_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP9974303.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14544..15869)
                     /locus_tag="IWM52_002059"
     CDS             complement(14544..15869)
                     /locus_tag="IWM52_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAP9974304.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15886..17298)
                     /locus_tag="IWM52_002060"
     CDS             complement(15886..17298)
                     /locus_tag="IWM52_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9974305.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(17315..18079)
                     /locus_tag="IWM52_002061"
     CDS             complement(17315..18079)
                     /locus_tag="IWM52_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAP9974306.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18183..19058)
                     /locus_tag="IWM52_002062"
     CDS             complement(18183..19058)
                     /locus_tag="IWM52_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9974307.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19313..19397)
                     /locus_tag="IWM52_002063"
     tRNA            complement(19313..19397)
                     /locus_tag="IWM52_002063"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19361..19363),aa:Leu,seq:caa)
     gene            complement(19415..19486)
                     /locus_tag="IWM52_002064"
     tRNA            complement(19415..19486)
                     /locus_tag="IWM52_002064"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19452..19454),aa:Gln,seq:ttg)
     gene            complement(19496..19568)
                     /locus_tag="IWM52_002065"
     tRNA            complement(19496..19568)
                     /locus_tag="IWM52_002065"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19533..19535),aa:His,seq:gtg)
     gene            complement(19630..19714)
                     /locus_tag="IWM52_002066"
     tRNA            complement(19630..19714)
                     /locus_tag="IWM52_002066"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19678..19680),aa:Leu,seq:caa)
     gene            complement(19735..19805)
                     /locus_tag="IWM52_002067"
     tRNA            complement(19735..19805)
                     /locus_tag="IWM52_002067"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19771..19773),aa:Cys,seq:gca)
     gene            complement(19851..19922)
                     /locus_tag="IWM52_002068"
     tRNA            complement(19851..19922)
                     /locus_tag="IWM52_002068"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19888..19890),aa:Gln,seq:ttg)
     gene            complement(19934..20006)
                     /locus_tag="IWM52_002069"
     tRNA            complement(19934..20006)
                     /locus_tag="IWM52_002069"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19971..19973),aa:His,seq:gtg)
     gene            complement(20009..20082)
                     /locus_tag="IWM52_002070"
     tRNA            complement(20009..20082)
                     /locus_tag="IWM52_002070"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20048..20050),aa:Trp,seq:cca)
     gene            complement(20089..20169)
                     /locus_tag="IWM52_002071"
     tRNA            complement(20089..20169)
                     /locus_tag="IWM52_002071"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20133..20135),aa:Tyr,seq:gta)
     gene            complement(20171..20246)
                     /locus_tag="IWM52_002072"
     tRNA            complement(20171..20246)
                     /locus_tag="IWM52_002072"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20211..20213),aa:Phe,seq:gaa)
     gene            complement(20282..20354)
                     /locus_tag="IWM52_002073"
     tRNA            complement(20282..20354)
                     /locus_tag="IWM52_002073"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20319..20321),aa:Asp,seq:gtc)
     gene            complement(20364..20436)
                     /locus_tag="IWM52_002074"
     tRNA            complement(20364..20436)
                     /locus_tag="IWM52_002074"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20401..20403),aa:Val,seq:tac)
     gene            complement(20452..20523)
                     /locus_tag="IWM52_002075"
     tRNA            complement(20452..20523)
                     /locus_tag="IWM52_002075"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20488..20490),aa:Glu,seq:ttc)
     gene            complement(20538..20627)
                     /locus_tag="IWM52_002076"
     tRNA            complement(20538..20627)
                     /locus_tag="IWM52_002076"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20591..20593),aa:Ser,seq:gga)
BASE COUNT         6530 a         4319 c         3276 g         6539 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagaaccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgtccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatgcttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat
     9481 aaaaatttta attttaaaat aatattcaaa ttaaacaact ggcgtatctg ctccttgatt
     9541 tctttttgtg ttatctgaat aattatcgtt tacagttata acacatagtg aaaattgtca
     9601 tggcgagaaa gtctaagaat atttttaaac ataactattt ctttcttacg tgtgtataat
     9661 gacgacagca cacctatcaa ttaatataat tattttattt ttttatgttc tcatgtttat
     9721 ttttaatgac acaacttctc gtatttatct ggcacaacat ataaatcgat caaaaaattg
     9781 ttgcgtgttt ttttgaaaat tggagtttca acttgattac aaaatttaaa tttagaagtt
     9841 gtgaaaacag cgagagacag tttaatattc aaaatataca taggctactc gaacgctgtg
     9901 ttttttagta tactagtaag aaacgaaggg atagactcat tccaataagg aggatttcat
     9961 gaagacaatt ctttttgctc ctgcagtatt caatttagct gagacaacac gaatgattga
    10021 ggttgccaaa gctatgaaag accgttatta ctgtgtattc tttggctttt ctaaaacttt
    10081 tgctcctttg atcacagaaa gtggctttga gtatcattta ctagcgccga tccttacgaa
    10141 aaaacaagaa gaacaaatca taaaatttga ccagttgcgt acacttaaaa acccttttac
    10201 caaagaaatg gttgcctctc gtatcactag tgaacagagt ctgattgcta agtttgagcc
    10261 tcgactgatt gtgacaggtt cgaatgtgac aattttttta tcggcccgca ataaaaagat
    10321 tcctttagta tatgttaaac cctatgcttt gagtgaacct ttttttcaat cagagcatgc
    10381 gctgatgccc cacgagttac gtttcttcgg ccctttaagc aatccccttt ggcactggac
    10441 aaaaaagcgt atagtaaata cccgatggat ccccaatgcc ttcaaacacg ctgcacaaaa
    10501 agaaatgctt cctttaccaa aaacaagttt acaaatgatg gatgctgatc taaatctcat
    10561 cacaacgcct aaactatttt tggaagaaga acagctgccg gaaaattatc gaatcgttgg
    10621 tccaattttt gctaagctca gtacttcctt gcctgaagaa gtgctaactt ttatacagcg
    10681 aaagcaacaa gaaagaaaac gtattatcta tttcgcaatg ggtagttcag gcaatccgcg
    10741 tatgttacgc aaaattttgt tcttcttaca aaaacaaccg gatctagcgg ttattgcacc
    10801 tattgactat ctgcttgatg acattcaatc tgactcagaa gatcttttaa taacagaata
    10861 tctgtctagt catcttttgc atgaattcat tgatttttct tttatccacg gcggggaagg
    10921 gaccgtccaa actgcctgtg aatccggcaa accgtttatc ggtgtgggga tgcaatttga
    10981 gcaaagctgt aatattgact attgtgtaaa atacggaaac gcaatcgccc tcacaaaacc
    11041 agtgactgaa agaaaactgc agattgctct gcgcgaagtt tcttcaccca agattcgtca
    11101 gcaggcacgc acgctgcaag ccgtttttcc aataaatggt ccacaacaag caattcaaga
    11161 aatcgaaacc ttcctgagca accacccaat atagtttcaa gtagaaaaag cttttttaat
    11221 agagactcat cagtaatttc gctattcgag acccagacag tgaaaataca aaaaaagtaa
    11281 atagtggatt gaaaagttag agttacttaa attacggtgt ctaaaaaaca caaaatcatc
    11341 cttaaaataa aagatctgac gctatccaaa tgagcatagg tagcgtctgc catttattct
    11401 tagtcaaatt ctttgacacc tttctctggt tggatcatct caacgtttct tcaaaagaaa
    11461 gaccataata attaggcaaa tacattttct taaaatgtgt catatagatc tagaagcatc
    11521 gctttgtaga gatttgtgcc gctagtcccc tcaacaatta ctaaatcaaa tgtaggattt
    11581 accactttat ctttgataat caacctccct cttctgttga gacaatgatc tcgcaatccg
    11641 atgcatactc ctatataaac tgccagcaat ccccacatat tgagtagatc atgtcagaat
    11701 gttctatgtc gtcagcaacg atacactaaa agtcacgttc accatgactg attgctattc
    11761 ccagcacaat tatttctata cacgtttcat gatttgaata aacactcaca tcatacatat
    11821 taccattcat acaccatact gcagccccta ctatatggtt gtaagctccc aaatctatat
    11881 ttgacttgat cactttggta gtagatctac taattctaaa tccattgctg ataaaatttc
    11941 cattttttcc tccttctttc tgccaaaaca gttaaaacaa taataatctt tatgtaaaaa
    12001 agggctgtga caaaagcctt gtcacagtcc catattccga ataaacggtg gtcaaaagct
    12061 tgcatctgcg gtaataagtt caaccaaacg aaaaataagc caacatttca caaaaattta
    12121 agaagcaatt ttcgtaaatt tcttcttatt tcttgaagct aaccacttct gttacaacca
    12181 cttttagaac atttaaagct tcgatgtgac aaaccatcta tgctgctcga tcataaaatt
    12241 gatagcaaaa attccacaaa gtacatatgt cccccatgaa aacaaaacat tcatatgcgg
    12301 tacaatgatc tttggaagaa tcacgatcaa aaatgatgcc agtgcccaaa tccatcgatt
    12361 gatttgaata tgatatgtag ctagcatatt caatataagg tgactagccg ttaataaaaa
    12421 gataaaaaca aaaaataaaa tcagccaata cattgtttcc caccttctaa aaaccaaact
    12481 tagacagtct ggacttcgtt ttctccacga ctttgttcat ctgacagcat ctttttatca
    12541 taagctttta caaatggata atagataata aatgctatca atgtgttgat catgatcaag
    12601 ataacaacac gccagtcatt tcctgctgaa agataaccgc cgattggagc tggtaatgtc
    12661 catgttggca aaacagtaaa tctattgacc atttctaatt tgactgcaaa ataactgata
    12721 atagtcaaaa ctaaaggaac taagttgaac gggatgaaca tgtaaggatt caacatcata
    12781 ggagctccaa aaattatggg ctcattgata ttgaagatac caggaataat agaaaaacga
    12841 ccaacctctt tcaagaattt tgatttacag aagaatataa acaaaacaga taccacgatc
    12901 gttgctcctg caccaccaat tgttaccgtc cattgataaa actgttcagg agcgatatag
    12961 ggtaattggg tggcagctgt accatctgcc aatgcctttg cattctcatc caacatgatc
    13021 agccacattg gacgaacaac agcccctaca atactcatgc catggatacc aaatgaccac
    13081 aacaaatgga tcaaaaacat tggtagtaaa acaccaagca aattattccc taaaatattg
    13141 gttaaaggct cgaataacga taataaggca gcgtttacat caaagcccgc gacgcttcga
    13201 atcagccaag taattgttaa aatgactgca cctggaatca atgcctcaaa actacgggca
    13261 acagcaggtg gcacttgttc tggcattttg atagtaatat ttcgattttt aaagaaacga
    13321 tatacttcca ctgcaaaaat catagataag atccccgcaa acatcccaga tgctcccaaa
    13381 gaactcatag gtaagaccca gccaagaggt gcctcttcag gagttaaaac gtttacagga
    13441 acttgtaaca taaagaacgt tcctaaagat aatataccac ctgtcacact atctaatcca
    13501 tatgatttag ctagatccga acccataaca aaagttgcat aaatagccat caatcccatc
    13561 gtcatcctaa agggtaaaac aatcgttgcc gaataagggg caataaattc attccatcca
    13621 ggaaacggta aattgataaa gaccataaaa aatgtcccaa tcaaaatcaa tgacaatgtt
    13681 gaaacaaccc cattacgaat tgctaataaa tgacgctgtg taccaatttt tgccattggt
    13741 gggacaagtt tctcttcgac ccatttcatc agcttttcca ctaaaaccac tccttttcaa
    13801 taaaatatga accattcatt gcatgacaac gcaaagaatg ttcataacta attatataat
    13861 taagaaattt tatttcaata tattctctta aataatcaaa tttattttta agaatcctga
    13921 aaataagttt tctaaatcat aggagcaaca aaaaaattcg ataattaaca aaaatcaaaa
    13981 tgattaaatc tagtgattga aacatatcat ttcaaaaaaa ttttgtaaat tcacccataa
    14041 atatcaccct atttaacttt tttcatcgta cactaaaatt caaaacaact atctttaatc
    14101 tttacttctt ggttttgttt taataaattt ccttcaaaaa tgcctggaga aactacaatg
    14161 atcaccgtat ctttttgtgc ttgctccaac ttcttcaaat ctataatagc taacagtgtc
    14221 ccttctttta cctcctggtt actttcaacc cgtatctgaa acggacttcc ttcgagttca
    14281 actgtatcta aacccatatg gatcagtatt tttctgccag attcactttc gatcgttatg
    14341 gcatgtcggg taggaaatat attatcgatt attcctttaa ctggcgcatg gatatatcca
    14401 tcatgattcg ttatagcgta tccaactcct atcatttttg aagaaaagac ctcgtctttt
    14461 actgctgtta atggaataat ttgaccacta gctacagctt ttagaatgtt tcttgtttta
    14521 aagaaatgca ctttttatca cctctacttt aatgctggcc aatatttttt gtttactttg
    14581 atcatttcat ctagtatttt ttttgcgact ctggcatttg gtacggtttg attcaatgta
    14641 aatgcttgta aagctttttg ataagaacct tcaaagtaag catctactag taacttctct
    14701 actgcaactt gtgcctccat caatccttta tgaaaatcac tgattggttt tcttagtgta
    14761 attggctcca caccagttgc accaacatat gccggtattt caacaactgc atctgaccgt
    14821 agattcggaa tcgccccttg attaggaacg attagcataa agcgacgacg atcatcattc
    14881 aacaaggaaa tagctatatc gacgatatat tgtccatgct ccccaaaatt aaagttcaat
    14941 acttcttcca caataccgcg gcgtatttta tcagccattt ctttggtatt tttctctcgt
    15001 ccatccatga ccatattagc acgtgtatac tctttgtcag tatgctcaac gaccatatca
    15061 ggatataagt aatactctaa gtaattattc gggatattac ttggaaaatt tttcgtgatg
    15121 actgacatca tttgaaaggt tttttgccaa gttttatctc ctatattaaa atcggcgact
    15181 tgcatttctt ttgttattag tttttcaata atctctggca taatttctcg ttgcaattct
    15241 ttatcataaa tcgatgtata ccagccaaag tggtttaaac cataatacgt aacgatccaa
    15301 ttcttacgat cataaccgta attcactgca atcgtttctt caattgaaat agtcatatca
    15361 caggcattaa tcatttttac attcggatat ttacgacgaa cagcttcaga aacgatcgac
    15421 tctggatttg tataattcaa aatccatgct ttgggggcat agtcttgtat atgatcgaca
    15481 atttccaata aacctttgat tgagcgcatc ccataagcaa atcctccgag tccacacgtt
    15541 tcttgtccta ctaatccatg tttaagtgga atcttttcgt ccttttctcg catctccaat
    15601 cctccaacac gaatttgtga gaaaacaaaa tcgcatcctg taaaagcaag ctttggatca
    15661 tccgttgcac gaatgcgaat atttgaaaaa ccttttcttt tcagcataaa tttaacaatc
    15721 aagtacatat ccatatttct ttcttcatca atatcatata aacgtatttc agataatggc
    15781 aaccgttttt cattgttcaa aatggcttga acgattcctg gtgtatacgt actcccgcct
    15841 ccagctatag tgataatctg ttttttcatc atcatctttt cctttctatt cttctaataa
    15901 tttattgaat tctgccataa cataagtcac ttctaagccg atgataattt gataattttt
    15961 ttcagttggt ttaatgatcc cgcttatccc taattgcttg attcgctgtt cctcgaccat
    16021 ggtgggatcc actacctcta cccgtaggcg tgtattacaa ctatagacat ccaggatatt
    16081 ggcttttcct ccaaacgctt gtagcatttt tgttgccata tattcataat ttttacttgc
    16141 taaccctaac tctgtttctt ctaaggttac ttcattgcca aactctgcct tagcttcacg
    16201 tcctaatgtc tgaatgtttc tcttattgat caaataataa aaagtaacgt aatacaaggc
    16261 aaaaaagaaa attccgattg gaagaatcaa aatagcattg gtagcgattc gaaaattgat
    16321 gagataatca acaatacatg ctccgaatgt aaaaccaatt cgtatattga ataagtaaca
    16381 gacaatccct gctaaaccgg caaaaagtgc atggatcacg tacaacatag gagaaagaaa
    16441 catgaaacta aattcaacag gctcagtgat atttgcaata aatgaagttc ctgcaccgct
    16501 tcccatgatt cctttgacca tctctttatt ttctttataa gctgctttat agatagctag
    16561 acatatagct ggtataccaa acatcattac tacaaaaaaa actgataaaa acagtccagc
    16621 ctgaggatct ccgttgataa agcgtgtcat ctctcctctg aaaacttcct tgctaggaga
    16681 tgtatagctt cctaaatcgt aataaatata cgtattcaac acatggtgca gtccaaaggg
    16741 gatcagcaaa cgattcaaaa acataaatat ccccacacct aatgctccca tatcaacgag
    16801 cagttttgca aaattatcaa tcccaatttg gatgagcggc cagataaagc ccaaaaaaac
    16861 agctgcgatt gtttgtagaa tcatcaccat agtcaatggg aatttttcgc ccgcaaaata
    16921 agaaaaaact actggtaatt tttgttcttt aaagcgatta tacgtccatg ccgctactaa
    16981 accggaaata attccaccaa aaatccccat attcacttct gggtccataa tttctaagcc
    17041 ttttttcaag gtagcaatcg ctaaaaaacc agtaagtgca ggtattcctt tgtccttatt
    17101 tttgacaaaa ccgattgcaa ctcctatagc aaataagata tccaagtttc caaaaacggc
    17161 atttccagct gcaccaatta ctgggatgtt cagcatatcg tcagctgata aacgcatgat
    17221 caatcccgct aatggcatcg ctgcaactgg aagtagcatc gctcttccaa gcttctgcac
    17281 atttgattta acattcattt ttattcctcc tctattaaat atcctcatat gttactaact
    17341 caatcaaagg atcatttttc actttttgag caaatgctga atgaattaaa aattcgactt
    17401 ggtgggtacg aggatacaca aaacttgaac catttaacaa ttcctgatcc aaatacgctg
    17461 cgtgacacat gacttcgatc gttttatctt ctctcaatat catgagtaaa cttgccaaat
    17521 attcttccac actcgtattg atttgtcgtt ttttttcatc agtttctcct acgacatcga
    17581 aacaaggctc aaaataatcg acatgacgaa cttcatcttt tcgttcaaag ttgctccgaa
    17641 ccggcaaatc atattttttt gctaatgtaa caactacctc ctgattttca tccaaagtat
    17701 gagtatgatg atgactgtct aaatgagttg gtatgatccc cgcatgataa attttttgaa
    17761 tctgagcatt ccattcctga tacagttgat ctttgtcaat gttaaatgga tgctgataat
    17821 aagataagga tctgaactct ccgctttcga taagagtatc gacagttttg aggagtggtt
    17881 tgccacacgt caatgttaaa tggactccta cacctagcct aggcatttcc tttcttagtt
    17941 tcacagcatg ctcgaaaccc ggcatatttg ccatcaatgt agttgatgtc aaaattcctc
    18001 gctgataagc atccataatc ccataattca caccatggct atagccaaaa tcatccgaat
    18061 taaaaatcac ttttcccatt tcattctcct tcttttttct actaaaatag gcgcaaccaa
    18121 ataatcatag tccatttact atgtgtcatt tggttgcgcc tatagatagt tacgccctta
    18181 ttttaataga ttcttgctag atgaattgcc aaatagatga tttcattttc ttgaatggca
    18241 agttgaaatt gctgttcgat gatatcttta atctttagtg ctgtacaata cgctttgcta
    18301 tgtttaaatg tttcagtaaa aaaaccaata ttttctactg gcgtttcttc ctgataaatc
    18361 aagcgtttaa ccaggaattt gatatgaacc attaagcgat gataagtcag tgattcttta
    18421 tcataaacgt ttgaatagga tttttctaga tgggacaaaa tcgtggtcgt catttctact
    18481 atttttaagc tctttaaact attaggctct cctagctcag cattaatgat atgcatagtg
    18541 atgaatccta cttcatttga ccctaactcg gtttgatatc gatgattgat tcgctcgacc
    18601 acttctactg ctatatcata ttcatttgga taaaggaatc tcatttctgg caacataatt
    18661 gctgtgacat ctctatcttc tctttttcgt tgaatagcaa aataaatatg atctgtcaat
    18721 gtaatataga tatttgaatc taatggagag gagatcctag ctgttatcag ttcaatcact
    18781 tcttccgcaa aatccataaa ttcgattgga atctgcgaga ctatctcact gaatcgtttg
    18841 atcttttctt tagaatctaa tacaaataat ttgtcagcat cctcaacatc aaatggctta
    18901 ctcactttag cagaaaagcc aatccctttc cccatcgcaa ttttttctat tccatcgtca
    18961 tcgatcaata cagcattatg attcagcttt ttcatcacat acaccgttaa ttcacctcct
    19021 ctatatgata aaacaaaaca acttgcagac tccttcatac atatttttgt ctccaaacaa
    19081 tttttaaaga caaaaaacga cctaagaaag aaccctttct taggtcgtgc ctaattgaat
    19141 agtagcaccc tacataattt acaaaaatag tttaacacat aataaaagcg ttatcaatat
    19201 cttttttact cgttttatga taatacttgt atcgaattga tttggaggta attacataaa
    19261 aacaaaaaaa atatagcaac tctctataag aaagttgcta tattgataat tataccggcg
    19321 gccggggtcg aaccggcacg tcctcaacgg acactggatt ttgagtccag cgcgtctgcc
    19381 aattccgcca cgccggcaaa tttaaaaata ataactgggg tagctggatt cgaaccaacg
    19441 catgagggag tcaaagtccc ttgccttacc gcttggctat accccaagaa taaagggcga
    19501 atgatgggaa tcgaacccac gaatgccgga gccacaatcc ggtgcgttaa ccacttcgcc
    19561 acattcgcca tcataaaaga tgttgtttaa tttctcaata aaaaactaag atgaagacat
    19621 cttagtcaat accggcggcc ggggtcgaac cggcacgccc tcaacgggca ctggattttg
    19681 agtccagcgc gtctgccaat tccgccacgc cggcaagttg atttattagt actaaggcgg
    19741 taaccggatt tgaaccggtg atgaaggttt tgcagacctc tgccttacca cttggctata
    19801 ccgccattat actattatca atctataaaa taaagaataa attatagaaa ctggggtagc
    19861 tggattcgaa ccaacgcatg agggagtcaa agtcccttgc cttaccgctt ggctataccc
    19921 caagaataaa aagggcgaat gatgggaatc gaacccacga atgccggagc cacaatccgg
    19981 tgcgttaacc acttcgccac attcgccatg gcaggggcag caggaattga acccacacta
    20041 acggttttgg agaccgctgt tctaccttta aactatgccc ctataaaatg gaggagagtg
    20101 gattcgaacc actgaaccct aaggaacgga tttacagtcc gtcgcgttta gccacttcgc
    20161 tactcctcca tggtggcgcg ggacagaatc gaactgccga cacacggagc ttcaatccgt
    20221 tgctctacca actgagctac cgcgccatat tttaaaatta ttaattgaat aacaagaacg
    20281 acggttccga cgggaatcga acccgcgatc tcctgcgtga caggcaggca tgttaacccc
    20341 tacaccacgg aaccagttca ttatggaggt taacgggatc gaaccgctga ccctctgctt
    20401 gtaaggcaga tgctctccca gctgagctaa acctccaaca ttcaattaaa atgacccgta
    20461 cgggattcga acccgtgtta ccgccgtgaa agggcggtgt cttaaccgct tgaccaacgg
    20521 gccaggtaaa tttgctacgg agagtaaggg attcgaaccc ttgagacagt ttggcaccat
    20581 ctacatgatt tccaatcatg ctccttcggc ctctcggaca actctccaga gtgaaagaag
    20641 ctcaatactc tttctcaaaa tgag
//
LOCUS       DACDYI010000060        20457 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.060, whole genome shotgun sequence.
ACCESSION   DACDYI010000060 DACDYI010000000
VERSION     DACDYI010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.060"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..337
                     /locus_tag="IWM52_002077"
     CDS             <1..337
                     /locus_tag="IWM52_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAP9974308.1"
                     /translation="LAESDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEI
                     AAKAIMGSCQKFLSSDKNPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIE
                     KDLEMLNKK"
     gene            complement(416..1972)
                     /gene="rny"
                     /locus_tag="IWM52_002078"
     CDS             complement(416..1972)
                     /gene="rny"
                     /locus_tag="IWM52_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAP9974309.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(2383..3432)
                     /gene="recA"
                     /locus_tag="IWM52_002079"
     CDS             complement(2383..3432)
                     /gene="recA"
                     /locus_tag="IWM52_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAP9974310.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(3541..4782)
                     /locus_tag="IWM52_002080"
     CDS             complement(3541..4782)
                     /locus_tag="IWM52_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAP9974311.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(5022..5600)
                     /gene="pgsA"
                     /locus_tag="IWM52_002081"
     CDS             complement(5022..5600)
                     /gene="pgsA"
                     /locus_tag="IWM52_002081"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAP9974312.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(5809..6693)
                     /locus_tag="IWM52_002082"
     CDS             complement(5809..6693)
                     /locus_tag="IWM52_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9974313.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(7471..8757)
                     /locus_tag="IWM52_002083"
     CDS             complement(7471..8757)
                     /locus_tag="IWM52_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP9974314.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(8750..10018)
                     /locus_tag="IWM52_002084"
     CDS             complement(8750..10018)
                     /locus_tag="IWM52_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP9974315.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            10270..10731
                     /gene="mscL"
                     /locus_tag="IWM52_002085"
     CDS             10270..10731
                     /gene="mscL"
                     /locus_tag="IWM52_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAP9974316.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(10790..11236)
                     /locus_tag="IWM52_002086"
     CDS             complement(10790..11236)
                     /locus_tag="IWM52_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9974317.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(11329..11574)
                     /locus_tag="IWM52_002087"
     CDS             complement(11329..11574)
                     /locus_tag="IWM52_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974318.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(11689..12267)
                     /locus_tag="IWM52_002088"
     CDS             complement(11689..12267)
                     /locus_tag="IWM52_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9974319.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(12344..12874)
                     /locus_tag="IWM52_002089"
     CDS             complement(12344..12874)
                     /locus_tag="IWM52_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAP9974320.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(12878..13738)
                     /gene="rlmB"
                     /locus_tag="IWM52_002090"
     CDS             complement(12878..13738)
                     /gene="rlmB"
                     /locus_tag="IWM52_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAP9974321.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(13731..14126)
                     /locus_tag="IWM52_002091"
     CDS             complement(13731..14126)
                     /locus_tag="IWM52_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP9974322.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(14123..15535)
                     /locus_tag="IWM52_002092"
     CDS             complement(14123..15535)
                     /locus_tag="IWM52_002092"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAP9974323.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(15532..16068)
                     /gene="cysE"
                     /locus_tag="IWM52_002093"
     CDS             complement(15532..16068)
                     /gene="cysE"
                     /locus_tag="IWM52_002093"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAP9974324.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(16390..17850)
                     /locus_tag="IWM52_002094"
     CDS             complement(16390..17850)
                     /locus_tag="IWM52_002094"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAP9974325.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(18020..19177)
                     /locus_tag="IWM52_002095"
     CDS             complement(18020..19177)
                     /locus_tag="IWM52_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAP9974326.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(19211..>20457)
                     /gene="radA"
                     /locus_tag="IWM52_002096"
     CDS             complement(19211..>20457)
                     /gene="radA"
                     /locus_tag="IWM52_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAP9974327.1"
                     /translation="TDRRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGG
                     VVPGSMVLIGGDPGIGKSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSIN
                     TEFYLYAETDMNEISRAIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLK
                     TAKTNGIAIFIVGHVTKEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGST
                     NEIGIFEMHEHGLEEVANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTM
                     FGNAKRTTTGLDFNRVSLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVS
                     IASSYKEKGTQPTECFIGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWE
                     APKGIEIVGVATLAETLKRVFR"
BASE COUNT         5410 a         4498 c         3680 g         6869 t
ORIGIN      
        1 tttagcagaa tcagatttca gtacattcat tggccttgct ggcagctcac ccgcattcgt
       61 ttacatgctg atcgatgcga tgggacggac tggtgtactg aatggtttgt ccaaacagac
      121 agccactgag attgctgcaa aagcaattat gggttcttgt caaaaattcc tttcaagtga
      181 taaaaatcca tgggaacttg tagaccaagt ttcttctcct ggcggtacaa ctgttgcagg
      241 tgtagttgcc ttagaagaag caggattcat cccagcaatc atcaaaggaa tcaatacgac
      301 gatcgagaaa gatcttgaga tgttgaataa aaaataatga tagcaatgtt ttttcgtgcc
      361 ttacaaaaaa agaagagctg tggcttttgc cgcggctctt cttttgtatt ttttattatt
      421 tagcataatc agttgcacga gtttcccgaa taacgattac tttgatgtgt ccaggataat
      481 ccagttcttc ttcgattcgt ttacgaatat cacgtactaa acgaacagcg tctaaatccg
      541 agatttcttc tggcttgacc attacccgaa cttcgcgtcc agcttgaact gcgtaacttg
      601 attctacacc ggcgaagctg ttcgaaatat tttccagact tcttaaacga cggatgtagt
      661 tttctaatga ttcacttcgc gcaccaggtc ttgctgctga gagcgcatct gctgcagcta
      721 caaggacaga gataacagaa gtagcttcca catctccatg atgagaagcg atcgcgttga
      781 ttactactgg gttttctttg tacttggcag ctaattccgc accgatctca acgtgagatc
      841 cttcaatctc atggtcaagt gctttcccaa tatcatggag caacccggca cgttttgcta
      901 attggatatc ttcgccaagc tctgctgcta agataccagc taattttgca acctcgaccg
      961 aatgcttcaa gacgttttgt ccataactag ttctgaatcg cagacgtccg atgatcttga
     1021 ttagatctgg atgaagtgta tgagcaccga cttcaaacgc tgcctcttcc ccgtattcgc
     1081 gaatacgttc atccatttct ttacgagatt tctcgaccat ttcttcgata cgcgctggat
     1141 ggattcgtcc atcttgtatc agtttttcca aggtcatccg tgcgatttct cttcggattg
     1201 gatcgaatcc ggaaagaaca actgcttccg gtgtatcatc gatgattaaa tcgataccag
     1261 tcagcgtctc aagcgttcga atattacgac cttctcgtcc aatgatacga cctttcattt
     1321 catcattcgg aagggtaacg actgaaacag tcgtttctga aacttgatct gctgcgcagc
     1381 gttgaatcgc taatgataat aagtttttcg ctttgcgatc tgcttcttct ttggcacgct
     1441 gttcagattc ttttaccatt agagttaact catgattcag ctcttcttca gtcgatttca
     1501 tgatgacatc tcgtgcgtcc tcttttgata aaccagcaat tttttctagt tctttttgtt
     1561 gatcttcaat cattttttca acgtcttttt ctcgctcttc aattaattgc tgttttgaac
     1621 taagtgcttc ttctttactt tcaagtgagt tctcgcgttt ttctaacgag tcatctttac
     1681 gattcagcgt ttgttcgcgt tgaatcaaac gattctcttg agattttaat tctaacttac
     1741 tttctctcag ctcactttca acttcagaac ggtatttctg attttcttcc ttagcttcta
     1801 acaatgcttc tttttttaga gtttctgctt ctttttttgc actatcgatg attccagcag
     1861 ctgatgattg tgcaccagct atttctttct cgtgacgtga ctttgcgacc ataaacccta
     1921 aaccaagacc gacaattaaa ccgatgatag cgaggagaat gttgaatacc ataaaatcca
     1981 cctccatact atctttttca tttttatcat ctatacagct tctatgataa attattctaa
     2041 actcgtcaat atgcatcttc gcatatatgt gtacaaaaaa catgcacaac tttattctaa
     2101 tgtttgtacc ccgtagtgtc aactttaaat aaacaaagaa acggctttct acttattcat
     2161 taaacgatgg tttttaaaat aaatgacact agtttcatat aggtaaacct ttaaattgag
     2221 aaacttttct attaaattat tctttgtaga tgaacagata tttttatttt atgtacccac
     2281 tcgtataatt aaataactca cgatttcttt attcaataaa aaaacggcag gtcaaggaga
     2341 gtacctactc ttcagaccag ccgtttttgt ttatcataat ttttattctt ctactaaagg
     2401 caattcttct tgtccttttt catcttctgg gacagcgact tcttcaccaa taccataagc
     2461 tgctctaact agagcagaaa cttctgccat catttcagga tgattagcca tatagatttt
     2521 agcgttttcc cgtccttgac caatccgatc ttctttataa gaataccaag caccgctttt
     2581 atcaacaatg tctttttcta ctgccatgtc tagaagttct ccttcttgtg agatccctaa
     2641 accatacatg acatctactt cagcgatttt gaaaggaggt gctactttgt tttttacgac
     2701 tttgatcttt gtacggttac caacaatatc tgtaccttgt ttcaattgtt ctgcacgacg
     2761 tacttccaaa cggatcgttg cgtagaattt caatgcgcgt cctccaggag tgatttctgg
     2821 attcccaaac atcacgccta ctttttcacg gatttggttg atgaaaatag cgattgtttt
     2881 tgttttgttg atcgaaccag agagtttacg caatgcttga gacatcaaac gtgcttgtaa
     2941 cccgacatgt gagtcaccca tttcgccgtc gatttctgct cgtggaacta aagcagcaac
     3001 tgaatcgact actacgatat ctacagcccc acttgatact aaagcatcag cgatctctag
     3061 accttgttct cctgtgtcag gctgtgaaag aagtagttca tcgatattca cacctaattt
     3121 ttgtgcatat tgcggatcta acgcatgctc agcatcaatg aaagcggccg ttccgccatt
     3181 tttttgtact tctgcaatag cgtgtagtgc aactgttgtt ttacctgaac tttcaggtcc
     3241 atatacttcg atgatacgtc cacgggggta accgccgaca cctaaagcga catctaacgc
     3301 aagagagcca cttggaattg tagagatttg ttggtcgatt ttttcgccca atttcataat
     3361 agagccttta ccatagctct tttcaatttt tttcagtgca gcatctaagg ctgctttacg
     3421 atcatctgcc aatacgataa tcctccttat atttacaaat cagagcaaca tttcattatc
     3481 cttatcatat cttttttagt gaaaaaaagc aagaaaaaac cgaacaaata ttcgcattta
     3541 tcattttctt aaaagctgtt ttctcaaaag atcaaatcct tgcataaccg cattatatct
     3601 gatagcagca cgatctctgg caaagtgata tttttccgaa accacgccgt ctttagatga
     3661 aatagcgatc catactgttc ctgcagattg accttctaac gtgtccggtc cagccactcc
     3721 ggtgaaagct acactgtaat ctgttcttgc tatctcacgt gcttgaatgg ccatctgctc
     3781 cacacattct ttgctgactg ttccatattc tgctaatagc tccggatcaa tctccagaaa
     3841 accagctttt gtttggagcg agtaggtaac gaatccgcct ggaaatactt cagatacacc
     3901 agcaatgtcg cctaatgcgg cttggaaggc gccggctgtc aaactttccg ctgctgttac
     3961 agtctgcttg ttttctttca gtagttccac tacgactttt gctagcgaat tatcatcgcc
     4021 gtatccataa aaatattccc ctactcgttc ttggattttt tcttctaatg ctaaaagagc
     4081 ttgattccct gcctgaacat catttgtttt cactgtcagt cgtaatgtca cttcatttgg
     4141 tttggcatat ggtgcgatcg ttggattgat ctgtgtctca atcagatctt ttagctcagt
     4201 gaccagtcgc gattcaccaa tcccataaaa gcgcaaaact ttagaaatta gtttttcttc
     4261 agaaggaaaa cgttcctcta gtaaaggacg tacttgttcc acaaacattg gtttcaactc
     4321 attaggcgga ccgggaagaa ggatatacgc atgtgtatct gtttgataaa aagtccctaa
     4381 agcaagccct gtacgatttg gtagaggaac cccgccttcg ataatctggg actgttgcag
     4441 attgtttttg gtcatttttc gatgggttgt ttcaaaatat gccaataatt ttttgtatcc
     4501 ttccgtattt tgtatgagag acttaccgag gtgagccgca gttgcttctt ttgtcaggtc
     4561 atcttctgtc ggtcctaatc cgccacataa gagtatgagt tcacttcttg tttcagctag
     4621 agcaatcaac tcttctaaac gctgctgatt atctcctaca actgtttgat aataaacttc
     4681 gatacctaga tcagctagct gttctgacaa gaaagtggca ttcgtattca cgacttgtcc
     4741 tagtaaaagt tctgttccga cagctattat ttctgctttc attacttccc gctcccttat
     4801 atagtatacg ctgtattttt atattttttt atttaaaaag agggcaagga gctgtgacaa
     4861 aaatcttgtc acagctcctt gttccaaata aacagtgttc tcctgccaaa acctcggtca
     4921 taagtcacaa tatacataaa acatgaaaag ctgttttttg aaattatccc ttgtgactca
     4981 gagttagacg gcaggctcac aacctctcca ctaaacgata cttacattga tcctttgaac
     5041 acaccgctgt ttttgatgaa gtaatccaca cctgaataga tagtaaagat cagacaagca
     5101 taaagcatga tttgatctac tggaataccg atcaggctga atgggatatt attgataaac
     5161 agcaagataa tagcaaccat ttgtgtagcc gtcttgactt ttcccggcca agctgcagcc
     5221 atgacttctc catgttcgac aagtagtaaa cgcaagcctg tgacggctaa ttcacgacag
     5281 acaatgatcg ctactaccca accaggggcc tttccttggt caaccaacat gataaaagca
     5341 gtcataacta gcattttatc tgccaacggg tccgcaaact tgccaaaatt agtgacaagg
     5401 cctcgcgcac gggcaatttt tccatctaac cagtctgtga tactagctaa agcaaaaata
     5461 actgcaccta ctagctgtgt gacagccagt gtggtgtctc ctacgttaaa ttgtccccaa
     5521 tctaaaggta aacctacaac caaaatgaaa atcgggatca tacaaattct aagtacagtt
     5581 aatttatttg gtaaattcac actatcgact cctttcttcc ctattctacg tgactatcag
     5641 cgaagtttcc accgatatta tttacttttc caaatgaatt tattcaaagg agtattttta
     5701 ttgaaattga tctttttgaa ttaacgaaga aggaccgcga cacagatttg tcacgatcct
     5761 tcttttttat acctacaagt aaacagatgc gtaaactaat ttatacgatc attctgcgta
     5821 ttgaagagta aggttcacat tttttggact ctgattgttt tcccctggat taactggtac
     5881 ggattctcca ttcgctttga tcgttgcatt gcttgctgca ccaatcgtga tggtcgcatt
     5941 tgttgctgtt tccggtaaca cggtactctg tgtttcattt gctgccagtg tcccttgata
     6001 aacataggca ttgtctacta gcacacctaa ccatacacga ttgacagcag taaattcaag
     6061 ggttacaggc ttggtagcgt ctgtgatatt tactgcgatg gcactgccag aatcttctcc
     6121 tgtagctatt gccattttct tttcttcact tgattttgtt gttgattcta ctgtagaaga
     6181 ctctttggtc tgcgtgcttt gctctgagga agaggactcc tcgactgatc cttctacttt
     6241 aagcgaagaa gctgtgtctc cgattattgg atcagcttga cgatcttgcc aagtcatata
     6301 gcccacgaca acgacaatcg ccaaagcaac caatcctaat aaaatgacag gaagacttgt
     6361 ccaaaagcga cttggatgcg attcttcctg atgcattgct tttctagaag tacttactgt
     6421 ttcaggctgc ggacgttcag ggaccgatcc tgtcaatttg cttttcccat caaaaacatc
     6481 gactaaatgg tctccatctt ctcctacagc ttcagcgtat tgccgaataa atgcccgcac
     6541 gtaaaattta ccaggcaatt ggtcaaaatc atcattgtcg attgcttcca aataacgttt
     6601 ttgaattttt gtaatctgct gtaattcatc taaagatatt tttttattca ggcgtgcttg
     6661 ccgtaatttt tctccgatgg tttcaaaagc cactcgttca tcttctccag tctgtttgat
     6721 tctccgaagc atctgctgtt gcaacgaaag acgttatgtt tgggtgctgt gacaaaagtc
     6781 atgtcacagc tccttttttc cgaataaaca ttgttctcct gccaactcct cggtcacaag
     6841 tcacaatatc caataaatat aagaagctgt tttttgaact tgttccttgt gactcggagt
     6901 taaacggcag gctcacgacc tctttcttaa acggtgttca atcagctgtt cctcggtatt
     6961 agctcaaaaa ccggcaaaat ataaggagat atttttccgt atgtttctct aatactcagg
     7021 tcaaaacgct gatttcacaa cctcttcctt aaacggtgtt caaaagcttg catctgcggt
     7081 aataagtcca accaagttaa aaacattaag aagctgtttt tacttggctg ttcttattac
     7141 ttgatgcagg gcagcttttt cacaacctct tggtacacgg tgttccagaa gtagcttctt
     7201 cggcaataag ccgaaatatc acaaaaattt gagaagcaat tttcgtaaat tccttcttat
     7261 ttcttgaagc tgaccacttc tgtcacaacc tctttgatta cttcgtttga ttttatttta
     7321 atataagttg attaaaagag agcatgattt gttttatttt ctcactaatt tttccaacac
     7381 aactaagaat accataaaag ctgattttgg aggaggttcc tactacaagt ctaaaacgaa
     7441 atttaagact tttcctcata aaatactggt ttattttggt ctcatgtaaa actcgctgaa
     7501 ggcctcttca cttataaatg cttccccagc tgccagtacg tctttcattt ggatcgaatc
     7561 gatgatttca ggaagatcaa ataaagttcg atcgccaaat aaactttgcg taaattgatt
     7621 ggcaatatat tcgatcgaat ttaatgattg gaaatattgt ccaagcattt tcttcttcag
     7681 caaatcgaga tttgtttctg acacttccgg atcatcagcg aatccaagaa tgatttcttt
     7741 gactttttct gctgctttct ctggttcgtc cgtatctccg ctgaagtccg caaagtgaaa
     7801 ttcacgatcc aagctgaatt caaaaccaaa gctgtcatcg ataattcctt gattgtacat
     7861 cgctagatag tttcgagaag tattgcctaa aatcatttgg aacaacagat tgattgctgt
     7921 tttatagcgt aacagttctg ttccttcttg cggcaatgtg tctaatcctt taataccaag
     7981 tacaaattta tctcgtgtaa tggctgcttc caatgcactt tgtttgatga tttcttttgt
     8041 gttttcaggg aaataacgta caatttcttg ctttggaggg aaattttttg cttcttggtt
     8101 ttcccgaata agtttcatca atttctcagg ctccatctta ccaactacaa ataaaaccat
     8161 attgcttggc tgataaaacg tccgatagca cgtatacaag tcttgagctg tgattttgtc
     8221 gatactttca accgtgcccg caatatcgat atgcaatggg tgtgtaggat acaagttatt
     8281 tagtatgcca aaaaacatcc gccagttagg gtcgtcttca tacatttgga tttcctgacc
     8341 gatgattcct ttttccttgt ttacagtttc ttctgtgaag taaggtgctt gtacaaaatc
     8401 gatcagagtc gtcagattct tttctacctg atccgtcgta gaaaacagat agctggtttt
     8461 cgtaaaactt gtgaaagcat tggcggaagc tccttgtttc ccaaataatt gaaagacatc
     8521 accatcttct ttttcaaaca acttatgttc taaaaaatgg gcaattccat ctggaacttt
     8581 caccttttct ttttcaccat agggaataaa ttcgttatcg atcgaaccat aattcgtact
     8641 gaacaaacca tatgtcttgt gatagtcatt tttaggcaac aaatacacgg tcaaaccgtt
     8701 tggcagtact tcatgataaa gtgtttcatt gatttgctca tattctgttt tattcatttg
     8761 catttcctcc tgctaaaaag aagattgcct gcaattcgat ttgctcagct acttgttgta
     8821 tctctttgat cgttacgctc tccatacgtt tcaaccattc ttcatcagat aattttgttt
     8881 ccggcagcca gctgtccaaa aaagaagctt cgattgccgc ttgcggagag tctagagaaa
     8941 gcagatattg gtttctcagc attgcttttg tctgagacag ttccaactct gttatttcac
     9001 catttcgcaa actttctaat tgctcgtgaa tcagatgaag tacttgattt cggttctttt
     9061 catcgatacc ggtttggaca ctcatgaatc ctcggaacgt atccacgcta ctagaggcat
     9121 aataagctag actttctttc tctctgacat tcatgaaaag tttagagtga ggaaaaccgc
     9181 caaataaacc gttaaagacc attagcgcaa aacgttctgg ttcatcgtaa tacacatttg
     9241 tctgataagc taggttcagc tttgcttgaa cgattggctc acgtacttgt cgttccttca
     9301 cgatattcac ttgaggttgg gtataaaaca gatctggata aactgtttcc cgcgactcaa
     9361 atggtaattg cccaactgct tcagctgctt tgtccggatc aacatcgccg acaacaaaaa
     9421 tgtcgatttg atcatttgcc atcatttctt gatagtagga cgctagcgaa ctagcagtga
     9481 tcttgtccaa atcttcgact acgcccaacc ctgggatctt ttgttccggg ctgttttcaa
     9541 aataaagttc ttgcaaggca taggaagcaa aagtttgttt atcttccttc aaactttcca
     9601 aatatagacg cagattgttc ttttccaagt caaacgtcaa ctgatcaaac tgatgatttt
     9661 taatatttgg ataaaataaa acctctttta aaaaatctac cgcttgagaa aaaaggtttg
     9721 gatcattgac atattcacca ttcacaaaac tgatccctgc gtttagccaa tgtaaatttc
     9781 ctttttttct aactgataat ccaaagcttg cgccataaag ctctgcaagt tgcgcactta
     9841 actttgtctg atccgaataa ttcaaactat ttgtttccaa catgctgctg agaagtgaac
     9901 gtttcgtgat gacttcttgt tgcagacgcg cagtaaaacg gataaaaatc cggatcgttt
     9961 tgtattgttt tgttggtaag acatgaagat tcacgccttc tgccaattga tacaccatat
    10021 ttatatgctc cttttcacag ttgattgccc gcttactctt ttaatgggtc aacctgcctt
    10081 ttttacttat tcctctgctt gaaatttata agttcaggca attcgccgtt ttttctaaat
    10141 gatgttacac tttatagtat agcatagatg ttttttatca aaagaaacag acggctaaaa
    10201 gcgtatattg tttttttatt ttttattttt cgatatacta aagacaaata aaagaaggga
    10261 gggtcattca tgatcaaaga attcaaagag ttcatcatgc gtggagatgt cttggatctt
    10321 gctgtcggcg ttgttattgg tagtgcgttt acaagcatcg taaaccaagt ggtgcaaggc
    10381 ttgatcacac cactaatagg ttggtttgtc gcaatgatca caggtacttc tgatttagaa
    10441 ggagcacttt caattcttga ctggtcgccg acaaaaggcg tcacatttgc gtttggtaac
    10501 gttattagtg ctatcattac atttctgatc actggttttg tcttattctt agttgtcaaa
    10561 gcggcaaacc gtgcgaagaa tttacgctcc aaagaagagc aagtggaagt ggaagaaact
    10621 cctactgcag aagattatct tagtgacata cgcgatcttc ttgccaaaca agtggcagaa
    10681 gacaatacac atccagtaga gcaaacaaat ctttccgata cttctaaata ataaagaatc
    10741 taaaaaaaag aaagccgatt ttcagtgtcg gctttctttt tttagattct taacttgcta
    10801 cttgttcctt tttcatgtcg atatgtggaa tatcatcttc caaatacaca tctgagattg
    10861 ccttgaatcc aaacgactca taaaaagctt gtaggtattc ttgtgcgccg atcacgattg
    10921 gtttttctgg aaaatttttc tggatcatgt taatcgtttc ttgaacaatc tttcttccta
    10981 aaccatcttt acggttgtcc tgatgaacaa ggacacgtcc aaaatggata ctctccgctt
    11041 cttcatatac tcgcgtgtag gcaaggatct tcccatcagt atcttgaaaa aacgtatgcc
    11101 aagcttctcc atcaatgtca tcaacttctt gataaggaca ctgttgttcc acaacaaaaa
    11161 ctgcgattcg ttctttgacg atcgcgtaaa actctgctgt tgacaattct tcaaatttct
    11221 ttacctgata ttccactaag caaacgcccc ctgctttttt attcttagca taccgattcg
    11281 cacacaaaaa agcaaacgaa ccttacgaat ctcaggctcg tttgcttttt aaactgcttc
    11341 agttaaaaat tcgatctcaa ctgtccgagt aagtacatct gaatagctgt aagatactcg
    11401 ttcaaaagaa ttttcttcag gatccaaatc aacaacgaat actgaaggat aggtttctgt
    11461 taaaacgccg cggcgttctg tttgacgctt tcttcctgtc tgagcaacca acatgattgg
    11521 gctgccgata cgaccttcta aatctttctt gattgaagct aaagttgttg gcatacagtt
    11581 cacctctact atcaactata ccacatattt ttaaaatttt caagtttacc ataaaaaaaa
    11641 aagaaatgca ataaaaataa ataagaagct cattaagatt tttctaaatt attcctcatt
    11701 ataaaatttc aagtgttttt ttattttgag ttttactctc ccgtaagcgc tcattacttg
    11761 cttgtgatcc attgctaaac attcagccat gtctacaaaa tcaacgcctt gcatataata
    11821 atgaaaaatt ttagcttcta tttttgaaaa ctggatttcg caattttcga ttgcttcttt
    11881 taataaaaga tgctgaattg ctaaaggttc ttgcaatgtg atctctgcta aaaagtctgg
    11941 tccttctgtc tctatttttt ggtcgattga gacttcatct ttttgatcac gacgcttcag
    12001 cgcgttctgc ttcctcaaca gagaatagat ttgattgctt ataacgcttt tataataact
    12061 tccaaagcta acacctttgc ttttttcgta agttctcaat gcctgatata aagcgatcct
    12121 tgcttcttgt tcccaatcct cagaaacata atttcttaaa taataggtgt ttttcaaacg
    12181 tagtaaaagt ggaaaatatt tttgatacag ggcagcaaat gcttcttcgt caccattacg
    12241 tgcagcgcgc aataactcct cagacataaa ataattcctc cataaattcg tttatggagg
    12301 aatcttatca tcaatgattc atcgaactga ttagaacgat atgttattta ttttttctca
    12361 ttaaatcgtc caatttttgg gacaattttt ttaactgctc ttcattccat ggagaattac
    12421 gtcggaaatc aataaaatgc atatctttat tttcttgggc tattgttttt tccgtttttt
    12481 ttatcgtctt atataattca taagcagaag ttcgtaatgc tccttgggaa aagatcatcc
    12541 actgttctgc taaatcgctt gtcgccactg tcacttgtgt caaacggtca ttcagctgac
    12601 cagcgattct ctcgatataa ctgtcagccg tttcgtcttc tttcgtaaaa acaacatcca
    12661 actggtattt cttataattt tgttggatgc ccggaactag ttgggcatcg aagacaacga
    12721 tgacttccat tccttcatat ttcgcataat ttgataaacg atgtaaaaga gcttctcttg
    12781 catccgccat tttgtcctgc tttttaagct ggacaagttc cggccacgaa ccaatcatgt
    12841 tgtagccgtc tacaatcaac agctgttttt tcatggtcta ctccccctga cgttgggaaa
    12901 aagcctgata cataagcaaa ccagcagcaa cactggcatt taagctctgg acgtgcccgg
    12961 tcatcgggat cgtcagcaat tcatccattt cttttttcaa tcctggactc attcctttgc
    13021 cttcattacc aatgactaaa gcaattgatc ctttggcatt ccatttttgg aagtttgtgc
    13081 cggtcatatc tgtgccaaat acccaaaatc ctgtttcttt gagctgttgc acagcttgta
    13141 ccaaattcgt cacgcgtgca acagggacat gttcgactgc cccagtggaa gtctttgtta
    13201 cgataggtgt gattccaact gctcggtgtt ttggtatgat cacaccatcg acgccacttg
    13261 cgtctgctgt ccgtaaaatc gacccaaaat tgtgtggatc ttcgatgcta tccaaaatca
    13321 aaaagaaggg gtcttctttt ttctcttttg ttcgttcaat cagttggtca agggataaat
    13381 attcataagg tgtaatcgca agaacgactc cttgatgcac gccgtgatcg ctcaaagttt
    13441 ctaatttttg tttaggaacc catttgactg gcacagcttg ttcacgtgcg atttctttca
    13501 gttcttctac tttttctcct cgtgaatctt cttgaagaaa gagtttattc cctcgccctt
    13561 gttgtagcgc ttctactgcg gcatggtgcc caaagacgaa attatcttct aatgcttctt
    13621 cattttgatc agaaactgac tgatttcgtc cagtttgctt tttatttgaa tatctatttt
    13681 gttttttgtc ttcaaatcca cgtttcgatt gatgtttgtt tgttctttct ttacccattt
    13741 ttttcaccaa ctttctgaat acaccagcgg atcaattctt ccatccgctc ggtttgtttt
    13801 gtcaaatgca gatagcccat gagcgcttcg aaccctgttg cgacccgata cgtcgtaaca
    13861 tcagcatttt ttgcgctcgt atggctttta gcatttcttc ctctgcgata ataaagttct
    13921 tcttcttctg tcaaaagatg ttcttccagc atttgctgaa tcaaaaaggc ttgtgcattc
    13981 gcagaaacat aattcgttgc cgcacgatgc agcttattgg gttttgtttc tccttgatca
    14041 atcaggtggt cacgaacata gatttcgtaa atcgcatctc caacataagc caaggctaaa
    14101 ccgttcaatt gtgtgtaatc tctcatgctt ctcttctcca tcgggttcct tgcggtgtat
    14161 cttctaataa gatccctttt tctttcaaca aatcacgaat ttcgtcgctt cggacaaaat
    14221 cacgattgct tcttgcttga tttcgttctt cgatcaattg ttcaatctct tcatctaata
    14281 actcttctgc tgtaaataaa ataccgaaga tcgaaagcca ttgcgagaat aaagcttctc
    14341 cttgtgccaa gacagcggca gaaactttcg gctggtctag ataagtgttc agccacttag
    14401 caagctcata gaccactgtg atcccatttg ctgcattaaa atcatcgtcc atttcttcga
    14461 tgaatcgctg ttctaaattt tctaacaaag cgattttatc cgcatcatct gccaaattct
    14521 ctgaggcagt ttcttggcgg aatttcaaat tgtcaaacgt attcttcagt cgttgtaaat
    14581 tggtggctgc ttctttcatc gtcgtttcac tgtagcggat cggacgacgg tattgtgtgg
    14641 tcgccatgaa gaaacgcaag acttgtggat caacgttttt gatcatttca tgtacagtga
    14701 tgaaattccc tagagattta ctcatttttt catcgtcttc gccaatcgtt acatagccat
    14761 tgtgcatcca gtaattagcg aattttttcc ctgttttcgc ttcactttgt gcgatttcat
    14821 tttcatgatg aggaaattct aaatcttgcc cgccaccatg gatatcgatc gtttctccta
    14881 aatgttttgt tgccataacg gaacattcaa tatgccaacc gggacgtcct ttgccccatg
    14941 gtgaatccca agaaatctcg ccaggttttg cttgtttcca taaagcgaaa tctaatgggt
    15001 cttctttttg ctgctgttct tcccctgtac gctgacttgc tccgacttcc agttcatcga
    15061 tggattggtc acttaatttc ccataatttt caaatttacg ggtacggtaa tacacatcac
    15121 ctgaagattc ataagcatag cctttttcaa ccaacacttg aataaatgct aaaatatctg
    15181 gcatatgatc catgactctt ggatgcagtg tagcaggttc gacattcaac gctttcgtat
    15241 cttcttcaaa tgctcggatg aaacgatcag caacttctgg tgctgtaata cctaattcgt
    15301 ttgctgtacg gatgatttta tcgtctacat ccgtgaaatt cgatacgaaa tcgactttat
    15361 agcctcgata ttcaaaatag cgtcgaattg tatcgaaagc aatggtgcta cgagcatttc
    15421 cgatatggat ataattatac acagtcggtc cgcagacata cataccgaca tgtccctttt
    15481 gtatagggat gaattcttct ttttttctgg tcaatgtatt gtagattttt atcattctga
    15541 tctcctcact ttttctcctt tgattcgaac gacttttgct ggtactccta ccgctgtagc
    15601 gtcgtctgga acatctttca acacaacggc acctgcaccg attttggcgt tttttccgat
    15661 cgttaccggt cctaatatct gcgcattcgc agagagcatg gctccttgtt taacagttgg
    15721 gtgtctttta cccgtttctt ttcctgtgcc gcctaatgtt acgccatgga ataaaatcac
    15781 atcgtcctcg atttctgcgg tttcgccaat gacgattccc attccatggt cgatgaacac
    15841 gccagttcca atcgttgctc ctggatggat ctcaacacca gttaggaaac gccagaattg
    15901 agcattgatc ttcgccaata aaaagagccg gtgctgatat aaaaaatgag agaaacgatg
    15961 ccaaaacaag gcatgaaaac cagggtatgt caaaacgatc tcagctgtcg aacgtgcagc
    16021 cggatcgttt ctctttgctg catcaatcgc tcgggtaaac caactcatga ctttccactc
    16081 ctttcgtaag aattttatgt aggaaatcat cttcttataa agaaaagaag cgccttttaa
    16141 ataagtttcc ttatcaaaag acgctttccg cgtggttcca cttttcttca taatcaatga
    16201 ttatctctga cagctaacgc ctgttatacg ttcttggcta ctttgtttcc tataaaacag
    16261 tttttcctgt tttctgtgtt tcgccaagac cactcccaga ggcatttcgg caaccattca
    16321 ttgatacctt gcaccacccg gtatctctct tgtaatgatc agctgcttac ttttttctgt
    16381 tcatcgtgtt tagttcagta cttgatttaa atgtgctaat gatttttcac gaccaagcaa
    16441 ttcgatcgtt tcaggcaatt ctggaccatg catttgtcct gatacagcta cacggattgg
    16501 catgaaaaga ttttttcctt ttacgccagt ttctttttga acagctttga tggcagcttt
    16561 gacagttgga gcatctacta cttccatttg ttctagctgt gctttgaatg cgtggagaac
    16621 agtaggaact gtttcaccag caagtacttc ttttgctgct tcattcaata caggatgttc
    16681 attgaaaaat agcgtagaca gatcaacgat ttctgcagca tagctcattt gaggttgata
    16741 caggctaacg atcttcttca cccattcgat tgtcttttca tctgggtttt ctgctacacg
    16801 tccatcagcg attagataag gcagacacat gtcagtaaat gcatctagat ccattgcttt
    16861 gatgtaatgg ttgtttaccc attgcagttt tttcgcatcg aaagctgcgg gtgatttact
    16921 taggcgtttt ggatcgaaca ttttgatcaa ttcttggtgt tcgaagattt cgtcttcgcc
    16981 cacaggtgac cagcccaata atgcaataaa gttgaacatt gcatctggta gatagcctaa
    17041 ttcgcgatat tgttcgataa attgcaagat tgattcgtca cgtttgctta gttttttacc
    17101 tgtttcactg ttgatgatca atgtcatatg tccgaaggtt ggtggtgtcc agccaaatgc
    17161 ttcatagatc atcagttgtt ttggtgtatt ggcaatatgg tcatctccac gtaaaacgtg
    17221 agtgattttc atcaaatgat catcgaccgc aacagcaaaa ttataagttg gcataccatc
    17281 gcgtttttgg atgacaaagt cgccaccgat attatcggat tcgaacgaaa tagcgccttt
    17341 caccatatct gtaaatgcgt attctgtatt gcgaggcaca cggaaacgta caacagattc
    17401 tagaccttgt gcttcttttt ctgcttgttc ttcaggtgtc aagttcgcac atgttcctgc
    17461 gtaatgaggc atttctcctc ttgcgcgttg tgcttcccgt tctgcttcta attcttcttc
    17521 tgtgcaatag catttgtatg ctcgattgct agcaagcagc tgatcaatca gcggttgata
    17581 gatttcttta cgttcagatt gacggtaagg gccgtattct ccaggatttt caggagattc
    17641 atcccaattc attcccaacc aagcaaggtt ttccaactgg cttttttcgc catcttcgat
    17701 attacgtttt tggtcagtat cctcaatacg aataataaag tctccatcat tgtggcgggc
    17761 aaacaaataa ttgaataatg cagttcttgc atttccaata tgcaggtgcc cagttggact
    17821 tggtgcatag cgtacgcgta ctttcgtcat tttcttcctc ttctttcaaa ctagactttg
    17881 actaacagca aaaattccgt cagttacggc ttctcaaata tcatttattt aaagccactc
    17941 agcacaaatt ttaccttttc taaggtaaat agtaaagcca actggttgaa tttccttacc
    18001 agttggctga atttcttttt tattttgatt gtttagtctt tttctctgtc tcttttttat
    18061 tgtcgattcc tcgtcctgca tgtgcaggtt tagcaaagat catgcgtcca gcagctgttt
    18121 gaagggcact tgttacaacg acttcgatac gttcattcat gtaatgctgt ccatcttcta
    18181 cgacaaccat cgtgccgtca tcaaggtagg ctactccttg ttggcgttct gtcccggctt
    18241 ttactactag aacattcatc gtttcaccag ggatgacgac tggttttacg gcatttgcca
    18301 acgcattgat attaagaaca ggtacatttt ggaattcaga gactttattc aagttatagt
    18361 cattggttac aatgacacca tctaaaagct tagccaattt gatcaatttg ctgtctactt
    18421 cactgatatc ttcaaagtct ccatcgtaca tttcgacaga tataccctct tctttttgca
    18481 aggcatttag gatatctaat ccgcgacgac cacgcacacg tttcaaacta tcccctgagt
    18541 ccgcgatgta ttgtagttcg taaagaacaa aattagggat catcaaggtt ccttctaaga
    18601 atccagtctt tgtaatatca taaatccgac cgtcaatgat cacgcttgta tccaagattt
    18661 tatatttatg gaaattttct cctgattttc gttctaacag ttgactctca acttgactag
    18721 ctgtttcttg ctcgattttt ttgcttcgtg aaccaaaaac tttcttccat tcttcgattc
    18781 gtgtcgttcc cattctcaat cccagatagc ctaagatgat catcagtaaa atcggcaata
    18841 cactattgac gaatggaatt tccatattgt aaagaggggt cgagatcaca acacctaaag
    18901 tcaatccgat gatcgcaccg atcgcaccga agagaagata agtcaagctg acctcactta
    18961 gtttttgttc tacttgtttg ataatagctg agatatgttt ggccaacagc aaagataata
    19021 aaaagaaaat aagtgcgcct aacaggctgt tggtaaatgt attattcagc catccgtttg
    19081 actgttgacc gattgcctgc caagctgagg gtaaaaatga aatacccaag cttgcgccga
    19141 caatgatcat cagcaaagta atgacacgtt tttgcatcgt ttttcctccg tttcgacaat
    19201 catttattag ttagcggaac acacgcttca acgtttcagc caaagttgct actccaacta
    19261 tctcgattcc ttttggcgct tcccagttac ctaagttgtt tttaggtagg tatacttttg
    19321 tgaagccgag tttttggact tctttgactc gttgttcaat atgactgaca cgtctgattt
    19381 ctccagtcag tccaatttca ccgatgaaac attccgttgg ctgtgttcct ttttctttat
    19441 agcttgaagc aatactgaca gcgatagcta aatcaatcgc tggttcattt aattttaccc
    19501 cacctgcagc ttttaagtaa gcatcttgat tctgaagaag caacccagct cttttttcca
    19561 gcactgccat gatcagagat acccgattga aatcaagacc tgttgttgtt cgtttggcat
    19621 ttccgaacat agtaggtgtg actaatgctt ggatctctac caaaattgga cgtgtccctt
    19681 ccattgccac aacgatagca gagccagttg caccgtctag gcgttcttct aagaaaatct
    19741 gtgaaggatt agcgacttct tccaatccat gctcatgcat ttcaaaaatc ccaatctcat
    19801 tcgttgagcc aaatcggttt tttactgctc gcaaaatccg aaaactatga tgttgttcac
    19861 cttcaaaata taacaccgta tccaccatat gttctaacat tcttgggccg gcgatcgagc
    19921 cttctttggt aacatgaccg acgataaaaa tggctatccc atttgtttta gcggttttga
    19981 gtagttctgc agtggtttct cttacttggc tgacacttcc tgccacactt gtgatgtctg
    20041 gctgcgtcat cgtttggatg gaatcaatga taacataatc aggggagata tgttcgatgg
    20101 cacgactgat ttcgttcata tctgtttcag cgtatagata aaattctgtg ttgatacttc
    20161 cgaggcgttc cgcgcgtaat tttatctgct ctgcgctttc ttcgccggat acatataaga
    20221 cttttccgcc tatggctgct aattgctgag aaacttgtaa cagcaaagtg gatttcccga
    20281 tacctggatc gccaccaatc aagaccatag accctggcac cacacctccg cctaacacac
    20341 ggttcagttc ttctaatttt gttttgatcc gaggttcttt tttagggacg acgtctgcga
    20401 tttttgttgg ttttgctttt tctcctgtca aacttgtccg agttctgcgg tccgttg
//
LOCUS       DACDYI010000061        19811 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.061, whole genome shotgun sequence.
ACCESSION   DACDYI010000061 DACDYI010000000
VERSION     DACDYI010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.061"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            251..526
                     /locus_tag="IWM52_002097"
     CDS             251..526
                     /locus_tag="IWM52_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974328.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            539..2611
                     /locus_tag="IWM52_002098"
     CDS             539..2611
                     /locus_tag="IWM52_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP9974329.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2776..4662
                     /locus_tag="IWM52_002099"
     CDS             2776..4662
                     /locus_tag="IWM52_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9974330.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQEAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4674..5621
                     /locus_tag="IWM52_002100"
     CDS             4674..5621
                     /locus_tag="IWM52_002100"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP9974331.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5641..6168
                     /locus_tag="IWM52_002101"
     CDS             5641..6168
                     /locus_tag="IWM52_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9974332.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6231..6884)
                     /locus_tag="IWM52_002102"
     CDS             complement(6231..6884)
                     /locus_tag="IWM52_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP9974333.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7016..7858
                     /locus_tag="IWM52_002103"
     CDS             7016..7858
                     /locus_tag="IWM52_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9974334.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8016..8912
                     /locus_tag="IWM52_002104"
     CDS             8016..8912
                     /locus_tag="IWM52_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP9974335.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8915..9628
                     /locus_tag="IWM52_002105"
     CDS             8915..9628
                     /locus_tag="IWM52_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP9974336.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9648..10166
                     /gene="msrA"
                     /locus_tag="IWM52_002106"
     CDS             9648..10166
                     /gene="msrA"
                     /locus_tag="IWM52_002106"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP9974337.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10163..10390
                     /locus_tag="IWM52_002107"
     CDS             10163..10390
                     /locus_tag="IWM52_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP9974338.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10542..11093
                     /gene="lepB"
                     /locus_tag="IWM52_002108"
     CDS             10542..11093
                     /gene="lepB"
                     /locus_tag="IWM52_002108"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP9974339.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11176..11718)
                     /locus_tag="IWM52_002109"
     CDS             complement(11176..11718)
                     /locus_tag="IWM52_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP9974340.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11955..12353)
                     /locus_tag="IWM52_002110"
     CDS             complement(11955..12353)
                     /locus_tag="IWM52_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP9974341.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12613..13473
                     /gene="ylqF"
                     /locus_tag="IWM52_002111"
     CDS             12613..13473
                     /gene="ylqF"
                     /locus_tag="IWM52_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAP9974342.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13466..14233
                     /locus_tag="IWM52_002112"
     CDS             13466..14233
                     /locus_tag="IWM52_002112"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP9974343.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14289..15146
                     /gene="dprA"
                     /locus_tag="IWM52_002113"
     CDS             14289..15146
                     /gene="dprA"
                     /locus_tag="IWM52_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP9974344.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15268..17346
                     /gene="topA"
                     /locus_tag="IWM52_002114"
     CDS             15268..17346
                     /gene="topA"
                     /locus_tag="IWM52_002114"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP9974345.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17315..18691
                     /gene="trmFO"
                     /locus_tag="IWM52_002115"
     CDS             17315..18691
                     /gene="trmFO"
                     /locus_tag="IWM52_002115"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAP9974346.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            18889..19794
                     /gene="xerC"
                     /locus_tag="IWM52_002116"
     CDS             18889..19794
                     /gene="xerC"
                     /locus_tag="IWM52_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP9974347.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT         6874 a         3413 c         4120 g         5404 t
ORIGIN      
        1 tcgcagcccc atttttttgt tgtgataaaa gaacaagaaa ggcaggaatg ggaaagtaaa
       61 catccttttg tcagaaactt ttatctttct taagttttac ttgatatctg atactatttg
      121 gaatagaatg aggcgttgaa aaatttatgg aaatttctaa ctagcaataa acgatgaaac
      181 aatgaaatat ttttcatcca aaaagaactt tgctataatg atggaagttt gtgtatagga
      241 gatctggaaa atgagagaga gttttgaaca acaaaaaaaa ctgttgcatg atcgctatgg
      301 cgccctttcg atggatgacc gccgtcaaat actttgtaag ttgcgtaaaa gaaatatatt
      361 gatgtatcgt cagttggaac gtctgaaaca tgatctgctt cgtttggagt caaaacgtgt
      421 acagtgtgaa ttagagggga accaaacaca ggttgaagta gtcgaaacca aaattttaaa
      481 gaaaaaagaa caatttttaa aaatgcttac acaaaataaa aaataaaggg gatggacgat
      541 ggaatttgct gagttagtta ttgtatttgc agtaacgatc accttatcga acatagctag
      601 ccgcattttg ccaatgattc cagctccact gatccaaatt ttccttggag tggtattagg
      661 tctgacagag tggggacaat caatcgattt tgaacctgaa ttgttcttag ttatgattat
      721 tgcaccattg ttatttagag aaggagaaaa agcagatatt tcttcgattc tcaaaaattt
      781 cgggacaatt ttgttcttgg catttggagg agtcattttg acccttgtcg gggtaggtgc
      841 gacgttatca ttcctgttgc caagtgtccc acttgctgcc tgctttgctt ttggagcagc
      901 gttaggtccg acagatgcgg tagcagtcag ttctctttct ggacgagtga atatccctaa
      961 aaaggcgatg catattttgg aaggagaagg tcttttgaat gatgcatctg gtgttactgc
     1021 cttccagttt gctcttggtg cgctgatcac cggatctttt tcagcagtaa acgcggggat
     1081 gagtctagta gtttctagta ttgggggagc tttgatcggc tttttgcttg tctggttcaa
     1141 acaaaaaata attcatttga tcgaaaaagc atcagcacaa gatgtcacag ggtatctgct
     1201 gatcgaactt ttgcttcctt ttctagccta tgtattggca gaggcatttg atgtatctgg
     1261 aatcattgct gcagtggcag caggtattct ccaagcttct ggttttagga aaatctctgt
     1321 gtttgatgca gaattgtcga gcttgtccca cagtacatgg acaactattg cttttacact
     1381 gaatgcatta gtatttatat ttttaggaat cgaattgacg caagtgttct cgccagtatg
     1441 gggagacggt ttatatccaa acggtctttt attagcgatt atcgtattaa tctctgtcat
     1501 gctttttgtg atcagattca tttctatcag tcttttttat gtattcaaag atggcagtaa
     1561 aaagttcaaa aaacagttga acgagatctt gatcttaaca tttggcggtg taaagggaac
     1621 agtcagtttg gctacgattt ttattttgcc attttcaatc aataatatga tgttttacca
     1681 gcggtctttg cttttgtttc tgacggcggg tgtcatctta gtgactttgg tcatagggat
     1741 cattgtcttg cctatgctga cggaaacaga ggaagcgaaa agcacggatt tgaatgcttt
     1801 gatgatttta gaagaggtcg ttgaagtttt gcgaaaagaa ataaaggaaa ttgatcgtaa
     1861 tacaaaagaa tttttagcca ctgaagcagt gatagaaaat tatcaagaac ggatacgtga
     1921 tctgtacttg gaagatttaa ccgatgatga aaagcaagaa gtacaagaaa tacaagcact
     1981 gattttgtcg atcgaacggg atggattgga tgaaagttat cgttcgggca agctttcttc
     2041 taatggttat cgtttttact cccgttttct ttctcgattc gaacattcta tcacaagcca
     2101 gattttgtct tttatcggct tttggttcat tgttgtccgt aggttaatgc gaatcgttct
     2161 ccatccgaaa atgttttggc aaagaagata tgctgatcgt cagacattta ttagtgatga
     2221 agatatccaa gaaatcagag agacatatca aaaaaatact cagttgatta ttgaaagttt
     2281 agataacttg actgatattt atgatgaaac gatggttcaa ttttttatcc agcaacgcaa
     2341 acttgaagga ataaaaatga actcggggaa tttgatttct tcgtggatga tccagcaaga
     2401 tagtctgttt acgaaaaaaa tgctaagagg ttattatctt gaaagaaaaa tcattgatga
     2461 atacgaagtt gctgaaaaaa tcacgacgtt ttctgcgaac agctatcgca ggaatatcaa
     2521 tttgttagaa tcttatacga tgaacaaacc atctgataac ttttctttcc gatttgcgtt
     2581 tcgagcaaag tcaaaaaaac gagtccaata aaataaaaag gtaaaaattt tcaagtttta
     2641 taaaaaacaa aacatatcag aacaggatcg aatcggtcct gttctttttt tgagaacaca
     2701 aaaagaacaa ataccaaaaa aattttcctg aagttcacta tttttatgat acaatatcca
     2761 agataaggag ggaacatgtt gaaagaatta aaagtaactg acctcaaaaa aacttatggt
     2821 gaaaaagatt tgtttgacca gatttctttt ttggtccacg aaaaagaccg tatcgggttg
     2881 attgggacga acgggacggg taaaacaagt ctcttaaata ttattgccgg tatcgatagc
     2941 agcgacggtg atcggcaaac agtgttttat ccaactgatt accgtattgg ttatctttct
     3001 caagaagctg aattttccga tgaattaact gttctccaag cagtctttca aggaaacagc
     3061 cccttgatcc aaactgttcg agcatatgag gaagcattga tcgaactagg ggaaaacgga
     3121 gaagaccctg atgtgcaaaa acgttatgct aaagcagaag aacaaatgaa taaagaagat
     3181 gcttggacta cagataccaa cgcaaagatt attttgcaaa aattaggaat cagtgaatta
     3241 gataaaaaaa tcagtacttt atcaggtggt cagaaaaaac gagttagtct ggcgcaagtg
     3301 ttgattgatg aacccgattt actattacta gacgaaccaa caaaccattt agattatgaa
     3361 gcgatcgagt ggttggaaaa ctatttgaaa caataccgtg gcgcactatt gatggttacc
     3421 cacgatcgtt attttctaga tagagtagca aaccgaatct tcgaactttc atttggaaaa
     3481 ttatacgagt ataaaggaaa ttatgaagct tacgtcttag aaaaagcaga acgtgatcgt
     3541 gtagaagtag aacaagaaga aaagcgcaag cggctttata aacaggaatt agcttggatg
     3601 agggctggcg tacaggcacg tggcaccaag cagcaagcac ggatcaaccg tttcgaagat
     3661 ctaaaggaga acttgtacca agtaaaccaa gaagatgatt tagagctgaa cctagcgaca
     3721 caacgtttag ggaaaaaagt actggaaatc aaagatggca gttaccgtat aaatgaccaa
     3781 acactgctag aacatttaga tttattgatt caatctcggg aaagactagg tattactggt
     3841 aaaaatggtg cgggaaaatc gacattgctt aatattctag ctggcagaat tcccctggat
     3901 agcggtacaa tgtctatcgg tgaaacggtt catcttgcgt attatacaca agaaaatgaa
     3961 gaaatggcac cagataaacg gatgattgct tacttacaag aagctgctga agaagcaaaa
     4021 acagcagatg gcagtcaaat cggagtagca gaattacttg aacgattttt atttccaaga
     4081 tttatgcatg ggacactgat aggcaaactt tcaggcggag aaaaacgccg gttatttttg
     4141 ttgaaactgc tgattcagca acctaatgtt ttattattgg atgaaccgac caatgatttg
     4201 gacatagcaa cattgacgat tttggaagat tattttagaa gttttccagg tgctgtgatc
     4261 actgtttccc atgaccgtta tttcttggac aaggtagcag ataagttatt agtattccaa
     4321 ggaaacggaa aacaagaact ttattacgga aacatgtcca gttatttact gaagcaaaaa
     4381 gagacccagc agcctgctga aaaagcgaaa ccaaaaatac agagcaagga acctgctgaa
     4441 aagaagaaat tatcttacat ggaacaaaaa gaatgggaaa cgattgaaga tgagattgca
     4501 gaacttgaag aaaaaatctc attgctccaa gaagagatga atcatcaagg agataatttc
     4561 actcgattac aagaattgca aaacgacgta tctgaaacag aagcacaact tgaagaaaaa
     4621 atggcacggt gggaatactt gagtgaatgg gtagaagatt aggaggaaaa aggatggaac
     4681 aagcgtatct tgacttagga agaaaattgt tggaagaagg gcatcgtaaa gaagacagaa
     4741 caggcacagg cacattaagc ttattcggat accaaatgcg gtttgatctg caaaaagggt
     4801 ttcctttgtt aacaacgaaa cgagtacctt ttggattgat caaaagcgaa ttgctttggt
     4861 ttttaaaagg agatacgaat atccgctatc tattacaaaa taaaaaccat atttgggatg
     4921 aatgggcatt tgaacgctac atcaaaagtc cagactataa tggaccagat atgactgatt
     4981 ttggccatag atgtttgaca gatcctgaat tcaatgaaga atataaaaag cagagcaagc
     5041 ttttttgcga gaagatccta accgatgatt cctttgctga aacatacggc gatcttggac
     5101 acatctacgg ctatcaatgg cgtcactggg aaacaaaaga cggtggcttt atcgatcaaa
     5161 tcaaacaagt gatcgaagca atcaaaaaga ctcctgattc tagaagattg atcgtttctg
     5221 catggaatcc ggaagatgtt cctagcatgg ctttgcctcc ttgtcatacg atgtttcaat
     5281 tttatgttca agagggacgg ctaagctgcc aattgtatca acgcagtggg gatgtgtttc
     5341 taggcgttcc gttcaacatt gcaagttatg cgttgctgac tcacctaatt gcacatgaaa
     5401 caggtttagc ggttggcgaa tttgttcaca cattaggaga tgctcatctt tatatcaatc
     5461 atatcgaaca aatgaaagaa caattgagcc gagaaatacg cgcatttccg actttagttt
     5521 tgaatcagga aaagcagtca gtatttgatt ttgagatgga agatatccaa ttagaaggat
     5581 atgacccaca tccggcgatc aaagctccta tcgctgttta gaatcagaaa ggatgagaaa
     5641 atgttagctg caatttgggc acaggatgaa aacggattga ttggaaaaga agaccgtctt
     5701 ccttggcgtt tgccaaatga tctgaaattc ttcaaacaaa tgacagaagc aaatacacta
     5761 gtgatgggac gaaaaacatt cgaagggatg ggtagccgtc cgttgccgaa tcgtcagacg
     5821 attgttttga cgagagattc ttcctataaa gcagaaggcg tgcatgtcat gcatagtgtg
     5881 gaagacgtgc ttgctctgga aaaagaaaca gacggtatct ttttcatcgc tggagggtca
     5941 gctgtttatc aagaatttct gccattttgt acgatattat accgtacagt catccatcat
     6001 gtattcgatg gagatgctta ttttccacct gttgactggt ctgattggtc actgatcaat
     6061 actagtcaag gagaaatgga tgaaaaaaat cgttatccgc atcaatttga aacgtatcag
     6121 cgtaatgaga atcctgtaaa aagcgggaca ggcgaattga cgatataaaa aagctatgga
     6181 tagactagct gttatttaga gctgtcccat ccatagcttt atttttttta ttaagtaaag
     6241 aataaaacag agaagtacat aaaacctgca cctaacataa caaacaaatg ccaaacaaca
     6301 tgcatatagc ggatagaatt caagctataa aagaatgcgc ctatcgtgta agaaagcccg
     6361 cctgctacca tcaaaataac accagtcatt ccaagagagt gataaagagg aacagcagcc
     6421 acgacacaga gccaccccat aaaaatatag atgatcgtag atacttttga tactctctcc
     6481 cgtttatgca acgtcaacgc tttatatata atgccaagaa tcgcaaacaa ccagattaat
     6541 acaaataaag tccaacctag ccatcctcgg atactcagca aacaaaaagg agtataactt
     6601 cctgcaatca aaagaaaaat cgaactatga tcgaatactt ggaacacctt ttttgctttt
     6661 gtaaaaatca atgaatgaaa caaagtagac gttaaaaata aaagaatcat catcgatcca
     6721 tagatagcat aagagacaat atggagggag gaatgcaagt gggcaccttt tactaaaaga
     6781 accactagtc ccgcgatact caatccagtc ccaattccat gggtgactgc attaaacact
     6841 tcatttaata tttcatacgt tcgattaaat ttcgtttttt ccatgaaaac ggctccttta
     6901 ctaggtgtaa ttaatgaaaa atcattattt gtctgttata ctaaaaatag aaattgtctt
     6961 acatgtagtt taacatgaat aaggcatctt gttaaagaaa gagagagaga aaataatgac
     7021 aaatgtaaaa atcgtaacag attcttcatg tacgatggaa aaaaatgtac gcgatgcttt
     7081 aggcattcgt gtcatgccgc tatccatcat gatcgacgga acagtatatc ccgatgatga
     7141 ccaactagac ggcgaaagat ttatggaaat gatggcgaaa gcaaaaaatc tgcccaaaac
     7201 aagccaacca ccaattggag aattcatctc gatgtatgat gagctaggag aagacggcag
     7261 tgaagtgatt tcgatccata tgacaaaagg cttgagtgga acagtagaag cagcaagaca
     7321 agccagtaat ctaacaaaaa cgaaagtaac ggttatcgac agtgatacca ctgaccaagg
     7381 attatctttt caggtaattc gagcagctga attagcaaaa gaaggtaaaa cagtagagaa
     7441 aatccttgaa gaagtcaaaa aagtacaaga caatacaaaa ttatacatcg gaatatccac
     7501 actagataac ttggtaaaag gtggaagaat cagccgaaca actggtttgc tgtctagtgt
     7561 tttgaacata cgcgtggtga tgaatttcgc ccattctgaa ttaatccctg taactaaggg
     7621 gcgaggaaag aaaacgttcg ataaatggtt taataatttg aaaaatgaac tgaaagaatt
     7681 gccaaatgtg cgacaaatcg gtatttcaca tgcggatgca aaagaattgg cgcaagaatt
     7741 caaagagggt ttgcaagaat tattcccgga catgcatatc cctgtactgc atacgaatcc
     7801 ggttatcgct acgcatactg gtaaagacgc gtttgcaatc atgtattatg tagattagtc
     7861 gaaaagagga taagccctca gcctgaaagg cttgcagctt attctctttt tctttataaa
     7921 attaggaaaa taattgtttt cttttttcat aaagactacc tttttgataa aatttttgat
     7981 aacattagaa ccaatcgaat acaagaggtg aaaaaatgaa attaacgaaa cgacatttga
     8041 tcctcgtttt gttcccatta ttaattgcga tcatcatcta tgcaggttta gctgcttcta
     8101 taccgaaagc tggcccacta atgaaaccag taactactgt gagagaagat ccaaataaaa
     8161 aagaaaaact tcactacata gctatcggag attcgctgac tgaaggagta ggagatcaaa
     8221 ccaagcaagg aggattcgtt ccccttgtcg caaatgatct aaaagaccgt tataatttga
     8281 ccgcggttga aacagaaaac tatggtgtaa atggtgaacg aagtgaccag atcttaaagc
     8341 gagtaaaaac aaaagaagaa attcgaaaca atatcaaaac agctgatttt atcacactga
     8401 ctgttggcgg taatgatttg atgaaagtta tccaaaataa tttattcggg ttaacggtca
     8461 attcctttaa aaagccactg aaaaactatc aggaaaatgt cacagaacta ttggaagaat
     8521 ttcgatccct caataaaacc gcacctattt acattttagg catctacaat ccattttatt
     8581 tgaactttcc tgagatcact gacatgcaga caattgttaa caattggaac aaaggaacag
     8641 aaacggtgac aaaaaaaatg ccgaactgct actttatccc aatcaatgat ttgctttatc
     8701 aaggattgaa tcaggaagta ggtgtcacag aagaaggaac agatcaaaca actagcacga
     8761 cagaaaatct aaatggcatc aaaaataatg tgctctatga agaagatcat ttccatccaa
     8821 ataatttagg ttatcaattg atggcaaatg cagtgagaga tcagctgatc gagacacaaa
     8881 accaatggtt agagaaggag gatgatcaat aaacatgaaa acaagatctg acgtatcttc
     8941 aaacaaaaca aagaaaacaa attactggaa attcgccttc cttatccttc ttggggtagt
     9001 acttggttcg accgtatttt taggcagccg tatttttgct aatcgggaac cagaactccc
     9061 agaaatacca gctcttacgg aaagacaagg agatcctgtc ttaacgatca attcaaataa
     9121 agaaaaagtc aatcaaatca tcagcttttt ccttagtgag tatcaaaaag atagtgatat
     9181 tgaatataaa ttttatttgg aaaatgaagc actactcaat ggtactttcg aagtactagg
     9241 ttttcctatc gatttctatt tatattttga tccgtatgtg atggaaaacg gaaatgtcca
     9301 actaaaagct agaagtcttt ctatcggaac attgagtttg ccaatcaaag atgtgatgaa
     9361 tatgatcaag agaaactata aattaccaga ttggatcgaa gtcaatacag atgatctgac
     9421 tatcatgctt aggttggatc aattccgaat gcaaaacggg atgtatatta aagcagataa
     9481 aatcgactta gtaaatgatg acattcggtt tagtctatat ttgccaaaag atacctctga
     9541 tgcttcaaac aactcaaata ccaacaaaac tagcaccagc agtactgaaa gcaagacaaa
     9601 agaaaataaa aaatcatcgg atgagtagta ttagaggggg aatcataatg gaacgagcaa
     9661 tatttgcagg aggctgcttc tggtgtatga tccagccttt cgacacgctt cctggtatcc
     9721 atacagtgat gtcaggatat actggagggc atgtaccaga tccgacttat gaacaagtaa
     9781 aaagccaaac gactggacat acagaagcag tggagattat ctatgatcca acattgattt
     9841 catatgaaca attagtagaa ctttactggc agcaaactga tccaactgac gctttcggac
     9901 aattcgagga tcgcggtgac aattatcgcc cggtgatctt ttatttgaca gaagaacagc
     9961 ggatcattgc tgaaaaaagt aagaaacagt tagctgaaag tggacgtttc gaacagccaa
    10021 tcgttacaaa aatcgaacca gcccaaccat tttatctagc agaagaagac catcaagagt
    10081 attataaaaa gcatcctgaa aattttaaaa gaaaccatgc acgccgggct gcctttatcg
    10141 aagaaaattg gaggaacgaa gggtgaatcg aagctttttt cattatttaa tgacactgcg
    10201 aggcggtggc acacatgatg ctttatctgt tttcgctaca gaagccggta aagacatcca
    10261 atttcctaag cacagctcta gctacgaaga aatttctaat tatctggaac tgaatgtcga
    10321 ctatttgccg tccatggata tctttgacga agcatgggaa aaatatttag aaaataatca
    10381 tgacatataa gagatagttt tgtactttat gatcaaaaaa gttttttgtc caatagccta
    10441 attgaaagaa ttatttttat ttcagctatc tagtcgcatt tttttgatat ttttcttata
    10501 ataaagaaag aatgaaaaaa agagggatgg attgatagag attgacaaaa aaacaacgct
    10561 atattaatcg cttctggctt gtattcaaat acttacttgt ttctgtattc cttgcgttca
    10621 tgttgcgggg atttttattc atcccggtac cagtggaagg caattcaatg gaaaacgtgt
    10681 tgaagcaagg tgatatggtt gtgatggaaa aattttcgga gatcagacga tttgatattg
    10741 ttgtttttca attagcagat ggcacgattt atatcaaacg agtaatcggt cttccaggag
    10801 aaaatgtcag ctaccaaaat gatcaattga agatcaatgg gaaagtagtt aaggaacctt
    10861 atctaactaa aaacctgaaa tcagatcatg caaatgcatc ctatacgacg gactttacac
    10921 tacaagagct aacgggacaa tccaagcttc cggaagataa ttattttgta cttggagata
    10981 atcgaagagt ttcaaaagat agtcgttcgt ttggcacgat caataaaact gatattctag
    11041 gtaaagcaag atttgtctat tatccacttg atgaaataaa atggatccag tgagaaacaa
    11101 taaagcgtgc agtctgttga gactgcacgc tttattgttt aatccaaaat cagtttacta
    11161 ccttagtacg taaaatcaaa cagctcgttt aaacactttt ctagcctcta aagttttgat
    11221 aaatgaataa acaaccatta tttgaatagg gatttggatc atattcctga tgaatctacc
    11281 tagaaaaatt actttgaaat ctgcaccaaa caaaacagac atcagccaag aattgataaa
    11341 aaggctgact gggatcataa tcaataattg ggcagcgatg acggaaggca ggtttatctt
    11401 tttatgatat aaaaataagc cattaataaa tccaccaagg aaagccgtga taagaaaagc
    11461 agcttgaaac gttcctgaac caaatatcgt gaaatttaaa gtgttcgaca acatttcaag
    11521 caaaccagcc cagatcggtc catatagata acccataagc gccatactta aaaatccgaa
    11581 tgtgatttga aaataagcag gaccaatcgc tatcttgtat aagatgacat caagggctaa
    11641 taaaagagca gaggtcgaca acaatcttgc attcatttcg ggcaatctaa aaacgtcttt
    11701 tgcaatcaaa gtgttcaaaa tattctctcc tttgtcagtt aataataaaa ggataccata
    11761 aaatcaccaa tgtgtcatga cacattggtg atttacatac actggcatct aacaattaaa
    11821 aaaagggtga gaagactgct tttataaact gtaaaaaaga gattgtacga ataaaaaaaa
    11881 tagaggcgga gaatacttat cgtatcatcc gcctttgttt ttttgttata aaaaacgtca
    11941 gattagttat ctgcttacat cttcatccat acgggttcta atgtatttcc gtctagatct
    12001 tggacttcta atgaaaacat ttcttcttca ggcattccca tatctacttc gtggaaagta
    12061 ccaccatttt cttttgcaat ttgagcaaat ttttttactt catccgcgtt ttcaacacta
    12121 aacgaaatca aagcaccact tgttttttta ttatcagcta tttctttatc ttttataaat
    12181 tttttataaa aatcatgaga gagaagcatt atccaaaaag tgtcatccca taccattgaa
    12241 ccagcttctt cagtagaaaa ttcttcattt tttttaaaac ccagcttttc ataaaaagca
    12301 acggaacgat tgacatcagc aacaggaaaa ttaacaaaaa ccatactatt catttgtttc
    12361 gtctcctttc tgttttgttc gtctatcatc tcttatttag gaaacgctgt caaaaattta
    12421 gattcatata tagaaaccaa aataaaaatt tgcatcatca aaaaagtgaa gatgtgatag
    12481 aatgaggact ggttcacttt cacttaacta atagatggaa tttctttata ataaaaaagt
    12541 gaatttatat tgaaaaagaa aaaaacaagt aaagccgggg aatattccgg tatgaaagga
    12601 atggagagaa caatgaccat ccaatggttt ccgggacata tggcaaaagc ccgacgagaa
    12661 gtttctgaaa aaatgaaata tgtggacatc gtatttgaat tggtagacgc acgattacct
    12721 ttatcttctc gtaatccaat gctagatcag atcattcagc aaaaacctcg ccttgtttta
    12781 ttgaataaag cggatctggc agatccacaa cagacaaagc tgtggcaaga atattttcgt
    12841 aaacaaggcc atcttgcatt agctatcacg gcacaagaaa gtaaaggaat taaaggtcta
    12901 gtacctgcgg ctaaagaagc attgaaagaa aaacgggcaa gagatgctgc tcgggggatc
    12961 aagcctcgcg ctattcgggc aatggtctta ggtattccaa atgttgggaa atctacgttg
    13021 atgaatcgac tagtaggcaa gaaaattgca cagacaggaa ataagcctgg cgtaacaaaa
    13081 ggtcagcagt ggcttcgttt aggtaatgag ttggaattgt tagacacacc agggatttta
    13141 tggcccaaat ttgaagatga agaaatcggc aaaaaactag cgctgacagg cgcgatcaaa
    13201 gaccagcttg tccatttaga tgatttagcg atttacggat tagacttttt tgcccgatat
    13261 tattcaggaa gaatcagcga tcgttatggt ttatcaatcg agcaagaaga aaaattggca
    13321 ccggaattac tgatggatat tacagcaaaa cgtgggtata aagaagacta tgatcgtggc
    13381 agcgaattag tcgtgcatga tattcgtcaa ggaaaacttg gacgatacac actggatcgt
    13441 tgcgaagaaa tggagcaaaa aagtgatgac tgaatccatc caaagtatca aagaacgatt
    13501 gaaaagcata cagagaatag atgatcctac actagaatca ttaagaaatg atccacgcaa
    13561 aggcgtgcag caagcactgg cttcttttga acgacgattg aaaaaggaag cattaattaa
    13621 ggaaaaatat gaagaaatgt tttgctttga aaagcaaggg tacgcacaag gtcaccaatg
    13681 gattgccggc attgatgaag taggaagagg accattagct ggacctgtcg tggcagctgc
    13741 agttatcttg ccgaaaaatg agatcatatt agggctgaat gactccaaac aattatcaga
    13801 gaaaaagcga gaacttcttt atcaacaagt tcaagaaaaa gccctggcaa tcgggatcgg
    13861 gatcgttgat cagcagacaa tcgatgatgt gaatatttat gaagcaagta aattagcgat
    13921 gatccaagca gtagaaaaat tgtccattgt accaagctat ttgttgatcg atgcgatgac
    13981 cttggatttg cctattcagc aggaaaaaat aatcaaaggg gatgcacgat ctgtgtcgat
    14041 tgctgctgca agtatcattg ctaaagtata tcgagaccaa ttgatgaaag aatatgacgt
    14101 gctttatcct ggatatggtt tctcgcataa tgcaggttat ggaacaaagg aacacttaga
    14161 aggacttgaa cgacaaggaa ttacgccaat ccatcgtaaa agttttgcac ctgtaaaaaa
    14221 atatttacaa taaaaaacag ccgcttttac gtatcttatt agaaagagag acgaaaggcg
    14281 gttttatgat gtatcaaata gaagaaaatt tattgaaact tgcttataca aaaggaatca
    14341 gtagtcaagg aaaatgggca cttgctaact ggatgatgca gtatccttat aaagaaatcg
    14401 gatttgaaga catcattaag ataggtaaaa taaccaaaca tcgggagctg tttcgcaaaa
    14461 gttggcagga aattcacgct aactggggca aaatccaatc gaaacagtcg tttatcactt
    14521 gctttgatcc caaatatccc cctcagctgc ttcacttgac ctatccgcca attgtactgt
    14581 tttataatgg tgatctatct ttactttctt gtaacatgtt aagcgttgtt ggtggaagac
    14641 acacttctgc tttagccaaa aaaacagttt attatttact aaaaccagtg atagaagctg
    14701 gatatgtgat cgtcagtggc tgcgcgaaag ggatagatac atacggtcac caagcagcaa
    14761 tctctcatgg tggacggaca atcgcagtca ttggaacagg gatagagcaa gtctatcctg
    14821 ctgaaaatcg tgacttgcaa gaggtgattg caaatgatca tcttcttttg agtgaatatc
    14881 ctcctcaatc cggcccgaaa aagcattgtt ttccaatgag aaatcggatt atcgcagctt
    14941 tgtctaaagg aacatgtgtc tttgaagcca agaaaaaaag cggctctctg ataacagcac
    15001 agcaagcatt ggatcttgga agaacagttt tttctgttcc aggaaatatt ttaacagaac
    15061 attctaccgg atgtcatcat ttgatccaag acggtgcagt ttgcacggtt tcgggtgaag
    15121 atatcttggc agaatttaaa gaataacacc atacttttca tttgcttttc ttgacaaacg
    15181 ttttctgaat ggatatgatt agctacgatt tgtatgaatc ctcttttaat agtaggaaca
    15241 cgacaatgga aaggagtcca ttcagtaatg gcgtataaat atttagtgat tgtagaatca
    15301 cctgcaaaag ccaagacaat tgagaaatat cttggacgaa actataaagt aatggccagt
    15361 gttggccata taagagattt accgaaaagt aaaatgggta tcgattttga aaacaattat
    15421 gaaccccatt atatttctat acgcggaaaa ggcgatgtca tcaaaagcct gaaagccgca
    15481 gcaaaaaaag ctcaaaaagt ttacttggca agtgacccgg atagagaagg agaagcgatt
    15541 gcttggcatt tagcgtacct acttgggttg gatctgaaag aaaaaaatcg ggtggtcttc
    15601 aatgaaatca caaaagacgc agtcaaagca gcttttaagg aaccaagaac gatcgatgta
    15661 gatttagtag atgcacagca agctcgtcgt accttagaca gaatcgttgg ttattcgatc
    15721 agtcctattc tctggcgtaa ggtcaagaaa gggttaagtg caggacgtgt ccaatctgtc
    15781 gctttaaaaa ttattattga ccgtgaaaaa gagatccgag aatttgttcc agaagaatat
    15841 tggagcatcg acggtaattt taaaaaagct cgcaagaaat tcaaagcaaa tttctgggga
    15901 atcgacggta agaaaaagaa attaccagat gcacaaagtg taaaagaagt cactgctaga
    15961 attgatggaa cagacttcga agttaaaaaa gtagaaaaga aagagcgcaa aagaaaccct
    16021 gcgaatcctt tcactacaag tagcctgcaa caagaagctg cccgtaagtt aaactttcga
    16081 acaagaaaaa ccatgatggt agcacagcaa ttatatgaag gaatcacttt gggtaaacaa
    16141 ggaacagtcg gcttgattac atacatgcgt actgattcta cccggattgc cgatacagca
    16201 aaagcagagg ccgcagcatt tattgaagat acgtatggaa aagaattttc tagacatgat
    16261 caccgaaaga tcaaaaatgc tcaaggcgca caagatgccc atgaagctgt gcgtccaacg
    16321 agtgtgcttc gtacaccaga tgaactcaaa caatatttag ataaagatca attgaaactt
    16381 tatacattga tctggtcacg ttttgtggct agtcagatga caccagctat tttagataca
    16441 atgaaagtga cattagaaca aaatggtgtc atcttcattg cgaacggttc aaaaatcaaa
    16501 ttcaaaggat tcatgcaagt gtatgtggaa ggtagagatg acggcaaaga agagaaagaa
    16561 aatattcttc cagaacttga agaaggcgat gtagtccaat ctgttgatat tgaaccaaaa
    16621 caacatttta ctcaaccacc agcacgtttt agtgaggcta cgttgattcg tgcactagaa
    16681 gaaaacgggg tagggcgacc ttccacttat gcaccaacac ttgatacgat ccaaagacgc
    16741 tactatgtaa aattgacaca aaaaagattt gagccgactg aactaggaga aattgtcaat
    16801 tcattaatct gtgaattctt ccctcaaatc gtcgatatcc atttcacagc agaaatggaa
    16861 ggagatttgg ataaaattga agaaggcacg gaagcgtggg tcaaagtggt tgatcgtttc
    16921 tataaaccgt ttgaaaaaga actgacgaat gcggaagaaa aaatcgaaaa aattcaaatc
    16981 aaagatgaac cagcaggatt cgattgtgat gtctgtggac atccgatggt gataaaatta
    17041 gggaaatatg ggaagtttta tgcttgcagt aattttcctg attgtcggaa tacgaaacca
    17101 atcgtaaaag aaatcggtgt gacttgccca gtatgtcatg aaggacaagt cattgaacga
    17161 aaatctaaga aaaacagact attctatggt tgttcccgtt tcccagaatg tgaattcact
    17221 tcttgggata aaccagtggg cagaaattgt ccaaaatgtg acggatatct ggtcgagaag
    17281 aaagtcaaag gcggtaaaca agtagtctgt gtaaatggag attacgaaga agacatccaa
    17341 aaataataga aaagaggttc tttcattgaa aatggtaaat gttgttggag cagggcttgc
    17401 cggaagtgaa gctgcttggc agatcgcaca agcaggagtc cctgtccgtt tatatgaaat
    17461 gcgtccagtc aaatcgactg aggcgcatca cacaagtaat tttgctgaac tagtatgttc
    17521 caattcatta agaggaaaca gtttggctaa tgcagtaggg ttattgaaag aagaaatgcg
    17581 ccgattgaat tcggtagtaa tcacttcggc agatgaaaca gcagtgccag ctggtggtgc
    17641 cttagctgtt gaccgcgatt ctttttctga aacaattacc cgaaaagtca aagagcatcc
    17701 attagtaaca gtgataaacg aagaaattac tgaaataccg gaaggaatca ccgtagtagc
    17761 aacaggacct ttgacttctc atccattagc agaaagcatc aaagcattca atggttcaga
    17821 tggtttttat ttctatgatg cagctgcacc aatcgtagac aaagctacga tcgatatgga
    17881 taaagtatat ttgaagtccc gctatgacaa aggagaagca gcttacttga actgtccgat
    17941 gacagaagag gaattcaaag ctttccatga ggcactaatc aatgccgaag tggcagaact
    18001 taaatctttt gagaaagaaa aatattttga aggatgtatg ccaattgaag taatggcaca
    18061 gcgaggattc aaaacgatgc ttttcggacc tatgaaacca gttggattgg aagatcctaa
    18121 aactggcaaa cgtccttatg ctgttatcca gctgcgtcaa gacaatgctg cagcttcttt
    18181 atacaacatc gtgggctttc agactcactt gaaatggggc gagcaaaaac gtgttttccg
    18241 gatgattcct gggttagaaa atgctgaatt cgttcgctat ggtgtgatgc accgaaacag
    18301 ctttatgaat tcgccagaat tgctgaaacc gacttaccaa tcaacgaaac gcgaagatct
    18361 gttctttgca ggtcagatga ctggtgtaga aggctatgtt gaaagtgcag cgagcggact
    18421 acttgctgga atcaacgccg cacgcttagc aaaagaaatg gaaccagtcg agtttccaca
    18481 agaaacagct attggaagta tggcttacta cattactcat gcggaaggaa aacatttcca
    18541 accgatgaat gccaactatg gtctgtttcc tgaactgcca gagcgcattc gagacaaaaa
    18601 agcaagatat gaagcaattg cacaacgagc tttaaaagct aatgaggcaa taaaaacaga
    18661 attagaaaaa actgcaatcc caaatgagta aggaaactcc atgaagaggg tgagacggat
    18721 agtctcgctc tttttctggc gtttttctta agaatttctc aaacaaaatg tgaggaattg
    18781 cgccaattgt gaacaaactt agaaatattg gttaaattct gacaatttat ttgtcttttt
    18841 ataaacgctg tgctaaatta ttctctggga caaaaggaag gtgagaaaat gacagaagcc
    18901 acgtgggaac aaaaattttt gcgttatttg attgtcgaaa gaggatattc agaaaaaacg
    18961 aaagaagcat atgaagaaga cattctccat ttcaaacatt ttttggagga atctggagac
    19021 gctgatctgc tgaaggcgga atattttacg gttcgtgtct atttgagtgc attagctgat
    19081 catcgtccta gctatagcag aaattctatc agtcgcaaga tagccagcct acgttcgttt
    19141 tatcagtatc ttctcaaaga agaagtgata aaagaaaatc cattttctta cgttcatctc
    19201 aaaaagaaaa atttgagact gccacgtttt ttttatgaaa atgaaatgca agcacttttt
    19261 gacagcgtag caggagatag tcctttggat ttgaggaacc gcgctttact ggaagtcctt
    19321 tacgggtcag gcatccgttt aagtgagtgt agcagtttgt cggtatcaga cattgatttt
    19381 gattcagagg tcatgttgat ccatggtaaa gggaacaaag agagatatgc tcctctaggt
    19441 tcttttgctc aagatgctct gcaagaatat ttcagggaag gccgccaggt attgatgaca
    19501 aaatatcacg aagagcatga tatcgttttt gtgaatcatc atggaaaacc aatcacgcct
    19561 actggaattg aatatgtctt gaatcaaatg atcaaaaaaa gcagtttgga tagtgatatc
    19621 catcctcaca tgctgcgcca tacatttgct actcatttat taaataatgg ggcagatttg
    19681 cggacagtcc aagaattgct aggacatgcc aatttatcga ctacccagat ctatgcccat
    19741 gtaacaaaag aaagtttaca aaaaaactat cgttctttcc atccaagagc gtaaaaataa
    19801 ggaaaagaac a
//
LOCUS       DACDYI010000062        19456 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.062, whole genome shotgun sequence.
ACCESSION   DACDYI010000062 DACDYI010000000
VERSION     DACDYI010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.062"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            132..3659
                     /locus_tag="IWM52_002117"
     CDS             132..3659
                     /locus_tag="IWM52_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP9974348.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3656..7378
                     /gene="addA"
                     /locus_tag="IWM52_002118"
     CDS             3656..7378
                     /gene="addA"
                     /locus_tag="IWM52_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAP9974349.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7447..7788
                     /locus_tag="IWM52_002119"
     CDS             7447..7788
                     /locus_tag="IWM52_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9974350.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8097..9140
                     /gene="pheS"
                     /locus_tag="IWM52_002120"
     CDS             8097..9140
                     /gene="pheS"
                     /locus_tag="IWM52_002120"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAP9974351.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9145..11565
                     /locus_tag="IWM52_002121"
     CDS             9145..11565
                     /locus_tag="IWM52_002121"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAP9974352.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11735..12745)
                     /gene="trpS"
                     /locus_tag="IWM52_002122"
     CDS             complement(11735..12745)
                     /gene="trpS"
                     /locus_tag="IWM52_002122"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAP9974353.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12999..13736
                     /locus_tag="IWM52_002123"
     CDS             12999..13736
                     /locus_tag="IWM52_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9974354.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13750..14565
                     /locus_tag="IWM52_002124"
     CDS             13750..14565
                     /locus_tag="IWM52_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP9974355.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14578..15225
                     /locus_tag="IWM52_002125"
     CDS             14578..15225
                     /locus_tag="IWM52_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9974356.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15241..15897
                     /locus_tag="IWM52_002126"
     CDS             15241..15897
                     /locus_tag="IWM52_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9974357.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16059..16880
                     /gene="racE"
                     /locus_tag="IWM52_002127"
     CDS             16059..16880
                     /gene="racE"
                     /locus_tag="IWM52_002127"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAP9974358.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16883..18238
                     /gene="rph"
                     /locus_tag="IWM52_002128"
     CDS             16883..18238
                     /gene="rph"
                     /locus_tag="IWM52_002128"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAP9974359.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18239..18757
                     /locus_tag="IWM52_002129"
     CDS             18239..18757
                     /locus_tag="IWM52_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP9974360.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18864..19364
                     /locus_tag="IWM52_002130"
     CDS             18864..19364
                     /locus_tag="IWM52_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9974361.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6541 a         3341 c         4178 g         5396 t
ORIGIN      
        1 ttttgcgtat acttaaaaaa gtagaaatat cagaacctta aaaggatata atttgaagaa
       61 tatagaacaa atgttcagta ttttttgata taataaagga gcaccgtatc atgtgttgaa
      121 ggaggaaaag catgtcttta caatttattc ttggaaacgg gaaaaaagat caccgtcaag
      181 tcttgatcga cgaagcatcc gcttggctcg accaggatgc aagtaaccaa gtatttttcc
      241 ttgttcccaa ctacagcaaa ttcgaacagg aacaagaaat tttagcggag atgcgaagaa
      301 gaagcggaaa aaaaactttc agcacaacaa acgttcaagt atttagtttt tatcgtctgg
      361 cttggtattt gctgcaacag acaactcttc ttacaggaaa tgaattatca gaatcaggat
      421 ctgcaatgat cttgcgacag attttagaaa aaaatgagga agaattaaca atctttcgcg
      481 gagaaatcaa taaaagaggg tttattcgtc aattacaaga actctacagt gaattgcgta
      541 caggaaatat tcaaccagaa gacctgatgc ttttatttgg tgattcacct aatgcagaag
      601 accagcaact taaaatgaaa gacttgaagc tgattttttc agcttatgac agcgaattga
      661 ctcaacgtgc actaacaaat gaagatgcac tatctatact agcaaattat atgcagacac
      721 aagatttcaa caacgtcaaa tttattgtca gtggtttttc tcgaatcaat ggtcaagagt
      781 atcaattact gcaggcaatg atggaaaagg ggcaattggt tgtcgattta ttgctggatc
      841 gtccttatac agcggatctt ccagaagtac ttacgctctt tcatgagact ggaaagcttt
      901 atttccgtct ttttcaaagt gcaagagaac ggaaagtccc tatattagtg gaccgatttg
      961 caccagagca gactcgtccg acagagcttc aactgctgca aacaatgtgg gaagagacat
     1021 ctagccaaaa gaaagcggca gtacacaaaa tagggggaca agagcatctc catttgtggg
     1081 aagctgaaaa tccaactgaa gaagtccgac aattagctgt tgaaatcaga cgactagtca
     1141 gtgaagaaaa ctaccgttat cgtgatatcc aagtgctgac aaaaaatatg tctttatatg
     1201 gaaatatcct tcggccgatc tttcaagaaa tggcaattcc tttttatatc gatgaagaac
     1261 aaatgatgga aaatcatccg ctgatcgaat ggatcaacag tctgttcgct cttgaccgct
     1321 attcttatcg gctaagtgat attatgcgtt ttttcaaaac tgaattattt ctgtcgacgg
     1381 aagcagaaac cgaacttgat gaatggaaac aagagcgaga tgaattcagg aaaaaactgg
     1441 acatcactga aaatgtagca cttgcttaca attatcaagg aacttattgg actcgtgaaa
     1501 aagactggca gtttgtggcg tatgattttg aagcagaaca gattgaagac gtacagtatt
     1561 tggaagaaca gtcaaatacg attagacgat tatttcagaa agctgtacca gtatttttga
     1621 aaaaaataaa gaaaagcaaa acaggacaag aggccgcaaa tgttttttat caatttttga
     1681 tccaaagtgg cgtacagcaa caattattgt tttggcgtaa tcaggaagta gacagaggaa
     1741 acttagaagc ggcaagaaac catgagcaga catggggagc gctgattgat ctgttggatg
     1801 aatatgtagt aatttatggt gaatcggctt ttgactggtc actgttccaa gaaatcatta
     1861 caagtggtct agaaaatcta agttatggta aaatccctac cgcaatcgac caagtgcgaa
     1921 tcaatcgttt ggaattagtt cgaccaaatc aagctgccgt cacttttgca ttagggttga
     1981 atgatcaagt gtttccagac cgaaaagaga caaaaggatt gttgtccagt gaagaacgag
     2041 aagtattaaa tcaggccttg ccagatggac agtttttatt cgacccatca aaggaaagta
     2101 tctcttttga gcctttccaa gcctatcttg tttttctttc cggaacagat cgattatatc
     2161 ttagttatgc acaaagctat gacacagatg gttcgctgaa gatgtctcct tatttgcgta
     2221 gaatcacaga atatttatct gttcccgtcg agagaaaaga gcatttgacg atcgaaagcg
     2281 accctaattg ttatgtaggg acttacagaa gcggaatcaa tacagtcaat aggatttatc
     2341 gattagcttt agatgaaaaa aaacctgtac caaagcactg gcaaaagctc aaagaattag
     2401 tactgaattc gtcatggcga aattttgctt tacagatttt tgaaagtcag gaacatttaa
     2461 atgttcctgt ttcgatgcca gaaaaaatgg cggaagagct ttatggaaaa gagatttata
     2521 cttcaatttc acgtatggag aatttttata attgtgaata caaatacttc atgcaatttg
     2581 gtttgaaatt gaaagaacga actgtttatg gtctaactcc agcagcaact ggtgattttt
     2641 atcatgaatc attggatcgt ttcttcaagc tactgttttc aaaccaatta tccttagtga
     2701 atatgtccga tgcgcagcgt agagacttta ctgaaaaagt gctgcaggaa gtattcggag
     2761 aacttcggtt tgagatattg gatagttctg caagaatgaa ctatatccgc taccagttag
     2821 gacagacgat tcaaaaagtg acatgggctt tgcaaaaaca gagcaaaaat acaggcatgc
     2881 atcctcttca gacagagatt ctatttggtc agattgctgg agcaaaaggt atcacaggtc
     2941 tggaattacc gttgagtaat ggtggaaaac tccatgttcg agggaaaatc gatcgaatcg
     3001 atgtagcttc tgaacaagaa gatacttggc taagcgtcgt tgattacaaa tctagcggac
     3061 gttcattcga tgtcacagaa gcttattatg gcatggctat gcaattattg acgtatctgg
     3121 acgtagcatt gatggatgcg gtacgtttga ctggaaaggc ggctgttaaa ccagcaggat
     3181 catattattt acatgttcat aatcctgtat tgacggagac agatgatgta gagaagaata
     3241 cattgaaaaa atttagttat gatggcgttt ttgtcgatga tcccgctctt ttagaagtac
     3301 tagatcattc actggaagca aaagaacaat cactgatcta tccagtgaaa aaaaatgcga
     3361 aagatgaata ccaaaaaggt agtttcagta aagacaaatt tttcacggaa gaagaactgc
     3421 aactattcat ggcgcataat cgggaaaata tgcggttagc tggagatgcg attacttccg
     3481 gagaaatcaa actaaatcca gcttataaag ataaacagcg gattgcctgc gaattttgtc
     3541 cattcagaag catctgtacg tttgatgtga tgctgaaaga aaataattac caccgtctgg
     3601 agaaaataga taaggaagaa gcactgcgta gaatattgaa aaggagtgaa gaagagtgaa
     3661 tcaaattcct ttaaaaccgc aaaatgaacg attcacagat gatcagtggc aagcgatttt
     3721 tgatcaagga gataatctat tagtctctgc gtctgccgga tcaggtaaaa ctacagtgct
     3781 tgttcgaaga gtgattgaaa aactaaaaat gggttttgat atcgatgaat tattgattgt
     3841 gacatttact gaagctgccg caagagaaat gaaagaaaga attcaagagg cattacaaga
     3901 atccgtcaat agcgaaagtg accctgtacg tagacagcat tttactaagc agttagtttt
     3961 attgcccaca gcgaatatct caaccttaca tgctttttgc ctgacagtta ttcgacgtta
     4021 ttattactta attgatattg atccagtctt tcgcatgtta acagatgaaa cagaaacgat
     4081 cttgatgaag gaggatgtgt gggacgagct gagagaagca ctttatgcag aaaatgatga
     4141 acgatttttc caattaacaa tgaatttttc taatgatcgt tcagatgacg gcttgacgaa
     4201 tctggtgttt tctctttatg agtttgcccg tgccaaccct gacccccaaa aatggctaga
     4261 acagttaagc gataattatc gtttgcctga ggggctagcg aaatctcgat tatatcaaaa
     4321 acaaatccgt cctttagttt tggcagatat ataccaatgt gtacagttat atgaacagat
     4381 gactcagtta gcacaaggtg aaggattaga aaaaatgcat gagcaagtag ctggagaaca
     4441 acagcagatc aagaatatct atgaagcgtt ctcgcaagat cggttagaag aagcttatgc
     4501 tggtttggaa caactaactt tttcaacttt taagagcagt cggaaagcag aattgaaaga
     4561 aatttccaat gaagtcaagg gcatgaggga taaagcaaaa aaactgattc agcagataag
     4621 taaaagttat tttcctgttt ccccaagcca aatggaagaa ttaacggaca aagcactacc
     4681 gttagtcgaa gaaatgacaa aagtcacgca aagcttcatg gacggtttca gtatgcgtaa
     4741 acgggaaaaa ggtgtgctgg atttcaatga tctggaacat ttagcgttgc agattctcac
     4801 agaaaaaacg aaagatgtat ggctgccaag tgaagcttca aaacattatc gaaaaaaatt
     4861 caaagaagta atggtagatg aatatcaaga cgtgaaccaa ttacaagaag ccattttata
     4921 ctggctaaga gaacccgatg atacaaaagg aaatatgttc atggttgggg atgtcaaaca
     4981 atccatctat tctttccgtt tagcagatcc tagtttattt atcgggaaat acgagaattt
     5041 ctcaaagaaa gaaggcggac gtcgaatcgt tttggctgag aacttccgct ctagaaaaga
     5101 agtcctttct tttacgaatc ttatttttga acaactaatg gatccagcag tgggtcaaat
     5161 caattatgac gaagcagcca aactgatcca aggattttca gactttccag agaatgaaca
     5221 gtttgagccg gaaatcatga tctatgaaaa agaacaggaa gaatcagaga ttgagattcc
     5281 gacggatgat atactggaag acaaaacaga aggcgaactt tttatgacag gactaaaaat
     5341 tcgtcaatta attgattcct cctttatgat ttatgataaa aaatcaaaaa aaagtcgtcc
     5401 gatagagtac aaagacattg ttctcttgac acctacaaag aagaacaact taacgatttt
     5461 agagattttc aaaacattgg atattccatt agagatgaat gatgctcaaa actattttca
     5521 agcgacagaa attcgcacga tgatctcgct gctacaatta atcgataatc cctatcaaga
     5581 tattccgctt gcggctgtat tgcgctcacc gatcgtgggt ctgattgaac cagaattagc
     5641 cagcattcga ttagctaacc gtgcacatac ttactatgat gctgtgcttg cttatcaagc
     5701 atcaaacgaa gatgaattag cagctaagct ggaacatttt ggcaagcaac tagagcattg
     5761 gcgagaactt gctcgccgtt cttcgatcac tgacttgtta tgggatatct attatgagac
     5821 gggatatcta gagtatgtgg tcggtctgcc agcaggggca cagagacaag ccaatcttta
     5881 tgcattggtt gatcgtgcca aagcttacga gcaaagttct ttcagaggat tataccagtt
     5941 cgtgcgtttc attgaaaaaa tgcaagaaaa agataaagac ttagcagaac cagtcatttc
     6001 aatagaagat aatgctgttc gcgttatgac gatccatgca agtaaaggct tggaattccc
     6061 agttgtattt ttgttagata tgacaaagga gttcaactta caagacttgc gcaatcggta
     6121 tgcttttgaa gaaaaacttg gagcaggaat tcgctatatg gatcctgaaa cacgagtgtt
     6181 atatgatacg ttaccgtttc aagccatcaa attggcaaag caaaacaaat tgttgtcgga
     6241 agaaatgcgt aaattgtatg taggattgac tcgggcagaa caaaagcttt tcattgtcgg
     6301 ttcctataaa aataaagagc aaatgatcca aacgtggtct gaagcagctg atcatgagga
     6361 gttagttttt gatcctgcgc tacgtttgaa gggcagaagc agcttgatga attggattgg
     6421 ttacggattg atccgtcatc ctgaaatgca gaaatactta gaggaagaaa tttcgacttc
     6481 tcttcttcaa cattcaaatg cacagttttc tatttcatgg atgaatcagc aatcgatcat
     6541 tgaacaaaga cagttgcttg ctgaaaaaga actggttaac ttagatcagc agatgaaaga
     6601 agatgaaact ttattagcag attcgttaca aaaacgtctg gcatatgaat atccttatca
     6661 agcttcttca caaaccacaa gttatcaatc tgtatctgaa atcaaacgat tattcgaaga
     6721 tccagatgat acacaagaat cacgtttgac tctggaaagc agtcagaaca aagcagcatc
     6781 ccgtcaattt cgttatacgc aagaacaact agcagaacca aaatttttgc aaaaagatcg
     6841 gcaagtgtca gcagctgctg ttggaactgc tacgcatgct ttgttacaat tactgccatt
     6901 agagatgcca actacagaga gtatccatca aaaattacag gaattggtaa ggaaacggtt
     6961 agtagacgaa aaagtcgcga aaaaagtaga tgtttcttct attatatggt tctttcaaac
     7021 agaacttggt caacagctga ttgccaacaa agaaaacgta aaacgagaac aacctttttc
     7081 catgctgctt cctgctgacg aagtatttca agactatcca aatcaagaag atgaactatt
     7141 aatccacgga atcgtcgatg gatacctaga agaaaaagat catctcaata tctatgattt
     7201 taaaacagat tttattttac atccggatga tccagcagaa atagatgcaa tcgtccaaaa
     7261 atatcaagga cagctaagac tttaccaaca agcaatgtca gaagctttga ataagccagt
     7321 agaaaatgtt ttcctaattt tgcttcgagt gaaacaaatt ataaacatta acaaataatt
     7381 ataattgagc aaacttgact tatgttttgt aaggattatg ctatactctg ggaagaggtg
     7441 agtatgatgg agctaacaca gacagaattc gttttatgcc ctaaatttga aaaaacattc
     7501 tctattttag gaaaaaaatg gaacggattg attatcgatg tactactcga aaacggtccg
     7561 caacgctttg ttgagcttgc ggcaaaaatc ccgatggtca gtgatcgagt attagtcgaa
     7621 cgcttgaagg aattagaaaa agaaggaata gtcactcgaa actttgcctg tagcgaaaag
     7681 agccgttcag aatatctctt gactgaaaaa ggagcagact tgcaaaaagc gatgcgtcaa
     7741 atacaagttt ggagcgaaaa atggatcaca gaggaagaat gcggttgatt ttgtaaaaaa
     7801 aatcttgtaa actatagtcc atacgaataa gtaaagaacg atgacgaaaa agagtagttt
     7861 ggctaaacca gttcagggag atctgtcgca gactgaaagc agatcctgtg tttgaaaaat
     7921 gaagttcact ttccgagttc gtctgataaa atcagaccgg caattgtcgt taacgaagta
     7981 agagtgcggt gtaagtcatt tatccgcaaa ttaggatggt aacgcgaaga cctcgttcct
     8041 taacaggaat gaggtctttt tgttttgtat ttggaattta aaggaggaac aagcacatgt
     8101 ctctacaagc acaattagag agcttgaaag aagcaacgct cgtcaaaatc acagcggcac
     8161 aagatttaaa tgaattaaat caaatacgtg ttgaaacgtt aggaaaaaaa ggaccgatca
     8221 cagaagtatt acgtgggatg aaagatctaa gtccagaaga gcgaccaaaa gtcggaagtt
     8281 tcgcaaatga gatccgagat cttttgacag aagagatcga gcgaaaaaaa caagtattag
     8341 aagaagaagc gttaaatgca gctttggcaa aagaaacaat agacgtgaca ctacctggta
     8401 gaaaagtaaa acatggtaca cgtcatatcg tgacacaaat catggaagaa attgaagata
     8461 tttttgtggg tatgggctat cagatcgtag aaggatatga agtagaatct gatcattaca
     8521 atttcgaacg gatgaatctg ccaaaagatc atccagcaag ggacatgcaa gatactttct
     8581 atatttcaga cgagatattg attcggacac atacttcacc agtccaagca cggacaatgg
     8641 aaaaacatga tttctctaag ggtgctctac gaatgatctc gccagggaaa gtcttccgca
     8701 gagatacaga tgatgcgacc cacagtcatc agttccatca aattgaaggg ctagttgttg
     8761 ataaaaacat cacaatgggc gaccttaaag gaacattaga agtcgtaatg aaaaaaatgt
     8821 ttggggaaga tcgtgaaatt cgtttgcgtc caagttattt cccatttacg gaaccatcag
     8881 tagaagtaga tgtcagctgt ttcaaatgtg gtggtgccgg ttgtaacgta tgtaaataca
     8941 ctggatggat cgaaatttta ggagctggca tggtacaccc gaatgtgttg aagatgtcag
     9001 gaatcgatcc agaagaatat tcaggctttg ctttcggcct tggaccagac cgcgtagcta
     9061 tgttgcggta tggagtgaat gatatccgta atttctatca aaatgactta cgtttcttaa
     9121 gtcagttcaa gggggagtaa gaaaatgtta gtttcatata aatggctcaa tcgttatgta
     9181 gatttatcga atgtaacacc aaaagaatta gcggataaga tgtctgtgac aggaatcgaa
     9241 gtagagggag taacggttcc agaagaaggc ttgaaaaaaa tcgttgtcgg tgaagtcaag
     9301 gaatgcgtac cacatccaga ttctgatcac ctttctattt gtcaagtcga tattggcgaa
     9361 gacgaactgt ctcaaatcgt ttgtggagcg cctaatgtga aggttggaat caaagtgatt
     9421 gttgcacttc ctaactcacg tatcgctggt aatgtaaaaa tcaaaaaagg aaaaatgcgt
     9481 ggccaagtat caaatggcat gatttgctca ttacaagaaa ttggctattc agatagtgtt
     9541 ataccgaaag aatacgctga aggtatctat tatatgccac aagaagctgt aaatggcgat
     9601 ccagtttttc cttatttgga tatggatgat tcgattattg aattatctat cacaccgaat
     9661 cgtgcggatg cattgtcaat gcgtggcgta gcttatgaag ttggggcaat ttatcgtcag
     9721 actcctacat tcgatgacga aaagctggta gaatcctcac gtcctgctag cgataaaatt
     9781 caagtggaag ttgaagataa agaagcagtt cctgcttatc aaatcagaat cattgaagga
     9841 gtaagaatcc agccaagtcc tcaatggctg caaaatcttt tgatgaacga aggcattcgt
     9901 ccaatcaaca atgtagtaga tgtgacgaac tatattttgc ttttattcgg acaaccacta
     9961 catgcatttg actacgacaa attgaaatca aaagagatct ttgtccgccg tgcaaaagaa
    10021 aatgaaacat tagtaacact agatggagaa tctcgggaat tgacaactga caatctcgtc
    10081 attactaacg gcaaagaacc agttgcttta gctggtgtca tgggagggtt agactcagag
    10141 atcacgaatg aaacaacaac agttgcacta gaatctgctt tatttgatcc aatttcgatt
    10201 cgtcgtacag caaaacaatt taatttgcgt agtgaatcct ctgcacgttt tgaaaaagga
    10261 atcaaccatg cgactgttgg agaagccagt gaggttgctg cagcaatgat cgtttctctt
    10321 gcgggtgggg aagttctaca aggcgcggtc aaaggctcag agatcaaagc agaagacgta
    10381 tcagtgaaga tttcacttga acgcatcaat caatatttag gaacatctct tgtcgttaaa
    10441 gaagtaaatg agatttttga agcacttgga tttagctatg aagaaaagga gtatgtttat
    10501 tccgtagtag taccaccaag acgctgggat atccaaatcg aagcagatat tatcgaagaa
    10561 gttgctcgta tttatggata cgaccagttg ccatctacac ttccaagtgg agaaacggtt
    10621 gctggaagct tgtcaaaaga acaagaaact actcgtaaaa ttcgtacaat ctttgaagga
    10681 agcggattga gcgaagcaat cagttatgca ttgacaacag aagaaaaatc aagacagttt
    10741 gttataaatg aaagtaatat tacacgtttg gactggccaa tgagcgaaga acgttctgta
    10801 ttacgaatga atttgatcag cggactatta gatgatatca gttataacac tgcccgaaaa
    10861 aatacagaga ttgctttata tgaaattggt cgggtgttct tccaagaaaa tgatccacgt
    10921 acacatctgc cgcaagaagt aaaacatgca gcaatcgcta tttctggtat ttgggaagaa
    10981 aaagattggc agacgaaagc tcaaaatgta gactttttca ccatcaaggg tcttttagaa
    11041 aacttatttg aacaactagg catcactgaa gatgtccatt atcaaacagt gacagaaatg
    11101 aaggaaatgc atcctggaag aactgctgcg atttaccttg gcgaaaaatt tattggtttt
    11161 gtcggtcaag tgcatccaac gattacaaaa agttatgata ttccagaaac atatgttgct
    11221 gagtttgatt tatctgctat agtggaagct gctcaaaaag ggatcgtttt cgaagcagtg
    11281 acaaaattcc cagcagtcag ccgggacatc gctttgcttg taaaagaaac tgtgacaaat
    11341 caggaattaa cagaagttat ccgttctgct gccggaagat ttttaacaga tatccaatta
    11401 tttgatgtct atcaaggaga aaacattgaa aaaggacata aatccatggc gtatagcctg
    11461 acattcgtca acccagaagc aacattgaca gatgaagaaa tcaataaagc aatggaaaaa
    11521 gtcgaaaaag cgttggtaga aaacctagag gcaagtattc gctaagaagg aaaatatagt
    11581 aagtaacgat caagtagctt ttgaagacca tacatttgaa aaaaagatgt gacacaactt
    11641 cgtcacatct ctttttttga ttttaaaaaa ggaatagcac agagacgaga taaaaaaaca
    11701 ggcctttaca gcctgctttt ttagagtaaa tgaattaatg gaagtaattg attcccattg
    11761 ctgattttac ttcagaaagt gtttgtgcag ctactttagc agcttcttca ctacctttac
    11821 gtaagatttc cataacagct gaaggatctt tttccaattc ttctcggcgc gcacggatcg
    11881 gtgcaaattc agcttccaat acatcaatga ggtatcgttt gatctttaca tcaccaagtc
    11941 cgccgcggcg gtaatgagct ttcatttctt caattgcttc tttatctgtt ccgaatacat
    12001 ccaagtaggt gaagaccata ttcccttcta cttgtccagg atcttgtaca tggatgtggt
    12061 ttggatcagt atacatactc atgacttttt tctgtaagac atcggcagga tcggagatat
    12121 aaataccgtt tcctaacgat ttactcattt tgccatttcc atcgattcca ggtaatctac
    12181 ccattccttt tggtgggaat acgccttttg gttccaccaa gacttcacca tatgtatgat
    12241 tgaagctttg tacgatttcc tgtgtctgct ctagcattgg tttttgatcc tcaccaacag
    12301 gtacaagatt cgctttgaat gcagtgatat ctgctgcttg agaaacagga tagataaaga
    12361 atcctgtagg cacgctttca ccgaattttt tttgttcgat ttctgttttg acagtcggat
    12421 tgcgccgaac gcgtcctacg ctgactaagt tcaaataata catggttaat tcagctaatt
    12481 ccggaatttg tgattggata aataaagtag attttgcagg atctaatccc acggctaaat
    12541 agtctaaagc aacttgcaag acatttgatg aaaccttctc aggattttta gcattatctg
    12601 tcagtgcttg catgtcagca atcatgacaa acagttggtt gtcttcatct gcctgcatag
    12661 ccactcgggt ttttagagaa ccgacgtaat gccctaaatg cagcttccca gttggtcgat
    12721 ctcccgttaa aataatattt ttcaaaaaag acacctcttt acgatttgta agtctgagca
    12781 cttaatatct taccgttttt cctgaaaaga aacaacagtt cctaaggaaa aaacaaggaa
    12841 agaggataaa ataactaata gattagaaat ggaagaaata tttatgggag aataaacgtt
    12901 ctaagaaact ttacaaatta ctaaactatc agatataatg tattttatta tacatcttta
    12961 aaagaaatct tactaaataa taatgggagt gtaattctat ggcaatgatt aaatttgatc
    13021 acgtggataa atattatggc aagttccacg cattaaaaaa tatcaatctc gaattcgaga
    13081 aaggagaagt tgtcgttgtc attggtcctt ctggttcagg aaagagcacg atgcttcgct
    13141 gtatcaatgg attagagacg atctcttcag gtaagctgct gattaatgat actgacttgc
    13201 atgataaaaa gacaaaactg acagaagtcc gaaaaaacat cggcatggtt ttccaacatt
    13261 tcaatttgta cccaaataaa actgttctgg aaaacatcaa attagcacca atcaaagtgt
    13321 tgaagcaaga ccaggcaact gcagtcaaaa acgcggagaa atttttgaaa actgtcaata
    13381 tgttagataa gaaagattct tatccttcca tgttatccgg gggacaacag caacgggtgg
    13441 caatcgccag aggtttagcg atgaatcctg agatgctgct gtttgatgaa cctacttctg
    13501 ctttggatcc ggaaatgatt ggtgatgtat tagacgttat gaaaaaattg gctcatgacg
    13561 gtatgtcaat gattgtcgtg acacatgaaa tgggctttgc caaagaagtg gcggatcgtg
    13621 ttatcttcat ggctgatgga gaagtattgg aagacagcag aaatgtgaga gactttttcg
    13681 aaaatccaaa tgaagtccga gcacagcaat ttatcagtaa agttatcaat cattgatagg
    13741 aggaaaaaga tgcgcaggtt aaaaaaaata cgcctgattt tccttttagg cattgctttt
    13801 cttgcactag ccggatgtaa aggaaatggg ttggcaaatg aagatatttt agagcgaagc
    13861 aaatctacca atgaaatcat ctggggtgtc aagtatgata ctcgattatt tggaatgatg
    13921 gatattgaga gcagaactgt acaaggattt gatgtagata ttgcaaaagc aatcactaaa
    13981 aagatccttg gtgacaatgg aaaaacagaa tttgttgaag tcacttctaa aactcggatt
    14041 ccattattga aaaacggaaa tatcgatgca attattgcaa cgatgacgat cacagatgaa
    14101 cgaaaaaaac aggttgactt ttcagatgtc tatttcgatg ctggccaagc tttgttggta
    14161 aagaaaggaa gccaaatcaa aagtgtagat gatttgaatg cttctacaac ggttctagca
    14221 gtcaaaggtt ctacatcggc tgcgaatatc cgacagcatg caccagatgc aaaaatttta
    14281 gaattagaaa actatgcaga agcatttact gctttacaat ctggtcaggg agacgcgatg
    14341 actacggata atgcgatttt gttagggatt gcagatgaaa atccagagta tgagttagtt
    14401 ggtggaacgt ttactaatga gccttacggg attgcgatca acaaagggca agaaaacttc
    14461 ttgaaagctg ttaaccaagc gctagaggaa atgcatgcag atggcacata cgataaaatc
    14521 tatcaaaaat ggtttccaaa tgaaactgaa ggaaaagtag agtaaggggg agaggcaatg
    14581 agcgaattga tacaaactta tggaccaatc tttgtcgacg ggttcaagat cacattgttt
    14641 tcgagtgtct tagcacttgt gtttagtttg attatcggaa cgttaatggc aatcttccaa
    14701 ttatcaaaga ataaagtagt gagcgcctta gcaaaggcat atgtagaatt ttttagaaac
    14761 attccattat taatcatcgt gatgtttttt tatgttgtcg taccgattta ttggtttaat
    14821 ctagatggat tccaagcagg tacgattgga ttaacgatct atacatctgc ttttattgct
    14881 gaaacagtac gttcagggat tcaaaccgta ccaaaaggtc aaacagaagc aggactatct
    14941 tctggtttta cctattcaga aatcatgcgt tatattgttt taccgcaagc attcaaaatc
    15001 gttatcccgc cattaggcaa ccaatttatc aacctcgtaa aaaactcatc tattttagca
    15061 atcgtagctg gtttagattt gatgtatcaa ggagacttga tcgctagtga aacattcaat
    15121 accttcgata cgtatatcat cgttgggtta ttttacttag ttatcacgct accgttatct
    15181 tacttaatgg tttatcttga gaaaaaatgg gcggttcgtt catagaagga ggggaaatac
    15241 atggatttta gcggcgcttt ttcatggatg aatatccgtt ttttattgga aggattaaag
    15301 gttactgttg aagtttcatt gttttcgatc atttttagtt tcttgatcgg tggtctgaca
    15361 ggtgtgattc gttttgcgaa tatccctttc ttttccaaaa tccttggtgt agtgattgat
    15421 attatacgga atcttccatt attattgatc attttcttca cttactttgc cttacctcaa
    15481 atcggcattc agatgaacat cttctggtcc gctgtcgcag cattaacgat ttttgagtca
    15541 gctatgttgt cggaaatttt ccgagctgga ctcaatgctg ttccaaaagg acagatggaa
    15601 gcaggattat caacgggact gacttatgta gaatcgatgc gaacgatcgt gttaccgcaa
    15661 gcatttaaat caatgatccc ggcaattgtg agccaattga tttcactgat caaagatact
    15721 tcattagcag taatcatttc tttacctgaa ctttctcatc aggcacgtat tatttatgga
    15781 cagaacacca attatgtgct tccgatgttc atcatcatga cacttatgta cttcgtggta
    15841 tgttacgcgc tttctctgct ttccggttac ttggaaaaga gacggtacag ttattaatag
    15901 atgattcttt tagcggttgt ggcaaactaa agtcacaacc gcttttcctc tttccattcg
    15961 aaaaaaagag atttcgattg ttttaagcaa gtaagagcac ttgaaaattc acgctaaaaa
    16021 gtacacaata gggaggatag aatttattga aaaaaaggat gatacgattg acagataatc
    16081 gccctatcgg atttattgat tcaggtgtcg gcggcttgac tgtagtaaaa gaagccctga
    16141 aacaattacc gaatgaaaat attttatttg taggagacac agcacgctgc ccatatggcc
    16201 ctagacccgc ggaacaggta atccagtata cttgggaaat gacggattat ctggtggagc
    16261 aaggaatcaa gatgctggtg atcgcctgca ataccgcaac tgcggtggct ttagaagaaa
    16321 tcaaagctgc tctttctatt ccagtcatcg gtgtgatcct tcccggtact agagcggcag
    16381 taaaaaaaac acaaaataaa caagttggca ttatcggtac gattggtacg gtaaaaagtc
    16441 aagcttatga aaaagcactg aaagagaaag taccagaatt gactgtgaca agtcttgctt
    16501 gtcccaaatt tgtttcagtt gtcgaaagta atgaatacca ttcatcggtg gcgaaaaaaa
    16561 ttgtggcaga aacattagct cctttaacca ctaaaaaaat cgatacattg attttgggat
    16621 gcacccatta tccattatta cgccccatca ttcaaaatgt aatgggagaa aatgttcaac
    16681 tgatcgattc tggagcagaa acagtaggtg aagtatctat gctgttagat tatttcaatt
    16741 tgagcaattc accgcaaaat ggtcggacat tatgccagtt ttatacaact ggctctgcca
    16801 aacttttcga ggaaatagct gaagactggc ttggaatcgg acacttaaat gtagaacata
    16861 tcgaattggg aggaaaataa ggatgcgtca tgacgggaga aaagcccaag agcttcgcaa
    16921 actggagctt caaacaaatg ttttcaaata tccagaagga tcggtagtga tccgttttgg
    16981 tgatacaaca gttatttgtt ctgcaacaat agaaaattct gttccccctt ttttgagaga
    17041 aacaggaaca ggatgggtga cagcagaata tagtatgctg cctcgagcaa cgagtacgag
    17101 aaatcgtaga gaaagcagca aagggaaact ttctggtaga acaatggaaa tccagcgtct
    17161 tattggtcgt tccttgcgtg cagtagtaga cttggaaaaa ttaggagaac gtagtatcat
    17221 cgtagactgc gatgtgatcc aagcggatgg cggtactaga acagcaagta tcactggggc
    17281 atttgtggca ttgaaattag ccattgaaaa acttttaaga gaaaaagaat tgtcagaaga
    17341 tccaataaaa gaacatcttg ctgcagttag cgtaggaatc ttaccagatg ggacttgcgt
    17401 cacagatttg gattaccaag aagattccgc agcacttgta gacatgaatc ttgtaatgac
    17461 agaatctgga aaattcgtgg agattcaagg aactggagaa gaagcaacgt ttgatggtga
    17521 acagttgaat gaaatgctct tttttggaaa aaacgcgatt gaagatttga tcaaagagca
    17581 aaaacatgct ttgctaacag agtttgctca aaatgatgag cggatagaag aaacaaaaac
    17641 aatcatcatt gcgacaagaa atccaggaaa agcagaagag tttcgaaata tgttcaaaga
    17701 agcgggatat catgtaaaaa cattacttga ctatcctgaa ttgccagatg tggaagaaac
    17761 tggcagcaca tttgaagaaa atgcccgatt aaaagcagaa acgattgcac aattgcttga
    17821 tcagccagtc ttagcagatg attcaggttt gaaagtggat gctttaggcg gtatgccagg
    17881 aatctattct gcacgttttg caggggaaca aaaaagtgat gcggggaata acgccaaatt
    17941 attatatgaa ttgacagatg tgccggatga aaaaagaaca gcacaattcc attgtacgtt
    18001 agtattcgcg gcacccaaaa aagacagcct tgtagtagaa gcggaatggc ccggtcgagt
    18061 agcccgtata ccatctggcg aaaatggctt tgggtatgat cctttattta tcccagaagg
    18121 taagaaacaa acagcagcag aattatcgag cgaagaaaaa aacaagatca gtcatcgggc
    18181 tcaagcaatg aaaaaattga gtgccgagtg gaaacaatgg ctggaaggag agagatagat
    18241 gcggtattta gtagtaagtg ataatcatgg agacagagat atcctcgttg acttagtaga
    18301 tcgttatcgt tctgaagtgg atatcttttt ccattgtggt gattcggaat tagaagcttc
    18361 tgacccatta tgggatacat tccaagtggt tcaaggtaat tgtgattatg gaccaggatt
    18421 cgaacagaaa aaagtgatcc aaactggtca ggatacagta ttcatgactc acggtcattt
    18481 atcgaatgtc cgatttggtt tgacacagtt agcaatcgaa gctcgagctg caactgccga
    18541 tatggtgttt tttggtcata cccatcaaat aggctgtgag atggatcaga aaatcctgtt
    18601 tttgaatcct ggtagcattt ctcagcctag aggcccgatc caaatcccat catatgctct
    18661 tatcgaaagt ttagatgacc aaatttctgt tcaatactat aaccgtgccc atcaggcagt
    18721 tgaagagttg gccttttctt tttcgagaga gcgctgaata aaaaaactct aatttttaat
    18781 aaaaataagc tttattttat gaattttgca tccggtttcg gtaaaatagt agtgttgaat
    18841 gaaatagaaa tggaggccat cagatgattg gacctatagt aagagaaatg ctgctggaaa
    18901 atcaggaaac gtttttgatt cctgcagaaa atgtggcaaa tgttatgtat catcatccat
    18961 tgtcccatgg tttacttgtg ttatcgaagg tcggttattc caaaattcct gtattaggaa
    19021 aagatgatca ctttgtagga ctagtaagtt tatcagatgt ggtcaataaa atgattgact
    19081 tgcaaacaat cagcatggac ccgttagaag gactaacggt tgctgatgtg acagaaacag
    19141 atgttccgac aattgatgaa aattgggaac tagaagaagt actccatttg ttagtcgatt
    19201 cgcctttttt accggttgtc acagaagatc ggattttcaa aggaatcatt acaagaaaag
    19261 agctgcttaa agccgtaaac ttcatggtgc atgaattaga acgtcgtaat atattgtctc
    19321 cgaaaatcga agaattaaaa gaacaaatcg atatcgtgag ctaaagaacg agaaactaaa
    19381 agataatcaa aaaataagta gaaaaaacag cgtaccagtt ttgactttat cttgaaaaag
    19441 aggctgggac aaaagc
//
LOCUS       DACDYI010000063        18840 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.063, whole genome shotgun sequence.
ACCESSION   DACDYI010000063 DACDYI010000000
VERSION     DACDYI010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.063"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(21..251)
                     /locus_tag="IWM52_002131"
     CDS             complement(21..251)
                     /locus_tag="IWM52_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974362.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(687..1253)
                     /locus_tag="IWM52_002132"
     CDS             complement(687..1253)
                     /locus_tag="IWM52_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9974363.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1253..2209)
                     /locus_tag="IWM52_002133"
     CDS             complement(1253..2209)
                     /locus_tag="IWM52_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAP9974364.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2209..2865)
                     /locus_tag="IWM52_002134"
     CDS             complement(2209..2865)
                     /locus_tag="IWM52_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP9974365.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3235..5253
                     /locus_tag="IWM52_002135"
     CDS             3235..5253
                     /locus_tag="IWM52_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9974366.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            5680..8094
                     /locus_tag="IWM52_002136"
     CDS             5680..8094
                     /locus_tag="IWM52_002136"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAP9974367.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8442..9995
                     /locus_tag="IWM52_002137"
     CDS             8442..9995
                     /locus_tag="IWM52_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP9974368.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10376..12160)
                     /locus_tag="IWM52_002138"
     CDS             complement(10376..12160)
                     /locus_tag="IWM52_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9974369.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12175..13896)
                     /locus_tag="IWM52_002139"
     CDS             complement(12175..13896)
                     /locus_tag="IWM52_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9974370.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13996..14757
                     /locus_tag="IWM52_002140"
     CDS             13996..14757
                     /locus_tag="IWM52_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9974371.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            15724..15992
                     /locus_tag="IWM52_002141"
                     /pseudo
     CDS             15724..15992
                     /locus_tag="IWM52_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            16664..>18840
                     /locus_tag="IWM52_002142"
     CDS             16664..>18840
                     /locus_tag="IWM52_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974372.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVL"
BASE COUNT         6186 a         3343 c         3771 g         5540 t
ORIGIN      
        1 ctcattaaaa agcataacca ttactccgat ctgtttttaa aattagtttc tacctctaat
       61 atatatcgag tttttatttt gccttcagag aatactgttt ctttttttgc agttacaggt
      121 tgtttatttt cggaaaaagc taatatagct aagattgaaa catccatctg gaatttatct
      181 tttttactgc tttgatcata aaagtctgca tattcatcac tgatattttt tctaataaat
      241 tcttccatca taaaaatcac ctcaaaacga ttatacgaac ttacgttctt gttgtaaagc
      301 gatatttgag gggcaaaaaa ggggcataat ttgactgata ttaagttagc tattgatata
      361 tgaatgacat acaaaaatat aaaatctttt aaaaatgctt atatatcaat gtttttgcca
      421 ttcttttata tatctgttat atgtcaaaaa aatgtgttca atttcaattt gtatttgaat
      481 aaagaggttg tgaaaaagct gtcctgcatc aagtaataag aacagccaat caaaaacagc
      541 ttctcatgtt tttgatttgg ttggacttat taccgcagat ataagctttt gagcaccgtg
      601 aataaagagg ttatgaaatc aacgctctaa cctgagtatt agagaaaaaa acgcaaaaca
      661 tatcatttat gttttgcgtt ttttatttat ctttttttct caatacaata taaaataggc
      721 ggctggttct tttggttgat aaaccgataa ttcagcacat tatatgtttc ctgtggcagt
      781 ttggaacaaa aagaatcaac catatccagt tctttttctc caccttcatg accatagtag
      841 acgacaagga tcaatcgtcc gcgaggcaca agtcgtttca atatttcttc catagctgtg
      901 cgtgtagtct ctggcatagt gatgattgct ttatcacttt ttgggagata ccctagattg
      961 aaaatccctg ctttgagatt ttgttgttca gctatcactt ctcctaacgt ttcgtggcct
     1021 tgtagaaaca atgagacacg ttcttctaac tgacgctgtt ctagcctttc acgagtagca
     1081 tcgattgctt gttgctggat atcaaaacta tagacatgac cagtttttcc gatgtgttct
     1141 gctagaaaaa ccgtatcgtg gccattgccc atcgttgcat ctacgacgtg atcccctggt
     1201 tgcaagattt cttgcagtaa tgtatggctg aaatgcaaag cagtttgcaa catcagacgc
     1261 tcaccttttt ccgtacatca tattttcctt ggaagctatc tctgcgaaga agttcttcat
     1321 caattgcatt caagacttcc cattttttca agctccacat tggtccaatc aaggaatccc
     1381 acggcgcatc ccctgtcaaa cgatggatga tgatctcttt tggtatcatt tccagctggt
     1441 cgcaaatgac attgacatag tcaggacggc tcattaattg aaggcgacct tcatgatagt
     1501 cccgcaacat tctcgttttg cgcatcagat gcatcaaatg gagcttgatt ccttggatat
     1561 ctgaatctaa aatggtgcgt ctgacatttt ctttcatcat ttctaaactt tcgcctggta
     1621 atccgttgat caaatgcgta caaacacgga tattatgttt gcgtaattta gccacgccat
     1681 ctaaataagt ttgataatca tgtgctcggt tgatcagttt tgaagtttct tcaaaagttg
     1741 tttgcaatcc aagttctacc cataaataca tccgctcgtt cagttcagca aggtaatcaa
     1801 ctacttcatc tggcagacaa tccggtcgtg taccgactga taatcctacg acacctggta
     1861 aattgactac ttgctcaaat cgttctttga ttatttcaac tggcgcatgg gtgtttgtaa
     1921 aattttggaa ataaacgatg tattgttgga catgaggcca tttttgatgc atcatgtcga
     1981 tttctttctg gaattggatt ggaagagggt cttcaggtgc aacgatcatg tctcccgaac
     2041 cagatacact gcaaaaagta caaccgccat gggcaacagt cccatctcga ttcggacagt
     2101 caaatcctcc atcgatcggc accttgaaaa ttttcccgcc aaactcattt cgtaaagcgt
     2161 aattccaaga atgatatctt ttatttcctt ctgaataagg aaaagtcatt aaaagtcctt
     2221 ctttctgtaa gagttatcta tttgttttct tttttctcta tacactggat aagtcaggca
     2281 cagccaagaa agactgagta agtagccgcc gatgatatca gtaggaaaat gaacgcccag
     2341 ataaatgcgg cttatcccga cacttactat aataaacatc atcagcaatt gaagcaatgt
     2401 gcgtaaggtt cttttttgaa caaaagctgg aagcagaaat agtaaagaac cgtaaagaat
     2461 catactgcct gctgcatgtc cactagggaa actgtaactt gtttcaacta ctaagtgcgc
     2521 caatgaagga cgctctcgca tgaaaaatag tttgatcacc gggttgatga ttccagcaat
     2581 tacaataaat ccagaaacga accaaaatgt ttctgccctt tttttcttat accacatcca
     2641 taaagccgca caagcaaata aaatgatgac ggtaagagga ttaccgaatt tagtcataaa
     2701 gagttggtag ctgttcagat caggataaga agctctgaca atgcctgtaa accactgatc
     2761 gaaaccagaa agccacgaca gatggaactt tacgatccag cccaaaatga aaaacaggac
     2821 aagaaagaag ctaccagcca cctgaaatga tgttttatat ttcatacaat tcctcctttg
     2881 aagcaatgac ttattatagc atttttggtc cagcgaaaaa aacggtaaag agataaaacc
     2941 aatgtatgat tcctctttga ataaccaata ttcttataaa aaaaataact gtaatcggaa
     3001 agacattttt tggtaatgca aatgaaatgt tagggaaata aaatcagtgt ttttcgaaat
     3061 ccttttaaac aagaacgtag acgtaggaag aacttgaaaa tgaacagaag ttgttcatta
     3121 cggtaaatta caatatgatt acaaaaaaca acaaagctct taaataactt ttaataaata
     3181 ttaagagaag ttataattat taataataga taaattgaag ttggaggata gaagatggaa
     3241 aacattgcaa gaaaagaacg gagacgttta gaacagacca aacgttttcg aaaagcaaaa
     3301 cgaggggcag caatcgtggg aactgcgatg gtaggctgtt ctgtcgctgc cccattagta
     3361 cagccggttc ctgttcaagc ggatgaaaca cctgttcagt tcggcgcaag aatcaataca
     3421 gcggcattta tcacagaaat tgctacttat gctcaaccaa tcgctcaagc caacgatcta
     3481 tatgcatcag tgatgatcgc acaagcagtt gtagaaagcg gttggggcag tagtgcgtta
     3541 tctcaagcac cttattataa tctttttggg ataaaaggca gctatcaagg acaaaccgtc
     3601 tacatggata cgcttgaatt tttaaataat caatgggtaa ataaaaagga gcctttccga
     3661 caatatcctt cttttgctga atctttcaat gacaatgcct atgtacttag aaatacttca
     3721 tttggcaacg gttattacta tgccggtact tggaagagca acacgaaatc ctatacagat
     3781 gcgacagctt gtttgacagg gcgttatgcg actgatcctg gttacgcagg caaattaaat
     3841 aatatcatcg caacttacgg attgacccaa tatgatacac cagctagcgg aaacgcaggc
     3901 ggtggagcga ctacaggtgg caataatact ggaagtgcag gtagtactgg cggttcaagc
     3961 aattctggta atacaaactc aggttcagca acttcgacca cttatactgt ccaatctggt
     4021 gattctgttt ggggaatctc aaataaattc ggtatcacaa tggatcaatt gatccaatgg
     4081 aacaacatcc aaaacaattt catttatcca ggacaaaaac tgacaatcaa aggtagccag
     4141 gaaaatggct cttcgacgaa taattccggt aacaatacca actcttcagg aaatgctggt
     4201 acatcaaacg gaggacaaac aactggtgcg aaatacactg ttcaatctgg tgattctgtt
     4261 tggaagatct caaatgatca cggaattacg atggctcaat tgattgaatg gaacaatatc
     4321 aagaacaatt tcgtttatcc aggacaacag ctaatcgtat caaatggcgg ttcttctaat
     4381 gctggagcaa atactggctc aaatactggg aatacaagtg gctctagttc gtccagctca
     4441 agttcttcta atacagcttc aggaacgaaa tatacagtta aagccggaga ctctgtttgg
     4501 agcgtctcaa ataaatacgg aattacgatg gctcagctaa tccaatggaa caatatccaa
     4561 aataacttta tctacccagg acaacaactg actgtttcaa acagtggatc aacaaacagc
     4621 acgtcaggta atacaggttc gtcaaccaat acaaataaca gcagttcagt taatactggc
     4681 agcagttcgt ctcaaggcac ttataccgtg aaagcaggtg aatctgtttg gagcgtggca
     4741 aataaaaacg gcatcacgat ggatcagtta atcgaatgga ataatatcaa aaacaacttc
     4801 atttatccag gacagcaatt gatcgttaaa ggtggaactt cggcaaatac aaatacaggt
     4861 tcaactactt ctgcagcgaa accaaataca ccgaatacgt ctgctacgac aagtacatca
     4921 tccactggta atacgatgta taccgtgaaa gcgggcgagt cagtttggag cgtagcgaac
     4981 aaacatcata tcacgatgga tcagttaatc gaatggaata atatcaaaaa taatttcatc
     5041 tatccaggac agcaagtgat tgtgaagaaa ggttcatctc aatcaacaaa ccaacaatca
     5101 gttactggtt cgaaaaccta tactgtgaaa tctggagaat ctgtttggag tgttgctgac
     5161 agtcatggaa ttacaatggc tcaactaatc gaatggaaca atattaagaa caactttatt
     5221 tatccaggtc aaacgttgat tgtagctaaa taaaatagct gttcgaagaa ataacgattg
     5281 aattccacaa aaatatttgt tactataagg ctacttaaat atgacaacac ttgaaaagga
     5341 agtagtaaaa agactgaagt ttcagagagc gcatggctgg tgagaatgtg cacggatgct
     5401 tttgaactcg cctttgagtt gcccttgctg aatacccgag taagcaagcg gcggtgacgg
     5461 ccgttacaac gttcgaggcc ttttcactaa ggtaaaatta ggtggtaccg cgtagattac
     5521 gtcctataaa aagagcaatc tttttatagg atttttttat ttcttttgtg aagtttgaga
     5581 aaacaaagca aaaggaaata aaaataaaca gattctgccg gttttttcgt tatatttata
     5641 atagaaacaa gtgttttcaa tagaacagga ggacagcaaa tgagttacaa tcataaagaa
     5701 attgagaaaa aatggcaaaa atactgggca aaaacgaata ctttcaacac ccatgatgat
     5761 cctgaaaagc caaaatttta cgcactggat atgtttccct atccatccgg tcaagggcta
     5821 catgtagggc atcctgaagg ttatacggca acggatatcc tttcacgtgt gaaaagaagt
     5881 caaggataca acgtgctgca tccaatgggg tgggatgcat tcggtctgcc tgctgaacaa
     5941 tatgcgctag atacaggaaa cgatccagct gagtttaccc aaaaaaatat cgaaacattc
     6001 cgacgccaaa tcaattcttt aggatttagt tatgactgga atcgcgaagt gaatacaact
     6061 gatccggagt attataaatg gacacaatgg atctttacga agctttatga aaaaggtcta
     6121 gcttatgaag cagaagtagc ggtaaactgg gtacctgaat taggaacagt tatttcaaat
     6181 gaagaagtga tcgatgggaa aagtgaacgt ggtggctatg atgtagtcag aaaaccaatg
     6241 cgccaatgga tgctgaaaat cactgcttat gctgatcgct tattggatga tctagaatta
     6301 gttgattggc cagaaagcat taaagaaatg caaagaaatt ggatcggtcg ttcagtagga
     6361 gcaaatattg aatttaaagt ggctggtacg gataaatcct ataccgtatt tacgacacga
     6421 ccagatactc tattcggtgc aacttactcg gttcttgcac ctgaactaga tcttgttaga
     6481 gaaattacga cccctgaaca aaaagctgct gtagaagctt atatcgaaga gacagctaaa
     6541 aaatcagatc taaagagaac ggatcttgca aaagaaaaaa caggagtatt tactggtgct
     6601 tatgcaatca atccagttaa tggcaaggaa atcccaatct ggatcgccga ttatgtatta
     6661 tcttcatacg gcacaggagc catcatggct gttccagcac atgatgaacg tgactttgaa
     6721 ttcgctcaaa ctttcggact agaaattctt ccagttatcg aaggtggaga tgttgaaaaa
     6781 gctgcttaca cagaagatgg tacgcacatc aactctgaat ttctagacgg catgaacaaa
     6841 caagaagcaa tcgataaaat gaatgaatgg ctagaagaaa atggcgtagg gaaaaaagaa
     6901 gtcagctatc gcttacgtga ctggctattc tcccgtcaac gttactgggg tgaaccaatc
     6961 ccaatcatcc attgggagga cggaacagtc acagctgtcc cagaagaaga gctaccatta
     7021 cggttgccta agacaaaaaa catcaagcca agcggtactg gagaatctcc attagcaaat
     7081 atcgaggagt gggtcaatgt cgttgatcct gtaacaggta aaaaaggccg tcgcgaaacg
     7141 aatacaatgc cacaatgggc aggaagctca tggtattacc tgcgttatat cgaccctcat
     7201 aacaaaaaag aactagcaga ttatgaaaag ttgaagcgtt ggctaccagt tgacatctac
     7261 attggtggag cagaacatgc ggtacttcat ttactatatg cacgattctg gcataaattc
     7321 ttatacgata tcggtgttgt tcctacaaaa gaaccattcc aaaaactata caatcaagga
     7381 atgatcttag gcgaaaacaa cgaaaaaatg tcaaaatctc gtggtaatgt agtcaatcct
     7441 gatgatgtgg tcgaaactta cggtgcagat acactgcgca tgtacgaaat gttcatgggg
     7501 ccattagatg cttcgattgc ttggagcgaa aatggcttag aaggaagccg taaattcttg
     7561 gatcgtgtat ggcgtttgat cgttgatgag aacaataaaa tgcgtgaccg tatcactaca
     7621 ttgaatgatg gcaagcttga taaagtgtat catcaaacag tgaaaaaagt gacagaagac
     7681 tatgaaaatc ttcatttcaa tacagcaatc tcccaattga tggtctttat caatgaagcc
     7741 tataaagtgg acgctctgcc ttatgaatat atcgaaggat ttgttcaact actcgcaccg
     7801 attgctcctc acatgggtga agaactatgg gcaatccttg gaaatgatgg tggtatttct
     7861 tacgcaccat ggccaacata cgatgaagca gcacttgttg aagacgaagt agaagttgtt
     7921 ttccaagtaa acggtaaagt tcgtgcgaaa agcaatgttc cccgagactt aggaaaagat
     7981 gagctagaaa aagcagcatt agccaatgaa atagtgcaag aatatattga aggcaaaaca
     8041 gtcagaaaag ttattgcggt accaaacaag ttagtcaata tcgttgctaa ttaattggct
     8101 ctttgtcaat aaggactgat gagttgtgca aaatcaaatc tgggccagaa tgaacttcat
     8161 tctgtctcag attttttgtt atttcaaatt gattaattga ttgatcaaaa aggttttgat
     8221 tttcatgttc tcaaacacgt tcaatgtctt tttcttctga atggtcttgt aagagatctc
     8281 tgtaatcatc aaatttttgt ctattaatct tagtattttt tgatgtataa actacctgaa
     8341 attgagagta cataccaaat tcataaattt tttattagtg ggactagtga ttgtatggat
     8401 tttgacgcat aatgaatgaa gtatgagaaa aggagagaca aatggatcaa gaactattta
     8461 ttatcgaagt attatcagct aaacaaaaaa ttatcgagaa agcgaaaaaa agagcgaaaa
     8521 gaataatgtt caagctttta agcttagtat ttttctctgg tttgcttttt ttcggagctg
     8581 tatcgttcct atatcgccat atgagtgcag tagtccgttc agctgaaggg atgcaaaaat
     8641 taaataattt gtatcacttt tcgtggggag ttggcggaat aacaattgtt gtgcttattg
     8701 tttttcttct acttatttgg ctccgttacc atcaattgtc gcgtcatttc gatcaatggc
     8761 ttagtcaaag agcacaacag tttttacata ataaaccaac ttctcaagat gatgactatc
     8821 tttattttga gaagacttcg acgagtcaag cttttcggat acaaaaaagt gattgccagt
     8881 tattgctgag atcacttgat gatttaccta tctacttagg cgaaagcaaa tcaaaattcg
     8941 gaatgaacac attccatatt tttttactag aaaacgaacc cagatgggaa aaaagccagt
     9001 cttcgcctgg tggatacctg acaactaaga tgagtacatt agtcactggt tttttcctca
     9061 ttcttttagc tggaacaatc tattatggga gatacactca gccaaaaatg tcttctcata
     9121 taaatgctgc ttctcacatg acaatgaact caactaacga tacgcatata caaaagacaa
     9181 atgaagggtt gttagctgat cctaagcaaa aggtaaattt gacaacacaa aagataaatg
     9241 atctcaaact tgatacagag acgcactctc tctatatgac aacagatagt ggtgctaatt
     9301 gggtatttgt gccgattgat tcgcaatggt tgcgttttgg cgaatacacc ctgacaacgg
     9361 ggctgattcc tgaaggttat tggatggaca acacatatga tatttccgcc gatttctcat
     9421 ggtttatcta ttcttctggg gatgatgtac gtctgctgac ttcaagagat aatggtaaaa
     9481 catggcaaac gtctattatc acccaatcag ttcagccgat acgttatcga aaagcggtat
     9541 tttatggcaa cgatggtcta tttatctgta catcagccag tgggatgtct gcagaaactt
     9601 tgtacattta cactactatg gatggcggaa tcacttggat aaaaagtgga agtacaatga
     9661 tcaatcagcc agtacaaaat gtcagcttcg ttacaacttc tttaggtttt gtttcgacac
     9721 gtgaaagttt gttttataca aacaacggag gacgctcatt caaagaatca gttctgtcga
     9781 ttccggaaaa ctatcagaca ggggggatag atctttttca atcaccaaat gaagttactc
     9841 aaataagcgc gaatacgtta gagacaaaat tttatttgtt gaaaaaagga ccaatcgatg
     9901 cagggaaaat gtttgcctgc ctcttccgtt caactgataa cggggaaaca tggcaattcg
     9961 ttcagcaact atcgcaagta gaacaaggag attaaagaag agagattgtg agcctgacgt
    10021 tcagcaccga gtcacaagaa acaaattcaa aaaacagctt ctcatatttg ttgaatagtg
    10081 taactcgtga ttcggagttg acaagagcaa caagaagcga ttttgaagta tagcttctcg
    10141 tattttacaa gatcgtgtct tattgccgat aggttgtctc ttgaacaccg tttattcgta
    10201 aaaagggatt gtgacaagtc ttttgtcact acttaccgaa cgaaactgaa ggagctatcc
    10261 aaaaagtatc attagtatta ttctttgtgt ttctagtggc accactagta catttaaaaa
    10321 agctatcgat catggactaa tcatacatga tcgatagctt tttagataat gtttgttacg
    10381 ctatctgaaa ttgactatta taaagactgg cataaagcgt tggtgattgg agtagttcag
    10441 aatgtgttcc tttttcgacg atatcgccat tcttcattac aaggatcatg tctgcatttt
    10501 caatcgttga caaacggtgt gcaatcacaa aacttgtcct atcttttgtc acagcttcca
    10561 ttgcttgctg aatcaatgct tctgttcgtg tatctacgct agaagttgct tcatctaaaa
    10621 taacgacagg aggattcgct agaatgactc gagcaattgt caataattgt tgctgtcctt
    10681 gagataacgt gccgttttcg ctagaaatga tcgtatcata tccgtctggc agtgttcgaa
    10741 taaagtgatc acattgtgcg atttcagccg cttcgataat ttgttctctt gtggcatcca
    10801 ttttcccgta ggcgatatta tcagctaccg ttccttcaaa cagccaggta ttttgtaaaa
    10861 ccatcccaaa agaacgacgt agttctccac gtgaaagctt ggtaatatca agaccgtcga
    10921 attcgatctt tcctttattc agttcataaa aacgcatcag cagattgact aaagtcgttt
    10981 ttcctgcacc agttggacca acgatcgcta ccgtttgttt tggctttact gaaaaatcga
    11041 cttgattcat caatatttta tctgatgtgt aaccaaactg tacatttttg aaagctattt
    11101 ttcctttcgg ttgttctaaa agtcttgcat ctggaaggtc tggaatttca tctgtttcat
    11161 ctaatatctc aaatatccta tcgattgctg ccattgcgga ttggatcgag ttgatcacat
    11221 aagaagcggt agaaattggt tcagaaatct gattgatgta ctgaagataa gcttgtaaga
    11281 agccgatcgt cacaccacct gatagaacaa gcatagcacc gaataccgca cttatgatga
    11341 aggcgagttg attaatgaaa cgaatggccg gatagatagc acagttcata aattgagcac
    11401 gtttaaaagc gttgtattgc tttttattta cttcgtgtat agcagcttct gcatgagctt
    11461 gctgattgaa tgttttgacc actagattac cagctaagat ttcttccatt cgattattta
    11521 aaacacccag ttcatactga ttctgttcag aaatctgttt gtttttgttc gcaatcttag
    11581 ttgtaacaat tgaactgcca acgatcagca ataagacgag caaagtaagc ttgctttcga
    11641 tataaaacag catcagacca gcagcagcta ttgttacgac agaagtaaaa aattgattga
    11701 tacctgtcaa taacacttga gaaagttgat tgagaccggt agttgtccga ctaatgatgt
    11761 cacctacttg atgattgtca aaaaacgaca tcggtaatgt tttgaatttt tttgtgattt
    11821 cttttcgcac cctcaatgtg atcttttcgc ttaacgctgc cattgtccgt tcctgaataa
    11881 atgaagtgat gctgcttaag actgaacagc cgatcagcaa taagacaggg aacaacaatg
    11941 cttgtttgat atcactgaaa gaaagctgtc caatacctgt ttttcttatt tctgataaga
    12001 gatcatcgat cccgattccc atgatcaagg gcatggctac gactaatagg ttcccgaata
    12061 aactgcatag aaacaatccg taaaaatatt tatgttcagg tttgatcatt ttccaaaaac
    12121 gtatcaaagc acgcgtgttc aacgtttttt tcttttccat cgtatccctt ctttctatat
    12181 tccttgtgat tttgcaaact cctgataagt ttggcaatta gacagtagtt cttcatgagt
    12241 accttgtcca acgatttttc cttcatctaa aacaatgatg ttatctgctt gttggattgt
    12301 actgaggcgt tgagcaacaa tcacgagagt tttctccgac atgtgtttat gaagggctgc
    12361 tcttaatgct gcatctgttt tatagtctaa agcggagaaa ctatcatcga atacatagat
    12421 ctctgctggt ttgatcaacg ctcgagcaat acatatccgc tgtttctggc cccctgaata
    12481 attagaaccg ccttgagcaa caaagctgtc caatccgtca ggaagggtgt ccactagttc
    12541 ttctaattga gcgatcttga tcgctttaga caactcttct gttgaggcat attcatttcc
    12601 catctgcaag ttgcttaaaa ttgtcccgct gaataagaaa gccttctgag ggacataact
    12661 gatgtgggag cggatctgtt cttgagtcat ttgagtaaga ggaataccac caagggttac
    12721 ttttcctatc gtagggtcac taagtctcaa gagaagtttc gccaaactac tttttccaga
    12781 tccggtcccg ccaacgatcg ctgttgtttt tcctttaggt attgtgaaat caatatcttc
    12841 tagaactgga tcagagattt cagtatattt aaatgtgaca tgttcaaaag cagctgcatt
    12901 tgtctgatca aaggttggtt cagctactgt tttgatcgtc tcttcctcaa tttcgatctc
    12961 tgcttgcagt atttcttcta tccgtcgtaa tgaggcaaac gatttaggga taatgaccaa
    13021 taccatagct gcgatcatca agtaagcaag tgtcagcata gagtattcga ctacagcagt
    13081 cacgacaccg atttgtaatg ctccttctgc tacaagattt ccgccaaacc acaaaataga
    13141 agcataaacg attcccatga gcaaaaaagc aattggagtg acccaagcaa atattcggtt
    13201 cactttgatc atttgattag catagtcagc aaagctctta ttcgttcgct tttcttcata
    13261 tgattgatta tcaaaggcac ggatcatatt aatcccagta aagaattctc tcaatgtgat
    13321 ggtgatccga tccatttttg gctggatact caaagataaa ggtgcccctc gtttcatcag
    13381 tcgataaaca atccatacat aaaaaacaac agagatcaat gtaacccatg ttagtaatag
    13441 agaataacga aacatcatga gaatggtgaa aacgcaaata accggtgctg gtaagatcat
    13501 ttggcaaaat aaaacaatca tttgctggac attatcgaca tcattcgtca ttcttgtcaa
    13561 aagagatcca gaactaaaac gactgatatc cttcattgac agctgttgga atttacggaa
    13621 atatttctcc cgcgtttgga atccaaacct agcggaagcc aatgctgaaa agtaactaga
    13681 ggcaactgca gcgattgttc ctagaaacgc tacaagcagc atttcaactc caattttttt
    13741 tataactgag gcttcgccac ttgctatacc tgtgtcgatc agctttgcta ctaattttgg
    13801 tacacctaat gtaccggcga tttctaaaca gatcatcaca aaggtaagga gagcgagtcc
    13861 tttgtttttc tgtaagaatg tttttaagat agacatatta ttcctccttt tttcctgata
    13921 cttttcgatt ctataccttc aagtaacttg aaggtcaata gaaaaaagaa taaactgaaa
    13981 aagaggtgag aaaaaatgaa aaaaatgtat acgattggcc aagtatcgga actatttgat
    14041 ttgccaaaat caacgatccg ctattgggat gaacaaggat taatccgttc aagtagacaa
    14101 gaagagaatg attatcgatt gtttgatatc gatgatattt ttatgcttta tgatatcgtt
    14161 ttttatagaa aattagatat accgatcaaa cagatgaaga atttatatgg aaaaacattg
    14221 acggagttat atgagacact tgatgaaaca gaaagaagaa tccataaaga actggtggtg
    14281 atgaaacaaa aacaaaaaga gataagggag caaaaaaaac agttgaaatt gatgatcgat
    14341 acaaacgagg aagaatttcc tgtagaagag atcccttttg actgtatgat ctcaacggag
    14401 tttgaagata ttgtggaaat caaaaaattt ctacctaact attcaagctt tgggatgatg
    14461 agtatgccag cttccagttc acagacgatg tatggatttt ttatcgaccc tagtgaagta
    14521 catttattta ctcaagacgt gatttgggag aaaaaagata cggctgttta tcgtcgattc
    14581 ttattgaaat ccgagatgaa ccatgcggaa cgtaataata ttttagaaat cagagaaaga
    14641 atgtatgaaa aaggatggaa aacaggagaa gtgatcggcc agtatctatt gacgaatacg
    14701 gatgaaaaca atatcagaac tgaatattat catgcatgga tagaaatgaa aaaataggaa
    14761 atatgtgttt aagataactc gaaaatgatg gttgttttag ttataaagaa aaattttctg
    14821 gttcgattac gggtttaatg tgggggatta ttagtcgtgg atagatagaa ttttagtttt
    14881 ttagaggaaa tatgtcttta aaaatggaat ggttgcaatt cttagcgata tatacgatgt
    14941 aattgtcctc ttaaaaaagt atggaaaaat aattgtattg ggtgttatgt ataattcatg
    15001 gtgaactgag actaatgaga agaaaaattc atattgttat gagtgagatt gtatcagatg
    15061 agaatgataa attaaatagg tgcataaaag cattacactt aaaatacatt gttgcttaca
    15121 ttattacaat atgttaataa ggcaaaaata aatagatgga ttgaataaac atatttttta
    15181 atcaaatagt ccctactgtg actttcctca gctatcggtg atactaccga tagcattttt
    15241 ttgcttataa aataattata agtatttaga gatttttctt ctaacattct tcaaacaaga
    15301 aaaatcacaa aggttattgt tatttttata tattcttttt aagaatgatt gtttttttat
    15361 agaaatttta aaaaataaaa cacgttattg atggatagtt agtttgttat ttggtaatat
    15421 tatagaagtt gactggtagt gatttatttt tcgtcgtttt ataaaaatgt tattttttgg
    15481 aaaaattcac aaatatgtaa cgaatgtttt tctggaattt gctgtgttaa aaagcagtat
    15541 gactaatata aatactattg tcatttatgc aattttttat ggatagtaat tttagagggg
    15601 aatcaatgaa ggaagaatag aagggaaaaa tagttgcaaa tacgccagtt agcaaaaata
    15661 gcaatacatc ttcaagcttt aaaattcgag agaatcatct tggtagtgga atcaactaca
    15721 acaaaaggat taaaccaatt aatagataga attaccaatg gaaaaataga aaaggtagtt
    15781 attctataca aaagttgttt aattcgcttc ggttatgaac ttagtgaaac cctttgcaat
    15841 atatggtaca gaatatagaa attattgaca ttacagaaaa tgatgaagat ttaatacaga
    15901 ttgtcactat ttgtagatac cttttacaag gaaaccgagc caataaagct aagaagatga
    15961 ttaaggagtt gatggaagac gatacaagtt aagaaagtga aactacgccc aaataaagag
    16021 caaaaagagt aattggacgt ggaaaatgca agaaatgaac catcgattcg gtggacagtt
    16081 tattagtaat gacggtttga gaacatccat gacgagaatg aaggaacatc ctaaatgcgt
    16141 ttagttgaac caagtgccaa ataatatggc caaataagcg atcagttatc gatgaatccc
    16201 aagtattcta aactatatat tcattttgat gctaagcatt gatatatttt tttgggattg
    16261 atgaaggtta accaatacag ggactcactg atgaaattct agagtctgac cttggtatca
    16321 acgatttagt gattgtttct aatggaaaag tataaaagta tataagtaca ttaacaaaat
    16381 aactattagg gttacgctcc agcttgtgca aggcactggc aggaggaatc gtgaaatcat
    16441 aattatggtt aagccacacg gacaactaaa agtcatacaa agctatcgaa tcaatcaaca
    16501 gaagacaatc ggcaatcatt agctttgttt acagcttaca ttgcaacgct tgagttgaac
    16561 cgaaaatacg agggaggtaa cgcagccatt ttgagttggg gaatcttttt agaaaattat
    16621 cgggtacctt gttgtttagt caaaattata agggggagta atagtgagga acaaatttca
    16681 taaactgtta agtatgtttc tagttatgct tattgtgatc aatcaatttg ggaacataac
    16741 ggttctagct gagtctttag cagaacagcc agagcgttcg ctgacgatcg aagttgatca
    16801 agaggaggct gctttgggtg agcagttcca gttgcgagtc agaggaacac cagacctgct
    16861 aaatgcattg gtcattgata agagtgagga tttgattgaa ggggaagtga agcaactcaa
    16921 tgattacgaa cgtctgatta cttattcttc ccagcattca ggtaaatacg aattgaccgc
    16981 aagaagcggt gatattcaat caaatacggt ctcaatagaa atcagtgtac cagaagaaag
    17041 cacaagtgag tcttttaatg taccagaaga aagcacaagt gaatctttta atataccaga
    17101 agaaagctta agtgaatctt ttaatgaagc cagatcatct gagccatcta taacgcagga
    17161 atctcagatt gaaagacaag aggagctctt gaattatgac atcggcatct ctgctgagcg
    17221 ccccactttg ccgagcaggc atacacaaac gttaaaggga tacattcaag gaggcgctgg
    17281 cgatattctt gaagaatatg gggagatggt cgtacgagtt cctagagcat cgattggctt
    17341 aattgagttc attagagtgg aggtcgctga tgattcacct ttaaagccac tggcatttta
    17401 ccaagaaggc acagacgttg tttttccatt tacctttgat tatgaaggat taaatgaagc
    17461 cgctcagtcg tatacattca gtatcacgtt tgaagttgct ttctttcaat caggcggtac
    17521 tgagccaaat aatgactttc aagtgagtct tacttatgct gagcgagcag caaacacgga
    17581 attttcaatt acgcctattc cttcatcaag acccattttt tcgaaattat actatggctc
    17641 tagagaaatc gacagccaag gaaatcttgg gaatctgctg attgacgggc aagacgtgac
    17701 caacagtaat aaatttattt tgcaggtaaa ttatgaagga tcacgaaaag acggtgtagt
    17761 ggttaccgat gtactaccgc aaggaacaac tttggcaccg aatccgacta catggagttc
    17821 cactggaaat agagaaccta tttcaggtgg ggttcggatc gcagaagtaa cgaatccagg
    17881 tgaagcaaac gttagaactc attatgtttc cgaggaattt gtggacaaag tatcttttaa
    17941 taaggaaacg aatacacttg ttgtggactt tggtaatcta gcagagggtc aatcgtattt
    18001 tattgaatat gctcttgaag tgaccgatgt cgacttgttc aatgaaggaa gacaagagga
    18061 tgggttttct cggaatcgag cgagtatgac ctacaataat caggtaatga gccgagacta
    18121 ccgcttgaga atccctgaaa gtaatcttcc ggcaatctct ttaagaaaaa gtgtggacaa
    18181 aaatgttatc aatctcaatg aaaatgaact aacgtatact ctgcatgtct cgatgttttc
    18241 tggaacaaga aatacgcttg ttattgaaga tgttttggaa gaaggcatgg aatttgtgtc
    18301 atttctatca gatattccct ctttcattga tgccgaagtc aataatggaa tagttcgttt
    18361 cagtgcgaat aatacgattg gtggtccgac aagttttacc ctctctttta aggtcaatgt
    18421 agaggcgtat gcggtcggcg atcaaatttt aaactttgca acgcttgtga atagtgacaa
    18481 tcgattgaat acgagtactg taagaacaag gaaaatcgat gggcgtatta aagtaattaa
    18541 aactgatgag aatggccaaa ggctggcagg agcaaaattt gaaattttat caagcgattc
    18601 acaagtgata caagaaggta taacggcaga aaacggagaa tttttaagtg aaccgcttcc
    18661 tgtaggtagc tatcaagtcc gagaaacaga agcaccggaa gggtatcttt tagatgcgac
    18721 ggtacatgat gtttttataa atgaatactc catagaagaa gtgacgttaa ccattgaaaa
    18781 catccttgat acgggtggtg ttgaactaat taaacatgct gaaggcagtg gtgaagtcct
//
LOCUS       DACDYI010000064        18105 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.064, whole genome shotgun sequence.
ACCESSION   DACDYI010000064 DACDYI010000000
VERSION     DACDYI010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.064"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(11..1519)
                     /locus_tag="IWM52_002143"
     CDS             complement(11..1519)
                     /locus_tag="IWM52_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAP9974373.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            1678..1869
                     /locus_tag="IWM52_002144"
     CDS             1678..1869
                     /locus_tag="IWM52_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974374.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            2072..2989
                     /locus_tag="IWM52_002145"
     CDS             2072..2989
                     /locus_tag="IWM52_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAP9974375.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            2982..4055
                     /locus_tag="IWM52_002146"
     CDS             2982..4055
                     /locus_tag="IWM52_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974376.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            4203..4721
                     /locus_tag="IWM52_002147"
     CDS             4203..4721
                     /locus_tag="IWM52_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974377.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            4702..5094
                     /locus_tag="IWM52_002148"
     CDS             4702..5094
                     /locus_tag="IWM52_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAP9974378.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            5170..6105
                     /locus_tag="IWM52_002149"
     CDS             5170..6105
                     /locus_tag="IWM52_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAP9974379.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            6238..7017
                     /locus_tag="IWM52_002150"
     CDS             6238..7017
                     /locus_tag="IWM52_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAP9974380.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            7010..7327
                     /locus_tag="IWM52_002151"
     CDS             7010..7327
                     /locus_tag="IWM52_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP9974381.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            7571..9181
                     /locus_tag="IWM52_002152"
     CDS             7571..9181
                     /locus_tag="IWM52_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9974382.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            9338..10186
                     /locus_tag="IWM52_002153"
     CDS             9338..10186
                     /locus_tag="IWM52_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9974383.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            10417..10896
                     /locus_tag="IWM52_002154"
     CDS             10417..10896
                     /locus_tag="IWM52_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974384.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            10929..13157
                     /locus_tag="IWM52_002155"
     CDS             10929..13157
                     /locus_tag="IWM52_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP9974385.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            13286..14929
                     /locus_tag="IWM52_002156"
     CDS             13286..14929
                     /locus_tag="IWM52_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP9974386.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(15002..15547)
                     /locus_tag="IWM52_002157"
     CDS             complement(15002..15547)
                     /locus_tag="IWM52_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAP9974387.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(15570..16055)
                     /locus_tag="IWM52_002158"
     CDS             complement(15570..16055)
                     /locus_tag="IWM52_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9974388.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(16045..16857)
                     /locus_tag="IWM52_002159"
     CDS             complement(16045..16857)
                     /locus_tag="IWM52_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAP9974389.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(16996..>18105)
                     /locus_tag="IWM52_002160"
     CDS             complement(16996..>18105)
                     /locus_tag="IWM52_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP9974390.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT         5879 a         3298 c         3626 g         5302 t
ORIGIN      
        1 taaacttttt ctattggtaa tatagatttt cggaagggta gacaggtgcg caggtaatat
       61 cgattcctag ttttttcaat gtttgttcat catttttatt gaccatcaca gtagagtggg
      121 cttgtgctcc ctcaagttca gctagtttat cataagcaac ttgagcgatt ggattcgtta
      181 ctgcactgat tgataaagcg atcaagactt cattgatgct tagtgcatcc aaagaggaat
      241 gaagtgtctt tgatttcaat ccttgtattg tttcaagaac taatggtgac aataaaggaa
      301 tgtcatctgc gatatgggct aaatgtttta ctgcatttaa gataactgcc gctgacgcat
      361 ccatcaatga acttgtacgc ccggtaatga tgacttcatc tggtaattcg attgcaatca
      421 cagcagaagg atcttttgta ggactttgcg cttgaatctt atctgcatag tcatgggcag
      481 ggactaccac gtttcggtct tcaggacgta aaccgacttc ttccataatc agtttgattc
      541 gattaactgc ttcttcgtcg atcaatcctt ttttgaagtc gcattctgtt tggaaataac
      601 gacggatgat ttcttgttta gaggcttctt ggacaacggt atcatccacg atcccaaatc
      661 ctacacgatt cacacccatg tcagtaggag attgataaac agattcttta ccagtaattt
      721 tttcaataat acgtttgatt acaggaaatg tctcaacatc ccgattatag ttcacagcta
      781 ctttattgta ggcatcaaag tggaaggaat cgatcatatt gacatctttt agatcgactg
      841 tggctgcttc ataagcaatg ttcagcggat gtttcaacgg aacattccat actgggaaag
      901 tttcgaactt ggagtaacca gctgcatgtc ctttttggct ttcatgatac agttggttca
      961 aggaagtagc gagctttcca cttcctgggc caggagctgt cactacaacg attggtttag
     1021 ttgtttcaat atactcattt tttccaaaac catcttctga gacgattttt tctacgttga
     1081 ctggatagtc ttcgatttct gagtgtttat agaccttgat gtttcgtctt tctagtttat
     1141 tgataaagac tttcgtggca ggctgtccac tatagcgagt gataacgaca ctgttgatta
     1201 ataagccata accacgtaat tcatcgatta accgcaagat atccatatca taagtaatcc
     1261 cataatcacc acggatctta ttacgttcaa tatctccagc atagacgcaa atcagtattt
     1321 cagcttgatc tttcaatttt tgcagcaatt tgattttcgc atcttcttca aatcctggaa
     1381 gtacacgttt cgcatgcttg tcgtcaacta gcttcccacc aaattctaaa tataatttgt
     1441 catagtggtt cactcgttcc aaaatatacg ctgactgctc ttcaatatat ttttctgaat
     1501 caaaaccaat ttttttcact aggttccctc cttaagtact aaagcatttt ttcatttttt
     1561 tgaggattcg tcaactggaa gagcgaaatt cctcaaaaaa acaaagaaaa tggtttaaac
     1621 caacttgtct agatagttat tcattggtat gatagttttt gataaggaag gtgctacatg
     1681 aaaaaaagac agaaaaagaa aaacgcatat aaacactata tccgttcgat ttttaccggt
     1741 tatgaaagaa tgcttgaaga tcccgaattg gaacaattga cgtttactta tctaaacgaa
     1801 gaaacgcagc taactcgtga tgagcatcag cgcattcatt tcacaacaag agatctgcct
     1861 agtaaataaa aaataagata aaggtcatcg gattcgattc tgtcctatca gatggaaaga
     1921 ctagttgatc gaaaaaaagg ctgtaccaaa ttttggtgca gcctgtttta tggagcattg
     1981 cccgctcttt tttataacaa ttttttacag aaaatggtac tattgaagta ttaataagtg
     2041 aaaaacagat gagtaagagg aggcaagtag tatgaaagag ccagatacaa atgcaaatta
     2101 tgagccagac gaactagagg agacgaatgc ggatcaggat acaggacaag aatcgtcttc
     2161 tttttacgat atcaaagaaa aagcaagcat gaatctttcc caattattag aagacttaca
     2221 ggcttttgaa aaagctgtcc gcgaagaaga tatccctgag atctaccgtt tatacaatgg
     2281 ccgattgaat gaagaattaa aaagaagctc aaatgaaaat cacgagatcg atcaattgct
     2341 gatgcgtaag atccatgatc gttttttaca ggaatttcct tttatggaac aagtagaaaa
     2401 catctcgcca acgatgagct attacaaaat cggcacttat taccatgatc ggcccacggt
     2461 cggtattgat gcaagtctgc cagaaatttt tgttttgcct aagatcgatg aagagtggga
     2521 gaaatattcc caaccggagt caacggacta tgacaaaaaa atcaatgagc tagacgcaaa
     2581 ggtgatcact gctcagacag aaattgaacg tttagatgaa caaataaaag aaatcaatcg
     2641 acaaatgact gatttgaacg acaataaagg attcttgaat cgtaaaaaaa tcgaggaaga
     2701 aattcaagaa ttagagaaga agaagcaagt actatctaat gaaaaattag gctggcttcc
     2761 ttatattgag acgcctgaaa caatccagca gcaaaaagaa gcattgaagc aagaagcaag
     2821 agcagaccag cttcgtgcag ccatcgtaga aaaagaacaa aggcaaatca gacgctattt
     2881 cggtagcaaa gagggattcg gtcaagcaat ccatgaattc ttgatgaatt atcttggcaa
     2941 tgagaatcct aataatcaat ctaatgaagg aggttccgag tatgaatgaa cacgatgaag
     3001 cagaatggac aaaaaatgaa gaacaaacag aagatcatga agaaacgaac acttcattgg
     3061 aagaacaaac aagtaatcct ctggatgaca cagaagaaat ccctatgcta gaggaaacaa
     3121 aaatacacga aaaagaaaaa gtaactccga cgaatacgaa tactgcagaa aacacccatg
     3181 cttacgaaat aactgaaaat cccgggtatt tagaaggaaa agagcaagat catcaagata
     3241 tcgtcagttt gattgaaaca gcggaagcag aattagcgga tttacgtatg agaaaagatt
     3301 tgatcgaatc agattttcca caattattag aatcatatga tcgttttata ttgaatgaag
     3361 aaccaatctc cacgatcgga aagacaagcc tgatcgaata cttcacgtat gaattactga
     3421 aaccgttgaa tgatattggt ctggaacggt caaattcagc ttatgacaca tggaaaacaa
     3481 gacatttttc gatcaattat acgttgaatg gacaaaacga attagtcttt tcctttgtat
     3541 taccgaccat cgatcaacga taccaagtgg aatcgatgag tctcctaaac gtatcaccag
     3601 agacaatgga aatcaatgtc caagatgacc gggtgctttc tttgatccga cactggtcag
     3661 tggatcgagt attctctgca gggcagatca cgatattcaa tcacaaattg aatcaattat
     3721 tagcccatgc aagaacactt ggttttaccg ttaatcagac attactagac aatacgaagc
     3781 cacttcgttt gaatcttcag agcgaatttg aattgacgaa tgaagtattg gatgatattt
     3841 ttatcgtggc aatgaaaaat ccatcctacg atatggaaaa gatcaacgaa actacttatc
     3901 aagtacgatt agataaagga cagtctttaa cgataacaac aaacgaaaaa gatcaaacag
     3961 tcttgtcgat ctcatctgga gactacccac gatctgtcat tgatttcttt atcaattacg
     4021 aattcttggt cccattaatg gtgagaaagt cctgattgga taagttggat taccagatgt
     4081 cattcagata attctgttcc aaaggacttg cggcagttat ccagaataat tgtcacaagt
     4141 cttttctgta tgtactaaat ggacgtgaat aacttgctga tgaaggaggg atttcattag
     4201 gaatgtccac aaataaaata gcacagctta gcttattgtc tgctgcttgt gtagcaggta
     4261 ggatcgcttt tcaatttatt ccgaatttcc agccaatgac tgctattttt ttatttatag
     4321 tactttactt aagcttaaaa gatgcgttaa ttgtcatgag tctttctatt gcgatcagca
     4381 gtttttattt tggtgttgga ccatgggtca tcggccaatg gatcagctat actgttgttc
     4441 tgagtttgtt ttcaatcgtc tgcttgagaa aaacagtgca aaggaatcta tggttaaagg
     4501 gagtctgttt ttttctggcg ggcatggttt acggaatagg tatgaccgtt tttgatacgc
     4561 tgttatatcg tttgcctcaa ccttggatct attaccttca aggggtgtcg tttgatttga
     4621 tgcacagtat aggcaatgtt gtgttctttc ttgtttttct gccagtcgtc aaacgttttt
     4681 acaatcattc aggaggaaaa aatgaaaaaa ataatttata gtttgcttgc aatgagtgtc
     4741 agtatactca ttttgacaag ttgcagtaca acctatacac aatcggcttc caaccaaaag
     4801 gaggaagtaa aaacatcgca aaaagcaaca atcacgttac aagaaaatgg tcaaaacttt
     4861 tcggaaaaag aagtgttctt caaaaaaggc gatgatttac tgagtatttt gaaacgaaat
     4921 tttgaagtca aagaagataa cggcttcatc acttcgcttg aagggcactc acaggatgag
     4981 aaaaatcaac tctattggat gtttactgtg gacgggaaaa gtgctactac tggtgccaaa
     5041 gagatcaaat tgaatgatgg acaatcggtc attttcaatt tgtctaaact ataagtttca
     5101 taaaaaactg ttaaagggcg aatattgcca taattcagtg agttcagttg atgaaagaag
     5161 gaaaataaca tggaaaattt taaaaaaagc ggaatgtttt ccgtaattgc tgctctaggg
     5221 gctaatattc tagttgctat cagcaaattt gtcggctatg caatcagtgg cagtgccgcc
     5281 atgctaaacg aaagcatcca cagtatcgtt gattgcagca atcagatttt gctgttattc
     5341 ggtgataaac aagccaataa aggacagagc gagttgcatc aatttggtga aggacgagca
     5401 aaatactttt tcagtacaat cgtcgcaatg atgcttttct tcggcggtgg ggcacttggg
     5461 gtgatggaag cttttgaaaa gttgacgcat ccatcccatg aagtgggtaa tccgatcatt
     5521 gttatagcaa tactgttatt tggtttggta atcgaaggaa gttcgttacg cgtggcaatg
     5581 aaagaaatca aagaattgaa tactgaaaaa ctttccacga tgcgcttttt gcgcgaaagt
     5641 cgacacagtg aaatactgat tatttttacg gaagacttgt gtgctgtaat cggtttggtc
     5701 ttggctttag caggaacaat attgacttat gcaaccggga ttgctgcatt tgatgcgatc
     5761 agcggattgc tgatcggttt cttgctgatg ggagccgcca ttttcctagc aaaagaattt
     5821 tatagtctga tcatcggtga aagcgtgaca gcaagcgatt tatcaaaaat caagttagct
     5881 tttgaacgtc ctgagatcag tcggctgatc gatataaaaa cagttcattt aagtccaacg
     5941 gagattttag ttgcagcaaa agcagatatc gttggtaaca aagagaatga agcttactcg
     6001 gtcatcaatg atattgaaaa aaatatgcgt cagtctcttc ctgacaagaa gatgtatata
     6061 tacattgaaa cagataaata tgacccaaat tattctcgta aataaaacgt ttattcatga
     6121 attgaagcaa aaatgagaaa aatataatct tgtttttaaa taaaacggct acaatttagc
     6181 gaatttatgt tacaatgagt ccgctgttta tttgttgtaa ttaaaggaga tattttaatg
     6241 aacaaaaaga aaagtgcgcc atggcttcaa gctttgcatg cagtgatgcc actttgtata
     6301 agctatatac cagtaggatt agcttgcgga gtattgttgc aaaaagttgg atttgatcct
     6361 ctcttatcag gactgttatc gattctggtc ttttccggtg gcgcgcagtt tttagttgca
     6421 tctatgctta cgacgcaagc atcatttgca acgacattat tgatggtttt ctttttagaa
     6481 ttacgctata cattattagg agcaagtctt gccggtttta tgaaaaaaga aaagcggacg
     6541 tttttggctg tcttttctca atcactgaat gatgaaaact atgcggtgaa ctacttaaag
     6601 ttttcaactg acccttcctg gaataaaaga aaggctttat atgtcaattg gttctcaatg
     6661 actgcatggg ccggtagcaa tatgatcggt aattttttcg gctcggtgat tcgagtagat
     6721 gctgaccttg tccattttgc tttgacggcc atgttcattt tcatgtttat catgcagatg
     6781 aaaagcgcac tactgatctt tacaggttta ttctcaggcg tattaggggt cgtattcatg
     6841 gtattattcc aaaatacttt cggattgatt gcagcaacag tcattgcttc atttgccgga
     6901 tttatgctgg agaaggcatt caaaaaagaa aaagctaaga aactaagaga ccgcggtaaa
     6961 gatgtcaatg agccgctctt caacttcccg gaacaggagg ccaatcacca tgactaaact
     7021 atatttgcta cttttagttg cttgtctgtt catcgtggca tatatcccgc gcttgttccc
     7081 gatgctttat tttactcatc gaaaagtgcc atcctggttc agtgactgga tgaaatatgt
     7141 accggtggca ttatttgcgg cgttagcttt taaagatgtt ttcattactc atgaacatct
     7201 ggatattgct tggaatatca aaatagctgc aatgattcta gtagcaggcg tggcttacaa
     7261 aacacgttcg atggcgttgt cagttttgac aggacttgcg tctgtctttt tgctgtctat
     7321 gctttaaact gttttatttt attaaaaaca gagattgcga cataagttgt tcaaactgat
     7381 ctatccgaac tgcatttgca acagtcaatt ctggatgcaa gtttattcgt agggataaaa
     7441 aagattagtg ttggcttatg atgcaagctc ttttttattt taggaattag agagggattt
     7501 gtccttctat gattttttgc tataataggg aagatgttta ttttatgaaa gaatagttga
     7561 gggaatttaa atgaataaaa aattgatgca aggaacattt tggctaacct ttgctaatct
     7621 tctatgtaaa gttttagggg tagtctattt gattccctgg ctaagtatga tggggaacaa
     7681 tcaagatggt atgcttgcta cgacattgta taatgttggc tatcttcctt atggattgtt
     7741 cctgatgctt ggtacagtcg gatttccaaa tgcgattgca aaaaaagtag ctgtagccac
     7801 aaaagaagga gataacgctg cttgtcggct gatttttaga agtacgatca atatcatgtt
     7861 tgtcatcggt atcgcctctg ctgcactgat gtatttattt gctcctttac tagccggaat
     7921 cagtccgatt gctaacgtta ataatgggat actagctatc agaagtttat gcccatcttt
     7981 gattgctatc ccaattctta gtgcaatgag aggatatttc caaggaaaaa atgatctgcg
     8041 gccttatgga acttctttga ttatcgaaca ggctgtgcga gtgatcgtta tcttggctgg
     8101 tacttattac ttgcgcgtat tgactgacgg aaccatatta gaagctgtat tgatcagtac
     8161 agtcgcttct ttcttcggtg ggttagctgc gattatccat atgtatatcg tcggacgcag
     8221 aaaagacttc ttcgaactta aagatttttt gatttccagc cgttatttcg aaagagaaaa
     8281 ccgatctgcc tctgtctcta tcattcggga aacactaccg tttatttttg tcggctcagt
     8341 cattactatc gttcaaatga tcgaccaagt aacgatgaaa ccactgcttc actttttccg
     8401 tccagagatc gcagatcagc aactggaatt cttattcagc cgtgcttctg tcaatccaaa
     8461 taaattgaca ttgatcctga tctccatggt aggaacagta gctataagca gcttgccaat
     8521 attaagcacg atgaagaaaa aagatcggat acaaatcgaa aaaacagtag gagatagttt
     8581 ttcgattgcg ttattgattc ttcttccttc attgactgga atgtctttat tagctggacc
     8641 actatacaca ttgttttttg gctatgatcc tgaaagtgtc ggatatttcc aaatggcttt
     8701 gctagcttct ttgtttttct ctttattcac gatcctatct acgatgcttc aatctttgaa
     8761 tcaccatcgt tttgcgatcc gattaactgt agaagcaatt atcctgaaag tcatcttcca
     8821 agtaatcgga ttaggattag ttggcggata cgggatgagc ttatcaaatg gcttagcatt
     8881 cggtattgtc tttgttcgcg gctatcactt tatgtgcaaa gaataccgaa tagcaccttt
     8941 agcaaagatc agcaattttt tcttaaaaac tttccgctcc accctgatta tgcttgcagt
     9001 atgttttgcc gttttctttt tgctgaatca gcgattgtca atggaatcaa aaagctatgc
     9061 aatgatttat tgtgtggctg taggaagtat tggcggctta gtatttttat tttctcaatt
     9121 cggaaaaaat gggtttcgaa tgttgaaaaa tgttaaacat catagcagaa gcaaagaata
     9181 ggttaactaa tcgttttttg ctgtcacttg aaaaacggca actcgtatga ttacgcttgt
     9241 cgtttttcta ttttccaatt ttagtttcat atctaatctt acttatgata tagttataag
     9301 gtctggaaaa attctttgaa acgaggaaat taaacacatg aaacttgcag tagttacaga
     9361 tagttcggca tatctgccgg aacgtatcaa gaaccatccc gacttattcg ttattccaat
     9421 ccctttgatt atggatggga aaatttataa cgaaggaatc gatatcgaag cggatgaata
     9481 ttatggactt ttaaaaaaca gtaaagattt tcctactact tcacaacctg tcgtagggga
     9541 agttttgggt ttatatgagg aattgaaaga gaaagggtat gacacgattt taagtatcca
     9601 tctttcttca gggatttctg gctttgtcaa cacattattt gcgatgaaag atgacatcag
     9661 cggagtaacg gtcattcctt atgactctaa gatcactagt atgccgatgg gacacatggt
     9721 agaagctgcc ttagacttaa atgatgaagg tcagtccctt gatgaaatac ttgcacatat
     9781 cgatcgaatc cgagataata cctacgctta tctgattgta gatgatttga acaatttagt
     9841 acgaggagga cgtttgacaa atggtgcagc gctgattggc agcttattaa aaatcaaacc
     9901 gatcctgaca ttttctgaag ggaaaattgt tttattcgaa aaaatccgtt caagtaaaaa
     9961 agcatttgct cgagctgaag aaattattgg tagaagaaat aacgaaatac aaccgccagt
    10021 caaactttac gtgatacatg caaataattt agaagtagcg attgcggaga aagagaaatt
    10081 gcagaaaaaa tatccaaatg cagaaatcga aattggtcat ttcggaccag tcattggtac
    10141 acatttaggt gaaaaagcca tcggcattgc catttcagcg caataattcc ttttattcaa
    10201 aaaacatacc aaaaatattc caacatcatc ttggactttc tacatgttac taatgagcgt
    10261 gtaaaagaac gagatggtgt ttttatttct atcaatgaag ctctttttct ttgcaatcct
    10321 cacttcaatc ctctataatg tcgcatatag tttagtaaat gagtaaacta ctaatttact
    10381 tatttactaa aaataaaagg atgaggagtt aaaataatgg aaataccaac aaatttaaaa
    10441 cataaaccag taatcgttgt agaagactat gataaagttg atggaagaaa tgcattaaat
    10501 acggatgcaa aagggctttc actgggcctt gctcagtgga atgatcgtgg aaaagtcgat
    10561 atctcaggca aggtatggcg acatactgga gaaaaatggt cacgacagtc ggaagaatta
    10621 ccaatcacac gtattttaga tttagcgatc ttagctgcac aaggaagttt atacttccaa
    10681 gatgcttatc gttatgaaaa attttatgat ccagaaaatc cagtcgtaga catcattgga
    10741 ttacaaggga accgaatgac ggtagaagta gataccaaaa atccaaagat cgatgaagac
    10801 atcattttgt attacgatac actacaaaaa gacggagaac tgattgggga acgattccgt
    10861 attttaaaac gtcttttaga tgagctagga tattaataga cagagatgat caaaagggga
    10921 agagaagcat ggagaatttc tcactaacag aaaaccgaac cttttcacgg ctagaaaagc
    10981 aaatgatctg gaagaaaccg aaaagccatc ggacaagtgc agaagaatgg cggatcgcct
    11041 ccgaaatcaa agggaattgg caagatcctg aaatgaaaat cagtaacgta cttttggaag
    11101 gagatgccgg gtcggggaag acacaattag ccaaagcact atctgctgat ctgcaacttc
    11161 cctatacaaa agtgacctgt tttgcagata tggacaaatc agatgttttc ggagccttat
    11221 tacctgtagt agattctaat aaggaacaag accaagaatt actagaagct atttaccaaa
    11281 cagatacact tgaagctgta ctgacactga tccagaacca ttacggcatt gatccttttt
    11341 ctgctaaaga aaaattagca gagctagttc aaaggatcga agctgattat ccagatgcaa
    11401 tccattacag ctattaccct tctgaaatcg ttcgagcgat tgaaaaagga tatttattag
    11461 agattcaaga gccgactgtc atccgagatg cctctgttct tgtggcactt aactcagcgc
    11521 tcgaaccaaa tgggatgttg aatttaccga caggaatcat tcgtcgccat ccagattgcg
    11581 tcattgtgat cactacaaat cgaaattacc aaggaaatcg cccattaaat gaatctttaa
    11641 gagatcggat gcaacatgcg gaaaaaatgg atcttcctgc cttagaagtc atggcagaac
    11701 gtgcgatggc gaaaactatg atccaaaaac aagaacttct cttaaagatg gcagagatta
    11761 tccgtttgtt ggatgaaact gcaaaagcta acgccatcaa aggtgtagca ggaatgcgtt
    11821 cttattttta ttgggcaaat acctataagc aaggacaaga tttactccag tcgatttatc
    11881 caaaagtttt gtataaatta tccactgatt cagatgaatt ggcaattctt gaacaagctt
    11941 tgtctgacag tggattgcta ctggaacttc aggacatatt gagaaaagaa aaatggggtc
    12001 atcctcaatc tgagcataca aaaggaagaa ccattagcgc cgaagaagca aatgaacgaa
    12061 acatcactga aaatgctgaa gaaattctgc gcttagatga aaagaaatcg ttgtctgctg
    12121 aagaaccaaa tgagggagaa gagaaacaag aagaaacaat agaaaaaaac gagattcctc
    12181 ctcaaatgca aaaagaacgc agtagcgata tcgaaggaaa aaatcaaaaa gaacaacaaa
    12241 caggagatga ttctcaagaa atgtctgctt ctgacatgga agcacatgat aaggaaatca
    12301 agaagcaact gaataaagaa gcgcgacaaa tcatgaagga aacgattcat gaaaaagaag
    12361 gtctcatcgt tcaccgacca gctttccaag gaaaaacgcc ggaagcacgt gagctacaca
    12421 tgcaagtaat gccaatagta gaatcactta gccggcaaat tttagagtta ctggataatg
    12481 aacaaacaga gacgtaccaa aaaggaaaat acgaaggtca gcggttcaat gcaagcaaag
    12541 tagcatatgg tgatttgaga aattttgaca aaaagaaccc gccacatgag caaccttctt
    12601 tagcggtagc tgtacggatc gatgaatctg ggtcaatgat acgggatgat cggatcgaag
    12661 cggcaaaaaa agcagcgatt gccattgccg aattttctaa gaaagtcgat atcccattgt
    12721 tgatttacgg agatacagca gaccgttcgt caagagaaaa gacttctttg ttttcttata
    12781 aagaatttga agacggattt caatggttaa atgaaaaatt agtcactatg aaaccaaggc
    12841 aaaacaatcg tgatggtgct gcacttcgtt tagcagcaga aaaattaaac agacaatccg
    12901 caacaaccaa actgttgctt aatattagtg acggtcagcc aaaagcgttg ccggattata
    12961 caggcaaaaa agcaaaagaa gatatccagt ctgttttgaa agaatatgaa cgtcaaggaa
    13021 tcctatttgt ctccgcagct attggccaag acaaagaaga aatcaagtcg atctacggtg
    13081 aaagtcgttt tgtagatatt actgacttga atgaatttcc caagcaaatg attcaactga
    13141 ttgcccgcta tctatgataa aataaagctc aacgaacgca cttagcagat tcgttgagct
    13201 tttcgtttct agttaccggt aacaaggata cttctaaaat agaatcggtt ataatcaaga
    13261 gggaccgtta aaggagtgaa acgatatgat acaagaacaa aaatggtgga aagaagcagt
    13321 aggctatcaa atctatcctg ctagtttcaa agatagcaat ggcgatggtc aaggagacat
    13381 caatgggatc cgtgaaaaac ttaattattt gaaagatctt ggtatcggct ttatctggat
    13441 caatccgatc tatcaatctc catttgtcga taatggttat gacgtaagtg actatcaaga
    13501 tatagaagag agattcggaa cgatggaaga ttttgattta ttgctaaaag aagcacatga
    13561 attagggatc aaagtgatta tggatcttgt catcaaccat agttcggatc agcatcaatg
    13621 gtttgaagaa tcgaaaaaat ctaaagaaag cccgtatcgc gattattaca tttgggtcga
    13681 cggggtagat ggcaaagaac cgaataattg gacatcgatt ttcggcggtt ctgcttggga
    13741 gtattctcat gaaactggac agtattatct gcatgttttt gcaaaagaac aaccagactt
    13801 aaattgggaa agcgagaaat tgaaagaaga actattcaat atgatccgat ggtggttgga
    13861 taaagggata gatggatttc gtttagatgc gatttctcat gtaaaaaaag atgaatattc
    13921 agtaaaagcc acagagaacc ctttctcacc ctttcaaaat gtctcaggaa tcgaagaaca
    13981 tttgaccgat ttgaaacatg tttttgaaga gtacgatatc atgactgtcg gagaagcaag
    14041 tggtgtcact gcagaagaag gaccacaatg ggtagggaaa gacggttatt tcgatatgat
    14101 attcgaattt gatcatatcc atatctggca gcaagaaaaa gaagggcaat tagatgtcct
    14161 taaattgaaa cttgcattaa gcgcttggca aacttcattg gatggtatcg gatggaacgc
    14221 tttatatatg gaaaaccatg atgttcctcg tgccgtttca gtgtttggag atactcgtcc
    14281 cgatttttgg gctatgtctg caaaagcaat cgctatgatg tatttctttt tgcaaggtac
    14341 gccatttatt tatcaaggac aggaaatcgg aatgacgaat atgccctttg aatcgatcga
    14401 tcaagttgat gcagtagatt cgaaaagact ttataaaagg ctgcttgcgg aaggtaagac
    14461 aagagaagaa gcattggata tcattcgtga aacaacgaga gacaacagca ggacacccat
    14521 gcaatggact tcggaacaat atgctggttt ttctacacat gaaccttggc tgatcactaa
    14581 tccaaatacc aaaacgatca atgtagaaca acaagaatat gaaccagagt cggtcctgca
    14641 attctataag aacatgatcc gcattagaca aaccaataaa ggtctgatat acggttctta
    14701 taaagaatat cttcatgaac atccacaact ttatgtctat gaaagatatt tagaagatga
    14761 acaatatctt attatggtaa acttaacaga atcacttgct gactatgaac ttccaaaaga
    14821 agcagatcag tcctggacat tactcttgtc aaattcctct agcggagaat ttgaagctaa
    14881 aggcatacta gcaccttatg aagctcggtt atataaaaca aacaagtaaa acttcctcgt
    14941 taaagttcat tagttggact ttgcgaggaa gtttttcaga ttgctctgtc aattactcat
    15001 ttcaataatt cgctctagga ccaccgatat atttaggacg agagcgaagg gctgtcacgt
    15061 gagcttgtcc aagggttttc aaacgaggtc tgacaggtac ttggacatgt ttgatatgca
    15121 taccgattga tgtgtcaccg atatcgatac cggcttgtcc aactacgtgt tccacttcga
    15181 caggatcatt gaactggtca aaagcagcaa cagaacaagc acctccagca tgtagtgcag
    15241 gaacaacaga gacaatttcg aaattttttg cttctgccac tgttcgttct actactaatg
    15301 ctcggttcaa atgttcacaa ccttggacag ctagcgcaat ttctttagga tccaacaact
    15361 cttttaacgt acggatgatc cattgaccaa cttccgcact ggaatttttt ccaatatgtc
    15421 ctccaacgac ttcgcttgtg ctgcatccta aaacaaaaag atcacctttt ttcaagtgtg
    15481 cctcagccaa tatgtcatta actatttcag tcaattggtc ttttaattct ttttctgaca
    15541 acttcatttt ctaaaacctt ctttttcaat taatattgct tccgtgcttg aatacgttga
    15601 agacttgcag ttataggaaa ggcaagtccc attccaatca gattttggaa gatgtttcct
    15661 ggtatagaag ccactccagc cggccaatca tataaaatcc aaccggcgat accatagccg
    15721 atgatcataa ctaagcttcc taataaaagc cctaatcccc acatacgagt agaagatttg
    15781 ctgaaagaac cagcaacata tccttgtaac ccgtggatca ctaatgaaaa aactgcccac
    15841 tgcggatatc ctgaaatcaa atcgatcaat agaccactgg aagcaccaac aactaatcca
    15901 cccgttggac cataaagaaa agcagcggta tagatcccgg cctcgcataa tgtcacaaat
    15961 ccattcgtag ccggtacagg gatcaaaatt gttaatgaaa gggcaactgt caacgcaatc
    16021 agcatagcat agatcgttaa ttgcttagtt ttcatttctc attcctcctt tttgaatacc
    16081 gccagaccag acacttccgg tatgatcgtt catcagcacg ccattagaaa tacaatgggc
    16141 aacaaatttt ttactttctg ctactgcttt ggtaaaatct gcccctaaca caagattcgc
    16201 tgttattgca gaagaaaagg cacaaccggc cccgtttact gttgtagtgt cgattttttt
    16261 atgagaaaat acctcataat gatcaattga atagagaaga tccacagctt catctccagc
    16321 catccttgtg ccgcctttga tcacaatgtt ttttgttcct gaagcagtga gttctttagc
    16381 gagatggatc atttctgtta acgaagaggg aagagcttga ccagaaagta aagcagcttc
    16441 tgccaaatta ggcgtaatca tcgtaacaag tggaaaaagt tcttcgatga gctgcgaaat
    16501 atacaattga ttgatcacag cttcagtttc tttgaatgcc aaaacagggt ctaaaatgat
    16561 tgggatattt tgttttgttt ttatgaagtc tcgaacaacc atgattgctt cacttgaatt
    16621 caataaccca attttgatac catctaatga agtcatttta tcaatcgaaa ctaactgtcg
    16681 tttaagcaat tccaaagata tagattccag cataaatcgc tcattaatat ctagtgcccc
    16741 tatagaagtc aaggcgctta gtccgaatag accaaaattc tcaaaagttt ttaaatctgt
    16801 ttggattcct ccaccagcaa atggatcaga accgccaatt gtcaagattc ttttcataac
    16861 attttcctcc atgaataaga gtataacgaa attttcaaaa aagaaaaaca gccaattggc
    16921 atatttttat ctcatccaat ttaaaaaaac aaaaagaaaa gaaagaccaa aatggatctt
    16981 tctttcctta aatagttagg acttttgtga ttttaattct ttctcgattt gaagccgttg
    17041 cactttcatt gtagctgttc gcgggattgc atcaaaatca cggatgatag ggacgttcaa
    17101 gcgaggaagt tcatggactt gctcccacca tcgattccaa tccatttctt tatcaggagc
    17161 taaagcaata atcggttgtg gagaatgatt tttatccctt acaatcacta cttctgccaa
    17221 aaaatctaac gaatctaata aaaagtcttc aatagctaat gtactattga tcgtttcgat
    17281 caaatccact tgccgatctt tcaaaaatag gtggccatgc ttatccataa aaccgtaatc
    17341 tccgctatcc caccaatcgc cataaacatt ttcctggaac cgtgcatctt ccttgtaata
    17401 agtcaaagcc cgtcctttag acagtaattg tatatgacca tctgtgtttt caggtaggac
    17461 attcccaact gaatctgtaa tccgagcgct tgtcatgtct tccagtccaa cgcccatatc
    17521 tcttgcatca cttgttttca aactctctag tgtatgagcc ttaagaatca ttggtccgca
    17581 ttcactttgg ccatatactt gaagaaatac cgcatcctgt gccatcgacg ttcgcaaaaa
    17641 tgtcttcatt gtctggttgt ttatagcatc aaaagtggag tgatagtatc ggatacccga
    17701 aaaagcttct ggatgctcct tagcagtaaa acgccactga acaaagttat tcggatgggt
    17761 ctctagtgca attggccggt gagcttccaa catatgaaca acttttgaac tttgtgcgtt
    17821 tgctaaaggc atcataggaa atcccatgga catcaaagaa gagataccaa tattgaatct
    17881 ggaatgcact ggagagatgt gaaaagcgat taatttcttt tccgaaattt ttgtgaaaac
    17941 tgttttttgc catttcgtcc gccatcccat agaatgagca gaatgacaaa taagtttagg
    18001 gataccagtt gtaccagaag tatgggtcat ataagaaatt tcatttttgt ctaaaaatga
    18061 ttgagaaaca gacaaagcag gttgttccat taaataaaga atagg
//
LOCUS       DACDYI010000065        17777 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.065, whole genome shotgun sequence.
ACCESSION   DACDYI010000065 DACDYI010000000
VERSION     DACDYI010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.065"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            227..1192
                     /locus_tag="IWM52_002161"
     CDS             227..1192
                     /locus_tag="IWM52_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP9974391.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(1291..2463)
                     /locus_tag="IWM52_002162"
     CDS             complement(1291..2463)
                     /locus_tag="IWM52_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9974392.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(2450..3595)
                     /locus_tag="IWM52_002163"
     CDS             complement(2450..3595)
                     /locus_tag="IWM52_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9974393.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(3609..4541)
                     /locus_tag="IWM52_002164"
     CDS             complement(3609..4541)
                     /locus_tag="IWM52_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9974394.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMPSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            4658..5737
                     /locus_tag="IWM52_002165"
     CDS             4658..5737
                     /locus_tag="IWM52_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9974395.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            5734..6363
                     /locus_tag="IWM52_002166"
     CDS             5734..6363
                     /locus_tag="IWM52_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9974396.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(6473..6700)
                     /locus_tag="IWM52_002167"
     CDS             complement(6473..6700)
                     /locus_tag="IWM52_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9974397.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(6803..9391)
                     /locus_tag="IWM52_002168"
     CDS             complement(6803..9391)
                     /locus_tag="IWM52_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAP9974398.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(9411..10070)
                     /locus_tag="IWM52_002169"
     CDS             complement(9411..10070)
                     /locus_tag="IWM52_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9974399.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(10119..10622)
                     /gene="trmL"
                     /locus_tag="IWM52_002170"
     CDS             complement(10119..10622)
                     /gene="trmL"
                     /locus_tag="IWM52_002170"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAP9974400.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(10843..11709)
                     /locus_tag="IWM52_002171"
     CDS             complement(10843..11709)
                     /locus_tag="IWM52_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9974401.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            11818..12459
                     /locus_tag="IWM52_002172"
     CDS             11818..12459
                     /locus_tag="IWM52_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAP9974402.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(12521..13882)
                     /gene="mgtE"
                     /locus_tag="IWM52_002173"
     CDS             complement(12521..13882)
                     /gene="mgtE"
                     /locus_tag="IWM52_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAP9974403.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(13900..14799)
                     /locus_tag="IWM52_002174"
     CDS             complement(13900..14799)
                     /locus_tag="IWM52_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP9974404.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(14801..15598)
                     /locus_tag="IWM52_002175"
     CDS             complement(14801..15598)
                     /locus_tag="IWM52_002175"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAP9974405.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(15576..16280)
                     /locus_tag="IWM52_002176"
     CDS             complement(15576..16280)
                     /locus_tag="IWM52_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAP9974406.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            16411..16986
                     /locus_tag="IWM52_002177"
     CDS             16411..16986
                     /locus_tag="IWM52_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAP9974407.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            17104..>17777
                     /locus_tag="IWM52_002178"
     CDS             17104..>17777
                     /locus_tag="IWM52_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP9974408.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLS"
BASE COUNT         5254 a         3813 c         3163 g         5547 t
ORIGIN      
        1 agtaatggat catgcgcttg ctaggctttt ttgtgttttt cggtcttcgg acctttttca
       61 ttttcctaga cttctgctat aataaccaag aattaatttt gtaacagatt tttttgaaaa
      121 aacaagtttc tttcattcaa aaaatagaaa aagcttgcta attattggtt aaccagcaaa
      181 caggagtatt aaaatatctt gttttattga atagcaggag gaaatcatga aattccatta
      241 tcatatattg atcaatccat cggcaggcag cggaaatggc tataaagtag cagaaagaat
      301 tttaccggta ttaaaaaata agcatattga ctatacactt cattatagtg aatacaaagg
      361 acacgatgct gagattgctg aaactttggc aaaagaaaca ttgattcctt ggatagaaga
      421 agaacagaaa acaatcgata ctttccccct tttgatcatc gtaggaggtg acggcacctt
      481 acatcaggta cttgatactt tctatcagat ggaagtagaa ttcccagttg cttatattcc
      541 agcaggttct ggaaatgatt ttgctcgagg ggcaggcctt ccaaaaaatc ccaaaaaagg
      601 acttcagctg attttagcag ctcaatcacc agaaaaagtc cacataatgg cttatgaaga
      661 aaaaataagt gaaaaaaaag gaatagctgt taataatttc ggtataggat tagatgcagc
      721 tatcgtccat gcgacaaatc attcttccac aaaaaagcga ttgaacaaat acaacttagg
      781 atcattttcg tatttattct ctattctccg tgctttattc actcaaaaag gatttccaat
      841 tctggtagat gcaggaggaa aacaattaag tttttcaaac gcatttttat gtacagcaac
      901 gaagcatcct ttttttggcg gaggaatcgc gattgttcca actgctgatg cgtctaaacc
      961 agtcattgat tttgtcgtgg tcgaacgaat caatttcttt aaaatcttgt ggctcattct
     1021 tttacttata cagaaaaaac aattgaaatc gaaatatttc catcactaca caacaaacag
     1081 attacgtatc gtttcaacaa caccgcaaca tggtcaagaa gatggagaag aaatggaaaa
     1141 acgacctttt gatatcacaa taaccacaac taaacagttg ttttggtgtt aataaaagag
     1201 gttttgtcag aagctacatc tggaacaccg tttattcgga aaaaaggact gtgacacgac
     1261 ttttgtcaca gtcctttttt tactatgcag ttaattcttt gcccaattgg gttgccatcg
     1321 atagtttctt tctttaccca aaataaattg gatcattgaa aacaccgcag caaaagcaac
     1381 taagacacaa ccattgaacc aaaaagattc tgcaaatgtt tgattgtaac tgagtgtttc
     1441 actgatcaag ttattatttt gaacaaacca atatgttggt gtgaatgccg ctattttgct
     1501 aaccatttct ggcaagtatt ctgctggtac aaacacccca ccgataaagc aactgcctgt
     1561 gataaaaata tctttgatcc caccgctggt ttctgagttt tttaccaatg aagtgataca
     1621 agccgaaaag ctaatgatcg gcaataaaaa caagaaagta tttaaaatat gataccatac
     1681 cgttatatcc cacgaggtgt ggatcactaa cagaaccatt agaagtaaga agccactgaa
     1741 caacagcagc gagtaactaa tcaaactaaa ggttactttg cgattcaagt ttcgtttggt
     1801 aatgggcgca ctctggtttc gtttactgat ctctttgcga ttaaacgcta gattgatgac
     1861 gctgaaaccg ctaaaaatgg atagaaacaa cccataagac aagaaattaa aggttgtgcc
     1921 cgtcaacagc tttttcatcc gctgactata tcccgcactt agctcaactt ctccagtaac
     1981 cgagagtgcc atttgtgtct gttccatagc tgcatcgatt gttttatctt cttcttgaaa
     2041 cagaatcagt gtttgcaaaa aggtgttcac ttgctgtgta acaactgttt tagaaaaggc
     2101 atctgggcga ctctgtatct tgatcgtcgc catctttcct acttcgactt gttcggtaaa
     2161 gtctgcaggc agatgtaaga taatgtttac acgattaaaa taaagagcat catcgatttc
     2221 tttttgtgag gttccatcca atgtaacgac tgtttccttg tcattcaaat atttacgaag
     2281 gccatcgact acaggcgact cttcttcact aatcaaggcg attttcgcat cttgcagaga
     2341 ggtattttca acatcaatat tttgcgcaaa aaagaatgtg gcaacaacag tgataattac
     2401 acctatcagt aaattcgatt tatttgcttt aagcaatgtc caaatcgttt tataaagcgt
     2461 cataacgcac ccctctttcc atccaagcat ttcctaacgc aaaaaggaaa ataaatccga
     2521 tcaaccatag aagattggta taaaaactag ccaaatcagt gtagaaataa agtttataca
     2581 aactttcgct gatcaagttg acgagattta attgaccaac aatcggaata tgaagatcaa
     2641 tccaatactt cacttgttcc gaccccatca taccagcaaa aaagctcatc gcaatcgtca
     2701 ctgaaatcaa gatgctatcc ttttgcacca gattcatttt tggtatcaaa ttaccaacca
     2761 atgtacctaa caaaatcgca cacaacgacc ccagagcggt aacaatcatc aaccattgcc
     2821 aacgttcacc aaagtctact tgatagataa aacgaaagac agctaacata atataaatct
     2881 ctgtcaaaac aatcgtaaag cttgctgcca aattagcgat tgaaactaac gattgctttc
     2941 taggaatcaa ggacaagcga ataccatttg acgattgatt tgcttgctga tcctgtacat
     3001 tgcggacccc ccacatcgtc ccgtagagaa tcgccattcc aatcagagtg aagaaataaa
     3061 aacttttgaa actcatatta cgacctgcat gaacttcttg aaaagatact tcttgttcaa
     3121 aaacagtcat gatgggcgcg agatcaacgt tctccacttc tgccacttta ctcgaaatta
     3181 taccaatacg ctgtaaaaat tgatccatga cgtttttcat caacgtctga ctcattcctg
     3241 cttgacccac atggagcgaa acagatgaac catcaatgag ataaaaacca gccaaatcat
     3301 ctgcatttag cttttcattc gcttctactt ccgatagttc ctgtacttca aataatggtt
     3361 tatcattgac ttcgacttgt tctaagactg tggtaaaatt ttccgaaagc gctgtttgat
     3421 ccgctttgat tacaattcca gtattaatcg tttcaagatt gccaccttga tcaatctcgc
     3481 caaaggccgc cataaacatc aatcctaaaa gaattggaaa ggccaatgtc caaaacaaca
     3541 tggatttatt tcgcagcagt atttttgtgc gatatttata aaaatgccaa aacattattt
     3601 cccctcctct agtctctcaa atccttgcca gtaatctcta aaaagacatc attcaaggac
     3661 gcttctttgg ttgctatttt tccgtaactg atcttttctt cttgtaagat tgtaagaatc
     3721 ggcaacaata ctgtatttag ttcagtagtt gttacttgga gctgaccttt tttataagcg
     3781 attccatcag aagctattgt tcgctgaatc gctgacaaca cattttctgg gagtaatggg
     3841 atgtcgatcg tcaaacgatc cgccgtccgt accatatctt ttagagattc tttggttcct
     3901 ttagcaatca cctgtccttg atccatgatc acgatctggt cgcaaagaat ttctacttct
     3961 tccatgtaat gtgttgtata gacaatggtt gctccttgtc gattcagctc cttgatactt
     4021 tctaaaattt tattccgact ttgggggtct acagctactg tcggctcatc taaaaaaatt
     4081 aaccgcggct tgtgggcaat cccacatgcg atgttcaacc gacgtttcaa gccaccacta
     4141 agttctcctg gacgaaacga aacatatttt tctaatccca ctaattgaat cacctcttca
     4201 atcaaggctt tccttttctc tttttcggaa acatataaac cgcaaaaata atcgatattt
     4261 tcttgcacac ttagttcgtc aaagaccgca acgtcttgag gaacgacccc aatcattgcc
     4321 ttgacagcat aattttcagg agtcattgtt gtcccgaaca atttaatggt tcctttgtca
     4381 taatttaaca aagaaggcat acagttgatg gcagtcgatt tcccactgcc gtttggacct
     4441 aacagaccta agacttctcc ttcttgtact tgcagatcaa aatgattcaa tgctacaaaa
     4501 tctccatagc gtttgactaa tttatcaatt tcgataatca tcatgaaaaa ctccttccaa
     4561 atttcttgtt gatctgacta taataaagtg caagaaaaga aactagtgac gattggctaa
     4621 accacacatg acatttgtca caatgaagga gtctgctatg cataaatctc ggctactcaa
     4681 cgaaactagc tttctactgg ttatctcatt gcttttgctg gtaagtcaag gattcgttgc
     4741 tatgcaagtt atctatttat tgatcgccgt tttattcact ggtgtcagtt atttactgcc
     4801 tacaaagcca aattggattt tccaagttat tctatttctt ttatgtgcct ttttacctga
     4861 tcttgtcctg tttctgccag cgacgatgcg agtgatcgcc cctgaagaaa gccaccttga
     4921 ttacttgttt ttgttaggtt ttttgttgat ttcgacggtt ggtaatcatc cccccatcaa
     4981 ggtcgccttt gtcgtaattt tgctgttttt ttccttttac ttacgaaaaa aagatcaacg
     5041 aacgaccgat cttgagaaaa agtacattgt ctccaaagat actcattggg aaaaacaaca
     5101 agagttatcg gaacaaaata aagcattggc cctgtcacaa gaaacacaat tagccttgca
     5161 aattgtggaa gaacggaacc gaattgcccg agacatccat gacaatgtcg gtcatctgct
     5221 gtcaagtgcg attttacaaa ttggtgcgct tgaaatgatc aatcaagaac caaaactaaa
     5281 agaaccatta gaaaatttaa gtgccaccgt tcacactggg atggatcgga ttcgtcaaag
     5341 tgtccatgac ctccatgaga catcgatctc ttttcaacac tccttagagt ttctgatcgc
     5401 tgattttcct tcttctgtta cgattgaagg aaacttgttt gaaggattga atgaaacgca
     5461 gcaaaactgc ttcttaatgg tcatcaaaga agccttaacc aacagtatca aacatagtca
     5521 tgccaaaacc gttgttttac attttcgaac gttgccggcc ttttatcgct tgcaaatcac
     5581 gaatgatggc accagcccac aaaaaaattc tgctcccggt attggtttga aaacgatgcg
     5641 tcaacgtgtt caagaacaaa agggacaagt ccatattttc caagaaaacc agcaatttca
     5701 gttaacgatc attttaccaa gggagggaca ccaatgataa cagtagtcat tattgatgat
     5761 gatccatttg tgacgaaatc gttacaaacc attttagaaa gcacgaaaga gatccgtgtg
     5821 ttaggcattg gtcattgtgc caaggatgcg ctagtcttat acgaaaccca tcgtcccgat
     5881 gttttgctga cggacatccg catgcctaaa caaacgggca tcgatgctgc caaagaaatt
     5941 ttggctaaat ttcccaaagc catccttttg cttttgacga cattcaaaga tgaagaatac
     6001 atccgcgaag cattttccat cggtgtcaaa gggtatttaa tcaaacagaa cttgcaagcg
     6061 attattcctt ctgtcaaatc cgcttataat ggacaggccg tatttggtaa tgaaatcatc
     6121 gagactttca ctcaaatgat gaagaataaa ccgacgattg atagtcatgc ttatgcttct
     6181 tttagtgaac gtgagcttgc aatcatcaaa gaagtagctg ccgggaaaaa caataaagaa
     6241 attgcagatg ccttgtactt gagtgacggc actgttcgca attacatcag ccaattacta
     6301 gaaaagctag atttacggga tcgcacgcaa ttggctatct atttttatca gcatttgcaa
     6361 taagtcttat ctaacacaaa aaaacctgaa tgatgctcct tcgcatcatt caggtttttt
     6421 tcacatcttc tattcaattg tcccgcaaac catttcctca cgattgaaag aatcaaggat
     6481 tcgcagggct gccaggattc gttggattgt tcggcgtgtt aggcggaaaa ataggcgcct
     6541 tgtctaacgt tttttctagt tttgattctt cacgatcgtc tcgttctaca tcaggacttt
     6601 cattcaattt gaattctttt tccatctcat ctaacttttt aaagtcattg ccagattctt
     6661 ttgctaattt cgctaattta tcttcggttt ctttagccat aaagtaacgc cccccttttg
     6721 atttcagctt agcatatcac actgaaaaaa caaaagaaaa cgctaaccaa cgatcatttt
     6781 tagctatttc tcatctttaa aatcaagcat tttttgccgg tataaaatcg tagggagaac
     6841 tgttttccat accaatcatc ggatctacta actggttatc gatcacttct gctgtaagaa
     6901 cgaaatcttt agaaacagca ggttctacct cttctttctt ttcttctcca acaaatgaag
     6961 aaaacagatc tggttccact gtttccatta cttggttttt cgtttcattc gtcccactgt
     7021 catcttctac aggtagcttt tcttttacaa cccgtggctc ttttgtatag ttcccatctt
     7081 caaatggatc atttccgacc aaagaatctt cataagctgc tagtttggtc cggagagtga
     7141 tattcatttt atcattatcc atgatccttg atttcaacgt agtcatccgg cttgctgatt
     7201 cattcgctac acaagtgaca taggcttgct cttcccctaa caaaatcaac agttccttac
     7261 tgcgagtcac tgccgtgtag agcaaattac gctgaagcat ccgctgatat tgatggacca
     7321 tgggcaaaat aaccatcttg aattcactgc cttgtgattt atggattgag caacaataag
     7381 aaagagtgat tttattccat tcgtttcttt tataagtgac ctcatttgca tcaaactgga
     7441 taactaattc atccacttta tcttcagaat cttttgccaa gatgataccc gtgatttctc
     7501 ccatatctcc attgaataca ttcaattcgg gagtattgac taattggagg actttgtctc
     7561 ctatccgata aacggtgtcg ttccaatgta cttccttttt tccaggtttc cctggattga
     7621 atatctcctg catcatttta tttaacgcat cgatccctgc aggtccccga tacattggtg
     7681 ctaatacttg gatatcttgt gcacggaatc ccttattttt cgctttttcc acaattttag
     7741 agatcaaggg ttcaatttga taagcatcac ttcggaaata ggaacgatct ttttgattct
     7801 ttgtgaaatc ttttggcaga cgtccttctt tgatctcatg ggcaagagga ataatactag
     7861 aaccatcacc ttgccgataa atctgattga gttccatttt gggaatttgt tcgatttcca
     7921 aaagatcatg gagtacttga ccaggaccga cagagggaag ctgatctttg tctcctacta
     7981 agataacttg catattcgtc ggaatcgctt tgaataatgt gttagccagc catgtatcga
     8041 ccatagacat ctcatcaaca atcaaaagtc cgccttctaa ttctttcgta ctgactgaag
     8101 gtgttttttc ccgtccattc agccccagaa gacgatggat cgtactggca ggcagacctg
     8161 tcgtttcgtt cattcgttta gctgctcggc cagttggtgc tgcaagtaga ataggaaaaa
     8221 tttcttgggt atattttgaa ggatccaatt ccagtccgtt caactctgca aaaagattga
     8281 cgattccgtt gatcacagta gttttccccg tacctggtcc tcctgttaaa ataaatagag
     8341 gggaacggat tgcttcagcg atagcttgtt cttgcgaagg tccatactgt atgccgaata
     8401 ttttttcgat acgccgaata ttcttctgca cttctttttc aggataatgg atttcttttt
     8461 tacgactgag aaggcgctgg atcgaattac cgatccccca ttcagaaaag tacaaactgt
     8521 tttcgtaaag attagtctct tcttgttgta tctttccttc ttcaacaagt tggataatta
     8581 cagtagcaac ctgatcaggc tggatttcaa ttgggcgact tgtttctaaa atacggatcg
     8641 tttgctgaag caatgtctcc gccggtacgt atgtatctcc tgtttgaact gcatgctgaa
     8701 aaatctgatg caagatagct gctcgaattc ttctcggtga atctgctgca ataccgattt
     8761 gttcggctag attatctgca cgtttgaacc cgattccttc tatgtcttcg actagctggt
     8821 acggattttc ttcgatgata tccaaagact cgttcttata ggcctggtag atactgaaag
     8881 ctaactgact accgaacccg taacgattca gtcccatgat cacttgatcc atcccgtggt
     8941 ttagccgaat cgtctcagca agcatttctc tttttggctt cgtcaaaccg gagatttgtt
     9001 ttaataccga aggatcagca atgatttttt cgatagcatc ttctcccagc aattctacaa
     9061 ttttttctgc tgttttcttc ccgattcctg ggaatttttc gctggaaaga tacgctacga
     9121 tcccatttgc agttgttggc ttttcttgct catatctttc gagtttaagc tgccttccgt
     9181 atttaggatg atccactaat tctccataaa aacgataagt ctcattttct tgaatatcgc
     9241 cgaaacttcc ggtgactaca atctcttttt cacggtaatc agctgtggtt tcactgatgt
     9301 gtaccaaaag cactttgtaa aaattactag gattttgaaa aaaaatcgcg gcaactgtac
     9361 caacgaaata aggcatttct tctggctgca tcggaagccg cctccttctt ctatagtaaa
     9421 ggttctggtg ctgtttcagt tcctaaaaaa tctgtttggt tccatacttt cagttcatat
     9481 ggcaataatc tgcaaggctc ttcagtctct aaaatcgtca aactactatt gaacaatcca
     9541 cccatttcac gcaactcact tagctctttt cctgtcatcc attgaatggc tgcagttaaa
     9601 gaagctccat gtcctacgaa taaaagtggg ccatctttat gtcgggcaac cgcatctgct
     9661 acaacagttt cgatacgtgt gattgccgat tcaatcgttt ctccgtgaaa aggagaagga
     9721 tcatacagat ctaaatgatt acgcaaattc ggcagattat ccggatattt ctggtacatt
     9781 tcatcgatcg actgaccttc taaagtaccc agccctagtt ctcttagctt atctgtataa
     9841 aaaatctcta ccggacgagc aagctctttg ttgatacctt ctgctgtatc acgtgctcgc
     9901 tttgctgtac tggaataaat tgctttaaaa ggaacatatt ttaccttttt cccaaatgct
     9961 ttgatttctt cgtaactttg tggcaaaagc ggagaatcgc cattgctccc ttgaaaacgt
    10021 ctttcctgat tccattgtgt cttcccatgt cgggtaaaat atagttccat acgcagcaaa
    10081 ctcctgtctt tcttttattt tactctataa taaactgtct aatccagctt gtcattttca
    10141 tagtgatgcg tcaattctaa attagggaat ccttgctggc gaagtgcttc atacactatc
    10201 attgcagctg tattcgataa attcaatgag cggacatgct catcattcat agggatgcgt
    10261 aaacactttt cttcgttttc acgcataaat gtttctggca aaccggttgt ttcttttcca
    10321 aataaaaaga agtgctcttc tccatcagca aaattttcgt cactatatac tttatgggca
    10381 aattttgaga tcaaatgtag tttacgatta cctaaaaatg ttaaaaaagc ttctaaatta
    10441 ctgtggtaag taatatttac atcattccaa taatccaacc ctgcccgttt tagatgtttg
    10501 tcatcagttg aaaaacctag tggctcgatt aaatgaagcg gagtattcgt ggctgcacac
    10561 gtacgggcaa tatttccagt gtttgcaggg attaatggtt caaataaaac gatatgattc
    10621 atcaggatca ttcctctcat atttatgttc gtttctattt tctgacaatt tgatttttgc
    10681 ataaaaaaaa acgtatcaac tcggtgtcag tcactgcgag tcgttacgtt agtgaagctc
    10741 ttctcactaa cttttatcag aacaggaacc tgataaaaac caatgaaaaa acaacttgtt
    10801 gctaatataa caccctcaac ttttttttgc aacctctact cattaaaaac tgctagaaaa
    10861 acgttttctc ttagttcctt ttaataattc tatgtcaatt gtaatcttct tcaaaggatt
    10921 cttatataag tctgctttga ttcgctcttc tacctgccat tctaaaggtg acatggccaa
    10981 aagatctttt tgattttcct ctggtatctc aaattcatag gttacacgct gacgttcagt
    11041 taatttcatt gcttcagcaa acttggctac tacacgttca ttggaataac tctgtttttc
    11101 aggttgattc ggataaaaag cctgacgtag ttcctttaaa taattttctt gtggcaccac
    11161 cttcaacact tctccatctt ctgagagtac acgttggaat tcttggtaat gagaaggcga
    11221 aaagatattt aaaaccgcgt tgaatgtttg atcagcaaac ggcaaattgg tcaaatcagc
    11281 tacacaccaa aatgcttcgg cagattgttc tgtagctgcg tagactcctt cttttgaaat
    11341 atcaaatcca acagcatttt gaatcttttt caattttgaa agttcattca agaagctacc
    11401 ttctccgcag ccaacatcta atagcgagac ttcttcaggt aaatgctgcg caatagtctc
    11461 aataacgggc tgatacatgc cagattggat cattctacgc cttggaacga acattccttg
    11521 atcgtattct gttttaaccg catgggataa aaaataaagt gttccttttt tggaaaggtc
    11581 aaaacggtga ttatttttgc atatcaagcc attatcagac ttttttactt cttcttggca
    11641 aaccggacag cggaacagct gatgattttc tgccaaaaac tggcgtcctc ggtcaatttt
    11701 ttttaacata gttccccctc atttctgttt tatcttaccg ttagatgctc actttggcaa
    11761 ctgctaaaaa tatgatacaa tgcctagaag aaagtgaaag ataaaggaga agattagatg
    11821 atcaaagaat tttgcgctga aaactacaca tctattcctc tagcaattgc tagaggagcc
    11881 gatcggatcg aattgtgtga caacttagcg gtcggtggca caaccccgag tactggtgtg
    11941 atcgaagaaa cgttaagtta tgctggggaa aaagatatcc ccgtaatgac gattattcgt
    12001 cctagagggg gcgacttcgt ctataacgat atcgaattga aaatcatgca tactgatttg
    12061 attgaagcaa agaaattagg gacggatggc gtagtatttg gttgtcttac tccttcaggc
    12121 tggctagacg aagaggcctt agaactattg atcgaaacag cagaagggtt gcagatcacc
    12181 ttccatatgg cttttgacag tattcctgaa gatcggcaat tcgaagcaat cgattggctg
    12241 gtagaacatg gtgttgaccg tatcttgact catggcggtc ctgctgacaa aactattgta
    12301 gaaaatttcc cacggcttaa agaattgatt gcctatacag atggccgctt aatcattttg
    12361 cctggtggtg gtgtcactgc agagaatgca gcacatgtag ctaaagaatt gaacgtaagc
    12421 gaagtacacg gaaccaaaat cgtggatctt cagttataat cttataaaca aaaaaacatt
    12481 tgcaacaggc ataatgctta ttgcaaatgt ttttgttttt ttacataaaa tagcccataa
    12541 ataaagaagc aatattgaaa taaatcagta cactcgtcaa atcgctcaaa gtcgtgataa
    12601 agggaccgct tgctactgct ggatcaaatc ctagacgatc cattagcatc gggattaaac
    12661 tacctgcaag attcgccact gtgattgcaa atagcatagc catccctatg acaaacccca
    12721 agataaaatt atgtttccaa aaacctacga ttagaaaaat cgtgatcccc gtaacagctc
    12781 ctgtgacaac gccggtcaag acttcagcca aaacaagtcg aacaaatcca tgatctttct
    12841 catcagaaat cgcaagtcga cgaacagcca cagctagcga ctgggttcca gcattaccgg
    12901 cagttcctgt aatcagcgaa atgaagactg ctaaaatact cgcttcactt accaagtctt
    12961 catagtgact gattaaagaa gcggtcgaca tacctaaaaa caacagagtg atcagccaag
    13021 gcaaacgtcg agacgctgcc tttactggat tttcattaat ttcttccaca ttgaccccgg
    13081 caagtcctga ataatcgcta gcagcttcat catcaataac gtcgatgata tcatcgactg
    13141 tgacgattcc taacaaatga tcatcataat cggtaacagg aagagctaag aagtcataat
    13201 ctctaaatgt ttgtgccaca tcttcttggt cgtcgcctac atgaactgaa agcactcgtt
    13261 cgctcatcag atcagaaatc attgcgtcgt catcattcac gattaggtcg cgcagagaaa
    13321 taacccctac tagctgacct tcttgattaa cgacatagat gtaatagatt gtttcagcta
    13381 catctgcttc gttcttcagc acgtacatcg cagagcgaac agtttgattg gctacgatcg
    13441 aaacaaattc agttgtcatg atcgcgccag ccgtttcatc ctcgtaatgg agaagttcct
    13501 tgatttcact ggcatcgtct gccgacatca aacttaaata tttagcaatt tgctttttat
    13561 ccagcgtatt aagcaagtca acagcattat ctgtatacat ctcagaaagc atatcagctg
    13621 catagctagg acgcatttca tctaggtatt ctttcatatg ttcgttgtct tcttcaatca
    13681 catcgaacat atccgctaat tctttaggcg acagatagga atagatcagc tgcctgttct
    13741 cttctgtaag agattggtag aattgcccct gctcatagat atgcatctct aaaaaactgt
    13801 ttctgaatgc ctgaatatta ttttccctta aatcttgagt caactgggca aatctttcct
    13861 ctaattcttg attttcatcc aaaccaatct cacctcgcct tattcttcaa accgtctcac
    13921 aactgcctcc atgtctgcgg ctaaaggctg cttgaactcc agcaactctt ctgtaaatgg
    13981 atggtaaaaa cgcagatcat aacaatgaag ggcttgtctt tttattccca gatccattcg
    14041 cccgccatac atatcatcac cgagcagggc acagccaatt gcggaaaaat gaactcttat
    14101 ttgatgtgtc cttcctgtat gcagctggat atcaactaac gcgatttctg ttagtcgctt
    14161 ctctatccag tactcagtct gtgccgaacg tccttcatca gagaccattc gcttcaatag
    14221 cgaagaataa tctcgggcga tcggcaaatc gatatgtcca tgtgccgata aatctgaaag
    14281 ccttccactg actaaggcct gatatttttt cacaaacttc ttttcacgaa gttgcatatc
    14341 taattttgcg tgcgcaaatc catgctttgc aaaaagcatc aagccagatg tatcccgatc
    14401 tagtcgcgtc accacatgga ttacttggtt ttcgtaacct tgttttttat aataagcctt
    14461 tacacgatta gccattgtcc catttggatg gtattgggca ggaatagacg aaacgcctgc
    14521 tggtttattt actaccagaa cgtgttcatc ttcatacacg atatcgatcg gtgtttcatc
    14581 caaaagtacg gtttcatgtt ctccttcagc aggaatcaca atggttacct tatcatcctt
    14641 agctagagaa aacaagacat tttctacttg atcattaact ttgatttgac cgccttgaaa
    14701 ttttattttc gctaacagac cctttgagat ccctttttct tttaaaaaat attttacttg
    14761 ctgagaatga tctttctcat aatcccatgt aaactgcata tcaatcctca ctaataaagg
    14821 catctttcac acgatgccaa aaatgcatat gtcgataaga agcaaaatgg atccgttctt
    14881 ctgctattcg ataataaatt gagcggatat ttgcttttgc gacattcaat tgatcaaccg
    14941 ttacaagata atcttctgta ttttccaatt ttatttctag ccattcatca tgggcaatca
    15001 cgatcggaga acccaaagta cgaaacactc gattattcag tgaagcaatc tctgccaact
    15061 gaaaggcatt gatactagga tggatcacag caccgccgac acttttatta tatgctgtcg
    15121 aaccagtagg cgtagaaatc gtcaatccat ctcctctaaa actttcaaac agctcgtttt
    15181 tgatgtatac atcggctacc atcgtacgat tggcccgttt aatcgttgat tcattcagtg
    15241 ctaaaaaatg ctggtccggt gtttcatcca aataactgat ccgaacatcc agcaagggat
    15301 aactaacact ttgttcccgg ttcgttcgta agctgtctac tagttcttcc agctcgtaat
    15361 cccgccaatc cgtgtaaaac cctaaatgac ctgtatgcac gcccaagaaa cgcacatcat
    15421 tcagacaatg actgtagtga tggaacgccg acaataatgt gccgtctccg ccgactgaga
    15481 tgaccaattc tggctcatga tcatctcgtt gaatccctgc ttgttctaaa agtgcgttca
    15541 aacgttctgt cacttctata gtctttgctt ctttattatg aacgatcgct actttcatcc
    15601 cctgcctcct ctctttcgct ttctttcttc aaaagcgttc gatagtagac atcatctgat
    15661 tttcctcttc catgggagaa taaccgctgt gcttcttgga tttcttcacg gatactggac
    15721 atctcttcat ccaactgata ggcagcttct gccgcacgct gaagtcgttc actcatttct
    15781 tctggaaaaa cgccttgata tttataattc agcgaatgct caatcgttgc ccagaaattc
    15841 atcgcaagag tccgaatctg gatttccgcc aaaattttct tctcaccgct gatcagctgt
    15901 acaggatact cgatcaccag atgataagaa cggtagccac ttgctttttt atttgtgatg
    15961 taatctcgtt cttggagaac tttcatgtct tttcttttac ggatgatctc aacaacttga
    16021 tggatatctt ctacaaactg acacatgatc cttaacccgg caatatcaga catttcttct
    16081 tctaaacgtc ccattggaat atgccgtaat tgtgattttg ttacaatact atcgactggc
    16141 ttgactctcc cagtgacaaa ttcaatcggg acatgtcgat tttgttcacg gaactgtttg
    16201 cggatccccc gcagtttcac tttcaattct gatacagttt gttcatatgg tgctaaaaat
    16261 gcttcccaat ctcgttccat agctgatacc tctacttcat taacattcat tgtttattct
    16321 accatatatg caaagttttt tcgtactctt gatttatgca ttgaccaaaa aaagaaaatt
    16381 aggcaaaata gacaaaaagg agtcgatcaa atgacacaaa tgcttgaaat cgaatataaa
    16441 tctatgctga caaagcaaga gtacgagata ttcatcgctc actatcaatt aacagataaa
    16501 gatttccgtg tacagactaa tatttatttc gataccgttg atgaacagct gaagaaacaa
    16561 aattgtggcc tacggatccg tttcgttggt catcaagctg aatatacatt aaaaacacct
    16621 gctgaaaaag ggcgtctcga aacaacggat acctttataa cagaagaagc agaagcattt
    16681 atcttcgaaa aacgtctgcc tcgttcaggc gcagtttttc aaaaactttc tgatttgaat
    16741 atcgatcctg cttcattaat ccaaaccgga aagcttacaa caaaacgagc tgaattccct
    16801 attgaagaag gacttttagc tattgatgaa agctggggag acaatctaca cgattttgaa
    16861 ctggaactag aggtagggga tgcatctgtg ggaaaaattg cttttataaa ttttttaaaa
    16921 cgtttttctt taccttatcg tcctgctaag aacaagatcc aacgtatgct tgaagctaaa
    16981 aattagaaac ctttcatttt cacaaggcac ttgagttcat ttttttactt ttttctgtta
    17041 agttatagga tgtgaaagaa tctagtcact agttgtatag aatctatttt gggggagatt
    17101 catatgatcg aaatctattt atttgttaat cctttagggg aatactgttt cgaaatggaa
    17161 caacagttat tacaatttat cgaagaagag tacgggacac catcaaaaga aaagatgcaa
    17221 tttcgctttc ttcctttggt caatttgcag acaattgggg atgtcatgca acaaaaagga
    17281 atttctcaga acaatcttga ggaacgtaat cacttattct caatgactta ttctgcagca
    17341 ttggactgca aagcggcgca gtttcaagga aagaaaaaag gccgccgttt tttgatcaaa
    17401 ttgcaagaag cagtcggctg taatgacata ccatattctc aagaacttgc tgaatccata
    17461 tttgaagaaa tcggcggcga tttgaatatg tttaaagaag atcgtcagtc tgacttcgtt
    17521 aaagaagtat tcttttctga tcaaaaaatt gctcgggaaa tgggcatcac aaaacaccct
    17581 tctgcagtcg tttacaatta tgcctgcgac cgtgattttg gtgttttagt agaagtaaaa
    17641 ggtacaagta gcctttggag aatcacagag ctgtgtaaaa caacagataa ctcatatcaa
    17701 gtttttaatg gtgaacattt acatacaaat gattctcagt tccattcaag aactagccat
    17761 ttacacttgc tttctaa
//
LOCUS       DACDYI010000066        17673 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.066, whole genome shotgun sequence.
ACCESSION   DACDYI010000066 DACDYI010000000
VERSION     DACDYI010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.066"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..199)
                     /locus_tag="IWM52_002179"
     CDS             complement(<1..199)
                     /locus_tag="IWM52_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974409.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
     gene            complement(362..1996)
                     /locus_tag="IWM52_002180"
     CDS             complement(362..1996)
                     /locus_tag="IWM52_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9974410.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTSLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            complement(2000..3373)
                     /locus_tag="IWM52_002181"
     CDS             complement(2000..3373)
                     /locus_tag="IWM52_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9974411.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            complement(3389..3628)
                     /locus_tag="IWM52_002182"
     CDS             complement(3389..3628)
                     /locus_tag="IWM52_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974412.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            complement(3810..6485)
                     /locus_tag="IWM52_002183"
     CDS             complement(3810..6485)
                     /locus_tag="IWM52_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HAP9974413.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            complement(6668..7591)
                     /locus_tag="IWM52_002184"
     CDS             complement(6668..7591)
                     /locus_tag="IWM52_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974414.1"
                     /translation="MATNYAKKINFDVDKLYKSLIVVPKNKSEDGNYELEVLQFKIEQ
                     KSSERFMSNQLVKIKKWLPMIDQLVSNNKEAKNLCIFVLLLHGRVINLVNRKSTTKFA
                     NSFWFLKNINKTVEKNGVYKGDFHFQNAETIEREHHYFDLLVNTVVELAKWAKSNETY
                     SQVLKRIMSQETSFIYELSKSNLKYTYNEDELEILDNPENSQWIKELKREDFIETLRI
                     LKSLFDLKLAKATIMGIERDSYYWEEIGLYAPQIEGEDKEKRIDNIINYINSLSEPLK
                     CRGLDFIGTLGWTETYYEKIYKEIIEQYFPK"
     gene            complement(8114..9118)
                     /locus_tag="IWM52_002185"
     CDS             complement(8114..9118)
                     /locus_tag="IWM52_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP9974415.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKETVETDTTIEAKKSKTQS
                     TEQTTTEMKQSVEASTSESSIIESSSEEVKNTLWDSEKSTQLAIFVPQWGKTLGQEYK
                     SYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVET
                     GEYLGQHVYFFGFQNGQPKVYLTQQNQGNDKNYMYFNETQNQQLKQGFIDIVNGQTPQ
                     TPVVEESTQQATTTTTQSTSSSAISSWEAAKETVLNNKALWLYDSPDKNASNTNLIST
                     NEYMKDLMQDENGSYYSINIVTDDNGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK
                     "
     gene            complement(9189..9485)
                     /locus_tag="IWM52_002186"
     CDS             complement(9189..9485)
                     /locus_tag="IWM52_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974416.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHAGYRHSWERIN"
     gene            complement(9478..9771)
                     /locus_tag="IWM52_002187"
     CDS             complement(9478..9771)
                     /locus_tag="IWM52_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974417.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            complement(9917..10468)
                     /locus_tag="IWM52_002188"
     CDS             complement(9917..10468)
                     /locus_tag="IWM52_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HAP9974418.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFPRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            complement(10642..10941)
                     /locus_tag="IWM52_002189"
     CDS             complement(10642..10941)
                     /locus_tag="IWM52_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HAP9974419.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGMLPEDVT
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTNE"
     gene            complement(10968..11276)
                     /locus_tag="IWM52_002190"
     CDS             complement(10968..11276)
                     /locus_tag="IWM52_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP9974420.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQFHLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEARKEYLISPKLRLKSVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            complement(11393..12352)
                     /locus_tag="IWM52_002191"
     CDS             complement(11393..12352)
                     /locus_tag="IWM52_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9974421.1"
                     /translation="MTYTHLTTDELVIIESYFNIEKPVALVAQSLNRSRQTIYNVYSF
                     LKQGKKVLDYYRQYKQNKSRCGRRSTVLSDEQSTYVQEKVAQGWSPDVIIGRGEFPID
                     CSVRTLYRMFKKGLFDTQSLPMKGKRKPNNHQEKRGKQAFRRTIHERNSLYPHLTKEF
                     GHLEVDTIVGVKHKSAVITLVECLSKVIITLKPTGRRAEDIENRLNDWFKSMPKHLFK
                     SMTFDCGKEFSNWRSISNSNDIAIFFADPGTPSQRGLNEHSNGLLRRDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            12496..13272
                     /locus_tag="IWM52_002192"
     CDS             12496..13272
                     /locus_tag="IWM52_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9974422.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLAESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            complement(13370..14017)
                     /locus_tag="IWM52_002193"
     CDS             complement(13370..14017)
                     /locus_tag="IWM52_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9974423.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(14017..14847)
                     /locus_tag="IWM52_002194"
     CDS             complement(14017..14847)
                     /locus_tag="IWM52_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9974424.1"
                     /translation="MENSTEKKRLITKKDLNKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTSFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            complement(14847..15596)
                     /locus_tag="IWM52_002195"
     CDS             complement(14847..15596)
                     /locus_tag="IWM52_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9974425.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            complement(15610..16074)
                     /locus_tag="IWM52_002196"
     CDS             complement(15610..16074)
                     /locus_tag="IWM52_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9974426.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            complement(16124..16585)
                     /locus_tag="IWM52_002197"
     CDS             complement(16124..16585)
                     /locus_tag="IWM52_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP9974427.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            complement(16567..17541)
                     /locus_tag="IWM52_002198"
     CDS             complement(16567..17541)
                     /locus_tag="IWM52_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9974428.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
BASE COUNT         5414 a         3376 c         2580 g         6303 t
ORIGIN      
        1 tataataaac atttccttga ggtttccttt taatcccctt cggaaaatca ccttttgtaa
       61 aatcgctctt aaggaaatca tctaaattac cagttctggt ctttttacct ccagtattcc
      121 ctgatttttt aggtggtaca tgttctttag taagttcagt gtatttttca cataaaatac
      181 acttacccat ctttttcata tctatcccca ctctttttaa ttcttatata caattatatt
      241 tcaaatattc attaataaat atccctattt atgataagga cctccctaac tacttctcta
      301 acgatttcaa atcgctagag aagcgttagc gcccctctaa cgttgtttga tataacctag
      361 actagatcgt taacgtaaag ggtgttttta acacaaaaat agccgtatcc tttgttttgg
      421 atacggctat ttgatattgc tcattcaatt gttgtaccac ggcatgcgtg aatttcatcg
      481 taatttccaa catctcctta tggttaggat caaaggttat tttctctata aaggtacgat
      541 acaaggcttt gatttgcttc ttctcactct gggcaagaaa acgatccaaa ctagtcaata
      601 tccgttggac atctttgact tgaatcggct ctccttgttg aaagatccct aagatttcat
      661 tttctcgaat gtgcaactgt cgtcgttttt cagtcaattc gtctaagcgg tcttttaaca
      721 tcggaaacaa ttcaggacta tcttccaaag cagcttccca tttttcaatt ttagtcttca
      781 attcttcctt tcgttccagt aacaccacga gttcttgttc caatggttgg ctttgtcgca
      841 ctatttcttc atttaacgct gccaccaatc ggggaagaac ttctggtagc tgaacgatct
      901 cctttaatcg gtcggcgaca aatgtttccg cttgttcggc acgaatcgaa ttggcatgac
      961 acacacttgc tcctttattc cgaaagactg aacaagaata atatcgaatc cgtttctttg
     1021 tgccatcctt tagggtattt gtgacattac tcgctgccat cggtgcgcca cattctggac
     1081 aacgtaacaa gcctgtcaat acattttcac ctgtatggtt ccatttgggt tgttttcctt
     1141 ctaaagcaag gcgttcttgt actttttgat aatcttcttc cgaaataatc ggttcatggg
     1201 ttcctttcac taaaatcggt ctcggattct tccccttccg tcgtttggtt tcccaattga
     1261 tgtagcgcgc atattctaaa taccctgcat aaattttatt gcgtaagata tctttgactg
     1321 ccgtggtaga aaaagcattc tttttgactg tttgatatcc ttgacgattc aaaaaattgg
     1381 caatcgctcg ataccctgct ccgcctaaat attttgcata gatcagacga acaattttcg
     1441 cctcttctgg ttcaatgacc aattgttttt gaccattcgt gtcaatacca ctgacatagc
     1501 ctaaacgtcg gcctcctgca taacgctttt tatctttcac taagctttgc attgacattt
     1561 gaacattctc cgcaatttgg ttgcgttcaa attcaccgaa actcccaagg agttgtaaca
     1621 tcaatttacc tgtcgaagtg gtcacgtcaa attgttccga gatcgaatgt aatcctacgt
     1681 catatcgcaa taattcctcg actgtttgta agacatctaa catattgcgt ccgagacggg
     1741 tcaatttcca aatcactagt agatcaaaac gatggttccg agcatcgttc atcattcgtt
     1801 gataggcgaa ccgatttttt gtggatttcc cactgattcc ttcatcggca taaacttcta
     1861 ccacttcata gcccatccgt tcggcaaact gtcgtccatt ttgttcttgt gcttgaatac
     1921 tatgtccttc taaagcttgt tcttgtgtac tcacgcgaat atacaatgcg actcgtttgg
     1981 tttgtatcat ttccatagat cattcgttcc tttctaaaat attttgtgtc agttgtcgga
     2041 agtaaatccc acaaactgtt ttatcctttg ttacctcaat ccgacaaaca tagtcacata
     2101 ataattgtcg actagagagg tcttttagtt gaagccattc tttcactttt gcttcacact
     2161 gttggagtga aagaaacgaa gaagtagaca tcggcttttg aagagcttgc agttgttgct
     2221 tgagttcttc cagtgaacaa ttgccctctt caaatgcctt aatccattgt gcttcttgtt
     2281 tcttttgttg gattactttg cgtttttcat cttttcgctt ttcggctaat tgttgcgtta
     2341 attgggcaat caaggcttga tagacgcctt cgtattgaaa aaaagcctgt atcttttcca
     2401 atacttctga ctcaaccctt tctgcccgaa gaaaaattcc ttggcagctt cggtcggtac
     2461 aatgataata atgaagcgtc ttcgtcttct ttgtattcca ttgatacttc gaactcagat
     2521 gacgaccaca gaagggacac cgaatttttt gacgtaataa gtacagatac gtatcttttt
     2581 tagctgtaca ccttgattgg cgaatctctt gggcttgtaa aaaggttgct ttcgagagaa
     2641 tcggttcaaa ggttcctaga tacttcccca aagatcctcc tttgatttcg ccatagtaca
     2701 atggattttt taggatacta taaatatgat agggctgaaa aggacgttcg cggaagaaaa
     2761 gcttttgttg cgtcaatcgt tgacaaagct ttttgtagcc atattttttg gtcaaataaa
     2821 gttgaaaaat atagcgaaca atccttgctt cgtgtacttc ttgttggatt ttcccattca
     2881 ctaaacgata cccatatggt accgcagaag acaaatattg ccgttgttgc tttttatagg
     2941 aaagtccggc acgaacattc tcaataatga cgtttcgttg ttgttctgca aaagtgaaca
     3001 ataattgcac aaatagttta ttcgttgacg cgtgtgagaa agaagcagga agaggttcag
     3061 aaatcgaata cacttgaacc tgatgttgtt ggcacaactc aataaattct aaggctattt
     3121 tggcttgacg tgccaatcga tcataacgcc aaacatagat caccccctct tcttctgacg
     3181 cacaaaccca agctctaagc tgtcgaaaag ctggacgctc cgctttttcc cctgattctc
     3241 ctttgtctga aaatcgttgg ggaatttgtt catatttctc taaaacatac tctttcactg
     3301 catcatattg agcgtcatta ctaattttcg cttcttcata aacagacgtt cgatcataac
     3361 aagccacacg catgcttgat tcccctctct atgaatgaac taaataccat tttttcacga
     3421 ctcttctatt gaacgaacgt tgcacaaagt gattttctaa atatccttcg ttcttcaacg
     3481 cttgaagagt acccccgata gatccagcag atacccctct tatttcgaaa tccaagtaaa
     3541 ttggttttgt aaaaaaggct tgatttgaaa aatgttcttg aatatacaga agaacctctg
     3601 cttttagtgt cattttattt ttagtcatta gaattttcct ttctttcatg tttgattgaa
     3661 tgaagtaaat gaagcataga tcaagcttca tttacttccg tttgggtttg ctcgctatca
     3721 tacagataag taatcagttg ttttgctaaa tcaataagaa atatttccat aacatggtgc
     3781 ctcactttca tactttggtt tattctaaat tacaatcgaa aatacgattt gagtgaagaa
     3841 ggtaatagaa tctcgtatgt tggaagaact tgacgattca tgtgaatacg tttcgttctt
     3901 ctcccttttt ctgcacacat attacccgaa tcaagcaatt gcctaaaaag ataggttaga
     3961 tcctttactt gaaattctcg ccttaacaag tcctcaaacg ggataggaaa aatatttacc
     4021 ttaatatccc catttttttg aattgatact tttccccaaa tcgtactggg cgtatgtttg
     4081 ttgtcactgt ttgttaaaaa tttatggcta ttcatcacca acaatgtttt cactcggtca
     4141 aataccgttt gctcacttcc taaactagta tctacaatta actgttcgta cgaattaata
     4201 agcaccgtct gtatatgctc taatcgtaaa ggatagccca gtatatcttg aattaggagg
     4261 gctccactat aaatcaaagc tagattatta ccaatgcgtt gcttcaaaga atgttcaggt
     4321 aaaacagccc atagtttttt ttgagcttcg acaaaagttg cttcaatttt tgtaatccct
     4381 tgttcaaaga gtttcaaaag aaataacttt ataccccaac caaaagtatt agaagaaaga
     4441 cttttaactt tctctgcgtg attcgcatcc tttgtaaact tgatatcaga aaattcgata
     4501 tatcgaactt tcaaaccagt taatttctca ttgaaataat tttccaaacg ctcttcagaa
     4561 gaggtaaggg tcaaaacaga aaaagtctct tgtggcataa gctgacaatt agaatctaat
     4621 cttagccttt cttctcctga agctaattga tagatcaacg agctaaagtt attttgagtt
     4681 gctgctccta actcatctag tattagtgga acacctccct gattgtttga tgctagcttt
     4741 ataatggctt tattggtagc attcatattt ctaagaaccg tagttggatt tccaaataaa
     4801 gaggcaataa agtgtaacgt agtggtttta cccgttgtgc tcttgccctg taggttaatt
     4861 agtaaactca aaaaagtatt agccttcgta acgtttaaat agcctaacac aatagaagca
     4921 ataccaatcg accaagcaag ttctaagttc gttcctaaaa catacttatg aaccaatgcg
     4981 tcaaattccg cttctgtacc attagttgtt aattggtagc atgagttttt agttaataca
     5041 gaaggaattt cacgtccaaa agaatcttgt aactgaaaaa tcattgcttc attttctttt
     5101 ttccagccaa gagattcatg ttcaaaaaag attggagcat tctcccgttg aatctgtaaa
     5161 aagtcaacga cttcagcttc tcttctagct ttaatgatta agccatgttt tgctaattgt
     5221 ttaaaattat tagtttgtaa acatccacct tctatttcta tttcatccca acaatgatcg
     5281 acataatagg caagaacaag tgtaatgact ttttgttcga tatttttgcg tatttgctta
     5341 ataaacatcg gacaagcaac tttttcttct tgaccttctt tcaccaaaaa tactccttct
     5401 tgattcagtt taaactggtc aaaataatat tcttgattgg attctaatga ttctacagaa
     5461 ggatttacag gttttggcaa cggatatttt tttacaaagt cttgaatatc tttattttta
     5521 tccgaagcat gcagacaatg aaatcgaatc ttattatcag ccgtaacaga caaacaagaa
     5581 ccattagaat tattcgtgtg ttttcttaat ggacattcgt caaatataag taaggtaact
     5641 cctttatgat cgccctcttt tatggtataa gttaattcag gatagtgagc taaccattgt
     5701 ttatagtctg cttgaataaa aggtaacact tctctaaaag atggatggct ttttactcta
     5761 gaggttgaca tcatggaatt ttcttcaact atctgttcaa gaattttaaa acaattactt
     5821 ctatctatgt tatcgggaat agaaaggaaa ctagataaac gatgcggacg ctcttttgtg
     5881 gactttcctt tgaccgctgg tgtaccaact aatttgcata atcttgaagg attataaaca
     5941 gaagtatcaa tatcaacttc atcttctact ccaaatcttc ggtgaagtaa ttgaagaaaa
     6001 ttctgaatag taagaatcgt ctcgtgatta tttggttgtt catttatggg aagaaaaata
     6061 tggtagccat tcccactatc aacttttaca aatgattcta ttctcttttc tttcaaataa
     6121 cgttctactt gttccataac attttctgcc tttttctttt cgctatctgt tgcacttccc
     6181 tctttccttt tagggtcaat atcaataaat acaaaccgaa ttttttcaat atcattgtct
     6241 ctcacagaat gctttttttg tgtaatcacc tcttgtttaa taggattata agttatgttt
     6301 actgtacctt cagacaattt ctgtttttct aaatagttaa taattgcttg agaatctgca
     6361 gtagaaaaaa tatgagaaac ggttttcttg tttaatgtag aaaaagttct caaattaaca
     6421 taagaaatcc ccagtgtatt taacagttgt aaatgcttct gaattgaagt gtgattcatt
     6481 ttcattaatt accactcact ttctaaaaaa tttttatttg tatgactaca ttacctccaa
     6541 cctctcataa tttcatgatc ttcagttcta caaaaatata tcccactcaa aatcttgaca
     6601 aaagaaaaaa gttgtgattt tggaaatcac aacttttttc ttttacgata aatcaatcct
     6661 tattaaatta tttaggaaaa tattgttcaa ttatttcttt atatattttc tcatagtatg
     6721 tttctgtcca tcctaatgtt cctataaaat caagtcctct acattttaaa ggttcactta
     6781 aactattaat atagttaata atgttgtcta tccttttctc tttatcttca ccttcaatct
     6841 ggggagcata tagtccaatt tcctcccaat aatatgaatc tctttcaatt cccatgatgg
     6901 tagctttggc taatttcaaa tcaaataacg attttagaat tcttagtgtc tcaataaagt
     6961 cttctctctt caactcttta atccattgtg aattttctgg attatctaaa atttcaagtt
     7021 catcttcatt ataagtatat ttcaaatttg acttgcttaa ctcatagata aatgaagttt
     7081 cttgactcat aattctttta agtacttgac tataagtttc gtttgacttt gcccatttcg
     7141 ctaattctac cactgtattc actaataaat cgaaataatg atgttctcgt tcaatagtct
     7201 cagcattttg aaaatgaaaa tcacctttat aaactccatt cttttccact gttttattaa
     7261 tattttttaa aaaccagaat gagtttgcaa atttagtcgt actttttcga ttaactaagt
     7321 taataactct tccatgtaat aataagacaa aaatacataa atttttagct tccttattat
     7381 tagatactaa ttgatcaatc atagggagcc attttttgat tttcactaat tgattgctca
     7441 taaatctttc actggacttt tgttcaatct taaattgcaa aacttctaac tcataattcc
     7501 catcttctga tttatttttg ggaacaacta tcaaactttt ataaagttta tccacatcaa
     7561 aatttatctt ttttgcataa tttgtagcca ttaatacatc tccttattca taataaattt
     7621 catatgacgc tcctctcaaa atttaccggt agactagaaa aaagaataaa agatacgtca
     7681 attcacactt tattcttaaa aaaaaactgg tctctctacc ggtaatatcg attgagtccg
     7741 cctttatcga ctttatcctt tttttatatt aaattttttc accggtagac agggagagaa
     7801 agaataaact aaaaagccct taattcttaa tgttttcacc tccttaccgt tcctaccgct
     7861 aaaatttaat ataactaaat tctataacat tagaagaaat tttaaatacc tttgataaaa
     7921 aatattgatg ttttttatct cattgtttac aaactagata gcttatgtat tcttattaaa
     7981 aataatagat atttcgaaca tcatcactct catctactta atttcgcatt tttactttat
     8041 aaaaaaactg attctaagaa aattaaataa tttttctcaa aatcagtttt tctttgataa
     8101 ataatatttt caattattta atcatttgaa aattgttagt cattccaata cgttgagaaa
     8161 ttcttccatc tgtataaaca cggaattgtt ttccaccagc cattgttgta tttccattat
     8221 catccgttac aatattaatt gaatagtagc ttccattttc atcttgcatt agatctttca
     8281 tatattcatt tgtgctaatt aaattagtat ttgatgcatt tttatcagga gaatcataca
     8341 accataaagc tttattattt agtacagttt ctttagcagc ttcccaactt gaaattgctg
     8401 agctacttgt agattgtgtc gttgtagttg ttgcttgttg agtagattct tctactactg
     8461 gagtttgagg agtttgtcca ttcacaatat caatgaaacc ttgttttaac tgttggttct
     8521 gagtttcatt gaagtacatg tagtttttat cattgccttg attttgttgc gttaaataaa
     8581 ctttaggttg tccattttga aagccaaaga aatatacatg ctgccctaaa tattctccag
     8641 tttctacatc agaataaact gctactaaat tgaaatctgc atgaccagtt ccatcttctg
     8701 accattgtac ggtaactggt gcttcattga ttgccatttt ccaatttcca tcaataactg
     8761 cacttggtaa aggcgtacca tacagactta cattgttctg aggattgtag cttttatatt
     8821 cttgacctaa tgtctttccc cattgtggga caaaaattgc tagttgagta gatttctcac
     8881 tatcccacaa agtattttta acctcttcag aagaagattc aatgattgaa ctttcacttg
     8941 tacttgcttc cacagattgt ttcatttctg ttgtagtttg ttcagttgat tgcgttttac
     9001 tttttttagc ctcaattgtt gtatcagttt ctacagtctc tttagtcctc gtattctcag
     9061 atgtacaacc agtgaataaa atccccattc caattagtaa taacaaccat tttttcattt
     9121 tatttccccc tttcaatttc tccctcgcgt acttatttta tcagtcaatt aatctcaatg
     9181 gtgttcattt agttaatcct ttcccaagaa tgtcgatagc cagcatgaca acgatctctt
     9241 ccttgagggc tttcagatgg attaaaacta gtagaaattt cccttccaca tcctctacag
     9301 cgataagttc tcattatttt tgggctatta gtattatttt tcttagttag agctatgagt
     9361 ccaactatga ttaccccaac tgtcttaatt acttttcctt tatctatatt cggatgcgca
     9421 agcatatatt tatataacca atatccaccg taaattaaac cacctaatac aatcagctta
     9481 gccatgtact cgtctccttt ctttttgttt aattactttt tgaatccatc ttgcagagaa
     9541 accataaact tctgtcaatt gacggatatc tacagttttt tcctgttcta tcatgttttc
     9601 gattcttttt ctaactaact cacgatcgta cattttcatt ggaaaagaaa tctgttgccc
     9661 tcgaaaagca agataaaatt gatagagcaa ttctgaatcc agttccattt cctcaaaaaa
     9721 gtcattcata cttttataag agttactcaa acaagtttca tcaatctcca tctacacacc
     9781 ccctgttatt ttttacataa tgaaaatagc atattactgg gtacattgat aattcatata
     9841 acatgtatca tcacatgtat ttttaaagtc aaaaagcatg taaaactact tataagtaac
     9901 ataagaaaat aaaatgttat ttttcttgaa ataataattg actatctgcc aattgccatt
     9961 cattacgaac atgaatactt agtgggtggg ataacttacc ttctgcattt atcaatactt
    10021 tggtcatctt tttttgtttc gcactcaatt gatcgtaaat ggtcttcgct tgttcataag
    10081 ccacttgact cgtcgctgta atcgaaccac aagcaaaaat aatggtgttg attttctttt
    10141 ctgtgaaaac agttgttaat atttccaaag attgttcatc atttccacga ggaaataccc
    10201 gaggactttt cgcttttgta tacgaaaaaa gattcacagc aataaaacca gtaaatccta
    10261 attgtcggat attcttttcg attaataaca tactcaaatc attcgtaaat ggttctgtat
    10321 ctgttggacg aatagtaatg actaccgcta atttatcttc cgcttctttt cctttgatcg
    10381 cccattgtcg tttgaaaaca tagcgatggg tatgatgagg atctcgccaa acttctgttt
    10441 gtgtggtcac taattctttt tcaatcatct ctttttctcc tatgctaatt tttgttcaag
    10501 caattcaatc accattgccg ttataagtgt caatactagt tgaaaatatt tcatattctt
    10561 tctccttttt tcttcagaat ttgatgaatc gctgtaagga tatcttctaa acatttcgca
    10621 atcattctca aataatcgat cttattcatt cgttcctcca atctgactcc aaaccaatcg
    10681 tccttctacc agtaacccac gaacaaattc tcgctggctg gtaatatctg taatgaaata
    10741 aactacatag tcttttccat cttctggtgc gcaataaata taaagatgtg tcatcactaa
    10801 acgtggtaga gtaacatctt caggaagcat acccatttca atttcctctt tttccatcgt
    10861 aacggattca tagccccact cttcaggagt agtgactagc tgtgcacact tttccagtac
    10921 ttcgatagcc gtttctaaca agtcctttcg ttcctttctt ttagatatca gtattcttct
    10981 ggtaataaca tcgtttgata ctcacccgaa tcaatgaccc atattttttg agacactgat
    11041 ttcaaacgaa gcttaggtga aatcagatac tcttttcttg cttctggttc ttcttgacga
    11101 tgaaccaact tttgatttcc tttttccgta gaaaattcaa agatttgaag atagtctaaa
    11161 tgaaattgct tttgaacgtt ttgatcgatt agattccaac ataaagcttg taattccacc
    11221 ggcaaactgt ccattactcc ctttgtgaca taacgttgtc ctttattttg aaacatttct
    11281 tttccctcct ttgccataaa aatagcccaa tcttctatta aagaagattg ggcttcatgg
    11341 atataatata tatttgatat tcaatttgat tcattttata ctatctgatt tgtcaaatta
    11401 agctagacaa tatgtcttta tctatgcagc tcaaaaatac ttccaatggt gttcgataat
    11461 ttaatgattt tcttggaata tggtttcttt tcgaggcaac ggattgaata aaggtttcat
    11521 ccgtgtccct aaagtccatt gttttaggta agccatctcg tctaagcaag ccgtttgagt
    11581 gctcatttag ccctctttgc gaaggtgttc ctggatctgc gaaaaagatg gcaatatcgt
    11641 tcgagttact tatagatcgc cagttggaaa attcttttcc acaatcaaat gtcatggatt
    11701 taaacaagtg tttaggcatg gatttgaacc aatcatttaa cctgttttcg atatcttcgg
    11761 ctcgtctgcc tgtcggtttc aaagtaataa taaccttgga taaacactca accaatgtaa
    11821 taacagcgct cttgtgttta actcccacaa tggtgtcgac ttcgagatga ccaaattctt
    11881 tagtcaggtg tggatagaga ctgttccgtt catgaatcgt tctgcgaaag gcctgttttc
    11941 cacgcttttc ttgatgatta ttcggtttac gtttcccctt cataggtaac gactgtgtgt
    12001 caaaaaggcc ttttttaaac atccgataga gtgtacgaac agaacaatca attggaaact
    12061 cgccacggcc gataatcaca tcgggtgacc atccttgagc taccttttct tgaacatatg
    12121 ttgattgttc atcagataaa acggttgaac gtcgtccaca acgagattta ttttgtttgt
    12181 attgacggta ataatctagc actttttttc cttgtttgag aaacgaataa acattataaa
    12241 tggtttgtct agagcgattt aaactttgtg cgacaagagc gacaggtttc tcgatattga
    12301 aataagactc tatgattact agttcgtctg ttgtaagatg ggtgtaagtc atttgtactc
    12361 actccttatt aattctttgg tcggaactat tttgagtgta gcacaaatga tctttttagt
    12421 tgtctagctt aattttacaa tcgacgtttt aataaattgt gatataatga agtataactt
    12481 tttagggagt gtactatgaa ttttcaagat aatagaccat tatatttaca agtagaagct
    12541 tctatacgta acgatattcg tgaaaaaaaa tatcttccag gagagcaatt acctacagaa
    12601 gaagaattat gtaaaattta tggagtaagt aagattacca ttcgaaaagc ttttaaactt
    12661 ttagcagaaa gtggaacagt tgaacgatta agggggaaag ggacctttgt aactcaaaaa
    12721 aaagaaagtt taaaattagc tggagttcat ggatttagcg attctctttc tacccgagga
    12781 cataaaatta gatatactgt tttacattct aaaattataa aggctgatga gtttttagct
    12841 aataaattac aaatcaatgt aactgataga gtttacaata ttaaacgttt aatgtgggaa
    12901 gacggtgccc cgatgggaat tgataatttt ttcgcatcag aagaaaaatt tccaacttta
    12961 tttgaaaaag gtagccaata tacttcatta tataaacttc tagaagatga ctatgatgtc
    13021 aaagctacga gctctattat ggaaataaac ggtttatctg caacacctga gctttccgac
    13081 ttattgcaat gtatagtagg agatccgcta tttattttag aaaaggttgg caaagataaa
    13141 aataatcaac catttcatta ttctcaatcc acagttcgtt gtgatagaat tagttatgtt
    13201 attgaagtca cagaaaatat cactttagat acggtgcata aaaatgattc agatcctaaa
    13261 gtaatagatt gatattaaaa gaaatcatac ccaaagcaat caattttgat tagatttggg
    13321 catgattttt atttttacta atattctcta aatacatcaa aaaacttatt tatattccaa
    13381 atttcacctg tacttcttgt cgcagatatt taatatcgta aataacatca tctgctagac
    13441 ttacatcttg agaaaaacgt ttatctttaa ttgcaagaac cttcatattt gctcttttac
    13501 cagcttttat accattataa gaatcttcta tcaccagaca ttcttcaact ggaattctga
    13561 gctgttctga agctttaagg aatatagtag gattaggttt actttctggc aaatctcttc
    13621 cgctaattaa acaatcaaaa tatgactcta ttttacaagt ggtaacaact tcatttatag
    13681 tatccattgg actggaagaa gcaattgcca tttttatttt tttctttttt aaccattcaa
    13741 gctgttcaat tagtatatta ggaaaaatat ccaagtaatc aatcttttgc gatttccaat
    13801 atttagtaaa ttttcccttc aattctaaac tgttacatcc ctcattgtac ttttctatat
    13861 catcaaaaat acttgctgta gttccaatct ttttttgata agaatacaaa ggatagctta
    13921 aattttcttg ttttaaaaaa ttaaataccc acttttgata cataatctcg ctatcaatta
    13981 aaactccgtc catatcaaaa attagagcct taatcattat aagataccta ttatacttaa
    14041 aacaaaccct gccaaaatac aacctaataa gagcatattt gtactaacat ttttcttaat
    14101 caatttatat attcccaaag tacatacaag tggtaataat ccaggaataa tggaatcaag
    14161 taagtcttgt aaaacaagtt ctgttccccc tgtagtgaat tttaaagggg tgacaattgg
    14221 caccatgcta gccaccattc cacctactac agccaaacca atgatagaag caaattgcat
    14281 tactcgatcc ataagacctt cttgatacaa tttagtaagg tatttattgc cagactcata
    14341 tcctaattta ccgccgtaaa aaacagctaa aatagaaggt atagcaaaaa ggccaacagc
    14401 taaaatcggt cctaaaatac taccttgctt agcaaaagac aatccaatcc caaatgtaat
    14461 aattcttaaa gtaccttgga aaaatgaatc accaattcca gcaaaaggac ccattaaact
    14521 ggttttaatc atggagatag attcagtatt aaagtttcct tcttctgctt gagcattttg
    14581 ttcttccata gaagcacaaa gtcccataat aaatgaagtc atttgaggcg tacagttaaa
    14641 aaatgccata tgacgagtat aagcttctct ttttttctct tcatcatttt tataaacttt
    14701 atttaaaatt ggcgccatcc catacataaa tcccatatgc atttgtcttt cataattcca
    14761 cgaacacatg attgtccatg aacgaagaca catcttatta agatcttttt ttgtgattaa
    14821 acgttttttt tctgtactat tttccattaa ccatgcgcct cttctctttt tccatattta
    14881 acttgatcca ttacaaaggc aatcagagct gcaaaaattg tcacgccaat tagtgaaaga
    14941 cctgaatagg caacaataaa gaatccaaaa ataaagtaag gatataattt tttactcaac
    15001 attgtgctta atagcattgc aaaccctaat gcggaaagca tatttccaga gacagtcaag
    15061 gcgtctgtaa tcatttctgg aattttatct actaaatctg ccacaacgtc agcaccaaaa
    15121 tatactgtta aaaagattgg aataaagtaa acaaaagcat taaaaataaa tgaccaataa
    15181 atatgaataa tttgagcttt tctaaattta ttattttcaa tggctttatc tgcagcatgt
    15241 ccaaattgag caattatcat gcgcatgata atactcataa attcaccagc tacagccact
    15301 gggatagcta ttgttaaggc tgtttctgta ctactattag ttaaaattgc aaaagcagtg
    15361 gcaataatac taccaagttg catattagga ggtccagccg ccccaattga gacgaatccc
    15421 atagaaatta attctaaaga tgccccaaga acaatccctg tatgcaagtc acctagcaca
    15481 agtccggcaa gtgtactcaa aattagtggt ctaccaatat tttgtcgacc aattaacctg
    15541 gagtcaataa cagctaccac accaactaaa cctaataaaa tggcttttaa taacatttat
    15601 tttcccccct tacttactaa aatctacatt ttcaatactt gaagatggga cttgttgcac
    15661 aaatattttc acttcttgat caagaatttt ttttgtggct tctaattctt caggagttaa
    15721 aaagactgcc tttccatatt gtttggactc gcttttctta gacacaccgc caaaattaat
    15781 ttctttaatt tctggtaaat tttgaactaa ttcaaaagct tccttaggtc ctttaattaa
    15841 aatcatgtaa ttatcatttg gggatgtttc cataatccct ttaacatcac taattttaac
    15901 aatgtctaat ttgcaatcag caggtttagc aattgtcaaa gccatcacag acattggatc
    15961 tcctggaact ttgttatcag ccacaataat atggttaata tcatactgtt tagtccaaga
    16021 aaatattact tgtccatgaa gtaatctgtg gtctacacgc actaatttaa tcattctatt
    16081 tcctccttca atttttaaaa tttatagaat agaaattgcc accttaactg acatctttta
    16141 aaagcgaatt aatatgaacc atactttttt gagagttatt cagtaattcc ttaaaatcta
    16201 tttcttctat agatttacca gctgactggg ccattaataa ctccagtaaa agaggtaaat
    16261 taatcccagc aataatttga attctattgt tgttcaactg tttaatagaa acattattag
    16321 gtgttcctcc aattaaatcc gttaaaataa ttagaggttg caaatgatgc tgactaatta
    16381 tttctttcat actctcctca aaatcatctg gactagtatt ttctttaaat ggaacagcac
    16441 taatacctgt ggcgtcattc attaaaatta attttgttgc ctttaaataa ccctcacaaa
    16501 gggtagaatg tgttgtaatc acaatgtgaa ataattgatt tgccatcttt tgcaacctcc
    16561 tattaattaa tatttcattt gccacatata gcgtctaaca tctaaggaat ggcctctttc
    16621 aaaagcaatc gcttctacca tttggcgaat aacaacgcca ctaagtacag ctgcaatata
    16681 ttctttcgct acactatcta tagattctaa gtcaaaatca gcttggtcta aaaccattaa
    16741 tttattactg aattttttag tgaagtcttc tacccgttga tctaaaaatc ttgtatctcc
    16801 aatacttttc actaagatga atggtacatc ataatcagtg atttcaaaag gaccatggaa
    16861 aaattcccca gaatgaatag cattagaatt aatccattgc atttccatga accagcaagc
    16921 tgctgtggag taaatttctc cgtaattgat accgctacca attgtgtaaa taattttatc
    16981 tcgtttttgg tttttacccc attctttagc agttatttca aatttgctct tttcagcaat
    17041 aacaagatca tttaacttat caaattcatt caagcaagtt tcccattttt tagagccatc
    17101 taaaacaagt aaaatattaa ataataaacc ataaagaatt cctttattta aatcactagc
    17161 atcaacatct ttaccccaat catagtgaac aggatattca gctgcttccc atagaggaga
    17221 tccttcttca ttagataaac aaatagtcat tgctccaaat tctcttgctt tttttgttgc
    17281 ttctacagtt tctggtgttg tgccagaatg ggaacaagtg ataactaaag aattttcaga
    17341 taatcctttt ggtatagcat agttaaattc gttagcagta taaacatgac aaggtacagt
    17401 taattcttta tcaaatagat attgaccagc cattaaaacg gcttgtgaac ccccacatgc
    17461 tgtaaaatat acatgatcaa ttgtcttttc ctttaacgct gccattgctg tttcaatctg
    17521 ttctttgtgt agttgtctca tttctttatc cctcactcta ataagtatta taaagtcata
    17581 taactttaca atacctatta tctatgaaat tgctttattt tgcaatacta tttttcaaaa
    17641 aaattttttt ataacaattt tatataataa aat
//
LOCUS       DACDYI010000067        17244 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.067, whole genome shotgun sequence.
ACCESSION   DACDYI010000067 DACDYI010000000
VERSION     DACDYI010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.067"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            492..1625
                     /locus_tag="IWM52_002199"
     CDS             492..1625
                     /locus_tag="IWM52_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP9974429.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            1656..1976
                     /locus_tag="IWM52_002200"
     CDS             1656..1976
                     /locus_tag="IWM52_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974430.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(2005..2469)
                     /locus_tag="IWM52_002201"
     CDS             complement(2005..2469)
                     /locus_tag="IWM52_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9974431.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(2601..3854)
                     /locus_tag="IWM52_002202"
     CDS             complement(2601..3854)
                     /locus_tag="IWM52_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9974432.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLKILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(3883..4200)
                     /locus_tag="IWM52_002203"
     CDS             complement(3883..4200)
                     /locus_tag="IWM52_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9974433.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(4226..4546)
                     /locus_tag="IWM52_002204"
     CDS             complement(4226..4546)
                     /locus_tag="IWM52_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9974434.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(5010..6425)
                     /locus_tag="IWM52_002205"
     CDS             complement(5010..6425)
                     /locus_tag="IWM52_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9974435.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(6442..7758)
                     /locus_tag="IWM52_002206"
     CDS             complement(6442..7758)
                     /locus_tag="IWM52_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9974436.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(7908..8606)
                     /locus_tag="IWM52_002207"
     CDS             complement(7908..8606)
                     /locus_tag="IWM52_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAP9974437.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(8895..9884)
                     /locus_tag="IWM52_002208"
     CDS             complement(8895..9884)
                     /locus_tag="IWM52_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAP9974438.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(9964..10797)
                     /gene="msrA"
                     /locus_tag="IWM52_002209"
     CDS             complement(9964..10797)
                     /gene="msrA"
                     /locus_tag="IWM52_002209"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP9974439.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(10972..12240)
                     /locus_tag="IWM52_002210"
                     /pseudo
     CDS             complement(10972..12240)
                     /locus_tag="IWM52_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12418..12714
                     /locus_tag="IWM52_002211"
     CDS             12418..12714
                     /locus_tag="IWM52_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974440.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            12721..14673
                     /locus_tag="IWM52_002212"
     CDS             12721..14673
                     /locus_tag="IWM52_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP9974441.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(14745..15242)
                     /gene="dfrG"
                     /locus_tag="IWM52_002213"
     CDS             complement(14745..15242)
                     /gene="dfrG"
                     /locus_tag="IWM52_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP9974442.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(15878..17212)
                     /locus_tag="IWM52_002214"
     CDS             complement(15878..17212)
                     /locus_tag="IWM52_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974443.1"
                     /translation="MDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAY
                     GLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVV
                     NLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITT
                     YTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQF
                     PWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQS
                     KHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAAD
                     NNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEIT
                     TKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
BASE COUNT         5513 a         3323 c         2852 g         5556 t
ORIGIN      
        1 ataagagcag ccaagctaaa acagcttctt atgtttttga cttggttaga cttattaccg
       61 cagatgcaag cttttgaaca ccgtttacca agaggttatg aaaaaggcgt ttagtttcga
      121 gtatgaagaa aaattttgtg aaaatggttc ctcacgtttt tgcataaatt tgacttaata
      181 ccgagaaact gatcccttct gtcataacct ctttttattt atgtctgttt ctttttgaaa
      241 acaactattt tttcaacgtt tactctcttg ccaatccaac atcatctaac ggatacaact
      301 atttcaatga ttaccattac actaaaaaat cggaaacaca cctctccgaa cacaaataca
      361 taagactata gaatgtttca tttaagaaaa aaagcgtttc attatcaaaa aggtaagatc
      421 tcctttaaaa tgaaattatc tgtagtacta ataatgcaga caattttatg aatctgaaag
      481 gagcatctcc aatgaaagca gtcacttggc aaggaaaaca gaaaatggaa atcagaaacg
      541 tagatgatcc aaaaatatta gaaccaacag atgcaatcat ccgtatcaca gcaacagcca
      601 tttgcggctc cgatctgcat ctttaccatc atggtgaagc agttttggaa aaagggtatg
      661 ttgtaggaca cgaaccaatg ggaatcgttg aagaagtggg atctcaagtt caaacattga
      721 aaaaagggca acgtgtggtt atccctttta atattagttg tggtcattgt catttctgcc
      781 tgaatcacat ggaaagccaa tgtgatgaat caaatccaga tcagttatat ggcgggcttt
      841 tcggatttgg caagttgaac ggtaatcatt ggggtggtca agcggaatat ctaagagttc
      901 cttatgcaga ctctacttca tttgttgtac cagataatga tcttcctgat gaaaaagttt
      961 tatttctatc agatattttg cccacagctt attggagcgt tgaaaacgca ggagtaaaaa
     1021 aaggcgatac agttgtgatt ctaggttctg gtccagtcgg tctattcgct caaaaattcg
     1081 ctgttatggc tggagcagag cgcgtaatcg cagtagatcc agtacagcac cgtctagata
     1141 aagcggctaa ttacaatggg gttgaaactt atttcttaga agacacggca caagctggaa
     1201 cagaattgta tgaactggct aaaggtggcg cagatgtcat tatcgattgt gtaggaatgg
     1261 acggattaga gcctgtcaaa gaaaaagcga aaaatttagt aagcttacaa agcggcacaa
     1321 tctctccatt acagatggca tcgcaagctg tcaaaaaatt tggaacagtc caagtaactg
     1381 gtgtttatat gactccagct tcttcttacc cgctgcaaga attttttatg cgggatatcg
     1441 ctgtcaagca tggacaggca ccaattatcc atctaatgcc aaaaatttac gcaatgattg
     1501 cagaaggact attcgatcct acacaaatca ttactcattc gctgccacta tcagaagcag
     1561 cacaagcata tgagatcttt gacaaaaaag aagataaaaa tataaaagtt gttttgaaac
     1621 cttgaattca gtaaaaaata caggtgattt tctttatgcc caaaatcaaa cgaaataccc
     1681 atgagatcga aataggaaaa gaagaagata tcgaaatacg aggacgtcgc ttccgcttct
     1741 atttccaaaa tcgttacaca ttgatctcac tggctgtcga tttattaacc gggattttct
     1801 atatttttgg aagccttgct tccttaacta aaatccccga tcgatatggc acctattttt
     1861 atttggctgg tgctattttc ttgacgattc gtcctattct cagaatcgta aggaacattt
     1921 ttatttacga cgaaaaaaaa gcaatgaaga gaacaagcaa aaaaaaagag gcatgagaat
     1981 ttttcaaatc tcatgcctct tttttcaccc tatcgtatca ctttgataca gacgagcggt
     2041 cgtcccactt tcttccttga ccattgtcaa aaatgtccac tcgtatcgtt cataaaacgt
     2101 ggtatgatct gtaattaagt aaagtttttc gtacccaagt tgatgcattt cttttctgat
     2161 ttccgctaat atccgcttgg ctatcccctg cttacgcttt tccggttcaa caaacagggc
     2221 acaaagattt ggtgtcagat cagggcggtt atggaagtcg ttttcaatga cgcccgctcc
     2281 tccgacgatg gtttcttttt catctaagac cacataccat tgaggaatac tttccttatt
     2341 agcgattcca gtacgaatac tttcctcata cacaacagca ggaactcccc attttgatga
     2401 aaaccaattc gcgctttgtt ttactaattc tgggtgttct cttaatttga tccagttata
     2461 tgtaaccaat gtttaaacct ccgactaaaa taaaacgttt acaaaaatta tacaagtctt
     2521 tatgaaaatt agcaatgaca acaatagtga tgtaaaaaaa aaaacggaaa acaatcactt
     2581 gttctccgtt ttttgattaa tcaagcatct aaagcttctt ttcttctttc ttggatcgta
     2641 gctaccttga cgaacggtag atagatcaaa atagaaagaa ccaaattgac agcggctagt
     2701 acaccacctg caatactttt tgttgccaat acaccgccaa taattggtgg agttacccat
     2761 ggaggcataa cggtagctgc tggcactaaa cctgtagaag tcgcaaagta agcaacacta
     2821 actaagacca ttggtgtcaa aataaatgga atgaacatga tcgggttcaa cacgataggt
     2881 aacccaaaca tcgttggttc attaatattg aagacacctg gcgcaatcga caagtttgtt
     2941 acaaccatat atggtttgtt tttacgtcct actaaataaa tagctaacag taaacctaaa
     3001 gttgcacctg ttcctcctaa gttgacaaag ctgtcaaaga aaggtttgtt cacgataaac
     3061 ggtgcgctat gtccagctgc gattgcatta acgtttgctt caatggctgg cgcattgatt
     3121 gtttgcatca aaggatcgac catattcgct ccgtgtaaac caaagaacca taagaatggt
     3181 gtgataaatg caagcagtaa ggcagatgga taagagtttg caagtcccat aaatggttct
     3241 tggaccactg tatagaaaga acctacgata tcatcaacac cgaatgcagc aaaaattgca
     3301 gcaactaaag caaacgcaga gatagtgatc attgctggta gtaatgaagc aaatgctttt
     3361 gcaactgccg gtggcacacc atcaggcatt ttgatgatca atttttcatt tccgcttaaa
     3421 cgtacaaaga tttctgttga aataatccca acaataatag caataaacaa accgtttgag
     3481 ctcataccta atgctccgcc taaagcgaag aaagagccta aagaaatcac acctgcggaa
     3541 acaccgtctt ttcggtagcc gttagctaaa tgataagcaa gcaagaaagc aatcaagaca
     3601 gaaagaatgg caaatgtacc gttccaagca gcagccccga atcctttcca tgattcacca
     3661 ccaaaaattg agttcatcaa ttcttggaac ccgggaatcg gtaagttatt gatcattact
     3721 gctaatgctc ctaagatctt taacggcata ctcaccataa atgagtcccg aatcgcaact
     3781 aaatgccgtt gtgcaccaat ttttgatgct acaggtataa aatgtttctc cataaatgca
     3841 acaaatttat ccataaatct tccctctttt ctttactgta gattacgcgt tcaatcgacg
     3901 atacagatca atgatctctt ttgctaaatc tgtaaaagct atagatgtca tcagatgatc
     3961 ttgactgtgg accgtcaaaa gcgtgacttc aacgtgattt ccttgtgctt cttgtgtcag
     4021 catctctgtt tgagagtgat gtgcttggac taaagattcc tctgcatctt tgattttttc
     4081 atctgctaaa gtgaaatcac cttttttcgc agcagcaatc gcttccattg catcgctttt
     4141 tgcatttccg ccaaacatga tcagacccat gatggcttct agattttttt gatcttccat
     4201 ttattgtatc ctccgataat ttttattatt tgtttattag tttttctgct tggtcgagta
     4261 ctttttcacc attcatcatg ccatagtcag ccatattgat tacatctaaa ggaatgcctt
     4321 taggtgctag cttttcagca aattgatttt gcataaagcg tacttgcggt cctaaaagta
     4381 aaacgtctac atttttagat tctagttgat tatccgcatc tgaagcagat acagcaaaga
     4441 tgtctgcttc cattcctttt gcttctgctg ccttttgcat ttttgtgact aggagacttg
     4501 tactcattcc agctgaacat acaagcatga ttgttttctt ttccatttta aacacgttcc
     4561 tttctatact tacacttgta tataagcaag tagcgtgcca actttaaaag cgctttaaaa
     4621 agttgatatc atcacattta aggtctatac caagttgcat acacactaaa aaagaaagcg
     4681 cacactaaac acacaaatta atttttatta ccttttttct tttaacgtgc acaaaaaaag
     4741 ttgtgacaca aaacttgtca caactttttt caaataaacg gtattcaaaa gcttgcatct
     4801 gcggtaataa gtccaaccaa gttaaaaaca taagatgttg tttttacttt gctattctta
     4861 ttacttgatg caggacagct ttttcacaac ctctcaataa acggtgttca atcagctgac
     4921 cctgggtatt agctcaaaac gctggattca caacctcttt tatttcaaat aaccagtaaa
     4981 tacaatgaac atctacaaaa aacttctatt tatttataga tttcgtcagg caaatccaat
     5041 tctctcttat caatcaaagt ctgataccac gaagcagatt ttttcaatgt tctgcttcgt
     5101 tcatcgttca attcgattcg aactaatcca tatcgattct taaatgcatt cattggtgac
     5161 acacagtcag tgaatgccca taacatatag ccttcgcaat ttgcgccttc ttctgttgct
     5221 ttgatcaagt aatgcaaatg ctgtccgata aattcgatac ggtaatcatc ttgcacgatt
     5281 cctttctcat ccatatactg ttcttcatat tctcttccca taccgttttc tgttatcagc
     5341 caagggatat tatgataatt ttcttttaga tacatggcta tatcatacat gattttcgga
     5401 tagatttccc atcccctgga attattcatt ttcttcccag gtaaatcaaa gacttcataa
     5461 tatcgttcag gatggaaagg tgtctcttca ttccattgat aacgaggggc gcgaacgcgt
     5521 ttaggataat attgattgat tccaaggaaa tctactgtat gatgctgaat cgtttctaaa
     5581 tcctctgttg caggatcgaa ataaatcgat tgtttttgac acaactccag gaactcttct
     5641 gtgtagtatc cttttaccat cggatcaaaa aatactcgat tataaaacaa atcatacata
     5701 taagcagctt tttggtcttc tggagaattg gatcgagcat agaccatttc aggatttaaa
     5761 atacagccaa tccttccacc ataatttccg ctgcggtaaa tctctactgc tttagccgtt
     5821 gctaaaactt tatgataatt ccacatcatc cacttttttg tgttttgttc atgcggccag
     5881 cgaacagcat ccaaatagca acgcgtttgg ggaacgattg gttcgttgaa tgtaaaccag
     5941 ttttttaccc tatcaccgta acgatcaaag gcaatttgag catacttagc ataaaaatca
     6001 actacttttc ttgaagacca gccatcatat ttttccaaaa gaaaagctgg gacttcataa
     6061 tgttccaaac aaatcatcgg ttctacgtct gcttcgatca aagagtcgat catttcactt
     6121 atatgctgag catattcttc atctactata cagttctcat aatcagtaaa aaaacgagcc
     6181 caattgatag aagtccgata atgtgtcaga tggatggatt tcatcagatc cacatcctgc
     6241 tggtattgtt ccataaaatt agttgctacg gctgggccat acccattatg ccaaacaaaa
     6301 tgttcatttt tataccagct atctataaac gaatcctgtc cttctttttt tcctttccaa
     6361 ccttccgttt gccaagcaga agatgcagca ccaagaataa aattttttgg aatagtaatt
     6421 ttcatgattt cctcctgttt tttagatccc atcattttcc acacgatttg ctacttttac
     6481 aaatggcaga tagatcaaaa tagaaacaat aatacaaaca agttgcgtaa taactgcacc
     6541 gatatttcct gctgttgcca gataagcatt gacgatcggc ggcattggcc aagggaccat
     6601 tacaactgct ttcgccgcaa atcctaaagc ggttgcaaaa taaccaatcg ttccagtgat
     6661 aagaggcgtt aagataaacg gaatagctaa aattggattc agcatgatcg gcataccaaa
     6721 aataactggt tcattgatgt tgaagatccc aggagcaagg gataacttgg tgatttgttt
     6781 catatcttcc cgttttccaa ctaacaatac tgcaatcagt aaaccgatcg tgactccaga
     6841 cccgcccata ctcatataca tatcccaaaa tggcatagta ataatgttcg gtacttcttt
     6901 tccagcttca aaagcttccg tatttacagc aattgccgct aaaagcaacg gttcccgtat
     6961 cggcttcacc atttgatttc catggatacc aatcacccaa aagacttgcg caataaacat
     7021 caatagcaaa atacccggta acccttgtac gacaccttct aatgggcgtt gtacgacatt
     7081 gtaaatgata tcataaaggt acattcctgt aagttctttg atgataaatc ccgcagccgc
     7141 gatacccgat acagtcagaa tcgtcgggaa taaagcagaa aaactagcag aaacattcgt
     7201 tggcacggta tcaggcattc tcagctgtag tttcttctgt ttcctcaacc agcaataaag
     7261 ttcaatagaa aggatggaga ccaacattcc caaaaataac cctttcgtat ctgtatattg
     7321 tttagccaat acgttttcta ctataacagt ctgattttca ttcactaatt cataaacagt
     7381 tggattaaca gataaataag acatcactgc gatgatacca gggaatacac catccacttt
     7441 attcagcttg gctaattcga tccctatcag aaaaacagca tagatggtca agaaacttag
     7501 tgtgacatag ctgatggcag atgcgatagg ctttaattct tctaaaaaac gcaagaaact
     7561 tatctgtgct aagccatttt ccgagtcaaa gaccattgct gaaaacaatg tgccaaaagc
     7621 acccgtaata attaccggaa tcaatgcagc aaatgcgttt tttatcacca tgatatacct
     7681 taaattattg attctcgttg ccacgttccc taaaacattg gctaatttat ccataaaact
     7741 cattccagat cccctcattc ctgcttcttc tccttgacgt catctctttt tgtctattag
     7801 tcagcgctta catttcgatg aacagaaact atttactcct cttttttacc aaatataaaa
     7861 caaaaattca tctgatgtat ttatcataaa atacatcaga tgaactgtca attagtttct
     7921 ttttctccta ttattttctg taattctctc cgatttattg ccatatggac agtcattgca
     7981 tcaaacgcag caagtgcatt ccgcctttcg atggcctgaa cgatctctgt atgaatatcg
     8041 atcgtctcga ttttcagatg ctggatatca taatttttat taaacaaagc aattgattca
     8101 ttaatgattg ggataatatg cagcaaggct acgtttccgc tagcttctgc aatcatcgta
     8161 tgaaaatcga tgtctaattg aacatgttct tgtgtgcctt ctcgaaaaga agtttcaatc
     8221 ttttgcgtta attttttcat ttggacgatt tgattcttcg ttgcatattc cgctgccaaa
     8281 cctgccatct taggctccaa taaatatctc agttcaaata agtctcgtat catccgatcc
     8341 tgatcttcaa tcaaagaaaa accgagtggg tcattggcaa tccctcgctt atcactgaca
     8401 aatgtccccg ctccttgccg aacttcaagg atattcctgg aagctagagc ccgcacagct
     8461 tctcgcaccg tgcttcgccc cacctctaaa taatcggaca attcatattc attcggcagt
     8521 ttatctccta cttgcaactg catttccttt aaatatctaa ggatcttgtc agtagttact
     8581 tctactaggg ttttattttc ttccaaacca catacctcct tttcatctga tgtatttttt
     8641 cgtaattatt ataacacgtt tgaatcatct gatgattttt tgtatacaaa attgattaat
     8701 ccgttcatag acatacaaac gaagaagata tttaccataa aaaagactag gataaaattc
     8761 actcctagcc ttcgatcatt tttattttga acgacaaaaa atatgatgtt caataacaga
     8821 cgatcttcat tcgattcttg atgtcatctt ttgttttttt taatatatgg ataaaaagtt
     8881 tgattacatc gtttctattc tagaatttgg tcgattctca attcttcttc tggtgagaaa
     8941 tctaaattat ccatcattcg aacactttct tgtaactgac ttaaccgact tgctccgacc
     9001 agtacacttg taacagattt ttgtcgcaaa ttccatgcaa gggccatttg cgctaatgat
     9061 tgtcctcgtg atacggcgat tgtttgcagt gctttgattt tttccagagt tgatccaact
     9121 tgttcttctg aaaggaatgg tatttcctta cgatgtgctc tagaatcttc aggaatacca
     9181 tgtagataac gatttgtcag caacccttga gcaagcggac tgaacgtgat tgcccctagc
     9241 ccttcttctt ctaatactgg agatagatcg tcttctagta gtgcacgaga aaacatattg
     9301 tagcgcatct gatgaataat gaacggtgct tcctttcttt ttaaaatttc agtcattttt
     9361 tttgtatctt ctccattata gttggatata ccaatatata atgcttttcc ttgtcttaca
     9421 agttgcatca aagcttctgc tgtttcttcc aaaggtgtat cagggtctgg gcgatgatga
     9481 tagaagatgt caacataatc tagccccatt ctttggagac tttggtcaca acttgaaatg
     9541 atggatttac gagagcccca ttccccatat ggcccttccc acatatgata acctgctttg
     9601 ctagaaataa tcagttcatc acggtaggct tgaaaatctt ctctaaaaat acgcccaaag
     9661 tttttctctg cagctccagc agggcctcca taattattcg caaggtcgaa atgggtaatt
     9721 cccatgtcga atgcgcctct taaaatctct ctttgattat ggataggatc ataatcacca
     9781 aaattttgcc ataatccgag agataaaatc ggtaatttca aacctgaatg accggattta
     9841 cgatattcca ttttttcata acgtgcacta tctgcaatat acaatttttc tcctcctcat
     9901 caaatctttt taaaacgctt tcttttttct atttttagtt tactacattt taaagaaaag
     9961 gagttaatca aaatgatttt ttcgtatatt tttttgccat ttgtttttta ctcgttgata
    10021 gatcagaaat tgattacgtg cttgctggat ttttttataa cgtttcggtt gttttttgta
    10081 aaattgctga tgatagtttt ctgctggcca aaacgtaccg gctggttcga tttttgtgac
    10141 gattggttgg tggaaagttc ctgaactgtc tagtttctgt tttgattgtt ccgctaattc
    10201 tttttgtcgt tcgacttgat aaaaaatcac tggacgatat tgcttccctc tatcttgaaa
    10261 ttgtccaaat gtatctgtgg gatcagtaat ttgccaatag attgtcagca actcgctata
    10321 acttatttct cttgtgtcat aaatgatctc cacagcttcc acatgacctg tgtaaccacc
    10381 acttacttga tcataatttg gtttttcaac atgtccgccc gtgtatcctg acaaaacaga
    10441 taggatccct tttttcgatt caaacggctc gaccatacac cagaaacatc cccctgcaaa
    10501 tactgcacgt tcttgatagt cagcatcttg catggcatgc ttggaaaagt catatttttg
    10561 ctccttatca aatccttccg taatcaataa ataaaagtcc attacatcag gcgtcagatt
    10621 cattcggata gctaatggac gtagtgttgc ttctagtttt gataactgtt ctttaacgga
    10681 tatattttct cttatgtcat tctttgtctg aatcaatact tttcgttccc aatctcgtgt
    10741 attcggattc aaaatcagat tataaagtgt ttgaatgaat atttgttcct catccataga
    10801 ttctcctcct ttccgaagaa gacaagctag tgactttagc tgtaatttat cataaagtga
    10861 ggaaataagc gattacttca tccccctaca gatttttcgt ataaaaaaga gagaatcctt
    10921 atcgacagat tctccttttt aatcaattta ttttataaat gaaaagttaa tttacctttt
    10981 atggaaaact attttaaaaa ataaacataa atatgcagtt aatgtaacaa gataagaaat
    11041 aatagtcgtt actgtataac ttgtgcttac tttaatatca gcaactcctt tgactccgct
    11101 tatttgtacc aatccattag cattcacctg ctcctttaca ggttgatgat ttatttctac
    11161 attcgttcct aaataagcat atacaggaag tgtgattact tggctcttct cattagttac
    11221 tctatatatg atagaactac cagttgtttt ataaggaagg tttacccatt gactgccaat
    11281 ctttgcttcg tgttttatta tatgctcctt attctccaat gaagcgatag gagaataatc
    11341 atataaaaaa taatccattc ctttagtggt atacgcctct actgtttctt tgtttacaac
    11401 aatatgtccc ggctgagaaa ttattttttg attgaaattt tttttactag aaacagttaa
    11461 agtgattaat aacaaaacag tcataaataa ataaaatagt tctcttgtct tattgtatct
    11521 tctatttttg aaaaatatca tcgttaatat cagagaagag aatattattt gcaagcttaa
    11581 gatccgccaa ggaaattgta acaatttggc tggcgtattt tgcagaagat accatggcac
    11641 caaactagtt gtagctagcc aagtcaaaca cgtaccataa taaatctttt tttctatgtt
    11701 tgttaatttt ttaaataaaa tcaggggaat acataaagat aacagaccaa ctagtcccat
    11761 tgagtaagta gttaagtcgt tgttcatagc ttcagtcaga ctatcgaaca cattagatgc
    11821 atgagcatat aaatttgtca cgctaggttt gttgattttt tggtagagca tttgttcaaa
    11881 catcggatac caaaaagcac tcgtcagaac caatgtcatt cctgccgcac taaataattt
    11941 tttaaatata gcaataaaat ctgcattctt gaaaccttta gaaaccaaac gaataaaaat
    12001 aaaaatgata attaatgctg tactcattaa tgttgatagt acatggctgt acactagcaa
    12061 actcatactc aatgctagct ttacagacgg atacttttct tcttttccag agatgatttg
    12121 ataagcataa aataatacag ggataacaag tgttattgcg atagcttcgg caattgcgga
    12181 acgatagtag atgtctactg tccgataagc agagaaaata tagagacaac taaataaaaa
    12241 taagtagttc agttttggag tacaaaaagt gaaaatgtaa acctaaatga gtatcaaatt
    12301 gcaactgaat ttgatactcg ttttttattg ttaaatcaac gtttgttgtt aatttacacc
    12361 acaaaatgta agttaaaatg tatataaaaa tgcgaacgtg aaataattat tgtaggaatg
    12421 caaattggtg tgttaaaatg gttacaattt actaagcaaa aaggggtgca ttttcctatg
    12481 caatttcttg agccaaaaaa taagaacgct aagtctgtgg attgggaaat atcagagcaa
    12541 gtaagggtga tagttaagca atacgctgaa tatgcagagc gtactgaaag cgaagcagtg
    12601 gatgaattct tattgaacat tttagacgat aagaagttta ttgaatggat tgccaacaag
    12661 cgaagtaata agcggattgt tgaaaaaatg ggtatcaaag atagagtggg ttgatagaca
    12721 atgaccaagc ttaaaagatt agccacgaag gaagatgaaa ttgtagatgt taaaattccg
    12781 atttcaaacg aagaattaaa agatagggct aaacaatatg atttattaac tccaaaacga
    12841 tttgcaacaa ggtataacaa aatgttgttc ttgccaacgt cttttaaatg gaacggttct
    12901 gaatatccca ttcaatataa ttactgcatc aatccgtttt gttgtaattt tgggaaagaa
    12961 cagcacaaat tcaaagatgt aaaagggaaa ccaagtcgat ataaaatgac tggtagttcc
    13021 aaagataaag gtcataaagg tatgtactgc aatgacaatc ctattggcag aggtgtatcg
    13081 caaaactgta cagtaacccc tttatctaat tggtctgttg ttgaagaaat taaacgcctt
    13141 atagaaatca atagcatcca agatgttgag ccagattatc aatttcataa agaaggctgc
    13201 tcggaagaag aatcaacccc attcaatgag ccgaagcaat tttacaaaag ggggaaaagt
    13261 aggggcaaat ctcaacgcta tcaatgtaag gcgtgtaaga agttcactaa tgttctacca
    13321 aaaagagaag aaaccactac ttatcaccaa caaaagaaca ccattcttcc aatgttcgct
    13381 aagatggttg ttggaagggt ttcagtcagt cgtacttgtg atattttagg aattggagtt
    13441 ggaacctact atcataagct ggagtggctg tataggcgtt gtttggagtt tttagaacga
    13501 tatgagacac aaccccttca aacaaagaaa tttaacgaga tgtggcttaa tacggacaag
    13561 atgcactact acctaaataa cgtccgtaag aaaggacaag gttcaaagaa atatacgggg
    13621 tttgaagacc ttaatatgca aacgtatata gttgtatctg ctgaggtctt atcaagatat
    13681 gtgttccgtt ctgatgttgc ctatgattgg aatatttcaa tggatgaatt aaatgaagat
    13741 acaagaaaat tcaaagaaga ccatctcaat acttttagtc gtaaaaatga taggttagat
    13801 tggtcttatt atccacaaga accatccgct aacgatagcg aaaataggaa tgcttatctt
    13861 cacgaattag ggaaaattac taataggagt agatttgtcg atggtttaaa tgttgatgct
    13921 ccttatacta caactgctca ctattggtta attaaacaaa tggttaatgc cgacgaatgg
    13981 cgaatgatta gtgatgatga cttctctatt agaaacgcct tttatagagt attcacgaaa
    14041 gaattaaggt tgagcgatgc acatcatttt atttgccaag ttaacaagac gaaaagcaga
    14101 aaacaatgct taaaagaatt cggacaagca aaagcagaac tgctcgattg gggagatatc
    14161 agaggattca aaacaaagtt tctacggact ttggcatctc attatctaac tgaattacta
    14221 acaagtcatc aattccacga agaggctatt agtaaagatg gagaaagata tcgcaaatat
    14281 gccgataacc ctatcaaaca tccattagcc actaaggata aaggatttta ttcagttgat
    14341 tgtagaacgg atttatctgc tctggaacca aacgaaatcg caaagatgct attaaatgtt
    14401 aatgaccatt cgaccaatag ctttattcaa cagataagac gttatatatc atctcttgaa
    14461 agaccattaa caacagctcg tggtgataag aagagctata tttacgctaa cttcaatccg
    14521 aagtatgctc aatttgcgat tacaattcta agaacttact acaacttttg tcgccctttt
    14581 aaatcagcgg ataaaaaggt tttgacacct gctcaaagat tagggattac agataaacaa
    14641 tttgattgga aagatataat ttatttcaaa tgacaaaaag agcttggagt aatcccaagc
    14701 tcttgtgctt tatctttact gttcttcttc aattaacgca cccgttaact caataagttt
    14761 tttctttcat atacatgaaa atagtagtta tacggatttt tatcattctt tatccctttt
    14821 tgggcaaata cctcattcca ttcctcataa ttcacttctg gaaaaaaagt atctccttcg
    14881 aattcatgat gtatttttgt gatgtacatt ttctcaacat aagggaaaaa caaattataa
    14941 atctgttctc ctccgaaaat aaaaatttct tcttcgtttt tacataactc aaaaacatct
    15001 tctattgaat gaacaatttc acaaccatta aaggtaaacc ccttatctct cgtcagaata
    15061 atatttcttc tgtcaggtaa ggctcttccg attgattcaa ggttcttcct acctaatatt
    15121 atcggatgtc cctttgtagt atttttaaca tattcccagt ccttgggaat cctccaagga
    15181 atgtcattct ctttgccaat cactctattc ttatccatcg cagcaatcaa agaaactttc
    15241 atttataaca cagccttttt aactaattta ccatctaatt tcttatttct atcaaaaaac
    15301 cttttctcat tatcctgccc gttagttgaa taagcaattt tttctataag ttatattatc
    15361 ataataccac aaaatcacaa attttcattc aattatcatt ttcaattgca ttttataaat
    15421 cattttgtat gtaatttggt atgtaagtta aaatgaaaaa agtagtccat tttgaagtac
    15481 attttgtcat tttgtactcc aaaactgaac tacttaaaat aaaaatgccg cttttttttg
    15541 ttttaaaaac cgctcgccat aatgataagc tacgagtgtt gtacacaagt tcaataacca
    15601 aacgtaaata atatagctta caatcaaatt ttttgtcatc caatataaca ttacagctgg
    15661 aaaaaaagtc agaaagggat aaaaataatt tacaccatat ccagtataat tgaatgtctc
    15721 ataattaatc ggactggtaa aaatagtcga taaccccttg attcgagata agtgaaaaga
    15781 aagatctcca gtggtataaa tactctgtgt atttttgaaa aagatgaaag aatatatact
    15841 actaactata attaatccac tcaatattac tactttttta tttattttca ttcttcttgt
    15901 ttatctcctt tttagtcaca tatcgagaat ctttcaactt attaggtgca aatcgtttaa
    15961 aacttgaata caccactaaa caataagtca gaatactgat ccataaagcc atgagagcag
    16021 ttggagtata ttttgttgta atttctatct tattttctcc agcatttaca tccacattca
    16081 ctaagccatg ttttttataa gagtcattta cgactttacc gtttactttt acctgtgtac
    16141 ctaaataagc atatactggt actgtcactt tttgcttttt gttgcttgcc actgtgtaag
    16201 taatgttatt atctgcagct ttatacttcc ctttttccca cgattctcct acttttactt
    16261 catgtttttc taagtgaggc ctttctttta tcgcttctgt tggggcgtaa tcgtataatc
    16321 ctcccatatc tgatgttgta taacgtggga gtgtttcttc attgataaca atatgaccgt
    16381 gttttgattg gataactttt gcgtaatttt cttttgctgc tatagacgtg acgagtagaa
    16441 acaccacagt agcgccaatg attaaccatg aatatttttt attcatagtc tttttcataa
    16501 aaacaagcgt tatgatcatt gaagaaaaaa agatttggat acctaagata cgccaaggaa
    16561 actggagtaa gcttatcggt gttttttgaa acaaaaacca tggaaataaa ttagttacgc
    16621 atacccatgt agaaactacg ccaacataga tgattttttc ttttttctcc atttctttca
    16681 gtatgaataa aggaaaaatc aacgcaacca atcctaataa ccccatagtg tatgttgtaa
    16741 tatcgttatt taacgcttga atgatactat ctgaaataga aagtgcttct agctgcaaat
    16801 ctgttttgaa gggactgtgt atttcttgat aattcatctg ctgcaacata ggataccaaa
    16861 aatagctagt taatgcacaa gtcaaaaaaa cagcctttac taaattcacg accacaaatt
    16921 ttatatcgtt ttttatttgc ttgcttaaaa acaaacgaac tataatcata caagcaatca
    16981 atgcactaca caatagtgta gttaagacat gagtatacac aagtagactt atactgacag
    17041 ataaagcgat gacttttttt tctctattga atatcaatcc ataagcataa tataaaacag
    17101 ctgggataat cgtaagagcg attgcttctg caattgcgga tcgataatag atatcaactg
    17161 ttcgataagt tgcaaaagta tataaacaac taaataaaaa agcagcatct atctttttaa
    17221 aaaacttttc tccataataa aaac
//
LOCUS       DACDYI010000068        17005 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.068, whole genome shotgun sequence.
ACCESSION   DACDYI010000068 DACDYI010000000
VERSION     DACDYI010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.068"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..124)
                     /locus_tag="IWM52_002215"
     CDS             complement(<1..124)
                     /locus_tag="IWM52_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAP9974444.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVF"
     gene            complement(228..539)
                     /locus_tag="IWM52_002216"
     CDS             complement(228..539)
                     /locus_tag="IWM52_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP9974445.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(541..738)
                     /locus_tag="IWM52_002217"
     CDS             complement(541..738)
                     /locus_tag="IWM52_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAP9974446.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1132..2859)
                     /locus_tag="IWM52_002218"
     CDS             complement(1132..2859)
                     /locus_tag="IWM52_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9974447.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2852..4045)
                     /locus_tag="IWM52_002219"
     CDS             complement(2852..4045)
                     /locus_tag="IWM52_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9974448.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4232..4876)
                     /locus_tag="IWM52_002220"
     CDS             complement(4232..4876)
                     /locus_tag="IWM52_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9974449.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4970..5104)
                     /locus_tag="IWM52_002221"
     CDS             complement(4970..5104)
                     /locus_tag="IWM52_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAP9974450.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5106..7238)
                     /gene="feoB"
                     /locus_tag="IWM52_002222"
     CDS             complement(5106..7238)
                     /gene="feoB"
                     /locus_tag="IWM52_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAP9974451.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7235..7708)
                     /locus_tag="IWM52_002223"
     CDS             complement(7235..7708)
                     /locus_tag="IWM52_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAP9974452.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8109..8333
                     /gene="nrdH"
                     /locus_tag="IWM52_002224"
     CDS             8109..8333
                     /gene="nrdH"
                     /locus_tag="IWM52_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAP9974453.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8492..10651
                     /gene="nrdE"
                     /locus_tag="IWM52_002225"
     CDS             8492..10651
                     /gene="nrdE"
                     /locus_tag="IWM52_002225"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAP9974454.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10701..11666
                     /gene="nrdF"
                     /locus_tag="IWM52_002226"
     CDS             10701..11666
                     /gene="nrdF"
                     /locus_tag="IWM52_002226"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAP9974455.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11755..12894)
                     /locus_tag="IWM52_002227"
     CDS             complement(11755..12894)
                     /locus_tag="IWM52_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9974456.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13203..13916)
                     /locus_tag="IWM52_002228"
     CDS             complement(13203..13916)
                     /locus_tag="IWM52_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAP9974457.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14170..14871)
                     /gene="nagB"
                     /locus_tag="IWM52_002229"
     CDS             complement(14170..14871)
                     /gene="nagB"
                     /locus_tag="IWM52_002229"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAP9974458.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15105..16637
                     /locus_tag="IWM52_002230"
     CDS             15105..16637
                     /locus_tag="IWM52_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9974459.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16828..>17005)
                     /locus_tag="IWM52_002231"
     CDS             complement(16828..>17005)
                     /locus_tag="IWM52_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9974460.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5278 a         3495 c         2909 g         5323 t
ORIGIN      
        1 aaaaaacaca ttccactaaa tttgatgccg gttttccttg aggttttgtt ccagcacctc
       61 ctttaatttt tttaagctct tgatctgaac attgtttagc atttagtcta tttttttctt
      121 ccattacgat tccccctaca aactataaat acttttatgt cactgattca atatacctat
      181 ttttctaatc aaaaaatttt gagacatatt taatattata agtatcatta aaattgagat
      241 ttatctccat aatctgctcg atataaacca tttattccag cagttcttcc aatttcactc
      301 aaatttctta ttaattcctc ttgatattca gtgaatctta ttttattagt ataagcagta
      361 aaatatagat aattcaccaa tctatttatt aaaggactgg gatctaattc atatttttgt
      421 ttttctaatt ttaaatatac cttttgtaaa acctcaagaa tatcagaata tttaggatct
      481 ttacttatag atatatcatt atataattca tgaacaattt gcttagcatt ttttttcatt
      541 ttagcacttc cctggaattg ctccacctaa aaaaccacct atagacattc ctgcaataca
      601 agtagttgcc ttggcccaat cgaccgtaca tttattttta gtgcaataca ctccatttcc
      661 ataatatttt ccactatgag tggtaccccc atagataagt tgtgtctctt taatagacaa
      721 aatttttaaa tgtttcataa tctaatcacc cctaaattaa tgtttttaga ctattattct
      781 aacattcatt aaatttcaaa aaacaattcc ttcttgaaac gtagcaaaat aattcctgaa
      841 taagaattta atcactttta agtatataaa ctatgtatat tctttaatat tttttgagaa
      901 tgcacttcgt acataataaa ctttaatcac attagtcttt catacctacg atcgatctct
      961 ttgactcgca cccataaatt cataatagtt tgtcggcgtc tttgaacgtt tcgaagatga
     1021 gctacgcatt ctcttttttt atagacaaaa aagatagaga cgaacatgga gcttccaaat
     1081 ttggatattt gatccaattt tggaagctcc atttatttcg tgtctgtctt tttatttttt
     1141 ccagctttcg actaattctt ggttctcttt gatccagtct tttgctgctt gctgtggatc
     1201 ttttccattg ttgatttcca acataacttt ttccatatct ttttctgacc aatggaaatt
     1261 gtctaatact ttgtaagctt ctggctggtc ttctttcaat ccttttcgaa ccatcgtatg
     1321 gatctgctct tcgcttccca ttccgttttc aggatcttct aagtatttca gatcatattt
     1381 ggcaaacatc caatgaggtg tccatcctgt aactacaatt ggttcgtgtt ttttgatcgc
     1441 ttgacctagt gcaacggtca ttgctcctga agaagatgtc tctactttcc agtctttcaa
     1501 gttgtcatat ttttggatcg tgttttcagc agccgtaact acaccagctc ctggctcgat
     1561 tcctatgatt ttcttacctg cctgatcggt taactcatcg atcgaattta catccatgta
     1621 ggctggcact gctaaaccaa cttttgctcc tgtcaaattg gcacctaggt cttcgacttg
     1681 atctttatat tgtgcatatt gtgaaccatg agttttaggt agccaagctg aaaccatggc
     1741 atctgactcg ccatttgaaa cagatttcca catgattgaa ttatctaatg gcgtcatcgt
     1801 tacatcatag cccatttgtt tcaatacttc accgactaca tttgttgaag caacttctgt
     1861 atcccattca acataagata agttgatacg ttttgtttct ttggcagatg aaaaactccc
     1921 tataccaatc gcacctaaga tcactactac tacagcacca atgatcagtc cttgacgttt
     1981 tttacttttc tgtgttgttg ctcctgcagc ttttttattg tttggctggt tcaaatgctg
     2041 tgtaaaacgg tcaatgatga ttgctaagat aactaatgca accccgttga caaatccatt
     2101 cccgacttgt gctctttgca atgctgataa tacgccgcgt cctaagccgg gtgcaccgat
     2161 catagaggca ataacgacca ttgaaagtga cagcattgtt gtttgattga tccctgccat
     2221 gatcgtactt ttagctaaag gcaattctag tttgaatagt ttttgacgac ttgtgctccc
     2281 gaatgaatca gaagcttcta ctaattcttt aggaacttgt cgaatcccta aattcgtaaa
     2341 acgaacggtt ggtggcagag caaagatgac tgaggcaaat acccctggaa ccatcccgat
     2401 accgaaaaat gcaactgctg gaatcaaata aacgaatcct ggcattgtct gcataaagtc
     2461 aaggatcggt ttgataatac tttgggcttt actgcttttt gccatcaaga tccctaatgg
     2521 taccccaatg atgatcgaga tcacactaga taaaagaaca agtgtgacag tattcatcaa
     2581 atcattccat agaccttggt tataaataaa ccatagaccg attaatgtga acaacgtaag
     2641 tcctggacgt ttcttagaaa taaagaaagc tgctaccgtc aataacaaga taaacaaagg
     2701 agctggaatc actaacagta aatttgtaat ccccgacatc actgcctgtc caatcgtttg
     2761 taaaaatgaa aataggccag aaaaagttgt cgtaaaccat tctgtgatat tttctaccca
     2821 gtttgccact ggtaattgat agttcaatag attattcatg ttcactcacc tcgcttgttt
     2881 ctgctaatgc ttcaatcaca ctacctcgga tgacgacacc ttttaatttt ccatcctcta
     2941 caactgcaag tggagtcggt gagtcataaa taatattgaa aatatcactt accaccatgt
     3001 ctttcccaat caccgtcaca ttttcatcta ggaagtcgat caatggacgt ttttgcttcc
     3061 tagcttctaa cgcttgatct gcagtgatga tccctttcag gtgtctcttt ctgtcaacag
     3121 ccatcagcat gctgacttct tcattacgca tccttgtcaa tgcaacgttt ggtccatcag
     3181 attcgatatt cgtcgtcaat gcaggtacca tgatgttttg agccgtaagc actttggaac
     3241 gatcgacttc ttccacaaat tcacgtacat aatcatttgc tggattggtc aatatttctt
     3301 cacctgttcc gatctgcatg atttcgccgt ctttcatcaa ggcgattctg tccccaatcc
     3361 gtaaagcttc attcaaatca tgggtaatga aaataatcgt tttttgaaca ttggcttgca
     3421 ggtccagtaa ttcgtcttgc atttctcgcc ggatcagcgg atcaagagcc gagaacgctt
     3481 cgtccatcag caagatctca ggatcatttg ctaaagctct ggctaggcca acccgttgtt
     3541 gcatcccacc agataactga ctaggatatt ggtctttaaa tgaaagcaaa ctagaatttt
     3601 ctaatgcttt ttctgctttt tcttgtcgtt cttctttagg tacaccacgg acttctaacc
     3661 cgtattctgt attttccaag atcgttcgat gtggaaacag accgaagttt tggaaaacca
     3721 tattgatttt atgacgacgg acttctcgca attcttcttt gcttaacttc gatacatctt
     3781 ctccatcaat atagatactg cccgaagttg gctcaatcag acgattcaat agacggatca
     3841 atgtggattt cccactcccg gaaagcccca tgatcacgaa gatttctcct tcttttacgt
     3901 caaaattcac gtcatacaca ccaaccgttg caccggtttt ctttaaaatc tccgttttcg
     3961 gctggtgatc attcatcata tctaaagcgg cttgtgtctt tttaccaaat atctttgtta
     4021 aatgttttac ttctactttt accaaacaaa ttctcctttt ttatgtaaat tcatttttct
     4081 ttctgaaaaa agtgaccaca ctatgttaac atcatgtagt cactttgtca aaaatgaaag
     4141 aaatttgctt gtccaaactg aaaaaaactt ggctaaatct ttgtaaataa aaggtttagc
     4201 caagttaata tttttttaat atttcacaca atcattctgc aagctgatgc tccgggtaaa
     4261 aaaagtttgt catacgctgt agtgtatatt catttccaat aaaatgaacg atgtcccctg
     4321 ccgtaaattt agcatagggg ccaggtgaaa caagcagttc ttcattatga gaaattgcga
     4381 taactgtggc gccagtcgcc tgccagatat tcaactcgct aattgaacgg tccagatgca
     4441 ttgctttttc tgataaagat aattcaaacg gaagtaaagg attcatctta tgtattcgct
     4501 tcgtttgatt gaccagctga tccaataatt gagaaaaatg attcagctca tcttgttgct
     4561 tctccacact tgccatgatt tctgcttttg tatcttggat ggattgtaca tcttgatatt
     4621 gcaataggaa atcttttgct ttttccttcg aagcaacaaa tgccccactt ccatgccgta
     4681 cttccatgat tccaagatct accagcacat tgatagcctt gcgggtagtc tcaggagaag
     4741 cattgtacgt actggcaagt gttgagcggg catggatctt ctcacccact ttatatcggt
     4801 tttcaacgat acgttcggct aaatcaacag caatctgctg gtagcgcggg cggacgattt
     4861 ttttctttcc agacattttc tttcctcttt ctttttttac tttccctcat cttactatgc
     4921 tctagcaaaa taagcaaaaa aggggagaat agcctcttgc aacgactgat catcgcttga
     4981 tctgttcctt ttttactggg caagctgttt gacagtcatc acaattctta ccggatttta
     5041 aacgagtata gacaattgcg cctgcacttc caaagattaa cacacttaat aagaatgtcg
     5101 ccatcctatt cctctccttc taatgacaat atagtataaa actggttctt ggattttggc
     5161 tgacggaaca aaacataaac gagtcctatc atcagtaaaa tagcaagata cgtccctaac
     5221 gctacctgtc caccctccaa taagtgtccc aactgataaa tcacaaaact aactaaataa
     5281 gctaaaccgc attgatagcc tatggcgatc caagtccatt tcttggcatt catttcccga
     5341 tggatagccc caattgccgc aaaacaaggc gcacaaagta aattgaaggc taacaatgaa
     5401 taagctgctg cgggagtata tgtgtgttgt agcacttgcc agatttctct gccattttcg
     5461 gatacttccg catgaccaaa caaaatacca aatgtgccaa taatattttc tttagcgacc
     5521 aatcctgtaa tcgctgcgac tgttccttgc cagtttcccc aaccaagcgg ctgaaaaaca
     5581 actgccagga ttcgcccgaa aaaggctaag atactttggt cttcagaaac aggttgagca
     5641 taaaaattat aagaagagct gaaccagatc acgatactag aaacaaagat aatcgttcct
     5701 gcttttttaa caaaagactt gctgcggtca taggtttgtc ttaggaccgc tgaccattga
     5761 ggaaaatgat atgcgggcaa ttccatgata aaaggagctg tatctccagc aaacaaacgt
     5821 gtttttttca agctgattcc agagaaaata attgcagtca taccaagaaa ataggcagaa
     5881 ggagctaccc aagattgatg aggaaaaaag gcccctgcta ctaaggcaat aatcggcaac
     5941 tttgcagaac aaggcatgaa ggttgtgacc atgatcgtca aacgacgatc ttgctcgttt
     6001 tcaatcgtgc gactggccat gacgcctggc actccacatc ctgtagcaat cagcatagga
     6061 atgaaggatt ttcctgaaag accgaatttc ctgaaaagtc gatccatgac aaaagcgatc
     6121 ctcgccatat atccgcagtc ttccagaaag ctcaagcata aaaacaatat aatcaattgc
     6181 ggtagaaatc ctaacactgc tcccacgcct gcgatgatgc cgttcaagat cagctcctgc
     6241 atccatcccg ctacttgcca ttcagctaac aatctgccaa catgttctgg taaccatgta
     6301 ccaaacaatg tgtcattgat ccaatctgtc cccattgttc ctactgtttg aatagccaga
     6361 taatagatca gccacatcac aaatgcgaag atcggcaagg ctaaccaacg attcgttgca
     6421 atctgatcga ttttgtcact catcgataat tggaaagaat catttgacac aacacagagt
     6481 gtacatagtt gggtaataaa ttcataacgt tcattgacca gaatcgtctc actatcgtcg
     6541 tccaatagtt tctctgttaa ctggatcagt tgctcgatct ctttttgctg agaaactgct
     6601 atctcgtaat cctctcttac cttctgatct cgttcaaaat atttcattgc ataccagcgt
     6661 ttcttgccgc tttgttctcc tagtacatct tcaatttcat gtaaagctgc ttctaaacgt
     6721 ggatcatatg tacagggaac aggaatagaa ggccgctttt ttgcagactc ctgaatcttt
     6781 tcgattagtt tttcgattcc ttgctttttc aatacgctga ttggtaaaac tggtacacca
     6841 agaccatagg aaagcttttc tagattgatt ttttttcctt cttttttgat gacatccatc
     6901 atattcagac caatgatcat aggaatgtct aaatccaata actgcgtagt taaataaaga
     6961 tttcgttcta aattggtagc atcaataatg ttgatgatca gttcaggctg ttcctgaacc
     7021 aaataatctc ttgcaatcac ttcttctggt gtataaggag acaaagaata gatgccgggt
     7081 aggtcctgaa tatgaatgtt cggatttttt ttcaacgatc cttctttttt ttctactgtt
     7141 acgcccggcc aattccctac atattgattc gaaccagtca agagattgaa caagctagtt
     7201 ttcccgctgt ttggatttcc gactaacgcg atattcattc tttttcctcc ctttgtatca
     7261 agattagttc ggcctctgac tttcgtaacg tcagttcata ccctcgcact gtcagttcga
     7321 ttggatctcc cagaggggct agttttcgga taactatttt tacatttcgc gtcaatccca
     7381 tatccattaa ccgtcgcttc actgcgcctt gtccgtggat tgctacgacg atcccagtct
     7441 ctcctgcttt taattgatca agagaagtcc agctttcaga ggaagcagtc tttttattga
     7501 caaagatttg acctagtatt tcccgactta ctgccactcg atgacttttt accagtaaaa
     7561 tggcattgtt gcctgataaa ttgacgacga cgattttact tccggcaacg atcccgatat
     7621 tttgcaaatg gcgttttgtt ttttcttctg tttcgattcg ttcgacttga taaacgaaat
     7681 tctgctttgc ctgcgctaag gtctgcatat tgattcctct cttgtgaaat aaatcatgta
     7741 ttaaaatcat tcattatatg ttctaatttt cttacgtgtt acgcaaataa atgagaatca
     7801 ttctcacata gaatactctc ggtaattgag aatgtcaatt atacttgtga tattatgagc
     7861 gaatctttag tattgataga taaaaatgag aaaaaaatat ttcttcatag ttttccacaa
     7921 ttttttctct attaaatttg ctattttccc acacgatact tgttttcagc tcttttttca
     7981 aaaacacaaa acttagtatt ccacagtttc cacaacacaa tatgtagtgt ttgttgtttg
     8041 acttttttac taagagtttt atactatcta ttatagataa taagaatgtg ttagaggagg
     8101 cactcaccat gaacgtaaaa atcttttcta aaaataattg tatgcaatgt aagatggtca
     8161 aacggttttt aacagaaaat caaattgctt ttgaagaagt caatatcgat gaacagcctg
     8221 aagcagtaga ctggttgaaa gcacaaggat tccaaagtgt tcctgtcatt acttctgatg
     8281 cagaaacagt tatcggattc cgtcctgatc agttgcgtaa acttgccagc taacatgact
     8341 aaaagaatca aaacgtgata catggatttg aagggcttga gacagcacca gtttttgtcg
     8401 caaacttcca aatccttttt taaagcttaa aaacaatgca atgatttgca atcaagcatc
     8461 atcaaatacg aaatgaaggt tgggcgtacg tatgagtctt aaaaatttac aagatgtcag
     8521 ttatttcaag ctgaataatg aaatcaatcg gccagtcaat ggtcaaatcc ctttgaataa
     8581 ggataaagaa gctttagttg ccttttttga ggaaaatgtc aaaccaaata cgatgacttt
     8641 tccatctatt atggaaaaaa tcaattactt gatcgatcaa gaatatatag aagcagaatt
     8701 cattcaattg tattcaccat catttattga aaagctatat caatttttgg acgagcaaca
     8761 tttttctttc aaatctttta tggctgctta caaattttat tctcaatatg cgttgaaaac
     8821 caacgacggt tccgcttatc tggaaactta tgaagatcga gtagccttca atgcgcttta
     8881 ctttgcgaac ggggatgaag aattagcact tgctcttgca gacgaaatga tccatcaacg
     8941 atatcagcca gctacacctt ctttcttgaa tgctggacgt aaacgtcggg gcgaattggt
     9001 gtcttgtttc ttgactcaag tcactgacga tatgaactct atcgggcgct caatcaactc
     9061 tgctttgcaa ttgtcccgta ttggtggcgg cgtagggatt actttatcaa atcttcgtga
     9121 agccggcgct cctatcaaag gatatgaggg tgctgcaagt ggtgtcgttc cagtcatgaa
     9181 attattcgaa gatagtttca gctattctaa tcaattaggt cagcgtcaag gagctggcgt
     9241 ggtttatttg aacgttttcc atccagacat tattgctttc ctttcagcaa aaaaagaaaa
     9301 tgcagatgaa aagattcgcg taaaaactct ttctcttggc gtgatcgtac ctgataaatt
     9361 ttatgaattg acacgtaaaa acgaagatat gtatttattt agtccatata gtgtcgaaaa
     9421 agaatatggt gtcccattct cttatgtaaa tattacagct gagtatgatc atctagttgc
     9481 aaatccaaac attcgcaaac aaaaaatcaa agcgcgtgat cttgaaaatg aaatctcaaa
     9541 attacaacaa gaatctggct atccttacgt aatcaatatt gatacagcga accgcgagaa
     9601 tccaatcgat ggcaagatca tcatgagtaa tttatgttca gagattttac aagttcaaac
     9661 gccttctgtg attaacggca aacaggaata tgaagtatta ggaacagata tctcatgtaa
     9721 cttaggctcg acaaatatcg tgaatttgat gcaaagccct aactttggtc aatctgtgcg
     9781 tacgatgact cgtgcattga catttattac agatgcatct gatatcgatg tcgtaccacc
     9841 gatccaaaac ggaaataagc tgaatcatac gattggatta ggtgccatgg gcttacacac
     9901 ttatcttgca aaagaacaaa tggaatatgg ctcagaggat tcattagact ttacaaatat
     9961 ttacttcatg ctgttgaatt attggacact tgttgaaagc aacaatatcg ctcgtgaaag
    10021 aaaacaagtt ttccataatt ttgaaaaatc agcttatgct gacggcactt atttcgaaaa
    10081 atacgtaact ggagaattcc agccaaaatc agaaaaagtc aaagcattgt ttgaaggcat
    10141 ttttatccca actgctgaag attggaatgc tttgaaacaa gcagtaatga aagatggact
    10201 gtatcatcaa aatcgcttgg cagtagcacc aaatggctca atttcatata tcaacgatac
    10261 gagtgcaagt attcatccta tcactcgcat gatcgaagaa cgccaagaga agaaaatcgg
    10321 taaaatttat tacccagcgc cttatctatc caatgagacg attccgtatt acacttctgc
    10381 ttatgatatg gacatgcgta aagtaatcga tgtttatgca acggcacaac aacacgtcga
    10441 tcaaggcatg agtttgactt tattcatgcg ttctgaaatc ccagaaggca tttatgaatg
    10501 gaagacaaca acgaagcaaa cgacacgtga tttgaatatt ttaagacatt acgctttcca
    10561 caaaggaatc aaatccattt attacgtacg tacctttact gatgatgcag aagaaatcgg
    10621 aagcaatcaa tgtgaaagct gcgtgatcta atatcataga tagattcgct tattcatatt
    10681 ttaattaaag gagcattctc atgtcagaaa catattatga agcaataaac tggaatgaaa
    10741 ttgaagatat cattgataaa tccacttggg agaagctgac agagcaattt tggctagata
    10801 cacgtatccc actttcaaat gacctagatg attggcgtac attgtctcaa ttggaaaaag
    10861 atacagtagg acatgtattt ggcggtttga cactgctaga tactgtccaa tctgaaagtg
    10921 gcatggatca gttgcgtaaa gatgtccgca ctccccatga agaagccgtt ttgaacaata
    10981 ttcaattcat ggaatccgtt catgccaaaa gttattcttc tatttttagt acactgaata
    11041 cgaaaaaaga aatcgaagaa atttttgact ggacaaatac gaatccttat ctgcaaaaaa
    11101 aagctgagcg tatcaatgaa atctacaaac acggtacacc gctagaaaag aaaattgcca
    11161 gcgtcttttt agaaacattc ttgttctatt caggttttta tactcctctt tattatttag
    11221 gaaacaacaa attggctaac gtagcagaga tcatcaaact aatcatccga gatgaatctg
    11281 tacatggaac ctacatcggt tacaaattcc aattaggatt caacgagtta cctgaagcag
    11341 aacaagaaac gttaaaagac tggatgtaca atcttcttta tgagctttac gaaaacgaag
    11401 aacgttatac agaagaatta tacgatccaa tcggctggac agaagaagtc aagacattcc
    11461 tacgctataa tgccaacaaa gcattgatga acctagggat ggatccactc tttccagata
    11521 ctgccaatga tgtcaaccca atcgtaatga acgggatttc aacaggcaca agcaaccatg
    11581 acttcttctc acaagtcgga aacggctatt tgttaggtca tgtagaagcg atgaaagacg
    11641 acgattattt gattggattg gaataaggat ttattttaat tcaaaaattt ttcgaaaaat
    11701 aaaaagagtt tagcgtacag atattttaaa tgtacactga actctttttt atgcttattc
    11761 acttgtttct tccagcactt ttggactgtc taacttttcc gaaacatgtt ccattcttct
    11821 caataaatag attttccgga tatttaatat tatcgtctta atcactgcat agacaggtat
    11881 gcaaatcagc atgcctaata ttcctgcgac acttcctgct ccaatcaata ccaagatgat
    11941 cgtcaaagga tggatattca ttgatttccc aaataacaac ggcttgatca aatttccatc
    12001 taattgttgg atcactaaaa tcgacaacgc catatacaaa gcttgaaatg gagagataaa
    12061 caaaccgaca atgattgctg gtaccgctcc aataaaaggg ccgacatacg ggataatatt
    12121 cgtgattccg caaatgaccg caagcaacaa gctataaggc tgatcgaata ttctcattgc
    12181 caaaaagctc attacaccaa taatcaaagc atctaagatc gtactgctga tataggcaga
    12241 taatgtttcg tttaattctt ttaccgtttg gcgtaactct ttacgaatcg ctttcggaaa
    12301 gaagtgagac aacgcatcaa taaatttgtg cccatccttg aacataaata gcaaaataaa
    12361 tggaacggta aataacaaaa taaaaaattg catcataaat gaaatgattt ttgagacact
    12421 tgcagataaa ctgactaata tgatattgaa caaattacta agagataaat ttgctgatgc
    12481 taattgttct tccaaattga aatttttaaa ctcttcccgc tgcgccaatt catttaacca
    12541 tttgcttgtt tcatctgcat acattggcaa gctctgggtc aattgaattg tctgttccat
    12601 taactgaggc actacattca tgacagttaa aacaactaaa atgattacga ccaaaaacgt
    12661 caataaaaaa ccgaacactc tcgggatccg atgcttttcg agaaaaacaa cgattggatc
    12721 gaacatataa tagataaaac cagcaacaag caatggtaaa aaaattgccg aaagcatttt
    12781 caatattgga ctaaaaatat tcggcatttg taaaataaag tacacgccga tgatcgtgag
    12841 tactagctcc actgtccaga aaaataattt tgattgttta aaacgtgatt gcatacatct
    12901 cctccaaggt cctttgatac ttctattcta tcatttaaca aagagttacg cgctgagatt
    12961 ttcatttgct taaaaaaaac aaaaaaaata aacatatttt aatgagtaga cgagtactta
    13021 aacaaaaaca cggtttggag tagtgacaaa aattatactg cagtcccttt ttccaaataa
    13081 acggtgttca aaagcttgca tcttcgtaat aagcccagcc aaacgaaaaa tataaggaac
    13141 tatttttatt tggctgggct tattacttga tgtaggacat ctattttaca acctcagatc
    13201 atttattgcg caatactgac taaatgcaac tttgtgatat ttttgtgtac ggacaactcc
    13261 tcgatcacat cggcataagt gagccctttt ggaagatcaa tcgtatagat atttttgtac
    13321 actcggtagt catcaacgaa ttttacgtca aaattcacat cttcgattac gatatttttc
    13381 tcttcaaaat aatcggtaag aaaatctttg gtttcttttc tatggataaa ttggatttct
    13441 aactttttag tcgtaggtac atggatcacg cgtttcacta ctgtgagtgc aatagcaatc
    13501 ccgataaaac tgatgatcgc aatggaatag taacccatgc cgatggcgat ccctaatcca
    13561 gcaattgccc ataaagaagc ggcagtcgta agtcccgtca ccgattgttt cgtgacgatg
    13621 atcgttcccg cgcctaagaa accgacacca ctcaccactt gagcaatcag ccttgcttca
    13681 tctgaacgaa tcgttccagc aagctgtgga tactctaaag catctcttaa tgcaccacta
    13741 gcgatctcaa cttggatcaa tgcaatgata catgctccca tacaaactaa aatatgcgta
    13801 cgcatacctg ctggacgatt tttatattgt cgttcaaaac cgattgctcc gccaaatagc
    13861 atagcgatcc ctaaacgtaa aataatctct ggaattgtca ataccatatc attcatctaa
    13921 acaccctcca gtccaatata tcatgtctga aaggagcaga caaaaaaagt gcaattttta
    13981 cctcttacga aaaaaggact gtgacaaaat ttttgtcata gtccttttta tgaataaacg
    14041 gtgttcaatc agctgaccct cggtattact caacaaacgg caaaatatgg ggagctattt
    14101 ttcctttgtc ttctctaata ctcaggtcaa aacgctgatt tcacaacctc tttagcctac
    14161 tattgaaaat tacaacttac ttgctgcttc ttcatcgatg atcacaacaa cattgtcgtg
    14221 attttgcaat acacttgctg gcaaatgatt cgttacaggt ccttcaatca tccctttgat
    14281 tgcatcagct ttgctttcgc caaatgccat caagacgatt tctttacttt tcataattga
    14341 accgattccc attgaaacag cttttgttgg tacatcttct gctttatcga agaatcgttt
    14401 gtttgcattg attgttgatt ctgttaagtc aactactgaa gtcgttccgt caaatgaagc
    14461 accaggttca ttgaaaccga tatgtccatt ttgcccgatt cctaagattt ggatatcgat
    14521 tggatgtgca tcgatgattt gatcatactt tttgcatgct gcttccagat cttctgcttt
    14581 tccatctggt acaaacgttt ctttgaatgg ttttttatca aataattgga catccatgaa
    14641 atgacggtag ctttgttcat ctgaaccgcc tagtccaacg tactcatcta agtttacaga
    14701 agtcatttca ctgaaatcca aatcgcttga agtcatttct ttatataaag taactggtgt
    14761 gcttcctgtt gccaatccta atacttttgc acctttgttc ataccttctt tgatgatttc
    14821 aaatgctttt tttccgcctt cttcagcgtt ttttacacga atgatttcca tagtgattgc
    14881 tcctttatat tttggtatag cccaattata gcattctttt tttcttttta caataattag
    14941 tatagaccaa attgcatttt tttcattttc ttgacaataa ataggaatcc gtgttaggtt
    15001 tcataagatg gttcaatcaa acaatccatg ttgcatgaaa accaagcaag tgattcactg
    15061 tggacgcttt atgtctgcag tttttttatg aaaaaggagt tattatgagt atactgacat
    15121 taaacaacat tacccagcaa tttggcgaca aaatcctcta tgaaggtgtc gatctgcaat
    15181 taaatgctgg cgaacatctt gggctgattg gacaaaacgg agccggtaaa agtacattga
    15241 ttaaaatcat cactggtgag atcttaccag atgacggaca gatccattgg caaaaaaata
    15301 ttcacaaagg ttacctcgat caatatgtgg aagtcgatga aacattaaca atcgaagaat
    15361 ttctaaaaac tgcgtacgca aaagaatacg aaaaggaaga aaaaattgct tttctttacc
    15421 aaacatatag cgagaccttc gatgattcct tgctggaaaa agctgggaaa ctccaaacgg
    15481 aattagacca aggagtgttt tatcaaatcg acaccttagt tgctgagatg agcagcgggt
    15541 tggggatcga tgtcttaggt ttagattcac tgttgaaaaa tctaagtggt ggacaacgtt
    15601 caaaggtgat tttagcaaaa ctacttttag aaaacccaga tgttctgatc ttagatgaac
    15661 caaccaatca cttagatgat cagcatatcc aatggctgac gcagtttctt caggattttg
    15721 acggtgcgta tatcgtgatt tcccatgacc aagaatttct tgatcagatc accacccata
    15781 tcgcagacat cgagtttgga aagattacta agtatactgg ccatctaaaa caagcattga
    15841 aacaaaagga acaaaatcgc gaaagctatt tgcgccaata tcatgctcaa caaaaacaaa
    15901 tcgaaaagac cgaagcttat attcgaaaat acaaagccgg ttcccgatca acgatggcaa
    15961 aaagccgaca aaaacaatta gataaaatcg aacggctgac gcctccagct tcagcaagta
    16021 aagctgtctt tgattttcca ttctcgccta ttgtcacgac attagcagtg gaaacaactg
    16081 atttagtcat cggatatgat cgaccgctac ttgcacccat caatttgacc atccgctttg
    16141 gtgaaaatat tgccattcga ggcttcaacg ggattggaaa atctacttta ttaaaaacac
    16201 tgatcggtga gattgaaaaa ataagtggcg atttccactt cccagataac acaaaaatca
    16261 attacttttc acaagatctg tactgggaaa atccattaga aacaccacta caatatttaa
    16321 gcagtcaatt tccaaaagcg actatcaaag aattacgtcg ccaactagct aaagcagggt
    16381 tagttaatca attagcaagt gaacctctca ctacattaag tggtggggaa caaactaagg
    16441 tgaaacttgc acagttgaca atgaaccaag gtaatttact gattctcgat gaaccgacga
    16501 accatatcga tcaagaaacg aaagaaagct tacaagacgg gatacagaaa tttccaggaa
    16561 cagtaatcat cgtctctcat gaacaagaat tttatcaaga tcttgtgcaa cgagtcattg
    16621 agattgaacc aaaataaacg tttgattgaa tagttgaaaa aaaggagctg tgacaaaagt
    16681 tttgtcacaa ctccttcttc tgaataaatg gtggccaatt agctgacctt cggtattagc
    16741 tcaaaaaacg gcaaaatatg aggagctatt tttatttggc tattcttatt acttgatgca
    16801 gaacagcttt tcacaacctc ttttcgtcta ttttttattt tcgagatttc tctttccttt
    16861 aaaaacaact gtttgaaaga agaacgcaca tccttgaaaa acaagcccgc taaccgttac
    16921 acctaaccag ccatactgct gccacattgt cgtccctagc aatgaaccag ccgcacctcc
    16981 aataaagtac ataaacataa ataca
//
LOCUS       DACDYI010000069        16356 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.069, whole genome shotgun sequence.
ACCESSION   DACDYI010000069 DACDYI010000000
VERSION     DACDYI010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.069"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(141..797)
                     /locus_tag="IWM52_002232"
     CDS             complement(141..797)
                     /locus_tag="IWM52_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP9974461.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            990..1268
                     /locus_tag="IWM52_002233"
     CDS             990..1268
                     /locus_tag="IWM52_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974462.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1287..2495
                     /locus_tag="IWM52_002234"
     CDS             1287..2495
                     /locus_tag="IWM52_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9974463.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2643..3008)
                     /locus_tag="IWM52_002235"
     CDS             complement(2643..3008)
                     /locus_tag="IWM52_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAP9974464.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3155..3928)
                     /locus_tag="IWM52_002236"
     CDS             complement(3155..3928)
                     /locus_tag="IWM52_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9974465.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(4102..4962)
                     /locus_tag="IWM52_002237"
     CDS             complement(4102..4962)
                     /locus_tag="IWM52_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAP9974466.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(4962..6380)
                     /locus_tag="IWM52_002238"
     CDS             complement(4962..6380)
                     /locus_tag="IWM52_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9974467.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6437..6886)
                     /locus_tag="IWM52_002239"
     CDS             complement(6437..6886)
                     /locus_tag="IWM52_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9974468.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(7331..7594)
                     /locus_tag="IWM52_002240"
     CDS             complement(7331..7594)
                     /locus_tag="IWM52_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAP9974469.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(7693..8988)
                     /locus_tag="IWM52_002241"
     CDS             complement(7693..8988)
                     /locus_tag="IWM52_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAP9974470.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(8985..10256)
                     /locus_tag="IWM52_002242"
     CDS             complement(8985..10256)
                     /locus_tag="IWM52_002242"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP9974471.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(10421..11290)
                     /locus_tag="IWM52_002243"
     CDS             complement(10421..11290)
                     /locus_tag="IWM52_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAP9974472.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(11582..13192)
                     /locus_tag="IWM52_002244"
     CDS             complement(11582..13192)
                     /locus_tag="IWM52_002244"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAP9974473.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(14385..15725)
                     /gene="glnA"
                     /locus_tag="IWM52_002245"
     CDS             complement(14385..15725)
                     /gene="glnA"
                     /locus_tag="IWM52_002245"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAP9974474.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(15753..16145)
                     /locus_tag="IWM52_002246"
     CDS             complement(15753..16145)
                     /locus_tag="IWM52_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9974475.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         4666 a         3469 c         2781 g         5440 t
ORIGIN      
        1 ctcacaacct cttggtaaac ggtgttcaat cagctgaccc tcggtattag ctcaaaaaac
       61 ggcaaaatat gaggaactat ttttccgttt gtttctctaa tactcaggtc aaaacgctga
      121 tttcacaacc tctttgttca tcaaaagata atattgtctt ctaacaagtc catattcaaa
      181 ataacgatat gatggtcttt aacggtgata ataccatctt ttttcagttg ttttaaaatt
      241 ctgtttacac tgctagcagt tgagatgcca caaaatttgg cgatttcttc attcgtgatc
      301 gaaaaatcga tcagcatccc ttcttctgtc cgcacaccga ataaatcata taattcgtaa
      361 agttgcgtac aaacagctcc cagctttccg tttaatagca tttgctgcat ttttttcatc
      421 gaataaagca gacgatagcg gtaatactct tttacatagt gttgtagctt tgggtcttgg
      481 ttgatatttt gccaaaagcg cacacgattg attcgataaa actctgcata ttctgattca
      541 actcgaatat tatacggcga atcgatgaat tgtgaatatt cgtcttttaa aagagagacg
      601 atttcaagat ctttgatata gcgaaggtta aattctctcc catcatttga aataacactt
      661 gcttttaccg ttccctcttt caagatatac gtatgggaat cttccatccc ttcgaatgtc
      721 aaatatttat gatatccttt gcgtataatc ggaaaatcat ttttatccaa atattccttt
      781 aagactctac tatccaatga tgctccccca tccaaacttt catctaaaaa gatcatacag
      841 tactttaaaa ctttttaaag tccaaaggta ttttactaat gatattaaca tttgttaagt
      901 acctttttcc atcacttatg cgattttttt cctttttttt attcgttata ctgcagaagt
      961 aaaatttttt aagaatagag ggaaaaaaca tgaattactg gatgaacaca atcattaatc
     1021 gattagaaac agcatatcaa acaagatttg atatgaaagc ttctttagtc tttttaaatg
     1081 atgcgtatca aaactcgatc gaattgatca aagcagtgga tgagaatcca acaaacgaat
     1141 gcgaagaatt tttaaatctg tttatgtcta caagagattt atttattcgc caacttgttg
     1201 atcgctatcc atccaattat cacgatgtag aagttcaaat acaaaaatta aaagcatatt
     1261 cagcttaagg ggaatgtata acatacatgg aaaagaaatt atttaataaa tattttattg
     1321 gaatcacatt gatcaatttt attgtctacc tgatttatta tctgttgatg gtcatcatgg
     1381 cagtcattgc gcaagacacc ttacacgcca gcttgagcca agcaggtttt gcttctggga
     1441 tctatatcat cggaacattg ctggcacgct tattcatggg gaaacagtta gaactatttg
     1501 gtcggaagtt taccttaaga ggcggcgcga tcttctatct cctttcaaca ttagcatatc
     1561 tttatacgcc aactatcggg gcattgtatt tagttcgatt cttgaatgga ttcggctacg
     1621 gtacagtttc tacagctaca aatgctatcg ttactgcata tatccctgca tctaaaaaag
     1681 gggaaggcat caattattat ggattaagta ctagtttagc tgccgctgtt gggccatttt
     1741 taggaatgat tttgctgaat atgactgatt ttcgtttcat tatctggttt tcaattgtac
     1801 tggtcttctt tgttacgatt gcctgccttg tctttcctgt aaaaaatatt ccattatctg
     1861 aagaacaaaa aatatctctt cgctcatgga atatcaatag ttttattgaa aagaaagcac
     1921 ttttcattac aggaattgcc tttttgatgg gtctttctta ttcaagtgtt ttatcatttt
     1981 tatcttctta tacaaaagta atccacttag ttgctgctgg gtcatttttc ttcgtcgtat
     2041 atgctttggt catcacattt acacgtccat tgactggaag gattttcgat gtcaaaggag
     2101 aacaatacgt gatgtatcct agctatttat tcttaacagc aggattattt ttactgagtg
     2161 taacgacgaa tagtgtgaca ttgcttatct ctggagcatt agtcggccta ggttatggta
     2221 cgtttatgtc aaatggacag gctgtttgtt taaaaatcgt ccgcgaacat cgaatcagta
     2281 ttgctttatc tacctacttt attggtttag acctaggctt aggcgttggt ccttatttga
     2341 tggggatgct aaaatctctt acttcattta gaggattgta tgtgattgcc ggagtcattc
     2401 cattgatatg tacagcactc tatcttgtta atggccgaaa acatagtcat gaagcagagg
     2461 gcgaattgat tgaatcattg aaaaatgaaa tttgatcttg atgaatagat aaaaaaaacc
     2521 gttcagatct gaaaataatc tgagcggttt tttgagtaaa aatagtttat gattattctt
     2581 aattaaacat ctatttattt ttaaagcttt catttatcga taagaatagt ttaactgttg
     2641 atttattcca ttttccgttc atctggtacg atgccttgac ttagcaacca cttgtggaag
     2701 aaccattctg ccacaccgat caatagagca ggaatcaata ctttcatcca aatatcgact
     2761 gtatcattca taaaatatag atagagccac aagataactg cagctgaacc agcatctgcg
     2821 atcgtcgctg cgatgttccc tactttacgt aagatcaaca gatcccctag cacatagatc
     2881 atgactacaa taattacccc aacaatcgtt gaatccataa agctaacacc gtataaacct
     2941 gtcagcacga cccacagaat cgcccagatc atgattgcct tcactaccaa cgccttcaca
     3001 tgtttcattt tctgcactcc tttctcctgg ataaaaaagt aagattatct aacaagataa
     3061 ccttttcatt attataccat cggctgagag aagcgagcaa agataaggtc ctatctaaaa
     3121 atgaggaagc cacttagaac attcacgtaa taatttatcc gtacagacat tcgatatggt
     3181 gttcctttac tttcgcatac tgttcttttg tcagttcttt acttgtcacc agataatcga
     3241 tttcatgaaa agcaatggct tgatgcatga atttatgtgc aattttttgc tgtgtaacta
     3301 aaacggcaag ccggtctgat tgttttctaa aagctttttt gaccattgcc tctccttcat
     3361 gagtttctgt tactccttgg ctgaaatcta gcccgcgaca actcattatc gctaaatccg
     3421 cataatatga caatgcatcg ttataggctt ttgaaccgtt gattgttgct cgtttcggaa
     3481 caattgcccc tcccaataca gatacttttg ccgttgtata ttcactcaac atagcagctg
     3541 tgacgatgcc attcgtaatg atctgcaaac catcaaatct tgctaggttt ctgactaagt
     3601 gtaaagaggt tgtactggaa tccaagaata agaccatatc atctcgaatc aaagaagaag
     3661 ctgtatctgc aatcttacgt ttttctgttt cttcttctaa tttggtgatc ttcatcaggt
     3721 aaggcacgtc aatttttcga ttgtcaataa gcatggcgcc accgcgaact ctacggatca
     3781 atccactttt ttccagaaaa acaaggtcac gtttaatcgt ggcttcgcta taatttacta
     3841 actcgatcaa atcggtgact cttgcaaatt ggttgttttt tagataattc acaatctcct
     3901 gctgtctatt ctccacttca ttcaacataa aatcacccca tttactttat atagtaacag
     3961 gaaaaaagag gttgtgacag aagtgattgg cttcgagaaa taagaagaaa tttttcgaaa
     4021 aaactacttc tgaacatcgt gtgtggttaa gagggtatga caagactatt gtcataccct
     4081 cttgaccaca catttttatt tttagtataa actagccttg cctactgtgt tcaaaatttc
     4141 cattttatgt ttgaccactt cttgtacctt tttattagct gctggataaa ttgcattcgg
     4201 ctcgtacatc gatggatttt cactcaatat ttctctcaaa gcattgaaaa agacgctttt
     4261 aagatctgag ctcaaattca ctttggcaac tccgtatttt actgcttcgc tgacttctgc
     4321 gtccggatta cctgaaccgc catgtaaaac aaatggaaga tcgatttgtt ggttcaattc
     4381 tttcagtaag tcgatattta attttggagt tctgtttttc ggatacaagc catgcgctgt
     4441 tccaattgct acagcaagtg tatcgattcc tgtttctttc acaaaaatct tcgcttgatc
     4501 aggatctgta taaatgattg tttcagcacc tccttcagca gaaccattat tcccaatcgt
     4561 tccaagctca gcctccactg agacatttac cgcatgagct aacgcaacca cttttttcgt
     4621 aatctgtaca ttgtcgtcga atgatttcaa agacgcatcg atcatcacgg aagaatagcc
     4681 gtttcgaatc gcccgtaaga catcttctat cgtaccgcca tgatccaagt ggataacgat
     4741 cggcagttta cttgtataag cataggctct tactgccgca acaaactcat cacttaaata
     4801 ggcgatttca tcaggatgaa tttctaagat tactggtgct tctaatctct ctgcttcttc
     4861 gatgattgct ttcatcatat catagctgca aatattgaag gctggtactg caaattgatt
     4921 ctctttagcc actttcaata actctttcat tgtatataac attacgcttc cacctcattt
     4981 gttagatttt catttttcgt ccacttgtct tgttttttct ctttcactgt ttttttcaat
     5041 agtccaagca taaaggctgt agtgactgta cctgatgcaa ttgccaagac atacataggt
     5101 aaattgttca taacatttgg aattagcatc acccaaatcc ctccatgagg tgccattgaa
     5161 gtaactccta ctgccatgga aatccctgca gcgactgctg aacctgtcat caaactaggt
     5221 atcacgcgga tcgggtcaga taccgcaaac ggtatagcac cttctgtaat atacgaagcg
     5281 cctaaaatcc agcatgattg acctgaagtc cgttcttctt tggtaaattt atgtttgaat
     5341 aataaagtgg ctaaagcaat acctagggga ggtgtcattc ctgcagccat acaagcagca
     5401 atcggtgcat ttactccagc tgacatcaaa ccgatcgaga aagtcgaagt cgatttattg
     5461 atcggcccac ccatatcagc tgccatcatg atcccgataa gtaatccgaa caatagcccg
     5521 gatgtatttc ccaagctatt cagccaattt gtcaaagcat ccaacaccca tgtgatcggt
     5581 tgttcaatca caaataccat caaaagccca ataattaaga tcgataaaaa ggggacgaca
     5641 atcagctgat aaatagcaga aaattctttt tttacgctaa ttttatttgc cagaaaatca
     5701 cagatatacc ctgctagcac gccaccaatc attccgccaa gaaaacctga accacctgta
     5761 gaggccagta atccggcaac aattcctggg gcaaaacctg cttttccagc aatcgatgta
     5821 gcaatccccg cgcctaacgc aggaaccatc atggcaaacg ctgtatctcc tccgatcctt
     5881 gaaaatgcag ctgctaacac attatagtct gctgagttgg gatcagccgc tgtaatccca
     5941 aaggcaaaac tcagtgcgat gatgattccg cctgcgatca caaatggaag catgtaattc
     6001 acaccattca tgaaatgatt atagattgtc ggctttttct catttgcagc tggttctttt
     6061 tcattagaca caacgaatac gccaatatct ctcaatgctt cgtctattac tacttgcggt
     6121 tctttgattg ctcggcttgt agagacacgc tttacttttt tcccggcaaa tcggtccaat
     6181 tcgatttctt tatctactgc aagaatgatt gcatctgcct ctttgatttc ttgttcagat
     6241 aactgatttt ctgtttttac accttgtgtt tcgaattttg cttgatggtt tagatttctt
     6301 gctgttactt ctaatttttc tgccgacata taagtatgag caattccagt cgggcaagca
     6361 gttacaccaa cgattttcat gattattttc ctcctttttc ttcactatcg atactcggac
     6421 aaagactcta ctattttcat tcttgactaa tctgtctgat gatatctgct ggcgtagtgg
     6481 cttcttttat tccctttcta aaatctgcat tcatcaatcg tctggagagt gtgctgagaa
     6541 tctctagatg catatctgct gatttttcag gaactacgat ggcaaaaata tattcaattg
     6601 gcatttcatc tatcccgcta tagtccgtta atttggctgt atgaaaaaaa cacagtgctg
     6661 gttttgtcac atatttgctt tttgcatggg gaatcccaaa tccatcttct attcctgtgg
     6721 agatctcctc ctctcgtttg aatagtgcct taacgacttt ttgaacattt cttaccttat
     6781 ttgttttttt caacacatcc cctattcgct caaataatga tccttttgaa tctatcgatg
     6841 gatcgaaaac aatcagttct tctgcgatca attgatcaaa cctcataaaa aacctctccc
     6901 ccacaaaaaa tgttaacgtt ttcttggaac tgaggtcagt atagcatcgc tagctgtacc
     6961 gttcaatatt atgaatgaac agaggaaaaa gacccatcat ccgcgtgtat aagtcattaa
     7021 aatgaacgat ctagctcaca aacaatcgca agtctataac aaaacaaaaa gccttccttg
     7081 aaaggaaagc ttagcttcta attttaggta aatggttttt tacttcgaaa taacccctct
     7141 tcatttcgtt aatataaggt gtttttatca agtggataat taaaactata tactagtttt
     7201 tcattgtttt gtgcattatg atcctacgac tttttcagca tcaaacgaag ttttaaattg
     7261 gcaaaaaaca taaaaaaaca gactatccct atcagatagt ctgctaaacg ttgagttatc
     7321 agcgttttta ttattccgca gcgtttgcgt ctttgagacc gtattttttg ttgaaacggt
     7381 ccacacgtcc gtctgcttgt gtgaattttt gacgtccagt gtagaatgga tgtgagtcag
     7441 aagttacttc gacacggatg actgggtatg tattaccgtc ttcccattca actgtttctt
     7501 gtgaagtttt tgttgaacct gacaagaatt tgaaaccagt tgttgaatcc atgaatacta
     7561 ctgggtggta atctggatgg atgttttctt tcatgatttt ttcgctcctt tgccctgctc
     7621 catttgctga accagagttg atttgtcgat ttacaacatt aacatcttaa catgtttcag
     7681 tcaaggttgc aattatcttt tcagatttct ggtcggattt tttttaccaa aggagacatc
     7741 ttgaaattct ttgaaaaatt gctgattgtt ttttgtttta cgcaaaaagc ggatcagttg
     7801 ttccgtatat tctaatgaat ctccagtcat attttttcga agtttccaaa tttcttctaa
     7861 ttcttcactt ggcatcaata attcttcttt tcgggtacct gattttttga tatcgatggc
     7921 tgggaagatc cgacgttcag ataattcacg tgaaagttgt aattctagat ttccagttcc
     7981 tttgaattct tcatagatca cgtcatccat acggcttcct gtatcgacta aagcggtcgc
     8041 taaaatcgtc aaactgccac cttcttcgat gtttcttgct gccccgaaaa atttcttagg
     8101 tttatacaat gcggctgggt caatcccgcc actcaatgta cgtccgcttg gtggaacgac
     8161 taagttatag gcccgagcaa gtctagtaat actatccatc aagataacga catctcgttt
     8221 atcttcgact aaacgcattg cgcgttccaa tactaattca gaaactcgag tatggttttg
     8281 cggctgcaga tcaaatgtag aagataccac gtcgcctttc acgcttcgtt ctagatccgt
     8341 tacttcttcc ggtcgttcat caatcagtag aacgattaat tctacttccg gatagttttc
     8401 ggtgatacca tttgcgattt cttttaatac agaagttttc cctgcttttg gcggtgcgac
     8461 gatcaatcca cgctgaccaa aaccgattgg agaaaagata tcgatcattc gggtagatag
     8521 tctttgcggt gttgtttcca gttgaagctg tctttgcgga taaagaggtg ttaaggctgg
     8581 aaaatgcgga cgttcttttg cttcttccgg atcttttcca ttaacgcttt ccacgtgcat
     8641 caaaccataa taacgttccg attctttagg tggacgggct tttcctgcca ctttgtcccc
     8701 atttctcaaa ctgaatcgtc tgatttgtga ggcagaaata tagatatctt ctgcacttgg
     8761 tccataattg attggccgta aaaaaccgta accatcttga ccaacgatat ccaatattcc
     8821 ttccatataa taaaatcctt gtttctctgc ttgtgcacga atcacagcta gagataattc
     8881 ctttttattc atcttactgt aataaggaat tttaaattct ttggcataac tataaacttc
     8941 ttttaacgtt ttgttctcta gttctgccat tgtcaaataa tcgctcattc agctcccact
     9001 tcctctatca tttcaattga tgcccctaag gctgttagtt tctcgataat atgatcataa
     9061 ccgcgtaaga tataatctac attgtaaatc gtcgttgtcc cttctgccaa tagtccagca
     9121 atcactaaac aagcacctgc ccgaagatca gaagccacga cttctgtgcc gtgaagttta
     9181 tttggaccat tcacgatgat catatttcct tccacggtaa tatcagcgcc cattcgtgct
     9241 agttccggca catggttcac acgtttcgca tagatcgtat cgatgatttc tgaagtgcct
     9301 gtagtcatca atagtaacgc agtaagtggc tgctgcagat ctgtggcaaa tcctggataa
     9361 ggataggtca tgatgtttgc gggttttaat tctttcgagg ggtaaacttc gatttcatct
     9421 tcgctgattt tcatatggac accaatttcc tgcaattttg ctataaagct ttccaaatgt
     9481 tcaaagatca catttttcac tttgatacca tttcctactg cagctgctaa tgctagatag
     9541 gttcctgctt cgatgcggtc aggaatcatg aaatgccgac agccatgtaa tgtttcgact
     9601 ccttcgatac ggatcacatc tgtacccgct cctcgaactt ttgcccccat attattcagt
     9661 aaagttgcca catctatgat ttccggttct cgtgcagcgt tttcaattac tgtctgtcct
     9721 tttgctttta cagcagctaa catcacattg atcgttgctc cgactgaaac cacgtccatg
     9781 aaaatccgat tgccacgcaa tcctttattt tctgtccgta aatacatggc accatgttca
     9841 tttgtcactt cagcacctag agcttcaaat cctttgacgt gtaggtcaat cggtctaggg
     9901 cctaaataac agccacctgg caggcctact accgcttcgc caaatttacc aagcagtgtt
     9961 cccataaaat aataagaagc acgcaaacta ttgatttttc cgtgcggcat cggaatcgat
    10021 acgacttctc gaggatcgat ttctagtatg ttgttggaaa aatggacagt tgcgcccatg
    10081 atctctaaaa tttcaattaa tgaatgaaca tcttgtatgt ccggcacacc ttccaagatc
    10141 actggggaat cagctaagat tgcagctgga atcaatgcga ctgcactatt ttttgcaccg
    10201 ctgatcgtta cttcaccagc taaaggtcgg tttcctttga ttacgatttt tttcatctgt
    10261 ttccaccttg tttattaatc ttttttcatg gatatgcttg tattttatca tagataaaaa
    10321 aaagaaacga gcatggtctg ccgttttaag taaaaaagaa tggagagcga cttcaaaagt
    10381 cgttctccat tcctatatat cgtatctgtt atgtggatgc ttacgctttg ttagctgaac
    10441 cgaaccagtc gatatgagat ttagcatctt caacgattgc tgctttacct ggagctaata
    10501 atttacgagg gtcgaatcct ttaccttctt tatctttacc agcttcgata tatttacgag
    10561 tagctgcagc aaatgataat tggaattcag tgttaacatt tacttttgag atacccattg
    10621 agatagcttt ttcgatttga tcttgaggaa tacctgatcc gccatgtaat actaaaggca
    10681 tgtctggacc tacagcatca gcaattgctt gtaagtggtc aaaagctaat cctttccagt
    10741 tttctggata ttgaccatgg atgttaccga taccgcaagc taagtagtcg atacctgtag
    10801 caaccatttg tttgcattct tcaatatcag ctaattcgcc gttaccgatg attccgtctt
    10861 cttctccgcc gattgaacca acttcacatt caactgagat accttttgca tgtgcttttt
    10921 caacaacgtc tttagctaat ttcaagtttt cttcaaatgg tagatgtgaa ccatcgaaca
    10981 ttactgaagt atagcctaat tcgatacatt ccaaagcagc ttcgtaatca ccatggtcta
    11041 agtggatagc aactggaaca gtgatgttca ttgcttcaac taaatcagtg attaaatctt
    11101 tacagatttt gtaaccaccc atgtatttag ctgcacccat tgatgtttgg ataagtactg
    11161 gtgcttgttt tgcttcagcc gcttcaagga ttgcttgagt ccattccaag ttgtttgtgt
    11221 tgaatccgcc gacagcgtaa ccgcctttac gagcagcttt taaaaattca gctcctgata
    11281 ctactggcat aataaaattc ctcctaagtt ttgtaaaact aaatttgtta agacaattct
    11341 taaccaacga ccctatttta ccaaacattt cggtagtttt ctactaaaat caatgaaaat
    11401 gaaaattttc aagaagtttt ttgcaaaaaa taaaatatcc aatagcgctt taatcgaaaa
    11461 aagtcttttg ctttctatct tttttcctac aaataataac aaatcagaaa aagagaaaag
    11521 tgacaagaaa attgcttatc cccttctctt gctacttttc tctttctatc aagttcaaaa
    11581 attattttgt ttcgtgatct gctaatgatg ctccgacaaa tgctttgatt aatttttgcg
    11641 ggcgatttgg acgagaaatc aattctgggt ggaattggca tcccacaaag aattttttct
    11701 ctggcagctc tacgatttct actaaacggt tatctggtga gacacctgaa aagactagcc
    11761 cttgttcttc aaacaattga cgatatttgt tattgaactc atagcggtga cggtgacgtt
    11821 cttgcacaac atcggctcca tcatatgctt cagccgtctt cgttcctttt ttgattttac
    11881 atgggtataa tccaaggcgt aaagttccac ccaaattctc gatgttttcc tgatcagcca
    11941 ttaaatcaat aatattgttt gctgtatctg gattcgtttc agctgatgac gcatcttcta
    12001 atccaacgac gttacgtgca aattcgacac aagccatctg cataccaaga caaataccta
    12061 aaaacggaac gtcattttcg cgagcataac gaatcgcttc gattttccct tctactccgc
    12121 gatcaccaaa tccaccagga accaagattc catctgcgtc tttcaaacgt tcttctacat
    12181 tttctctcgt taattcttgt gctttgatcc aatcgatctc gatgtctgaa ttataggcga
    12241 aaccagaatg tttcaatgct tcaactactg agatatatgc atctggtaac tcgacatatt
    12301 taccgacaag cgcaatccgt acttttttct tcaagttcaa gacacgttct tctaattctt
    12361 tccattctgt catgtctgct tctggtgtat ctagttttaa atggtcgcaa acaatttgat
    12421 ccattccttg tgcttgtaaa actaatggga tggagtaaag tgtatctaca tctggcgact
    12481 cgataactgc ttctggttcg acatcacaaa attgtgccaa tttgtttttc acgctttgag
    12541 aaacaggttg ctctgtacgt accaccaaaa tattcggttg gatacctaaa ccacgtagct
    12601 cttttacact gtgctgcgtt ggtttagttt tcatttctcc agcagctttc aagtaaggaa
    12661 tcaatgttgt atggatatac ataacattat ctgtaccgac atctgctttc atttgacgaa
    12721 gtgcttcaag gaatggcaaa gattctatat cccctactgt tccccctact tctgtaatga
    12781 tcacatctga atcagtcatt ttagctgctc gcatgatttt ttctttgatc tcatttgtaa
    12841 tatgaggaat aacttgaaca gttgctccca agtattcgcc cttacgttct ttacgcaaaa
    12901 cttcagaata gatttttcct gtcgtcacgt tggagtattt gttcaaattg atgtcaataa
    12961 aacgttcgta gtggcccaga tccaaatctg tttcagcacc atcatctgtt acgaatactt
    13021 ctccgtgctg atatgggctc atcgtccccg gatccacgtt gatgtatgga tcaaattttt
    13081 ggatagttac ttttaagccg cggtttttca ataagcgtcc taaagaagct gcaacaatcc
    13141 cttttccgat tgatgaaacg acaccgccgg tcacaaaaat atattttgtc ataagttcaa
    13201 aaactccttt ttaatttatg tgcaggaaaa gtctcagaca ataaacaaag ctccctattc
    13261 ttcaataaga atagggagcc gaaatttctg taaacttctg accctctaaa agggtgccca
    13321 aggtgtataa tacaagctga ctaagggaaa gtcaagtact caaatgaata ctatccgctt
    13381 acaatttttt ctttttcgat atcctttgag aaattatttc ttaagtcctt tatgagtgca
    13441 aaaaaaattt aacccacata ataagcttga tataaacata gctaacgtta aacctaagca
    13501 aatcagaaaa tcgttactga aaaattttaa gtgaaataat agataaaaaa ataaaacaaa
    13561 cattatgata gataatatcc cacctaacaa agaactactt tttcctactt gcagcaacag
    13621 tttatatagc ttatcgtaga aacaatcttt ttctttcacc cttatttacc tcctttacgt
    13681 aaaaaacgta gtccacagtt ctcggatttt tggcatatct tttctattaa ataatatcaa
    13741 aaacaaaaca attccactaa ttttttcaaa gatgaccttt ttttcaacaa aagtgccttg
    13801 tagggattca ttgaaggtaa cgaatcacat aagtaaaaaa taaggagatg cgacaaaagt
    13861 cgtgcctcct tatttctaaa taaacggtgt tctcctgcca actcctcggt cacaagtcac
    13921 aatatccatc aaacatgaga agctgttttt gaaattattt cttgtgattc ggagttaaat
    13981 ggtaggctca caacctctgg ataaacggta ttcaaaagcc agtttctcgg tattaagcca
    14041 aatttatgca aaaatgtaga aagccatttt cacaaaattt ttcttaatac tcgaaactaa
    14101 acgtcttttt caaaacctct tggtaaacgg tgttcaaaag cttgcatctg cggtaataag
    14161 ttcagccaat tcaaaaacaa gaaaagctgt ttttgcttgg ctattcttat tacttgttgc
    14221 agggcagctt tttcacaacc tctccactaa acggtgttca atcagctgac cctcggtatt
    14281 agctcaaaaa acggcaaaat atgaggaact atttttccgt tagtttctct aatactcagg
    14341 tcaaaacgct gatttcacag cctctaattc acggtattcc tgttttaata aagttctaga
    14401 tattgttctc tttcccactc ggaaacagtt tgtctgaatg ccgcccattc gagacgtttt
    14461 gcttcaacaa aatttgagaa gatatgttgt cctaatgcgt caatcattac attatcctta
    14521 cgcaattctt tgatagcatt atggatagtt gaaggtaagt cctggatatg tgcagcttct
    14581 ctttcttcct catccattac ataaatgtta cggtcaacag ctgctggcgg tgtgatctga
    14641 ttacggatac cgtctagtcc tgcttgcaac aatactgcca ttgccaagta tgggtttgca
    14701 gatggatcaa ctgaccgcaa ttccaaacgt gtggataaac cgcgagattc tggtacacgt
    14761 actaatggcg aacgattacg gccactccat gcgacataaa caggcgcttc ataacctgga
    14821 acgagacgtt tataagaatt gactgttgga ttgcaaacgg ctgtgtaggc acgagcatgt
    14881 ttcagcaaac cacctaagaa atgataagct gtttcactta gttccaattc accgtcttta
    14941 tcatagaaaa catttccttc atcattgaac aaagacatat tgcaatgcat accagaacca
    15001 ttgatcccaa ataacggttt cggcatgaaa gtcgcgtgaa gtccatgctt gcgtgcaatc
    15061 gttttgacaa cgagcttgaa ggtttggata ttatcacaag catctaccac atctgcatat
    15121 ttaaagtcaa tttcatgctg tcctggcgct acttcatgat gtgaagcttc cacttcaaag
    15181 cctagacttt ctaattccaa cacgatgtca cggcggcaat tttcacctaa atctgttggt
    15241 gcaaaatcaa aataaccacc tttatcattc aaggtcgttg tgatcccacc gtcttcgtct
    15301 aatttaaata agaagaattc tggttcaggt cctaaattga aagaggtaaa accaaggtcg
    15361 cgcatatctt tcaatgctcg tttcaaattc ccgcgaggat cgccggcaaa tggtgtccca
    15421 tccggattat agatatcaca aattaaacga gcaacttttc catgagcact ttcccacggg
    15481 aagatcatcc aagtggagag atctggatac aaatacatat cgctttcttc aattcgaaca
    15541 aatccttcaa tcgaagaacc gtcaaacatc attttatttt ctaatacttt gtcgatctgg
    15601 ctcactggaa cttctacatt tttgatcgta cctaagatgt ctgtgaacat caatcgtaaa
    15661 aatcgtacgt tttcttcctc aatgattcgt ttgatttctt cacctgttat ctgtttcttt
    15721 accatggtat ccctcttcct tttttaattt attcagtcaa agaattgtct ttgcaatcct
    15781 tgcgactgat aatgacctgt tttgcttaac ccaccttgcg ccaaaatctc atcacgaaaa
    15841 atttgtcgaa cgtcatgatc tgtcaacggc ttcttgtttt ttgcttcttg ttttttcgcc
    15901 tgtgccattt cgtatacacg tttgatccca gccatattca atccctctga aagatagtct
    15961 ttaatctcaa gcaacatatc aatatcattc agcgaataca tacgtcggtt tccttcgctt
    16021 cgttccggat gaactaaatc ttgttcttca taatagcgaa tttgccgagc agacaaatca
    16081 gtcagcttca taactgtacc aattggaaat acggacatag atcggcgcaa ttccttttct
    16141 cccatcagac gtcccctttc gttttataag ttggctaaag tgtaccaatt aaaaaaaata
    16201 ttgtcaactg catatgttag gttttctaac atgagatctc tattttttat aaaataacaa
    16261 aaaaataaga acacaaaaaa ggtatctagt tgattttcgt accagatacc ttttattttt
    16321 tattatactg tactatcctt tattagtgtt tttatt
//
LOCUS       DACDYI010000070        16060 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.070, whole genome shotgun sequence.
ACCESSION   DACDYI010000070 DACDYI010000000
VERSION     DACDYI010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.070"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..317
                     /locus_tag="IWM52_002247"
     CDS             <1..317
                     /locus_tag="IWM52_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9974476.1"
                     /translation="SSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTS
                     SDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKS
                     KR"
     gene            complement(472..1458)
                     /locus_tag="IWM52_002248"
     CDS             complement(472..1458)
                     /locus_tag="IWM52_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP9974477.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1481..2047)
                     /locus_tag="IWM52_002249"
     CDS             complement(1481..2047)
                     /locus_tag="IWM52_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAP9974478.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(2061..2477)
                     /locus_tag="IWM52_002250"
     CDS             complement(2061..2477)
                     /locus_tag="IWM52_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP9974479.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(2934..4868)
                     /locus_tag="IWM52_002251"
     CDS             complement(2934..4868)
                     /locus_tag="IWM52_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9974480.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5170..6105
                     /locus_tag="IWM52_002252"
     CDS             5170..6105
                     /locus_tag="IWM52_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAP9974481.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            6195..7298
                     /locus_tag="IWM52_002253"
     CDS             6195..7298
                     /locus_tag="IWM52_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP9974482.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            7311..8264
                     /gene="menA"
                     /locus_tag="IWM52_002254"
     CDS             7311..8264
                     /gene="menA"
                     /locus_tag="IWM52_002254"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP9974483.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(9078..10424)
                     /gene="gor"
                     /locus_tag="IWM52_002255"
     CDS             complement(9078..10424)
                     /gene="gor"
                     /locus_tag="IWM52_002255"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAP9974484.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10558..11799)
                     /locus_tag="IWM52_002256"
     CDS             complement(10558..11799)
                     /locus_tag="IWM52_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP9974485.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(11817..12740)
                     /locus_tag="IWM52_002257"
     CDS             complement(11817..12740)
                     /locus_tag="IWM52_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP9974486.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(12961..15453)
                     /locus_tag="IWM52_002258"
     CDS             complement(12961..15453)
                     /locus_tag="IWM52_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP9974487.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15460..15927)
                     /locus_tag="IWM52_002259"
     CDS             complement(15460..15927)
                     /locus_tag="IWM52_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAP9974488.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         4711 a         3349 c         2855 g         5145 t
ORIGIN      
        1 cttcaagtga cagtacagat acatcctcta ccacaagtga tagctcagat tcatcaactt
       61 caagcgatag tacagacacg tcgtctacca caagcgatac acgtcatgca agtgtctcct
      121 tctcaaaaac aagttcggat aacaggacga actttaactt aactaataaa acaagcaatg
      181 acaatggaaa caaatcaaaa agagcacttc ctaaaactgg aagtcaatcg aataattgga
      241 taacactcgc aggcgttata ttacttgtaa tcggtttaag aatcactttt atgagttata
      301 agaaatcgaa aaggtaaaaa aatattcaag ataaaaaaga gcaacgtcgc taacggacgt
      361 tgctctttat ttttgctttt tatcataaat acaagaacta caattcaatt tgtcaaaaaa
      421 cacgtagatt tccttctgag aaatttattt acgtatcgaa cctttcccta ttcagtcttt
      481 gcgatccaaa atcagttggg taagggaaaa gagattctct tttgttcgaa gagaggtggc
      541 aggtaatttc tttatttcct tcaaagcctt ttctgtgtaa gaggccgcta acttcctagc
      601 ttcttccacg cctcctgtat gttcaacgat tttgcgtacg gcgtcgacat cttcttctgt
      661 cattgccgct ttcttctcta aataaggtag tagctttggt ttgttttttt gcaaagagta
      721 gatcaacggt aaagaataga ccccttgacg catgtcttct agtacaggtt tgccgatttc
      781 ctggctctct tttgtataat ctaaaatatc atcgataatc tgaaaggcca tcccgataga
      841 aagtccgata tctcctgctt tttttgcgaa gcgctcgctt gttccacttt caaaagcgcc
      901 aacagaacaa cttaattgga acagttcagc tgtcttacct gaaatatttt ccaaatattc
      961 ttttacagaa agttcataat tatagcgatc atccatctgc cctaattcgc cattcaatac
     1021 tttttccata ctgcgagaat tcaattggag acttttcaag gaagtggagt aatcagacaa
     1081 tagtttaaaa cagcagacaa ataaatagtc tcctgcatag acagaaacga cattaccaaa
     1141 ctgactacgg atattaggct gaccacgtcg cagatccgct tcatcaacga tatcatcatg
     1201 gatcagtgtg gccgtatgga gcatttcgat agaagctgcc aaagcgatgg ctttgtctcg
     1261 atcttgctct ggtccaaatt gtgagaacaa tagctgatag gcaggacgca acagctttcc
     1321 gccggcatgg atcgtttctt tgattgcttg ttcgacaggt ttgtttttca ggcggacagt
     1381 ttcttccatc cgttttagtg tttctgtcaa ttctttactt aaagcaggat agttttccca
     1441 cattggatgt agcttcataa aaaaactcct tgtgtgacga ttattttttg gtaaacattg
     1501 ggctccattt ttgtttggtt ccctgctcat aatctttttg gaatcgttga agtgtctgca
     1561 catgtcgtaa aagataattc gctgcgatcc cgattgcaat ccctgataaa atccctaaaa
     1621 ctgaaaggac tggcaagtac aacatgactg tccatgattg tgcaatgaaa gaagccatca
     1681 aaagctgtcc gacattatgc atgaaaccgc cggccgcact aattccgatg atactgacac
     1741 gttttggtcc taattgcttg accaataaca ttccaaaata actaagcaaa gcaccactca
     1801 tactataaag gaatgtggaa atcgttccgc ctagcaaagt agtcaggaat agtcgtagcc
     1861 aaagtagtaa gaagctgtct cgttttttca tggtaaaaat ggcaacgatc gtgattagat
     1921 tggctaagcc tagttttgca ccaggcgcaa aagaaaaagg ataagggatc atattttcca
     1981 ataaaccaat gatcactcct tgtgcaacaa gtagcgaaat ataaatcatt ttttgtaatt
     2041 tactcatggt gtgtgaaggt ctaataaatc agactgtctt cgtcacctcc atctgttgaa
     2101 cggacttcga tcactaattt atgtggcaaa cagacgatgg tttcgccatt tttcgttgcc
     2161 cagcctcgac gtacgcagat ctggtcatcg caatctgctt cttttatccg tactcgatcg
     2221 ccatctatct cgatcaagtt ataatctccg tgttcatctt tgtaagtata tgtgttgctc
     2281 tcttctccag ccatcaaagg gaatgttttg atttctgtgc catccacacg cagtaccgct
     2341 tctttttctg ctgttgcggt gttttgctga agaccaaaaa tcatgactgg taaaaaagaa
     2401 agaacagtca aaagcaaaat aataaaaaca tcccaaggtt tgagcagact tttttcagca
     2461 aattctttaa ggctcatttt tgttctcctt tttccagatt aactcaccca attttagcat
     2521 aaaaacaaga ggttgtgaca cctgtgtctt gctttgagta ataagaacga agaatagaaa
     2581 acagctttcc atgttttcta attctttggg cttattaccg agaagcagca ggtggaacac
     2641 cgtttataac aagaggttgt gacagaagtg ggcaacttca agaaataaga aggaatttac
     2701 gaaaattact tttcaagttt ttgtgaaatt tcggcttatt tttcgaagaa gctacttctg
     2761 gaacaccgtt tataacaaga ggttgtaaca gaggttgtga cacctgtgtc ttgctcatgg
     2821 aggcttatct cgggcatctt attcctttaa ttttacataa aaaggaggct gtgacggttt
     2881 gtctcagccc ctttctattg aaatcttgca ttcaaataac gttctttatc tatttatttc
     2941 ttgccgtact gggaacttgg tctaccgtac caccaattac ctagtgtacg ttgtagccat
     3001 ggaattaccc agcgatccaa cccaatcgca cgtccggaac cgttcatcaa tgcgaaagca
     3061 acaaaaatga accaaatgtt tacccagtaa aacatgcctg ataagcagaa cgtgatcacc
     3121 aatacaatcg ttgtcgcact gcatagccat gtgaataatc cagcaatcaa tgctaatgcc
     3181 aaggcaattt caaagaatac catgaatttt tggaagaaca ttgctacatc attgtttggc
     3241 atgatcactt gcatgatctt tgtgaaccag tctggtgact tctcaatcac agccattggt
     3301 tcttcaccgt atgcataact caaaccaaaa tgtgcagctt gagtcgctgc ttctccggca
     3361 cttgcccctg ctccgctggc agctgtggtt gcatcaccag cagcagaagc accactggca
     3421 acatctgtta cattgtattc ccatgtccaa gggaacacac tgtttccagt gaaccatgat
     3481 ccttcaccaa accaagtact tggtttgaac acttcaccat tcccaatgat ttttttggaa
     3541 gcttcaacga accaaactaa accatagaaa atacgaagtg gcacactcca taagacattg
     3601 ccgtaacgag aagaatgtcc tcgagtcacg ttgcgatcat ctttgatatg gaaaaactca
     3661 tgcattaaat attggaacat ataataacca gaacgaatat caaagaagta tttcaaattc
     3721 acgatatgtt tcattacgat agctaaaaat ccgcttaaat ggatcttatc aaataggtta
     3781 gctacccccc atttggcacc aatagaaacc ataaatcctt gatagtttcc tttaaattta
     3841 tgcttttctc cgcctttgat cgaagccacg atattcgctg cggctgtgtg tcctgtttgt
     3901 tcagcagctt gaacgatctg cggtgtcggt gttcctggga attcttcata aaagactaaa
     3961 tcaccaatga tatagatgtt tttatcttcg tagccttttg cctgcatata ttcattggcg
     4021 atcaaccgct gtccgcgtgc tgattctaat ccaaagtcag cagcgtcaga agttcctttg
     4081 acaccagctg tccaaatcag tgtgtgagtc ggaatgttgc gtccgtcttt caatttgata
     4141 tgatcagcag ccacttctac gattggtgca ttcaataata attccacgtt gtgcttacgc
     4201 aaatagcgtt cagcttttgc agcatcattt cgatccaaca tattcaagat cgtcggcatt
     4261 gcctctacga ccatcagtgt gatttcgttt ggatctaatt tataatcttt ggctaggcga
     4321 tttttccagt cgatcagttc cccaatcatt tcaataccgg taaatccaga accacagaca
     4381 acgaaagtca gcatggcttt acgcagttca tcatctggtt ccaatgcagc ttttgctact
     4441 gtcttctcaa tatgttcccg aatccttaca gaatcttcaa atgaccaaag ggtgaatccg
     4501 tgtgctttga caccaggtgt accaaagtca ttcggctctc cgcccattcc aaggatcagc
     4561 tgatcaaaag gataggaacc taatttggtc ttgacgattt ttcgttcttt atcgataccg
     4621 attacagtgt ctgtgacaag ttgtacattc ttttgacgag caaacaaatg ctgcagatcg
     4681 tattggattg cttctggttc tacacgtccg ccagctactt catgcaactc ggtcatcata
     4741 gtatggtacg aatgacgatc gatcagagta attgctacat cttctttttt cagtttcttt
     4801 gccaagtatt ttgtagctga aacaccggca tatcctgctc ctacaacgac aatattttgc
     4861 tttgccattg atcatccgct ccttatagac tatctatata atgaatatat gataattctt
     4921 ttagtttgat acttattaca caatctccat atattatata gatgttctgt tcttttgtct
     4981 aactcctttt ggattttttt caaattttaa aataaagcgt tttttcacga aatttgctga
     5041 aagaaaacgt ttgaaatatt acatgggtta tttatcaaaa atagttgact tataggctaa
     5101 aaagattaga atgtataatg aatcacaaag aaaaattgtg gactagaaat ggaatggtgg
     5161 ggtattaaaa tgaagaaaaa acgttttgta actggatttg ccgtattagc attttcagct
     5221 ctagtgttag gcgcttgcgg cgcagatgac aacggaagct caaactcttc ttctgaatca
     5281 tctacagcac aatcttcgac tgcaaaaagc acaacagaat catctgcaaa agttgtagct
     5341 ggcggagatt tgcaagacgg cacatataaa ttagaagaaa aaaattattc aaatggttac
     5401 cgtgcagtct ttgaaatggt tgtaaaagac ggcaaaatca ctgaatcaaa atatgataac
     5461 gtcaatgaaa acggcgaatc aaaaacaaaa gatgcagaat acaacaaaaa catggaagca
     5521 aaatctggca caaacccaga aaaatacatt cctgagttga atgaccaatt cttaaaagca
     5581 caaagcgcaa gcggtgtgga agtcgtaact ggagctactc actcatctga aagcttccaa
     5641 aactacgcac aacaattgat ccaagctgct caagctggta acacagatac aatcgaaatc
     5701 gacaatggtg ctgatttgaa agacggcact tacaagcttg aagaaaagaa ctactctaac
     5761 ggctaccgtg tgcaattcga aatgacagtt gctggcggca aagtaactga atcaaacttt
     5821 gattatatcg acaaagacgg taaatcaaaa caagatgata cagaatataa tgaaaatatg
     5881 aaagcaaaaa gcggtacaga accaaaaact tacatcccta ctctgaacga cgaattcgtc
     5941 aaagcaatgg gcgaagaaga tggctcacct gctaatgtag aagttgttac aggtgctaca
     6001 cacagctcac attcattcat catgtacgca caacaattag tgaatgcagc tgaaaaaggc
     6061 gacactcaaa caatcgaagt agataatatc gtaacagaaa aataatcaca taatcatact
     6121 aataaaagtt ggagggtggg acgatagcca gattaggcag tcgtctcacc tttcaaaatg
     6181 aagagaggaa aaagatgcgg aaaaaactac tattttcctt aatcacactg ggcatctctt
     6241 ttatggtgct aactggctgc agcaactcag caaataaaac aaccactgaa agttcagcag
     6301 ctaccaagat attgaaagat ccatattctg atgaacagtt cttgttaggg acttacgttc
     6361 gtatccgtgt gtatgatgaa ggcaaagaat cagcattgaa acctgccttc gatcgagtaa
     6421 aagaacttgg tgataaaatc acgatcaatc aaaaaggttc tgagatcgat gaagtgaatg
     6481 agcaagctgg gatcaaacca gtaaaagttt ctgatgacgt ctacacgtta gtcaagcggg
     6541 cctacgaata tagccaagat tctcaaggcg gattcgatat ggcgattggt gcaatcacac
     6601 aattatggcg aatcggtttt gatgacgcca gaaaaccttc ccaagaagaa atcgatcagg
     6661 cattgaaatt agtggattat cataagatcg aactaaatga caaagaaaaa actgtttatc
     6721 taaaagaaaa aggaatgatc atcgatttag gtgcaatcgc caaaggttac attacggacg
     6781 aagtagtgaa agtcttgaaa aaacaaggag tgaccactgc catcgttgac ttgggcggta
     6841 atgtctatgt cttaggccac agcccaagag gcgaaaacca agactggaca gtcggcatcc
     6901 aagatccgaa catggctcgc ggatctgttt taggttctat taaagaacgg aacaaaacat
     6961 tagttacctc tggaatttac gagcgttatt tagaagtaga tggcaaaaaa taccatcatt
     7021 tgtttgatcc aaaaacgggt tatccgttcg acaatgatat cgcaagtgtc acgatcataa
     7081 cagataaatc aattgatggt gacgggttgt ctacagcagt cttttcaatg ggtgtgaaaa
     7141 aaggactgga atacgtcgaa agcgaattga ataacgggac ggaagcgatc ttcgtgacaa
     7201 aagacgataa agtatatgtg accgatccaa tcaaagacac cttcaaatta tcggaggact
     7261 cccattacac gatgggtgac cgttctgaac taaaataagg agtacgaacc atgacactaa
     7321 aaatcttttt agaagtagtt gaaatacgaa caaaggtggc tagtgtattt ccttttgcgg
     7381 taggggtatt attctctatc gctttttttc atgagataca ttggctaaac acttttttat
     7441 tttttattgg aatgctagtt tttgatttag cgactaccgc tatcaataat tacatggatt
     7501 atgaaaaggc tcattctgaa gtatataagt atgaagaaaa tgtcattggc cgcagaaatc
     7561 tatctcctgt attgattcga aacatgattt tagctatgat tgcatttgtt tttgttatcg
     7621 gcgttattct aacgatcaat acgggttggc ttttactttt gatgggattt atctgttgtt
     7681 ttattggagt attctataca ttcggaccga ttcctttatc tcgaatgcct ttaggggaaa
     7741 ttttcagtgg ggtgactatg ggccttggaa tttttgccat ggtcgtttat gtcaatacat
     7801 tcggttcaga tgtctatgat ctgacagtca acttttcatc tggacaattt gctttggtag
     7861 gaaatctttg ggctatagca gcaatcgtat tagcttcctt acccttggtg tttacgattg
     7921 ccaatatcat gttggctaat aatttgcggg atcttgaccg tgatattgaa aatcatcgct
     7981 atacactagt tttttatatc ggacgtcctg cgggaacagt cttattccag ctattgatgt
     8041 atgcttgcta tttcgtgatt ttaatggggt tgttgagcgg tatctatgaa tggccgatct
     8101 tggtcacctt cttgaccttg ccaattgtga agaagcattt ggatcaattc aagcaaacgt
     8161 taccccagcc tatcagtttc agctattcca tcaaaaatat ggtcttattc aatgccagtt
     8221 atgtattggg gctattacta acaatcatta tgcaagccat ttaacagcat gaagaggttg
     8281 tgaaaaaact gccctgcatc aagtaataag aacagccaaa taaaaatagc tccttatatt
     8341 tttcgtttgg ttgggcttat taccgcagat gcaagctttt gaacaccgtt tatcaggagg
     8401 ttgtgaaaaa gtcgcttagt ttcgagtatt aagaaaaatt ttgtgaaaat ggctttccac
     8461 atttttgcat aaatttggct taataccgag aaactggctt ttgaacaccg tttatcagag
     8521 gttgtaaaat cagtgttttg tcctgagtat tagagaaaca aatggaaaaa tagttcctca
     8581 tattttgccg ttttttgagc taataccgaa ggacagctga ttgaacacta tttgttcgga
     8641 aaaagtgact aactatgaca caattttttt cacagtccct tttttcactt gatttgattc
     8701 gtaccagcga acttgacgag aactttcttg gtttggtaac tatatggtat tcttttaaca
     8761 tcctcgtttg cttcttctaa tttatttata caaagtcaca cttttttaaa catctgttct
     8821 tcgatacatt tctatttctt ttaaacatcg ataccaatat tttgaactta agagtgcata
     8881 ccaaatggat tcatttactc ttcgtcagtt acgaagataa tcaaaatctc caacttccct
     8941 acttatttcg ttttgagaat tatcgaagca atcttacata aataactaaa aaacaaaaaa
     9001 ccagaaaatc agtatactga ttttctggtt ttacatggtt tgttcaataa aaagctgttg
     9061 gaactgttaa tattaaatta tcgcatcgtt acgaattctt ccgctcctgt tggatggatg
     9121 gctactgtat tgtcaaagtc ttctttagtt gcacccatct tgatagccac ggcaaaacct
     9181 tgcagcatct catcgacacc aacaccaata gcatgtaagc cgacgattct ttcttctttt
     9241 ccgacacaga tcaatttcat ttcgcatttt tggcgataat catttaatgc aaagtacatt
     9301 ggggtaaagc gtgaacgata aactttcaat tcattttcgc catatttttc caatgcttct
     9361 tcttctgtca aaccaattgt tgcaattggt ggatgcgtaa agacgactgt cggaacaagt
     9421 ttatagtcaa gataagaatc tttctttcca ttaaataaac gttctgataa acgtctacca
     9481 gctgcgattg caacaggagt cagatctaat ttaccgatca catcaccaac agcaaaaata
     9541 tgttcatccg ttgtattttg gtatttatct actttgataa accctttttt gtccaattcc
     9601 acatttgtat tttccagacc taatttttcg gtattcggta cacgtccacc ggcaaaaagt
     9661 acacaatcac cagttaaggt ttcaccattt tcaaatagca ccgtatattc gtcattgttt
     9721 ttagtaattt ctttagcaat atgatgtgcg taagttttta ttccagcttc ttggtacatt
     9781 tggatcaaat tgtccgaaag cattttatca aacgttctaa gtggtcgttc ttttcgatat
     9841 gcccacatga cttcactacc taaaccacga aatacacctg aaagttcagc cgcaatgtat
     9901 ccagcaccta aaacgattgc tctcttaggt aactctgtta attcaaaaaa tccatttgaa
     9961 tcaattgcgt attctccacc aggaatggtc attttacttg cacggcctcc agtagcgatc
    10021 aagattttag gagcacgata ggtttcaccg tttacttcga tcgtttgact atcgataaaa
    10081 gtagcgtaac cttttatagc ctcgacatga ttgctatcta atcctctttg ataagcaccg
    10141 tgtagaaaat caatatattt ttctcgtctt tctactagac cggcgaaatc aacatttttt
    10201 aaatctgctt ggataccgta acttgaggca tctcgttcaa ttgtttccaa aatcgtactt
    10261 gcttgccaca taactttttt aggtacacaa ccgacgttga cacatgttcc gcccaattca
    10321 ttcgcttcaa tcagcaaaac ttttgcgcca tgcatccctg ctctgtttgc tgaagcaata
    10381 ccgccagagc cgccaccaat tacgatgtaa tcatatattt gcattttgtt cctccttatt
    10441 gaagagatca atcaaaacct ttatttaacg atcctttttc tacagaaaac aagtgtaaca
    10501 cttttcttga attttgtatg cgaagatgct taaaacaggg atatatcagc tttttcctta
    10561 tttacgtttt cggatcatat ctccaggttt caccggctgt gcgactggca tagttaaaat
    10621 catcattgga tttggtgctc ggtcaatgga ttcgccttct tcattatgca taacttcaac
    10681 ggtttgactg aaatgactga atcctggacc ataaaattcg atttcatctc ctacagaaaa
    10741 caagtttctc tgacgtatcg tagcaatctg tgttgcctca tcataagata atacttcacc
    10801 gacaaattta tatactggga ttttacgacg ttcaccgaat aattgttcgt tttcagttgg
    10861 tgtattgtag taaaaacctg tagctaattc tcgttgtgct actttccata actcgtcgat
    10921 ccattcttgc ttgcagacat agttttcagg atcttccata taactgtcta cagccgcttt
    10981 atagacattt gacaccgtag atacataatg gatcgatttc attcttcctt cgatcttgaa
    11041 gctgtctacg ccgttttcaa tcaattctgg aatatgttcg atcattgaca tatccactgc
    11101 actcatcgag aattcttctt ccacttcgcc tgtatcagtt aaactgtttc gttcttgacc
    11161 aaaaggcata tcgaacaatc catacttcca gcgacaagat tgagaacatc cgccacggtt
    11221 tgcatcccgc atcgacatat ggttggacaa tgtgcatcga ccagaataag agatacacat
    11281 ggcaccatgg ataaatgctt cgatttcggt atccgtattt ttacgaatct ctgccacttc
    11341 ctccattgaa acttcacgag ctaaaacgac acgttccaac ccttcttctt tccagaattc
    11401 caatgtctca aaattagttg ctgaagcttg agtggataaa tggatcggca gacctggggc
    11461 ttctgtcgca caaatttcga tcaaagctgg atcagaaaca atcactgcag aaataccgac
    11521 atcacgcaaa ctgcggaaaa attcccctgc tccatcttca tttccttcat gggtgaccat
    11581 attagcagca acgtatacct tcgcatcatg ttcttttgca aaagcaactc cttcagccat
    11641 ttcttcataa gtaaaattac ctgcacggct tcttaaacca tatgcattcc cgccaatata
    11701 tactgcatct gctccataat ggatcgcggt ttttaacttt tccagtgttc ctgctggcgc
    11761 taggatttct ggtcgtttca attttctttt tactgtcatt tctttcatca tactccttat
    11821 ttgatagcag aaggatcaat ataaaagaat cctgtatcta attcacggtt ttcaggatgc
    11881 aatttactga tttcgctagt cattttttcc gctttttctt gatcccagct accttctaaa
    11941 atttgttttt ttgcttcggc gaatagtcga gtgatgttta cgaatttttc gcctggagta
    12001 aataatccgt ccaatttcca tgtacagtaa tgatgctgat aaagttcatt caattcattc
    12061 gagagattca gatcattgct ggcaaaaata tgtgtgccat gactatcttc ataaatagaa
    12121 taatgtgtct cttctttttt aggttccgag ataaaaagac ctctttcacg atctttttgt
    12181 tcgtcttgtt tcgtatagtt atagtaattc tgtagcagtg gacgtttgga ttgatggatg
    12241 catgtagcac catacacaag ggtttctaca ggaatatcga gtttttcttc catcgccttc
    12301 atctcttcaa aaggaacttc acgagcaagt actgctccaa ctgcgccttt tttcgcccag
    12361 aaattgattt gacgtgcgct cgttactaat gtttcagcat cataaatata cggtatacgc
    12421 aattcaggat tttttttcat cgtataaata atacctgtat caccaactgt gatcacatct
    12481 acgtttattt cttttaaaaa agccaaatat tcaggtacat ttttcatttt ttcaggatga
    12541 atgatcccat ttacagcaat attcgctttt ttaccatact tgtgtgctaa ttctaccaat
    12601 tgtcgctgtt cttctcgtgt aaatgaagca ggcagacgca atccaaattg ctcttcaccg
    12661 aaatatagtg tatctgcacc tgcagcaagc aactgttctg cttgttcaat agtttctact
    12721 gtggttatga gttcgatcat aattttctcc tcctacaact tcataatagt agcatagctc
    12781 taaaaacttt ccaatacttc ttctatttaa agtcggattt ttgaaaatta tttcacaaaa
    12841 aatcaaaata aagttcgata tattctgtcc aaatataaaa agagattgtg acaaaaatct
    12901 tgtcacaacc tcttaatcaa gtcttactat tataaaaatt cagctacaca ggccaagtaa
    12961 ctatagggaa cctactgttt cctttttagg tccgcgaaca ttcaatgtga tcttaccttt
    13021 tgaagcacct actgttacgt ggtcacctaa acgaatttct ccgccaagca aagcctcgct
    13081 caatcgatct tcaatttctt tttgaagtgc gcgacggatt ggacgtgcac catattcagg
    13141 atcaaatcca gctttcccga tcacatcgat tgcagatggt gtgattttca cttgaatatc
    13201 ttgttctttc aatcgtttga taatcgcttt gctcatgatt tggacgattg tatggatttc
    13261 atctttactc aatgaatgga agacgacagt ctcatcgatt cgattcaaga attcaggtcg
    13321 gaaagctttc tttaactctt ctaagatacg tttttgcatg gctttgtgat ctttagtaac
    13381 atcttggaca ttgaatccga catttttttc ttctcgaatt tgtgtcgctc cgatatttga
    13441 agtcataatc atgattgtat ttctaaagtc cacttttcta cctttagaat ctgttaaatg
    13501 accatcatcc aatacttgca gaagtaaatt gaaaacgtct ggatgtgctt tctccacttc
    13561 atctaagaga atcactgaat atggtttttg acgaattttt tctgtcaact gtccaccttc
    13621 atcataccct acgtatcctg gaggagaccc gattaatcgg cttgtactgt atttttccat
    13681 aaattctgac atatcaacac ggatcagggc atcttcactt ccaaacatga cttctgaaag
    13741 tgctttggcc aattctgttt ttccgacacc agttggtcca aggaacatga atgaaccaat
    13801 tggacgatca ggatctttca atccgcttcg tgcacggcgg atcgcacgtg aaactgcttt
    13861 gacagcctca tcttgaccaa cgacacgttc atgcagaagt ccttccagtt ctaacaagcg
    13921 ttcgctttct tttttctcca actgctgtag agggacacca gtccactccg atacaactgt
    13981 agcaacatct tcttctgtta cacgatcaga ataaccttgc tcctgttttg cttcaattgc
    14041 agaaactttt tgcagctttt tacgtagtgc tttttcttgt ctgcgtaatt gagcagcatt
    14101 ttcaaagtct tgtttttgga tcgctgcttc cttttcttgt actagttcat cgatttctag
    14161 tttgatcaca gttgattttg ttaagtgatc ggtttgatcc aaacggactt tcgctgctga
    14221 ttcatcgatc aaatcaattg ctttatcggg tagttgacga gaagtgatat aacgaacaga
    14281 aagattgact gctgctcgta cagcttcatc tgtgatctcc actccatggt gctcttcata
    14341 tcttgtacgt aatccttgaa gaatcacttc tgcttcttca ggagttggct catctacttg
    14401 aatgcgagca aaacgtcgct ctaatgcaga atctttttcg atatattttt gatattcatc
    14461 tagggtcgtt gctccgatcg tttgtaattc tcctcgagct aacgccggtt tcaagatatt
    14521 cgaagcatca attgctcctt ctgctccacc tgcaccaatt aaggtatgca attcatcaat
    14581 gaataagatt acttgaccat cattatagat ttcgtcgatg acctttttca ttcgatcttc
    14641 aaattcaccg cgatatttcg taccagcaac caaagatccc atatcaagca tcatcaggcg
    14701 cttttcttgc atgtcttctg gcacttcacc taaaatgatt ttttgagcta acccttcagc
    14761 aatggctgtt ttgcctacac caggttcacc caccagtaca gggttattct ttgtccgtcg
    14821 gctaagaatc tggataagcc gtttcacttc tctcgaacga ccaacaactg ggtccagctt
    14881 gttttctttt gctaatttcg tcaaatcacg tgctaatgaa tctagcgtag gtgttccttg
    14941 cggtgtttgc cgtttatctt gtacatttac atttctacga cgattcattc cttttccagc
    15001 agaatcacca gctagcccca ttttttgttt cagaacttga cgggcttttg aaaggctgat
    15061 acctaagttg atcatgattc gagtagccat gatactttca tcttttaata agctcatcaa
    15121 aatatgttct gttccaactt tttgagaacc agttttcttc acttcgctgc tagctaaagc
    15181 aaaaacttgt ttcattcttg gtgaatatgg aagaaagact cctttagggt aagaacggat
    15241 accaccataa ccaatcagat gttcaatttc ttcataaatt tccgtttcat tcaaatccat
    15301 ttcacgtaat acttttccag caattccatt ttgctcgacc actaaggcta gtaaaacatg
    15361 ttctgaacta actgattgat gtttcaaccg ttttgcttct tcttgtgcaa tttctaatac
    15421 tgcttgggct ctttcagtaa aaagttcttc catctaatct cacttcccat cttcataact
    15481 taaacgttcc agaaatgctc ggagaatccg agcacgtgta tgatcttcat attcattgaa
    15541 attcaatgtg ttttttgcaa ttgcactcaa catcaagttt ccttcttttt ttgtaattat
    15601 ttggtcttca taaagttttt gaataatagc aaaagcattt ttctcactga ttgtatcatc
    15661 aaataactga tttatctgct caagtaattg ttttttatct gaaatcctta cttttgcaat
    15721 tcgtatataa ccaccaccac cacgtttgct ttctactaga taaccacgtt ggatggtaaa
    15781 acgggtatta atcacatagt tgatttgtga aggaacacag ttaaagaggt ctgccatttc
    15841 aatccttcga atctcgatca tttcactatt ttccagaatt ttctttatat atgattcaat
    15901 taaatccgag gtattctgat gtgccatctt attcattccc tttgactaat attgacctta
    15961 attataacga atttgataat agaaagaaag aagaacgctt tttgttaaat aaatccttta
    16021 gaatcaacaa aaaaaaatac ggaatatact attccgtatc
//
LOCUS       DACDYI010000071        15992 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.071, whole genome shotgun sequence.
ACCESSION   DACDYI010000071 DACDYI010000000
VERSION     DACDYI010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.071"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            226..894
                     /locus_tag="IWM52_002260"
     CDS             226..894
                     /locus_tag="IWM52_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAP9974489.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1480)
                     /locus_tag="IWM52_002261"
     CDS             complement(1094..1480)
                     /locus_tag="IWM52_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974490.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1452..2834)
                     /locus_tag="IWM52_002262"
     CDS             complement(1452..2834)
                     /locus_tag="IWM52_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP9974491.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2990..4177)
                     /locus_tag="IWM52_002263"
     CDS             complement(2990..4177)
                     /locus_tag="IWM52_002263"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAP9974492.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4184..5215)
                     /locus_tag="IWM52_002264"
     CDS             complement(4184..5215)
                     /locus_tag="IWM52_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP9974493.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5231..6685)
                     /locus_tag="IWM52_002265"
     CDS             complement(5231..6685)
                     /locus_tag="IWM52_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9974494.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            6860..7765
                     /locus_tag="IWM52_002266"
     CDS             6860..7765
                     /locus_tag="IWM52_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9974495.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIEVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7856..8533
                     /locus_tag="IWM52_002267"
     CDS             7856..8533
                     /locus_tag="IWM52_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP9974496.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8814..9710
                     /locus_tag="IWM52_002268"
     CDS             8814..9710
                     /locus_tag="IWM52_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9974497.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9765..11135)
                     /locus_tag="IWM52_002269"
     CDS             complement(9765..11135)
                     /locus_tag="IWM52_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9974498.1"
                     /translation="MEEKKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11373..12119
                     /locus_tag="IWM52_002270"
     CDS             11373..12119
                     /locus_tag="IWM52_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP9974499.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12116..12955
                     /locus_tag="IWM52_002271"
     CDS             12116..12955
                     /locus_tag="IWM52_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP9974500.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12956..13906
                     /locus_tag="IWM52_002272"
     CDS             12956..13906
                     /locus_tag="IWM52_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP9974501.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14047..14799
                     /locus_tag="IWM52_002273"
     CDS             14047..14799
                     /locus_tag="IWM52_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974502.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14818..15969
                     /gene="queG"
                     /locus_tag="IWM52_002274"
     CDS             14818..15969
                     /gene="queG"
                     /locus_tag="IWM52_002274"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAP9974503.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5192 a         3001 c         3220 g         4579 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttctcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt ttgataccgt
     1381 tgactagttc ttccatcgca aaagcagtcc cccattttaa tccaaatggc cgatctcgat
     1441 actcgttata gtcaatgaat ttttttgctg tccgtctcat cttttccacc ttctaatcca
     1501 ctcataccac ctgcatgccc gccaacgaga gaactacgtg cgatggcgcg tcctccttgc
     1561 aagagactat gcgcatggat gattgattga aagccgtatt tttttctaat cgtatcgaca
     1621 acaaaatcaa ctttcgcttc atttatctgc ttttcaggat cttcaaaaag gttaagctgt
     1681 aaacctgtcg cgtatacgag gtcggaacag ttcacgccta cacgccgaat atcctggtag
     1741 tggtaatgct ggtggaaaat ggtcaataaa gcatctgcta gatctctgct agcatttgtc
     1801 gcttcgattt tcatttgctg gtgaaacccg gtcttgccat ttggatcaag atagcctaaa
     1861 gagtatccta cccataagct gactacttgt gtttttgctg cagatcgtct caagcgagtg
     1921 ccgacttggt cggccatttc tttgataacg atctcgattt cttctcgtct tgtataatct
     1981 ctatctagga tttgactatt tccgatactt tttgattttg gtgtatattc ttgtcccaaa
     2041 aaacttcgat cgatgcccca agcatgggca taaagctgag taccgatgac ccctagcttg
     2101 cttttcaagt gataataatc cgcgtgagct aaatcataaa cagaatgtat acccatttgt
     2161 ttcagacgtt ttgctgttcg tttgccgatt ccccaaaagt cagtgatatc tgagattttc
     2221 cataactttt ctggtacatc ttcataacgc cattcagcaa acatttgctt tccgtctttt
     2281 gagccattgt ctaaggctag tttagcaagt agcgggttat cgccaattcc tatagtcgtg
     2341 tagattccgg tctgctcaaa cacatcttta tggatcagtt tagccatgtc gtacgcattt
     2401 gttttaccaa agagtgccat gctatttgtt acatcaagga agctttcatc gacggaatag
     2461 acatgatggt tcgcttcgtc tgcatatttt ttatagatag aattgatttc tctgttcttt
     2521 tccatataat aggccatcct tggggcaaca atatataaat cacttggata ggggaaggga
     2581 agatctctgg cacggctgat attggtgatg ccgtaagctt tttttgctgc aggactggaa
     2641 gccaagatta atccgctacc ccgtgtttct gtcgaatcgg acggatagga catgactact
     2701 aatttagttg taaggggatc aagatccttt tcaacgcact ctacggaggc gtaaaaagat
     2761 ttgcagtcaa tacataaaat atctcttgaa ggttcttttt cataaacaaa taccggattt
     2821 tgattcgccc acataacaat cacctcttca acacaattag aacacacgtt ctctttccgt
     2881 gtcaataaaa aacagcaatt ttcaaatacg agcaaaaggc tcacatttgg aaattgctga
     2941 gaggttattt ttatctattt gtttttagct gagatcgttg atttttttat caaacatgaa
     3001 cagcttctgg atcgatcttt gttgtcccat ttccaatgtg tgcaacataa aattccggag
     3061 catagccgac aactttcatg tattcatcat agacattgtt tttgaaggca ggtatatttt
     3121 cttctttgac aagagcaatc gcacagccac caaatccagc tcctgtcatt cgtgcaccta
     3181 agacaccttc ttgtttttga gcagcatcta ctagtgtatc taattcgatc ccagttactt
     3241 cataatcata gcgaagagag tggtgagaat catttaacag ttgaccaaat gcttctaagt
     3301 tcccagcttc taattcagct tttgccttca aagtccgctg attttcagaa acggcatggc
     3361 gagctctgcg tatcaatgta tcatcgccga tttggtcaat agttgattca aattcttctt
     3421 catctaagtc tcctaaagaa gagataggca aagttttttg caaacgtgcc aaggcttctt
     3481 cgcattcgct acggcgttca ttgtattttg aatctgccaa ttcccgccgc ttattcgtat
     3541 tcatgatgac gattgcataa ccatctagtt tcactggaac catttcgtat ttcagcgtat
     3601 ttgtatctaa taagattgca tgatctagct caccgaagcc gatggcaaac tgatccatga
     3661 tacctgaatt gacaccgatg aattcatttt ctactttttt acctgtttgg actagttcca
     3721 agcgatctgt ttttaaatca aacagatctt ctaatacaac acctactaat aattctaatg
     3781 aagcgctact ggaaagtcct gcaccattag ggattgttcc ttccactaac aactcaaacc
     3841 catgatcgat tgagtgtcca gcttctttta atttaaggac cattcctttt acgtaatttg
     3901 cccagtttgc ttttttatca tattctagct catctaaagt aaaactaatg acaccgatat
     3961 cgtcaaagtt tgtggaataa actaatattt tattatcgtc tcgaggtgct gctacacctt
     4021 gcgttccata agtgatggaa gcggggaaga catggcctcc attgtaatct gtgtgttcac
     4081 caatcaaatt gatgcgtcct ggtgagaagt attgtgtagt ttttttaggt ccgaatgttt
     4141 cggcaaattt tgtgtttaag cttcttttga tttccatgat gagttattcc tttcttgttt
     4201 tgattttgaa ttctgtcact gtttcaaata ctgatccggc ttttaaatga atcgaaccaa
     4261 atgctgagaa gcgctctgca cctggagctg tttgagtctc aaatgtgatg ccaccgtgat
     4321 gtgccagttt tcggttgcgc atttctggtg tttctgtgcc gaaatttgca gtgaaaataa
     4381 ccacactaga agcatctgta gcaataccta cttggattct tttatcgggg ctagtcaagc
     4441 gagcggcttc tttacccaag ccggtttctt ttaagaaaaa tggatgatcg attccgtcaa
     4501 caagattttt ttgatccaaa tcactagcaa aaacggacgc taatttttta ggtatttgaa
     4561 aatcgaaagc acttcctgca gcattttctt ttacccctat aggaatacta tctgttcgca
     4621 aaggagcata agcttcgcta ttgagccaaa gctcatgttg atcgattgac tgagaagcat
     4681 ctcccgtcaa gttgaagtaa acatgatttg tcggattgaa taaagtatct tggtcactgg
     4741 tgccgcgaga ggtcactcgc cagatattat cttttgtcaa agtatagcgg atttccacat
     4801 ccattgtacc tggaaaacca tgttctccgt cgggacttgt agtagtaaaa atgacagaag
     4861 cttcattttc gccattcaaa atgacataag accatttttt taattcaaat ccaggagcac
     4921 caccatgcag attatgtcca gtttttggat ctgtggctaa ttggtaagtt tttccattga
     4981 gagaaaagcg gccattttcg attctccctg cggtgcgacc gatagaagca ccgatatacg
     5041 gatctttttc gatatattct tcagcggtgt caaatcccaa aactaattct cgatcatttg
     5101 attttaaact gacgattcta gcacctaaat cagagataga aagtgctagc ttgtttttgt
     5161 tctccaaggt tatcaagtga taaccttggc caaaatcttt ttctgtaatc ttcattttca
     5221 agatcctttc ttaatgggaa ggaactactg catcgtgaag ttcatcttcc attgtatcag
     5281 ctggagccaa tttgttttcc aattcctcaa cgatttgtgc atgttttttc tctgtcaatg
     5341 taatcttcca agcaaaaata atagcggaga aaatcatcaa agcagcagga gcatagaatg
     5401 cataagcttt gaaagtcgtt acgccatgag cagtgatatc agcagcagta gcattaccag
     5461 tcatcccaga agcgatagcg acaaacccta cgatcccgtt agacaaagca ccagcgattt
     5521 tatctagtaa agggcgaatt gatagagtaa cagcttcgtt acggatacca tttttccatt
     5581 gaccatattc aacagagtct gtgatcgtca tcaaagcgga taagaaaatc aattgttgag
     5641 ggaagtagaa caatactagc ccgatgataa cgattggcaa acttgaacct gcgatcgtaa
     5701 agaaaaagta agaagaaatc atcattgcga tccctccaag gtaaacgtag cgacgagaga
     5761 tgacgcgtgt aagcattggg aatagtggaa cagctactaa accaagtaag gcagcaacta
     5821 gaccaacgat ccaaaattca gttggttggc caagtacata tttgaagaag tagaataata
     5881 cggcagttgt tgctacattg gcaatcgcat aagcgatata agcaagggca agccacatca
     5941 actgatcatt ttgagcaact gctttgaaca catctttgat cccagctttt tcattttgct
     6001 gacggatcgc agtatcgact tcttttgtgc ccaaggcagt gataatcgca gtaccgcctg
     6061 aaataacagc agcaatcaca ccgaaccaga accagcctga agcgccttca atatgtttat
     6121 gagtaacaag ataggtaaag aaagtaacga tagggacaac gactagagta gtaccatttg
     6181 cacctagaga agagccgaaa cgagcaaacg tagcaaattt ttcacgttcc tttgtttccg
     6241 tactcaaagc tggaatcatt gaccaaaaag cgatatcctt aaatgaataa aaactatcca
     6301 ataagatgaa aacgattgca aataatatga taaataaagt cgtattatga gtagctaagc
     6361 caaagaaatt ggtaaagatt gctaccaaga gtacggaact aacaacgccg ccgataacta
     6421 accatggttt gaatttaccg tattttgtac gagtattatc gatgatgcca ccaatcaaag
     6481 gatcaaaaat aatttcaact aagcggatac ctacaactaa gcttgtaact gtagcaatca
     6541 tttttgcatt cgttgcctta tctgttcctg cgaacatctg gcttgtcacg aataccataa
     6601 aataagtgct taatgtaaca tagaatgtgt cgtgtccaaa agcgcctaaa gcgtaggaca
     6661 cacgttggat cattttgctt ttcataatct gtattcctct cctgttcaat atataatact
     6721 aagtaaaccg gttacaacga ataacaaaga tcttttaatg acttaaactt aacaagaaat
     6781 cgcttccttt ttaatatatt tctgtgactt ttatttaact ttttgttgca ttatactaaa
     6841 ggagtaagga gggatagacg tggagcaatc gattttttta aaaaaggaac atcaacaagt
     6901 agaaaaagaa ttgtattttg atttttgtgg tttctctaaa acgatgccgt ttcattcttt
     6961 tggtcctgct attcgcaatg attacatcct gcatgtcgtg atggaaggca aaggatctta
     7021 tcatgtgaaa aatcaacaat tccaattgaa aaaaggtgat ctgttcttga ttcgtccagg
     7081 agactcgacg ttttacttat cagatggaga agatccgtgg atgtattgct ggctttcatt
     7141 tggtgggagt gctgcagaag aaatcattcg gcagtcgctt ttcagagaag accaatacac
     7201 gatggtctct tctgacatac aaaaatatat cgacattatt ttagaatgca tgaaagatac
     7261 ggaagaaacc ttggaaaatg aattacagtt aacagcatta acctaccgtt ttttatcagt
     7321 ccttttaaaa gatggcggaa aggtcagcgg tggtgtacaa aaggttcatt cctctttagc
     7381 tgttgaaaca gtcaaataca tcgaggcaca ttatgctgaa aaattgtctg ttgaggaaat
     7441 cgcccagcat ttagcggtca atcggagcca tttgtcgcga gtattcaaaa atcatatggg
     7501 taccagtatc aaggaatatt tgatcgaggt acggatcaat cgaggggcgt ttcttctttc
     7561 tctgacggat gaatccgtgg aaagtattgc ctatcaagta gggttcaata gtctagtcgt
     7621 atttagccga atgttcaaaa agagtaccgg agaaacggct acgaactatc gtaaacgtat
     7681 gaaaaacgaa aattttaaga atattgcttt ggataccttg aaaaaacagt tggaaaaaca
     7741 aaccgcagtt tcttggacaa cctgattagt ctggttaaaa tgacaagcat cggcaaaaga
     7801 aacttgtggc ggttatgcta cactgtaaag cgaagactaa aaaggaggaa aagggatgcg
     7861 tacacttatc atcattagcc atccaacgat cgaagaatct ttaaatcagc aatttttcaa
     7921 agaagcttct gctggtttac cagctacttg gcatcatttg gaatcaagtt atccagacgg
     7981 aaaaatagat atagagactg aactacagct cttggcccag catgatcgga tcattttcca
     8041 gtttccgttt tactggtata gttctcctgc tcatttaaaa atttggcaag ataatgtctt
     8101 ggaacgagca gcacatgtat taaaagggaa agaactagga atcgtattga caacaggtgt
     8161 ggcggaaaaa gaataccaag ctggaggaaa agaagaatat acgatttccg aatttttgag
     8221 gccgtatcag cgaatcgcaa ataaattcca tatgacctat cttccgccat tcgttttagc
     8281 acaatttatg tatttatccc aggaaaaacg ttgggagaag ctaattgctt atcagcagta
     8341 tctctcattg gaaggcaaac catcgctgac tcagcggatt gactggttta ttcaaagagt
     8401 acaggaaaat cagaaaatgc aggaagaaga atctgaaaaa caagcatata tcgtagaagc
     8461 actgactgag gcaaaagagc agatcgaaga cttgaccttt acactgcaag aaatgaaagg
     8521 aacttcatta tgacaaatga ttggctgatc caacaattag atgaattaag cgaaaaatca
     8581 gaatatcaag accgtgcgct cttatatgaa ataaagaaac tgatggctga gatagacgaa
     8641 cgaacaatgc agctgcaagg agaaatcgac ggacaatctt ggaatcatct caactggtga
     8701 aattttctta taaataacaa agataaaaat gtgcacagcg acaattattt gctacaataa
     8761 gaatatgaag cgtttgataa cgctattata cataaaaatt ggtggtgaaa aaaatgtatg
     8821 atgaaaatga gtggggagaa aatgttcgga tgacccgaag aagacagcaa aaacaggcag
     8881 ctcgtaagcg gatgatcatg attggggcag cagttgccgt gtttttgatc atagcaggag
     8941 gagttgcggg tctatatttt catggaaaaa attcgtcaga agcgaaaagt acgaccataa
     9001 cggaaacaac gacaaagaaa acagagacga aacaaacaag tgagtcatca actaaaacaa
     9061 aagagacaca aacttctgaa gaaaaaacag caaatggttt gccagagcaa cttcaagcaa
     9121 tcgtaaatga ttattcgaaa aagatcgaag aaaagacgcc tgcattgatt gaagaatacc
     9181 agacagaaat tcagggaaat caagagggaa tagctggttt atcagcagtt gctaaccaaa
     9241 aagcacgtga gcttcaagca atctccgacg aagggatcag aaaattacgt gcagcgtatc
     9301 aggcagcaga aaataaagac ggtgtagact tagatacttt gatcaatcaa ttatcggcta
     9361 attatacgaa tcatgtcgct aagatttccg atatttattt gcagactagc gcctctcttc
     9421 aagcagagtc aacaagcaca caagacacaa catcttctga ttctgagaca gcagagtcgg
     9481 tagaagcttc tgaaatgcaa gctcgaacaa cacaagacac aacagattcc tctgaaacta
     9541 ataccaacag cgcgacttca caggctgcga caaccgtagt tttggaagga gaaggtccaa
     9601 atcaaatagc cgaacgaact ggcgtaccag tagaaacgat cttgtcgctg aatgggatga
     9661 gcatggagaa ttatttcctg actcccggtg agacgctaaa attgaattga taaaagcaat
     9721 aaaaaagaac gaagcttcca ctaaggtgaa gcttcgttct ttttttattt tccggttctt
     9781 ttttcgcgta caaagtaatg gatcgtcatg taaagcaaga aaacgagtcc aataatcaat
     9841 gagattctcg tttcaggatt gataaacatg aaaatcaata cgatagctaa aaaagcaagt
     9901 gtcacataat tcgtataagg agatagtggc attttaaaag gatgatcatg cattttatct
     9961 gcgtgcttct ttttgaagcg aatctcgctg atcaaaatca ctacccaagg aaccatacca
    10021 ggcaaaatac ttgagctgta tacgaaaacg aaaatatttt ctgactggtt gatgaacatt
    10081 ggtagaagat aatttaagat cagaccaatt aagattccac ctgagattgc tacgacagga
    10141 taaaaaggcg caccgtgacg agatagtttc agaaattttt tagacatctt tccatttatg
    10201 gcaagtgtat agaccatacg acttgcgctg aaaatccctg aattgcaacc tgataatgcc
    10261 gctgtaatca cgacaaaatt aatcagtcct gcagcaaatg taatgccgac tttagaaaaa
    10321 gtttgtacaa aaggtgaacc aatctcactc aattgattcc acggatagat actcacaatg
    10381 ataaaaatag caccaatata aaaaatcaag attcgaccga tagtggactg gacagcttca
    10441 atgatcgtct ttttaggatt ctctgcttca ccagcagtca ttccgatcag ttcgattcct
    10501 tgataggaag caacaacgat cgataatgca aagaaaaatc ctttgacgcc tccagtgaag
    10561 aatccaccat ttttccaaag atttgaaatc cctactgcct gaccatgatt accaaatcca
    10621 aataagatta ccccaagacc tgcaatgatc atcaaaatga tcgttatgac tttgatgatc
    10681 gaaaaccaaa attctaattc gccaaacgcc ttgacagaaa taagatttgc cagcataaga
    10741 aacaagacga caatgattcc gggaataatt gccggcaaat caggccacca ataattcata
    10801 taagatccta cagcaatcac ttcacttatt cctacgacaa tatattgaaa aatattactc
    10861 caagcggtca agtagccagc cacaggatgg atgtactcgc tcgcgtaatt tgcaaaagaa
    10921 ccagtcgaag gatcaacgta aagcatttcg cctaaagcac gcataatcaa atacaaaaag
    10981 aatcctgcaa tcgcataggc tcccatcact gagggccctg tccaacgtat ggttgaggag
    11041 gcacccataa acagaccgac accgatcgtt ccacctagtg cgatcatttg gatatgacgg
    11101 gcactaagac ttcgagttaa ctttttttct tccatacaaa aactcctttc gttgttgagt
    11161 caacaagaaa aaataatcaa acgtttcaac acatgttttt cattattagg ttagccttat
    11221 tgaactcttg atgtgattct acagaaaatt ttcataaaat gcgaggaaaa ttcgaaaaaa
    11281 actttttcat cattctactt gaaaatgctt tccattttag ttaaactata tttaaataaa
    11341 aagttaggta aacctaaaaa ggagtgtaag atttggaacg tacggccatt gtaattcaag
    11401 acctttcagc cgcttatcaa gggaaaactg ttttgaaaac tatcaacttg acgataaatc
    11461 cacaaaaaat taccggtatt atcggaccaa acggtgcagg aaaatcaaca tttatgaaat
    11521 ctttgctaga gttgatacca gctactactg gtaaagtcac cttttctgga cagccggtaa
    11581 actcagtccg taaaaaaata gcctacgtgg aacagcgaag tgaattggat ctttcctttc
    11641 caattgatgt attaggcgtt gtacttttag gaacatatcc atctttacga attggacaaa
    11701 gacctgggaa aaaagaaaaa gaacgtgcaa gacaagcttt gaaaaaagta gggatggaag
    11761 aatatgccaa aagacagatc agcgaactat cgggtggaca gcttcagaga gtttttattg
    11821 caagagctct agcccaagga gcagaatgga tctttttaga tgaaccattc gtagggattg
    11881 atgcgttaag tgaacgaaag atctttgaca tcttgcagga attgaagaat tcaggaaaaa
    11941 cgattttgat cgtccatcat gatcttcata aagtagaaga atatttcgat gaggttattc
    12001 ttttaaataa acagctgatc gcttccggtc cagtacaaga agtctttaca tcgaagaacc
    12061 ttcaattggc ctatggtgag atcatcagac atttagtaaa aggaggagag aaaaaatgat
    12121 ccaatccttt attgatggat taatgaatta tcaattcctg caatacgctt tggtcacttc
    12181 catgcttgta ggacttgctt ctggagtcat cggttctttt attattttac gagggatgtc
    12241 tttgatggga gacgctatct ctcacgcagt tcttccaggt gtagccattt cttatatgtt
    12301 tggttttagt tacatctttg gagccactgc ttttgggatg ctaacagcag cagtgattgg
    12361 tttcgtgact cagcggagcc gattgaaaaa tgatacagcg attggaattg tctttagctc
    12421 cttttttgca ttagggatta tcttgatttc atatgcccag agtgcgacag atttgtatca
    12481 tattttattt gggaatgtct tagctgttag ggaaagcgat ttgttgctga cagcactcgt
    12541 tagtggaatc gtcttgatat ttgtcttttt cttttataaa gaattgaaga tcacatcatt
    12601 cgatccgaca atggcaaaag cttatgggct gaacacatcg ctgatccatt atcttttgat
    12661 gttctttttg acattagtgg ctgtagtcag tttacagaca gtaggaacaa tcttggtgat
    12721 tgccatgttg atcacaccag ccgccacggc ttacttgcta acgaaccatt tactgaaaat
    12781 gatcattaca gctgcaggaa tcggtatgct aagtgcagtt gtcggtgtgt ttttcagtta
    12841 tagttacaat tggccatcag gagctacgat cgtgttagca tgtaccgcat tttttatcct
    12901 tgctttttta ttttctccaa caaaaggaat tttatttcag aaaggaagca gatagatgaa
    12961 aacaaaaaaa tcactctttt taatcttggc agttagtttc ttggtattag ccggttgcgg
    13021 aaagcaggct tctgaccaag cggatgaagg aagtaaagaa aagttgtcag tcgtggctac
    13081 caattcgatc ttggcggaca tggcaaaaga ggtcggaaca gatcaaatag atatccacag
    13141 tatcgtacca gtcggaacag atccgcatga atatgaagta ctaccagaag acatcaaaaa
    13201 ggcaagtgat gcagatgtta ttttatacaa cggtttgaat cttgaaacag gtaacagctg
    13261 gttcgataac ttgatggaaa cggctaaaaa agaagaagga aaagattatt ttgcagttag
    13321 caaaaatgta gaacctcttt atttaactag cggtgaagaa catacaaaag cagatcccca
    13381 tgcttggcta gacctatcta acggaataaa atatgtggag gaaatcgcac gtatattctc
    13441 tgaaaaagat gcagaaaatg cgacactcta taaaaaaaat gcagaagcat atgtggaaaa
    13501 actaaaagaa ttagatacac aagcgaagga aagttttgct tctatcgaag agaacaaaaa
    13561 attattagta acaagtgaag gggcattcaa gtatttttca cgagcatatg atttgccagc
    13621 agcttatata tgggagatca atacagaaag tcaaggtacg cctgatcaaa tgaaagcgat
    13681 cattgatcag ataagagcaa aagaagtacc ggttttattc gtggaaacca gtgtagactc
    13741 aagaagcatg gaacgggtag caaaagaaac gggtttgaaa atctatgata aactgttcac
    13801 tgattccata gcaaaagaag gagaacaagg agattcttac tatcagatga tgaaatggaa
    13861 tattgaaacg atccatgaag gtttgtcaca aacaaaagaa agctgaaaag tgcaaggtct
    13921 agcttgagga gcagaccttg cattttttat aagagatatg aaaaaaaatt atgctgtttt
    13981 tcgtcgtaca gatacctttt ttgaagaaat tccgttatag ttagagtaag gaaggaaggt
    14041 gtggctatga attttacacg taaagataaa tatacgatta aagaagaagt ctttcgattg
    14101 atcagccagc aggaaaatac cgacaaggat caagaccttc aatttcttga agcagaatta
    14161 gctaaattat tcatagcaaa tcaaaatgag acaacagaac cagatgaaga gctgtacata
    14221 ggcaaatggt tgtttgatga atttcagcct ttagatgaaa ataccggtat cggttatttt
    14281 gtgttccttt ctccagtcgg cttcacacct ggcgtattag ttaaacaaaa cgacgaaata
    14341 accgtcaaag catggcctgc attattttca gtcatcaaag cagaaggagt aatcaaaaag
    14401 atccagtcgg cctattataa aagtcaattt attgccaaca gcaaattaat gaatatgttt
    14461 tataatcgtc tagtagaaat tgctaggcct gggaaaatcc cgcaaacatt cacgtacaca
    14521 cagcaaaaaa atctctatga agaagtattg tattcccgcg atctgtctga gttttcgcca
    14581 tgggaagagg taaggctcca tgaactgtta agtacaaaac cagtttcatt gaattttttt
    14641 aatgcattag ataacaaaag aaaattaatt tcaacaaatt attgggaagt atttgctgca
    14701 agaaaaaaat ggatggaaga aaagaagatt gccgatcagc agatccagtt tgtccagctg
    14761 aaaaaagaaa aactatatta tgggttatac agccgttaac agcaagcgag gaagaccatg
    14821 acattaaaag aagaaatcat acaagaaagc aaacgattag gaattgacaa aatcgggttc
    14881 accacggcgg aaccttttga ttcattaaaa gaatcgttgg aagaacaaaa agcagctggc
    14941 catacttctg gctttgaaca tccaaatatc gatgagcgtc tttatccaga aatgacattt
    15001 gatcagccga aatcgatcat ttccattgca cttgcgtatc ctactaggat ccatgaagaa
    15061 gtgcctaggg atgaaaaacg cgggcagttt gctcgtgctt cttgggggat cgattaccat
    15121 gatatactca gagaccgttt gaaccgattg atcgaattca tccaaacaag agcagaaaaa
    15181 tggcaaaaag aagaggaatg gcgtctagct ccgcaagtag atactggaga actgatcgat
    15241 gtcgctgtag cacagcgagc tggtttagga tttattggac gaaacggatt gttgatcact
    15301 gaagagttcg gttcatttgt ctatcttggt gagatcgtta ctaatattgt atttgagcct
    15361 gataaaccag gagaatttgg ttgcggtgac tgtatgcgct gcgtgactgc ttgtccgaca
    15421 aaagcattgc ttggtgacgg aagaatgaat gcccagcgat gtttgtctta tcaaactcag
    15481 acaaaaggga tgatgccgga agaatatcgt aaaaaaatgc gaaatgtcat ttatggatgt
    15541 gatatctgcc agttagtctg cccttacaat caaggaaaag attttcattt tcatgaagag
    15601 atggaaccaa aagtagacga agtatatcca aaattaaagc caatgctttc gatgtctaat
    15661 aaagacttca aacagcaatt tgggcattta gctggttcat ggcgtgggaa gaaacctttg
    15721 cagagaaacg cattgattgc tttagctaat ctaggtgatc gaacggcatt gcctgagatt
    15781 tcagaatgtt taacagatgt ccgccctgtc atcagaggaa cagcagcttg ggctattgga
    15841 cgtctaggaa caaaagaacc ggaaaaatgg cttgatgtat tagatgaaat attagctaaa
    15901 gaaacagaag aagtcgttat aaacgaattg gaacatgccc agaaattatt gcggagaaag
    15961 ttaaaataaa aaaatagtta acattattca aa
//
LOCUS       DACDYI010000072        15821 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.072, whole genome shotgun sequence.
ACCESSION   DACDYI010000072 DACDYI010000000
VERSION     DACDYI010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.072"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(58..360)
                     /locus_tag="IWM52_002275"
     CDS             complement(58..360)
                     /locus_tag="IWM52_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974504.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(580..946)
                     /gene="ssrA"
                     /locus_tag="IWM52_002276"
     tmRNA           complement(580..946)
                     /gene="ssrA"
                     /locus_tag="IWM52_002276"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1117..1890)
                     /locus_tag="IWM52_002277"
     CDS             complement(1117..1890)
                     /locus_tag="IWM52_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9974505.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2034..2384
                     /locus_tag="IWM52_002278"
     CDS             2034..2384
                     /locus_tag="IWM52_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP9974506.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2365..3081
                     /locus_tag="IWM52_002279"
     CDS             2365..3081
                     /locus_tag="IWM52_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9974507.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3081..4529
                     /locus_tag="IWM52_002280"
     CDS             3081..4529
                     /locus_tag="IWM52_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9974508.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4599..5108)
                     /locus_tag="IWM52_002281"
     CDS             complement(4599..5108)
                     /locus_tag="IWM52_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974509.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5098..5823)
                     /locus_tag="IWM52_002282"
     CDS             complement(5098..5823)
                     /locus_tag="IWM52_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974510.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(6046..8166)
                     /locus_tag="IWM52_002283"
     CDS             complement(6046..8166)
                     /locus_tag="IWM52_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP9974511.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            8396..9574
                     /locus_tag="IWM52_002284"
     CDS             8396..9574
                     /locus_tag="IWM52_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAP9974512.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9590..10348
                     /gene="aroD"
                     /locus_tag="IWM52_002285"
     CDS             9590..10348
                     /gene="aroD"
                     /locus_tag="IWM52_002285"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAP9974513.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            10371..10856
                     /gene="ybaK"
                     /locus_tag="IWM52_002286"
     CDS             10371..10856
                     /gene="ybaK"
                     /locus_tag="IWM52_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAP9974514.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(10927..12180)
                     /locus_tag="IWM52_002287"
     CDS             complement(10927..12180)
                     /locus_tag="IWM52_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9974515.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            12297..13646
                     /gene="gdhA"
                     /locus_tag="IWM52_002288"
     CDS             12297..13646
                     /gene="gdhA"
                     /locus_tag="IWM52_002288"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAP9974516.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            13758..15104
                     /locus_tag="IWM52_002289"
     CDS             13758..15104
                     /locus_tag="IWM52_002289"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAP9974517.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(15412..15798)
                     /locus_tag="IWM52_002290"
     CDS             complement(15412..15798)
                     /locus_tag="IWM52_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9974518.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         5171 a         2830 c         3180 g         4640 t
ORIGIN      
        1 cttttataag tatggtgata aaatccgttc cgggctacct tttttaggta gcctacttta
       61 atctttatac ctttctggat caacgaaagt atacttcaaa tagtcataac gccgatgatc
      121 gctccgtgcg tctggcacat cagtcacgat atcaaacaaa aaatacacat ctttcttcat
      181 tctggttttt gttgccggaa ttttaaagta gttcttatta gagtaataga gattgattaa
      241 taagctatct tcgattgcta aaaagaaaac ttctgaatcc cacaccttat aaaaatcttt
      301 gacaaatcta ttcgaaggat caaatttaaa ccataattgt gtttttcctt ccatcaacat
      361 aatgttcacc tcaaaaagag tatacgaact aatgttcttt ttgtaaagta ggaatatcat
      421 tgataatatg gatggaacat atatattaaa aagtgttctt tttgaatgct attttgaatg
      481 ccattttcat tcatttaacg aaaatctgcg aaaataaaaa atgctcaaac ccttataaaa
      541 taaggattta agcatctagc gaaatctgct gaaatctcac aatggagacg gcgggagtcg
      601 aacccgcgtc caaacacatc gccacttaaa aatctacgtt catagtcaac tcatttaagg
      661 attcacttta cagcttgccg agtgacaggc attctgtatc gctagtctga tgttctcttt
      721 ttcaacttac agacggaaag ttgaaacgta tcccactaaa gttaggaccc ttactcgagc
      781 acatgggcga tgccgagagg atctacgcta actgttttta ggcagctaaa gcgtaagagt
      841 tgttttcgtt tttagcagtt atatttaact gtaacgtttt tacgtagacg taacctacga
      901 aacgcaattc aagctcgact gtgcctgtcg aatccgtaac gtccccgtta gctaacaagg
      961 gttacgtgta tttttcatac aaaattagta tagcatatat tgggataaat tcaataaaaa
     1021 agcgaatcat atcccattat ttatataata actgcccctt tcttaagaaa aaataagcga
     1081 ttttcttaag aaaagaggta gcttcaaaaa aagatcttaa atcagatcaa aatacttcaa
     1141 tgcgtggaca attccgcctt ctgtattttt ctttgtgata aaggtagaaa gttcttttaa
     1201 tccgtcatag gcgttgccca tagcgatttt gttgtcgcaa gcttccaaca aggcaaaatc
     1261 attcggacca tcgccaaatc caaaagtagg aactttttcc agctgaagtt ctttttgcag
     1321 acgtttgaca cctgcccctt ttgagattcc tttcttcact gtatcgatcg agtaaggacc
     1381 attccgataa aaagttagct caggaaaatg ttcgtgatag tattcgtcac ctgattgaga
     1441 taagatcagc aacatattta ctgtgttttc tttaaagcct aaaggatcaa tttccggaac
     1501 atctgaatga atgaagtcgt aagcattgat cattgtttgg ctatgaccgg tacaccagat
     1561 tttctgatcg ttatagtaag aaagttcatg accgttcaac tgtacatgat catacatttt
     1621 ttgacattct tctgaagaaa atgtttgaga atagatttct tcgccgtcta ctcgaatgaa
     1681 agaaccattc atggtgaccg cagaagtaat gcccgcatct tccatgatag agtggatttc
     1741 tgcttctgta cgtcctgtag cgatgacggg gagaacattg ttttctttta atgcgttcat
     1801 agcttttgcc acttcgggtg tgatttttga tttcgcatct agtaatgttc catcaagatc
     1861 aaagaaagtg attgcacgat aatttgacac aaataaaact ccttttttat tggaataagt
     1921 ttatcttaac aaagaatgaa caaaagacaa acctttcgtg aagaattcct gaacatattg
     1981 gttcagtcaa caatttagag tatactataa gaagagaatt taggagggaa agaatgattt
     2041 ttttcagtat tggtatctta ttcttaataa ttggcagtct ttttcggatt ctgccctcaa
     2101 aaggaaatct gcctttttat gggtatcatt cgcctcttgc cgcaaaaaca gacgctcatt
     2161 ggcggctggc tcaaaagaca agcggaaact ggttttttct tatgggctta ctgatggcat
     2221 tgatcggcta ttatttaaag acatctgggc atacgaatta ttttctgatt gaaatgcttc
     2281 tgctagtatt tccaattatg cctattttta ttatgacaga aaagaaatta cagaaatatg
     2341 acctggaaac tggagggaac gataatgaat attttaatga ttgaagataa tcaatctgtt
     2401 tctgaaatga tgcagatgtt tttcttaaat gaaggttggg aagcaacttt caagtatgat
     2461 ggaaaagaag ggctcgatgc ctttttagaa aacccacaac attgggatat gattacacta
     2521 gacctgaatt tgcccacact ggacggtatg ctagtaggaa gagagatccg caaagtttca
     2581 agtactgtgc cgattatcat gctgacagct agagattctg aaagcgatca ggtgatcggc
     2641 ttagagatgg gggcagatga ttatgtcaca aaacctttca gtcctctgac gcttattgct
     2701 cgaatgaaag ctttgcatcg ccgtagtgag ttggcagaag caagagaaga agcagtagtt
     2761 tcagatgatc attttgatat cgagactagt catttcaaga tgaatacgaa aacacgagaa
     2821 gcgtatctgg acaaccggcc aattgaagga ctgacgccta aagagttcga tctattgttt
     2881 actttagcaa aaaaaccacg tcaagtattc tctcgggagc agttgcttga attagtctgg
     2941 gattatcagt actttggtga tgaacggaca gtggacgccc atatcaagaa attacgacaa
     3001 aaaatcgaaa aggtcggacc acaagtcatc caaactgttt ggggagtcgg ctacaaattc
     3061 gatgattctg gtgttgccta atgcgttatt tgtatcagca actcttagct ttctgcggaa
     3121 tggtcgcgtt gatcttattg atcgttggag tttcttttac acaactgaca aaacaaacga
     3181 ttgaagaaaa caattaccag caactgtttg gatatgctga atcagttgaa aagacaacgc
     3241 aaacctatgc cgatacgctc ccgcagttta gtcaagatca ggcgtttcaa aatgcattgc
     3301 tgctgacaga gcaagtactg acagaacaaa atgtgaactt tatatttatc gataaaaacg
     3361 aacgtgtcat ttatccgact aatacagctt tactgaattt taccttaaca gctgatcaat
     3421 ggaaaaatct gaggaatgga cgtcaagaaa aattcacatc gaataaaaat attttaggac
     3481 aagcacaggc gacctcttac gcacttgtgc catttaattt gaatcatgaa ttctatggag
     3541 cacttgtagt tagtcaacct gcgcgaaata tcgataacag cgttcgtcca attacactga
     3601 acctgttcaa aggatttatt ttttccagta tcgttgcagt tattgccagc tatgcttttg
     3661 cagcattcca agttaagcgt atcaatcggt taaggaatgc gacgaaagaa gtgacgaatg
     3721 gtaattttga tgtacagctc cccgtacatg acaaagatga atttgatgag ttagcagatg
     3781 atttcaataa aatgacgaat tcattgaaag aatctcaagc agaaattgaa gaacaagaaa
     3841 atcgtcgaag acaatttatg gcagatgctt ctcacgagat gagaacacct cttacgacaa
     3901 tcaacggact tctggaaggg ttagaataca atgcaatccc tgaaaatcag cgagaaaacg
     3961 cgattaaatt gatgaaaaat gaaacagagc gtctgatccg tttggtgaat gaaaacttgg
     4021 attatgaaaa aatccgtacg aatcaaatca gtatggtcat taaaaaattc aatggaacag
     4081 aaacattgaa aaatattgtt gcacagttag aagctaaagc agaagcagca ggagatacat
     4141 tgacattgaa agccgttgat gatattgatg tttacgctga ttatgaccga tttgtccaaa
     4201 tcatggtcaa tattattcaa aacgcgatac agtttactga aaacggccaa atcacgatta
     4261 ctttagaaaa aggctatctt gaaacgatca tcacgattga agatacggga attggaatgt
     4321 ctgaacaaca aatgaaaagt atctgggatc gttattataa agtagatcct tcaagaaaaa
     4381 atacaaaata tggtgaatca ggattaggtt taccaatcgt gcagcaactt gtacgcttgc
     4441 ataaaggaaa attagaagta gaaagcgaac taggaaaagg aacaaaattc gctgttcgtt
     4501 tgcctgacgt agaaatcaaa aaagagtaaa aaacaagcgg tataaaagat catgaatcga
     4561 tcttctatac cgcttgtttt tactataaaa tgattgattt agcccgcggg ctctaaacga
     4621 tcaatataag gactttgagc aatagtagca tcatgagtaa agagtaagat accttgattt
     4681 tcttcatgag ctaaataaga aagcaagtgg agtagctctt ggatctccaa tacactaagt
     4741 tcatcaaatg tatcttcaat aacaatattg tttcgcttgg aaagcaaggc atgaagtaat
     4801 tgcagcttta ctttttcaaa agacgtcaac tcagctgccg cacaattaag caaggaagga
     4861 gagatatcaa gtttttcttg ataggccaaa cgatcttgtt tgatttcttt ttccggtaca
     4921 tccaacagga catggtcttt aagattcaga taaggaaaca tcggccaatt tttttggatg
     4981 atcgcaaaat tatccagttt tgcaacaaga acctcttttt tcaagtgctc caatatttta
     5041 tgctgttttt cgagaggaca caaaataacg gtcaagtgtt cagcatgaat caaatattca
     5101 ggtttcatta cgtccaggcc tcctactttg caaatgatgg ataaaaaaag tgaaagggaa
     5161 tagactaaga atgagacagc atccactgag cccaataaga gtttgggcaa atcccttaag
     5221 tatatctgtg aaaaaaagat cattttggaa atcgatggta aaaaaagtct gttgatccat
     5281 cggaattgta agtccgctta ccgaagtcaa gatatcacgg atatctggca gttcagtagt
     5341 ctttaagaca agaaaattga tcctttgaag aagcgcttca taaactgact ggaagcttac
     5401 gatccacaat aaaaccaaga tacaacaaag aaagagagga aagaacagct gccagaaaat
     5461 aaaaaggaat aatttggttt tagaaagacc agtcaggtgc caattgatca attcctgctt
     5521 gttttgcctg actgaatagt aaaagaaaaa gataaaaagg aaggcaaata gaaaaaataa
     5581 actgaatatg ccttttatat aaaatcggct agctaattct aaagccggaa gaagctctgt
     5641 agatgcagaa gcgccaataa atgaaagccg atcttggact tgattggcta aagccgattg
     5701 caaatggtaa agagtcaggc atgctatcaa taaaaaagca gaaaagcttg aaaataaaca
     5761 atagagtatt gtctgctttt tgtaataaat taatcgagca gtagcatagc ggattatttt
     5821 catacttttc acctctataa aaagtataga aaatgaatat gaataaagta tgaaattcct
     5881 ttagtgaaat cacgaattaa aaccagaaaa tattttgtaa aaagcaagag gttgtgaaga
     5941 agctgcccta ttctattacc gtagatgcaa gcttttgaac actgtttatt cgaaaagagg
     6001 agcagcgaca ataattttgt cgctgctcct tttaaattta ttttatcatt cggaagaact
     6061 tgtggtcgtt ccgtgaagtt cttggtacgt ttgcgtttta taaagatcaa cagttgattg
     6121 tccgttgtgt tcactaaaga cactagttga cttgtcacca agagattttt cagcttcttt
     6181 taatttctct aagccattct tgtagtcata atcactggta tcaattgggt ctaatccact
     6241 attcgtatag aaccgaagaa gatcaccatt attgatttgg tcagaaacag ctaactgttt
     6301 atcaactttt tctttcagtg aatcaatttc attctgtact tcctcagtag gttcagtaat
     6361 agcttcacca gtttcgttac tatagatcgt accaccgtag taagtatatt ttggtgtaac
     6421 aaaatcacca tcacggaaag aaacaagctg ttcatgatct ttcgagaata gatcttgtcc
     6481 taactgaata taattttttg tatctacacc tagtaagtga agtaatgtag gcaaggcatc
     6541 aacttgtccg ccgtaagtgt gattgattcc gccctgtgtt tgtccaggga tatggatcat
     6601 ataagggaca cgctgcattt gtgcattgtc gtagtttgtc caagtagaac ttgtttttcc
     6661 aactaattca gctagatttt tatttcttga attcgagaca ccgtaatggt caccgtaaag
     6721 gacgatgact gaattatcat acaagccgct ttctttcaaa taattgaaga attcttgtac
     6781 ggcggtatct aaataattgg ctgtcgcaaa gtaaccgttg atcgtttcat catttgtatt
     6841 ggctaacggg aatcctgctt cgtcattggt gaattttgaa taaggataat ggttagatac
     6901 agcaatgaat tttgaataaa atggttgttg cagacgttcc aagtattgta cggattgttg
     6961 gaaaaatggt ttatcatgca acccgtattg gaacgaattg tcatcattaa catcataata
     7021 agaagaatca aagaaataat catatcctaa attcttgtat gtctcgtttc gattccagaa
     7081 tgtaccagcg ttcccatgga ataccgcact aatgtaacct tgtgtttgac ttaaaatatc
     7141 aggtgcagct tggaaagtat tttttccgcc taattgtgtg aacaaggagc cttgattcaa
     7201 gccaaacaat gaattttcca tcagcgtttc cgcatcgctt gtttttcctg cttttacttg
     7261 atggaaaaag ttgtcaaaac tgaacgtgct gttgctgtgg taaagactat tgatgaaagg
     7321 tgtaacttca tgctggagac cattctcatc tgtcaattta taatcgatca ggaattgctg
     7381 gaagctttct aaatggatat aaatcacatt ttttcctcta gcgagcccga acaaatcgtc
     7441 atttggtgct gcataatgtt cttttacata attttctact tctttcatat cattgggact
     7501 tgcttctgcc cgcacttggt ttgtctggta agtttgaaca ccgtcataaa ccatgaaagc
     7561 attcaagcca agatatttga ccaaataatc acgagagaat gttcgagtca gcaattctgg
     7621 acgatcagct tccgccataa aaagatttcc tgagaaaatc ataactgcta atgtagagat
     7681 cgcaaaagcc attcttgctc ggatcgggcg agggtccaac ttgatttttt ttgcaatcag
     7741 cagaacagtc aagataataa aatcgatcca atagatcaca tcatatgggc gaaacagtcg
     7801 aatggcactt tctcccagac cactcgctac tttcccagca cctaaaatcg tgttgatcgt
     7861 aatgaagtcc gtgaattctc gataataagt gacattcgaa aaaacaagca atgtcaaaag
     7921 aaaataaatg accatcatcg tgatataagc aactctaggg cgtctgatat aaagagcaat
     7981 tccaagtaat aacaatgtag ttgcaatagg gttgataaac aagataaagt attgaatgcc
     8041 actttcgatt ccgagatgga aatcaactaa ataggcaaaa atcgttttca accaaagtaa
     8101 aacgactagt aaagaaaaaa atccgagcct cttattcaaa aaggcaggtg tttttataca
     8161 tttcaagata atcttccttt ctgcacttct taatcacaga agtgtcctga aaccatgtta
     8221 cacagctcac ttattctcat ttattttact agtatgacaa tatagtgtca attgaaacca
     8281 gaagtttatg ctgtttcatt aaggttttct ttaagcttca tgaaaaaatg agcagtaaga
     8341 caagtcctat gttttttgtt atacttggga agagaaagct aaagcaggtg aaggaatgaa
     8401 agtaaaagta aaaaaaagag caagcaatcg attgaaaaaa ggatatccac tgatccaaaa
     8461 agaagacctt gctcatcccc aaaaagaaac cacgcaatgg gtggaattag ttgattcgca
     8521 aaaccaattt atcgcaaaag gatatctagg aaagcagaat aagggaatcg gttgggtatt
     8581 gagccagcag aacatcccat ttgatcaatc tttttttgaa gaattttttg tgaagtcaaa
     8641 agaaaaaaga actttctttt tttcagatga ttctactacc gcttttcgta tattcaatgg
     8701 agaaggagac gggatcggcg gtctgattgt tgattattat gcaggttatg ctgttttttc
     8761 ttggtataac gaaacgttat atgcacatcg tgaattattg attcaagctt ttcgtgaaat
     8821 ctacccggaa atcataggag gctatgagaa aattcgattt gaatcagtaa atctgcctga
     8881 gagccagttc atttacggaa aagaggcacc ggaacccttg atcgtaaaag aaaacggagt
     8941 aaaatttgcc gtttatttaa atgaaggatt aatgactggg atctttttag accaaaaaga
     9001 agtacgggga tcattagcag aaggtttcgc ttctgggaaa acagttttga acatgtttag
     9061 ttataccggc gccttttctg ttgctgccgc aatgggaggg gcaatcgaaa caacaagtgt
     9121 cgatctagca aaacgcagtt taaaaaaaac acaggaacag tttgaagtga atggtttgga
     9181 tgtcgatcag caaaaaatca ttgttatgga cgtattcgag tatttcaaat acgccaaaag
     9241 aaaacaattt ttatatgaca tgattgtatt ggatccacca agttttgcca gaaataagaa
     9301 aaaagtcttt cgcgtagcaa aagattatgg ggaactggtc aaggatagtc tggatatttt
     9361 agcagaagac gggatactta ttgcgtctac aaatgctgcg aatgtctcga tagaacaatt
     9421 ccaaacaatg attgaagagg aattccatgc caaaaatgtt tcttttgaac aacttgcttt
     9481 atatcgtctg ccaaaagatt tccagacgac ggataccttt ttagaaggca attatctgaa
     9541 agtgtttgtt tatcaaatta aaaataacca ttagaaggag cgatgcagaa tggttcgggt
     9601 aaagggattg aatataggaa gcggtagacc aaagatttgt gcgcctctta tcggggccgt
     9661 ccgagaggaa gtattacgtg aagcagttct tgcacgtgaa gcaggggccg atttagtcga
     9721 atggcgagtc gatcattatc gtgaagtttt ttcttttgat gaagtagcag aaacattggt
     9781 tcttatccat gagatgcttg atggactgcc gttattattt acgtttcgta ccttagctga
     9841 aggcggagaa caagacatat cgatccgtca gtatcgcgac ctttatgaac atgtgataca
     9901 cacaggatta gtagatatgg tcgatatcga gttgttcaaa gtagaaagtc taggaaaaag
     9961 actgatagag gaaatcaagt ctttaaacat tcctttaatt atcagtagtc atgattttaa
    10021 agaaacacca gcagaccctg tcttgcttta ccgattgaat gttatggaac attttggagc
    10081 cgatatcgga aaactggcag taactcccaa taatgaacga gatgttttgc ggttgatgga
    10141 gttgactaga cgggcaaatg cttttgtctc catgcctatt attacgatgt ccatgggaaa
    10201 tcttggtaaa atttctcgtc tggttggcgg aacgacgggt tccgttatga catttggttc
    10261 cttaagcagc gattctactt ctgctccagg acaattaccg gtcaaagcat tgaagcaaat
    10321 tattcaatta ttaaatgaac cgttgtagaa caagtttgag gtgatatttt ttggcaaaga
    10381 aaaaaatagt aaaaacgaat gcaatccgta tggtcgaaca aaaaaagatt ccctatacag
    10441 agcacgaata tgagtgggac gaaagccacc taagtgcgtc atcggtcgct gaacagcttc
    10501 cagaaagcca atctcgtata tttaaaacac tggttgctgt gggcaatgtg acggggcctc
    10561 ttgttgctgt gataccggga gaagccgaat tgaatctaaa aaaattagca aaagtttcag
    10621 ggaataaaaa agtagaaatg ctccatttaa aagatttgga agcaacgacc ggatacatac
    10681 gtggtggctg ctcgcctata ggtatgaaaa aactatttcc tacttattta gatcaaattg
    10741 cagaaagcta tgaacaaatc attgtctcgg ctggcagaag agggctacag atggagttag
    10801 cacctcaaga tatctgtgca ttaacgagcg gacaatttgc agatataaaa caataaatca
    10861 tacgtataaa agaaagaagg aggccgggat gttgtttgtc cagtctcctt ctttcttttt
    10921 tatacattat gctctttgcc cggcattcat tcctgctaca tgccctgtag cgaaagccgc
    10981 tgtgatatta aagccgccag tataaccatt gacatccagt aattctcccg caaaatacag
    11041 accatcaatg attttactct ccaatgtttt aggataaatt tcctttagag aaacccctcc
    11101 gccagttaca aatgatttct caataggaaa agttttgttg attgatagct taaactcttt
    11161 gcatagttga acaaaatctt gaatttgttt ttgagaggct tgttgtcctg tcagatgatc
    11221 catttcaagc cgctcaagaa agaaaactaa caatcgctca ggcaatatac catgccaagc
    11281 attgaccaag ttctttttgg ttgctttact tttttctgtc aatatatgaa taagctcttg
    11341 tttggtttgt gtagggaaac aatccagaga aagtgtcact ggttctcccg ttttctctaa
    11401 ttctttattg acaaaacttg aacaacgcag tgcagccgga ccagaaatac cgaaatgggt
    11461 gaaaagtaaa tccatcgtat gttcagtcaa tacacgacct tttttattta aaacgcgtaa
    11521 agtgatgtct tgtaaagaaa gcccttgaag tgttttctcc tgaatatacg gttcatccga
    11581 tatcaagggg gactctgtcg cataaagagg cgtcaccgtg tgacccactc gcttcacgat
    11641 cttgtaccca tctccagtag aaccagtaga tggataagtc cgtccaccag ttgttaagat
    11701 aatggcagga gcatggaact cttcgaattc cgtgcgtata ccgtaaattt tatgttcttt
    11761 gtggatcaat ttctctactc gagtagagaa tagaagcgtc acatctagtt ctttcagacg
    11821 attgagcaat gcttcgataa tcgtttttga tttatttgtt acaggaaaca tccgtccatg
    11881 atcctcttct tttaaatgta cgccttgtga ttcaaagaag ttcatgatat cgaaattatt
    11941 ccattgcgca aacgtactat ataaaaactt tccatttcca ggaatatgtg caatcaaatc
    12001 atcaacagga cgattgttcg taacattgca ccgaccacca cctgtcatca gtagtttttt
    12061 tcctgctttt ttgttttttt cgatcaagag tacgcgtgcg ccttgttcag ctgcactgat
    12121 cgctgccatc atcccgcttg ttccagcgcc gactacgatc acatcataca gtttttccat
    12181 tccctcacct caaggaaagt gtagcatatc tgaagaaaga aaacagaaaa aaatgttttg
    12241 agccaaaaga gaaaatgagc taaaatgagg gaagataagc aaaggagcgt ggaacgatga
    12301 caaacgcaac agaatatgtt caagcaattc aagaaaagct acatcaaaag gatcaagggc
    12361 aagaagaatt tttacaagca atcgatgaat tcatgcccac ggttatgtct tttttggaca
    12421 ctcatcccga atatatcgaa aagaatatct tagaactttt aacggaacca gaaagagtta
    12481 ttcaatttcg agtgccttgg caagatgatg aaggaaactg gcgagtaaat agagggtacc
    12541 gtatccaata caactcagct atcggccctt ataaaggtgg attgcgcttc catcctagtg
    12601 tcaatctaag tatcttgaaa tttttggctt ttgaacagat tttcaagaat agtttaaccg
    12661 gtctgccaat cggtggagga aaaggcggaa gtgacttcga tcctaaagga aaatctgata
    12721 atgagatcat gcgtttttgt caaagtttca tgctggaatt gtcaaaacat atcggcccat
    12781 cgattgatgt tcctgcggga gatatcggtg taggagctag agaaatcggc tatttgttcg
    12841 gcgaatataa acgattgaaa aaatatgata ctggtgtgtt gacaggtaaa cctttggatt
    12901 tttggggaag taagatacgt acggaagcta ctggttatgg attagtgtat tatgtcaaac
    12961 atctattgaa cgaagaaaaa gattcattta atcaaaaaac agtttttgtt tccggaagcg
    13021 ggaatgtagc catttatgca atcgaaaaag tgcaagagct aggcgggaaa gttgttacat
    13081 gttcagattc tagcgggtac atctatgatc cagaaggaat agatgtgaaa ctattgaaag
    13141 aagtcaaaga aatcaataga caacgtttga cggaatacgc aaaccaacgt ccttctgcta
    13201 tttatcatgc tggagaatct gtttggacat tgaaagaaaa agcggatatc gctttgccat
    13261 gtgctactca aaatgaaata gacgaaagct tggccgaaat cttagtagaa aatgggatca
    13321 aaatcgtagc agaaggcgct aatatgccat gtacagtaaa agctgtttct gttcttcatg
    13381 aagcaggcgt gtggtattgt ccaggaaaag ctgctaatgc tggaggagta gcagtatctg
    13441 ctttagaaat gagtcagaac gtccagcgat tgacttggga aagagagcaa gtggatgagc
    13501 agctggatca aatcatggaa acgatttacc atacgtgtcg ccaaacagca gccgagtacg
    13561 gcgacgaaaa aaatctgctt cttggagcaa atgttgctgg atttgaaaaa gttgccaaag
    13621 caatggcagc agaaggttta gtttgaaaaa tgaaactttc ttggaaatat tgtcagcatt
    13681 ttgtttattt gcttgaaaaa taataaaaaa aaacgtaaag tattacctgt agagtaaacc
    13741 tataaggagg agaacaaatg gcacacattc attttgacta ttcaaaagta gcaccgtttg
    13801 tcaacgaaca agagttaggg tacatgcaaa gccaagtaac agcagcccac aacgaattac
    13861 gggaaggtac tggcgcagga agcgacttcc gcggctggat cgatttacca acgaactatg
    13921 acaaagaaga atttgcacgt atcaaagaag cagctaaaaa aatccaatct gattctgaag
    13981 tattagttgt tatcggtatc ggtggttctt atttaggcgc tcgtgcagcg atcgacttct
    14041 tgaatcacac attcttcaat ttattagata gcaacacaag aaaagcacca caaatcttct
    14101 ttgccggaaa ttcaatcagc tcaacatata ttgctgactt gatcgaagta atcggtgatc
    14161 gcgacttttc agtaaacgtt atttcaaaat ctggaacaac tactgaacca gcgattgcgt
    14221 tccgtgtatt caaagagctt cttgttaaga aatatggaca agaagaagca aacaaacgta
    14281 tctacgctac aacagataaa gcaaaaggtg cagttaaagt agaagcagat gcagaaggtt
    14341 gggaaacttt cgtgattcct gatgacatcg gcggacgttt ctctgtatta actgctgttg
    14401 gtttgttacc aatcgctgtt agtggtgcag atatcgatgc attgatgcaa ggtgccgctg
    14461 atgcaagcaa agcatactca agcgacaaac tagaagaaaa cgaagcttac caatacgcag
    14521 caatgcgcaa cattctttac cgtaaaggca aagtaactga gttgttgatc aactatgaac
    14581 caggaatgca atacttctca gaatggtgga aacaattgta tggtgaatct gaaggaaaag
    14641 accaaaaagg catctatcct tcaagcgcaa acttctcaac tgatttgcac tctttaggac
    14701 aattcatcca agaaggacgt cgcaatattt ttgaaacagt tgtgaaggtt gaaaaacctc
    14761 gtaaatcaat cacaattcct gaacaagcag aagatttaga cggtctaggc tacttgcaag
    14821 gaaaagaagt ggacttcgta aatacaaaag catttgaagg aacattgctt gctcatacag
    14881 acggtgacgt gcctaacttg ctagtcaaaa ttccagaaat ggatgcttat acattgggtt
    14941 atacaatgta cttctttgaa attgctgtag gtatttcagg ttacttgaat ggaatcaacc
    15001 cattcgacca accaggtgta gaagcttaca agaaaaacat gttcgcactt ctaggcaaac
    15061 caggctttga agatcttgct aaagaattga acgaacgcct gtaagataag ggtttttcgc
    15121 tagatttaaa taatcagtga tagaaacatg ttcgctttaa aatgaaaact ggtacccatt
    15181 taggtactca aacatactgc atcgtttatt cagaataagg ggctgtgaca agacgtttgt
    15241 cacagtccct tgtttgtata gaaatcgatt gattgccttg ctctttagtt tgttttagga
    15301 ctttaagaac agtgaggctg acttcgaagg ttaattctgt ttttttgttt tctagaatca
    15361 gtaagcaaaa aagcactcac ttcgagtgct tttttgcgat atattcttag cttaagatag
    15421 gcctaatttt tcttttacag atgagggtac cttatcgctg gtaattactt ttttctctat
    15481 gatccgcttt tcgcttgctt tcaactgcaa gatcgttcct tcggggtaag cagaaccatt
    15541 ggcagagtca ccagaagtaa tgacatcgaa atctaactgt cttttttcac cgttttcagc
    15601 ataagcatct acgttgtatt taaaacctct tccaattact tccccattat ctgctttgat
    15661 atcagtttct gcaggcattg gagctttgac taccgcataa taatctttac ctacataagt
    15721 agactgatag tagtcccaac ctttccatcc tataaaagca atcaatatga taacgatcaa
    15781 ccctaaaatt tttttcatga aagatctcct tttttatttt t
//
LOCUS       DACDYI010000073        15071 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.073, whole genome shotgun sequence.
ACCESSION   DACDYI010000073 DACDYI010000000
VERSION     DACDYI010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.073"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(118..432)
                     /locus_tag="IWM52_002291"
     CDS             complement(118..432)
                     /locus_tag="IWM52_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAP9974519.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(446..706)
                     /locus_tag="IWM52_002292"
     CDS             complement(446..706)
                     /locus_tag="IWM52_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAP9974520.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            876..1190
                     /locus_tag="IWM52_002293"
     CDS             876..1190
                     /locus_tag="IWM52_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP9974521.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            1192..2847
                     /locus_tag="IWM52_002294"
     CDS             1192..2847
                     /locus_tag="IWM52_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9974522.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            2930..5065
                     /locus_tag="IWM52_002295"
     CDS             2930..5065
                     /locus_tag="IWM52_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9974523.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            5240..5749
                     /locus_tag="IWM52_002296"
     CDS             5240..5749
                     /locus_tag="IWM52_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974524.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            5746..7050
                     /locus_tag="IWM52_002297"
     CDS             5746..7050
                     /locus_tag="IWM52_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9974525.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            7146..8015
                     /locus_tag="IWM52_002298"
     CDS             7146..8015
                     /locus_tag="IWM52_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9974526.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(8127..8447)
                     /locus_tag="IWM52_002299"
     CDS             complement(8127..8447)
                     /locus_tag="IWM52_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAP9974527.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            8601..8996
                     /gene="dhaM"
                     /locus_tag="IWM52_002300"
     CDS             8601..8996
                     /gene="dhaM"
                     /locus_tag="IWM52_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAP9974528.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            8998..9981
                     /gene="dhaK"
                     /locus_tag="IWM52_002301"
     CDS             8998..9981
                     /gene="dhaK"
                     /locus_tag="IWM52_002301"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAP9974529.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            10009..10614
                     /gene="dhaL"
                     /locus_tag="IWM52_002302"
     CDS             10009..10614
                     /gene="dhaL"
                     /locus_tag="IWM52_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAP9974530.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            10761..11426
                     /locus_tag="IWM52_002303"
     CDS             10761..11426
                     /locus_tag="IWM52_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974531.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            11709..12368
                     /locus_tag="IWM52_002304"
     CDS             11709..12368
                     /locus_tag="IWM52_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP9974532.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(12445..14055)
                     /locus_tag="IWM52_002305"
     CDS             complement(12445..14055)
                     /locus_tag="IWM52_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAP9974533.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(14068..14949)
                     /locus_tag="IWM52_002306"
     CDS             complement(14068..14949)
                     /locus_tag="IWM52_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9974534.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
BASE COUNT         5072 a         2747 c         3116 g         4136 t
ORIGIN      
        1 acttgatgca ggacagcgtt ttcacaacct ttgattcacg gttttccaga agtagcttct
       61 tcgaaaaata agccgaaatt tcttgaagct gatcacttct gtcgcaacct ctactgacta
      121 aggcttgata taagcataac cttgggattg cagttcaact aaatcaagga cacctgctgg
      181 tacaacgatt acttgttcag gtaaagaact ttctgaaata tgattagcac gcatagcatt
      241 ggcgcaagcg tgaaaaacga ctccttgcaa atctaagaat tctgcattgg cagagtctag
      301 ataaccggta atcgcgatgc catttacaga tacgacaatc gtcgtttctg gagaagcttt
      361 tatcaggttt tttacgtttt tccctgtttc tgaccatttt tctaattcat caatatggaa
      421 aacgactttc attgaaatca ttcctttact ttttaataat catcttgatc gcttgttcta
      481 gtttagccgc ttgttcttct ggattactat caggtttctc aaagcaattt ttcagattat
      541 ctgcaacgat catgcccatt acgcgatcaa tactagaacg aacagcgctt aattgagtga
      601 tgacatccat acattctttt tcgtcttcga tcattttttg gataccacga atttgtccct
      661 ctgtacgttt caaacggttc aggatatttt tattatcaat ctccataaca cattctcctc
      721 tatttttata attagataat accccttatc ggtattttat gcaaatataa aaagtagtga
      781 aaaaaatgaa gagtcattct ggttgacata ttaataaagc tcctgtttaa tataccccat
      841 gaggtattta taaaaatgta aatgaggtgc agaaaatgaa tttgctattt caatcaataa
      901 aatcgataac agtcccagaa ttaaaggaaa agcttttgga aaatccagcg ttacttgatg
      961 ttagaacacc tgcagaatac cggggcgggc atataaaagg agctaaaaat gttccattac
     1021 aaagtataaa cagatatgat ggagacaaag aaaagaccgt ctatgtgatt tgtcaatcag
     1081 ggatgaggag taaacaagca gctaaagaat taaagaaatc tggttatgat gtagtgaatg
     1141 tacgtggtgg catgaatcaa tggtttgatc gaacagtagg agggaaataa gatgaaaatc
     1201 gttattgtcg gaggtgtagc aggtggaatg tctgctgcta cacggcttcg ccgattaatg
     1261 gaagatgcag aaatcgttgt ttttgaaaaa ggaccgtatg tttcttttgc aaattgcggc
     1321 ttgccttatt atctttcggg agaaatcagt gaacgggaaa atcttcttgt ccaaacgcca
     1381 gaatcattat ccgctcgttt ttgtttagat gtgcgtccaa atcatgaagt gacagccatc
     1441 tttcccgaaa acaaaacggt agaagtcgta catgagggtc aaaaacacat tgaacagtac
     1501 gatgcattgg ttttatctcc tggtgcaaaa ccagttgttc catcgattcc agggataaca
     1561 gaagccgaca atgttttttc tattagaaat gtaccagata tcgataaagt gatgcatgca
     1621 ttagaaaaac agccaaagcg tgccgtgatc gttggtgcag gattcatcgg attggaaatg
     1681 gcagaaaacc taaaaagaag aggtttagaa gtcatggtga tcgaacaagc accacatatt
     1741 cttccaacac tggatgaaga aatggcagct tttatagaaa aagaattgtc tcatcaagga
     1801 gtagaagtga ttacttctca tgctgtcgct ggatttgaag accacgggaa acgattgcga
     1861 ctggatgatg ggcgtaccat ccctgctgat ttagttattt tatccattgg tgttcgtcct
     1921 gataaccagc tagcagtgac cgctggaatc gaattaggca tacgcggggg tatcctagta
     1981 gacgaacgtt atcaaacgaa tattcctgat atttatgcgg tgggggatgc tatcgttgta
     2041 aaacagcaaa tcactggaaa agatgcactt atttctcttg cttcaccagc caatcgtcaa
     2101 ggtagacaag ttgcggacac gatttccgga atttctcgaa gaaatcaagg cggtattgga
     2161 acagcaatta tacgaacgtt tggaatgact gccgcatcca ccggtttaag tgaaagaaca
     2221 gccaaagaaa acgaactgtc ttttgaagtc attcatgtat caggaaaaga tcatgcaagc
     2281 tattatccag aagcaacaga tattttactg aagttgatct tccatccaga gactggcgag
     2341 atttatggtg cacaaggtgt tggggcaaaa ggtgtggata aacggatcga tattttagca
     2401 acagcaatca aagggcattt gacgatcttc gatttaccgg aattagaatt gacgtatgca
     2461 ccgccatttg gctcagccaa agatccagta aacatgctag gatatgcagc aatgaacatt
     2521 gcagaagggc ttagtgaaac cgtacaatgg catgaattgc cgacagaact agcaaaagga
     2581 aaaattttat tagatgtgcg aacagcagaa gaattggaaa agggcaaatt caaggaagcc
     2641 aaacatatcc ctttgaatga acttcgggac cgattaaatg aattagacag ccaacaagaa
     2701 tatatcgtca gctgtcatag cggattacgt agctatctag cggaaagaat cttgaagcag
     2761 tctggctacc acgtaaaaaa ccttgatggt gcattttctt tatatcaaac tgtccgacct
     2821 gaagaactga tatatcctaa caaatgatac tcgttaaaaa gcttgcattc gactcttaaa
     2881 agaggtaaac taatcggttc caccacttta aaaaaagagg agaatgtgta tggtcaacac
     2941 acaaaaacag ttagaagaag catatctgaa gaacgtgtat caaaagctaa ttgataaaaa
     3001 aaacgaactg cgggaatcga tggatacggc aaaaatagaa ggaaaccagt cgttgaaaga
     3061 aatggctggc gagcttcgat tgaattttga cagttactta gataacctgg atactttttc
     3121 gatgattgaa atgaaaaata gagaaatcga tcagatgaat atcaaacaga aaaacgcaga
     3181 aaatcagcta gcaaagatcg aacgtttatt gaaaagtccc tactttggaa aagtcgttgt
     3241 agactttctg gataatgagc caaaagaatc cttttatatc ggtacagcaa atttcacaga
     3301 tgatgcagat gaaaatcttg tttatgattg gcgttcacca attgccgaac ttttttacaa
     3361 taacatgatt ggtccatctt cttatacggt tcgtcaaaat aaaatcgata cggttattaa
     3421 agaaagacga caatttcttt tggaaagaga tcgtttggtc aagttttttg atacgtctgt
     3481 agctattcaa gatgacgtat tattagaagt cttaggccaa gatgagacca atgaaatgaa
     3541 ggctatcact gctacgatcc aatcggaaca aaacacaatc attcgggatg tgaaaagtca
     3601 aaatatcttg gtaaatggcg tagcaggaag tggtaaaacc tctgctatca tgcagcgtat
     3661 tgcgtatctg ctctatttat atcgagatac gatgacatcc gatgacttgc tgattctatc
     3721 acctaatcat cggtttatcg actatatttc gaatgtccta ccatcattag gtgaaagaaa
     3781 tccactgaat ctaaccatca tacagctggt ttcacagctt tcagcagagg aaatagaagg
     3841 tgaagaggct tatttcaaac gtatcacggg agaaaatgta tcggagcaga cagaacgttt
     3901 gcgtagcaaa aagtttatcg ataatctaaa acaatcagat cctttgtttt tagatcatcc
     3961 gaattttatc cgtggactta ctaaaaatgg aaaaacagtc ctttctaaaa aaacgatcga
     4021 aaaaatatat gagaaagttc ctgctcatcc taaattgatc gataggcttc aagcaacgaa
     4081 aaaggcactg atgagcgaat ggaagaatca tcttttgaaa caagcaaaaa gtcctgctgt
     4141 gcagaatcag gtactctcgt tgacagaaga taggcagttg gaattattcg gtaaattgat
     4201 ttctgatgat tcggaacaaa gtattgtagc ttatgcaagg aaacttttac aaaaaaaata
     4261 tcgaaaaata accagacaaa tcgaagaaat ggcatgggtg gatgagcatc agttgtttga
     4321 aagaatttat gaaaaacgat atggctcagc ttatgcttgg caacccacac gtactgttga
     4381 tgaagcagtc attatattag ctattcgtca tttacttgtt gaaaaggtaa atgtaccagc
     4441 atttcgctat ctattgattg atgaagtaca agattatacc ctggcacaat taggtctatt
     4501 gatcgaatta tttccaaaaa ctcactttac tttagtaggt gacgagaatc aggcaatctt
     4561 taattcttcc actacttttg ctgatattat gcgttgtttc gacgactacc atctgccgat
     4621 ccatcggtat gatcttagaa atagttaccg ttctagcgga gcaatcacag agcttttcaa
     4681 aacctatgct gtggatcaag agaagatcga catcgtatcg atccgaccgc aaggaaaaga
     4741 acccgaatac cgctcttttc gatcctctga agaattgttg aaaatactag ctgagatttt
     4801 ggtttctcta aaaggagaaa aagcggcagt tatcacccaa acagaagagg aaacccaaac
     4861 gctacaagaa tcgattaaaa aaaattcata tggattagaa aactgccaaa tcattccttt
     4921 gagtctagca aaaggactgg aatttgatca tgtaatctta tatccatttg aaaacgatgg
     4981 agatgagcaa agaagacgca gacaaatgta cacggcgata tctagaggaa tgaaaagcat
     5041 tgtcgtattg gaaagagcaa cataattaag agataaaaaa taaaaagaac aactgataaa
     5101 aagaacaaaa tatttttcat ttgctgatga gaaatgtttt gttctttttc ttaggaaaaa
     5161 tttaggaaca ctttatgtga aaagaaatga aatgtatggt aaactcctac gtcgtaacaa
     5221 aaaaacgtga ggtgaggata tgaaaaaagt aacaagagaa gaagttgcat caattttggt
     5281 gaaagataag tatttttcaa aaggtaatta ctggttaaag atttggcaaa cgttagtggc
     5341 tcttttagga tggatgatcg ttgttcttcc ttttatatgg gtctttttcc cattaacgag
     5401 accagaaaga gccaaggatt tttccttgta cgctttttta tcagaaaaga aaatgctgta
     5461 tttcttaatc ggcttttttg tattgatttt tttcagtttc ctgattacct catttgtatt
     5521 gactaggtgg aacaatcgaa atctatacaa aaaagtaaat aaatcttttg aatatgaaga
     5581 tgaaaaattg aaaaaaagac aagaattact tgaagaagtg tatacggaac gttttggaac
     5641 aaaagaagag agagaaacgg tccgttttta ttctgtctcc gaagagaaaa atctagatac
     5701 gacattcatt gcagatttgt ataaagaaaa tggggtggaa ctgaaatgat ctcaatcacc
     5761 catacaggta atttattctt agatacttgt ttgtctatta tgtatttttt tctcgtttct
     5821 tatccgattt taggcggatt tgtatggttt attggtgtct ggtgttatgt gtttttatac
     5881 aaacataagc aaaaagaatg ggtagatgtt cccttgagtg tcgaaccgtt catcacgatc
     5941 atggtgccag cccataatga agaaattgtg atcgaatata caatcgagta tttgatgacg
     6001 aaattgaatt atcacaacta tgaggtatta gtgacagacg atggttcaac ggaccaaaca
     6061 ccagaaatat tagctagact gatgaaaaaa tacgctaatc ttcgtgtggt tcggatcgaa
     6121 aaaaataaag gaaaagccca cgcgtttaat atcggacttg cctttgcaaa agggaaattg
     6181 attttaagca atgatgcaga cacggtaccg gagccagatg cactgattag gtatgtgaat
     6241 tatttcattc gccctggtgc aagacatatt gctgcggtca ctgcaaatat ggatgtccaa
     6301 aatcgtacca aattgattgc aaaatctcaa acggttgaat tctcaagtat cgtgggcatc
     6361 attaaacgaa cacagtcagc tgttttcggt ggtctttatg cgtacagtgg agcaaatacg
     6421 atgtatcgaa aagaagcctt gatcgatgtg ggcggattca gacaagatcg agcgacagaa
     6481 gacatcagta ttgcttggga tcatcagtta aatgactggt tatctgtttt tgctcctgag
     6541 attatctttt tcatggaagt acctgtgacg ttgaagatgc tatatcgaca cagaaaacgt
     6601 tgggcaaaag gtggaacaga agtctggctg acgaatttca aaaaagtgat gctccatcct
     6661 tttaaacata ttggaagaac gatcatcttc attgatcaaa cattgagtat tgtctggtca
     6721 atattctttt gtatttccgt agttcttttt gctggattaa tcgggcatta tgtatatcaa
     6781 ggaaactacg aacagattta tattactttt acattcagtt ttgtgttcat ctgctttgaa
     6841 atggtcgcag gatttttcca gttgctagct tcgctgattg tagatgatcg acgaagaaaa
     6901 ttgaaatacc ttcttttcgc accactatat atgcttttgt tctggatagt gaacgcgata
     6961 acgatcgtga cgactttcat tccagcagtc aaaacgattt taggttatgg aagcgggaca
     7021 tggaaaagcc cggaacgtac aaaaaaataa aagatgcacc gttttttaga caaacagcct
     7081 gttgtgttta tggttcttcc aaacattttt gcttataata agaaattagt taaaaagtag
     7141 taaggatggg aaaagtgatg aaaaaaagaa tgaagccgtt aatctggcca attgctgttt
     7201 ccagtatttt cttattggga cagacagctt ttgcggaaac ttctgaacct gctcaaacag
     7261 aaccttcaac aaccataagc tcttctggtg ctgaaaacgg tgcctcggaa acagaaatga
     7321 cttccaatgt accgacagat aattcaggag aaacgatttc atcttcagaa gatacatcaa
     7381 taaatagttc tacctcttct gaccaaatcg atcatttgca agacttggaa aataaaagaa
     7441 aagaactcga agaagcatta gaagctgcaa aaaacaaatt gaaagaagac caagcagcaa
     7501 tcgatcaaga tttagctgat cttaaaaata aagtgggaaa tgaccgtgca caacttgaga
     7561 aaaagcttgc cgaagcgaaa gcaaaagcgg aagctgtcca aacagatctt gacgggaaga
     7621 tccaacaagc aaaagaattc aatcagcaat taaatcaggt tgctcaaaca gcttctggaa
     7681 agctaagtca aactcaagcg gatatcacag cggccagtca agcaatccac ggaaagatca
     7741 gcgaagcaca aacagttgct aatgattttg aagcaaaaaa agctgactta gcagctagtt
     7801 tccaacgaat aaaagatatt ttgcattcgg caggagaaaa atcagtgacc tctggaagca
     7861 cgacagaaac agttcagcaa acgacagagc catccaaatc aacaagtcag actttgccaa
     7921 aaacaaatga aaaaacagct tctgtcagtt attccttagc aggggcgggt gtgttagtcg
     7981 cagcagctta cggaatcatc cgcaaaaaga aataaaaaag agaggatggg aaggtagagt
     8041 acctcgaagt tagactgaaa aatctaattc ttgagtgcca ctacatgtcc catctctctt
     8101 tagttattaa aacagatgtc ccctacttat ctcgtggaga cagctgttcc actacatgtg
     8161 accatcagca tgccattgtc gctgcctaat acttcatagt ccatttttac gccaatgatc
     8221 gcatcagctc ctaatgcttt tgcacgttcg gccatttcgg caagtgcttc ttctcttgca
     8281 cccattagtt cgttttcata gctttttgag cggccgccaa agatatttcg caaccccgcc
     8341 tccaagtctt taaatgcatg gatcccagta atgacctcac caaaaacgat ccctttataa
     8401 gctactacgg cttttccctc tacactgttc gttgttgttg taatcattcg atcactcctc
     8461 tcttttaatt aaagtataac aaacttcaag aagcactaca catattttcc caaaattaga
     8521 aatttattat atacttgaat tgttcattaa tgaaagcgaa accaatcgtt agcaactatg
     8581 taaaagaaag gaagattctt gtgaaaaaaa gtattttgct tatttctcat agtcaaaaag
     8641 taacagatgg tattaaagaa atgattgagc agatgcaaaa atccgaagag gtgcaaattt
     8701 tttctctcgg cggaataaat ggcgagatcg gatcagaccc gatgaaaatc gtagaagcag
     8761 tcaatgaatc aagcgatgca gctagttatt ttgtttttgc ggatatcggt agcgcggtat
     8821 tgaactcgga actggcaaaa gacatgttgg aagaagagca gcagcaacgt tatcatctgg
     8881 tggatgcgcc tttagtggaa ggagcttttg cagcagccat cactgccggt ataacagatg
     8941 atactcagca aatcttaaca gaagcacaaa atgccggaaa gaaaggatgg gaataaattg
     9001 aaaaaaatca tgaatgatcc aagcaatatt gtcgaagaaa tgctggaagg attagtaaaa
     9061 agctatccag aacttgttca tcgtgtagaa tcttcccgtg ttgtagccaa gaatcagaaa
     9121 gcggaacaag ttggtttggt ttctggtgga ggaagcggac atgaaccatc ccatgccggg
     9181 tttgtagggg aagggatgtt aagtgcagcg gtgcttggtg atgtgtttac ttctccaact
     9241 ccagatcaga ttcagacggc tattaaggaa gccgatagtg gaaaaggagt tttgctgatc
     9301 gttaagaatt atacagggga tgcattgaac ttcgatatgg caaaggaatt agctgctatg
     9361 gacgatattg aagtagaaag tgtgatcgta gatgatgata tcgccgttga aaatagtacg
     9421 tatactgctg gaaaacgcgg agttgcaggt actgtccttg tacacaaaat cgtgggcgat
     9481 gcagcaagaa acggtgcttc cctagctgag ttgaaagaac tgggagaaaa ggtcgtacag
     9541 gcaactaaga cgatcggtct agcgcttcga gcagcaactg taccagaagt agggaaacca
     9601 ggattcgaac taggagaaga tgagatcgag tatggtgttg gtatccacgg tgagccaggc
     9661 tatcgtcgag aaaagatgca gccttctaaa gtactggcaa aggaacttgt tactaagatt
     9721 ttagatgatt actcgaattt accaaaagaa gcaggtgtac tagtcaacgg aatgggcggg
     9781 acgcctttga tggaacagtt cgtgttcatg aatgatgttt tagctctgtt ggaagaaaga
     9841 ggcgtcaatg tagttttccg aaaagtcggt aattttatga cctcactaga tatgcaaggt
     9901 ctttctctaa cactgatcga cttgacggag acacaatgga aagattcttt agaaagcaat
     9961 gtacagacga tttcatggta aaaaatctca agataaaagg gtgataatat gaaactaacc
    10021 gttcaagaaa tccaagaatg gctcacgcaa tttgcagagg caatcaatca gaacaaacaa
    10081 tatctcagtg atctagatac cccgattggc gatggagacc atggaaataa catgggaaga
    10141 ggggtttcag cttatgaaga agcttttcaa gccgatcatc ctgaaacaat cagcgataca
    10201 ttcaaagtat tctctatggc gatgatatcg aaagtcggtg gggcttctgg tccgttatac
    10261 ggatctgcat tcatgaacat gatgaaagcg acaaaagagg tcgattctat tgattctcaa
    10321 gaaaaattag gcgaagtgat cgagcaggga acagcgggta ttcaaagccg aggaaaggca
    10381 gaagctgacg ataaaacgat gttagatgtt tggctgccag tcacagaggc attgaaggca
    10441 gggaatttaa caaaagaaat cattgaccag gcaaaagaac acaccaaagt cttggtagct
    10501 aaaaaaggac gagcttccta tctaggagag cgtgcaatcg gacatatcga cccaggagct
    10561 gcttctagtg ccattttatt ttcaacgctt ttagatgtga tcaatcaacc ttaagttcaa
    10621 taaaactgcg gtgatgctca tcgcagtttt ttgtttggct gttcttatga cttgatgcag
    10681 aacagctttt tcacaacctc tatttgtcca tatcgcaaaa ttcaggtaga atacaaaaga
    10741 taacggaaag aggcgaaaca atggaacttg gaaaatcaga ttatcagttg tttgatcgac
    10801 caatctacgc attcaaacag ctcaaagaga gccatccaac agataagatt gaacaaatca
    10861 agaaagaata taaagaacac tggcaaaaat ggaaagagat tcagctccag acagctgcac
    10921 ttttgcctga tatgtacggc atgagcaaac ctaaaatcga aagctggacg aatggctgga
    10981 atttacgcag tcatttttgg agcgcgtacc gaagcgaatc tcgtcaagac gagaatgcct
    11041 gtctagcagt tttgttgaat caaaaacaat accaaatcta tttgatgtac caacattata
    11101 aaagcgaaga acgatatggt agcattgcag cgtataacca gttgcttgcg atactgaaag
    11161 agtggagcaa aacagtcgat atcaaggatt attacatatg gccgcaacca gaacatgaac
    11221 ttgacgacca cttagctttg tctgactatt tgtccgatac aaagaaacaa gaagagttga
    11281 aaaaagcgat gagagaccga acctttcaaa tgggtaaact attcttctat ccgcaagaaa
    11341 tcgaacacgt tgagcaaata accgctgaaa ccttacaaga actagcacca ctatatcaaa
    11401 agctaggtgc ggaaaaattt caatagacaa cacgaaataa gcaatgcgaa aaacaaacct
    11461 tactctcggc ttgcgagtga aggtttgttt ttgattattc aaaaagtaaa tatttaaaaa
    11521 attgatagga atcaattact taaggtctca aaagaaggaa aataaagcaa gggacaaaaa
    11581 gaaatccact ctatttgctg gtatctggtt cgcttgacgt ttatgctatg ataaaagaaa
    11641 attatcagtt ggaataaggg aagttatgtt tctttattag aaatagctgt ttagaaggag
    11701 gagagattat gagtcatgat catttatgtg tttcatttgt tcctttgttc aatcacttag
    11761 tacagcaaga tcaagaaaaa gtacacgcac ttgtcaaaca tcactatgtg gaaaaaggag
    11821 aacagattct tgcgccgaat ggagaacctc aattagtgat cgtggcctcc ggcagtatga
    11881 aagtgtatca attatctgct agcggaaagg aacagctttt acgagtaata gagccgggcg
    11941 gatatgaggg agagagccag ttgctaggcg ttccaaatga gtctttattt ggcgaagcac
    12001 tagaaaaaac ggaaatttgc atattgagac aaaaagattt ctcggaactc ttactgcatt
    12061 atcccgccct tagtctgaaa cttttagcaa tcaacgttga gaaattaaca aaagtacagc
    12121 agcagacaac ttttttgatg atggaaaaag tagaagaacg agtcgctgtc tatctgcttg
    12181 acttagctaa agcgacaggg aaggatcact ttttcctgcc aatgaaaatg aaagaacttg
    12241 ctgcatttat cggtacgacc cccgaaactt tatctagaaa atttaaatta ttagaggaga
    12301 atggttatgt aaaaagaaag aatcgagaag tctggatcat tgataaagag cagttagaag
    12361 acttgtaaat aaaaaaatga cctccaaatg ttggattttt gacccagatt tggaggctag
    12421 catgaaattg tacatgcttt tttattatcc ttctagtaag tctcttcgcc tataaccaat
    12481 agaaccaagg acgaacagtg caaggctgat acatgataac gtaaggaaca cggaaagatc
    12541 aaaagaatcg tttggcatcc gtggaaccca gctagggaca gctgttttgg aaaaccattc
    12601 tggtaaatcc aaaattccgc caaagtaatt gagtgcaaag gagtaagcta agtagatata
    12661 agcaagttta ccgattcgag gaagccatcc gaatagaaag ccactaattc cgataaacac
    12721 taaaacggca ggcaggaaat tgaacccggc tttaagaaac atcaatagat ccatgtcttt
    12781 cgcatccttc atcgaagcca aagcagaaag ccctagtgca ccagaactaa tcaggattgc
    12841 caatatagca aatagaatag ctagtaacat attggtccaa taaagttggt tcctagaaat
    12901 ttttgttgcg aacagctgat tcatacgtcc gtgggtttct tctgtaaata aacgattgat
    12961 gctagcaatt ggtaaaatag cagctagaca aatcaagact accatgatcg tgcttgtaaa
    13021 gtttttttca attgagacgc cagattggat aaacatctgt ttcattaagt cgttactttc
    13081 taaaaactgt tgcatattcc cgtagataga gccgtatgcc gcccccatca aaccaaaagt
    13141 gataagccaa ctgattgtca tccctttatt tagatagaat aataaaccac gcaaggataa
    13201 aagagaacgt ttagcatacg gttttccttc acgttcgggg atatagccgg tgttcaaatc
    13261 tcgatgttcc tctaaaacaa atgaactgat tatcaagatt agactgaaca aaaacaaata
    13321 aagcaaagga atccagttgt tagttgtaaa tggataggtc aaataagacc agcctaaagg
    13381 attccacatc gaaagggagg aatcagctac atctgttatt gctcgtagaa ggtaaagtcc
    13441 acctaaaaac gctaaagaca gaccattcga actggctgaa cttggcatca gttgtgcagc
    13501 aaacagtgca acggcactcc ccaaaatccc catactacct acagatagcc caaaaagcca
    13561 agcaccttct gcagtgatcg aatccgcttg gaaaaaaatc atcgtacagc tgatcgatat
    13621 tgctaaaagc aaattgatca gaatactttc agcgatgaca gctaaagagt ttgcttgacg
    13681 tccgactcga aaggaacgaa taagttcaat cagaccaagt tcttcttttt ttctcgtttg
    13741 gctcaccacg tgtaaaacag agatcacagc agcaaacaaa ctgcaaaaaa gcaacatttc
    13801 atttgcatac atcgctccta aagtatactc agcagcattc gaagctggag ttggtccgac
    13861 cattgcaatc attgccgggt tttgcaaggt ttcgtataat ccagctaatc cttgtccgga
    13921 agtcaattct tcaaacgcag ggacaaaacc accagaaaaa gcagtgacac cgactagcca
    13981 tatcaggatc tttttccagt cacggctaag gttttgagaa aaaagggtgg accagcgtga
    14041 aaatttatca gtcatttcgc ttcctcctca tccttcataa tgtctcatga ataaatcctc
    14101 aagagtcggt ggaatagatt caaatttttt cacacctaat ttggacgctt cagctaaaat
    14161 cacatcgaaa gcttcgttat cagcagcaaa ttgagcgagg tgaccttttt gcacaaagtc
    14221 atggacaccg ttcaattgtg ctaagtgact attctctgct tctgtttcta tagtgatggt
    14281 cgaacgagtc aaatgacgca attcatcgag tgttccttct tcatcgagtg ttccttcgcg
    14341 tatgatcacg actttatccg ctagtcgctc gacttcactg agaatatgag aagaaagtag
    14401 gatggatttg ccctgttctt tcaatttttc aacttcttct tggaaaatcg tttccatcaa
    14461 aggatctaaa ccagaagtag gctcatcaaa taaatagaga tccgaatcaa cagccaaagc
    14521 agcaatcaag cccacttttt ggcgatttcc ttttgaatag ttttttgctt ttttcttagg
    14581 atcaagagag aaacgttgaa tcagttcatc ttttttttga gcactccctt ttccatgaag
    14641 cttcatgaag agatcgatga tcgctcctcc agtcaaattt ccccacaaag agatatcgcc
    14701 tggaacataa gaaagacgtt catggataga taaactatct tcccatacat cttgcccaaa
    14761 aatcgttgcc gttccgccgt ctttttttat aatccctaaa agtgtccgaa tcgtcgtgga
    14821 ttttccagaa ccatttggtc cgataaatcc aacgacttca cctgaatgta cggtgaaaga
    14881 gacatctttt aaagcttcga aacgaccaaa gctctttttc aaatgtgtga ctttgacgat
    14941 ttctgccata aaaaccccac tcctttttct aaaaatatat actaaacaag attaaatagt
    15001 ttaataaaaa ggttttatac ctttttataa aacttttttt attattttag tacataatat
    15061 tctttttata a
//
LOCUS       DACDYI010000074        14062 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.074, whole genome shotgun sequence.
ACCESSION   DACDYI010000074 DACDYI010000000
VERSION     DACDYI010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.074"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IWM52_002307"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IWM52_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAP9974535.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IWM52_002308"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IWM52_002308"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAP9974536.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="IWM52_002309"
     CDS             complement(2169..2906)
                     /locus_tag="IWM52_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP9974537.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="IWM52_002310"
     CDS             complement(2997..3524)
                     /locus_tag="IWM52_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAP9974538.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="IWM52_002311"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="IWM52_002311"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAP9974539.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="IWM52_002312"
     CDS             complement(4401..4886)
                     /locus_tag="IWM52_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAP9974540.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="IWM52_002313"
     CDS             complement(5039..5236)
                     /locus_tag="IWM52_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974541.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IWM52_002314"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IWM52_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAP9974542.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IWM52_002315"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IWM52_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAP9974543.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="IWM52_002316"
     CDS             9211..9423
                     /locus_tag="IWM52_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAP9974544.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="IWM52_002317"
     CDS             9425..11104
                     /locus_tag="IWM52_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP9974545.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(11358..11558)
                     /locus_tag="IWM52_002318"
     CDS             complement(11358..11558)
                     /locus_tag="IWM52_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9974546.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(11708..11869)
                     /locus_tag="IWM52_002319"
                     /pseudo
     CDS             complement(11708..11869)
                     /locus_tag="IWM52_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            complement(12249..12322)
                     /locus_tag="IWM52_002320"
     tRNA            complement(12249..12322)
                     /locus_tag="IWM52_002320"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12286..12288),aa:Ile,seq:cat)
     gene            complement(12634..13032)
                     /locus_tag="IWM52_002321"
     CDS             complement(12634..13032)
                     /locus_tag="IWM52_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974547.1"
                     /translation="MDKGNSVTFALENISLNKIHFELTDNFSYGDLEFNIELGYKEDE
                     AEYLEKKLVSLRAHIVGGMEGAPVDILLFSVIRVSTNEQPYKKLVEESMGELAKPILN
                     KASNLISNLYSEVGVMPTVIDLYQLYSQQK"
     gene            complement(13025..13477)
                     /locus_tag="IWM52_002322"
     CDS             complement(13025..13477)
                     /locus_tag="IWM52_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974548.1"
                     /translation="MKSINDLLKKHPDLYKKLNSEQRNLSKQLLKIRLKKQKNVTEFS
                     KMLGISEKKYLEFEFGDMHIPVAEYNKLLKQVENLSYKDSMNFTIKVAGGPIKYDRIE
                     NKPYIRNKSYSVFKSRKRKEVSSNMVYASVNYQDTSRYIVEKEVYVHG"
     gene            complement(13464..13871)
                     /locus_tag="IWM52_002323"
     CDS             complement(13464..13871)
                     /locus_tag="IWM52_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974549.1"
                     /translation="MSFLLLKYLEIYSVKKNKLIGENDSPIKTPIELSLRNRMGILQR
                     SEIVKELFYHKFSYEIRLDDTYEHYRIVFFVYNSIEDATATTALTFGFTKNGTINSDK
                     TRQAATESDGICKKVCNGEENYWIGEEKLNEIY"
BASE COUNT         4151 a         2947 c         2366 g         4598 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaatacctgc cacaagagaa attgtctctt
    11281 gtggcaggta tttttgttac tcgctttgtt ctctcgtaaa aattgatgtc taccatcaat
    11341 ttgatttaca gataaaatta agcttttaca acgtttgcag cttgtgggcc gcggtctgat
    11401 tcttcaacgt cgaaagtcac tgcttgacct tcttctaatg ttttgaatcc gtcaccttgg
    11461 atagctgaga agtgaacgaa tacgtcgctt ccatcttcac gtgagataaa tccaaatcct
    11521 ttttcagcgt tgaaccattt tactgttccg ttttccataa taatatcctc ctcgtgccaa
    11581 aagcactaat ttaaattgta acattgctcg tgtacgatag aggtgcaaca gatgtttcaa
    11641 cttctattcc caaacaaaat tacaatataa ttgtacactt gcttcatttt ttttgcaaga
    11701 aagcaactta cttctcttcc atcattcctt cttctctcaa actaaaatat cgtttgcgtc
    11761 cgatgatcaa gtggtccagt acagtgatcc ccatcatttc tccacacttt tgtatccttt
    11821 tggtaaaatt catatcctgc tgcgatggaa taacattacc agaaggataa tataggttag
    11881 tacacgtata aaaatatctt ttatattgac acaatttatt taacaagtat aataataatt
    11941 gaaagtgaat ttaaacaaat atacaccata aacacaacgt acctgcgaat acgttgtgtt
    12001 tttttctttt tcaaaaaaag acgcttaccg ctgcgaaaca gtaagcgcca ttgtcggctc
    12061 taatgtttat ctagccaatg actaaataat gccaaagtta aaccaattaa caatggcaag
    12121 agcacgtgct ccaaagtgaa ttcaaacaaa tcatacacct cctcccacga aaatctctgt
    12181 ggaagcgacg agccgatatt ttattatacc attattgaat tttatttttt atcaaataga
    12241 ttaaattatg taccctgtag gactcgaacc tacgaccgga cggttatgag ccgtctgctc
    12301 tgaccaacta agctaagggt actggttgtt gccacataaa gccataaaca atcaaccagt
    12361 agaatgtgtg gcaacaaacc tgttatcgca tagcttggag tgtgactatt atgggtgata
    12421 gtgaagatat gcgataacat cactatttta tcgaatgatt tttatagttg tcaatacagt
    12481 catctaaata cttcatatca atcatttatt cagaaacaaa ctatcttgca ggatctatac
    12541 gagttaatta cagagcaaaa aagcctcaac caaggatgac tggttgaggt ctttgtcttg
    12601 caaatttaaa tagataactc tattacttgt attttatttt tgttgactat atagctgata
    12661 taaatcaata actgtaggca taactcctac ttcgctatat aaattagaaa tcaaattaga
    12721 agctttattc aggataggtt tagctagctc tcccatagat tcttctacta attttttata
    12781 tggctgttca tttgtagaaa cacgaataac tgaaaagagt aaaatatcta ccggtgctcc
    12841 ttccatcccc cctacaatgt gagctcgtaa agaaacaagt tttttttcta gatactctgc
    12901 ttcgtcttct ttatatccaa gttcaatatt aaactctaaa tctccataac taaaattatc
    12961 agttaattca aaatgaattt tattcaaact aatattttca agtgcaaaag taacactatt
    13021 tcctttatcc atgtacatat acctcttttt ctacaatata gcggctcgta tcttggtaat
    13081 taacactagc atagaccata tttgacgata cttcttttct ctttctactt ttaaatactg
    13141 agtaactttt attccgaata taaggtttat tttcaatacg gtcatattta atagggccgc
    13201 cagcaacctt aatagtgaaa ttcattgaat ctttatagga caaattttca acttgtttca
    13261 aaagtttatt gtactctgca acaggaatat gcatatctcc gaactcaaat tcaagatatt
    13321 ttttttcact tattcctaac atttttgaga attctgtcac gtttttttgt ttttttaatc
    13381 ttattttaag taattgtttg gacaggtttc tttgttcaga atttaatttt ttatataaat
    13441 caggatgttt ttttaataaa tcattaatag atttcattca acttttcctc ccctatccag
    13501 taattctctt ccccattgca tactttctta catataccat cactttctgt agcagcttga
    13561 cgggttttat ccgaattgat tgttccattt ttcgtaaaac cgaatgttaa tgcagtagtc
    13621 gcagtagcat cttctataga attgtatacg aaaaaaacaa ttctataatg ctcataagta
    13681 tcatctagtc ttatttcata actaaattta tgataaaaaa gttcttttac tatttccgat
    13741 ctttggagta ttcccatccg atttcttaag cttagctcaa taggtgtttt tattggagaa
    13801 tcattttcac caattaattt atttttttta acagagtaaa tttcaagata ttttaataga
    13861 aggaaagaca caattatggt ttaaatttga tccttcgaat agatttatca aagattttta
    13921 taaggtgtgg gattcagaag ttttctttct agcaatcgaa gatagcttat taatcaatct
    13981 ctactattct aataagaact actttaaaat ccctgctgca aaaactagaa tgaagaaaga
    14041 tgtatacttt ttgtttgata tc
//
LOCUS       DACDYI010000075        14000 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.075, whole genome shotgun sequence.
ACCESSION   DACDYI010000075 DACDYI010000000
VERSION     DACDYI010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.075"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..135
                     /locus_tag="IWM52_002324"
     CDS             <1..135
                     /locus_tag="IWM52_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9974550.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
     gene            complement(313..2310)
                     /gene="tkt"
                     /locus_tag="IWM52_002325"
     CDS             complement(313..2310)
                     /gene="tkt"
                     /locus_tag="IWM52_002325"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP9974551.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2411..2662)
                     /locus_tag="IWM52_002326"
     CDS             complement(2411..2662)
                     /locus_tag="IWM52_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAP9974552.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2808..3431
                     /gene="lexA"
                     /locus_tag="IWM52_002327"
     CDS             2808..3431
                     /gene="lexA"
                     /locus_tag="IWM52_002327"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAP9974553.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3611..3829
                     /locus_tag="IWM52_002328"
     CDS             3611..3829
                     /locus_tag="IWM52_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974554.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(4118..4630)
                     /locus_tag="IWM52_002329"
     CDS             complement(4118..4630)
                     /locus_tag="IWM52_002329"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP9974555.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4644..6941)
                     /gene="recJ"
                     /locus_tag="IWM52_002330"
     CDS             complement(4644..6941)
                     /gene="recJ"
                     /locus_tag="IWM52_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAP9974556.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(7083..7517)
                     /locus_tag="IWM52_002331"
     CDS             complement(7083..7517)
                     /locus_tag="IWM52_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAP9974557.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7527..8315)
                     /locus_tag="IWM52_002332"
     CDS             complement(7527..8315)
                     /locus_tag="IWM52_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9974558.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8312..9253)
                     /locus_tag="IWM52_002333"
     CDS             complement(8312..9253)
                     /locus_tag="IWM52_002333"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAP9974559.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9430..10017)
                     /locus_tag="IWM52_002334"
     CDS             complement(9430..10017)
                     /locus_tag="IWM52_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAP9974560.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10238..11551)
                     /gene="obgE"
                     /locus_tag="IWM52_002335"
     CDS             complement(10238..11551)
                     /gene="obgE"
                     /locus_tag="IWM52_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAP9974561.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11839..12849
                     /gene="gap"
                     /locus_tag="IWM52_002336"
     CDS             11839..12849
                     /gene="gap"
                     /locus_tag="IWM52_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP9974562.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12934..13869)
                     /locus_tag="IWM52_002337"
     CDS             complement(12934..13869)
                     /locus_tag="IWM52_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9974563.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3883 a         3130 c         2461 g         4526 t
ORIGIN      
        1 ttcaaccaag tcaatcaagg attcatctca tccgttgcgt ccaaaagaaa ccatatccct
       61 agaaaatcac taaattacca aacaccatta gaagtttttt tgagttacgt aaatggaaag
      121 ttttgtctcg cttaatttga caaataatat tctcaagaaa cacaaactaa tccgaagaat
      181 tctttgtttt gtcatttaaa tgtaacatag ccgaaaacga aaagtaatat tgatttattc
      241 ttctaacaca taaagaacag ctgtctccaa cttgcagaca gctgttctta tcagtcaaac
      301 aattgcttgg atctatgata gttgattgta cgtattcacg acattttcta ctgtaaatcc
      361 aaattcttct aaaattttag tgccaggggc agatgcaccg aaatgatcga ttgtaatcgt
      421 tgcgccctct gttcctacgt agcgttccca tccaaatgat gcagcagctt cgatagccac
      481 acgtttcttc actgatttag gaagtacact ttctttatat tcagaagatt gtttttcaaa
      541 gaggtcaaag cttggcatag aaacaacaga aacatctttg cctttttcag ccaattcttt
      601 ttgagctttc acagccaaat caacttcaga acctgttgca atcaaaatac cttctggtgt
      661 ttctccttgt gatggagaaa gaacatacgc gccttttttc accatttcat ctgctacttc
      721 ttttgtagaa ggcaatactg gaaggttttg acgactcaat acaaggatag taggcgcatc
      781 tgttgtctcc atcgctactt tccaagcagc acgtgtttca tttccgtctg ccggacgaat
      841 cacttgaaca ccaggcatgc agcgaacaga tgctagttgt tcaatcggtt cgtgcgttgg
      901 tccgtcttct ccgacagcaa ccgagtcatg tgttaagacg taaacaacag gagtatgttg
      961 gattgctgct aaacgaactg ctggtcttag ataatcgaca aagacaaaga atgttccacc
     1021 gtagatacga gttccgccat gaagttggat accattcata gccgaagcca ttgcaaattc
     1081 gcggacaccg aaccagatgt tgcgtccttc ataatgttca ggagtgaaat ctttatctgc
     1141 agccaccatt gtattattgg aacctgacaa gtccgctgaa ccaccccaga agcttggtat
     1201 agctttcgat aattcttgga tgacttcttt acttgaaaca cggcttgctt gactgcttcc
     1261 aacttcataa gttggcaatt ctgcatccca gttttctggt agtttgccat caaaagcatc
     1321 ttcaaattgt tgtgctaatt caggataagc ttttttataa ttggcaaaca tttcattcca
     1381 tgcatcttca gctttttgtc cttcttctat catcgtttga tggaagcggg cagctacttc
     1441 ttctggaaca gtgaaatctg gatactccca accataaact tctttagcca tcttgatgcc
     1501 atcttcacca agaggagcac catgaacagc agatgttcct tctttaggag caccatagcc
     1561 gatgactgtt tttacttcga tcaatgttgg tttgtctgtt tcagcttttg cttcttcaat
     1621 cgctttagag attgcttcta ggtcatttcc gtctttcact aagatatgtt gccagccata
     1681 tgcttcatag cgagcaccaa cgttttctgt gaaagctttt gaagttggac cgtctagtga
     1741 aatatcgttt gaatcgtata aaacgatcaa tttccctagt ttcatatgac ctgccatcga
     1801 acttgcttct tgagataccc cttccatcaa gtctccgtca ccgcatattg catacgtata
     1861 gtgatccatc acattaaagt tttctttgtt atacgttgct gcaaggtgcg cttctgccat
     1921 tgccataccg acagccattg caatcccttg tcctaatggc cctgtagtgg cttctacacc
     1981 atctgtatga tgtacttcag gatgtcctgg tgttttagaa tcccattgac ggaattgttt
     2041 caaatcatcg attgtcactt gatagccagc taaatgaagc aagctataca acattgctga
     2101 cccatgtcct gccgaaagaa cgaaacggtc gcggtcagcc cagttttttg atgtcgttgg
     2161 atttactttc aggtgttttg tccataacgc atatgccata ggtgctgcgc ccatcggcaa
     2221 tccaggatgt cctgagtttg ctttttggac tgcttcaata cttaatgtac gaattgtgtt
     2281 aacgccaagt tggtcaattt tgtcaaacaa aataacccct cctattaatt taggtttttt
     2341 tacctttatt tcttacactt tcattctata agatgtcaca cgaaaaagca atctttttca
     2401 gtatttttta ctaaaagttg tcatttcggt tgtgtaagcc ttttttcttt tggatttctt
     2461 ttaatttttc cggggtcacg tcattgccct caggatcgac gactttcatt ccttcgatat
     2521 gatgacgcat accggaacga aaagcttcca aatactcttt gcgcaagttg atttgttctt
     2581 gttcttcggc ttttgagaga ccattttcct tctttttacg tgcaagttca ttgatccgct
     2641 ctattttttc aggtgacagc atgctcagcc ctccttttac tctccttcat attatataaa
     2701 aactgaaaaa aaacaactag ataaactaaa taaaatacga acattcgttt gatttagcgc
     2761 tttttatatg gtactattta tttatcaagt gaaagaaggt gtaccttgtg gcccgacaaa
     2821 ctgaatcaag acaaatcgaa gtcttacgat atatccatga acaagtaacc caaaaaggat
     2881 acccgccgac tgtccgtgaa atcggggaag ctgttcagct ttcttctact tctactgttc
     2941 acggtcatct tgcacggctt gaaaaaaaag ggtttatcca aagagatccg acgaaacctc
     3001 gtgcaatcga gttgacaaaa caaggtttag aaaagattgg gattcgtcca acaactattc
     3061 cagtacttgg cgtcgtaaca gctggagaac ccattttagc agtagaggat gcttcagact
     3121 tctttccaat ccctcctaat ctttcttccg aagaaggcag tctctttatg ctgacaattc
     3181 gtggagatag tatgatcaat gctgggatat tggatggtga caacgtgatc gttcggaagc
     3241 aagaaactgc tcaaaatggc gatattgtca ttgcaatgac tgccgaggac gaagcgactt
     3301 gcaaacgatt ctataaagaa aaaaactttt tccgtctgca gcctgaaaac gacttgctcg
     3361 aaccaatcat cttagatcaa gtatctatat taggacgagt agtaggactt tatcgtagtc
     3421 atatctatta attgctacta attaaaagaa aactttttaa aaagagtttg agacgttggt
     3481 ctcaaactct tttttcgccc gggttcttct tctttcaaat ttttatcact tccctcttta
     3541 caaaccgaac aaatattcgt atactaaata tacgaacaat tgttcgcata aattaaagag
     3601 gtgagaattc atgaaatcaa tgagacgtct tggcttagta ttaactgttt tgtttttagg
     3661 tattattatt ggtaatttcg gcatacgtcc agctttactt gtgtttgttc cgttatttac
     3721 tttgtggttc atgttgtggg atgaaaagaa gtaccgtcaa tctgagcggc gcagaattca
     3781 tgaagatcat ttctatcatg agccctacta cagatccagg caatcataat aaggattgac
     3841 tgctaattat tcaaaagaca agaagttaaa cttttacggg ggtgtgacaa aaatcggtca
     3901 catccttttt ttcgaataaa cggtgttcta aagccagttt ttcggtatta agtcaaattg
     3961 cggaaaaagt gattttttaa acttctactg gtctttgctt gtttccattt ataaaaaaat
     4021 agcgaccctc aaaagtcaga tagagaatct gacttttgag ggtcgcttta aaatcgcttc
     4081 tttaagaata acatcaagaa tagcttcttt tttctgctta atattccatt aatgtaatga
     4141 tgtcatatcc tttgattttg tcgcgtccat ggagatccat caattcgatc aaaaatgcgc
     4201 agcctacgac tacaccgcct aattgttcga tcagttcgat cgtcgctttg attgttcctc
     4261 cagtagctag caaatcgtca caaatcagca cacgttgacc tggtttaatg gcatccttat
     4321 gcaatgtcaa tgtatctgtt ccatattcta atccataagt cacttcgatg gtttctcgag
     4381 gtaacttccc ttttttgcgg actggggcaa aaccgactcc taactcataa gcaacaggac
     4441 atcctacgat gaatccacgg gcttctggcc caactaccat atcaattcgt ttttcttttg
     4501 cataatttac gatttgttta gtagcttccc gataagcgtc accatctgcc atcagtgggg
     4561 aaatatctcg aaacacaatt ccttttgacg gatagtcggg aatactggca atataatctt
     4621 ttaaatccac tctttgtttc ctcctaggtt gttaaccatt tctttatggt aggaatatcg
     4681 ctcaaaagca aaaattcctc aactttgatt ttattgagcc gtcgctggta taatctgctc
     4741 tcagtcaaag gatgactttc tgggctagcg actttttgta aaacaccgtt ctctattgta
     4801 acaaatttca attcaaaaaa cacctgtatc ataaaaatta ataatttttc agggatacgc
     4861 aaatattgag ctattgcttt tattttatga cgtatatcaa ttcgttcttg agaatggatc
     4921 aatttaaaca attttgcgta ctgttctctt gtgcctaccc cattcaaata ggcctcatca
     4981 ggtgaaatcc ccatcagata aacacgattg aatgaataag accgaaaaac ttctttcaga
     5041 tccgctaaat catctggaca atccataaca actagagagt ttggttttct tttttcaatt
     5101 attgccttta cagaatctag tccttcgaaa acaatcgcag cttcctggat aatctcaggc
     5161 acgaatttca acgtcgaaga atcaaatgct aaataaaggc taccttccga cgttcggctc
     5221 tgttcacgga aacgttttgc ccgccaatca aatacttgca gaccacccac agaaaaatca
     5281 gataccatca gctgcggttt cttacgcccg ttccattcat tgatcgaaag ctttcctacc
     5341 acatctacct gattattcag cagttcttct tcttgagtac caaaaccaaa agcgaccgca
     5401 tcaagctgac tcgatgcatc ggaaaggaca aatttcagat gctgttggtt tgctccgatt
     5461 cttttgatat tttctgcttg gacttgtcga aataaaaact gaggcaaagg attatccgta
     5521 ccaaatggcg ccaatagttt cagctcatca attcgctcta ctgttacttc atttggcagc
     5581 aatacttcat caattctcaa ctcaggccct ctcatcaagt caatttgatg atcgactata
     5641 tattggttca tcttctcctg tagtatagtg acattttcac ttggcatcgt taaaccgact
     5701 gctgcatgat gtcctccgaa gtaagtaaat aaatctcgca tctggtcaag catttcaaaa
     5761 agattcaagg cttcaatact acgtccagat cctttagctg atccatcttc ttttaaagta
     5821 aggacaagcg ttggtttacc cgtttcattc attattttcc cagcaacaat gcccaaaacg
     5881 ccttcatgcc atcctcggtt taccaataga tggatatgat tctccttatt gaccatcgct
     5941 aatgcttctt gtgttatctg ctctacgatt gcttttcttt cttcattgat ctcattcaat
     6001 ttttttgctt gaacggatgc ttcctcttca tcaaacgtcg caagtaacga gacggcagga
     6061 cgtggatcgc ccatccgacc gattgcattt agtctgggag caatcgaaaa accgatcgtt
     6121 gtttcgtctg cgtccctcat ttttacgccg ctttcttcaa atagcgcctg aagtcctatc
     6181 cgttctgaat gatgaatggc atcgattcct aatgaaacaa ggattcgatt ctcatcagtc
     6241 aatgaaacta aatcggcgat cgttcctatc gctactaaat ccaaaaattc tgcaggcggt
     6301 tcttccaata atgcagaagc tactttgaat gctactccga ctcctgccaa atctggaaat
     6361 ggatagtgcc ccgctggatg acgagggtga acaatcgcat aagcgtctgg taaattctca
     6421 ggtagttcgt gatgatctgt aatgatcacg tctacaccag cttgctgagc gtatgctacc
     6481 gcatcatttc ctgctacccc gttatcaaca gtgatgatca actggattcc ttcttcgatc
     6541 ttttcttggt ataccgattg gtttggtcca tacccatgct caaatcgatt tggcaggaat
     6601 acttctactt ctgcaccaag taattccagt gtttctttca ttaccgttgc actggtgatc
     6661 ccatctgcgt cataatcacc gtaaacgagg attttttctc cactgattac agcttcttgg
     6721 attcggctga ttgctttatc catatcgtgt atcagatacg gatcatgtaa ttcttgttcc
     6781 tttggatgta aaaatcggtt gatatccttt tcctcatggt aacctctttg ccacagtaac
     6841 ttaccgacaa atggagataa tttcagttca cggatcattt ggagaaagga ttcagaaggc
     6901 tctgtctcgc ttaacttcca atcaaaattt gcttgtctca ctttctcact cctaacttag
     6961 ttattatagc aaaggaaatc agcaaaaaaa agcagaaaga aagaataaaa acagatcaga
     7021 tccgctagac agatgttcag caggacctga tcttttgtac gaccatttat gaaatatccc
     7081 tattagtcaa aataattgaa gctttgtttg ggcgattgag ttttttgttc ttccggcgct
     7141 ttcaattgac tgatttgtgc ggttttcgct tgtaactccg cctgataagc agcttccaac
     7201 tccgcacgtt cgtttctaaa ctcgcgttgc tgttcttctt ttgcttcttc aattttcttt
     7261 tgaagttctg tttgatattc tgacatttcc tgagtcagct gtttgatttg ctttttttgc
     7321 tgccatagtg ttgccgaagc agtcaaaaat acaatcagtg caccaatcag cgcagaaccg
     7381 atgatcacta agatcaatgg cgcagaaaag gaagtgaagc caaaacttac aggaacattt
     7441 ttattgttca atacagcaaa aagaacaata atcaaaacta aaacaaatcc aagaatcaca
     7501 ttgctttgct ttttcaattc cttcacctac tttttgttaa aaattccgct ggctaaataa
     7561 tcaccaatcg ttggaaacag cgtataaaat tttgatgcaa cttctaaaat aaacggttga
     7621 ttgatttccc gttttggatg tctcatattg cggacgatca tttttgccaa cttggcaggt
     7681 tctaaaacga tttgtccgac tttttccaaa tagcttccgc ttggatcagc cttatcgaag
     7741 aaatttgtct cgattggacc tggattcact gtcgtaacag ctacgccaag tggtttcaat
     7801 tctaatcgca aagcgttgga gaagccaagg actgcaaatt tcgtggcaga ataaaccgtt
     7861 gattttgcag ttgccatttt tccagccata gaggcgatat taatgatatg accttgtctt
     7921 ctttctgcca tatcgatcgc aaatttttgt gtcaaaacca tcattcctaa gatgttgact
     7981 tcaaacatat cgtatgcttt tccaaggtcg aatgtaagga agtcttcgaa aatcccaaaa
     8041 cccgcattgt ttaccagaat atcgacgcgc ccgacttctt ccatcacctt ttcatataat
     8101 tcgtccacgc tttcaggatt gctgacgtct accttgaaag cataagcctc tttgccgctc
     8161 aagcgtctgc actcgtctct gacaccttct acaaatgcca ttcttcgtgc gcaacttata
     8221 acgattgctc cttgctttgc tgcttcataa cagatttgtg cgcccagacc gcctgaacta
     8281 cctgttacta aaacaacttt atcttctaaa ttcatgcctc ttcctcctcg cgaaatggaa
     8341 tctcaatgct atcaaaatct ttcataatcg tcgtgttttc aaacacttct tgtgcttctt
     8401 tttcaagctc taaagctgct tttcctaaat atctcgcact gatatgagta agtagcagcc
     8461 gttttgcctg cgcttctttt gcaacttcag cagcttgatg agtcgtggaa tggaaatagt
     8521 tgtgcgccat tcttgcttca tctttattaa atgtgctttc atgtaccaga atatcgctat
     8581 tttccgcaag cacaacggaa ttatgtgttt ttctcgtatc gcctaaaata gtcacgattc
     8641 gtcctttttt atctggacct acgaaatctt ttccattgat cgtccgccca tcttctagtt
     8701 ggatcgtttc accttgcttc agtttgccgt acagtggtcc agctggaata cctaattctt
     8761 ttagacgatc cacctgcaat tctcctttat gatcatgttc aaccacacga taaccaaaac
     8821 tgtcgatccc atgatttagt ttttttgtat aaacagaaaa ttgctgatca gtaaaaatcg
     8881 gggctgtttc atttagctca atgaatttca gcggataact taaacgcgtt tgcgaaatac
     8941 ctaaagagac ttttatgtat tcctctatcc cttttggccc gtaaatctct aatggcgtat
     9001 ccccaccttg aaatgaccgg ctgctgatga gcccgggaag tccaaaaata tgatcgccgt
     9061 gtaaatgtgt aatgaaaatt ttaccgattt ttctcggacg aatagtcgtt cgcaaaatct
     9121 gcatttgcgt gccttcgcca cagtcgaaca accaaacttc attgcgctcg tctaaaagtt
     9181 tcaatgctat acttgtcaca tttctatgtt ttgcaggaac acctgcacct gttcctaaaa
     9241 attgtaattc cataatctac ctgactttct ttaagttcct ctttaatttt agggtaaaaa
     9301 gaagggataa tcaaccgtaa gtcgagaatg ggttcaaatt aaaaataacg ttctaaagcg
     9361 atagcgtttt ctttgaaaat tactataaaa acagcagcga acccgtttga gatcctctgc
     9421 tgttttttat taatttgaag atccagaact ttcattgtcg ttggaagttt gcggatgttt
     9481 gtccttccaa gtcttttgtg cagcttcttt cgcatgattt tcagcttctt tcgcatcatg
     9541 cacatctttt tcgacatctt cataatccaa gcgagtaatt tctccgccta attccatcat
     9601 taccaattga ttcagtgggc gattgtcctc ttcatcagca atcaagatca gtcctgtagc
     9661 tccttcaccg attttatttc ctacaaagtc gaacacggtc tgggcatctt ttatttcttt
     9721 ggcatcttgt gtcgcaccga tcattcctcc ggcaaaccat cctaataaaa tccctagcgg
     9781 accacctaat acaccaacca acattccgat taatccaccc gtagaagatt tgttattacc
     9841 ggtaaagtcg ataaaatctt ctattttgaa ttgatgtgtc ccattaggat catggctgac
     9901 aactgccatc tgttctcctt taatcgaacg aacagcatgt aactttttga tttctgaaaa
     9961 tgcctgatat gcctggcttt ctacttgaaa atttaaaatg attactcgtt tgctcattaa
    10021 aaacgcccct ttcatacctt cttgttattt attttacctt taattgaaaa ataagaaaag
    10081 aaaagcactc tttttttcca ataaaatagg gctgtgacaa aagtcgtatc acagctcctt
    10141 tttccgaata aacggcgttc ttgaagctga tcacttctgt cacaacctcc cttaaatctg
    10201 aatctatatt ttttggtatt tgtttttatt tgcttgccta ttcgacaaat tcaaattcaa
    10261 actcaccgat acgtacaagg tcgccatctt tcgcaccgcg tgcccgaaga gcttcatcca
    10321 cacccatacc acgtaattga cgagcaaaac gcatcactgt ttcatcatga tcgaaattcg
    10381 tcatttggaa taatttttcg atcttctcac cagataagat ccacgttgca tctgaatcac
    10441 gatcgatttg gaactcagga ccttctggat tgaatccata gtgtacagtt tcttcttcca
    10501 gttcttcttc gtacaatggg aattctggcg ttacttctaa caaatctgct gtagcattca
    10561 ataacgcgtc caatccttgt cgagtcactc cagaaattgg aaatactggg atgtcatcag
    10621 caaattcatc ttctttttcc ttgttcagct gttctttgaa tttcactaga ttttccggtg
    10681 catccggcat atccattttg tttgccacga tgatttgcgg acgctctagc aagcgtaagt
    10741 tataagtaga taattctttg ttgatggcaa gatagtcttc ataaggatct cgtccttcca
    10801 tcccactcat atcgatcaca tgtaaaatta ctcgtgttct ttcgatatga cgaaggaatt
    10861 gtgtacccag cccaacacct tgcgaagctc cttcaatcaa gcctggcaag tctgctgcgg
    10921 caaaacttct gccatcactt gtagtaacca ttcctagatt aggtacgagc gtcgtgaaat
    10981 gataagcgcc gatttttgga cgagcagatg aaataacgga tagtaaagtc gatttcccga
    11041 cagatggaaa acctacaagt ccaacgtctg ccaatacttt caattctagt tcgatttttc
    11101 gctcttgtcc tggttcaccg ttttcagcaa tttccggtgc aggattccga ggagaggcaa
    11161 aacggatatt ccctcgtcca ccgcgtccac cttttgccac aactagtgtt tgaccatttt
    11221 caattaaatc tcctaaaaga gcaccagtct ccgcatctcg gacagtggtt ccttgtggta
    11281 ctttaacata ggtatcttct gaaccacgac cgtgcatccc tttactcatc ccattttcac
    11341 ctggctgcgc tttgaaatgg cgattgaacc gaaaatccat caatgtacgt aacccttcat
    11401 cgacgatcaa gatgacatcg ccgccacgac caccgtctcc gccagccggt ccgccgtcag
    11461 gaacgtattt ttcgcgacga aatgcaacca ttccgtctcc gcccttgcca gctttcacat
    11521 cgatcgttac ttgatctaaa aacatggaca taattaagtc ctcctattat tcatttattt
    11581 atttgactgc ctgaatttcc acaataaaca aaagtcttac ttattttatc ggtgaaatct
    11641 gattttgtct atcatttcag caaaaagatt gtttaccttc ttcttttttt atcatttggt
    11701 aaggtttcca accttttttc tgctaccaag tataattttt tgacaaaata ttctttgcgt
    11761 agcagaataa cagtgtatta attgtattaa ttgaggatgc cgtttcacag cacccctaat
    11821 aaaggaggaa cttttgatat gacagtaaaa gtaggtatta acggatttgg gcgtattggc
    11881 cgtctggctt tccgccgcat caaagaagtc tcagatgata ttgaagtagt agcaatcaac
    11941 gacttaacaa gtccaactat gttagcacaa ttattgcaat ttgactctac tcatggtact
    12001 tatcctggca cagttactgc aacagaagac tcaatcgtag tggatggcga agcaactcgt
    12061 gtttatgcag aacccgacgc aagcaaaatc ccttgggcaa aagaaaacgg tgtagatatc
    12121 gtactagaat gtactggttt ttatacatct gaagaaaaag caaaagcgca tttagatgct
    12181 ggtgtcaaac gagtagttat ttctgcccca gctggtgcta tgaaaaccat cgtctataac
    12241 gtcaatgatg atacattaga tgaaaacgac aaaatcatct cagcaggttc atgtacaaca
    12301 aactgtttag cacctatggc ttatttctta aataatgaat ttgggatcga agttggtaca
    12361 atgacaacag ttcatgccta cacttccaca caaatgctct tagatggacc tgtaaaaggc
    12421 ggtaatttgc gtgctgctcg ttcagctgct gacaatacta tcccacattc tactggtgct
    12481 gcgaaagcaa tcggcttagt tatccctgag ctacaaggaa aattacaagg acacgcacaa
    12541 cgtgttccag tcgttgatgg ttcattaact gaattggttt cagtattgaa aacaaaagta
    12601 actgctgatc aagtaaacga agcaatcaaa aaacacacag ttgacaatcc ttcattcggg
    12661 tatgatgacc gtcaaatcgt ttctagcgat gtcattggaa caactcaagg ttcgatcttt
    12721 gacccaacgc aaacagaagt aacaactgct ggtgatttcc aacttgttaa aactgttgca
    12781 tggtatgaca atgagtatgg atttacttgc caaatgattc gcttactaga aaaattcgct
    12841 aacttgtaat ttgatagatc aatcgataaa agaagccttt gactggatcc agtcaaaggc
    12901 ttctttcttt tcatccccta cttttaaact cgttcaatga acatattggc ttaaaaattg
    12961 ttggactact tgctgatatt tttcaggatc tgtcttgtat gcttccgcat gctcggctcc
    13021 ttttacaaca tacttttctt ttggtccctt cgttgcacga tagaccttat ctaacatttc
    13081 aaatggtaca aatgtatctt cgtctccatg tataaagagc attgggcgct ggtttttctt
    13141 caattgttcc actgcatttg cttcaccgaa aaagtagcca gcacggactt ttgtcactag
    13201 actcgttact tgcataagcg gaaatgctgg aagactaaac atatcgtcta gctgataagc
    13261 aagctcatca tgaacggaag aataaccgca atcttcaatg attgctttca cattatctgg
    13321 tagtttttct ccgctagtca tcataactgt tgctgctccc atgctgacac cgtatagaac
    13381 gatttcttgc gattttccat tgttttctag cactttgttt atccactgta catagtcttt
    13441 acgctctggc catccaaatc caatgtaatc cccttcgctt tttccatgtc ctcttgcatc
    13501 aggaacaagg acattgtaac ccatatcatg aaacatcttt gcgtaattgg tcatcgtttc
    13561 agcgtttccc atatagccat gagccacaag agctgttttt tcacttttcg tttcagcagg
    13621 aagataaatc gctgataatt tcagaccgtc ttttgaagtg atggtccaat cgcttcggtt
    13681 ttcttttgaa ctaaaccatt tatgtgcatc cgtttctttg gatgtctgtg ttgtaccagg
    13741 tgtatcttcg tctaaaaagt ctttctcaga aggcacaaca gcataattat aaaaataatt
    13801 ccctgcaaac actaacgcaa caatcagagc taaagcgacg cctaaaagga ttttcatcca
    13861 aagtttcata aatcatccat ctcatttctc ttttttcata taaccttgaa ttagtataat
    13921 caaagaatct ctactatgca atgttcattt ttataacccg cgaattgtaa aattaagtta
    13981 gaaaaataaa aaggcattta
//
LOCUS       DACDYI010000076        13917 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.076, whole genome shotgun sequence.
ACCESSION   DACDYI010000076 DACDYI010000000
VERSION     DACDYI010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.076"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            131..505
                     /locus_tag="IWM52_002338"
     CDS             131..505
                     /locus_tag="IWM52_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9974564.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="IWM52_002339"
     CDS             complement(619..1248)
                     /locus_tag="IWM52_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9974565.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="IWM52_002340"
     tRNA            complement(1382..1454)
                     /locus_tag="IWM52_002340"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="IWM52_002341"
     tRNA            1668..1741
                     /locus_tag="IWM52_002341"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            1696..1845
                     /locus_tag="IWM52_002342"
     CDS             1696..1845
                     /locus_tag="IWM52_002342"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974566.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            2338..3666
                     /locus_tag="IWM52_002343"
     CDS             2338..3666
                     /locus_tag="IWM52_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP9974567.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="IWM52_002344"
     CDS             3868..4701
                     /locus_tag="IWM52_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAP9974568.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="IWM52_002345"
     CDS             4717..5454
                     /locus_tag="IWM52_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAP9974569.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="IWM52_002346"
     CDS             complement(5609..6577)
                     /locus_tag="IWM52_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAP9974570.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELKKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="IWM52_002347"
     CDS             6809..7642
                     /locus_tag="IWM52_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAP9974571.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="IWM52_002348"
     CDS             7676..8515
                     /locus_tag="IWM52_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9974572.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="IWM52_002349"
     CDS             8541..9101
                     /locus_tag="IWM52_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9974573.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="IWM52_002350"
     CDS             9300..11021
                     /locus_tag="IWM52_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAP9974574.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="IWM52_002351"
     CDS             11186..12328
                     /locus_tag="IWM52_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP9974575.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="IWM52_002352"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="IWM52_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAP9974576.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4751 a         2297 c         2833 g         4036 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc atttttttta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttg
//
LOCUS       DACDYI010000077        13747 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.077, whole genome shotgun sequence.
ACCESSION   DACDYI010000077 DACDYI010000000
VERSION     DACDYI010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.077"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..863
                     /locus_tag="IWM52_002353"
     CDS             <1..863
                     /locus_tag="IWM52_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9974577.1"
                     /translation="MSQMMDFLSIFTDGMVAGSRILKIMDTEEYAPQQNPGADQMITS
                     GKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGSGKSSIINVLMRFYEFYEG
                     QILIDDHDIREYPMEELRKKMGMVLQDAFLFYGDIAGNIRMMNPAITDEQIRHAAEFV
                     QANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVTDPKILVLDEATANIDTET
                     EGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGHIVEHGNHETLLAQNGLYA
                     DMYKLQSSGI"
     gene            1189..2040
                     /locus_tag="IWM52_002354"
     CDS             1189..2040
                     /locus_tag="IWM52_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9974578.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEDLQVHLNDSVYIALTDHLY
                     TAIERQKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSIHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFSCIEKIADFLTGAFDYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            2137..4017
                     /locus_tag="IWM52_002355"
     CDS             2137..4017
                     /locus_tag="IWM52_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9974579.1"
                     /translation="MGKYQELAENIVKNIGGKENIRSLTHCVTRLRFKLKEENKAKDD
                     VLKKMEGIVTIMKSGGQYQVVIGNHVPDVYAEVLTVAGISDEAEEEVSSSSNLFNRLI
                     DILSGCFQPFLGALAAAGMVKGLNALFLFLKFYTEGSGTYIMLNGIGDAIFYFMPVIL
                     GYTAAKKFHLNPMVGLVIGAALCYPTVQGAALQSGFPDGSPAPYSLLGLPAYETFLGI
                     PWVGSSYTSSVVPVIFIIAFAGQVQKIAKKWIPAVVQTFLVPFSVLLIALPAGFLIIG
                     PIVSILTDLLSTGFQALMDFSPAVYGAVLGFLWQVLVIFGLHWSIIPLAIMQLAQNGW
                     SQMLVGVYGASFAQTAVVLAMYFKFKDQKMKALCMPAVISGIFGVTETAIYGITLPKK
                     KPFIYSMIGGAVSGIYLMASGTISYTMGGLGIFGVVNYITETDASGMFQAFIGIAIAS
                     VIGFSLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGRGI
                     LIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESLVQ
                     QGERVKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGETVGPDDALITA
                     LT"
     gene            complement(4057..4959)
                     /locus_tag="IWM52_002356"
     CDS             complement(4057..4959)
                     /locus_tag="IWM52_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9974580.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNDVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            5190..6593
                     /locus_tag="IWM52_002357"
     CDS             5190..6593
                     /locus_tag="IWM52_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP9974581.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            6593..8026
                     /gene="ascB"
                     /locus_tag="IWM52_002358"
     CDS             6593..8026
                     /gene="ascB"
                     /locus_tag="IWM52_002358"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP9974582.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDVFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQVKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            8171..9097
                     /gene="trxB"
                     /locus_tag="IWM52_002359"
     CDS             8171..9097
                     /gene="trxB"
                     /locus_tag="IWM52_002359"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAP9974583.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            9311..11038
                     /locus_tag="IWM52_002360"
     CDS             9311..11038
                     /locus_tag="IWM52_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP9974584.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEEAKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            11110..11403
                     /locus_tag="IWM52_002361"
     CDS             11110..11403
                     /locus_tag="IWM52_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP9974585.1"
                     /translation="MPDTQKSKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(11495..12355)
                     /locus_tag="IWM52_002362"
     CDS             complement(11495..12355)
                     /locus_tag="IWM52_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAP9974586.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLKQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            12447..12875
                     /locus_tag="IWM52_002363"
     CDS             12447..12875
                     /locus_tag="IWM52_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP9974587.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            13037..13213
                     /locus_tag="IWM52_002364"
     CDS             13037..13213
                     /locus_tag="IWM52_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAP9974588.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            13240..13686
                     /locus_tag="IWM52_002365"
     CDS             13240..13686
                     /locus_tag="IWM52_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAP9974589.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         4498 a         2349 c         3074 g         3826 t
ORIGIN      
        1 caatgtctca aatgatggac tttttgagca tcttcacaga tgggatggtt gctggtagtc
       61 gtatcttgaa gattatggat acagaagaat atgcaccaca acaaaaccct ggagcagatc
      121 aaatgattac ttcaggtaag attgagtttc gcaatgtaag cttttcttat gatggagagc
      181 acaatgtatt aaacaatatc tcttttgttg ctaatccggg agagaccgtt gcattagtgg
      241 gacacaccgg aagtgggaaa agctcgatca tcaatgtgtt gatgcggttt tacgaatttt
      301 atgaaggtca gatattgatt gatgatcatg acattcgaga atatccaatg gaagagttac
      361 gtaagaaaat ggggatggtc ttgcaagatg ctttcttgtt ttatggggat atcgcaggaa
      421 atattcggat gatgaatcca gctatcacag acgaacagat acgccatgcg gctgagtttg
      481 ttcaagcaaa caaattcatc gatgaattac caaaaggata tcactcaaaa gtgatcgaac
      541 atggtgccag ctattctagt ggacaacgac agctgatcac ttttgcgcga acaatcgtga
      601 ctgatcctaa aattctagta ttggatgaag caacagcaaa tatcgatact gaaacagaag
      661 gattgatcca agaaggattg gcgaaaatgc gacaaggacg gacaacgatt gcgattgccc
      721 atcggctgtc aacgattcga gatgcagaat tgattttagt tttagacaaa ggtcatatag
      781 tagaacacgg gaatcatgaa acacttctag cgcaaaatgg tttgtatgca gatatgtaca
      841 aactgcaaag cagtggtata taaaagatgt tgtgacagta gcattttgac ctaagcacta
      901 gagtgaaaat aaaacatagc ttcccatatt tttcatctgt gagagttagt gtcgtagtta
      961 ccagatttaa aaaagacgac gtcagctagt cagaagtgcc taatagcccc taagaaagcg
     1021 ttgacaaaat cgtttgcttt cgataataca aagaaaacga tacaacttgg atagtgattg
     1081 cctaatgcaa gctaaacctg atgagtctcc catggtttca tggacggctt tttgggtttt
     1141 tctttttgtt ttggatacag ccctatatat catggaaagg agcaaaacat gctgattgag
     1201 aaagtactga ataacaacgt cgtaataacc agagatgaaa gaaatcagga aatgatcgtg
     1261 atgggaaaag gtttggcatt caaaaagaaa gtgggtgacc cgatttttga ctcatgcatc
     1321 gacaaaaaat ttcagttgac aaatgacgga ctttcacaac agttccaaga attggcacaa
     1381 gcgattcctc ttgagtactt agaaatttct tgtgaaatca ttgaatatac aaaagaggat
     1441 ctacaagtcc atttgaatga ttctgtgtat attgcattga cggatcattt gtatacagct
     1501 atcgaacgtc aaaaaatggg gatctttgtg ccaaatgtat tgctgtatga tatcaaacaa
     1561 ctgtttccta aagagtatgc cattgggaaa aaaacagtga agaaaatctc ggataaatac
     1621 cacatcaagt tatcagaaga cgaagccgga tttatcgcat tgcatttagt caatgcacaa
     1681 ggcattcaaa gccagcaaga caatacctac ctgtttacca caacaataca ggatatttta
     1741 aatatcgtcc gctattacta taagattaca tttgatgaag attcaatcca tttttttcga
     1801 ttcgtcacac acctacgttt ttttctgaca cgcgtcctag atggagagaa atcagaagtg
     1861 ggagaagttt ctgaggaatt gttccaggtt attcaaaaaa aatataagga cacgtttagt
     1921 tgcattgaga aaatagcaga ctttttaacc ggtgctttcg actaccagat gtccacggat
     1981 gaaaaaatgt atttaacgat ccatactgcg cgtttagtgc atgagaatcg ttgtaaataa
     2041 ataaaaaagg aggacaaatt aacaagaacg tatgaaaata agggtagtga cattcagttg
     2101 gctaaacctt ccactaatta attggaggga atagaaatgg gaaaatacca ggaattagca
     2161 gaaaacatcg taaagaatat cggaggaaaa gaaaatatcc gtagcttgac acactgtgtg
     2221 acgcgacttc gtttcaaact aaaggaggaa aataaagcaa aggatgatgt tttgaaaaag
     2281 atggaaggga tagtcaccat catgaaaagc ggcgggcaat atcaagttgt aatcgggaat
     2341 catgtgccag atgtctatgc agaagtactg acagtagccg ggatttccga tgaagcagaa
     2401 gaagaggttt cttcaagtag taatctgttc aatcgattaa tcgacatatt aagtggctgt
     2461 ttccagcctt ttttaggtgc tttggctgca gcgggaatgg tcaaaggatt aaatgctttg
     2521 ttcttgtttc tcaaatttta tactgaagga tcgggtacgt acatcatgct gaatgggata
     2581 ggggatgcaa ttttttattt catgccagtg atcttagggt acacagcagc gaaaaaattt
     2641 catctgaatc cgatggtggg gctcgtgatt ggtgcagcac tttgttatcc gactgtacaa
     2701 ggagcagcac tacaaagtgg tttccctgat ggcagtcctg cgccttatag cttgctgggt
     2761 cttccggctt atgaaacatt tttgggtatc ccatgggtgg gcagcagtta taccagtagc
     2821 gtcgtacctg ttatcttcat tatcgcattt gccggacaag tccaaaaaat tgccaagaaa
     2881 tggattcctg cagtcgtcca gacctttttg gttccttttt ctgtgttatt aatcgcactt
     2941 cctgctggct ttttgattat cggtccgatt gtcagtatac tgacagatct attaagcacg
     3001 ggattccaag cattgatgga cttttctcca gctgtgtatg gtgcagttct agggtttctt
     3061 tggcaagtac tggtgatttt cggacttcat tggagtatta tccctcttgc gattatgcag
     3121 ctagcgcaaa atgggtggag tcagatgtta gtaggcgttt atggcgcgag ttttgcacag
     3181 acagcagtgg tattggcgat gtatttcaag ttcaaggatc aaaaaatgaa ggcgctttgc
     3241 atgccagctg tgatttcagg aatcttcggc gtaacagaaa cggctattta tggaatcacc
     3301 ttaccaaaga agaagccatt tatttactcg atgattggtg gtgctgtttc aggaatctat
     3361 ttgatggcat ctggaacaat cagctacaca atgggcgggt tagggatttt tggggtggta
     3421 aactacatca cggaaacaga cgccagtggt atgtttcaag cgtttatcgg cattgctatt
     3481 gcttcggtaa tcgggttttc gctgactttt ttcttttgga aggatgaagc aggtgaaaca
     3541 gatgatatac agaaaaaatc aggtaaagaa attgtaaaaa caccgataca aggacaaatc
     3601 gctccactaa atgcagcaaa ggatgcagct tttgcacaag gaacactagg tcgaggaatc
     3661 ttgatctacc ctgaaaaagg agaagtacgt gccccttttg acggaacagt catgaccctg
     3721 ttcccgacga aacatgcaat tggaatggtt tctgaaacag ggctggaatt gctgattcat
     3781 gtcggattgg atactgtgca gttggaaggg aaatattttg aaagcttagt gcagcaagga
     3841 gaaagagtga aacaaggcga tctgttgcta cgcttcgaca aaaagaaaat cgaaaaggaa
     3901 ggatacagtt tagaaacacc tgtgatcgtc accaattaca cggattattt ggatattttg
     3961 gaaaaccaag gggagacggt cgggccagac gatgcactta tcaccgcact gacctgaacg
     4021 gttaggtgaa gaatggacta ggcttaacta ttctttttat tccacttctt tattttcaaa
     4081 attgaaaggc tctttcatga tggctgagct gatagggcgt ttatcgaatt tttgaccgac
     4141 agaaattaga tggactaaat tctgttgata attttctgca aaaacacaac tatataacac
     4201 gtctagaaga taacaaattg atgtattgat cgtaaaatta ccaatttttg aatacagtcg
     4261 ctcgcgtgtc gtaagtcgca aagcggctgt actcattgca gatagcgttg aatcaccgat
     4321 actggtcaaa gctaaaattg gaacatcatt ttgtttcaat atttctgcgg tacgaaggat
     4381 cacattgttt tcacccgtat aagaaatcag aatcccgcac atatcactcg tgcaattgta
     4441 cgcttcatag aacgattcac caaaagtggt gacaatcgtg acatccattt tgatgcgctt
     4501 cattttcagc atgaaatctt gagaaatcaa agtattcgag ttactggcga aaatcacgat
     4561 tttttttgct tgaagcatta gttgtttggc tttttgcagt tggtcatgat gaatgagaga
     4621 aagtgtatca tgaatcgtca tctgttctaa agaagccatt ttatttgcga tcgtcataat
     4681 accgtctgtg gtggaaaaag gaagattcgc atctacgtct tgaaaattcc cttcaagata
     4741 ggctgtttca gctaaaaaag cattacgaaa actgacccag ccatcaaaac ccattttttt
     4801 tgctacacgg atgagtgtag acgggtggac atagcatcgc tcagcgatct cttggatggt
     4861 catttcgcca agtgctggag aatcgcttaa gagaaactgg atcacttcat tttctgaagg
     4921 agaaaaggag atttctttga ttttttcacg taataacatg tagttacctt cttcttattt
     4981 ttttacgtaa attataactt ttttgaaaat gttttgtaaa cggatttata ctatataact
     5041 taaaatagac tttttttgac aagatattta caaaaatcca ctagaaaaca gtaacttttg
     5101 taaatgttac tggaaagaaa cggatttcta ttgtttgttt tgtttacaaa aaagataatg
     5161 aaagtgtaaa aaagtaaagg agtgagaaca tggcaaaagg aatcaaaatc gctacgattg
     5221 gtggaggcag cagttacacc cctgagttga tggaaggatt tatcaaaaga tatgaggaat
     5281 taccgatacg agaaatctgg ctcgtagatg tagaagaagg aaaagaaaag cttgagatcg
     5341 ttagtcagat gtctcagcgt atgtgggatg cttctccgta tgaagtcaaa atctatgcaa
     5401 ctctggatcg ggaagaagca ttgaaagatg cggattttgt tacgactcag tttcgtgtcg
     5461 gtcaattaga tgcacgaatc aaagacgagc ggatccctag ttattatggg atgattgggc
     5521 aagaaacaaa tggtgctggc gggatattca aagcttttcg taccgttcca gtggtcttgg
     5581 caatcgttga agacatgaag cgtttatgtc caaatgcttg gctgatcaat ttcgcgaacc
     5641 caagtgggat ggtgacagaa gcagttatca gatatggcaa atgggataaa gtgattggat
     5701 tatgcaatgt gccagtaatg gcgatgatga cagaaccagc aatgcttggc aagaaaaaag
     5761 aagagctgat ctacaagttt gccggtctaa atcatttcca ttggcacaaa gtagcagata
     5821 ataaaggaaa tgaccgcacg gcagaattga ttgatcgttt atacgatgaa gacaatggat
     5881 tgccgaaaaa tatctttgat atcccgtttt tcaaagaaca attaaaacaa atgaatatga
     5941 tcccttgtgg ctaccaccgc tattactacc gtgaagaaga aatgctggaa catgctttag
     6001 aagaatatca aacgattggt acaagagcgc agcaagtgaa agaaacagaa gcagagctat
     6061 ttgaattata caaagatcca gaattagacc ataaacctga acaattgcaa aaacgaggtg
     6121 gcgcttatta ttcagacgct gcctgtgaaa cgattgcttc aatctatgga aataaagaaa
     6181 cacagatcgt ggtatctaca aagaatgatg gagctgttcc tgacctgccg gcagattgtg
     6241 tagtagaagt cactgcctat gttggcgcac aaggagcgag aaatgtagcg tttggttctc
     6301 ttcctgctgc tgaaagaggc tggttgcaag taatgaagaa tatggaatta ctgacgatcg
     6361 aagcagctgt gactggtgat tatgggacag ctttacaggc gtttacgatc aatccgctta
     6421 tacgatcagg aaagacagca aaacggatca tggatgagtt gtttattgca caccagcagt
     6481 atttaccgca attcaaggaa acaatcagcc gattaaaaga agaaggaatc gaagttcaag
     6541 atgaactggc aagagaatta tcaggtaata cacatataat ggaggaagta taatgggatt
     6601 cagaaaagat tttttatggg gcggtgctac agcagccaat caatgtgagg gcggttatga
     6661 tgaaggtgga cgcggactag caaacgtaga tgttgtgcca atcggaaaag accgttttcc
     6721 tgttatcacc ggaaagaaaa aaatgtttga atttgatgag gatcattttt atccagcaaa
     6781 aattgcaatc gatatgtatc atcgttataa agaagatatt gctttgtttg cagaaatggg
     6841 gttcaagact tatcgaatgt ccatcgcctg gagtcgtatt tttccaatgg gggatgaaaa
     6901 agagccgaat gaagctggct tgaaatttta tgaggatgtc tttaaagaat gccaaaagca
     6961 cggaatcgag ccacttgtga cgattacgca ttttgattgt ccaatgcatt tgatcaaaga
     7021 atacggtgcg tggcgttcca gagaactcgt tggtttttat gaaaatctgt gtcgagtcat
     7081 cttcaatcga tataaaggac ttgtcaaata ctggctgaca ttcaatgaga tcaatatgat
     7141 ccttcatgca ccattcatgg gagcaggact gtactttgaa gaaggagaaa acgaagaaca
     7201 agtcaaatat caagcggcac atcatgagtt ggttgcaagt gccatcgcaa cgaagattgc
     7261 tcatgaagta gatccggaga acaaagtagg ctgtatgctg gcagcaggag catattatcc
     7321 atacacttgc aatcctgaag atgtttgggc ttctagaaaa tcagatcgag aaaattattt
     7381 cttcattgat gttcagtcac gaggagaata cccagcttat gcattgaaag aaatggaacg
     7441 aaatggcatt cagatcgaaa tggaaccagg ggatgccgca ttgctgaaag aacatactgt
     7501 tgattttatc tctttttctt attattcttc ccgagtttcg actaccgaca acgaactctt
     7561 ggaacagact gcaggaaata tctttgcttc tgtcaaaaat ccatatctgc aagcaagtga
     7621 atgggggtgg caaattgatc cattaggctt gagaataacg atgaatgata tttatgatcg
     7681 ttatcaaaaa ccattattta ttgtagaaaa cggcttaggt gcagtcgata cgccagatga
     7741 gaatggttat gtggaagatg attaccgaat cgattacctg gcagcgcata tccaggcgat
     7801 gaaggatgca gtagaactag acggagtcga tctattaggc tatacgactt ggggatgtat
     7861 tgatttagta tcagctggta ccggtgagat gaagaaaaga tacggattca tttatgtcga
     7921 tcgtgataac caaggaaatg gcacattagc ccgttcaaag aaaaaatcat tcaactggta
     7981 taaaaaagta attgattcaa atggagaaaa tctttcgaac gattgatcaa tgatttataa
     8041 aagtctattg tttttcagcg gatttttcga gtataataaa gctattcgag aaacgcaagc
     8101 agaacaatag actttttttc gcattggctg taaagtatgc ttacttgatt aagtaaagga
     8161 gaatctacat atgtatgatg taatcgttat tggcgcaggt cctgctggga tgactgctgc
     8221 tttatatgct tcacgttcaa acttatccgt attgatgatc gaacaaggtg cccctggcgg
     8281 ccaaatgaac aatacggcag aaatcgaaaa ttatcctgga ttcgattcga tcatgggccc
     8341 cgatctagct tataagatgt atgaaagtgt ctcaagattt gggacagaaa atgcttatgg
     8401 aatcgttcaa gatatcaagg atcacggcag ccataaagaa gtcatctgtg aagataaaag
     8461 ttacgaagga aaaacagtca ttatcgcaac aggctgtatc caccgtaaat taggcgtacc
     8521 tggcgaggaa gaatttgctg gacgaggcgt ttcttactgt gcagtctgtg atggtgcgtt
     8581 tttccgtaac aaacgtctag tagtcatcgg gggtggggac tcagcagtag aagaagccat
     8641 ctatttgaca caatttgctt ctgaagtagt gatcgtccac cgtagagatg aattacgtgc
     8701 acaaaagatc atccaagacc gtgcatttgc aaatgaaaaa atttccttta tgtgggatac
     8761 agtcgttgac gaaatcgttg gaaacgaaat ggtagtgaca ggtgtgaaag cacgtaatgt
     8821 caaaaccggc aatgaatttg atttagcagc agacggagta ttcatctatg ttggactgga
     8881 tccattaact gcacctttta gagaagcagg tttgacgaac gaagctggct ggattccaac
     8941 agacaacgaa atgaaaacga atatgccagg cgtttttgcc atcggagatg ttcgtgaaaa
     9001 agatttgcga caaatcacta cagctgttgg tgacggcggt attgctggac aacaagtgta
     9061 taaatacttg gaatctttag aagaaacaac tgcataagtt taaaaagagt agaagaacgg
     9121 gttcaattgt tcttttactc ttttttttcg cgtgaaattt actgaatctg atttatgcga
     9181 acacgtccaa caccttttga taaaagaatc ctttcttgat ttaagcgttt tttggtcctc
     9241 atactttcat atctaataag tgaatgctat acttattaag aaccatattt cagaaagagg
     9301 tgttattttc atgtcttggg aacaagtgta tgaacaatgg gtacatgaag aaaatttaga
     9361 agaaaactta aaaaaacaat taaatgatct gggtgaagat cctgaaaaac ttgaagatgc
     9421 tttttatgca ccgttggaat tcggtactgc aggcatgcgg ggaatcttag gacctgggat
     9481 caatcgaatg aacatctata cggtaagaca agcaacagaa ggactagcac gtttcatgga
     9541 tacacaaggt gaagaagcaa aacgccgagg tgtcgctatt gcttatgact cacgccatca
     9601 gtcaccagaa tttgcaatgg aagcagccaa aacactggca catcacaata tcccgtctta
     9661 tgtattcgaa agtctgcgtc caacaccaga gctttctttt gcagttcgtc atttgaagac
     9721 gtttgcggga atcatggtaa ctgcatctca caacccagcg gcttacaatg gatataaagt
     9781 ctacggagaa gatggcggac aaatgcctcc tgcagacgca gatgcattga cgaaatatgt
     9841 ccgtgaagtc agcaatccgc taaaagtcga agtattatct gacgaagaag ctaaacatag
     9901 cgggttgatc acgatcatcg gagaagaagt ggacgctgct tatctagaag aaatcaaagc
     9961 agtgacgatt gatcgtgaat tggtagaaac aatggggaaa gatttgaaac ttgtgtacac
    10021 gccacttcat ggaacaggga aaatgttggg cgaacgtgcg ttgaaacaag ctggattcga
    10081 acaattcgta ctagttcccg aacaagctgt ggcagatcca gatttcacta cggtcaaatc
    10141 gccaaatcca gaagaacatt cagcttttga atatgctata cgacttggag aaaaagaagg
    10201 agctgacttg ctgattgcaa cagatcctga tgcagaccgt ttaggcgcag cggttcgttt
    10261 gccagatggt tcttatgaag tattgactgg gaaccaaatc ggggcattgt ttgctcaata
    10321 tattttggaa gcacatcaat caagaggcac tttaccagaa aacgcagcgg tattgaaatc
    10381 aatcgtttcc agcgaattgc ctacagcaat tgcggaacac tacggtgcta ccatgttcaa
    10441 tgtgttgaca gggtttaaat tcattgctga aaaaatccaa caattcgaag aagaccattc
    10501 acatactttt atgtttggat tcgaagaaag ttatggttat ttagttaaac cttttgttcg
    10561 cgataaagac gcgatccaag cattgctttt attagcagaa gtagcagctt actataaaaa
    10621 acaaggcaaa acgttgtatg acggcttgca aacgattttt gaaacctatg gctattttgc
    10681 agaaaaaacg atttctgtga caatgagcgg acaagaaggt tctgcgaaaa tctctgcttt
    10741 gatggaaaaa ttccgtaaag aagcaccgac agaatttgcc ggagcaaaag ttattcaaac
    10801 agaagacttt aaagaattga cgagaacaga ccgtgaagga aacgtagaaa aactaacaac
    10861 accgccatcc gacgtattga aatatacatt ggaagaaaat ggctggattg ctatccgccc
    10921 ttcaggaact gagccgaaaa tcaagtttta tattggtgta aaaggaagct ctacagaaga
    10981 cgcagccgaa aaagtagcct cttatgaagc gattatcaaa aagatgacag aagaataaaa
    11041 aacttgagac tgatggaaaa agtggtaaac tttagaagtc agtctttttt caagttggag
    11101 gtgaatggat tgccagacac gcaaaaatca aaagtagaga aagtcagtca attattcaaa
    11161 atgttgagtg atcccactcg tcttaaaatt ctgctatatc tgaaagacgg ggaacaaaat
    11221 gtgacagcaa tcacccaagc agtggaaatg gagcagtctg ctgtatcgca tcaactacgt
    11281 ttattaagag aaaatcatgt cgtcaagtct catcgcgagg gaaaagcgat tttgtatagt
    11341 cttgatgatt ctcatgtact ggatattttg aaccagacac tcaaacatgt ggaacatcaa
    11401 taacgatagg tgcaaataga taaacactgg agtgtgacaa cgaaatttct tcgttgtcac
    11461 actccagtgt tttttaagaa aaagacaaaa atgattaatc gcttttttct gttgtttcag
    11521 ttccataata actatgatct tctaggtcta aagcattcaa aataagcgaa gccgtttctt
    11581 caatagataa agatgccaca ttgatcacta cacatcctag tttttcataa agatcattcg
    11641 caaaatcgag ttcagctcta attttttcga tatcggaata agcagtatca gggtttaatc
    11701 cgtaagagcg catccgttct ttacggatat tatttaaaat atctgggtta ttcgttagtc
    11761 ccacgatttt tttaggatcg atctcccaaa gctgtttggg aatatgtgct tgtgggatca
    11821 aaggtaaatt ggcaactttt aaatttttgt tagctaaaaa caagctcaaa ggcgttttgg
    11881 aagttcggga tacgcctagc aaaacgacat ctgcttctaa aaaaccacga ggatctttcc
    11941 catcatcgta ttttacagca aattccattg ctttgatccg tttgaaatag ttctcattca
    12001 ataaatggag ggcaccaggt tgccgaattg gcttgatacc ggttaaacgt tcaatttctg
    12061 atatcggtgg cgtcatcaca tcaaaactaa ataattgatg ttcttcacaa aaagaacgtg
    12121 ttatctcgat cagttcttga ttgattaatg tatgcaggac aagtgctttt tcttctaatg
    12181 catcttgcaa tgcctgcttt aatgtttttt catctgtcac gaacgttctg cggaataggt
    12241 tgaactcaac ggtaggatac tgagccatag tagcttgcgc tagttttgtc gcagtttctc
    12301 cagcagagtc ggagatgaca aaaaaagtaa cgatttctgt agaatcggaa gacatggata
    12361 aaacctcact tgcatttttt ttatgtttat tataccatac tctagaatgc gtacttcgta
    12421 atcattttga tttggaggaa taagtcatga aaaaacagac attgatcgaa caatcattag
    12481 aaacattgaa aaataacggt tttaaatata ctaaaaaaag agaagcattg ttaacttatt
    12541 tagtgaagcg taaccgctac gtatctgcta aagaagtttt tgactatatg aatcaggaat
    12601 tcaaaggggt aagttatgat acgatttatc ggaatctcca tgattttgca gagatcgatt
    12661 tactggaaga aaccgattta aatggagaaa tgaagtttcg ttttcattgc tgtcaggggg
    12721 aaatcggcca tcaccatcat tttatctgta cagtgtgcgg aacaacgaaa gaaatccata
    12781 tgtgtccgat gaattatttc gaagaacagc taggtggatg cacgatcgaa gggcatagat
    12841 tcgagatttt cggaagatgt gaaaattgtc aataagggtt taaataaaat aacatttgat
    12901 aattttaaaa aaaagcaaga tttccttgac ggtcaaaaca gcattagata taatatttac
    12961 aggacagtct tgttttttga gatatatttc aataacaatt acggttactt tttaagctcg
    13021 gagggaggga attaacatgt caaaaacagt cgttcgcaaa aacgaatcac ttgacgatgc
    13081 tcttcgtcgc ttcaaacgtt ccgtgtcaaa agcgggtact ttacaagaat ctcgtaaacg
    13141 tgaattctac gaaaaaccaa gtgtaagacg caagaaaaaa tctgaagcag ctagaaaacg
    13201 caaaaaattc tagttctgtg atgaattgga gttggtagtg tgtcactact aagtagatta
    13261 aacgatgaca tgaaagcagc gatgaaagcg aaagataagg aatctttaca agtcatccga
    13321 atgatcaagt catctataca aaacgagcag attaaagaag gtcacgactt gacagaagaa
    13381 gaagaactaa cggtgttgtc tcgcgaaatg aaacagcgac gtgattcttt gcatgagttt
    13441 gaagaagcag gacgtgacga tttagctgaa aaagtaaaaa gtgaaattgt aatagtcgaa
    13501 aaatatatgc cggaacaact ttccgatgaa gaaattcgac aacttgttca agaagcaatc
    13561 actcaaactg gtgcttcatc aatgaaagaa tttggcaaag ttatgggtgc aattatgcct
    13621 aaagtcaaag gaaaagcaga tggaaaccaa gtgaatgcga ttgtgaaaga actattacaa
    13681 tcttaatttt atcatttaaa ctgcggacag tgattgtccg cagttttttg tgttcgtaaa
    13741 aaaagaa
//
LOCUS       DACDYI010000078        12679 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.078, whole genome shotgun sequence.
ACCESSION   DACDYI010000078 DACDYI010000000
VERSION     DACDYI010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.078"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..127
                     /locus_tag="IWM52_002366"
     CDS             <1..127
                     /locus_tag="IWM52_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP9974590.1"
                     /translation="RDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
     gene            120..1547
                     /locus_tag="IWM52_002367"
     CDS             120..1547
                     /locus_tag="IWM52_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAP9974591.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
     gene            1549..2619
                     /locus_tag="IWM52_002368"
     CDS             1549..2619
                     /locus_tag="IWM52_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974592.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYF
                     VYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYG
                     IDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTL
                     LISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLS
                     EEKILLPENWAELRKLVEYGII"
     gene            2606..4027
                     /locus_tag="IWM52_002369"
     CDS             2606..4027
                     /locus_tag="IWM52_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP9974593.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LLIISLVIYFLKIKNRIIDDYNS"
     gene            4040..5047
                     /locus_tag="IWM52_002370"
     CDS             4040..5047
                     /locus_tag="IWM52_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9974594.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            5059..6063
                     /gene="neuB"
                     /locus_tag="IWM52_002371"
     CDS             5059..6063
                     /gene="neuB"
                     /locus_tag="IWM52_002371"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAP9974595.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            6060..7208
                     /gene="neuC"
                     /locus_tag="IWM52_002372"
     CDS             6060..7208
                     /gene="neuC"
                     /locus_tag="IWM52_002372"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAP9974596.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            7181..7858
                     /locus_tag="IWM52_002373"
     CDS             7181..7858
                     /locus_tag="IWM52_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP9974597.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            7848..8990
                     /locus_tag="IWM52_002374"
     CDS             7848..8990
                     /locus_tag="IWM52_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAP9974598.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            9003..9980
                     /locus_tag="IWM52_002375"
     CDS             9003..9980
                     /locus_tag="IWM52_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9974599.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            9980..10888
                     /locus_tag="IWM52_002376"
     CDS             9980..10888
                     /locus_tag="IWM52_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP9974600.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            10889..11641
                     /locus_tag="IWM52_002377"
     CDS             10889..11641
                     /locus_tag="IWM52_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP9974601.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            11646..12593
                     /locus_tag="IWM52_002378"
     CDS             11646..12593
                     /locus_tag="IWM52_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP9974602.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT         4550 a         1572 c         2297 g         4260 t
ORIGIN      
        1 aagggatata gatcaaataa aagaaagtga aaaattattt gtaagaaaag ttaaatcgga
       61 ttactctgat aaactattaa atagaattga taaagaaatt ttgaaagttg gaaatataga
      121 tgaatagaat agtacctgtt ttagagaaga gtttattaag tcgagatgcg tttcgagtaa
      181 taaatttaat catttgtctg ctttatttta tgcctagtac ttttaatgta actaatatcc
      241 cgatgaaaat agcttttgct tggggtggaa ttatagtttt gtatgaggtt tttataaaga
      301 aaaatttttt taaaatgcgt ttttcttggc tactgattgg agcaatgatt agttttatct
      361 taactataat cattaacaga gaaaattttt taattccatc tatttataat tttggatatt
      421 tgctgattac tttgatagta atttttcctg cagacttttc acaaagtgaa gaggaaaaaa
      481 agaaaaaaat ggtgtatttt aataatgttt tcattatcat aattttttta gctgcgctaa
      541 tatctatcta tcaatttatt tttttgattt cttatcatgt cccaacagga acgccaggat
      601 tacttgcaag acaaggtttt attgaacatc ggttatttgg tgtctataca agtccaaatg
      661 ttggagctat gtttggatat acttctatta ttttaagttt aattagtgta ttaatttcaa
      721 aaataacaaa aacgattcgt gttttttata tgatgaattt tatcgtacaa atattttata
      781 ttacactttc ttcgtctaga ggtgcccagg tagtaatttg ttctttttta gtattatttc
      841 ttttgttgtt tgccaattca aaatttagaa agagcattag agaagtacta cctgtaaaaa
      901 tcaaatggat ggtaagcata ttattagttc tattggtata tttttctttt ggtactaatt
      961 atgctaagga ttttttagca acaattcctg tatcgattga tagaaaagta gaagttatag
     1021 agtcaaatga atcagataaa actccacttc cagaaaaaga agtaatatta cagcattcta
     1081 gtgataactc cgaaatttca gctggtcgtt ttacgatttg gaaagctgcg cttaaagtta
     1141 taagaccaac tttgttattt ggttatggag agaccgattt ctatgggaat agcgatgcaa
     1201 aatttttgaa aaaagtagat cttgatacta cagatgtgaa tgaactaaaa agagcacatg
     1261 gaaacatgca taatggattt cttcaagtac ttgtatcaag tggtataatt gcttttatat
     1321 gtatctatgc tttctatgtg ttaaatatct ttagtttact caaaacattt tttagaagta
     1381 aagtgaatta tgcgtttctt ggtattgttt taatttttat actatctatt tttatagatg
     1441 aaatggtgga ggctcatgta cttttcaata agcgcgatgt catttcaatt gttttctggt
     1501 attatttggg aagcatttct tacttgtatt taaaaaagga gaattaatat gaagacagct
     1561 ttcattgctt ggactccttt acatttgatt aatatattaa atgtttatca aaatttttac
     1621 cgagaatcaa aaggagattt atttatttat tctgaatttg aaaacgctag aagatatagt
     1681 gagcgtataa aagaattagg gctatttaat gatgttgttt ttgtgaatca ccggaaaatc
     1741 ggttcttcct ttaaacgtaa aattaacttg ttaactaacc ataatcaatt tttctcggat
     1801 gatttatccg tttatgatga aatttttata cagggggaaa attatttcac aaaaatactt
     1861 ttctccaatg tttacaaaaa aaatccagat gtaaaactaa attatataga agatggtttg
     1921 ggtacttatt tgaagaagag tatattttca acaagtaaaa acgttcaaag agtttttaaa
     1981 atattaaata aaaatagcat cttcaaaaaa aaaattgacg attattttgt ctattgtccg
     2041 gaattgttac tttatgatgt aaaaaatata aaaaaaattc ccaaaataga tgaaaacagt
     2101 aaattgatga aaaacataca atcaatcttt gggatagaag aagtttttgt cggagataaa
     2161 tgtatctttt ttgatcaacc tttattatct gatggttatg gcatagatga aacagatatt
     2221 ttcgctaaag taaatagtat attttctgga aaaaaagaaa ctttaattaa gcttcatcct
     2281 agagcaagga caaatcgtta tggtgaaaaa aacatattga attccagttt accgtttgaa
     2341 gtcttgctac ttgaaaatga cttctcttca acacttttga ttagtcctat atctacaatt
     2401 tctttttcac catctatgat gtttgaaaag acgatcaact ctattatgtt ggctaaatta
     2461 attttaaatg aatatacagc attatctgaa gataaaaaag aaacattgac aaatattgtt
     2521 cttttctgtg aacattataa ccgtctatca gaagaaaaaa ttttactccc agaaaactgg
     2581 gcagaattaa gaaagttggt agagtatggg attatttaac acactttcca agtcaaaatt
     2641 agttaagtcc agtttctggt atactatagg gaattttttt attaagggaa tttcattcat
     2701 aactattcct atttttgtaa atattactac tcaagcagaa tatggtttga ttaataattt
     2761 cacttcattt gtatcaattt tttcgattct tgttgggcta agcctaaatt cggcaattaa
     2821 taatgcaagt tttgactata aagatgacat aaatgaattt atgtcatcgg tactttctct
     2881 agctacactt tcgttaattg tgttcttatt aatagggaac atatattttt tgttttcaaa
     2941 tgcttttttt gatatgaacc aacttgtatt taatttgatg ttattacaaa gttttgctag
     3001 ttttgtaatt agttatatcc agtcttattt tacgattaac tttaaccatt ttaaatattt
     3061 gattttatct tttatatcta cttttttgaa tattgcaatc tctgtagctt tgatgcagac
     3121 attatatttt gatgataaat atgttggcag agtagtcggc ggaacactgt catttgtgtt
     3181 gataggttta gtaatttatt taagaataat gataaagggt gctcattttt ttgataaaaa
     3241 acagtggaga tacgctttaa aaatctcttt gccgctaatc ccacattcct tagcaaatat
     3301 agttttagca caattcgata gaattatgat taattcttat ttaggtcctg ctgcgggggg
     3361 aatatattcc tacattagta acataggtat tattctaagc gtaatatgga tgtctactaa
     3421 taacgcctgg gttccatggt tctatggtga gatgagtaaa aataatgaaa aaaaagtgaa
     3481 gaaagttagt aactattatt tagtagtctt tactttgatt acgatgattg taatggtagt
     3541 ttcgattgat ttagcaaaat ttatggctcc gaaagactat tattctgggt tatctttagt
     3601 ggttccgata accttaggct atttctttca gtttttatat agtttaccag ttaatgctga
     3661 attttatgag aaaaaaacat cgtttattgc gattggtaca gtagcaagtg ctatagttaa
     3721 tatactatta aacatgattt tcatacctag gttaggaata attgctgctg gatacacaac
     3781 cgtatttacg tattttttac tttttatttt tcattataat atcgctaaaa aaattacgaa
     3841 taaacaatta tttgacacaa aaaaaatatg tatcataata atttttgtat caattttttc
     3901 tttgatactt catattttaa tcaatcaaat gttgattcgt tacgttttga catcattatt
     3961 gataattagt ttagttatat attttttaaa gattaagaat cgaattatag atgactataa
     4021 ttcataggag gagatataag tgtttaaagg aaaaacaata ttagtaactg gcggaactgg
     4081 ttccattgga agtgaaattg ttcgtcagct tttaaaatat gaaccaaaag ctatcagaat
     4141 ttatagtaga ggtgaagaaa aacaatttta tatgcaacaa gagctctcaa atcacactaa
     4201 tttgagattt ttaattggtg atatcagaga ctctcaaagg attgattatg catgtcgtga
     4261 tgtggatatt atttttcatg ctgccgcaat gaaacacgtt ccagcaagtg aatataatcc
     4321 tatggaagct gtaaaaacaa atgtgatggg aactcaaaat gtaatagatg cagcgatcaa
     4381 aaataatgta aaacattttg taggtatcag tacagataaa gtagtgagtc ctactaacac
     4441 catgggtgcg acaaagttat tgagtgaaaa attaataata gctgccaatc agtataaagg
     4501 aaaaacagaa acaatatttt cttgtgtaag gtttggaaat gtcatggggt caagtggatc
     4561 tgttattcct ctttttagta aacaaataaa aaacaataaa gatattactt tgacagatgt
     4621 tgatatgaca cgatttatga tgtcaatacc ggatgctgca agattagttc tgtctgctgc
     4681 tatgaattca aaatttggag aaacatatgt cttaaaaatg ccggcaatca atattttaac
     4741 tttagctcaa tcattaataa aaatatataa tgatacacat gatgaaaaat atagaggtga
     4801 aataaaagtt gtaggtatca ggccaggaga aaaattatat gaagagctaa tgactacaga
     4861 cgaaattgaa cgctcatatg aaaatgataa aatgtatatc attccaccac actatcatgc
     4921 tactgacgaa ctgtacaaag attatgaaaa aattaaaatc actgcatact cttcagatgc
     4981 aacagaatta atgagtggac aggaatttat tagaatcgca aaagagaata atgtcgtgac
     5041 ttggtaagga gttttatgat gaaatcagaa gtatatgtaa ttgcagaaat tggtgtaaac
     5101 cataatggaa gtctagattt agctttagaa agtattgata aagctaaaga atgtggagct
     5161 aacgcggtta aatttcaaac atttaaaacg aataagctag tttccaaaaa ggctccaatg
     5221 gcaaaatatc agaaggataa tcttaaagag gacagttctc aatttgaaat gctaaaaaaa
     5281 ttagaattat ctcaatctga ttttattaga ataaaagatt attgtataca aaataaaatt
     5341 gattttttgt ctactccttt tgatgaagaa agtgcagact ttctaaaaca aattggtgta
     5401 caaggattta aaattggctc aggagattta acaaatttac cttttcttag aaaaatagat
     5461 atgtatgggt tgccagtttt attatctaca ggtatggctg atattcatga ggtcagtgag
     5521 gctgtagatt gtttcataga tagtccagtt actattttac attgtacttc aaattatccg
     5581 gctagttctg aagatattaa tttattggcg attaacacat taaaagaaat gttcgggata
     5641 cccgttgggt actccgatca ttcattggga tatgatgttg gtatttgtgc tgttgcgatg
     5701 ggtgctaagg taattgagaa acattttacc ttggataagg aactacctgg tccagatcat
     5761 aaagcttctt tagatcctca ggaatttgca gattttgtaa agcatattag aaacactgaa
     5821 atatttttag gtgatggtgt taagcgagca atgccttctg aacttagtac aaaagaagta
     5881 tcaagaaaaa gcctagtggt cactgagaat ttaaatgtgg gagatattct tacagagaaa
     5941 tctcttacaa taaaacgtcc tggaaatgga atagctccta agtttttata tcaatatatt
     6001 ggcaaaaaag ttaaaagacc aataagtgca gatagtgttc tgcttgagga agatgttgaa
     6061 tgaaaaaaat acttatagta agtggaacac gagctgatta tggaatatat aaacctgttg
     6121 ctgagaattt actacaagat aattgggatg tatcatttgt tgtttctgga atgcatttat
     6181 cccataaata tggatttact aaagaattga tcttagctga cggatttaga attagtggag
     6241 aagttcaaag tttgttttta gagaacaata agggaaacat ggctcggtct gtggcattag
     6301 agataaatgg tttcacaaat atatttgaac aaactagccc tgattttgta ttattactag
     6361 gggatagagg agaaatgttg gcagctgcga tagcttgttt gtatcttgga atcaaaacag
     6421 cacatattca cggaggcgaa gtgtctggta ctgtagatga atcaataaga catgccataa
     6481 cgaaaatagc atctgttcat tttgctgcta cggaaaaaag caaagagcgt ttaataaaaa
     6541 tgggggaaga ttcttggaga gtttttcccg tcggagctcc tagaatagat actattttaa
     6601 ataatagatt acctagtttt agagatataa aagaaaaata tcatctaaaa tttgaaaaga
     6661 aaaaatacgt tttgcaagta tttcatccag ttgtaaccga actagaaaat atagaaaaac
     6721 aaattttagc tttgatcaat tcaatggaac aacaatctca accaattatt tgcattttac
     6781 ctaatagtga tgctggtagc gaaattatca gaaaaatgta ccagaaatat ttaaaaacgg
     6841 atattattta tattacgaat ttagagccgg aggaatacct gactatatta aaagagtgca
     6901 aatttatgat cggaaattct tcttctggga taattgaagc tgcatcattc aaaataccag
     6961 taataaattt aggaaccaga caaaatggac gagaacaatc taaaaatatt ataaatgcaa
     7021 atctaaatga aaatgaaatt gaaactgctt tgaataaaat acaagatcct cagtttataa
     7081 aaagcttgaa aaatgttaag aacatctacg gtgacggcaa cagttctaga agaataacaa
     7141 aaacattgaa tcaaatattg aatgatgggg aagatattaa gtggattcaa aaacaaatag
     7201 cttattaaaa tatactagtg atccatacat tattattggg gctggcggac acgcaaaagt
     7261 cattattgaa accttaagat tagaaaataa gttaatatat ggattttttg atgataagca
     7321 aaataaaatg caagatctga aatatcttgg gaaaataaac gaattgaata gctgggcgga
     7381 tagcaaaaaa aaaatctata aatttattgt agctattggt gactgtgtag taagaaaaaa
     7441 gattataaaa aaattgaata tgccaagtga gcaatatgga atagctgttc atccaagtgc
     7501 tatagtggca aaagaaattt caattggtta tggaactgtt atttttgcga atgctgtaat
     7561 taacccagat gctgtaattg gagagcatgc tataataaac acaggtagca ttgttgagca
     7621 tgattgtaga attaataact atgttcattt atctccaggt gtatgcttag cgggtggagt
     7681 acatgtaggt gtaggaactc aaataggaat tggttctcaa tgtatccaga tgaagaaaat
     7741 tggatcttgg tgtatgatag gcgcagggag cactattgtt aaggatattc cctcacattc
     7801 cttagcttat ggcaatccag ctaaaattaa gaaagaagga attaattttg aattctagaa
     7861 ttttattatc aagtcctcat atgggaggaa cagaagaaaa atatgttgct aaggctttta
     7921 aagataattg gatagcgcca ttaggtgaaa atgtggataa atttgaagaa agtattacaa
     7981 aatatactgg ttctaagggc ggagcttctg cgttaagttc aggtacagct gccatacatc
     8041 ttgccttaag actgcttaat gttggaaaaa acgatattgt ctttgctcct tcatttactt
     8101 ttatagctac agtaaatcct attttatatc aaggggctgt accagtgttc attgactcag
     8161 aacctaatac atggaatatg agccctgttg cactagagag cgcattagaa gaagctgaaa
     8221 gaaacaacca attacctaag gcaatcatcg tagtgaattt atatggtcaa atggctgatt
     8281 atgaacgaat tttagctatt gcaaaccaat ttaatgttcc agtgatagaa gatgcagcgg
     8341 aatcgttagg ttctacttat aagggaattt cgtcaggctt atttgggaca attgggatct
     8401 attcatttaa cggtaataag atcatcacaa cctcaggtgg aggagctcta gtgtctagtg
     8461 ataaaaaata tgttgagcat tcaagatttt tagcaacaca agcaagagat caagcaccgt
     8521 attatcaaca tagcgaaata ggttataatt accgtctaag caatatttct gccggtatcg
     8581 gacgtggaca aatggaagta ttgaatgata gagtagctaa acgaagagaa attttcaatc
     8641 gatataagga agctttttct ggaattgata gaattgaaat gatgccagaa gtggatggca
     8701 tggtgccaaa tcgttggttg tctactttaa cgatcaatga ttataattct attgatcaag
     8761 tagttaacgt aataaataaa ctaaatgaaa aaaacattga aacaagagct ttgtggaaac
     8821 cactacattt gcaaccagta tttcaagggg taaaaatgta ttaccatgat aataatctta
     8881 gtgtgtcaga aaagttattt aatagcggct tatgcttacc ttctggaagt aatatgacag
     8941 atagtgaaca agaatttgtt attgatgagt ttaaaaaaat attcaagtag gtgtgatatt
     9001 ttatgaacat aggaattatt ggtttaggtt ccatggggaa aagaagactt agactcattc
     9061 aagaacactt taatgatatt ttactatgtg gtgttgatac taatgaggcg agacgaaagg
     9121 aagttgaaaa ggaatttgga ataccaactt ataatagtct aacagaggca aaaaatttgt
     9181 ttgaaataaa ttcagttttt gtttgtacat cccctataac ccatgaattt attataaaag
     9241 aggctttaaa tttagattgt aatatcttta cggaaatcaa tttattaaat gaatattatg
     9301 atgaaataat cgaagtagct aaaaagaaag atagattact ttatctatct tctacatttt
     9361 tgaagagaaa agaaattcaa tatattaagt ctaaagtgac gcataataaa aaaatttctt
     9421 atcaatacca tgtaggacag tatttacccg actggcatcc ttgggaagat tatcgcaatt
     9481 tttttgtttc aaacaaaaaa actaatgggt gtagagaaat attcgctatt gaattacctt
     9541 ggattataga tacatttggg aaagttaaat cgtttactgt aatgacccag aaattatcca
     9601 atcttgatat tgattattcg gacagtttca ctcttattat tgagcatgaa actggtatag
     9661 ttggaacttt aagtgtagat gttgtttcaa gagtagctaa aagaaattta actataattg
     9721 gtgaaaagat tttaatagaa tgggaaggta acccggattc tctattttca tggaatgaag
     9781 aaacttctag tatgacagat attcaattgt acgatgaggt tatgcatgat aataattatt
     9841 cacatactat tattgaagat gcatatttag aggagattaa agaatttttc acgcttttgg
     9901 aaaagaaaat ggaaccccaa tatacttttg aaaaagataa gtatattatt aacttaatta
     9961 acgacattga ggtaaattaa tgatgaaagt aatttttttt gggttagggt caattggaaa
    10021 gagacatcta aaaaatctaa taagctatgg aaaaaagaat tctattaatt ttgatataac
    10081 agcttatagt agcagtggaa aaaaagagtt tgaagaaata acgtatattc aaaatagaga
    10141 cgatttggat acacattatg atatagcttt tattacaaat ccgactttta agcatctgga
    10201 aacactaaat gtaattgttg aaaaagttga ttacatattt ttagaaaaac ctgctttttc
    10261 tgagagtatt gatcttactc aatataatta tgattacaaa aatatttatg ttgctgctcc
    10321 actgaggtat aaaaatatca tggaagttgt tcgtaatagt attcaaagta aaaaagtata
    10381 ttctgtaaga gctatttgtt ctacttatct tcctgattgg agaaaaggta ctgattatag
    10441 aaataattat tctgccttta aatctatggg gggaggagta gaattagact gtatacatga
    10501 attggattat attataaacc tatttggttt ccctaaatca gtaaaaaaac agtttggtaa
    10561 atattcgaat cttgaaatta acagtaatga tttagcaaca tatatattga gatacgatga
    10621 taaaattata gaaattcatt tagattattt tggaagagtt cctacaagaa aatttcaatt
    10681 gtttacttta gatgaatgta tcaatgttga cttattaaat aataaaataa gttttgaaga
    10741 aaacattgtt aaagaaatgc ctgaatctgc taatgatatg tatgagaagg agttgaaata
    10801 tttctttgaa aacgtagtaa caggcaaaga aaactggaat gatttatatc atgcaaacga
    10861 agtattgaaa atagctgagg agaaataaat ggaaatttta tttactattt gtggtagagc
    10921 tgggtctaag gggtttaaaa ataaaaattt gaaatatttg gcaggaaagc ccctagtcca
    10981 tttcgcttta gcaatgatag atatctatca acaaaaacat cctgaacata attgcacgac
    11041 ttgtttgaat acggacagta atgagttagt agaattgacg actaagttaa atccgtctgt
    11101 gaaaattgta gaacgtaaac ctgaattagc tacagatact gtagggaaaa ttgaagtgat
    11161 acgtgattca ttcttacaac tagaaagaga agataatata tttgatttaa ttatagattt
    11221 agatttgact tcacctttac gtgaagtttc taatcttgaa gaattaatag atgaacatct
    11281 aaaaaattta gataaagacg tctacttttc agttgtttct gcaagacgta atccctattt
    11341 taatatggta aaaagaattg aaaatgaagt tactatagtt aataaatcaa gatacacatc
    11401 taggcaacag acacctgaag tttttgaaat gaatgcctca atgtattctt ttaaacctga
    11461 atttctaaaa aaaagtaatt atatttttga tgggcaatgt gggataataa aaatgagaga
    11521 ttatctaatt ttagatattg atagtgaaga agattttaaa tggttagaat ttttgtttcc
    11581 taaattttta gaagattctt cggagcttca agatatctat aattatttat atcggaggta
    11641 aaaggatgag ctgttatctt atcaccggtg gggctggctt cattggttct accttagcca
    11701 atcatttagg agaagaacat gtggtaatcg tcgtcgatga cttatcaatg ggaaaagtag
    11761 aaaatcttga tatgacgaaa aatatcactt ttattgaggg agatgtcgct gagaaatcct
    11821 tgatggaaga gatcatgaaa gctaataaat ttgattatat ctttcattta gcagcagttg
    11881 ctagtgtggc agattcagtt gcccgaccag tagaaacaca tcgtgtgaat tttgaaagtg
    11941 tattaatgat tttagaattg atccgcaagt atcaacctga tttaaaacgg atcgtgttta
    12001 gttcttcagc ggctgtttat ggggatgaac cgactttacc aaaaaaagaa gagtccgtga
    12061 ttcgcccatt gacgccttat gcggttgata aattcgctgc agaacaatat gtgctagatt
    12121 attgtcattt atatgatgta cccggtagcg cagttcgttt tttcaatgtc tatggtccaa
    12181 atcaaaatcc gaattctcca tacagtggtg tgatttctat tctcgtagat cgctataaga
    12241 aacaaatcgt aggagaagca acttcattta cattatttgg tgatggaagc cagtctagag
    12301 actttgttta tatagatgat gtaatccaag cattactttt agttgcgaat gaggaagctg
    12361 cactggggaa acaattcaat gttggaacgg gtaaagcaac cacacttttg gaattgattc
    12421 atgcaatcaa tgaaattctc ggtgtagaat tgacacttga atacaaagaa gagcgttcgg
    12481 gtgatatcca tgactctcta gcagatatta ctaagatcca gtcaattggc tatcaaccga
    12541 aatatggtat tttaagtgga atgactaatt atttgaaaac tgaaatcaat taaaacatac
    12601 taagataaga ttagagggta aattcaggct ctttgtcaat aaggactgat gagttttgta
    12661 aaatcaaatc tgggataga
//
LOCUS       DACDYI010000079        12670 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.079, whole genome shotgun sequence.
ACCESSION   DACDYI010000079 DACDYI010000000
VERSION     DACDYI010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.079"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            6..305
                     /locus_tag="IWM52_002379"
     CDS             6..305
                     /locus_tag="IWM52_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9974603.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IWM52_002380"
     CDS             378..1739
                     /locus_tag="IWM52_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP9974604.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IWM52_002381"
     CDS             1761..2021
                     /locus_tag="IWM52_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974605.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IWM52_002382"
     CDS             2035..2787
                     /locus_tag="IWM52_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP9974606.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IWM52_002383"
     CDS             complement(2881..3486)
                     /locus_tag="IWM52_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9974607.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IWM52_002384"
     CDS             complement(3539..4513)
                     /locus_tag="IWM52_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAP9974608.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IWM52_002385"
     CDS             complement(4891..5670)
                     /locus_tag="IWM52_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9974609.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IWM52_002386"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IWM52_002386"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP9974610.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IWM52_002387"
     CDS             7161..8558
                     /locus_tag="IWM52_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9974611.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IWM52_002388"
     CDS             8639..9079
                     /locus_tag="IWM52_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9974612.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IWM52_002389"
     CDS             9227..9553
                     /locus_tag="IWM52_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9974613.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IWM52_002390"
     CDS             9554..10303
                     /locus_tag="IWM52_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974614.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IWM52_002391"
     CDS             complement(10383..10838)
                     /locus_tag="IWM52_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP9974615.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="IWM52_002392"
     CDS             complement(11265..11540)
                     /locus_tag="IWM52_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974616.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="IWM52_002393"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="IWM52_002393"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAP9974617.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         4055 a         2175 c         2587 g         3853 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag
//
LOCUS       DACDYI010000080        12659 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.080, whole genome shotgun sequence.
ACCESSION   DACDYI010000080 DACDYI010000000
VERSION     DACDYI010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12659
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.080"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..529)
                     /locus_tag="IWM52_002394"
     CDS             complement(<1..529)
                     /locus_tag="IWM52_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9974618.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIV"
     gene            complement(526..1179)
                     /locus_tag="IWM52_002395"
     CDS             complement(526..1179)
                     /locus_tag="IWM52_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9974619.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1467..1987
                     /locus_tag="IWM52_002396"
                     /pseudo
     CDS             1467..1987
                     /locus_tag="IWM52_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1889..2605)
                     /locus_tag="IWM52_002397"
     CDS             complement(1889..2605)
                     /locus_tag="IWM52_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAP9974620.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(2655..2864)
                     /locus_tag="IWM52_002398"
     CDS             complement(2655..2864)
                     /locus_tag="IWM52_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974621.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            3141..4414
                     /locus_tag="IWM52_002399"
                     /pseudo
     CDS             3141..4414
                     /locus_tag="IWM52_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4521..5552)
                     /locus_tag="IWM52_002400"
     CDS             complement(4521..5552)
                     /locus_tag="IWM52_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9974622.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(5559..6389)
                     /locus_tag="IWM52_002401"
     CDS             complement(5559..6389)
                     /locus_tag="IWM52_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9974623.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(6386..7192)
                     /locus_tag="IWM52_002402"
     CDS             complement(6386..7192)
                     /locus_tag="IWM52_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9974624.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(7198..8022)
                     /locus_tag="IWM52_002403"
     CDS             complement(7198..8022)
                     /locus_tag="IWM52_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAP9974625.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(8012..9112)
                     /locus_tag="IWM52_002404"
     CDS             complement(8012..9112)
                     /locus_tag="IWM52_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9974626.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            9290..10075
                     /locus_tag="IWM52_002405"
     CDS             9290..10075
                     /locus_tag="IWM52_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAP9974627.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            10108..10491
                     /locus_tag="IWM52_002406"
     CDS             10108..10491
                     /locus_tag="IWM52_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP9974628.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            10567..10698
                     /locus_tag="IWM52_002407"
     CDS             10567..10698
                     /locus_tag="IWM52_002407"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9974629.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            10667..10903
                     /locus_tag="IWM52_002408"
     CDS             10667..10903
                     /locus_tag="IWM52_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9974630.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(10981..11952)
                     /locus_tag="IWM52_002409"
     CDS             complement(10981..11952)
                     /locus_tag="IWM52_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAP9974631.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     GSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         3820 a         2814 c         2278 g         3747 t
ORIGIN      
        1 taacaatttt tatttcttgt atattatttt ttattgattt tgcataaatt aaagaaataa
       61 tagcagaaaa taaaattgat attaataaag aatatggtaa aaatttaagg cttaaatctt
      121 tcgcatcttt ttgcatatca gctgtagaaa caaattgtag atttgttttt ttaccatcat
      181 taagctttat ttctctttct tcaattatca aagaattact atcactttct aaattaacat
      241 ttatattatc tttgatttgt atttcattct tattattttt ttcttttatg aatactttta
      301 tttcactact cttggaataa aactccaaag tttgttctat atattttatt tcttttccat
      361 tcatattact agaaatttca tttgccatat tatgaatctt ttcttttctc gtctccaaat
      421 atgttttagg aaagataaaa aaaattaatg aatgaactaa tataattatt attccaagaa
      481 cagaaaatat ttgtatgaac atttttggaa atatctttaa ttttttcatt ttctctccaa
      541 tttatatcct acatttctta tagtagttat acagtctaat tgtaattttt ttctaagttc
      601 ttttatatat acatctatta ctcgatcata aggagtttct tcgctatctt tccatacata
      661 atcaataatt tgcattcttg ttaacacttg tccattatta tccaataaac attttaatac
      721 ttctagttct tttgcattta catctatttc ttcaccattt attttagctg tatagctatt
      781 aaaattaacc gatagatcct tatattcaaa cttctctaaa tgtccataat tcttcttaat
      841 taaagaatct attctagctt ttaagaccgg caatgaaaat ggcttttcta catatccgtc
      901 tactaaatta gtaaatgcat caattttata ttcttcatca ctaaatgcag tcaagattag
      961 aattggtaaa ttgcttttct ttctaatttc tttcaacact tctaaaccat ttataaaagg
     1021 tatctgaatg tctaagataa ccaaatttat atcgctatta aattttgaca aggcttctct
     1081 tccatcttta gcttgaataa cagtatatcc aaattctgaa agatattcac ttattccctc
     1141 tcttatcata ttatcatctt caacagttaa tattttcata tatacctcca cttaagccta
     1201 tacactagat attatacagc atattatggc tctataatat ctagtgttgg tgacgccttc
     1261 tgttgccaat actttttctt ttttttaata aatgcaaaca gacaccttga aatcctttag
     1321 aaaaaaggct tctataatat tttgtgttgt tggaaaatcg aaggcgattt tctactaaca
     1381 ctttttcttc gtttcaactc aaaaagagac tcacttttgg taaaatttag tcgaccaaaa
     1441 catcacccta ccaaaggagt cgctctatgg ataatcatac tagaaaatta ctcggattaa
     1501 cagataaaca tttatttttt gatgaagaat ggctgattga aaaagaatat aaaggcgttc
     1561 aagcgcagtt tattaaaggg agattggatg actcaccttc ccattgtgaa cactgtggat
     1621 cgattcgtat tatccgcaac ggttcctata cgacacgtac tcagatgttg aaagtcaaag
     1681 aaaagttgac tattttagaa ttaaaacgta cccgttttct atgctatgat tgtgggcaga
     1741 ccttttctgc gaaaacggac ttagttgatg aacatcatca actaacaaag gaactgaagc
     1801 aggcgattct catagaactg tacgaaaatc aatcacgtaa tctgaaaatc agaagaagta
     1861 ccgtcggctc aagcgatatt ggaaactctt actcaaagat tccacgacat tggagccgct
     1921 caaacggcat tatcatcgac tgtttaaacg accaattagt caaacagaaa tcgtcgatga
     1981 accgttgtgc acaatggtgt cctgaacgta cagcgacacc ttccatatcc agagcagtcg
     2041 ccacatcacg gggatggatg ccatccaaat taaaagcaac cacgccagta tgatgttcag
     2101 gatcttgcgg tccgtaaatg gtgataccgc cgatcgcttg taatttaggt aaaacgtagg
     2161 caaccagttc ttgctcataa cgatgaatat tctccatacc aatctcattc aagtaatcga
     2221 ttgctcctgc aatgttcgga gtaccagctt caaatttcca tggcagctct ttccatgaac
     2281 tatcgtaaag attgacaaaa tctatcattt caccgccgaa ttcgactggc tccatttgtt
     2341 ctaacaattg acgtttgcca tataatacac cgattcctgt cggcccgcac attttatgcc
     2401 cgctaaaggc gaaaaaatct gcatccaacg cttgtacatc aactgtcata tgcggtgctg
     2461 cttgggcacc gactggacta tcctcaacta caacagcctg ttcaaccctc agccgtttca
     2521 agcacaacaa ataaccttct ggatctggtt tgccgacctg aagatcatcg atcgtcacaa
     2581 aggaatcaat tttttcctta atcatcggat gtgcttcaaa aaaatagtct agctcccgca
     2641 gttcagaatt gagactactc cgattttcca gtgctgctga tccgctgctc aaaatgttca
     2701 gcatccactg gtctcccaac cgcttgactt atcccttcat aaaaggacag ttctgctgcc
     2761 gtcccaacac cttgttgata gtctgcaaaa ggaattttat agccgaactg ctccagtaaa
     2821 taggcttcag taaatagtga tgaatactct cgtaaaacat ccattcactg tcaatcatcg
     2881 tcccatcaaa atcaaaaata agtgctttgg tcatcttctt tcccccttac ccaaaacaag
     2941 caaaagctgt gtcctgctct ctctacagta tcagcataaa aactgccgtc tgaaaagtca
     3001 aaggtgaaag aaatgtggcg ctataatttc taggactgat agtaaatcat tctatcagtc
     3061 cttttgagca taaaaaaaca aaacatacag aagctccgtt agaattgaat caccacaaca
     3121 caatcaaagg agcgttctgt atgcctgatc ttcatcttat caaactatta ctaggaatca
     3181 aagatggaaa cattcaaatc actcatgtcg agaagaaaaa aatacattca atcaacagta
     3241 tcgttgtttc tgccacgctc attaaaacga ttcagccctg tcctcagtgt aaacaggtga
     3301 atcaacatgg gctgatcgtt aagaacggaa agaaaaaatc gctggtcctt ctcaataagt
     3361 gtgcgaatca acggacttat cttgctttgg caaagcaacg gtacctctgt cgtggctgtc
     3421 acacctattt tacggcgaat acctacattg ttgaaaaaaa ctgttttatc gccaaacaag
     3481 tacattataa aatattggag gaattgactg aaaaacaagc aatgacgacg atttctaaac
     3541 attgcggcgt ttcttggtca accgtttcgc gaacactcgc ttctctgctc cccatgacaa
     3601 acgtgaaaat aaattggttg cctcgctgtt cattggtaga tgaatttcgt tcattgaaaa
     3661 atcaagtcgg tccctattct ttttcttgta tggatggcga tactgggaaa ttactggata
     3721 ttcttccttc acgtaagaag aaagatctca tttcttattt tatgcaattt gaacgacgtg
     3781 cacgtttaaa cgtaaaaata ctcgtaacag atatgaacgc ctcttatgct ttgctgataa
     3841 aagagtgctt tcctcatgcc aaactcgttg tggatcgctt tcacatcgtc aagcacctca
     3901 ttcgcagttt taaggatatt tgactatgag tcatgaaaag cttcgaccga aacgatccaa
     3961 tacaagccaa acactatcga caagtgaagg ctttatctcg acttctgatc aaacgacagg
     4021 acacgcttgt ttatgataaa tggacaaaat ggcgtaattt tggttgggcc tatttaaccg
     4081 aaagtgaagt cgtagaacgt ctactttcta cttctgatga actacgaata gcttatgctt
     4141 actatcaaga aattcttcaa gctttttatg acaaagaagc ggacatcttc tttcagcttg
     4201 taaaaacaat gcccaagtcg gttccaagag aactgcatca tataaaaaaa gcgtttataa
     4261 actatgaatc cggcattcgt ttagccttag agttgcctta ctcaaatggc aaaatagaaa
     4321 atctgcatac ccacatcaaa gcattgaaac gaatagctta cgggttagga tgagaaccag
     4381 aattttctta atcaatcatc tgatcactta ttaaacataa aaaaacatct aaagcagaaa
     4441 atcaattcta ccttagatgt tcttatatca ctctatcagt cctatttgac aaagagccag
     4501 aaatgtttgc cgactataca ctagctttgt tgtgcaaaat gctgagtatc aatttccaca
     4561 gggattgcta gaatatcttt cagctgaatg tataaggcat cgctaatttt ctcttcccga
     4621 tcgttcaacc gatccactaa aatttctttg ccctccagcg tttgaaaaac ataccgttga
     4681 atgttgccta aaataaatga ttccttccat agaaccggaa tcttaacccc ctgaggaacc
     4741 ggttcaaatt gaatcagctc cggtcttaaa taataaatgc agcgattgcc cttagcggaa
     4801 aactcgtggg tatactgatt cagctcatac cattcgaaac gattatagtt gccgataaat
     4861 tccgcaacaa atcggctctg aggattctga tacaattcct tcgaggtgga aacctgagcc
     4921 acccgcccag cttccatcac aaaaatccga tcactgattc gcatcgcctc ttcttggtca
     4981 tgagtcacaa aaatcatcgt catccctagc gattgctgga tagcgcgcaa atcccgctgc
     5041 agctgttttc taattcgggc atccaaagca cttaatggct catccagcag caggactttc
     5101 ggttcagtga ccaatgagcg agccagtgcc acccgttgtc gttgaccgcc agaaagaaac
     5161 tggacatttt tatctgcctg ctccgtcata tccaccaatg atagcatctc aaaaaccttt
     5221 tcctgaatca cctcaggact cagcttcttt accttcaaac caaaggctac attttcaaaa
     5281 acagtcatag ttggaaacaa tgcatactgt tgaaaaacaa agccgatatt gcgatccttc
     5341 gtaggcttat cggcaatatt ctcattgtcc agcacaatct caccgctctt tggttgttcc
     5401 aatcccgtga tacagcgcag caacgtagac tttccgcacc cgctgggacc tagtaaagta
     5461 accagctcac ccttttcaat ctgaatttca tcaatagcta aaatctgttt ttcatcaaaa
     5521 aacagattga gatcatgtat ttctaaaaac attttccgtc acaactcctt taagggcttc
     5581 tttttcgact gcttctcttc cctcgtctgc ttaagggaca gctgttgctt ttgtcgataa
     5641 gtcagcataa aggtagttaa agcaattccc accataaaca gcaaatacaa aaccatcaca
     5701 gcgctagcca aatggccgtt ttcgttcatc cgccgcagca tatagatgcg taccgtctga
     5761 aatcgaccgc caatcaacag gttcgtcagc atgtactccc caaaaaccga agaaaaaatc
     5821 atcagagaaa ctagggtcac accgatacgg atgtttggaa acagcacctg gataataatc
     5881 gtagacatcg gggcgcccaa ggtttctgct gcatccacta attccttcag attaattaag
     5941 cgcatctggt tgttaagact aagatagaca atcggcaacg ccgtaataaa caatgcacct
     6001 accaacacta caaacattgg gataccagtt tcagcatacg ttcgaagcaa agccgttacc
     6061 aaaatcactc ctggtaaagc atacggcagc aggagcagct tttccagcca acgattcaaa
     6121 cgagggaaat acaaatgaat ccaaataatg gtgggaatca tcaacaacag aatcgtcacc
     6181 aagaccacac ctgctaataa aagtgagcga cccaccgcag caataaattc cctgtccgtc
     6241 agcagctgtt ggaaccattg caaggtaaaa tcactaggta aaagcgattt tgaccattta
     6301 gtggagctgg catacaaaat cgttccaata atcggcagca agtaatacag gacaaagcag
     6361 aacaaaatcg gttttattaa ctttttcacg tctgcaccac cttgcctttc ttttgaataa
     6421 tcaacattgc cgttaccagc aggacaccta aagcgactgc caacgcactg cctaaattag
     6481 gcttggcgaa cacatctccc gacactaaag ccgcaattcg aatcgttaaa agatttacat
     6541 tactgctggt cagggcatac gctgtttcat aggtccccat gccattagca aataagatga
     6601 caaaggtgct ggcaatcgat ggtcgcaaaa aaggcaaagc caccttgaac caagcatacg
     6661 ctgatgaagc gcccagcaat tgtgcggact ccagccattc ctttttcact tccttcatgc
     6721 tgggatacaa aaacaagacc cctaacggaa tttggaaata ggtatacgtg atggtcaatc
     6781 cccaccaaga atacaaattg aagtccgcta gaaatggcaa aatcattttc agcacaccta
     6841 cattccccaa caaaataata aaagagaatg ccagcggaat cccagcaaaa ttagctgcca
     6901 aattggaaac aaaggtgatt ttctcctgca atttagtcga tagattcatt aagcataaac
     6961 ataaaataaa cgtcccgatt aacccaatca cactggagct gacagcaatc agtacgctgt
     7021 taacaaaggc ctgtgtgtaa tatttgttgc tgaaaatatc actgtaattg gccaatgtcc
     7081 actggttagt taaatcactg tgcaagctgc acagaaccat aaaaagcagc ggcagcagta
     7141 aaaatgcaca gaagataaag gaggcaggaa ccagaaccaa gcgttttttc atctctttca
     7201 tcccctcatc aattcttgaa tgtcctgcat tttctctgac ggtgcaattc ctaataacca
     7261 gcaaaacaat ggagccgcca gcagctgtgg tacagtttgt tccttcagct tttggcgttt
     7321 atttgaaatc aaataaaaag gaacttcacg atgtgcagcc aagctgccgc catgcagtcc
     7381 gtgcgcatcc atcccgtgat ccgcagtcac cacgacttga taaccttttt gcagccattc
     7441 aggaaggtaa atagccaata atctgtccgc ctgattaacc gctgctgcat attcatgaga
     7501 atcggcggta aatttgtgac cgacatcatc aatattcatc gaatgtacga ccaaaaagtc
     7561 tggctgatac tgattcacta aataatgggc atccgcaaat aaatgcgaat cagggtaagt
     7621 gtcctcacta taaaagatgc catgctgaat cagtcgctcc tcctgatgct ggatgcgatc
     7681 aaccaaacga ttataggggg cttgattgta caactcgctg taccagtggt aagcggcggc
     7741 tcctgttttt ccgccagctt ccttaactaa ttcaaacagc gaccactctt tggatcgctg
     7801 agcatggtaa ttactataga tgccattttc ataggctgga acgccggtca tcagtacttc
     7861 gtataacgga cgtgaattgc tgggcagctc actttttacc ttcaataagc ttgcttgctg
     7921 gtgctcaact aaatgattca tgaacccaag ctgggcgacg gctgtgtcgt agcgacagcc
     7981 gtctagtata actacaatca gtttgtcaag tctactttgc ataggtcagt acctccgcct
     8041 gccaaagctc tggaagctct acagaggtct tgttccaagc ttcattgtct tcaactggca
     8101 ccgccgcttc ataagcatcc tccggcagta atttttcagc tacttcttta ggcagctcta
     8161 ccttgcgaat aggtcgcgca tagccctttg ccaaattgat ttgcccttca tcagataaaa
     8221 tatattcccg tgctagcttt gccgcattgg gatgaggggc atttttattg attaaggttg
     8281 tatacccgct aatgatggag ccgtctgttg gcacagttac ctcaaaacgc tcggagtcaa
     8341 tttggtcacg ataatttagt ccattgaagt cccaaacaac tgccacatcg atttcacctt
     8401 tttcaagatt tgctacgctg gcatcaacgg tggacaaacg gcctttttct gccatttttt
     8461 tgtagtaatt cagacctggc tgaatatcct tttcgtcacc tccgttggca atcgccgcag
     8521 ccaatacagc aaattgtgct tggttggcaa tcgtcacatc ccccacgcta acttgatagt
     8581 caccctcttg cagctccttc cagctagtcg gtgcttgctt cacattctct ttatcggtga
     8641 taaaggaaat ggttcccgta tagcttaaca accaatcccc atcatcatcc tttgcccaat
     8701 ccggaatttc ctcccaatag ttggtcttat aaggtaaggt taactctttg ctttcagcca
     8761 acggaccgaa gctgataccg acatcaccaa tatcggcggt tccatttttt ccttctgatt
     8821 caaacttagc caattcttct gcactggaca tatccgtatc cacgtgactt aagccgtatt
     8881 ctttttcaag gtcgttccaa gtgcctaccc aatttgccca agtatccggc attcccaagc
     8941 tggcaatctc tccttcttcc ttggcttttt ctataatcgt atctaaagag tcgtctgccg
     9001 cagccgattg cttcgttgct tcacctgagc cgcagcctgt cagcaggata cttactgttg
     9061 ctgcacaaaa taaaccgata gtcatgttac gaattttttc attcattttc attcattctt
     9121 ctcccctttc gataattcca ttctaacaaa gctttgtaat gagagaatca gtttcttgaa
     9181 aaggtttcat gaacaatgta aaggttgtat atggtttgta aatttcgacg aattcccgtt
     9241 aaccttctaa agcttttgcg gtatgatagt agaaaaaagg aggctcccta tgcagtatta
     9301 tgatcagcat ctccacacgt atttttcctt tgattcagag gaaaaatttg aaaactattt
     9361 agcctatcag ccggagtttt tcgtttctac ggatcacttt gatttaaaca atccatctac
     9421 cggaaaagat gacattccgg attatccagc ctatataaac aaactggact ctttaaaaga
     9481 aacctattcc accaaatttc tgcgaggtat cgagattggc gttgttccag gacaagaaga
     9541 gcggatcgtg gattacctcg ctcagcatcc ttatgattta aagctggtaa gtattcatca
     9601 aaatggccgc tttgattata tggacgatgt ggttttaacc aaagatccca ttcaaactac
     9661 caaggaatat tttgatcaga tgttgcaggt cttgacggat tttccagaag gagatatttt
     9721 gactcacttt gattatggat tacgccgctt caccttcaca gcggaagaat tcgcggagca
     9781 ttttgaggct cagctgctaa cgattttcaa aaaggttgtt gaacgagaat tggcgatgga
     9841 gttaaatgcc aagagcttct tgaaatacgg caacgaagac ctttaccgtt atgccgttcc
     9901 tttgtatcaa agtgtcggcg gaaagctctt taccttaggc tcggatgctc atgttgccga
     9961 agattatcag ctgggcttta aaaaaatgag tcagctgttg gcagactgtc aggtagaaaa
    10021 gttgctagtc attcaaggaa aagaacgccg cctgacgccg ctgccgattt tgtgattcct
    10081 atataataga agaaagtggt gaaaaaaatg agcaacctgt gggactacaa tcaagaggca
    10141 cccatccatt atttaattgc gcgacattgg gatgcgttga aaattgaggc ggtttgccga
    10201 tccttgttag cagccgttcc aaaacaacaa ctggaaaatt ttttggtggc tgacagcttg
    10261 cagcgggaaa aagttcaggc ttattttgca gcctttaaag atcagccttt ggaatatttg
    10321 catgcgcagt ttcatctttt ttatcaagta gccgccccag atgactacaa cgatttacga
    10381 ggacagctgc aattgacctt tcaagcggat gaaaccgcct ataccgtttt attgggaatg
    10441 gctcgccttg gtgatcaggc gaaggtcgag tggcggattt tcgatatttg atggcaggat
    10501 caaggcgttt cagttgcaaa ccttcctctc tttcaattag aaaaaccgca gcaggaagaa
    10561 gtttgtgtgc tggtcaccga tgctggtgaa attcgtctcc gcttatttcc tgaccaagct
    10621 cccctggctg tagccaactg gcgctcccta gctgagcaag gcgtttatga caacacacct
    10681 ttcggctcgt gtcattaagg aatttgttat tcaaggtggc gctttggatg gttctggtga
    10741 tgaagctgtt tccagctatg agggcttttt cgctgatgaa gtgcatcgcg gactttatca
    10801 ttttaatggc gccttggcct taggggacca cggcccccac accaacggca accagttttt
    10861 tattgtgcaa aacacaaagg cgcaggctga cctgctaatg taaagcaagc atacttggag
    10921 cttggaggac taccggagct ggatggtcgt tatactgttt ttggacaggt ataccaaggg
    10981 ttagaaatcg tagcagcgat cgcagctcag ccgacaaaca gtgaagatgt accgattgag
    11041 ccagtgttca ttcgacaaat taacttcgaa aaaacatcgt aaaaagacac tcactgactt
    11101 cagcaaaagc tctattcttg agccggctgc agctagtgag tgtctttcat ttatccataa
    11161 gataaagctt ggattcaata aataaataaa tgttttggtt aaagctgtga ccagcaaatg
    11221 aggaaccgtg cgagccagtt gatctgccaa tgcaaacatt tctttaggtg aaggcgctgg
    11281 cgtgtctgcc aacggatact ccagtcccaa tgcttggtaa ccgtgcatac catagcagtg
    11341 gtacggtagt aattctaacg gtacgaaccg atcaagatct gccacaaaac aagcaatcgt
    11401 cagcagctcg gtcaggttca tattgatacc tggaaccaca ggaactcgaa ttcgcacttc
    11461 gcagttagga tactcaatta aaaagcgacg aatattcttc aaaatttgtt ggttatctaa
    11521 gccggtccat tgtttgtgga gcttgctggt aaacattttt acatccacaa acaatgtatc
    11581 cacataggga gctacctttt ggatttcatt ataagcacca caaaagctgg tttccagtac
    11641 ggtattgatt ccaatgtatt tcgatttttg caaaatttct ttagcaaaat ccgcctgaac
    11701 cagtgcctcg cctccgctaa tggtcactcc gccatccgag tgaaagtaaa aaacagcatc
    11761 cttctcaatc tcatccatta cctcttcagc ggtcatggtc tggccaaatc ctttttcaat
    11821 ctgcttgctt tgtgattcgg gaatcgaaca ccaggcacag cgtaatgggc agccctttaa
    11881 ataaacaacg gtccgtaaac cagcaccatc atgtagagaa ccctgttcta ttcttaatac
    11941 tgttccttcc atatagattt ttgaattaca cactagttta aactaaaaca cacaaggaaa
    12001 aacttaccat ttaatcataa aattagctca tataaatata ctaggatgta aaatagactg
    12061 gtcaaaatct ggtcaaaaaa gaggactaat acaggtagtc ctcttttttg ttattttaat
    12121 attctaaatc attttttatt ggtttattcc cttattcgat gtagactcaa atcactatat
    12181 cactagatat caaacggcgt catacggaat caaaaacatc aaatttgacc tgttaaatgg
    12241 tcattgaata acattcaaaa tcaataaaac ggagtcagac acggagtcaa aaacgctgag
    12301 agtaaaaaat atatacagcc ttgtaggtaa agtaccacag ggctgtatat tttttatttc
    12361 agattacctt aaatgattcc atttactttt tatctcattt tttcctacta gttagtatca
    12421 aatactctcc tattttctca ataaactatt tttggataca tggtatctga aacagctaaa
    12481 accttgtcat tacagcaaaa aatcacgtta tccagactgt agacaaagtc cattttatgg
    12541 atgatgtcta cagtcttttc ttttataata gatttatgaa aacgaatgca aggagtgata
    12601 gctatgatgt ctaaaaacaa tacgaatggt agaaatcaat ttgcgatgct tacgattga
//
LOCUS       DACDYI010000081        12212 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.081, whole genome shotgun sequence.
ACCESSION   DACDYI010000081 DACDYI010000000
VERSION     DACDYI010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.081"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..137
                     /locus_tag="IWM52_002410"
     CDS             <1..137
                     /locus_tag="IWM52_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9974632.1"
                     /translation="QFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLVSMFTE
                     "
     gene            474..713
                     /locus_tag="IWM52_002411"
     CDS             474..713
                     /locus_tag="IWM52_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974633.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(1075..1335)
                     /locus_tag="IWM52_002412"
     CDS             complement(1075..1335)
                     /locus_tag="IWM52_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974634.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1520..2017)
                     /locus_tag="IWM52_002413"
     CDS             complement(1520..2017)
                     /locus_tag="IWM52_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAP9974635.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(2147..2857)
                     /gene="budA"
                     /locus_tag="IWM52_002414"
     CDS             complement(2147..2857)
                     /gene="budA"
                     /locus_tag="IWM52_002414"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAP9974636.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(2870..4534)
                     /gene="alsS"
                     /locus_tag="IWM52_002415"
     CDS             complement(2870..4534)
                     /gene="alsS"
                     /locus_tag="IWM52_002415"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAP9974637.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(4739..5401)
                     /locus_tag="IWM52_002416"
     CDS             complement(4739..5401)
                     /locus_tag="IWM52_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9974638.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(5411..6226)
                     /locus_tag="IWM52_002417"
     CDS             complement(5411..6226)
                     /locus_tag="IWM52_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9974639.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(6488..6931)
                     /locus_tag="IWM52_002418"
     CDS             complement(6488..6931)
                     /locus_tag="IWM52_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAP9974640.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(7065..7403)
                     /locus_tag="IWM52_002419"
     CDS             complement(7065..7403)
                     /locus_tag="IWM52_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9974641.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(7391..7768)
                     /locus_tag="IWM52_002420"
     CDS             complement(7391..7768)
                     /locus_tag="IWM52_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9974642.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            7992..9185
                     /locus_tag="IWM52_002421"
     CDS             7992..9185
                     /locus_tag="IWM52_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9974643.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(9351..9773)
                     /locus_tag="IWM52_002422"
     CDS             complement(9351..9773)
                     /locus_tag="IWM52_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9974644.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            10363..10818
                     /locus_tag="IWM52_002423"
     CDS             10363..10818
                     /locus_tag="IWM52_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974645.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
     gene            complement(10980..12152)
                     /locus_tag="IWM52_002424"
     CDS             complement(10980..12152)
                     /locus_tag="IWM52_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9974646.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
BASE COUNT         3716 a         2422 c         2023 g         4051 t
ORIGIN      
        1 agcagtttca aaatgaagaa tcactagaac gctttctagt cagcattttt gatacataca
       61 atcaaaaatt tctaaacaga agccataaag gttttcaaca ggtaaccgat acattagttt
      121 caatgtttac tgagtaacta attattttgc aggaggacaa tttatttaca caaaattatt
      181 gacgctccca gctatcctcc tagcaactat ttaaataccc aaaaaaatta cctctagata
      241 atatcatacc tacgttttca cttataaatt ttttcataaa aaaagcgttt agaggcataa
      301 tcgttaatac agatggtaac caatgtatct ggtttgaact gggatatcac cctctccata
      361 tatgtattac taagactaat agatactata aaagacttga tagttttgca ttgattttgc
      421 ctaatactga gcataggtgt atgctaaacg atactataag gagtgtgtac ataatgaaaa
      481 gaattctgtt tatcagttta tcaaatttat tgttacttac tgcttgtggg acttctgcat
      541 caaagaacat accttcagat acgcaaattt cgaatagtat cgattcaaca atttctacaa
      601 atgagaaaaa ttctccggta gaaacttccg atataaaagt ttctacctct gcaaaaaaca
      661 gggtaaagaa actgtcagca cgtctccttt atttcaaaca aactctgaat tagcttcttc
      721 atcttcttca gcaaataatg atgatagttt cgcgtttact cctaactaaa tgcaaccttt
      781 acaaatgctt tacgataaca agcctgagct aaatgatcct gatatcagtt ttgcattttt
      841 tgaaatgatt ggatcggatg atctttttaa aactactagt ttgtctatcc gaaaatagga
      901 cagttctggc agtataggtt tttatcgagt ttctccacca ggcctcgtta cagagacaga
      961 ttctctaggc gaaccttttg aataaatcaa aaatcatgaa caatcactgc caactaaata
     1021 aagtgccttc aaaagcttgg tcttctcgct tttgaaggca ctttatttaa atctttatga
     1081 aactaaagta tgttcatatt tgttgctata gttatataga tattctgttg tttccaactc
     1141 ataaacgtga gtatattttg ccactacttt tccgcatttt tctaatattt cattgcgatc
     1201 gacgtcatga tattcgtcta cgcagatcac aacacaattt tctaatttac tgattacttc
     1261 tccaaccact ttttgtttca agccaatagg ttggcaaata tatttttttc ctaaaatgat
     1321 agtttcgtta ttcatatttc atccatcctt aatgtttttc cattatttaa aatgataata
     1381 ccattaaaat atgattatga aaatcaataa cacttgtgat tttataaaaa aataaaaaaa
     1441 ccgaaaaaca gaaatgccca ctgtctttcc ggctcttttt attcttgatt tctcttcttt
     1501 tttgtttcaa tagacgattt tagttattaa taaatgaaaa agcatttata ttaaaatttg
     1561 ctaataaaag atctacatat ttttctagaa atgcttgatc gatttcatca taccctttcg
     1621 ttactccgca atcgatatct aaaacaccga ttaaattacc ttcttttacc attggcacta
     1681 cgatttctga ttgtgcggct gaatcgcagg aaatgtaatt tgcatgggtt ttgacatcat
     1741 caacgatcaa tgtttcttgt ttttcagctg attctccaca aactcctttt cccatcttga
     1801 tacgtgtgca agaaactcgc ccttggaacg gtcctaaaat caattcatta ccatcgaata
     1861 aataatatcc tgcaaagaca gtctcaggca aagtctcatt caacaatgca gatgaatttg
     1921 ctaaatttgc taaccagtta tcttctgctt ctaataatcc ttgttgctgt aataataaaa
     1981 tttcgtatgc atcttttttc tgtttttctg tccacatgat aaaaaccgct cctttttgat
     2041 ctatggagaa gattatagct cagttttttt gtaaacgcta ggtttcctcc tcaaaaaaac
     2101 gagatacgtc aggttaatca ccttgaacgc atctcgctgt ctttttctat tctgcttgtt
     2161 ggatttctgc tgctaaatca gcataatcaa tcgtttcgtg aacaaaagtc ggatcatcaa
     2221 ctggaaaatg ctgtacaagg gacgagatgc ggctaatctc aacttttcct tcagccactt
     2281 caaaatccat tacatgtccg ccaaatgtat gtgtatcatc gataaaatgc aagtgaaatc
     2341 ccgctgcagc aactccttgg aataattcag gcgtaaagaa cccgactaat gttccttgga
     2401 cattgctttg ttggaattct ggttgagact ctgaaatttt tgctagtctt ctgtaaggtt
     2461 tttcttgctt cggcatgata cggatgtgca tatttttgaa taatccagtt atcttcactg
     2521 ccaaaaatag gttttccccg gcttctctta ctaatatgtc ttttttcaat tgttctccat
     2581 ctgcagtttt tgagacagaa aaactagtat ctggcgtaaa atctgtaatt gctgcatagg
     2641 gggtaagttc acttccatct aattgggcaa ctgagccatc tgatcttcct tgataagcta
     2701 cgccatctaa aaagatgact tcaccatcaa gcccatgcaa tgttcccaat cccagagttc
     2761 ctttttctaa tatatgagca atcgtttctg ttccatccat caagccagcc atcaaagctc
     2821 ctaatgtccc gtgctggtat aagatttttt cagtcatgtc gacacttcct taatagaatt
     2881 gatctggtaa gattgtttct cccaatttct cattatcacg gtaatcaatc ggaatatcaa
     2941 taattacggg tccttctgtc gcaaatcctt cttctaaagc tttttctaat tcctctacac
     3001 ttgtagctcg gattccttta gcgccaaaag cttctgcata tttcacaaaa tcaactggac
     3061 caaagtctac acctgacgcc cgctgatatt tcattttttc ttggaactct accatattgt
     3121 agtgtccatc attccaaatc aaatgaacga tattcaattt tttacgtacc gccgtttcta
     3181 aatcctgggc agagaataaa aatcccccat cgccggaaac tgatacgatt tgtgtatttg
     3241 gacgcaccaa tgcagcagaa atcgcccatg gcaatgcaac accaagcgtt tgcataccat
     3301 tactgaataa caaatgacga ggttcataac ttctaaagtg gcgtgccatc caaatataat
     3361 gactacctac atctacggta acagtcatat catctgttac ttttgactga agcgtgttaa
     3421 taacttctaa aggatgcaag atacctgctt ctccttttga ttgaacaatg tcccgctccg
     3481 ttaattctgc ttgaagagat gctaaatatt cttttgcatc ttctgacact tgttgaggct
     3541 ctaatgaacc tgtcaataaa tcaagagtgg ctgagatatc accgatcaat tctctttcag
     3601 gctgcataaa aggatcgatt tcagctggcg cttcatcgat cacaatgatc cgcgcatctt
     3661 tttccgcatt ccagtttctt gcttcatatt cgattggatc atagccaata gcaataacta
     3721 gatcacttcg tttcaaaagc atatctcctg gttggtttct aaataaaccc acacgaccaa
     3781 agaaatgcgc ttctaattca cgtgagatca ctccagctgc ttggaatgtc tctacaacag
     3841 gcaatgctgt ctttccaact aattgcctga tggctaacgt ttctttttca cttgatcctc
     3901 gcatacctac taatagtacc ggtagttttg cttctctaat gcgttcagct aggtaacgga
     3961 tatcatctgc agaagcagaa cctaatttag gatcgctcaa tggcttgatg acattccctt
     4021 ggacaggtgc atccactaca tcttgcggga tactgataaa gctcgcgcct ttttttgaac
     4081 tcttagccat acgataagca ttggcaatca cttctgaaat tgtttctgga tcttggattt
     4141 ctgcactgta ttttgtgatt ggctggaaca gtgctgcatt atccatgctt tgatgggtca
     4201 acttcaatag atcgcttcgt tttacttgtc cggcaattgc taaaactggg tcaccttctg
     4261 ctgtcgctgt taccaaacct gtagctaagt tactagcacc aggaccactt gttgcgataa
     4321 ctactcctgg ctctcctgta atacgtccga ttgcttgtgc cataaatgca gcattttgtt
     4381 cgtgacgagt cacgattaat tcaggacctt gatcctctaa ttcattaaat acaccatcga
     4441 tttttgcacc tggtataccg aatacataat caacatgatg attaatcaaa ctttctacta
     4501 cagatttcga accttgtttc attttttcgt ccattgaaca tcgtcctttt tatgctttat
     4561 ttttaattat tagttagaaa taatcacaaa taattgatac agtttctaaa cctgtttcat
     4621 tcaaaagtat agcacgtttt atttcatttg tttagccata tgtttcaaaa aatgaaaaag
     4681 aatgacagtt tttacataaa aactgtcatt cttaattgaa agtttcaatt tataaagtct
     4741 acagatccat agttctcatc aatttacgat tgttgaagta aatcaaacca aatacgatag
     4801 aaggacctag tagaatacac cattggggct ttacgaaaaa gaagagaaac aaacttgtca
     4861 atagtaaaaa atctagtaaa ctcacatacc attttttcaa cgtatagtag aacgctatcc
     4921 gtagaacttg atttggtttc gacgctggat ttctcacctg aaaataccag tagttgatgc
     4981 taaaggaaac tgataaacaa gcaagtaaaa caaacaacgg aatcaaccat tttccataag
     5041 aaaaacgtat aaaaaagaaa caatcaaaaa tcattatcac actaactatc caagcaaata
     5101 gccaataaaa aaatcccttt agccaaaatt ggcgaaacgt ctttatgaac aaacgtactg
     5161 gtgaaaaact tttttctttt gctaattctt ctgtcatacc tatcatcacg atgcaacctg
     5221 gaccaataaa aagtaaagaa aaaataaagg aaaagacatt ccttccatct atcgacaaaa
     5281 ggaacgcagc tgctatatac aagatattga cacaccaaaa acaaaggcta cttgccaatg
     5341 attgatacaa ccaataaaag aattccatat aaacattttt gtcaaaacgc tgctctttca
     5401 taatcacctc ctattccttc attcctgatg aagcgatccc ttctacaaaa tatttttgca
     5461 tcgctaaaaa tataaaagca acaggaagaa cagaaacaac tgaggcagcc atgattaatg
     5521 cgtactctgc atcaaatagc ccaataaaca ttttcaaccc taactggatt gttttatttt
     5581 ccgtagaaga caaataaatg aatggcccca tataatcgtt ccatgtatta acgaatgtga
     5641 tgatcgtcaa actggcaatc gccggttttg taagaggtaa aatgatcttg cgatagattc
     5701 cccactctga taagccatcg atccgcgcac tctcacataa agaatctggg atactactgt
     5761 agtattgctt caacaagaac acaccaaaag cattgaacgc ttgtaatagc accaacgacc
     5821 acaacgtata tgttaaaccc acttttctca tcataatgaa ctgcgggatc atataagact
     5881 gccaagggac agcaatcgtg ccgatatatg ctaaaaataa aatatcgcgc cctttaaaat
     5941 ttagtttaga aaacccgtaa gcagcaaaac tagaagtaaa caactgaatc aacgtgatca
     6001 caacgcttaa aaaagctgta ttcttaaaaa atgtcactaa aggaatacga gaccaaataa
     6061 ccagataatt ctctaaatgc caagttttag ggatccattg aatcggaatc gtgaacactt
     6121 cattatttgc tttgaatgaa gcagaaacca tccataaaaa aggaatgatc gtcaataaag
     6181 ctaatacaat cagcagaatc atacccaacc attgaataaa gggcatgttt tttgaacgaa
     6241 tttttgcttt ttttctttca tttcgaacac ctccaaagtt ataatcttca tttctttgta
     6301 atgattacaa tccagaacaa cggttattat agctgattca tttccgttct tcttatgttt
     6361 gtaagcgctt atttaacaag ttatgtaaat ctctttattt tttttgattt aaaaatgaaa
     6421 aaatacaaaa gcaagactgt catacgtctc gcttttgtat tgaaatttct tcgattagag
     6481 atacttatta ctgttcaaaa gcttctttga aacggtgaat caattcattg actacaggtg
     6541 ataaaatccg attctttttc cagactagca tgcagttcgt ttcaatcatt gggtcaagcg
     6601 gaacgaatgt cactcgcttt gaaagatgac ttacagcacc ttctatgact aaagcagcac
     6661 ctacctgatt ctcaattagc gcaaatacat tgaaaatcaa attgtacgtt cctgtgatca
     6721 gcaactgctc cttttttatg cctaaccagt cagcaaacat tcgctgtatt tctggacgct
     6781 ttgagatcaa aagaggtaca ttcgcaaagt cttcttttgt tatcacgtct ttttttgcta
     6841 aaacagaatt ttctaaaatg agcaatcccc atttttcctt tcttggtaac aaaatcgttt
     6901 cgtatttctc tgtatctact ggatctagca aaatcgctaa atctaacagg cttttttcta
     6961 gtcactccgt gatatcatca cttgttccgc tgtggatatg aaattgaacg ctgggataat
     7021 cacggatcaa cttttctagt aatagtgcca atgtattcga attatcagct tccacacagc
     7081 cgatcgtaaa tcggccgctg aataattgtt tttttctgtt ctcgaattct tgagtggttt
     7141 gattcgttaa atctaaaatt tcttctgctc ggctttttta gaaaataacc tgcttctgtc
     7201 aagatcattt tgcggttttc gcgtacaaac agttctgttc ctagttcctc ttctaactcc
     7261 ttcagttgtc ggcttaaggt tggttgtgta agatgtagaa cttctgcggc ttgagagatt
     7321 gttccttctt ctgcaattgt ccaaaaatat tttagtaagc gtaactccat agcgatgctc
     7381 cttgattttt ttacttgata cattccattt tatttctaga taacacacga tcatatttct
     7441 tgatttttcc ttgtaaacgt tccaaacatt cttgcatttc agcaattttt ttcgtcaaaa
     7501 gttcactttc ttcaatcaaa agcttacgcc gctcaaatac agttgcttct ccttcgctag
     7561 ataggcgaat atattcaatc aaagactcga tcgataatcc ggcatttcgc atacagatcg
     7621 taaagtcgac ccattttagg tcttcttctg tataatcacg gattccgcct tttgtccgat
     7681 taactggtgg aatcaacccg attcgttcat aatagcgcaa tgtatcggcc gaaatgccct
     7741 tttcttcact gacttgtttg atattcatct gatcactctt tctcttaatt tcttgccact
     7801 ttacttatgt attttattta ttctacaact ttaagtgtac tccaagtcaa tactcatttc
     7861 tattaaaact aagctggatt atctatatcg gttgtttgtg tattatttca aataaaaaag
     7921 tcttgttttt ttattctttt ttggatatac ttttaacaat ttagggaaat tttaggaaaa
     7981 ggggtataaa aatgacaacg tatcagcgaa agttattatt atcgacatcg gcgggaattg
     8041 ctttagagaa tatggatatt atgtttttag ctttttcatt atcttcaatg attacttctt
     8101 ttcatattag tggtacgcaa gcgggtttca tttcaacgat cactaatttg gggatgctca
     8161 ttggaggtat tttttttggc atcatagctg ataaatatgg acgtgtgaaa gtattttctc
     8221 aaacggtcct tctgttttcg attgcttctt tattgatgta ttttgcttca aacatctact
     8281 tggtttatct ctttcgcttt atcgcaggtg taggagcggg aggagaatac ggggcgtgta
     8341 tgtccttgat ttccgaaagt ttttcaaaaa aacaaattgg acgagcttct tcggttgctg
     8401 gtattggtgc gcaggtcggt gcggctttgg ctgcgatttt agctgctgtg atcattccaa
     8461 cgtttggttg gaagatgttg tatgtcgtag gtgtactgcc ggtgttgatg gtcttgttca
     8521 ttcgtcgcgg cttgactgaa ccaaaggaat tcaaatctgt acaatctgcc gaaaagaaaa
     8581 ccaaacttag cagactgttt gaatcaaaac atcttgcttg gcaaacatgt ggccttagta
     8641 tcatggtaac tgtccaaatt gccggctatt ttgggttaat gaattggctg ccttcaatta
     8701 tgcaaaatcg attagggctt tcggtttctg ggtcttcatt gtggatggtc agtactattt
     8761 taggcatgtc tttggggatg atgacttttg gcataatcat ggataaattg gggcctaaac
     8821 tagccttttc tttgttttta atttgttcat ccttatctgt ctatctactt gtgttagccc
     8881 atagccagtg gagtttggta gcagccgctg tgatagtcgg ctactttatc aatggcatgt
     8941 atggtggata tggcgcagtg attagtagtt tgtatccaac agaaatccgc gcgacggcga
     9001 ataatttcat tatgaacctt ggacgagctg ttggtggttt ttcttctatc gtcatcggtt
     9061 tcttgatgga tcattatagt ttaactgccg taatcttgtt tttgagcggt atttacctag
     9121 tcagtctgct ggtcttgaca actcttactg gtgttacagc attaagggaa agcttgcagc
     9181 attgatagtt gagtctgtaa atgacaagaa agaggtgtgg caaaagcttg catctgcggt
     9241 aataagacca gccaaatcaa aaacatgaga cactgtttcc gtttggctgg tcgtattact
     9301 tgatgcaggg cagctttttc aactgctaat ttccttctat ctggcggcta ctattctttg
     9361 acgtccgtca tttcaaagta ctttactaat aacttagcat aacgaaaaac tgtggataca
     9421 ctcgtatatt gagcgtctgc ataatcttcc aaatttttct caggatgttg atacatgaat
     9481 tcaatcaaat gataattata tgattttctg tagaaatgaa gataaataat gtagattggg
     9541 aagttctcct gcttttccat agaaaagcgc cgattattct cattcttcaa aatgacctta
     9601 tcaggatatt tttggaacaa ttcttctaaa tctgttagat aattcaagat agacttactt
     9661 gataccttca attcgttttg tagttcttct attgaacacc aacgttctgt ttcaagcagt
     9721 aaatgaatca ttttaatttt cttttgagaa acttttgtta aaaaagtctg catattttct
     9781 cctcctgtat ctactttact atactacata caagaaaaaa aacggataca aatatcatat
     9841 tgtcacaccg tcaatagtgc ataaacctgt tattatttct ctttttttcc taagtattct
     9901 attgatatga atcatttaat cgtttattat ataaatgtca ataatctaat aataaaataa
     9961 cgtacatatt ttattattgc atacttaata acagaactat agcaaaaaat cccgtgtatc
    10021 aaaacgggat tagattaagt ttcctcaatc actattcata acacaattat tataacaagt
    10081 aagtaaacaa ttgaaaagga catttattga aaacggttgt tttttactta aggaggaaat
    10141 ttagtgaaaa aattagttgc attgtttttg gtcgcaggaa gtttgttgag tgggtgtggt
    10201 gctactacgt ctactactca aagcacaagt tcagctcagg cttccagcag cagtaccacg
    10261 atctccgaaa gtacggcaga ttcatcgaaa gaacaagaac aaattgaaaa gaaaaaagaa
    10321 gaagagaaac aaaaagcgga aaaagctgca ctagaaaaaa aagtgacaga agcggaatct
    10381 gctttgtcac aagctgaagc aaatcctaca aaagaaaact atgcaactgc ttcagcagct
    10441 atccaagcgc tacctgaaaa caaacaagag ttgacaagcc gtcttgctgc aattgattcc
    10501 acaatcaaag caaaagaagc agaagaagaa cggaaaaagc aagaagaaat tgcacgacaa
    10561 caagcagaac aagcgcaaca agaagcaatc gcacagcaac aagcagaaca ggcgcgacag
    10621 caagcagcag ctgaacaggc ctctcagcag gcacaagccg cagctcctga gcaaaataat
    10681 gaacaaactg tctatgtcac accgaccggt tcaaaatacc atacacataa atgtggaaat
    10741 ggtacgtata cccctgccac tttaagcgaa gctcaagctc gtggtttaac cccttgctcc
    10801 aaatgttatg gaaactaatt caaaagttat actataataa taaaattctt ctgaaacata
    10861 aaaaaataga agctggcgtt ccagatagta aaccaaaaat agcagtttac acgtccattt
    10921 cgtcagcttc tctctttttt catttagtct gttccctctg cttttttgga catcttcatt
    10981 cattctttct caatcaaata gtacacctct ttttcttttc gcagtttata tcttcctttt
    11041 ttcaatttca ttgaaaactg tttcgaatgt ttatgtttta catggatttt aagcaggagc
    11101 tcctcatgtt gcaagcgata gttcctgcct tttaaatcag aaaatctcac tagtccgtat
    11161 tcatcagaag ctttcacaaa tggaactttt tctctagtaa tatagtgttt gccgttgagc
    11221 gtttttacag aaaagaccat tccttcgatt ggtcgtccgt ttgtgttctt tacataaaaa
    11281 gataaaggat agtttcgctt agcgatggcc aaagaatcta aataagcgat gagtagccaa
    11341 actaagccga ttgatccaag tagtataccg ataaaccaaa taaaaaattt ccattttttc
    11401 cattgatcaa ttccttggat cgcttttttt gcctcttttg cttgataagg aatacgatgc
    11461 cccgtcacaa gcagtcgatg tgtattgacc atataaggag tacaggtaag caaagtcacg
    11521 agatctctgc ctttttggat acccaaagaa tctacttcat caggtaagac cacttggatt
    11581 ttttctactt gatacgccaa tgtctttcca ttgatctgga tgaaaaactg gtctcctttt
    11641 ttcaacttag gcaaatctgt aaataatttt gcttctggga gtccacgatg cccagaaaga
    11701 acggcatggg tgtttttccc gccagtcgga taagaacttt cttccaataa tgacgttcct
    11761 ttttgcaaaa atatttccgt tgtctgatcg aatataggta agctgacatt gatttttggg
    11821 atttcgatga ctccaagcat atgttccata aaaaaggcat tactaggtaa atcttttaat
    11881 gcttttgcat caactgcttg gttgaaactg cctatccctg gtgatacgtt ttgctctgcc
    11941 aactgttggt ttttctttgt cattttttcc tgctgttttt ttatttcctc tgaattctta
    12001 ttcgatgctt gcttttgata atggatgatc atttgctggg caagaaaatc attcagtgaa
    12061 tcttctacaa aaggataaga aaagatcccg atcccaacga ctaaaatgat tgccatgaag
    12121 atgtccagta ttcttattct gcttttattc atcatgtcca cctattttct aatatgtata
    12181 tgaagaggtt gtgaaatcag cgccttgacc tg
//
LOCUS       DACDYI010000082        12018 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.082, whole genome shotgun sequence.
ACCESSION   DACDYI010000082 DACDYI010000000
VERSION     DACDYI010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.082"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(17..598)
                     /locus_tag="IWM52_002425"
     CDS             complement(17..598)
                     /locus_tag="IWM52_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9974647.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(781..1407)
                     /locus_tag="IWM52_002426"
     CDS             complement(781..1407)
                     /locus_tag="IWM52_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9974648.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LIESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(1429..2760)
                     /locus_tag="IWM52_002427"
     CDS             complement(1429..2760)
                     /locus_tag="IWM52_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAP9974649.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(2915..3316)
                     /locus_tag="IWM52_002428"
     CDS             complement(2915..3316)
                     /locus_tag="IWM52_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP9974650.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(3402..3608)
                     /locus_tag="IWM52_002429"
     CDS             complement(3402..3608)
                     /locus_tag="IWM52_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974651.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(3703..5205)
                     /locus_tag="IWM52_002430"
     CDS             complement(3703..5205)
                     /locus_tag="IWM52_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAP9974652.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(5223..5678)
                     /gene="argR"
                     /locus_tag="IWM52_002431"
     CDS             complement(5223..5678)
                     /gene="argR"
                     /locus_tag="IWM52_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP9974653.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(5776..6393)
                     /locus_tag="IWM52_002432"
     CDS             complement(5776..6393)
                     /locus_tag="IWM52_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974654.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6614..7987
                     /locus_tag="IWM52_002433"
     CDS             6614..7987
                     /locus_tag="IWM52_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9974655.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            8062..9174
                     /locus_tag="IWM52_002434"
     CDS             8062..9174
                     /locus_tag="IWM52_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAP9974656.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(9246..10883)
                     /locus_tag="IWM52_002435"
     CDS             complement(9246..10883)
                     /locus_tag="IWM52_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAP9974657.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLLDEAKESLENLIQKLN"
     gene            complement(11051..11659)
                     /locus_tag="IWM52_002436"
     CDS             complement(11051..11659)
                     /locus_tag="IWM52_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9974658.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3215 a         2507 c         2151 g         4145 t
ORIGIN      
        1 tcacaacctc taattattaa agaacctttt gcgaagttaa caatctttct ttgatcaatt
       61 gaaaattctg cctaagtagt gatagatcct gtgtgacttt caacaataag atacttccct
      121 ggatttcagc aatcaacgct ttggcttgac ggtctgcttc ttcatcggac agtttttctt
      181 cagaaagtgc caggatattt tgtttcgtat acttgaccca ttgattgact aattctagta
      241 gcaacagacg gtattcttca tccactgtac tcaactcaat aatcaaatta cctacaggac
      301 agccatgata agtgatctgt gtctcatgat cagcgaaaat ccagtccaac atttcttcta
      361 atgcttcttc tggctgtttt ccagcattta agatgtcttc gaacaattct gtctgccata
      421 cgtccatatg cgcatggatc accgctagac aaacttcttt ttttgaagaa aagtaataat
      481 aaaattgacc ttttcctgta tcagaagctg ctagaatatc tcggattgac gtttcatgat
      541 agcctttttt ataaataaga tctttggtcg cttccaagat cttttccttt gttttcaatg
      601 atattctcct ttttcatttc tcatagtcct atattataca gtaaattcat cattttttct
      661 tcttaatcgt ttaaaaaaga ctgtgccaaa ttatctgaca cagccctcac tataaaactg
      721 ttctcatata gctagatgtt cattgattca agaactgcga ggacaaaagc aattcctatc
      781 ttatgatagt tgctctaata cttcctctgt tgtcatcact tgatctgcaa taaagccaaa
      841 gttcgtcaac gctgcatcca ttgcttcttg gccaggagca ccaattgcat ctgtcgctac
      901 cacaacattg aatccactct ctatcaattc acgcatatgg gaatcgctac acaagtttcc
      961 gttcattcca ccaagaatta ctgtgtcgat tccatttttt ctcaattgga gcgccaaatc
     1021 attgctttct ggtccaaaga ttttatgagg agagctgatg atcgtatttt catccaaata
     1081 aggtttcaat tcttcgataa aatcggctcc tgaaccttct tccactgatt catattgact
     1141 ttttctagca aacatctgct gctcaagcat catatgttct cctgcaccgc caaattgcca
     1201 tttatggtca tgtgggtaaa aataatgagg agaaataaag agtttgtatc ctttttcttt
     1261 tgcttgtttt accatttcaa tcatattttc gatcgtattg tatttttcta agatatcttt
     1321 tgtcaaacca aagccaccgc ctgctggttt caagaactcg acttgcgggt cagtgagaac
     1381 tactgctgta tttttattat tgaacatggt ggattctcca ttcgttattt attttaatac
     1441 atcatttaga gcttcactca ttgtcggatg agaatagatt tgatctctca ataacgtata
     1501 aggaagtttt ccattcatag ctagcgaaat caagttgatg acttcatgag aatcttccgc
     1561 atagatactt gcccctagaa tcaagtcagt ttcagggtcg actaaaactt tgaatacacc
     1621 tttaggatct tctaatactt gtgctttagg gacaccgctt gtcatatact tgaataattt
     1681 gtaattgacg ccttgtgctt tggcttgttt ttctgttaaa cctacattgg ataaagtcgg
     1741 agtgataaat acactgtaag gcacagcttt ccggttattc gttgttcgtt tattctcgcc
     1801 cttcaactga tccataatga ttcgataatc atctagagag atataagtga actgcaatcc
     1861 gcctttgacg tctccaatcg cccaaatgtt gtctgcatct gtacgaaggt attcatccac
     1921 agcgattgct ccgcgatctg ttagtttaac atctgtattt tctaatccaa gtgatgctgt
     1981 acttggtttt cttccagttg cagccaaaac ttttgacgct tggattgtta cttcttggcc
     2041 atcttctgta aaggtgacgg cagctgttga ttcttggtct gctacttgat ctacagatac
     2101 acctagatgg aattcgattc ccgcatttgt catgtcttct aaaatcattt gcgcgatatc
     2161 ttcatcttca cgtggaagaa attcagaatg ggcatcaaga acgacaactt tagatccgta
     2221 ttcattgaac attgatgcaa attccaagcc aatatatcca gccccgatga tcactaattt
     2281 ttccggtaat tgttcctgat ccatcgcttg cgtagaatca atcaagtagc gacttgtatt
     2341 caatcctttg attggtaaaa tgattggttc agaaccagta ttgatgaaaa tccgttcgcc
     2401 ttcaacaatc actttttcac cattattcgt cacttcgatt tgatggttag aaagaaaacg
     2461 tgctgtaccg tccatcactg tcccagttgc ctcatcgctg atcatatgat aatttttggc
     2521 gcgaagcatg cccgtcaatt tttctttccg agcaacagcc tctgtaaagt cgattcctct
     2581 ttctccattg aaaatcaatg atttagaagg aatacagccg atgttgatac atgttccgcc
     2641 atacatacgt gttgactttt caacgactaa aaccgattca ccttttgaag ctaacgcctt
     2701 cgctaaagtc ttaccgccct ttccaaaacc gatcacaata ttttgatatt tttccatcat
     2761 ttttccctcg cttctttttt acctaccgat tggtacaaaa atatgataac cgataattgt
     2821 ctattctcca aatcatctgc tttatttgtc aaaaaaaacc agacaaaaag cggtcctttc
     2881 acgtttttag tctggttcga tttcctttat tcttctaaac aatcgttact tctacatcga
     2941 tatttcctct agttgctttg gagtaagggc agaattcatg cgcatcttct gcaatttttt
     3001 gggcttcttc ttcgctgatg ccatcgatat gcacttctaa tactacaccg atctgatagc
     3061 cgccatcatc taaattataa agagagacac gtgcttttac tgtggactct gctgtcattc
     3121 ctgcttcttt gatcatatgt tgtaccgccc cattgaaaca agaagcataa cctgctgcaa
     3181 ataattgttc tggatttgtt ccttctgcat ggattcctgg aggagtgatt ttcatatgca
     3241 tcgagccatt tggtgcctcg acttctccat tgcgcccgcc atggttgatc attgctgttt
     3301 cgtatgcttt tttcattgta ctgctccttt cgattgacca tttgctactt ggctataatt
     3361 atagtgtatg acaagtaagg cgctttctac aaaaattctg cttattcgaa taccagaatg
     3421 ccgtcttcta ctatttttcc aaaaaacaaa aaaatactac tcgtgaacaa cagcgtcata
     3481 tttgttccaa aataaatagc aatcttttct acataatctt ttatatgatt gattttaaga
     3541 tacatatata ccagaaagtt gaccccataa accaatactg ttaaaaaaat aaccatattc
     3601 gttaccattt tttcctcctt ttgctggtct ccctaccagc actcgattta accataccac
     3661 tttttttgcc gtccaagcaa ctagcctttc aaaaagactg gtttattctg ctgttaatga
     3721 ttcaagatca gaaaaatgtc cgaataattt tgctaatttt actaacgcca acttgttctt
     3781 tccgaattca tcattttcta tctgtaatac tagtaacggc tcatgtaagc ttaaacggat
     3841 caaaaaccag ccgtttccgt atggctcttc tagatcatat cgaatacctt cttcattttc
     3901 cgggtcgaag ttcatccctt tttcttcttc caacagtcgc ttcatttctt ctaatatctt
     3961 gtttccttgt tcgcgtggtt catcgctgaa caattttaat cggatttctt gtacttctac
     4021 gggctgtttc aactctttga tttgttcatc taatgtgata ccttttcttt ttaattctgg
     4081 caaaagcatc aggatctttg cgatgacata tgcaccatca tccagaaaat agttttcctt
     4141 gaatgcagcg tgtccgcttg tttcgatggc tagctgtgta tctttcccaa gacggttaag
     4201 ttctaatgct ttattgatta catttcgata gccggaaata taacgaactt ggatgcctcc
     4261 tttttcttca atgaatcttt gtagatgaga agaagtaggg gaattcgtca cgattcttgt
     4321 tcctggatgc tcttttaata cgattgtact taatactgcg atcaaattat ttcgattgat
     4381 cactgttcca gaagcactga caactgcaga acgatccaca tctgtatcga aaatgatccc
     4441 aagatctgct tcatttgcta aaacagcctg cttgatactt tccatcgctt ctttattatc
     4501 aggatttggg atatgattag gaaatcggcc atctggttcc aaaaattggg aacctgttga
     4561 atccgcacct aagctattca atactttttc agcaaagaat ccaccagcac catttccggc
     4621 atcaacaatg attttccaac catttagtgg tttgctttct agtgttccaa tccccttttt
     4681 aatttttgtg actaaatctt gcgcataagg tgtcaaaagg tctgcttttt ctacgcttcc
     4741 agtttgttcg ctttttattt tttgctgctg tgataggata aaagcaatat cttctttttc
     4801 tgctcctcct tctgaggtaa agaattttat tccattataa taatagggaa gatggctagc
     4861 agtcagcata accgcagcat cgcacgagaa ctgttcaaat tgagtggaca taaacatagc
     4921 aggcgtagta gctaattcga aatcgatgac atgtatccct tgttctttca gactttggat
     4981 caacgcctct ttcagtgctg gtccgcttat cctactgtca tgaccaatcc caatcgtcca
     5041 ttttttattt ggatcggttg atttcttctt tttctttatc cagtttgcta gtccccagcc
     5101 gatctttttt accatgtctg tcgtcaagtt tacttcatat ccttcaactg gtaatgcgat
     5161 tcctcgaata tccgatccat tttgcagcgc tttcatatct ttcatataaa agtcctcctc
     5221 atttattccc tcattttttc cagcttttct gcaaatcgct gtgcttcttc ttctgagcgg
     5281 caaataataa tgaatgtatc tacaccggcc atcgttccga caacttcagg gtaagccact
     5341 tcatctagcc aattcgttac cacatcggca ccatttcgtt cagtcaatac gataaccatg
     5401 aactgaacag actggatacg caaaacctcg cgtctaattg ctgtacgcaa acgttcttca
     5461 tttaaagaaa cagatggctg gttaaataat gtatagcgta ctattttgtt ttcattttga
     5521 ctttttacta acttcatttc tcgaatgtct cgagaaatcg ttgcttgagt cacatcggcg
     5581 cctgcttcat gcagccgcgc aagcagctct tcctgtgtcc caataggttg ttctgtaatg
     5641 atctggcgga tcagtgcttg tcgctgtttc tttttcatat tagacacctc tcaatgttga
     5701 ttatagcaaa agataagtaa tatttcttca tattttggta ttttttactt ttattttttg
     5761 ttttcattca ggttgctaac ctttttctat tttaacacgc aaaatcgtct tcattttttc
     5821 aggtgctgat tttcttggat cgctgatgta aatctctttg tgttctttag aagctctttc
     5881 atagccttct tttgcgatga atgcttctaa tttctcaaac gttgcaggtt cttcggcata
     5941 gggcccgacg tgtaagattt gggcacaaag accttcttcc atctttttga atactacttg
     6001 ttctttcaag tcttccggaa gtttgctaga acgctgaatg gcttcttgtg ctatcgcttc
     6061 tgtaacaaag tcaggctgtc ggatcatcaa ttgataagaa aaatgttctt ttttcatcac
     6121 tggttcatgc aaatactttt cttgcagtcc ccattgtcct tccagtggat agacagtata
     6181 catctgataa tcaggaatca gccaattttt tttaggcgac atgcgtattg catagctgac
     6241 tgcatataga caggctactc ttcgtttaaa gtcttcctga ttcgggtcgc cgatccctct
     6301 gatgctaaag aatggttttt taggaacctc tagtatttgc gggctttttt tcatactata
     6361 gctcttgtct ttacgccatt caattttttc catttttctt cctccgcgaa attccattta
     6421 gttatttatt atcatacctc tttttgagtg acttggctaa tcgtgttgac actatttttc
     6481 agtttgtgct agaataagtg aagtaaataa atcgtacaga taacacgttt cggcgtgtcg
     6541 agagagaggc ggtttctatt tttataatag aaatcactct ctttttttac gaaatttttt
     6601 agaggtgatg taaatgacaa aacaaaaaca gctgctttta gtacctgcag taattgcaac
     6661 agggatgatg tcttttgcgg gcgtattgat cgagacggcc atgaatgtaa ctttcccaac
     6721 tcttatcaga cagtttgatt taacgactgc acaagtccaa tgggtaacaa cgatttatct
     6781 tttgatgatt tcgatcattg ttccgctgtc tacttatttg aatcgcaatt ttagtctcaa
     6841 aaaactcttt ttagcttcaa atctgctttt cttggcaggt ataatgatcg atttcttctc
     6901 tcctaccttc atggtcctat tgtttggtcg attgctccaa ggtgcagcga caggtatcgc
     6961 tcttccgttg atgttccata ttatcttgac atttgccccc ctcaatcgac gaggcgcgat
     7021 gatgggtgtc ggtactttga caacggccat tgctccagca attggcccca cttatggagg
     7081 cattatgact tcacatttta catggaatca tatcttcctg tttcttgtgc ccgttttgat
     7141 tctttctttg attatcggct ggtttgctat tcccgagatg gcagtacaaa aaagtggtta
     7201 tttggattgg ataagcgttt taggaatttg tcttttgttt agtggaagtc taacattttt
     7261 gagtacgttg ggaacagttt ccagctggat tgctttaatc atcggcggta ttggctggtt
     7321 ccttttttac tatcgaagta aaaaagtgga gcatccactg atccgactgt ctattctttc
     7381 aaataaaaca ttccgcttgt ttttgattgg cttcttagtt tatcagtttt tattacttgg
     7441 tgtatctttt gtcttgccga atttccttca gatcgttcaa ggaagtagtc cttttgtcgc
     7501 tggattagcg atgcttcccg gcgcagcggt tggtgctttg ctttctcctt tttccgggaa
     7561 aatgctggat caatatggtc cgaagaagcc aatttttgct gggctgattc tttctttgat
     7621 tggttggcta gcattattcc tactcatagg acacgcttca gtgggtgtat tgattgcttg
     7681 tcatgtgttt tatatgatcg gtatcggatt atcttacagc aatatgatga ccaccggaat
     7741 gaatgcactg aatgaagaac tacaaggaga cgggaacgca atgttcaata ccttgcagca
     7801 gttttctggc gctgtggcta cttcattagt agcggtcatt atcaattacg tccagcatca
     7861 tacttcccat aactatgaag tgtctactac attaggatca aaagcagcat taggcgtctt
     7921 gttgctgttg cttctcgtaa gctttgctcg attcgcctat tatctgttct ttgctaaaaa
     7981 agcgtagatt ccatatacgg caggtgttgc atctgccgtt tttttgtgtg aaaatggttg
     8041 tttgtaaaag gaggcttttt tatggaaaaa attgccatta tcggtggcgg gatcattggt
     8101 atgaccctag ccagttactt agatacatca aaattcaatg ttgctttatt tgatgaaggg
     8161 acaggacagg caactaaagc tagcgctggg attatctccc cttggttgtc gaaacgcaga
     8221 aacaaacaat ggtatcgtct ggctaaagac ggcgctgctt ttttccctaa acttataaaa
     8281 gatttccaac ttgatcaaac gatttacgca caatcaggga cacttttgct tcgacctgaa
     8341 aaagatttag ctgaattggc tgttcttgcc gagaaacgaa aagaagaagc gccagaaatt
     8401 ggaaacatcg aatggctttc acctgaacag acatctcaag cacttccgtt attgaaacca
     8461 gcagtttcac tgcgtatcag tggtggtggt cgtctggatg gaaaagcata tttagaacat
     8521 cttggtcagc gtttagcaca aaaacaaatg gagcagatcc aggcaaaagt cactttgcat
     8581 aagaagagcg attcttggct catcaaggga ccgtttggag aatacacagc tgatcgtgta
     8641 attttgacac ctggcccggc acttaagcct ttattacaag aattaggcta tgctgcagat
     8701 atacgtccgc aaaaagggca gctcttagca tttcagacat cttataagat gagtcaagac
     8761 tggcctgttg ctatgcttga cggagaagct gatttcattc ctttcatgga tggaaagatc
     8821 cttttaggtg ctacccatga aaacgatcaa ggctgggatt tggctcctac tagcgaagct
     8881 tataagggat tgactaagag tacagaacag ttcttgactg atccagatca gataaaaaac
     8941 tggccatacg agttccgtgt aggtacccgt gcttatacgt cagatttttc cccatttttt
     9001 ggcgtactgc cagatgatcc aagcttactg gctgctagcg gtctaggctc ttctggcttg
     9061 accacaggtc cttatatcgg ctatctatta gccaacttct taaatactgg tcattggtct
     9121 acggaagatt atcaaaagga catctccacc tatattacga aaaatccatc atagaaaaac
     9181 agctttttca agtaaggatc ccgagcgata ttgcgcatgg attacttctt gaaaaagctg
     9241 ttattttagt tcaatttctg aatcaaattc tccaaacttt cttttgcttc atctagcaag
     9301 agatcttttc ctatctgagc ttcaatgaat cgtaaacgaa tatcaagtcg ttgtttcacc
     9361 agatttttat aggacgtatc ctcaaaagaa acaggaaaat ctggtatagg taaatagtgg
     9421 atgtcttcaa atggaccaaa cgacttgaat tctgccgttt gttgcacgat tctttcaacg
     9481 attgacaatg tcgtttcagg tttgattttt ttatcatcga aatatccagt attcaggaca
     9541 tagcaggctg tttgctgttt caaaaacaac tctcgaaaag cttgatagtc ttccctaaga
     9601 ggatagacac ggaaaggatt agcaaatggt tcaatgacta gctcatctag ctgtcttttt
     9661 gtagtgatat tttccgcgtc tgatcgtttt gttgctaatg ttgcaccaaa agacgctgct
     9721 aaaactgggt cattgatctt gataattggc ggaagcgaat catctttcat gatccaaaaa
     9781 acagcatcga tcttttcgct aagatgatcg actcggtcgg gtgtcgcata acgtgatttg
     9841 actgtccgcc catttccgtt tcgtatatct cctgttacta ggactttttc tcctttactt
     9901 gtacatgtca cgcccacatt ctgacaagta aggaaagaag aaatggccgg atcatcagac
     9961 ggatagtctt gtgttttatc aaagtaagaa ggttccaagg cagtagcaaa accatttttt
    10021 cgagaaataa cgaaagcatc gtcatgcaag atcgaaacag agtgaccttc cgtatgtttt
    10081 gctaaggtaa tcgttgattt tccagaaccg gacaacccaa atacagccat cgtataggtc
    10141 tggtcattac tagcatattg tttcatccca ccgtggcagg caataaagcc attgcgattt
    10201 cccaatgccc aagcaagcgt caaagtcgct ttttttaatt ctccaaagta acgtaatcct
    10261 agcacagccg ctgtattatg gagagcatca aacaaaatga gtccatttgg aaatgtttca
    10321 tgtgtccatt cgggatcaca aaagatataa agatctccct catcaaagga aacagacttt
    10381 tgataagtct ctaagatctt cgaattcgct ggctgaaaat tcagcaaata agaatacaaa
    10441 ttgttctcaa atccttctgg aatcagcaaa tgactttgca cagaaaattc ttcagatagg
    10501 ccaacgatta cttttgtatg gtaaaactgg cgcttgcgac tttgatacac agcttctctt
    10561 aaaattttct cgtatttgct ttgttccttg tctaagtcct caaagatccg ccgagcactt
    10621 gctgttcttc ccacaatgct tccatcattg aatactagtg tcctaacatc agctggtaaa
    10681 tctagttctt cagcatgtga gattgttaaa tctgtgatga tcgttcctgg actccctttt
    10741 gctagttgat aagctgtctt cctgtcaaaa acttcatgaa cattattacc gtaaaatgtc
    10801 gtttctacaa tcgatcgaat actggataac agtggatttc cacttgtgat ttcgtttaat
    10861 tcgagactct cttttgtact catggctcct cctcattccc taaacaaaaa aacgttttct
    10921 ttattataca ttattcgagt ttttatttgt actgctgcgc tgtgggaagc aaacgagtta
    10981 tttttcacac aaaaaaaccg agaacttgaa taaacaagtt tctcggtttc atcattttcc
    11041 ttttatcggc ttagtaccag ccgtttgcta accagaatgt tttagcagca tcccatgaac
    11101 cataacgaga tgttacatat tgttctgcta cacgttcttg attggcagct gaatagtcgc
    11161 cgtttaagta tgaagcatct aattgataac ggccgatgta acgtccgtta gtagctgtgt
    11221 atgaaccgcc tgattctttt tgtgcgatcc attcttttgc agagcttgaa gttggcgctg
    11281 ttgcagcagc tggggcagca ggagctactt caactgtttc aacaggtgca acgtatgtat
    11341 gttcttgcgt tgctgtttca gttgtagatg ctgtattttc tgttgttttg ttttctgttg
    11401 ttgctgaaga atcatttgaa gttgggattg ttaattgttc accaacataa atgcggttga
    11461 tgtttgcgat cgaatttttt tcagcaattg catcgatcat tgagttgtct cctgcaaatt
    11521 tttgtgagat cttagataat gaatcacctg attttactgt ataaacttca tcagcgtgag
    11581 catttgtagt tcctacaaac attgcagtag caccagcagc aaaagttgta cctaaaacaa
    11641 ttttcttaaa tgaattcata ttaaactcct ttttttctcc gttttttatt ctttcgacgc
    11701 tattaacttt atcatttaga aatatttcat aggtaaaaga aacatgacat ttaatgacaa
    11761 aataacgaat tatttaatat ttgtaacatt taaacaaata tttttttgat acaaaaccat
    11821 aaaagccttg ttgtataagc aaagtaatcg tttttctatg tttttttatt tttgaatgaa
    11881 aaacgcagat tctgtaacat tcactgcaat aatccataaa aaaaagcaaa aaaatttttg
    11941 atttttatca aattctctta aaaaaatgta aaaaatacaa aaaaaccagt aattgttaca
    12001 acaattactg gacaaaaa
//
LOCUS       DACDYI010000083        11679 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.083, whole genome shotgun sequence.
ACCESSION   DACDYI010000083 DACDYI010000000
VERSION     DACDYI010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.083"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            132..1391
                     /gene="lysA"
                     /locus_tag="IWM52_002437"
     CDS             132..1391
                     /gene="lysA"
                     /locus_tag="IWM52_002437"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAP9974659.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1378..2178
                     /gene="dapF"
                     /locus_tag="IWM52_002438"
     CDS             1378..2178
                     /gene="dapF"
                     /locus_tag="IWM52_002438"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAP9974660.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2181..3062
                     /locus_tag="IWM52_002439"
     CDS             2181..3062
                     /locus_tag="IWM52_002439"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAP9974661.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3059..3775
                     /locus_tag="IWM52_002440"
     CDS             3059..3775
                     /locus_tag="IWM52_002440"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAP9974662.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3781..4476
                     /gene="dapD"
                     /locus_tag="IWM52_002441"
     CDS             3781..4476
                     /gene="dapD"
                     /locus_tag="IWM52_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAP9974663.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4492..5493
                     /locus_tag="IWM52_002442"
     CDS             4492..5493
                     /locus_tag="IWM52_002442"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP9974664.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5490..6851
                     /locus_tag="IWM52_002443"
     CDS             5490..6851
                     /locus_tag="IWM52_002443"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAP9974665.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6811..7962
                     /locus_tag="IWM52_002444"
     CDS             6811..7962
                     /locus_tag="IWM52_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAP9974666.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7937..9106
                     /locus_tag="IWM52_002445"
     CDS             7937..9106
                     /locus_tag="IWM52_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9974667.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9281..10660
                     /locus_tag="IWM52_002446"
     CDS             9281..10660
                     /locus_tag="IWM52_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9974668.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10941..11679
                     /locus_tag="IWM52_002447"
                     /pseudo
     CDS             10941..11679
                     /locus_tag="IWM52_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         3898 a         2040 c         2597 g         3144 t
ORIGIN      
        1 tttaaaaaaa tatttcgaaa tatactagac aattcagaaa attttgttta atattattcc
       61 aataaaattc aaatctttat caaatgaaat tccaataaaa acggaaaaaa agtgaaaaga
      121 ggaatgaaaa aatgacagaa ttgataattg gcggagtcac agcaagcaca ttggcagaaa
      181 agtataagac acctctgtat gtgtatgatg aagaaaagat gagacaaacg atgctgtcat
      241 tcaagcgagg ctttgtatcg aaacgttttg atacaaaagt gctttacgcc tctaaagcat
      301 tccaaacgat tgagatgctg catttagttc gtgaatatgg attcgggcta gatgttgtca
      361 gtggtgggga aatctatacc gctttgcaag ctgattttcc tagagatact atctacttcc
      421 atggtaacaa caagacgcca gaggaactga tctatgcact tgaaaatgat ttgttgcata
      481 ttgttgccga taatttgatg gaagttgaat tattagcaga attaagccaa cgctatttaa
      541 aaaaaatctg tatcatgctt cggttgaatg tcggagtaga agcacatact catgaatata
      601 tcgtcactgc gcatattgat tcaaaattcg gcatgagcta tgaaagtaaa gagtgccaac
      661 aagcccttca gttgataaaa gaaagcagct atctagagtt agaaggattt catgctcata
      721 tcgggtcaca aatttttgat gttactgctt ggttagctga aatcgacaaa ttgacgaatt
      781 acttaaccga ttttgaccaa cctctttcct tgaatcttgg cggagggttt gggattcgtt
      841 atacacaaaa tgatcagccg atcccagtcg aagaggcact aaaaaaactg attgaatata
      901 cggaagaagc tttggaaaaa cgaaaactga ccattcgtca attactgatc gaaccgggta
      961 gaagtattgt tggagaagct ggtacaacac tatacacgat tggtttcatc aaaaagacac
     1021 cacataaaaa atattacttt gtagatggcg ggatgacaga taatatccgt ccagctctgt
     1081 atcaggcaga atacgattgt gatttagcag caaaactgtc ggaagaaaaa acagagaaag
     1141 tgacgattgc aggaaaaatg tgtgaatcag gcgatgtggt aattaaagaa gcttatttgc
     1201 cgaaagcaga atcgggtgat cttttagctg tttattccac gggagcttat ggatactcga
     1261 tgagcagcaa ctacaatcga gcgacacgac cagcagttgt ttttgtcaat aatggccaat
     1321 caagactgat tgttcgcaga caagcatttt ctgatctact aaggggggaa gtttcctatg
     1381 atccttcata aatataatgg ctgcggaaat gattttatct tattagatta cgcagaagac
     1441 acagattata gtcaattagc cgcttcttta tgcacaaaag aaaaatatga tacagacgga
     1501 ttgatcgccg taaaaatcga tccgttggaa atgatctatt acaacaaaga tggcagccgc
     1561 gcgccgatgt gcggaaatgg cattcgctgt tttgctcgat acgtaaatga tcagaacatg
     1621 atgaatgaac aggaattcaa cgtagagaca ttagcaggca caagaaaaat tacggtgttg
     1681 gatccctcac ctttttattg cagcgtagat atgggagtgc cagatgattc tccggaaaaa
     1741 atcaaagcta atcaaactta tccaataaaa aaccaggaaa tagagatcaa tggtgaaaaa
     1801 gtaacgatca ctagtttatt tttaggaacg attcataccg ttgtttttgt tgaaaatgcg
     1861 cgtgaggaag tctggagaaa aagaggtgaa ctgatctgtc atcatccttt gttcaaagaa
     1921 aaaaccaatg tcaactttgt agaagtagtc aatgaacaag aattgatcgt ccgtacctat
     1981 gaaagaggag taggttggac acttgcttgc ggtacaggtt gctgtgcctc ttatgtggtt
     2041 gctaaggaaa ctgggaaaat ttcagcagat caggtagttg ttcacttgga acaaggagac
     2101 ttgatgatct ctggtgagga tgcgatacaa atggctggcc cagcagttca tgaatggaca
     2161 agaatattgg aggaatgaag atgataaaag gttcgatcgt ggcgctgatc acgccgatga
     2221 atgaagaggg atcagtagat tatgctggtt tggaaaaatt gatccaattc catttagatg
     2281 aacaaacaga tggattatta gttttaggta caacaggaga atcttctacg ctcacacaat
     2341 ctgaggagga gcagatcctt caactgacag taaagaaagt ggctggccgt gtgccggtga
     2401 ttgcaggagc tggaacgaat aatacgaaag aaacgattga aaaagcgaag cattttgctt
     2461 ctttgggggc agacgcactt ttggtcatca ccccttacta caacaaaaca agtgatgcag
     2521 gactagctgc tcattttaca gcaattgcag aagcctcacc gattccgctt attttgtata
     2581 atgttccttc tagaacaggc atgtcgattc caatccatgt attagtaaat ttagcagaac
     2641 atcctaatat cattggtttg aaagaagcct caggagatat ggcttatgtg atggatgccg
     2701 ctcggttgat tggagaagaa ttcttccttt acagtggaaa tgatgatttg atcctaccag
     2761 taatgagtgt aggaggaagc ggtgtaataa gtgtatgggc gaatatccaa cctaaaatag
     2821 tccatgaatt agtaaaagat acgcaggacg gaagatggca gcaagcaaaa gaaaagcaat
     2881 taaatgcact tgaactgatc catgccttgt tcagtgagac caatcctatt cctgtcaaag
     2941 cagccatgtc attattagac ttaccttccg gacctctacg cctgccttta gtttctcttt
     3001 caaaagagaa aaagaaacaa ttagctcagc ttttgttaaa agaaaggacg gttgaaaaat
     3061 gaaaatcgga ctgattggca gtggacagat gggaaataga gtcgaagaag ttgcacaaga
     3121 aagaggagat acggtccttc ttttacggac ggcacctaaa gaagcaacta cgcttgcttt
     3181 tacggaactg ccagaactgc ttattgattt ttctcatcca gataatttag agaaaatcct
     3241 ttcgtatagc ttatcatatc aacttccgtt agtcattggc acgacaggtt atacacaatc
     3301 acagttccaa gaaataaaag aacatgccaa acatgtacct gtgatgtata gtgctaattt
     3361 ttcgttcggc atcatggtga tgaatcaact aatcaagcaa gcagctgcaa tgttgcaagg
     3421 ctggcagatc gaactgattg aaaaacatca tagtcgtaaa aaagatgcac catctggtac
     3481 tgcaaatatg ctgcttcaaa caatccaaga agtgcaaaag ctgcagcccg tatacaactg
     3541 gcaagggaaa aaaagagaag aaaaccaaat tggtgtacac agtattcgtg caggttcttt
     3601 acctggtgaa catgatgtat tgtttgctgc tactgatgaa atatttacga tcaaacatga
     3661 atctttttct aatcgaatct ttgctgcagg cgcagtagaa gcagcggact ggttgaaaga
     3721 tcagtcacca ggttattaca aattagaaga tttattaatg gacaaagggg tgtaaaaaac
     3781 gtggaagctc aagaaatcat ccgctacatt caaacggcat ctaaaaagac gcctgtgaaa
     3841 gtctatctga atgtaaccga gccaatcgaa tttaaagaaa gtaaagtttt tggtgaagga
     3901 agcagcttcg ttgtttttgg tgactatgag acgattgctc cggttttaga agctgaatca
     3961 gaaaaaatca ttgaaatcga aatcgaaact gctgcaagat acagcgcagt tcctttgcta
     4021 gatattaaaa aaattaatgc tcgtatcgaa ccaggtgcta ttatcagaga tcaagtgtca
     4081 atcggcaaca atgccgtgat catgatggga gcgattatca atattggtgc agtaatcggt
     4141 gaaaacacga tgattgatat gggtgctgtc ttaggtggac gagcgacagt gggcaaaaac
     4201 tgccatattg gtgcaggagc tgtcttagca ggtgtgatcg aaccagcatc ggccaaaccg
     4261 gttatcgtag aagacggcgt actggtaggt gcgaatgcag tgattgttga aggtgtccat
     4321 atcggaaaag atgcagtcgt ggcagcagga gcagtggttt tagaagatgt ggcagcagaa
     4381 actgttgtcg gtggaatacc tgcacgtgta ttaaaaataa gtgatgatca gacaaaagaa
     4441 aatacagcat tgattgctgc gttacgcgaa ttatagagga gatggagaaa aatgcgcgaa
     4501 atagatacct gtattgtggg ggcaaccgga ttagtcggcc aaacgatgct caaagtgtta
     4561 gaagaatatg attttccagt aggacgattg aaattattag cttcaaaacg ttcagccggt
     4621 aaagtttgtg tattcaagaa aaagaaacac actgtacagg aattgacaga aacttcattt
     4681 gaaggatatg atttagcgct attttctgca ggtgcttctg tatccaaaga gtttgcgcca
     4741 atcgcacgaa gtcagggact atttgtcata gataattcct cagcatggcg ggaagattcg
     4801 gatattccat taatcgttcc agaggtcaat ctggaagact atacattgaa tcggctgatt
     4861 gcaaatccaa attgttcaac cattcaagcg gtacttccgc ttaaagcttt acaagaagcc
     4921 tttggtatcc agcgagtcaa ttattcgacc tatcaagcag tttctggttc cgggcaaaaa
     4981 ggcattgaag atcttcatcg tacgcaaaat gacgaggaac cagttttgta tccttataat
     5041 attagccaaa cggtgattcc ggaaatcgat cagcctttgg aaaatgggta tacaaaagaa
     5101 gaacagaaaa tgataaatga gacccgtaag attctgcatc agcctgcttt gccagtatca
     5161 gcaacctgcg tacgtgtgcc aatcgaaaat ggacatggag tctctgtggc agttcaactg
     5221 caaaaagagt ttacgacgga agaaattaga gagtgtctca gccagtttcc agggattcaa
     5281 ttagaagatg atctagcagc acataaatat ccgacgagca ttcttgctag aggaacagat
     5341 atggtttatg ttgggaggat cagaaaagac acttctttag agaacggatt gttgttctat
     5401 acgactgccg ataacatacg caaaggagcc gctgcgaatg ccgtacagat tgctgcagct
     5461 ttaatgcaga aaatagcgga ggtggcaaga tgagtaatac gctgaaagta gcgaaattcg
     5521 gcggaacatc gatgtctcat gaagagcaat tcaaaaaagt ccgtacaatt gttctttcag
     5581 atcccaatcg ccaagtgatc gttgtttctg ctttaggcaa acgtacgtca gatgatgaca
     5641 aagtaacaga cttactttat ttgatttatg cttaccttca gcatcatgtt gattggacac
     5701 ccctttggaa aaggatttgt gatcgtttca ttcaagtaag agatgttctt gccctgcagt
     5761 tacctattga agatgaacta gcacgcatac agaaacaact ggaagacgaa aagataactg
     5821 aagattattt agtaagtaga ggtgaatatc tgactgctca actgatggct gagtatcttg
     5881 gttaccagtt tattgacgca aaagacctga ttttctttga ctatgatggg aaaatcgatc
     5941 aggaaaaaac agcacaggaa atcacaaaag catttaaaag atatccgaaa atggttgttc
     6001 cagggtttta cggggcgaac cctgcaggaa aagaaaaatt attgggccgt gggggaagcg
     6061 acattaccgg cgcgatactg gcgaagatgt tgcaagcgga aagttatgag aattggacgg
     6121 atgtttcagg aatcatgatg gctgatccgc gaatcatcga tcatcctaaa atcatcgagg
     6181 aaatctcttt tagagaactg agagagatgg cttatatggg ggctaacgtt cttcatgaag
     6241 cagctgtttt cccagttcaa gaggctgata tccccatcca aataaaaaat accaatgatc
     6301 ctttcgctca agggacaaaa atatcgaatc acgaaataga aaaggaaaat ggactgacag
     6361 gaatagctgg acgcaaggat tttctttcca tcactttatt caaacgtcat atgtccgatg
     6421 aaatcgggtt tatctggaaa gcaatgagca tttttgctaa acatgagata tcaatcgaac
     6481 atattccttc agggatcgac aatatcggag tgatcgtctc agcagaagcg attgccgata
     6541 agttatttct catcacgaaa gaattaaaag aaacacttgg cgtggaagaa atcgtcgtga
     6601 tcgaagactt agctttgatc tctgtcgttg ggggtccgca taaagaattg attggattat
     6661 ctgggaaagt gttatcgata ttgaacaaat tagaaattcg cacatcgatc ttatcacaag
     6721 gagcacagga gttgaatctg atcatcggtg taccgaataa tcaatatgaa acagtagtga
     6781 agggtatcta tgaagggatg gtgaatacgg atgctgaaag aatatcgaat ggcactccat
     6841 caaattcctg aaatcgggtt tcatgaattc aagacaaaaa aatatttata tgaacaagta
     6901 aaaaacagcg gggccgttat ccatgaaatc ggcgaaacag gactactcct ttattttgat
     6961 aatcagcaag aagaaacgat tgcttttcga acagatatcg atgcactgcc agtcaaagaa
     7021 gaaacgaatc tctcttttgc ttctaagcat gaaggttata tgcatgcatg tggtcatgac
     7081 ggacatatgg cgatgctttt agggttgact gactttataa ctaaacacat tgaggatctt
     7141 atttataatg tagtactgat ctatcagcca tcagaagaaa tttccggtgg cgcacaaagc
     7201 gttattcagt caggattgtt ggataaatat gaagtacaag caatatttgg ctttcatcta
     7261 tggcctggac tagctaaggg agaagttttc tctcgaccag gacctttgat ggcacaaagc
     7321 agtgaaacag acattatcat taaaggccag tctgcacata ttgcttctag tgaaaaagga
     7381 atcgatagtc tggaggcagc tgtacgattt ttgaagaatg tttatgattt tgaccggcaa
     7441 ttaccggcag agtggaatca tctaatgaaa tttggccaaa tccaaggtgg aacgatccga
     7501 aatgttttag ctggagaagt tattgtatcc ggaagtatcc gttcttacag ttcagatatg
     7561 caaaattatt tgaagagggg attagaccat ttagcagaac agtttcactc tacagctact
     7621 atttcttttc gctataatga cggctatcca gctgtaatca accatagagg cttatatgca
     7681 gcactgttag attcaaacaa actacatgaa ttagatgctc cagttcttca agccgaagat
     7741 tttggcatat atacgcaaca atatccatgt gtatttttct ttttaggtgt cggtgatacc
     7801 cctgctttac atgatccgac gttcgatttt gatatggatg ttttagaaaa tggtgtagaa
     7861 tggtacaaaa cgattctttt tacagaaaat cttcttgtaa aaatcgatag cagttcaaaa
     7921 gaggagcgag taaataatga tgaatttatc caatcgattt agtcctaggc tttctagaat
     7981 agaagtatca aaaatacgac aattcgatca gcaaatatcg tcgataccag atgtgatcaa
     8041 actcaccctg ggagaaccgg attttccaac tcctgaacat gtcaagcaag caggaatagc
     8101 agcgattgaa gaagactttt ctcactatac cggaatgaga ggattagagg aactgcggga
     8161 agcggcttgt atatttcagc aacagcgtta tggattaact tatgatccgc aaacggaagt
     8221 tttgacgaca gttggtgcaa cggaagcgat tgcctctgcg ttgttatctg ttttggaaga
     8281 aggagacaag gttcttattc cagcgcctgc ttattcagga tatcagccat tagtggaact
     8341 agctggtgct gaattgatcc cgatagatac ttctgacaca gggtttgtct gtcagccgga
     8401 acaatttgaa cgagcatttg aacaatatgg atcagctgtc aaagcagtca ttttaaacta
     8461 tcctaacaat cctactggga cgacattgtc tgcaaaacaa ttgaaagcaa tcgctgaggt
     8521 tttaaaaaaa tatccggtat ttgttataag tgatgaagta tatgctgaat tgacttatag
     8581 tggaacgcat atgtccatag ctacttatct tccagaacaa acaatcgtga taagcgggtt
     8641 atccaagtca catgcgatga ctggttggcg agtgggcttt atttttgctc aaaaagccct
     8701 tattgacgaa ctaattaaag tccatcaata tctggtgact gccgctacaa cgatgtcgca
     8761 aaaagctgcg gctgaagcgc taataaatgg ggtgaaagat agcgagaata tgaaagagca
     8821 atatcaaaca cgaagagact atctggtcca gcaattggcg cctttaggat ttgaagtgac
     8881 ccagccaaac ggtgcatttt acttgttttg caaactccca gctgccattc aaactgactc
     8941 ttggcagttt tgtgtagaat tagcggaaca ggcaaaagtt gcgtgtatcc caggaattgc
     9001 atttggccca gaaggagaag gttatatccg gatttcttat gctagcagca tggaaaaatt
     9061 acatgaggcc tgcaaacgga tcaaagactt tttaacagaa cagtgattca tagattttat
     9121 caaaaaaaca atcgttaatt ttgcggtata ttctgcaagg agacgattgt tttttaaatt
     9181 tagttcaaat gaacgcgttt cttttgattc ataactaatg taatgttaag tttaaattgt
     9241 ttagaatgat tttaaaagaa tattcaagga ggtttcatca atggaaaaaa tcaaagttgt
     9301 cattgtaggt gcatctcatg gagggcatca atcgatcttg gaactattga cacgatacgg
     9361 tgaaaatgta gatatcacgc tatttgaagc aggagactat atttccttca tgtcatgtgg
     9421 tatggagctg tatttagaag acaaggtaac agatgtaaat gatgtacgta atttcagacc
     9481 agaaaacttt ccgcaatcca atgttcatat tttaaataat cacgaagtaa aaacgatcga
     9541 tgccgataaa aaaacagtaa cggttactag cgcatcagat aacgaaacaa aagaatatcc
     9601 ttatgacaaa ttgattttga gttctggtgt caaaccaaac aatctgccag taccaggagc
     9661 agatctagaa aatgtctact tgatgcgtgg atacaattgg gcaaccaaaa tcaaagaaaa
     9721 attaaatgat ccggctatta aaaaaatcgc tgtcgtaggt gcgggttaca ttgggatcga
     9781 agcagcagaa gcaagccgaa aagctgggaa agaagtcgta ttgcttgatg tgatcgatag
     9841 acctttaggc acttatttag atgctgagat gacagatatt ttagaacaac acctaaatga
     9901 aaacggtatt gaagttttga caaatgcaaa aatcgaagct tttactggta acggaaaagt
     9961 tgaggcaatc aaaacaagtg aaaaagaaat cccagcagat ctagtcattc aagctgccgg
    10021 tgtgaaggca aatacagaat ggttaaaagg gatcgtcgac ttagatgaac gtggctggat
    10081 ccaaacaaat gaatacttgc aaacgaatct tccagatgtg tatgcagtag gagatgctac
    10141 acttgcttat tctatcccag cacgcaaaaa aatgccgatc gctttagcga ctgttgcacg
    10201 tagagaagca cgttatgtag taaaacatct ttttgaagaa gtaccaaaca aaccgttcgg
    10261 cggagtcgta ggttcctctg cattaagtgt ctttgactat cattttgcag caagcggatt
    10321 gaatagcttt acagcagcaa aagcaggagt agatgtacga gtgtcttatt atgaagatac
    10381 gttacgacca aaatatgtac cagaagaaca aggaaatcca aaagtgtcca tccaattatt
    10441 ctcggatcca atgacccatc aattgctagg aggagcagta ttatctacct atgatgttac
    10501 aggtcaagga aatgtattgg cactagcgat ccaaaacaaa ctgactttag aagaactagc
    10561 agaagctgac ttcttcttcc agccaggctt tgaccgacaa tggagcatgt tgaatctagc
    10621 cgctcaacaa gcgttaggag aagaaccatt tgtagaataa gaaaacgaaa tatttgaaaa
    10681 gaggttgtga aaaagctgcc ttgcatcaag taataagaac agccaagtaa aaacagcttc
    10741 ttatgttttt aacttggctg gacttattac cgcagatgca agcttttgaa caccgtttat
    10801 tttcaaaaaa gggactgtga cacgatttgt gtaccgaccc ccaaaagtta gaccaaaaaa
    10861 tctaactttt ggaggtcggt attttttatg gttaaatata cttttgaact taaattaaag
    10921 atcgttcatg attatttgga tggcaaaggc gattggaccg taaaaatcca gatcaagaga
    10981 ttgaagcgga aatgcttaaa attcgggaga agcacaagga ttatggctgt ttacggatga
    11041 ccaatgaact aagaaaccgc ggtttctcta tcaataaaaa gaaagttcag cgcttgatca
    11101 agaagctagg aataaaagta acggcataca cgcgtaaatc acgtcgctac aattcttatc
    11161 gaggaaaagt tggagcagta gccaaaaatc ggatccatcg tcgtttctac accagtatct
    11221 gccatcagaa aatcacgacg gatacaactg aatttaaata ccatgaagtg gatgccaaag
    11281 gcattatccg tcaaaagaaa ttgtatctag atccttttat ggatatgttc aatagtgaga
    11341 ttttatccta tcgtatctct gaaaagccca atgcattagc ggtaatggaa ggtttggagg
    11401 aagctatcca gaccactaac gattgtccct atcgtcgtac gtttcactca gatcaaggtt
    11461 gggcgtatca aatgaaagct tatagaaaca aattgaagga gtacaaaatc tttcaaagca
    11521 tgtctcggaa agggaactgc ttagataatt cacttatgga gaattttttt ggcttattaa
    11581 aacaagagat ttttcacggg aaagtttata actgtttcga ggaattaaaa tcagccatcg
    11641 atagctatat ctattactac aataatgaac ggataaaac
//
LOCUS       DACDYI010000084        11522 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.084, whole genome shotgun sequence.
ACCESSION   DACDYI010000084 DACDYI010000000
VERSION     DACDYI010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.084"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(102..632)
                     /locus_tag="IWM52_002448"
                     /pseudo
     CDS             complement(102..632)
                     /locus_tag="IWM52_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
     gene            704..1996
                     /locus_tag="IWM52_002449"
     CDS             704..1996
                     /locus_tag="IWM52_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9974669.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSECRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
     gene            complement(2208..3050)
                     /locus_tag="IWM52_002450"
     CDS             complement(2208..3050)
                     /locus_tag="IWM52_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9974670.1"
                     /translation="MISIKKVLNSSVVLVEENGKEMIALGKGIGFGQKAGDEISGDKV
                     DKIFLPFEEKKSSQLLELVEEIPIEFFEITRTIVFEAEKGLDKKLNPGVYLTLTDHLH
                     FAVERAQKGVNIANRLYWEIKIYYPKEFQIGKYALTLLNEQYGIDLPKEEASNIAFHL
                     INAQSEDPDSGAGFKYAKMIGGIVNLVRYSLQKDIDTNSIHYARFITHVRFFVERYYS
                     DALLDDGEEEFYNQMWILYPSAMESAAKIKDYIEKVYHKTIPNDEVVYLGVHINRLMK
                     HANG"
     gene            complement(3087..4553)
                     /locus_tag="IWM52_002451"
     CDS             complement(3087..4553)
                     /locus_tag="IWM52_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9974671.1"
                     /translation="MTNTTSFPKGFLWGGAIAANQVEGAWNVDGKGLSVADMATYKPN
                     LDNKDFAAHMHVTLQSIKEASEDKSDKWYPKRRGVDFYHHYKEDLALFAEMGFKTLRL
                     SIAWTRLFPTGEELEPNEKGVAFYENVFKEMQRLGIEPIVTLSHYEMPLALAVKYNGW
                     VERRVIDDFVRFANVCFDRFGKYVKYWLTFNEIDSIHRHPFTTAGIIQEKSAEGQEAQ
                     DIYQALHHQFVASALVTKDAHEKIPGSQVGCMLTKLMTYPLTCAPEDVELTLKKNLEN
                     NFYADVQVKGEYPALIEKDLENRGIQIKMEDEDREILKKHTVDFVSFSYYMSMAESAD
                     PNAERTPGNTVLGVKNPHLPSTDWGWQIDPKGLKISLIELYDRYQVPLMIVENGMGAV
                     DQVVDGKIHDPYRIDYFKAHFQQMKEAIDEGVDLIAYTSWGPIDLVSAGTSQMSKRYG
                     FIYVDADDEGNGTYNRLKKDSFNWYKEVIETNGASLFE"
     gene            complement(4609..6453)
                     /locus_tag="IWM52_002452"
     CDS             complement(4609..6453)
                     /locus_tag="IWM52_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9974672.1"
                     /translation="MNYKQTAEEVLQYIGGEENISHLEHCSTRLRFTLKDNSKVNMSQ
                     LEKVDGVMGVRQNVQCQVIIGNDVVEVYDEVKALLGDNVSNTEEAPKQKQKLGSVLLD
                     FIISIFQPLIPAIAGGGVLKSLLLLASILGLISDQSQTYQILNLVGGAPLYFLPILVA
                     MTTANKLKVNQLVAVSAVGALILPEMTAMLAEGATFLSFGLDNIAYASQVFPAILTVL
                     FYAQLEKWFTKVSPKPIRIFFVPMMSLLITVPIALLLLGPLGYNVGTAFSSIIVSLYN
                     QFGWVATGVLAAVLPLMVVTGMHKAMIPYAVSSMSELGAELLYLPASLAHNISEAGAC
                     FAVSIKTKDKKLKSTAVSAGISALFGITEPALYGVTILHKRVLYAVMFSSLIGGAFAG
                     IVALQAFALVGPGIASITMFADKADPMNLVWAFAVSGISFVVAFAAALVLYKDKEVVN
                     EVETKMETAVVSDAVLSSPVNGKVISLSEVKDTVFSSGMVGDGVAIIPEEGILVAPED
                     GEITMVFETKHAIGLKTANGAELLFHIGIDTVQLEGKGFEAFVRSGEKVAKGQKLIAF
                     DIDLIKEAGFDPTVICVVTNKDNYTIRSFNLEKLVTNDSDLLAISPIA"
     gene            7055..8005
                     /locus_tag="IWM52_002453"
     CDS             7055..8005
                     /locus_tag="IWM52_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9974673.1"
                     /translation="MKNDIVTYANTKCEEIGISNLEECFFDTNDLSQFLRTGRTNASK
                     LANDLVAKNVLFKINTRPVLFAIKERFEEHFLIPVKDVYESISELKTDLEPNSTDSIL
                     QKMIGYDMSLSDAVEQMKTAVFYPGRGLPMMITGDTGVGKSFFVHTAYQFMKKVNVIH
                     ENAPFKVLNCAQYHNNPELLSGILFGYVKGAFTGADEDRNGLLQDADGGVLFLDEVHR
                     LPPEGQEKLFVYMDGGTYSRVGETIHHASNVRLVFATTEGKEDFLETFMRRIPIHIHI
                     PNIEERGILEKKQIIENTLFSESKNFSKAIDVSPKVTSVA"
     gene            complement(8050..8683)
                     /locus_tag="IWM52_002454"
                     /pseudo
     CDS             complement(8050..8683)
                     /locus_tag="IWM52_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            complement(8943..9491)
                     /locus_tag="IWM52_002455"
     CDS             complement(8943..9491)
                     /locus_tag="IWM52_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974674.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(9492..10349)
                     /locus_tag="IWM52_002456"
     CDS             complement(9492..10349)
                     /locus_tag="IWM52_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9974675.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(10635..10922)
                     /locus_tag="IWM52_002457"
     CDS             complement(10635..10922)
                     /locus_tag="IWM52_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP9974676.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            complement(10912..11241)
                     /locus_tag="IWM52_002458"
     CDS             complement(10912..11241)
                     /locus_tag="IWM52_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9974677.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(11318..11464)
                     /locus_tag="IWM52_002459"
                     /pseudo
     CDS             complement(11318..11464)
                     /locus_tag="IWM52_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
BASE COUNT         3506 a         2318 c         1810 g         3888 t
ORIGIN      
        1 catttccatt tcatttacct ctttttcgtt taattatact aaagttctat ccaagcgaca
       61 agctgtgtat aataaaaata tacccgtggt gctagttaat tttaataccc taaagaatat
      121 ctcaaagtcc cttcaaaatg ttgctgtgct cggcttaaaa gaatactgta cgataataat
      181 atgctttcaa acttactttc tagcccttga atagaacgtg atttgaaatt ttgaaaacct
      241 agtctttcga gagaagaaaa aacagtttct actgcttttc tttgtctagt aagtaatcga
      301 tggtctatct tccctagttt atccatgttt tttcgtgacg gaactaagac tgtaatgccc
      361 ttaagggcaa gcttttcatg gattttatgg ctgacatagc ctttatctcc aaggatattt
      421 ggaaggtttg cttcttccga taaagtctct aaaactttca catcatgaac actaggatcc
      481 gtgattgaat aagtaactgg aaagcctgtt ttagtgacga taatatgaat tttaagacca
      541 taaaaataag cgtttttcgt tgagttatag cccctacagc tctatacttt ttgatgttgg
      601 tggatgacac cagcatcttt tttattttcc aaaacatata gaaaagacac cttgaaatcc
      661 tttagaatta atttggcgaa ccaacccaaa aggaggaatc aagatgtctt acactcacct
      721 tatcaaagaa acattagata ttctagactt atcagttact tttaacgaaa attgtctcac
      781 aaaagaaaaa tacaaagggc aaatctgtca tatttatcgt ggaaacttga tttatacagc
      841 ccaagaatgc atccactgta aacaccagat tgcttcagat attgtgcgtt ggggtacaac
      901 aaccgtccgg ttgttaatga acgatgtttc tgaatgccgt acctacctcg aattgaaaaa
      961 gcaacgcttc aaatgcaaag cttgtcagcg gacctttgtc gcagatactt ctgttgcgaa
     1021 aaaacactgt ttcatcagtg agaaagtccg ctggtctgtc gtgactcgtc tgaaaaaaaa
     1081 tacctcaatg acagagatcg ccgctcagaa aaatctatcg gtctcttctg tttaccgtat
     1141 tatgaagcga ttttatcgtc cgttgaatcc ttttcggaca ccgttaccta aggtactttg
     1201 ttttgatgaa ttcaaatcag ttcggggtgt ttcaggtgct atgagtttca tcatgatgga
     1261 tggacaaaca cagcgcttgt tggacattgt cgagaaccga caactgcctt ttttaaaacg
     1321 ttattttagt catttttcac gagaaattcg tgaagcggtc gaatggatcg tgattgatat
     1381 gtacgctcct tatgtatcgc tcgttaaaaa gctgtttcca aaagcacagc tgattattga
     1441 ccggtttcac atcgtccaac atattggtcg tacctttcgc aatcatcgaa tcaaagaaac
     1501 gaaccaacta ttaaaaagca aagaacaaaa acactatcaa ttaggtaagc aattaaaaag
     1561 atactggaaa cttcttcaaa aagatgaacg caagcttgat tatactcgac gcctttggcg
     1621 acctggcttc aaagctcatt taacagagac cgatattgtg gatcgcttac tgaaagggag
     1681 ccctgctttg cgtgtaggct atcagttata tcaagacttt ctttacgctg taaaggaacg
     1741 agactacgta tcgtttgaag aactacttac caataatatc atgcttcccg aaggctatca
     1801 gacgaccttg agaacgttcc agaaatttct gccacaaatc aaaaatgctt tacaacaaag
     1861 ttattcaaat ggcccacttg aatgtctgaa caaccatatc aaagtgttaa aacgaaatgc
     1921 atatggcttt cgtagctttt ataatttcaa attgcgcatc atgattcgtc atggcaacgc
     1981 attgattttc aactaaaaaa gatcaagagg aactgatcca cttgatctcc aattattcta
     2041 agatttctct ttaccaacat catttgacaa agagccgaga ttttttatta tcttacgtta
     2101 atccttttta aatgtctctt atagataaca aacagctgga aatcaccttg tttttactat
     2161 aatccatctc taaagatttt aaaagcatca atagtattac tgagcgctca cccattagca
     2221 tgtttcatca accgattgat gtggacacct aggtatacaa cttcatcgtt cggaattgtt
     2281 ttatgataaa ctttttcaat gtagtctttg atcttagctg cgctttccat ggcagacggg
     2341 tataagatcc acatttgatt gtagaattct tcctcaccat catccaacaa agcatcagaa
     2401 taatatcttt ctacaaagaa acgtacatgt gtaatgaagc gggcataatg gatcgaatta
     2461 gtatcgatat ctttctgcaa cgaataccgg accaaattga cgatcccgcc gatcattttt
     2521 gcatacttga atccagcgcc gctatcagga tcttccgatt gtgcattgat caaatggaaa
     2581 gcaatgtttg aggcttcctc tttaggaaga tcaattccat attgctcgtt caaaagggtg
     2641 agtgcgtact tcccaatttg aaattctttg ggatagtaga tttttatttc ccagtacaaa
     2701 cggtttgcaa tatttacccc tttttgtgcg cgttcaactg caaaatgcag gtggtctgtt
     2761 aaggttaaat agactccagg attcagtttt ttatctaatc ctttttctgc ttcaaaaacg
     2821 atagtacgag tgatttcaaa aaattcaata ggtatttctt caactaactc taatagttgt
     2881 gaagatttct tttcttcaaa aggcagaaat attttatcta ctttatctcc tgatatttca
     2941 tccccagctt tctgtccaaa gcctatccct ttacctaatg caatcatttc tttgccattt
     3001 tcttcaacaa gtaccacact tgaattcaat acttttttta ttgaaatcac tcattatccc
     3061 cctatcgctg cttaaaaaga tcgagactac tcgaataagg atgcaccgtt tgtttcgatc
     3121 acttctttgt accagttgaa cgaatccttt ttcaagcggt tatatgtccc atttccttca
     3181 tcgtctgcat ccacataaat aaatccatat cgtttagaca tttgagaagt tcctgcacta
     3241 actaaatcaa ttggtcccca gctagtataa gctatcaaat ctacaccttc atcgattgct
     3301 tctttcattt gttggaagtg tgctttaaag tagtcgatgc ggtacggatc atggatttta
     3361 ccatctacta cctgatcgac tgctcccata ccattttcca caatcatcaa cggtacttgg
     3421 taacgatcat aaagttcgat caacgaaatt ttcaaacctt ttggatcgat ttgccagccc
     3481 caatctgttg aaggcaaatg aggattctta acccctaaaa cagtatttcc cggtgtgcgt
     3541 tctgcgtttg gatcagctga ttctgccatg gacatgtaat agctgaaaga aacaaaatct
     3601 acggtatgtt tctttaaaat ttctcgatct tcatcttcca ttttaatctg aattccccga
     3661 ttttccaaat ccttttcgat tagagcagga tattctccct taacttgaac atctgcataa
     3721 aaattatttt ctaagttctt tttcaaggtc agctcaacgt cttctggtgc gcaagttaat
     3781 ggataagtca tcaatttcgt taacatacaa ccaacttggc ttcctggaat tttttcatga
     3841 gcatcttttg tcactaacgc actcgcaaca aattgatgat gcaatgcttg atagatatct
     3901 tgagcttctt gaccttctgc gcttttctct tggataattc cagctgttgt aaatggatga
     3961 cggtggatgc tgtctatctc attaaatgtc agccaatatt ttacgtattt gccgaagcga
     4021 tcaaaacaaa cgttagcgaa gcgaacaaaa tcatcaatta ctcgacgctc cacccagcca
     4081 ttatatttaa ctgctaaagc taatggcatt tcataatgag atagtgtcac aatcggctca
     4141 attcctaaac gttgcatttc tttaaagacg ttttcataaa atgcaacccc tttttcattc
     4201 ggttccagct cttctcctgt agggaacaag cgagtccaag caatagagag gcgcaaggtt
     4261 ttaaagccca tttcggcaaa taaagctaaa tcttctttat agtgatgata aaaatcaacg
     4321 ccccttcttt ttgggtacca tttatcagat ttgtcttcac tagcttcttt tatagactgc
     4381 agggtgacat gcatatgagc agcaaaatct ttgttgtcta aatttggttt ataagtagcc
     4441 atatccgcta ctgaaagacc tttaccgtct acattccaag caccttctac ttgatttgct
     4501 gctatcgcgc cgccccataa aaatcctttt ggaaaacttg ttgtgtttgt cattattcta
     4561 ttctcctcta ttccttcatt caatatattt tttttcacta tcttgtgttt aagctatcgg
     4621 actgatagct aagagatcac tatcattggt taccaatttt tctaggttaa atgaacggat
     4681 agtgtaatta tccttattgg ttaccacaca gataactgtg gggtcgaatc ctgcttcttt
     4741 gattaaatca atatcaaatg caatcagctt ttgtcctttg gctacttttt caccagaacg
     4801 aacaaatgcc tcaaaccctt ttccttctaa ctgaacagta tcaataccaa tatgaaaaag
     4861 aagttctgca ccattagccg tttttaaacc aatagcatgc ttcgtttcaa ataccattgt
     4921 gatttctcca tcttcaggag ctactaatat cccttcttca ggaatgattg ctactccatc
     4981 acctaccata ccactagaaa aaacagtgtc ttttacttct gatagtgaga ttacttttcc
     5041 attcacggga cttgaaagaa cagcatctga aacaacggcc gtttccatct tagtttcaac
     5101 ttcattgaca acttctttat ctttataaag aaccaacgct gcagcaaaag caaccacaaa
     5161 tgaaatcccc gatactgcaa atgcccaaac cagattcatt gggtcagctt tgtcagcgaa
     5221 catcgtgata cttgcaatcc caggacccac taaagcaaag gcttgaagtg caacgattcc
     5281 tgcaaatgct cccccgatca ggctagaaaa cataacggca tataaaacac gtttgtgcag
     5341 aatcgttacc ccgtaaagag ctggttcagt aattccgaat aatgcagata tacccgcaga
     5401 aacagccgta gatttcaatt ttttgtcttt tgttttgata ctgacagcaa agcatgcgcc
     5461 agcttctgaa atattgtgtg ccaatgaagc cgggagataa agaagttctg cacccaattc
     5521 actcatggat gaaacagcgt aagggatcat tgctttatgc atacccgtta caaccattaa
     5581 tggcagtaca gctgctaaga ctccggttgc aacccatcca aattgattgt acaaagacac
     5641 aatgatagaa gagaaagctg ttcctacatt ataacctaat ggtcctagaa gcaacaaagc
     5701 aataggaact gtaatcaata agctcatcat aggtacaaag aaaatacgaa ttggttttgg
     5761 tgaaactttt gtaaaccatt tttcaagttg agcataaaat aaaacagtta agatggctgg
     5821 gaatacttga gatgcatacg ctatattatc caatccaaaa gataagaatg tagcaccttc
     5881 cgccaacata gctgtcattt caggaaggat caatgcaccg acagcagata ctgcaaccaa
     5941 ttgattgact ttcaatttat tagccgtagt cattgccacc agaataggca agaaataaag
     6001 cggtgccccg ccgaccaaat ttaaaatctg ataggtttga gattggtcac taatcagccc
     6061 taagatagaa gctagtagca acaaggactt caatactcct ccacctgcaa tcgcaggaat
     6121 taaaggttgg aaaatagaaa taataaaatc taatagaact gaacctaatt tttgtttttg
     6181 ttttggtgct tcttcagtat ttgaaacatt gtcacctaat aacgctttca cttcatcata
     6241 cacttcaaca acatcgtttc caatgattac ttggcattgc acattttggc gaacgcccat
     6301 aacgccatcc actttttcta actggctcat atttaccttg ctgttgtctt ttaacgtaaa
     6361 acgtaaacga gttgagcaat gctccaaatg ggatatgttt tcttccccac cgatgtactg
     6421 caatacttct tctgctgttt gtttgtaatt catggtgttt ccctcttttc ttttttgagt
     6481 gatgaacttt tgagtcgaag tggccatcct caacacaaaa aacaggcaga aactaaataa
     6541 attgacaaca aggcaaacgt gtattgaaac gcatacattg ttattcaact tatttaggtc
     6601 ctgcctgcat taccagtcac atgcctgttt aagttatatt caatttaaca tattagaatg
     6661 taaacgtaaa taataaaatg attttttaat cattttttca ttttattcgt tcaatatagt
     6721 tttcgtgttc taaaagtttt gtcttttttc taagaatatc ttccaacata tataactctt
     6781 attctcttct atcttctagc agatgttctg tctttttaag acctagtata aaaagaatgg
     6841 gttcattttt tctttcttgc aggatgaggt tttttatata aactttattt gatataaaga
     6901 aaaggcattc ataattgtgc tatactacta aaaaatgata atacagtgaa ttagattagc
     6961 gctttattaa attaagagga cagcatatcg atattgcttc cttttttttg ctatttattt
     7021 aagggataga agtttagaaa ggactgttta taagatgaaa aatgatatag tgacttatgc
     7081 aaacacaaaa tgtgaagaaa ttggcatcag taatttagaa gagtgctttt ttgacacaaa
     7141 tgacctgtcg caatttttaa gaacgggaag aacaaatgcc agtaaacttg ccaatgactt
     7201 agttgcaaag aacgttctat ttaaaatcaa tactcgtcca gtgttatttg ccatcaaaga
     7261 gcgctttgaa gagcactttt tgattccggt aaaagatgta tacgaaagta tatctgaatt
     7321 aaaaacagac ttggagccca attctacaga ttccattcta caaaagatga ttgggtatga
     7381 tatgagctta tccgatgcgg tggaacaaat gaaaactgct gtcttctatc ctggaagagg
     7441 gcttcctatg atgatcactg gagatactgg agttggaaaa agtttttttg ttcatactgc
     7501 ttatcagttt atgaaaaaag tgaacgttat tcatgagaat gctcctttta aagtacttaa
     7561 ttgtgcgcaa taccataata atcctgaatt attgtcgggg atactatttg gctatgttaa
     7621 aggtgctttt accggcgcag atgaagatcg aaatggattg ttgcaagatg cagacggtgg
     7681 agtactgttt ttagacgaag tacacagatt gcctcctgaa gggcaagaga aactatttgt
     7741 ttatatggat ggcggaactt attctagagt cggtgaaact atccatcacg cctccaatgt
     7801 acgtttagtt tttgcgacta cagaaggcaa agaggatttt ctggaaacct ttatgcgaag
     7861 aatccctata catatccata tcccaaatat tgaggaacga ggtattttag aaaaaaaaca
     7921 aattatcgaa aatactctct ttagcgaaag caaaaacttt agcaaggcta ttgatgtttc
     7981 tcctaaagtc actagtgttg cataaatata gtcagaatat tctgaattat tattttcttg
     8041 aaaaaagtaa aaaaaatcgt atcccaaact ttcatctaca tttccagaaa gatcttcatg
     8101 ttgataatca attcctgaat caatgaccgc taccttcaca tccttagaac ccttggtttc
     8161 ttcccaagcg gatggcatgc taatctctct taaattagct tgtttactcc aattaggatc
     8221 gttatcgatt tcctaaagaa tcagttgtat ttggctcctc aacatcattc aacggctcta
     8281 gttcaaccac atagtttggc tctgcataat tgaccatatc agaagcatta aatgttctta
     8341 ctgcctcttc cacactaatt tcttcgttca atttcactaa taatacttga tctcgcttat
     8401 tataatcgct ttcatttgtc ctaagagtca aatctttttc caccataact tttacaccca
     8461 ataaattttg cggtttattt ttctctgttg aaattctttg tgtagccttt aaactgacaa
     8521 ttagctcatt gggtacatac tctatttgag agtcatctaa ttggctcata atcctttctt
     8581 tttggttagt atctgcagtt acttgtttag tatgagccat gaacccaaac atactcaccc
     8641 caacaaccaa tcctaaatat attttctttt taaacttttt catctacatc tcctcctaat
     8701 agttttattt tagagttagc tctattactt atttactaac tcaacaacag attatcatac
     8761 acttattaat gaaatcacaa tatctcatta aaacacaccc taaaatcata accgttccta
     8821 tgctcatttt ttggattttt taatcacgta accagaaaaa taaatcccct ctatttcacg
     8881 caaatcaaag aagaactaag taactaccgt ttaaaggtag ttacttagtt cttctttgat
     8941 tattatctaa ctttattatt cattccccac cctttgcgca gtgagtcttc ggtatcttgt
     9001 tttatctgac tggtagttct tattatttct ccacgatcat ctaattctgg aacaattgga
     9061 tatttttctc tgaacaagtc tagttctttc accacgtcat taaacaaatc ttgtttatct
     9121 tcttgcgtaa gattctgttt aatatactca tctaaagctt ttctaagcaa ttcatctctt
     9181 gtcatattag tctcatactt ttctactaaa actctggaag tcgcatttaa caaagtcgac
     9241 agttttaaag gatgcttggt ggaataaacc ttatcaaact ttctgattgg tgcgccaact
     9301 ttttttgttc ttcctctact ctgtctttca acattggagg aagtttttat ttcttgaact
     9361 tggtgcactt ctaattgctt attttcagat ggtgtaattg cttgctgctt tttctttgct
     9421 tctgcttctt tttttatttt ggcgagtcct ttgttgttcc caaaaatatc aagtcctttg
     9481 atgtcagcca attactccac ctccgcatac caattctcgt gttcttccaa ttctttcaaa
     9541 atactgataa ataattcgtg gcttttgcga tcccattgtt ttaagagacc tttctttgtc
     9601 ttctccatcg taatacctga tccgtcgtat cgttttaagc gcgcattttt gagtacaaca
     9661 tttttaatca aatgttcccc ataaatctct tgggcttgtt tatacatttc ctgatctact
     9721 gcgtcaccgc tttccaacat gaaaggaata acaccagcaa catataaatt tgtatcaaat
     9781 tctttcacaa aaaagtccat atactcaaga tattgcatcg catttctcat cgattttact
     9841 tgggtctgta agacgacgat tgcccaatcc gcaatgtaca tcgcggattt actatataca
     9901 gaaatagaag gtgggacatc taaatagatg acgtcaaact catcaaataa tggttctagc
     9961 ttttctttca tatatttgaa ttgggataat gaatcattat attggtcttt gatccaatca
    10021 tcatacattt caaaggacat gtctgctgga agtatataga ggttattgat tactttatag
    10081 acagcctctt taggtggaag attgtttatc aacacgtcag tcacagtatt ttcgtaaaca
    10141 atcggttcac cttctccgcc tccttcttcg ttagtgaagt tcatatgcgt taagttcatc
    10201 acatcagaag tatttccttg catatccaag tcaattatca aggttctttt tccttcatta
    10261 actgctgatt caaaccctaa catttgcgcg cacgtacttt tacctacgcc acctttaaaa
    10321 ttaccgatta catatttctt cgctaccacg ttcctacctc ttttctttta gcaatattca
    10381 tttgatacta ttataacata tttattttta gttactacca aaaaaaagat atatatgtac
    10441 taccttataa ttaataagta aaaggtagta cataaataag gaaaagctga aatatattga
    10501 tttatagctt tcctcaaagt aatgacgtta tttaatttat cgagaagcag agaatactcg
    10561 cgactttagt catgtagacg aatcagattt tcgataaaaa agaagccgtc cttcttaaga
    10621 acgacaacaa atcactagat taattttttc aactgttcat aaaagttttc atgcgttcca
    10681 cataacaata ctggaataac cttgttttct ataatctcgt aagctactct atatgttgtg
    10741 cccgcatatt tgaacccact agaccaaata cctgataaat ccccagtttt ttgttcaccc
    10801 ttatatggat caatcgcaat ttcatcataa attaaattca aaaatttttc tttcaatggc
    10861 ttctctttta gcttttttaa atacttaatg acatgttttg aaggaacgat ttcataagcc
    10921 aatttctgtt gccaactcct ctttagtcat tactttagaa ttagtaagtg tatcttcagc
    10981 aatatctcta aaggaagaat gcattttgtt ttttctattc ttaaattcta caagaatctc
    11041 atctttatcg tagccttctg caattatatc agacaaaata tcttcgctaa aatcataaaa
    11101 ttctttttgt ggttcaacaa attcataaag aataccattc tctaccaact ttgcttcaac
    11161 ttcgactcca ttaggaacat taaaatcttt aggtactgtc aacatgattg aattgccttg
    11221 ttttcttgtt tttgtaatca tttaaatccc tcatttctaa cagtatgtaa ttacttagta
    11281 aatacattat accacgagat aatgtattta caagtaatta ctgtgactcg taatgcgtcc
    11341 ttgctgaata aatttcaacc acgtgttcat cttcatctac cttataaaca acccggtgtt
    11401 gtctattcaa tcttctaaaa tacctacctt catgtgtagg ttttaacttc tcaaatgatt
    11461 gggttctgtt gcaaagtttt aaataaagaa taaaatccct tacggtatct atgatttaag
    11521 ct
//
LOCUS       DACDYI010000085        11428 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.085, whole genome shotgun sequence.
ACCESSION   DACDYI010000085 DACDYI010000000
VERSION     DACDYI010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.085"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(368..1486)
                     /locus_tag="IWM52_002460"
     CDS             complement(368..1486)
                     /locus_tag="IWM52_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974678.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1957..3264
                     /locus_tag="IWM52_002461"
     CDS             1957..3264
                     /locus_tag="IWM52_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP9974679.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3383..4582
                     /locus_tag="IWM52_002462"
     CDS             3383..4582
                     /locus_tag="IWM52_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP9974680.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4609..5877)
                     /locus_tag="IWM52_002463"
     CDS             complement(4609..5877)
                     /locus_tag="IWM52_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAP9974681.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(6040..6686)
                     /locus_tag="IWM52_002464"
                     /pseudo
     CDS             complement(6040..6686)
                     /locus_tag="IWM52_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7094..7597)
                     /locus_tag="IWM52_002465"
     CDS             complement(7094..7597)
                     /locus_tag="IWM52_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9974682.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7720..8484)
                     /locus_tag="IWM52_002466"
     CDS             complement(7720..8484)
                     /locus_tag="IWM52_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAP9974683.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVISLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8592..8867
                     /locus_tag="IWM52_002467"
     CDS             8592..8867
                     /locus_tag="IWM52_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAP9974684.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9305..10255
                     /gene="yidC"
                     /locus_tag="IWM52_002468"
     CDS             9305..10255
                     /gene="yidC"
                     /locus_tag="IWM52_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP9974685.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(10434..10634)
                     /locus_tag="IWM52_002469"
     CDS             complement(10434..10634)
                     /locus_tag="IWM52_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974686.1"
                     /translation="MGGISALSFMENGVLCGRATWAKGVAPFVTEGEEKASSWMLEEG
                     KQNIKELSTLLEETATPVYVDR"
BASE COUNT         3441 a         2397 c         2018 g         3572 t
ORIGIN      
        1 tttttaaacg gtgttccaga agtagcttct tcggaaataa gccgaaattt cacaaaaatt
       61 tgagaaataa tttttgtcaa tctcttctta tttcttgaag ctgaccactt ctgtcacaac
      121 ctcttcctta aacggtgttc aaaagcttgc atctgcggta ataagcccac caaacaaaaa
      181 atatgaagag ctatttttgc ttgctgttct tattacttga tgcagaacag ctttttcaca
      241 acctctcata cacggtgttt aatcagctga ccttcggtat tagctcaaaa aacggaaaaa
      301 tatgtgaaac tatttttccg tttgtttctc taatactcag gtcaaaacac tgatttcaca
      361 acctctttta ataacgaaga atcgctgtca gactcttttc atctggcgca tctttttgat
      421 ccaaaataaa cactttcccg ccattaatga tcacatcatc tgctaatgca ttcagcactt
      481 gacgccgatc atattctgtt tcaggatctt caccgaaacc atcagctaag tttgacgtag
      541 cgataaataa atgtgacact tttccttctt tcgcagctgg aacgatatca actaactgat
      601 cgatgaattt cttatctaag agtttattgt attcttttga ttcttgagct gcgagttcac
      661 ttgacagctt cttcgcccct tcttcgatat ctctaaagga tagctgcgca ggagaagcag
      721 aaatcgcaac gtctgcacaa taatagggat ttttagcaat ttttttgaac atcgtctgat
      781 tttctggtaa tgcaaataaa taaatcggcc attgttcagg attttcgaaa gaatccttca
      841 agtacgtatc aacagcttgg taatagttga cccaatcgat ctctacttct tcatcttttg
      901 gatttacgcc atggaaagtt actccatctc gggctccgcc tcctccatta gaagagaagt
      961 tcaaaccgcc acctgtcagt tcagatccca atgcagtaac gacatcaacc ggtgcccctt
     1021 ctggtagaga tacttctttt acttgtttgt tgctgacctc atacaatttc atggagtcgc
     1081 ggttcaatcc caataaataa taagaatagt tgaactgccc atttttgata atagccagta
     1141 aataaggaag atcacttaca tagtattgat cgtctaccgg gatactcaaa cgatggatat
     1201 aggtctggtc agcagttata atgaccgcta cactcgtagt tgcactacgc caaaacgaag
     1261 gatctcctaa caaattttca attttttctt ggaacgggga ccagtcatgt tcggaatatt
     1321 ttttctcaaa acgtttcttt gcttctttcg caaaattttt gaatttgatc tgatcttttt
     1381 ccacatcttg atgggcgaca tgcgtattca gcatgatcgt gacgaatggt cctttagcgc
     1441 cttctgaagt caattgtgtc aataattctt tatccttttt tgccataaac gtacccctcc
     1501 taaaaaaatc attcctttgt ttctatctaa agtctaccac aacactattt ttccatacaa
     1561 taaaacgtat ctaaaaaaat aaaacggttt ctacggtttt aaatttagct atttttttaa
     1621 gttatcttca taatttcctc aattatttag ccccagtctt caactattct tcatgaaatg
     1681 ctgataaact aagaaaaaga ataaaagagg tggatcttat gcatttgatt atgattttca
     1741 gtttattaag tattatcagt ttatttgctt tcagcgcttc ccgcttatta gatgcggaaa
     1801 cacagcctat tcctgtaaaa gtacgtaaaa acgaccgcta aacttccata acgcgcgttc
     1861 ttatcctaaa gttttttcag aaacattgaa actctctccc ctctctgata aaattttaat
     1921 ttgtagcgaa acaaattaat tacaaggaga tcggatatgt ccataaaaaa aacacgtcta
     1981 gtcctattat tggctagtct cattctttgc cttgcttctt ttctaccagc tgttgctgtg
     2041 caagctgaag atacgttcaa agtaaatgca aaagcagctt ttgctgtaga tgcgcaaagc
     2101 ggtaagattc tttatgatca agatggagaa aaaaccatgg ggatcgcttc aatcacaaaa
     2161 atcatcggtc tttacatcgt ccttgatcaa gtaaaagaag gaaaactgtc atggggtgat
     2221 aaagtttcga tttcagatta tgcagaaaat ttgagtatca ccccagattt atcgaatgtt
     2281 cctttgcata aagaaaacac ctacactgtc aaagaattat tcgactctgc aatcatccag
     2341 tcagccaatg cttcaatggt ggccttggca gaaaaaatct ctggcagcga agctaaattc
     2401 actgaacgaa tgaaagaaca attgaaagat tggggaatca aagatgcaac aatcgttaat
     2461 gcatcaggat tgaacaactc ttatcttggc gaaaaccgac cagaaggcac tggagaaaac
     2521 gacgaaaatc aaatgtctgc tcaagatgtg gccatcgttg cccgccattt gatcttggat
     2581 ttcccagaaa tactggatgt atcaagtact acgacccaaa tgtttggcga aaatacgcaa
     2641 tcaccagtgg aaatggtcaa ctggaactgg atgctcccag gattcgttaa ttacaaggaa
     2701 ggcgtggacg gattaaaaac tggtacgact gagcttgcag gtgcttgttt cgttggtacg
     2761 atcactaaaa acgatcaacg gatcatcact gtcgtattga atgcggacgg ccatgcggaa
     2821 aacccgagtg cccgcttcaa tgaaacagac cgtttgatgg actactgcta tgacaattgg
     2881 tcagaaaaag agctcggaaa agctaacgca agtattccta gtctaaaaac cattgatgta
     2941 aaagacggaa aagaatcaac agttcctgtt gtgcttaaaa gccctgtaaa agtatgggta
     3001 cgaaacgata tggatactgg caaattaacg atcacgccaa cacttgacaa gaagcaagtc
     3061 actgacaatg agatccaagc acctatggat aaaggtacaa aaatcggtac cgctacgatc
     3121 accttggcag acgataaatt aggctattta gaagacaaca atgcaccatc aacagagatc
     3181 attacggata aaacagtgga aaaagcgaat atattcgtga ttggctggcg acgcgtcgca
     3241 gaatttttcg gcaacttgtt ttaattgaac aataagaaaa acatctttcc atcggaaaga
     3301 tgtttttttg ctttttctag tttaaataac agcttttttc atctattttc gttatactgt
     3361 ataagaaaaa ggagttgaat ttatgaatct aaccagagat ctgatctttt tagttctcta
     3421 tcttattggg gtattctcta tcgtttattg gcgaaaacag aagaaggatc cttccttgtt
     3481 cttttacggc agtacgtgga catttacggt aatcttaact tattttgtcg tgttggaaat
     3541 tttttattcg accttttatt ttttgattcc attgttgttc tttagtatct ttgctttttc
     3601 ttatttcaca gaaaaacgaa ggctactgaa tggtctttta ttcaatgttt tactcatcag
     3661 tttcggcatc tacttgtttg tcttgctgta tgaaacgcaa aatatttttc ttggcggact
     3721 gatcgccttg atcacgattc cattgctact tgtcctttta tttggtattt atggattgat
     3781 tgtttttctt ttttggaacg gggtcacagt tttacgtaga gaatcgcatt cattagctaa
     3841 tcttttaacg ttgatactag ccatttttct tacactgttt cttgtttttg actttttctt
     3901 gttaaaatat ttgccgcaat ggataaatgc tttgtttttc tgcgttcccc tgatcttgat
     3961 ctatctattt atcgtattct acaacttttt gactgtctca ttcttgtatc agttcaaccg
     4021 gcctcgatac aatcaagact tcatcgttgt gttaggcgct ggattgatca atggagaaac
     4081 agtttctcct cttctagcca agcgaatcaa taaggcaatt gctttttatc gggctcaaag
     4141 ccgtgctaca ttaaatccgc ccatcttatt gatgtctggc gggcaaggag cagacgaaaa
     4201 agtaccagag gccattgcga tgaaacaata tgccatggaa caaggaatcc ctgaaagaga
     4261 cattcttgtc gaaaccaatt cgacaaccac tttggaaaac atgctctatt caaaggaaat
     4321 catggatcag caaatgaaag ggccataccg cgcgatcttt agttccaaca attaccacat
     4381 ttttagagcc ggtctttatg cgcggcaagc caaattgaaa gcaaatggta ttggtgcaaa
     4441 aacagctttc tactacttgc ctaatgcgtt tttaagagaa tacctcgcca tcttgatgat
     4501 gaacaaaaaa agacatatga tcatttgcgg attgcttttc gcaggctccg cattcttatc
     4561 gctgattact ttacttttct aaaaaaacaa ggtgtgacaa aacatatgtc acaccttgtt
     4621 tttttctttc attctcttaa ctattggcaa aaagagtatc ccttttttga ccgctacttg
     4681 ctgagcagaa taaataccgc tgttgcctga acacataaag ctgatcaagc agatcataaa
     4741 gaagtatact gccgcttgtg aaccgaacaa ttcgatcccc atcacaaagc aggcaatcgg
     4801 tgtatttgtc gcaccagaaa atactccgat aaagcccaat cctgcaagaa aaggaacaga
     4861 aacatggagc aaaggcgcta gtgctgcacc aagcgttgca ccaatttcaa acaatggtgt
     4921 cacttctccc ccttgatatc ccgcgcctaa agacaaaact gtgaagaata acttcccaat
     4981 aaaatcatag acatgtgcct gtccattaaa tgattcttga agtaacggaa gactcaatcc
     5041 gagataacgt tgtgtttgca aaatcaatgc tgctgccaca actacagctc cacctatcgt
     5101 atttcgacaa acaggatgag gaatccattg tgcatatcgc gcttttaaaa agacgatcga
     5161 acggctgaat acccaaccaa tcaaaccaaa agcaatccca gcaacaaaaa ctttgaagaa
     5221 cagcaacata ctccattcag gaatcacacc catttggtaa tgtagatgag aaacaccata
     5281 gctttctgtc atgaaatttg caaacagtgc agcaaagaag ctagggaaga tcgcttctgt
     5341 tcggactttc ccgattgcca acacttctaa tccaaataat gttccagcta aaggtgtgcc
     5401 aaatacagaa ctaaaacccg cactgattcc actgataatc aagatttccc gttctgattt
     5461 atccaatcgg aacaaacgtg ccgtattatc tgctaacgct ccccccattt ggactgctgt
     5521 tccttcacgg ccaacagatc ccccaaataa atgggtagtt atggttccaa acagtgtaag
     5581 tggaataagc cgcaacggga ttttttcttc tccaccattt ccttgatcga tcactaagtt
     5641 gtttccacga ctagaaagtc cgccgtgata cgcatagaga taagcaaaaa gcgcaccact
     5701 aactggtagt agacaaagga gccaaggatg tgatagtcgg atatctgtca caactgtcag
     5761 gctatttaag aaaaaagcag acaagctccc catgaatata ccgagaatcg ctgtgatcac
     5821 cagccacttc agcatataca ctaaaatgcg tactggactc ggtaattgtt tttccataaa
     5881 aaattcctcc tcaataaaaa aacgcctact aaagaaaggg gctgtcccca gactttagta
     5941 ggcgtcatta gctatctttc gttagcattt aaaggcgaac accatcacct attcacttcc
     6001 attaaaagat agcatatttt tctgtaaaaa acaataaaat tatcgttctt tctttacacg
     6061 ttcagcaaat tcatagaaag gagccaattt cgttaactgt tgattacatt ggtcactatc
     6121 atgacaaata taattcccct ttttcgtata cgtgccatca gaacctgctt tcgttgtaga
     6181 aagaaaaagc gaaacattcg atgttttctg acagattgca cagattccct tatttatcgt
     6241 tggtgataaa gtaccgctaa tcccttttaa tttttcatct tcgtaataga ccatgaattt
     6301 tttctgcgta cctggatcat tccagccgat gtacgtatgt tcttgcaagt cgatttcatt
     6361 ccaagctggt tgtttgagtt ttttcacttt tcggaataat ttctcgattt gtggtttgct
     6421 aggctgcata aacggctgaa cgatttgctt cagctcctcc aatgctcttt ctgcttgcgc
     6481 ttttgtcagt tttacgtcta aaactgtcgt taaaaatgta tcgattctgg cagttcgtga
     6541 tctaatatgt ctcgaatctt ttcagctgct agtgcctgaa ctgtttgtac cgtttttgga
     6601 tcattgacag atgagtacgc atttaataac tgttctactt ctcttattat aaaaaaatat
     6661 tggtatggtt ttattgtttg ttccatgatt ttaaagtcct ttcttctaac caaagtgcat
     6721 actttgtaga aaaaagtgat cttctgcaaa gtatgtgtta cctgaagatc ccggactctt
     6781 ttaaataaga tacgttttgc atcttatcat cacccccttt aatgcttatt attctatgta
     6841 catgattcat tcgataaata aaagtaaacc gtatcttgaa cagatagtca actgcaaaca
     6901 gaaaaaagga ctgtgacaaa agtcgtgtca aagtcccttg tttcgaatga atggtgttca
     6961 aaagctggca tctgcggtaa taagccgaaa cttcacaaaa atttgagaag caattttcgt
     7021 aaattccttc ttatttcttg aagctgacca cttctgtcac aacctcaata tggtctatta
     7081 ttggttttat ctattaaatc gaggattctt tcccaaagta acgttgccat ctcttacgca
     7141 tacttgctga cataggctga gcggcttctt tgactgcgca gtatttgtct acgaatgtta
     7201 cgatatatcc ttctttgtac ttaggcggtg caatcgttgc gccccacatg tgcttcaaaa
     7261 tgatatctcg ctcaagatcc gacaattcgg tgatcttttc tgcattcttt acagcaattc
     7321 ttggatggat atacgcatgt gtgccttcat caaatttcgt cgtacgccaa tcatagtaga
     7381 acagatcatg caaaagtcca gcacgcgctg ttgctttagc attgccaccc catttttttg
     7441 cgagcagata actatagtaa gaaacactga tggaatgctc caaacgagtg gaatgaacat
     7501 gctgtgtgta attcgctaat tttttcactg cttctgtttc taaaagatct tctacataag
     7561 acatatattc ttcgtcttca cgccattttt ctgtcataat tatcgatctg ccaccttttt
     7621 gaatttcatt ttcttctgaa attgatctaa gtataccacc agtgttcgga ttattctagt
     7681 cgccttggat aaaatagcca aagcttaata gttccttaat cataaagaaa aatgataaag
     7741 aacaatccct gccgcaaccc caacattcaa cgattccgca tttcctttca tcgggatata
     7801 tacgttctga tcagtcaaag acaagatttc ctgacttacc ccctgacctt cgttccccat
     7861 gattattgcg aagttatctg atttttctac tttgtctaat gcgtgtgctt cttcatttaa
     7921 ttctgtgcca tacactgtaa accctttgtc tttcattaaa gaaatcactt ctgtcaaatc
     7981 tttttgcaac acaggcaaat gataattgct tccctgcatg ctgcgcagca cctttgtact
     8041 ataaatatct gccgttcctt tgcctaataa aacaccagcc atatcaaatg catctgccgt
     8101 acggatcatc gtccctacat ttccaggatc ttgtacatta tctaacaaca gccatcctcc
     8161 tgaaaaatct ggcgtgcctt cagccgttaa cgccatcact gctaagattc cttgtggtgt
     8221 aggtagatcc gatacagcat ccatgacctc atccgaaact agaaaagtag aaatctcttt
     8281 gtttgcggcc agccagtctc cccattcttt catgcctcgc tgtgtcacaa aaagttcttt
     8341 tatttctgca tcagacgacg cagcttcttc aatcagatgg aacccttcca aaagatattc
     8401 ttccttttct tcccgatatt tctttttatg tagttttttt aattctttga ttcttggatt
     8461 ttttgctgat tggatctctt tcacattcct cacctcccca tgattatatc atggaatagg
     8521 tgcttgagct tgtttcatcc taaaaaaatc ggtatcatta atttataaaa taggaaagaa
     8581 aggtgacgaa catgcgcaaa gttaaaatga acgtccaagg acgcgtccaa ggcgtaggat
     8641 ttcgctacat gaccaagatg gtcgctgatc aactaggcgt gactggaacg gtaaaaaacg
     8701 aagatgacgg ctcagtcacg atcgaagcaa tcggcaatga cgacatcatc cagaaattta
     8761 ttgaagaagt caagaaatct ccgagcccaa gtggacgagt gcagtatgtg gatgtccaag
     8821 aagacccgat gattgaggaa cgaaagaaat ttgatgtggt gggataaggt tgtgaaaaag
     8881 ctggtctgca tcaagcatta aggacaggtt gtgaaaaata gctctccata tttttcaaca
     8941 ggctggactt aatgccgtag atgcaagctt ttgaacaccg tcgataaggt tattcttgag
     9001 gcgctttgcc tcaaaaatta aggacagcag tgaaaaagct gatctatcac ttgcagtgag
     9061 ttcatttgac tccgcttttt cgggcagaaa aaaagttttt agtttattaa agtaagaaag
     9121 aaacacttgg aatcattcta ctttttccta gtatttcttt cttgattttt tgtctttttt
     9181 tcaaaatttt tcgctacgta aaagaacgaa tatttatatg aaatttctat gaagttgatt
     9241 gaattccatg cgctttttta gtatagttaa gtttgtgtaa aaattataga agaggaagta
     9301 ttctatgaat aaatggaaaa actggttatt gggatccgga cttgtcagta tcctgctctt
     9361 tttatctggc tgtgtgcgga tggattcaaa cggaaaccct gacacttcgg gaatcgttta
     9421 tcgagtactc gtccatccta tgggccaagc gattacatat ctagttgaaa atttcaattg
     9481 gtcttatggc tgggcagtta ttgcaatgac tgtgatcgtc cgtatcatca tcttgccact
     9541 aggtattagt caatcgaaaa aaacaatgat ccaatctgaa aaaatgcaag cgttaaaacc
     9601 acaagttgaa gcagcccaac aaaaattgaa agctgcaaca acacgtgaag aacaaatggc
     9661 agcacaagca gaaatgcagc aagtataccg cgaaaacgga ttgagtatga ccggcggaat
     9721 cggctgtctg cctctattga tccaaatgcc gatcttctct gccttgtact ttacggcacg
     9781 ttatacagaa ggtatccggg aatcatcttt ctacggcatc gatttaggct caccaagtat
     9841 ggtacttgtt gccattgcag gggtcgctta tctgcttcaa ggatacatct caacgatcgg
     9901 tattcctgaa gaacagaaaa agacgatgcg tacgatgctg atcgtctctc ctgctatgat
     9961 cgtcttcatg tcgatcagtg cgccagccgg tgttacactt tactgggtag tcggtggtgt
    10021 gttcagctgt ctgcaaacat tcatcacaaa cgttatgatg aaaccacgtt tgaaagcaca
    10081 agtggcagaa gagttgaaac aaaacccacc aaaacaagtc gtcactccaa gaattgatgt
    10141 gacagaatca gctaaaccag ctgacagcaa acaagcaacc aaacaaatca agcaacccaa
    10201 aaacagtaca aacaataaag gccgcaacgc aggaaagcag cagaagcgga agtagatagt
    10261 aaataagagt gtaataaaaa ttcattaaca gttttagcaa ttaaaaatat cgaccaagat
    10321 ttttgaaaaa gaaatctggt cgatattttt atttaagtga atgtttaatt tgaatcatcg
    10381 attgatcgct atctaattgc agcagcatct ttttatgaag aaacaacttt ttcttatcta
    10441 tcaacgtaga caggtgttgc tgtttcttct aatagtgtac ttaattcctt aatattttgt
    10501 tttccttctt ctaacatcca actactcgct ttttcttcgc cttcggtaac aaaaggcgcc
    10561 actcccttcg cccatgttgc tcgcccacat aagacaccat tctccataaa ggacaacgcg
    10621 gagataccac ccatctaaat aacatccata aacctttcta gtagaagaat agtgatagta
    10681 tccatccctt tttctactgg gaatacaatt acacgattca aagcttgata cacaacattt
    10741 cccttcttat aaaccgctat tgtaaccgaa ttgttttgaa tcaccttttt gggatagaaa
    10801 ctggctcctt tccgcaactt cttaaatgtt ggtttctttt atactttctt tgtagcaact
    10861 acaaatttct tcaaatctct aaatgtttgc ataacaaccg cctttctgac ttttactcag
    10921 gatggtattt aaaaatcagc aaaataaggc ttctctccat cttttttatt tcctagctgc
    10981 tttaaagaca ctcttgctgt tttttaatct ctatcaaggg ggctctgaat ctattttgat
    11041 gaacttatct tattaatttc tcaaatgagc tgtcagacac aactgtattt tttcaatctc
    11101 ataccggaca actatgccag cttaaaaaaa tttcctctgg caaaacttct ttattgaaaa
    11161 atgacaatga aaaaagacca ttctttttgt tgaatgctca ttgtgtaaac atatttcctg
    11221 cgactgctct tcgaaccatt ctcagtgcaa tccctaattt atctattaaa tcttctttta
    11281 gttcctcatc tttcatattc aaaatttcct ttgtatgtta cacaagtttg ttctctaaat
    11341 aacgcgaatt gtaaaattaa gttagaaaaa taaaaaggca tttatggtac actcaaattg
    11401 tatttccgac gaaagaaaac aaaggagt
//
LOCUS       DACDYI010000086        11329 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.086, whole genome shotgun sequence.
ACCESSION   DACDYI010000086 DACDYI010000000
VERSION     DACDYI010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.086"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..1030)
                     /locus_tag="IWM52_002470"
     CDS             complement(<1..1030)
                     /locus_tag="IWM52_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9974687.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1112..1801)
                     /locus_tag="IWM52_002471"
     CDS             complement(1112..1801)
                     /locus_tag="IWM52_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP9974688.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="IWM52_002472"
     CDS             complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="IWM52_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAP9974689.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2328..2831)
                     /locus_tag="IWM52_002473"
     CDS             complement(2328..2831)
                     /locus_tag="IWM52_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAP9974690.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="IWM52_002474"
     CDS             complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="IWM52_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAP9974691.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="IWM52_002475"
     CDS             complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="IWM52_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAP9974692.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4799..5467
                     /gene="sdaAB"
                     /locus_tag="IWM52_002476"
     CDS             4799..5467
                     /gene="sdaAB"
                     /locus_tag="IWM52_002476"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAP9974693.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5480..6352
                     /gene="sdaAA"
                     /locus_tag="IWM52_002477"
     CDS             5480..6352
                     /gene="sdaAA"
                     /locus_tag="IWM52_002477"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAP9974694.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6443..7462)
                     /locus_tag="IWM52_002478"
     CDS             complement(6443..7462)
                     /locus_tag="IWM52_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP9974695.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7635..8522)
                     /locus_tag="IWM52_002479"
     CDS             complement(7635..8522)
                     /locus_tag="IWM52_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9974696.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="IWM52_002480"
     CDS             complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="IWM52_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAP9974697.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9245..9415)
                     /gene="secE"
                     /locus_tag="IWM52_002481"
     CDS             complement(9245..9415)
                     /gene="secE"
                     /locus_tag="IWM52_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAP9974698.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="IWM52_002482"
     CDS             complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="IWM52_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP9974699.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9753..10397
                     /locus_tag="IWM52_002483"
     CDS             9753..10397
                     /locus_tag="IWM52_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9974700.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10453..10956)
                     /locus_tag="IWM52_002484"
     CDS             complement(10453..10956)
                     /locus_tag="IWM52_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP9974701.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10969..11015)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
BASE COUNT         3307 a         2356 c         2071 g         3595 t
ORIGIN      
        1 atacagtatt atttcggttg cttgctgctt cacctagccc ttgtactcgt gtttgattgg
       61 cgacttgacc aaattgattc ccgacgtcta ataaaataat agctaaaagc aaaatggcca
      121 aatggcttcc gccaaccaat aaagcaataa agcttattgt ttgcataaca agtcccatat
      181 acacgatttt tctttcagaa taagaatctg ccagtcgtcc cacgataggt gcagcaactg
      241 ctccagacaa accaaaaatc gctaatatcc ctgcttcgaa tgttccccaa ttgtaggcgg
      301 gactgctgat ataaaaaatc aacgtagacc aaaagatcga aaatgttcca aacatgaaaa
      361 atccactgac tgccgcttct cttaatagac gttgagaagc aactaaatgc ggcaaacttt
      421 taagtgaatc aaggtatgaa acagctgtac tactttgctg gacagaacgc ggcattttcc
      481 agtaaagcag aagtgtaaga attccgactg cacataaagc aaataaatag atcatccgcc
      541 aagaaaaaag actggcaaca ataccagata ccgtccgaga aagcaaaatc cccacgagta
      601 aaccgctaag caatgtaccc atgaccttcc ctcttgcttt tggtcccgct agtaccgcac
      661 cgtaaggaat gatgatttgc ggcacgatcg aaagtaatcc tattaaaaaa gaagcgcatg
      721 caaataacgt aaacgaaggc gcaaaaaaag cggccaacag agagacggca gataacgcta
      781 ccattcgaat gatcaatttt ggacgatcca ccacgtcccc taaaggaact aaaaacagta
      841 accctaacgc atagcctaat tgggtcaaca tcgtcaatat cccgactgca cttgtgctaa
      901 cagataaact agctgctact tttgttccaa tcggctggat gtaatacata ttggcaacaa
      961 caacgccaca agtgattgat aatagtaaaa taagcgaagt tgttaaaggt gttttttcat
     1021 tactcttcat tgatttcccc catttcctat cttagtttac aacttatgga aaagattgga
     1081 aatagaaaaa tacaactatt cgccacactt attatttgtg cttttgtttt ccttgttttt
     1141 tcttgcgatc tttataaatg aagacaacta accctactag cacagtaaca cttgcaacca
     1201 acgtgactgc agttttgtta tatccgtgaa acagagcttc tccaccacac gcatacaaca
     1261 cagacgaagg gatgactcca atcacgatcg acaatatgat ttgctgtaga ggcaatttca
     1321 tcgcatctgc agcaaaattg accatagaag atggaataac cggtatcata tagcctaaca
     1381 ttacacctat ttttggatgt ttcatttgtc ggattgcttg cacccaatga ttcgtttcgg
     1441 ttttcttatc taaaaatttt aagcgatgca taaggaaaat tgcagtgatg ttaccaagag
     1501 cattacccaa aatgttgatg atcgcacctg tcagtggtcc aaagctcaaa ccagcaatga
     1561 ctccaactac tgaagtagga atacctggaa cagcacacat caccgctgtc aatacgatca
     1621 agagtatcga tgcaaaagtc ccatgcgaac gaatatgtgc gagtagctct gccttactcg
     1681 ctctcggatt taaaaataat tggatgtctg tctgatattc taaatacaac cgatagccca
     1741 ctaccagtat cagaatgatt cccaacgaga tcaacatcaa tcggattatt ttctttttca
     1801 tatttcctcc tgaaaaaaat ctatattcat taaataatag tttaacataa aaacaaaaaa
     1861 gcaccgaaat ccaacatagg atctcagtgc ttttaaatag taaaaattat ttaacagtta
     1921 cagatgcgcc aacttcttca agtttagctt tcaattcttc agcttcttct ttagatacgc
     1981 cttctttaac tggtgcagga gcgccatcaa ctacagcttt agcttctttc aagcctaaac
     2041 cagttgcttc acgaactgct ttgataactt taactttttg gtctccagct gcagttaatt
     2101 ctacagtgaa ttctgtttgt tcttcagcag cagcagcgcc accagcagca gctacagcta
     2161 caggagcagc agcagatacg ccaaattctt cttcaatagc tttaactagg tcgttaagtt
     2221 ctaggatagt tgcttctttt aactcagcaa cgatgttttc aatgttcaat gccattataa
     2281 tttcctccgt ttaattgttc ttttttattt aatgctttat gcggcaatta agctgcatct
     2341 ccatcttctt tgtctgccac tgctttgaca gcgtaagcca cgttgcggac tggcgcttgt
     2401 agtacagata gcaacataga tagaagtcct tcgcgatttg gcaattttgc caatgctgtg
     2461 atttcttcca atgaagagac tttaccttcg atgataccgc ctttgatttc taatgctttt
     2521 gcttctttag caaattcgtc catgattttc gctggtgcga ctacatcttc gttactgaat
     2581 gcaacagctg taggtccagt gaatacttcg tccatgcctt caaggccagc ttttttagct
     2641 gcacgtgaaa gaatagaatt tttgataact ttcatttcta cgttagcatc acgaagttgt
     2701 ttacgtaaac gtgttacttc atcaactgtt aatccgcggt agtcaacgac tactactgat
     2761 gcagctgctg taaatttttc gcttacttca tcaacgattg ctgctttttt tgcgataatt
     2821 gcttcactca tttttgagtt tcacctcctg aattttgatg gaagagtttt ttgcactgaa
     2881 aaggcaagtc acttttatag ttactttgct caaccagtat tattcatcac ctattcactt
     2941 cgttcatagt gattctactc gctttgctcg aaaggaaact cctttcgctt tgctcaacca
     3001 gtattgttca tcatctcttc acttcgttca tagtgattca caataaaaaa ctctatgcca
     3061 ccgcagacat agagggacta ttgaatatat tcaataaacg tcctcggtag gaaattaagg
     3121 cttgatgcca cctactgtct tcggtacaag ttaattatta acctcaacta gcttagcatg
     3181 ctaaactagt tgagtcaaga attatttttc agttggatca atcaacattc taatatttaa
     3241 ttagaaagaa gcttggtcaa catggatacc aggtccaaat gttgttgtga tcgttacatt
     3301 tttcatgtat tgacctttag ctgctgatgg tttagctttt agcaatacat catggattgc
     3361 tgcaaagttt tcaactaatt tttcatcgtc aaatgaaact tttccgattg gtacatggat
     3421 atttccagct ttgtccacac ggtaagttac tttaccagct ttcacttcgt ttactgcttt
     3481 tgttacgtcc attgtaactg taccagtttt agggtttggc attaagcctt ttggtcctaa
     3541 tactcgtcct agacgaccta cagtagccat catatcaggt gttgcaacaa ctacatcaaa
     3601 gtcaaaccat ccgttttgga ttttttgaac catgtcgtcg tcacctacga aatctgcacc
     3661 agctgcttca gcttcttttg ctttgtctcc tttagcaaaa actaaaacag tttgtgtttt
     3721 accagttccg tttggtaata caacagcacc acggatttgt tggtctgctt ttttagggtc
     3781 tacgttcaaa cggtatgcaa cttcaactgt tgcatcaaat tttgcaatgt ttgtttcttt
     3841 tgctaaagct actgcttcgg caacaggata aactttactt gaatcaactt tttttaatgc
     3901 atcttgcatt tttttgctct ttttagccat tttggttcct ccttgattgt ggttataacg
     3961 gttgtacctc ccacgtgttc tatatctttc aatatagagc caactgagaa gctgctgtct
     4021 tagggtgtat agattattct acagtgatcc ccatgcttcg tgcagttcct tcaaccatgc
     4081 gcattgctgc ttcaacgtta gctgcgttta ggtcttccat ttttaattca gcgatttctc
     4141 ttacttgatc gctagaaact ttcgcaactt tatttttgtt tggttcgcct gaaccttttt
     4201 cgattttagc tgcttttttc aatagaacag cagctggcgg tgtttttgta acgaatgtga
     4261 atgaacggtc ttcgtataca gaaataacaa ccggaatgat caaacctgct tgatcagctg
     4321 tacgagcgtt gaattctttt gtgaatccca tgatattgat acccgcttga cctagtgcag
     4381 gacctactgg tggagctgga gttgctttac ctgcaggaat ttgcaattta acgatttttt
     4441 ctactttctt tgccacgaga catacctcct taagtccgtg atgtggttaa ttggagatgc
     4501 atatttctcc tcccacgttt tatctgatct gaaaaaatcc agacaaataa tgtgcaaaac
     4561 aatgcacact ggtacagtat agcaaattta tttgtttttt caagattttt ttctgctatt
     4621 tttacatttt gcttgaataa acgctaaatt tattctaaaa aaacaacaat ttttcgttct
     4681 catttcttct tttatgttac acttgcgcta gcatggttca acagatgaaa gcaaacaata
     4741 acacggtttt ttagaccctt gtacaaagat tcctacataa caaaatgagg tggagaaaat
     4801 gaaagattta aggtatagaa gtgtctttga tattattgga cctgtgatga ttggtccaag
     4861 tagctctcac actgccggag ctgctagaat cgggaaaatc atacgtagta tatttggcga
     4921 acagcctgat tctgtagata tttatctata tgaatcattt gcaaaaacct atcgggggca
     4981 cggaacagat attgcattag ttggcggcct gttagggatg gaacccgatg atgaacgttt
     5041 agctgattct ttgaaattgg catatgaagc cggtatggaa gtcttgtttg ttcctaagag
     5101 tgaaaaagcc gaacatccaa actctgtaaa gatgcttcta aaaaaaggaa cacgaaagct
     5161 ttcagtcact ggcatttcga tcggcggtgg caatatccag atttctgaat tgaatggatt
     5221 caaaatttct ttaagcatgg gtactcctac cttgatcgtt gtccaccaag atgttccagg
     5281 tatgattgct aaagtgacta gtatactttc tgagaccaat acgaatatcg gcactatgac
     5341 agtgacacgg gaatcaaaag gtgaaaatgc cattatgata attgaagtag atgatcctca
     5401 agtagaagaa acggtaaaaa gactcaaaca gctgccaaat atcgacagcg taaattattt
     5461 cgattaagga gctaaaaaaa tgtttttatc gattgaagaa ttagtagatc aagcaaaagc
     5521 ttatccaagt gtagcagaat tgatgattgc agttgaaatg gaaatgagtg gccgttcccg
     5581 tagtcacatc atcgagatca tggaaaaaaa cctctcagta atggaacaat caatcaacga
     5641 gggaaaagaa ggtgttacct ccgtcacagg aattaccggt ggtgatgcaa aaaaattaga
     5701 tgattattta aatgaggcag actttttaag tggagaaaca atcttaaaag ctgtacgcaa
     5761 tgctgtagct gttaatgaag tcaatgcaaa aatggggttg atttgtgcta caccgactgc
     5821 cggaagcgcg ggtgttgttc ctggtgtgct gatggctgtc cgagatcggc ttgatttgac
     5881 acatgaacag caattggaat tcttatttac cgctggcgct ttcgggttag tcatcgcaaa
     5941 caacgcttct atcagtggtg cagaaggggg atgtcaagca gaagttggtt ctgcaagtgc
     6001 aatggcagca gcagctttgg tttgtgcaaa aaatgggact cctcatcaag cagcacaagc
     6061 agtagccatt acattgaaaa atatgatggg actgatttgc gatcccgttg caggcttagt
     6121 tgaagtcccg tgtgtcaaac gtaatgcttt aggctcttct caagctttta tctctgctga
     6181 tatggcatta gcgggtatcg aaagtgtcat tccgccggat gaagtcgttg ctgcaatgta
     6241 ccaagtcgga cgtcaaatgc cgtcgatttt caaagaaaca gcagaaggtg gcttagctgt
     6301 tacgccaact gctaaacgac ttgcggaaga gattttaaat aagagggcgt gataaagctc
     6361 cttaatttcg agtattaaga gattagttat cttcataaca aaaaacgaag agaggactaa
     6421 ttgcccttct cttcgttttt tattaatcca tcaatttcgt attttttact tcatcgatcg
     6481 tttttgttcc tgcaagttgc atcgtgatcg acaattcttt gttcaggtgt tcaaaaacag
     6541 atgttactcc ttctgcccca cctagattca acccataaat cactggtcgg ccaatcgcaa
     6601 ctaagtcagc accacttgcc agagctttga agacatgctc tcctcgacgg acaccagagt
     6661 cgaaaatcac tggtacacgt ttgttgacta cttcggcaat ttttggcaat acttcaaagg
     6721 acgcaggacc tccatctaat tgacgtccac catgattgga tacccagata ccatctgccc
     6781 ctgcagagat agcgattact gcatcttctg gcgattgtat tccttttacg ataactggaa
     6841 gatgggtgat ttctttgatt gttttgatgt catctggtgt caagccttgt tttgcagctg
     6901 cataaatttc tgcgattcct tttccttcgc cattaccact tgcactttgt tcgctatacg
     6961 cagctaagtt aggcattggt aatggaaatt ggaattgatt gatgacgtct tcttcacggt
     7021 aaccaccgag cgttgaatct gccgttaaaa tgatagcttt agcccctgct tctactgctt
     7081 tgttcaaaat gaactcattg aatctgtcgt ctttactcat atataattgg aaaaactgcg
     7141 gtgcgcctgg cgctgcatcg gctgcgtctt tgattgtcgt atttgcataa gtgctgatgg
     7201 aaaagattga tccagcagca gctactcctt tagctgtatc agcttcccct ttttcatgtg
     7261 ccaatccttg ggccgctgat ggcgcttgga taatcggtgt atctaattca attccgaaaa
     7321 cagatgtatg caaatcggct gagtcgattc cgcgtaatat ccgtggcatg attttctttg
     7381 tattaaaaga agtcgtattt tctttcatcg tccactcatc ttcagaacca ccgcgaatat
     7441 agccaaaagc tcctgcttcc atgcgctctt ttacacgacc ttctagttag gcaatgttta
     7501 caatatcgat tggatgttct tcattacttg tatgataagt catagtagat ccttctttca
     7561 tcttcttgat tacatataaa tcatactaaa cttacaaaaa gtaagcaaac atttactctc
     7621 agatactttt atcgctattc tttccaaata aatcgactta caagctgatc tacttcttgg
     7681 ttttcatgaa gcatactatg agaggcattt tcacctgtca ctactttttc ctctatttca
     7741 tttccgcgtt gcttcaacaa cgcatagacc gctaatgcac tgtttaacgg tactgttcca
     7801 tcactcaagt ctgtttcatc tagttgacca gcaatcaaga aaaatcgagt ggttatagga
     7861 atattaccga tcatttgctg gtaatcttga tagcggttag aaacgacagc aggccccttt
     7921 gctaactcat ctgtcagaga ctgcgcacta tcatcgacaa aatcattaaa aggcgcgcca
     7981 attgctgtta atgtctgaac ttgtggcaat gacgtatcct gtccatacgt tcctaaatag
     8041 cgtagtgcac taactcctcc catggaatgc cccactatgt tgactttatc tatgccgtag
     8101 ttttctttca gatacaaaag acaagttttg atccattctg cttgattcca ctcgttattt
     8161 ttattatctt caaacaatac ttggaccatc ggattgtctg cttttccaga caaatctcct
     8221 gttgcttgta tttgtccatc tgttcctact gttaaaacca attcttttac tgcttgctga
     8281 tttttttcca aacgccgcaa catcccttta aaggaatttt ctgtcccgct gtagccgtgg
     8341 aaaaaaagtg ttggaatagt ggttttttct atcactttac tggattctgc cgttttttct
     8401 ttatcagaaa cttcagtagt atttatcttc gtcgtctggc aaccacttaa cgcaaacaaa
     8461 ctgataagga ctaaagctgc tgcagttgca cgtttgcata aatttttgat tggttctgtc
     8521 atacacctct tcctttccat atagaaaaaa gaaagacttg aaaggccaat catatgatcg
     8581 acatttcaag tctctttatt aatcgatttt atctacttga tcgaaatcaa gttctgtact
     8641 ggtttcacgt ccaaacatat cgatgttgac tttcaatttt tctttttctc cgtcaacttc
     8701 tgttacttgt ccttctaatc cagcaaatgc gccttcgatg attttcacga cttcgccaac
     8761 ttctacttct agatcatttt gtcgcaggct cataccgata gaacgcaaga tatgattgac
     8821 ttcttctgga agtaatggtg ctggtttgct tccggctccg tgtgaaccaa cgaatcctgt
     8881 cacaccaggt gtgttgcgga caacatacca tgaagcgtct gtcatgatca tttcaacaag
     8941 tacgtatcct gggaatgttt tatgaacgat ttctttttct ttcccatttt tgacttcagt
     9001 ttctttttct tcaggaacaa cgacacggaa aataaagtcg gccattttca tactttgcgc
     9061 acgagattcg atatttgctt ttactttgtt ttcatatcct gaatatgtat gcaagacata
     9121 ccagtttttt tcaaaagatt ccattgaagg ctcctcattt cattgtaaaa taaaaaaacc
     9181 tcagtacacc cgaagttttt ctttcgcttc attatagcac taaaagtacg ttgtggctag
     9241 cgagttattt taaaatccag ccaaataagc tttggatggc tgtatccatt acgtaaaaca
     9301 tcaatccgaa aataatagaa gtttcgatga ctactaacgt atctttacgg agttgttttc
     9361 tgcttggcca agttacttgt ttgatttcat cttttacgct ttttagaaat ttcatgagac
     9421 aacctccctt gctactgttt atttcgtttc tttatgtaat gtatattgat tacaatattt
     9481 acaaaactta ttgatttcca aacgttcgcc gctttttcct tcactgactg attttgtgta
     9541 gttgcgagac ccacaaacgg aacaagcgag tgctgctttt ttctttgcca tttacttctg
     9601 cccccttgct gagaatcaga cttatacttc ataacagtat cacgtaactt tttacttgtc
     9661 aatttgtgaa ataaactatc tattgttttg gataaaatta tgatatgatt aatttgaaat
     9721 taagctaaaa tgagacaatt ggaggtatag cgatgctatt aaaaacactt gtatataaga
     9781 aaaaagatct gacaaccgtt acagagacta gtacattaga agaagcatta aaaattttag
     9841 aaacttccgg ataccgttgt gtgccgatct tagattcttc aggtaatatt ttccgtggaa
     9901 acatttacaa aatgcatatc tatcgtcata aagcaaatgg cggtgatatg acgcttcctg
     9961 tcactcatct tttgaaaaac gctacaaagt tcatttacct ggatacttct ttcttcaaag
    10021 tattctttac gatcaaagag cttccttata ttgctgtcct tgacagtgaa aatcattttt
    10081 atggtatttt gacacatagt acgttgctta atatgctctc tcaatcttgg agcgtagaac
    10141 aaggaagcta tgtactgaca attgcttcca caggaaagca aggagatttg gctgctattg
    10201 caaaaatcat ttcaaaacac agcagcattg ccagctgtat tactcttgat atcgaaaaag
    10261 acgaatacat tcgacgtaca ttgatcacgc taccggctaa cactgaagcg aaagtatgtg
    10321 aaacgatcgt caaccactta gagaaaaaga attataaagt ggtagaaatt gaaaatttgc
    10381 aaaaccaaga agcataatca aaaagcgaaa aaagaaaaca gctaatctgc tttctttttt
    10441 cgctttttat aattagacac gcttttcaaa atcaagcgcg cgatctaaat aatacatcaa
    10501 tggtgctgaa acagccatga cgataaactc acttaatgca agcgtgccat aggttgtcca
    10561 aaatgcttgg ccacctgttg gaactaacat ataagcaatc agtcccatgc tgatggtaaa
    10621 gaagatcgtg ttcaatacta aacgcatctt tacatttttc actttgtttt gcaataaagc
    10681 tgtcaggcct aaagaaagca atgtttggcc gccaccaaaa accacatcca tgataccaaa
    10741 accaaagaag gcattataga cgatgacccc gcctaaaact ccccaaagca atttacggtt
    10801 aaagacaact aaatgattca aactttctga aatacgaaac tggatcggcc ctgaggatac
    10861 aggcgcaact aaaatagtta acgctagata aagagccatg atgatgccat ttacaacgat
    10921 tacggataac cgttgtctgc ttttcgtttt ttgcattttc tctcctcttt tctcctagtt
    10981 tttttaacgc gggatggttg atgaaccgcg gttatgtgaa acaaacaata attataacag
    11041 acttttcatt tatttcaatc cgtcttatct gcttttaaaa aagcaaaagc ttaaacaatg
    11101 taaacgtttg ctaaaaatcg tgggatatgg tataaagtaa gtgtaagggg tcataagaat
    11161 tcccagtaaa gcagacgaag taggatctgc aagaacagaa ttgttgtgaa acttaaataa
    11221 ataaaaaagg atgtgggaat ttgagcaacc tcttactaga gaacggtcta gcaagcagct
    11281 gaagatctaa gtggacatgc cgaaccttgg aacttctcct tacgtaagt
//
LOCUS       DACDYI010000087        10767 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.087, whole genome shotgun sequence.
ACCESSION   DACDYI010000087 DACDYI010000000
VERSION     DACDYI010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.087"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            79..1125
                     /locus_tag="IWM52_002485"
     CDS             79..1125
                     /locus_tag="IWM52_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP9974702.1"
                     /translation="MTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPILSG
                     NEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQAPNY
                     NLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGISGN
                     KDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGPQMS
                     KTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSWGNY
                     VVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDSSLS
                     QSMLVDPKTLLFGD"
     gene            1474..2106
                     /locus_tag="IWM52_002486"
     CDS             1474..2106
                     /locus_tag="IWM52_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAP9974703.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            2694..4133
                     /locus_tag="IWM52_002487"
     CDS             2694..4133
                     /locus_tag="IWM52_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9974704.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4428..4646
                     /locus_tag="IWM52_002488"
                     /pseudo
     CDS             4428..4646
                     /locus_tag="IWM52_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4895..5110
                     /locus_tag="IWM52_002489"
     CDS             4895..5110
                     /locus_tag="IWM52_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974705.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5327..5583)
                     /locus_tag="IWM52_002490"
                     /pseudo
     CDS             complement(5327..5583)
                     /locus_tag="IWM52_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(5770..6681)
                     /locus_tag="IWM52_002491"
     CDS             complement(5770..6681)
                     /locus_tag="IWM52_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9974706.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(6700..7704)
                     /locus_tag="IWM52_002492"
     CDS             complement(6700..7704)
                     /locus_tag="IWM52_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP9974707.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(7701..9824)
                     /locus_tag="IWM52_002493"
     CDS             complement(7701..9824)
                     /locus_tag="IWM52_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9974708.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRENTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(9829..10749)
                     /locus_tag="IWM52_002494"
     CDS             complement(9829..10749)
                     /locus_tag="IWM52_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9974709.1"
                     /translation="MPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLY
                     GRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         3293 a         2032 c         2017 g         3425 t
ORIGIN      
        1 tcaagtgaaa gtagtacgat ttcaagtacc gaatcttcaa caacttcaag tagtacaagt
       61 gaaataactt cttcgaaagt gacaaaacca aaccatatag aatcttcaaa aaaggaagcg
      121 ccaaaaacgg aaggacaaac aaaaactttt tcgacaccac agcaaataga atcaacagaa
      181 tctttccaat cacaaccaat actatctggt aatgaagaaa ttcattttga caaaaatgaa
      241 tctgtagaaa attttattat gaaaattggt gagtctgcga gaaaaattgg tcagaaatat
      301 gatttatatg cttctgttat gattgcacaa gcgatcttag aatctgccag tggtcaaagc
      361 cagttagctc aggcaccgaa ttacaattta tttgggataa aaggaactta taatggaaac
      421 tttgtcatta tggtaacaaa cgaagatttg ggtaatggaa cgctttatac gacccaatca
      481 aaatttagag tgtatgaaaa ctatgaggaa agctttgaag attatgccaa attattaact
      541 aagggaatta gtggaaataa agatttttat gcaggagcat taaaagctaa tagtaaaaca
      601 tatcgagaag caactaaatt ccttacagga cggtatgcaa cagatacaca atactatttg
      661 aagctaaatg aattaattaa aacctacgat ttaaccaact atgataaaga agttaaaggt
      721 ccacaaatga gtaagactgg ataccaagtg cctattcata attattcaat aaccagtttg
      781 tttggaaatc gtggaaatga atttcaccga ggacttgata tggcagctac acagggtgag
      841 ccgattcatg cagttaaagc tggtaaggtt gttaaagcag agtttcacta ctcttgggga
      901 aattacgtgg tgatacaaca cgaagatgga tcaaccgcgt tatatgctca ccagcaacaa
      961 tacctagtaa aaactggaga taatgttttg caaggacaaa taattggcta tgtaggaagt
     1021 actgggaata gtactggccc acaccttcat ttggaaattt gcaaagatag tagtctttct
     1081 cagagcatgt tagttgatcc gaaaacctta ttattcggag actaaaaaag gtaatcaatc
     1141 tgacaaagta acaagaaaat ggttggattc ttagaccttt gatcaggata ttgagactgg
     1201 aattgatatc tcgattacga tgggtttgac aaataaaaca agtccattat cgaatatcga
     1261 gtgtattatt gccgtcttta tgctcatatt ttgagcaggt ttcactagat gaaaacaatt
     1321 tatctatctt gaggatttcg tgtacgtatc gttcagtctt gtattgtaaa ttagtcataa
     1381 aatcagacta agaaaaattg gaaatatttt tttctgattt attaaaagtt aattgaaatt
     1441 atatggtact tctttcttgg gagggtaggt gcagtgaagg aaagatatgg tcaccttact
     1501 gttgttgagg agattactca taatggatat aaaaaatatc gttgcctatg tgactgtggg
     1561 aatgaaacga ttgtatatcg atcaaactta ctatcaggta gaaccaaaag ttgtggatgt
     1621 ggaaattata aaaataggca taaatataaa aatttgacaa ataaaacttt tggtaagtta
     1681 gttgcaatca agccaactaa actgcgaagt gaaggaactg ttgtttggga atgtcaatgt
     1741 ctgtgtggga gaaaaaagtt agtatcggca aagcgacttc taaaaggtga tgtaaaaagc
     1801 tgtggatgtt tgaaatcaaa aaagcatcac attgaaaata aaaaattcgg attacttact
     1861 gcaaagtatc ctttggacaa taactgttca tggaaaacaa aatgggtttg tcattgtgag
     1921 tgtggcagta tgtgtgagat aagttatagt aacttatttt ttggacatac tcaaagttgt
     1981 ggatgtttaa aaaggcaaga aacaaggaca tgtgttgaag ggacagtaat agagtgtcta
     2041 acgtcaaaat taccgaaaaa taatacaagt ggtgttaagg gagtttatcg ttctaaagag
     2101 aaatagattg cgtatataac tttaaaaaaa aagaggtact atttaggatc gtatgattct
     2161 attaagagtg cagctcaagc tagaaaagat gctgaaaata aattgtttca acctttgatt
     2221 gacaaatatc gaggtaatca ttaattattg tggataataa ttaacagtga tatataaaca
     2281 tctataatat attattcata ctattgatat ttagtataat cctatggaat tgaatatcat
     2341 agcaatgtat ttgaaaagca aattggaaaa caaataggtg caacaccata aatatgaaaa
     2401 aattgggaaa aagtagaatg gaaatttgct tacgtctcac gagatagctc acactattaa
     2461 tctgaagaac aatgaagtcg acttttagaa attgctagaa gaaagtaaag cgaattatca
     2521 ctagttacta tagagtaagt tcaaataaag catctccaac ttacatggtt acatgaggat
     2581 atcaaatcga aattactcaa gcttcctatg gctagagtcg cgagaagttg ttgggcagta
     2641 gacataggtt atattgttct tagctacacg atcattagaa aggaaatcct attatgtata
     2701 gttatcaaga agatgcaaaa gtccaaaaaa atcgctggtg gattttgatt gctatatcta
     2761 tgtttacctt catgtccaca ttggatggca gtattgtcaa tattgcacta cctaccatct
     2821 cagatgatat gggcatacca atgaatcaag cggaatgggt agtgtccgtt tatctgatga
     2881 ttgtctgttc ttgtttgttg ctgtttggta aaattggcga tagccgtggg aaaatcaaag
     2941 tatttcggat tggtacgctg atctttgtct tgggatcgct gttgtgtggg ttcaatcatt
     3001 cgctaggtat tttattgttc gcacgggtgg tacaagccat tggtgctagt atgactatgg
     3061 ctacaaatgc cggaatcatc acggaagtct ttcccataca tgaacgtggt cgggcattgg
     3121 gagcaatagg tgcgtttgtt tcattaggct cgattgctgg accaggtctt ggcggcttga
     3181 ttttgtccca attttcttgg gcgtacattt tttggatcaa tgtaccggtg ggaatcgtaa
     3241 cgatgttgat tgctgaaaaa ttcttaccaa aagatatcac catgagcggc catacaatcg
     3301 atggaggggg ctttgctgcc attgcggcta cgattatcag cttttttgga gcaatctttt
     3361 tggggcaaga aagtggcttt ttccatccag tcccccttgt actgttcgta atttcagtga
     3421 ttgcctttgt cagcttttta aaagttgaaa agaaagcagc ctctccattg atttctttcg
     3481 gtatttttaa gaacaagatt tttaccatga gcttgattac agccttgttg attttttctt
     3541 ccaacttctt tgtcaatgta gtgattcctt tttatctgca aaatgctcgc gggttgagtc
     3601 ctagctatgc tgggctgttg atgatggtct ttcctttgat gatggtcgtc ggggcgcctt
     3661 taagcggcta cctgaccgac cgggttggcc cggagatttt ggtattagta ggcttgggct
     3721 tattgtcggt aacccaggtc atgtatatgt ttttgtcagt ggactctcct ttgtggctat
     3781 acattgtcgc tactgccatc atgggtgccg gcaatgcctt gttccagtcc cccaataata
     3841 cgatggttat gagctcggtc actatcgaaa acttgggact ggcaggctcg atgaattcct
     3901 ttgcccgaaa tttggggatg gtcagcggga tttcgctttc tacgacggtc ttatatcaag
     3961 cgatgtctgc taagatgggc cagcaagtaa ctaactatat cgcaaatcgg ccggatgtct
     4021 ttttatatgg aatgaagatt acctttttag gcagctttgt tttgtgtttt gttgcctttt
     4081 tcctgactat ctggcgtatc aaaaaacaag gaggtattca gaaaaatgtc tgaaaaaacc
     4141 actgttgaag aatttttgga ggcctgcgta tgggtgagtg ggatttccat ggatgatgga
     4201 agatgaagtg aacttctaaa agccgaaaaa ctcaagtatt atcttcaaaa tactagtgct
     4261 caatgagatg cttgatttga tcacaagaag actgcgaaag ccgctggcaa ccgcaagcta
     4321 ggtatcccac cgatggacta cgttttagag gctactggtt atgaatacgg agccaacgct
     4381 ctcatctgca taagaataca tcacccagat tatgtaaaga ttttcgatac ttctgtcaaa
     4441 aaatcattaa gatagataaa tgatttccgt ctagtcaaat tggttcagaa tgtagacaca
     4501 cagatggtaa gaagtcactc acaattcgag aatggacttg tcctatttgc cgtatacatc
     4561 ataatcgaaa cgtcaacgat agtatcaata ttttgaccaa gggctatgaa tacaagcatt
     4621 gccttagaaa acatcaagaa ccttaggaac aacggggata gcttggttaa taagagatac
     4681 cgctgtttag gcttgcaagt atactctttt atcaagaatt ccgtggcttt agtcatgaga
     4741 ggcttaatac tatgaaaatt ttctttagca tttttgattt aggttccttt tctaattata
     4801 ataagatgac cactacagta acctactgaa atatataaac agccactgta gtaccatttt
     4861 caaagtagtc tatgagagga gaatcttcat tcttatgaac gctggaattg cggatatgaa
     4921 tttaataaaa aaaaccctta atgattttac ttccaattca atatcaaaag gtactggtat
     4981 caatttaagc acaataaaaa agctgaaaag tggtgaaaga tcagtggaaa agctgaatct
     5041 tttagatgct ataaaaataa cggaatttgc tatgaaaaat ggaaaagctg agattgaaat
     5101 atggagataa ttatcgtcta gtgaattact tgccacttag ctgaatttaa cgatccttgg
     5161 tgcttgaagg gggagattct tgggaataga gcatacttgc aagcctatgt ctttactaac
     5221 aacgatatct cttattaacc aagctatccc cgtagttcta aggcttttac ttttatctaa
     5281 gcaataatcg tattcaaagg aaatttttga cccttagaaa gtctcatttt atcagacgtg
     5341 agcttaggca ttgctgaatt ttgttaattc ggttttaggt tgtgctttga gcataatatg
     5401 aaagtattct ttatcacgat ttcattcatc taaggtaatg tgatgtgatt ctgaaattct
     5461 ttcaaaggca gtttttgcat aagctgacat tacatcatca atgacatgtt tacaatgttt
     5521 cactactaaa ataagggggt aaccaagaag aaaaactgaa tgattatttg tatctaattc
     5581 catggtatat caaccatttc ctatatagac tgattatgac atggattaaa ataaaaaggc
     5641 actatgtctt aattgtcggt tttcgagtta ccgaatggca ttcatcaccc acctatagag
     5701 gatgggcgac ttctgcctga aataagttac actacttata acttctaata aaattagatt
     5761 gagtcattat tatttaacat caacgatttt ccagtttcca tttttttgta atactaattt
     5821 gtactgaaag tagctagtgg tttttgatac attatcaagg tattttatct ttgtactcac
     5881 tagaaaattt tttccgtttt gtagataaac tggattctct aactccgaaa actttaagtc
     5941 aatattgatt ggtcgcatcg caccattttc aacataatac tccaattctt tttcagaagc
     6001 tgatggataa agtttaaaaa atgtttctaa aaaatcggtg atttcttccg ttgttttggc
     6061 atcaatttct gaatcgtttt ctacttgttt tgcttcatat tttgccttgt ctggctttcc
     6121 aatcattgtt ggcaaactag ttactaaatg attcctgttt tcatcttcaa agattgtcac
     6181 tcgatatgca gaagaaattg attgcgtctt ttttccctct gtaatatgtt gctccactaa
     6241 aaatgaaacg acaaattctt ccgagtgttt ttcaacatct agaattttta cagattgaac
     6301 ttcggaactc gtgggaatat ctgctcgaac catatcttgg attaaagcga gaccgttttc
     6361 tgtaaggtac tgttcgagat tactcattct cttttcaatt gcttctttgt tatttttcca
     6421 tgaaaaatat tctttcgcaa aatcctctgt aaaactttca acgccagaag tattcactac
     6481 cttctctttt ataaccactt tttcatgagt tgtatgttga tcaatggcgg tgaaattttt
     6541 ataaacgcca aaagcaagac tacaagacaa taaaagccaa agggcaagca ccatttttcg
     6601 atgcttaccc acggatacaa ctcgttcttt tttctttttt gggctctttt ccttttgatt
     6661 ccgttcaatt tttatcttca ttctttttcc tcttttctct tatttgtatc ggccagctcc
     6721 gataagatgc tgccgccaat agttttccgt tagattcgtc caccccactg gatctcctgc
     6781 atgaaacatg cgattctccc ctgcgtaaat gcccacatgg gtcacataag taccggcatc
     6841 ataggttgaa tgaaagaaaa ctaaatcacc tggtttggca tcttttagat caatatgagt
     6901 catggcatca tattgatcct gagccacccg aggaagtttc aggtcaactt tggcataaca
     6961 ccattgggtc aagccactac aatcaaaccc cgtttctgga cttgagccac caaacacata
     7021 ggccgttcct tcatacttaa atgcctcatc aaaaatttct tggactattt tgtcatcaaa
     7081 ctgagttgta gttaggtatt gcttcactaa taaacagtaa aacatattgc cataattata
     7141 ccgccaccca ccattttcct taatcgcgat tgggttgggg taatcgactt tttgaccacc
     7201 tgatttttcc tttgaaaaag attcagctaa agcaaaacta tgcttcttcc cgtttttccc
     7261 gacatgatct agatacccgc caccatagtt ataggcctga ataatactat cttgatcaca
     7321 ccctagttct ttggaacgct taaccaaaga cgcaaaatag agacagcctt gtttgatgga
     7381 tgcctcggta cttaatgaat taggtggtaa tccggctgac tcagaacttt gcataacgtc
     7441 ttccattgtg ccaccagatt ccacttgaat aatcgccaaa agaattggaa tatactctgt
     7501 tatatccgat tctttggcat acttttccac cattgcacgg tgtttcaata cttcttctga
     7561 gacactagtt ccgccaggag aagtgccaga actaccattt ccctcactgt cttcaccaaa
     7621 caacaaaccc acacacatca aaatacccat aaaagagatg gtcagaaaag ttaatgaaat
     7681 aacacccact ttcttcaagt tcattttttc tcacctgacc ttgattttgg acgatggttc
     7741 attctttttt gtactttttg attggtctgc tttggcagag tggacctctg tttcacttgt
     7801 tggaaagaat ttttcttact tgctgattgg cttttccctt ttgaaatgat ttcttgacta
     7861 gttagttttc gtttgatttc gggattcaga tttgctctca caatccgctt tggtgcttct
     7921 tttttcagca tcatatcttt ctgaggttga gctgctttat ctattggaat attcgccctt
     7981 ctttttccct gacgatttgt tgcttcactc agtatcttgc gtttttcgtc cattttcgca
     8041 tttcttcgtt gtcgttgttt ttctcgcaaa tccactcgat ccgctttttc ttggaacagt
     8101 ccgcgcttga attcttctgg tgcttttttc gacttttcga ttcctttatg gagattgtat
     8161 ttcaaattcg ttggcgtatt ccttacctga tcctttactt gtttagtctt atctttcacc
     8221 aaagcttggg tatccaaaac tttgccgatt tttctcccag ccaaattcat tcgggattgt
     8281 ttcttattat ttgaaactgg attctcctta gttaagtcag atagtatttc gtgatcgttc
     8341 ggtaatcgtg aattctttcg tagagaagaa ccggatggct taaatttgga ttttgattgt
     8401 tcctttgcca acttatttcc gactgctgca ccaccaccaa aagccaaagc tcgtcccaca
     8461 tttcgattga gttgccgcaa ttttcgattc attaacattt gcggttttcg catgacgcgt
     8521 cttcccattg actggctatc attactctgg aggttgaaca tactcataat gtcgcccaat
     8581 ttgaaataga taccggcaaa ggtcacaatt tgcagaaaag caaccatgaa aaatggatag
     8641 gttgccgaaa tattgaaaaa catcgtggag atgctaaatg cagtcgtaat caccaaagta
     8701 acacccgctc gcatcataat cgtattgaat aaacgaataa tcgccttctt tcctaaactt
     8761 tcataagtcg gcaacatgga caataagaaa ctaattggca gaaacatcgc aaagataatg
     8821 aagagaattt gcgagaacaa cataatccct gttagaagaa aaacaaagaa ggaaatgcca
     8881 atattgaaca ataccaaaaa gaccacggtc cctaaacggt tcatggtctt ggtaatactg
     8941 agattcgcat tgtcattgtc ttcaatctct gccttgaccg cttcttcccg atctttgcct
     9001 ttgttttcat ctggactcac agatagaatc ttgttgaccc ggtcttcacc aatttcttct
     9061 atgttggaat catcaaattg cagtagtaac caaggttgtt ggacttgaat cgaaaacaaa
     9121 ctatcccgaa tcaaatccac actatctttt ccttggcttt ctgaattggg tacaacgatc
     9181 tcggttccaa gggtaagggc cgcttcacta acatctgagc taaagtcatt gatttttgtg
     9241 atataggtag gcgcataagc aatgaacgaa cccgatagta gaaaaatcat cacaaaatta
     9301 atcacggctc gaactgcttt cgtgttttct cgttttaata atccggtata cgccacatac
     9361 acccccagag ctaaaatcat caataataga aacccaaaat aaaatccgga ggtctggagt
     9421 ccattttcag taataccagc caatgtttgg atattctttc caatactttc agccgtatcc
     9481 gaaatgaaat ctaatttgta agcctcttga accacataac ccgtggcgtt cgacaaatac
     9541 aaactcaccg tccaaatgaa attagtaatg gcataaagac catacatcac gcttttccct
     9601 aagccatcac cccaattcca aggcaaccaa tcccaagagc tatccacaaa gaaatctagt
     9661 tgatagttgt tcagtgaata tttggaatat agatttcctg cttcaactgt atcatcaact
     9721 agccctgtcg cctcggctac cgtaccaact aaactcaata gaactatcag agtgagtatg
     9781 ctaataaaaa cgattcccac aatccgaaaa agtttctttt tcatcccctc actccattct
     9841 ctcttgaaca ggtggacggg tatcaaaggc atggaagagt tcttcaaaga ctggatgtac
     9901 ctgaaccacg cctacccgac catacaaatc ctgcatcaag cattggccat tttccaaatc
     9961 tcgtaggcgt ttttgattgc tttcatcttc tcgatccaag ccaaagaatt ccaaggtttt
    10021 cttgatttca tgaatatcgg tactgcgaaa cgcaaacttc aacccgatat tattcttcaa
    10081 gcgttcatcc agtaaatcat cactattttg cgtgacaaag taaaccccgg cattcatagc
    10141 ccgcccagca cgaacgagct tattcgataa tgttttccct tgcgctactt gaagaaaact
    10201 ccacgcctca tctaagtcta caattttgaa gatttctcga ttactatgga taaagtccaa
    10261 ggcaaaagtg gaaatgacga ttagcatgga aacactaagc aattccatgg tggtatattc
    10321 ttcaaaagtg gtttctgcat ctggtaaaac caaatccgct acttgaatga tatttaactg
    10381 cctatctaaa ctaatcgact gggtaatcga accatctgag aaaagcaaat gggcaaaatc
    10441 ataatcggta aaggactcga tgtgatccgc gatattttcc gcaactggtg agccttcttt
    10501 tcgaagctca ttaattactt gtaataatcc ccgttgattg ctttgggtaa ccgtccgaat
    10561 ggccttacgc aacactggaa acttctcccc atctcgacta gaaattcctg ttaaaaaggt
    10621 cagaatatca attgctagac tttcagaatc cttctttttc tccataatca cataaggatc
    10681 aagtaatcct tgattttttg gcttactagt taagttgaga atattgattt catccgcaat
    10741 ttctggcaag gtttctttcc agccacc
//
LOCUS       DACDYI010000088        10661 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.088, whole genome shotgun sequence.
ACCESSION   DACDYI010000088 DACDYI010000000
VERSION     DACDYI010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.088"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            333..2279
                     /locus_tag="IWM52_002495"
     CDS             333..2279
                     /locus_tag="IWM52_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP9974710.1"
                     /translation="MIERHVNLLKLLSRQEDFKPASFFSDKLAVSTKTIYQDIELLED
                     YVVNYQVTIERLPSLGIKLIGDRKNVEHLISSVENYLEKDIYSPEKRRVEIIKEVLFG
                     DKPVSLTHLSQSFMVSKTSLYNDLRIINKIIGSGEVKLQSSAEGISFTGNEQAIQRAI
                     KQLVFYYANDMESKTFFQVLNMLFDSKVISVIHDLLFNDYSELTEKVSDYYVRSLIST
                     LVIQCNRLLKGHHLTVEEEFLFNSIRYMETYIVANGLIEELKAKLNLPFYDADKEYLC
                     RQLFAHRVTNNLKTSNEEYAMSVHKLIERMSEIEKIDLTQNDRLYQSLLYHVPAMVLR
                     LKKGIKIQNPLLENIKSQYSELFSIVWYAMSILEEEFDVTLTDDEVSLILIHFQIAIE
                     DQSKANNIIIICQYGISSAQLIYNKVRRFLPAKDNVEISTLDKFHLAEIGEVDLIISS
                     LSFDAGGIPLVRVSPLVSKTDYMNIMEAYTKYLIEEPSRFIQNELIPKAVKVPTISKF
                     VQSSLVKTGVQVESKEECLDLMISELEANSLVTEEFRASIFSREKMGNTNLESGVALP
                     HANPSMVIESNIFIVALKNAINWGDRKVKLVIMVSLAEEDVSEIRGVYEELYQFIGQK
                     EMVDRLLQVHDSEKLLRIFQQKGE"
     gene            2282..2737
                     /locus_tag="IWM52_002496"
     CDS             2282..2737
                     /locus_tag="IWM52_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9974711.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            2751..4079
                     /locus_tag="IWM52_002497"
     CDS             2751..4079
                     /locus_tag="IWM52_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP9974712.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            4113..4397
                     /locus_tag="IWM52_002498"
     CDS             4113..4397
                     /locus_tag="IWM52_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9974713.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            4399..4914
                     /locus_tag="IWM52_002499"
     CDS             4399..4914
                     /locus_tag="IWM52_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HAP9974714.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            4930..5592
                     /gene="rpe"
                     /locus_tag="IWM52_002500"
     CDS             4930..5592
                     /gene="rpe"
                     /locus_tag="IWM52_002500"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP9974715.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            5599..6171
                     /locus_tag="IWM52_002501"
     CDS             5599..6171
                     /locus_tag="IWM52_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9974716.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(6358..7878)
                     /locus_tag="IWM52_002502"
     CDS             complement(6358..7878)
                     /locus_tag="IWM52_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP9974717.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
     gene            complement(8121..8306)
                     /locus_tag="IWM52_002503"
     CDS             complement(8121..8306)
                     /locus_tag="IWM52_002503"
                     /inference="COORDINATES: protein motif:HMM:NF017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HAP9974718.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            complement(8290..8472)
                     /locus_tag="IWM52_002504"
     CDS             complement(8290..8472)
                     /locus_tag="IWM52_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974719.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            8713..8931
                     /locus_tag="IWM52_002505"
                     /pseudo
     CDS             8713..8931
                     /locus_tag="IWM52_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            9426..10025
                     /locus_tag="IWM52_002506"
     CDS             9426..10025
                     /locus_tag="IWM52_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9974720.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            10089..>10661
                     /locus_tag="IWM52_002507"
     CDS             10089..>10661
                     /locus_tag="IWM52_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974721.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIGD"
BASE COUNT         3525 a         1674 c         1990 g         3472 t
ORIGIN      
        1 taggactcaa ttgttcatag atgagcattt gtaattaagt tatattataa taaaaaaata
       61 cgaattttag tgacgccatt atattgtttt tatttttcaa ttgtaatatt tcatattact
      121 tccataataa ggagtatttt ctattggaat atcttagctc ttttaaggat ttctcctttt
      181 ttgtttctca ttttttaaga ttatttggat ttcctttaca acaaacttcc acaagtatta
      241 ttgacataga tgtaattgag ggaagggggt tccagtgcta gaataaataa tgaaaagagg
      301 taacaaatgc tagcgttaag aggtgaacaa gtttgattga aagacatgta aatttattaa
      361 aattattaag tcgacaagaa gactttaaac ccgcttcttt ttttagtgat aaattggcag
      421 tgtcgaccaa gaccatttat caagatattg aattgttgga agattatgta gttaattacc
      481 aagtaacaat tgagcgatta ccttctttgg gtatcaaact cataggtgat aggaaaaacg
      541 ttgaacattt gatttcatcg gtagaaaatt atcttgaaaa agatatttat tctccagaaa
      601 aaagacgtgt cgagatcatt aaagaagttt tgtttggtga taagcctgtt tctttaactc
      661 atttgtctca aagttttatg gttagtaaaa catcgttata caatgacctg agaattatca
      721 ataaaattat tggcagtgga gaagttaagt tgcagtcatc tgcagaagga attagtttta
      781 ctggtaatga acaagcaatt caacgagcaa tcaagcaact tgtattctat tatgcgaatg
      841 acatggaatc aaaaacattt tttcaagttt taaatatgtt gtttgattca aaagttatta
      901 gtgtcattca tgatttgcta ttcaatgatt acagtgaact gactgaaaag gtttctgatt
      961 actatgttcg gtcattgatt tctactttag ttatccaatg taaccgttta ctgaaaggtc
     1021 atcatttaac tgttgaagaa gaattcctct tcaatagcat tcgctatatg gaaacgtaca
     1081 ttgtggcaaa tggtttaatt gaagaattga aggcaaaatt gaatcttcca ttttatgacg
     1141 cagataaaga atatctatgt cgtcaactat ttgcacatcg tgtgacaaat aatttaaaaa
     1201 ccagcaatga agagtacgct atgagcgttc ataaattgat tgaaagaatg agtgagattg
     1261 aaaaaattga tttgactcaa aatgatcgat tatatcaatc cttattgtat catgtgccag
     1321 caatggtcct tcgtttaaaa aaaggtatta aaattcaaaa tccgttatta gaaaatatca
     1381 aatctcaata ttcagaactc tttagcatag tatggtacgc catgtccatc cttgaagaag
     1441 aatttgatgt gactttaacg gacgatgaag tttccttaat tttgattcat tttcagattg
     1501 ctattgaaga tcagtccaaa gcaaataata tcatcattat ttgccaatac ggtatttctt
     1561 ctgcgcaatt aatttacaat aaagttcgac ggtttttacc agcaaaagat aatgtagaaa
     1621 tatcaacttt agataagttt catttagctg aaattgggga agttgatttg attatttcct
     1681 ctctttcctt tgatgcaggt ggtataccct tagtaagggt atctccattg gtaagtaaaa
     1741 cagattatat gaatattatg gaagcttata ctaaatattt aatagaagaa ccttcgcgat
     1801 ttattcaaaa tgaactgatt cctaaagcgg ttaaagttcc caccatatct aaatttgttc
     1861 aatcatcttt agtaaaaaca ggagtacagg tggaaagcaa agaagaatgt ttggatttga
     1921 tgatttccga actagaagcc aattcattag ttactgaaga gtttcgtgct tctatcttct
     1981 ctagagagaa aatggggaat actaatttag aaagtggtgt tgctttaccg catgcgaatc
     2041 catcaatggt cattgaatcc aacattttta ttgtagcatt aaaaaatgcg attaattggg
     2101 gcgatcgaaa agtcaaatta gtcattatgg ttagtttagc tgaagaagat gtatctgaga
     2161 ttagaggagt atatgaagaa ctttaccaat ttattgggca aaaagaaatg gtcgatcgct
     2221 tgttacaagt tcatgatagc gaaaaattac taagaatttt tcaacaaaaa ggagaataaa
     2281 aatgtatcta gataaagaat tatgtttatt tcaattagaa gtgaaagatc aaaaagaatt
     2341 gtttcaagta atgtctgaac aattaaaaaa cgctggttgc gtgaaagata gtttcttaga
     2401 aggtatcacc aaccgtgaac aagaatttcc aactggatta gaagtaaatc aaattgggtt
     2461 tgctatccca catacagatt ccgcacatgt aaatagctct caaatctgtt ttgcctcatt
     2521 aaaggaacca ctggtatttt cagatatgac agataaatct catgaaatac ctgtacgttt
     2581 agtctttatg ttggcaatgt cacaacctca tgaacaaatt gatacactac aaaacttagt
     2641 tagcctattt caaaatgaag aaaaagtaaa tgaattactt gcatgtacca cgaaagaagc
     2701 atttattgag ttagtacatt ctgcaggagt ttattaggag gaatagagag atggatattg
     2761 tattaaatgc ctttgaatgg tttattggat tagggtcaaa cgtcttttta ccactgatta
     2821 tttttgtaat cgggatatta tttggattaa aacctggtaa agcatttgtt tccggcgtta
     2881 ctgttggtat cggttctatt ggtttaggat tagttttaag tttattatca gatggtttag
     2941 gaacagcaat tcaacaaatg ggggaacaat acggtggtgg cctgaatatc ctagatattg
     3001 gcgtaggtgt tggtggacct ttggcttact ctacatcatt gggaatttta atgattccaa
     3061 tttcattaat tcttaacttc atatttgtgg ttttgggttg gacaaaaaca ttgaatgttg
     3121 atatttggaa cttttggttc gcaattttcc taggtatgat tactcaatct gttacgggaa
     3181 acttttggtt tggcttaatt ggcgcgattg tcgcagtaat tttacaatgg ttcttagcag
     3241 atcgtacaca aaaaactgtt agcgaatttt tcggctatcc aggaatctct atcacacata
     3301 tgatgggatt atcaggagta ctatttgcca aaccagtaaa ttgggttttt gatcgtattc
     3361 ctggatttaa taaaatagat gcagatgcag aaacattaac aaaacgtttc ggtgttttag
     3421 gtgacacagt tgttattggc ctaattatcg gtattgtagt gggtattatg gcaggctatg
     3481 acgtagcagg aattggaaac ttaggaatta tgactgcggc agtgatgaag attatgccta
     3541 aaatggtagc aatgtttatg gaaggtttaa tgccaattgc agaagcggcg aaagaattta
     3601 cgaataaaaa gttaaatggt cgccaagtaa acattggaat ggatgcagcg ttaactgtgg
     3661 gtcatccaac tattatgtca acttctctat tgcttgtacc aatctcatta ttgctagcac
     3721 taattcttcc aggaaataaa gtattacctt ttggagattt agcattcttt gctttcgcca
     3781 tctgtttaat gattccttat tttaaaggaa atattgtgag atcattagta ggatgttcgt
     3841 tgtatgttgt tacaacatta tatatggcaa catggttggc accaattatc actgatgtgt
     3901 ttacattagc aagtttcgat gtggggacag caggattagt aactatgttg ttatgtggat
     3961 tatggccagt agggttgttt gttttattag catcttactt tggtgtaatt ggaataggtg
     4021 gattaggagt tgttttagta ggtgcttcta tctacatgaa caaagtgaag aaagcttaat
     4081 cataaaatta aaataagaaa gaagagatca atatgaaaaa attattagtt atttgtggag
     4141 caggtcatgc tacttctaca attgcagtag caaaattatc aaaatggttg gaagaagaag
     4201 gattaacaga tcaagtgaaa atttatcaat ctaaaattgc tgatgagcta aataaaattg
     4261 atgaatatga tgctgtagta agtacaacaa ttgtccctga tgtaattaaa gctaaagtaa
     4321 cgaatggctt gggcttatta acaggtattg gtacagatgc catttatgca gatgtaaaag
     4381 aaagattagg tgtttaatat gcgttttcat catgacactt tgactattga aacagtttca
     4441 gggcgtcctt cgtatcatga tattcgaaaa gaacttttag atacagttga tacagcaggt
     4501 attgagaatg gaatgttagt aatttcgagc ccgcatacaa cttgctcact cttttttgaa
     4561 gaaacgatgc atgacacaaa cttttttggt gatgattatt tacatgtaga catcaacaat
     4621 gtaatggaaa cgattattcc aaaaatgacg agtgaaaatc aatacaacag tccggggcca
     4681 aaacacattg attttggaac aagattatct gatcccaatt accctgcaga aaaatgggtc
     4741 atgctaaata ccgatgcaca tttacgttca tctatttatg gaaccaactc aatgacttta
     4801 attgttcaag aaggtaaact tttattagga gaaatgggcc gtgtctattt tgtcgattgg
     4861 gatcaactaa gagagcgaac tagaacaatc aatctcatga ttatgggaga ataaacgggg
     4921 gaatagagaa tgacaattgc tccatcattg ttaaatgcac ctatcgcaga agttaaagag
     4981 ttatttgaaa tcttaaaaga aaagaatgtt gattatttgc atattgatgt aatggatggc
     5041 tgttttgtac cagatatggc ttttggacca agtgttatta aagatattcg tccactaaca
     5101 gacgttgtgt ttgatgttca tttgatgatt gatacaccag aaaaaattgt cacaagattt
     5161 gtagaagcag gagcagatat tgttaccttc cacattgaag ctaccaaaca cgcaatgaag
     5221 gtcattcagt tagtcaagga agcaggagca aaagtaggga ttacttttaa tcctgcaaca
     5281 ccagtcagtt ttattgaacc agtattatct ttagtcgatc aagtattagt aatgacaatc
     5341 aatccaggaa cagagaataa gcattttatt caagagacag tagttaaaat cgaacagtta
     5401 gatgtaatac gcaaacaaaa tgattatacg tatgatattg aagtagatgg caagattgat
     5461 aatcaaacga ttaaagtttg ttctaaagca ggggcggata tttttgtatc aggcggtttc
     5521 atcttcaatg ggaaaaatac cccgcaagaa caaattgata aactaaatca atcattagag
     5581 gaagtgaagt aaaagatcat gaccaataaa gaccaaaata aaaactattt agaagcgatt
     5641 atcttagaat tacaaaataa ctcaaaacgt attaattctg tagagttaga acaatttagt
     5701 gagaaaattg ttcaagctaa acacatcttt ctagctggtg caggacgctc tggaattgcc
     5761 atgcaagcat ttacgaatcg cttaatgcat ttaggttttt cagtcagcct tgtcggtgaa
     5821 attagttcac cacattcgca acctggagat ttattaatta tctgttctgg ctcaggtgaa
     5881 acaggtagtt taaaaaattt agcggccaaa gcaaatcaaa gtggtgtaag cattgcttta
     5941 gttaccatga aaaaagactc gtcgattggt caattagcag attctgttct tgtattacca
     6001 ggtattacca aagatgaaaa tcaacgagaa gaagatactt tttcacaacc tatgggttca
     6061 agttttgaac aattagcatt tctcaccttt gatagtttag tgttatcttt aatggataaa
     6121 acaggtgaaa gctcagaaac gatgtttgct cgacatgcag attttgagta gtagatttta
     6181 aagataaaaa accattctct aaatgctaat tattcagcaa atatgagaat ggttttttta
     6241 tttgattaga cataacttaa taattcagta gttttgagcg taagcgtgtc atagaatggt
     6301 acctaaacac aaatagcccg atttgataca ctcaaatcag gttatttgtg gcttatatta
     6361 tttacagatt tcttgtatct gctgattcca tggcaaataa gcgcgtagcg cttcaggtgt
     6421 catcgaagac agattgggaa tctctgtaaa aagatgattt aaatatttgc ggatgtttaa
     6481 cccattcgca acagctgtct ggataatact caatataatt ccatttgatc tagcgccttt
     6541 taagctttta gaaaatagcc agttttttcg accgatcact aactctttga tggcacgctc
     6601 tgctcggtta tttgaaagtt ccaagcgtcc atcaagcaac acaatctgaa gtccgtcttt
     6661 atgattgact gcgtattgaa ccgcctttcc aagctgcgat tttggcaaat aagcaaaact
     6721 ctcacaccaa tgaaaaaatt cagtcatcac aggctgcaac gtttccttcc tcgctacaaa
     6781 cctttcttct gcgctaagct tattaaaggt ccgttcgatt cgaaacatct tgtcacagaa
     6841 gtctaatcct attttggcat gactttttgg ggacggttgt ttacccaatg cttcaaaaaa
     6901 ctttctgcga acatgcgccc aacaataaac attgtttacc ggcagctttc cataggccgc
     6961 gtaaccatca gtatgcaaga aaccgctaaa tgattctaaa aaactttttg gctcatcaac
     7021 ggaacgcgaa tttgcgtgct cgtagagcac aacttgtttt tcttcgcttt tacctgaagc
     7081 atataaccag taaaaggtct tttctttatg agtattcaat acccgatagc ttgtctcatc
     7141 cgcgtgtaag acctcctgat tgcttaattc ttgatgaagg caattgtaga ttggctctaa
     7201 aaccatttca cttaccttga tatgccaatt agtgattttt ttacgatcaa gagggaaacc
     7261 aagtgcatgc cattcttttt cttgtcgata tgccggcacc tttttaacaa atttttccca
     7321 aatactctgt gtcacaattg aagaggaacc tagactatta ttgatcaacg gtttaggaac
     7381 aggcgctttt ttgatttttg tttctcctaa tgcttcacat tctctgcaat gataggcatg
     7441 ttggtagatg acttgttttt ctatatgggc aggaacatac aatggctctt ctctaagcct
     7501 tgtcgcaccg atatcatgta aagtatgacc acagtcttca cacaaacaat cggcaccttt
     7561 taattcataa tgaataggaa cttcaggcag accttctaat gaaagtgctt tgagtccctt
     7621 gcgttttctg cgtttaaaag aagataccgt aatgggttcc tggctttctt gtccagtgtg
     7681 ctctggttca ttaaaagttg agtcgtctaa taaatcgatt tgcccatcta cgggtggtgt
     7741 ttcttttttt cgtccaaaca agcgtcgatt catttcagca atttgttctc taagaagagt
     7801 attttcctgt tgctggattt caagcaactg tgttaattgc ttgatttgta caagaagttc
     7861 agattccatt tttgtcattt ttacaccacc attctagcga ttttcttaca tctattatac
     7921 cattttatga caacgaaaaa cagacaaaat ccttgattta acaaggtttt cgtctaatat
     7981 atcgcacctt ttttcgtttc ttttagcgta catttttctt cgattgaaag gccagtcagt
     8041 aagacttctt ttctggaacg cggccattga aggtacccac tttcaaatct tttgtacatt
     8101 aatacaaatc cattttgatc ctagtaaaga gccttgaagc gatctttctt cgtaccgcaa
     8161 aataagaaaa gcgcttgatc gtaaacatca aggtcatatt cttctataac gaaagctgct
     8221 agtccatcaa ttccttttcg aagatctgtt tttccacaaa cgataaaaat atgtttcaat
     8281 gcagttgcat caatgagcat tggcattcaa ctcttttaac aaggtataca aaagatattt
     8341 atccattcca ttataaataa atatttcctt atcttccgat ttgattctgc aagctagttt
     8401 tggcttggtg tcaatttgcg atttttgagc cgcacttgaa aattgaactg gtacaatatc
     8461 ttttttattc attcgttttt ctccttctat atttgatagt ttaattatag aagaaagata
     8521 atcggcgtac gagatactat cctatgacac gcttacattt aatttaatat tttgcctcaa
     8581 attagccaca ctttttatag aactaagagg aaaaatgaag ttttcttcct attaatatag
     8641 ttgtttgaaa tcttaaatat agcgttttac gatgtaatgg gaagtatggt gaataaatca
     8701 actatgttcg tacgaagcat ttactcaact aatttaatcg aatctttcaa taaacaaatc
     8761 aagaaataca gccaccgcaa ggaacagttc caaaatgaag agtcgatgga acgtttctta
     8821 gtctcgtctt ttgatactta caaccaaaaa ttcctaggtc gcagtcataa aggtttccaa
     8881 caggccgaag gcgagctcga acaaatgctg agtcaaccga tggagaatta gaaacaatct
     8941 acagtaggga agaaccattg acacaaaatt attgacgctc ccaactaaaa gtgtcaaaat
     9001 aatgatgaaa tcatttagtt cttataaatt agaaaagaga aaataaatat tccatatcta
     9061 ctttattgga tccgtaacat gactaaattc tgaatacttt ttaaagctta tcatttggta
     9121 aaactgaata taagttaatt atatagactg aaaattttga attttcagaa tagaataact
     9181 taaaagtact gacaaaattt tttaaagggg caatattaat gttctgagat aatcaaatcc
     9241 taccgaaaat atcaaaaatc caatgtgaca caatcagatt gtgtctcatt cgagggaatc
     9301 acacaatccc acgtggctct aggcagaatt gaatttttgc ttggggcttt ttccttatat
     9361 tgattttgaa tgagacttat tatatgttat aatgcaatta agtatcattg gagagggcga
     9421 agaaaatgaa gacggtagaa cctattcgag acaaaaaaaa gatcgatgcg atgaaggcaa
     9481 tcttggcttc gggaaaatat ggccaacgaa atttggttct tttttcgatt gggatcaaca
     9541 ccgcctatcg catttctgat ttgcgtcaat tgaagctggc cgacgtgctg gaaatttcac
     9601 gcggtcgcgt ggtggtcaaa gatcgattag cgatgaagga acaaaaaaca gctaaacata
     9661 attcggtcta tatttccaac aaattgcgaa aaacgatttt ggagtacgtt caacaggaat
     9721 ttcctgatca actccttcag caagattttg acggttattt gttccctagt cgaaaaggga
     9781 cgaatgaacc gctcaaccgc caaagtctgt ggcgaatcat tcatgatgct ggcacggcaa
     9841 tcggattgaa agacattggc ccacattcca tgcgaaaaac gttcggttac tttctctata
     9901 aacaggggac caaaacagaa atcattcagt ccttgttgaa ccattcttct cagcgcgaaa
     9961 cattgcggta tattggtatt acacaagaag acaaagatac agctgtaaaa agcttagatt
    10021 tataatataa aagttaagtt taaacatgga tagttttaaa ttagtagatt gagtgttggg
    10081 aggaaagaat gagtacatta tatattatag gaaacggttt tgatttaagt catggtttaa
    10141 aaactagata cactgatttt tcaaaatggg ttgaaaaaag tcatagtgaa atattcgata
    10201 aaatagacac aatgataaat gaaaataatg aggtgagaag tacagctgat gataagatag
    10261 gttggtttaa ttttgaaagt agtttgcatg agttaattca aagtccagtc tttgaagatt
    10321 atgtagaaga gtctaaggat tatctcatag gttatggaca tgaagattgg tcagattctg
    10381 cccatcatga ttttcaaaat atggtttatg attatgttgt ttctctagaa gaattacaac
    10441 aacttttcaa ggaatggata cgcaaagaaa tacagccgac aatagaacat aaaattccaa
    10501 aataccaatt ttcgtccgat gtactattct ttacattcaa ttatacagat gttttggaaa
    10561 ttctatacaa gcaaccgaaa gaaaacatat atcatattca tggcagttat gacgatataa
    10621 taatcggcca ctccgagaat tttgacaact ttataggaga t
//
LOCUS       DACDYI010000089        10595 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.089, whole genome shotgun sequence.
ACCESSION   DACDYI010000089 DACDYI010000000
VERSION     DACDYI010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.089"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(1273..1938)
                     /locus_tag="IWM52_002508"
     CDS             complement(1273..1938)
                     /locus_tag="IWM52_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9974722.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2064..4100
                     /locus_tag="IWM52_002509"
     CDS             2064..4100
                     /locus_tag="IWM52_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAP9974723.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4104..4742
                     /locus_tag="IWM52_002510"
     CDS             4104..4742
                     /locus_tag="IWM52_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9974724.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4770..5198
                     /locus_tag="IWM52_002511"
     CDS             4770..5198
                     /locus_tag="IWM52_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974725.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5655..6566
                     /locus_tag="IWM52_002512"
     CDS             5655..6566
                     /locus_tag="IWM52_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9974726.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            7020..7334
                     /locus_tag="IWM52_002513"
     CDS             7020..7334
                     /locus_tag="IWM52_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9974727.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            7350..7736
                     /locus_tag="IWM52_002514"
     CDS             7350..7736
                     /locus_tag="IWM52_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9974728.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            7765..9150
                     /locus_tag="IWM52_002515"
     CDS             7765..9150
                     /locus_tag="IWM52_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP9974729.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            9153..9305
                     /locus_tag="IWM52_002516"
     CDS             9153..9305
                     /locus_tag="IWM52_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9974730.1"
                     /translation="MLAGVAIATPALNPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQ
                     RMSNGL"
     gene            9328..10533
                     /locus_tag="IWM52_002517"
     CDS             9328..10533
                     /locus_tag="IWM52_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9974731.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
BASE COUNT         3596 a         1725 c         2061 g         3213 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgaggacta cctgtattag tcctcttttt
       61 tgaccagatt ttgaccagtc tattttacat tctaatatat tcatatgagc taattttatg
      121 attaaatggt aagtgtttcc ttgtttgttt tagtttaaac tagtgtgtaa tttaaaaatc
      181 tatatggaag ggtctgtggg gtaatactta aataaaggct aggaacctga taacatcaag
      241 gttcctagcc tttttataat aaaatgaagc gaaaataatt tcttttaaag agccgagatt
      301 atcagctata taagagtgaa tttgatgcaa aaaacagagc cgaaacagtc ttggtgttcg
      361 tagctctgct tatatgtttc gaatttaaga caaagagttg gcaaaagatg ttgataagga
      421 agataagata actgtgttat ataaactagg taactaactg taacaagcta attttaagag
      481 attcttatta gaagacgttt aggaatataa ctaagaaaat gattatacag gtaattgtca
      541 tttcaataat gttttctaat tttcgcgtct ttgttttaag aaaaaggcca gaatcagttg
      601 gaaaaaagtt cgtacgatag tgaagagggg caaaatttcc tactaatatt cccactaaca
      661 agagataaaa cggccaggtt ggtgtgattc ctctataaaa aaatgcccat gctaccgaaa
      721 tcccatagat taacaactga acaattttta caatgaaaaa attgttctgc cattactcaa
      781 agtctcttcc tccttgtatc gtttatttgt ttcattttat cattatgata ggcgcttgaa
      841 caatccattt tgtattttat tgagtactct tggataaact accagcgatt atcatattta
      901 tttatgttac tgtaagccat catataaaag taaatgagca gatcatagaa atataaaaaa
      961 gaactaaaat tgatctaaat tatgtgcagc ttgactacgt accaatctat attacttact
     1021 agattggtcg ttaatctatt cggatcgaat gagcaaaata tttttgtgaa tgtccatcct
     1081 tgattatgat tccaaaaaga atcattggga ggctgtggcg taaatattat actataagcc
     1141 agaaatacct atcattctga acttttttat ttaggaactt tcatctcaag gctttttcaa
     1201 ttaatttgta cccctgtttt ttggataaga tcatgtgcat tgattttttc tatctctacg
     1261 ttcatctcct acttaatgcc tatctggctc ctgagggctt ctttttaagg cgtcgtcata
     1321 gtcttggctg aggatttctt ggtaatctga gggaatggct tcccaagtga gcaggcgaac
     1381 gttcatgacg tgttttcctt tgtgtttgat tacagcatgg tttttctctt tttctacctc
     1441 tccagcgtga aaagacagcc atgtaccatg gccaaagata tcattacata cccaacgttc
     1501 gatggaattg tccacgtatc ctacagggag tgtggtatag ccgatttgtg gtttcaaaaa
     1561 aggattgaat cgatcgatcg tttcattttt gtattgataa ttgataagaa aaagatagac
     1621 caaacaaaag acgagcagaa agaaagatag tgtctttttg agtgagaagg gttgttttga
     1681 tgaataagat ttcttgttaa caagaggttc atctggcaat gttggttcta ttgattcggt
     1741 agctgattca atagaaggaa aatcaatcgg cagacctaaa gtatgatgta aaagtgacag
     1801 catttcaagg tctggcattg tacgagaatt ttcccaacta gaaatcgttt tatcggaaac
     1861 accaattttt tcgcctaatt ctttttgcgt aagcttacaa tttctgcggt aagttcgtat
     1921 tttttctccg gtgttcatct tcttctcctt tcgattctac agtatataga tttattctac
     1981 atgacctata cacttgatag atacgttttt ttgatgaaaa acggtatgat tttatcaaag
     2041 aatcatttaa gtaagggaga gtggtgataa tcaaaaaaaa gttcacagtt ttactcacaa
     2101 tcatagtgac cggtatcatg agtggttttg ccttggttag tataaataaa caggtactct
     2161 atcggacaaa tgaatttttc catcaaacag atacttatac tccgattcga ttgccacaac
     2221 attttattgg gcaagaagaa tatgagcagc taatcatggt atttgaaaat gtctcatcag
     2281 aaactgagct ttcttatatg aaaaaaacag taaatgaatc caatgaagta gatacatctt
     2341 ggaacttcaa gatacagcca aagcttgaaa tgacattgta ttatcaaggg gatgtcaaat
     2401 cttctcacga gaaggtaccc tattctaata cctcctttcg cgtaacgtcc attgaagaat
     2461 cgatcaaaat aaaagatttt gaaggtgaat ttttcttgat gacgagagaa aaagaagcct
     2521 atgaacggtt catttcacta ttcactgatc gattcaatca agcatttcac actgattatg
     2581 ccactaatat ttttgaagac tttgaaaata gctattcgta ttcggttatt tcaaatacag
     2641 acgtatataa ttatcgagtg tatttaacta ttggattgat tcttttattc cttttgttat
     2701 catccttttt cttattgatg aataaagaaa tcagtttatt gttgaataat gacttttcat
     2761 tacatcaagt attgtggtgt ttattgggga aaaagaaaat cattattaca ttcataattg
     2821 caggaatcat tggtggtttt atttttatgt attataagga gcaatttctt tatttgttcc
     2881 agatgtacgt tttcttgttc agcggaatat atggtatctc tttgatttct cttttattag
     2941 ttgaaaaaat cgcttcgtta agagaagcgt tcaactatat ccaatacatt ctttttacat
     3001 ttggattgtt gttttctctt cttttgaccg ttgccaatgt cgaattagca actatcgttt
     3061 tttcttcttc acagttagct gtttcccatg aaccaccaaa agaaatcgct ggtgaggact
     3121 atcatgtatt ttatccagta accatcggga aaaaccacgt ggattttatt tattccaatc
     3181 gttttgcatc agcagcagat caggaattat atgaaacttt gaataaaaac ggaagtatat
     3241 tagtcaatgt atcagattac ttgaatgaag aaaatgaaca gtttggcaga gggataaaaa
     3301 taaatctgaa ttatcttaag aaatttcctt tgattgatgt ctctggaagg ttgatccaaa
     3361 taaccgaaaa agaaacgcac cctgttatat tgatccctga acgatataga catacagatt
     3421 taaaaaatga cctggcgcaa ataacagaat attatcaaga aatcagtgaa aaagaaccga
     3481 aatttctatt tataaaaaac aatcaaccca tttatacatt tattccttct atcccctgga
     3541 tcgaacatta tccggttgtt gaaatactga cattgaaaaa cagtatgtat ttggaaagaa
     3601 atattctcag cggagaaatc tatccaccgt taaagatcaa gataggctct ttgtcgaaag
     3661 aaaggttatt tgagctgatt gaagaaagtc agttacgtga taacttgcag ttatcatttc
     3721 cgtatactca gactgaagag atagctgtca aagtgttaag cagatcattt cattacttga
     3781 tacagatatt tttactcact ttcttttctt tcttcttgtt gagctatgtc atgttatgca
     3841 tatattttgt ctacaattgt cggaaaatcg ctatctttcg ttcaagtggt tattcgctct
     3901 ttgaaacgta taaagatttc ttcatgatga acctcatcaa atggggaacg acaagtgtaa
     3961 tcttcctttt tcttatagaa agagagccta aatatctttt taatattttt ttctttagct
     4021 tcattgattt tattctttca gttgttttta ttttgtctac agaaaagaaa agtcagttat
     4081 tgctgatgaa tggaggttaa caattggagc cgttgatagt gataaaaggt gcgaaaaaaa
     4141 tcattcgaaa caaaacgatt cttcatttgg atcgtttcac tgcggatgct ggggatcttg
     4201 tgactgttgt tggagaaagt ggagcaggta aaagtacgtt attgaatata ttaggattga
     4261 atgatcagtt cagttcagga caattctatt tgttcgatca agagacgaaa caattaactg
     4321 ggagagagaa gcgcttgata aaaagaaata ccatatcttt tctttttcaa gattttgggt
     4381 tgattgaata tgaaacaatc gattttaatt tagatattgg attgaagttt tcgaatttaa
     4441 atgtttcaga taagcgaaca gcaaagtatg aggcattgaa aaaagtaaat ataaacaaga
     4501 agctaagtac gcctatctac acattatctg gtggtgagaa gcaaagaatc gcctgtgcga
     4561 gagtcttatt gaagaatagc aaaatcattt tagccgatga accgactggg tcattggata
     4621 aaagaaataa ggataaagta gttgaaatct tatcgcaaca agcttcccaa ggtacgttag
     4681 ttatcgtagt cacgcatgat ccatatctga ccaaaatagg aaacaagaag atcatgcttt
     4741 aaaaatcaaa aaggaaagga tgtcgtttta tgaaaaaagc agttattatt tgtgcactag
     4801 ggataggttt tgttggagga aacgctatgt catctgaagt ggaagcaaat tcagttcacg
     4861 gtgaaatcta ttacaaatct ttaaagccag gagagattca ttttgatgct aaaggtaata
     4921 ttattaagtc aaatttacca gtagaccggg aaatttctag aacagcaatt aatcatagct
     4981 ctggaagatg ggtttattat agtgatacaa cttgtacgag aaaaacaggt cattctaacc
     5041 actatagtcg tgtctataat cgtcacggtg caaaagcgaa agttggaaac tctattcgat
     5101 cagcaaatgc aacaaaagga gtatgggcct atgcaagtgc gacaggaaat agaactgaca
     5161 cattctcatg ttggtacaat ccaaccggtt ggtattaagt atccaaaagt ttatataaaa
     5221 tagaaaaaac atataatgaa acagcgttaa aagttatttt tggtagtttt aatatattca
     5281 tttttagttg tataaaaata tatcataata aaaacaacaa aaaaatgtct ggaaaatcga
     5341 acataaaagt gtctgatttg aatatcccag cagcggaaga agaaaaacaa aatcgataac
     5401 atttgttcta agaattagtt acgttaaata cggaacgcaa gatagacttg aaaaacggta
     5461 acaaaagaag tagtatatta aggtgtgatg tgctacttga catttattct gctgagcgaa
     5521 ctttctggat tgtgtagcct tatatctatt caggacgtat cgagcagatt agtaaaggtg
     5581 ttcttgatga ttttcatctt gaacaccttt gtttttagca attcgcaaaa aacgaaaagt
     5641 aggagaattg ataaatgaaa aaaagatttt tagcacttgc catcgtacta ggcactggct
     5701 tattgtcagg ctgtacgaat gccggtgaaa aaacagccgt tagttataaa ggcggtacaa
     5761 tcagcgaaca agaagtaatg gatagcttga aaaaaatgca aggagcagat tccgctgtgc
     5821 aacagttgat cgtttatcaa gtatttgaag ataaatatgg agatgacgta tcaaccaaag
     5881 aaatcgattc tcaatatgat caaacaaaga aacaattggg tgattcattt gattcacaat
     5941 tgaaatctgc aggttacaca gaacaaacat tcaaagacag tatcaaacaa agtctagcct
     6001 ttcaagaagg tctaaagaaa catatcaaat tgaccgatga agatttaaaa acggcttggg
     6061 aatctttcca tccagaagtg gaagctcaaa ttatccaagt agcaagtgaa gatgatgcaa
     6121 aagatgtgaa gaaagctgct gataagggag acgatttctc taaattagcc aaagacaagt
     6181 caactgatac tacgacaaaa gaagatggcg gaaaagtgaa atttgattcc actactacaa
     6241 cggttccggc agaagtcaaa gaagcagcct ttaagttgaa agatggtcaa gtttctgatg
     6301 tgatcacttc taccaatgct tcaacttata ctacggaata ttatgtggtt aaaatggtga
     6361 aaaaccaaaa caaaggcaac gacatggata aatacaaaaa agaattgaaa gaaattgcaa
     6421 cagacacaaa attaagtgac agtaccttcc agaataaagt aattggcgaa gtattgaaag
     6481 acgctaatgt aaaaatcaaa gacaaagact ttgaaaatgt tttgagtaca ttcacttcag
     6541 attctagtac agcaagtagt aaataagcgg tataaataaa aaatatgaac tatttaaatc
     6601 aataaaactg ttcatctaaa tctttttatt agaactaaat caacagtagt ttaattacta
     6661 cttgatttag ttctttttct acaaaaaata gcataaaaat ctagttatcc gcataaaaac
     6721 tggacttatc acactttatc aaggtcaaaa ccactcaatt tactactaat ttactactta
     6781 tgaatgagct ttgatacgac gatttatcct tgaaaagtga agatataaag atacttccaa
     6841 taaaatttga atatttaata ggtagacact tcaaaaaatg aggtgtctat ttttttaccc
     6901 gattttgaaa ggaagtgaac ttatgaaaac aaaaaatcaa gaatcaaaag gtcgttcccc
     6961 actctttaag accatcaaac attcattcag ccaataaaaa agaaaggata ggtaaaaata
     7021 tggaacttaa atttgtgatt cccaacatgg aaaaaacatt cggcaattta gaatttgctg
     7081 gcgaggataa agtcgttcag cgaagaatca acggacggct aactgtctta tcaagaagct
     7141 ataatctcta ttctgatgtt caaagagcag atgatattgt ggtggtgctt cctgctgaag
     7201 ctggcgaaaa acatttcggc tttgaggaac gtgtgaagtt agtcaatcca cgtattaccg
     7261 cagagggcta caaaatcggc actcgtggtt ttacaaatta ccttttacat gctgacgaca
     7321 tgataaaaga ataaagaaag agaggaaaaa tgatgagatt agcaaatggc attgtattag
     7381 ataaagacac gacttttgga gaattgaaat tctctgctct acgtcgtgaa gtgagaatcc
     7441 aaaatgaaga cgggtcggtt tcagatgaaa tcaaggaacg tacctatgac ttaaaatcca
     7501 aaggacaagg acgcatgatt caagtaagta ttcctgccag cgtgcctttg aaagagtttg
     7561 attataacgc acgggtggaa cttatcaatc ccattgcgga caccgttgct actgccacct
     7621 atcaaggagc agatgttgac tggtatatca aggcagacga tattgtgctg acaaaggatt
     7681 ctagttcatt caaagctcaa ccacaagcaa agaaagaacc gacacaagac aaatagtcgc
     7741 taggtagaaa ggagactttt tcgcatgaaa cagcgtggta aaaggattcg cccatctggt
     7801 aaagatttag tctttcattt tacgatagcg tcactcctgc ctgttttcct gctggttgtc
     7861 ggactgtttc atgtgaagac aatccagcag atcaactggc aggattttaa cctatcacaa
     7921 gcagataaga ttgacattcc ctatttaatt atcagtttca gtgtcgcaat tcttatctgc
     7981 ttgctggtag cgtttgtatt caaacgggtt cgctatgata cggttaaaca actttaccac
     8041 cgtcaaaaac tggcaaagat gatacttgaa aacaagtggt atgaatctga acaggtcaaa
     8101 acagagggtt tctttaaaga tagtgctggt cgtacaaagg aaaagataac ctacttccct
     8161 aaaatgtatt atcgacttaa aaatggcttg atacagatac gggtggaaat cacgctggga
     8221 aaatatcaag accaactctt acacttggaa aagaaattag agagtggctt gtactgtgag
     8281 ctgacggata aagagttaaa ggattcctat gtggaatata ctttgctcta tgacaccata
     8341 gccagtcgta tttctattga tgaagtagaa gctaaagatg gtaaacttcg cttaatgaaa
     8401 aacgtatggt gggaatatga taagctccct catatgttga ttgctggtgg tacaggtggc
     8461 ggtaaaactt actttatact gacactgatt gaagccttgc ttcatacaga ttcaaaactg
     8521 tatattcttg acccgaaaaa tgctgacctt gcggacttag gttctgtgat ggcaaatgtc
     8581 tactatagaa aagaagactt gctttcttgc attgaaacat tctatgaaga aatgatgaaa
     8641 cgtagtgagg aaatgaagca gatgaagaac tataagactg gcaaaaatta tgcttactta
     8701 ggtctcccgg cacacttctt aatctttgat gaatacgtcg ctttcatgga aatgctggga
     8761 acaaaagaaa acaccgcagt tatgaataag ctgaaacaga ttgtcatgtt aggtcgtcaa
     8821 gctggcttct ttctaatact ggcttgtcaa cgtccagacg caaaatattt aggcgacgga
     8881 atccgtgatc agtttaattt cagagtggct ttaggtcgta tgtctgaaat gggctatggc
     8941 atgatgtttg gcagtgacgt acaaaaggat ttcttcttaa agcgaatcaa aggtcgtggc
     9001 tatgttgatg taggaacaag tgtcatatca gagttttata ctccccttgt accaaaagga
     9061 tatgatttct tggaggaaat taaaaagtta tccaacagca gacagtccac gcaggcgacg
     9121 tgcgaagcgg aagtcgcagg tgtggactga tcttgctggc tggtgtggca atagccacgc
     9181 cagcacttaa ccccccgtat ctaacagggg ggtacaaatc gacaggaaac agtcaaaaaa
     9241 acattagaaa atcctttggt tacaagggat ttacaaaatt tcagcgtatg tcaaatgggc
     9301 tttaaaagtt gacatacgcc tttttgattg gagggatttt tactgaatga acaaacttgg
     9361 ttacagcatt taaaagaaaa acgcttggct tatggactat ctcaaaaccg tttagctgtt
     9421 gcgactggta ttacaaggca gtatctaagc gatattgaaa caggaaaagt caagccatca
     9481 gaggatttac agcagtccct ttgggaagct ctggaacgct tcaatcccga cgctcccctt
     9541 gaaatgctgt ttgattatgt aaggattcgc tttccgacaa cagacgtaca gcaggtggtc
     9601 gaaaacatct tacaactgaa actgtcctat tttcttcatg aggactatgg tttctattct
     9661 tattcagagc attatgcttt aggcgacata ttcgtccttt gctcccatga actggacaaa
     9721 ggagttctgg tggaattgaa aggtcgtggg tgcagacaat ttgaaagcta tcttctggca
     9781 caacaaagaa gctggtatga gttctttatg gacgttttgg tggctggcgg tgtgatgaaa
     9841 cgccttgacc ttgccattaa cgataagaca gggattttga atatccctgt actcactgaa
     9901 aagtgccaac aggaagaatg tatctccgtc ttccgcagtt ttaaaagcta tcgcagtggc
     9961 gaactggtac gcaaagagga aaaggaatgt atgggaaaca ccctctatat cggttcatta
    10021 caaagtgaag tttatttctg tatctatgaa aaggactacg agcagtacaa gaaaaatgat
    10081 attcccattg aagacgcaga agtaaaaaac cgttttgaga ttcgattgaa aaatgagcgt
    10141 gcctattatg cagtccgtga tttactcgtc tatgacaatc cagagcatac cgcctttaaa
    10201 attatcaatc ggtatatccg ttttgtagat aaagacgatt ccaaacctcg ttctgattgg
    10261 aaactgaatg aagaatgggc ttggtttatt gggaacaatc gtgaacgatt aaaactaacc
    10321 acaaaaccag agccttactc cttccaaagg acgctgaact ggctatctca tcaagttgcc
    10381 ccgaccttaa aggttgcgat taaacttgat gaaatcaacc agacgcaggt tgtaaaagac
    10441 attctcgacc atgcgaaact gacagaccga cacaagcaga ttttgaagca acagtcagta
    10501 aaagaacagg acgtgataac aacaaaaaaa taactcaaat acaaattcat tgaatataga
    10561 gaggagaaca tttttatgaa ttttggacaa aacct
//
LOCUS       DACDYI010000090        10574 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.090, whole genome shotgun sequence.
ACCESSION   DACDYI010000090 DACDYI010000000
VERSION     DACDYI010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.090"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            12..84
                     /locus_tag="IWM52_002518"
     tRNA            12..84
                     /locus_tag="IWM52_002518"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="IWM52_002519"
     tRNA            88..160
                     /locus_tag="IWM52_002519"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="IWM52_002520"
     tRNA            168..249
                     /locus_tag="IWM52_002520"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="IWM52_002521"
     tRNA            267..339
                     /locus_tag="IWM52_002521"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="IWM52_002522"
     tRNA            351..422
                     /locus_tag="IWM52_002522"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="IWM52_002523"
     tRNA            439..524
                     /locus_tag="IWM52_002523"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="IWM52_002524"
     tRNA            535..608
                     /locus_tag="IWM52_002524"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="IWM52_002525"
     tRNA            618..691
                     /locus_tag="IWM52_002525"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="IWM52_002526"
     tRNA            711..783
                     /locus_tag="IWM52_002526"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="IWM52_002527"
     tRNA            811..884
                     /locus_tag="IWM52_002527"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="IWM52_002528"
     tRNA            898..971
                     /locus_tag="IWM52_002528"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="IWM52_002529"
     tRNA            995..1084
                     /locus_tag="IWM52_002529"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="IWM52_002530"
     tRNA            1097..1170
                     /locus_tag="IWM52_002530"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="IWM52_002531"
     tRNA            1189..1264
                     /locus_tag="IWM52_002531"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="IWM52_002532"
     tRNA            1266..1336
                     /locus_tag="IWM52_002532"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="IWM52_002533"
     tRNA            1350..1423
                     /locus_tag="IWM52_002533"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="IWM52_002534"
     tRNA            1448..1535
                     /locus_tag="IWM52_002534"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="IWM52_002535"
     tRNA            1557..1628
                     /locus_tag="IWM52_002535"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="IWM52_002536"
     tRNA            1651..1740
                     /locus_tag="IWM52_002536"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="IWM52_002537"
     tRNA            1753..1826
                     /locus_tag="IWM52_002537"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="IWM52_002538"
     tRNA            1845..1917
                     /locus_tag="IWM52_002538"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="IWM52_002539"
     tRNA            1921..1993
                     /locus_tag="IWM52_002539"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="IWM52_002540"
     tRNA            1998..2068
                     /locus_tag="IWM52_002540"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="IWM52_002541"
     tRNA            2081..2154
                     /locus_tag="IWM52_002541"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="IWM52_002542"
     tRNA            2166..2237
                     /locus_tag="IWM52_002542"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     gene            2503..3696
                     /locus_tag="IWM52_002543"
     CDS             2503..3696
                     /locus_tag="IWM52_002543"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAP9974732.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="IWM52_002544"
     CDS             3826..5298
                     /locus_tag="IWM52_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9974733.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="IWM52_002545"
     CDS             5381..6169
                     /locus_tag="IWM52_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAP9974734.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="IWM52_002546"
     CDS             complement(6236..6904)
                     /locus_tag="IWM52_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP9974735.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="IWM52_002547"
     CDS             7019..8731
                     /locus_tag="IWM52_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9974736.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10506
                     /locus_tag="IWM52_002548"
     CDS             8734..10506
                     /locus_tag="IWM52_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9974737.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         3033 a         1926 c         2453 g         3162 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattttt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac ggtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcat
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 agttaaatta aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt
    10561 ttttataagg ggca
//
LOCUS       DACDYI010000091        10432 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.091, whole genome shotgun sequence.
ACCESSION   DACDYI010000091 DACDYI010000000
VERSION     DACDYI010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.091"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(142..894)
                     /gene="xth"
                     /locus_tag="IWM52_002549"
     CDS             complement(142..894)
                     /gene="xth"
                     /locus_tag="IWM52_002549"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAP9974738.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1007..1927
                     /locus_tag="IWM52_002550"
     CDS             1007..1927
                     /locus_tag="IWM52_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9974739.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2000..2935
                     /gene="murB"
                     /locus_tag="IWM52_002551"
     CDS             2000..2935
                     /gene="murB"
                     /locus_tag="IWM52_002551"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAP9974740.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3233..4711
                     /locus_tag="IWM52_002552"
     CDS             3233..4711
                     /locus_tag="IWM52_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9974741.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            4988..7048
                     /locus_tag="IWM52_002553"
     CDS             4988..7048
                     /locus_tag="IWM52_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974742.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            7131..7700
                     /locus_tag="IWM52_002554"
     CDS             7131..7700
                     /locus_tag="IWM52_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9974743.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(7875..8681)
                     /locus_tag="IWM52_002555"
     CDS             complement(7875..8681)
                     /locus_tag="IWM52_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974744.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            8819..10333
                     /locus_tag="IWM52_002556"
     CDS             8819..10333
                     /locus_tag="IWM52_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAP9974745.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         3702 a         1739 c         2055 g         2936 t
ORIGIN      
        1 acagcttttt cacaatctct cgataaacag tgttcaaaag ccagtttctc ggtattatgc
       61 caaatttatg caaaatgtgg aaagccattt tcacaaaatt tttcttaata ctcgaaacta
      121 aacgactttt tcacaacctc tttatttcaa atctaattct actggacaat gatcactgcc
      181 cataatgtcc gtatggatct ttgcatcagc tagtcctggc tctaaatctt tgctcgtcaa
      241 aaagtaatcg atccgccaac cagcattgtt tttccttgaa ttgaatcggt agttccacca
      301 agagtagaca ccttcctggg ttggataaaa ataacggaat gtatcgataa acccgttatc
      361 caacagtcga gaaagagccg ctctttcttc tggagtgaat ccagcatttt tttgattcgt
      421 tttccagttt ttcaaatcaa tcttttgatg agcgacattt agatccccac acacgatgac
      481 tggtttttgt ttttttagat tttccaaata attataaaag gcttcttccc attctaatcg
      541 gtaatctaaa cgttttaatt cgctttgaga atttggcgtg taacaggtta caagaaagaa
      601 atcggaatat tccaaagtaa tcagacggcc ttctgtatca tggatatcga tacccattcc
      661 gtaagtggca ttcaatgctg gttttttagc aaaaatcgct gtgccggaat agccttttcg
      721 ttccgcgtaa ttccaatatt gataataacc tggaagatct aaatcaattt gtcctgcttg
      781 gagtttcgtt tcctgcagac agaaaaaatc ggcgtctaac tcatgaaaaa cttctaaaaa
      841 atttttattt acgatggcac gaagcccgtt tacgttccaa gagataaatt tcaataaaag
      901 cacactcctt cgaattaatt tccttacctt tgcgatacta ttgtacctca ttttagtaaa
      961 aaatggtgta agatgaaagt aactaaatga tccaggagga aagtagatga agattggtgt
     1021 aatcggtgta gggaatatcg cagaaaaagc atatttacca acttatgcga aaaaacaagg
     1081 aacacttgat ttttattttg caacaagaaa taaagaaaca aaagaaagaa ttcaaaaaga
     1141 gtacgggttc caacatctat atgaaacgat cgatgagctt ttagaagaaa aaatcgaagc
     1201 atgtatgatc cacgcagcaa caaaagtaca ttatgaatta gccaaaaaat gtctagaaaa
     1261 tggtatccat gtttatattg acaaaccact gagtgtccat ctaaatgaag ttcgtgaatt
     1321 gcaagaatta gctgataaga accaaataat tttgatgatc ggtttcaacc ggcgctttgc
     1381 tcctatggta gaagaattga aacgtatccc agataagcgg ttgatccacc tgcaaaaaaa
     1441 tcgaatagcg gcaaaagaaa cgacagagtt cgtcctttat gatttgtttt tacatcttat
     1501 tgatacagct gtgtatttat tagatgaacc cattttgaga acaaaatacc agataagaga
     1561 aacaaaggaa tttttggaat atgccatttt acaattagaa acacaaaatc aaacggctat
     1621 tttaacgatg gatttagcaa gtggcgctaa tatagaaaaa tatcaagtaa caagtaaaaa
     1681 tggtacttat gaagtaaacg atctgaccca gcttgtcatt caagatgctg atggaaaaaa
     1741 agtgaaagaa tttggcgact ggacaaacac gctagtcaaa cgcgggtttg agccaatggt
     1801 agaggccttc ttttctcaga tccgtttagg taaatcggaa acagaattgc ttaaacaaaa
     1861 agaagttgta agaagtcatg aattatgtga ggaagtgctt aaagatcact accgtcatca
     1921 actttgaaca agactggcaa gattgtatta agcgtgatag aataatgagg acgtttgaga
     1981 aaagaaagga aaatcctcag tgaataaaga agaaatagtt gaaagcttag ccacgattac
     2041 tatattgttt gatgaaccat tgatgaacta tacgtttacg aaaacaggtg gaccagctga
     2101 tgtccttgca ttcccgaaaa aacaagaaga agtaaagcaa atcatcgatt attgccgaat
     2161 ccatgatata ccttggatgg tattaggaaa tgcaagcaat ctgatcgttc aagatggcgg
     2221 gattcgcggt gtagtcatca tgctgactga aatgaaacag attcatgtca aaggaacgat
     2281 ggtcattgcg gaagcaggtg cctcactgat tgatacgact tatgcagctt tggcagaatc
     2341 attgactgga tttgaatttg cttgcggcat cccgggaagt gtcggtggag cggtttatat
     2401 gaatgcaggt gcgtatggcg gagagatcaa agatgttttt gctgaagtgg atcttttgct
     2461 tgaagatgga acattgaaaa ctttgacaaa agacgaaatg gtattttctt atcgtcacag
     2521 taaagtccaa gaattacgtg ctatcgtatt ggaagctcga ttcagtttgc aaacagggga
     2581 ttatgaagca atcaaagcaa gaatggatga gttgaccgaa ttgcgtcaat caaaacagcc
     2641 attagaatac ccttcatgcg gaagtgtctt taagcgccca gtaggacact atacaggtca
     2701 gctgatccaa caagcaggat tgcaaggcct gaaatggggc ggtgcacaag tctctgaaaa
     2761 acatgctgga ttcatcgtca atatcgatca tgcgacagcg acagattata ttgaattgat
     2821 tgcgcacatc caagaagtaa tcaaagaaaa atttgatgtg tctttagaaa cagaagtgcg
     2881 gatcattgga gaaaaacaag caatatcaga aacaagcaga agtttagcca attaataagt
     2941 aatggaactt aaaataagta aaagagcagg acggtggccg tcctgctctt ttttgtggtt
     3001 gtttacgcaa atggcgcaaa tcttgtacat tttaacacaa taaaacaaaa aatatagaat
     3061 atttcctcat aaatttttcc attttttgca atattctcaa aaaaaagata aaaaatgaga
     3121 ataactgatg aaaaaacaag cataatccat cataaaatca gtataggtga aaagatgaac
     3181 tttcatctgc atatcaagta attggtatga ggcatttttt gaggtgtagg atatgaagtt
     3241 ggattacctt atagaagagg aagatagaga aaaactgctg ctagctagat atttagaatt
     3301 tgagaaaaag aattatgtag tgctgcctaa gttatatgaa acattaggtt tgtctaaatt
     3361 taaaacaaga aattatatca tagaattaaa taaagaattt actgaattca aaacaaagcc
     3421 agagatccaa atttcagaag aagcagagat ccatgtcgaa cgtctgaatt tgacggatat
     3481 caaaaattta agaagaatct actttcaacg atcaaaaatt gctcgactgt taacggagat
     3541 cgtggattca aaagtaacga tcgaaaaatt tgcagagaat cattttttaa gcatatcaag
     3601 agcctatgta aaaagaagag aattagtctc ttttcttgaa cagcatcaaa taaagttaaa
     3661 gaaaaacata ttagttgggg aagaaataaa catacggaac gtattatttg gggtatattt
     3721 cggggtcttt aatggttttt ctatgccatt tagagaaagc gtcatgaatg aagcaaagag
     3781 tatcgtgcaa tattttgaat atctgttcaa tttaaaactg tctgcaacaa aacgaatcaa
     3841 attcaatttg ctaaccacta ttagtttgtt gagggcctat gggaatcaac caatcggaaa
     3901 acgtttcttt tcagatgaat actttaagag ctcagaagga atgaagatca tcaaggattt
     3961 atcacatatt tcttttcttc atatccctaa agagcagatc attgatgaga tttcttatat
     4021 catgttgttt ttgatcacgg aagaagaatt tgaattagat ggcgcatttg gacttgcgga
     4081 agctcatttt catgatatcc acatgattag tcaaaaaatg tcagtggaat tgattgatca
     4141 attaccattt tcgattgaaa tctctgaaga agaaagacta tatatacgaa aagaacttcg
     4201 tatgggtatg atgcatgtca atcgaaaaaa ttggatattc gactttgaga tgactagctt
     4261 tacgtctaaa aagcagctac agtttttctt cgagacgtat ccaatattca gtacagcaat
     4321 ccaaaaggtc atacaagaga ataaacgcta tttcttggga aaagatgaac gtttcatgat
     4381 tcgcttattt tatgattatc tctttttatt ggtcggtatt tgtccggtat catattttga
     4441 agcaccgatt catgtctgta ttgatttttc gcaaggatct gcttataccg attacattat
     4501 ttctcaagtg aaaggattta aaaattacaa tattgtgatt gagagtcgaa tcacttctcg
     4561 tacgaatatt tatttatctg attgtttact tgaaaaattt aataaagaac agataatttg
     4621 gaaaaatcca ccgactccag atgactggga atattttgga aataccatca tcagagaaaa
     4681 aaatagacat ttaactgaaa aaatcagcta gtatttatgg atggcagctt tgtctcacat
     4741 atttattagc tgatttatgt ttttttcaaa gaaaaggtaa ctagatataa ctagatataa
     4801 ctagatatat tacttaacaa aattaaaaag tacataagtt attattccga ttttcttgaa
     4861 acaataaaaa atcaaaaaaa atactctcct aatagtccat cttcttttga ttttatttat
     4921 ataatgaaat catcttatga aaattactac ttcatgttta gacaagagta gggaggacta
     4981 agaataaatg tgttcaaaaa gacgttcaaa acaactaaat aaacacgctt gtcgtttaga
     5041 taaggaacgg tcgtgttctg aaaaacacta cgttaagttt atgatgcgca cgatgctgat
     5101 cagtttatct gtaggtgtct atatgcatgt acccgttcag gcaacgacac aaaccatcaa
     5161 ggtcgtacgt acctctgttt ctgagaccac tgattcccct atcactatta ctcatacttt
     5221 cactgatgca aatggaaaac agctacttcc tgcattgact tcctataata aagtcagtta
     5281 tcctccaacg ataaaccact actggttaat tcctaacaaa acattatgga catcggaaac
     5341 cggaagcagt gtgccgcttt ctatcttttc cgatatgact gatcctacaa aaggtgatca
     5401 gttaaaacag atcactgata ttttgaatca tgctttgaca gatataaagg acggtggaag
     5461 cgcacaagtt tcttatgtat atgaaaaaga ttattcacag tttgaaagaa agaaaatcga
     5521 tgttctggca gggacaaaag tttcgatatc tgatctggta gaaaggctga agaacgcaga
     5581 tggaacacaa ggagatatgt ccaaagtaat ttcaaacaat gggaatatcg tggatacgac
     5641 taatacagga acatctacta tttccttgtc ctattttgat ccaattgctt taaaaacaat
     5701 gactgcatct gctgtgatcc atgttcgtgc tgatcaaaca gctatcaaag gaaaagatct
     5761 tcacttaaga acaggagaga gttacgcccc taaagatctt gttgaatatg taacaaaaag
     5821 cgatggaact tcaggaaata aagaagaaat caaaatgaac gggacagtag atactacaac
     5881 tccgggagaa tatcccgttg tattaactta tatagactcg aaaacatcaa atgtggcaga
     5941 aacaacagct gtagtttatg tagaagcatc tgctgcttca tcgactgtca tcactcattt
     6001 tatagatagt caaacaaata aagaacttca agcagaaaca catatgagta gtgtaaatcg
     6061 tattcctgat ttgcgcctac ctgctgaatt ttctgatcga gtgccaaata tagatttatc
     6121 gacttatgaa caaaatggga aagtcacttc tatttctgat tggatgcaag caaacggatt
     6181 agacagaaaa agtcactggt cggagatcct tgaagtactt cactcagaac tgatgaataa
     6241 taggaagcaa tcaggagggg aaattacctt tacttatgtt tatataccag acaatcgttc
     6301 attagaaggt catgatctta ttgttcatcc aaacgaaaaa ataaacaaag aagagttgat
     6361 ccaatcagca acaaattcct taggtaaacc cgtaccgtta aaggatgtat cggttacaat
     6421 caatgaagta ccattggagg atggttattt gaccaaacaa acaggtgatt ttacgattat
     6481 gtatagttat catgacccgt attcactcca agaactccat gctcagtcac tattacatgt
     6541 tttaccatta aacaaacaga tagatcaaaa agaacaaaaa agacaagaag aaagcttgtc
     6601 agaaaaaaaa caacaaatgg ctcatgcagt ttctccaaca gggaaagtaa aacaaacaag
     6661 aagcctagag ttagcaagaa tatctagaaa aatctcagaa tctaataaaa cgataaggca
     6721 agaagctcat ttggccccac aagtttctgg attggaaaat gaaattttag agcctaaaat
     6781 aaaacaacaa tcagaaaaag aatttgttgc taaacagtcg gaagatcaga aggaatcaga
     6841 tgaccagaag gaaacgaaga aaaaacgctt gtcaactatt gattccaccg atgagcatct
     6901 gcagccgcaa gtaccacttc ctagtccggg aacacccgca cctggtggag gtattccttc
     6961 ttctgcttta ggagatttca tgcgaggtat ggcaggaaca tgggtatacg ctaatcgaaa
     7021 tgaatttgac gcagatgact ggttctgatt tatatctcta tctgttatag tcaaataagt
     7081 agctgagtca tatgttattt ggcataatgg agacgaggag ggatagaaaa atgttagata
     7141 aaacgttacc ttacgcagaa atctggatga caagaccact tcaacaaaat gttccaatca
     7201 cgcctttagc ggaaggtttt gaatttcgat tttatcaaaa agaagatgaa tctgcttggg
     7261 cggagattga gacttccgtt ggggaatttc aaacgacaga tgaagcactt gagtattttc
     7321 aacaaaactt tgctccttat cctgaagaac ttgaaagaag gatgtttttt gtagagacaa
     7381 aaaaaggaga aaaagtagca acctgtaccg cttggcgaaa aaaatcaaga gagcaagtgt
     7441 atccggtttt tcattggtta gcagtgaaac cagattacca aggaaaaggg ctcgcaaaag
     7501 cattgactgc cagagtcttg caagaatttc ctaccctcca tacggacgga ccaatctatc
     7561 ttcacacaca gacatggagc catcaggcga ttgctcttta taaaaaaatg ggttttactt
     7621 ttattgctga aaatctagac ggcacaccta atccagatta tgaaaaagtt atgcaggttt
     7681 taagtaaatt agataaataa cgaaatggtg aaataagtcc tcagtattgg gataaaaaac
     7741 gaaatagtca ttcctcatat cttacttttt tgagttaata ctatcggata actaatggca
     7801 tgccatacat tcaaaaaaga aggggcagtg acaaaagtat tgtcactgcc ccttctcttg
     7861 ttttttagca tgttttatcg tttgattttt ttaggaataa aattcttgat gaacgtaaat
     7921 ttttggtaat cgtttcgact atagaggtaa ccaaatacac tgaaaacaac tgctccaata
     7981 aaattaacaa ataaatcctt catagtatcc atgattccca cgtctaagta accattttgg
     8041 atgattgttt tatctgtttt accatctgca ttttgacttt caatgattgt ccgagtgata
     8101 tctggaacat gatagacagt gtttccgtct ttacttaact ccacactatt gatttgctgg
     8161 atgatcgtat ctttttgcat atcgaattgc agtaatttat ctgctgaaaa ttccacaaac
     8221 tcccacataa caccaactgt catcgaaaaa cagaaggaaa caactgctag taataaagga
     8281 gtcaagttca tggtttttgt atttttattg agcaaataaa caagggaaaa gccgacaccc
     8341 ccagctaaaa atccgctgaa tgcatgtaga aaagtatccc acattgaaaa atagccataa
     8401 aaatcactta actctcctaa aactgttgaa ctaaaaataa aagataacaa aatgatttct
     8461 agcatatttg gaatctgtat ctttaatatc ttttccagaa aataagggat ttcaaataaa
     8521 attaatgtca gtaataaaag cattgtattt ccccagtgtt ggttccagac ctgatccact
     8581 agcataataa cgacgatgat acgtaataaa atatagattc cgtttgtgat tctacctgca
     8641 gtttttgtac tgttcgtaaa aaaggttttc aaatgattca taaaataact ccttcatgta
     8701 tatccttaat tatactaaga agcgtgtgtt taccaaagct atatgactta aagaaaaaca
     8761 gagttgatat tgtgaaaaaa atcacttaat atagtataag gagaaaaggg ggatacaaat
     8821 gaaaaaatta gttgctggtt tattaacagc cgttgcactt cttgctatga gcgcatgcgg
     8881 cgcagatcag aaaaatacag cgcagacaag ttctagtacg aaggaatctg ccgaagtatc
     8941 atcaggcgct tctgaacaag tatatacaga tccaaaagaa atgaaagaat cttatgatgt
     9001 cgtgattatt ggagctggtg gtgcagggat ggcagcagct atcgaagcaa aagatgctgg
     9061 gttgaatcca gtaattttgg aaaagatgcc tgttgccgga ggaaacacat ctaaatcatc
     9121 aagcggtatg aatgcttcag aaacaaaatt tcaaaaagaa gaaggaatca aagactcgaa
     9181 tgatgccttc tatgaagaaa cgctaaaagg tggtggagga acgaatgata aagaaatgct
     9241 tcgatacttt gtcgatcact ctgctgatgc ggtcgattgg ctagatcaaa acggtatcaa
     9301 actggacaac ttaacgatca ctggagggat gagtgaaaag aggacacatc gtccgtctga
     9361 tggctcggct attggtggat acttagtgga ggggttattg aataacgtcc atgagcggga
     9421 aatccctatt tttgtcaacg cagatgtgac agacattaag aaaaatgggg aaacggtcaa
     9481 tgaagtgact gtacatgttc aaggagaaga accaaaagag gtcaccggaa aagcaatcgt
     9541 agttaccaca ggcggttttg gtgccagcaa agaatttatt gaaaaatatc gtcccgattt
     9601 agctgattat attacgacaa atcaagaagg atcaactgga gatggtatca ccatgattga
     9661 aaaattaggt ggacaaaccg ttgatatgga taaaatacaa attcatccaa ctgtgcagca
     9721 ggatgagggg gtcttgattg gtgaggttgt tcgcggagaa ggagcaatct tggtcgatca
     9781 agaggggaaa agatttgtca atgaaatgga tacaagggat aaagtatctg cagcaattac
     9841 agcattgcct gaaaaatcag cttatttgat ttttgaccaa ggcgtgcgtg atcgtgccac
     9901 agctattgaa ttttatgacc aaaaaggata tgtcacaact ggaaagacaa tggaagaatt
     9961 agcagaaaaa atcgatttac caaaagaaac actagcaaaa acagttgatg gatggaatac
    10021 ggatgttgcg gagaaaaaag atgctcaatt caaccgtgcc acagccatgg aacatgactt
    10081 atcgtctccg aattattatg cgatcaaaat cgcaccagga atccattaca cgatgggcgg
    10141 tgtgaaaatc aatacgaaca ctgaagtatt agataaagaa aaccagccaa ttagggggtt
    10201 atatgctgct ggtgaagtca caggtggctt acatggaaac aaccggatcg gtggaaattc
    10261 agttgcggag atcattattt ttgggcgaca agcgggcata caagcagctc gatttgcttc
    10321 ggaaaatgaa tgaaatagaa cgtaaataaa aagaggttgt gaaatcagcg ttttgagcta
    10381 ataccgaggg tcagctgatt gaacactgtt tagtgaagag gttgtgaaaa ag
//
LOCUS       DACDYI010000092        10244 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.092, whole genome shotgun sequence.
ACCESSION   DACDYI010000092 DACDYI010000000
VERSION     DACDYI010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.092"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(407..739)
                     /locus_tag="IWM52_002557"
     CDS             complement(407..739)
                     /locus_tag="IWM52_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP9974746.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKAVVQCKKYI
                     IQKDKVSEEMIYYTGKRKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            complement(1575..2615)
                     /locus_tag="IWM52_002558"
     CDS             complement(1575..2615)
                     /locus_tag="IWM52_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAP9974747.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSSYSEAQQTVQTIHNAKRKAEELEQTIVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(2963..3289)
                     /locus_tag="IWM52_002559"
     CDS             complement(2963..3289)
                     /locus_tag="IWM52_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974748.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNYKPKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(3276..4064)
                     /locus_tag="IWM52_002560"
     CDS             complement(3276..4064)
                     /locus_tag="IWM52_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9974749.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(4430..4720)
                     /locus_tag="IWM52_002561"
     CDS             complement(4430..4720)
                     /locus_tag="IWM52_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP9974750.1"
                     /translation="MSINTFVYSHPINVYIIKYLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(4856..5035)
                     /locus_tag="IWM52_002562"
     CDS             complement(4856..5035)
                     /locus_tag="IWM52_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974751.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLFI"
     gene            complement(5032..5382)
                     /locus_tag="IWM52_002563"
     CDS             complement(5032..5382)
                     /locus_tag="IWM52_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974752.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRANQNLAKEQMRTE
                     EIAEILNASVIKNRSVCIQKEERNAEGYYPDDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(5375..6700)
                     /locus_tag="IWM52_002564"
     CDS             complement(5375..6700)
                     /locus_tag="IWM52_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP9974753.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERSLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKEKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDDEK
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            complement(7071..7655)
                     /locus_tag="IWM52_002565"
     CDS             complement(7071..7655)
                     /locus_tag="IWM52_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9974754.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            complement(7866..8069)
                     /locus_tag="IWM52_002566"
     CDS             complement(7866..8069)
                     /locus_tag="IWM52_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974755.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            complement(8079..8501)
                     /locus_tag="IWM52_002567"
     CDS             complement(8079..8501)
                     /locus_tag="IWM52_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974756.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            8815..9429
                     /locus_tag="IWM52_002568"
     CDS             8815..9429
                     /locus_tag="IWM52_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9974757.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            complement(9453..9803)
                     /locus_tag="IWM52_002569"
     CDS             complement(9453..9803)
                     /locus_tag="IWM52_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP9974758.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(9797..10060)
                     /locus_tag="IWM52_002570"
     CDS             complement(9797..10060)
                     /locus_tag="IWM52_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAP9974759.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
BASE COUNT         3147 a         1895 c         1498 g         3704 t
ORIGIN      
        1 catgtactgc agcacacgac acaatttctt gtgcgtcctc cccattacga gaagacctac
       61 tccttattcc atgagtttgc gtcaaagcct agatccctta gtagctgctc atgattaatc
      121 taggcgctat tctaacttgt ctaacttcta aattccctct tgtttactct gtgcatatta
      181 tttacacaaa ttcatttcac cactatccaa acaagggcat ttactcgatt gaccaatttt
      241 ttacgttacc aatcgtaaaa acctaccccg tcactccccg aagggaattg ttgatagatc
      301 aagtctactc cttatttttc tagtgattct aagttccttt agaagtctcg acaatatttt
      361 caagcaagcg aaaaacgata aaatacaagt tatgagaatg atcaattcag ctgattaaac
      421 gccccatccc ttttgcggta caacgcaaga ctccattttt tcttcgacca atcagtacac
      481 cgttttggtc ataaacatgt ttgattgttg gatatagctc ttttaaaggg ataggatctt
      541 ttaaaattat ttgcttgcgt ttcccagtgt aataaatcat ttcttctgaa actttgtctt
      601 tctggattat atatttctta cactgcacaa cagctttgct ttgcccagtt gatagtttct
      661 tcgtgatttt ctctgtagcg gtacaccgtt taccgtaatg acaaatatct tcgagactca
      721 aaattcctgt atatgacata aagtaatcct ctcactttct ttaaatattt ttaataagtt
      781 tttcttgtta tttttcaaaa atagagaatt gattttcgtt catgacaaac aaaaaagagg
      841 gaacgcattc aaacatgcgt tccctctaat ttcagcgttc gtcatacggc aaagagccac
      901 acggacattt ttataacatt atgcttaggc atagattcat tacaatatca cgtattttta
      961 accttaatag agccaaaaaa agtggtatca cttttgaata tctgttatac tgagttcgtt
     1021 gcgggtaata ccgtaacact gaagggcacg cattaaggcg tcatcttaat gctttcctgt
     1081 aggacactga cggagattac cagtctcccg agccttacag gggcctttct tttttttgta
     1141 ttttcaatta tcacaattta tcgtccaaaa gtgcagaacg aaaatcaatt catcacaagc
     1201 atatcagaca attaaattta aaacaactaa aaatatacaa aaaagtgtaa ttgaatatta
     1261 gcagcactgc ctgaaaagaa gtaatatcag gcttttcagc ttggggaaat aagtgaatta
     1321 cctctcttca tgaattatta taactcaaaa aagcacatat gcaaacacta gttccctatt
     1381 tttttgaatg aaaaataact aaaatatcta acgattatga ctttaaaacc tacgtttgtg
     1441 attgttttca taaactttta atctaaaaac tccggaataa taactattta tttgtaacat
     1501 ttgtaataaa attaaaatca taaaaaagtt ctaatttaaa attgatcatt tttatagtga
     1561 tcgcttttta tcaactactc tagccaattt tccgtactaa ctgttggtaa ttcgttaaat
     1621 gtttctcttt gttcgtttgc aagctttgcc gcaatagctt tttcgacaaa ccaatttttt
     1681 acataaacga aaattagatt ctgcaaattg tttacttttt cagttttttg tttttgataa
     1741 gctttcagta aagtcatata taatcctttg tcagcatcaa ttccataaga ttcaagttct
     1801 tcataaacaa caatagtttg ctctaattct tcagcttttc tctttgcatt atgaatagtt
     1861 tggacggttt gttgagcttc actgtaactt gaagaaaatg tttttatcaa gttcaataca
     1921 ttttctggta caaatgtatt tatactgtcc tctttcatga tgtcgacgaa gttatctagt
     1981 aaaacttgat tttgtaactg atcagtttca gtgtctatta tgtgtctatt agtgtctata
     2041 gaattagaat agaatagatt gtctgcgatt ttcgcaatta cggttgagtc cttgatatga
     2101 ctggattttg ccgtatttgc gattttcgca attacggtgg tatcattgat ttctcgcggt
     2161 tttgccgtat ttgcgatttt cgcaattacg gtggtatcag tattgtcttc ctcttccatg
     2221 attttaaaga cgtcttgatt cgttacttct ggcttgccta agtacaataa attaggcata
     2281 gattcaatct taccatcacg tttatttact cttcctctct tctgtttcag aagacctgct
     2341 ttttctaatt cctttttgat tttagatact ttaccttcgc ggcattgtaa cagtttcatt
     2401 aattcttcaa ctgtgaatat gaagaatata ttatcatcag aatctaccca gttatttttg
     2461 actgaataat caaatcgatc tttaagtagc atataagcaa tttttgcatc atttgataga
     2521 tccatgtatt tttcgttagt aaaaaatacc ttaggcattt gataatattt ttcacgataa
     2581 atttcttttg atttgtaata attaaaatca ttcatagcca atcctctttc tgtaggatag
     2641 cgttttgttc aaaactgtgt tacaatgaat taaacaaata tatctatcca ctatatattt
     2701 tctactgctg ataagtcgcc aaactttgtt aaagcagtag tcttttttta tcttttttta
     2761 gtttacctta aaaaataaat taaagcaaac cgctctattt caaggtttaa gctcatatca
     2821 actatatcac gatattacaa taacattata tcacgatatt gcgatattac aatatgaaga
     2881 taagcgactt atcagctttg atactactgc tttttataaa aaaaatatca cgatattatg
     2941 atatcgtgat attgaatgta aattaaattt tattttgttt ctttgctagt ttatatcctt
     3001 caatcaagta ttcttcaggt atggatttta atagcgcatc ttctataacg tctggtttac
     3061 ttttgtctga ttcagcaata gtcgtccaat atttgagtgc tgacattaga attggattta
     3121 tgcgaatgct atctgctgtt cttacactac ttccttgatc ttttatgact ttacttatat
     3181 ttttcttggt taattcgatt tcttctactt cttttggttt gtagttcttt ttatttttat
     3241 ttcctagtag tctacttact gcagcttgac tgcgattatc ttctgccatc aaaatcacca
     3301 acttctttta aatattcttc tgtaaatgct ttatagtcat cttcagctgt tgagggttta
     3361 ttaaaattga ttagatctga tccagcagct tgcgcttcgt caacggttat tgtcctacga
     3421 atataagttt tgaaaatttt tgcaccaatg actctagaaa tttcggttgc tgcagacgtc
     3481 atttctttag aaatgttata acgcttatta tacttagtaa ataggactcc tcgtacttca
     3541 agcttgtcgt tcgtatattc ttttaccgtt tcaatagttt cacttaactt tattagtcct
     3601 tttaatgaga aatacgcagc ttcaactgga atgataattt catcagaaac agtaaatgcg
     3661 ttgactgtaa gcattcctaa acttggtgga caatcaatta taatataatc atagttgtcc
     3721 ttgattggtt gaagtactct tttaagcctg tgttcacttc ctacttgagt aaattccctt
     3781 tctgcagaag aaagggctat gtcggctggc aaaacatcaa ttcctttata gtccagaata
     3841 gcatctgtaa tgtcagcctc cccttttaat acttcataaa ttgtaggttg gtttgttcca
     3901 tctatatcga aacctacatt atctgtagca tttccctgag gatcaatgtc cactaaaagt
     3961 acatttttac cgtaaacaaa tttcaaacat gctgctaaat taacgctagt cgtagtttta
     4021 gaaactccac ctttttgatt ggctactgac aatattcttg ccattaaaat atcccccatt
     4081 cgtcttatat tataatataa ttatatcatg atattgtgat attgcaatat cgtatttgca
     4141 ttatattgta atattgtgta attattttgt tttaaagata tatatcggtt cggatataaa
     4201 gatcattcat atgttattcg aaattaaaat aatcactttt tttaatttaa aattttttac
     4261 taatgataag aaatatgttt tgtgagcagc aactagaata atttaaacat tactaaactg
     4321 tctatattgt tactaataat tcccttctta tagtgcattt tcttagaaaa aacaatatta
     4381 cgatatcatg atattaagat atcgtaatat tatacaattg tgattgatat tatatatgtt
     4441 tttttatttt tttatcatgt ttaatcttga aggaatcata ttctttttct aaagacagga
     4501 gcttttcata aacatcagac atgttcaaac tagaggccag gcttaaatca taaacgaatg
     4561 aagcaggtaa tgattttatt ctacgttgtc gggttatcca gcttgcaacc gttccttgtg
     4621 ggtaggcata taactcacaa aattgttcta cagtgattcc taaatatttg ataatgtaaa
     4681 cattgatcgg gtgcgagtaa acaaatgtat tgatggacat ggtgcgtaag cctactttct
     4741 tttattattg cgtttgcaat aatagtttaa cacacgcatt attctagtat ctactcctat
     4801 ttgtaaaaac aaaaaaggat tcatagagat aactctatag aatccgtaaa ataccttata
     4861 taaataacgg gaaaatataa aacctaaaat tatgataacc tgctgcagct tctacataga
     4921 aagagaaacg ctcaacacca acctcattta tcaaacgttg gccatacccc caaatctctt
     4981 ctgatagctg atcaaaggtt atatacttta gctcatattg ctggataatg ttcattctaa
     5041 tcgactccat ttattatcct ggtaaaattc gatatgacgt atttcgtcgt agtgaatttt
     5101 gtctttgcca acgtagattc ccagctcatc actacctaag atctttccaa caatatcgtc
     5161 tggatagtat ccttcagcgt ttcgttcttc tttttgaata catactgatc tattttttat
     5221 gactgaagcg ttcaatattt ccgcaatctc ttcagtcctc atttgttcct ttgctaaatt
     5281 ttgatttgca cgttgctcat tatctgcatc gatctcttca gtatgatccg ataaatagaa
     5341 acctgcccat ttcaatcgtc ctcgatcgtt atagtcaatc atttttaagc ccccctgcgc
     5401 cgcctgcatg gccaccaacc aacgtgcttc tagtaatact acgagcgcct tctaacatcg
     5461 aagaagcatg tacaattgac gtaaaaccat acttctgcct aattttatct attattttgt
     5521 ctagtttttc gtcatcaatc tgtttttgtg gatcactaaa caaatccagc tgaagcgaat
     5581 cagtataaac tagcttgcta taggttattc caacgtgcct tacttcttgt ccttcataat
     5641 acttatcaaa caagaaaaga caataattct gcaattcctt tgtattatct gtgataggga
     5701 ttttcatctg gtgtgaaaat cctggtctcg tttctaagat cgacgtacca atgtttaaat
     5761 ggacacatcc tgttttgcaa ttatgctgcc tgatacgaat ggctacttgt tccgccattt
     5821 cttttaccac cagctcgatt tcatttcttc tagtataatc tcgtggtaac acttgtgaat
     5881 taccataaga tttttccttt gtcggttctg gcgggattgc tatatctgtt cgatcgattc
     5941 cgttagcatg gaagtacagc tgtaatccaa tcacacctaa tctattctta atcgtataag
     6001 gattccagtt agcaaggtct ctaatgctca aaattcccat ttggttgagc ctttttttca
     6061 ttcgcgaacc aattccccaa aagtccgtca tttcgggaat attccatact ttctcaggaa
     6121 cgttttcgta cgtccattcc gcaataaatc cttcattatg ctttgcttca ttgtctaagg
     6181 ctaatttagc caaaagggga ttatcgccga cgcctaccgt agcaatcaaa cctaactctt
     6241 ctttaatacg ttcttggatc atttgagcga gcttcttcct tcgctgactt cgtgtttctt
     6301 cagtcgtaaa aagattcagt gatcgggtca cttttaagat tgattcatcg atcgagtaaa
     6361 tcagtagatc ttcatcagcc acatatcttc tgaaaatatt atttacctgc atatttcgct
     6421 tgatatatag gttcatacgt ggtggaacaa cgtgtagtgt tttaggaaat ggttgtggta
     6481 agtcacgtgg tctactcaca ttagaaatcc catatttttc ctttgcagcc ggtgaagaag
     6541 ctaaaatcaa tcctgatcct gtattgtccg ctcgactcat cacgactaat tcagtcgtta
     6601 atggatctaa actcctttct atacattcga cacttgcgta aaagcttttc atatcgatta
     6661 aaaagtaatc atttactggc tcttttgaat aatccatcat aaataacact ccattctttc
     6721 acattataca aacatacgtt cgatattgta aagaagaaat agctaaatac ataaaaaaag
     6781 aagcataggt atgctatcac cctgcttctt cttatgattt gtatgatcaa tttattctat
     6841 cactttacta attaatttga aaatctaata aaaactctca ctctaaaaga gtgagagact
     6901 gcaaattaga gtacggactt ttttggagtt ccgttcttta atattaacaa atgaataaaa
     6961 tccttacaac atttgagaat aagttatcag tagacactta caaaaaattc tcatactatt
     7021 gtcacttttc tagataaatg gcaaaaattt tcagcattaa atgacaaaaa ctaatgagaa
     7081 tttgtctttc catttttctc ctccattttt gccaaataac gataaatggt ggttcttgag
     7141 acgttccacc gttccgcaat agtcttaata ggtgtaccgc tatccactaa agtcttcagt
     7201 aattccaaat cttgttcaga caatttttca ggacgaccac caaaccgtcc tcttgcacga
     7261 gcagctgctc gtcctgctgc ggatcgttcc aggatcaaat tacgctcaaa ttctgcaaat
     7321 gccgcaaaca aatgaaacat gagctgtcct gtagaacttg atttgtccat cgtaatattt
     7381 tcttgaatac tatgaaaact cacaccacgc tcattcagtc gattgacgat tgaaatcaaa
     7441 tcttccatat ttctacctaa acgatctaag cgccaaacga caagtgtatc gccagagcga
     7501 acaaaatcaa tggccatttc taatcctggt cgattacttt ttgccccact catatgatca
     7561 gtaaaaattt tttcacaacc aaatttattt aggctgtctt cttgtaaatc caaattttgt
     7621 aaaccagtag atacccgggc gtatcctatt ttcaaaaaaa tcactccttc attaatttta
     7681 ttgtaacata actttatcca tatagtttaa ctgaacatag atattgttat gggtttatga
     7741 acatgaaatg aattgttttt ttgaaaaata aagcaaaaaa agaaagtgtc cagaaatggg
     7801 tgattttatg aacactttca attagaaaaa aagaatgtcg actagacact ctttttttaa
     7861 aatcattatt tttctttttg atatgttttt tggtctcttt tttttctttt ttcttcagaa
     7921 tcttctagat tagaaattga tttttgaatt ttttgtgtct cctgtaaagt tcgctgaact
     7981 tgtaaagctg attcttcttt cggtaaatta attttactta gaatatcgct atcttgataa
     8041 aaatttggtg tgttcacaaa ttctggcata aaaaatccct actttctagt taaggttcct
     8101 gaataagtcg caatccaatt gtttccagct gatttataac tttgtaaata caattttcca
     8161 tagtaccaat aaccatttct tttttctttc caattccgtg attgaggagg tctagaaggc
     8221 caaccaactt tatcaaaaat catcgttcct gtcactcgta tcgttttaga agctgctcga
     8281 gcaagtaatg gagaaactat taaataattt tcagtaacag ttaagtcgtt agttgggtta
     8341 ccaatggcta ttccgttcct atctaaagga gttaattctt ggttattatc tactacagta
     8401 gttggttcat ttgtactatc agcaaatgta ggaattgtag ttccagttac actaattcca
     8461 atagttaaaa tacctaaaga taaaataaac ttatttttca ttgtctatca cctcgaaatc
     8521 aaaaataaga aaacgttttc ttaataaatc ttaacagata aatcaagtta tatactagaa
     8581 aattcataaa atatattttt aatgtcataa tcgtttaaat tttcttgatt gacatttatt
     8641 tacaaaaaat attgccattt aaaaagaaaa gtgacacata ctatttcctc catcattagt
     8701 ttttgtctat ataaggtata ccctaactag acacgaccgc gacattgaaa aatctggttt
     8761 tctcgtgtat gattagggtg ttttttaaat agttgcagaa agtaggtaaa aattttgaga
     8821 gttgcataca tacgtgtctc atccatcgat caaaatgaac aacggcagat tgaagaaatg
     8881 aaaaaattcg gtgcagagag aattttcatt gagaaacaat ctggcgcaac gattactcat
     8941 cgacctgttt ttcaagaagc actggatttc gtccgagatc aagatatttt tattgtggaa
     9001 gctattgatc gacttggaag aaattacgac gagatcatcg cttcagtcaa ttacttgaag
     9061 aagaaaaatg tgcagctgat catcacaagt ctaccgatca tggccgaagc aattggcaat
     9121 cctctcctgg ataaattcat taaagatttg atcattcaaa ttttggccat gatcgctgaa
     9181 caagaacgta ccgaatctaa acgacgtcag gcacaaggca ttaagattgc aaaagcgaac
     9241 ggtgtttata aaggacgtcc taagctatac agtgccaatg caaaagatcc ccaacgtcgt
     9301 cttgtttata aaaacatcgt tgaggatcta aaaaaaggtg ttgcgatcgc taagattgca
     9361 aaagactaca atgttacgag acaaaccgta tatagaataa aaaaagacag catggtcaat
     9421 cacgaatagt tgatcatgct gctgtttcta ttttatttgc ttactttttg aaaaactgtt
     9481 ctaactttca tcaataactc ctcaactaaa ctatcatgcg ctttttccaa aaaaatacac
     9541 tctctggctt cataatcaat tgtcgttagt tgatctaata atacttttcc tgttgtcttc
     9601 atttcatatt ctgagtttat atttacatat agcggatagt ttctagttgt actagaaatt
     9661 ggagcaactg taacaacgtt actataatcc gcaataatat catgacttaa aacaatataa
     9721 ggtcttttac ctgtctgttc gtgtccagat cgaggtgctg tattcaataa tagaatatcg
     9781 ccttgtctag gttttctcac catttttcat tacctactgc ttcacctaaa ttttgaatcg
     9841 tcacatgatt tctttcatca gtgtaatttt caaataactg ttcaattgtt aattcatttt
     9901 ctatattagc cgcttcgata attagccgct tctgaccgtt tacttcttct tgtattatct
     9961 tcactttgtc ttctgtatga attccaagat attccataat atactttggc aatctaaggc
    10021 cattactatt tccccatttt tttataggta acatttccat agtagacacc tctttctgta
    10081 tatacatagt ataacataca taagaaaaaa cgtatatact taattatcct tttctattaa
    10141 attagattaa cattccatga atccataaac tatgttaaca tttatatgaa aatagtcgaa
    10201 aggaaatcat aatatgttta agaaaacaaa atctttagaa tcag
//
LOCUS       DACDYI010000093        10139 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.093, whole genome shotgun sequence.
ACCESSION   DACDYI010000093 DACDYI010000000
VERSION     DACDYI010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.093"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            122..628
                     /locus_tag="IWM52_002571"
     CDS             122..628
                     /locus_tag="IWM52_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAP9974760.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            645..1544
                     /locus_tag="IWM52_002572"
     CDS             645..1544
                     /locus_tag="IWM52_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAP9974761.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1631..2515
                     /locus_tag="IWM52_002573"
     CDS             1631..2515
                     /locus_tag="IWM52_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP9974762.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2707..2778)
                     /locus_tag="IWM52_002574"
     tRNA            complement(2707..2778)
                     /locus_tag="IWM52_002574"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2743..2745),aa:Arg,seq:ccg)
     gene            2838..4172
                     /gene="rpoN"
                     /locus_tag="IWM52_002575"
     CDS             2838..4172
                     /gene="rpoN"
                     /locus_tag="IWM52_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAP9974763.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4375..5412
                     /locus_tag="IWM52_002576"
     CDS             4375..5412
                     /locus_tag="IWM52_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAP9974764.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            5453..6454
                     /gene="gap"
                     /locus_tag="IWM52_002577"
     CDS             5453..6454
                     /gene="gap"
                     /locus_tag="IWM52_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP9974765.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6564..7754
                     /locus_tag="IWM52_002578"
     CDS             6564..7754
                     /locus_tag="IWM52_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAP9974766.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7858..8613
                     /locus_tag="IWM52_002579"
     CDS             7858..8613
                     /locus_tag="IWM52_002579"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAP9974767.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8729..10027
                     /gene="eno"
                     /locus_tag="IWM52_002580"
     CDS             8729..10027
                     /gene="eno"
                     /locus_tag="IWM52_002580"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAP9974768.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3549 a         1869 c         2100 g         2621 t
ORIGIN      
        1 atttttgtca caccttcttt tttgtatata aaaatcccaa gtaaatacat aaaaaataaa
       61 aaaattgaaa cttttagcgc accttgtacg tcatattaag tgaggcagaa aggagtggag
      121 aatgattttt actggatata agaaaaaacg gcagatcgaa aaataccgtc acctgctgaa
      181 tggactcatc gaaactgatt atcaaaaaat gttccgtatc acgatgaatt acgtacatga
      241 taaagaagag gccttagatg tgatgcaaga tagttttcat aaagccctga actattttga
      301 aaaagaaaaa gagatcgagc attttagtgc atggttttat cggatattga tccgcacggc
      361 attagatagt tggcgcagac aaaaaaggac gcctgtggac ttatttgaaa ttcaagatat
      421 cccccaacag ttacctttag aaacttccgt tgcagagctc catatgattt tagataaaat
      481 agacagtccg gaaaaagaga ttctgatcct ctactttttt gaaggatttc gtttgaaaga
      541 aattgcgact atcttggata tgaatgaaaa cacagttaaa acgaaaatgt accgctcatt
      601 gaatgcttta cgtcaaatcc tagaatgaag gagagaagag tgccatgtat tcccaaaaag
      661 aaaatttaaa aaaactaaag aaaacttata aagaacaagc aattcccaaa gggcttaaag
      721 aggagatccg ccagcgtttt attatcgaag agcaatcatt tataaaagca aagcggagaa
      781 agagacgtct ccaagggttc ggagtatctt tggctatcgt aatgattaca acaagtagcc
      841 ttctttttaa tagtcaagtt cgaagctttg cagaagattt acccatctta ggatcagtca
      901 ttgaattgat cttaggcgaa cgctttactg atcgatctga aaaaatcgat attcaggtac
      961 cgaagatcaa tacacaaaat gagaaagaaa ataaaacaat tcatggatta aatcaaaaat
     1021 actttcgcga aggacaagca gattttgaaa aagctgaaaa agaatatgga aattttgaaa
     1081 cggatcatta ccaagttttg ggagattatc agaaaatcgt agacgataac cggtttctcg
     1141 tgattgaaag gcagatcacc caaacagcag cagatagcca tgttgaaaaa agatacgaca
     1201 caatcgacaa gaaaaacagt gtccagctct cacttccact tttattcaaa gatgacacct
     1261 atctatctgt tttgacaaaa gaagtcaaac ggcagatggc cgaacaagtc aaggaagatc
     1321 catctaagta ctattggact gagcaagata ttcaagaagg gacgatagaa aaaccaacac
     1381 ttgttacgcc tacacgaagt ttttatttga acaaagagca tcaattagtt ttgacttttt
     1441 ctcaatacga aattgctcct ggttatatgg gtacaccaga atttgtcatt cctaagtccg
     1501 tgacaaaaac gattttggca tcggaagatt atttggatca ttagacgaga agtagttgcg
     1561 actagcagtt tcaattttca agtaagtaca agtaaataat aagaagagaa aaagagaaag
     1621 aagagatata gtgaaaagaa attatttagt tatcagtatt attgctttat ccgtgctttt
     1681 gtttttcgaa attacttcgg tggcctcctt gatcaccgaa gtaaaaggac aaacacactc
     1741 ttcggagcat tcgtcacaat ctttcaaaca atcagaacaa tcaaccatta aaagccaatc
     1801 aactgaaaca gctaacgtca aagaagccat tcaagaatca gcggaaaagg tacaaagtca
     1861 gtcatcacaa tcaacacaag acagtacaaa aacgagccga gatccggctg tcaaaagcgt
     1921 cgcaatcagc tttgatgatg gaccaggagc gaccacaaca ccgcagcttc tgcgaatctt
     1981 aaaagaaaaa aacgtgcatg ccactttttt cgtcttagga gaaaatacgg cgcagcatcc
     2041 tgaaatcgtg aagcagacag ctgaagcagg gcacgagatt ggcaatcaca catacgatca
     2101 tcagaatctg gccactcttt ctgcccagtc gatgacggag gaagtgacga aagcagacac
     2161 tgagatcaaa aaagtcactg gcaaaacccc cacttttgtt cgtccgccct atggttctgt
     2221 cacgaatgta ggagcaacga tcatccagcg accgatcatc gaatggtctg ttgattcaga
     2281 agattggaag acaagaaatc ctgatttgat tttgcagaag atccaagcaa cagtatacga
     2341 tggggcaatt attttattcc atgatatcta tcctgaaacg attcgtgccg ttcctcaagt
     2401 gatcgattat ctgaaagagc aagggtatcg catcacgaca gttggggatt tattagggca
     2461 tccaacggct gtcgagaact attatggcag aaacgatcat cggccagttc agtaagtgag
     2521 acaagtatct ttaataaata acacatttta ataagtagtt gaacacataa ctaccttact
     2581 tgcaaaggct taacaatatc tatacaggta caacaaaaaa ctgtccacca attttctttg
     2641 gcggacagtt ttcacttaaa aataaagaag caactattcg taaattatgc taaaatagca
     2701 gtagaacgcg tcctgaggga atcgaacccc cgtctcaaga accggaatct tacgtgatat
     2761 ccactacact aaggacgcaa gcactgggat aattgtaact aaacagataa aaaattacaa
     2821 ggggattgga caaaaaaatg aaatttgagc aaaatttttc acaaaaacag cagcagacac
     2881 agaaactagc catgacgcaa cagttgcaac agtccatcca agttttacaa tataacagtg
     2941 aggagctgtt ggcattcgtt gagaatcaag caatggaaaa tccattggtg gaagttgttg
     3001 agccggaatg gcagccagat tacataaaag cttcttcatc ttcttatgaa ggagaagaga
     3061 caaattacct aaatcagatt cctgatacaa aagggtcttt atttgattca ttgatcgaac
     3121 aagtccattt gaattaccga gatacttttt tgaggaaaat cgttctttat cttgtagaat
     3181 acatcgattt gaacggattt ttgacgatta gtttagaaga agccggaaaa gagattggag
     3241 ctacgcctat ccaaatgctc gatgcgttga ctctgattca gcaattagaa ccagctggag
     3301 taggtgcacg tagtttgcaa gaatgtctca tgcttcaaac agaacgagat gattatgcgc
     3361 cggaattagc ttatatcgtg ttggaagaat gttttgagga actggtggag cgaaaatgga
     3421 aagaaatcgc acaacgtttt tctgttgatc tacatgctgt tcagcagata ttcgactatt
     3481 tacaaacgtt gagtccaagc cctggccgga ttttcgaccg tagcagcgaa ttgtacatta
     3541 ttcctgatgt ccgggtatta gtggacgacg ataaaaatgt acaagttatc tcaaaccgaa
     3601 aaaatcagcc aaatatacgc tttcaagaga gttacttcaa acaaatgtca caacaagcag
     3661 ataaagaaac tgaaaattat ttaaaagaac ggaaacagga attcgaatgg ttgaagaaaa
     3721 cgatcctaca acgtggggat acaattttgc gggttgctca agtcattgtt tcccggcaaa
     3781 aagctttctt tatcgacaag gaacgtccaa tcaagcctct tacgttaaag gaagttgcaa
     3841 ctgagataga tgtgcatgaa tccacggtca gtcgtgcagt caacgggaaa tatttggaaa
     3901 cagacttcgg tgtatttgaa ttaaaaaaat ttttcactac acgaattcct acgaatagta
     3961 cagaacagac agaagacctt tcagcagata cagcgaagaa aaagctgcag gaattagtcg
     4021 atcaagaaga caaaaacaag ccgttgtctg accagaaatt ggttgaattg ctgaaaaaag
     4081 atgagatagc gatctcaaga cgaacagtag caaaatatcg tgatttatta ggaatcccga
     4141 gttcatcaaa aagaaaacgt tatgataact aatcagtcaa aagactagaa aaagtatttc
     4201 actttttcta gtctttttag ttgaaaaacg agagcaaaac tgttagactt acagtcgtga
     4261 ggttgagatt tccctttatt gatgtgaaat tcgaatagtt aatttctttt tttatcgtat
     4321 atgggtcgtt ttaagtctac attggacgta aagtgtccaa ctaaggagat cgctatgctg
     4381 gatgaaataa aaatgattga atctgttgca ccagatatta tcgaagtgtt gcaagaacgc
     4441 tataaaattt tacgaaatat ctattggatg cagccgattg gacgtcgaag tttatcggaa
     4501 agtatgggga tcacagaacg agtattgcga acagaaacag atcttttgaa aaatctgaat
     4561 ttgatcgaca cctcaaaaag cggtatgacg ctgacaacta agggcgaaga agtttatcaa
     4621 agccttgaaa acttcatgga tcagctttta ggtacgcatc aaaccgaaca acagttggca
     4681 gaatattttg gtaaccaacg ctgtatcgtt gtttcaggaa acagcgaaga gcagaccaaa
     4741 gtggctgatg cattcggcga agcgctgagc gaagccctag accgactact tccagaaggc
     4801 gagaatatca tcgctgtgat gggaggaact actatggctg tggtagccga acaattgtct
     4861 aatctggaaa ataagaaacg gcataacctg tttgtccctg caagaggcgg gattggtgaa
     4921 gcgataactg tacaggcaaa ttctgtcagt gcaagaatgg ctgctaaagc aaacggtaac
     4981 caccgtgcac tttatgttcc ggaacaatta agtctggcaa cttacaattc tttactcaat
     5041 gagccatcta tccaagaagt cttaaacttg attagtgaag cgaattgtgt tattcatagt
     5101 attgggcgtg cattacacat ggcagcacgt cgtaagatga ctgaaaaaga attagtcatg
     5161 ctgaaacaag caaatgctgt tgcggaatct tttggctact ttttcaatga aaaaggtgaa
     5221 gtcgtttata aggtccctag aatcggcata gaattgcaag atttggaaaa agtcccgatc
     5281 atcatggcaa tcgcgggcgg aaaatcaaaa gctaaagcaa ttcgagctta tatgaaaaat
     5341 gcaccaaaac aaacgtggct catcactgat gaagctgctg caaatgagat tttaaaaggg
     5401 gtaacccttt aaaataaata tttttttgat tttcataagg aggaaatctt aaatgacagt
     5461 taaagtaggt attaatggtt ttggacgtat tggacgtcta gcattccgtc gtatccaaga
     5521 tgtagatgga atcgaagtag tagcaatcaa cgacttgaca gatgctaaaa tgttggcaca
     5581 cttgttaaaa tacgacacaa ctcaaggacg tttcaacgga acagttgaag ttcatgaagg
     5641 ttcattcaac gtaaacggaa aagaagttaa agtattagct aaccgcaacc cagaagaatt
     5701 accatggggc gaactaggcg tagatatcgt tctagaatgt actggtttct tcacttctaa
     5761 atcagcagct gaaaaacatt taacagctgg tgctaaacgc gttgttatct ctgctcctgg
     5821 cggaaacgat gttccaacaa tcgtttataa cacaaaccac gaaacattaa ctggtaaaga
     5881 aacagttatc tcaggtgctt catgtactac taactgttta gctcctatgg ctaaaacatt
     5941 gaacgacaaa tttggtgttg ttgaaggatt aatgacaact atccacgctt acacaggtga
     6001 ccaaatgact ctagacggac ctcatcctaa aggtgacttc cgccgtgcac gcgctgctgc
     6061 tgcaaacatc gttcctaact caacaggtgc tgctaaagct atcggtttag taattcctga
     6121 attgaacggt aaattagacg gagctgctca acgtgttcct gtaccaacag gttcattaac
     6181 agaattagta acagttcttg aaaaagaagt aactgttgac gaaatcaatg cagcaatgaa
     6241 agaagcttca aacgaatctt acggatacaa cacagacgaa atcgtttctt cagatatcgt
     6301 tggtatgact tacggttcat tatttgatgc tacacaaact aaagtaatga cagttggcga
     6361 caaacaatta gttaaaactg ttgcttggta cgacaacgaa atgtcataca ctgcacaatt
     6421 agtacgtact ttagaatact tcgctaactt ataagataga caaacgtaca agtgtaaatt
     6481 atagacatgt acatttaaaa gcggggaagc aatcgcgctt cttccgcttt ttttgataat
     6541 aagtttattc aggaggaaat aaaatggcta agaaaacagt gaaagatatc gatttaaaag
     6601 acaaaaaagt tcttgtccgt gttgacttca acgttccttt gaaagacggc gtgatcactg
     6661 acgacactcg tatcaaagca gcattgccaa caatcaacta cgttctagaa caaggcggaa
     6721 aagcaatcct tttctctcac cttggacgtg taaaaacaga agaagataaa gaaggaaaat
     6781 ctttagctcc agttgcaaaa cgtctaggcg aattattagg taaagaagta acattcgtac
     6841 ctgaaactcg cggagaacaa ttagaagaag caatccgcaa catgaatgac ggcgacgttg
     6901 tcgtattcga aaatacacga tttgaagata ttgacggtaa aaaagaaagc aaaaatgata
     6961 cagaactagg taaatactgg gcttcattag gcgatgtatt tgttaacgat gctttcggta
     7021 cagctcaccg tgcacatgct tctaacgtag gtatcgcttc aactggtatc ccaacagttg
     7081 ctggattctt gatggaaaaa gaaatcaagt tcatcggtga agcagtagaa gaaccaaaac
     7141 gaccaatgat tgcaatcctt ggtggcgcaa aagtttctga taaaatcggc gtaatcgaaa
     7201 acttgattcc taaagcagat aaaatcttga tcggtggcgg aatgacttac acattctacg
     7261 aagcaaaagg aatcaagatc ggtaactctc tagtagaagc tgataaagtg gaactagcaa
     7321 aagaattaat cgaaaaagca ggcgacaaac ttgtcttgcc aatcgacaat gtctgtgcac
     7381 cagaattttc aaatgatgtt gaaacacaag taatcgaagg agacattcct gatggtttga
     7441 tggctttaga catcggccca gcatcagtaa aattatttgc tgatacatta caaggtgcaa
     7501 aaacagttgt ttggaacgga cctatgggtg tatttgaaat gagtaacttt gctaaaggta
     7561 caatcggtgt ttgtgaagca atcgctaact tagaaggcgc aacaacaatc atcggtggcg
     7621 gagactcagc tgcagcagca gaacaattag gatttgctga caaattcaca cacatctcaa
     7681 caggtggtgg cgcaagcttg gaattgcttg aaggtaaaga attaccagga ctagcagcaa
     7741 tcaacgataa ataaacgacc aagtttgctc aattctgacg agtaatcgat caaaaggtga
     7801 agggagtttt catgactccc tagccatttt tcaataaatt aaggagtgtg ttcctcaatg
     7861 cgtaaaccta ttatcgcagg taactggaaa atgaacaaaa ctgcatcaga agcaaaagaa
     7921 tttgctgaag cagtaaaaaa caaaatccct tcaaatgaca aagttgattc agtcatcgga
     7981 tctcctgcat tattcttaca agaattaatg acatcagctg aaggtacaga actaaaaatc
     8041 tcagcacaaa actgctactg ggaaaactca ggtgcgttca ctggtgaaac ttcaccagca
     8101 gctcttgctg atctaggtgt tcattatgtg atcatcggac attcagaacg tcgtgaatat
     8161 ttccatgaaa cagacgaaga catcaataaa aaagcaaaag ctatctttgc aaatggtatg
     8221 acaccaatcc tatgctgcgg cgaatcttta gaaacctatg aagcaggcaa aactgctgaa
     8281 tggatcgaag gacaaattac agctggttta gctgatcttt cagctgaaca agtgtcaaac
     8341 atggttattg cttatgaacc aatctgggca atcggtactg gtaaatcagc tgacgcacaa
     8401 atcgctgacg aaatttgtgg agttgtacgt caaacagttg aaaaacttta tggaaaagaa
     8461 gtatcagaag cagttcgtat ccaatacggc ggttctgtaa aaccagaaaa tattgctgaa
     8521 tacatggcaa aagaaaatgt tgacggtgct ttagtcggtg gcgcaagctt gcaagcagat
     8581 tcatttttgt cattgttaga cgctgtaaaa taattgttga aaatttgcga aaaatgcaag
     8641 aaatgtttgc actaatgagc aaaattaact aaaatcgtat atgagggaaa tcccttagga
     8701 aaaaacaaac tcaaaggaga gacaaaacat gtcaattatt actgatgttt acgctcgcga
     8761 gatcttagac tcacgtggta acccaacaat cgaagtagaa gtatacacag aaagcggagc
     8821 ttttggccgc ggaatggttc cttcaggtgc ttcaactggt gaatacgaag cagttgaatt
     8881 acgtgacgga gacaaagctc gttatggcgg taaaggtgta actaaagctg tcgacaacgt
     8941 aaacaacatc attgctgaag caatcattgg ctacgatgtt cgtgaccaaa tggctatcga
     9001 caaagctatg atcgctttag acggaactcc taacaaaggt aaattaggtg cgaacgctat
     9061 tttaggtgtt tctatcgctg ttgcacgtgc agctgctgac tacctagaag tacctttata
     9121 ccactaccta ggcggattca acacaaaagt attgccaaca ccaatgatga acatcatcaa
     9181 cggcggatca catgctgata actcaatcga cttccaagaa ttcatgatca tgcctgttgg
     9241 cgctccaaca ttcaaagaag ctctacgtat gggtgctgaa gtattccacg cattagcttc
     9301 aatcttgaaa gctcgcggat tagcaacttc tgttggtgac gaaggtggat ttgctccaaa
     9361 ccttggttca aacgaagaag gttttgaagt aatcatcgaa gcaatcgaaa aagctggcta
     9421 tgtacctggt aaagacgttg ttcttgctat ggatgctgct tcttcagaat tctacgacaa
     9481 agaaaaaggt gtttacgtac tagctgactc aggcgaaggc gaaaaaacaa ctgacgaaat
     9541 gatcaaattc tacgaagaat tagtttctaa atatccaatc atctcaatcg aagatggatt
     9601 agacgaaaac gactgggacg gattcaagaa attaactgac gtattaggcg acaaagttca
     9661 attagttggt gacgacttgt tcgtaacaaa cactcaaaaa ttatctgaag gtatcgaaaa
     9721 aggaattgct aactcaatcc ttatcaaagt gaaccaaatc ggtacattaa cagaaacttt
     9781 cgaagctatc gaaatggcta aagaagctgg ctacacagca gttgtatctc accgttctgg
     9841 tgaaacagaa gattcaacaa tctctgatat cgctgttgca acaaatgctg gccaaatcaa
     9901 aactggttct ctatcacgta ctgaccgtat tgctaaatac aaccaattac tacgtatcga
     9961 agaccaactt ggtgaagttg cagaatacaa aggtttgaaa tctttctaca acttaaaaaa
    10021 caaataattc ttttctaatg aagatacatg atcgactatt gaaagatagt cgagtatgat
    10081 cacttagtta agtaatcgaa ctctctactt gctgaaatat cagcaggtag agagttttt
//
LOCUS       DACDYI010000094         9666 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.094, whole genome shotgun sequence.
ACCESSION   DACDYI010000094 DACDYI010000000
VERSION     DACDYI010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.094"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..650)
                     /locus_tag="IWM52_002581"
     CDS             complement(<1..650)
                     /locus_tag="IWM52_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9974769.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIG"
     gene            975..1391
                     /locus_tag="IWM52_002582"
     CDS             975..1391
                     /locus_tag="IWM52_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP9974770.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            1388..1858
                     /locus_tag="IWM52_002583"
     CDS             1388..1858
                     /locus_tag="IWM52_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9974771.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            1872..2645
                     /locus_tag="IWM52_002584"
     CDS             1872..2645
                     /locus_tag="IWM52_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9974772.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAAAPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            2659..3471
                     /locus_tag="IWM52_002585"
     CDS             2659..3471
                     /locus_tag="IWM52_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9974773.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            3525..4490
                     /locus_tag="IWM52_002586"
     CDS             3525..4490
                     /locus_tag="IWM52_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9974774.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            4606..7329
                     /locus_tag="IWM52_002587"
     CDS             4606..7329
                     /locus_tag="IWM52_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9974775.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELYITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESKILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGEAIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            7342..7974
                     /locus_tag="IWM52_002588"
     CDS             7342..7974
                     /locus_tag="IWM52_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9974776.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKDLRKEVKPLLDFLKKRDIP
                     LALASSSPVVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTVALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            8873..>9666
                     /locus_tag="IWM52_002589"
     CDS             8873..>9666
                     /locus_tag="IWM52_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9974777.1"
                     /translation="MKNLQLKFIVDPSIVRWFRIINQFERNQLATKGELAKSNHVSER
                     TIQTDVNKMKDYFSESVQFVSTKSGYSFQKERPVLFLKQKEQLLENEILFELLGNVFY
                     GELEDMTELIDRFHLSEATFRRYLKQIQPVLEEYGLELSLYPLDLKGEEANIRKFFKD
                     FYYEGEMTPHTLVPPRDLHELVQATFYDKFEYFSIGTGTSPAEFYYSLYIAIERVRQG
                     KTIAIPQGLIERVIASENFRLMYSLKEAIQTNYQVALSEEEFCWLY"
BASE COUNT         3260 a         1476 c         1885 g         3045 t
ORIGIN      
        1 ccaatcatat ggaagccacc gattaggtat tttatgggtt tagtcggaaa tgtttcaaga
       61 gcaatttcca tcatatcgat aatgccatga tgactacagc cagtaaagac aatcagaccg
      121 tcttcttgct caattacgac caataattca tctgaaaaat catcataaac aaatttatca
      181 tccatctttt tgtataaatt cttggtggtc gagtttgctt tgcctagggg gatgatatgg
      241 aagctcggag caagttctgt tttcgttttt attgtatgta aattctcatg cattttaagg
      301 atttcatttt taggaatacc aacatacaga aacgtggtta aaatctttaa ataatggggt
      361 aaagttacac cttcagctgt ataaataggg gcgtttcctg attttaaata tcttaacccg
      421 ccggtgtggt cagaatgacc gtgtgaaata atcactgcct ccagatcatt caaagtttcc
      481 cccattttag tcgcattata gactaatttt ttattagcgc cagcatcgaa gagatatttc
      541 ttccctgagt agttaattaa gagagataac ccatgtccac ttttaaagtt caaatttatt
      601 cttgagtttt caaataaaac cttgatttcc actataacat ctcctttcac atgaacgtgt
      661 attcatatat tgtagcacat taagttctat tttttgttgt aattaccgaa aaaaatatta
      721 atggacgctt tttacgcttt atttcgtatg tggaagaaaa tttcttagga tgtggcgggg
      781 gattggggca aagctaaatt ccaattacag tgatcattta attagacacc tttaaatacc
      841 cacactttca tcaacactat aatttaggga aagaaaagtg ttggcatgat atagaaaaac
      901 ctttaattaa cagcgtttcc taaaattggc acgcatcttg cttatagaaa agcgtaagga
      961 taggaggaat gaaaatggca actataattc ttgccagcca tggggaattt gctcaaggat
     1021 taaagcaaac agcgacaatg attattggag atagtgtacc aattcatgcc ttgtcagcat
     1081 ttagagatga agatgaatct attttggtac aaataaaaaa acttttagcc acaatagata
     1141 tagaagaaac ctacatttta actgatattc taggtggtag tgtgaataat gatatgctca
     1201 ctattattaa agagtatgag ggattgcaat taattactgg aatgaactta cctttagtta
     1261 tttctattgc aacctataat ggaaaaattg gggaaagtga gctagaaaag cttattaaag
     1321 agagtcaaca atcagtgatt gattgtagaa aattattagt agaacaactt actggaggag
     1381 atgacctatg attaagttag tacgtattga ccatcgtttg ttgcacggac aggtagtatt
     1441 ttcttggagt aaatcattaa atatcaatcg tattattatt gccaataaag aggcagcgaa
     1501 tgatgaattc aaaaaaatgt ctttgaattt atctaaaccg actggcatta aattaacaat
     1561 tttttcagtt gaacagttac tagaaaaaat gcctaaaatc gaaacactta acgaaaatat
     1621 catgcttatt tttggtaata caaaagaaat tcgtcaattt tgtgaagcct atcctaaagt
     1681 acaagaaatc aactatggag ggataatcaa aaaagaaggt tccaagcaat ttagtaatgc
     1741 cattttcttg aatgaatctg agcaagaaga tgccaaaaaa ttaaaagctc ttggcattaa
     1801 acaatttatt caacaggtac caacttctaa aaaagaagat ttgaatacaa tgttataaag
     1861 gaggaatgaa aatgctcgtt cagtcaattg gtttagggtt aatcggtgtt ttttgtattt
     1921 tagactcaag gcttttagga agaatgaatt ttgaacgacc gttgattgtc agtacgcttg
     1981 ttggtttggt acttggagac atggaaaaag gattaattgt tggagcctct ttggaattga
     2041 tgtccttagg attagtaaat atcggagctg ctgcaccacc tgatatgaat atggcatcaa
     2101 ttattgctac agcctttgcc attttatcaa atgcgaatgc agaagcagca ttaacgattg
     2161 ccattccaat ttcagtgtta ggtcaaatac taggtatctt aatgcggacg attttgtcaa
     2221 atttgactca cgttgcggat cgctttatcg aacaagggaa atttagccaa gcaaggagaa
     2281 ttcatatact ttatggctcg ggattatatg ctctcatgta ttttattcct atttttctag
     2341 caatttattt tggaacgaat cttgtttctc agattgttga tgcgattcct gagtggctaa
     2401 caaatggctt aacggttgca agtaaaattt taccagcata tgggtttgcg ttgttattac
     2461 aaacgatgtt aacgaaaaaa atggtgccat ttttattact tggattttta attactgctt
     2521 attctggatt gggaatcatt ggtattgcag ccttcgcatg tattgttgcg tttgtcatgg
     2581 ttcaagttca aggtaaaaac tcaaatggta accaaaatga gccagatcca ttagatgact
     2641 tataaggagg tagattaagt gaaagagaaa gatcaaaatg catggaaata tcggcagttc
     2701 ttatggcgtt catgggcaat tcaagcctca tggaactatg aacgacaaat gaacatgggt
     2761 tttatgtacg gaattgcacc aattctagat aacatttaca agaatccaga agatgaagca
     2821 atgaagaaag aagcttacga gcggcatatg atgtactaca actgtacccc tcaaacaagt
     2881 gcatttgtgt tagggttaac tgcttctatg gaggagcaat actacgaaga ccaagaaaac
     2941 tttaatccgg aatcaatcag tgcagtgaag acttctctga tgggaccgct atctggtgtt
     3001 ggtgattctt tctttcaagg aacgattcgg gtattagcct ttggactagg aattaattta
     3061 gcccaacaag gtagtatttt aggtccaatt ctagcaatgt taatttcctt tgtaccttcc
     3121 tttttagtta cttactatgg tgggaaaatt ggctataaca tggggaataa atacttgtct
     3181 aaactgtatc aagaaggttt gatggataaa gtaatgtatg tttgctccat tgtggggttg
     3241 atggtcattg gttcgatgat tgccagcatg atcgggatta caacaccaat tcaaatgggt
     3301 aaagcttttg tcttacaaga tgtattagat agtattatgc cacaattttt accattagga
     3361 ttaaccttct tcatgtactg gttattgcag aaaaaggtca aaacaggatg gatgttggca
     3421 atctgtttag tcggaggaat tatttttagt ttgttaggta tcttacaata agggaataac
     3481 caataaattt ggttgaataa tataaataag gaggaattca attgatgaat tcaaaagaaa
     3541 ttgttaaaga aatcgtagcg aaaaaagagg taaaatcaat cgtatttgtt ggctgtggcg
     3601 cttcaaaagc ggacctttat cctgcaaagt attttctaga ccaaaatgcg aagcagttac
     3661 ggattagtca ttacacagcc agcgaattta actatgcaac accagcatct gtagatgagt
     3721 caactgtcgt tatttcagct tctttaggag gtacgactcc agaaacagtt gaagctaacg
     3781 cagtagctaa agaaaaaggt gctacagtta ttagtctaac acgaagcatt gactcaccgt
     3841 taacaaaaga tgcagattat gtaatttatc atcgatttgc agagaactat gctgctaaat
     3901 tagaaaaaac aggctactgt ttagaattgg cagtagaact tttacatcaa gtagaaggtt
     3961 atgaccatta tgaagcaatg caagaaggat ttgctactat ctatgatctt gcccaaaaaa
     4021 gtgcagaatc agctcgtaaa gatgcgaaga aatttgctga acgctttaaa gatgaaaaag
     4081 ttatttatgt aatgagtagt ggtgctacac atgaagtagc atactcaaca tcaatttgct
     4141 tgatgatgga aatgcaatgg attaattctg gtaccttcca ttcaggagaa tttttccacg
     4201 gaccattcga aattgtggat aaagacgtac cgtttatatt attaatgaat gatggaaaaa
     4261 ctagaaatat agatgctcga gcattaactt tccttgaacg ctttgatacg aaaacagaag
     4321 ttgtagatgc cttggattat ggcttgtctt cacatattgc taaagaagtc ttagactact
     4381 ttaacccatt cgtgattact gcagtattta gagtttatgc agaagaattg tcagaagcaa
     4441 gagaacatcc attaactaag agacgttata tgtggaaact aacttattaa aaaatagatc
     4501 aaactaaata aggtggcttg agaaattaag ccaccttgtt ttcttcaatg tgattttata
     4561 catatggtaa aataactagt acatattgtg aaagcaggtg gcaagatgca gcgattaaat
     4621 gactatcttt atcaactaac attgcttttg aaatctacgc cttcagataa ttattcagct
     4681 cgcagtattt cgcaagcttt acagttgaat agaagtacaa tcagtagcta cttaaatgaa
     4741 ggtgttcgtg aagggctttt cataaaagta aaaagttacc cggttttatt tttacataga
     4801 acagctcttg aagaactata tatcacactg aataactccg aaatagaatc aattgaaagc
     4861 ttgttaacaa tttctcaaaa acccgcatta gatcaagtaa ttggctctaa aggaagtttg
     4921 aaagaagcaa ttgatcaaat taaaacggca gtcttatatc ctggaaaagg gttgccttta
     4981 ttattgatcg gcgctagtgg atcagggaaa acctttctag cgaataaaat atatgaatat
     5041 gcagtagaag aaaaggtcat tcacacatcg gcacctttca tagattataa ttgtgcacaa
     5101 tatgtgagca atccagagtt actatcctct gctttatttg gctacacgaa aggagcattt
     5161 accggagcaa gccaggagca tactggtttg ctagaaaaag ccgatggagg agtattgttt
     5221 ttagatgaag tgcatcgctt atctgaagaa gggcaagaga aattatttac atttatggat
     5281 acgggtgaat tctctcctat gggggataat agtattcgga aaaaagccga tgttcgatta
     5341 gtctttgcaa caacagaaaa tatatatacg actttcttac caacttttct taggagattg
     5401 ccagttattg ttaatctacc tagatttcaa caacgtccgt cgtttgaacg tctgtcattg
     5461 attgatgaat tttttgtttc tgagagtaaa attttagcta aggaactatc tgtttctgat
     5521 gcattgattc atttcttaat gaatagtgat tttgaaggta acgtggggaa tattaaaaat
     5581 attatcaagt atgcttgcgg aaacgcatat atccaccaga agagtcagca aacaattcaa
     5641 gttaaattat tggatatgcc tttagagtac agttataaat tcaaggagca actaaataaa
     5701 cccagaaaga aaagagcaga tcgaatctac tatccagaag aaacacgaca agtgcaacta
     5761 gaaagaaaca atcagctttt tgttcaattt tttgaccaat tacttactgg attctctgaa
     5821 gtgattgaaa ataacaagga tattaaacta tttttagaag aaatggttac caaggtaaca
     5881 caagtaatgg atacgcttat ttttcaagag gattacgaaa aagagcagtc tttatacgcc
     5941 attctaaact atcatattcg tcaaagtatt gattttatgc gagaaagcta cgggtttgaa
     6001 caagatggta accgggtagt cgctttggcc aactatctat atgtcaagga taatcaagtg
     6061 atttttgaaa atagtcttca agaaaagaaa accaaagatc agcttaacga attttttgct
     6121 aatgttcttg aaacccctta ttggtatgca aaaaaaatct taagcctttt atctgagtgt
     6181 ttagatcaac ctgtgtatga agaggacatt ttctttatta cgctatattt ttatagtttg
     6241 aatgtcacga atgttaaaac aggtattaag agtattgtgc tagctcatgg ttattctaca
     6301 gctagtagta tggcgaatgt tgccaaccgt atgctgaata agaacgtttt tcaagcttat
     6361 gatatgcctt tagatattac attagagaaa attgaagcaa aattgattca attccttgga
     6421 gattatcgaa cagattcagg cttgatttta ttggtagata tgggttcact aaaccaacta
     6481 ggcggtttat tagtgaatca tctaaatgga ccactgttaa tcattgatca tatcaataca
     6541 ccgttattat tggaagtcgg ccaacaagta ttaaacaata aaagtatcac cgaaatagga
     6601 gcagaaattg aaacaagtct tactttcaaa aagcagttac tacttcccaa gaaaaagaaa
     6661 aagaaagcca tcattacttg ttgttataca gggattggta gtgcgaccca gattcaggag
     6721 attttaacga aatgcttgaa agatgcagtt agagaattag taattattcc gtatgattac
     6781 aaaaagctgt tagaaaacaa gaattttgag actccttttc aattatacga tgtcatcatg
     6841 attgtaggta cagaagatcc caaaattaat cagattccat atattggatt agatcgcctg
     6901 attaatgggg aagcaattgg cgaatttgtt cattacttgg aaaaagagct gactattgaa
     6961 gtgaacgatt tacaaaaaga attaattttc aatttttcaa ttagtaaaat tgtcgaaaat
     7021 ttaaccattt tggatccaga taaggtgcta tcttttgtac aaaaagcaat taaagaatta
     7081 tcgaagttat taaattttga tttttctaat aatcagctgt ttttgttgta ccttcactgt
     7141 agttgtatga ttgaacgaat tgtacgtaaa gaaagtgtgg atgagcaaga ggacattgaa
     7201 gaatatatgc aagcaaatgg agaaataatg gataaaattc atagcgcttt ttttgagatt
     7261 gaaagggaat acacaattga aataccagat ttagagttgc gattattgag cgatattgtt
     7321 caaggatagg agtgaattcg aatgacaatg ggagtaattt ttgatatgga tggggtactc
     7381 atagacagcg agacatttta ttttgagaga agaatgaact tctttcaaga actggagata
     7441 gatccaggat caaaccagat tgatgattat gtggggaaaa cagaagctgc aatatgggaa
     7501 acattagttc ctgaggacaa atcattaaga gaggaacttt atccgaaata tgtagattat
     7561 cgaaaaacgc atccaattga tttcgctaaa gatttacgaa aagaagtgaa accgttactt
     7621 gacttcctca agaagcggga tataccattg gcactggcat cttcttctcc agttgttgag
     7681 atagagagta tgctcattca atgtgaactt actgaatatt tttcctttgt tataagtggt
     7741 gagtcattaa aacaaagcaa accacatccc gaaatatatt taaaagcgaa acagcaatta
     7801 gactgtgact tctttattgc agtagaggat tcaacgattg gtattaaatc agcaaaggca
     7861 gcaaatatct atacggtggc actaaaacaa ccaatgcaaa tcgatcagag tgaagcagac
     7921 atacaaattg aaagtctaca agaactggaa caaattgttt tagaaaaagg ttagtgaatt
     7981 aatggaaata aggctatata tctgtgctag ctattttgac taaccaaaaa aacttgctat
     8041 aattcaggtg tagtaaaagg tgctggtaac accttttacg aaaatgcttc ggcagtgata
     8101 atgggattgg ctttttaagt tttttatttt gttcgcaatc cctaaggtca tgtgaatgat
     8161 cctagggatt gctagaatat tttgcaacat tctatctatt tcagaacgta accttcccca
     8221 gttcattggg attcattagc atacaaccaa catttcaaaa agaaattgca ctacaccatt
     8281 attagctatt ttgactaacc ccaaaaaact tgctataatt caggtgtagg taaaaggtgc
     8341 tggtatcacc ttttacgaaa ctgcttcggc agtggtaatg agtgtggctt aaaggactag
     8401 tcctttaagc tttttacttt gttcgcaatc tctaagatca agttaatgat cgataagatt
     8461 gctagaatat tttgcaacat tctatttcag aacgtaacct ttcccattcc actgtgattc
     8521 attggcaacc ctcctcacaa ccaacgtttc aaaagaaatt gtaaagttgt tggggtacca
     8581 cgaccgaaca atactacacc tgaatgatag taagttgtct cattaagtat agcatatcct
     8641 ctttttgggt aaatagaaat cggttacatt ttttatccaa gaccaagttt tttcatttgg
     8701 tcttatttgc tatacccaaa caattccgca gtatagtcga cggtattgat tttatcaggt
     8761 gagtaataga aacttgtttt ccttccccaa aacattacct ataaaggaga aaaaatggta
     8821 tcgtaattat ataggtatag gatgaaattt ttttctaaga ggagggttgt tgatgaaaaa
     8881 tttacagtta aaatttattg ttgatccatc gattgtacgc tggtttcgga ttatcaatca
     8941 atttgagcgc aatcaacttg ccacaaaagg agaattagca aagagtaatc atgtatctga
     9001 acgaaccatt caaacagatg tcaataaaat gaaggactac ttttctgaaa gtgttcaatt
     9061 tgtttcaacg aaaagtgggt attcttttca gaaggaaaga ccggttcttt ttctcaagca
     9121 gaaggagcaa ctactggaga acgaaatttt atttgagttg ttagggaatg tcttttatgg
     9181 cgaattggaa gatatgacgg agctaattga tcgttttcat ttatctgaag cgacttttcg
     9241 tcggtatttg aagcagattc aaccagtttt ggaagaatac ggcttagaat tgagtcttta
     9301 tcctcttgat ttaaaaggag aagaggcaaa tattcgcaag ttttttaaag acttctatta
     9361 tgaaggggag atgacgcccc atactcttgt tccaccaagg gatcttcatg aacttgtgca
     9421 agctacattc tatgataaat ttgagtattt ctcgattgga acagggactt ctcctgctga
     9481 attttattat agtttgtata ttgcgattga gcgtgtgaga caaggaaaaa cgatagccat
     9541 tcctcaaggt ctgattgagc gagtaattgc tagtgagaat tttcgcttaa tgtattcctt
     9601 gaaagaagcg attcaaacaa attatcaagt tgctttatca gaagaggagt tttgttggtt
     9661 gtattt
//
LOCUS       DACDYI010000095         9285 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.095, whole genome shotgun sequence.
ACCESSION   DACDYI010000095 DACDYI010000000
VERSION     DACDYI010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.095"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            113..805
                     /locus_tag="IWM52_002590"
     CDS             113..805
                     /locus_tag="IWM52_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP9974778.1"
                     /translation="MRKKIIINAGVIIGLMLVIFSGKQYADNILLWGGEDNIEKINNN
                     LTILDTALENKEQKISILNSQLSSNNQTLEQLRTNIEVSKQKVASLENDKNQLVSEKT
                     NLENQLSAKDSELQGKQNEINAKIDEINQKVAEINRLNSDWNNRLQNEKNKFNEEQST
                     INSLNQQINQMLSEKNNIQNQLQATQQKLEEEKSKNSSLNNYIEKLQKAKNDVEDTAN
                     KAQQIVNEHTIQ"
     gene            1233..1625
                     /locus_tag="IWM52_002591"
     CDS             1233..1625
                     /locus_tag="IWM52_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974779.1"
                     /translation="MLDDRLTCSNCGTLFYYEEVRNIVKHRKKEMPIRCPNCNEVIKR
                     KLSHGYFVSYELYEESKINLKKKPKYYKNLKTGAIITEKEFKNLVTEEESRFYNLSGN
                     ICPGFMDQIIENESIESIENYVPVDLPK"
     gene            complement(1798..1980)
                     /locus_tag="IWM52_002592"
                     /pseudo
     CDS             complement(1798..1980)
                     /locus_tag="IWM52_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            1967..2164
                     /locus_tag="IWM52_002593"
     CDS             1967..2164
                     /locus_tag="IWM52_002593"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974780.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            complement(2207..2782)
                     /locus_tag="IWM52_002594"
     CDS             complement(2207..2782)
                     /locus_tag="IWM52_002594"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP9974781.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(2937..3463)
                     /locus_tag="IWM52_002595"
                     /pseudo
     CDS             complement(2937..3463)
                     /locus_tag="IWM52_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            3920..5431
                     /gene="gadC"
                     /locus_tag="IWM52_002596"
     CDS             3920..5431
                     /gene="gadC"
                     /locus_tag="IWM52_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HAP9974782.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            5444..6844
                     /locus_tag="IWM52_002597"
     CDS             5444..6844
                     /locus_tag="IWM52_002597"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003134257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAP9974783.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(7009..8445)
                     /locus_tag="IWM52_002598"
     CDS             complement(7009..8445)
                     /locus_tag="IWM52_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9974784.1"
                     /translation="MLETYLEKSIFRQVYICEQLHEKGTIQIREIADQLNVCPLTVTN
                     DLECITDILHQQIISVEKSRSSFTVAFDSSFSRLALTQLIYQRSDFFNVLGHYLSGEH
                     NWSNIADTTFISLSKVYNLRNELINFFKQMQYLNADGHFEIPEKDYRSLLLTIIYETN
                     RTELIQLNKSIIQSGQQLIDYVEKHFFSRSYPTVEKQFILLGIAIGLQRSKTNPIYFS
                     QEEKRVARHTPLFQLIQKGLEQLSFSLCHDEDECLYIYFLFNSRNYLCDNFELLQKDL
                     NVVYQNHVADNPLVIELSQKLILSLNISEENQILFDKALLPFIRSLWADIQIFQPDKT
                     YLLNEEQRALYLEVHEILEQWRKQNNLFLRWNENSIRKLTISLSLLNEHKRKSPIEVF
                     IVAPSDFRYLYYRQQLEDILGEHFSISNIICKQLREIVDDTFFCTQRIILCDSSLYQE
                     GLGSEKTIIYPITFQTIHTVIDQLNKQI"
     gene            8838..>9285
                     /locus_tag="IWM52_002599"
     CDS             8838..>9285
                     /locus_tag="IWM52_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HAP9974785.1"
                     /translation="MNKDITFPVMDVRLVPITKIQANDYNPNRVASPEMNLLELSIIE
                     DGFTQPLVCYYKEKEEKYILVDVLHRYRVAKERLNLKVVPVTVIDKPIEERMASTIRH
                     NRARGTHEIRGMEIVIQRLVKNGWSDKRISKELGMDIEEIFRFKQVS"
BASE COUNT         3104 a         1464 c         1630 g         3087 t
ORIGIN      
        1 cctttttaaa taatttttta gacaaaaaac agaaagtgtc taaagaaagc ttgacgaaga
       61 aaataatttt taataactta tttaaggaaa ttttttgaag gagaagataa aagtgagaaa
      121 aaagattatt attaatgctg gagtaattat aggattgatg ttagtaattt tctccggaaa
      181 gcaatatgct gataatattt tattatgggg aggggaagat aatattgaaa agatcaacaa
      241 caatttaact atattagaca cagctctaga aaataaagag caaaaaatta gtattttaaa
      301 ttcacaattg tcttcaaaca accaaactct tgaacaacta agaactaata ttgaagtttc
      361 caaacaaaaa gtggcatctt tagaaaacga caagaatcaa ttagtatcag agaaaactaa
      421 tttagaaaat caattgtcag caaaagactc tgaattgcaa ggaaaacaaa atgaaattaa
      481 tgcgaaaata gacgaaatta accaaaaagt agctgaaata aatcgattaa atagcgattg
      541 gaataatcgt ttacaaaatg aaaagaacaa atttaatgaa gaacaatcta ctattaatag
      601 tttaaaccag caaattaatc aaatgttgag tgaaaaaaat aacattcaaa atcaactgca
      661 agctactcag caaaagctag aagaagaaaa gtcaaaaaat agtagtttga ataattacat
      721 tgaaaagctt caaaaagcta aaaatgatgt agaggatacc gcaaataaag cacaacaaat
      781 tgtaaatgaa catacaatcc aatagatata gttttaattt aacaaatggt atgtttggag
      841 ataatgagat agaatataaa tgtcataata ttatacaatc taaacaccgt ttccttttct
      901 atttttgatg acatcagtat atagctactc gaaagagtag tctttttatt attctcgctt
      961 attattgata tgaaaatttc gatttgacaa aaggtaccga ataattttgt aatatgaatt
     1021 attatcaaga agtaattatg atgataacat atggaaacta gttagtaata ataacaggtg
     1081 attaaacttt tgaacgactt gtaattctat attttttctt ttaagtaaaa aatataggtg
     1141 attttttttg acttcatatt tataataaag acacgtaaat caattatttt tcattttact
     1201 ttaactaata atgtttattt ggagggatta ttatgttaga tgatagattg acgtgttcta
     1261 attgtggtac attattttac tatgaggaag ttagaaatat tgtaaaacat agaaaaaaag
     1321 aaatgcctat acgttgtcca aattgcaatg aagttattaa gcgaaagttg tctcatggtt
     1381 attttgtcag ctacgaacta tatgaagaaa gtaaaataaa tctgaaaaaa aaaccaaaat
     1441 actataaaaa tttgaaaact ggagctatta taactgaaaa agaatttaaa aatttagtga
     1501 ctgaagaaga aagcaggttt tataatttgt ctggtaatat ttgccctgga tttatggatc
     1561 agattattga aaatgagtca attgaatcaa ttgaaaacta tgttcctgtt gacttaccaa
     1621 aatagaaaaa ataatcctgt ttttacagtg aattatttta attttgattt tcaataaaaa
     1681 tatattacaa taataatcgt aatagctaat gaaacaattt ctcaatcttt ttaccactcc
     1741 aaacagggag cgtcaataat tttgtgtaaa taactcgtcc ttctgcaaaa taatgggtta
     1801 ctcagtaaac attgaagcta atgtatccgt cacttgttgg aagcctttat ggcttctatt
     1861 taagaatttt tgattgtatg tatcaaagat ggatactagg aaacgctcta gtgattcttc
     1921 attttgaaac tgctcttttc tgcggctgta tttcttgatt tgtttattga aagattcgat
     1981 tgtagaacca gaaggaatgg agaacacttt tgaactttac aacaaaacta ctgttgataa
     2041 attaattcca attttagttc caactgattt aaatggtcat gatgttttac ttcgttttaa
     2101 cggattttta ggtgaggatt atagatacct aaaaatttct gatcctcaac ctttaacaga
     2161 ataataaaaa acgtgtaatc aattatttga ttacacgttt tttatttcaa tcttcgttgt
     2221 agtagtggtt gcgacatacc gaaaggtaat cttcattacc tcctactaca atctgttcac
     2281 ctgaataaac tggaatacca tcaacaatac gagcattcat tacagccttt ttattgcaaa
     2341 aatggcaaat agttttaatt tcctcaattt tatcagcaaa tgtcaataat gcttcggatc
     2401 ccttgaagag attattttga aaatcattct tcaatccata gcaaattact ggtacttcct
     2461 cattcattac gataagtgat agatcaataa tattctctct acttaaaaat tgagactcat
     2521 cgaccaaaat acaatcaact tttccgtact gatcaataat ctccactaat tctccgtaca
     2581 catccatttc agaagtaatt gcaatagctg gtttcgaaac acctatacga ctctcaactt
     2641 ttccaaatcc tgatcgagta tctattgcgc ttgtcattat gataactctc ttcccttgtt
     2701 cttcataatt atgagccact ttcagtagct caatactctt tccactattc attgttccat
     2761 atctgtaaaa taattgtgcc attaaattta ttcccttctt ttacaattca attattcctt
     2821 aattccatct gcttttatgc agctgctcgt ttattccttg atatgtctat aacatctact
     2881 atccctaaaa ccaatttata agccattaga gatggtattc ctgaacatct ctcatattgt
     2941 tttttgagtg gtatatcatc tgaaaaactg aaggaatcat gaaaatagcg acatacttat
     3001 catttagagt tctaacttta aatgcattta cttcttcagt cagcacttta gtcatgttgg
     3061 acatggtttg gggcgtgtag tgatgcccat acattttctc aatcaagtct gcgatttccg
     3121 acatggtaat accttttcgg aagaggtgaa tgacggtctc ctctaatgtg tcattcgtcc
     3181 gtctataagc aggaacagtc tgttgcttga actcgccatt gcggtcgcgc ggaatctgga
     3241 gatgaagttc cccatactcg gtcttgaccg tacggtcata ggagccgtta cgagaattac
     3301 ccgtgttaaa accaatgcga tcgtactttt cgtaatcgag gaaagccgtt aactccgttt
     3361 tgagaagcgt attgacagct ttttccaaat ggacacggaa taatccattc aaatcgcctt
     3421 tgttcgctag agtctttagg atttctgtag taaaatcgtt catatggaag tcctcttttc
     3481 tgtgaatgtg ttgtggtaac tctattctac agaagggact tccttttttc tatttacaca
     3541 aaatatttta cactctcaac aaaagtatct tcgattaata cttgctcaag ttatgtgcag
     3601 ctacgaaaaa gtaggtaccg aggtactttc tttttcgcag ctttttttgg acttatgaga
     3661 agtacattcg ctggaagaat acgatataaa acgagctttt tcactaaaga tattaataga
     3721 gtgaaagaaa caagcttttg ttgtcagttt tttcactttt ctaaaaatga aaataaaaaa
     3781 ttctggaagt ggagaagaat tttgcttgct atactttgtc ttgtagaaaa gaggtagaca
     3841 aaataatgca agctcaaata agcgattttc cataaccttg gtaggaatga caaattaaat
     3901 aggaaaggga agtaataata tgaataaaaa aaagttatct ctattcggtt tttttgccct
     3961 gacagcttcc atggttttga ctgtttatga gtatccgacc tttgcaacat cacaattaca
     4021 tttggttttc tttcttcttt tgggaggtat tttatggttt ctaccagttg ctttatgtgc
     4081 tgcagaaatg gcaacagttg aaggatggaa agatggtggg attttcagtt gggttagtca
     4141 aactttagga gaacgttttg gttttgcagc tatttttttc caatggtttc aaattacagt
     4201 aggttttatt acaatgatct acttcatact aggagcgctg tcgtatgtaa ttaattttcc
     4261 agagcttaat acagatccat ttatcaagtt tattggtgta ttagtcattt tctgggtact
     4321 gactttttca cagttgggcg ggaccaatcg taccgctaaa attgctaaag caggatttgt
     4381 cattggaatt gttattcctt cgatcattct ctttggttta gcagcagcct actttatcgg
     4441 cggtaatccg attcaaatgg ctattaatgc gaaagctttt attcctgact tttctcagct
     4501 ttcaacttta gtagtatttg tatcttttat attggcttat atgggcgtag aagcttcagc
     4561 atcacatatt aatgagctgg aaaatcccca aagaaactac ccattggcaa tgtttttact
     4621 agtgattttg gccatcgcct tagatgcaat cggcggtttc tcagtagcag cggttattcc
     4681 acaacatgac ttgtctctca gtgctggtgt aatccaaaca tttgaaacgc ttattttaca
     4741 ttttaacagt catttacatt ggttggtaaa agtgattgct ctcatgattg cctgtggcgt
     4801 tatgggtgaa gttagttctt gggtagttgg tccatcacga gggatgtttg cagctgctca
     4861 gcgaggtctg ttaccagttt tcttccggaa aactaataaa caagacgttc cagttccgtt
     4921 gattatgata caaggaatta tcgttactat ttgggcagca gtcttaactt ttggtggagg
     4981 aggcaacaat ttatcattct tagtagcaat ctccttaact gttgtcattt atctcgtagg
     5041 atatttacta tttttcctag gttactttgt attagttttt aagaaaaaag acttaaaacg
     5101 aacttacaat gtaccaggaa aaactgttgg caaaacgatt atcggtggaa ttggttttat
     5161 tttatcagtg ttcgcattgc ttatctcttt tgtaccacca gcttcaattg cagctagtga
     5221 gcaccgaaca taccaaatca tactacttat tagctttgta gtgactgttc ttttaccttt
     5281 tatcatttat gaagtacatg ataaaagtgc tcacacaact attgaggaac ctactcacgt
     5341 gaaagctgaa gacgttaatc ccgctgttta tccagctgct agaggagagc accacgttac
     5401 aaaaaatgat gaacatattt taaaacatta ggaggaattc attatgttat acggaaaaga
     5461 taatcaagaa gaaaaaaact atttggaacc aatttttggc tctgcaagtg aggatgttga
     5521 cttgcctaaa tataagttaa acaaagaatc cattgaacca cgaattgctt atcaattagt
     5581 acaagacgag atgttggatg aaggaaatgc gcgattaaac ttagctactt tttgtcaaac
     5641 gtatatggaa cctgaagcag tgaaattgat gacccaaacg ttagaaaaaa atgcaattga
     5701 taaatcagaa tacccacgaa caacggaaat tgaaaaccgc tgtgtaaata tgattgctga
     5761 tttatggcat gctccaaata atgaaaaatt catgggaact tcaacgatcg gctcttcaga
     5821 agcctgcatg ctgggtggta tggccatgaa atttgcttgg cgtaaacgtg ctgaaaaatt
     5881 aggtcttgat attcaagcaa aaaaacctaa cctggtgatc tcttctggtt accaagtttg
     5941 ttgggaaaaa ttctgtgtat attgggatgt ggaactgaga gaagtcccaa tggatgaaaa
     6001 acatatgtca attaatctag atactgtcat ggattatgtg gatgagtaca caattggtat
     6061 tgtaggtatt atgggtatta cttacactgg tcgttatgat gatatcaagg gtctgaatga
     6121 tttagttgaa gctcacaata aacaaactga ctataaagta tacattcatg ttgacgctgc
     6181 atcgggtggc ttttatgcac catttactga acctgatcta gtttgggatt ttcaattgaa
     6241 aaatgttatc tcaattaatt cttcaggtca caaatatggt ttggtatatc caggtgtggg
     6301 ttgggtctta tggcgtgacc aacaatactt accagaagaa ttagtattta aagttagtta
     6361 cttaggtgga gaaatgccaa ctatggctat caacttctct catagtgcag cacaactaat
     6421 tgggcaatac tacaactttg tacgctatgg ctttgatggt tatcgtgata ttcaccaaag
     6481 aactcatgat gttgctgttt atttagccaa agagatcgaa aaaactggta tttttgaaat
     6541 cattaatgat ggatcagaat tgccagttgt gtgctataag ctgaaagaag atcccaatcg
     6601 cgaatggaca ctatatgatt tatctgatcg tctgttaatg aagggatggc aagtcccagc
     6661 ctacccatta cctaaagact tggatcaatt aattattcaa cgcttagttg ttcgagcaga
     6721 ctttggaatg aacatggctg gtgattatgt acaagatatg aaccaagcaa ttgaagagtt
     6781 gaataaagct catattgttt atcataaaaa acaggatgtg aaaacgtacg gctttactca
     6841 ctaaaattct tcagagaaat ataaaagaag ttaattaact aataaaagga gttgtgatta
     6901 ccacacagct ccttaaatct atttgaataa atatatgata ttcatttgtt acactctcaa
     6961 tcttagctgt tgttctattc atcattactc ttattgtaag tagttcagtc aaatttgctt
     7021 gttcaattga tcgataacag tatgaattgt ttgaaaagta atcggatata taattgtttt
     7081 ctcactacct aagccttctt ggtataaaga actatcgcaa agtataatcc gctgagtaca
     7141 aaagaaagtg tcgtctacta tttctctaag ttgtttacaa atgatattac taatagaaaa
     7201 gtgttctccc aaaatgtctt ctaactgttg tcgatagtat aaatacctaa agtctgacgg
     7261 agctactatg aacacttcaa ttggagattt ccgtttatgc tcatttaaaa gacttaaact
     7321 aatggttagt ttacgaatag aattctcatt ccaacgcaga aataaattgt tttgttttcg
     7381 ccattgttca agaatttcat gaacttctaa gtacaaagcg cgttgctctt catttaacaa
     7441 gtaagtctta tcaggttgaa atatctgaat atcagcccaa agtgagcgga taaacgggag
     7501 aagtgcttta tcaaaaagta tctgattttc ttcagatata ttcagagata agataagttt
     7561 ttgactgagc tcaatcacca aagggttatc tgctacatgg ttttgataaa caacgttcaa
     7621 gtctttttgt aacaattcaa agttatcgca tagataattt ctagagttga atagaaagta
     7681 gatataaagg cattcatctt cgtcatgaca taaagagaaa gaaagttgct ctaatccttt
     7741 ctgtattagc tgaaacaagg gagtatgtcg ggcaactctt ttttcttcct gactaaaata
     7801 tattgggtta gtcttagatc tctgcaaacc gatagcgata cctaataaaa taaattgttt
     7861 ttcaactgta gggtaacttc ttgaaaaaaa atgtttttct acataatcaa tcaattgttg
     7921 accgctttga ataattgatt tatttagctg gatcaactct gtccgatttg tctcatagat
     7981 tatcgttagt aacaaacttc gataatcctt ttcaggaatt tcaaaatggc catcagcatt
     8041 taagtactgc atttgtttaa aaaaattaat caactcattt cgtaagttgt aaactttgct
     8101 taacgaaatg aaggtagtat cagctatatt actccaattg tgttcccctg ataaataatg
     8161 tcctagtaca ttgaaaaagt cagagcgctg atagatgagt tgagtgagtg ccaacctcga
     8221 aaacgaagag tcgaatgcga ctgtaaagct acttctagat ttctccactg aaataatttg
     8281 ctgatggagt atgtcggtaa tgcattccaa gtcgttagtg acagtgagtg ggcaaacgtt
     8341 caattgatct gctatttcgc gaatctgaat ggtccctttt tcgtgtaatt gctcacaaat
     8401 gtaaacttga cgaaaaatac ttttctccag ataggtttcc aacatagtac tcctcctagt
     8461 cattttatac ataactatta taactctacc aatcggaata gtcctgtaat atattgaatc
     8521 aaaaatataa attataaaat aattagatgg ggtatttggt ttaagtatcc cttatatagt
     8581 aataaattta taggtgtgat tctcgaatta atacccgaaa atgtgagaga atttttatgg
     8641 attttttagg atacaaatta attatttttg cttttatcct gattaccagt ccagtactgt
     8701 cggtatccca ttttgcagca aaatcatgtt gataaagtga caagaagact gatgagatat
     8761 attatccaaa ttgagaagat aattaatcgg attcttgagt ataccctact ttttaagaaa
     8821 gtagggtata caagcgtatg aataaagaca taactttccc tgtgatggac gttcggcttg
     8881 ttccaattac caaaatacag gcgaacgatt ataatcccaa tcgagtagcg tctccggaaa
     8941 tgaacttatt ggagctttct atcattgaag atggctttac tcaaccatta gtttgttatt
     9001 acaaggaaaa agaagagaag tatatcttgg ttgatgtgct tcataggtat cgtgttgcta
     9061 aggaaagact caacttaaaa gttgtaccgg taacagttat tgataaaccc atagaagaac
     9121 gtatggcttc aacaattcga cacaatcgtg caagagggac ccatgaaata agaggaatgg
     9181 aaattgtcat ccaaagatta gtaaaaaatg gatggtcaga caagagaata tccaaagaat
     9241 taggcatgga cattgaagaa atttttcgct ttaagcaagt gtctg
//
LOCUS       DACDYI010000096         9232 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.096, whole genome shotgun sequence.
ACCESSION   DACDYI010000096 DACDYI010000000
VERSION     DACDYI010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.096"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..132
                     /locus_tag="IWM52_002600"
     CDS             <1..132
                     /locus_tag="IWM52_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HAP9974786.1"
                     /translation="DGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVVQGELQ"
     gene            185..2161
                     /locus_tag="IWM52_002601"
     CDS             185..2161
                     /locus_tag="IWM52_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP9974787.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYRAYAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAVKNAEGKYYGGLVDTDKDGVKEAVWVDNVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPENYQKLTEDIAFKVNKNSFKEENRITVKNNQKAAI
                     PLTGSNGFQTYVLISCLLLGAGGLSAVVYFKKKA"
     gene            2174..2911
                     /locus_tag="IWM52_002602"
     CDS             2174..2911
                     /locus_tag="IWM52_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9974788.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFAFLLFLFIKK"
     gene            2925..3749
                     /locus_tag="IWM52_002603"
     CDS             2925..3749
                     /locus_tag="IWM52_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974789.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQPVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            4038..6149
                     /locus_tag="IWM52_002604"
     CDS             4038..6149
                     /locus_tag="IWM52_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP9974790.1"
                     /translation="MEGIMENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQ
                     IKENNNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAH
                     YQKVGTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQ
                     SINVMNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQ
                     QRVSWYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYQAPALSTNNLTSK
                     VVGGRAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTAL
                     LWTPQPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKS
                     FKITDTFDNKNFDMVSDGEKYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSF
                     LANAANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPN
                     KKVEQDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIALDHYKQFSIQD
                     PVEARLTYKEGSARVIDMSTGKDITSEGTLTYDSHSRTLKWEASADFLNNNLLDGREI
                     QLIFTAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLT
                     VIGSILFLTLASLGYVVLKLK"
     gene            complement(6187..8253)
                     /locus_tag="IWM52_002605"
     CDS             complement(6187..8253)
                     /locus_tag="IWM52_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAP9974791.1"
                     /translation="MKLLNQKTVIAGTFAVTLAATLALAGSVDAASTNVMYRLYNPNN
                     NEHFYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEV
                     KWLTKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWK
                     YEGIGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQNALNNAKKVLNDS
                     KATQKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQ
                     TALSNAKKVLDDSKASQAQVNNAKNSLDAAFKGLQHKPETPKEYMVTINHIDNQTGQV
                     IATDQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQV
                     HQINLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLN
                     NVTESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFL
                     CRNDDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQN
                     GVAPMQFNDIVQQAADIRAKELQVSFSHYRPDGGTFQDLLESLGCYGAKGENISQTGL
                     YIDDLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFIS
                     ANPIFK"
     gene            complement(8393..8599)
                     /locus_tag="IWM52_002606"
     CDS             complement(8393..8599)
                     /locus_tag="IWM52_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974792.1"
                     /translation="MYADKNEIEWLLFESGISVKDIYKESGVARTTVIDLINKTSSID
                     KMRFDNAVKLTKMAKMRKNLYENK"
     gene            8787..>9232
                     /locus_tag="IWM52_002607"
     CDS             8787..>9232
                     /locus_tag="IWM52_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974793.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLVNNLKIIDDTTWEYSESVWHPDPVQISVKRQKDYKGLERYKIVDSAGISDFI
                     NKPDSLFIVVPYEKDGVKKMVFLGSSKDEKRTKEKLIHDGSQSNKY"
BASE COUNT         3196 a         1462 c         1798 g         2776 t
ORIGIN      
        1 gatggcgttc ctgaagagaa ctctttcttg tctttaccga agaaaggaga gaaaccctta
       61 ttaacgttgt ttacttgtgt ttatacgtca caaggaaaag aaagatacgt ggtacaggga
      121 gagctacaat aacaaggttg taatcaacgc gagtcgttga aatatgaaat gaggagagta
      181 aaaaatggca ataaatcgat atgtaaaaaa agctggaatg cttgttggaa tgttaggaat
      241 cattgcaggc tgctttcagg gatatcgagc atatgcagaa gatgtcacgc aaaaaacgcc
      301 accagagaag gtcaatatca cggttcataa attaatgtac gatcaaggaa cgcaattgaa
      361 tgttgatatt gacgggatca aaaatgatgg ttatacgcac gatcaatatc ctacaggtgt
      421 aacgaaatac aacaaggcag attatggtga tgttgagttt acgttaggaa atattacgga
      481 tcaagtatta ccgagggaag attctgatct aaccaacgcg aaagtggacg aaatcgttaa
      541 agatgtagaa gacaagggat ctaacagtga gtacgttaag aatgcaacca acaaaattac
      601 ttcagctgta gatgaaaatg gtgagatcac ttttactgat caaccagcct atgtcaattc
      661 caaagggaat gtatatgtgg tttatgaaag taaatccgct gctggattag tcacacaaaa
      721 agcaaaacca atggttgtga ttgcgccaat gacagataaa acaggatcag gatttttaaa
      781 aaatattcat ttgtatccta agaacattgt ttctaagttg agttttgaat taacgaaatt
      841 tggtgacgat ggcgcagacc aaagcaaaca aaccctttta aaaggggcaa aatttgagtt
      901 atacaagggc gaacctggaa aaggaacaaa attgggtgat cttgtatcgg atgatcaagg
      961 aaagctgact gcgactgatt tgacgttagg aaaatactat tttgtggaag ttccttcaga
     1021 agtcgtagtg ggatcagatc aagaaccaac agcagatcaa tatttattag gcgcagacgc
     1081 tcgcaacgat gcgcacaata aattaacctt tgaaattacg aatgatggcg tgacaagtga
     1141 cttaaaagct tcatacgtaa actataaagc accagtgatc gataaaacag taacaaatgg
     1201 aacaggacaa gaacattctt tccaaattgg tgacgcagta aattatcaag gaacgattca
     1261 tattccaaca gacattgcag gtggcgcaga cggtatcaca gtaaacggtg tgaaaagcga
     1321 aacttctcct tacagtgtat ttaaatgggg agatacagca ggacaaggac tttcttatgt
     1381 cgctgctaaa gcaaatatca aagtaacaaa taaagatgga tctgtggtat taaaagaaaa
     1441 tacagattat aaaatccaaa attcagaaaa tggctttgtg atcgatttta tcgtgaataa
     1501 tggccaagta agtgatactg tagcaagttt acacggacaa gatcttcaaa tgacttataa
     1561 catgtatgta aatgacagcg cagctgttgc caatccatta acaaatagcg tggatttcgt
     1621 ttataacaac aatccattta atcaggaaga acaccacgaa aaaacgaaag ctgacgttgt
     1681 gacttatggt gcaaaattct taaaagtcga tagcggacta tttggaacag gaatcaaagc
     1741 aacaccgctt gaaggcgcag aatttgctgt taaaaacgca gaaggaaaat attatggtgg
     1801 attagttgat acggataaag acggtgtcaa agaagcagta tgggtagaca atgtagcaaa
     1861 tgcagcgatt ctaaaatcag ataaagaagg gcactttgaa atcactggtt taactgaagg
     1921 tgaatattca ttggaagaaa caaaagcgcc tgaaaactat caaaaattaa cagaagatat
     1981 tgcatttaaa gtaaacaaaa actcatttaa agaggaaaat cgaatcactg ttaaaaataa
     2041 tcaaaaagca gctattccat taacaggtag taatggtttc caaacgtatg tattgatctc
     2101 ttgcttgttg ctaggagcag ggggactttc tgcagttgtt tactttaaga agaaagccta
     2161 gaaggatcag ctaatgatta ttcgtaagcc cccaaaaaga agagtcatgc acaaactgat
     2221 gatatttttt tggttgctgt cggcagtagc agtctattta attccagttg tggaaacaaa
     2281 tgtagcgaaa tttcaacaac agcaaagaat tttagccatt gaaaatcttg aaaaaacaga
     2341 ttcttctttg gatgttgcga aaatagagtt aggcgatcct gttggtgttc ttacaattcc
     2401 aagtatttct ttaaaactac caatttatga cggaacttcc gacaaaattt tagagaatgg
     2461 tgtagggatt acggaaggaa ctggcgatat tacaggagga aacggtaaaa atcctttaat
     2521 cgcggggcac agtggacttt ataaagataa tcttttcgat gatttgcctt cagtaaaaaa
     2581 agggcaaaaa ttctttatta aggtaaatga agaacaacat gcctatcaaa tttatcgaat
     2641 tgaagaagtt aaagcagaag aattacaaaa aaattattta gattatctta tgcctgaaaa
     2701 agaccaagat aggatcacgt taatgacatg cacaccaaaa ggtgtcaact cacaccgctt
     2761 tttagtgtat ggtgaacgag tttccttcag taaagaagat ttaaaactac agaaaaaagc
     2821 aaaaaatagg aaatcaattt cattggattg gcgattagca ctgctattcg ctttttttgc
     2881 gttcctatta tttttattta taaaaaaata aggaggagat cggaatgaat aagagaaaat
     2941 ggctgtttgt ttgtgtagca gctctctttt ctactgtagc ttattcgcaa ctgtctttcg
     3001 ctgacagcag tgatagtagt accactcaaa caatagataa ttcaattact gcaccaagcg
     3061 aaacggacag ttcggagaat actacagaag aaacggcaga ttcaaatata agctttactg
     3121 aatcaacgaa tccaacgtca gaaagttctt cagagacttc agaaaagccg caagacgtag
     3181 aagatacgca agtgatcaag caagccattc atattcaaaa aattatttct tcgaatcaaa
     3241 tcaacaatca aggcagtcaa gtagcccagc aagaaggtat caatggcgca aagtttattg
     3301 tctacgatat cacgaatatc ttagaagaaa tgtcacaaga tgataatgag aaaaatcaac
     3361 ctgtagaaat aattgagtca aaattgaaag atcgagcaaa aaaactttca tacgaccagt
     3421 tgaaaaaagt ggctgaagga gaaaccaaaa cgattgatga tcaggaaggc gtgattgaat
     3481 tttccattga agtacctgca aacaaaaaac aagcatacta tatcgttaat gaatcaagcc
     3541 ctgaaaacat ttcaaacagt gaagatattg ttttattgac acctgtttca aatgaaaaag
     3601 gtgaattttt gacggatgtt tggttatacc ctaaatcaga gaaatcagag ccgaaagaag
     3661 aaattaaaaa agttgtgtca actggtgtaa agaagaactt ttttgaaaat tgttgggaat
     3721 tttttgctca attatttttt gataattagg atgaaatgat gaaaataata agaatattag
     3781 tttttaatct atccttttta gccgcaacat tattgtgttg cggtttaatt ttttctcaat
     3841 tatcaattta tccaagatat caacgattgt tcattcattt tctaaacatt caatgtgctt
     3901 ccatgccatt agaggtgatt aaaaagctct tgatcagtcc aatttttatg gttagtttgg
     3961 ttcttcttac aggattggta acgtgggtat taggaaaaaa agaatgaaaa gcttacgatc
     4021 gtttaaaaaa atattagatg gagggcatta tggaaaataa aattcattgc actacgggaa
     4081 gagtaattac tttagtgttg tttttgttcc ttcttctttt tgcgagggga actgcagtcc
     4141 aggccttaga aaaagttgat ccgagtcaaa tcaaagaaaa taataatctg gtgctttatc
     4201 atgatagtga ttcattgaga aatatcgaag taactgggat caatggattt aagatactag
     4261 atgagtattc aactaaaaaa caaaaagtac ttgcttggcc aaaagtcaaa gatttgaaaa
     4321 gtacgtcaaa aatcgttgcc cattatcaaa aagtaggtac ttatctagga aaagaaattg
     4381 attgtgacgt gactttttct aattttgtag cgcacgctgg ggttaaccaa gacggattag
     4441 ctaaaacaat taatggcaaa aagtattata attatttgct ctttgataaa aatatttatc
     4501 gaggattttt catgcagtca atcaatgtca tgaatgcaaa actagaattt aagtatgcag
     4561 gaacaaaaac aaaagtagct ttgagtgaac cacagccaga aattcaatca aaaatgagat
     4621 cgatgagagc agctggtatt gatttaaacg atacatttct atccatttat agtttaaatg
     4681 gacagtatca gcaacgtgtg tcttggtata atgacaataa tgaatctgtc ggtgaacgta
     4741 atattgatat gagagaattt gttgggtatg aaaaaatggg tagcttacct tattttgtca
     4801 caacagatac agcatgtgca gaataccaag ctcctgcgtt atcaacaaac aatttaactt
     4861 caaaagtagt gggaggacgc gcagaaaagg cttatagctc gaatgatcat ttcaccgatg
     4921 ttgtaggagc tgatacttat cacagaagtg gtgtaacgta tacgcttcaa ggcgcttccc
     4981 caacattcat gattggcgca aatacgaata gtatgatgtt tagctttgat actgcattgc
     5041 tatggacacc acaaccatcg aagcctacaa aagaagtgtt taacaaagct aatactgaag
     5101 aggcagcaca caatattgac aaaaaagtga ttccacaagg atcagatgtt tactatcata
     5161 ttcaccaaaa gtttgatgca ttaacagtca acacaatgaa caaatacaaa tcatttaaaa
     5221 tcactgatac ctttgacaac aaaaattttg atatggtatc ggatggggaa aaatatgatg
     5281 gcgcattgtg gtatgttgaa gcgaatggta cttggaaaaa agctgataca ggaggaaaat
     5341 taactgtatc aggtaataca gtcacttata ctgcttctga cagcttttta gctaacgctg
     5401 caaattacgg tagggattat attttgcagc tccatatgag agcaaaaaat gatgcaaaag
     5461 gaattgcacc aaatgtggct actgtaacgt ttaacaatga tccgaacaaa ggaacacaaa
     5521 aaacaaatac agtagaaaac tatttctctc ttaaaccaaa taaaaaagtg gaacaagatg
     5581 ggaaagatgt tgatggtcga aatgatggta aaaaaggaac gcctacagct cctttaaatg
     5641 caggatctga agtacaatat ctagtcacac aaaaatggca tacaaaaggt gtagacattg
     5701 cgctcgatca ttacaagcaa tttagtattc aagatccagt ggaagctcgt ttgacgtata
     5761 aagaaggttc tgcgcgagtg atagatatga gtacaggaaa agatattact tcagaaggta
     5821 cactaaccta tgatagccat tcaagaacgc tgaaatggga agcttccgct gatttcttaa
     5881 ataacaatct tttagatgga cgagaaattc aactgatatt tacagctaaa actcctttga
     5941 atgaagaatt ggatatcgac aaccaagcaa aagtttcagt agaaagtatt gagaaagcaa
     6001 ctaatgtagt aacgattggg gtaaaaccta acttacctca gatcattgtg ccaaaaactg
     6061 gatcgaatca tttagtgcta actgttattg gaagtatatt atttcttact ttagcatcat
     6121 tgggctatgt agttttaaaa ctcaaataaa tataaaaaca aggctgattt tcagccttgt
     6181 tttcttttat ttgaagattg gattcgcact aataaataca ttatctacgc caattctacc
     6241 agcagagtca actacaaaat tatgacctaa tccgagaata ctttggttat tataaagtaa
     6301 atttgcatta tgggcagggc tatttttcca tcctaccatg gcatcgatag caccatcgtc
     6361 tgataaaagg tcatcaatat acagcccagt ttgacttatg ttttctccct tagcaccata
     6421 gcagcctaag ctttccaata aatcttggaa agtaccacca tcagggcgat agtgactgaa
     6481 gctgacttgt aattctttag ctcgaatatc tgcagcctgt tgaacaatat cattgaattg
     6541 cattggggca acaccatttt gttggcgata atcatttaac caagcaagtt ctttttgacg
     6601 cataaagtta cgtaattcgt ctaatgaaat ctttttatat ttgaatgtga ttgttttatt
     6661 ttgatcgacg ttattaatgg atcgttggcc gtttttatcc acggtaacgg taggatcgtc
     6721 atttctgcac aagaaataat tttgatcagt catgtttttc caaggagtag caaagctatc
     6781 gccttcgtat actgtttttg attcactaga gagaacagcg ttggtatcag catttacatg
     6841 attaacagtc acattaaatt tacgagtgta gttgaaaata taattttgac tttcagtcac
     6901 attgtttaag gttactgaat ttgttggttc tacccgatcg tctaaaacat atccttgaat
     6961 agaaggagca gttaaagtga cactgtcacc gtgagtgaca ttgactatct gttctttcaa
     7021 aatattaact cgtccttcgt ttttgtagtt gttattgcac aacaaattga tttgatgcac
     7081 ttgattataa ttgaatgtga ttgttgtatt acctgtgatt gttttgcttt gagtatcatt
     7141 accaatcact ttgtagctaa aattatcttt agcgccttcg ctatatttaa atgcttttgc
     7201 cttagcagtg taagtagaac cagaagtcac ttttgcttga tctgtagcaa tcacttgacc
     7261 tgtttgattg tcaatatggt taatggtaac catatattct tttggcgttt caggtttgtg
     7321 ttgcaaccct ttaaatgcag catctagact atttttagca ttatttactt gggcttgaga
     7381 agccttagaa tcgtctagta cttttttagc attggataaa gcagtctgaa aattattcca
     7441 agattcttct gtaaaatctc ctttagcagt tgattttact cggttgtata aagcttgtaa
     7501 ttcagcttta ttcacagtag gaactggttt tttctttaat cctttgtaag cagaatctaa
     7561 tttatttttg gcactatcca cttgtttttg agtcgctttt gagtcattta acactttctt
     7621 agcattgttt aaagcgtttt ggaatgcgtt ccaggtattg tcggtatagt cacctttttt
     7681 agttccctta acgctcgtat aaagagcttc taaagctgat ttattcactt tgggtttaga
     7741 ggtgtcagcg ccataccaac cgataccttc gtatttccag ccatagtctt tggttaacca
     7801 ttgtacttct ttccaacttg cagtgtagtg atgtgagcca gattttacac caggaaggaa
     7861 aaggcggtag atcggtttat tatttttttc ggcagaacgc caggcaggcc cttcataagt
     7921 ccagccgtaa tttttagtca accatttcac ttcatcccaa ctagctgtgt aatggtgatc
     7981 acgaagacct ttattgtaca aacgatagac aagatggcca ccgctagtcg gtgcttccca
     8041 ggcggggcct tcataatttc cccattttat ctttactaga tggtcacgtt cttttttgct
     8101 agaggtgtaa aaatgttcat tattatttgg attgtaaagg cgatacatga cgttagttga
     8161 agcagcgtca acagatccag caagagcaag tgtggcagcc agtgtgacag caaatgttcc
     8221 agctataaca gttttttggt ttaataattt catgttttca actcctaaat agattaaatg
     8281 tttttacaag ttaattatat gtcggtatac cggcaaagtc aagagtaaat gacggtatac
     8341 cgattaaaat gcaataagaa accaatactt ttgaataagc attggtttct tattatttgt
     8401 tttcatataa gttttttcgc attttcgcca ttttagtgag tttgactgca ttatcaaaac
     8461 gcattttatc gattgaggag gttttattaa tcaagtcgat aactgtagtg cgcgcgacac
     8521 ctgattcctt atatatatct ttaacggaga ttccactttc aaataataac cattcgattt
     8581 catttttatc tgcgtacatt gcaatgctcc ctcttgattt tttctttatt atagctcttt
     8641 tttgtaataa atcacaatat caagagaaaa agtttctata gaaaggtggg aatttatgag
     8701 tatccgttcc ccgtgtatta aaatgaggtt tgttgctact ttcgttattt gatatactaa
     8761 aataaaacaa cgaaagaagg aataaaatga agaaaaaaca actatggttt gtctctatgt
     8821 ttgcaatatt ttttattatt ttaagtggat gttcaagtaa taagaaatcg gaagctaaag
     8881 aagataatag tttattgcaa ccaggaactg tttggaaaga agaagtaggc ggtcttgtta
     8941 ataatttgaa aattatagac gatactacat gggaatattc tgaaagtgta tggcatccag
     9001 atcctgtaca aatatcagtt aagagacaaa aagactataa aggtttagaa agatacaaaa
     9061 tagtagattc tgcaggtatt agcgacttta tcaataaacc agattcttta tttatcgttg
     9121 ttccttacga gaaagatggc gtaaaaaaaa tggtttttct tggatctagt aaggatgaaa
     9181 aacgaacgaa agagaaatta attcatgatg gatcacaatc aaataaatac aa
//
LOCUS       DACDYI010000097         8957 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.097, whole genome shotgun sequence.
ACCESSION   DACDYI010000097 DACDYI010000000
VERSION     DACDYI010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.097"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            453..1025
                     /locus_tag="IWM52_002608"
     CDS             453..1025
                     /locus_tag="IWM52_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP9974794.1"
                     /translation="MTENKATQERTPQERLQEEQEHKEHVHHTKINAAAIADHLLGNM
                     HTLHVKLHQYHWYVKGANFFTLHEKFEELYNENEKWFDKLAERLITSGHKPASTTVEF
                     EKYSMLSEDAVNKYAKAEDMVENIIEDFRSTRDLTIRAIRLAQDEGDDALEDTLIAFK
                     NDLDKNIWMLQAFIGKEALEDDDALDEDED"
     gene            1257..1874
                     /locus_tag="IWM52_002609"
     CDS             1257..1874
                     /locus_tag="IWM52_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006845390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP9974795.1"
                     /translation="MNYIAEKAHTKQYQKGEFLFRSQEKEDTLYIINRGKIRIYRLAD
                     SGKEQLVRILNPGDFTGEWTLFNPDAVHEEYAEATRDTVVCMIQQKDVQDFLEQYPAI
                     SLKLLAEMSKRLESSERQTTQVAVESVITRLVLFLAENVEPEMGNSPTITLPMAKKDI
                     ASYLGTTPETISRKFGELEDEGLIQQLSGKRIKIQDLDDLLLYSE"
     gene            2102..2440
                     /locus_tag="IWM52_002610"
     CDS             2102..2440
                     /locus_tag="IWM52_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003102891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974796.1"
                     /translation="MHCFWVIVAITLSAAGAFFVVPFTKNTIGGEAKTSNVVEADRLA
                     MEKEEEVVKGAGVIGHGKVAYYEASDQGDKSVFTVWFPWSLAGILFSITMTRMTRKIE
                     EMEQIEWDSY"
     gene            2425..3279
                     /locus_tag="IWM52_002611"
     CDS             2425..3279
                     /locus_tag="IWM52_002611"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003125438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP9974797.1"
                     /translation="MGFVLGVLNPHVVEIGSVVIHWYGVIIAAGILAALQLSTKEAKK
                     KGLEEDTIIDMALWAIPIGLLGARLYYVLFELGYYLQNPGQILAIWNGGIAIYGGLIA
                     GGGTLYWYAKKKGTSLALVLDILAPNVLLAQSIGRWGNFMNQEAHGGPVTRQFLENLY
                     LPEFIIEQMNMNGTYYHPTFLYESLWSLLGFVLIVTIRNRKQLLRQGEVALSYVLWYS
                     VGRFFIEGMRTDSLWIGEWIRVSQALSLALFIGAIVVWFIRRQDYPPISYYSDGNKGQ
                     KKTIKMVN"
     gene            3426..3659
                     /locus_tag="IWM52_002612"
     CDS             3426..3659
                     /locus_tag="IWM52_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HAP9974798.1"
                     /translation="MEKAILQLETLSCPSCMQKIEGAVKSVNGIDKDSIKVLFNSSKV
                     KTNFDSAVTSIEEIQKAIENVGYPVLKAKVKAV"
     gene            3857..5761
                     /locus_tag="IWM52_002613"
     CDS             3857..5761
                     /locus_tag="IWM52_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP9974799.1"
                     /translation="MQQYILSKKNVITVISGLLIVLGFFSHFVLENVGLSEWSLIIAS
                     VFGITPIAIQAFQAMKVKVISIDVLVSIAAIGALFIQNYEESAIVTFLFLFGHYLEQR
                     TLNQTRSAIKELTEMAPESALKQMDNGEFEEVEVDDIDEGDILLVKTGAKVPVDGIVL
                     TGEGHINEASITGEAIPVNKLADAEVFAGTILENGTIQIRADRVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKYYTPAVLVLAIVVWAFSQNIELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARNGVLLKGSEVIQDFSNVDTIVFDKTGTLTVGNPTVAATELYEKDPA
                     ETLGYLASVERESDHPLAKAVLNQIGETNFYPVEGTEVVKGGGIVSSVAGHRVAVGNV
                     ALMEKENVTLSKKVQKDVKRFEQQGNSLVLTAVDGELKVLMGIRDQVRPGVKANLQEL
                     KDLGVKNLVVLSGDNQGTVDVVAGELGLTEAHGYMLPEDKSAYIGKLQQRGQIVAFVG
                     DGVNDSPSLALADIGIAMGSGTDVAIETSDVVLMNSNFSNLPHALGLVKATANNMKQN
                     IVISVGVVLVLLTSVFFSEWMNMSIGMLVHEGSILVVIFNGMRLMKYKLKGRKEQKEV
                     SAPTEKVKIS"
     gene            6115..6480
                     /locus_tag="IWM52_002614"
     CDS             6115..6480
                     /locus_tag="IWM52_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9974800.1"
                     /translation="MTAEVCTTTCIHKEKVASVKNNLKDKDLSTLLVLGKCFSDSSRI
                     KIFYALETYKEMCVCDLAAILNASIATTSHHLRFLKKNGMAKSRQYGKVVYYSLANEE
                     ILSAVQAFLKLSENSSMKA"
     gene            6497..7108
                     /locus_tag="IWM52_002615"
     CDS             6497..7108
                     /locus_tag="IWM52_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HAP9974801.1"
                     /translation="MLQNILSALAVYISTSIDYLFILLIIFSQNHTKKGLRQIFFGQY
                     LGTGILVAISLFAAYVLNFIPQDWIIGLLGLIPIYLGIRVAFVGEEEEEEGEVVEKLG
                     SRGTNRLFWTVSLITIASGGDNLGIYIPFFTSLSFSEIVTSLIVFAISVAVLCYISYK
                     FAKISFVSETLEKYERIIVPIVFIGLGIFIMIENGTIQTILGL"
     gene            7136..8797
                     /locus_tag="IWM52_002616"
     CDS             7136..8797
                     /locus_tag="IWM52_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAP9974802.1"
                     /translation="MSENKKYHEWILEGIDCANCADKVERGVAKVAGVANSSVNYMTE
                     TLSFEVTEEKEYLVLSNVQQKVKKLEPDITLKNKVDGRLIEINPALIKKSNLKDDKKN
                     YEWILEGIDCANCAAKVERGVATVPGVTNSSVNFMTETLSFEVAGENKTDVLASVKQT
                     VGTLEPDVVLRKKSDGTLIEHGTDSNKNSTSNKSISDKDETNQKQTNRAYLASLLGNK
                     TKITLIRLILGLGILATGIFLPMDGQASLGLFILAYLITGYDVVGRAIRNIFRGQVFD
                     ENFLMTIATLSAFYIQEYPEAVAVMLFYQVGEVFQDIAISKSRRSIADLMDIRPDYAN
                     LVTGNNVEKVAPEAIKIGNIILIRPGEKVPLDGKVVEGASAMDTSALTGESVPRSVKP
                     EDTILSGFINKNGLLKVVVEKPFSESTVKKILDLVQNASGRKAPTEQFITKFSRYYTP
                     IVVIAAALLAIVPPLLVPGATFNEWIYRASIFLVISCPCALVVSIPVGFFGGIGSASR
                     KGILVKESNFLEGLNDLKYVVMDKTGTLTKGFCCKVLNLLSNKVE"
     gene            8825..>8957
                     /locus_tag="IWM52_002617"
     CDS             8825..>8957
                     /locus_tag="IWM52_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9974803.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     "
BASE COUNT         2891 a         1448 c         1957 g         2661 t
ORIGIN      
        1 gtatacccta aatagacatc ataataacat tgaaaaacat ggttgtcatt tttctagata
       61 aatggcataa attttcagcg ctaagtgaca aaagcaacta atttttcatg agagattttt
      121 aaatatctca atctatctcc aaaattagtt gtttttgtag ctatagttga ctatgaatat
      181 gacaaataat tagaaaaaac tgtgccaatt aagtataaaa atgacatttt aggccctact
      241 ttaatgaaat ttttgtttgg agggaacgac ttcattcctt tgattatttc agttgaattt
      301 aatagagaag cctggcgtct attaagaaat aattttcttg atgagcgtca atttttttaa
      361 cggaaatcta tcctatacta aattcagtaa agaaaaagga ggaatcaaag aaagctattt
      421 taaacaaacc aaaattatag gaggaataaa tcatgactga aaataaagct actcaagaaa
      481 gaacacccca agaaagattg caagaagaac aagaacacaa ggaacatgtt catcatacga
      541 aaattaatgc agcggccatt gcggatcatc ttttaggcaa catgcataca ttacatgtga
      601 aactgcacca atatcactgg tatgtaaaag gagccaattt ctttacgttg catgaaaaat
      661 ttgaagagtt gtataacgaa aatgaaaaat ggttcgacaa acttgcggag cgtctaatta
      721 cttctgggca taaacctgct tcgacaacgg ttgaatttga aaaatattcg atgctttcgg
      781 aagatgcagt taataaatac gctaaagcag aagacatggt tgaaaatatt atcgaggact
      841 tcagaagcac tcgtgaccta actattcgcg cgattcgttt agctcaagac gagggtgacg
      901 atgctttaga agatacattg attgctttta aaaatgactt agacaagaac atttggatgc
      961 tgcaagcgtt cattggtaaa gaagcattag aagatgatga cgctcttgat gaggatgaag
     1021 attaaggtaa aagtagaagc tgatttaggg attggaattc tttccagtcc tttttccatt
     1081 ttcttgatgt gcatcaattt ttttgtagta agagacttat ataataaaag cagcgaatat
     1141 aaacaaaata aataaagggg gagaatgaat tgactaaaca tacccatcat gaaattggcg
     1201 accacgcagc ctgtatccgc ttggttccaa tattcaatca cttagaggat agcgcgatga
     1261 attacattgc tgaaaaagcc cacacgaaac agtatcaaaa aggagagttt ctgtttcgtt
     1321 cacaagaaaa agaggatact ttatatatta tcaatcgtgg gaaaatccgt atttatcgat
     1381 tggcagactc tggcaaagag cagctagtgc ggatcttgaa ccctggtgat ttcaccggag
     1441 aatggacctt gttcaacccg gatgcagtac acgaggaata tgccgaagca acccgagata
     1501 ccgttgtctg tatgattcag caaaaagatg tccaagattt tctggaacaa tacccagcta
     1561 tttctttaaa actattggcg gaaatgtcta aacggttaga aagttcagaa cgtcaaacga
     1621 cacaagtagc cgtggagagt gtcattaccc gtttagtttt gtttttggca gaaaatgtgg
     1681 aacctgaaat gggcaactct cccaccatca ccctgccgat ggcaaaaaag gacattgctt
     1741 cctatttagg aacgacacct gagaccatca gtcgcaaatt tggagaattg gaggacgaag
     1801 gattgattca acagctgtct gggaaaagga tcaagattca ggatttagat gatttattgc
     1861 tttacagcga ataatcggac acactttttt ctacacgttg gtgttctgtc gctcttcatt
     1921 aacaacggga cagaactcag tgcttttgct tccatctgtc cgtttttggc ttttttaaga
     1981 cagaacgacc ttcattcatt ggaaaaataa aagcacaaac atgataaccc tcaagttttt
     2041 ttgttttatt tttctttaag atagggttgt cgaacaaaac gtaaaaataa gtagggtgaa
     2101 tgtgcattgt ttttgggtga tagttgccat cacgttgtca gcagcggggg cattctttgt
     2161 cgtccctttc accaagaaca cgattggcgg cgaagcaaaa acgtctaacg ttgttgaggc
     2221 agataggtta gctatggaaa aagaagagga agttgtaaaa ggagccggcg taatcggaca
     2281 cggaaaagtg gcgtattacg aagcgagtga ccagggagat aaaagtgtat tcactgtttg
     2341 gtttccatgg agcttagccg gaatcttgtt cagcatcacc atgactagaa tgacgcgaaa
     2401 aattgaagaa atggagcaaa tagaatggga ttcgtattag gcgttttgaa tccccatgtg
     2461 gtggagattg gttctgttgt gattcattgg tatggagtca ttattgcagc gggcatatta
     2521 gcggctctcc agttgagcac caaggaagca aagaaaaaag gactggaaga ggataccatt
     2581 atcgatatgg ccttatgggc aattccgatt ggactcctcg gcgctcggct ttattatgtg
     2641 ttatttgaac ttggttatta tctgcaaaat ccaggacaaa tccttgcgat ttggaatggc
     2701 ggcattgcga tttatggggg attgattgca ggagggggta cgctatattg gtatgcaaag
     2761 aaaaaaggta cttctttggc actcgttctt gatattttag cgcccaatgt attgttagcc
     2821 cagtccattg gtcgttgggg caattttatg aatcaggaag ctcatggtgg gccggttact
     2881 cgtcaattct tggaaaatct ttatttacct gaatttatca ttgaacaaat gaatatgaat
     2941 ggtacctact accatccgac ttttttatac gagtcattgt ggagtttgtt gggctttgtc
     3001 ctcatcgtca ctatacggaa ccgaaaacag cttttgcgtc aaggagaagt cgctttgagt
     3061 tacgttttgt ggtactcagt gggtcggttc ttcattgaag gtatgcgaac ggacagttta
     3121 tggattggcg agtggatccg cgtttcgcaa gccttgtctc tggccctgtt tattggcgcg
     3181 attgtagtat ggtttatacg cagacaagat tatccaccaa tttcttatta ttctgatggc
     3241 aataaggggc agaaaaagac tattaagatg gtgaattgag caaaaagata aaaagaagtg
     3301 caaaacttga tatgcgtcaa ttttcgtttg ctaaaagtga gctatactac agttgtaaag
     3361 aggaaaaggc agtgttcatt aatctgctgc cgataaaata aaaacacata aaggagatag
     3421 gaattatgga aaaagctata ttgcaattag aaacgttgtc ttgtccaagt tgtatgcaaa
     3481 aaattgaagg tgccgtgaaa tcagttaacg gtattgataa agacagcatt aaagtattat
     3541 tcaactccag caaagtcaaa accaattttg attcagctgt cacttcgatt gaagagattc
     3601 aaaaagctat cgaaaacgta ggctatccgg ttcttaaagc aaaagtcaag gccgtttaac
     3661 agattaaatg aactaggcaa ttctggaaga aaagctattt ttccaggatt gctttttttg
     3721 ttgcaatccg attcgaggtg cttagaaacg ataaaagaga actttctaaa cttgatgtca
     3781 gtcaattaat tacttaggca attatcttat actgtgttta tcaataaaag agaaattaaa
     3841 tttggaggga aaaagcatgc aacaatatat attaagcaag aaaaatgtta tcaccgtcat
     3901 cagtggattg ttaatcgtac tcggtttctt cagtcacttt gttttagaaa acgtaggact
     3961 ttcagagtgg tctttgatca tcgcctctgt ctttgggatt acgccaattg ccattcaagc
     4021 gtttcaagca atgaaagtca aagtcatcag tattgatgtc ttagtcagta ttgcagcgat
     4081 tggtgcgctt tttattcaaa attatgaaga atcggctatt gtcacgttct tattcttatt
     4141 cggacactac ttggaacaac gaacattgaa ccaaacgcga tctgctatca aagaattgac
     4201 tgaaatggca cccgaaagcg ccttgaaaca aatggataat ggcgaatttg aagaagtaga
     4261 agtggatgat atagatgaag gggatatctt gctcgttaaa acgggtgcga aagttccagt
     4321 agatggcata gtccttacgg gtgaagggca tatcaatgaa gctagcatta caggtgaagc
     4381 tattccggtc aacaagctag ctgatgcaga agtttttgca ggaaccattt tagaaaacgg
     4441 aacgattcaa atcagagctg accgagttgg tgaggacacc acatttggaa aaattattga
     4501 actcgtggaa gaagcacaag attctaaatc cgaagcggaa cggttcattg atcgcttttc
     4561 taaatactac acaccagctg tcttggttct ggcaattgtt gtatgggcgt tcagtcaaaa
     4621 tattgagtta gcaatcacca tcttggttct aggttgccca ggcgcattag ttatcggagt
     4681 gcctgtctca aacgttgccg gtattgggaa tggtgctcgt aacggtgttc ttttaaaagg
     4741 gagtgaagtc attcaagact tcagtaatgt ggatacaatt gtatttgata agacaggtac
     4801 tttaactgtc ggaaacccaa ccgttgcagc gactgaactg tatgaaaaag atcctgctga
     4861 aacgcttggc tatctggcca gtgtggaacg agaatcagat catccattag caaaagcggt
     4921 cttaaatcaa attggagaaa caaactttta tcctgttgaa ggaactgaag ttgtgaaagg
     4981 cggcggaatc gtttcaagtg tagctggaca tagagtggct gttgggaatg tggccttgat
     5041 ggaaaaagaa aatgtcactc tcagcaaaaa agtgcaaaaa gatgtcaaac ggtttgaaca
     5101 acaagggaac tctctcgttt tgacagcggt cgacggtgaa ttaaaagtac tgatgggcat
     5161 tcgcgatcaa gtccgtccgg gtgtgaaagc taatttgcag gaattaaaag acttgggcgt
     5221 gaaaaatcta gtcgttcttt caggagataa ccaaggaacc gttgatgtgg tcgccggcga
     5281 acttggtttg accgaagctc acggctacat gttgccggaa gacaaatcgg cttatatcgg
     5341 aaaacttcaa caacgtggtc aaattgtagc ctttgttgga gatggcgtta acgatagtcc
     5401 gtccttagct ttagcagaca ttggaatcgc aatgggaagc ggaacggacg tagcgattga
     5461 aacatccgat gtcgttttaa tgaactcgaa cttcagcaac ttgcctcacg cattaggatt
     5521 agtaaaagcc accgcaaata atatgaaaca aaatatcgtg atttcagttg gagtagtgtt
     5581 ggtcttgttg accagcgtct tcttcagcga atggatgaat atgtctatcg gaatgttggt
     5641 tcatgaaggt agtatcttgg tggtaatttt caatggcatg agattaatga agtataagtt
     5701 gaaaggtcgg aaagaacaaa aagaagtatc agcacctaca gagaaagtaa aaatatctta
     5761 agaataaatt agaagaggct gagacaatag tccatccttg aaatgacaaa agcggaaact
     5821 cagattttta aatctgggat tttccgcttt tttgagttct tgaaaaaaaa taagagtata
     5881 gagtatgttc tttataaaat taattttcgt gtaaattatt aaaaatttaa taataaaatt
     5941 taaattccta ttaagttgta acacatgaaa ttgcttaaaa tagtgtatca aaaaccttgt
     6001 tttattaatg ttgaatttat ttgaaaggtc tacagattaa catattattg acaaaacaat
     6061 atttaaatag ttatacttca ttcaaacgtc tgtttgaacg aggtggtgaa gttaatgact
     6121 gcagaagtat gtacaactac ttgtatacat aaagaaaaag tagctagtgt taaaaataat
     6181 ttaaaagata aagacctttc aactttactt gtattaggaa aatgttttag cgattcttct
     6241 aggattaaaa ttttctatgc tttagaaacg tataaggaga tgtgcgtttg tgatttagca
     6301 gcgatactta atgctagtat tgctacaacg tcacaccatt tacgtttttt gaaaaagaac
     6361 ggaatggcga aatcacgtca atatggaaag gtagtttatt actctttagc gaatgaagag
     6421 attctttccg ctgtgcaggc ttttctaaag ttgtcagaaa atagttctat gaaagcttag
     6481 tttggaggta gttattttgt tacaaaacat tttatcagca ctcgctgtat atatttctac
     6541 cagtattgat tatttattta tcttgctgat tattttttct caaaaccata ctaaaaaagg
     6601 tctccgtcag attttttttg gtcaatatct tggaacagga atcttggtag ccataagttt
     6661 gtttgcagca tatgtgctaa attttattcc tcaagattgg atcattggtc ttctgggact
     6721 aattccgatt tatttaggga ttcgtgttgc ttttgtgggc gaagaggagg aagaagaagg
     6781 ggaagtcgtt gagaagctag gatctagagg aacgaatcgt ttgttttgga cagtttcctt
     6841 aataacgatt gcttctggtg gcgataattt agggatttat attcctttct ttacctcgtt
     6901 atctttttca gaaattgtca cttctttaat cgtctttgct atctctgttg cggttctttg
     6961 ctatattagt tataaatttg cgaaaatttc ttttgtctct gaaacactag aaaaatatga
     7021 acgaatcatt gtacctatcg tatttattgg attagggatt tttattatga tagaaaatgg
     7081 aaccatccaa actatattag gtttgtagag atcttattag taaaggaagc aaacgatgag
     7141 tgaaaataaa aagtaccatg aatggatatt agaaggaatc gactgtgcaa attgtgcaga
     7201 caaagtagaa agaggcgtcg ccaaagttgc aggtgttgcc aatagtagtg tgaactatat
     7261 gacagaaacc ttgagtttcg aagtgacaga ggaaaaagaa tatcttgttt tatccaatgt
     7321 gcaacaaaaa gtaaaaaaat tggagcctga tatcacttta aaaaataaag tggacggtag
     7381 gctgattgaa ataaatccgg cattaatcaa gaaaagtaat ttaaaagacg ataaaaaaaa
     7441 ctacgaatgg atattagaag gaatcgattg tgcaaactgt gctgccaaag tggaaagagg
     7501 agttgctaca gttcccggcg ttacaaacag cagcgtcaat tttatgacag agacactgag
     7561 cttcgaggta gcaggtgaaa ataaaactga cgtgttggct agtgtaaaac aaacagtagg
     7621 aactttagaa cctgacgttg ttctgcgaaa aaaatcggac ggtacattga ttgaacatgg
     7681 aactgactct aataaaaatt cgacttctaa caaatcgatt tctgataaag atgagacaaa
     7741 ccaaaagcag acgaatagag cttatctggc cagccttctc ggaaataaaa ctaaaattac
     7801 ccttattcgt ttaatactag gattaggaat attagcgaca ggaatatttc tccctatgga
     7861 tggacaagcc tctcttgggt tgttcattct ggcttattta atcaccggtt atgacgttgt
     7921 aggacgtgcc attagaaata ttttccgagg tcaagtattc gacgagaact ttctaatgac
     7981 cattgcgacg ttaagtgcct tctatattca agagtatccc gaagctgtag cagtcatgct
     8041 attttatcaa gtgggagaag tattccaaga tattgctatt tcaaaatctc ggcgctccat
     8101 tgcggaccta atggatattc gacctgatta tgccaattta gtcacaggaa acaacgtaga
     8161 aaaagttgct cctgaagcaa ttaagattgg aaatattatt ctcattcgtc cgggcgaaaa
     8221 agtgccattg gatggaaaag tagtggaagg tgcttctgcg atggacactt ctgctttaac
     8281 aggcgaatcc gttccaagaa gcgtgaagcc ggaagacact attttgagcg gatttattaa
     8341 taaaaacgga ttgctaaaag tagttgtaga aaagcccttt tcggagtcta ctgtgaagaa
     8401 aattttagat ttggtccaaa atgctagtgg ccggaaagca cctactgaac aattcattac
     8461 caaattttct cgctactata ctccaattgt ggttatagct gctgccttac tggcaattgt
     8521 tccgccattg ctagtaccag gcgctacttt taatgaatgg atatataggg ccagtatctt
     8581 tttagttatt tcatgccctt gtgctttagt cgtttcgatt ccagttggtt tctttggtgg
     8641 gattggttct gcttcccgca aggggatttt agtaaaagaa agcaatttcc tcgagggttt
     8701 gaacgatctt aaatacgtgg taatggacaa aacgggtaca ttgacaaaag ggttctgttg
     8761 caaagtttta aatctactat caaataaggt agaataatag aaaaagatag taggaggaat
     8821 gacgatgaat cattttaaag gaaagcaatt tcagcaggat gtgattattg tagccgtggg
     8881 atactatctt cgttataacc ttagctatcg tgaagttcaa gaaatcttat atgatcgtgg
     8941 cattaacgtt tctcata
//
LOCUS       DACDYI010000098         8592 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.098, whole genome shotgun sequence.
ACCESSION   DACDYI010000098 DACDYI010000000
VERSION     DACDYI010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.098"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(51..1517)
                     /locus_tag="IWM52_002618"
     CDS             complement(51..1517)
                     /locus_tag="IWM52_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAP9974804.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1904..2500
                     /locus_tag="IWM52_002619"
     CDS             1904..2500
                     /locus_tag="IWM52_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP9974805.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2558..3040)
                     /locus_tag="IWM52_002620"
     CDS             complement(2558..3040)
                     /locus_tag="IWM52_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974806.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3146..3343)
                     /locus_tag="IWM52_002621"
     CDS             complement(3146..3343)
                     /locus_tag="IWM52_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974807.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3638..4396
                     /locus_tag="IWM52_002622"
     CDS             3638..4396
                     /locus_tag="IWM52_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAP9974808.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4456..4884
                     /locus_tag="IWM52_002623"
     CDS             4456..4884
                     /locus_tag="IWM52_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974809.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4930..5002
                     /locus_tag="IWM52_002624"
     tRNA            4930..5002
                     /locus_tag="IWM52_002624"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4963..4965,aa:Gln,seq:ctg)
     gene            complement(5152..5523)
                     /locus_tag="IWM52_002625"
     CDS             complement(5152..5523)
                     /locus_tag="IWM52_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9974810.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5738..5923
                     /locus_tag="IWM52_002626"
     CDS             5738..5923
                     /locus_tag="IWM52_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974811.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6006..6194)
                     /locus_tag="IWM52_002627"
     CDS             complement(6006..6194)
                     /locus_tag="IWM52_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974812.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6218..6481)
                     /locus_tag="IWM52_002628"
     CDS             complement(6218..6481)
                     /locus_tag="IWM52_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974813.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6639..6896
                     /locus_tag="IWM52_002629"
     CDS             6639..6896
                     /locus_tag="IWM52_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAP9974814.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6967..7158)
                     /locus_tag="IWM52_002630"
     CDS             complement(6967..7158)
                     /locus_tag="IWM52_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974815.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7299..7619)
                     /locus_tag="IWM52_002631"
     CDS             complement(7299..7619)
                     /locus_tag="IWM52_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974816.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7642..8076)
                     /locus_tag="IWM52_002632"
     CDS             complement(7642..8076)
                     /locus_tag="IWM52_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9974817.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            8411..>8592
                     /locus_tag="IWM52_002633"
     CDS             8411..>8592
                     /locus_tag="IWM52_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9974818.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
BASE COUNT         2723 a         1695 c         1362 g         2812 t
ORIGIN      
        1 aaaaacagga actgctgcaa ataagcaatt cctgttttta tgttcattga ttaatgaata
       61 gagcccatta gttttttgat tggttctttc acagtaaata gcaagatacc accgacgatt
      121 gctactgcac cgataatacc gaagtaagca gattctgttc ccggtgtgta gaagcgtgca
      181 atttgtgcat tgattgcttg tgaagaagca tcagctaaga accaaatagc gattgtttgt
      241 gattcaaatg ctcgtggtgc taattttgtt gtcacagaca aaccaactgg tgaaagacac
      301 aattcagcaa taaccacgat gaagaaactt cccaacaacc acaatggact tacttttcca
      361 cttgtaccat aaagcatacc cggcaacatc aatagcaagt acgataatcc tgcaaataaa
      421 agtccaagag agaatttcac tactgtagat ggttgacgtt caccaagttt cgtccacatc
      481 gttacgaata ctggtgtcag aataaagatg aaaattggat tcaatgattg ataccagctt
      541 gcttggatcg ttacaccaaa tagcgtagat tgtgtacgtt cactagcaaa taaagctaag
      601 atagatgaac cttgttcttg taatgaccag aaaatgattg ctgcaaggaa taatgggata
      661 taagcccata ctcttggttt ttcttcagca gttacatctt ttgaagtcag cattttgata
      721 aagtaataca acggtaagat aatccccaat acactgattg tattgacaaa aaagtcaatt
      781 gtcagatgac cagttagttg tgccccgccg aatactgcag cgataacaac aagtgcgatg
      841 atcaagttac ggatgaatcc tttacgttct tcagttgtca aaggattcgg cggtgttgta
      901 cccactccgt ctaatgtctt gcgtccttgg atgatgtatt gtaataatcc aaagaacatc
      961 ccaatagcag ctaaagagaa ccctaaatga tagttatatt cttgaccgat tgtaccaacg
     1021 attagtggtg ctaataatgc ccctaagttg acacccatgt aaaaaatcga aaatcctgca
     1081 tctcttcgca agtcagtctt tgaataaaga tgtccgacca tgccagaaac atttggtttc
     1141 aaaaaggcgg ttccgataat aatcaatgcc atcgaaacaa acaatgtact tacaccaaat
     1201 ggtaaagcca agacgatgtg gccaagcata atgaaaactc caccgaagaa tacagtgcgt
     1261 tttgctccta acactcggtc agatacccat ccgccgacga tactagacat gtagatcaaa
     1321 gatccgtaga tcgacatgat agctaatgct gttgctcgtg gtaatccaag tcccccgttg
     1381 gcaactgtat cgtacatata atagataagt aatgcacgca taccgtaata actaaatcgt
     1441 tcccacatct cagtgaagaa caaagttgac aatcccttcg gctgcccgaa gaagcccttg
     1501 tcttgctgtt tttccataat ctatacccca attctttaaa ttttcagaaa atattataca
     1561 tctttaccga aaaatttaac taaaaaactt atttaagaaa tataaactaa ttttctataa
     1621 aagcaagtaa tttttaatgg caatatcata aaaaaaagaa gatagagatt taataattat
     1681 atatcagctg tttttttcaa ataagcaaaa attcctaaaa cacaacaatg aaagcgataa
     1741 ctaagaaaat ccttacataa cgatttttct tattaaaatt gttttttgaa cttcgcttat
     1801 tttttgtgag catatctgtt tacttacttt ttgtaaggtg ctatacttca tttcgtaatc
     1861 aattaaaaaa atgcaaaacg caaatcaaag gagaaatttt attatgacaa cttttactga
     1921 cactttgaaa aaccgccgtt caatctatca tctaggacgt aatgtttcac tttctaatga
     1981 agaattaaca actttgatca aagaagcaat taaagaaagc ccaactgctt tcaatgctca
     2041 atcaacacgc gcagttatcc ttttcggaga tgcacatgaa aaactttggg aaatcactga
     2101 agaagcactt cgcccattga ctccagcaga agctttccca aatacacaaa acaaattagc
     2161 tggtttcaaa aatggttatg gtacagtatt attcttcaaa gatactgacg tggtaaaagg
     2221 tctacaagaa caatttgaat tatatgcaga caacttccca gattggtcag aacaatcaaa
     2281 cggaatcgca acagctaata cttgggtagc attagttgat aaaggattgg gcgcaaactt
     2341 gcaacattac aacccagtta ttgacgaagc agttgcaaaa gaatggaata tcccttcaaa
     2401 ctggaaacta cgttcgcaat tagtattcgg ttcaccagaa acaccggctg gggaaaaaga
     2461 atacatgaac gatgccgatc gcttccgcgt atttggataa ttttatttga aataaaaaac
     2521 agaggttgtg acaaacatct tgtcgcaacc tctgttttta ttctggatca atcataatat
     2581 caatctctgc ctcatcgttt aagcgttggg acaaaaatgg aactaattct tctaatgatg
     2641 cagaaaattt cagcatcggt cctcgccatc cgtttcggaa aaaattcatt tttacgattt
     2701 tagaaccttt tggctgatat aatacaattt tattgatctc agaaaaagga acaccttttt
     2761 tgtcaaagct attcaaaaca agtccttctt ctgtgattcc tctactgtcc aacaaaaaac
     2821 taataaaaat cattgctgac aagatccctt ttgccaattc atctgctgta ggatgactga
     2881 tgagtgtata ccaaatgact agtgacacta agaagggtat caccaagtag acccatcgat
     2941 ttcgcacagt ccgataaata atttcttttc gaaccagcca aagaaataaa aacatcacaa
     3001 caacgaataa aaacaataga accgtaaaga atgttaccat gacaactcgc ctcgcttttt
     3061 cttctccaga aaataggaaa agaatactga tttaccttct caaagaaatt ggacttgcag
     3121 cacgccgcac gtccaattgt tttctttatt caaatgtatc atattttgca acaaccatgc
     3181 ttgctttttc atctaataat tcttggtcct ctgcagcaca ttgggcaact ttgaccacgg
     3241 cgcagtttgt catttttgat acaacttcac caatgaccgt ttttgtaaaa ccaattggtt
     3301 tgcatgcata ttgttctcct aaattaatca tttcttccgt catcaaaatc ctcctaaact
     3361 gctggaactt ttctccacat taccactgct atttctttgg agatttaact cattgatttt
     3421 tatttacagt taaaaatcaa ccttacaggc attatagcgt actgttttcg ttttcacaat
     3481 taaaacagct gttgtattta gaagaatttt tttcaaaaaa ttaaaacata ttccttaaaa
     3541 actaattatt tcctacatat taaaatataa tacttccatt tccaataaaa atacatttat
     3601 aataggaaag ataaacagct gaaaagggag atttcaaatg aagaaaaaaa cgttcaaaaa
     3661 actattgttt ctagggggca cagctgctct tttattaagc ggtacaagtt attacctaca
     3721 tatccattct ccagaacata caatgaacac tgcacaaaaa agttcaacac aaaaaacgaa
     3781 aaagacaaaa gagaaagcag aagttttact agatgttcca ttagagagcc agtttgattc
     3841 tccttcttta gaaaatggct gtgaagtcac ttcgctttct atgcttttat ctttttatgg
     3901 ttatgaaaca actaaaaacc agttagctga gcaattggat tacgtccctg ttttcaatgc
     3961 agatgggact catggcgatc ctaatgaagg atttgttggt gatatcagtg gcggagactg
     4021 ggctatggtc gtctatgttc ccccagttgc ctcccttgct cagcaaatcg tccaaacgga
     4081 ttatcacact tttccaaaaa ccgatgctca actcgatgat atcaaaaaag cactatctaa
     4141 aggaaatcct gtctggactt ctgtgactat caattttgaa gtcccagatg aaactgattt
     4201 tatgacttgg accacaaata atggagaagt aagagtcact cctcttgttc atgcttgtgt
     4261 agtcactgga tatgacaaag aaaatattta tgtcaacgat ccctatggtg taaaaaatcg
     4321 agcagtaccg atcaacgatt tttcggcgat tttcactgca atgggtggac agatgctaac
     4381 tttggagaaa agctagtaat tcaaacaaaa atcccttata ctcaagatac caaacctttg
     4441 aagggagcgt agataatgga aaaattcaca cataaaaaaa tggatccaaa tgaaattccg
     4501 attatttttg tccgtgactg caagggaaat gttcaaggta aagtctctat taatgagtgg
     4561 aacgagcgtc gcagacctgc taccctcaat gaactggaaa tcaaattgta tcgtcaatct
     4621 cttgtttatt acgcagatca agagtacgag aaagccaccg atttattgaa atttctaatt
     4681 gctcgaacag agtatactcg ctttgaatat attgaacgtt tagcaaacat ctatcatatt
     4741 atgaatgaac ctgtcaaaga atatcagtta ttggacaccg tcttgtcagt agcagaactg
     4801 attgcccttc ctgctggatt agaaaagaaa ctagtacgcc gccttttacg agtaaagcaa
     4861 caactatccg atcaagaaaa ataacttgcg tttttttagg aaagtgctat gattattttg
     4921 ttgaataaat gggatgtagc caaactggta aggcacttga ctctgaatca agcaattgta
     4981 ggttcgaacc ctgccatccc aatttgatgt gcttgctgaa aaaatgaacc ccctgtaaaa
     5041 gaacaatctt tttgcagggg gattattgta ttatttttac tacttgtatt tagtttacat
     5101 aattaaatta tgaataataa aatcgggcgt attcttaata caaaaactga tttacccaat
     5161 aattgctaat gctttctccg gaactttttc tctaggaact tcctcatacg tttctatgtg
     5221 tgctccttta ttgctaattt tcaaataatg attttctttt aacacagaaa gaccattaaa
     5281 ttcaaccgtt cgttctttcc cattggcatc agcagctttc tgcacatacc ttgctgtacc
     5341 atattcattt acttcctctg gtttttgtgt ctttacatag acctctcctt tttcaactaa
     5401 tggatttagc tgatcaagta taccagggat ttctccataa cttccttcag tataatattt
     5461 cagtccaaat agcaaaatac cactaatcgc cgccagtcca atcaaatatt taatcacttt
     5521 catctttcta cctccttgtt atatattcag tatatgggtc agttaaagaa attccatccg
     5581 tcccaagttg gagatttttc tcctacttta gaagcgagaa tcgagtgttt ttttagtaaa
     5641 aatgatcgtt atttgatatt attgacgtgt ggataaatca gtttttctcg ttttgtccat
     5701 gtaaaaaatc atgttgatag caaaggatgt gttcaaaatg aatcaagaag aattatttca
     5761 aaaagtcaag gaaatgatta agaacggcaa tttcgatggc gcaaaacgat ttatcgaaga
     5821 acataaagaa caattaggac cttataaaga gaaagcccaa aatcttttaa aagatgtaaa
     5881 tatcgacagt gtcaaaaaca aatttaaaaa tctattcaaa taagtaaaac tttcactttt
     5941 gtaaaatcaa aggaggacaa tgctgctgca tcgtcctcct ttggttcttt tctgaatact
     6001 tcatactaaa ataaacgtgt cggatcgtaa tgataagtca cattgccgtt cagtgaggct
     6061 tttcccacca catatttacg attctccaac ttcagccacg cttctcgaaa agccattaat
     6121 tcttctcttt ttacatcaat cttttcgatt tttccatcga tcaaatcttg aataagttgt
     6181 tcatatggat tcattttttt cctccatctg ctgtcaatca ataagttgta aaaataggtt
     6241 catatgcttt tttggtatct agatcttctc tgactttgac tgtcgtataa ataatttttt
     6301 ctactgggat actataataa cttccttcac cattattgaa tcggtgaacg tctttctttg
     6361 ctaaaatacc atttatttta ttcgtcagta cacgttgttc gacactgtta cttacaacat
     6421 agtaaatcac ccgaatatta ttgatcaaat gcaaatcgat ttctaacact acatgatcca
     6481 tgaaaatccc actccttctt tcactttgat tatagcaagt ttctcctcta atttcaaaat
     6541 cgatacagat tttaccggat aaatccacaa aactgcttgc gccatttcgc acaaagtgtt
     6601 attttgtatc atggacttta tttatcggag gcaatagtat gatcgcgatc ggtcaatttg
     6661 ttttttatat tccttttttt attatgataa gcattctttt ctattacata aaatggacaa
     6721 aaaagaagtt ttcagtttta ctggcctctt tacctgctgt atattttact tatcaaattt
     6781 tttcttttag gcattgggaa acaacgtctg tattaataac acacatcatc gaattgacgc
     6841 tttcagtaat ttttcttatt atttggatct attttttata taagaatcag aattgaatcc
     6901 ctcgcatgtt taaaaagaaa acgattaaag atgcagcaaa tttctgccga tctttaatcg
     6961 ttttttctat tctgttatac caaaaaaatg tttgaatcgg tgaaggatcg ttccttgttt
     7021 taattctata aattcttgtg cgatcgggta cttttcttcc aaattttctt tgttcttttc
     7081 gatcaggctt ttcgcttctt catatttttt atctgcacat agtgctttta cttcctgtaa
     7141 tacttttcct gcttccattt gcctcaccct cttgccattt tattgcttta agttttattt
     7201 aagttatacg cattattcta gtttcatccc aaataacctt ttttcatttt tactcctcga
     7261 actaatcagt tttctaaagg ctgattggtt cgtctttttt atttttcctc catttcattc
     7321 tgctcttctg ttaatttatc taatgctttg attgatgctg cggctcttgc ataatcatag
     7381 ctgtataatt ccataaattt atcaggcaga acaaaccacg ctggatgata accgttagct
     7441 gtgacaggga atcctttatc ttgttggtta tctccatccc atctcagagc aacacctttt
     7501 tttcctttcc actttactaa agcgatcgag tatcccttct ttgtttgtgt gccgtcttca
     7561 ataattgcta aaatttctat tttttctttt ggcgcacgga cttcttgagg acgaaccatt
     7621 ttctattcct cccttatctc attacttcac gaccaacaca ctgcaagggg catgtgatac
     7681 gacataagaa gcggtcgatc caactaatgc ttgttgtata gcaccttttc ctgtcgctcc
     7741 cataacgatc aaatcaatat tttcttgtcc agggatgaca ttggcaatca aatttcttgg
     7801 atctccagtt tcaactattg cgtggatctc agcaatgccg tactctttgg catcattgat
     7861 ttttttcagc atggctactt ctgttctttg tttttcatct gcatagattt ttgaaaaggc
     7921 ataggcacta tgggtcagct caactttgtt gatgattgac acgataaata agtgggcttc
     7981 attgtccttt gctaagtcaa gggcttcagc gaacgctttg atcgattgtc ttgatccgtc
     8041 aactgctacc atgatgttct ggtatctttt actcattgga accgcctcct ttttctttta
     8101 ttgtaacatc tcaagcaaaa atacaaaaaa tatacgcaat atgtaaataa aatgatgaac
     8161 aaaagcaatt tctttcaagt tatttataca tttcttttct catcagaaca cctgttctaa
     8221 tcccattatt tctgcattat gtgaaattcc tcatcatttt tcacatcgag cgaacatcat
     8281 tctgttacaa aaaacgtgga tttgtcataa attgtcatgc ttcttttacc tatgaaatat
     8341 tttcacgtgt taaagtattc ctcgttgaac acaacaaata aaacttttta acgaataaag
     8401 gagatttttc atgacatcac ttaaaacttt actttttgga acaactttgg ctgccggcgc
     8461 tgcattcttc atgggaacga ctgctcatgc ggacgaagct tataccgttc aatcaggtga
     8521 tactctatca acgatttctc aaaaatacgt tggtgataac tccttgatca atgccattgc
     8581 agaatcaaat tc
//
LOCUS       DACDYI010000099         8229 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.099, whole genome shotgun sequence.
ACCESSION   DACDYI010000099 DACDYI010000000
VERSION     DACDYI010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.099"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            328..1251
                     /gene="truB"
                     /locus_tag="IWM52_002634"
     CDS             328..1251
                     /gene="truB"
                     /locus_tag="IWM52_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAP9974819.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1255..2196
                     /gene="ribF"
                     /locus_tag="IWM52_002635"
     CDS             1255..2196
                     /gene="ribF"
                     /locus_tag="IWM52_002635"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAP9974820.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2591..3376
                     /locus_tag="IWM52_002636"
     CDS             2591..3376
                     /locus_tag="IWM52_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9974821.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3389..4240
                     /locus_tag="IWM52_002637"
     CDS             3389..4240
                     /locus_tag="IWM52_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAP9974822.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4364..4816)
                     /locus_tag="IWM52_002638"
     CDS             complement(4364..4816)
                     /locus_tag="IWM52_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9974823.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(5281..5649)
                     /locus_tag="IWM52_002639"
     CDS             complement(5281..5649)
                     /locus_tag="IWM52_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAP9974824.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            5799..6281
                     /locus_tag="IWM52_002640"
     CDS             5799..6281
                     /locus_tag="IWM52_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAP9974825.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            6318..7328
                     /locus_tag="IWM52_002641"
     CDS             6318..7328
                     /locus_tag="IWM52_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAP9974826.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(7443..8156)
                     /locus_tag="IWM52_002642"
     CDS             complement(7443..8156)
                     /locus_tag="IWM52_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974827.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         2784 a         1337 c         1667 g         2441 t
ORIGIN      
        1 ttaagaaact gaaaaaataa ataaaaaaaa acggaaaaaa gactggaaga atggacagtc
       61 ttttttttcg tttttgggaa gtgaaaacca aactatttac ctgtaagcca cccataaatc
      121 caatcaaatg gtgcaaagaa aaaatcttcc attctattcc ctcctttaaa aaaaacataa
      181 catgatttct atttacgtga gcatgaattt tgttacgttt ttattgccga aaaataaaaa
      241 ccgttcatta tgatgtcaaa ttcgtcagta attgatgcaa tgtgttataa taattcagtt
      301 aaatcaaaaa tgaaatggag aaaagacatg gacgggttat taactttatg gaaagaacgc
      361 ggaatgacaa gtcatgattg cgtattcaaa ttaagaaaaa tcttacatac gaaaaaaatc
      421 ggacatgggg gtacgcttga cccagacgta gacggtgtgt tgcccatttg tattggaaaa
      481 gccacaaaag ttatcgaata tttgacagat tctgggaaaa tctatgtagg cgagataact
      541 ctaggtttca gtacaacgac ggaagatcga tccggagaac gggtagaaac aaaaccagta
      601 gatatgtctt tatctgaaga aaaaatagat gaagtgatgg cttcatttat tggtgagatc
      661 acccagatac caccaatgta ctcagcggta aaagtcaacg gtaagcggtt atatgaatat
      721 gcccgcaata atgaaacagt ggagcgtcct gttagaaagg ctcagattta ccgttttatc
      781 cggacaagtg aattgacaag agacgaagtg acaggaacca tttcttggcg cttcgaagta
      841 gaatgcggga aaggaaccta tgtccgcaca ttagctgtag atacaggcaa gaaacttggt
      901 taccctgcgc acatgtctga tctgacccga acagcaagtg ccggtatgtc ccagaaagat
      961 gctctgacat tagaagaagt agctcaagct gtgaaaaatg ggacaatcga tgattgtttg
     1021 ctgccaatag aaaaaggagt gaaacgattc aaacgagtag atattactga tgaagtatgg
     1081 cagaaagtaa aaaacggtat gagacttaat tatactgtat ttgcgctccc agaaatgccg
     1141 tcagaagaaa ttgctttgtt ttataaaggc aaagttgtca gtatctatca gccaaacccg
     1201 aaagaaaaaa acaaattaaa acctagtaaa gtattgagaa atgaggaata acgaatgaat
     1261 atcatcaaga tcagacatcc ttaccaatca gaacaaattc cagaagacga tgtcgtttta
     1321 gtactaggat tttttgacgg cgtgcatatt ggacaccaga aagtcataaa gacaggtatg
     1381 gaaattgcac aaaaagaagg attgaaatta gcattgatga ctttcaatca gcatccatcg
     1441 attgtcttta aaaagatcga tcctagcagc gtgaaatatt tgactagcct taagcaaaaa
     1501 gaagaaaaaa tggccgcttt gggtatagat tatttatatg aagttgattt tacttcttct
     1561 tttgctcatt tagcaccgca ggaatttgtt gatcaataca tcgtagggtt acatgcaaaa
     1621 tatgctgttt ccggttttga ttatacgtat ggaccaaaag acattgcaga tgtcgctcat
     1681 tttcctgaat atgccaaagg aagattcaaa atcgtcacag tttctaaaga agaagaacaa
     1741 ggtgaaaaaa tcagctctac ccgtattcga ggattactag acagcggaca agtaaaagaa
     1801 gcagcaaaac tattaggttg cacatatgag gtagaagggg tagttgtcca tggcgatgca
     1861 cgaggcagac tactaggatt ccctacagca aatgtcaaag taaagagcac agttcatttg
     1921 cctaaagaag gtgtttatgt atcggaaata aaaattgggg aaatttggta tccagccatg
     1981 ggttcaattg gacataatga tacattcggt gaaggacggg agctgactgt agaattgtat
     2041 attttggact ttaatcaaga tatctatggt gaacatgtcg cagtacgctg gaatcaattt
     2101 ttaagagatc aagtgaaatt ttctagtgta gaggaattga ttgaacaact caaagaggat
     2161 gaacaagcaa cagctgaata tttttctcag caataaaaca ttatgcataa aatctcttgt
     2221 ttatgtataa ctagcaattt caataaaatg ggtgataaat aagcccagga aaggggctgt
     2281 gacaaaagtc ttgtcacagc ctttttttcc aaataaatgg tttttcagaa gtagcttctt
     2341 cggaaataag ccgaaatttc acaaaaattt gagaagtaat ttttgtaaat tacttcttat
     2401 ttcttgaagc tggccacttc tgtcacaacc tctttcttta tagaaataaa aggacactag
     2461 caattcatga aaacgattgc taaagcgtat catttgcttt agactatata tcttggtgta
     2521 cactcacttt aagacggaaa taaaagttat ccgtaataac aggtttaggt atgaaaaagg
     2581 agtgggtact atgtataagg aattagaaaa caaagtcgca gtagtaactg gtggctcaaa
     2641 ggggattgga acagcgattg ctcaacgatt cgggaaagaa aaaatgaatg tagttgtcaa
     2701 ctataactcg gataaagaag gagcagaaaa agcagtcaaa gcaatcgaag aggcaggcgg
     2761 aaaagcagtc tctgtacaag cagatgtcag ttctgaagaa ggaatacagc gtttacttga
     2821 tacggcttta gaaaattttg ggacactgga cgtttggatt aataatgctg ggatggaaaa
     2881 tcagcatcca acccatgaac ttagccttga agattgggaa agagtattaa aagtcaattt
     2941 gactggcgtt tttatcggaa cgaaagcggc tttaaattac tttacagaga atgataaaaa
     3001 aggaaatatc atcaatatgt cttctgttca tgaacaaatc ccatggccaa cgtttgctca
     3061 ttacgctgct tcaaaaggcg gagtaaaact attcaccgaa acggtagcaa tggagtacgc
     3121 accaaaggga atcagaatca atagtatagg acctggtgct atccgtacac ctattaatgc
     3181 ggcaaaattt gatgatcctg aacaaaaaga actcttggaa agtatgatcc cactaggacg
     3241 cgtaggtgaa ccaaaagaaa tagcggcgac agctgcatgg ttggcttcag acgaatccag
     3301 ttatgttaca ggtattacac tatttgcaga tggtgggatg actttatatc cttctttcca
     3361 aggtggaaaa ggataagggt gaataaaaat gggtttattg attgcattga tcccggcagt
     3421 tgggtggggg atccagccac tgattttgaa aaagattggt ggcagaccaa caaatgagat
     3481 attaggaact ggtattggag ccgtgctcgt tggcttactt gttcaattat tcgcttctcc
     3541 agctggtatt tcagtgacta catttttgat tagtttactt tctggtgcat tctgggtcat
     3601 cggacaaaca ggacagtata catctttcaa cttgattggt gtttctaaaa cgatgccaat
     3661 ttcgactgca ttacagttag tcggaacctc attgattggt gtcttcgctt ttggagaatg
     3721 gtctggaagt atgggaaaaa tcattggtgc agcagcaatc attcttcttg tatttggttc
     3781 tgctttaacg gcaatcagtg atgatcctgg aaaaaaaggc ggtatcacaa aaggaatcag
     3841 tctattagca tcaacaagta ttggttattg ggtttacagt gcattgccca aactagttga
     3901 tgcaagcgga ctttccattt tctttccgca aatgatcggt gtctttttag gggcagtgat
     3961 ttatgtgatt ttccgaaaac caaccgcatt ttctgaagac aagagttgga aagcaacaat
     4021 agttggagcc atcttcagtg tgtcagcatt ggcttatatt ttttcagcaa atgaaaatgg
     4081 ggttgctact gcatatatca ttacacagct aaacgtagtt atctctacat taggcggtat
     4141 gtacatcctg catgaaaata agacaccaaa agaaaaaaaa ttaacgatta tcggattatt
     4201 gctgatcgta gtaggaagca tggtcacggt ctttatttga ggataaagat ggtaaaatct
     4261 catttattaa aaaaatattt gtttgaacca aacagtaagt acttataaaa aaatagcgct
     4321 agaatttcca acatctctgg aatttctagc gctttttaat tgtttaagat acaagtgaaa
     4381 tatctgtctg catcaataat ttttcattga tccattgact aaaatttacg acatgattga
     4441 agttaccacc gattagtatc tccgcatgat tgtctttata aagatcgtat tgtaaaagac
     4501 ggtctttttt atttgcgtaa acatcgttat ggtactggac taattgttct cggatttgat
     4561 tgattgctgt aaaatcacga tactgaaggt tgaaacgcat cgtgattggt gcatttagca
     4621 tgaatcgctg catttctttt gctgcttcct ctgcttgatc tccgtatagg aactcaaaaa
     4681 gattacgttc agaaaatttc cattgcccgc caacctttgt tccttttagt tttccttcat
     4741 gaagataatt tctgatagaa cgtgtagtaa cacctaattg ttcggctaat tcatttactg
     4801 agtaaaaatc tttcaatgtt caccctccta tgtgaatggt tctactgact tagttgatat
     4861 ttgcttaatc acgaaatgta gaaagaacga ttttaattaa ggtcattgta aaaatcaagt
     4921 cagaaaattc taacttttct ttctctaaag aaattcgttc tctcctaaag tgatattata
     4981 tttaaatata cctgaaaata atgggcagta ttatgaaaga tcactgatat tttctcgtat
     5041 tttttcctta ttcaaaagtt ttgcaggaaa attgctgaaa aaataatttt actaagtgga
     5101 aaattcaaag ccattcaatc agtgattttt ttcagcacaa gaaagactcc ttataaaaat
     5161 agcagatact ttgtcaatta agtgtttaac cagggatgat aaaaaggata ggattgaaca
     5221 aaacctgtat aagctgatga atagtttcct cgccagtaaa aggtttattc gtataaagct
     5281 ttatcgccaa aatttatcat gacctttttt atcttcaata ttaaatgaaa tcaattgttc
     5341 aatcaattcc cagttgatta cacttttcca aggaactttg atgacgttat ccgtatatac
     5401 gtagcctgct tcttggatat ttttttcaag ggcacgaatc gctgcttttt ccggtgcgat
     5461 agaaaaatga tttttagcaa aactaaatgc aataataaag gtaccatggt cagtaaacat
     5521 aggttggttc cattttacag ttgtgtctag ctggggatac gtttctgtta tcttagcaaa
     5581 aaggtctttg atcttttgtt gatgttcttt ttctttgatc tgattgatga tcggctgaaa
     5641 tttttccatt ctaaaacgcc tccattaagt ggtttgattt attttaccag aaaaacaata
     5701 gttatgtata aaaaacgaac aaaacacaag taatgttgta accgctatat ttatatgata
     5761 agatgtctca aataataaaa tagaaaagag acataaaaat gattgacttg gcaaagaaat
     5821 tcttacacga aaatatatat tttgagttgt atcatcatcg agccatttat acaaatgaag
     5881 atgctttggt aataaagaaa gaacaaggat ttacaggtac agagaccaaa agcttattct
     5941 taaaagataa aaaagataat aactacgtat atctgacttt tactacgaaa caaacggatt
     6001 tcaaagcatt agggaagatc gtcggtaaaa ggctttctat tgtttcatca gaacgcatgg
     6061 aagaacaaac gggacaaaag cagggcgctg tttctccgtt tggttatgaa aaacagattc
     6121 cgattattat tgatgaagag ctcttatcaa atgaaaaact tgtttttgca ccaggtagac
     6181 cagatcaaac gatggtcatc caaacggttg acttaaacaa aatcatccag ctacttgaga
     6241 tgccgatatt tttgatgccg gcaaacgaaa aaaacaatta accaagtaaa atagtgcata
     6301 cttggaggtg aagcaacatg ataagttatc cattattaga aagaaatgca acgattgggg
     6361 ttactgcacc atcctcaggc gtatcagagg aacaccattc gttactcgaa tcagctttaa
     6421 agagaatgga agaaaaatct ttccaagtag ttactggaaa gacgctttgg acgcaagata
     6481 aggaaaaatc aggtccagca agaaaacgag cagatgaatt caatcaaatg atgcaagatg
     6541 aaaatatcca aatgatcttt ccgccttggg gaggagaatt attgattgag attctcgatt
     6601 ttgtcgattt tgatgctcta caaccaaaat ggatagtagg ttattcagat attagtacgt
     6661 tgctacttgc tgtcacatta aaaacaggga tagccactgc acatggaacc aaccttattg
     6721 atttaagagg agagtacttg gatgaaacga cagcaatgtg ggaaagtgta ctgaaaacaa
     6781 gagaaggaga atcgattcta cagcaacctt ctgaaaagta tcaaaaagag tggccccatg
     6841 agcattcatc atcttctgtt ttccatctca ctgaaccaac aaattggcaa tcagtcagcg
     6901 gagatgcagt aaggatgaag ggacgattat taggtggctg tattgatacg atcagtcatt
     6961 taatcggtac cccttatgga gatgtccaat cttttaaaag aaagtataat gatggaaagc
     7021 cgatcttgtg gtttctagaa aattgtgagc tatcgtcagt tgatttaaga agatcacttg
     7081 ttcagatgaa actggcagga tggtttgatg gcagctcggg gatcatgttc ggtagaagtg
     7141 cagcagagga tttgttagat tataaaatga aggatgtata tgaagatctg tcaaaggaat
     7201 taaaactacc gatcttgtat gatattgatt gtggtcatgt accgccacaa ctgacattga
     7261 tcaatggtgc ctatgcagaa gtagcggtag aagatagaaa agcgactgtt ttgcaaacgt
     7321 ttatttaaaa aattttttcc tgccttggag tttatcaaag accaacaaat ctaaatagaa
     7381 aagaaaatga aagaagctga cagtattttg ccagcttctt tttgtatgat ctattagaaa
     7441 ttttaattat ccataactat cacattgatc agctgtttca catcatttgg attacctgta
     7501 ggaatagcta ctacagatgc attatcaata atttcttgag ttgtttctct aatgaacgct
     7561 ggataagatg tttgatctgc aggcatttgc caatgtgaag gaactggata actagtgatt
     7621 gtcccgtcat ggttacttga atacactaaa actccctggt aactgtattt cccagtgagc
     7681 ataaccacat ctttcggata gactgcactc cctgcatcat aaggggcgat aggtgttcct
     7741 gcaggataat gaataacatg tagttcgaaa tcacccgatg tatctctaaa agtattacct
     7801 ctaattgcta gccaaacacg tgcgtactca atttgtagat cagaatatcc tgctaacgga
     7861 tctattgact gagaagactg tgaggaagtt gtgtctattg ttgaagaaga aatatttgtc
     7921 tcaactgtac tactagatgt agaagaagta ttgggctctg agactttact gcttgttgaa
     7981 gatgtattcg tttcagtaga agctgatttc tgagtacttg atttttcagc tgaactggtt
     8041 tgtttgattg aacttttgtc tgtggtcgca gatggatcac tattttcttt actaggagac
     8101 gtacatccta caaggaataa agagaccaac aataaaataa aacctaactt tttcatataa
     8161 aatacctcat tattttttta ataccagtat agcataatcc tgaatttttg aatccgtaaa
     8221 accttgtct
//
LOCUS       DACDYI010000100         7822 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.100, whole genome shotgun sequence.
ACCESSION   DACDYI010000100 DACDYI010000000
VERSION     DACDYI010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.100"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..344)
                     /locus_tag="IWM52_002643"
     CDS             complement(<1..344)
                     /locus_tag="IWM52_002643"
                     /inference="COORDINATES: protein motif:HMM:NF013753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9974828.1"
                     /translation="MKSLFKLLLKSEDRLDYNTLTKRRNFNWRLLTETEVVDRLLKIS
                     PELKTAYRYYQDLLTAYQEKDPDTFFQLLRAMPGEVPLELHHIKKAFFKYEKGIRLAL
                     LKKYSNARLENL"
     gene            complement(341..1180)
                     /locus_tag="IWM52_002644"
     CDS             complement(341..1180)
                     /locus_tag="IWM52_002644"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9974829.1"
                     /translation="MSMNHFIKQLFTIKDSNIQIQSFEETFYKSRKSIVIEGTLIRTY
                     RRCPCCKDSTKQIVKNGKKISMILLNRSGNKRTYLRLKKQRYLCRACKKYFTARTYLV
                     TPFCFISKQIHYKILEELTERQSIKAIGKHCDVSVTTVQRTLSALSSDIRQNLEYLPR
                     CLLFDEFRSLSTWHGKFSFSCMDGETGKLFEILPSRRKKDLVAFFMRFSLKARLGVHY
                     IVTDMNAPYFSLVKECFPNAQVIVDRFHIVQHLNRFFDAVRKRVMKTLDQKTQYKLNS
                     IAN"
     gene            complement(1359..2132)
                     /locus_tag="IWM52_002645"
                     /pseudo
     CDS             complement(1359..2132)
                     /locus_tag="IWM52_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            2153..3355
                     /locus_tag="IWM52_002646"
     CDS             2153..3355
                     /locus_tag="IWM52_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HAP9974830.1"
                     /translation="MCTKLLQNTAHKNPYKTVDEATQLDLVIVVNQLLTGYDSKWVNT
                     LYLDKSLEYQNIIQAFSRTNRLNGPEKPFGTIRYYRKAHTMEQNIEEAIELYSGKQAK
                     GLFVEKLYNNLEKLNDIFEEIKSVFNISGIENFSHLPEVPGEIKQFVNLYNQLIHCYE
                     SAMIQGFTWAKSTYEIQDYEPITVVITEYEFNILAQRYSEIEREPRDGNDSIAYDIDY
                     QLPEGSRSRINYEYLNTNFKKYMNIKEERDSNLIQSLLNDLHRTFAQLPVELQPYAEM
                     ILNDVNNGELVIKEGWEFTDYLNEYQLSEEDDFIQDLVDSTNINEALLREMLDLRLTE
                     VNINEYSRFDKLKSSVNVGHFSGYIEKNLGKMVIPIKVNMLIDRLLRAFLLEDVFDVT
                     EYIKNYFN"
     gene            4088..5056
                     /locus_tag="IWM52_002647"
     CDS             4088..5056
                     /locus_tag="IWM52_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9974831.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADARNHDLDVRRYMGKVEY"
     gene            5067..5882
                     /locus_tag="IWM52_002648"
     CDS             5067..5882
                     /locus_tag="IWM52_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAP9974832.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKKIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            5950..6660
                     /locus_tag="IWM52_002649"
     CDS             5950..6660
                     /locus_tag="IWM52_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9974833.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYVMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            6696..>7822
                     /locus_tag="IWM52_002650"
     CDS             6696..>7822
                     /locus_tag="IWM52_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAP9974834.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIK"
BASE COUNT         2598 a         1237 c         1589 g         2398 t
ORIGIN      
        1 tggagatttt ccagtcgtgc attcgaatac ttcttcagta atgccagccg aatccctttc
       61 tcgtatttga aaaaggcttt tttgatatgg tgcagttcca atggaacttc tccaggcatt
      121 gctcgaagca gctggaaaaa agtatctgga tctttttcct gatacgcagt aagcaggtct
      181 tggtagtaac gataagctgt tttaagttca ggagaaatct ttagaaggcg gtcaactact
      241 tctgtttcag tcaatagtct ccaattgaaa ttgcgtcttt tcgttagtgt attgtaatct
      301 aaacgatcct ctgattttaa aagtaatttg aataaggatt tcaattggcg atactgttta
      361 gcttgtattg ggtcttttga tcaagtgttt tcatcacacg ttttcgaacc gcatcgaaga
      421 agcggttcag atgttgaaca atgtgaaacc gatcgacgat cacttgtgca tttggaaaac
      481 actctttaac tagtgagaaa taaggagcgt tcatatccgt tacgatgtaa tgtacaccca
      541 acctcgcttt gagagaaaaa cgcatgaaaa aagcaacgag atctttttta cggcggctag
      601 gtaagatctc aaacagtttc ccggtctcac cgtccataca agaaaaagaa aatttaccgt
      661 gccatgtaga aagagaacga aactcatcga ataatagaca ccgaggtaaa tactcaagat
      721 tctgacggat atctgatgac aaggcggaca atgtccgctg aacagttgtc acagaaacat
      781 cgcaatgttt accaattgct tttatagatt gacgctctgt caattcttct aagatcttgt
      841 aatgaatctg cttagaaata aagcagaatg gggtaacgag atacgtccga gcggtaaaat
      901 attttttaca tgctcggcag aggtatcgct gtttcttcaa ccgaagatag gtccgtttat
      961 taccgctacg attcaggaga atcatagaga tcttcttgcc atttttaacg atttgctttg
     1021 tagaatcttt acaacacgga caacgtctgt aggtacggat cagagttccc tcaataacga
     1081 tactttttct gcttttataa aaagtttctt caaaggattg aatttggata tttgagtctt
     1141 taatagtaaa gagttgtttg ataaaatgat tcatggacat acaagaattc ccccagaatg
     1201 atgttgtagg agactctatt ctaccggaaa ttttgtatgt ctttttgttt ttttacaaaa
     1261 aaactaggac agatagaaaa tttccatcag tcctagaaat tatagagcca ataaaactta
     1321 gtccgatatg cggcgcgttc tgtgcgccgc atattttttt agttttcaaa gatcagacct
     1381 tgttgtaaat agatccgtgc ccgaaagtta taaaagttgc gataaccaaa cgcaattcgt
     1441 ttaatcagct tgatcttgtt atacagcccc tcagtgacgc cgtttgagtg ggacacctta
     1501 aaggcatttg aaatacccga ttggtagcga tggaagatcg tgaatttctt tttaaacgac
     1561 tgaggtaacg tagatgaaat tgttttaagg gtttcttgaa agcctaccgt atctcgttta
     1621 acgaaataat agcgtaataa ttgacaaaaa tgatacgcag ttcgcaattc ttcgttgtag
     1681 cttaacagct cattaacgat ttctgtttga ctaattggtc gtttaaacag tcgatgataa
     1741 tgccgtttga gtgcctccca tgtcgtggaa tctttgagta agagtttcca atatcgcttg
     1801 agccgacggt acttcttctg attttcagag tgtgaggttc gaaaacgatt catgatctgg
     1861 acacgtaatt gattgaagct gcgattgatg tgttggacaa tatgaaagcg gtcagttacg
     1921 atctccgcat ttggaaagac cgttttaagc aactgacaat agctggcatt catgtccatc
     1981 actaggtact tgacctttaa acgggccttt ctaggaaagc gcaggaagta ttgagttagt
     2041 ttgtaaagtc tgcgatcttc aagggtcgta aatagctgat tggttgtccc atcaacacaa
     2101 ataaaactca tggaaccaga agtggcaaca ttgtgtactt ttctttgcaa tcttgtgtac
     2161 aaaactattg caaaacacag cgcataagaa tccttacaaa acagtagatg aagcaaccca
     2221 attagatctt gttatagttg ttaatcagct cttaacgggt tacgattcaa aatgggtcaa
     2281 caccctatac ttagataagt cacttgaata tcagaacata attcaggctt tttcacgcac
     2341 aaatcgtctt aatggtcctg aaaaaccgtt cggaaccatt cgttattatc gtaaagcaca
     2401 tacgatggaa cagaacattg aagaggctat tgagttatac tcaggcaaac aagcaaaagg
     2461 gttatttgtt gaaaaattat ataataactt agaaaagttg aacgatatat tcgaagaaat
     2521 taagtccgta tttaatattt ctggcataga aaacttcagt catttaccgg aggttccagg
     2581 ggaaattaag caatttgtaa atctttataa tcaactaatc cattgttatg aatcagcgat
     2641 gatacaggga ttcacatggg caaaatcgac ttatgaaatc caggattatg aacctataac
     2701 agtagtaatc acagaatatg aatttaatat cttggcacaa cgttacagcg agatagagag
     2761 agaacctaga gacggaaatg atagtatagc gtatgacatt gattaccaat tacctgaagg
     2821 tagtcgtagt cgtattaatt atgagtattt aaacacaaac ttcaaaaaat acatgaatat
     2881 aaaagaagaa cgtgattcga atttaattca atcattgtta aacgacttac atcgcacatt
     2941 tgcgcaatta ccagtggagt tacagccata tgctgagatg attctaaatg atgtaaataa
     3001 tggggaatta gttatcaaag aaggatggga atttactgat tacctcaatg aatatcagct
     3061 tagtgaagag gacgatttta tccaagattt agtggattca accaatatta atgaggcatt
     3121 attacgtgag atgttggatt tacgcttaac cgaggtaaac attaatgaat atagtcgatt
     3181 tgataaatta aaatcaagtg ttaacgtagg tcatttcagt ggatatatcg agaaaaattt
     3241 aggtaaaatg gtaataccaa tcaaggtaaa tatgctaatt gacagattat tgcgtgcatt
     3301 tttattagaa gatgtattcg atgttacaga gtacataaag aattacttta actagagtac
     3361 acctacatct tatagtacat aaaaaaattc atctttttta gtttaataat tcaccaaaaa
     3421 ggtgagtaat gccatttttg aaaattgagc ttgttttatg tctacatctg tatccgtata
     3481 taatccaaag gattataaac tcagaagagt ctttattttt tctgaatata actactgtat
     3541 caaaaacaat cgattatgag acaaaacaaa atgacctctt ttatttaaga aaggaggtca
     3601 ttttttgtat cataaattgt attaaaatca atgtatcact ttttaatcaa ttttaagacc
     3661 ataaattgcg tactaaaaga aattctgcaa ttcaaaataa agcattttaa gccattttca
     3721 aatttaagtg cacccatcta cccgtcaaat cactttattt tccactccag tagtttccta
     3781 attcaactgt gtgcaaaacg atttcttcta aacaaatcaa gacaaactta taaaccttca
     3841 tcccagcacc ataattattt aaatcatagg catttttcga aattagtata tgtactcaag
     3901 tttcactgca gattatttat attttttaga gaaataaatt tttcctattg acaaacacaa
     3961 agaaagcgga tacaatacaa ttatgtcaca tgacgttata tgacgttatg taatatcttt
     4021 acatttaatc agttactttt attttggata aaattaacat ttttaaaatt ttaaggagga
     4081 aatgttgatg gatgtagcaa aaacggttaa cgcaattgta gaaaaaaatg atggagagat
     4141 ttctggaatt tatttggtgg cttgtggcgg ttcgctggta gatatgtatg tctcagatta
     4201 ttttcttaaa gccgaagcat cgaagtgtat tacaggctta tatacagcga acgaatttaa
     4261 ccatgtaacc ccaaagcaat tagatgataa atctgttgtc attctatgtt ctcatggagg
     4321 aaatacagca gaaacggtaa aagcaggaga agtcgctcaa gctagaggag ctgtgactgt
     4381 tggtctgact cataataaaa ctgcggattt attaaaggtt tctgatcata gtttccttta
     4441 cgaatgggga gatgatgcaa aaatagtcaa taatcctatg gccatcattc ttgatttaac
     4501 tgtatctttg gtcaatgtta ccgaaacatt tgaacattac ggtaaattca gagaaggcat
     4561 gaaggttatc gataccgtaa ttgaaaatgg cattaagcag gtgcaggatc gtattaaagt
     4621 gtttgctgat aagtacaaag atgaaacaat gttttatatc ttaggtagtg gaccatcata
     4681 tggacatgct tatggttttt caatttgctc tttgatggaa atgcaatggc ttgattcaac
     4741 ttctgttcat tctggagaat tcttccatgg accttttgaa gtaacagaca aagatacgaa
     4801 ttttattctc ttgatgagca ttggacgcac tcgtgcttta gatgaacggg caaaacgatt
     4861 ccttgataaa tatgcagaaa gagtagaaat cgttgatgca aaggaactag gattggatat
     4921 gatatcagat gaagtttctg aattcttcaa tcccatttta ttctacagta ttctttcaga
     4981 atatcgttca gctttagcag atgcacgcaa tcatgattta gatgttcgtc gctatatggg
     5041 gaaagtggag tactaggagg attaaaatga aggtattagg cttaggtgat aacgtcgtcg
     5101 ataaatatga aaatctggga attatgtatc ctggaggaaa ttcattgaat tttgcagttt
     5161 ttgcaaaaaa actgggacat gaaagtagct ttatgggagt ttttggctca gatgaggagg
     5221 cagctcatgt tttatcagca ataaaaaaaa taggactgga caacagtcac tctcgctttg
     5281 aaaaaggcga gaatggctgt gccagagtga agattgatga gggcgaccgg attttccttg
     5341 gcagcaattc gggaggtgtt actcgtgaat acccgattca gcttactgaa gaagacagag
     5401 aatatttaaa ccagtttgaa ttgattcata tgggattata tagccatgtc aatcatctat
     5461 tagaagagtt acaaaatgtt tctggaaaga tttcttatga tttttctgac gactttacgg
     5521 aagaagaggt tcagcgggca attgataaag ttgattttgg cttcttttct atagaagact
     5581 tttccgatga agaagtaaag cagtttttga tgaagcattt ttgtgagcga aatgatgtat
     5641 tgattgcaac ccgaggagaa aaaacggcta ttggttatga tggagcaaag ttttatgaag
     5701 tgaaccccgt gctagaagaa ccagtcgata caatggcagc gggagattct ttcttaacag
     5761 ctttcctact ttattttctc gaaggggacg atatagtcac cgcgatgaaa aaggggaatg
     5821 aatttgctgg gaagtcttgt ctggttgacg gttcgttcgg ctttggtatt cattacgatt
     5881 gagcttgttt taaattcctg catttgataa cctaaagaaa gaaatcccgg taaagtggag
     5941 gttaaaaaaa tgctggatac aaatgcacaa ttaccattgt acttacaatt aaaagaaata
     6001 ttaaaaaata aaatcaatac aggggagtat cccgagaata cgaaaattcc cgctgaatca
     6061 gagctgatag aaagttatga ggtcagccga atcacagtga gaaaagcagt agaagaactg
     6121 gtagaagaag ggtacctact taaaaaacaa gggaaaggaa cttttgtcaa ccggcataaa
     6181 gtttttcgta aaattgaata tgttatgggt ttcagtgaga gttgtaaatc taatggtttt
     6241 acaccgacta gcattgtttt ggaacgcaag atcattccag caactgaagg gatggcaaaa
     6301 aaactcgata tcgctcaagg tgatgatgtt ttatatatcc agagaaagcg aatggcggat
     6361 aacatcccca ttatgttaga aaacaattat ttttcttatg aaagatatag tttcttaatg
     6421 gaggaagagc tgactgattc tttgtatgaa gtacttaaag tccaaggtac cttagccatc
     6481 aatcctgggg aaactgcact tgaaattgtc acagctgatg accaaattgc caaaataatg
     6541 gaagtagctg ttgggacgcc ctttttcttt atggatactt taatcaacga tcaaaacaac
     6601 ataccaattc atgtaggtaa tcaatattac ctgggagaag tatatcgttt ttacttatga
     6661 tttcctgttt tgatttcaag gagtagattt tatttatgaa gcaaatattg cggtcaaaaa
     6721 tgatttttga tggagagcag ccacctttca aaggttatat tgtaatcaat gaagggatca
     6781 ttgaaaaggt ggaaaacaac tggaattaca gtgatctatt aaatgaaaac acggaattaa
     6841 ttgcttatga tgatcaattc gttatgccag caattcatga taatcatgta tttttcagtg
     6901 gttatttggc tatgaatgct ggcatagatt taagcgatac atcttcagct aacgaggctg
     6961 tagagcgtat taaagctgta atgaaccata aaaaagcaga tgaaccaatt tatgctcatg
     7021 gctgggatgc tgccctgtgg aaaactgacc catcagaaga gctattgaat gaaataaacc
     7081 actttggtcc aatcacagcg atcgacaaaa atcgcagcca ttgttggatg aatgctattg
     7141 ctaagcagac ttatggtttt acagaaaaag aaaccagtgc tgaaagtcga gtattattaa
     7201 ttaaggaaat gctagcaaac aaaaagcttt tagcagaggt ttacgctgaa tttgaagcat
     7261 tactgctttc aaaaggtgtt atatcaatga aagaaatcgt ttttgatgac gcagaatttc
     7321 tttctaatat acctcagagg aaaatactta gtaacctata tgtgcaggca gtccaagaac
     7381 ctctacagca tgaacttcta tatgagtata aggaaaaagt tttccctgaa aaggtccgct
     7441 ttggtggagt gaagatcatg gtagatggtg ttgttgcgga tagaagtgga gatatttacg
     7501 gtgtttatca gtcaggagta gcttcacccg agattgacta tgaggcaatt aaagcagaag
     7561 ttgctgcatt caatgaagta gagattcctt gctgtttgac aacggaagga gataaagcgg
     7621 gttataaagc tgcgtcaatt cttgtcgaat ttggaaagcg gttacctgaa ggcgttttta
     7681 attcgattag tgatttggaa atggttactc aaaagacagc agaaaaaatg aacgaaggac
     7741 aagtagtagc agaggtatat ccacaaattc tcggcttgaa tcaaacgtta gaagaggctt
     7801 acatgtcaac tgttataaaa aa
//
LOCUS       DACDYI010000101         7536 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.102, whole genome shotgun sequence.
ACCESSION   DACDYI010000101 DACDYI010000000
VERSION     DACDYI010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.102"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(74..364)
                     /locus_tag="IWM52_002651"
     CDS             complement(74..364)
                     /locus_tag="IWM52_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974835.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(482..742)
                     /locus_tag="IWM52_002652"
     CDS             complement(482..742)
                     /locus_tag="IWM52_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974836.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(879..1292)
                     /locus_tag="IWM52_002653"
     CDS             complement(879..1292)
                     /locus_tag="IWM52_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAP9974837.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1748..2233)
                     /locus_tag="IWM52_002654"
     CDS             complement(1748..2233)
                     /locus_tag="IWM52_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974838.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2372..2587)
                     /locus_tag="IWM52_002655"
     CDS             complement(2372..2587)
                     /locus_tag="IWM52_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAP9974839.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2811..3611
                     /locus_tag="IWM52_002656"
     CDS             2811..3611
                     /locus_tag="IWM52_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9974840.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3630..5498
                     /locus_tag="IWM52_002657"
     CDS             3630..5498
                     /locus_tag="IWM52_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9974841.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5491..5982
                     /locus_tag="IWM52_002658"
     CDS             5491..5982
                     /locus_tag="IWM52_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP9974842.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            5970..6524
                     /gene="srlA"
                     /locus_tag="IWM52_002659"
     CDS             5970..6524
                     /gene="srlA"
                     /locus_tag="IWM52_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAP9974843.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6542..>7536
                     /locus_tag="IWM52_002660"
     CDS             6542..>7536
                     /locus_tag="IWM52_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAP9974844.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2684 a         1208 c         1433 g         2211 t
ORIGIN      
        1 aaaaaagata acacccaact ttcaaaaata tgttttggaa gttgggacaa acgagttaat
       61 acattttaat acattatttt ttattcaaaa aaaatagcac aatggcaaag atcgtaagag
      121 agatcataag ccccaatgaa tgactaaaaa gaaaagcaga atcacatgcc atatcatccc
      181 atatttcttc aagaaaataa ataagcggga aaaagggaag gaatagtaga acatcgatcc
      241 agcaccattt ttttcttctt ttgaaataac ataaaacaat aaacatgaac gtgcagggaa
      301 gtaaaaacaa agctttactc ataactaaca aaataccagc tccttttatt tttttctccc
      361 ccacattcaa agagtaacaa tggtatgtac ttaataatag aaaaatggat caacttgtat
      421 gaataacagc tgaaaagatc tgcaccaaat aaaaaacttc cagccaaaac tgactggaag
      481 tttatttttt tctgaaatat tctaacacaa atatgaaaaa gatggccgaa gctaataagc
      541 cgatttcgta gcaaaacaat gagataccag agtcgtaata ataaaatcct ctaatcttat
      601 ggataaaata gatcatcgga caagcagata agagtaagta tttgttaggc cttttgaata
      661 agtaaaagaa agcactggtc attatgataa atggtatcca aaacaaaagt ttacttaaga
      721 caatcaaatg atcaactccc atatttttta ccaagagaga tcataacatc tttatcgtaa
      781 aaacaaaata tttttttgct cattttgaaa atttaaacat actcgttctt atccaaatat
      841 ataaaaatcg cccctcattc atcgagaggc gataagttct aatttaactc aatttttttt
      901 gtttcttttg gctgataagc tgttttaggt aataccaatt tcagcacacc gtttttaaag
      961 gttccagtga ttttttcttc atcaacatta ggaagataga atgaacgttt gtaagagacc
     1021 gaatgtcttt ctttccgtac atagttgcca tcttctttat cttctgtatg ggactcatga
     1081 ttggcggaga tcgtcaaagt attatctgaa tattcaactg tcgtgtcttc tttgtcacag
     1141 ccaggcatat cagcagttag tttgtattct ttttcatttt cgactaaatc tacttttgga
     1201 taacttgaaa cacctaaaaa atcattgaat gctggactaa acacgtcatt gaaatcaggg
     1261 aacatgtcgc gaatgctagg tacatttgcc atttttgatc atcctttcaa taagtatttt
     1321 ttatttatcc ctatttacgt aataactcta ttttgaaaaa aatgcaagta taatggttag
     1381 ctttgtgaat atttcatcta atacgataca aaggctatct ttctaatttg aaaaagggat
     1441 cattaaaata ataagaagaa aatatgtcaa aaggtaaaaa agtaaattct tctatccaaa
     1501 tatagaaaaa ttgaatctag aaaaccgccc gacaataaat ttcattaact cagttgaccg
     1561 aaaatgaagt agataccaaa aatttatcaa gagagcaagt agagaagtga gtatattcat
     1621 aagagaaata gctgtaacga attattaggt taatgcaaat gcttatttat actccgtata
     1681 actttctcag aaaaaatgat accaataatg acttgctagt actgtaaatc cgtataatat
     1741 catctaatca aaatggtgtt ccatttgcat ctgtcatcgt aatcgatcct tcgggtgaca
     1801 ctcgataaaa accaacgctt ccagatcctc cttgggaacg gatttgtttg ctggaagctg
     1861 tgaacaaata atctcgatca attttttgat aaaagctaat aacaatatcg tcactttgta
     1921 attcggaacg gtgggcaata agaaatagaa gtgctttgtc agagttttct aatggaagag
     1981 aaggtacagt gctttcttgc atttcagatg ttgaagaaat gctaaacgac tgaatacttg
     2041 attggctatg ttgcgaatct acattaccag tagaagtatt agtaatggaa gtagtagaag
     2101 tacttttagt tgaaccaacc tcagttgttg aaatggctgc tgaagattct ttaacagaat
     2161 tctttgtttt atatccacag gcagaaaaaa gtattacagt tcccaacaag cctgcgtaaa
     2221 tccctttatg catgatagct cctcctaaaa tagattatct ttattcagta tatcacttaa
     2281 acaaagaaaa gcagatggtt taaaatgaaa aaaccttccc aataaaggga aggttcatga
     2341 agataataac ttttttaata attatagctt tctaatatct ttcatcaact tttctagtaa
     2401 tcattgggaa aaatttcaag cagtagatcg cacaaatacc tacgacgata taaatgatct
     2461 tcgcaaaaat agttgttgaa ccaccagcaa tcatagctac taaatcaagt tcgaacaagc
     2521 ctaccaacag ccagttcaac cctcccacaa tcaataaagt taatgcaata gcatctaaag
     2581 ttttcattag aattctcctt tcttaaatga taaaacaatt atatatcaaa aagagaaaca
     2641 ttgcttatat aacgctcagg aattttagca atctatgcaa aaagatgaaa attttttttg
     2701 ctcttccatt agtacacatt ttttagctga agctgtaaat ttcatgtctt aatttttttt
     2761 agtgagcttt tatactggat ttgtaagaaa aaagaaggag aggatcacat atgtcggatt
     2821 ggttggatat tgcaggaaaa gtagtaattg taactggagg ttcctcagga attggaagaa
     2881 gtatcgtcga aaatttgttg aagcagaatg cacaagtagc gaattttgat gtaactgaat
     2941 gccgtataca gcatgaaaac ctactatctt taaaagtcga tgtgtcttct aaaacggaca
     3001 tagaagaagg gatatataaa gtaatgaaac atttcaagac gatcgatggt ctagtcaata
     3061 atgcaggaat aaatattcct tctcttctaa tagatagaaa tcatccaaaa agcaagtatg
     3121 aattatcaga acaagttttt gataagatga tcgctgtcaa tcaaaaaagt gtatatttga
     3181 tgagccaagc agttggaaga atcttggtgc aaaagggatc aggagtgatc gtgaatcttt
     3241 cttctgaatc aggattggaa ggatcagaag gacagagctg ttatgctgct acaaaagcag
     3301 cgatgaacag ttttactcgt tcttgggcaa aagagttagg aaagaggaat gttcgtgtgg
     3361 taggtgttgc accagggata ctggaagaaa ccggtttgag aacacaagaa tatgaagaag
     3421 ctctttctta tacgagaggt ataagcgtcg aacagctgag aaacggttac agtcgaacaa
     3481 gtacgatccc tctaggaagg agtggtaagc tgcaagaagt ggcggatcta gtctgctatt
     3541 atctttcaga aagatcaagt tatatcactg gagtcacgac taatatttca ggaggtaaaa
     3601 caagaggata aaggataggg gggacgcacg tgacgctagt aaacagatgg taccaattat
     3661 tgaaattact agtcactcac aagaaagttt caatccagga agtgcaaaaa gaaatcaaaa
     3721 ccagtaatca aaccataaaa aaagatattg gggagttgaa tgaagaaatt gccggtattg
     3781 caaaaatcat ccaaaaaaac aaacattttg aacttgaaat ttatgatttt gatttttttg
     3841 atgagatcat gcaaggaaaa ttgaaaacag ccagtgattt caattcggca tccaaaagaa
     3901 tcgcttattt actaaatagg ctgatcgaaa cagaggattt cttacgtatg gatgatttgt
     3961 ctgaagaagt cggtgtcagc agaggaacag tggtcaagga tctaaagaca ttaaaagaga
     4021 tgatcaatga ttttgatgta aagattacag gcacaccaaa taaaggacta agaataatag
     4081 gagatgagct agaattacga ctacttttat tttattttgt ttctccctac ttttcagaga
     4141 cgaaaacaga acagtttcta gaagaaaaac aagcaatcaa acattttcag aagaagacga
     4201 atgctagtaa acaagaaatc acccttttaa cgaaagttat cggtatttct ttgaatagaa
     4261 tctctagtaa ctatctgttg aagaagccca tcaatcgtta cagtgggtgt tttgaatata
     4321 tccaagaatt caatgaactg atcaatcagt tggaagaaat ctatgaacta actttgagcc
     4381 aatatgaaaa agattttatc agttttcctt tgaacatcat caacacaagt ataacgatga
     4441 taaggaaagc taactctgat ttgcgcttat atttcgaccc catgatgatg cgtattcgac
     4501 gattagatct cattgatctg gatgagtcct tcttatatca agagatgaaa cagcacctct
     4561 ctttattgat aaatcgggta atttttcgtt atcgattgac tgatttattt tatggtgaga
     4621 tagaaaaaaa gtatcctttt tcttaccaaa tcgctagcga atgcatacaa gcattagaag
     4681 ctatcttgca acataagatc tctaaagtag agatcagcta cctaacgttg tatttggagt
     4741 tagcatttcg atatcaaaaa gagcattttt cctctaaaaa agtagccatt gttcatacga
     4801 caggaaaagg aacagcatta atgatccaaa gacaaatcaa aaggatattg gggagggata
     4861 tacaaataac acatgtttca gaagaacaat atgaaaaagt agatctaaac aaatatttcg
     4921 ctgtttttac aacgattccg ttaaaaaatg taaatgaaga gacaccggtg atccatttga
     4981 ctaatctttt tgatgactct tggttaaggg cacaatggca aaaagcaaaa aaaaagcgat
     5041 tagccgggtt tcattctact gtttttttct atcaaatatg gaaagaaaaa aagacgtatc
     5101 aagaatattt aaacaaatta gtcgctcctt tgatcagagg ctattatgtg gatgaagaat
     5161 ttgaaaaaag gatcttgaaa agagaatatc agcaaagcac atttgttaac aaagaatttg
     5221 cttttcctca cacaaccaac cagctatctc aacacattgt tttatcagta gggctaataa
     5281 ctgatcaagg tttagcagaa acaggaggaa atggagttat ttttttattt gccatacctg
     5341 aagaaatgac ggctgaagtt gagaatgaat tatttgagct atacgatatc ctatttgaac
     5401 tcataaaaat gggaatagat gagtttattg aattagcacc gacacaagaa tcatttttaa
     5461 aactaatcaa aagtaaagga gagagacttc atgaatgata tgtatctatt catagttttc
     5521 gcagcttcag cttatgtgct gcaagcagtt atcagttgga agcaactaaa gcatttcaat
     5581 caaacatata gcgaactgcg aagaaaagga aaagtagcga ttggtaaacg acctgggaaa
     5641 atccgttcag gcacgattgt tatgctagct atagacgatc atgatcaagt tttagaagtc
     5701 agaaaaatgc aaggggttac atctttggca aaatttcatt cattacctgt ctatgcgggt
     5761 gaagatatcc attacatcga tcgttaccat ccctcagttc gagcggaaga tcgtttgaca
     5821 caagcagctt tagaaaatgc cagagaagta tacttacgaa tagaggcagg aaatgaaaaa
     5881 agtgaacaac cttcattgcc ttttattgct atgctcaata attttcggat atggaaaagt
     5941 acgatcaaag aaaaaaggag tgacaaacga tggatatcat aacaaaattt gctgagggat
     6001 ttatgaggct gttccaaaca ggtgcagaaa cattcataag ctggatgaca ggaatcgtac
     6061 cagttgtctt gatgctgatg gttgcaatga atacgctgat tgctttttta ggtgaagacc
     6121 gagtaacgaa agtagccaaa ttatcagcga aaaatccgct tactcgttac cttgtgttac
     6181 cgttcttatc tgcttttatg ttgggaaatc ctatgtcatt tacaatggct cgtttccttc
     6241 cagaatacta caaaccaagc tattatgctg ctcaagctca attttgtcat acgagcaatg
     6301 gtgtattccc ccatattaat cctggagaat tgtttgtatg gatggggatt gctcaaggga
     6361 tcgaaacctt agggctaaat tcgatggagc tggctatccg atacttgctt gtgggactgc
     6421 tgatgaactt tattggtggt tggataactg attttactac tggatttgta tgcagacagc
     6481 aaggaatcgt tcttagcaaa aaagtagagc tttcagttga ttagagaaag gagagtttta
     6541 aatgtatggt gatattaaga tcgtaagagg aaacggcggg tatggaggtc ctttgattat
     6601 taggccgaca aaagataaac acaagttcat ctatgtcaca ggtggaggtg agaggccaat
     6661 gatcgtagat aaaatagaag aactgacagg tatggagtct atcaatgggt tcaaaacatc
     6721 aatacctgat agtgaaatcg cgcttgcaat cgttgactgt ggagggactc ttagatgtgg
     6781 aatttatcct aaaaaaggga taccaaccat caatatcgtt tccacaggaa aaagtggccc
     6841 attagcacaa tatatcactg aagatattta tgtttcagca gtagggttag aacaaataca
     6901 gccactaatg gaaactgatg ataacaaaga aacagtaaat actgcatctc cagataaaaa
     6961 gtacaaatat gatacttcta aaaagataac cgagcaaaaa gcagagcagc aaaactggat
     7021 agcaaaaatt ggaatgggcg cgggaaaagt agttgctaca ttcaatcaat cagcaagaga
     7081 agcagtagat acgatgatcc ggacagtgat cccttttatg gctttcgttt cccttttgat
     7141 tggtgtgata caaggttctg ggatcggtaa tgtatttgct aacattatgg cacctttagc
     7201 aggaaatgca gttggattaa caatcattgg ctttatttgt tcactgccat ttttatctcc
     7261 tttgcttggt ccaggggcag ttattagcca agtgattgga acattgatcg gtgtagagat
     7321 tgggaaagga aatattcctc cccaattagc attaccggca ctatttgcta tcaatactca
     7381 aaatgcatgt gattttattc cagtcggtct aggtttggaa gaagcaaaag ctgagacagt
     7441 agaagtaggt gtaccatcag ttctttattc aagatttctt aacggcatcc cacgagttat
     7501 cattgcttgg gtagcaagtt ttggattata tagctg
//
LOCUS       DACDYI010000102         7376 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.103, whole genome shotgun sequence.
ACCESSION   DACDYI010000102 DACDYI010000000
VERSION     DACDYI010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.103"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..165)
                     /locus_tag="IWM52_002661"
     CDS             complement(<1..165)
                     /locus_tag="IWM52_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9974845.1"
                     /translation="MSRRQRRTYSKEFKQQIVNLYLAGKPRAEIIREYELTPSSFDKW
                     MKQAQSTGSFK"
     gene            complement(367..762)
                     /locus_tag="IWM52_002662"
     CDS             complement(367..762)
                     /locus_tag="IWM52_002662"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HAP9974846.1"
                     /translation="MKVTMLKGKIHRATVEQAELDYVGSITVDMNLLEAAGIYEYEKV
                     QIVDVNNGNRFETYTIAGERGTGMICLNGAAARCVSTGDKIILMAYCELNENEVKDHR
                     PKVVFVDDNNKVERVTSYEKHGRLSDIIL"
     gene            complement(772..1620)
                     /locus_tag="IWM52_002663"
     CDS             complement(772..1620)
                     /locus_tag="IWM52_002663"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HAP9974847.1"
                     /translation="MVIFKTVNDVRSQVKEWKKQGLKVGLVPTMGYLHEGHESLIRKA
                     SKENDKVVVSIFVNPTQFGKNEDLGSYPRDLERDIEVCTRGRATAIFNPEVEEMYCDN
                     ASTFVNITGLTEGLCGASRPIHFRGVCTVVSKLFNIIPADRAYFGEKDAQQLAVIKRM
                     VRDLNIDIDVVGCPIIREEDGLAKSSRNTYLSLEERSSATILNKSLTLAKEALNNGER
                     DSLKIIEIISKNINTYNLAKIDYVEVVDSLSLQRVNYIEKSVLVAIAVFIGKTRLIDN
                     FTFELQ"
     gene            complement(1635..2462)
                     /gene="panB"
                     /locus_tag="IWM52_002664"
     CDS             complement(1635..2462)
                     /gene="panB"
                     /locus_tag="IWM52_002664"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HAP9974848.1"
                     /translation="MKNTAVTFKESKLRNEKLTMLTAYDYSTAKIIDEAGINGILVGD
                     SLGMVCLGHEDTLSVTMEDMIHHTRAVTRGAKNTLVVADMPFMSYQTSVYDSVVNAGR
                     LIKEGRAQVVKLEGGIEVCDKIEAIVKASIPVMAHIGLTPQSVNAFGGFKVQGKDKEA
                     AKELIRAAKAVEKAGAFAVVLECVPTKLAELISKEISIPTIGIGAGAGCDGQILVYQD
                     MLGMYSDFTPKFVKKYANLSEEMNKAFTKYIEEVKDGVFPGPEHGFKISDDVLEKLY"
     gene            complement(2474..3334)
                     /locus_tag="IWM52_002665"
     CDS             complement(2474..3334)
                     /locus_tag="IWM52_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HAP9974849.1"
                     /translation="MEVIIMNVGFIGAGKVGCSFGKYFQEHKIQVTGFYSKSEDSSLA
                     ASNFTSSKQYLNLRELVDENDTIFITTPDGQIQEVWQEIKNYQIKNKLICHCSGAISS
                     DIFSNIQDYGAYGYSVHPMFSISDKYNSYKKLKKSFITIEGDEKYAKFLCDFFKKLGN
                     STVIVSKENKSLYHAASVVSSNLVLGLINTSVTYLIQCGFTEKMAIEALYPLIEFNIR
                     NIKEKGIIESLTGPVERCDISTIKGHCEVLSDEDRALYMLLSKNVLEIAMLKNINRDY
                     SELEKYLGES"
     gene            3758..3952
                     /locus_tag="IWM52_002666"
     CDS             3758..3952
                     /locus_tag="IWM52_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974850.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            3942..4295
                     /gene="tnpB"
                     /locus_tag="IWM52_002667"
     CDS             3942..4295
                     /gene="tnpB"
                     /locus_tag="IWM52_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP9974851.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            4397..5944
                     /locus_tag="IWM52_002668"
     CDS             4397..5944
                     /locus_tag="IWM52_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP9974852.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKTDKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            6021..7094
                     /locus_tag="IWM52_002669"
                     /pseudo
     CDS             6021..7094
                     /locus_tag="IWM52_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT         2395 a         1365 c         1121 g         2495 t
ORIGIN      
        1 tttgaatgag cccgttgatt gtgcttgctt catccattta tcgaaagaag aaggcgtaag
       61 ctcatactct cgaataattt ctgcgcgagg cttaccagcg agatagagat tgacgatttg
      121 ttgtttgaat tcttttgaat aggttcttcg ttgacgtctt gacatgaaaa atcctccagt
      181 gtgtttttta ttattctaca caccttattt tttctgtcta gtctagtgta gccgattcac
      241 ataaaatgga ctttgtctac agtctgtatc ttctcttttt gtgtctactt tattgacatc
      301 tatccaatta gtaatcaata aatatttcaa taatagtcta gctatatata aataacatta
      361 caaaacctaa agaattatat ctgataacct tccatgtttt tcatatgatg ttactctttc
      421 aactttatta ttatcatcga caaaaacaac cttaggtcta tgatccttaa cctcattttc
      481 atttaattca caatatgcca ttaaaattat tttatctcca gttgatacac atctagctgc
      541 agctccattc aagcaaatca tccctgttcc tctctctcca gctattgtat aagtttcaaa
      601 tctatttcca ttattaacat caactatttg tactttttca tattcatata ttcctgctgc
      661 ttctaataaa ttcatatcaa cagtaatact tcctacataa tctaactcag cttgttcaac
      721 tgtagctcta tgtatttttc cttttaacat tgtaactttc attatagccc cctattgtaa
      781 ttcaaatgta aaattatcta ttaatcttgt ttttcctata aatactgcta ttgcaactaa
      841 aactgatttt tcaatataat ttactctttg taatgataaa gaatcaacaa cttctacata
      901 atcaatcttt gccaaattat aagtatttat atttttactt attatctcaa ttatttttaa
      961 gctgtctctc tctccattat ttaaagcttc cttagctaaa gttaaagatt tatttaaaat
     1021 agtagctgaa gatctttcct ctaaacttaa gtatgtgttt ctagaacttt tagctagtcc
     1081 atcctcttcc cttataattg gacaccctac aacgtctata tcaatattaa ggtccctaac
     1141 cattctttta attacagcta actgttgtgc atccttttct ccaaaataag ctctatctgc
     1201 tggtattatg ttaaataatt tacttacaac agtgcaaacc cctctaaaat gtattggcct
     1261 actagctcca cataaacctt ctgttaatcc agttatatta acaaaagtac ttgcattgtc
     1321 acaatacatc tcttcaactt caggattaaa tattgctgtt gctctacctc ttgtgcatac
     1381 ttctatatct ctttctaagt ctcttggata actacctaaa tcttcatttt tcccaaactg
     1441 agttggattt acaaagatac ttacaacaac tttatcattt tcttttgatg cttttctaat
     1501 taaactttca tgaccttcat gtaaatatcc catagtagga actaatccta cctttaagcc
     1561 ttgttttttc cactctttta cttgacttct aacatcattc actgttttaa atataaccat
     1621 atttcttgac ccttttaata taatttttct aaaacatcat cacttatttt aaacccatgt
     1681 tctggacctg gaaaaactcc atcttttact tcctctatat attttgtaaa tgctttattc
     1741 atctcttctg ataaattagc atatttcttc acaaatttag gggtaaaatc actatacatc
     1801 cccaacatat cttgatatac aagaatttgt ccatcacacc ctgcacctgc tccaatacca
     1861 attgtaggta ttgatatctc tttagaaatt aactctgcaa gcttagttgg aacacattct
     1921 aacactacag cgaatgctcc tgctttttct acagctttag cagctcttat taattcttta
     1981 gcagcttcct tgtcctttcc ttgtacctta aatcctccaa atgcattaac agattgaggt
     2041 gttaaaccta tatgcgccat aacaggtata gaagccttta ctatagcctc tattttatcg
     2101 catacttcta tccctccttc taatttaaca acctgagctc gtccttcttt tattaatctt
     2161 cctgcattaa ctactgaatc atatactgaa gtttgataag acataaatgg catatctgca
     2221 acaactaaag tgttcttagc tcctcttgta actgctctag tatgatgaat catgtcttcc
     2281 attgttactg aaagtgtatc ttcatgtcct agacatacca taccaagaga atctcctact
     2341 aatattccat ttatcccagc ttcatcaatt atttttgctg ttgaatagtc ataagctgta
     2401 agcattgtta atttttcatt tcttaattta gattctttaa atgtaactgc tgtatttttc
     2461 atctatttct ctcctaactc tctcctaaat acttttctaa ttcactataa tctctattaa
     2521 tattttttaa catagctatc tctaatacat tttttgataa tagcatatat aaagctctgt
     2581 cttcatctga taaaacttca cagtgacctt taattgttga aatatcacaa cgttcaactg
     2641 gccctgtcaa actttctata attccttttt cttttatatt tctaatatta aattcaatta
     2701 atggatataa agcttcaatt gccatttttt cagtaaatcc acattgaatt agatatgtaa
     2761 cgcttgtatt aataagtcct agcacaaggt tactagatac tactgatgcg gcatgataaa
     2821 gagatttatt ctccttagat acaattacag tactattacc taactttttg aagaaatcac
     2881 ataaaaactt tgcatatttt tcatcgcctt caattgttat aaaagatttt tttaattttt
     2941 tgtatgaatt atatttatct gatattgaaa acataggatg gacagaatag ccataagcac
     3001 catagtcctg tatatttgaa aagatatctg aagatattgc accactgcaa tggcagatta
     3061 atttgttttt gatttgataa tttttaattt cttgccatac ctcctgaatt tgaccatcag
     3121 gagtcgtaat gaaaatagta tcattttcat ctactaattc tcttaagttt aaatattgtt
     3181 tactagatgt aaaattcgaa gctgctaatg acgagtcttc gcttttacta taaaatcctg
     3241 ttacttgtat tttatgttcc tggaaatact tcccgaatga acaacctacc ttgccggctc
     3301 caataaaacc aacgttcata attatcacct ccataaagaa gtgagactac aagtcccatt
     3361 cttacgatat gagcttaaaa ataaaaatta aatcaaatat acagtatgtt tttttcaaat
     3421 atcatccata gttaatttat cataatatcc catattttaa aataaaagat agcgtaaaat
     3481 attttgtgta aatagaaatt tagggtagaa aaaaaagtcc attctgtaaa attaaagtta
     3541 ccacacagaa attttatagg aggactttct catgactaaa tttactatag aaattatgca
     3601 aacactactc aataaagggg atttagatga attatttcag gatcatttag agcgagcgat
     3661 taattcactt ctacagtaag cgccccatag aacagtacct tactaacaaa tgcacctcaa
     3721 gatatacttg agctatcaag aagtctggag gttttctatg tataaattaa atgagattgt
     3781 tcctgtccaa ttaggaaacg aaaagagtgc accagaaaaa ggtaaacccg cggctgccaa
     3841 gttagcgatg cgcatcaaaa gagagaatgg ttctgaagtt ttgatctata acggtattaa
     3901 caattatatt cttcaagcta ttttgaaaga actgttgtcc aatgctttat gatttacttc
     3961 aaaccaaaca tatttatatt gtgtgcggaa aaacagattt gcgaaaagga attgatggtc
     4021 ttgccagtct tattcaacaa gagtatcaac tcgagcttta cgaagatgcc gtttttttat
     4081 tttgcgggaa ccgtcaagac cgtttcaaac ttctctattg ggacggtgat ggttttcttc
     4141 tctgttataa gcggatagaa aacggcaagt taaaatggcc tcggacgaaa gacgaagttc
     4201 gaaccttaac gaaccaacag gttcgatggt tgttagaagg attaagtatc gatcaacctc
     4261 gagctatttt gccaggaaaa aaaggagttt tttaagctgg ggggttaaca caaaaaatgt
     4321 gttgaacccc tatttcttgt gcgtttcttg tggtattctg taacaaaatc agttcgattc
     4381 aaggtggtga aaaattttgg aaactacaga tacgttgctt cagttgcttc aagaagccca
     4441 taaaaccaat caagcacaac aacaaaccat tcagaatctt acaactgaaa tccaactatt
     4501 gaatgaaaag gtcaattatt taaccaacaa attatttgga cgctctaaag agactctctt
     4561 tgaagaaacg aatgggcaat tgaatctttt tagtgatgaa gaaatttctg taagtgttcc
     4621 agaagcagct gccaccatta ttccagtaaa aggacatcaa cgagttgtag gaacgaaaac
     4681 agacaaaatc aaacacttac ctataacaga gaaagaacat ctccttcctt tggaagaaca
     4741 attttgtgaa cattgcggtt cacagatgaa agatatcgga cggacaaaag tacgagaaga
     4801 aattcgtttc catcaggcta tgttggattg tctcacccat tatcaacata cctattgttg
     4861 taaaagctgc gaaaaagaag gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt
     4921 gatttctaat agcttaggtt ccaatagttt agtggcggaa acgatccgga tgaaatttgg
     4981 tcagaaagtt cctgcctatc ggcaagagaa ctattggaaa caaacacatg ggttggatat
     5041 ttcgagagat aatatcacca attggcatat taaagcagtg caaaacgcgt tagacccttt
     5101 aggagaacga ttaagagtct atcttaatca agaagagatt ctacacgggg atgaaacaag
     5161 ttatcgtgtg attgaaagtg ccaaaacaga tacctattac tggcagtttt gtacaggaaa
     5221 agacagtcag catcccatcg tttattatca ccacgacgaa agtcgtgctg gagatgttcc
     5281 gaaaactttt ctcaaggagt ttacagggta tttacactgt gacggctata gcgggtataa
     5341 tgccgtggaa agtgtacgac ttgtctattg tttcgcgcat gttcggcgga aatttttcga
     5401 agcaattcca aaaggaaaga aaaacacgga tattcccgca gctcaagcag tcaaacaatt
     5461 ggataaatgg tttgtcctag aaaagaaatg gaaagacttt tctcctgaga agcgattaag
     5521 ctgtcggcaa caagaactgc gtccattatt tattgctttt tatgagtgga tggcaacgat
     5581 agatcctgtt gcaaaatcca agttggatgc agccgttcaa tatgcgtgta aactacgaag
     5641 tggttttgaa cctatttttg aagatggtcg tttagaactc acaaataatc gtgcggagcg
     5701 aaatatcaaa gagctggtga taggacggaa aaattggttg cattccacaa gtcttgaagg
     5761 agcacgaact tcaggaatca ttctgagtgt atataaaaca gcagaactta acggattgaa
     5821 tcctgtaaag tatctagagt ttctatttga taagatacca aatcttcccg tactttcagc
     5881 agaaacttta gatcaactgt tgccttggaa caaggatgta caacaacatt tttctcgtaa
     5941 ctaaaaacgc caaacattcc cgattctatt ctataggtcg ggttatttga cgtacaggta
     6001 ctgttctatg gggcgcttac cttctacaag cagagctaac tgctttttta gattacgaaa
     6061 aatatgatcg aaatggattc aattcaggaa attcccgtaa cgggaattat tcacgtacat
     6121 ttaaaacaga atatggcgag ttgaatttaa ctattcctag agatcggaat ggcgaatttt
     6181 ctcaacaaac actaccagca tataagcgaa ccaatgattc attagaaacg accattatcc
     6241 aattatttca aaaaggcatt actatggctg aaatctccaa attaattgaa aaaatgtatg
     6301 gtcaccacta tacgccacaa acaatctcca acatgagtaa attggtggct gaagatgttt
     6361 tagcttttaa agaaagaacg ttagaagcca attattccgt tatatttatg gatgcgacgc
     6421 atatccctgt gaagcgacag actgtttcga aggaagcagt gtatattacg attggtattc
     6481 gtttggatgg aaccaaagaa gttcttgggt ttactattgc cccaactgag tctgcttata
     6541 tttggaaaga agttcttcaa gatcttagaa aacgtgggtt agaagaagtt ttattagtag
     6601 tgacagacgg attaagctgt attgaagaaa gtatccatag tgtgtatccg aatgcccaat
     6661 ttcaacaatg ttgtgtgcat gtatctagaa atatcgctca taaagttcgt gttcgaggtc
     6721 gaaaagaaat ttgtgaggat ttcaaattgg tttaccaagc gaattcaaaa gaagaggcat
     6781 tggatcacat cgactttatg actaggaaat ggaaaaagca gtatccaaga gtcgtcaatt
     6841 tactcttgaa tcctgcccta ttaacctttt ataatttccc tcacgccatc agacgaacaa
     6901 tttattcgac gaacctgatt gaaggcttta ataagcagct aaaacgatat actcgaagaa
     6961 aagaacaatt ccctaatgaa gaatctctag agagattctt catttctcaa tttaatcaat
     7021 ataaccaaaa atttttaggt agaattcaca agggatttaa agaaattcag gatacattag
     7081 agtcgatgat ttaactgtaa ttaacagaat ggattttcca tttacatata attcttgacg
     7141 ctaccataat ctaaataacc atcatattca tctactactt atataattga gttttattaa
     7201 actttttaag tttctattct atcaaatatt taaacaatct ctgcatccga aaagactacc
     7261 tcgtatagtt ttactaacaa tatttttatt agcgtgttca aatcctgatt tcttgtatca
     7321 attattttct gttcactttc aataagaaaa aagtctttac ttccatggat aataat
//
LOCUS       DACDYI010000103         7228 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.104, whole genome shotgun sequence.
ACCESSION   DACDYI010000103 DACDYI010000000
VERSION     DACDYI010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.104"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..1581
                     /locus_tag="IWM52_002670"
     CDS             <1..1581
                     /locus_tag="IWM52_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9974853.1"
                     /translation="IYQLQEKIRNFQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQE
                     AFEAYLKEKGVQLGKQPGTILQVETEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLE
                     AMQGLYEGKYLSYPRTDTPYITEGEYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVN
                     NKKVQEHYAIIPTKTVMTAAAFEQLSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQ
                     VQQLQLKAIEKVPLDLGWKKLFGKESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETK
                     PPQPYTEGTLITAMKTAGKTVDSEEAQSILKEVEGIGTEATRANIIETLKQKEYIKVE
                     KNKLVVTNKGILLCQAVEKEPLLTSAEMTAKWESYLLKIGERKGTQTTFLANIQKFVS
                     HLLEVVPGQIQSTDFGSTLQEVKAASEKQEAARHLGICPKCQEQEVLLYQKVAACTSE
                     ACDFKCWTTIAKKKLTATQLKEIIQNGRTSQPVKGLKGQKGSFEATIVLKEDFTTGFE
                     FSEKKKTNFKKRTRRTTK"
     gene            1822..2415
                     /locus_tag="IWM52_002671"
     CDS             1822..2415
                     /locus_tag="IWM52_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAP9974854.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMGDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            2428..3327
                     /locus_tag="IWM52_002672"
     CDS             2428..3327
                     /locus_tag="IWM52_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP9974855.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            3330..3827
                     /gene="ssb"
                     /locus_tag="IWM52_002673"
     CDS             3330..3827
                     /gene="ssb"
                     /locus_tag="IWM52_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9974856.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            4193..4453
                     /locus_tag="IWM52_002674"
     CDS             4193..4453
                     /locus_tag="IWM52_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974857.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            4898..5104
                     /locus_tag="IWM52_002675"
     CDS             4898..5104
                     /locus_tag="IWM52_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP9974858.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            5152..5355
                     /locus_tag="IWM52_002676"
     CDS             5152..5355
                     /locus_tag="IWM52_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974859.1"
                     /translation="MVDKEVISEKVAEALLIPRYWTIETPLVEDAPTMLFEDLVKIAG
                     YELHYQENMETYENEYWLTKLEN"
     gene            5371..5808
                     /locus_tag="IWM52_002677"
     CDS             5371..5808
                     /locus_tag="IWM52_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974860.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            5801..6508
                     /locus_tag="IWM52_002678"
     CDS             5801..6508
                     /locus_tag="IWM52_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974861.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            6888..7067
                     /locus_tag="IWM52_002679"
     CDS             6888..7067
                     /locus_tag="IWM52_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974862.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
BASE COUNT         2663 a         1210 c         1481 g         1874 t
ORIGIN      
        1 atttatcaat tgcaggaaaa gattagaaac ttccaaaaag aaccatattt tgaaggaaaa
       61 gcccaagtaa tcgctcaaaa tggcgcattt gatgcgaagc ttgatccaaa tgaaacccaa
      121 gcgacacaag aagcatttga agcctattta aaagaaaaag gtgttcaatt aggaaaacag
      181 ccaggtacga tccttcaagt cgaaacagag aaaaaaagtg cggctagccc acgtcttttt
      241 tctttatcga gccttcagtc taaaatgaat caactcatga aagctagtgc caaagatact
      301 ttggaagcca tgcaagggtt gtatgaagga aaatatctaa gctatccgcg aacggatacc
      361 ccttatatta cggaagggga atatgcgtat ctgttggatc acttagacga atacaagcat
      421 tttttaaaag ccgaagcgat tccgactcct attcatacac cgaatagtcg gtatgtgaat
      481 aataagaaag tccaggaaca ttacgcgatc attcctacaa aaacggttat gacagcagcg
      541 gcttttgaac aactaagtcc actgcagcag gctatttatg aacaagtgtt aaaaacaacg
      601 gtggccatgt ttgcggaaaa gtatacctat gaagaaacaa cgatcttgac gcaagttcag
      661 cagcttcagt taaaggcaat cgaaaaggtg ccgctagatc taggttggaa gaagttattt
      721 ggaaaagaga gcgacggaaa agagaaggaa gaagagccgc tacttcctaa agtgacaaaa
      781 ggagaaatgg tgacggtgga tctacaagtt cttgaaaaag aaaccaagcc accacaacct
      841 tataccgaag gtacgttgat cactgcaatg aaaacagctg ggaaaaccgt ggatagtgaa
      901 gaagcgcaat ccatattgaa agaagtggaa ggaatcggta cagaagcgac acgggcgaat
      961 attattgaaa ccttgaaaca aaaggaatat atcaaggtag agaaaaataa gctcgtggtg
     1021 acgaataaag ggattttgct ttgccaagcc gtggaaaaag agccgctgct tacgagcgcc
     1081 gaaatgaccg ccaaatggga aagctacttg ttaaagatcg gtgaacgaaa aggaacacaa
     1141 accacttttc tagccaatat ccaaaaattt gtctctcact tattggaagt cgtaccaggg
     1201 caaatccaat ccactgattt tggatcgacg ttacaagaag tgaaagcagc cagtgaaaag
     1261 caagaagccg cgcgtcattt aggaatttgt ccaaaatgcc aggaacaaga agtcttactc
     1321 tatcaaaagg tggcagcttg tacgtctgaa gcctgtgatt ttaaatgttg gacaacgatt
     1381 gcgaaaaaaa aactcacagc gacacagctg aaggagatca ttcaaaatgg ccgaacaagc
     1441 caaccagtga aagggttaaa aggtcagaag ggttcgtttg aagcaacgat cgttttaaaa
     1501 gaagacttca ctacaggctt tgaattttct gaaaagaaaa aaacgaactt caagaaaaga
     1561 acacgacgca ctacaaaata atggagggtt aatcttatgc gtaatacaac acacaaacaa
     1621 caagaaatcg aaaatcaaaa agccaaaatt cgttcagaac aggaaaaaat gcgtgccagc
     1681 aaacgaagac tgcagcaatt aaagaaccag cagcggattc aaaaagaaat tgaattagat
     1741 cgatagacat taaccttgtt acaaatacac tatcatgata tatttaatgt agtgtatttg
     1801 taacgaaagg aggggaagta aatgcaagat aaactgaaag agttgttggc tattcgcgat
     1861 ggaaatttat ctatgaagga agccagaaaa tttggaatcg cagcaaccac tgtacaaaga
     1921 ttggtgaatc aaggaaaatt gatcaaagtt gatcgtggtt actatgtaga ggatggtcat
     1981 ggaatagatg atttattttg gcttcagcaa cgattttcca ggggaatctt ctctcatgaa
     2041 acgactttag atatttacca gctttctacg aatatgccga aattcattca cttaactttt
     2101 ccccatggat ataacgttga tcgttcaatt acaaaagagc agcagttaca atttcatttc
     2161 gtgaagaaag aagtttacga gctaggaaaa gtggagggaa cttcctttca aggaaatcct
     2221 atcactatgt atgataaaga gcgtacactt tgtgatattt ggaatccgag atatgaaatt
     2281 gaatacgaaa tgaaattaga agcattaaag gagtacatgg gagatccatt gagagatcca
     2341 agcaaattaa gagattatat gacaaaacta catgtagaaa aagaaatgaa ataccatatg
     2401 caaagcttat attgatggag gaaaaagatg acaccgacac aatttgcagc aaaaacaaga
     2461 aatttagcaa agaggacagg attaaatgca cagttagtac aacggcattt tatgatggat
     2521 cgtttaattg aacaaatttc tgaatctaag tataaaaatg atttcatttt gaaaggtggc
     2581 tttttattag gatcaaaata tggaatcgat agaaggacga caattgatat cgacacgaca
     2641 tttagaaata gcaaattaac tgaagaaaaa ttgaagagta tttttaatga tttaactagg
     2701 aaacccacga aagaaggtat tgttttctct attcaaggaa tgaaggaaac aagagaagcc
     2761 gatttttatc ctggatttca aattaaattg gtggctaatt tagagaaagt acgtgtgcca
     2821 tttaaaatgg acgtgacaac aggagattct atttatcctg aagtagatac acactatcac
     2881 caattaatgt ttgaagataa aaaagtggag attccagctt atccaacagc acaaattatt
     2941 gcagaaaagc taagtgctac gttcagcttt ggcacagata attcgagagc aaaagattac
     3001 tacgacttat ttaccattcc gaaactagaa aatatcgata cgaaagagtt atacaaaagt
     3061 gttcgaaata catttacaaa acgtggggat acaaaaccgt tgtcctttta ttatgaaaga
     3121 gaaatggata agatcagagg aaatgactat ttggaaaagc agtgggaaaa atatcgctca
     3181 gttaacacgt ttgcgaattc catttcattt acagatacag tcaacgaaat tgatcaccta
     3241 atgaaaagtg tgatcaaaat tgaaaatcaa gaaagaaaac ggcagtttaa aagatcacaa
     3301 caaatgaatc aagaaatgga gagataaaga tgatcaataa tgtaacttta gtaggacgct
     3361 taaccaaaga tccagattta cgttataccg cgagtggtac tgcagtagcg acttttaccc
     3421 tagctgtcaa ccgaaatttc acgaatcaaa acggcaatcg agaagctgat tttattaact
     3481 gtgtgatttg gcgcaaacca gcagaaacaa tggcgacatt agctaaaaaa ggtagcttga
     3541 ttggtgtagt tgggcggatt caaacgcgga cttatgacaa ccagcaagga caacgtgtgt
     3601 atgtgacaga agttgtggcc gataatttcc aactattaga aagcaaagca gcgacagaaa
     3661 gccgtgcgca cgctgatcaa tcgtccacca gtccaagcac caccactttt gaacaacggg
     3721 atacagcgac gcctaataac aatggattga acgctttaca gaatccattt gggaatccat
     3781 ttggtggaca atcaatcgat attagcgatg atgatctacc gttctaagta gcattttcta
     3841 ttggttgcat ttgctacggt ttatgttata atttagtcaa caaaaagaat tttcaatcta
     3901 taaccaaaaa ccactagctg gcgggctagt ggttttcttt tacgtttcta ttataagaat
     3961 taacgggtag gcttactgtc tttcttggca aatttacaat caagccattt gcagatatag
     4021 tgcgcggcta cttctgccat gattgcaaaa acaaaatcaa aaagaatttt caaacctata
     4081 acctcctccc cttgccagat taggccgggg tagcagacag caagactatt atagcataat
     4141 tgaaatatcc aaatgattgg atttttgcta taattttagc gaggtgattg tgatgaacaa
     4201 atatgttggt ttgcttgata aaatccgagt aataaaaacg caaccgttat tggttcgctt
     4261 tactttacaa acgattcatg aatcgattaa ttgtgtagtt gcagatattg aaattatcga
     4321 taaattgcta attatggatg atggaaaata caatattgcc gtcacaggtc acttcaataa
     4381 acgaaatcag ctagttattg ctagcatgta tgttagaaat cccgatcatt ttacgcgatc
     4441 gatgggcata taaaaaaaga agagttgcaa tcctaatgta ggactgcagc tctttttgtt
     4501 ttacatagaa acaagaaaaa tgaaaaggcc tttttaggaa ggcgcgagcg ccttctcaat
     4561 tgaaaaacct ctctcactcc aaccgccacc gccgttcgag aagaaaggaa gtgaacgttg
     4621 cacgttcact aagataaagg gtcttgcaaa gcaagacaaa atagaaaaaa cttcctctca
     4681 ccaaaaggtt cgttcctgtt cgttatctct ttattgattt gccagaccgg caacaagttg
     4741 ccttttatca ccaaaaagcg acaaggacaa gggtaaatgg cacgctgcgc gcccttgtcc
     4801 ttgtcgcttt tcgttgattc attctggcca acaaatcaat aaagaaagaa ctaacgaaag
     4861 gaagtaaaac ctttcctaag aggaggcaga atcaatcatg cgaaagaaat ttgattattg
     4921 gggtgttccc ttcagcgaat tattcccaaa ctatcatgca ccacatactg tcgaatgcga
     4981 ttgtggacaa agagcgaaat gtatcaaaag ctatcgtttg tatcaatgtc caacatgcgg
     5041 aaaaaaatac accttgtctt atggcgatta tattttgatt gatgagaaag gaaagaaacg
     5101 ctgatgaaaa cataacataa attattgact gtaaaagaag tcgttcaaat tttggtggac
     5161 aaagaagtca tttctgaaaa agtagcagaa gctttactta ttccgcggta ctggacgata
     5221 gaaacccctt tagtagagga tgcaccgact atgctatttg aagacctagt gaagattgca
     5281 ggatatgaac tgcattatca agaaaacatg gaaacttatg aaaacgaata ttggttaacc
     5341 aaattagaga actaaaaagg agaaaaaaat atggctcaaa attactatga cgaatttgtc
     5401 aaattacctt tggataaaat ggcacaaaaa atggaggata tgacattcct ataccatgag
     5461 acacgtgttc caaaaaaaca ttataaggaa agattgtcgg tagctgtgga agaaatgatt
     5521 gaatcaggtg tcgaaatgaa tctcattgcg acgtactatc gaacactgga agaactgaaa
     5581 aaacaaaatg caaaatggtt tttccaagcc ttactatgct tggaagtagg tgtcaaacca
     5641 agcactatta aaccttctga atatcaagct ttagaactaa cgtatgggaa atttgtcgaa
     5701 acgaaaaaag ccaagactgt ttcaagtgaa tggttagatt attttgaaaa tatcaataag
     5761 tatggcgcct attacaccat gaaaaaggag gacaacgaaa atgaataagg tgatttatca
     5821 actaacaacg gaagatgtgt cacacttttt aacaaacaaa gaacatttga atgaagtaat
     5881 cagacggatt caaaaagact ttagttgtat ggaagaaatg aagtacttta ttcaaagagt
     5941 cgaagaacaa ttgtatttgg aaagtgaaac gagtcaaacg aaggtcacag tcagcaataa
     6001 ttatttagac gtgtataaac atcgtcctga aacggtaaaa aaaggaacgg agcttctacg
     6061 actagaaaca gaaaaataca tcatgaatgt ttacctagaa aggagtgaac tggtcgaaaa
     6121 ctttgtcagc tgcacgattt gcaggaaaca ctattactca gaatcagaat attattcaat
     6181 ttttgagagc atggaattaa tggaagaaga aatggaagag ctaaacagca aaaatgcgac
     6241 gatagacgat tattatcttg tatttactag atttttgacg tgtggaacgg aatctatcgt
     6301 gttaatggga atggattatg aactcgctgt acccgaagca tttttgaggg aatatgtaga
     6361 agaagtagat aacccaggcg acgactggta cacgacttta actgattttt tagataccta
     6421 cacttacgac gatacagaag aaatccaaaa tgaacttgac cgtaaacgaa tgccatatca
     6481 acgatttgaa aggaaaaaaa ggcaataatc atttataccg aaaagtgaga atgtgtgaaa
     6541 taagaacaaa aaccttgaaa ttaagggaat ggttcttatt tcgcaccaat cgctccaacc
     6601 gcccccgccg cttaggtggt gcgaaataag cgggcaaagc ccgataacct aagagaaaga
     6661 aggagatcca tatgaataaa atcattcctc tgttgttcgt actgtgtatt ggcgagatac
     6721 ttatgcgtca tggtgcagct gtacatttaa acttcgtagt attcttagga atactagtat
     6781 tttgtttgat tttttggcgc tttgtgttag cccttgtgag tctactattc aagttgattg
     6841 cagctatatt tttcatagga ttatttcgat gaaaggagag agtaccaatg ccaattgcaa
     6901 taggaaataa acggttaccc gtgacattag atgaaaagag acaaaaagaa ttgcagcaac
     6961 taaagcagaa gtacggaaaa agtgaatcta agattatgtg tattgcgtta gatctattga
     7021 tcgcgcaaga aaaagcagga tttgatatac cagcactaaa aaagtgacgt cacttttcat
     7081 ttgaagaaac aaaagtgata gcacttaatt gttcctacta tttagaatat tttctatgtc
     7141 ctttttattc cattttgtat caataactat gaaatcttta aagtatccag aagagattat
     7201 ttttagatca atttttttct tacctgat
//
LOCUS       DACDYI010000104         6667 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.105, whole genome shotgun sequence.
ACCESSION   DACDYI010000104 DACDYI010000000
VERSION     DACDYI010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.105"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            356..952
                     /locus_tag="IWM52_002680"
     CDS             356..952
                     /locus_tag="IWM52_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9974863.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPERTLCDIWNPWYTVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            965..1864
                     /locus_tag="IWM52_002681"
     CDS             965..1864
                     /locus_tag="IWM52_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP9974864.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKDGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNEIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            1867..1998
                     /gene="ssb"
                     /locus_tag="IWM52_002682"
     CDS             1867..1998
                     /gene="ssb"
                     /locus_tag="IWM52_002682"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9974865.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGN"
     gene            2020..2352
                     /gene="ssb"
                     /locus_tag="IWM52_002683"
     CDS             2020..2352
                     /gene="ssb"
                     /locus_tag="IWM52_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9974866.1"
                     /translation="MIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEV
                     VADNFQLLESKAATESRAHADQSSTSPSTATFEQRNTATPNNNGLNASQNPFGGQSID
                     ISDDDLPF"
     gene            2374..2631
                     /locus_tag="IWM52_002684"
     CDS             2374..2631
                     /locus_tag="IWM52_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974867.1"
                     /translation="MAKKISLTMDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVF
                     VYLVCSYLLKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(2790..2912)
                     /locus_tag="IWM52_002685"
     CDS             complement(2790..2912)
                     /locus_tag="IWM52_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAP9974868.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            3102..3326
                     /locus_tag="IWM52_002686"
     CDS             3102..3326
                     /locus_tag="IWM52_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974869.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRKQLVIESMQIRNPDHFTRSMSI"
     gene            3769..3975
                     /locus_tag="IWM52_002687"
     CDS             3769..3975
                     /locus_tag="IWM52_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP9974870.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            3975..4226
                     /locus_tag="IWM52_002688"
     CDS             3975..4226
                     /locus_tag="IWM52_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974871.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEVLLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            4407..4679
                     /locus_tag="IWM52_002689"
     CDS             4407..4679
                     /locus_tag="IWM52_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974872.1"
                     /translation="MNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQ
                     ALELTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            5124..5303
                     /locus_tag="IWM52_002690"
     CDS             5124..5303
                     /locus_tag="IWM52_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974873.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            5362..5718
                     /locus_tag="IWM52_002691"
     CDS             5362..5718
                     /locus_tag="IWM52_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974874.1"
                     /translation="MLKTKKNSYKLKLSILSIICLLGMIISGHLYRVAYTQFIEYKVH
                     SLGLAGDRYVEYVHYSLKCSSYILLWLIFSLFELLNLIAISSYHLDKLNTKSSRYNEV
                     FKENVKLESDLNKLKK"
BASE COUNT         2399 a         1064 c         1316 g         1888 t
ORIGIN      
        1 gttcatttga agcaacgatt gttttaaaag aagacttcac tacaggcttt gaattttctg
       61 aaaagaaaaa aacgaacttc aagaaaagaa cacgacgcac tacaaaataa tggagggtta
      121 atcttatgcg taatacaaca cacaaacaac aagaaatcga aaatcaaaaa gccaaaattc
      181 gttcagaaca ggaaaaaatg cgtgccagca aacgaagact gcagcaattg aagaaccagc
      241 agaggattca aaaagaaatc gaattagatc gataaaatct tcctaactat tgtatacgta
      301 taaacttatg ttatactgtt tttataagtt tatacgtata caaaggaggg agaaaatgac
      361 aaaagcaatg gtatacaagc tgctagaaga atttaatgga actttggctt tgaaggatgc
      421 caaaaaagcg ggcatttctc ctgtaacgat caaacgtatg gtagatcgag gggagctgga
      481 tcgagagtat ccaggttttt tcaccttacc tggtcaattt cctgatgaat tgtttatggc
      541 gcaaaaaaaa tatgaacgcg gcattatttc acatattacc gcgttagatt tgtatgattt
      601 aacagatatg atccctagac agatcgatct gactgtacct tacggttatc acgtttctga
      661 aaaaggattg aaggaattcg cagttgagct acactataca aaagcggaat ggtatgaatt
      721 aggtaagatt gaaataaaaa gtcgctatgg gaatcctatt atagcttatg atccagaaag
      781 aactttatgt gacatttgga atccctggta taccgttgag gatgaaatca aagtaaaagc
      841 aatcaaaaac tatatggagt cggacagaaa aaatcttaga aaattgaatg agtatcgacg
      901 aattttacca acagacaaaa cgatgcgttc ttatattatg gctttaaatt aaaggagaag
      961 atacatgaca cctacgcagt taaaagcaaa agtgaagaat atttctagag aaaaagaagt
     1021 tgatccgcag cttgttttaa gacattttat gatggaaaaa ttcttggaaa aaatctcaga
     1081 gtctccttat cgtgaaaatt ttgtgttaaa agacggcttc ttgattggct caaagtatgg
     1141 cattgaaaat cgcacaacca aagatattga tacgacttta cgggaaatga aagtaaccaa
     1201 agaaacatta acaacggttc taaacgaaat tttttcaacg ccgacgaaag aaggcattca
     1261 atttgagatt caaggaatga aagaaacgag agaagccgat tactatccag gatttagctt
     1321 acgtgtgctg gcacatttgg aaaacatgcg gccagatttt aaggtagatg tgactacagg
     1381 tgactcgatc tatcctgcaa cgatcactca cagtcataaa ttgatgtttg aggatcgaac
     1441 gattcctttg gaatcctatc ctacagaaca aattattgct gaaaaattaa gtgcaacctt
     1501 tgatttttta acagataatt cgagaggaaa agatttttat gatctctata ctattcctaa
     1561 aatggagaag atcgatgaga agaccttgaa agatagtgtg agaaatacat ttacacgtcg
     1621 aggaaaaact gatccgttaa agatatacta tgaaaaggat atggatgtac tgaaaaatag
     1681 tcacgagcta aaagatacct ggaagaaata tcaaaaaagc aatccttatg ctgcgtcgat
     1741 cacgtacgat gagacaatgg attcgattag taatttgatg gaaaaagtga ttcacgtcga
     1801 agaaaaagag cgaaaactag cttatcaaag acaccaacag atgaatagag aaatggagag
     1861 ataaagatga ttaataatgt gacattagta ggacgcttaa ccaaagatcc tgatttacgt
     1921 tataccgcaa gtggtactgc agtagcgact tttaccctag ccgtcaaccg aaatttcacg
     1981 aatcaaaacg gcaattgaga agccgatttt attaactgcg tgatttggcg caaaccagca
     2041 gaaacaatgg cgacattagc taaaaaaggt agcttgattg gtgtagttgg gcggattcaa
     2101 acgcggactt acgacaacca gcaaggacaa cgtgtatatg tgactgaagt tgtagcagat
     2161 aactttcaac tattagaaag taaagcagcg acagagagcc gtgcgcacgc tgatcaatcg
     2221 tccaccagtc caagcaccgc cacctttgaa caacggaata cagcgacgcc taacaacaat
     2281 ggattgaacg cttcacagaa tccatttggt ggacaatcaa tcgatattag cgatgatgat
     2341 ctaccgtttt agtttgagaa agaaggagta agcatggcaa aaaaaattag tctgacgatg
     2401 gatctattga tacccgtaat tgcatttata gacgggattt ctttgggcgt cactgaaaat
     2461 cctttggtaa atagaattgc gaaaggatca ctatttcttg tattcgtgta tcttgtctgt
     2521 tcttatcttt taaaaagagc aggaaaggat aattcctttt ggcgattgac taaagtcaca
     2581 gtagcagcga cagctgttat acttacggct attctaccgc tttatatgta gagttaacgg
     2641 gaaatcgttg aacttcttgc gaaatattgc tataatgaac atgaaaaaga ggagttgcgc
     2701 taacaacccc tcattaacag caccgattaa agaacggtgg cagaatatta gttatttgga
     2761 aataaccgct gacctgctaa agtagacggt tatttctttt tgtcattttt aagcatattt
     2821 acaatcaaac taatcaaagc aatggtaaac ataccaaaac caagaatggt ttgaattgtc
     2881 tcatatgcgg acaaaaggct tactcctttc atagatttta gcacttacat tcataagcac
     2941 caccaccttt ccagaagtcg ccaccgtctt caaactttac tgtcttgact attatagcat
     3001 atctaaaatc gaataaaaat tctcaatttt agctgttttt gctataattt tagcgaggtg
     3061 atcgtgatgg aacaatatat tggtttactc gataaattag ggtgatcaag acacagccat
     3121 tgcttgtgcg atttacatta cgcacgagtc atgaaccaat caattgtgta gtggccaata
     3181 tagaaatagc ggataaattg ctgattttgg aagaaggcaa gtatactatt tcagtgacag
     3241 gtcacttcaa taaacgaaag caactagtca ttgaatcaat gcaaattaga aaccctgatc
     3301 attttacgcg atcgatgagc atataaaaga agagctgcag tcctaaatga ggattgtagc
     3361 tctttttgtt ttacatagaa acagtaaaaa tgaaaaggcc tgtttaggaa ggcacaagcg
     3421 ccttctcaat tgaaagacct ttctctcact ctaaccgcca ccgccgttcg agaagaaagg
     3481 aagtgaacgc tacgcgttca ctaagataaa gggtcttgca tagcaagaca aaatagaaaa
     3541 aatttcctct caccaaaagg ttcgctcctg ttcgttatct ctttattgat ttgtcagacc
     3601 ggcaacaagt tgccttttat caccaaaaag cgacaaggac aagggtaaat ggcacgctgc
     3661 gcgcccttgt cttgtcgctt ttcgttgatt cattctggcc aacaaatcaa taaagaaaga
     3721 actaacgaaa ggaagtaaaa cctttcctaa gaggaggcag aatcaatcat gcgaaagaaa
     3781 tttgattatt ggggtgttcc cttcagcgaa ttattcccaa actatcatgc accacatact
     3841 gtcgaatgcg attgtggaga aagagcgaaa tgtatcaaaa gctattgttt gtatcaatgt
     3901 ccaacatgcg ggaaaaaata taccttgtct tatggcgatt atattttaat tgatgagaaa
     3961 ggaaagaaac gctgatgaaa acacaacaca aattattgac tgtaaaagaa gtcgttcaaa
     4021 ttttggtgga caaagaagtc atttctgaaa aagtagcaga agttttactt attccgcggt
     4081 actggacgat agaaacccct ttagtagagg atgcaccgac tatgctattt gaagacctag
     4141 tgaagattgc aggatatgaa ctgcattatc aagaaaactt ggaaacttat gaaaacgaat
     4201 attggttaac caaattagag aactaaaaag gagaaaaaaa tatggctcaa aattactatg
     4261 acgaatttgt caaattacct ttggataaaa tggcacaaaa aatggaggat atgacgttcc
     4321 tatacaacga aacacgtgtt ccaaaaaaac attataaaga aaaattgtcg gtagctgtgg
     4381 aagaaatgat tgaataaggt gtcgaaatga atcttattgc gacgtactat cgaacactgg
     4441 aagaactgaa aaagcaaaat gcaaaatggt ttttccaagc cttactatgc ttggaagtag
     4501 gtgtcaaacc aagcactatt aaaccttctg aataccaagc tttagaacta acgtatgcga
     4561 aatttatcga aacgaaaaaa gccaagactg tttcaagtga atggttagat tattttgaaa
     4621 atatcaataa gtatggcgca tattacacca tgaaaaagga agacaacgaa aatgaataag
     4681 gtgatttatc aactaacaac ggattccata tcaacgattt gaaagaaaaa aaggtcaata
     4741 atcatttata ccgaaaagtg agaatgtgtg aaacaaggac aaaaaaagcc tagtaactaa
     4801 ggaaatggtt cttatttcgc accaatcgct ccaaccgccc ccgccgctta ggtggtgcga
     4861 aataaacggg caaagcccga taacttaaga gaaagaaggt gatccatttg aacaagatta
     4921 ttcccttatt gttagtactg tttgttggcg aaatacttat gcgagatagt gcagctgtac
     4981 agttaaacct cgctgtattt ttggtaatac tggtgttttg tttgatcttt tggcggtttg
     5041 tattagtcct cgtgagtcta ctattcaaga tgattgcagc catatttttc atagggttat
     5101 ttcgatgaaa ggagtgagta ccaatgccaa ttgcaatagg aaataaacgg ttacccgtga
     5161 cattagatga aaagagacaa aaagaattgc agcaactaaa gcagaagtat ggcaaaagtg
     5221 aatctaagat tatgtgtatt gcgttagatc tattgatcgc gcaagaaaaa gcaggatttg
     5281 atgtaccagc gctaaaaaag tgacgtcact ttttaatttg atctatataa gtgatatcac
     5341 ttttaccaag gagggaatta aatgttaaaa actaaaaaga attcgtacaa attaaagctt
     5401 agtatactat ctattatttg tcttttaggc atgataatta gtggtcattt gtatagagtg
     5461 gcatatacgc agtttattga gtacaaggtt cactctttag gattagctgg ggatcgatac
     5521 gttgaatatg tccattattc attaaaatgt tcttcatata ttcttctttg gcttattttt
     5581 tcattgtttg aattgctaaa tttaatagca atatcttcat atcacttaga taagctaaat
     5641 accaaatcta gccgttataa cgaagtgttt aaggaaaatg tgaagttaga aagtgactta
     5701 aacaagctaa aaaaataaaa agaagaaaac ataagttaac taaaattatt ttattaagaa
     5761 tgatggaata taaggataag gttaccaaca taacgatcat tacttctctg atatgctatg
     5821 gacttctatc aatcagtatt ttgtctatag attatggagt aactatactg caagccttag
     5881 ttttggctat gcctatgtat ctatctttgt ttatcatgca tgaaatatat aagctatttg
     5941 ttttatcaaa aaatgtagaa atacgggtaa atattttggt gaatagttat caaaaagaac
     6001 tcaaagaaag aagacggtgg cggtatttat tgaaaggatg aaaaagtagg atttgacata
     6061 ctggtattca gaaagtgccg tcacttttaa aacaatggaa aaggtggaga taagatgaag
     6121 aaaaaacaaa ctaaactatc attagcagat atttaacaca gttaaccgca actgaagatg
     6181 gtgttgttga atttgaacaa tctgaaattg cggtagtaga tgatcgttac cttaaaatgc
     6241 cttacttctt tgaccaagca aaggtcattt gcctttgtca ctacgatggg gtacgtgatt
     6301 ttaggcgtta aaaagtagtg attatatcaa ctttacgagt aaatataaat ttagacatga
     6361 aactgaatta tccaatggaa aatcatggtt tataatctag aaaaattata gtaatgattg
     6421 gattatatat attctttcgt attttcgtac aactgtagct tgtcaaaatt gagaaattta
     6481 accgattagg tactttacat cgttatttgg gtaatttcta aaacatgaag cttaccaacg
     6541 aataaactga gactatctta ttaattgatc aatataagaa aatatctttg aggagatgat
     6601 gtactatgtt ttcgtctatc cacgattggt taggtggtct tttcggcggt ggcggtaaag
     6661 gttttta
//
LOCUS       DACDYI010000105         6643 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.106, whole genome shotgun sequence.
ACCESSION   DACDYI010000105 DACDYI010000000
VERSION     DACDYI010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.106"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(1..859)
                     /locus_tag="IWM52_002692"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="IWM52_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="IWM52_002693"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="IWM52_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAP9974875.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="IWM52_002694"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="IWM52_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="IWM52_002695"
     CDS             complement(2143..2373)
                     /locus_tag="IWM52_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAP9974876.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="IWM52_002696"
     CDS             complement(2603..3421)
                     /locus_tag="IWM52_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9974877.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="IWM52_002697"
     CDS             3582..4271
                     /locus_tag="IWM52_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP9974878.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="IWM52_002698"
     CDS             4285..5787
                     /locus_tag="IWM52_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9974879.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="IWM52_002699"
     CDS             5800..6276
                     /locus_tag="IWM52_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP9974880.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="IWM52_002700"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="IWM52_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1031 c         1204 g         2236 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gttttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atg
//
LOCUS       DACDYI010000106         6136 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.107, whole genome shotgun sequence.
ACCESSION   DACDYI010000106 DACDYI010000000
VERSION     DACDYI010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.107"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(207..1169)
                     /locus_tag="IWM52_002701"
     CDS             complement(207..1169)
                     /locus_tag="IWM52_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9974881.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="IWM52_002702"
     CDS             complement(1171..2610)
                     /locus_tag="IWM52_002702"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAP9974882.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="IWM52_002703"
     CDS             2795..4741
                     /locus_tag="IWM52_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAP9974883.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            5005..5877
                     /locus_tag="IWM52_002704"
     CDS             5005..5877
                     /locus_tag="IWM52_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9974884.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT         1946 a         1054 c         1085 g         2051 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaggc acatttaaaa ttgatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaatatttg agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta
     4861 ttagaatttt aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca
     4921 tgttactaaa taaaaaaata atttaaatat gatatatagt catttattta gtgatttcat
     4981 tctaaaataa agggagaaat gtctatgcta tacggtgcaa ttgaagcagg cggcacaaaa
     5041 tttgtgtgtg ccattggcga tgaagaaatg acgatcaagg aaagagtgag ctttccgaca
     5101 acgacacctg aggaaacaat gcctttagtc attgacttct tcaaacaata ccaagcagat
     5161 ttagcaggga ttgggattgg ctcatttggg cctatcgata tccatcgcga ttccgccact
     5221 tatgggtaca ttacctcgac cccgaaattg gcatggcaaa attttgattt catcggtacc
     5281 atgaaaaaag aatttccgat cccgatttct tggacaacag atgtcaatgc ggctgcctat
     5341 ggtgagtatg tcttcggcag tggcaaagga ttgagcagtg tcgtttacta tacgatcggt
     5401 acgggtgttg gcggcggtgc cttacaagaa gggcgcttta tcgaagggtt tagtcatcca
     5461 gaaatggggc atatgctcgt cgtccctcat ccaaaagatg actttgctgg cagctgtcca
     5521 tttcatggca actgtttaga agggatggcc gctggacccg ccatcgaaaa acgcttaggg
     5581 aaaaaaggac aagaggttgc agaagatgat ccttattggg aaatcgaagc ctcctatatt
     5641 gctcaatgtg cctacaatac aaccttgatg ttttctccag atgtgatcat ttttggcggt
     5701 ggtgtgatga aacaacgcca tatgctgcaa aacgtccacg acgcatttgc aaaattggtc
     5761 aatgggtatg tcgaaacacc tgagttaagc aaatatatcg tgaccccagc attagaagac
     5821 aacgctggaa cgctcggttg tttggcattg gctcgagaag cggtcttaca ttcataaacg
     5881 aaaaaaggct cttcgacatt tagtgttggt agagaaagat acaactgaaa atttagtcat
     5941 aaccagttag gcagagtgct taactggttt ttgttttact tggaagatca agccttgaat
     6001 gagataaatt cttgctcgaa agttatggaa atttcgatag ccgtaggcca cttgtattgt
     6061 gcaatagttt tttacaaaaa aaagggggtg cggatatttt tttggacctt tttaacaaat
     6121 atatctttgc ttcttc
//
LOCUS       DACDYI010000107         6124 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.108, whole genome shotgun sequence.
ACCESSION   DACDYI010000107 DACDYI010000000
VERSION     DACDYI010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.108"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..189
                     /locus_tag="IWM52_002705"
     CDS             <1..189
                     /locus_tag="IWM52_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9974885.1"
                     /translation="NSNGILRRNGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
     gene            complement(275..1369)
                     /locus_tag="IWM52_002706"
     CDS             complement(275..1369)
                     /locus_tag="IWM52_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP9974886.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1558..2319
                     /locus_tag="IWM52_002707"
     CDS             1558..2319
                     /locus_tag="IWM52_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9974887.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2434..2874)
                     /gene="fabZ"
                     /locus_tag="IWM52_002708"
     CDS             complement(2434..2874)
                     /gene="fabZ"
                     /locus_tag="IWM52_002708"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP9974888.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            3094..3849
                     /gene="fabI"
                     /locus_tag="IWM52_002709"
     CDS             3094..3849
                     /gene="fabI"
                     /locus_tag="IWM52_002709"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAP9974889.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3959..4972)
                     /locus_tag="IWM52_002710"
     CDS             complement(3959..4972)
                     /locus_tag="IWM52_002710"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAP9974890.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            5137..6057
                     /locus_tag="IWM52_002711"
     CDS             5137..6057
                     /locus_tag="IWM52_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAP9974891.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1880 a         1214 c         1094 g         1936 t
ORIGIN      
        1 aattctaacg ggattctgcg tcgtaatgga ctgccgaaat caatggattt tagaaaagtg
       61 aatcagacat ttatttccag tgtcagcaat caacgtaatc atattccaag aaaatcattg
      121 aattacagaa caccaatcga aatatttttg agctatgtac aagaagcatt ttattctagc
      181 ttaatttgac aaatcataac ctataaaaaa acaaaataaa aaagcagaaa taactggcta
      241 tttctagcgg attatttctg caataactaa ttatctattt tttattttca attaatttaa
      301 agcttgacca tggtttcaaa aagtcaagga ggaataattc gtcatcggag attcttccta
      361 caacattgac acgtccgtcg tttttcatat ccttcaaggc gatttgtgtt tctcctttgt
      421 attgaccgta cccttcattg tcaatcagta catccccatg atgcatatct cgcgtattat
      481 gaggcgggaa atctttatct ttgtagtata cacgagtcat cgttgaacga aggatatatt
      541 ctgagcgatc ccctcgataa ctatgaaggt tatcaaacaa gcagattcgt tcgttttctg
      601 taataccttc ttccgtatct actttcaatg ttggataatc cgcgttaaat gcttctgcca
      661 tttctttcaa ttcttcttca cttgcgtacg catttccaat agaaatatca tcaatcaatc
      721 ctgtaaggat taaatgcttc acttgtgtcg caatttcaag atctcggtgg tcttccagcg
      781 tgcacaagcc atcttgcgta ggccaagggc caaactcagc agattgcgaa ttgacgaatg
      841 ccatcgtatt caaattatat tttctgaatt tttctgaaca aaaaacaaag tgttcgtatc
      901 ctaaaccaga gtatcgatga ggataaaaat tatgagaacc taataaatta tccgtgtttg
      961 gcgaataact catgatgttg tcaacgtaat tcgtgcccga actcatattg atctcgattt
     1021 tgataccaaa aggattgcgc gtcatttttg cttcttctgc tcctgtgaat ccgatatcta
     1081 agcggacccc gtaggcaccc atcttatgga aaaaagataa gtcatcgtaa gaaatctcca
     1141 actgttcaaa taacgctgga ttgatatcaa ccattacttc catgcctagg ctattcgcgt
     1201 aatcaactac ttctttaaat tctgaaagaa ctttttcttt atcatcattg atctgcagca
     1261 aactggtaaa aacccgttta tacccatact tatgagctaa atccaagtac gctttgtcct
     1321 tttcaaatgt tgaccgttct ggataaatcg agattcctaa ttttcccata ttaccatttc
     1381 ctcctgcatc ttttcgtttt tgtatccact tacattatag caaacacaaa gtaaatggtc
     1441 tacacctttt ctttttcttt taaaataaat gataaattcc aataatttaa gtaggtattt
     1501 tccctctatt ttgttatcat taaagataac aaaatagtaa attggaaagg aacagttatg
     1561 aagaaaaata aagttatttt aagtattagt ttagcagcag cattgctgtt tgctgctggt
     1621 tgttctgcaa acaacacgac ttcttctagt tcgtctacag aaagttcaaa aagtacatct
     1681 actgcggaaa gcacgactgc cagtagcgtc gatttgaacg cattggaatt accacaattg
     1741 acgaaagatg tcgccgaaaa tgaagatctg gtcgaaatgg tgacgaataa aggcacaatc
     1801 aaaatcaaat tatttcccga acacgcacca aaagcggttg aaaactttat gacacatgca
     1861 aaagacggtt actacaacgg actgactttc catcgcgtca tcaaagattt catgatccaa
     1921 ggtggcgatc ctaaaggcga tggcactgga ggggaaagca tttggggaga aggatttgaa
     1981 acagagatca acaaccacct ttataatata agaggtgcgt tatctatggc tcgctcacaa
     2041 gatcctaaca gtaacggcag ccagttcttt atcgttcaaa atacagatga tatgcatgat
     2101 ggtcttttaa aagacgacta tcctcaagca atcatcgacg cttataaaaa cggcggttat
     2161 ccttcactag atggccatta tactgtcttc ggccaagtga ttgaaggaat ggatgtcgtg
     2221 gatgctattg caaatatcga aacagatagt tcagacaaac caaaagaaga agtaaaaatc
     2281 gataaaatca atatactgca agaagcaaaa caaaaataaa aaaataacgt ctgaatcctg
     2341 catagatgac aacgtatgct aggattcagg cgtcttcttt ttacataaaa agcaagtaag
     2401 cttccttttg ttttgctata tcaggtttta tagtcaagca ttttggttga cgatgaacgt
     2461 gaactcgcac tcacagactt ttttatcatc tacatatgct gcagataacg ctgtaccgac
     2521 tgatcccttc attttggtga tttcaaaatg acatttcagc acatctcctg gaacaacttt
     2581 ttgacggaat ttggccttat tgatcccacc gatataagct ctttttccta gaaactcttc
     2641 tgattttagg ataagtatcg aacctgcttg tgccagtgtt tctagaatca acgcacccgg
     2701 catcactgga ttgtcaggaa agtgaccttg gaaaaaatct tcattgattg ttacattttt
     2761 tgtcgcaacg atcttttttc ctggttctaa agtatctaca taatcaatat aacaaatagg
     2821 ataacgatta ggaatcaatt ccattacttc agtagcagat aatacgtaag acataattaa
     2881 actccttctt tcaggcaatt gccattttca ttactttgac tatcaaagta tcatattcct
     2941 aaatatttag caatcttttt ttaatttaag aaaaaatagt ttgacaatca aaacattttg
     3001 actaataatt aagaaatcaa gaagttattt tagctattat tctctatttt tttacgacta
     3061 tcaaattttt atcagaaaca aaggagagtc attatgtcat ttctagaagg aaaaaaaatc
     3121 gtcattatgg gcgtcgctaa caaaaaaagc attgcttggg gttgtgctga agcaatgttg
     3181 aaacaagggg cagaaattat ctatacttac caaaatgaac gaatgaaaaa atcattagtc
     3241 aaattggtcg gcgaagaagc attcactgta gaatgtgatg tttctagcga cgaaagcatc
     3301 gaaaaagcaa tgaatgccat cggtgaatat gctggggaga tccacggcct tgtccatgca
     3361 gttgcttacg ccaacaaaga agaattgtcc ggaaatgtgt ctgatatttc cagagacggc
     3421 tatcaattag ctcaagatat cagcagctac tctttaattg ctgtatcaaa atacgcacaa
     3481 ccgtttcttg caccaaacag cgggatcgtt agcatgactt atctaggttc agaacgcgca
     3541 atcccgaatt ataacatgat gggtattgcg aaagcttctc ttgaagctgc tatccgttat
     3601 ttggcagctg agttctcgcc taaaggaatt cgggtcaatg gaatctcagc tggggcaatc
     3661 aaaacattag ctgtcacagg tgttaaagac tatcaaaaac tgatccagtt atctgaatct
     3721 cgtacgcctg atcagcaagg tgtgacgatc gaagaagtcg gtaatgcttg tgctttctta
     3781 atcagcccac tttccagcgg aatcatcggt gatattatct acgttgacaa aggtgtccat
     3841 ctttcataaa cttttgttgc attctttaaa gtaaaacata taaaaatcag gaaaaaactt
     3901 tttaaagtct cttcctgatt ttttcttttt acgttttttt caactcatta ctttgaaatt
     3961 attccacgac ttcaatccag ccttctggtg cttctatgtc tccaaattga atacctgtca
     4021 attcatcata caaccgtttt gttactggtc ctacttccgt ttcactataa aatacatgaa
     4081 gattgttatt gtgataaatc ccaccgattg gagaaataac agccgctgta ccacatgcac
     4141 cagcttctgc aaattgatct agatcatcaa tgaaaatatc tccttcaatt gcttgcagtc
     4201 ctagtcgttc ttcagctaac tgcaacaagg aatatttcgt gatacttggt aagatagagg
     4261 gcgattgcgg agtgatgaat tttccgtctt ttgtaattcc aaagaaattt gctgcaccta
     4321 cttcttcgat tttcgtatgt gtggctggat caagataaac acaatctgag taatccagtt
     4381 cttttgcttc tgttcctgga agtaaactcg ctgcgtaatt tccgccgact tttgctgcac
     4441 cagtcccctt aggagcagca cgatcgtaat cagatacaat aaaattcgtt ggttttagtc
     4501 cacctttgaa atacgctcct actggcgtac agaagacgac aaataaatat tctggagctg
     4561 gatggacacc aatgttttca ccgaccccaa tcagtaaagg gcgcaagtat aatgtagcgc
     4621 ctgttccata gggcggcaca aaatcttcat ttgcttttac cacttgcttg acagcatcaa
     4681 cgaacatctt ttcagggacc attggcatca gcaatcgtct agcactttga ttcatccgtc
     4741 gactattttg ttcaggtcga aagagattga ccttgccgtc ttttcgacga taagctttta
     4801 gaccttcaaa acattgctga ccataatgaa gcgctggaga gccttcatga atcgtcaggt
     4861 aattatcttc tgtcaattct ccttcttccc attgtccgtc tttccatttg gcaataaaac
     4921 gccaaggagt tttgatataa gaaaaaccta gattgttcca atcgatttgc atatacttac
     4981 caccttcgtt attttctcca cattttatca ttatccatat tatttgtcat tttattttca
     5041 gcttttttcc ttcattatag ttatttttgc tatttccttt tttgaaaact tcttggtaca
     5101 atacagaaag aatcaaattt tagaaaagag gattttatgt tttcattatt agcaactgcc
     5161 ggtacgacag actcttctgg cgagacattg acagatcaag ctgtcagcaa agtaaatgcg
     5221 tttcagcggt tttgggacgg gattgattgg gacgccattt tggctgcctt tatccaaaaa
     5281 agtttatatc tgattttttt gatcgtcctt ttttggattc ttcaccgtgt gggaaaatat
     5341 ctgattgata agtcttttaa tcagtattct aaaaaagcca ttctaaacga gagccgctta
     5401 aagactttac atagtctttt aaatactatt tttcattata cccttggttt cttttttatc
     5461 tatgcaattt tatctgctat cggtgtgccg atcggatcac ttttggctgg tgccggaatt
     5521 gcaggtgtgg caatcggtct tggtgcccaa gggttcatga gtgatgttat taccggattt
     5581 tttattatca tggaacaaca gatggatgtt ggggactaca tcaagctagc aaacttaacg
     5641 attgaaggaa ccgtcgtatc tgttggtatc cgtacccttc agctgaaagc aacggatggt
     5701 actgtccatt tcatcccgaa tcgaaacatc accacgatca gcaatctctc tcgtgcaaat
     5761 atgcaagtac ttttagatat tcggattgtt cctgaagaag gatatgacaa aatctatgag
     5821 atcattgatc gagtaaatcg tacattagct gaaaaatacc aagaagagct ccaaactgaa
     5881 ccttctatct ttggattagt ggatcttgga aacagcaatt ttgcgattcg tacggtctgc
     5941 tacgcattga acggaaaaca atatgcctta aaagaagagt tcttgagctc ctatgtcaaa
     6001 gaattgacag cttctggttt cacgattccg aacagtccaa ttgctgtcgg aaaataattt
     6061 ggacaaacga tgcaacgaat ctcactaaaa agagcgtggg acaaaagtaa agaacacttt
     6121 tgtc
//
LOCUS       DACDYI010000108         6099 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.109, whole genome shotgun sequence.
ACCESSION   DACDYI010000108 DACDYI010000000
VERSION     DACDYI010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.109"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            163..585
                     /locus_tag="IWM52_002712"
     CDS             163..585
                     /locus_tag="IWM52_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974892.1"
                     /translation="MAKVIDKHIYFYETHIDVLDEIIATHAEVKNYSEAVRFLCMNYD
                     PEVEKKVNSNQAKLNGMSKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKTISESVTTKSEVFDPTRFLKR"
     gene            605..2194
                     /locus_tag="IWM52_002713"
     CDS             605..2194
                     /locus_tag="IWM52_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974893.1"
                     /translation="MSKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPEE
                     EQELRLIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMGNGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKKNTRKPYTEIENGVSYIVPKG
                     KRRQATLDEMKTNYVHELERFSYEKNRMGMEKRNLLTRKSDLRNTLTKVVKEPVRYDR
                     HAMSLLNEVYKNLPKKRSEWHYGDENRTKLSPYTREKLDELTQYVLTKEPGYQEYIQI
                     SKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIERMDQ
                     MQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEEK
                     YHASKAYEEQQRRIQMEQERQ"
     gene            2206..2541
                     /locus_tag="IWM52_002714"
                     /pseudo
     CDS             2206..2541
                     /locus_tag="IWM52_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2689..2883
                     /locus_tag="IWM52_002715"
     CDS             2689..2883
                     /locus_tag="IWM52_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974894.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEVESERTFVVTDKEAKAYQPGDL
                     LTLDLTTKKFVDAAENYPFI"
     gene            3292..5679
                     /locus_tag="IWM52_002716"
     CDS             3292..5679
                     /locus_tag="IWM52_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974895.1"
                     /translation="MPEEVKGKSSLTLNEEEITGNSRLAQAQRKLIRLRNEYDATFER
                     LAEHQKLTNGQPMNDKRNGSSWFSQREKIENKINQLQEEIQKQENYVEKLTRQSELRK
                     MGYNKYGGLDLTIENIPRIKEEIEKFRNGKSQFSVKTIEKYQKKLVELEQLKEKSEKG
                     IANILPEAQAIIESGRVSQWKRNPTIYFVKGYRKVALELNEQGEFEVSKRYQPKTEEE
                     KRIVSELLENKMDEYGVQKQEPNHYYRVEFNENDGTMRNYQGEIVTQSLLEQIKALDT
                     HYIGKTSYHKFYFEEVKEGEVVNQLRLDVGDGLQLNASIYQKLEELCDQTLETKTEKV
                     IKNEIGSHDKKVETKKPSYQKRSTEEIKQEVKLLSQQALDAVKKYTHSPEDVKELLNF
                     MSRFPERSFRNQLLIEKQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYD
                     KGRGFVRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRV
                     FDFQLDKKGQAELQAGIQAVANTIGIQIRDMTESEVYQRELGQARGAYVHNEFTGQEE
                     IVMNTRNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEK
                     AIPYIAAWTKNGQTLDDKEVAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPA
                     EVKQEHKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQV
                     ILKGKSYGKEIQTALPSEEFVLGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            5740..>6099
                     /locus_tag="IWM52_002717"
     CDS             5740..>6099
                     /locus_tag="IWM52_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974896.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPL"
BASE COUNT         2460 a          905 c         1247 g         1487 t
ORIGIN      
        1 tggaatataa acaagataaa tttgatcaat tattaaatca attaatagat aaattttctt
       61 ttgttcaaag tcataataca aaagtattag aagaatatat ttgtcagaat aatcaaatta
      121 tcgaactttt aattgaacaa agtagaggag gagaagttta acatggccaa agttatagac
      181 aagcatattt atttttatga aacacatata gatgttctag atgaaattat tgcaactcat
      241 gcagaggtta aaaactatag tgaagccgtt cggtttctat gtatgaatta tgatccagaa
      301 gtggagaaaa aagtaaattc caatcaagca aagttgaacg gaatgagtaa agaaatttct
      361 attttaaccg aaatggtttc agagtttgcg gatctccatt tgaaagatac agggatttta
      421 attggtacaa attcaactgt ttaccaagaa gcaaaagagc gtgtggaagg caagataaaa
      481 gcaaatcaaa cgaagaaata ttctcctaag ataaagaaaa caataagcga atcagtgact
      541 acaaaaagtg aagtatttga tccaacaagg ttcttaaaac gataaaaatt agaaggagaa
      601 tgtgatgagt aaatcacctg cagtcattct cacttgtcaa tttaccttgc ccaataaaaa
      661 atctttttca acatacattg attatatgac aagagaaaaa gcgctggaag aaaaggaacg
      721 acgtacgccc gaggaagaac aggaactgcg tctaatcaag aatgctttag aggattttta
      781 tattcctgaa ggagaaactt tttcggagag taagacggat aaagaattca gcgaaacgtc
      841 gaatgaagct caatctttga tgggaaatgg aatcaacttt gacaagttag aggaacaaga
      901 ttttaccaaa tatctttctt atatgacccg tcactatgcg ttagaacaaa agaaagaact
      961 gacgcaaagt gaacaaaaag aataccaacg cttgaaccaa gcaataaaaa aatatcaaac
     1021 agagccacac aaaaaagctt caggaaacct tcctggagtt ttttctatgg cttcagacga
     1081 agtgaaagga agtgatctga aggagattaa gcaaatcttc caaagaggac aacaaaaagg
     1141 ctcaatcatt tatcaagatg ttgtctctca tgataattcg tatcttgaaa aaataggatt
     1201 gtacgatccc aaaacagata ctttagatga agaaggatta aaagcagctg gaagaaaaat
     1261 gatggagaca ttattcgaga aagagcaact gaatgaaaca ggctactgga tggcaacgat
     1321 tcaccgaaat accaaacata tccatattca ttttgcagtt gttgaaaaaa aaaatacgcg
     1381 taaaccatac actgagattg aaaatggtgt gagttatatc gtaccgaaag ggaagagaag
     1441 gcaagcaaca ttagacgaaa tgaagactaa ttatgtacat gagttagaac gtttttctta
     1501 tgaaaaaaat cgaatgggta tggagaaacg aaatttattg acacggaaaa gtgatttgcg
     1561 taacacctta accaaagtag tgaaagagcc agtacgttat gatcgacacg caatgagctt
     1621 attaaatgaa gtatataaaa acttacctaa aaagcgttcg gaatggcatt acggggatga
     1681 aaatcgaacc aaactttcac catacacaag agagaaactc gatgagttaa cccaatatgt
     1741 gttaacaaaa gaacctggat atcaagaata tatacagatt tcaaaagcat tgaaagaaga
     1801 agcaaaaagt ctttatgggg aaagcaagag agaatcaaag gatgcagaaa aaaatgctct
     1861 ctttgattta aaaaagcgca caggaaatgc cattctaaca gaactgaaga aacaggatag
     1921 tcaaataaaa cctttaatag aaaggatgga tcagatgcaa tctatttaca atgaacgatc
     1981 ctttcgaaca cttaaacagt tgccaacgaa tgaatacaaa tatagttacg aagaattcat
     2041 gaagcgcagg aaacaattcc aacaaagagt tgtcacgcaa cggagtttgc gtcaattaaa
     2101 tcgaacgatt aatgcccatg aagaaaaata ccacgcaagt aaagcatacg aagagcaaca
     2161 acgaagaatt caaatggaac aagaaagaca gtaggaggaa tagcaatgaa acaatattta
     2221 gatcaatgga aagttataga aggatcatta agagaagaac gaatcgaaca acttcccgat
     2281 tgtttataaa aagagcattt gtttcagata agagaaatgt tgagaaacga acagtttgat
     2341 ccaaatcaat ttttagtcgt tgagtaccca gctactggtg tgtaatgttg taatcatgtg
     2401 aagggtgaaa aatattttat cattcaaaaa tatgagggca agttagcgcc ttattatact
     2461 acttgggaaa tgaatgaaga ggggatcaat aacttcccgt gtaaaagcat tggagagtct
     2521 atttcactca cggaatgtta aataaataga gaaataaaag agtcctgaac ttgattgtcc
     2581 agggctcttt ttaagtgaat ttttttccgt caattcactg agtggtgcga ttcaatttta
     2641 ttatatcaaa agaaagtgag attccaaaga gaaggggggg aagaagacat gactcagaca
     2701 acgattggag ttgttgttga aaaaagcaga gacaatctag tgtttgtgac agaagtcgaa
     2761 tcagaaagaa catttgttgt cacagacaaa gaagcaaaag cgtatcaacc aggggatctc
     2821 ttaacactag atctcactac aaaaaaattt gtcgatgctg cagaaaatta tccttttatt
     2881 taagaaaaga tggtgaacaa aatggaacaa tttcaacaag taaatcctga agtacaagaa
     2941 caactgaaga aatgggaatc actttcacgt gaagcacaaa atgaaattat agtgatcttt
     3001 tctcaaaatc ctgttaattt cagaattgct ttaatgaaat atgatttaaa ggatactgaa
     3061 aatattttta caggattggg tacgcctata gggaaggctt gggaagaacg atacaaagag
     3121 ttagaaaaag aagttagtac cttcaaagca acccgtagag aatggttaga tcaatatgaa
     3181 gatagtgggg ttatgtatat tccagaaagt ttatgggaga cagctgaaga gttaggaata
     3241 atgaaaaact accccaaagt agttccaact agcgaactca ctgcaccagt tttgccagaa
     3301 gaggtaaagg gaaaaagtag tctcacttta aacgaagaag aaatcactgg aaatagtcgg
     3361 cttgcacaag cacaaagaaa gcttatccga ttaagaaatg agtatgacgc gacatttgaa
     3421 aggctggctg aacaccagaa attaacaaat ggtcagccaa tgaatgataa aagaaacgga
     3481 tctagctggt ttagtcaaag agaaaaaatc gaaaataaaa ttaatcaatt gcaagaagag
     3541 attcaaaaac aagaaaatta tgttgagaaa ttgactagac aatctgagct tagaaaaatg
     3601 ggatataaca aatatggtgg gctcgatctt acaatcgaga atattccacg aatcaaagag
     3661 gagatcgaaa aatttaggaa tggaaaatct caattttcag taaaaacaat cgaaaagtat
     3721 caaaaaaaat tagttgaatt ggaacagttg aaagaaaaat cagagaaagg aatagccaat
     3781 attttgcctg aagcgcaggc aattattgaa agtggccgtg ttagtcagtg gaaaagaaat
     3841 ccaactatat actttgttaa agggtatcgt aaagtggcat tagagttgaa tgaacaaggc
     3901 gaatttgaag tatcaaaaag ataccaacca aagactgaag aagaaaaaag aattgtgagt
     3961 gagttacttg aaaataagat ggacgaatat ggtgttcaaa agcaagaacc caatcattat
     4021 tatcgtgtag aattcaatga aaatgatgga acaatgcgga attatcaagg tgaaatcgtg
     4081 acacaatcac tacttgaaca aatcaaagca ttagacacac actatatagg gaaaacaagc
     4141 taccacaaat tttattttga agaagtaaaa gaaggtgaag ttgttaatca gttacgacta
     4201 gacgttggag acgggttaca attgaatgct tctatttatc aaaagctaga ggagttatgt
     4261 gaccaaacat tagaaacgaa aactgaaaaa gtaataaaaa atgaaatagg tagccacgat
     4321 aaaaaggtag agacaaagaa accaagttat caaaaacggt caacagaaga gattaagcaa
     4381 gaagtcaaat tgctttccca acaggcacta gatgcggtga aaaaatatac ccattctcct
     4441 gaagacgtca aagaactctt gaactttatg tcgcgttttc cagaacgatc ttttagaaat
     4501 cagttattga tcgaaaaaca atttccagga gcaacggcgt gtttaggtcg tgctgcgttg
     4561 aaaaaagaag gcatttatat caaaaaaggg gaacaaggga acaagatctt tgtcagaaaa
     4621 acggtcaaag gattctatga taaaggacgg ggctttgttc gagaaacaga ggctacacca
     4681 gaagataaaa aacgaatcga aagtggccaa atcgaagtga tcaaaaaacc ttactacacg
     4741 attgaaaaag tgtttgatat cacacaaaca cagttgaaac ctgaagacta tccgaaaatc
     4801 ttcccgaacc gtgtgtttga ttttcaattg gataaaaaag gacaagcaga actacaagct
     4861 ggaattcaag cggtagccaa cactattggg atacaaattc gagatatgac agagagtgaa
     4921 gtctatcaaa gagaattagg acaagcacgt ggggcgtatg ttcataacga atttactggt
     4981 caagaagaaa ttgtcatgaa tactagaaat acaccgacgc agcatttagc tacaagtatt
     5041 cacgaattag cacatgccag aatgcacaaa ttttcagaat tagacactgc gaccaaagaa
     5101 ctgcaggcag aaatgacttc ttatattgtg tgcaaacatt ttggaatgga cacgtcggag
     5161 aaagctattc cttatattgc agcatggacg aagaatggtc agacgttgga tgataaggaa
     5221 gttgcggaac gtgggaaaat catgaatgat gtcagccgtg tcgcaaatga attcattcaa
     5281 accatttcta atgaaatcaa tcaatacaga gaaagagaac cagagatcca accagcagaa
     5341 gtgaagcagg agcataaaaa tactttagaa acgccagaga ataagaatat tgtgcccgtt
     5401 ggttcacttt ggatcgacaa gaaagacggg aaagtaatgg aagaagatac gggtcgaacg
     5461 ttacatttga aggatgcggc gtttattgaa gatccgcagc ataatcaagt gattttaaaa
     5521 ggaaaaagtt atggaaaaga gatccaaacg gcgttacctt cagaagaatt tgtgctagga
     5581 aatccattcc gattttcact tgagacagtg tcagaagtgg caaaacgaaa aaatgaaatc
     5641 tattatgaac aacaattgtc acaaaaagaa cgtgtataaa acaagtaaaa aagactatct
     5701 gaaaaacgga tagtcttttt tcttttggga ggttttaaaa tgaccgagaa aaaaatgagt
     5761 cttattgatc gatgtaaaca gatcgatata gtggattttg ctcgaaacaa tggaatggct
     5821 gtagtcaata aaggacgaga ctatcgctta gaagaccatg attcatttgt ttttgatcga
     5881 agaaaacaac gtttttattg gaacagtcaa aatattagtg gcgacattat tgaattagct
     5941 aaactttttt tcatagacaa agagatccaa gaccctaagc aacaatttaa agcagccact
     6001 gattttattc taaagaatga agacaaaaca gaacgagtgg aaaatcttca ttttgagaca
     6061 gaaaagtaca aagatcaccc cgttgattat caaccactc
//
LOCUS       DACDYI010000109         5987 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.110, whole genome shotgun sequence.
ACCESSION   DACDYI010000109 DACDYI010000000
VERSION     DACDYI010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.110"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            287..694
                     /locus_tag="IWM52_002718"
     CDS             287..694
                     /locus_tag="IWM52_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974897.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="IWM52_002719"
     CDS             708..1109
                     /locus_tag="IWM52_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974898.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1482
                     /locus_tag="IWM52_002720"
     CDS             1111..1482
                     /locus_tag="IWM52_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974899.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1518..1820)
                     /locus_tag="IWM52_002721"
     CDS             complement(1518..1820)
                     /locus_tag="IWM52_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974900.1"
                     /translation="MLMEGKTQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(2069..2269)
                     /locus_tag="IWM52_002722"
     CDS             complement(2069..2269)
                     /locus_tag="IWM52_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9974901.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(2574..3806)
                     /locus_tag="IWM52_002723"
     CDS             complement(2574..3806)
                     /locus_tag="IWM52_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP9974902.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            4060..4629
                     /locus_tag="IWM52_002724"
     CDS             4060..4629
                     /locus_tag="IWM52_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9974903.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLC
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(4807..5247)
                     /locus_tag="IWM52_002725"
     CDS             complement(4807..5247)
                     /locus_tag="IWM52_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAP9974904.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            5405..>5987
                     /gene="map"
                     /locus_tag="IWM52_002726"
     CDS             5405..>5987
                     /gene="map"
                     /locus_tag="IWM52_002726"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAP9974905.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKE"
BASE COUNT         1984 a         1000 c         1107 g         1896 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcacct aatgaatttc aagcgagttt
     1501 gtttgttatt tttttattta atctttgtat ctttctggat caacgaaagt atactttata
     1561 tagtcataac gccgatgatc gctacgtgcg tccggcacgt cagtcacgat atcaaacaaa
     1621 aagtatacac ccttcttcat tctagttttc gcagcaggaa ttttgaaata gttcttatta
     1681 gaatagtaga gattgattaa taagctatct tcgattgcta aaaagaaaac ttctgaattc
     1741 catactttat aaaaatcttt gataaatcta ttcgaagggt caaatttaaa ccataattgt
     1801 gtttttcctt ccatcagcat aaaagttcac ctcaaaaaga gtatacgaac gaatgttctt
     1861 tttgtgaagg cggaatttaa tatactgaat aatagaaaac aagtacttgt gccaaattat
     1921 gtgccaaaaa aattcgaatt caacaaaatg taaacaaata gaaaaccttc tatattaaga
     1981 tttcctgcaa taacaaacac aacagaagac gtgtaatagt tagtcaggaa cgtacaaata
     2041 acccctgtat cctttgcggt acagggggtt attttgtata agtagacatt gtagtttcta
     2101 aactgctatg tcctaaacgt ttcgatacgc taagtatatt tactccttga taaagtaaaa
     2161 tagatgcatg cgtatgtctt agtccatcaa taaacagtcg aagagaaaat agggaaaaat
     2221 tgattactaa agaaaaactt actaggtcga cggtgtagtt gattttcata cttcaataga
     2281 ttcgaattaa acgatttcga ttggcttata ccagtcctat ccaacgaaag atttatcgtt
     2341 cttaactcta gcggtataac ttgaagcttt acgtaaagcc aatgggaatg aactggagat
     2401 gactttttat tatttttagt aatagctgat gggttaatac ttacaacttt ccttttcaaa
     2461 gtaccatata aaaaaacggt ggggttgttt agcgaaataa aggatctaag ccaatagctt
     2521 agatccttta ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc
     2581 caatcgccat ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc
     2641 atcttgtcag atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct
     2701 gctaattctt catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct
     2761 agtgctaagt gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat
     2821 tgagaaatag gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca
     2881 tctgtatcaa gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaacaact
     2941 tttccatctt taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc
     3001 tttgtacttg aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc
     3061 gtcggcatat ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa
     3121 agatggtttt taggcaatcc gataatgata tctgtaccgg gagcagtata gtgaagggca
     3181 gaaaattgtt ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc
     3241 catgctaaga cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat
     3301 gcctcaactt gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca
     3361 gccgcagcca cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt
     3421 agtgctttgc cattcgctga ttgataagac gctactcgat ggctatctac accagcaaat
     3481 gcatcgggat cggcagaaac aacgctgatc ctgcttgctc ctttttcaag ccattcatcc
     3541 gcttgatcga ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga
     3601 aattctcttt ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta
     3661 taagcttctt gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca
     3721 actgtgtgtc ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt
     3781 agaagttcat taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa
     3841 ctattagcat gataccattt aattatgatt caaacaatga ggctttaaat aaaaaaagga
     3901 ggttattttt ttaaggttaa gtttcatgat atatgttact acttgctatt tcagcttgtg
     3961 aagaaaatca aaaactaatt ttgcatatca aaaaaacatg ttacaatgaa attaatcaat
     4021 ggtggaagcg ttataaaaaa attaaggaga ttaaaaaata tggcaagaaa aaagacgatt
     4081 actagagaac agattttggc agctgcttat gaagtagctg ccaccgatgg atttacacga
     4141 tttactgcga gaaacatcgc gaacaaaatg aattgttcca cgcaaccgat ttatcttgag
     4201 tttaaaaata tggatgactt gagacaagct ctttttgagc agatctatga tcatttacaa
     4261 tatgaagtgt tcactgttga gcatacaggg aatacgatta tcgatcttgc ttttaattat
     4321 atccattttg caagaagaga gaagaagttg tatcgttcac tgtgcttaga agaatatggt
     4381 ggaggaaaag agatgcagaa tttctcttat acttactttg tgaatacagt gaaacgcgat
     4441 ccagcttatg tgaatctttc agaagagcag atcgaatctt tgtataatgg gacatggatc
     4501 atagctactg ggatcgcagc tttgatgtct tcaagcatca tccatccaac tgatgaacag
     4561 attgaaaaga tgatccaaga cagtatagat gctatcttga aattcaaaga accaatcgaa
     4621 atcgattgaa tatactcaaa agttccacaa atcagtaggt aacaagagat gtgtcaaaat
     4681 aaggaaaagt tcgtttttct tacagcgtat gcatttttaa ctcccgaaga actcttccat
     4741 gaatgcaatg aataaaagag ccatacagat tgaataaatc tggatggctc ttttattgca
     4801 agtatattaa atttttgcga ctagttcttt agcgaaagct tctaaatttt cgatatcttc
     4861 ttcttcagca ttcagatcca ctttaacatt ttcggctcct tttgatgcgc ctgtttttgc
     4921 aaaagcagca tcgaaatcat ccaccgattt gcagaattca tcgtaaaaag tatcaccaga
     4981 tccgcagaca ccgtaaattt ttcccaatag atccaattct tgcaagtctt cataaaagtc
     5041 tacgatctca tcaggcagat cgccatcatc gtatgtataa gtagctacta cgcaaatatc
     5101 tgcctcctcg aattcatctg catctacttg ggtacattcg ttgatttcga cttcgatatc
     5161 cagattttca aatgcttctg caacaatatc ggcgatttct tcagtgtttc ctgtcatgct
     5221 agcatatacg atttttgcta aagccatgat gtgcctcctc gtttataatt atttaacaat
     5281 tatgaattat accaaaacat cattgaaaag aaaataaaac ctaaagacac ctggggaatg
     5341 tcatttggct aaatgtatgc cgctatgtta aaatgagagg gatttgtaaa aaggagacga
     5401 aaatatgatt actttaaaat cagcacgtga aattgaaatg atggcagaat ctggagcttt
     5461 attagcagat gtacataaaa atctgcgtga ttttataaaa ccaggaatca caagttggga
     5521 tattgaggta tttgtcagaa actatatcga gagtcatggc ggaattgctg cgcaaattgg
     5581 ctttgaaggg tatgaatatg cgacttgtat cagtataaat gatgaaattt gtcatggatt
     5641 ccctcgtaaa aagcctttga aaaacggtga tttggtaaaa gtcgatatgt gtatcgattt
     5701 gaaaggcggc atttctgatt cttgttgggc ctatgtagtt ggagaatcta cacctgagat
     5761 cgaccgattg atggaagtaa cgaagaaagc tttgtacatc ggtattgagc aagcacaagt
     5821 agggaatcgg attggtgaca tcggtcatgc tatccaaact tatgtagaag gcgaaaatct
     5881 agcgatcgtt cgtgatttta ttggtcatgg cgttggtcct acgattcatg aagaaccagc
     5941 agttcctcat tacggagaag ctggaaaagg gttgcggttg aaagaag
//
LOCUS       DACDYI010000110         5877 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.111, whole genome shotgun sequence.
ACCESSION   DACDYI010000110 DACDYI010000000
VERSION     DACDYI010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.111"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            641..1375
                     /locus_tag="IWM52_002727"
     CDS             641..1375
                     /locus_tag="IWM52_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9974906.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1391..2560
                     /locus_tag="IWM52_002728"
     CDS             1391..2560
                     /locus_tag="IWM52_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9974907.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2576..3571
                     /gene="lacD"
                     /locus_tag="IWM52_002729"
     CDS             2576..3571
                     /gene="lacD"
                     /locus_tag="IWM52_002729"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP9974908.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3650..4576
                     /locus_tag="IWM52_002730"
     CDS             3650..4576
                     /locus_tag="IWM52_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAP9974909.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4595..5845
                     /locus_tag="IWM52_002731"
     CDS             4595..5845
                     /locus_tag="IWM52_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9974910.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         1967 a          955 c         1204 g         1751 t
ORIGIN      
        1 gtttgaaaaa tgctatttcg aggtagaagg atattatata agcacggttg actaaacgaa
       61 gtgataataa aaaaagttat tcaagaataa gaaaaattat gatacgaatt cagataaact
      121 ggagaataca gagaccttta aaagggataa gaaacaacat aaattcctct taagagagaa
      181 cgaaaggaca aagccagcaa cttgatacac gagctagtat tagaggaatt tattctcata
      241 gactcccttt tgaatacgtt tcaactttaa ttttcatgtt cataaatgat ttgaaaccaa
      301 agcaaatgcg ttgcactttt cctccgtctt ccgaaaatcg ttcaattcac tggtaagtag
      361 tcatttgata gtttgaggca aattgttgat tgatgaaggg taggagctaa aaaaagatac
      421 cagttgaaga gagaaaaaga aaaaaataga gggtgttctt gaatgaaaaa agttggaaaa
      481 ttatttttca tttcctttta ttcctattaa agtcagtgat gtcaatcata ttaacaagac
      541 taaaaatgaa aataaaaaaa agattgttct tagtattgac tcataaaaaa taccactact
      601 atacttacat tgtggttata gccagttagg agagacatcg atgcaaaata cctttcaatc
      661 aaaatcactt tattatcagc tggtagaaat attgaaacaa cgaatcgaaa acagtatggt
      721 cgctcatgac aagctgccat cagaaagaga gttgaccagt ttttatggcg ttagtcgcac
      781 gacggtgcga ctagcattgc aagaactgga aaaaatgggc tacatttatc gacaacatgg
      841 aaaaggaacc tttgtttcgg aattaaggac aaatgcgact gatctagctg gcgcatatag
      901 ttttaccgaa caaatgcggg caatgggcaa acatcctgaa accaagatat tggccttcaa
      961 gaagattgaa gcaactaaat atttagcgag tcatctgaat gttcaactag gtgagccttt
     1021 atttcaatta gaccgattgc gcttagctga tgatttgcca atgatgatcg agaaatccta
     1081 tttgccagtg aagctattta tgtccctttc tcaagaaatt ctatttctga aacctttata
     1141 taatttgttt tcagagaatt tcaatcaaat cgtacggttg gctgacgagg aattttatgc
     1201 cagcattgtc cagaaagaag atagtgagtt gctaaatatt ccagaaggat caccagtgct
     1261 ccatttagtt cgctcaacct ataatctcga gaacgaagtg attgaattta cattaagtgt
     1321 agcgcgggct gataaatttc attataagat caggcatctg cgttccacta actgaattgg
     1381 aggaaagaaa atgtttatgt tctcaaaaga agaactacga aagctaggag cagaaaatac
     1441 tacccgagaa attcgacaac aaccggaact gtggcatgag acgtttgacc gttatcaaga
     1501 aacagttgca gatttagatc gtttgtttac ggatgcgaaa caacatgctg gagagcgacc
     1561 aatccgagtc atttttacag gggccggaac ctcgcaatat gtgggagata cactaattcc
     1621 gtttcttact agtcaaggaa atacgaacca gtttattttt caaagtattg gttcgaccga
     1681 tatcgttgct acgccaaaag aatacctgat tcctgatgag acaacgatct tggtctcatt
     1741 tgcccgtagt ggaaatagtc cagaaagtct agccgcagtt aagatggcca atcagctggt
     1801 caacaagatc tatcatatcg tgatcacctg tgcaccagac ggattattgg ctacacaaat
     1861 gcctacaaaa aaagaaaatc tagttttact aatgccaaac aaagccaatg acgctggttt
     1921 tgctatgaca agcagtttca gctgtatgac gctgactgcg ctacttattt ttgatcaagc
     1981 aagcaatcag caaaaagaaa gatatgttca tcaattaagc cgactaggag aagaagtcat
     2041 ccaacgagaa tcagagatcc aagggtatct ggcatcagac tttcatcgca tcatttactt
     2101 aggttcggga agcttaggcg gattgactag agaagcgcaa ttaaaagttc tcgagttaac
     2161 tgctggaaaa atcgcaaccg tatttgatac atctttagga ttcagacatg gtcccaaatc
     2221 ttttgtcaat gagcagacat tggttttcct gtttgtcagc aatgactcat atacgcagaa
     2281 atacgatgta gacatgctgg aagaaatcaa aaatgacggt attgcccaac gcgttgttgg
     2341 aattggtcaa aagggttcat tgtttagtgg tgattccttt gaattagttg cagattcgtt
     2401 actgccggat ggctatcttg ctttagcaga tatcatggta gcccaaacga tcgcattact
     2461 ttgttcggtg aaggttggta atacaccaga tacaccatcg ccaagtggaa cagtcaatcg
     2521 tgtggtcaaa ggcgtaacga tttatccata tgaaaaatag gaaagagtgt gaagcatgtt
     2581 agaatggtcg gaaaataaaa gacaagcaat ggaacgtcta tcgacgccag aaggcattat
     2641 taccgctttg gcgatcgatc agcgaggcgc gctgaagaaa atgatgtcgg ctttagggat
     2701 ggaagcgaca aaggaaaaaa ttgagcagtt taaaacacta atttcccaag aattgactcc
     2761 ttatgcctca gcaattttat tggatcctga atacggactg cctgctgcac agaagcgtgc
     2821 agcagcgagc ggtctgctgt tggcatatga aaaaactggt tatgatcaaa ccgtacccgg
     2881 ccgcttgccg gatctgctgt cggactgttc agttctgcga ttgaaagagt taggtgcgga
     2941 tgcggtgaaa tttttactgt attacgatta tgaagaagca gaggaaatca atcggcaaaa
     3001 gcaagttttt gttgaacgaa ttggaagcga atgcatggct gaaggactgc cattttattt
     3061 agagttattg tcatatgatg ctaaaaatgt agatacgaaa tcgctttcct atgccaaagc
     3121 aaaaccgcgc aaagtgatcg aaatgatgaa agaattctcg aaaacccagt ataaagtaga
     3181 tgtcttaaag gttgaagtac cagtaaacat gtcatttgtt gaaggaaatg caacagggga
     3241 gatcgcctat acacgtcaag aagctgccgc ttattttaaa gaacaaagcg atgcaacaga
     3301 tttgccgtat atcttcttaa gtgctggggt atcagcggca ttattcatgg agacattgcg
     3361 ttttgccaaa catgctgggg cacgattcaa cggtgtactc tgtggcagag caacttggaa
     3421 agatgcagtc gctcctttcg ttgaaaaagg agaagcggca actttagaat ggctgacaga
     3481 acaagggaca caaaacattc gtcaattgaa tgaagtgata agagaaactg ccatcccatg
     3541 gtatgagaaa gtaagtgaaa gcacttgtta atgtttctaa tatattagta gtgataagag
     3601 atatgtgtga ttataattta tcattattga aactgggagg atattttata tgaataaaag
     3661 attgttagat tattcagctt cagatttttt gaaagctaag ccgatggaat taaagcaggc
     3721 aattttggct tctgaaggta gaacaattat ggcagaaaat gtgccaagta gctgtcctta
     3781 catgggaaaa tcagtgacaa atgcagaatt ggagagagct gctggagctg atttaatttt
     3841 gtttaatgca ctagatcttt ttaatcctca aatttcaggt gtacctttaa ctctaaaaga
     3901 gaatccaata atctggatta aaaaagcttt aggaagagct attggtgtaa atttagaacc
     3961 tgtagacaaa gaagttttga tgagtgaaaa tcgaactgaa atttcaagtg gtagaatcgt
     4021 ttctcctgag actttaaaaa aggcaaatga attaggcttt aattttattt gtttaactgg
     4081 aaatccagga acaggagtat ctaatagaac tattgagaaa gcaataacat tatccaaatc
     4141 aatttttaat ggattaatta tcgctggaaa aatgcatggt gctggtgttg atgaaccaat
     4201 tatgaactta gatatagcaa aaagatttat taaaacaggt gtagatattt tattagttcc
     4261 tgctccatac actgttccgt atttcatgga aagtgatttg cgaggaatcg ttgattatat
     4321 aaaagatttt aatagaaata aatctattga ggaaaaagtt ctagtactaa cggctaatgg
     4381 aacaagccaa gactcttctg accaagaaac tattaaaaaa attgcattag catcaaaagc
     4441 atgtggagct gatattcaac acatagggga ttctttaaat ggtatttgtc ttccagaaaa
     4501 tatttttgct ttgggtcaag caattcgggg atatcgtcat caaattatta tgttaggaaa
     4561 atctaattat cgttaattaa ggaggaaatg aaagatgatc gacaaagttc aaaaaatagc
     4621 tgcaaaaatt agtaatcaaa gacatctact agctttaaga gacagcatgg ctgcagttat
     4681 gccattaatc attattggtt ccttttttat gttattagca aatttcccga tagaaggatt
     4741 tacaaaatgg ttatctgaaa ttggagtaca atcctttttg aacaaagcat cagattcaac
     4801 atttggtatt attggattag ctgttaccta ctctttggga cacaatctag caaagactta
     4861 tggtgtagac tcattatcag caggattatt atctttgagt ggatatgttt tacttacacc
     4921 tttattggat gcagatggtt ctataggatt tccaacaaaa tactttggta cctcgggtct
     4981 ttttgttggt attattgtag cacttgtatc tacagaaatt ttccggtggt ttgtacttaa
     5041 aaatattaca attaaaatgc cggcaagcgt tccaccaaat gtaagccgag ctttcgttgc
     5101 tattattcct ggcttttttg tcgttctact atggtttatt gttgttgtaa tttgttataa
     5161 acttggaatt gaaaatgttc atgctcttat tgcagataca ctcgcaaaac cactatcttt
     5221 actgacgaaa actttaccag gaattatatt ggttatactt attcaatgtt tcttttggat
     5281 gtttggtatt catggtgcac aggtaacggg gccaataatt gaaccgttat tattgcaaaa
     5341 ctctgatgta aatagaattg cttatcaagc tggaaaagaa cttcctaaca ttataactta
     5401 tgaattctta tataactttg ttttttctgg tggtgcaggt tgtttatttg cacttgccat
     5461 tttaacattt ttcttttcaa agagtcagga aaataaaact ttaggtaagt tatcaatcgc
     5521 tccagtaagt tttcaagtag cagagcccct tctatttggg tttccaacta tattaaatat
     5581 aaaaatgtta attccatttg taactgcacc agtagtaaca acattaatta cttatttttc
     5641 aatgagtatg ggattagttg caaaacctgt aggagcaaca atcccatgga caactccacc
     5701 gataattgcc ggatttttag cttctggtgg acgaataagt ggtgcagtga ttcaagtaat
     5761 aacaattgca ataaatgttt tgatatacta tccattcttt aaattagatg ataatgccaa
     5821 gctaaaaagc gaaaaaaatg attagaacta cctatatgtt tctaactgta gttaagt
//
LOCUS       DACDYI010000111         5595 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.113, whole genome shotgun sequence.
ACCESSION   DACDYI010000111 DACDYI010000000
VERSION     DACDYI010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.113"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..2093
                     /gene="acm"
                     /locus_tag="IWM52_002732"
     CDS             <1..2093
                     /gene="acm"
                     /locus_tag="IWM52_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAP9974911.1"
                     /translation="VYADAGRDISSNVTSLTVDPTNITDGGNIKVKFSFDEKKQNIQP
                     GDYLWINWPSEGNIRGEGFQKEIPLMIENKNVGTLTVRKDSAQVVFNENIKNLDSVEG
                     WGQFEIQARNVTDTGEENTGPFTVTSGDKTATVNVTKPASGSSSSVFYYKTGDMLPED
                     TKHIRWFLNINNNGTYVEQPVKISDEIQSGQRLDPSTFEINQIHLGEQKVYRGEEGIQ
                     QFLQDFPGATFNFSVTDNYIEITIPKNFVNLRKIMVSYKTIIENPEQINFENHSEAWF
                     KEFNKPAVDGESFNHTVKNISASGGVNGTVRGELKIFKYINDTEIGIPNVTFELRRAD
                     EQPIQGQSSILLTSNEQGEASIKGLQVGDYVVKEKEAPNWIDFDPLSSNELKFSINEN
                     DTEGVSLPIYNKKKVTNITATKIWNGGTTPRPSIYFKLFRASTNNWEPVPDAETKRLD
                     NGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGNDYVPSGYQKIENGLVVTNENKEVIS
                     VSGQKTWEDNENQDGKRPTTITVNLLADGQPIQHKEVSEKDDWRYRFTNLPKYRDGQE
                     IIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNISIPGSHITPWKNFPEKTEREKIRNL
                     FSQKLQIFAHTGESSEQRSNYISKRAVPKQITNKSKSILPKTSSKKSSFAVIIGLLIV
                     TICAGIFLQKYKKV"
     gene            complement(2365..2850)
                     /locus_tag="IWM52_002733"
     CDS             complement(2365..2850)
                     /locus_tag="IWM52_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAP9974912.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            3330..3971
                     /locus_tag="IWM52_002734"
     CDS             3330..3971
                     /locus_tag="IWM52_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9974913.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            3990..4625
                     /locus_tag="IWM52_002735"
     CDS             3990..4625
                     /locus_tag="IWM52_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9974914.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4643..5458
                     /locus_tag="IWM52_002736"
     CDS             4643..5458
                     /locus_tag="IWM52_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9974915.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
BASE COUNT         1969 a          912 c         1156 g         1558 t
ORIGIN      
        1 atgtctatgc ggatgcaggc agagatatca gcagtaatgt cacttcgttg accgtggacc
       61 caacgaatat tacagatgga ggaaacataa aagtaaaatt ctcgtttgac gaaaaaaaac
      121 aaaatatcca accaggtgat tatctttgga tcaattggcc aagtgaagga aatattcgcg
      181 gtgaaggatt ccaaaaagaa atcccattaa tgattgaaaa taaaaatgtt ggaactttaa
      241 cggtcagaaa agattctgcg caggttgtgt ttaatgaaaa tattaaaaac ctagactcag
      301 tagaaggttg ggggcaattt gagatacagg ccagaaacgt aaccgatacc ggtgaagaaa
      361 atacgggacc attcaccgtg acgagcggtg ataaaacagc tactgtaaat gtgacaaagc
      421 cagcttctgg ttcttcttct agcgtttttt attataaaac aggagacatg ctacctgaag
      481 atactaagca cattcgatgg tttttgaata ttaacaataa cggcacttat gttgaacaac
      541 cagttaaaat cagtgatgaa atccaaagtg gacaaagatt agatccaagt acgtttgaaa
      601 ttaatcaaat acacttaggt gaacagaagg tttatagagg agaagaggga atccaacaat
      661 ttttacaaga tttccccggc gctacattta atttttcagt aactgataat tacattgaaa
      721 taactatacc aaaaaacttt gtcaatctta ggaaaataat ggtgagttat aagacgatca
      781 tagaaaaccc agaacaaata aattttgaga atcattcgga agcttggttt aaagaattta
      841 ataaacctgc tgttgatgga gaaagtttca atcatacagt taaaaatatt tcagcttctg
      901 gtggagtcaa cggtacagtt agaggcgaat tgaagatttt caaatatatc aatgacacag
      961 agattggtat cccaaatgtg actttcgaat taagacgagc agatgaacag cctattcaag
     1021 gacagtctag tatcctgttg acttcgaatg aacagggaga agctagtatc aaaggcctgc
     1081 aagtaggaga ctatgttgta aaagaaaaag aagcacccaa ttggattgat tttgatccac
     1141 taagttcaaa tgaactcaaa ttctccataa acgaaaatga tacagaggga gtcagtttac
     1201 ctatttataa caagaaaaag gttacgaaca tcacagcaac taaaatttgg aacggtggaa
     1261 ccactccaag accgagtatt tattttaaat tattcagagc aagtacaaac aattgggaac
     1321 cagttcctga tgcagaaaca aaacgattag acaatggaat aacttcagtg acttgggaag
     1381 atatccaaca gtatgatgat agtggaaacg aatatacgtt taaagtgcaa gaagtagatc
     1441 aaaacggaaa tgattatgtc ccttcaggtt atcaaaaaat tgaaaacggg ctagtcgtta
     1501 caaatgagaa taaagaagtc atttcagtct caggacaaaa aacatgggaa gacaatgaga
     1561 accaagacgg caaacgaccg acgacgatca cagtaaatct attagccgac ggtcaaccga
     1621 tccaacacaa agaagtaagt gaaaaagatg attggcgcta tcgtttcaca aatctgccaa
     1681 aatacagaga cgggcaagaa atcatctata ctgtaacgga agacaacgtg cccgaataca
     1741 gtacgacgat tgacgggtat aacatcaaaa acagttatac accggaaaaa acaaatatat
     1801 ctataccagg tagccatata acaccgtgga aaaattttcc agagaagaca gagagagaga
     1861 aaattagaaa tctattctct caaaaattgc aaatatttgc tcatacagga gaatccagcg
     1921 aacaaagaag caattatatt agtaagagag ctgtacctaa acagataaca aataaatcaa
     1981 aaagcattct tcctaaaaca agtagtaaaa aatctagttt tgctgtaatc attggtttat
     2041 tgatagtaac tatctgtgct gggatattct tacaaaaata taaaaaagtt tagttagaat
     2101 ataatatttt ttgttaaata tgactgaaat caaaagaata aaattccgat ttataatcga
     2161 aaaaagaagc atttttatat atgcttcttt tttcgtagaa tcaataaata tggatttggc
     2221 taactatgga agaatactcg tattagtttt cagcgttcat actagttgtt acattgtttg
     2281 attggccaaa agaatttctg atatattttg tgctaaataa agaaaaagac gcttgaattg
     2341 ggaaataaga aaactactct atcattacaa attttcataa actggcagat gttctaagta
     2401 aacaagtatc ttttcaaagt cggaaaaatc cataatcaac gtagtggtat tgatatttgg
     2461 atgaaagcct acggtatctt gacgatcaat agcagcatcg atcacgactt ggattttttt
     2521 ctctttgtcg tgcatcaatg ctaatggagt cactgtacct gctggaacgc ctaggaggtc
     2581 aggaagctgt tcttcagaaa caaaagataa gcgcttttct tttaattgtt cagctagttg
     2641 ttttaaatcg gcgtgctttt catcaggcaa gatgacaaga tagatttgtt ttccttttct
     2701 tgatttcaat aatagatttt ttacttgcgg accaggtagg gaaaaaggga catttttgac
     2761 tgacgtaatc gcaggatgat cgactttttg aaaagagata cccagttttt ttagtagttc
     2821 ataagcttct tgttcggttg cacgactcat cgcgcatcac cctccttttt ttattagtgt
     2881 atcataaaaa agagttgaca aatgaaaaat aatcatgtaa attacacgta aagttaaaac
     2941 aaatgacatc gaaaagaaga gtagccgtta ggtgttttta gagagtctct ggttggtgga
     3001 aagaggcagc agttaaccgt gaaacacatc ttggagtcga ttttctgaag cgtatagtag
     3061 gaaaatccgg ggagcccgtt acagctgcgg tcgttgctag atgaccagat aaggctggct
     3121 tttccgtgag gagtcggtaa aaaaaggtgg aaccacgtga ttaacgtcct ttgatcttcg
     3181 gatcaaagga ctttttgttt tcatctgaac ggcaacgact gtgtgaggtt ttttctgcaa
     3241 ggaaaaaata aacagaggtg gaaccacgta taatacgtcc tcttggcatt tgccaagagg
     3301 atttttttat tttaaaggag gaaaagaaga tggagtattt gattgaagtt atgccgcaat
     3361 tattagtagg agcactcaca acattgaaat tatttttatt taccttgatt ggctcattac
     3421 cattagggat tgttttagca ttaggattgt ctttgcattt taagccgcta cgctacttgc
     3481 ttcaaacata tgtatgggtt atgcggggaa caccgctgtt gttgcaactg atttttattt
     3541 tttatgggtt gccgacgatt gggatcgttt ttgatcgttt cgaagcagca ctcattgcct
     3601 ttatcttaaa ctatgcagct tattttgcag agatctttcg tggagggata caatcgattc
     3661 ctcaaggtca atatgaagca gcgcaagttt tgcaattaag tccctttcaa acggtgacga
     3721 aaatcgtcat cccacaagtg acaaagattg ttttaccttc tgtagggaat gaagttatca
     3781 acttggtcaa agattcttcc ttgatctata tattaggttt aggtgacgta atgcgtgctg
     3841 ggaaaatcgc gatggaacgc gacgcgactt tactgccctt gctaggcgtg gcaatcatct
     3901 atcttacatt gactggtatt ctaacaatcg taatgaaaca agttgaagga aaattaaatt
     3961 attaccgcta actgatagga ggaagaaata tgattgaatt gaagaaagtg aacaaagcat
     4021 ttggtgaaaa aaaaataatc aaagatttga atctaatcat tccagatggg aaaacattag
     4081 ctatcgtagg tccttccggt ggcggtaaaa ccacgctttt acgtatcttg gctggcttag
     4141 aaagttctga cagcggagaa ttttatttag acggtgtacc gttcaaccca ttcgagctta
     4201 aaatgaaaga acaagttgta ggagtcgtat tccaagactt ccagctattt ccacatctga
     4261 atatttttga aaacattacc ttagcaccaa aaatggtatt gaaacagaca aaagaagaat
     4321 acaccaaaaa agcagaagaa ttagcggctt cattagggat tagtgattta ttgaatatct
     4381 atccttatca gctttctggc ggacaaaaac agcgggtagc gattgccaga gcattggcta
     4441 tgaatccacg ggtattggct tatgatgaac caacaagtgc tttagatcct gaattacgcc
     4501 agcaagtcga gcgtttgatc ctatcattga aagaagatgg ggtaacccaa attattgtca
     4561 cccatgattt agtatttgca gaaaatgtcg gtgatcaatt actaaaagtt tccccaattc
     4621 attaagaaag gagaagatag agatgaaaaa aaccagtttt ctattcgctt tgatcgcagg
     4681 tcttcttctt tttgcaggct gttcgaacaa aaaaacttca gcagataatt gggcgactat
     4741 caacgaagag aaaaaaatcg ttataggttt agacgataca tttgttccta tgggatttag
     4801 agataaagac gggaagctca caggatttga tattgattta gccaaagcag tctttgaaca
     4861 atatgggatt acggtagact ttcagcccat tgactggtca atgaaggaat ttgaactgaa
     4921 caatggaacg atcgatttga tatggaacgg ctattccaaa acgccggcaa gagaaaagaa
     4981 agtgcaattt acaaaacctt atatggaaaa cgatcaagtg ctgatcacac ctaaagaatc
     5041 aggaatcaca aactttggac aaatgaataa taagagatta ggtgcgcaaa atgggtcatc
     5101 tggttatgat gtatttacga aacaaccaga agtattaaaa gagatagtca aaaaccaaga
     5161 tgctgttttg tatgatagtt ttaatgaagc attgatcgat ctgaaaagca gacgggtcga
     5221 tggtctgctg atggataaag tatacgcaga ctattatttg aaacaaagaa acgaactaga
     5281 agacttcaac ataacaaaag gttcttatga gagtgaggat tttgcagttg gtgcacgcaa
     5341 gtcagatagt acattagtga aaaaaatcaa tgaagcccta gatcaactag agaaaacagg
     5401 tgaattccaa aaaatatccg ataaatggtt tggcgaagat gttacgccga gaaattaaca
     5461 aataggggac tgtgacaaaa gacgtgtcac agtccctttt tccgaataaa cagtgttctc
     5521 ctgccaactc cttgtgactc ggagctgaat ggcaggctca caacctcttg gtaaacggtg
     5581 ttcaaaagcc agttt
//
LOCUS       DACDYI010000112         5112 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.114, whole genome shotgun sequence.
ACCESSION   DACDYI010000112 DACDYI010000000
VERSION     DACDYI010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.114"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..209
                     /locus_tag="IWM52_002737"
     CDS             <1..209
                     /locus_tag="IWM52_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974916.1"
                     /translation="SIKEEAQEQARKEYEQQLEELLNRTNQSTTESSMENSKESDPSK
                     AKEGSSDSTVQSNTSESSESNEVK"
     gene            209..2038
                     /locus_tag="IWM52_002738"
     CDS             209..2038
                     /locus_tag="IWM52_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP9974917.1"
                     /translation="MRVRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGGFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYQDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNIL
                     STAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHQGKK
                     FHEERVKVGYRGFCEINFDCHLEQGDHVCLKYDHRGKEYVAWYEFSQRVLKDEEGNIV
                     YRKKRGETHLPELEKEAVLTLDEDKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHII
                     VSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNSVTCLA"
     gene            2733..4649
                     /gene="ltrA"
                     /locus_tag="IWM52_002739"
     CDS             2733..4649
                     /gene="ltrA"
                     /locus_tag="IWM52_002739"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP9974918.1"
                     /translation="MERDKTMTNTKGISLKNKQLRHAEYYDMTETFDELHAMSVAKSN
                     FKNLMKFIIDDNNIFLAYRNIKKNKGSATAGYDGVTIKDIEKLACGDFLTIIKRRFKD
                     YRPRKVKRVEIPKPNGKLRPLGIPSIWDRIVQQCILQILEPICEAKFNKHSYGFRPNC
                     STENAIADCLARINQSKMNYVVDIDIKGFFDEVNHTKLMRQIWTMGIRDKQLLVIIRK
                     MLKAPIVMRNGNVVFPTKGTPQGGILSPLLANICLNEFDWWIANQWEERTVKELKHHY
                     RKNGAWDKGNIYSKLRSHTSLKEMYLVRYADDFKIFCRTGEEAQRAFYATQKWLRERL
                     KLPISMEKSKVTNLNKAESEFLGFSIRLHKKGKKKVAFTYVCRKAEENIKNKLKQQIK
                     KIQYAGAKKNRHRAIGDYNMMVIGIHNYYRIASHCSVNFTEIYRSVRTVIEARLKARG
                     MTKNGIYGQDHEGYRPYLKSKQMRYIEGFPILPIGYIKKRTPLNKRRNLNKYTKEGRK
                     LIHQEQQAIESWKIKYLVKGFSNNKKYTVEFSDNKISKFISQKGKCAITGNELLMEEI
                     ECHHINPYHLSKDDSYKNLVIISREIHRLIHATSEETILKLLRLLKLNHYQIRKLNTY
                     RKKAANFPISIKMN"
     gene            4766..5112
                     /locus_tag="IWM52_002740"
                     /pseudo
     CDS             4766..5112
                     /locus_tag="IWM52_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
BASE COUNT         1905 a          831 c         1032 g         1344 t
ORIGIN      
        1 cgtcaatcaa ggaagaagcc caagaacaag caagaaaaga gtatgaacaa caattagaag
       61 aattattgaa tcgtaccaat cagtcaacaa cagaatcaag tatggaaaat agcaaagaga
      121 gtgatcctag taaggctaaa gaaggatcaa gcgattcaac tgtacaatca aacacttccg
      181 aaagttcaga atcaaatgag gtgaaataat gcgtgttaga gaaacaacga gacgaaaaga
      241 caaacgattc aatttagaaa agaataggta tctttctcat ttctatcgct atactccttc
      301 aggagaagag ctaccagaaa aacatgacaa acgtatttct caaaagattt tttatagtgg
      361 cttagcttat cttcctttaa actatgttgt tacaagaata ccagaagcat ttcatcgatt
      421 tgagattatt ccaggaagtt tacaaggagt gaatattttt cagttacaag aaatgccgca
      481 gttattccat tttgattcaa ctggtttttg gaatcctttg accccaacaa caagtttagg
      541 cacacgttta gttgtttcta cgatttgtgt tgttccagct gtacttatgg gattgaaaat
      601 ggacatgtgg tttcgtcctc tagcagatgg gcaaaaagga gataaccgat taaaaacgtt
      661 aaaggaaatc caacaaattt atccgcaaat accagaatca aagggaggtt ttcctggtta
      721 tggcggaata ccaattactc attataaaaa gtactggtat attcagacgg atacagtaaa
      781 tacttgtatt gttggtactt cccgttcagg gaaaggacag atagaagttt tagcaaccat
      841 tgacaatttg tctcgtgcag aaagacaaag ttcaatggtt gttaatgatc caaaaacaga
      901 attatatgtt gctagtaaag atgaactaga agctagaggg tatgatgtgt atgcatttaa
      961 tatcaccgat ccactgcaat caatgagtga taatccattg gcactcatcg tgaaatactg
     1021 gaagcgagga gacatagaca cagcgacaca gttaaccaat actttcacga atacgattta
     1081 ttatgatgcc aacgcttcag acaataaata ctttaacgaa aatgcgcaaa aagctgtcaa
     1141 tgcgcttatt tttgcgttat tggaacaagc ggatcagaca ggggattact ccaaattaac
     1201 accgaataat ctcgtcgaac ttctttctga gattggtggt tttaattacc aagatcctga
     1261 taatgaattc aagcagctca atgctttaga tgaatttatg aatcagttac cacctggaaa
     1321 catagcaaaa aaacaatatg gggcaacaaa aattggttct gataaagcga aaggcaacat
     1381 tctatcgaca gcgatcacag gactaaatcc atttacactg acgaaaattg cgaaaatgac
     1441 ttctcaatca accattgatt ataagtccat tggttttccg aagtatttgg atctcaaact
     1501 acacgagagt ctgttaaacc agcgaattgt tgtagagttt tatcatcaag gaaaaaaatt
     1561 tcatgaggaa cgtgtcaaag taggctatag aggtttttgt gaaattaact ttgattgtca
     1621 tttagaacag ggagatcatg tttgcttaaa atacgatcat cgaggaaaag aatatgttgc
     1681 ttggtatgag ttttctcaaa gagtattgaa agacgaagag ggaaatatcg tttatcgaaa
     1741 aaaacgagga gaaacccatt tgccagaatt agaaaaagaa gccgttctaa cattagatga
     1801 ggataaaagt aacttattag tagagtctat tcggatgcac tataccgata aaccaatggc
     1861 tatttttatg ctcacacctg attatgatcc atctaaccat atcattgtta gtatttttct
     1921 tagtcagttg tataaagaat tatctacgca atgtgttaag actaaaggag ataaatgtca
     1981 ccgtaggatt cattttattt tagatgaatt tgggaatagt gtgacctgtt tagcctaagg
     2041 ataaaagagg ctagttccaa cgaacattgg aataacttta ctatcgagaa atcaaatgac
     2101 ggggtaacgc cctgaagggt ttccactgat actgcgacat gcgttagtag gatgtgcatc
     2161 caaacgcgta tgaagctcgc tgaagtcggc agaagggtac cgtaaggaaa gacacgatac
     2221 gtatctacca cgcaagtgct ctagaggtag ggtatgtacc tgatatgtcg ggtgtcctaa
     2281 aaagttcaat aaggtgagaa tgatttgatt atctgacaaa cttgcgaatg gaatgtctga
     2341 aacgaataac tcatagaaat gtgaggggga agggggatac gtcctctttc cattgactcg
     2401 aggttgagta aaaatgtgtt tctatgaaaa accttgcatg gttaaaggct atggagtagt
     2461 caacaggcaa aagcaagcac ctaagttgaa atctatggat agatagtaag gaacagggga
     2521 agcatctagc acggagggag cataccgaag atgtgtttct aaggaaaaac cgaaatcggt
     2581 tataacttag gtgaatagat gtgaaagtag agccacgacc agtgaaaatt tcgtaatgaa
     2641 attggaggaa caggctcaag tcgttattta taatataaga aacctattta aaatcatgga
     2701 ttctcgtaag actaaaaagg tcacgaacca gaatggagcg tgataaaacc atgactaaca
     2761 caaaaggaat tagcttaaag aacaaacaac tcagacacgc cgaatactac gacatgactg
     2821 agactttcga tgaattgcac gcaatgagtg ttgcaaaatc aaattttaaa aatctcatga
     2881 aattcattat tgatgacaat aatatctttc tagcgtatag aaatatcaaa aagaataaag
     2941 gaagtgcaac ggccggatac gatggtgtca ctataaaaga cattgaaaaa ttggcatgtg
     3001 gtgacttttt aactattatt aaaaggcgat ttaaggatta cagaccaaga aaagttaaaa
     3061 gagtagagat cccgaaacca aacggaaaac ttcgaccttt aggaattcct tcaatatggg
     3121 acaggatagt ccagcaatgt attctacaaa ttttagaacc aatatgcgaa gcaaaattta
     3181 ataagcatag ctatggattc agaccaaatt gttctacaga aaacgccata gcagattgtc
     3241 ttgctagaat caatcaaagt aagatgaatt atgttgtaga tattgatata aaaggattct
     3301 ttgacgaagt aaaccatacc aaacttatgc gtcaaatatg gacaatgggg attcgagata
     3361 aacaactcct tgtaattatt cgtaagatgt taaaggctcc gattgttatg cgaaatggca
     3421 atgttgtttt tccaaccaag ggtactccac aaggagggat tttaagtcca ctgcttgcga
     3481 atatatgcct gaatgaattt gattggtgga tagctaatca atgggaggaa cgaactgtca
     3541 aagagctgaa acaccactat cgaaaaaacg gagcatggga taaaggaaat atctattcga
     3601 aattaagaag ccacacaagt ttaaaagaga tgtatctggt acgctatgca gatgatttta
     3661 aaattttctg ccgaacagga gaagaagccc aaagggcttt ttatgccaca caaaaatggc
     3721 tcagagaaag attaaagctc ccaatttcaa tggagaaatc taaagttacg aacctaaata
     3781 aagctgaaag tgaattcttg ggtttttcaa taagactcca caaaaaggga aaaaagaaag
     3841 tggctttcac gtacgtttgt cgcaaagcag aagaaaacat taaaaataaa ctaaaacaac
     3901 agattaaaaa aattcaatac gctggagcaa aaaaaaatag gcaccgagca ataggtgact
     3961 acaacatgat ggtcatcggt atccacaatt actatcggat agcttcacat tgtagtgtca
     4021 atttcactga gatttataga agtgttagga cagtaataga agcaagactg aaagctagag
     4081 gaatgacgaa aaatggaatc tatggacaag atcatgaagg ataccgtccg tatctaaagt
     4141 ctaagcaaat gagatatatt gaaggttttc cgatacttcc aattggctat attaaaaaaa
     4201 gaactccact caacaagcgg agaaatctaa ataaatatac caaggaaggg agaaagctca
     4261 ttcatcaaga gcaacaagct atagaaagct ggaaaatcaa gtatcttgta aaaggtttta
     4321 gtaacaataa gaaatacacg gtggaatttt ctgataacaa aatatctaag tttatttccc
     4381 aaaaagggaa atgcgctatc acaggaaacg aacttttgat ggaagaaatt gaatgccatc
     4441 acattaaccc atatcatttg tccaaagacg atagttacaa gaaccttgtc attatctcta
     4501 gagaaatcca tagattaatc catgcaacta gtgaagaaac tattctgaaa ctgcttcggt
     4561 tactgaaact aaatcactat caaattagaa aactaaacac gtatcgaaaa aaagcagcta
     4621 attttccaat atcaatcaaa atgaattaga taggaaacaa attatattgt aattaacgat
     4681 ggaacgccgt gtgcggtgaa agtcgcacgc acggtgtgga ccgggggaaa agacggagat
     4741 aatatcaaac tcttacctat cggaattgcc accaatggat aatatggaag gaatcatgac
     4801 agtcacagct ggtagaaaca tgttgtggga tttgtttatc caatcctatc agcaacttta
     4861 tgcgaagtat ggggaacaaa acgcagcgat tatcaaagaa aattgtcaga cacatattta
     4921 tattatgtcg acagacgata ataccataga agaattttcc aaaaaagtgg ggaataagac
     4981 ggttgaacaa gagaacagta ccatcaattc attaggtatg aatactcata tcaatcgcaa
     5041 tgttgattca gatcgcatct taactcctga aagaatttct acttttttag aaggtgaaac
     5101 gattgtactc gc
//
LOCUS       DACDYI010000113         5061 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.115, whole genome shotgun sequence.
ACCESSION   DACDYI010000113 DACDYI010000000
VERSION     DACDYI010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.115"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            179..775
                     /locus_tag="IWM52_002741"
     CDS             179..775
                     /locus_tag="IWM52_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP9974919.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            899..2698
                     /locus_tag="IWM52_002742"
     CDS             899..2698
                     /locus_tag="IWM52_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP9974920.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2976..3188)
                     /locus_tag="IWM52_002743"
     CDS             complement(2976..3188)
                     /locus_tag="IWM52_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974921.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(4052..5011)
                     /locus_tag="IWM52_002744"
     CDS             complement(4052..5011)
                     /locus_tag="IWM52_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9974922.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSAASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT         1493 a          876 c          887 g         1805 t
ORIGIN      
        1 tttaaaaaaa acaagttatt aaataagtct ttagttggat gtttttttga gatcaatctt
       61 ttatacttat gatgaactta caaaattgtt tgttttctgt catatcaaaa aatggcgcag
      121 ttagttgctt ttctagtaaa gtatagttaa caaagaacct ctaaggagtt gatgaataat
      181 gaatattgct gcctctcgaa aactgataaa tttcatatcc attatcggat taggcctatc
      241 aatcgcgctg acgatttatt ttattaattt aggtgttttc aaagatttga atgcgttaag
      301 aggattagta ggtgattcca tcattttagg accgattatt tttgtcttca tccaaatcct
      361 gcaagtagtt attccaatca ttcctggagg gatcagtact gcagctggtg tcctgatatt
      421 tggcccttat gctggattta tctataacta tattggaatc tgcattggtt cgattatcat
      481 ttttctgcta gggcgtcgtt atggaaagcc attcatactt tccatgatta gcgataagac
      541 atataacaaa tatattggct ggctggataa tcaaaaccgt tttgagaagc tgtttgctct
      601 agctattttt cttccagttg ctcctgacga tgcgttatgt ttgatggctg gattgacaaa
      661 catgtcagtg aaacgatata cactaatcat tttgattgct aaaccactgt cgattttctt
      721 atatagtatg gccttgattt atggaggtca atatctaagt ggtcttcttg gataatattt
      781 attttgtaaa attagtcatt gattgacaat gttaaatctg ggaaagggtc tgcaaaacac
      841 actgcaccct ttcctttttc attagatttg cgctataatg tgaagagcag gtgataggat
      901 gaattatttg aaacgtagct taggtaatgc ttgggacttt ttaaaaggaa cacaagctta
      961 tttccgtgat gtcttgctga tgcatggctt tattttattt atttgtttac ccttattgag
     1021 cagtacgacc cgttttattt tacaccgagg ggcaattgac tatttgtctt atgacaatat
     1081 tccttcaatt ttttctcaac atcctggggt tctactctcg ttaatcgtag ttttgatgac
     1141 gattttactg ttagtttatt tcgaatttac ttttcttttg atgagtgttt tttttattaa
     1201 aaagaaagaa ccaatctctt tgaagcaact cttacacttg acgatacttc agctgaagaa
     1261 agtgagaccc attacttttt tgtttttcct tgcttatttt ttattgattc taccgatcag
     1321 cggattaagt tttaattctg atctactgtc aaaaataaaa atccccgctt tcattatgga
     1381 ttttattttt gctaatcgtt ggatcatcgt cagttccttt ttactcgttt atattttcct
     1441 tggctatatt gggattcgac tgatttttgc tttaccggaa atgattctac gggatcgtcc
     1501 ttttcgagca gccattcggg aaagctggtc attgaccaaa tcgcgtcttt tggccatcac
     1561 gggccaattt attgtgattg gcggtacgat tttactcctt tcttctttag gatatatcgt
     1621 tgttattttg gcacaaagta tggttgaaca atttttccca gactatgcat tgatcagcgc
     1681 tgtgtttgct atgacgcttt tacaagggat tttattattc aatatcgtga tgtctaccgt
     1741 agggattttt tatatcattg ttgatttcat ggatgacgaa ggcttcttgc ctgagatccc
     1801 taagtggttt atcccacaag cccccaactt gcgtttctct gcgctgaaaa acactggttt
     1861 gactcttttt gcagtttttt tcggtattgg cgtctgtctt tacaatatgg attatctgac
     1921 ttcagctgtt cagacaaaac cagtgactgt ctctcacaga ggtgtgagta cccaaaatgg
     1981 ggcgcaaaat actttagaag cacttgaaaa aacaagcaga gactatcatc ctgattatgt
     2041 agagatggat gttcaggaaa caaaagatgg tcatttcgta gtgatgcatg atgcaaatct
     2101 tcgtcactta acaggggtaa atggaacacc acaggatctt acattgaaag aattgacaaa
     2161 tcttacagtt actgaaaacg gagtgcaagc ccctgtttgt tcttttgatt cttatttgga
     2221 aaaagcacat aagctaaacc aaaaattact tattgagatc aagacctcta aaaaagattc
     2281 ggatgacctt gtcgaacgat ttgtccataa ataccgcaaa gttatcttag agaaccatca
     2341 tattttgcaa agtttgacgt accaaaccgt ttcagattta aaagaagcag aaccaaactt
     2401 ttatgtgggc tacatcttac ctttcaatat tgttgggccc cctgtgacgc ctgctgattt
     2461 tctgacaatg gaatatagta cgatcaaccg taacttcatt aattctgccc accaagacgg
     2521 aaaagaagta tatgtatgga caatcaacga ttcagatgct atgagtcgca tgatgtttta
     2581 tggtgtggat ggtatcataa ccgatgagat gggactacta aatgaaagtg tgaaacaaac
     2641 agatgaagaa atcacttatt cagataaact gctaaatttt gttttgggag tcggctaatc
     2701 aaaaatcgtt aatgaaaaaa gtctttatac aacaaaaaac gcataacctg ccaccgcagg
     2761 tatatgcgtt ttttgtttga cgctcataat gtgtgcttaa ctttctcttg attccttcat
     2821 cgcacgaaca atagctaaaa tagcaggaat tgcagagatc actgcaagaa taattcccat
     2881 tttccctgaa ctttttcttt ttggcatatc ttctcgctcc cttctgtctt tatttatatt
     2941 ataagggagt tcttgcgaat tctcaaaaat actgtttaag aaaccaattc agcaggtgcg
     3001 ctaatatcac agaattttac gagcatgcga tacttgcttt cattgataag ttgatagtct
     3061 cgttggtcat aactttcaac atacacgaca gccgtgttga tatatgtacg ttcaatacag
     3121 ccgattagtt catgagatac tccgatcggt ttgcatatat aagatcttcc tacttctgct
     3181 tttaccattt cattccctct tttctttatt tttattttgt tttttcttta ttttataata
     3241 acaaattacc ttgtatttga aaacgttttt atccattttt ttatttttta ttttgagtat
     3301 ctttctgctt tttagataac tattttttaa cacgatctta gacgttgtga caaaaacgtt
     3361 tgtttatatt cctatgttgt tcgtgcgttt ttttctttat attgctaagt tatttataaa
     3421 gaggtgagca tatgatttat attcaagcaa tagtatttgt aaccttgatg attgggctac
     3481 ttatcggatt aaaagaaaat agctaaacca tttaagaaaa aataaataag gaaaatgata
     3541 aatgcttttt aattgcatca aaaaacgtac gatgctaaac ttggtataag ttcgaaagtt
     3601 taaaggaaac tttcaagaac taaattacat tacaaaggag atgaatttta tgacgtttac
     3661 aaaaacaaat atgcaatacc gcgtagcttt tgatacaact tctaaccgtt ttatggcgat
     3721 tgatgcacaa aacgaaaata acgttgcgtt tggtattaca atcgaacaag cagtccgagc
     3781 attaaacgaa tctagataag gagtgaacca ttccaaatgg cgactgatga gatctcggaa
     3841 aatcaataaa agacgtttgt aacggtaatc cgcgacaaac gtctttttgt attcattaca
     3901 tatatttgga aattgtctct ctttatctct agacctaaac aaaaacacaa gttctaatat
     3961 caaccatctc gttttttatg acaattttgt tagctgtttc gatgataata ttccgttatt
     4021 atctgtataa tatttaatat aatcttaatt gtcaaattaa gttagacaga tcatcaatac
     4081 ttacgtaact caaaaatact tccaaaggtg ttcgataatc cagtgatttt ctaggaatat
     4141 tatttcgttt agatgcggca gattgaataa aaaattcatc tactgaattg aaatccatag
     4201 atttcaataa accatctcta cgtaacaatc cgttagaatt ctcgtttagg cctctttgag
     4261 acggcgttcc tggatcagcg aaataaatgg caatatcatt gacattactg atctgtttcc
     4321 aatttgaaaa ttcctttcca caatcaaaag tgatggattt gaataggttt ttcggtacag
     4381 attcaaacca ttgatttaat tttttttcaa tatcaatcgc ttgtctacca cacggtttca
     4441 atgtgatgat aacttttgat aatcgttcaa ctaaggtaat tacagcactt ttatgtttca
     4501 gacctacgat agtgtcacct tcaaggtgac caaactcatt tgagaattgg ctataatcct
     4561 tttcacgttc atgaatagag cggcggaaag tttgttttcc acgtctttct tgatgtccat
     4621 tcggtttacg cttgcctttc atcggtaagt cagaagaatc aaatagcccc tttttgaaca
     4681 tacgataaat ggtacgagca gaacaactaa taggaaacgc tgcacgacca acaatcacat
     4741 cgggtgtcca tccttgaaca acctttcttt gaatatattc tgattgttcc tcgggtaaaa
     4801 caagcggacg tctaccacag tttgatttgt ttttcttata ctgttgataa tattctaagg
     4861 ctgattttcc ttgcttgaag aacagatata ctttatggat cgtttgtctt gaacgattca
     4921 agcaatgggc agttttagca acagattgat tcattttgaa ataagactct attattacaa
     4981 gttcatccgt tgtaagatgg gtataggtca tttgtactca ctccttataa ttctttggtc
     5041 ggaactattt tgagtgtagc a
//
LOCUS       DACDYI010000114         5020 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.116, whole genome shotgun sequence.
ACCESSION   DACDYI010000114 DACDYI010000000
VERSION     DACDYI010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.116"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            53..517
                     /locus_tag="IWM52_002745"
     CDS             53..517
                     /locus_tag="IWM52_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974923.1"
                     /translation="MELSLESWDRAVVELFPEGLMIDTNDGKIHLTAAELQKQMRYQI
                     DDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITMIHR
                     FIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            576..1016
                     /locus_tag="IWM52_002746"
     CDS             576..1016
                     /locus_tag="IWM52_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974924.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1028..1696
                     /locus_tag="IWM52_002747"
     CDS             1028..1696
                     /locus_tag="IWM52_002747"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP9974925.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1760..2383
                     /locus_tag="IWM52_002748"
     CDS             1760..2383
                     /locus_tag="IWM52_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP9974926.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2439..2825
                     /locus_tag="IWM52_002749"
     CDS             2439..2825
                     /locus_tag="IWM52_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAP9974927.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3038..4429
                     /locus_tag="IWM52_002750"
     CDS             3038..4429
                     /locus_tag="IWM52_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAP9974928.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1667 a          839 c         1056 g         1458 t
ORIGIN      
        1 aaggaacttt gagatattct ttaggatatt aaaattaact agcaccacgg gtatcgagct
       61 ttctcttgaa tcttgggatc gagcagtggt tgaactgttt ccagaaggtt taatgatcga
      121 tacaaacgat gggaagatcc atctgactgc agcagaactg caaaaacaaa tgcgttatca
      181 aatagatgac gaaaattctt tttctatttt tagacgtcag caaaagattc taaaaagctt
      241 tttaaaagtg atcagtaaaa aacggaatct gatcaaaacg acacagctgt tgaaaaaata
      301 tccaggtctg cttcacacgt ctattcaatt tcctcaattg attacgatga tccatcgttt
      361 tatacgtatg caacagctac catctaagaa gctgtttctt ccattgacgg acacatttcg
      421 agtagtcaac agagaagata aaaagaaagt gattgtgatt gactttacca gaaatcggaa
      481 tgtccttcac caagttgcaa tggaaaaggc caattaaggc tttttctttt tggatttttt
      541 tgtcataata aatattagat gatggaggag ataatatgat gcaaacatgg ttagaaaggc
      601 taatacgatt tcagcagaac tatcacaaac ttatgcaggg aagatatgcg aaatttgatc
      661 agttgaatcg cggtctattg atcgctgcgc ttgtctttag tttgctaagt cgttggttgc
      721 cctatagaat cggacaattg ttgttcatcg tctttttcgg atggatgata tttcgtttcc
      781 tatcaaaaaa aatctacccc agactaaatg agaatcaacg ttatttgaaa tatcttgata
      841 gagtcaaaga aaaatatcat cagataaaaa acaaaaagca agtcaaaatg gatcgaaaaa
      901 gttatacttt ttttgagtgc ccgaattgtc atcaaaagca gcgtgcacct aaaggaaaag
      961 gacgaatacg tgtgacatgc aaaacatgtg gtataaaatt cgaaacgaat gtttaaggga
     1021 gaaaagaatg aaattagtat tattacgtca tggcgaaagc gaagcaaatt ttgaaaatta
     1081 ctggacagga tggctagatg tcgccttaac agaaaaagga aaagaacaag cagcagttgc
     1141 cggtcaaaaa atgcttgaag ccggacttca attcgatgcc gtttatacct ctgttttgaa
     1201 acgtgcgatt cttaccggac aagtcgcact agaagaaatg ggtcagttat ggcttcctat
     1261 aataaaaaca tggcgtctaa atgaacggca ttatggggat cttgtgggta aaaacaaaga
     1321 taagatgaaa gaaatatacg gaaaagatca agtaaaaaca tggcgcagag gatatacgga
     1381 agttccgccg ttaaccgcag aaaatagatt tgaccgcaga tatgatcact tggatccgca
     1441 tttgatccca tacggagaaa gtctggaaat gacagtgaat cggatcattc ccttatggca
     1501 agatcatcta gcaccaaaac taaaagatcg gcaagattta ctagttgtgg gtcatggtaa
     1561 tagcatccga gcactgacaa aatatttaga agatgttcct gaagatcaaa tggatacgat
     1621 cgatatccca aatgcacaac caatccagta tacattagca gatgatttga caattctgga
     1681 caaaaaaata ctttaaagaa cacctttatc taaaagtgag agatagaaaa aagaagcaaa
     1741 ggaatggaac aaaaggaaca tgaaaaatta tatatgggac tttgatggta cattgtttga
     1801 tacgtatccc gcaatggtag atggtgcatg gcaagcacta aaagattttg gtatcagtat
     1861 ggacaaaaaa gaaatctact tcaaaatgaa aaagtattct actagttatt tgatcaatga
     1921 atcaaacttg aatgccagag agtttaatga attatttcac cgatatgaaa aagaaagtac
     1981 ggaagtttcc cgtccatttc cagaaacgaa acaagtactg gaaatgctaa aagacaacgg
     2041 cggtcgtcat ttcatcctga cccaccgttt gacagaatca acatggggcc tgttaaaaga
     2101 acatcgattg gcacatctga ttgaggaagt agtagggatc gatcaggact ttcctcgtaa
     2161 acctgatcca gcatctttaa attatttgat cgatactttt catttagaaa gaacagacac
     2221 gatgatgatc ggcgatcgtc gtttagatat cgaagcaggg aaaaatgcag gagtcgctac
     2281 ttgtctttac gacatcgatc attttctggg tgagatcccg gcagattatg tggtaggaaa
     2341 tctgaatgaa atccttacct tgcagagcat cattaaaaag tagtatcccc tttcaaagga
     2401 gatatgttat aattttcata aaggagtgat aaaattatat ggaaatcaga agacaaaaag
     2461 aagttgtaga agcatttcat tatgatatga gattgccgaa ccaagaagta gaaacagatt
     2521 tgaaagtagg cttctcgcct ttaaaagaaa cgatcgaaaa ctatccgaaa gaacatagca
     2581 tcgtaggagc acgcttagag ttccgtttag tgtttgatac atatgtttta tctggaagtg
     2641 tgagtcagat caatcatatc ctcaatcgga aaattgaagg tcaaaatgat gtcacacaag
     2701 aagaagtgga cgagctagta gcaccgttat tcgatatcgt acgccgaatg tcttatgagg
     2761 tgacagagat cgccttagat cagcctgggg taaaattgaa ttttcaatca gaaaaacaag
     2821 aatagacagt aaaaacatac ctgcgataga ttaaagctat cacaggtatg tttttttatt
     2881 actaattgta agcaaaaaga atgtcatttc gataaaaatc gtctaaaata taaaagtcac
     2941 tgaaaaaaat tttttcagga ggatggttga agaatcattc tcgggaacct ttacttcctt
     3001 cgatggactt gtcattaata atatgaggag gagaaaaatg acaacactca tcacagtttt
     3061 agtggttcta gtttttgcgg cattattata tgtgttctat cgtatgcaaa agcgccatac
     3121 aaaattttca acacgcgtct ttacagcatt aggagttggt atcgtctttg gcggtctgct
     3181 tcaattgatc tttggtgtag acgacaaagt cgttactcaa tcaatggaat ggatcgggat
     3241 tgtaggtcaa ggatatgtat cattcttgca aatgctagtt atgcctttag tgtttatttc
     3301 gatcattggt gcattcacaa aaatgaaagt ttctgaaaag ctagggaaaa ttagtgcgac
     3361 tgtattgact tctttacttg gaacaacagc tattgcagca ttgatcggaa ttggttcaac
     3421 aatgctgttc ggactccaag gagctaaatt cacacaagga gctgcagaaa cagcacgaat
     3481 ctctgaactg gcaacacgtc aggaaacagt tgaaaactta tcgattccac agcagatttt
     3541 atcctttatc cctaaaaacg tttttgctga tttagcaggt agtcgtgcaa caagtacgat
     3601 tggagtcgtt attttcgcgg catttgtcgg aatcgcttat ctaggcgtga aaagaaaagc
     3661 gccaaaagaa gcagcattct tttcagattt agtcgacagt ctgtataaaa tcattatgcg
     3721 gatcgtgacg cttgttttgc gtttgacacc ttatggagta tttgctttga tgacaaacgt
     3781 tttggcaaca agtgattttg cagctatcgt caatttaggt aaatttgtcg ttgcttctta
     3841 cgtagcattg cttgctgttt tgattgtcca ctcactgatt ttgattggtg caaaagtcaa
     3901 tccaatcaac tatttcaaaa aagcctttcc ggtattgagc tttgcgttta cttcacgttc
     3961 aagtgctgga gctttgccat tgaatatcga gactcagaca aaagctttag gagtagatga
     4021 tgcaacagct aatttctcag caagttttgg tttgtctatt ggacaaaacg gctgtgctgg
     4081 cgtttatcca gctatgttgg caacgatcgt cgcaccagca gctggagtga atgtctttag
     4141 tttggaattt attttgatgc ttgtggcaat tgtaacaatc agttcttttg gtgtagctgg
     4201 agtcggtggg ggcgcaacat ttgcttcact gatcgtttta ggggcaatga acttgccagt
     4261 agcaatcgtt gggttagtta tctctgttga accattaatc gatatggcac gtacagcagt
     4321 aaacgtcagc ggtagcatgg tagctggtat cgtgacaagt gcacgcatca aagatttaga
     4381 tcgcgatgtc atcaatgatg attctaaagt gatcgatgtg aatgcatagt caaaaagagg
     4441 ttgtgataga agtggtcagc tttaagaaat aagaagaagt ttcctaaaaa tagtttctca
     4501 aatttttgtg aaatttcggc ttatttccga gaagctactt ctgaaacacc gtgaataaga
     4561 ggttgtgaaa aagctgccct gcatcaagta ataagaacag ccaaacaaaa atagttcctc
     4621 atattttacg tttggttgag cttattaccg cagatgcaag cttttgaaca ccgtttaaga
     4681 aagaggtcgt gagcctgccg tttcactctg agtcacaaag agcaatttca aaaaatagct
     4741 tctcatgttt gttgaatatt gtgtcttgtg accgaggagt tgtcaggaga acaccgttta
     4801 ccagaggttg tgacttgttt ttagtcacga cctctttttt tatttattcc atataaatac
     4861 acattattta ttccgaaaag tgaaagcgca aataacaaaa acaaccaact tatttattat
     4921 taaagaaaag cgtatttaag taggacgatt ctgttgagag ctttcgtaga ttttcccatt
     4981 ttcctatggt aaactgacaa agttgagaat gttagaacag
//
LOCUS       DACDYI010000115         5010 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.117, whole genome shotgun sequence.
ACCESSION   DACDYI010000115 DACDYI010000000
VERSION     DACDYI010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.117"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(12..482)
                     /locus_tag="IWM52_002751"
     CDS             complement(12..482)
                     /locus_tag="IWM52_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAP9974929.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(641..1099)
                     /locus_tag="IWM52_002752"
     CDS             complement(641..1099)
                     /locus_tag="IWM52_002752"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAP9974930.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1270..2415)
                     /locus_tag="IWM52_002753"
     CDS             complement(1270..2415)
                     /locus_tag="IWM52_002753"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAP9974931.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2428..3345)
                     /locus_tag="IWM52_002754"
     CDS             complement(2428..3345)
                     /locus_tag="IWM52_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAP9974932.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3773..4336
                     /gene="efp"
                     /locus_tag="IWM52_002755"
     CDS             3773..4336
                     /gene="efp"
                     /locus_tag="IWM52_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAP9974933.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            complement(4476..>5010)
                     /locus_tag="IWM52_002756"
     CDS             complement(4476..>5010)
                     /locus_tag="IWM52_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9974934.1"
                     /translation="TFFEYLKERGLQGTELVISDAHKGLVSAIRKSFTNASWQRCQVH
                     FLRNIFTTIPKKNSKSFREAVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLD
                     DGFEDAFQYTVQGNSHNRLKSTNLIERLNQEVRRREKIIRIFPNCVSANRLIGAVLID
                     QHDEWLSSSRKYIKFAK"
BASE COUNT         1525 a          925 c          953 g         1607 t
ORIGIN      
        1 tttttattaa gttaaagaac ggattcaatt gcagcggtca ttttcaatgg agaagtttgt
       61 ggtgcaaatc gttcgattac tttgccattt ctatcaatca aaaatttagt aaaattccat
      121 tttattgctt tagtgacagt accaggagct tcatttttaa gataagtaaa aagaggatct
      181 gtattttctc catttacgtc aatcaattga tgcataggga aggtaacgcc ataagtggtt
      241 ttacaagctt ctgctgcttc agtcgctgaa tctagctctt gtttaaattg attggaagga
      301 aagcctaaga taaccagacc ttgtgtctga taagcttggt aaagttcttc taattgttta
      361 aattgaggtg ctagaccgca ttttgttgca gtgttaacaa tgattatcgg tttgcctgca
      421 tattttttca atgaatagac agtgccgtct tctaaagtag tttcaaaatt ataaatagac
      481 ataaagtttt ctcctttcta ttttatataa taaaaagagc gctaaactga aaactagcta
      541 tttgttttcc cgtttagaac tcttttcaga caataaatat ttaataaaat acgaagataa
      601 gatagctata ttgcttctca tctttacgtt cttttagaaa ctatgcttct acttcagacc
      661 attcatcgcg ttttgctaaa aattcacgag ccaaagcttg tgcaccttct agtgtatggc
      721 tggcagccca gccacattgt gtttcattgc ttgcaggtac ttcagtagct tcaagaacat
      781 ctttcattgt tgcttcaaga acatctaaaa tgtcttcgta gctttcatga ttcaatacag
      841 tcaagtaaaa gcctgtttgg catcccattg gagaccagtc tacgatgtaa tctgcgtgat
      901 tgcggattag ttcggcagtc aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt
      961 ctttatttgg ttgtttaaag cggacgtcgt atttaatgat gacatcgcca tgcgcacccg
     1021 tttttcgatc agctacacgt acataaggtg ctttgacttt tgtatggtca agattaaaac
     1081 tttcgacatt cattctcatt tgtaagactc ctatctgttt gttcattcgt tttgatgctt
     1141 ttcactaaag aattatttag tgaaatttac aaaaacgtct ttctacttga ttctaacact
     1201 tatcgtttcc aaggtgtagc aatctgaaat attttttaga tttttctgct aagtcgttta
     1261 ttgggacttc tagttcttga ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg
     1321 accatactca attcctacag aaagacggat caagccgtcc tttatgccag cctcttctcg
     1381 tttttctttt ggaatagacg catgagtcat gacagaagga atttccacta aactttctac
     1441 gccacctaaa ctttctgcta aagtaaatag ttggagtgat tcaacaaatg gaatagcaga
     1501 ttcttcattt tttaaagtaa aagaaatcat tccgctaaag ccgctcatct gctgcttagc
     1561 aactccatgg agttcatgat cgggtaatcc tggatagtag accttttcta ctgctggatg
     1621 agaaaataga aaatcagcta ccacaaaagc atttttttga tgttcctcca tacgaacact
     1681 tagtgtcttg attccgcgct gaagtaacca gctatcttgg ggaccaagta cggctccgat
     1741 agcattttgg taaaatccga tctgatcggc tagatcttta tggttagaag tcactaaacc
     1801 agctactaca tcgctgtgtc cgcctagata tttggtaccg ctatggacaa cgatatcaga
     1861 accgagatct aaaggtcttt gaaaataagg agtagcaaat gtattgtcag caataactat
     1921 caatccatgt tgatgagcca gagtggcact tttttctaaa tcagtaatct ttagaagcgg
     1981 attgctaggt gtttcaagat aaagtgcttt tgtattgggt ttgatggatt gttctatttt
     2041 atcaagatta ctcgtatcta tgatcgtgta ctcgagacca tttttagtaa gaactttgtc
     2101 aaatagacgg aatgttcctc catagacatc atctcctaat aagatatgat cgccagcttg
     2161 aaaaagagaa aaaacggcat gaatcccaga aagaccagaa gaaaaagcga atcctcgaac
     2221 accgccttcc aaatcagcaa tcagttcttc caatgcaaaa cgagtaggat taccagatct
     2281 tgaatattca tattgtttag gctggccgac gccatcttgc cgataagtgg atgtttgata
     2341 aataggtaca ctgacagctc ctgttgtggg atcttcactg atgcctccgt ggatcaattt
     2401 tgtttgaatg tgcatgtttc tttcctccta taagtaaatg ttttttgata aatagcggtc
     2461 tgctgcgtct ggaaaaatcg taacgacacg atgaccaggt ggtaaacgac gaatttctct
     2521 taatgctgct gcaaaagccg caccactcga acttccaact aatagtcctt gttctcgtgc
     2581 taactgccta gtatagttaa acccttctac atcagagatt gtctcgatct gattgattga
     2641 aaggggagaa agaaacggcg gaatgaattc tacaccgata ccctcaattt cgtgaggggc
     2701 tggatcacca ccatttaaca cagagccctc tggttctaca ccgattattc gaatgtcggg
     2761 atatttttct ttcaaaaagg tagaagttcc agcaaaggtt ccaccactcc caattcctgc
     2821 tacaaaacta tggatatttt ctttgatttc atgaaagatt tctggcccta acgtatgata
     2881 gtaagcagca ggattatctt tattttcaaa ttgaagaggc agatagctgt ttgatatctc
     2941 ctctgcaagt tttttacttt tgctgatggc accgatgatt ccttgctcac taggagaatg
     3001 gatgatcttt gctcccaatg ctttcatcaa ttgttgtttt tctaagctaa acttctctgg
     3061 aacaacaaaa accgttctta atttgtatgt gagtgcggcc agagcgatcc caattcctgt
     3121 atttccggca gttggttcga tgatcgtcgt tttttcattg atttttcctt gggcaaatcc
     3181 ttctttgatc aaataactgc ccaatctatc tttgatactt cctccaggat ttaaatattc
     3241 tagttttgca taaatagcag aacctttagg catagaatag tgctgattgt caaagcgata
     3301 aagcggtgtg gaaccaatag cttctgtaat tgtcgagata atcatgtggg cctcctaaaa
     3361 aaagataata aaaaaaggag ctgactatta aatcagctcc ttgaaagaca tagagaaaaa
     3421 ttgagtagat aaaaagataa gttacctggt actcattcta tgaagggcaa ttaatagaca
     3481 tgacaaatag tatgctgctt ttatgcacca tacaacagca acgattcaat tgtttttggt
     3541 tgctgactgt catgtgtatc cctccaagta aaatttttat taacgatagc atgaaaaaaa
     3601 ataaaagtca aaggcttgaa agaaaaaaat atttcgatag catcgtttac ccgtgtttat
     3661 ttttctgaca aataggcatg agaccttaaa agatagagaa tactctagtt ttctggccgg
     3721 ttgtctgata caataaagtt gattatgata aagaaaagaa ggagcaaaaa taatgatctc
     3781 agcaagtgat ttaagagccg gtatgacttt cgtacaagac ggcaaactga ttaaagttct
     3841 ggatgcaagc caccataaac ctggtaaagg aaatacagtt atgcgtatga aactgaaaga
     3901 tgtccgttca ggtgcaacat acgacacaac gttcagacca gaagaaaaat ttgaaaaagc
     3961 acatatcgac acaaaacctg tacaatattt gtacacgatg gatgatacag ctttcttcat
     4021 ggatttggaa acatatgagc aatatgaaat tcctgtagaa acagtagaag aagaaatgaa
     4081 atatatccta gaaaacatgg aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt
     4141 tcaattacca acaactgtcg tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc
     4201 aacagtcact ggttcaggta agccagcgac aatggaaact ggcttagtag tcaatgttcc
     4261 tgacttcatc gaaacagatg aattgttgga aatcaataca gcagaaggtt cttatgtaaa
     4321 acgtgcgtca aaataattcg ctaacggatt gataagaatt tgaaaacaga gcttgacctc
     4381 ttgaaaggca gatcatcgga tctgcctttc aagaggtttt ttgtagtcaa gtccacaatc
     4441 ctgtgtaaaa tactatacaa tgctctcact attttttact tggcaaattt aatatatttt
     4501 cttgatgaag aaagccactc atcgtgttga tcaataagga ctgctccgat taaacgattg
     4561 gctgaaacgc agtttggaaa gatacgaata attttttctc ttctgcgcac ttcttgattc
     4621 agtcgttcaa ttagattggt actctttagt cgattgtggg aatttccttg tacggtatat
     4681 tgaaaggcgt cttcgaatcc atcatccaat gatgcgcaag cttttgaata ttttggttga
     4741 tcgatataat catgaatcaa tcgattttta gcctcacgag ctaagttaat atctgtgaac
     4801 ttaaaaattc ctttaacagc ttctctgaaa gattttgaat tttttttagg aatggtggta
     4861 aagatatttc ttaggaagtg aacttggcat ctttgccaac ttgcgttggt gaaggatttt
     4921 ctaatggcag agactaatcc tttgtgcgca tcagaaataa cgagttctgt accttgtaaa
     4981 ccgcgttctt ttaggtattc aaaaaatgtt
//
LOCUS       DACDYI010000116         4949 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.118, whole genome shotgun sequence.
ACCESSION   DACDYI010000116 DACDYI010000000
VERSION     DACDYI010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.118"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..988
                     /locus_tag="IWM52_002757"
     CDS             <1..988
                     /locus_tag="IWM52_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974935.1"
                     /translation="KEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVEKINNFTKT
                     NQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTTTEVLMEKH
                     EQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQDLAEQKMAI
                     EKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQANLTPSPQ
                     RSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQEQARKEYEQ
                     QLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNEVK"
     gene            988..3600
                     /locus_tag="IWM52_002758"
     CDS             988..3600
                     /locus_tag="IWM52_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP9974936.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFCEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSLENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            3597..>4949
                     /locus_tag="IWM52_002759"
     CDS             3597..>4949
                     /locus_tag="IWM52_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974937.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PDQAAEMADNDKSGLSDLQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     G"
BASE COUNT         1798 a          772 c          983 g         1396 t
ORIGIN      
        1 aaaagaggaa ctaaataaag aagaacagcg agtgattgaa gaagaagtac aaaaaatcaa
       61 agagcggtac gaaaagaaac gcgacaatgc ttattccttg tgtgtagaaa agatcaacaa
      121 ttttactaag acgaatcaag aagcgttatc gaaagaaaaa gaaaacttgt taaaagaacg
      181 tattaaagaa cgaaaagaag agttctaccg cggtttacgt acagataagc taaagattag
      241 caaagaatta aatgaacaac tgaataagct atatcaaaca acgactgaag ttcttatgga
      301 aaaacacgaa cagatccagc aagaattaca agtacaaatt ccaaaatgga aaaaagagta
      361 tgaaattcaa caacaagaat tgaaagaaca agaagaaaaa caacggcaaa aagaaaaaga
      421 gcgtgaacaa ttagaaataa gaaaacggga acaggatctt gcggaacaaa aaatggcgat
      481 agaaaaacaa cggttagagc aagaaaagga aaaagaagca gctgctcgta aggaacgtca
      541 agaacaaatg gatacattgc gtcaagcaca gttgaattat ctagcacaac ctcaaccagt
      601 agctgctgtt caaacgccag tgaacaacca agcaaattta actccttcac cccagcgttc
      661 ctttaacgga tggatggtgg gatgtattgc aagtttaagc ttgcttctag gtggaggaac
      721 agtatttgct tttaatcata tcaatcaatc ttcacaggct gattctgtag ccacaacggc
      781 gtcaatcaag gaagaagccc aggaacaagc aagaaaagag tatgaacaac aattagaaga
      841 attattgaat cgtaccaatc agtcaacaac agagtcaagc atggaaaata gcaaagagag
      901 tgattctagt aaggctaaag aaggatcaag cgattcaact gtacaatcaa acacttccga
      961 aagttcagaa tcaaatgagg tgaaataatg cgtgttagag aaacaacgaa gcgaaaagac
     1021 aaacgattca atttagaaaa gaataggtat ctttctcatt tctatcgcta tactccctca
     1081 ggagaagagc taccagaaaa acatgacaaa cgcatttcga aaaagatttt ttatagtgga
     1141 cttgtatacc tccctttaga gtatttagta acaagagtac cagaagtttt caaccgattg
     1201 gaaattgtta agggaagctt acaaggaata aatctgtttc aattacagga aatgccacag
     1261 gtctttcatt ttgattcagc tggtttttgg aatccattta ctcctacaac tagtttgggt
     1321 acacgtttag caatttcgac agtttgtgtg gttccagctg tacttatggg attgaaaatg
     1381 gacatgtggt ttcgtcctct agcagatgga caaaaagggg ataatcgatt aatgacctta
     1441 aaagaaatcc aacagattta tccgcaaata cctgaaacaa aaactacttt tccaggttat
     1501 ggaggtattc caatcacaca ttacaaaaaa tattggtaca ttcaaacaga tactgttaat
     1561 acgtgtattg ttggtacctc tcgatcaggg aaaggtcaga ttgaagtgtt agccaccatt
     1621 gacaatttat ctcgtgcaga aaaacaaagt tcaatggtcg ttaatgatcc aaaaacagaa
     1681 ttatatgttg gtagtaaaga tgaactagaa gctagagggt atgatgtata tgcctttaat
     1741 atcaccgatc cactgcaatc aatgagtgat aatccattgg cactcatcgt gaaatactgg
     1801 aaacgaggag acatagatac tgcaacacag ttaaccaata ctttcacaaa tacgatttat
     1861 tatgatgcca acgcttcaga taacaaatac tttaacgaaa acgcgcagaa agctgtcaat
     1921 gcgcttattt ttgcgttgtt agaacaagct gatcaaacag gagattactc caaattgaca
     1981 ccgaataatc tcgttgagct tctttctgag attggcggtt ttaattatta tccagatcca
     2041 gacaatgaat tcaaacagtt gaatgcctta gatgaattta tgaatcagtt gccacctgga
     2101 aacattgcaa aaaaacaata tggggcaacg aaaattggat ctgataaagc gaagggaaat
     2161 attttatcaa cggcaatcac gggattgaat ccatttactc taacgaaaat tgcaaagatg
     2221 acttctcaat caacgattga ttataaatcg gttgggtttc caaaatactt agatttgaaa
     2281 ttacatgaaa gtcttttaaa tcaacgagtc gtagtagaat tttatcataa gggaaagaaa
     2341 tttcatgaag aacgtgtcaa agtaggctat aaaggatttt gtgaaatcaa ttttgattgt
     2401 catttagttg caggtgatca agtttgttta aaatacgatc atcaaggaaa agaatatgtg
     2461 gcttggtatg aattttctca aagagtattg aaagacgaag agggtaatat cgtttatcgg
     2521 aaaaaacgag gagaaaccca tttgccagaa ttagaaaaag aagccgttct aacattagat
     2581 gaggagaaaa gtaacttatt agtagagtct attcggatgc actataccga taaaccaatg
     2641 gctattttta tgcttacacc tgattatgat ccatctaacc atatcattgt tagtattttt
     2701 cttagtcagt tatataaaga attatctacg caatgtgtta agactaaagg agataaatgt
     2761 caccgcagga ttcattttat tttagatgaa tttgggaata tgccaccaat ggataatatg
     2821 gaaggaatca tgacggtcac agctggccga aacatgttgt gggatctgtt tattcaatct
     2881 tatcagcaac tttatgcgaa gtatggagaa caaaacgcag cgattatcaa agaaaattgt
     2941 cagacacata tttatattat gtcgacagat gataatacca tcgaagaatt ttctaaaaaa
     3001 gtggggaata agacggttga acaagagaac agtaccatca attcattagg tatgaatact
     3061 catatcaatc gcaatgttga ttcagatcgt atcttaactc ctgaaagaat ttctactttt
     3121 ttagaaggtg aaacgattgt actcgctcct ttaaaacgac aagatttgaa aggtcataaa
     3181 atacggccgt atcctatttt taatcatggg gaaacgaatc ttccttatgc ttatcaatat
     3241 attgaagatt tcaatccatc gtgggatatc aatgatatag acatacattg tacacatgcg
     3301 gacttggatc ttaaggcttt acaaattgat tatgattatt tcctaccaga tgaatatgct
     3361 agaaatctct atcatggact gcatgatcaa ttagaagaaa aaaacgaaga aaatatgttt
     3421 tataaaatcg ttcaatctct tgagaatgag acggctaaaa aattgttaat tcaattttac
     3481 gaagaacaag cagcagatcg acttcaagaa tatgttgaaa agttgatcaa aaataaagaa
     3541 atttctgaaa tgattgggaa acggttgtta ttggaatctg aaaaggtggt gaaagaatga
     3601 tcattggagg aattttttct ggcatagccg actggtggtc aggcttattc ggagaagggg
     3661 atgcaaaccc caaaaccgta acgaaagtct tacaagatta tggtaattat ctgcagtatc
     3721 aagatgtatt tagttggatt tggaacactt ttctgtgggg attgattaaa ggattatata
     3781 atctcaatac tttactagaa aaaaccattt atcaaagctt tgatttaaaa gacttattga
     3841 acgccgctgg ggtaaatgca ctatttcaaa gtttgattag taaggtattg gctctttttt
     3901 gtgtcgtcac attaatttat ttaggcttga aattttttat gagtaaacac ccaccaaaag
     3961 tcaaaaatat cttggttaat ttatttatgg ccatgatctt gatttttggt ggtagttctt
     4021 taatcgatga agggttaaat atcagtaaaa gcttttacgg agacgtcact gcagcaaata
     4081 aagcagataa aacgggatca cctgcttttc aaattattca aaataatgtg atcgatgtca
     4141 gtacgttact agcaactgac ccgaataaag tagaatccct accaacagat aaaagaaatg
     4201 ctttaacagc gaaaaatttt aaaacagcga atatcaatgg gattattact cctgatcaag
     4261 ccgctgagat ggcggataac gataaaagtg gattgtcaga tttacaaaag gatcggttaa
     4321 aggcattaca ataccgattg gaactagatg ataatgggaa agaaattcct gttcaaatta
     4381 gtgacgaagg cttagcaaaa tatgtctata cttcagggta tcgtcgatac agtagtaaac
     4441 caggtattat cttagctggt gaatcttctt tggcggtggc ctatttattt atcctattta
     4501 cgatcgttac ttgcattatt gagctggtat ttaaaaaatt ctatttagtg attgccgcgt
     4561 caacggactt tgagacaggc caacggatga aaagtgcgat agaagatatt tctcaatcct
     4621 tcttgttatt ggcgtttacg attttagaat tacgaatcta tacattaatg ctttcaggta
     4681 tcggtgattt gcatgcggca ggaaagatca atggattctt gtatgttgtt gctttaattg
     4741 tgctaactat tgcgctattc aaaggctctc aatcggttac gaagattttc ggtgttgata
     4801 cgagtctgaa aaacggcagt aattctcttc ttagcatgtt tgccttagga aatattgcga
     4861 aaaatgtggg tagcggagct aaaaaccttg caagtgctgg taagagtggt ttaagccatt
     4921 taaatgatat tcgtaaaaat ggatttggc
//
LOCUS       DACDYI010000117         4807 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.119, whole genome shotgun sequence.
ACCESSION   DACDYI010000117 DACDYI010000000
VERSION     DACDYI010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.119"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(89..1174)
                     /gene="xerS"
                     /locus_tag="IWM52_002760"
     CDS             complement(89..1174)
                     /gene="xerS"
                     /locus_tag="IWM52_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAP9974938.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            complement(3250..3768)
                     /locus_tag="IWM52_002761"
     CDS             complement(3250..3768)
                     /locus_tag="IWM52_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAP9974939.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1579 a          786 c          729 g         1713 t
ORIGIN      
        1 taaaaatgat aatgaagctt gcctttaaag ttgggctttt atctaatttt agagacaggc
       61 ttcattatta tactattttt tactacattt acaaagcgtt caaagcatca attgtagctg
      121 cattgtctat atgagtatat aaagttgttg tatttgtact tgtatgtccc aattgttggg
      181 cagttaatac caatgaattt gtctgttgat agagtctagt agccaaagta tgacgtaatt
      241 tatgtggact gacttggact ctgaatcctt ttgaatattt tgctaccatc cgttcgattg
      301 caataccgga tatacgcttg gcttctcctt gataaattgc tagaaataaa gctttttcgt
      361 tttctgttac tttgtatcgt tctttccgaa tagctagata attagctaaa tattccattg
      421 caaatgctgc tatatttact gcgtctctct tatttccttt tctagttact accacattat
      481 tcttttctaa agataggtcc tctaaattaa tatttacaac ctcggagagt cttaatccag
      541 atcctaaaat caatgcattg atcgccaggt ctctttctac atctcgttga taatagatca
      601 aagctcgttt gctcaaagtt tttttatatt cattggcaat ataatctaga taattttgtg
      661 tatcttcgtc tagaaataat tttttctcga ttgcgtttgc acgggcagct aacgtagttt
      721 tatctgtgat agttgctact tgatccatag gattattttc taagtatggt tttcctaaag
      781 gattattgga acttttcgaa agatatttaa atagaacctt taatgctgtg atatttctat
      841 tgatagtaga agtagataga cctaagttat tattatcttt gagcttagtt tttgttgtct
      901 ctttttttac attttccttt gtgcgacgtt tcaaatgaga tttgtacaag tttagatcat
      961 aggcagttaa ctgtcttatc gaggtgattg aagtatcggg aacgatagac tcactcagaa
     1021 gccattcata aaagagctta taatttttta gatattctaa agtagttcga ggtgattgat
     1081 ctttatcctc acgatcttga acaaaatctt ttatataatc gggcatatga agtatgattg
     1141 tatctatttt ttgatatatt tctgtatttt tcatgttatt catcctttcg ataagtaggg
     1201 gagaggagta tctctataat tggactatac caattataga gtgttttgtc aattggtata
     1261 ctctgaaatt tattaattta tttcaaaaaa gcagataaat aggtatagat ttaataattt
     1321 aaaataaatg gtatacatga ataggaaagg aaacagaagg aggcaatctt aattttttaa
     1381 attgtctaaa ttatatagaa agaacaatct ttctatataa ttagacataa gagagactac
     1441 ggttgatttg atattaacaa gcgtatatga atgtatatac aaataaaaaa ggggagggag
     1501 aagttaaaga aaattcagag aagtggggca ctcagtagta ttaacaattt aaaaggaatt
     1561 tcttaaagaa aatgggttgc atcctggaga tacaattttt ttagataaag aaaaaattca
     1621 agcaacaatt caaaaaaatc ttaactgctg agattgatgt agcaatatct caatcatcaa
     1681 caaagtacga tcctgccttt caaaagctta ttgataaatg tttattcatt ataaaaataa
     1741 aagaaaaatt tatgtcagct tttttcatca aagtggtaat gataattcgt ctattaattg
     1801 tattttttag gaaatatggt ataattgttt taacgaaaag gtaattcatg atacaccaaa
     1861 aaccactagc tggcgggcta gtggttttct tttacgttct attcagatta acgggtaggc
     1921 ttactgtctt ttttggcaat aattttgcaa tcaagccatt tacagatgta atgcgcggat
     1981 acttctgcta agattgcaaa aaacatatcg aaaaggtaat tcataataca acctcctccc
     2041 cttgccataa taggccgggg tagcagacag cataaatatt ataccataac tgaaaatttc
     2101 attttagtac atttcaatat attgatgaac cagcacaata aaacatgcca tgactaacag
     2161 aaaaaaactt attaaaccag atataacttg taaacatcca actatataga aaacgtaaat
     2221 tttctatata gttggattaa gataacgact atttatttta tcatattttt actataccct
     2281 atacttttac ttggtatatt tatgaataat cgcaaattta cccctatact actagtttgt
     2341 aagttagatt aataaaaata ctaaagttat ttgttctttt attaaataca cctacaactt
     2401 ataaattgat ggtatttaga taacacgaaa gaataaaaat aacagctgat agtaaagttt
     2461 gttgacgatt gctactcaat cgaaagtgag ttagtgcgta tgaacttgct tctgaaaact
     2521 tatgaaatga aagacctttt acatcttgca gaaatcgtta ctgggattaa actaagcaat
     2581 aaaaactaat gttcccctca ttctttgaac ttctactatt tgatttattt attctgataa
     2641 atgacgattt caccttcagg atcttcaaat cccgtataga atttatttaa gatctcttca
     2701 gtatcagtaa taggataggc gataggccac ataccctttg atggttagaa taaactgctc
     2761 aaaatctgta tcgtatgttg gctttagcat attaacaaag gctatctcca aattataaac
     2821 cataagcgtt gaatcgttct taacaatttc aattcttcct aacccaaagt cagaagatat
     2881 aattaccgga cttgccttac ccttcatgtg ggttgttgag attccatatt taaagggaat
     2941 tgtcggtact atcggcggtc tgaaaaacat cccaaaaata aaaaaatctt tcccctgcta
     3001 gtctcctaag tccctttgta gcggatatgg caccataatc tcattttatc gaagtgagtc
     3061 atggtttatc tgttggccaa ccaaaattga tacaatctaa cgatgcaccc tctacggcat
     3121 tctaggggag tgcacttgca ttcacccaat aaaaaccggc agttagcaaa gaacttatgt
     3181 agtcgtatgt tcttactgtt aacatacaag aggtctcctt tccacaattg cttagcagta
     3241 aacgggaatt tacaaagcta atctcttcca ttttcttatc tttgttctaa aagtcccaaa
     3301 tggagcaact gtatttacat gaataaattt atatacttcc catacagccg ttttggttgc
     3361 ctcgtctgcc cattttcttt tgtgtgcagt gaatagatct tcatcactca ttgaatcaat
     3421 catcatgtta atatcatcaa tattacgatt gagaatttcc tctaattctt tcaatggtag
     3481 atgtgcatag gtatttgtaa accacttata taactctcct aactgattcc atttaaattg
     3541 atcagaaggc gttttaacag ataatccatt tctttcatca ttttcccatt ttagaattaa
     3601 attcgtccaa ccaacttgat atgctaaatt ttcagcaggt gttctatcca cttcctcaac
     3661 tcttttatcc ttcaattctt caggaatagt ttcaaattct aaaatatatt tagtataagt
     3721 cctatttatt tcttttttta attcttcttt atttttatat gttctcatta tattacctcc
     3781 ataaattcct aattgtctcc tttaattcat ctataatttc atccacgata ttttaatccg
     3841 ttctacaatg catatctgat atgcattgac tgtctaactc atatgtgaat gatttataaa
     3901 atatcccaac gtctcatcta aagaaatgtt gatcgtcagc aaggagctaa aaaatacgaa
     3961 tatatttctt ttgtagtaaa atcggactgg tttgcatatc attttcttcg taaatatcaa
     4021 aatcatgtta actactatca attactaata aaaactacca ctttaagcgt taagaactgt
     4081 taagtttagt taagtgagga tagttcacaa aatccattgt taaacttcac gaaagtggta
     4141 aaactcaaaa tgctttatat aggattacag catcgctctt tcttcgattg cgcgttgggt
     4201 aaaaaatatt atgaagtaaa aattgacgat gacacgttcc ttacggctca tcagatgaaa
     4261 cagctacaaa aacgaaatga tcaactcgaa gaaaatttaa ttctaaaaaa gcgattgcca
     4321 tattcacgcc tcgctcgacg aactattaaa ggtcaagatt tacctgaaga tcatcctttt
     4381 taggaaattg aagcttttta tgttgcacaa tgcatttata atactacaat gatgttttca
     4441 ccagatattc ttctagggtg cggtgtaatg aaacagatac acttgcaaaa gaaaattcac
     4501 cgcgaattta ctaaaattat gaataattat gttaatcttc cgaaattttt ttccaaaatt
     4561 aagggatcaa gctggtgtta tagattgttt aaggttggca aaaaaataac ttctttttaa
     4621 acaattatag aaaaccgata ctctgttaag ttggttgaca cggtgtcgat ttttatgact
     4681 ttcttattct gaaacttcac taacggacag tacctaaaaa gtctatagta tggacgttta
     4741 cgagtactat tttatcaaaa atgctgctca tttcaactta tatgttttag tgaactaccc
     4801 ccactta
//
LOCUS       DACDYI010000118         4756 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.120, whole genome shotgun sequence.
ACCESSION   DACDYI010000118 DACDYI010000000
VERSION     DACDYI010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.120"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..391
                     /locus_tag="IWM52_002762"
     CDS             <1..391
                     /locus_tag="IWM52_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP9974940.1"
                     /translation="KNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSDKEGHFEI
                     TGLTEGEYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASVPMTGSNG
                     FQSYVLISCLLLGAGAISAVVYFKKKA"
     gene            404..1156
                     /locus_tag="IWM52_002763"
     CDS             404..1156
                     /locus_tag="IWM52_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9974941.1"
                     /translation="MIIRHPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTILSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLESNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            1172..1927
                     /locus_tag="IWM52_002764"
     CDS             1172..1927
                     /locus_tag="IWM52_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974942.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGHEKQEGVNG
                     AKFSVYDVSDILQKMDVKDLTTDQIESQLKNRVKKLSSDQLKLVSNGETKTIDQQTGV
                     FEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSEAS
                     QPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            1940..2200
                     /locus_tag="IWM52_002765"
     CDS             1940..2200
                     /locus_tag="IWM52_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974943.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            2197..4287
                     /locus_tag="IWM52_002766"
     CDS             2197..4287
                     /locus_tag="IWM52_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP9974944.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLSNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTISAVSLLL
                     LVLATFSYAVLRYI"
     gene            4314..>4756
                     /locus_tag="IWM52_002767"
     CDS             4314..>4756
                     /locus_tag="IWM52_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974945.1"
                     /translation="MKKLIIVVTSLIAMIVILVGCSNEKNKQTNQDNGVLKSGTMWKE
                     EVGGLVYNLKIIDETTWEYSESVWHPDPVQITVKRQKDYKGLERYKIVDSAGVREFIN
                     KSDSLFIVVPYEKNGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKY"
BASE COUNT         1761 a          760 c          928 g         1307 t
ORIGIN      
        1 taaaaacgca gaaggaaaat attatggtgg attagttgat acggataaag acggtgtcaa
       61 agaagcagta tgggtagacg atgtagcaaa tgcagcgatc ctaaaatcag ataaagaagg
      121 tcactttgaa atcactggtt taactgaagg tgaatattca ctggaagaaa caaaagcgcc
      181 tgaaaactat caaaaattaa cgaaagagat ctctttcaaa gtagacaaag attcatacaa
      241 agaagaaaac cgaatcacga tcaaaaacaa tcaaaaagct tccgtaccaa tgaccggtag
      301 caacggtttc caatcttacg tattgatctc ttgcttgttg cttggggcag gcgcaatttc
      361 tgcagttgtt tactttaaga agaaagccta gaaggatcag ctaatgatta tcagacaccc
      421 taagaaaaag cgaatcatgg gtaaatggat cattgctttc tggttacttt cagctgttgg
      481 tgtcttactt ttgatgcctg ctgaagcgag cgtggcgaaa taccaacaaa atcagcagat
      541 tgccgcaatc gatcgaacag gaacggcagc agaaacggat tcttctttgg atgttgctaa
      601 gattgagtta ggcgatcctg ttggtattct tacaattcta agtatttctt taaaactacc
      661 aatttatgac ggaacgtccg acaaaatttt agaaaatggt gtaggtatta cggaaggaac
      721 tggtgatatt acaggaggaa acggtaaaaa tcctttaatc gcaggtcata gtggacttta
      781 taaagataat ctttttgatg atttaccttc agtaaaaaag ggagaaaagt tttatatcaa
      841 agttgatggt gagcagcacg cttatcaaat cgatcggatt gaggaagtgc aaaaagacga
      901 actacaaaga aactttgtca cttacttaga atcaaatccc aatgaagatc gagtaacgct
      961 aatgacatgt acgcctaagg ggatcaacac acaccgtttt ctagtgtatg ggaagcgagt
     1021 aacctttaca aaatcagaat taaaagatga agaaaataag aaacaaaaat tatcttggaa
     1081 atggctttta ggaagtactg tttttctttc agtcatgatc attggttcgc tatttgttta
     1141 taaaaagaaa aaataaagga gggtaaccaa catgagaaat agaaaatggc tatttgtttg
     1201 tgcagccgct cttttttctt tcactgccta cacacagttg acgtttgcag acagacaaga
     1261 aagcacggat agcacagaac aaacaatcca aacgtcagat agcagcacga ttcaaagcag
     1321 cgatgaaacc gtcgattcgt caacagttga ttcttcagaa gaacctaaaa ttgctgaaga
     1381 cgatcaaata gtcaaacaga ccattcacgt tcaaaagatt atttcttcag aacaattaga
     1441 caaccagggg catgaaaaac aagaaggagt aaatggcgcg aagttttctg tttatgatgt
     1501 atcggacatt ttacaaaaga tggatgttaa ggatctcact actgaccaaa ttgaatccca
     1561 attaaaaaat cgagtgaaaa aactatccag tgatcaattg aagctagttt ctaatggaga
     1621 aacaaagaca attgatcaac aaacaggtgt ctttgaattc tcggtagaag tacaggcaaa
     1681 tcagaaacaa gcttattata ttgtcaatga atcaagtcct gaaaacattt ctaatagcga
     1741 ggatattttg ctattaacac ctgtttctga taaaaacggt gaattcttaa aggatgtgtg
     1801 gatctatcct aaatcagaag cttcgcaacc taaagaagaa gtaaaaaaaa tcgtttctac
     1861 tggcgtaaag aaaaacttct ttgagaattg ttgggatttt gttactcact tgttttctag
     1921 agattaagga agaaataaaa tgaaactgat caaattatta ttattcaatt tttcttgttt
     1981 aaccgcagtc tttttgtact gcggtttatt attttctaaa ctatctattt tgccaagcta
     2041 ccaactatac ctaatgagga ttttaaacat accagctgct tccatgccgc tagatattat
     2101 ggaaaagctc attaggaatc cattatttac tacgatcaat ttgatcgtta ttgtcggaat
     2161 ttcactagtc atttataaaa aagaggggat gaagggatga attttaaaca ctcgtggaca
     2221 agacggttaa aatatgtttt tcccataatg atgatgttag gtacattgtt tgggttaaaa
     2281 acagccaatg tctttgctga aaaagttatt gtcgatccgt caaatccaat ccagaacgta
     2341 aagaataatt ttattattcc aaaagatgtt cctaatgcaa aaacaaatat tactgtaaac
     2401 ggagcgtcaa aagtttacaa atatcatgtt gatgccaaca aaggtggttt tacagacgac
     2461 tacgtaggat tagtggaggg aaacaaaaat atacgctatc cttctttcca aaaagaatac
     2521 ggtgaaacaa accttgtttt agaaggcgta acaaccaaca ccaaagtcaa tattgactat
     2581 ggaaaaatcg gtacgtataa cgggaaaaaa gtcaatatca agctagttct atctaatatt
     2641 cacttatact cggatacgtt gccgtggaat ctactagata acaactatac aaaaactcat
     2701 tttcgagacg atggctataa aaatacgaat ggtgcaatga gtaaatctaa aaaaagaaca
     2761 gtactttgga tctcagacaa tcttttctca ggaattgttt accattcaac acaaatgaat
     2821 gtgcagctag tagccacata tgaggacggt agccccgttc aattttcagg agatacattt
     2881 atcagtttta actctttgaa ccctgcagga ggaaaaagca cggatcttaa aggagaatat
     2941 gcccactatg acaaaatgaa tacgacagat tggtatgtca gaaaagatac ggttctttca
     3001 gaatttaaat ctttctacaa caatctgaac gtggtaggtg gccacccagg aggaagctct
     3061 aagctgacac aagcggataa tgatttcaat aatttgcatg ataaattagg agatccgaag
     3121 tttggtcaag gtactgtgtc ctttaaaata tctgaagcaa atccgacgtt tgtgattggt
     3181 tcttctaatg ttcaaacctg gtttacttta tcttctgcga caatctttag tgttgttcct
     3241 gatcaacctg aaaaaacagg ggttgataaa aatggaaata acgtgaacga taaaatgttg
     3301 caagtaggag acacgattca atatcgaatc aaacagaagg tgaatcgatt aggagtggat
     3361 ttactagcgg tttacgatcg atttgagtta atcgataatt taccaaaaga agtaaattac
     3421 gtcgatgcac acgttgaaag tggtacaaat aaaaaattcg atgtatctgg tgaagtgacc
     3481 tatgacaaaa caaaacacca ggtaaaatat gcagcgaaag ctgatacatt aaaaaatcga
     3541 atgaagtata acggcgaaac ttacgaatta gtgatcaatg taaaagtcaa tgaattggcc
     3601 aatcaaaata gtgtagccaa aaatcaggga acgtcgatca ttaataaagt agaaaaggat
     3661 acaaatatca ttaccgttta tttccctaaa attccagtga aagaagtaca acaaaatgga
     3721 aaagatgtaa acggacgaaa tgacggtaag aagggtacgc cgacagctcc tttaaacgca
     3781 ggatctgaag tacaatatct agtcacacaa aaatggcaca caaaaggcgt agacgctgcg
     3841 tcagatcact acaaacaatt tagtattcaa gatccaattg aagctcgttt gacatataaa
     3901 gaaggttctg cgcaagtaat agataagagt aaaggaaaag atattacttc tgaaggcaca
     3961 ctaacctatg atagcaattc aagaacgctg aagtgggaag cttccgctga cttcttaagt
     4021 aacaatcttt tagatggacg agaaattcag ctgatattta cagctaaaac tccattacaa
     4081 tcagaaaaaa atattgataa ccaagccgta gtagcagtag ataatgtttc taataaaaca
     4141 aatgttgtga caattggggt tgatcctaac ttgccacagg tcattgttcc taaaacaggt
     4201 tctacacatt tagtaacgat ttcagcggtc agcttactat tacttgtgtt agcaactttt
     4261 agttatgcgg ttctgagata catttaaaat aaaaaaacga aagaatgaat aaaatgaaaa
     4321 agttaataat agtagttact tccttgattg ccatgattgt tattttagtc ggatgtagta
     4381 atgaaaaaaa taaacaaact aatcaagata atggcgtatt gaaatcagga actatgtgga
     4441 aagaagaagt aggtggtctt gtttataatt tgaaaattat agacgagact acctgggaat
     4501 actctgagag tgtatggcat ccagatcctg tacaaataac agtcaagagg caaaaagact
     4561 ataaaggttt agaaagatac aaaatagtag attcagctgg tgttagggaa tttataaaca
     4621 aatctgattc tttatttatt gttgttccct atgagaagaa tggcgtaaaa aaaataattt
     4681 ttcttggatc tagcaaggat gaaaagcgaa cgaaagagaa attgattcac gatggatcac
     4741 aatccaataa atataa
//
LOCUS       DACDYI010000119         4447 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.122, whole genome shotgun sequence.
ACCESSION   DACDYI010000119 DACDYI010000000
VERSION     DACDYI010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.122"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            156..1862
                     /locus_tag="IWM52_002768"
     CDS             156..1862
                     /locus_tag="IWM52_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAP9974946.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="IWM52_002769"
     CDS             complement(2020..2922)
                     /locus_tag="IWM52_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9974947.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3623)
                     /locus_tag="IWM52_002770"
     CDS             complement(2919..3623)
                     /locus_tag="IWM52_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAP9974948.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="IWM52_002771"
     CDS             complement(3654..4295)
                     /locus_tag="IWM52_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9974949.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
BASE COUNT         1377 a          948 c          768 g         1354 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagatt
//
LOCUS       DACDYI010000120         4377 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.123, whole genome shotgun sequence.
ACCESSION   DACDYI010000120 DACDYI010000000
VERSION     DACDYI010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.123"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            834..1757
                     /locus_tag="IWM52_002772"
     CDS             834..1757
                     /locus_tag="IWM52_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9974950.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            1773..2312
                     /locus_tag="IWM52_002773"
     CDS             1773..2312
                     /locus_tag="IWM52_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP9974951.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
     gene            3571..4005
                     /locus_tag="IWM52_002774"
     CDS             3571..4005
                     /locus_tag="IWM52_002774"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974952.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
BASE COUNT         1639 a          655 c          736 g         1347 t
ORIGIN      
        1 ttatgagcaa attaatgcaa aatttggcta gcaaaaaagc taaaaaatat atgctaataa
       61 tcgagcgaaa taaacacttt ttcaagtgag cgaatccgcc atgacaaagc tgaagcagtt
      121 tatttcagct ttgtcatggc gtgtgccgac ttttttaaaa gtctaggcac acgagcaaat
      181 atctttatca agctctaatt tgcgttttaa gacgtttgaa acgcctaagt atataaatat
      241 ataagaatag gtttaagagt gcttaaatcg aattttagag ggcatttaga caattttagg
      301 gttgttaggg cttgccctga ccgcctgtaa ggcgttttaa gaacaagata agggattttt
      361 atactaggac tgtcttaaaa tgtcttaaac gaacgctcag agcttctcaa agggttcctt
      421 atgaacggct tttagcaaag gagccggagg cgactgcgca gtataatgtg agagcagagc
      481 gatcacacct ttttagcgaa ccgtagggag caaggtttgg aattggcagt tttgataaat
      541 tcgcgaacct gtttagagaa aaaaggagtg attttttagg cgggaaaatt agcctcccct
      601 ctttttatat ctctttttat atctcttttt atatctcttt tatgtcggct gtgtccttac
      661 tctcccaagc aattcagagt tttttcaagt aggttttcta cgcttttcaa gtaggttttc
      721 tacgcttttc aagtaggttt tctacgcttt tcaagtaggt tttccgacat tattgagtat
      781 ttttataaag tatgttactt ttatttcata ctttataaaa gtaggagaag cttatggcaa
      841 atgaaattgt aaaatatgaa aatagattaa atcatattcc tctgagaaaa tttaatagtc
      901 gtgaaatgaa cttatttttt tcaattgctt cacgtgtacg tgaaaaaggt gtagatgaga
      961 ttattttttc atttcacgat ttgaaaaaac taagcggtta tacagaacgt ggggaacaat
     1021 ttataaaaga tcttagtagc acgtatgaca aattattaag tattaatgct tggaatgatg
     1081 atggaaatac tttaactaaa tttgttgctt tcacaaaata tagcattata cgagataaac
     1141 aacttgttat aatttcagtt aatccagaat ttaaaggact ttttaatcaa ttaaagaact
     1201 ggacaagatt taatctagag caatttacta atttggatag cacatattca aaaactatgt
     1261 ttcgattatt aaaacaatat cgaactcaag gctggactga attttccaaa gaaaaatttt
     1321 ttgatttact agatattcct aaaagttatt ggaatagtcc gtcaaatatt gacaaattta
     1381 tcttaaggcc aattaaagaa gaactcactc ccttatttaa aggattaact attcgtaaaa
     1441 aatatggtaa aggtcgaggt aaacctgtta tcggttatcg ttttacttgg aaagctgaaa
     1501 ttaaccatgc tgacgatttt tccaaaggga aacaagaaga tttacgtata aaattattta
     1561 atattgatca caatggagaa ttaacacgag aagaaaaatg gcgcgctaaa gatcgaatac
     1621 taaatcttcc cttaggcact catgaagctg attttaaaca acaacaacaa actgaagaaa
     1681 aacaggcaga aaaacgagaa ctttcaaaca aactcaaaca agatttacta ggaaatttac
     1741 aaaacttatt cgattaagct taaggagtat ttatggcaga taaaacaatc aaagaacttg
     1801 cggaagaatt aggagttagt aaaacagcaa ttaacaaaaa agtttctgac aaaaatcgaa
     1861 aactttggtt tgcaaaaaac ggaaacaagt ttgtgattaa cgaaaccggt caaaaagcta
     1921 taaaatcaat gtttttagtc gataacgcaa acttaaatcg aaaactagtt ggcgaaaaat
     1981 caaaaaccaa aaaccataaa aacaccaacc aagtttccga attaaaactc ctttctttcc
     2041 tagaaaaaca gttaaatcga aaagatatac agctacaaga aaaagataat caaataaaac
     2101 aaatgcaaaa attactcgac cagcaacaac aactcactct acaagcgaac caacaaattg
     2161 agaacctaca agaacagctc catttaactt atacagaaga aactccaaca accgaatcaa
     2221 ctgccttttc tgaaaaaaaa gcagacaata cacaaccact tattgaaaaa aagtggtggc
     2281 aaatttggaa atctaataga accaatctct aaaattttct agaagttagt tcggatagta
     2341 gaggaaaaag ttgtactacc tgatggggag atcactcaaa tttttatgag aaagaaaaaa
     2401 tagtttacaa ctgtaaacaa tcaatgttta tagttgtaaa ctatcaaact atatggtgac
     2461 ttattgctta aaataggttg aaatctcact caatttttgg tggaaaaaca aaataaattc
     2521 taatcgattt tgttgaaaaa gtcataaaaa aacaaatgtg attttaacaa aatttcaaaa
     2581 aaatcgtttg aaatttcgtt tatttttttt gagaattttg atcatcgtag ggtcattgta
     2641 gaatcattat gagatcatcg tgggatcatc gaataaagta tgggaaaggc ttgcggtgct
     2701 tggtattaag ggcattctcc gcctcccagg ttcttgacca ttttctctta taagtgggat
     2761 ttttaccacg ttgagagaaa atggtcaaga acctgggagg cggagaaaac ataccaagca
     2821 ccacaagcaa aaaaagttag agtaacttcc aaaaaagaaa aattttttag aacaactttt
     2881 gaaaatttaa ttttgattta tatccgttca atttaaacat ttgataaact gaaggctagt
     2941 atttaaaatg tgtatacaag tggtagcgtt aaaaaaagtg gaggtataac ctatgttaaa
     3001 aaaaactgtg ttggtactgg gttctatttc tctaggaata ttaggtttta gccccatgct
     3061 attatccgta attggaaaaa acaattccaa cagagaactt aaattgaatt caaattatta
     3121 caacagtcct aacgaagaat tgtttgtaaa agaaacggat aaataataaa aaagactgct
     3181 cttccgagca gccagttaaa aaataatgag tgtttattct tgaaaaagca acgcgcttat
     3241 aaagcgctac caaaaattta tcatatttta tgaaaaataa caatccagta aaaaatgact
     3301 ggtaaaaatt cacaaagaaa cggaaagaag caactgcaga aaatcaagcc ttaaaaagag
     3361 ctttttacct agaccaatta aaaatcacaa agtgagcccc tgtatcggtt gatacaaggg
     3421 ctacttggca tactcgacat tatgtctact aacctttaaa ataacacgtt ttataaaata
     3481 aaccctcttg acaatattta attttctgat tattctaaat atatacagaa agcgctttcc
     3541 taggatttta tatctaagga ggaagtaaca atgaaaaata aaattattaa aattattact
     3601 tgtctatttt tgttcggtat gattgttgga gcaagtactc cgttaaatct gtttcctttt
     3661 agtgctagag gtataaaagt ggaagctgca agttcacgat ataatcataa tcatcgtgga
     3721 tttacttgct ataaaatgaa ctattatgtt actaataaaa tgtgtagaga tttgaaaaaa
     3781 aattataata agctaaagac tcctgctcaa attgcatctt ttattcctat tggtggagtt
     3841 ggaacatggg ttattacaaa tgcttttagt agtgctgttg atgcaatgaa tgtctttgta
     3901 agagctgcaa atcaaggaaa aggtgtacaa ttaacatata atgctcactt tagcaataca
     3961 acatcatatc aatacaatga ttacgctcgt tatgtaataa aataatcaga aagtgaaact
     4021 atctcatgaa taaaaagatt ttagctgtaa ttatttcaat aatagtttta atcactatgt
     4081 tcttcatatt taggctaatt ttcaatataa gcttacaaaa aagtattctt tatctaattc
     4141 ctatagcatt tatcttagca atttttagaa gtatttacgg aaataaaaac tctaaatagc
     4201 taaaaataaa tcatactaaa ttttacaaaa agtggctagc attttctagc cactttttgt
     4261 attgtggagc gtgggttttg agggggattc aagggggagc ttgcgcccct ttgacaagcg
     4321 gttatttgca ccaaatacgg aggtatttgg ctagcaaata tagagcttgc caaaccc
//
LOCUS       DACDYI010000121         4346 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.124, whole genome shotgun sequence.
ACCESSION   DACDYI010000121 DACDYI010000000
VERSION     DACDYI010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.124"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(152..427)
                     /locus_tag="IWM52_002775"
     CDS             complement(152..427)
                     /locus_tag="IWM52_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAP9974953.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(399..1352)
                     /locus_tag="IWM52_002776"
     CDS             complement(399..1352)
                     /locus_tag="IWM52_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9974954.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            1964..3457
                     /locus_tag="IWM52_002777"
     CDS             1964..3457
                     /locus_tag="IWM52_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAP9974955.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            3592..3888
                     /locus_tag="IWM52_002778"
     CDS             3592..3888
                     /locus_tag="IWM52_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP9974956.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            3989..>4346
                     /locus_tag="IWM52_002779"
     CDS             3989..>4346
                     /locus_tag="IWM52_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974957.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILA"
BASE COUNT         1453 a          705 c          791 g         1397 t
ORIGIN      
        1 atttagtata gtaaaaacta gaaacaataa aatttgtgaa tgttgtatac ccccaaacaa
       61 tacacatatt ttgaaggatt aggattaata aagctaaaaa tatagtaagt atactaaata
      121 tatttactat atctagtata cttactatat tttaatcttt tgatcgtcta ttatatataa
      181 tccgttcctc gtcgtttaaa gtatcaacat aggcgtccat tgcaagtttg actatttcat
      241 acataggaat atcttttgca taactaagat cccgaatggt ttttagtacg ggaggatcta
      301 cttgaactgg ttttctttct ttcgcagtgt actttcgttc ccctaactcg acattaatat
      361 catctttaat tgaaatatct tcagcaccaa ttttcttctt atctttgttc gttggtaata
      421 atgccattta gtttaatctc ctttccaaga ggtaggatat gattggccat tgagtcagca
      481 tatttaggtg tataatcaaa tccttcaata tcaccatgtt gttcatagta ttcaattctt
      541 tcttctaatt ctgtaaagat gtctgcaaaa atagcccacc atcttcgatc tacataatca
      601 tttaattgga taccattctc accatacatt tctaaccgat ccgagccttt aacaatgctt
      661 ttaaaaatgt ttgattcacc atacttatca attgtttttt gatagtttgc gtgttgagag
      721 gaacgtcgca ctgagaaaag aacaggcaaa attccaatga tttgaagatg agactcttca
      781 aaattcgtga caatcggaac taaaacttta ttaataaaat cttctgttcc ttccattgcg
      841 taccgtttta attcttgcat tgcaataata taatctgcag cagttaagaa tgcacgaacc
      901 acattatctg ttgatggtgg gcagtcgata aagatataat cgaaattttt tctcaattct
      961 tctaaaggtt taatgaaggc caatgatctc ttacgacgat caggatattt ttcggacaag
     1021 tattcattaa gttcttcaga tccaggagag ccagccataa tatataaatg tggacttact
     1081 tgagtaatac ctgaaaccca gtcccctttt ttgattgctg cagacatgga tcgatcaaag
     1141 ctagtaattt gatacgttga tccaatgttg gaagtagtat ttctttgttg gtcataatca
     1201 attagcaaca cgtctttttc aaaaagttga ctagaaacaa tcgctaataa atcattgtct
     1261 gtagtcttac caatgccgcc ttttctacct gtaagcatta caacttttcc tagtttatct
     1321 gatgttttaa atgcagtccg taagttttcc atagttccct ccaatttcct atactaaata
     1381 tatttagtat acttcatata cttaatatat tcgatatatc taatatactc caaattgata
     1441 agaattcaga ctttataagg aatatagtgg atatactaaa tatatttgct atattcagta
     1501 tatcagatat attcaaaatg tcaaaatata tttgtgcgtt ttgcgttgac aatttagagt
     1561 gaaaagaggt aaacttaatt ctatagaaac cacagataat tcactattta caaagtagca
     1621 aaagcagtgg tactctttaa acagaaaaat tacataaaaa aaagagacgg aaataattga
     1681 ttttagcaga atcaattatt tctgaatcac atggttagga catgtgttca cgctcgtctc
     1741 aagaatatgt aagtagctac caactactta tattaaaagt tgatcggata ccttttccga
     1801 ttgcctttat tctaccaaaa aaaggcgagc aactcaacag aagaaagttt atttagtgta
     1861 cttaaattaa atattcttaa acactgaaga gagaacacat actatggctg agattaaagc
     1921 taaactgtgt tctctttttg tttgagaaag gacgttacaa tcgatgttga gcgaaattta
     1981 ttcagctatt cttaaaaacg gcttacgtac atacaaatat cgaaacagca ccatgaaacc
     2041 tgcaggtcat attgattcca gtaggagtgg tgctatcttt gcgtaccgta gcaagcaatt
     2101 aatgaatgtt gggcgtggta tggtcattac ttcggaagaa gctattttag agaacgaaaa
     2161 gaaactaacg cattggacac ctaataccta tcgctttggt acttatgcgg atgattatcg
     2221 aacagtggtt aaaggtcatt ctgagaaaaa cttaagtcaa atcaacacat ttgtagttga
     2281 tatcgattca aaagagaacc atcaaggcga aattatttta gcctgtttag atcaagtggg
     2341 ctacatgcca acgctaattt tagaatcaga tcatggttat caagtttatt ttgtactaaa
     2401 gacaccaggc tatgtcacaa agaaaagcaa tttcaaggtg atcgatgtag caaaaaaaat
     2461 ttcaaaaaca attcgtctac agttggctga aaaattgtcc ggagtggatc taggatgtaa
     2521 ccacttcgga attgctcgat tcccacaaga aaataatatt gtttttaaag aaatgaacta
     2581 tcagtattct tttgctgact ggattcaatg gtcgatgaag cttgaatcag atcaaaaaac
     2641 aaaagaagat cgagaacgaa aactaattgt ctttcctgag aaaaaagaaa ttcgtcaagt
     2701 agatgaaccg tggttcgata tgttacttca taaagcaaac atcgtcggcg gagaaggacg
     2761 tcttggtcga aataatgtga ttttcacgct ttctttagct tattactctt caggttatgc
     2821 attggatact tgcgaataca atatgttcac attcaacgaa cggttacagg aaccgctatc
     2881 tgaagcagaa ttagtaaaga ttgtacgtag tgcttattct ggtaattatc aagcagcaag
     2941 cagagaattc attattgaat tgtgccagca atgggtttca ccaaccttaa aaaataatga
     3001 tctgttcatt caacgacgtg gctggtggaa atttaaaaag ccacgagatc aaagaaaata
     3061 tagtcataaa aatgaatgga aagaagatct attggcttat ctaagcgaaa aaagtcttgc
     3121 tagtgagcct tatttgaacc tgtcaaaaaa agaattggct tcacaactaa cgatgcccaa
     3181 gcgaacactt gataagatct taaatgagtt acgagaggag aataaagtct tctacaaggt
     3241 aacacgagga agaaatggcg gaatgatcat tgcttcagtc aaaacgttgt tcgcgagggt
     3301 tattcaacta agtaaagaaa agagactaga ttattcaaca aggatcacag aggtatttgg
     3361 actcaacgag agcttagtag aagagacatt tagacaatta tcaaccccct tgaaccctct
     3421 taaagaagtg aatctttttg aagtagatac aggttgaaaa ataaaaggtg caaattgccc
     3481 tatacaatat atctatgata cttctgtagg tgttgctccc tttgcctagc tacgtgtagg
     3541 tggtatactt ataactgcgt atgcagtctt atgtatcatg gaggaagttg tatgtcgtta
     3601 aaaaattatg tctattcaca tcctgttaat gtgtttatca ttggtcgttt gggattatct
     3661 gtggaagtgt tctgtgagct ttatggtttt aagcagggga ctttgtcttc ttgggttacc
     3721 agggaaaaaa cagttgcttc tttgccgatt gagtttcttc atgggttgtc cttagcttct
     3781 ggggagacta tggatcaggt atatgattgt ttgtgtgtgt tagagcagga gtatattgag
     3841 tataaaatag ctaacgagtt aagaaaaagg aagaagtata tccagtagta gtttttattt
     3901 acttaaaaaa ttggttcatt gagcaaccta tcgctgctaa agtagcaaag gaaaacgtta
     3961 aaggaaagaa tttagggaag aaatcgaaat gaaatcggac gactggattg aacacacctg
     4021 gcttcctctg aaaagaaata gcaaacgaaa gaaattttgg gtattgcaat tatggccatt
     4081 aggaaaactt tgtagttacg gggcacgact tattcttgat ttaaccgttt actcgattgg
     4141 agtatttgga acggcttatt acttgataaa tgatcccaat aggattgcag catcagacat
     4201 ttttacagtg ctaatgacgt tgatttttgt catgctattg tctattttta ctcaattagt
     4261 catgcaattt accaggcaaa aattaacagc aggaaaactt ttcaaaagcc tattcgattt
     4321 aattgttgta aatgcaattc tagcga
//
LOCUS       DACDYI010000122         4316 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.125, whole genome shotgun sequence.
ACCESSION   DACDYI010000122 DACDYI010000000
VERSION     DACDYI010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.125"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(93..2540)
                     /locus_tag="IWM52_002780"
     CDS             complement(93..2540)
                     /locus_tag="IWM52_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP9974958.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            complement(2729..4165)
                     /locus_tag="IWM52_002781"
     CDS             complement(2729..4165)
                     /locus_tag="IWM52_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9974959.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
BASE COUNT         1107 a          994 c          741 g         1474 t
ORIGIN      
        1 aaaagatgga aaaaaaccat tttttctttt ggtatcatca tgccttgtct ttttattggt
       61 tattcgtttt tcaacccttc atcacacaag ttttacatgt cattatcgtc cggcaacgtt
      121 tctgatgttt cttcctgctc aatgaatact ttccgaggct tgcttccttc tgacgggcca
      181 acaactccat gagcttccag ttcatctact aaacgtgctg ctcggttgta accgatccga
      241 aatcttcttt ggagaaggga aatacttgcc gtttgcattt caacgacaag tgcttttgct
      301 tcttcaaaca gctcatcttg tggctgttct ggagcacctg tcgtttcaac ttcatctgtc
      361 ggcatcattt tctcttcata atgagcttct tgctgatctg taacaaattg aacgacccgt
      421 tctacttcgt ggtcagaaat gaatgctcct tgcacacgga tcggtttatt ctcacccatt
      481 ggcaagaata gcatatctcc tcgccctaag agcttttctg ccccattaga atcaatgatc
      541 gtccgagaat ctgtaccgct tgataccgca aatgccattc tggaaggtac atttgctttg
      601 atgattcctg tgataacatc cacacttgga cgttgagtag ctaggatcat atgaatcccc
      661 gctgcccgag ccatttgcgc taaacgaata atcgcatctt caacttcatt gctggctacc
      721 atcatcaaat cagctaattc atctacgatc actacgatga atggtaaggt tggatgtttc
      781 gttccgtttt ccaagttttt ctgttgaaca aattcattgt aaccagaaat atttcttact
      841 ccggttgccg cgaatttttc atatcgttct tccatttctt gtactacttt ttgtaaggct
      901 tgcgctgctt ttctagggtt cgtcacgacc ggcgtcaaca gatgaggtat gccgttgtac
      961 atattcaatt caaccatttt cggatcgatc atcatcagtt ttacttcatg gggcttagca
     1021 cgcattaata tacttgtgat aatcccatta attgctactg acttcccact accagtcgaa
     1081 ccagccacga gtaagtgagg catcttcgaa agatcagccg tttgtacccg tccagaaaca
     1141 tcacgtccta aaggtacttc tagtaattta tctgggtggt ttggttgtgc ttcgatgatt
     1201 tctctaaagg aaaccatact gatctttcca tttggtactt caataccaat cagtgattta
     1261 cctggaatcg gtgcttccat ccggacatct ttcgccgcta aagcaagcgc gatatcatct
     1321 gttaagttca caattttact tacttttacc cctacagccg gctggacttc gaattttgtg
     1381 acggatggtc ctaagctggc tttgacgacc ttggcatcga caccgaaact ttggaaagtt
     1441 tgttctaata cgccgatatt tttttcaatt tttttatact catcactttg atccacagtt
     1501 ggtatagaat caagcaaatc aactgtcggt aattcatagt cttgattttc ctgttctgct
     1561 tcaatctcaa attctaatga ggtaccgtca tcctctggca actcatctga aaaaccttct
     1621 gctgtattgg tctgttttgg tgtatctggt actggtttct ctagattcgc aggatggata
     1681 ttttcttgaa aactgtcgat tggaacaaaa cttagctgct ccggctctgt attttctggt
     1741 tcctgccatt catcagcagt attttccctt tgctggctca ccgctttttt cttctcatat
     1801 tctgcagctt ctttttctgc agcttctttt gcagctaagc gccttgcttc tttcgcttct
     1861 gcgcgctgtt tcattctctc ttcctttgcc gcttgctttc gagcttgctt ggcaggatca
     1921 ccttccaata attcctgtac tcgttctcca attgattgga taccatttaa cagctgatag
     1981 ccttccagat tactgaacag gaatgctcct cctgccagta atagcactgc tgctaaataa
     2041 cttccaggct gtgcaatcaa gaagtaagta aaatgataaa ggattgctcc aatcatccca
     2101 ccaccaacgt tattgtctac ttgactttgt tttatatcac tggctaacaa atcccatgtc
     2161 gtccccatga tatttggttc cccagtatgg agtttgccaa ataaatgggc atgtaacaat
     2221 aataagatac cgaaataaaa gagtatgccg ccaaaccagc gtcggttttt accaattgaa
     2281 aactctgtat tcttaatgac aatccaaaat cctaatacgg ctaataaaag acagaggatt
     2341 tggtaagtat ttcctgcaat gatccgaaag ccgtttgcaa tcaatattcc taaaaaacct
     2401 aattttaaaa agccgaagac agcaaataaa atcaaggcaa acccaacacc aatcgtcagc
     2461 atttgttcct gctgctgttt ttgctttttc gtttttttcg tctttttcct ttttttagta
     2521 ggccgtttct tttgtgccat ttattatccc tcatttcatc aagtttcatt atacaaaaaa
     2581 aagtaaggga actaaagttt tgtttgaaag aactgacgct ttctttcatt tttcagctta
     2641 ttttacagtt tttcttaatt tctaaatgat tttatcaaga aaaggcacgg ccatatccag
     2701 ccgtaccttc ttgttttaag tatgtgaact aaaggttctc tccattttct tcaatcagtt
     2761 tttggtacca gtaataactg tctttcttat atcgttccaa agatccttct tgtgtttcgt
     2821 cttgatcaac atatacaaat ccatatcgct tttgataacc gttcagccag ctaagcaaat
     2881 ctgtataaga ccaggtgcaa tagcctagga cttctgctcc atccgaaatt gcttctttga
     2941 tggctttgac gtggctttgt aagtaattga ttcgatagtc gtcatggatt tgtttatctt
     3001 ctgtcaactt gtcatactct cctaagccat tttccgtaat cagtaccggc atgcggtaac
     3061 ggcttgtgat acgacgcagt ccgatcctta atccttctgg gtcaatctcc cagtcccaat
     3121 ttgttcgttc gacaaatgga ttctcaactt ttttatacat tccaggaact ccagtttctt
     3181 ctgatgaacc tttttttcct gtcgtattca tttttcctgc accaactccg tttaatggat
     3241 tgaatgcatt cgtcgccgtt tgatagtaat tgatcccaag aaaatctggt ttagcagatt
     3301 ctagcaattc tgcatcgcca gcttcaatcg ttggtgccca tcctttttct tctaagaacc
     3361 tcatggcagc aatcggatat tttccccaca tatagacatc catccaataa ttggccatca
     3421 ggtcttctgc attttcagca gccagaacat tttcaggatt actgtcaatt ggataatttg
     3481 gcgtatacgc aaagcttggt ccgatttttc caggcatttc taactcgtga aatttattga
     3541 tgacagatgc atttgctaaa ttagcaatat ggttaacttc atacattcgt ttcgggtctg
     3601 tcacacctgg cggatgtgct gctaacaaat aacctaatga agtgaagata ttttgttcat
     3661 tcaaactaat ccagtaatgg actttcccgc ggaatgcctc aaataatgtt tctgcataat
     3721 gagtgaaatc ttcaatgatc tgacgtgatt cccaaccatt atattcatct tgcaatgctt
     3781 gtggaagatc ccaatgatat aaagtcaaca taggttcaat atcgttttca atcaattcgt
     3841 cgatcaatct ctcataaaaa gcgagcccag cttgattgac ttctcctcgt ccattaggaa
     3901 aaatccgtgt ccaagctaca gagaagcggt aagctttcag accttgttct ttcattaaag
     3961 caatatcttc tttataacga tgatagtggt ctactgccaa atcaccgttc gttcctttaa
     4021 atgtctttcc aggtatccgt acaaatcgat cccagactga ttctcctttc ccatcttctt
     4081 gccaagctcc ttctacttga tacgctgcag aagcagagcc ccacagaaaa tcttttggaa
     4141 atgaggtaag ttctttgtgt tccattcatc tcttcctttc ctataacctt caatcgttcc
     4201 tcttttagaa taacacgaaa ccccttccac aacaaaataa aattatgtaa aggaggttgt
     4261 gaaaaagtcg cttagtttcg agtagtaaga aaaattttgt gaaaatggct ttctac
//
LOCUS       DACDYI010000123         4212 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.126, whole genome shotgun sequence.
ACCESSION   DACDYI010000123 DACDYI010000000
VERSION     DACDYI010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.126"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            373..699
                     /locus_tag="IWM52_002782"
     CDS             373..699
                     /locus_tag="IWM52_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974960.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(816..1160)
                     /locus_tag="IWM52_002783"
     CDS             complement(816..1160)
                     /locus_tag="IWM52_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974961.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(1236..2483)
                     /locus_tag="IWM52_002784"
     CDS             complement(1236..2483)
                     /locus_tag="IWM52_002784"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAP9974962.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            complement(2480..3286)
                     /gene="proB"
                     /locus_tag="IWM52_002785"
     CDS             complement(2480..3286)
                     /gene="proB"
                     /locus_tag="IWM52_002785"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP9974963.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            3516..4043
                     /locus_tag="IWM52_002786"
     CDS             3516..4043
                     /locus_tag="IWM52_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAP9974964.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            4106..>4212
                     /locus_tag="IWM52_002787"
     CDS             4106..>4212
                     /locus_tag="IWM52_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974965.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMV"
BASE COUNT         1273 a          799 c          866 g         1274 t
ORIGIN      
        1 gtttaacaga ggttgtaaaa tcagcgtttt atcctaagta ttagagaaac aaaacggaaa
       61 aatagcttct catattttac cgttttttga gctaataccg agggtcagct gattgaacgc
      121 cgtttaccaa gaggttgtga gactgtcgtt taactctgaa tcacaaggaa caatttcaaa
      181 aaacagtttc tcatgtttgt tgaatattgt gacttatgat tgaggagttg gcagaagaac
      241 accatttatt cggaaaaagg ggatgtgaca cgatttttgt cacattccct ttttttctca
      301 aaataatgaa actggaaaat tttcagtagg taagctaaaa ttcatttaag aaaaagaatg
      361 gggggataat agatgctgga agaatatttt caggaagtcg ggttgacact cgttgaatta
      421 cccattgaat tttcagatga tcggttgatt tatcgtggct atatcaaaga attgccaata
      481 atcaatgcgg aaggtgaaac tagaaaagcg atgcagcaaa aaatagctgg tatgtatcag
      541 gtgtatcggg aacagctact ggctgaatca gcggaagaag aaaaagaaaa aacgatgaac
      601 ttgtccatgg atgagctgct tcgttattat gatggcgaaa catttgacgg attttcgctt
      661 cttaacgaag aggaaacaga tttaccaggc agctggtagg cgaacgccgt ttatatggaa
      721 aaagaggctg tgacaaaatc tttgtcacag cctctaaaaa gtccggcagt tttttgccga
      781 tcttcaaggc actctactta tcgtacggat attttttagc ggaaagtccg attcaagtgg
      841 tttttattca gctgatattt ataagataag tgaaaaatcc cagcaatcag tagcgtaatc
      901 gtataagcga ttgaatgatc gaaaaaagaa tagtaattga ttgtcgctaa taagcataat
      961 gcaaccatgt aactaatgtg caagcgtgta accagccctt tttgcccgat tttgtcaaaa
     1021 tactgcgacc atttttcccg ttgactccct atgcttcgtt ttttgaattg aaagaaaagg
     1081 gataaaatac cgactcccag aaaagcagcg atattcagag aaataaggta aataaaaaaa
     1141 atcattaaaa tttcaaacat attgacacct ttctagttgg atagaacctt tacagaaaaa
     1201 aggcaaaagg cgggaataat gtttcacacg aaacattatt cgcggatttg tccatcgcca
     1261 taaatgatat attttgtgga agttaattca tttaacccca ttggaccacg ggcatgcaac
     1321 ttttgagtag agataccaat ctctgcacca aaaccaaata ccgatccgtc tgtaaaacga
     1381 gtggaggcat tgacatagac agccgccgcg tccacttcat ttaagaaacg ttgactagcc
     1441 gcataactat ctgtcacgat cgtttctgaa tgtttagtat tatgccgatt gatatgtgca
     1501 attgcttcat ccaaagaatc gaccactttg acagcaagga tataatctag atattcagta
     1561 tcccaatctg tgtcttctgc taaagcagcc gtctctagat attctaacgc acgagcatcc
     1621 gcacgtaaag agaccccgtg ttctaccagt tctttttcaa tagctggcaa gaaaaaaggt
     1681 gcgatctcag aatggatcag caatgtttct gccgcgttac agacagatgg acgggaagct
     1741 tttgcattga cgatgatgtt cactgccata gataactgtg cttctttatc gatatatacg
     1801 tgattatttc ctgtacctgt ttcaatgata ggtaccgttg cctgttcttt gactcgttgg
     1861 attaattttg ctccgcctcg aggaatcagc acatctaagt attctgtcaa tcgcatcaac
     1921 tgtcctgcag tttcatgaga agtatcatcg acaaactgga tggcataagg agattcgcca
     1981 gcactgacca gtacttgctg caataattgg actaataatt gattcgagaa gaatgcttct
     2041 ttgccaccgc gaaggataac ggcattccct gatttgaatg ctagactagc tgcatctgtc
     2101 gtgacatttg gacgagattc gtaaatgatg ccgatcacgc ctagtggcac gcgctttttg
     2161 ccgattctca atccgtcggt gttcgtccac atctcatcta cttttccaat aggatcgtct
     2221 aacgaagcaa ttttttcgat ctctgtagcc atggcattga ttcgctcagg tgtcagctgc
     2281 aagcggtctt gcatcgtttc actgatattg ttttcgactg ctttttctaa gtctttatga
     2341 tttgcttcaa tgatcgcttg ttgattcttt ttgatggttt gtgccatttg aacaagtagc
     2401 tcatttttgc ggtttgtagg cataagtgct agttgttgcg cgctatcttt tgcctgttgt
     2461 cctaattgca tcaagtcagt catttcaagt cctccttaaa taaagtacct acttcttttc
     2521 ctgcaagaat atcaaaaata attgccggat tttttccatt tgctaaaatc attgcgctgt
     2581 ttgcttcaaa tatcctagaa gcggctttta atttactctg cataccgcct gttccaaatg
     2641 tgctaccagc accacctgct tgtgccagaa tcttttcatc gattttatcg atatagcgat
     2701 acatctgagc ctcgggattt gtagtcggat tatctgaata aaacccgtcg atatcagaaa
     2761 ggacgatcaa aaggtctgcc gcgacaagtt ttgccatgat ggctgacagc tgatcattat
     2821 cgccaaattt tgtcagatga tctaattcat ccactgctac agtatcgttt tcgttcacga
     2881 tagggatcgt gtccataatc aaaagctgtt ccaatgtatt cctcacattt ttacgacttt
     2941 caggataatc taccacgtct cttgtcacga ggatctgtgc cgtctgttga ttatatacac
     3001 tgaatctttg attatagata ttcatcagtt cggcttgacc aacagccgcc gcagcttgct
     3061 gttcaggaat caaagtcgga cgttctgaca aatgtaattt atgcatacca acgcctacag
     3121 ctcctgatga gactaagatc acttcctttc cccgatttct aagatcagaa aggacaaaag
     3181 ctaactgatc gattgccgca agattgatat ttccattggg ataaatcaat gtacttgttc
     3241 ctactttgac aataattcgt tttgcatttt taatttcttt acgcatgtga cacctcaatc
     3301 aaatatccac tcattaattt cgttctattc tacttgtttt gacgagtaaa gtcatgtaaa
     3361 aaatagtttt tttgtacaac ttgcagtcgt ttggaaaaag aacgaattaa tggtttactt
     3421 taagatagca gaatttcttg aagaatttgt aaagaaagtc ttttacagcc tcggatatgt
     3481 tagaatagga gtagtttatt tagggaggaa aaccggtgag aaaaagagga tttgaagtta
     3541 tttctaaata taaagatcaa aatatcaatc taccgcagcg cgctacacat catgctgctg
     3601 gctatgattt tgaagcagca gaagaggtcg tgattccaag tatctggaaa gcaggctgga
     3661 aaaaggggtt ggaaacagtc gcagatggat tgaaaaaaga tgtatcagaa aatgaaaaac
     3721 aattgaaacc aaccctcgtg cctactggaa tcaaagctta catgggagaa gatgaatttt
     3781 tgcagcttgc taaccgttca agcaatccgt tgaagcggtt tttagtgttg acaaacggag
     3841 taggtgtgat tgacagtgat tattacaata atgaggcaaa tgaaggccat atcatgttcc
     3901 aattcaccaa ctttggtttc aaggatgtag tgatcaaaaa aggcgagcgt atcggccaag
     3961 ggatcttttt gccgttttta aaagcagatc aagatcaaac gactgctcaa cgtcaaggcg
     4021 gatttggttc ttccagtaaa taaaacgtta aagatactta agcttgattt aatgtctcat
     4081 gattaggata aaaggaggcg gtacaatggc aaaaaaatca aaagtacaat ttgtttgcca
     4141 aaattgcggg tatgtgtcgc ctaagttctt gggacgctgc ccaaattgcg gaaaatggaa
     4201 tacgatggtc ga
//
LOCUS       DACDYI010000124         4073 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.128, whole genome shotgun sequence.
ACCESSION   DACDYI010000124 DACDYI010000000
VERSION     DACDYI010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.128"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            591..1280
                     /locus_tag="IWM52_002788"
     CDS             591..1280
                     /locus_tag="IWM52_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9974966.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="IWM52_002789"
     CDS             1267..2475
                     /locus_tag="IWM52_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9974967.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="IWM52_002790"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="IWM52_002790"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAP9974968.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
BASE COUNT         1320 a          703 c          812 g         1238 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gta
//
LOCUS       DACDYI010000125         3956 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.129, whole genome shotgun sequence.
ACCESSION   DACDYI010000125 DACDYI010000000
VERSION     DACDYI010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.129"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..156
                     /locus_tag="IWM52_002791"
     CDS             <1..156
                     /locus_tag="IWM52_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HAP9974969.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..1452
                     /locus_tag="IWM52_002792"
     CDS             175..1452
                     /locus_tag="IWM52_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9974970.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1462..2118
                     /locus_tag="IWM52_002793"
     CDS             1462..2118
                     /locus_tag="IWM52_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9974971.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2118..3494
                     /locus_tag="IWM52_002794"
     CDS             2118..3494
                     /locus_tag="IWM52_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9974972.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3624..>3956
                     /locus_tag="IWM52_002795"
     CDS             3624..>3956
                     /locus_tag="IWM52_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9974973.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT         1594 a          424 c          693 g         1245 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      361 aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat tgaagaaaaa
      421 ataattcaat atgatggatt agcaaaatta aaagatgcta aagtagttag cggagaacaa
      481 agaataaata gagaagattt atctgacgaa tttaagaatg ttgtttcact cgaagctaca
      541 aataatacta aaagaaatat tttatttagt agtggagtat ttacaattaa agaaggtaaa
      601 aatatagaag aaaatgataa gaattcaatt attgttcatg aagaatttgc taaacaaaac
      661 aatctaaaat taggtgatga agttgatctt gaattactag atattgaaaa aagtggaaaa
      721 ataaaaagtc ataaatttaa aattataggg atcttttctg gtaaaaaaca ggaaacatat
      781 acaggattat cttctgattt tagtgaaaat atggtgtttg tagattattc aacaagccaa
      841 gagatattaa ataattcaga aaataatgaa attgcaaata aaattttaat gtattctggt
      901 agtgcagaat ctacagactt agccttaaac aaattgaaag agcttaaaat tgatgagtca
      961 aagtattttg ttgaaaaaga ttctaatgcg tttgaagagt ctttagagtc agtgagtgga
     1021 ataaaatata taataaaagt aatgacttat tctattatgt taggtgggat ggttgttctt
     1081 tcattaatct tgattctatg gttaagggaa agaatttatg aaataggtat attcttatct
     1141 attggaacat ctaagataca aattataatg caatttatat tcgagttaat attcatatcg
     1201 ataccaagta taatatcttc cctattttta ggaaatgtat tactaaaagt aattgtagat
     1261 ggattcatta actcagagga ttcaatgatt tccggtggaa gtttaataaa taatagtagt
     1321 tttatgtcaa atataacaac acttggacaa agttatttaa tattaataag tattattgtt
     1381 ttatcagttg tatttgcttc ttcattaatg ttaatcaaga agcctaaaga aatcttatca
     1441 aaaataagtt aggagaaaat aatggatata ttagaaataa agaatgtagc atatagttat
     1501 gcaaattcta aagaaaaagt tttgtcagga gtaaatcaaa aatttgaact tgggaagttt
     1561 tatgcgatag taggaaagtc aggaacagga aaatctacac ttctttcctt acttgctgga
     1621 cttgataaac ctcaaacagg aaaaatattg tttaagaatg aagatataca aaataaagga
     1681 tatagtaatc atagaaaaaa taatatatct ttagtatttc aaaactataa tttaatagat
     1741 tatttatcac caattgaaaa tattagatta gtaaataaat cagcagatga aagtatcttg
     1801 tttgaactag gtttagataa aaaacaaata aaaagaaatg ttatgaaatt atctggtgga
     1861 cagcaacaaa gagtagctat tgctagagca ttggtatcag atgctccaat aatactagct
     1921 gatgagccta ctggtaatct agacagtgtt actgctggag aaataattaa tatattaaag
     1981 acattagcta aagatagaaa taaatgtgta atagttgtaa cacatagtaa ggaagtagca
     2041 gattctgcgg atatcatttt agaactaagt ggtaaaaagt tgaaaaaagt aaataaaatg
     2101 aatttggagg ttgaataatg ataaaaaatg catttgctta tgtaactaga aaaagtttaa
     2161 aatcattaat tatcatatta gttattttat ctatgtcaac tttaagtctc attagtttat
     2221 ctattaaaga tgctacggac agagcttcaa aagaaacatt tgctaatata acaaatagtt
     2281 tttctatgga gataaataga caagtaaatc caggaacacc tagaggtggg ggaaatgtaa
     2341 aaggtgaaga tattaaaaag atatctcaaa cagatagtat agactcctat gtaaaaagaa
     2401 taaacagtgt tgcagattta gttgattatg atattattga aactcaagag actcttgcga
     2461 atcaatcacc tgaaagagca aagaatttta aaagaacagt tatgttaact ggggttaacg
     2521 actcaacaaa agaaacaaaa tttgtatcag aagcatataa attagtagaa ggaaagcatt
     2581 tagaaaatga agataaaaat aaaatcttaa tgcataaaga tttagctgaa aaaaataacc
     2641 ttaaagttgg agataagata aaaataaaat ctaatttatt tgatgctgat aatgagaaag
     2701 gtgcagatga aacagtagaa gtagaaatta aaggtctatt cgatgggcat aatagcggtg
     2761 tagtaagtgc agcacaagaa ctatacgaaa atacattaat cactgatatt catacagctg
     2821 ctaaagttta tggtaatacc gaagatacag ctgtatacca agatgcaaca ttctttgtaa
     2881 aaggtgataa gaatcttgat agtgtaataa aagatcttgg gaaattagat ataaattgga
     2941 gagaatataa tttgattaaa agttcatcaa attaccctgc attacaacaa tctatatcag
     3001 gtatttactc aatttcaaat aaattatttt ttggctcatt aatatttgca ggagttgtag
     3061 tttcattatt attattctta tggatgaatg ctagaaagaa agaaatagca gtattactat
     3121 cattaggtat atcaaaatta gaaatttttg gtcaattcct aattgagatg gtatttatat
     3181 caatccccgc attcgttggt tcctatttct tagctcaata tacagctgac aagcttggga
     3241 ataatatatt gaataaggtt actggtgata tagctaaaca aatagctaaa caatctgcat
     3301 ctagccaatt aggtggtggg gcagaagctg aaggatttaa caagacatta tcaagtttag
     3361 atatcaatgt attacctaaa ttcatgattt atgtagttat atttatgagt ttagtacttc
     3421 ttgtatcatt gattatttct tcattcaata tattaagaag gaatccaaaa gaattattaa
     3481 ttgacaataa ttaaaatttt cggtgctttt tagcaccaaa aatgagagtg taatataaac
     3541 cgtgtaagta accatcaatc ctaggcgggt ggttgcttca cggtttttat ttttctaaaa
     3601 taagatcaag aggtgattac acgatggcta gaaagaaaag aaatcctgat gctgagaagt
     3661 tagctgaatc cattttgaat acttatcaac ccgaatctgt agaggatatg caagatgttt
     3721 taaaagatgt gtttggtccc ttatttgaaa aaatgcttca aggagaattg aataaccatt
     3781 tagggtatga cgcccattct aaagagccaa agaaacatga caatcgacga aacggttatg
     3841 gcaataagac gcttaaaacg agctttggta aagtaaacat tgatgttccc agagaccggg
     3901 aggcttcttt tgaacctgag ttgattccta atagaaaacg agatgtctcc gacatc
//
LOCUS       DACDYI010000126         3915 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.130, whole genome shotgun sequence.
ACCESSION   DACDYI010000126 DACDYI010000000
VERSION     DACDYI010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.130"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(25..1530)
                     /locus_tag="IWM52_002796"
     CDS             complement(25..1530)
                     /locus_tag="IWM52_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9974974.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1527..2213)
                     /locus_tag="IWM52_002797"
     CDS             complement(1527..2213)
                     /locus_tag="IWM52_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9974975.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2406..3827)
                     /gene="gndA"
                     /locus_tag="IWM52_002798"
     CDS             complement(2406..3827)
                     /gene="gndA"
                     /locus_tag="IWM52_002798"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAP9974976.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1082 a          837 c          676 g         1320 t
ORIGIN      
        1 aaaaaaacaa gtaaagcaca tgctttactt gtcttctgcc tttgcgatag gaataaagat
       61 tctaaaaatt gtcccttgcc ctactacact ttctgctaag atacgtcctt tatagttttc
      121 aaccagttgt ttagcgatag acaaccccag accatttcca cccttcgttc tagctcttgc
      181 tttatccact cgataaaaac gattgaagat cttattaagg tcttctttgg gtattccttc
      241 gccaaagtcc tgaatcgcga tctcaaattc attcagagtg gaagaaatag agatatggac
      301 ttcttttcgt gtagttgaat actttactgc gttatccaat atgataatga ttaattgttc
      361 aaagtgatta cgataaattt gcagggtcac ttcttttgtc aaatcatcat ctaaagtgat
      421 cacatagtct ggatataata tcttgaaatt attataaact tgataggtga cttcttttcc
      481 tttagttgtt tcattcgcat aatgaacatc tacttgttct gctcttgata agtctagcat
      541 ctcttgaact aagcttttca tacggctgat ttcttgcatg ctggcgctta atgattcttc
      601 taatatctca ggatcatctt tcccccaacg atttagcaga ctaagatgcc cttcaataat
      661 tgcaacaggt gtccttaact catgagatac gtcttccacg aattgctctt gttgttcaat
      721 atacaaacgc atacggtcaa gcatctcatt gaatatttca gctaaatctg ccaattcatc
      781 atttgtgtcg atttcaggca tgtggatatc tgattgcgga tctttacgta tagtatccat
      841 tgtatctctg agtaccttga gtggttttaa gaaataagaa gataaaaaga aacctaaaat
      901 actacttaag atcaaagaaa caacttctaa aacaataaga gtcaataata gcttatttcg
      961 aatatcatag aaagaagata attcatagaa gctttgcgca tagccgatct tttctcttgt
     1021 acctttagaa tatataggct ggatagataa aaagccagtt tttccttcta gtgtaacgat
     1081 cgttggaact ttacgtgtag tctgaatcag gtttcgactg ttttcatggg ttttaaaaat
     1141 caagttctcg tccaaatcat aaacataaag atccattgat ggctgcccta gctcagctat
     1201 aaaggaatcg atcttcatta agtttccttc gaccagtgaa gtatgatcta cgacctcatc
     1261 tccgtcattt gtcgctttcg tcagattacg atacgtattt actaatgtca gttcttcatc
     1321 cgagttagcc aaacgagaag ctacttctga aatcgtacgc tctacattgt ttctttcttt
     1381 tgccacaatc agattgatag atgatttata ggtaatgaca gcgaaaattg taaacacaac
     1441 gaatatgaag aaagaactcg caaaggccca cttgattgtc aaggagggcc ctttcagttc
     1501 tcttttttcc gattttttcg gctttatcac gagcgcatca cataacctgt tccgcgtaca
     1561 gtttggatat agctttcctc cccgggaacg tcaattttgt tgcgaagata acgaatatat
     1621 acgtccacta cgtttgtttc aacttctgtt tcgtatcccc acactttatt tagtaaaacg
     1681 tcacgtgcta ataccacgtt cacgttttcc attaaagtaa gcagcaactc gtattctctt
     1741 tttgttagtt caattacttc atttccgcga cgtacgacac ggttttcttt ttcgatagtc
     1801 aaatcgcgat aagtaatagt agtttgttta gccacatttt tatcgccttc aatatcgatt
     1861 cgacgcaaca atgcacgaag acgtgctaat aattcttcga tagcaaatgg tttgacgatg
     1921 tagtcatctg ctccgtggtc aagtccagat acacgatcga tcacagaatc tcttgcagtc
     1981 atcataatga ttggtgtatt tttgacttgg cgtacacgtc tgcagacttc taaaccgttc
     2041 aattctggga gcatcagatc caacaatatg gcatcccact cattatttaa tgccgcatct
     2101 aaaccagttc ggccgttgta gtggacttct gcgttatacc cttcatgctt caactctagc
     2161 tcgacaaatc gtgccaagtt cttttcatct tcaatgatta gaatattgct catttattat
     2221 tttatccttt ctattctact ggctttttaa aaactctatc attataccga taatatgctt
     2281 aaaaatctat agtcatttta tcatggatag caattttaaa aaagagctga agtctactca
     2341 taacaatcga cttcagctct ttcgtcctct ttttattttc tcatttccta acagccgaaa
     2401 gactattatt cttcatggta ccatgagtaa tggaagattc cttctttgtc gacacgctca
     2461 taggtatgtg cgccaaagta atcgcgttgg gcttggatta tatttgcagg cagacgttct
     2521 gctctgtatg aatcgaagta agcgatggct gatgagaaag tcggtacagg tacgccagct
     2581 tggacagcca aggcaacgac gtctctgact gattgctgat attttttcgt gatttcaacg
     2641 aagtaatcat ctagcaataa attttcgatc tcaggatttt tgtcataggc atccgtaatt
     2701 ttttgcaaga atcgagctcg gatgatacat ccagcgcgcc agatttttgc gatttcacca
     2761 aatggcaagt cccaatcaaa ttcttttgaa gcgacacgta attgagcaaa tccttgggca
     2821 taactcatga ttttactgaa atacaacgct tctcggattt tttcgaccaa ttcttttttg
     2881 tctcccgtga actcaaaatc gttcgtacga gacaaaatct tgcttgcttg tacgcgttct
     2941 tctttataag cagaaatata gcgggcaaat acagattcag tgatcaatgg aagtggcaca
     3001 cctaagtcaa gcgcgctttg gcttgtccat ttacctgttc ctttattccc agctgcatca
     3061 aggatcacat ctactactgg tttacctgtt ccttcatcgt ctttacgtgt cagaatgtca
     3121 gcagtgattt ctatcaaata gctgtctaat tcgccttgat tccattcttt aaagatatca
     3181 gccatttctt ctactgaaag gtttaaaata ttcttcatta agtcataaga ttcagcaatc
     3241 agttgcatat ctccatactc aataccgttg tgtaccattt tcacataatg tccggctcca
     3301 tttgggccaa tatatgtgac acatggtgct ccgtcttctg cttttgcaga aatttgctct
     3361 aagattggtg caactagcgc ataggcttct ttttgtccac caggcatgat tgaaggtcct
     3421 tttaaagctc cttcttcacc accggaaacg cctgtaccga taaagttgat gccagagttg
     3481 gctaactctt cattacgacg catcgtatct ttaaagaatg tatttcctcc gtcaatcaaa
     3541 atatcgcctt tatcaaggtg aggaagtaac tcttggattg ttgcatctgt tgccggtcct
     3601 gctttgacca ttagcaagat ccgacgtggt ttctcgatag attctacaaa ttcttcgatt
     3661 gtatatgttg ctttgaaatt tttatctggg tgttcttcta ctacagcggt tgttttagcc
     3721 cctgtacgat tgaataaagc cactgaatag ccgcggcttt ctatgttcaa ggctagattt
     3781 ttacccataa cggccatacc gacaacgccg atttgttgtt ttgacatatg aactcctcct
     3841 acatacggga tttctcccta atctcttaca ttatagctta acacggctac attgcaaact
     3901 aaagaacaac atatt
//
LOCUS       DACDYI010000127         3848 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.131, whole genome shotgun sequence.
ACCESSION   DACDYI010000127 DACDYI010000000
VERSION     DACDYI010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.131"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..382)
                     /locus_tag="IWM52_002799"
     CDS             complement(<1..382)
                     /locus_tag="IWM52_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9974977.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNK"
     gene            complement(413..1627)
                     /locus_tag="IWM52_002800"
     CDS             complement(413..1627)
                     /locus_tag="IWM52_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP9974978.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(1968..2210)
                     /locus_tag="IWM52_002801"
     CDS             complement(1968..2210)
                     /locus_tag="IWM52_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9974979.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(2242..2799)
                     /locus_tag="IWM52_002802"
     CDS             complement(2242..2799)
                     /locus_tag="IWM52_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9974980.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(2812..3000)
                     /locus_tag="IWM52_002803"
     CDS             complement(2812..3000)
                     /locus_tag="IWM52_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP9974981.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(3013..3579)
                     /gene="amaP"
                     /locus_tag="IWM52_002804"
     CDS             complement(3013..3579)
                     /gene="amaP"
                     /locus_tag="IWM52_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP9974982.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         1143 a          817 c          632 g         1256 t
ORIGIN      
        1 atttattgtt ttgtatagca ccaatacccg ttgcatagga taatgcacca gttaagaaat
       61 acagcatggt caagtagcca actgttatct caaaccattg gaaaaagatt gcggcaaaac
      121 cgaatcgttt tcccaatgtg cgactgaccc aagtatagac accgcctttt tcccatcctt
      181 tcaccgttgc catctctgct gcgcacaatg ccacagggat aaaccatagc aaaccgccta
      241 ataacaagaa aaatactaag ctgaagcctg aggtcgcgaa tgtgggatat tcatataatg
      301 aaacgaccat tgagatggtc atagccaaaa agccaaagaa cgttaatttt tttgcaggaa
      361 ttttcgtttt gctttgttgc atgaatcttc ctcttttcta tttgaatttg tttcaattga
      421 gtttgttgcc aaagaagaaa aagtccaatc cttcttcttg ttcttctttt gtagtagtga
      481 tcgttgtaaa gtgcatcgcc gattttaaaa tcactgttgt cagcacatag gcatacacgg
      541 cagtaatgat gacaccaatc acttgaatcc ctaagaagta ccagctgctt ggattcgtta
      601 gtaaatggct gttagatgca aagatcccta tcagaatcgt acctgtgaat ccgcccatgc
      661 catgaacacc ccacacatca agtgcatcat cccattgttt ctttttacga aaatcaacag
      721 cagcatgaca gacgataccc gcaatgatcc caatcagcat ggcacttttt gcttctacat
      781 aaccggcaca aggcgtaatg gctgccaatc cagcaacgga tcctgttagt acgtcgacaa
      841 aatcaaagcc atttcctttt acttttgcaa tgatcagcca agtaatcatc gcaaatgcaa
      901 gagcgatgaa tgttgaacca aatgcattga ctgcttgttc atctgctcga agtgcaccac
      961 cggaattaaa accgaaccag ccaaaccaaa gcagtcctgt accgatagca gctgccataa
     1021 gactgctatg ctgacttctt tctcctaaag gaatctttct ttgaccaagt gtgaggatac
     1081 atgctaaaga gccgaatcca gctgtcgtgt ggatgactgc tccaccagcg aaatctctaa
     1141 atcccatctg atccaaaaat ccgccacccc atatccagtg gcaaacaggg aaataaatca
     1201 gcagattcca aaaaataaca agtataatat agccttttaa attcaatctc cctgcaaaag
     1261 ctcctgtcat caaaggaacg gtaatcacga taaacatcag ttggtaaaga aagaacatta
     1321 aaaatggaat cgttgcacca tgaaacatgt tggggaagct tgtaacatgt ctcatcaaaa
     1381 agaaatctgc cgggtttcca ataacaccgc cgatatcccg tccaaaggcc aatccgaatc
     1441 caccaaatac ccacataatc gttgttatcc caacggagag aaaagattga gacatgatcg
     1501 ttagtatatt attcttccgt gctaatccgc cgtaaaaaaa agctagacca ggagtcatca
     1561 ggcaaaccat tgctgtacat aaaatcatga atgctgtatc tcctgtattg ataggtaatg
     1621 tattcatttt tttcgcctcc tttctacgat tacgagtata aaagaaggtg tagttgtttt
     1681 tttcttctgt ttttttgaat taatacttgc atttataaaa gacagacaat taataaaagc
     1741 ttgatgtgtt gtgctctttt tcgtttttca cacaaataaa gacaaaaaat aaacggtctt
     1801 ctttttatag aaaaccgttt attttcgtct ttttttattt ttaaagatat tttttcataa
     1861 ggaataaaga atacttcata taaaatatat aaaaaagaac cgcagcgagt gtcgctacgg
     1921 ttctcaaatt tgttcttcat ttcaacataa gtttttcttc aatttgctta taatttacgt
     1981 gcaaagaatg aaacgactaa tactaaaatg attgcaccga tcaatgatgg aatgatagcc
     2041 attcctgcaa ttgttggtcc ccaagttcct agtaaagctt gtccaattgc tgaacctact
     2101 aaaccagcaa tgatgtttgc gatgatcccc attgatgatc ctttattcgt gattgctcct
     2161 gcaattgctc caatgatacc gccgacgatt aatgcccaaa taaatcccat tttaaattcc
     2221 tcctaactga cttcttactt cttattgtac acgtggttcg ttattttctt tgactttttg
     2281 tgcacctttg cctacagcag attttgcttt atctgtttgg cgtgaagcaa attgtcctgt
     2341 ggattttgct gcttctgtta ctttgtcttg tactgtttct tgatcttttt ggtattcttc
     2401 ttgtgttttg atgtctgcaa cattcacatt cacttcgata acatctaaat gtgtcatatt
     2461 gtttacttca gtactgatca attctttgat ttggttgtaa atatcttcga tgtctttacc
     2521 gtattctacg atcacatcta gatccacagc tacttgtttc ttacctactt ctgtattgat
     2581 acctgctgtt acgttatctg tgttgactaa tttttcagcc atatttgaga aaaatccgcc
     2641 atctaatgtc aatagaccat ctacatcttt caatgcataa ccgatgattt tttggatgac
     2701 tttgtcttca taagtcaatt caccattcat catgcggttt tctccttttt catttgccaa
     2761 aactgatcca tttgttgtat tcatgttttt gttttccatc tttgtttcct cctatgattt
     2821 aaaaaattga tcaactattc ctgactgttt caaataaaaa ccgattcctg cgccaattgc
     2881 tgtcatgatt atgatcaaca atgtcttgaa aaatccaatc gttaaccata aaacagctag
     2941 cagtagtcca agacccccaa agataatagg gagcttgtat gctgaaaata gttctttcat
     3001 tatttggccc tcctattcca ctcgagagtg cttgtagtct cttgtatctt cttgttcttc
     3061 ataaccgctg tatgtgactg ccactttgac cgtttctttt gttccgagta ctttagttac
     3121 ttcttcttcg atatcttgca tcaatacacc tgttttccct actaatgaag aagttcgttt
     3181 caagtctccg ttgacatgga tatggattcg atttttcgtt gaacgtatcc gtactttagg
     3241 tgaatgaatg aactcttctt cgtgtaaatg ggaacgtacc aacccttcga tcgctttttt
     3301 gtcgattgtt agtttgccat cttcccgttt cataacgaaa gttcctcttg actttggata
     3361 gaaaagaacg actagcaaga tgataatagc aagaacggca aatactacag ctgcccagaa
     3421 aatatattgt tgcatgtagt aattcgttaa agtaaagaag cgaaatcgtt ccaattgttc
     3481 aggcataata tacggcgcat tgatcaacgc aatcgctaga aatactgaaa gtagcagtaa
     3541 actcatgata acaccgatta tttttaaccc cttattcatt ttccacctcc tataaactta
     3601 tttttaccct gtaaataact ctaccgacaa tcacagtata gatagcgaac agttagcata
     3661 gcatcagtaa ataaagcgct accaaacatt gctggatcat aacggcgttt tattgccagt
     3721 ctgatactga aataacaatt gcttacttat tctctattta cttttgattg ctggctatac
     3781 ttaaaggata ttattcaaga ttaaaaattg caaagattat gctcagttat tattacaaga
     3841 ttctattt
//
LOCUS       DACDYI010000128         3828 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.132, whole genome shotgun sequence.
ACCESSION   DACDYI010000128 DACDYI010000000
VERSION     DACDYI010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.132"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..293
                     /locus_tag="IWM52_002805"
     CDS             <1..293
                     /locus_tag="IWM52_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9974983.1"
                     /translation="VTDKAPSLGSAFRKLQSVGLYTKTEHRTVKYLNNLIEQDHRPIK
                     RRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEIKVLMGITA"
     gene            complement(350..781)
                     /locus_tag="IWM52_002806"
                     /pseudo
     CDS             complement(350..781)
                     /locus_tag="IWM52_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1340..1657)
                     /locus_tag="IWM52_002807"
     CDS             complement(1340..1657)
                     /locus_tag="IWM52_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP9974984.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            complement(1658..1912)
                     /locus_tag="IWM52_002808"
     CDS             complement(1658..1912)
                     /locus_tag="IWM52_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9974985.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            complement(2172..2390)
                     /locus_tag="IWM52_002809"
                     /pseudo
     CDS             complement(2172..2390)
                     /locus_tag="IWM52_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT         1277 a          646 c          548 g         1357 t
ORIGIN      
        1 ttgtgaccga taaagcacct tctcttggct ccgcctttag aaagttacag agtgtgggtt
       61 tatatactaa gacagagcac cgaactgtga agtatcttaa caatttaata gaacaagacc
      121 atcgacctat taaacgacga aataaatttt atcaaagtct ccgtacagcc tcttccacga
      181 ttaagggcat ggagaccatt cgaggaatat ataaaaagaa ccgaagaaat ggaacgctct
      241 tcggcttttc ggtgtctact gaaatcaagg tattaatggg aataacagcc taagatattt
      301 ggagttcaga gagggcgcgt ttgattttca aacttcgcaa cagaaccttc atatttcctt
      361 gattacctga ggcaatgaaa gacttatcta cttgaattag ggtgaaattc tcattcaaat
      421 atggtagtaa aattaatata tgtgaaaatc gaatcttgag atagctattc cccttgagat
      481 attcactgat acttctactc aaatcattta aatttttata attatttgta cggaacgtat
      541 atatatcaac atcagttttc tttacatgag aacttattct aaaatgaaaa agcctactct
      601 cagaaagaat tgaaaaatag atactttcag tagatactga agattaaaca aaattaaact
      661 tgtatccttc aggcatagca cttttaacag tctgctgcat taactcggat aatcttttga
      721 aagtagtaat atatttttta ccagcaaacc atacatatct tttttcatta agtatgttca
      781 ctatctctcc tccattaatt atatttagca tcaaatagtt attgtaccta tattttatcc
      841 cattcctttt aaaacaagaa tacgtacaaa gtaaaatgtt gtaaatagtc tagcagggat
      901 gtatattttc atgtaccaac ataatattta agaagtttcc catagatata ttggacttcc
      961 tcttttaaaa cacaaaaata aatactacct tatttaggta gtatttatta aaagttacgt
     1021 caattttgat taaaaatata ctacttgaac ctctcatgag taaagtcacg aaatttttgg
     1081 gaatagagcg tacttgatat cttatttttt cactaatagc agtgtctctt attcaccaag
     1141 cgatccccgt agttcctacg gttcttaatt tttctctaga ctaatgattg tatctttaga
     1201 ccttcggcca taacatggag caaaataaaa aggtactaag tcttatatat caatcttcga
     1261 gtaaccgacg gttaacaacc atatcgagcg gcattaatca cccacctgta gaggatgagc
     1321 gacttttgcc taattattgt taaaagccta gtttcatttg tttagattgg aaaatacgtc
     1381 ctacaagtat ctcttttttt tcatggtcga tccgataaaa taaggcatag cttttgccga
     1441 tcacgatccg atatgttggg atatccattc catagatttc ggaaacttcc tcatgcatag
     1501 ttggaaaatt tttggttagt tctaaagcct tctggatcgc acctacaaat ctttttatgc
     1561 tggctggaga aagagcaagc tcattttccc attcttctat tatcgtttca aggctggtct
     1621 ggaaactttt agaataaact agcgtgtact ctttcatcta ttcactccag ccgtattttc
     1681 cgaaagcttt taccatttca atttctgaaa ctgttaaacc tttctcaaag tcttggaaac
     1741 tttcttcaat caatttgcga tcgtgttctt ttgctatttg agtagtatct aatatgaata
     1801 agttgccttc ttgtttggct tggatagtgt ctccgctttt taatccaaga gagcttggaa
     1861 ggatcactcc cacgctattg ccgatctttc taacctttag ttcttttccc atgttatcac
     1921 acttctttta gtttttgata aataaattat aacatgttat aacgacaatt gccaacggtg
     1981 tttcttaagg gattccactc catattatta ttggacaaaa taagccactt agaacgctga
     2041 aaacatatac aaacgatatc aaaatacgag tgaactactc accatacact tccgttaaca
     2101 tccatttaag taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga
     2161 tatagctaaa gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg
     2221 ttctcggcta attcataaaa attgttatgt gttccatata gcaatactgg aataacttta
     2281 ttttcttgaa tctcataagc aactctatac acggtgaccg catacttaaa tccattagac
     2341 caattacctg ataaacctcc agttttttgc tcttacttaa atggatcaat agcaatttca
     2401 tcacaaacct atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta
     2461 actctatgta gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc
     2521 taaaaatata tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa
     2581 aatcaataca taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa
     2641 tagttttgct gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa
     2701 tacctttttg attttgtttt tgcaatcaag ccatttgcag aggtaactgc acactacatc
     2761 tgcaagtggc ttgattgcaa aattgccaaa aaagacagta agcctaccag ttaattctat
     2821 aatatagaaa cgtaaaaaaa aaccccatca gcctacacgc tgatggggtt tgatgatata
     2881 atgaaatacc tctttgttac tcataattat accataatta gtcacgaatg caaccaatag
     2941 aaaaaactac ttagaacggt agatcatcgc taataacaat gcattgtcca ccaaatggat
     3001 tttgtgaagc attaaataca ttgttattaa ttgtcgctgc ttttaactag gcctctcccc
     3061 ttctattata aaagaagata tatattattt cttaattacc tactacctta aaccaaaatt
     3121 taagtactac ctaatttaag tagtacttaa ataatctaaa aaatagttat cccaattgtt
     3181 atgcttttat aatacgatta tttgtaacaa aatgaatatt atataattaa acactaccat
     3241 atttaggtag tgtttaatta ttagcaaaac aaaaaatatt tattctattc tcttaacttc
     3301 aactaagtta tgctgcacac ctttatataa atcatatgca actcattagc tagacaaact
     3361 acaagcatgt agttttatct gttaattatg tactacctaa ataaagtagt ttataattaa
     3421 caggtatata tatttaaaaa tttaagatta atttttttat gacttgtaca aatttatctg
     3481 atattctctt attaagaact acctaatttg ggtagttctt attttttaca ttttaagtag
     3541 tatttaattg caatttggta gttttttttt attatcttat tcctagaacg gtagtactta
     3601 atataaatat aggtagtatc taatttaagt attaatggta gtatctaatt taacaggtag
     3661 tatgtaacta ctttaaagta atttttttca gattaacaca gtagtatctt attaaataag
     3721 ttaattttaa tggtagtaca taattattat atatatctga cattggtagt acttattgta
     3781 ttttttagta gtatataatc tattgttttc tagcctaaag ccttgtct
//
LOCUS       DACDYI010000129         3733 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.134, whole genome shotgun sequence.
ACCESSION   DACDYI010000129 DACDYI010000000
VERSION     DACDYI010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.134"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="IWM52_002810"
     CDS             complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="IWM52_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP9974986.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            1228..2331
                     /locus_tag="IWM52_002811"
     CDS             1228..2331
                     /locus_tag="IWM52_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9974987.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            complement(2524..3591)
                     /gene="rlmN"
                     /locus_tag="IWM52_002812"
     CDS             complement(2524..3591)
                     /gene="rlmN"
                     /locus_tag="IWM52_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP9974988.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
BASE COUNT         1131 a          748 c          635 g         1219 t
ORIGIN      
        1 gtacacggtg ttccaaaagt agcttcttcg gaaataagtt ggaatttcac aaaaattcaa
       61 gaagtaattt tcgtaaattc cttcttattt cttgaagctg attacttctg tcacaacctc
      121 tttttattat tgagaatttg gtcgaggaat aatcgttttt gccatccaaa tatccggttt
      181 gtcccagcca tttgcatttg gtaagacacc tacgatttta taacctaatt tttcatagaa
      241 ttcatacgga tgttcacgaa ggttctggat ggaagccact ttatcaaatg tatgctcata
      301 caggtccgtt tgacttaacg ttgttccatg gtctaaatca tccgtaccta aataaatcgt
      361 gattcctcct ctggaagcta cttctttttc taagtaattg actaatcgag tacctatttg
      421 gttctttcgt cgggagcttt ctacaactaa tggatgcaat tcccaacctg tgataccgta
      481 ttgagggatt gcaccaataa atcctactaa ctcatcttgg tctaccgctg ctaccgcgat
      541 tcgttctgga ttcatcattt cttctacttc ttctgccgag ctgtctccat attcttccgg
      601 ccaagtcagt ctcagtaaat cagaaagctg atctttcaat actggattat tacggtcaaa
      661 ttcactgatt atcatacgtc ttcccccttg ttcttgctta ttctaacagt ccttttgaaa
      721 aaacgaaatg ttctcgctca aaaatcttta gctaccaatc agtatctcta ttctttcatt
      781 atttatctta cttataaaat aaaaaaatta tacagaaaca ctttattcaa aataggaaag
      841 atcaggaaag ccatcaattg gaggaaaaaa ctctgtttgt tcatcatgat atagattatc
      901 ttttgcttta aaaaaaacca attatgaatt gacaaataaa acctgttttc atataataat
      961 cttaattttt agaattttta gataattaca aaaaagcaaa gggaatcggt agaaatacta
     1021 agaggaacaa aaattcatta ttgtcgtttt caatgttttt gtacttgttc gtttttagga
     1081 tacttattga tgatttcaag gtgttcatgc caccttattc atcacttatc ctgtcttagg
     1141 ttcacctcta ctccatctac agccgcagtc agatcgatca ctctttaaaa ttttaacccg
     1201 tttcaacgaa gaaagaagga aatgaatatg agtcaaagtg ttttcttacc aaattatacc
     1261 attggaacag atgcgtatga aaaagcaaat ggaatcgtca aagaatatgg gacaaaagta
     1321 gtgctgattg gagggaagac tgcactttca aaggcagaaa acaaaataaa agtagctttg
     1381 acacaggaaa tagaggttgt cgccagtctc tggtacgggg gtaattcaac ttatggaaat
     1441 attgaacagt tgatccagca tgaaagtgtc caaaaagcag acgtcctgtt tgcagtcggt
     1501 ggtggccgtg cttgcgatac tgtaaaagtc attggtgaaa aactagctaa accagtcttc
     1561 acctttccca ctattggttc gaactgttcg cccgtcactg cggtatgtgt catctataac
     1621 gaagaaggcg taatggacgg actcttcttt ccttcaaaac cacctgtaca caccttcatt
     1681 gatacagcaa ttattgccga agcacctgat aaatacttat gggcaggtat tggcgatgct
     1741 ttatccaaag aagtcgaatc tactttttct gctcgtggcg atgatctagg ctacacggat
     1801 agattaggaa tccaaatcgg aaaaatgtgc attgatcgtc tcttgactta tggtacgcaa
     1861 gcattgaaag atagtgccga cgatcgtccc tcatatgcaa ttgaacaagt cgtttcggat
     1921 atcattgtta ctaccggatt ggtttctgtt ttagttaaca ataactataa ttctgctatg
     1981 gcacactcat tctattacgg tacgactgtt ctagaaaaaa tggagggata ccttcacgga
     2041 gcatctgtca gttatggtgt actgacctta ttgatgatgg atcagcagtt cgatttgttt
     2101 gataaaatct atgtgtttat gaaaaagaac caacttccag tatcgttaac cgacatgaat
     2161 ctcaatgtca cgaccgattt agaagcaatc ttagataaag ccatggcaac aaatgatatt
     2221 aaacatgtgc cttatgaaat cacccgagaa aaatttaaac aggccattct tgacgtagaa
     2281 acttatcatg ctacacaaga aagccagcaa ggatataaga aaatcagtta aataattgct
     2341 aaaaccccca tcaagcaggc tgtcatcaaa ttgaggacat ttctttgctt gatgagggtt
     2401 tttcttgtca aaaaaagctt tatagctttc gctatagaat cttttctctt atttaagttg
     2461 aacagttcct taattaagaa aactgctcct tttatggtaa ttgaaaaaac gagtaggata
     2521 cgattatttg gcaactgttt ttttcatttg cttgcttcgc aattgtccgc aagctgcatc
     2581 aatatctgta ccatgttctt tacgtatcac acagtttacg ccgtttttct tcaatacatc
     2641 ataaaatctc aatacatcag cttttggact gcgagaatat tgatcatgtt cactgactgg
     2701 attatatgga atcaagttga catatgctaa ttttttctta tcttttaata gatctgctaa
     2761 ttgttgcgca tgctctggtc tatcatttac ttgattcaac atgatatatt caaacgtgat
     2821 tcgacggttt gttttctcta aatactcatc cacagcttcc atcaatttct caattgggaa
     2881 acttcggttg atccgcatga tcgatgttcg cacctcatta ttcggcgcgt gcaaggaaat
     2941 agccagattg acttgcaagc cgttattagc gaattccttg attttgtgtg ctaatccact
     3001 agtggaaact gtaatgtgcc gagcaccaat tgccaatcct ttggcatcat tgatgatatg
     3061 caaaaagtcc attacattgt cgtaattatc aaatggctca ccgatcccca ttacaacgac
     3121 atgtgaaacg cgttcgccaa gattgcgctc atcaaaataa tgttggacca tcatgatctg
     3181 tgcaacaatt tctccagcag tcagatcacg gttctttttg agcagcccgc ttgcacaaaa
     3241 cgtacagccg atattacatc ctacttgtgt cgtcacacaa actgacaagc catattcttg
     3301 tctcatcagt acagtctcaa tcatgttttt atcagggagt tcaaacaaat attttactgt
     3361 accatccgaa gcttcttgga cgaccatctg ttttagagga ttgatgataa aatgctccga
     3421 taatttttcg atcaattgtt tcgatagatt cgtcatttct gtgaaattac ttacacgttt
     3481 ttgatacaac cattcccaaa cttgggcagc tcggaatttt ttctcgccgt tttctataaa
     3541 ccaattgatt aattcttcat tcgtcaagcc ataaatggac tgtttttcca aagtattttc
     3601 cctctttttc tcaaagtttc gctacatact atacatgatt catttcctta atgcaatact
     3661 tcacaaataa atcgaataga actttaatct cacaaaaact aaaaagacaa gacatcatac
     3721 cgtattagaa aag
//
LOCUS       DACDYI010000130         3464 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.135, whole genome shotgun sequence.
ACCESSION   DACDYI010000130 DACDYI010000000
VERSION     DACDYI010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.135"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            361..642
                     /locus_tag="IWM52_002813"
     CDS             361..642
                     /locus_tag="IWM52_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974989.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            644..1060
                     /locus_tag="IWM52_002814"
     CDS             644..1060
                     /locus_tag="IWM52_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974990.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            1682..2836
                     /locus_tag="IWM52_002815"
     CDS             1682..2836
                     /locus_tag="IWM52_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAP9974991.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            2830..3252
                     /locus_tag="IWM52_002816"
     CDS             2830..3252
                     /locus_tag="IWM52_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974992.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
BASE COUNT         1308 a          595 c          535 g         1026 t
ORIGIN      
        1 ttaataaaaa ttgctggttt tattcttctt tttgcttttg cgagcagagc aacatggacc
       61 catgttgcga tattttggtg gcgccccaat ccccgttttt tcgttgaatc ttactcggac
      121 tgcactcgtt ttacagctga tttttctgaa ttttgaaaga gcataaggaa acgtggtatc
      181 actttcttac gaaaggtgct agataggctg tgcctatcac gtttctcaga aaaaaatttg
      241 ctatcacttt tttgccattg tggctaggct atgtatagtg ttttggcgaa aaatagctat
      301 acaaatacta tatttgtatg gtatacataa attatacgct gatacgaaag gaaaatttcc
      361 atggacattt tagttcgaaa tatcaatccc aaaattattg caagaataga taaacgttgt
      421 caggaactca caaccaaaac gggcaaaaaa tggagtcgaa ataaatatct aaaagttcta
      481 atcgaaagtg attttgaccg tcaacttatg aattacaaaa aagatcaatt cgatttatta
      541 ctcgaaaaat ttattgccat tcaaacagaa aatacgaaca tactgaatga ctatgttcac
      601 acaaacaacc aactcattga aacacttatc ggaggaatat aacatgagaa aaacttctgt
      661 tcaaaaacac gtaacccttt atcccagtca cgttgacgtt ttagattcca tcatcgaaaa
      721 aaatccagac gtcaacaatt attcagaagc cattcgtttt ttatgtatga gttatgaacc
      781 tgataccaat acaaccacaa ccagtaacca agcaaaactc aatgccatgg gaaaagaaat
      841 ttctatcatg acagaaatca tggcagaatt tgctgattta tatctaaaag atactggtgt
      901 tcttatcggt acaaattcta aagtgtatca agaagtgaaa gaaaaaattg aaaatcaact
      961 caatccttcc cgacgaaatc aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac
     1021 ttctggaaat tttgacccaa caaactttct aaaaagataa aaaaagaaac cgatccctaa
     1081 aaaaattcct cgatttcttt ttagggatca gtcttccagc aagatttgca agttggtacc
     1141 aacttgcgaa aaaaaagaaa aatccgctta tgcaacttct tctttttgta ttttgaggcg
     1201 ctaccccaat ccccaagtat cggttattta atagcgcgga aatccaaaga ataggacgta
     1261 ccctgaaaca aaaaaagact gctctcacga gcagcccaaa atgagattat cctattcatc
     1321 aacataaaaa tattacaaag tgaagaacta gagaaagaca aaaaaagcat atcaaatcct
     1381 ctcaaaaata acaagtcttt aaaaaatgac agataaaaat gcacaaagaa ctaaaaaaga
     1441 gaaatcgcgt aaaatcaagc attagaagct agtaactagc tagaccaatt aaaaatcaca
     1501 aagtgagcgt ctctatccat tggtagaggg gctacttggc atagtgtaca ttatgttaac
     1561 taacgctata aataacgatt atgttcccaa aacaatattt tatgaactat aacaaaaatt
     1621 tgttatcgca tattgtatat tttaattata ggtgtatacc acttaaattt aacacaaagg
     1681 cgtgattatt ttgaaaccca aaaagaaaat ttatctaggt ctcctctttt ccttgatgat
     1741 tggaattttt agtataggaa ttacaaatac ctatgcttca gaaaattcta atttcaatat
     1801 caactcccaa ccaattgaag aacaagcaat taattttgcg aaaactgatt ttgaacactc
     1861 cataacttca attcttttaa tggaaaataa caccgaaact gatattaact atactcttgg
     1921 ttcgccattt aaaatgctcg atggagaaaa tacggcaaat agttctattt tctttccagt
     1981 attttcagac gctgataata cgattaaata tatttatacc gtcaaaaaaa atacaaatgg
     2041 agaatattca ggaagtattt cacaattttt agcaaaagaa cttcaaaatc tagtagatag
     2101 taatataaca gatacaacct tattttttca agataatagc atttattatt ccgataatgg
     2161 acaaattaaa ctactttgga aatcaccttt aagtacagaa aaaaatggtg tttcttcctt
     2221 tacagaaaaa aatgatctta tatccgtaaa tataaaaaat aaaattaaaa atttctctaa
     2281 aaaacaaaat tttcctgcgg ttaaacgtgt aaaaagtgtc tcctccggag ataactttat
     2341 tatgattaat tggcgtatag cagaaaccca aacaagtttt ccgtggtgtg ctgcttacgt
     2401 agaagctgct atcttatgta acaaaacaga tgtacgtgct acttcagcta gagccattat
     2461 gcattatacc tatcctaatt taaacaacag tcaaattgaa tcaaaaagtc ttaatcaaga
     2521 tcaaataata aaatatgcaa attactgtgg tgtttatcct aaaaaagtag cacgaatact
     2581 aaacctttca gaggtccaaa cacaactaag aaaaggaaat gccatttata tgggaactgc
     2641 tggttatgga aattatagta acagccgtca tgcacttgca ttatttgggt gggtcaatca
     2701 aggtggttat caaacttact atgtgtggaa tccttggtat aactacccaa ctgtaataag
     2761 tgcaaatgca ggaccagtta caattcctgt acctggaggt ggttttactt gggacaatac
     2821 cattacaaat tggtaaataa acccattgct ttagttatgg gtctagtgtt agttcttttc
     2881 ttaactagtt gttcaaaaaa acaagaaata caaaagactg atctgattcc ttttctagga
     2941 acttggcaaa ctaaagatca ttcagaacgt ctagtaatca ctaatttaaa tacttcaaaa
     3001 aatatagaac aatataccat cttagtaaaa gagaacaatc ataaaattca aatcattgat
     3061 aaaaatttaa atataaatga acaagttttc atttacaaag caacaaataa tttactaaca
     3121 ctgcgctcta ataaaaaaca attagattac acttttagtt tggattctcc aaaaaagctc
     3181 gtatttattt tgacaacgaa tgatgggaca gaagggacag ctaaaccaat tgaatttttt
     3241 aaagctgatt gatgaatagt tgtttgatat tacaaaacaa taatttcacc gaaggtgaaa
     3301 caagcgaaac tttacaaaaa gtggctagca tttgctagcc ttttttgtat tgtggagcgt
     3361 gggttttgag ggggattcaa gggggagctt gcgccccttt gaaaagcggt tatttgcacc
     3421 aaatacgtag gtatttggct agcaaatata gagcttgcca aaca
//
LOCUS       DACDYI010000131         3292 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.137, whole genome shotgun sequence.
ACCESSION   DACDYI010000131 DACDYI010000000
VERSION     DACDYI010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.137"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            37..588
                     /locus_tag="IWM52_002817"
     CDS             37..588
                     /locus_tag="IWM52_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="HAP9974993.1"
                     /translation="MKRLEASTSNFNNKLDVFEEKTKHARLNFETTAKHYIKRLDEDN
                     LKLDFQQAIQDELSDTKDEIREVTKQAREETKEYKEILESKIKDHNKVVDKSNTALKV
                     MTKGVTNIFFVLIIFVLVMLVTGPIGHFFGIEHLYSFINGFIDDHESAWRYLMLILYA
                     VPYVFFAIILWLIALFFRVFNDL"
     gene            1264..1530
                     /locus_tag="IWM52_002818"
     CDS             1264..1530
                     /locus_tag="IWM52_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9974994.1"
                     /translation="MAKITKNEQENLVENYTKKPAYSNSRLDAHFVCTSNPKLSFDAM
                     TIVGNLNKNSAKKLSDFMSLDPQIRLWDILQTKFKAKALQDPLC"
     gene            1588..2100
                     /locus_tag="IWM52_002819"
     CDS             1588..2100
                     /locus_tag="IWM52_002819"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003321317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9974995.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLC
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            2097..3134
                     /locus_tag="IWM52_002820"
     CDS             2097..3134
                     /locus_tag="IWM52_002820"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9974996.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1213 a          555 c          653 g          871 t
ORIGIN      
        1 agcgagcaag acaggaacga gagaacaaac atactcatga aaagactaga ggcttcgacc
       61 tcgaacttta ataataagtt agatgttttt gaagaaaaga ctaagcatgc aagattaaac
      121 tttgaaacaa ctgcaaagca ctatattaaa cgacttgatg aagataattt gaaattagat
      181 tttcaacaag ctattcaaga tgaattaagc gatactaaag acgaaatacg tgaagtgacg
      241 aaacaagcga gagaagaaac aaaagagtat aaagaaatct tagaaagtaa aataaaagac
      301 cataacaaag tggttgataa atcgaataca gcgctaaaag tgatgacaaa aggcgttaca
      361 aatatatttt ttgtgctaat tatttttgta cttgtaatgc ttgtgacagg tccgataggt
      421 catttctttg gtattgaaca tttatatagc tttattaatg gttttattga tgaccacgaa
      481 agtgcatggc gatatttgat gttgatactt tatgcagtac cctatgtatt cttcgctatc
      541 attctgtggc tcatagcatt gttttttaga gtatttaatg acctttaggt agatttatat
      601 ataaaaaggg ctaaatacgc aaaatacccc cttaaaatgc aaaataaatg caaataaggg
      661 ggtaactcct tatgaaaaaa caactaatag tattataaca cgtttttatg ccgagaaaac
      721 ttattggttg gaatgggcta tgtgttagct aacttgttag cgagttggtt ggacttgaat
      781 tgggattaat cccaagaaag taccaactca acaacacata aagccctgta ggttccgacc
      841 aataaggaaa ttggaataaa gcaacgaaag gagttgagta aaatgagtga gaaaacgaag
      901 aaaatcattc gagtatcatt agctttttca caagtagtag tgcaatcatt gatagtttat
      961 tatacttacc agcaatataa attatctaaa gagaattaag ttttgattat gtaataagtt
     1021 ttggtactaa aaaagacttg atctaattag accaagtctt ttgttagtgt tatattaata
     1081 acaaaataaa aataagaagt cgctcactcc ccgaccaaag tttgtgagca aaataaacag
     1141 tcgatgtttg tttcttaatt gtatatcttg atattaaacg atttaagatt attcttcaag
     1201 atatatattc gcaaatgtaa ttggaaaagc gacttcttaa acaaaattaa ggagtcgctt
     1261 tttatggcaa aaataacgaa aaatgagcaa gaaaatttgg tggaaaatta cacaaaaaaa
     1321 ccggcgtact ctaatagccg gttagacgca catttcgtgt gcacttctaa tccgaaattg
     1381 agctttgacg ctatgacgat agtcggaaac cttaataaaa acagcgctaa aaagctatcc
     1441 gattttatga gtttagatcc acaaattaga ctttgggata tacttcaaac aaaatttaaa
     1501 gctaaagcac tacaagaccc cctatgttag gctagttgtc gtataggata ataagttata
     1561 ataaagacta tacggaggat gattaccatg acaaataaaa acaatgaacg ttactctacc
     1621 gaactgaaag cgacagccat caagaaaatg atgccacccg aaaatagggg gattcgccaa
     1681 ttaagtcaaa ctttgggcat tccagaagcg actctttgtt cttggcgtaa aaaagctaga
     1741 attcaaggga atactacacc cggaacgaaa aaaaatgtcg aaaaatggtc aagtgaagat
     1801 aaatttttaa ttgtgatgga atcattctca atgaaccaag tagaacttgc ggaatattgc
     1861 cgaaagaagg gcttatttaa agaacaaatt gaggcttgga agaaaacgtg cttatctgcg
     1921 aatgaacagg aagaaaatcg aacgagagaa cttgcaactg aactaaaaga agagaaaaaa
     1981 caagcgcgtc aattagagaa agaattacgt cataaggaaa aagcattagc ggaagccgct
     2041 gctctcttat tactaagaaa aaaagcacac gcgatttggg gggaccaaga ggaagaatga
     2101 tttcatcttc agatcgcaaa caagctgttg aacttattca agaagccaat cgcaatgggg
     2161 cccgtctcac ctatgcctgt aatgagctga atattagcgt tcgaacctac gaacgatgga
     2221 cgcgtgaagg tacgatagcg catgatcaac gccctttggc gaaacgtcct gtacccaaaa
     2281 ataaactgac ggatcaagaa cgcgaaaaaa taatagaaac cgtttcaaaa aaagaattta
     2341 tgaatttgcc acctagccaa attgtaccaa aacttgcgga ttgctcgatt tatattgcat
     2401 cagagtctag cttctatcgt gtattgcgag aaaaaaatat gcaacatcac cgtggccgaa
     2461 gtcaagtttc acaaaaacgc ataccgccaa gtcaccttgc gacgaagcca aatgaagttt
     2521 ggacttggga tattacttgg ctaaaaggtc caataaaagg actcttttac cggttatatc
     2581 tcatcattga tttatttagc cgtaaaatgg taggctggga gatttgggaa tcagaagaag
     2641 ctcgtcatgc cgaaaaactt attaaacaga cggtgcttaa ggaacgtatc caaggacagc
     2701 cgttggtttt acactccgat aatggcagcc ctatgaaagc cgcgactttt caagtcttac
     2761 tcgaaaaatt aggaattcaa agttcttatt caagaccacg agtgagtaat gacaatcctt
     2821 attcggaagc ggctttccga acgttaaaat atcgaccaga atatcccaat aacggcttta
     2881 aaacaataga tgatgctcga aaatggacga aagagtttgt cgattggtat aacgattctc
     2941 atttgcatag tgggattcaa tttgtgtctc ctgcagcgtg tcatgaagga cgacatctcg
     3001 ctattttaga gaaacgaaag caggtttacg aggacgctaa aaagcgccat cctgaacgct
     3061 ggtcaaaagg tgttcgaaat tggactccac atcaatcggt ggcgttgaat cctttaaaag
     3121 aaacagaaag ttaacaggag cataatcttt caagattatc aaataaccct cgtcacccaa
     3181 taggggaaaa agttattctt cccctattgg ataacgaggc caaccgacca tagggaggtt
     3241 ctgctttttg ataggtggaa aataaaaata cgacaactat attgacaaac ac
//
LOCUS       DACDYI010000132         3282 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.138, whole genome shotgun sequence.
ACCESSION   DACDYI010000132 DACDYI010000000
VERSION     DACDYI010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.138"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            1..585
                     /locus_tag="IWM52_002821"
     CDS             1..585
                     /locus_tag="IWM52_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9974997.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            complement(662..1837)
                     /locus_tag="IWM52_002822"
     CDS             complement(662..1837)
                     /locus_tag="IWM52_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9974998.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            1919..2299
                     /locus_tag="IWM52_002823"
     CDS             1919..2299
                     /locus_tag="IWM52_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAP9974999.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            2373..2735
                     /locus_tag="IWM52_002824"
     CDS             2373..2735
                     /locus_tag="IWM52_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9975000.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
BASE COUNT         1114 a          539 c          622 g         1007 t
ORIGIN      
        1 atggcattcc cacaactaga tttaaaaagt gaaaaaggac caaaagctgt tatcaaaaca
       61 aaccgtggtg acattactgt tcaattattc cctgaactag caccgaagac ggttcaaaat
      121 ttcattgaac tttccaaaaa aggttattat gatggcgtga tttttcaccg cgttattcct
      181 gatttcatga tccaaggcgg cgatccaact ggaacaggaa tgggtggcga aagtatttat
      241 ggcgaaagtt ttgaagatga attcagtcgt gaactgttca accttcgcgg agctttatct
      301 atggcaaatt caggtccaaa cacaaacgga agccaattct ttattgtaaa taacacaaat
      361 gtgccagcta atatgcttgg acaactagaa ggtgctggat tcccatcaga aatcattgaa
      421 gcgtataaag gtggcggaac tccttggcta gatttccgac atacagtctt tggacatgta
      481 ctagaaggga tggacacagt agatgaaatc gcaaatgtac aacgtggtcc acaagaccga
      541 cctgtacatg atgtagtgat tgaaacaatt gaaatttcag aatagtttgg tatggtacgg
      601 agtaattatg aaaaaggacg aaacctagta aggtctcgtc cttttatttt gaataaatga
      661 tttaatcgat tgtctctaaa gcatacttga ttcgattaag tccttccagt aaagtttctt
      721 ctggacaagc aatattcaag cgcatatgcc catgaccgga aggaccaaac gtgatgccag
      781 gatttaatac gacttttcct ttccgaacaa gagtgttctc caattcttgg tcgtctgaaa
      841 tataggcggt aaaatctaac cacattaagt aagttccttc agggttcatt attctcactt
      901 tcggaagata ctgttcaaaa aactgattaa ctgtttgaac atttttttct agataaggaa
      961 tcagttggct taaccattct ccacccgtct cataagccgc ttgggttcca atcagtccaa
     1021 aagtgttgat ttcgtgctgt tggttcatca ctaaatgttt ttcaaaatca gctttcatct
     1081 ctggattttt gataaatacc attgaatttt tgattgcagc tagattgaaa gtcttggttg
     1141 cggaagtaaa agcaactagt agatcatcta aattaggatc aatcgttagc atggaagtaa
     1201 atgtatgatc gaataaagtt aaatcttgat ggatctcgtc actaaacaga atcacttgat
     1261 gttttaaaca caattcgctt aactgtttca attcttcttt gctccaaaca cgccctccag
     1321 gattgtgagg attgcataaa atcattgctt ttacattgtt ttcttcgatt tttttctcca
     1381 tgtcagacaa gtccatgatg aaatgattat ttttttctag caaagagctt cgaacgatgt
     1441 gacgttgatt tgtttctata atagaggaaa atggaggata aactgggtca tgaatcaaaa
     1501 tagaatcgcc tggttttgta aaagtttgta ctgctgcagc taaactggtt aacacaccac
     1561 ttgtaaaaac gatattttct ttcgtcaact ccacagcatg gtgttggcgt tcccaatgaa
     1621 tgatagcctg atatagttta tcagacagag tggcataacc aaagattcca tgtttgatgt
     1681 attcactaaa cgctttagct accccaccgg gagacaagaa gtccatatct gctacccaca
     1741 aaggaagtaa gtcatccatt tgatatgtct cagcaattgc gtcccatttt acactttctg
     1801 tatcttttct tgaaatctgt ttatcaaatt cgatcatctt aagacctcca gtcattttcg
     1861 tcgcttcttt ttttgagcta tttacagtat aatgataaat gcgaagagag ggaagtagat
     1921 gacttataaa attggacaaa taattgaagg aaaagtgaca gggatacagc cttatggcgc
     1981 attcgtttct cttgataacg agacgcaagg acttattcat gtttcagaag tacaatccgg
     2041 ttatacgaaa aatatccatt cattgttaaa agtcggacag ccagtaacag ttcagatcat
     2101 cgacattgat gaatattcca aaaaaatcag tttatcttta agaacgttag aaaaaacaaa
     2161 tccagctgtt ccatatcgca gaaaaaggta tttcacgaat aaaaatcgta agatcggatt
     2221 taccactatt gcggagcagt tacctatttg gatagaagaa gcattatctg aactagaacg
     2281 gaaagaaaat aagaaataaa aagtgtattt tttctgaaaa tgtgctagaa tttttatgta
     2341 agccatagaa caagttgatc gaggtggaaa ttttggcaag caaaagagtt gctggtacaa
     2401 ttatgttgca tttggaggat ggtactaaaa aatttttggt ccattcaatt gatgacaagt
     2461 tggaatttgc gttagcggaa ctttctgaag gaaagactgg tttagcgaat atacttaatt
     2521 tcttaaaaga aatagttcat atcgatgtta gcaagattag tttgatggaa ttgacaaata
     2581 cacatattaa agaggaaaat gttccgttgt ttgtctttga aactgaacaa aagaatcaaa
     2641 gagaagaatt aggcgaagga tatatttggg aagaaccagg tcaaatgcgc tctgttcttg
     2701 gaacttacga agtagaggga gtgcctttct tctaaaatac agattaagcg aacaaagaag
     2761 tttgaatttt tttcaaactt ctttttttat tgtttggtcc aaaaaataca atgaaatgtt
     2821 ctgctaaaga tagtttttta gtgcatttta ttttcaaaat atgttgaaaa cggttgtata
     2881 acgaataata ataattgatc gaaaaatcat ttcacaaagt aatgcttaat ttatagttga
     2941 ccaaggaaga aatacatgat aaatttaaca gcgttgattc tgaagtggat gttatatcac
     3001 atttctaaaa aatttaaaaa atagttattg acatgttact aacgacatga taagataact
     3061 gagtcgttaa aaaagaacca cttgcgaagc ttgattctat cgaaaaaaac ttcaaaaaag
     3121 ttcttgacaa ataacaaacg ataagttatg ataaatgagt tgctgaaaga tttcgaaaaa
     3181 cgaaatcgaa aaaaactttt aaaaaagagt tgacaatcgc ttgtctctct gatatgatat
     3241 aaaagttgct acggcagcca aaaacgaatc gggaaacact cc
//
LOCUS       DACDYI010000133         3154 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.139, whole genome shotgun sequence.
ACCESSION   DACDYI010000133 DACDYI010000000
VERSION     DACDYI010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.139"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            274..357
                     /locus_tag="IWM52_002825"
     CDS             274..357
                     /locus_tag="IWM52_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAP9975001.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            482..1219
                     /gene="erm(B)"
                     /locus_tag="IWM52_002826"
     CDS             482..1219
                     /gene="erm(B)"
                     /locus_tag="IWM52_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP9975002.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1482..1598)
                     /locus_tag="IWM52_002827"
     CDS             complement(1482..1598)
                     /locus_tag="IWM52_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta-toxin"
                     /protein_id="HAP9975003.1"
                     /translation="MVQNQHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI"
     gene            1861..2373
                     /locus_tag="IWM52_002828"
                     /pseudo
     CDS             1861..2373
                     /locus_tag="IWM52_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337518.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            2422..3102
                     /locus_tag="IWM52_002829"
     CDS             2422..3102
                     /locus_tag="IWM52_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9975004.1"
                     /translation="MNHFKGKQFQKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWVQEYSKILYHLWKKKNRQSFYSWKMDETYIKIKGHWHYLYRAIDSDGLTLDI
                     WLRKKRDTQAAYAFLKRLHKQFGQPRVIVTDKAPSISSAFRKLQINGLYTKTEHRTVK
                     YLNNLIEQDHRPIKRRNKFYRSLRTASTTIKGMETIRGIYKKNRRNGTLFGFSVSTEI
                     KVLMGIPA"
BASE COUNT         1065 a          519 c          592 g          978 t
ORIGIN      
        1 ataaaaaagt ggtgcatttt taaattggca caaacaggta acggttattg caggtgtatt
       61 tcttatctat gggtttaaca tggattttat cattaaaatc atgagtattg tccgagagtg
      121 attggtcttg cgtatggtta accctaaagt tatggaaata agacttagaa gcaaacttaa
      181 gagtgtgttg atagtgcatt aaaaattttg tataatagga attgaagtta aattagatgc
      241 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      301 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      361 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      421 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgatta
      481 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      541 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      601 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      661 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      721 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggag
      781 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccatgcgtc
      841 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
      901 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
      961 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1021 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1081 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1141 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1201 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1261 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1321 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1381 aaattcctcg taggcgctcg ggaccccaac atgaggaaat gtttttataa aaaaagagta
     1441 gttaccgttt tttggtaact actcttttcc ttttttaagg tttaaatacc tggaagttta
     1501 ggtgttttgg gtattggtgg tgtaggtggc tgcaagcttt ccaatttttg ttgaattgct
     1561 ttaaattcag gggtttcttg gtgttgattt tgaaccattt tttgctctat tctctccaaa
     1621 gtcggttgaa tttctttccc tgatacttta cgatttaatt ctctttctaa ggtctctttc
     1681 ggactaatgg aaggcgtttc taagcttgaa tagagtttca ctccttctct attgtaaagc
     1741 cttatatcgc taaataagcc cgttttatga agggtttcta aattggtcgg taagtttttg
     1801 acaacaatat catgcgcttg ttttggtggt gccctagctg tcattggatc atctgcatac
     1861 aataagatcc atgaaaggcc atctgctgcc aatgagcgcc ttgagattgg taattgggaa
     1921 gctgatactg tagccggtaa aacaggtagg gctcgtattg taactctagt cgatcgtaga
     1981 tctcgtttct ccctacttgg taaggttgaa aagaaaatct ctaaaaatgt ggttgattgt
     2041 atgatcaatc tactaagaaa tgtaggagaa gataaatgta aaaccgttac acccgataga
     2101 ggaaaagaat tctcccaaca tagtcgaata acgctagaat taaatggcac taaagtgtat
     2161 tttccagatc cacatgctcc atggcaacgt ggtacaaacg aaaatactaa tggtttatta
     2221 cgagaatatt cacccaaagg tgaagatatt tcaggtattt tcgatagtca aattcaagaa
     2281 tgggcgaata aattaaacac gcgaccacgg aaatgtctaa attggaaaac cccttatgaa
     2341 gtatttttgg ttctgttgca aagttcccca taactcctga tttattgtaa aatgtaagaa
     2401 aacgagagtg agggtagaca gatgaatcat ttcaaaggga aacaatttca aaaagatgtg
     2461 attattgtcg ctgttggtta ctacctgcgt tacaatctga gctatcgtga agttcaggaa
     2521 ttactgtatg atcgcggaat aaatgtttgt cacactacga tttatcgttg ggttcaagag
     2581 tacagtaaaa tcctctatca tctttggaag aagaaaaata gacagtcctt ctactcgtgg
     2641 aaaatggacg agacctatat caaaataaaa ggccattggc attatctcta tcgtgcgatt
     2701 gattctgatg gattaacttt agacatttgg ttacgaaaga aacgggatac tcaggcggct
     2761 tatgctttct taaaacggct ccataaacaa tttggacaac ctagagtgat tgtaaccgat
     2821 aaagcaccat cgattagctc tgcattcaga aagctacaga ttaacggttt atataccaag
     2881 acagaacatc ggacagttaa gtatctcaat aatttaattg agcaggacca tcgtccaatt
     2941 aagcgccgta ataaatttta tcgaagttta cgaactgcct caaccacgat taagggcatg
     3001 gagactattc gaggaatata caaaaagaac cgaagaaacg gaacgctctt cggattttcg
     3061 gtgtcgactg aaattaaagt tctgatgggg atcccagcat agtcaaaata ttaaactagg
     3121 acttctatac tcttttgggg aactttgcaa caga
//
LOCUS       DACDYI010000134         3035 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.140, whole genome shotgun sequence.
ACCESSION   DACDYI010000134 DACDYI010000000
VERSION     DACDYI010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.140"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(106..1053)
                     /locus_tag="IWM52_002830"
     CDS             complement(106..1053)
                     /locus_tag="IWM52_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9975005.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            1299..1763
                     /locus_tag="IWM52_002831"
     CDS             1299..1763
                     /locus_tag="IWM52_002831"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAP9975006.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(1825..2868)
                     /locus_tag="IWM52_002832"
     CDS             complement(1825..2868)
                     /locus_tag="IWM52_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAP9975007.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT         1005 a          601 c          581 g          848 t
ORIGIN      
        1 agaagctact tctggaacac catttattcg gaaaaaagga ctgtgacaag acttttgtca
       61 cagtcctttt tcatttaaaa aaatgtttac ttcatttgac gtatattatt ggtgaggctt
      121 caattcattt ggcttcactt tttctgtttc tcttatgata taaacaggac gctgtttcgt
      181 ttctaagaag attttcccaa tatatttccc gatgattcca agacataaca attggattcc
      241 accaatgaac aggaagatcg agaccatgga aggccatcct gatgtaggat caccaaacaa
      301 caatgcccgc aaaataatga acaaaattgc aataccagaa ccgacacaag acaaagcgcc
      361 gataaatgaa gcaatcgtta acggtgcatc cgagaagttc acgataccat caatcgagta
      421 gttgaataat ttccagaagg accaagatgt ttcgccagcc acacgttcgc ggttttcata
      481 agaaatatat tctgttttaa atccgaccca gctaaataat ccttttgaaa atcggttgta
      541 ttctgttaat tctaggacgg catccaccat ttgacgagtc atcaagcgga aatcacgagc
      601 gccatcgacc atttctgtat cgctgatttt attgatcaac tggtagaatt ttttcgcaaa
      661 gaaactgcgt atcgcaggct cgccttcacg agtcgaacgc cgggtgccga cacaatcgat
      721 atcagaagta gtgatccggt tgatcatctc aggaagcagt tcgggcggat cttgcagatc
      781 aacatccatc acggtcacta aatctcctgt agaagcttgc aatccagcgt acagagccgc
      841 ttctttccca aaatttctgg aaaatgagac aaagcggaca taaggataag ctgaggctag
      901 cttcttgatt gctgctaacg taccgtcttt tgatccgtcg tcaataaaca aatattcgaa
      961 ctcatgggca cctttgaatt tttcaacctc atcaaaaaaa agaggaacag ttttttcttc
     1021 attaaaacat gggacaacaa ttgatacttt catcgtttct atcctttcaa tcgtatgttt
     1081 atatatggtt tacattcaaa atttaataaa ccttaaataa cacctttaaa atttgatttt
     1141 acgtaaaaga acaggtattc tttttgtaaa ctaacgtatt tagtttacca tgtttcctcg
     1201 gtttatcaaa cttttaagaa caatttttat tagttggcaa gtagttttgg atagagaaaa
     1261 aggaggaagt tccagtctat tgactaggag caaacaaaat gaaaatacac acacctatcg
     1321 gctttttatg gttagatgca gatgaatccg gattgactgc tgtatctttt cagccaattt
     1381 cagaaagcga atcagcgaat cagcgaattt taacgcgcac acaagaacag tttgaagaat
     1441 attttgcggg aaaaagaaaa gaatttgatg ttcctttatc tataaagaga ggtacgcctt
     1501 ttcagcaaaa tgtctggaaa gctttgtcga ccattcctta tggagaaatc cgaacgtata
     1561 aagaagtagc cgttgctgtt gatagtccga aagctgtgcg agcgattggg caggcaaatc
     1621 gaaccaatcc attgccaatc gttattcctt gtcatcgagt gatcgggcaa aatggaaaac
     1681 tgacaggata tatgggaaac gcaatagatg gggttgaaat caaacgccaa ttgttgttgt
     1741 tagaaggata tctgcaaaat taaaaacagt tgtgattcgg acattgttcc atgcgagaat
     1801 cacaactgtt ttttcatgtt cattctaaac tttgatcagc caaataaata gtaaagccat
     1861 cactgtttgg agaagcccga cagctaatcc ataagataag aagcgtgtac cttctttcat
     1921 gaattttgca aagtctaaac gcagaccaat tgccgctaaa gcagtgatct caaaccagct
     1981 gctgataaaa tgtgccgatt ggtcaataac tacaggtaaa gaaaacaagc tgttgaccaa
     2041 acaaaagatc acaaacccta atacatacca aggaagcggt aattttcgtt ttttctgcgt
     2101 atcagttggc gtttcaaaca gagctggttg tttcttgtgc atgaattttt caaacagata
     2161 gacaacaaca acgagcatca taatacgtag aatcttaaat agcatggcga attcgacaac
     2221 agaattgtca agtaagcttg ccccagcgac tacttgacca acagattgga gtgtaccacc
     2281 caaaagtgca cttttttcaa tgagatttcc accaaacaac tgtaatccaa gaaaagggag
     2341 agtcaacatc atcacagtac ccagtagatt gactagtgtg atgatctgtc ctttttcttc
     2401 ctctttcgca tggatggaag gggcgattga agcaatagca gaagaaccac aaacagcgtt
     2461 tcctccagcc atcatcaaag aagaagtatc gctgaattgc agttttttac caatcaagta
     2521 agcaccgatg atagtgactg acatttgcag taacacaaaa acaattcctt ttacaccaat
     2581 ttgagcaatc gtttgagctg tgacagttgc acctagtaaa aaaacagaga attctaatag
     2641 tttgctttca gcaaattttg tgccgattgc tagaacaggc tgtttaaaaa aagtatttcc
     2701 taataaaata cccaataaaa tagcaatagt cgctccacct aactgtggca gataaatagc
     2761 aagaagcctg ctgaaggcag cgatggcaaa agaaagcagt aaaccgggta agatttcgcg
     2821 gaccgctgaa tcctttacag attttattgg atgtgacaat gatttcattt gatcggttcc
     2881 tttcgtcaat aaatactaaa agcatactcg aacatagtag cactaaacca gtattttgac
     2941 aaaagaacaa gaggtcgtga caaaagtctt gtacgacctc ttgtttcgaa taaacggtgt
     3001 tccagaagta gcttctacgg aaataagccg aaatt
//
LOCUS       DACDYI010000135         3010 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.141, whole genome shotgun sequence.
ACCESSION   DACDYI010000135 DACDYI010000000
VERSION     DACDYI010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.141"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..198
                     /locus_tag="IWM52_002833"
     CDS             <1..198
                     /locus_tag="IWM52_002833"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9975008.1"
                     /translation="IPPAIFFYYSILFDSRFKTLQQNQIVNNSLGTDFTYLDFIGDEG
                     KVANGFSEKIIQFQENRSKLN"
     gene            278..673
                     /locus_tag="IWM52_002834"
     CDS             278..673
                     /locus_tag="IWM52_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9975009.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            998..1615
                     /locus_tag="IWM52_002835"
     CDS             998..1615
                     /locus_tag="IWM52_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9975010.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            1656..1955
                     /locus_tag="IWM52_002836"
     CDS             1656..1955
                     /locus_tag="IWM52_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9975011.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1989..2156
                     /locus_tag="IWM52_002837"
     CDS             1989..2156
                     /locus_tag="IWM52_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9975012.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(2201..2806)
                     /locus_tag="IWM52_002838"
     CDS             complement(2201..2806)
                     /locus_tag="IWM52_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9975013.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTVMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         1060 a          442 c          537 g          971 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aaatagttaa taattcactt gggacagatt ttacttatct tgattttatt
      121 ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa ttattcaatt tcaagaaaat
      181 agaagtaaac ttaattaaga atcgtttact atttaaacag tgtccttttc ctttttaaat
      241 atatacgata aaataagtat atgaattgta aaaggacatg atgattatgg aagaaataga
      301 atggaataat ctgactcaaa ataataaaaa taattctgga tgtggaatta tctttatgtt
      361 tgttatagtt ttgggaatag tagtactttt aataactgtc aagatagtag attactttgc
      421 gaatactcct gaagaacaaa tacagagaaa acttcctgag tttgaaagag taccacctta
      481 tgatccaaga gataattcat ttcaagtaaa aaaattaaaa gagtctccaa atgaaatgtg
      541 gtttgaagtt acagttgaat caagcgactt caaagaaaaa aagaaagaat acaattatat
      601 tgtaaaggca aaagtatcaa aaagatttat ttttaaccct aaattagaaa tatatagtgt
      661 tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa agaagtcaaa ttctgatgtg
      721 aaaaataaaa tataccatgg aagacataaa agaaaaactg gataactagt ttatttaatg
      781 gcagaccgtt aaggcctgcc atttttatgt attttagcgg aataaatata atcatgcggg
      841 agtgtaaact tactgtttca aaaagtatac tttttgacat gccttgattt tctttgttta
      901 tgtatggcat aataaccaca taatacaatt tgaaacgttt ctgatttaat ctgattgttt
      961 tgaaacaatt ttagtgaaaa aagggtagat aaaaacgatg agaaaatttt tttatatccg
     1021 ggtcagtagt tatgatcaat cgatcgaacg acaactgatt tccgcgaagg aattaggaat
     1081 ccctgaagaa tatatatttg tagaaaaagc cagcggcaaa gattttaaac gccctgaata
     1141 tcaattaatg aaacggatgt ttcgaaaagg agatttactc tatctacaat cattagatag
     1201 actaggcaga aataaacaaa tgattttaga agaatggcga gaattaacta aggaaaagca
     1261 gattgatatt tctgtgttag acatgcctct gttagatact gctagttaca agcaactaga
     1321 tgggttagag acgcttatct ctgacttaat tctgcaatta ctcagctata tggccgaaga
     1381 cgagcggaaa cgaattaata gccggcaaag agaaggcgta gaagctgcga aacaaaaggg
     1441 tgtaaaattc ggcagaaaga aaattgcttt acctcctgaa tttcctgcga tctattctga
     1501 ctggaaagca ggaaagataa ctgcagtcta tgctatggat caattgggca tgaaacgaaa
     1561 taccttttat cgacgggtga aagaatatga acataccttg caccactttt cttgatagat
     1621 ataacagcta ttagacggga agggagagtt tgaaaatgga cttaacgaat caaagggtcg
     1681 tttttactgg aacgctgcag caaatgacac gaacccaagc catcggtttg gttcactctt
     1741 taaacggcca ttgtcaatca agtgttactt caaaaacaaa tttcctggtt tgtggtcagt
     1801 attctaagtc gttgtttgat gattctctct atacaaaaaa ggaacaaacc gcgcgggact
     1861 tgattgcgtt aggtcatgag attaccattt tatcagaagt cgaattttat gcgttaatta
     1921 gtcaacagtt gaaagaatgg caacggtatt tataatcgtt agtaaaattt acaaggagga
     1981 aaatttttat gaagcgaaaa tcttatgatc gttggggcat tgaaataact agttttaatc
     2041 gaaatattgc ttttcacgtg gagtgtgact gtggggaatt agcaaaaagt gtttatcaaa
     2101 aaaattactt ttactgtcct aaatgccatc gccgatacgg ggtacatgga acatagaaac
     2161 gaaatagtgc ggtagaaaag cgaatgaatg tagtatattt ctacaaatca ttcgctttct
     2221 ttattttata aatagttggt cgagagatcc cgttttcttt tgcaatctct gcaactggaa
     2281 tgccttgctt taacatgtcc accacggttt taaaaactaa gcgtttttgc ggatctgttg
     2341 agttaataga atactcgatt ggtcgtccct tatatcggcc ggctttttga gccaataata
     2401 ctccttgccg ctgacgttca cgaattttct cacgctcgtt ttgtgcaata taacttagca
     2461 gtgataaaaa catatcgaac ataacggtat ccaggagttc atttccagta ttaaaatcta
     2521 aaaactgtgc atctaatatt tttaacgcta cttttttctg tttcataaaa gcaactgtat
     2581 tttttatatc ttcataatct cttcctaaac gatctagtga tgtaacgatg acgcaatcac
     2641 cttcacggat ataatttagt aatttttgaa actcaggacg ctcagtatct ttaccactgg
     2701 ctttttcttg gaataattta tcaatgttaa gtccttccat cattgccaat tgtcgatcca
     2761 aattttgatc ttgtgaagat actcgaatat agccaatttt tcccataaaa aactcctttt
     2821 taaaagtgat ataatttaca tgtaaaattt acttgtagtt ttactgctat catagcattt
     2881 ttaaaaataa attttatgta gacttaaaat ttacttcatg gagggtttaa atggatgaaa
     2941 tcaaaagtaa caaaaaagca tttggcattt atttaggaga acctagtaca gaacaattaa
     3001 accaattttt
//
LOCUS       DACDYI010000136         2967 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.142, whole genome shotgun sequence.
ACCESSION   DACDYI010000136 DACDYI010000000
VERSION     DACDYI010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.142"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..222
                     /locus_tag="IWM52_002839"
     CDS             <1..222
                     /locus_tag="IWM52_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9975014.1"
                     /translation="FRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKK
                     GNKIAKLEFTFSSLTSDLPKISLRNWLEE"
     gene            complement(359..775)
                     /locus_tag="IWM52_002840"
     CDS             complement(359..775)
                     /locus_tag="IWM52_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9975015.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(778..1062)
                     /locus_tag="IWM52_002841"
     CDS             complement(778..1062)
                     /locus_tag="IWM52_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9975016.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            complement(1762..1857)
                     /locus_tag="IWM52_002842"
     CDS             complement(1762..1857)
                     /locus_tag="IWM52_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP9975017.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            2466..>2967
                     /locus_tag="IWM52_002843"
     CDS             2466..>2967
                     /locus_tag="IWM52_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9975018.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEF"
BASE COUNT          944 a          470 c          561 g          992 t
ORIGIN      
        1 tttaggagct taatggatat cccagaatac tatcaaatgg gtaacattga tcaaaaagta
       61 ttaaatcctg cattacgaga actaaaaaaa tacttcaatg atcttaaagt cacaaaaata
      121 aaagcaaaaa agggcaataa aatcgccaaa ttagaattta ctttttcaag cctaacgtca
      181 gatttgccaa agatttctct gcgtaactgg ttagaagaat aaataaaaaa gaactcgccc
      241 cccttacatt aagcaagtag acaagtccct tacaaccgac taagattgct agtacattct
      301 agcatattgt atggggcttc taaataaaaa acagatagga ataactccta tctgtttttt
      361 atcgttttag aaaatttgtt gggtcgaaat ttctagaagt gactgtttga ctggtttctt
      421 tttgatgatt ctttggtcga tttcgatttg atggatttag tttagcttcg attttttctt
      481 tcacttcttg atatacttta gaatttgtgc caataagaac gcctgtatct tttagatata
      541 aatcagcaaa ttctgcgatg atttcagtca tgatggaaat ttcttttccc atagcattta
      601 gttttgcctg attgctggta gtggtcgtgt tggtatcggg ttcataactc atacataaaa
      661 agcgaatggc ttccgagtag ttattgatat ctggattttt ttcgatgatg gaatctaaaa
      721 cgtcaacatg actagggtac aaagtaacat gtttttggac agaagttttt ctcatggtta
      781 tatcattcct ccgataagca tttcaatgat ttgattattt gtgtagacat actcgcttaa
      841 tatttgagta ttttctgttt ggatagcaat aaatttatcg agcaataaat cgaattggtc
      901 ttttttgtaa gtcgtaagtt gacggtcaaa atcactttcg attaaaactt ttagatattt
      961 atttcgactc catttttctc ctgttttatt cgtgagttct tggcaacgtt tgtctattgt
     1021 tgcaacaaac tttggatcaa tattccgaac taaaatgtcc ataaaaaatc tccttttgca
     1081 attatgtata atttatgtat accatacaaa tactatattt gtatagctgt tttttgccaa
     1141 aatgctatac ataagctacc cacgatggcg aaaaagtgat atcaaatttt tttctgagaa
     1201 acgtgatagg cacagcctat ctacccactt tttttagaaa gtgggtacac gtttccttat
     1261 gctctttaaa attcgggggg gagtgctgca cgtgtaatcg ataaccaaat ttaacagacg
     1321 aaaaattgtt ggaatataaa aatatgacac ccgaaagaaa acgatcgttg gaaaaattaa
     1381 tgcaactagt gaacgaagac ggcggaattt cagacgcagc ttttgacgca tggagcgaat
     1441 ttcatggaga atataattaa gtgggtttaa atggctctaa aatcgcttct aagagttttt
     1501 tagcttggac caatgaaata caccaaaaac taaataaaac gtcttaaatc gaaaaatacg
     1561 agctctcaga gcgactttat ttgtaaatgt tgcatataag aaataattac ggattttaga
     1621 cttttgtagg atatttttga aataaatatt tatttctgtt ataatttgat tgcaaataag
     1681 tgaacacgaa acacacgaaa tccccagaga ggtcggattc tctggggctt tttttgtggg
     1741 ctgagtcgcc ttaattaagt tttacttctt gtttcggtta ttcaaccagt aactgaacaa
     1801 agcgcttaaa agcccaacaa gaagggagca aataaagtta gtgaacacga aacacacctc
     1861 cttccagtcg caagactgtc ggataggcga caatcaatta tacaataaaa aagcaaaaat
     1921 aagcctaagt tttttattgc cttggggttg ttagggcttg ccctgaccgc ctgtaaggcg
     1981 ctttagaacg aaggacatag tttttatcga atcttgtctt agaacggctt agacgagctc
     2041 tcaggacgcc acaaaggagt ccctaatgaa ttatttttgg cgaaggagcc taaaggcgac
     2101 tgctgcaagt aaaatgtgag agcgtagcga tcactccttc ttacagcgac catagggagc
     2161 tagattggca gtttaatatc atgcgcgaac ctgtttattt taaaaagtgg gtgatttttt
     2221 ggcgggaaat tttgctcgcg cgcgcgcgtt tattttattt attttttaaa gattttttag
     2281 aacccttttt agggggcagt tgagccttac tctcccaacg gatacagaag ggtactaaca
     2341 actttttatc tgagtactaa caacttttta tctgagtact aatatccttt tgtataataa
     2401 caaccaacct atttacgagt tggttgttaa aggttattat ttatttgtaa ggggggaaga
     2461 aaggaatgga tgaaaaaacg gaattaacag tcaaatatca aaatgaattg aatttagtac
     2521 cgctgaaaaa tttcaatgct aagcagatgg atttattttt tgcattatgc gctcgtatga
     2581 aagatcgtgg tttgaaaaaa gttagatttt ctttcgagga attaaaagag ttaagtgatt
     2641 ataagatgac tgctacaaaa gcctttgtcg gtgatttaga aaagctctat aaagatatgc
     2701 ttaatcttac ttaccgtact gaagatgagg attctattcg ctactttgtt ttgtttactg
     2761 gttttgaaat caataaaaca aaaaagtttg ttgaagtcag tgttaatccg gacctagaat
     2821 atattattaa tggtttaaca gctgaattta gtagatttga attatctgct ttcacgtcgt
     2881 tgcgttctac gtatgcaaaa actctattcc gactgcttat gcagtaccgt tctactggtt
     2941 attatgtagt tcgtattgaa gagttta
//
LOCUS       DACDYI010000137         2857 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.143, whole genome shotgun sequence.
ACCESSION   DACDYI010000137 DACDYI010000000
VERSION     DACDYI010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.143"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            562..843
                     /locus_tag="IWM52_002844"
     CDS             562..843
                     /locus_tag="IWM52_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9975019.1"
                     /translation="MDILVRKIDSKVIAKIDKRCQELTNKTGKKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLEKFIAIQTKNTQVLNDYVHTNNQLIETLIGGK"
     gene            845..1261
                     /locus_tag="IWM52_002845"
     CDS             845..1261
                     /locus_tag="IWM52_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9975020.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIERNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKT
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1422..1688)
                     /locus_tag="IWM52_002846"
     CDS             complement(1422..1688)
                     /locus_tag="IWM52_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9975021.1"
                     /translation="MAFRFRCDRGGRKMTEKFPYEYYQNLEKLESAIQLVKLDLLDEN
                     LLKYKNITPERKGKLKKMQQLFDEEDGISDVIFDTWSEFHGEYN"
     gene            complement(1646..2620)
                     /locus_tag="IWM52_002847"
     CDS             complement(1646..2620)
                     /locus_tag="IWM52_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP9975022.1"
                     /translation="MGKKRTIVNEWEKLTAPSNRRLNVGESVKMIKMDWSIDRITIVG
                     TLNENIYYHTQNDVLILDFEQLMRVNEGNGHLKAVGNNGWQLVDQHEENIAYIEILKW
                     QEGKGRIDFNPNKISQFLASSMKKFIHDLFLEPHFSRADVACDLIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQEAKRKIVPKEVETWWRLELQ
                     LRRGKATDWYEMVQESLDSFASPHYFPEDVKTLDRVMIKGLLYDHSEWSTISRDLKYR
                     LRKLLKQESQNDELTNHLRETFEESADELKRELDTWLLGLDVTEEEEK"
BASE COUNT          926 a          624 c          397 g          910 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatcattt cacgcttccg ctcttcattt cattcagttc tcggctttca caatctaaca
      121 cgccgtttcg ctaccagtcg ccttacaggc tccattctcg cttctcttac gtgttttacc
      181 gattgtgccg acaactaaac gaaatgaacg ctaaaacgtc ttagaacgca aaatacacgc
      241 ttataaaatc atttcatctt gattatatgc tcatcataat cgttcaattt gacatcattt
      301 ccgtaaactt tttttatttt tataagcaga aaaaagtcgc acatcaacat ttttttaaag
      361 agcataagga aacgtgtacc cactttctaa aaaaagcggg cagataggct gtgcctatca
      421 cgtttctaag aaaaaaattt gatatcactt tttcgccatt gtgactagct tatgtatagt
      481 gtttcagcaa aaaacagcta tacaaatact atattcgtat gatatacata aattatacac
      541 aaatgcgaaa ggagtttttc tatggacatt ttagttcgga agattgattc aaaggttatt
      601 gcaaaaatag acaaacgttg ccaagaactc acaaataaaa ctggcaaaaa atggagtcga
      661 aataaatatc taaaagtttt aatcgaaagt gattttgacc gtcaacttac gacttacaaa
      721 aaagaccaat tcgatttatt actcgaaaaa tttatcgcta ttcagacaaa aaacactcaa
      781 gtactaaacg actatgttca tacaaataat caacttattg aaacacttat tggaggaaaa
      841 tagcatgaga aaaacttctg ttcaaaaaca cgttaccctt tatcccagtc acgtggacgt
      901 tttagattcc attatcgaaa gaaatccaga tatcaacaat tactcggaag ccattcgttt
      961 tttatgtatg agttatgaac ctgataccaa cacgactact accagcaatc aagccaaact
     1021 aaatgctatg ggaaaagaaa tttccatcat gtccgaaatc attgcagaat ttgctgattt
     1081 atatctaaaa gatacaggcg ttcttattgg cacaaattct aaagtgtatc aagaagtgaa
     1141 agaaaaaacc gaagctaaac taaatccgtc gaatcgaaat cgaccaaaga atcatcaaaa
     1201 agaaacgagt caaacagtca cttctagaaa tttcgaccca acaaattttc taaaacgcta
     1261 aaaaataaaa cgagaatttt tttatttctc gttttatttt ttaggaaagc cggtcttaca
     1321 gcaagatttg caagttaccg gtaacttgcg gaaaagaaga agccgctcac gcgacttctt
     1381 ctttttgcat tttctgaaag ctaccacaat tcctaacttc gctaattata ttctccatga
     1441 aattcgctcc atgtatcaaa aatcacatct gaaattccat cttcttcatc aaataattgc
     1501 tgcatttttt tcaatttacc ctttctctct ggcgtaatat ttttgtattt tagtaaattc
     1561 tcatccaaca aatccagctt gaccaactga atagcactct ccaatttttc tagattttga
     1621 taatactcgt atggaaattt ctcagtcatt tttcttcctc ctctgtcaca tctaaaccta
     1681 aaagccatgt atccaattct ctttttaatt cgtctgcaga ctcctcaaat gtttctcgta
     1741 aatgattcgt caattcatca ttttgacttt cttgcttcag caatttcctt agccgatatt
     1801 tcaagtcacg actaatagtt gaccactcag aatggtcata caacaacccc tttatcatca
     1861 ctctatccaa cgtttttaca tcttcaggaa aataatgcgg actagcaaaa ctatccaaac
     1921 tttcttgaac catctcatac caatcagtcg cttttccacg gcgcaattgt aactccaaac
     1981 gccaccacgt ctcgacttct ttaggtacaa tttttcgctt tgcttcctgt tccaacttct
     2041 tgttgtacat ccgaatttgg cgttcgctgg cacgactacc ccaataagcc gtttctagct
     2101 taccagagcg tccataaatt ggtttgaagg acacagggtc aactacacga tattgtgtaa
     2161 taaactcatc aggcacatca ataagatcgc aggcaacatc cgctcgtgaa aaatggggct
     2221 ctaaaaataa atcatgaata aattttttca tagagcttgc taaaaattga ctaattttat
     2281 tggggttaaa gtcaatccgc cctttaccct cttgccattt caaaatttcg atataggcaa
     2341 tattttcttc gtgttgatct accaattgcc aaccattatt cccaacagct tttaaatgac
     2401 cattaccttc gttcactcgc attaattgct caaaatctaa aatcaacaca tcattttgag
     2461 tgtgataata aatattttca ttcaaagttc cgacaatcgt aattcggtca atcgaccaat
     2521 ccatttttat catttttacg ctctcaccga catttaaccg cctgttagag ggggctgtta
     2581 atttttccca ctcattcaca attgttcttt ttttacccaa attttgttcc tcctcaaaga
     2641 aaaaaagaga tttttggaaa gaatttccag agttcacgta actctaaaaa tccacatcca
     2701 aaaatctcta gcttgaagaa acctaaaaat attcgtataa tgaacttgag ttacggaact
     2761 aggctagtag tttccgtaaa ggtcgagagt gttagcgcac tgctcgacta tttttttgct
     2821 cttttttctc gttttctttg attatagcac tcctctt
//
LOCUS       DACDYI010000138         2821 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.144, whole genome shotgun sequence.
ACCESSION   DACDYI010000138 DACDYI010000000
VERSION     DACDYI010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.144"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            95..1054
                     /locus_tag="IWM52_002848"
     CDS             95..1054
                     /locus_tag="IWM52_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS1252 family transposase"
                     /protein_id="HAP9975023.1"
                     /translation="MTYTHLTTDELVMIESYYHQNIKIVKIAEYLKRSRTPIYNVINF
                     LKEGHTALEYYQQYKENKKRCGRRNIVLPKKQQSYIKEKIAQGWTPDVIIGRKEKPIS
                     CSVRTLYRQFKEKVFDESTLPMKGKRKPNGHQERRGKQAFKRNIVERETDYPTFKEEF
                     GHIEGDTIVGIHHKSAVITLVERLSKIIITLKPEGRKAIDIENTLNHWFGSIPRHLFK
                     SITFDCGKEFSNWKALSNQHDIAIYFADPGTPSQRALNENSNGLLRKDGLPKNMDFNQ
                     VDQAFISSVANKRNNIPRKSLDYQTPLEVFMSYMNEDILSSLI"
     gene            complement(1487..2059)
                     /locus_tag="IWM52_002849"
     CDS             complement(1487..2059)
                     /locus_tag="IWM52_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9975024.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2075..2680)
                     /locus_tag="IWM52_002850"
     CDS             complement(2075..2680)
                     /locus_tag="IWM52_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAP9975025.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          888 a          579 c          469 g          885 t
ORIGIN      
        1 gccgattgta aaattaagct agacaaataa ataagccatt cgtgctacac tcaaaatgta
       61 attccgacta aagaaaacat aggagtgatc acgaatgacc tacacccatc ttaccacgga
      121 tgaactggtg atgatagagt cttactacca ccaaaacatc aaaattgtga aaattgctga
      181 gtacctgaaa cgatctcgaa caccgattta taacgtcatt aatttcttga aagaaggcca
      241 tacggccctc gaatactacc aacagtataa agaaaataag aaacggtgtg gcagacgaaa
      301 catcgtcttg ccaaagaaac aacagtccta cattaaagaa aaaattgccc aaggctggac
      361 gccagacgtt atcattgggc gtaaagagaa accaatcagc tgttccgtaa gaacccttta
      421 tcgccagttc aaggaaaagg tgttcgatga atcgactttg cccatgaagg gtaagcggaa
      481 accaaatgga catcaagaac gccgaggaaa acaggctttt aaacggaata tcgttgaaag
      541 agaaactgat tacccgacat ttaaagaaga gttcggccat atcgaaggcg ataccattgt
      601 aggcattcat cataagagtg ctgtcataac tttggtagaa cgtctatcga aaatcatcat
      661 caccttgaag cccgaggggc gcaaggcgat cgatattgag aatactctga accactggtt
      721 tggatcgatt cccagacatt tatttaaatc cattaccttt gattgtggga aagaattctc
      781 taattggaag gctttaagta accaacatga catcgctatt tatttcgctg atccagggac
      841 tccttcgcaa cgagcactga atgaaaattc caatggcttg ctgcgaaaag atggtctgcc
      901 aaaaaacatg gattttaatc aagtcgacca agcttttatc tcttcagtgg cgaataagag
      961 aaacaatatt ccacgaaagt ccttggatta tcaaacaccc ctggaagtat ttatgagtta
     1021 catgaatgaa gatattttgt ctagcttaat ttgacaaatc aaattattta aaactttgca
     1081 acagaacctt agcagcagtg ctgattttta tgatagttat ttggttctta aatgaaagat
     1141 ctaaagacaa tcaacaccgt agggatatgg agataaaatc aatagagtca atagattatt
     1201 tatcgataat aacactaaaa aagccacgtt cctaaacgtg acttacgctg aacgatatgt
     1261 gttgtttggt agacgaacaa atcgttcatt ataaatatat gatggtatcc taatatagta
     1321 agcgtggcta gcataccttt aattacttta ccttttacag ttaccataca cattttttga
     1381 gcagaaatca agtaaataaa aaaatattat atttcacaac atgctaatag actcatatct
     1441 agaacgcatt aagtatttac ctgtctggtt cgctgagact tcttttttaa ctttcttcaa
     1501 ttttcttctt tattctaaaa agcgtactgc gactgaaacc ggttatagct tcaacttcct
     1561 tatacgattt acctgaagta agcagttcat atgcgtgcct cttcttcggt gtgattgttg
     1621 ctttcggacg tccttctctg aaattaggat tattctttct tgcaaacatt tttccttctt
     1681 gggtacgttc cacaatcata tcacgttcca tttctgctac actcaacaac gtccgtgtca
     1741 ccattcgccc catggacgta ttttcaatcg ttcctagatt caaaaccttg atcgttacgt
     1801 tatcatcgag taaaggttca atgatctcta atgcttctct cgtgttacga gcaaatcgat
     1861 caagcttcgt aacaactatt tcatctccag gcttcagacc agccaacagc tgattgaatt
     1921 ttggccgatc ccgttttgta cccgtaaatt tttctgaaaa aatattcttc ttctctacgc
     1981 cgttctcaag caactgttga atttgttctt ttgtactttg agaagcggtt gagacacgtg
     2041 catatccata tttcgtcatt tggtccacct acttttacaa atcatctatc gtatactcag
     2101 aatacccttc atagaaatag ctgacatcga ttcctttcat atataactcg cgatcgtcaa
     2161 ttttatcagt cagagcattg gaaattagat agcgtatttc caaatcgttt atgggacttc
     2221 gctccattgc agaaagatag tctgctttat cgattaagtt ccaatctaca actttttgca
     2281 attcttcctt taaaatgaga tccagccaaa ttctagtact tcggccattt ccttctcgaa
     2341 atgggtgagc gatattcatc tccacatatt tcttgataat ctcttcaaaa gaagtttgtg
     2401 gcatctgatc aatatgctgt aacgaatgtt ctaaatacat aactggtgca aatcgaaaat
     2461 ttccttttgc aatattgacc gatcggattt ttccggcaaa atcgtaaata tccgaaaata
     2521 gaaattcatg aatttcagat aaacctttaa atgtaccaac ttcgatttca tttatttttc
     2581 cacaatcgta caattctttc gctttttgtt tactgagttt ttcttcaacc ttagccaact
     2641 caacttgatt ggttaatccc aatttattct ctaaaatcat gcgaaatctc cttaacttta
     2701 tgacataagt ttatgaaaca ctctcaaccc cattatatcg cgattttgtt tatgtgtcaa
     2761 aaactattaa gtttctgacc cccgatagat ttttcaagtg tctctatttt gcacagtcta
     2821 g
//
LOCUS       DACDYI010000139         2780 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.145, whole genome shotgun sequence.
ACCESSION   DACDYI010000139 DACDYI010000000
VERSION     DACDYI010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.145"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..197
                     /locus_tag="IWM52_002851"
     CDS             <1..197
                     /locus_tag="IWM52_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9975026.1"
                     /translation="WFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLIIAIIAVGLV
                     FNAAGVKDILLELFNRIIGA"
     gene            314..811
                     /locus_tag="IWM52_002852"
     CDS             314..811
                     /locus_tag="IWM52_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP9975027.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            786..1292
                     /locus_tag="IWM52_002853"
     CDS             786..1292
                     /locus_tag="IWM52_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9975028.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            1276..>2780
                     /locus_tag="IWM52_002854"
     CDS             1276..>2780
                     /locus_tag="IWM52_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAP9975029.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKA"
BASE COUNT          839 a          515 c          600 g          826 t
ORIGIN      
        1 actggtttct atcaaacgct caatcactgg tgcttttagc aatcgttgtg attggcttgt
       61 atcttggctt caagcgtgag tttagcaaac tgattggctt tttaattatt gcgattattg
      121 cggttggctt agtcttcaac gctgctggag taaaagacat tttactagag ctattcaatc
      181 gcattattgg tgcttaaata aaaccgttct tttgtggaat ataagtggtt ttcttatgtt
      241 ccgcaaagga atggtacacc aaacgaagtg cggtagggat ttttgaatct ctacaaagaa
      301 aggacgtgaa tatatggacg atatgcaagt ctatattgcg aatttaggca aatacaatga
      361 gggcgaattg gtcggtgcgt ggtttacctt tcccattgac tttgaggaag tcaaagagaa
      421 aatcggcttg aatgatgaat atgaggaata cgccattcat gactacgagt taccctttac
      481 ggttgacgaa tacacttcca ttggcgaact caatcgacta tgggaaatgg tatcggaatt
      541 acccgaagaa ttacaatcgg agctatctgc tctgctcact catttttcaa gcattgaaga
      601 actaagcgaa catcaagagg atattatcat tcattccgat tgtgatgata tgtatgacgt
      661 ggcacgctac tacattgaag aaacgggtgc tttaggcgaa gtaccagcta gtcttcaaaa
      721 ctatattgat tatcaagcct atggtcggga tttagacctt tcaggaacgt ttatctcaac
      781 caatcatggg atttttgaaa tcgtctatta aatctgtcgg tacattacta ctggcagatt
      841 ttctatttta cggggtggct caatcagcta cccctatttt ttatgaaagg attgattaca
      901 tgaagaaaat acgaagctat accagtatct ggtctgtgga aaaggtactg tattctatca
      961 atgattttag acttccgttt cccataacct ttacgcaaat gacatggttt gtcgtgtcac
     1021 tctttgcagt gatgatactt ggcaacttgc cccctctttc catgatagag ggagcatttc
     1081 tcaaatactt tgggattcct gtggctttca catggtttat gtctacaaaa acttttgatg
     1141 gtaaaaagcc ttatggattt ttgaagtctg tcattgctta tgcactgcga ccaaagctga
     1201 cctatgcagg aaaaaaagta acgcttggca gaaaccagcc acaagaagcc attacagcag
     1261 ttaggagtga attttatggc atatccaatt aaatacattg aaaacaatct cgtctggaat
     1321 aaagacgggg aatgttatgc ttactatgag cttgttcctt acaattactc atttctaagt
     1381 ccagaacaga aaatacaagt gcatgattct ttcagacagc ttatcgcaca aaatcgtgat
     1441 ggcaaaattc atgctttaca aatcagtaca gaatccagca tacgttctgc acaagagcgt
     1501 tccaaaaatg aagtcactgg caagctcaaa gcggttgcct atgacaaaat cgaccaacag
     1561 acagacgctt taatatccat gattggcgaa aatcaagtga actaccgttt ctttatcggc
     1621 tttaagttgc ttctcaacga tcaggagttt tctatgaaaa gtcttaccgt tgaagcaaaa
     1681 aatgctttgt ctgattttgt ctatgatgtg aaccataagc tgatgggcga ttttgttagt
     1741 atgagtaatg atgaaatcct gcgttttcag aagatggaaa agctcttaga aaataaaatc
     1801 tctcgtcgtt tcaaaatccg caggttagat aaggacgact tcggctatct gattgaacac
     1861 ctttacggac agacaggcac tgcctatgaa gagtatgagt accatctatc aaagaaaaag
     1921 ctggataatg aaacgctgat taaatactat gacttgatta agcctactcg ctgtttggtg
     1981 gaagaaaaac agcgatattt gaaaatccag caggaagatg aaaccgtcta tgtagcttac
     2041 tttaccatta acagcattgt cggagaactg gacttcccgt cctctgaaat cttctactac
     2101 cagcaacagc aatttacatt cccgattgat acgtcaatga atgtggaaat tgtagcgaat
     2161 cgtaaagccc tatctactgt ccgcaataaa aagaaagaac tgaaagactt ggataaccac
     2221 gcttggcaaa gtgataatga aaccagctcc aatgtggcgg aagctctgga aagtgtgaat
     2281 gagctggaaa ccaatttaga ccaaagcaag gaatctatgt acaagctgtc ttatgtggta
     2341 agggtatcag caaatgatct tgacgaactc aaacgtcgtt gtaatgaagt gaaagatttt
     2401 tatgacgatt taagcgtaaa actggtacga ccatttgggg atatgctcgg cttacatgaa
     2461 gaatttttac ctgccagcaa gcgttatatg aatgattata ttcaatacgt gacctctgat
     2521 ttcctcgctg gtttaggttt tggtgctact caaatgctgg gggaaaatga ggggatttat
     2581 gttggctaca gcttagatac tggacgcaat gtctatctga aacctgctct tgccagtcaa
     2641 ggggttaagg gttcagtaac caatgcgtta gcgtcggctt ttgttggttc gctgggtggt
     2701 ggtaaatcct ttgcgaataa ccttatcgtc tattatgcgg tgctttatgg ggcacaagca
     2761 gtgattgtag acccaaaagc
//
LOCUS       DACDYI010000140         2650 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.146, whole genome shotgun sequence.
ACCESSION   DACDYI010000140 DACDYI010000000
VERSION     DACDYI010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.146"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            36..197
                     /locus_tag="IWM52_002855"
     CDS             36..197
                     /locus_tag="IWM52_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002392227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9975030.1"
                     /translation="MAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLL
                     SLFNRIVGA"
     gene            210..713
                     /locus_tag="IWM52_002856"
     CDS             210..713
                     /locus_tag="IWM52_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP9975031.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            773..1162
                     /locus_tag="IWM52_002857"
     CDS             773..1162
                     /locus_tag="IWM52_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9975032.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            1149..>2650
                     /locus_tag="IWM52_002858"
     CDS             1149..>2650
                     /locus_tag="IWM52_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAP9975033.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKG"
BASE COUNT          788 a          439 c          610 g          813 t
ORIGIN      
        1 aatggttttt aaccaatgcc caatccttag tcttactggc aattgtggtc attggtttgt
       61 ttttaggatt taagcgggag ttttccaaac tgattggctt tttggtgatt gctttgattg
      121 cggtcggatt ggtctttaat gcttctgggg tgaaggatgt cttactcagt ttgtttaacc
      181 gaattgtcgg tgcgtaaggg ggaagcacga tggaacagat gcgtgtttat attgcgaacc
      241 tcgggaaata taacgaaggt gaattggtgg gggcgtggtt taccccacct gtcgattttg
      301 atgaagtgaa agaacgaatt gggttaaatg acgaatacga ggaatatgca attcatgatt
      361 atgaacttcc ctttgagatt gatgagtata cgccaattga ggaaattaat cggttgtgtg
      421 gcttagcgga ggaacttgaa ggaacgccag ttggtgaagt tgcttctgag attcaacatg
      481 ctttcttcaa ttcttttgag gagatggtag agcatgtgga tgatattatc tattatccag
      541 attgcgatga tatgagtgat gtagcatatt tcttgattgt agaagctggt gattttggag
      601 aagtaccaga acacttgaag acgtatattg attttgatgc gtgtggtaga gacttggaga
      661 ttggaggcaa ttatttggta acgagtcgag gtgtttttga gtatccatat taacaaaatt
      721 attgaagggt agttgatttc taaactatcc ttctttttaa aggaatgatt tgatgaagaa
      781 aataaaaagc tacaccagta tttgggcagt tgaaaaggtc atttatgcaa ttaatgactt
      841 tcaactgcct tttcctgtga cttttaacca gatggcttgg tttgttttat ccttgctgtt
      901 tgtcatcgta tttgcccatg tgcctccact atctatgata gagggggcct ttttaaaata
      961 cttaggtata ccggtggcag ttacttggtt catgtcgcaa aaaacgtttg acggtaagaa
     1021 accattggga tttttacgat cttttattag ctatcactta cgatttaagg tcactttcgc
     1081 aggaaagaaa gtcaaagaac agaagaaacg atgggacgaa cccattactc tggtaaggag
     1141 tgtgaactat gtacccgatt aagtatattg aaaataatct tgtctttaat caagagggag
     1201 aatgttttgc ctactatgag ttaatacctt acaattactc ctttttgtca cctgaacaga
     1261 aatatcaagt ccacgataac tttcgtcaat tgattgcgca aaatcgtgaa ggcaagattc
     1321 atgccttaca aattgccaca gaaagtagta ttcgtgccac gcaagaacga tcaaaaaagg
     1381 aaattaccgg gcgattaaca gaagtagcga aacaacgaat tgatttacag acagaggcat
     1441 tagtatccat gattggcgat agtcagattg attatcgctt ttttattggc ttcaaactga
     1501 ttgcgacaga tgaagaggtc aatctaaaga atctcaagaa atctttcttc tccggctttc
     1561 aagaatttgt ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg
     1621 aagaaattcg ccgatatacc aagcttgaaa aactaatgga aagtaaattg gctcgaagat
     1681 ttaaagtcag acgagtcaca ccgagtgatc tgatgtattt gattgaacat atctatggcg
     1741 aaaagggtac accatttgaa gaatatgaat ttcagctacc aaagaaaaag ctgaagtcgg
     1801 aaactttggt gaaacgttac gacttattac gtcccagtcg ttgtttgatt gaagaaaagc
     1861 cccggtactt atgcatggaa catgagaatc atgaatcata tgtagcgtac ttaacaatta
     1921 atacaattgt gggggaaatg gagtttccct cctcagaact tttctattac cagcaacaac
     1981 aatttacatt cccaattgac actagtatga atgtggagat tgtgacgaac aaaaaggcac
     2041 ttgctaccgt tcgcaataag aaaaaggaat taaaagactt ggacaaccat gcctatcaat
     2101 ctgacaatga aacaaattcc aatgtcttag atgcattgga ttccgtagat gaattggaaa
     2161 ctaccttgga tcagtccaaa gaatccatgt acaaactgag ctatgtggtt cgagtaagtg
     2221 ccgaatcagt agatgaacta aagcgccggt gtgatgaagt gctagatttt tatgatgata
     2281 ccaatgtaaa actagtccgg ccttttggcg atatgatggg gctacatgaa gaattcttac
     2341 cttctagtaa gcgatacatg aatgattata tccagtatgt tacgtctgat tttctcgccg
     2401 gacttggttt tggcgccact cagatgttag gggagttgga aggcatttac tttggttata
     2461 acgtagatac ggggcgcaat gtttacctga aaccggcatt ggcctcacaa ggggtcaagg
     2521 gttcagttac caatgcgcta gcagcggcat tcctcggttc actcggtggc ggaaagtcct
     2581 ttagtaacaa tctcttagtt tattacgccg tcctttttgg tggacaagct gtgattgtcg
     2641 atccaaaagg
//
LOCUS       DACDYI010000141         2643 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.147, whole genome shotgun sequence.
ACCESSION   DACDYI010000141 DACDYI010000000
VERSION     DACDYI010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.147"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            724..2550
                     /gene="ltrA"
                     /locus_tag="IWM52_002859"
     CDS             724..2550
                     /gene="ltrA"
                     /locus_tag="IWM52_002859"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP9975034.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
BASE COUNT          951 a          461 c          584 g          647 t
ORIGIN      
        1 aaaagagtag gcacagcact tcattaaatc aatatttaat gtgtgttgga gaactaaaca
       61 cacgaatagt cgaatgacgg ggtaacgccc tgaaaggcta ctttaatact ctgactggca
      121 gaaacttgag taaacaaggc tctgtcagaa gctcggtgaa gtcggcagac agacaccgta
      181 acagataatg ctgacgaaag attcccagag gtgggcgttg tgtcctatat gccggaagtc
      241 tataaaatat ctatggttag aatgtagatg agaggtctac tgacgaatcc ttgaatgtac
      301 aggtctatat cggggctgga tagaaatgtc cagtgacaca aacagtgtcg ctactgtggg
      361 tgagtaagac tccgcattta tgaaaatcca tataacgtta aaggcgttat caaggtagca
      421 ggcttataga ggaaacctaa ggatatatgt acagatagtg tgtttggaac gttgaaagct
      481 gacaacatgg agattttact gtctgtgaag tgttcacatc agaaagttat tgtgagatta
      541 gcaatgatat aaggtgtgtt aatgtcagtg agagtggtgg cacagtacct atgaaaccgt
      601 gataacaagc ggtggaggga tagccactag tcatatgaag aatagaatta ctaagaaaaa
      661 tggtatgggt tcgagtaaga ctaagagatg tacttgctcc tgcaaacaag gagggtacag
      721 accatgctaa agaaaaacaa gctccgctat aacgagtatt atgatatgca acatatttat
      781 gacgagttat acgcacagag caagaacggt aataactttt ataagttact tgaaattatt
      841 ggttcggaac aaaatatttg tttagcttac cgtaatctga aatcaaacag tggcagtaaa
      901 acggctggaa cggatggaat gacgatagac gatataaaac aactcagcaa tgctgagatt
      961 gttgctaccg ttcgggaaag ccttagcaac taccgcccca aatcagtcag acgtgttttc
     1021 attccaaaag ctgggagcga taaaatgcgt ccattgggga ttccgtgtat ctgggacagg
     1081 ctggtacaac aatgtatcct gcaagtccta gagccgatat gcgaaccaaa gttccataat
     1141 cattcctatg gattccgtgc gaatcgaagt gcccaccatg cagtcagcag agtaacgaca
     1201 ctgataaatt tatcaaaata tcattactgt gttgacgtag acattaaagg atttttcgat
     1261 aatgtcaatc atggaaaact attaaaacaa atctggacac ttggtattcg ggataaaaga
     1321 ttgatttgca ttatcagcaa aatgctgaaa gccgaaattg acggcgaggg tgtcccagaa
     1381 aaaggaacgc cacaaggcgg tctgttaagt ccactgctct ccctcatagt ccttaatgaa
     1441 ttagactggt gggtaagcag tcagtgggaa acattccagc cgaagaaccg cagtaaaaat
     1501 gggtggcttc aatacgcaaa gaaatatacc aaactgaaaa gcggttttat tgtacgttac
     1561 gctgatgatt ttaaaatcat gtgttcaacc tatggagaag cacaaagatt ttaccacagt
     1621 accgtagatt ttcttaataa aaggctgaaa ttagaaatca gtccagagaa atcaaaggta
     1681 gtaaatctga agaaaaattc atcagatttt ctggggttca aaatcaaagt cattccaaaa
     1741 ggaagaacga aacacggtta tgttgccaaa acagatatga accaaaaagc attaaagaaa
     1801 gcgaaaacaa atctaaagtt aaaggtaaaa gatatagcca gacatacgac tggcttcaat
     1861 atcagcagat ataatctgac agttatcggt atgcagaatt actactgcat agcaactaac
     1921 gtatataaca acctgacaga agtaagttac gctctcttac cgaccatacg aatccgttta
     1981 aggaacattg caaaatcagt tccatttgaa agtacaagtt cagaatttca gagcaggaca
     2041 aaaggaatca gaccaaaaac aaaaatagtg atgattgcgg ataatccgct attgccgatt
     2101 caaggagtac aacacaaaaa tcctatgaat ttctcgcaag atatttgtaa ttttacaaag
     2161 caagggagaa acaaggtgca tgaggacgtt gtagttgtaa caaaagaaga aatccgagcg
     2221 ttgttggaaa atgaaaaccc tgccgatagc gtagagttta acgacaatcg tatttcggca
     2281 tatatcgcac aacaaggcaa ctgctatgta atgaatcgtc gaggaactcc gtccactctg
     2341 atatgtattc acaaatcaga caaaggagat aaccttgaca gatattctaa tctggcattt
     2401 gtggaaattc ccattgcaaa ggcaatccta acagaatcag cagacgaagc aaaatcactg
     2461 ttaaaaggtt atgttcttaa tagtcagcag aagaaaaaac tgaacagaat acggacgaat
     2521 tacggatacc agacaataac tgggaaataa cttataaatc tatttggata tgatggaacg
     2581 ccgtatgaag cgaaagtttc atgtacggtg tgaagcgggg gaaaacttgg agataacatc
     2641 aaa
//
LOCUS       DACDYI010000142         2630 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.148, whole genome shotgun sequence.
ACCESSION   DACDYI010000142 DACDYI010000000
VERSION     DACDYI010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.148"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(61..2424)
                     /locus_tag="IWM52_002860"
     CDS             complement(61..2424)
                     /locus_tag="IWM52_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HAP9975035.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
BASE COUNT          700 a          553 c          483 g          894 t
ORIGIN      
        1 cttaatttga caaataatac aatgtaaaaa attctgtttt gcgggatcga ttttatcatt
       61 ttattggtca agatttttcc atttccaatc caatacttct ggcagatcaa taccattttc
      121 acgaatatag ttttgatgtt cttgtaattt ctgatccatt ttttccgcaa aatcttctgc
      181 ttgacttcct ttgattttta aagctgcgtc ttttgccaaa tgataacgat caagttcatt
      241 tactacacgc atgtcaaatg gtgtcgtgat atctccattt tctttgtagc catgaatcat
      301 taaatgatgg ttatgacgat caaagaaaat cgtgcggacc aatgtttcat aaccgtggaa
      361 agcaaataag atcggtttgt cttttgtaaa atattgatca aattcttcat cgctcaaccc
      421 acgcggatct tgacttggat gacgtaattt caataaatct acgacattga tgaaccgaat
      481 ttttagtttt gggaattctt ccagcagcaa agttacagct gctaatgctt ctaagttaga
      541 ttccgttcct gcagcagcaa tgaccaattc aggttcctcc tcttttgctg tcgaagccca
      601 atcaatgatt ttcaagcctt cattcactaa gacggctgct tcttctgctg agtaaaattg
      661 agcacgcgga tgtttggaag agatgatcaa attgattttt tctgacgagc ggaatgcttt
      721 atccatgact gcgagcaatg tatttgcatc cgcaggcaga tattcacgga tgaactccgc
      781 cttcttttct gccaaatgtg tcaatattcc tggatcttgg tggctatatc cattgtgatc
      841 ttgctggaag actgttgagc ttgcaatgag attcagcgaa ggataatctt ttcgccaaga
      901 aaggtcatgt gatttccgta tccatttgaa gtgctgagtc aacatagagt caaccactcg
      961 caagaatgat tcgtaacttg caaaaaaacc atgccgtcca gtcagaacgt acccttctaa
     1021 gaacccttca tcttggtgtt ccgataattg actgtcgatg acgcgaccag atggcgaaag
     1081 ccattcatca ttttctggtt cgattttttc catccattgg cgatttgtcg ttttgaaaac
     1141 ttgattcaat cggtttgatt ttgtttcatc tggtccaaat atacggaaat tttctggatt
     1201 tttctcaatg atctcgcgaa cgaatttacc aagttcggtc atatcttctg ctttaacgga
     1261 gcctggtttt tcaaattgca aagtatattt tttccaatct ggcataataa gcggtcttgg
     1321 atcgattcca ccattggtga taggattttt tgccattctt tggtcaccag taggcgcaat
     1381 ttctctgatt tcttctaaaa tacgtccttg tgcatcaaat aatttttctg gttcatagga
     1441 tttcagccaa cggagtaaag catctgcatg atccatatgt tcttgatcta caggaatcgg
     1501 tacttggtgt gctcggaagg ttccttcaat tggttccccg tcccattctt ttggaccggt
     1561 ccatcctttt ggtgtgcgta cgatcaatac tggccatttt ggcatcacga catctgttgc
     1621 tgtcttcagg cgagcttctt tttggatgga tttgattttc tcgattgctt gatccatttt
     1681 ttcagccata acaggattta atttttctgg atcatttcct tcaataaaga aaggctccca
     1741 gccaagtccg ttaaagtaat ttgctaattc tccatctgat ttacgtgcca agatcgttgg
     1801 gttggctatt tttgcaccat ttaaatgaag gatcggtaaa acagctccat ctatcactgg
     1861 attgataaag acatttgaga accagcttcc agctaaagga ccggtttctg cttcaccatc
     1921 accaatcacg acagcagaga ttacttcagg attgtctaat acagctccga ctgcatgtga
     1981 aagagagtat ccaagctctc cgccttcatg gatagaacct ggtgtttctg gtgctgcatg
     2041 agaagcaatg cctccaggaa atgaaaatcg cttgaataat ttttgcattc ctgtctcatc
     2101 ttccgttact tctggataga tctctgtgta acttccatct aaataagcat tcgataccat
     2161 gacttgtccg ccgtgtcctg gcccttcgat ataaaacatg ttcaaatcat atttattgat
     2221 gactcggttc aaatggacat aaataaaatt ttggccagga atcgttcccc aatgaccgat
     2281 tggatgtttt ttgacatctt ctggtttcag tgtacgtctc agtaaggggt tatctttaag
     2341 ataaagttgc ccaacagaaa gataattagc agcacgccac caagccgtca ttttatcaaa
     2401 gtattcttta ctagaataat ccatacataa cactcctttt cattattact cttattctag
     2461 cgcagatact cccaattgaa acctaataag cacttctgtt catttgtttg gattttatat
     2521 ctagccaaat gaacaggtat aaaaaaccac gaaatttttc ctttcgctga cgttttttac
     2581 attgatccaa aaaacttgtc gttatcacaa tggctgttcc gcagttcatt
//
LOCUS       DACDYI010000143         2551 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.149, whole genome shotgun sequence.
ACCESSION   DACDYI010000143 DACDYI010000000
VERSION     DACDYI010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.149"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..135
                     /locus_tag="IWM52_002861"
     CDS             <1..135
                     /locus_tag="IWM52_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9975036.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
     gene            360..2222
                     /locus_tag="IWM52_002862"
     CDS             360..2222
                     /locus_tag="IWM52_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9975037.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2222..>2551
                     /locus_tag="IWM52_002863"
     CDS             2222..>2551
                     /locus_tag="IWM52_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9975038.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          921 a          383 c          485 g          762 t
ORIGIN      
        1 ttcaaccaag tcaatcaagg attcatctca tccgttgcgt ccaaaagaaa ccatatccct
       61 agaaaatcac taaattacca aacaccatta gaagtttttt tgagttacgt aaatggaaag
      121 ttttgtctcg cttaatttga caaataatat ttaaaaaatt tgaaaaagcg tttgattatt
      181 tatattacga cttagtgcaa taaaaaacgc tttttaacag tacatgtagt cgtctttata
      241 atagataaac tctgtaatat tagttaaaaa gctataagtt gcacttagtt aagtgcaaac
      301 atttcatatt gtcgttagtt tagaaaacgc ttatattagc aatgtaagga gagatgataa
      361 tgcagcagct aacaaaaaga gaaaggcagc ttttgattta cttggtggaa atgtctgaac
      421 cggtaacaat caaaaagttg gctgaaaaag aaaatgttag tgttcggacg ataaaatacg
      481 atctagatga tataagagag tggctgaatg agcggaagca agtcctatat agcaaacgta
      541 gtcagggaat ttggatgaat attagtagct ctgatcgaat caaattaaaa agcgaattga
      601 tggatgttaa tcgttttgaa atatttgcag atcaaagtct ccgaatcaaa cgtttactaa
      661 ttatgctttt agtaacaaaa gaagctatta ctggaatgaa aatggccaac gcattagatg
      721 tcagcaaaga tacgatcatg aatgatttag atgtgctgga aaataatttt cttaaggaag
      781 gattgacctt gaatcgtcag gcgcgaaagg gtttttggat cataggagaa gaacgtctag
      841 ttcgattaac gattgaagag attcttcaaa aagagttcac ggattatgac atttataaat
      901 taatgtcatt gttattgaat ggcggtgaga ctgaatactt tgaaatgtat tctgctactg
      961 ctacacctat ccaagaggta tttaatcaag taatcattcg aatgcgacac ttactggaat
     1021 ttgaaaatct agagaaactg aattacgcgg aattactaaa tattctgatt cgagtgacaa
     1081 ttgctaccgt tcgcttacgc aaagaggcta cgattggtcg ttatcaattg gttgctgaac
     1141 aagaattaga caaaaacgat ctttcttatc gcgtcatgca gcaggtcttt gattattatc
     1201 agttgccact gttcgaggat gaatatcatt atatctatag tgatacattc gacacgattc
     1261 accagcaaga cgtcatgaaa ctagttgaga atgtgattta taaagtaagt aaagcaatca
     1321 accattcttt ttatcaagat ccacaattgt tgacgaatct gtatgctcat ttatccatgc
     1381 gattatcgag aaagaaaaaa tttgtcaatg aatataatcc ttttaaagaa gatatcaagc
     1441 agaaataccc ggaattattt gatgcgatca agcaagcagt tcgacaagaa ataagtgaac
     1501 aaaatattct gatcaatgat tctttcgtgg catatattgc cttacacttt ttggtttcct
     1561 acgaaagaga agaagatcta cgaagcgtta gagccgtcta catttgttca acagggttgg
     1621 gtgtgactag tctgatcaag caaaagatca atgaagaatt aaataatatc actatcgtgg
     1681 catttgcttc agtattgaat gcgcaagaaa tgattgctac caaagatcct gatttggtga
     1741 ttagtatttt tccaattaat ggcatcgatc gtcctttcat caaagtccat ccattaccca
     1801 ctgaacagga cttgaaaaaa attcaacaaa taataaaaaa aatacttgct tcttccaatc
     1861 agcaggaaag gcataacttg aatcttagag aaacgtccat caataaagat actttagaag
     1921 actttagcaa ggaattgatt ttgaaagctt atacgattta tgaacgttta cttgtcattt
     1981 tggaagcaga attgatccaa gaatatcgag aagctttttt gcttcatgta ttcttaatgg
     2041 ttcatcgtat cacatttgat caacaatata tggcagaagg tcgtcaagta gaccagatta
     2101 ttttagacaa aggaacactg gtaggaaaaa tcgaagcagt ttttgcagaa aataatttag
     2161 gaatcaatca gtcagaaatc agtgcattat tctcatatat aaaagaagga gcgtcaagtt
     2221 agtgaaaatg tcagacgagg ctttaaaaat tatcgaggaa agtcaaaatc gggaaaagtt
     2281 aaaacaagtg attgatctaa ttgaagagcg attagtagaa catgctattg taccaaccga
     2341 gttgcagtgg accattctaa ttaatcactt gaatgagatg ctgaaacgtt ctcaaagaaa
     2401 ggaaacgatt cccgtagtcg atcgggattt gttcaaagaa gtatccaaag aagccataac
     2461 tatttctgat gaaatagtta gaagtatcgg taatttaacc gatgatgaaa tatatgtttt
     2521 gtcgattcat tttgaaacag caaaaaataa t
//
LOCUS       DACDYI010000144         2455 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.150, whole genome shotgun sequence.
ACCESSION   DACDYI010000144 DACDYI010000000
VERSION     DACDYI010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.150"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            132..344
                     /locus_tag="IWM52_002864"
     CDS             132..344
                     /locus_tag="IWM52_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9975039.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..1024
                     /locus_tag="IWM52_002865"
     CDS             506..1024
                     /locus_tag="IWM52_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HAP9975040.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
     gene            1031..1543
                     /locus_tag="IWM52_002866"
     CDS             1031..1543
                     /locus_tag="IWM52_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9975041.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
     gene            1814..2437
                     /locus_tag="IWM52_002867"
     CDS             1814..2437
                     /locus_tag="IWM52_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9975042.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          944 a          310 c          409 g          792 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agacatatta aatatgtata gagatattac
      721 ttttagtaaa aataaattaa aagatataaa ttcaattgat aattattata tacaatttaa
      781 aggttttgac tttaatagct ctaccgaatt taagttagca ctttatgcac aattttttat
      841 tgaaaaatta aataggtttc aagaaatagt tgaagatagt gattttaaca cttttaatag
      901 ccataaaccg atgagaggta cttatataaa atttctcgaa aattataatg atttaaaatc
      961 taccaataat tatcaatttg gaaacttgtc ttatgagatg ataagtaaag ttctaagttt
     1021 gtgatgataa atgaaattaa atagtataaa tgatttattt gctattatag gtgttattgc
     1081 taacatactt ttttcgtgtt tgaattttag atttactcaa aagaacaaaa agaacaaact
     1141 cagtatagaa caaatgaaaa aagacattga aactgattta aaaaatgacc ataaagaaag
     1201 aagaaaaatt gaagaatata cttctttaat ggaaaagtgt aaagcaactg atgcaatttt
     1261 tagcttttta agaaaaagtg atgagataat cacacgtgaa aatatagatt tagaaaatga
     1321 gattagtaaa cttgaatcta tcttttccga tataaattct cttaagtatt taatcgtcga
     1381 agaaaattcg gatagctttt tagaatttga aaaatatcta acagatctta tagataatag
     1441 aaataatgaa ttaatgagac aaaacattgc aagattcctt actaaattag tttctgaatg
     1501 gaaaagtatt atttctcaaa atatggataa attaagatat taaggatgtt agttttaaaa
     1561 taaactaatg tccttatttt tgtgttcaca tttctgttac ctaagaaata cttaaaggag
     1621 gtgatgtagc cagcaacgac tacaacttga acccgctatc tacagtttat ttgttgtttc
     1681 ttttgggtat actctaatgg tacaaatcca tatttgctta aagcgttgat ttttcagcgg
     1741 tttttcttta ttgcaaagtg taccctaaga cctgtatact aatagtaaat agattggata
     1801 ggaatgatgg caaatgtatt tagcttattt aagagtttct tccgacgatc aaagtttagc
     1861 tcggcaacat aagctaattg aaaattggtc agtcaaaaat aatattgctg aagacgattt
     1921 aaaaattttc gaggaaaaag tctctggaaa aaatattgaa aacagaccag agctgcaaga
     1981 gcttatttct ttcatacgtg aacgagatac gattattatt cctagtcttg atcgattagc
     2041 aagaaatagt aaagatacaa aagacttact tcaattgttt cgtagtaaag gtgcttctat
     2101 tgaaatatta gacctaccgt cgtttaatgg ggtaactgac cctgcattaa gagacttgct
     2161 aacaaattta gtcatagaag tgtttagcta tgttgctgaa aacgaacgga aaaaaatcaa
     2221 agagcgtcaa aaacaaggca ttgaaattgc aaagaaaaat ggtcgttata ctggtaaaaa
     2281 gattcaatac catgccaatg caacaggaaa aaacaaattg atttacgatg aaattgttga
     2341 tggtctgaag aaaaaagaaa ctatttctaa aatagcaaag aacgcaggag ttacaagaaa
     2401 aaccgtttat cgagtaaaaa gagatttagg aatgtaatat gttccagtta gaaag
//
LOCUS       DACDYI010000145         2389 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.151, whole genome shotgun sequence.
ACCESSION   DACDYI010000145 DACDYI010000000
VERSION     DACDYI010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.151"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            872..1840
                     /locus_tag="IWM52_002868"
     CDS             872..1840
                     /locus_tag="IWM52_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9975043.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            1857..2381
                     /locus_tag="IWM52_002869"
     CDS             1857..2381
                     /locus_tag="IWM52_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP9975044.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
BASE COUNT          920 a          407 c          406 g          656 t
ORIGIN      
        1 ttatgagcga actaatgcaa aatttggcta gcaaaaaaag ttaaaaaata tatgctaata
       61 atcgagcgaa atgaacactt tttcaagtga gcgaatccgc catgacaaag ctgaagcagt
      121 ttatttcagc tttgtcatgg cgtgtgccta gacttttaaa aaagtcggca cacgagcaaa
      181 tatctttatc aagctctaat ttgcgtttta agacgtttga aacgtctaag tatataaata
      241 tataagagta ggtttaagag tgcttaaatc gaattttaga gggcattcag acaactttag
      301 ggttgttagg gcttgccctg accgcctgta aggcgcttta agaacaagat aagggattta
      361 tactaagact gtcttaaaac gtctcaaacg aaggctcata gcttctcaaa gagttcctta
      421 tgaacatttt tagcaaagga gccggaagcg actgcgcagt ataatgtgag agcagagcga
      481 tcacaccttt ttaagcgact gtagggagta agattcccag ttgagaaaaa tgcgcgaatc
      541 tgtttggaga aaaaagaagt ggttttttag gcgggaaatt ttgtccccct cttttataat
      601 ctctttttaa acctcttttt aaacctcttt accgtcggct atatccttac tctcccaagg
      661 gaatgacgct tttatctaca tcgttttgtc tgtttatcta catcgttttg tctgtttatc
      721 tacatcgttt tgtctgttta tctacatcgt tttgtctgtt tgtacattga attagtacat
      781 cgacgatgtt aatataagat gtagataaac aggcattttg tatttcataa cgtgtatctc
      841 ttgtgcgagt aagaaaggag ctattttaaa tatgtctaat gaaatcgtaa agtatcatca
      901 tgaattaaat acaattccgt tgcgaaaatt tacaccagtc gaaatgaatt tgttcttttc
      961 tatcgtatca agaatgcgtg atgtaggtga taaaaaagta caatttacat tcgagcaatt
     1021 aaaagattta agcaattata aagcaactgc aaatgttcga ttcatagatg atttagaaac
     1081 aacgtatgac aaattaatgg atttacgttt tggtagaaga agtgctgacg gtttgcaacg
     1141 tgaacgtttt gttttattta atcaatttaa aatcgacgga aaatctgata ttccttttgc
     1201 tgaaatacaa gttcatgaaa aagccttacc cttacttaat aatcttgaag aatgggtgcg
     1261 ctattctctc caacaattca atgaattaga aagcagttat tcaaaaacaa tgtttcgatt
     1321 actaaaacaa tacagaacca aaggtttcgc ttatttttca aaagaagact ttcatgaatt
     1381 acttgatatt cctaaaagtt acaaccaagg tacgattgat caacgtgtgt tcaatccaat
     1441 acgacaagaa ctaaccgcaa tctttaaagg tttaacaatc aaaaagaaat acggaaaagg
     1501 acggggaaag cctgtcattg gctatcaatt tacatttaaa cctgagataa aaaaatcaga
     1561 cgactttaac aaacatgccc caacaaaaga agaacaaaac aaagcattag aactagctaa
     1621 ccaacaagtt caatctgaaa aagcacctac ttcattacaa gaaaaaatca aagaacaaaa
     1681 gcgaaaattg gaacttttac aacagttaga aaagttagaa agagaacaaa atacccaaaa
     1741 taaagaaaat aatgcacaaa aatcagagat aaattccgat attccttcag aactaaaaaa
     1801 agacttatta gacaatcttc aaaacttatt caaagattga ggggttttta gattttatga
     1861 cagaaaaaac aatcaaagaa cttgctgaag aattaggagt tagtaaaaca gcaattaaca
     1921 aaaaagtttc tgacgaaaat cgaaaacttt ggttttcgaa aatcggaaac aagtttgtga
     1981 ttaatgaaac cggacaaaag gctataaaat caatgttttt taacgaaaac gaaaactcaa
     2041 atcaacaact agttggcgaa aaaacgaaaa ccgaaaacca caaaaacgcc aaccaagttc
     2101 ccgaattaga agtcctttct ctcctagaac aacagttaaa tcaaaaagat atgcaactac
     2161 aagaaaaaga cgagcaaata aaacaaatgc aaaaattact cgaccaccaa caacaactca
     2221 ccctgcaagc caaccaacaa atcgagaaac tgcaggaaca actacagttg acttatacgg
     2281 aagaatcttc ggaaaataaa tcaacgactc tttctgaaga aacagaatcg atagaaaaac
     2341 aatcaaaggc ggagaaaaaa tggtggcaat tctggcaata aaaccagca
//
LOCUS       DACDYI010000146         2366 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.152, whole genome shotgun sequence.
ACCESSION   DACDYI010000146 DACDYI010000000
VERSION     DACDYI010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.152"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..605
                     /locus_tag="IWM52_002870"
     CDS             <1..605
                     /locus_tag="IWM52_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9975045.1"
                     /translation="PKGTTYTWKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVD
                     KKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEA
                     GTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQS
                     SKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            739..2082
                     /locus_tag="IWM52_002871"
     CDS             739..2082
                     /locus_tag="IWM52_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9975046.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            2194..>2366
                     /locus_tag="IWM52_002872"
     CDS             2194..>2366
                     /locus_tag="IWM52_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9975047.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSEL"
BASE COUNT          938 a          301 c          422 g          705 t
ORIGIN      
        1 taccaaaagg aactacttat acttggaaag aaaaagtaga tgttagtaca gctggaaaca
       61 aaaaaggtac tgtagtagta acatatccag atggatctaa agaagaagta gaagttacta
      121 tttctgtagt agataaaaaa gcaccgaata aacctcaagt tgatcctatt acagagggtg
      181 accaaattgt tactggtaaa actgaaccaa atgcagaggt gacagttaca ttaccagacg
      241 gaagtcaaca ccatgggaca gctgataaaa atggtaattt tacagttaaa gttcctaaat
      301 tagaggcagg gacaaaagtg atagtaactg caactgatga atctggtaat actagtgaac
      361 ctactaatgt agttgtttct tccaacaaaa aagacagtgg aaaaaacggt agtaaaggta
      421 gtaagactga caatcaaggt agcaacagca atcaagacaa aaatagaggc aaaagccagt
      481 cctcaaaagt ttttcctaag acgggagaga gtgacagtaa tatctttact attagcggtg
      541 gtttaatact gttaggaacg ttaggattat tagggtataa aaatcgaaaa aaagagaatg
      601 aatagaaaat caatttctta agtcttctct gagaagagga agaatctttt tcttcctctt
      661 tttgcttttt tatgatggga aataagtgtt agttaaagat gaatatactt tttatagaaa
      721 ggaaaagaat gtaattaagt ggaaaaaata gtaattattg gttcaaacca ttcagggcta
      781 gccgcagctc gatttctaaa cttaagcgat tcaaattatg aagttattct aatcgataaa
      841 aaatcagaaa ttagttactt ggaatgtggg acatcgttat ttataaaagg agaagtaagt
      901 aatttagaag atctttttta tcaaaataga atgacactaa attctgagat aaaaaagtta
      961 tttctagaga cagaagttga caaaattgat attcaaaata aaaagattag ttgtgtaaaa
     1021 aaagatggta caagattctc tcaagaatat gacaaagtta tattagctac aggttcaaag
     1081 caaattatgc tagatatacc ggggaataat ttagctaata ttttttcgat aaagaatttt
     1141 ccaaatgcaa aagagatttt taaagctttg gatgatagta ctattaaaaa agtaggaatt
     1201 attggagcag gatatattgg agttgaaatt gctgaagcca taagaaaaag agggaaagaa
     1261 gtatatcttt tcgatgttgc agatagagtg ttgtcgactt attacgaccg tagtttttca
     1321 gataaagttg aagaaatact atcaaaaaat ggaatacatc tttgtctatc agaaagtcct
     1381 aaaaaatatt taggaagaaa aaagattgaa aaaatagtca ctacaagtgg tacccaatac
     1441 tctatggata tggtcgtaca agctatagga tttctatcta atagtccaat tggggaaaag
     1501 gaactcttga gagatgttag tggagcatat ctaactaatg aataccagca aacaaatata
     1561 aaagatatat atgctattgg ggattgtgct acaacttttt ttacgtccat taatagaagt
     1621 agtgttgatt tttctatttc taatgctcta agaacagctt atattgcaac tcaacatatt
     1681 aacaatcaag attttacacc ttctggttcc caatttacaa atggcttttc tatatttaat
     1741 tacaattttt acgcagcagg tttgaattta aaaacagcac gattattaag tgagtctata
     1801 gattatatag aaatagaaga tttcattaga cctgcttttt tgagaaaaaa tagtcgtgta
     1861 aaattaagaa ttagctttga aaaaaataca aagagaatag taggtgtaca actttgtagt
     1921 caagaagatt tatctggact attatcaatg ttttctcttg ctatagaaga acaaattaca
     1981 ttagacaaat tgaaaatgtt agattattta tttcatccta gttttagcca accctataat
     2041 tttattttaa aggcagtaac aggattaaac attgaaacat aatcgttact aaatgaaatc
     2101 aagaaatata ctgtattact caattcattg aaaatattta tcataatttt taatagaaaa
     2161 cactattaaa gagaaaaaag agaggtaaat caaatgaaaa aatctttaaa acttgcatca
     2221 gcagttatat taatgtccca actccttata gcaacacctt tccatgtgtt ggcaagcgaa
     2281 aattcaacat acaaaattgc aaaagaaaga accgagacga ctgaaatgag taattccgat
     2341 gtaacagcag agagtagtga actttt
//
LOCUS       DACDYI010000147         2340 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.153, whole genome shotgun sequence.
ACCESSION   DACDYI010000147 DACDYI010000000
VERSION     DACDYI010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.153"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(365..1657)
                     /locus_tag="IWM52_002873"
     CDS             complement(365..1657)
                     /locus_tag="IWM52_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9975048.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
     gene            complement(1931..2191)
                     /locus_tag="IWM52_002874"
     CDS             complement(1931..2191)
                     /locus_tag="IWM52_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9975049.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          781 a          441 c          355 g          763 t
ORIGIN      
        1 tatgggctac tctgtgatac ttattagcca ttaaactatt ttacccacat agcatatatt
       61 ttgaaatcgt tcattccgta aattcaacta aatatgccta aaaaaataaa aaatctaaga
      121 tagaataaga tttattcagt cttagatgga tttatagcaa ctttttcgta acaactttaa
      181 aaaatacgaa aagaaagagc tgttttactc tgactacgtt gctaaattgt cgtttaatca
      241 ttcatttaaa ccaaatcttt ttttatagaa ttcgttttaa tcctcctgaa tccaaaaact
      301 atgaaaaccg taattaaata gttaagagtt tttaaccacc ataattctct cggctatttt
      361 gaaatcagat aaattttgat cggacacata gttgaataaa cctaccgttg ttacggaagg
      421 taaatcgcat acttttcatt ctagaggaga aggtctcgtt gatcattaac tgattaagca
      481 ccatagccca gttttgaatt cgaccacctg accacttcgc gtgtaattct ttcgtgcgta
      541 aataaagtag ttttaggagt gcattctcat tagagaacgc tccttttttt gtgacttttc
      601 tgaagctgga gtggacactt tcaaccgcat tggtagtgta cataattttt cgaatggcac
      661 taccataatc aaatagttgt tcaacatgtg caaagttccg tttccagaca tctacagcac
      721 cagaataatg agaccaccga ttttgaaaac tgtcaaaagc agcatgtgca gcgtttagag
      781 aagaagcacc gtagaacttt ttcatatctc ggcagacttc cttatagtcc ttacttggaa
      841 tatagcgcaa tgcatttcga acaagatgaa caatacagcg ctgaacaatt accgatggaa
      901 agatcgcttt tgcgccttct tcgaggccag aaacaccgtc catcgaaatg aaaaagacat
      961 cttcgacacc acgtgctttc agttcatcaa atacttgcat ccagcgattt ttagattctg
     1021 tttgatttaa ccataatcct aaaatctcct tatttccttt gagatcatag ccaagaatgg
     1081 tgtatacagc atattctttg gcttcataat tttctcgtaa agtaacatac atacaatcaa
     1141 cgaatagaaa ggcataacac tttgctaggg ggcgggcttg ccattcttcc aattcaggaa
     1201 ggacagcgtc agtgatatct gaaatcattt catgggaaat atcaaagcca taaatagctt
     1261 cgacggttgc ggcaatatct cgttgactca ttcctcgtgc atacatggaa agaaccttcc
     1321 cttcgatgtc ggagacatct cgttttctct taggaattaa ctctggttca aaggaagctt
     1381 cccggtctct aggaacatca atagctactt caccaaaact ggttttaagc gttttagttc
     1441 catagccatt tcgacggtta tcgtgttcct taggctcttt agaatgggca tcataaccta
     1501 aatgattatt caattctcct tgaagcattt tttcaaaaag gggcccaaac acatctttca
     1561 aagcatcttg catgtcatcg acagattcag gttgataggc attcagaatg gattcagcta
     1621 acttttcggc atcaggattt cttttctttc tagccatcgt gtaatcacct cttgatctta
     1681 ttgtagaaaa agaaaaaccg cgaagcaacc acctgcctag gattaatggt tacttacacg
     1741 gtttatatta cactctcatc attaaaaaag tatctttcga tattactaaa caatgaaatt
     1801 atctatttga aatttaaatc tgataatata ttgagtgttc caatgagggc tttgatccta
     1861 ttatccatat ctttttcagt tttaatattg atacacatta taagaaaaag aaacttttgg
     1921 ttaatttatt tcatcccagt gcaaaatata aaaaagtaaa atagaccata ggcaatagat
     1981 taacaattaa tataatacaa gcaaatattc tattcattgt atttctgctt tcaaaaagtt
     2041 ctaatattga aagtataatt ccacaaatgc ctataataag tatgggaata agattagaaa
     2101 ataacacccc gagccaatct gtgaaaatta ctatatttac taatatccct attaacatac
     2161 ctatcatatt tattaaatca atttttttca ttagatattt ctcctttatt aaatctgaag
     2221 tacttatatt aaaataattc taaactattt acctcaaaaa gtgcaataaa ttatttctga
     2281 acaataaaat gatagtcaaa acaaaaaaat attgttaatc gttcagagcc atccgagagt
//
LOCUS       DACDYI010000148         2260 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.154, whole genome shotgun sequence.
ACCESSION   DACDYI010000148 DACDYI010000000
VERSION     DACDYI010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.154"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            555..>2260
                     /locus_tag="IWM52_002875"
     CDS             555..>2260
                     /locus_tag="IWM52_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP9975050.1"
                     /translation="MNENGEFEESKKYQATSEEEKAAVRELLEEAVPTDFLKEKENDH
                     FYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYYKFYFEEVKNGEVV
                     DQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKEQMQSKETKTKQKG
                     YQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFPERSFRNQLLIEEQ
                     FPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGFVREIEATPEDKKR
                     IESGQIEVIKKTYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQLDKKGQSELQAGIQ
                     AVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNTRNTPTQHLATSIH
                     ELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTLEKAIPYIAAWTKNGQTLDDK
                     EAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQEHKNTVETAEQKNI
                     VPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGKSYGKEIQTALPSE
                     EFA"
BASE COUNT          907 a          361 c          500 g          492 t
ORIGIN      
        1 aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa ttagtacgat
       61 tgcaaaatga attaaacgaa caaattgaaa agatgtttga acaccagaga aagaccaacg
      121 gtcaaccaat gaacgataag agaaatgggc attcttggtt taggcaacaa gagcgaatcg
      181 aaaataaagt gcatagtctg agagaagaga tcaagcaaca agaaaaacaa gtagaaaaac
      241 ttgaaagaca agaagagata aaggaaatgg gctataacaa gtacggcgga ctggatatga
      301 cgatcgagaa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa ggtgaatcga
      361 ctttttcagc cgctacgatc cgaaaatacc aacggaaatt ggaaacatta gaacagctga
      421 aagaaagatc ggaaaaagga aaagaaaacc ttcttcctga agtacaagca atcattgaca
      481 gcggacgagt gacgcaatgg aaaaaaaatc cgaccattta ttttctgaaa ggctatcgaa
      541 aagtcgcttt agaactgaat gaaaacgggg aatttgagga atctaaaaaa taccaagcca
      601 cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctgta ccaactgatt
      661 ttttgaagga gaaagaaaac gatcattttt atcgagtaga attcaacgaa aatgatccag
      721 aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt cgaattgatc gatcaactca
      781 aaacgttgga taaccaatac gccgcagaag caggttatta taaattctat tttgaagaag
      841 tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc gtcaacagta
      901 atcaacccat ctatttagag ttagcaaaat tgtgcgaaca agtacctgag aaggaacaaa
      961 aacaatcggt caaaaatgaa cctgtttcga aagaacaaat gcagtcgaaa gaaacaaaaa
     1021 caaaacaaaa gggctatcaa aaacgaacgg cagaagagat taaacaagaa gttaaatccc
     1081 tttcgcaaca ggcactggat gcggtgaaaa agcatactca ttctcctgaa gacgtcaaag
     1141 aacttttgaa ctttatgtct cgttttccag aacgatcctt tagaaatcaa ttattgattg
     1201 aggaacaatt tccaggtgca acggcttgtt taggtcgtgc cgcgttgaaa aaagaaggaa
     1261 tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg gtcaaaggat
     1321 tttatgagaa aggacgtggc tttgttcgag aaatagaagc tacaccagaa gataaaaaac
     1381 gaatcgaaag tggtcaaatc gaagtgatca aaaaaactta ctacacgatt gaaaaagtgt
     1441 ttgatatcac acaaacacag ttaaaacctg aagactatcc gaaaatcttt ccgaaccgtg
     1501 tctttgattt tcaattggat aaaaaaggac agtcagaact tcaagctggg attcaagcgg
     1561 tagccaacaa tattggaata caaattcgag atatgacaga aagtgaagtc tatcaaagag
     1621 aattaggaca agcacgtggg gcgtatgttc gtaacgaatt tactggcgaa gaagaaatcg
     1681 tcatgaatac tcgaaatact cctacgcagc atttagctac aagtattcat gaattagccc
     1741 atgcaagaat gcacaaattt tcagaattag ataccgcaac taaagaattg caggcagaaa
     1801 tgacttctta tattgtgtgc aaacattttg gaatggacac gttggagaaa gctattcctt
     1861 atattgcagc atggacgaag aatggtcaga cgttggatga taaggaagct gcggaacgtg
     1921 ggaaaatcat gaatgatgtc agccgtgtcg caaatgaatt cattcaaacc atttctaatg
     1981 aaatcaatca atacagagaa agagaaccag agatccaacc agcagaagtg aagcaggagc
     2041 ataaaaatac tgtagaaacg gcagaacaaa agaatattgt tcctgttggt tcactttgga
     2101 tcgacaagaa agacgggaaa gtaatggaag aagatacggg tcgaacgtta catttgaagg
     2161 atgcggcgtt tattgaagat ccacagcata atcaagtaat tttaaaagga aaaagttatg
     2221 gaaaagagat ccaaacggcg ttaccttcag aagaatttgc
//
LOCUS       DACDYI010000149         2180 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.155, whole genome shotgun sequence.
ACCESSION   DACDYI010000149 DACDYI010000000
VERSION     DACDYI010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.155"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            700..>2180
                     /gene="ltrA"
                     /locus_tag="IWM52_002876"
     CDS             700..>2180
                     /gene="ltrA"
                     /locus_tag="IWM52_002876"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP9975051.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKP"
BASE COUNT          858 a          336 c          455 g          531 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc
//
LOCUS       DACDYI010000150         2180 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.156, whole genome shotgun sequence.
ACCESSION   DACDYI010000150 DACDYI010000000
VERSION     DACDYI010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.156"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            948..1421
                     /locus_tag="IWM52_002877"
     CDS             948..1421
                     /locus_tag="IWM52_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAP9975052.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1430..1657
                     /locus_tag="IWM52_002878"
     CDS             1430..1657
                     /locus_tag="IWM52_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9975053.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          707 a          345 c          321 g          807 t
ORIGIN      
        1 tttcggagaa ttaaaaaata caataaataa ctttagttac tttaatgcgt tcttacttta
       61 tttttatatt atcatttttc ttattcactt ctttggttta tactattctt ataaataaaa
      121 acgaggtatg tcctatgatg aaaagattgt actattcatt gatcataacg attggctatt
      181 tgattgtatc aaatctagga aatatggtat ttggtatcag taaagaattc agttggacta
      241 ctacattatg ggaatctcta ttctttttca tcttcgtatt tttgttacaa aattatcgaa
      301 aaaaataaac atgtgccatc tatctgttgg cacatgtttt aggatttatg gtacttttta
      361 ttcggcaatc cattttattt ttctacttct tcttaatacc tctaacactt ttgatttata
      421 aactaatcca atgcatctac ttgcgcttat taagctattt cagctagata gtaccacccc
      481 atgcgtcaat gcttgatttt ccttttgtat aacattgtat aagcaataaa ggacaaataa
      541 gtggtgatta caccaaacca ttgaataaga aaaattctga tacgtaaata aacatgaact
      601 tgaaaaaatt aatgaatcgg ttagctacta cgtagttgtc ttttttctca tacagttctg
      661 ttaacttaca cttctcttcg ctagataata aaaaatcctc caactgtatt gtccaatata
      721 atcccaggca tctaaactag cattatagaa gagacacaca tagaatctaa aataactggt
      781 tttaaatgtt tcttacaatc aaaaaaagct gttaaatgca ctccacacct tcatactcta
      841 tcatttttta taaacgatat atttttattg tttttcgata aaaaatagtt gttttacaat
      901 aattattctg ctatatttta gctatcaaag ataaaagagg gataaaaatg aaatttaaaa
      961 cattgctatt aaaatttgtc gttttaggta taagtgccgt atttattttg ttcggcgtac
     1021 ttttttatac tcagatcgct acaagcgaaa aaggattcaa ttttgattgg cagaccacat
     1081 tatttgtttc tgtcatttac tttgcccttc tcctagggat cgttattgct tatttcttat
     1141 tgaaactatt gaaaaaaatc gaagataacc aagctttttc cactcaaagc ttaaacatac
     1201 taaaaaaaat cgaacgaacc attttgttta ttggtttcgc cttttttggc ttacttccta
     1261 aatttatgaa agtagcggat ttagacgatg ctccaggttt gatgttggta ggaatcgctg
     1321 tcgtattcat tccttttgca gtatacacat taacaagcgt gctggaaaaa ctgcttgaac
     1381 atgcgatttt acttaagaca gatcatgatt tgacagtgtg aggtgcgaca tggcaattat
     1441 cgttaattta gatgtagtat tagctcaaaa aaaaatgagt gtgactgaat tgagtgagaa
     1501 agtgggcatt acgatggcaa acatctctat tctaaaaaat ggaaaagcaa aagccatccg
     1561 tttttccact ctcgaaaaaa tctgccaagt tttggagtgt caaccaggtg atttgttgac
     1621 ctataaaaat gactcgaata ctataccaga agaataaaat aaagtagaac aaacagtaaa
     1681 taaaaaataa tctgaatgat agatgtgtga gacttttaga agttatttac gtctattatt
     1741 cagatttttt atttcgattg aataccgttt tatctataaa taagatatgt actgactcat
     1801 acaaaggggg ccttttcttc tccatgtact atcacttttc ctgatacaac tggtttcctg
     1861 ttacgttgta gttggatctg cttttagtat aatactttat tcattccacc agactgatag
     1921 cagtttgagc agtctaaata atcatgtagt attacaggcc attctgtttc ataccttgaa
     1981 gtgcttaaaa cttcagctaa tggcccattg tgttaactct tactccattc ttttttagta
     2041 agctcccata tatactgtaa agccttctat atattatgtc atgttgcttt ttaacattta
     2101 ctatgtagtt ttgataaaat gatttgaata acagtgatgg acaattcttt tttattttta
     2161 acattatttt gataataatg
//
LOCUS       DACDYI010000151         2145 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.157, whole genome shotgun sequence.
ACCESSION   DACDYI010000151 DACDYI010000000
VERSION     DACDYI010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.157"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(118..708)
                     /locus_tag="IWM52_002879"
     CDS             complement(118..708)
                     /locus_tag="IWM52_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAP9975054.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKKIRLYKLGFYDYYKKYGDPFS"
     gene            1231..1392
                     /locus_tag="IWM52_002880"
     CDS             1231..1392
                     /locus_tag="IWM52_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9975055.1"
                     /translation="MEPKDKYLDYARVNKDKFIETIIKGKIPDDEKDAVFMAGSPGAG
                     KTEVALGLA"
     gene            1411..1905
                     /locus_tag="IWM52_002881"
     CDS             1411..1905
                     /locus_tag="IWM52_002881"
                     /inference="COORDINATES: protein motif:HMM:NF018154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin"
                     /protein_id="HAP9975056.1"
                     /translation="MIDADYFRAQFPEYNGKNSSVFQKASSWLVEQSLKYVLEHGYSF
                     ILEDTFAILSAEKNIIRALKNNFRVTIFYVYQDPKVAWDFTRKRELAEGRHVPKEMFI
                     NAFFKSRENIEKVKNRHPEVVLHIMIKDYQNDISEVHYAADNIQLVLPIQYTSKELEE
                     ELHD"
BASE COUNT          774 a          332 c          364 g          675 t
ORIGIN      
        1 tatgaatata aatagagtgt atcttctttg ataaatgtca gaaaaagtgt gacaaataac
       61 catcagtaaa attacttgtc atactattga taaggagtat ttaattgaag agaatgatta
      121 ggagaaagga tctccgtact ttttatagta atcatagaat ccaagtttgt atagacgaat
      181 ctttttttct cttatttctt ctgtttcaaa atggactgct tgaagaatca agtctgtgaa
      241 ttctatagga gctgttccat tcgcagtaat caaattacga tctttcacag cttgttgttc
      301 tataaaatct tctttattct gataattttc cgattctaac caaggaaatt gcgcatttcc
      361 ggtgtgtttg taaccttgca ataatccgct ctgagctaaa taatcaactg ccccacaaat
      421 agcagctact ggtacgtctg tttttaacaa tttatcgatt atttgatata aatgatgatt
      481 ttttatgtcc catgaattgc caccgatcat aataaaaagg tcaacttttt caggtatttg
      541 ttcgattaag taatcgattt ttgtttttaa tcctccaatg gaagtgacct ctgattggat
      601 agaagcagtt ttgttttgcc atccctcttt ttggtttagt gtgctagtaa tataagcacc
      661 ttcccaatcg gcatattcct ctaatagaaa cgtaatcgca gttttcattt tcaagctctc
      721 ctttactatc tatgacaagg acaacagttt taacatacag acattcatta aatttggata
      781 gcgacagcga aaacaacaat tcttaagttt tagacagagt atacagacag tcaacttaat
      841 taaatacgaa ctaaataaaa atgacaggta atcatttttg tttaccagca ttataacatt
      901 ttgcatacat ggatgacaaa agaaccaatt cccacttgct tgagtacaat cttaaaagaa
      961 actggaaggc aaaaagtaat tatagaaacg aaaaataaat catatcgggc actcagacat
     1021 gaaaacaacc ctagaaatat ataactagtc caatgaaaaa aaagcgaatg ccgtagaaaa
     1081 caaggattct gcgtacattc atatctcatt ttgcgagcat atctgctatt actgtgacta
     1141 aatgttaata tggttaaaat gaccgttata ttttttatat aaatatcgta taattagtat
     1201 gaatatagaa gaaggaaaga aggtgcaatc ttggaaccta aagacaagta tttagattac
     1261 gctagggtca ataaagataa atttattgaa acaatcatca agggaaaaat ccctgatgat
     1321 gaaaaagatg cggtattcat ggctggaagc ccaggagcag gtaaaacaga ggttgcacta
     1381 ggtttagctt aaaattatga caaccatgtt gtgattgatg cggactattt tagagcacaa
     1441 tttccagaat acaatggaaa gaactcaagt gtttttcaga aagcttcttc ttggttagtt
     1501 gaacaatcat taaaatatgt actggaacat ggctattctt tcatactaga ggatactttt
     1561 gctatcctta gtgcagagaa aaatattatt cgagccctta aaaataactt tcgcgttacg
     1621 attttctatg tttaccaaga tcccaaagtt gcctgggatt tcactcgaaa acgtgaacta
     1681 gctgaaggac gtcatgtgcc aaaagaaatg tttatcaatg cgttttttaa gtccagagag
     1741 aatattgaaa aagtcaaaaa tagacatcct gaagtagtcc ttcacataat gattaaggat
     1801 tatcaaaacg acatttctga agtgcattat gcagcagata atattcaatt agtactacca
     1861 atacaatata cttcaaaaga gttggaggag gaattacatg actgagaaag aacaagtaca
     1921 acaaatcgtg aagaagtata ataaaagtat cgctgattta tcagaaaacg cctcagcgaa
     1981 agaatttaaa acagtaatga attacgtagc agatgaagca aatcgtaagc agcgaaaact
     2041 agttgggttg gataaataaa ttttttaaac catatccttg taaagttgag ttttttattt
     2101 taatgaatta agactattaa atagataatt attgtaactt aaaaa
//
LOCUS       DACDYI010000152         2139 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.158, whole genome shotgun sequence.
ACCESSION   DACDYI010000152 DACDYI010000000
VERSION     DACDYI010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.158"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..105
                     /locus_tag="IWM52_002882"
     CDS             <1..105
                     /locus_tag="IWM52_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family peptidase"
                     /protein_id="HAP9975057.1"
                     /translation="LGFTRMDWRQAQGHAFTDDGTWIDPLPFLNSSKK"
     gene            121..765
                     /locus_tag="IWM52_002883"
     CDS             121..765
                     /locus_tag="IWM52_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9975058.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTFKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIEQLKQQDGTKAKEEVTEAQSKTAEEFG
                     KLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            776..1582
                     /locus_tag="IWM52_002884"
     CDS             776..1582
                     /locus_tag="IWM52_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9975059.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTHQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFAVKLSHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQMKDQEEIIQKLQEKIDRMTKEKI"
     gene            1604..1834
                     /locus_tag="IWM52_002885"
     CDS             1604..1834
                     /locus_tag="IWM52_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9975060.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
BASE COUNT          882 a          329 c          400 g          528 t
ORIGIN      
        1 ttaggtttca ctcgaatgga ttggcgtcaa gcacaaggac acgcattcac agatgatggt
       61 acatggattg atccattacc atttttgaat agcagcaaaa aatagatcag gagaaaaaat
      121 atggaaaata aaactttaaa aatcgtctta acagctgcaa ttagcgttag tttaatcggc
      181 aatatcgcgt tgttttggca gctgaaagaa gaacaagaga caaatacctt taaaattgaa
      241 gaagtggcta aaaacgcgtc tgagaagctc aaaaaaaatt cctctgaggc acaggcgaag
      301 atcgacaagt taaatcaaac catcaatgag aaaaaccaag aaatcgaaca gctgaagcaa
      361 caagatggca caaaagcaaa agaggaagtc actgaagcac aaagtaagac ggcagaagag
      421 ttcggtaaat tagccattga taaatcatta agcagaaatg aattaacgga aaaactaaaa
      481 gatgtcgcaa cccaagaagt gatcgacaaa ttaagtcctg cagacgatga tcaccaacat
      541 gatgattacg gaagttactc ctttacatta ggtgacgtgc aaacttacgc tcaaacaagc
      601 agtgtaaaag aggaacaaaa ttttgtcgta tttgttgatt atacaatcgg caatcctcaa
      661 ttcaaagatg tcaaaccaca aaaaataaaa ggtggattaa ctgtcactga aaaacaggta
      721 gatggtcaat ggaaagtcac tgactttagt tatttcactc gttaggagga agaaaatgag
      781 taagaaaaaa ggagaaagac aacgtttcaa aaaacgaaaa agttatctta gatggctggg
      841 aatctttatg attctcggat ttttgattct tgctagtccg atttacacgc atcagaaggt
      901 agacatacca gatgaagtaa gcacgaacgc atttttaaac acgaaggaac taacgatggt
      961 ttcaaaagaa aaaggagcga accagcaatt agtttttgaa tttgcaatcg acgatgaaga
     1021 tcaaagctta ttaaaagaac tggctaatct taattatcgc gtagaagtaa agacagctaa
     1081 aggggattat caagcaataa aagccaaagt gattaaggtg tctgatgatt attttgcagt
     1141 gaagctatcg catgtaccag aagaatatat tgctatgcga ttaaccatta ttcctgaaaa
     1201 aattgatcca aaggtggata tgcaagaacc acaagatctg atttattacg ttcacgaaga
     1261 taaggttaaa gatcaagtga aagatgaaga ttatgaacaa catgcgatca attacaaagt
     1321 gaaaggatac aagaaagaac aaaaagcagc tcaaaaacaa atggaatctt tccaagcaac
     1381 cattgatttg aatgaagaat tagtgaagaa gctaaaagat caattgcctt atcaagtcgc
     1441 agatgatcag gaaaatactg aaaacaagat taatagctac cagcaagaaa ttgaaacatc
     1501 gaaaacgcaa atgaaagatc aagaagagat cattcaaaaa cttcaagaaa aaattgatcg
     1561 tatgacaaaa gaaaagattt agaaggacag gtggagtgaa aaaatgaagc gaacaaactt
     1621 ggctgacaaa gatactttcg ctgagcagaa agtattgatt aaagcctatc tcaaagttgt
     1681 aaaagctaaa gaaatatgtc gagaagtgaa tttatctaat acaacaatat ctgagttgaa
     1741 gaatggtcga aagaatttag atactttaaa attcagatca tttgaagctc tatatgtagc
     1801 tgctaaaaaa tattttacag ctgataacaa ataaaaaaca ggagtatttt atgtgaaaaa
     1861 agtatataaa aaatgcttat gaatcagcta tatcacctta accatttgtt aatgagatgg
     1921 gagaaaacat taaccacgat tctaacgcaa acatcaagca cataaaacat ttaacactcc
     1981 tcctttctgg ctgaagattt caaccaaaca ggaagacagc gtatcaatta tagcatacac
     2041 tttttcttaa attcattttt catatgacaa aatatttcct tctaatcgac aaagttagaa
     2101 ggatttttta ttgtctaaat acacaaataa ccttaattt
//
LOCUS       DACDYI010000153         2079 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.160, whole genome shotgun sequence.
ACCESSION   DACDYI010000153 DACDYI010000000
VERSION     DACDYI010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.160"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..396
                     /locus_tag="IWM52_002886"
     CDS             <1..396
                     /locus_tag="IWM52_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9975061.1"
                     /translation="IIPEKIDPKVDMQEPQDLIFYIHEDKVKDHVKDEDYEQHAINYK
                     VKGYKKEQKAAQKQIESFQATIDLNEELVKKLKDQLPYQVAEDQENTENKINSYQQEI
                     ETSKTQMKDQEESIQKLQEKIDRMTKENI"
     gene            419..648
                     /locus_tag="IWM52_002887"
                     /pseudo
     CDS             419..648
                     /locus_tag="IWM52_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            807..1742
                     /locus_tag="IWM52_002888"
     CDS             807..1742
                     /locus_tag="IWM52_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HAP9975062.1"
                     /translation="MSTLSCVMLLYLCIQINEIMPPHWQEKKKEFKEEIEIKSNDWIE
                     YTWLPLKRNSKRKEFWALQLWPIGKLFIYMASLMVTLIGYLVVVYGVAYYLLSEPKQI
                     AASNIFTAVMTLMCVMCFSIFSYLLVQFIRQNLTMSKLFKSLLDLLILGSMLTTLTDR
                     FEQSEKTVEGIKNFLTTIDQFINGYMYLFEVGFILTLFGCVFIKLIKRSIEKEMVIDL
                     IPTLSGYEYYVDFGKYEELIHTDIVHTSIHEVTVKGIRSTLKDGQETKTIDRRKEVYN
                     FYKVEASLRYGIGKLRLDGSNIQQNKPYVLGKVVI"
     gene            1786..2055
                     /locus_tag="IWM52_002889"
     CDS             1786..2055
                     /locus_tag="IWM52_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9975063.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
BASE COUNT          784 a          285 c          406 g          604 t
ORIGIN      
        1 attattcctg aaaaaattga tccaaaggtg gatatgcaag aaccgcaaga tctgattttt
       61 tatattcacg aagataaggt caaagatcac gtgaaagacg aagattatga acaacatgcg
      121 atcaattaca aagtgaaagg atacaagaaa gaacaaaaag cagcacaaaa acaaattgaa
      181 tccttccaag caaccattga tttgaatgaa gaattagtga agaaactaaa agatcaattg
      241 ccttatcaag tcgcagaaga tcaggaaaat actgaaaata agattaatag ttaccagcaa
      301 gaaattgaaa catcgaaaac gcaaatgaaa gatcaagaag agagcattca aaaacttcaa
      361 gaaaaaattg atcgtatgac aaaagaaaat atttagaaga ataggtggag taaaagaaat
      421 gaagcgagcg aacttggtcg gcaaagatac tttcgctgag cagaaagtat tgattaaagc
      481 ctatctcaaa gttgtaaaag ctaaagaaat atgtcaagaa gtgaattatc taacacaaca
      541 atatctgagt tgaagaatgg tagaaagagt ttagatacct taaaattcag atcatttgaa
      601 gctctatata tagctgctaa aaaatatttt acagctgata ataaataaaa aattaaggtg
      661 ggttctatgt gaaaaaaacg tatcaaaagt ttttatggat tagctatatc gtcctattta
      721 tcgttgggtt tctcgtcagt gtgtccgtga tcgtcggaac gctttttctt ttgttctctg
      781 acgaagatct ggtacttaag gggttaatga gtacgttatc gtgtgtcatg ttactttatt
      841 tatgtataca gatcaacgaa attatgcccc ctcattggca agaaaagaag aaagaattta
      901 aggaagaaat cgaaataaaa tccaatgact ggattgaata cacatggctt cctctgaaaa
      961 gaaatagcaa acgaaaggaa ttttgggcat tgcaattatg gccaatagga aaacttttta
     1021 tttatatggc ttcacttatg gttactttaa tcggttattt ggtggtggtc tatggggtgg
     1081 cctattactt gttaagtgag ccaaaacaga tcgcggcgtc aaatattttc acggcagtaa
     1141 tgacattaat gtgtgtcatg tgcttttcta tttttagtta cttactcgtc caatttatca
     1201 ggcaaaattt aacaatgagt aaacttttca aaagtttatt ggacttactt atcttaggca
     1261 gtatgttaac gactctaaca gatcggttcg aacaatctga aaaaacggta gaggggataa
     1321 agaacttctt aacgacgatt gatcaattta taaatggata tatgtatttg tttgaagtag
     1381 gctttattct cactttgttt ggctgtgtat ttataaaact cattaaaaga tcaatcgaaa
     1441 aagaaatggt tattgatcta attccgacgc tgagcggcta tgagtattac gttgattttg
     1501 gaaagtatga ggagttgatc catacggata tcgttcacac gagtatccat gaagtaaccg
     1561 ttaagggcat cagatctact ttaaaagatg gacaggagac gaaaacaatc gaccgaagaa
     1621 aagaggtcta caatttttat aaagttgaag caagtctacg atatggcatt gggaaattga
     1681 ggttagatgg gagtaatatt caacaaaaca aaccatatgt tttaggaaaa gtggtgattt
     1741 agaacttcct atgaaagatg aataaataag gagaatcttt tgaaaatgat tcttctttac
     1801 agaattgtgc ttatagttgg cacggagtta aaaatcttaa agaaggagca gttattcaaa
     1861 gtgaaagaac atcataatca gttactatgt attaaaacgg gtacgatgaa cattcaatac
     1921 cgaacagaat atttagaatt aacgaatcaa actgattttt tgtcgattcg agagcaacat
     1981 aagcaagaaa aattaatggc tacttatagc caattgattg atgagtggga acaattcaag
     2041 gaatctaaaa cataaaattc catttaaaca ccttatttt
//
LOCUS       DACDYI010000154         2069 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.161, whole genome shotgun sequence.
ACCESSION   DACDYI010000154 DACDYI010000000
VERSION     DACDYI010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.161"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            161..583
                     /locus_tag="IWM52_002890"
     CDS             161..583
                     /locus_tag="IWM52_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9975064.1"
                     /translation="MAKVIDKHIYFHQTHIDVLDEIISTHAEVKNYSEAVRFLCMNYD
                     PEVEKKVNSNQAKLNGMSKEISILTEMVSEFADLHLKDTGILIGKNSTVYQEAKERVE
                     GKIKSNQTKKYSPQLKKPVNESVHARVDPFDPTKILKR"
     gene            602..>2069
                     /locus_tag="IWM52_002891"
     CDS             602..>2069
                     /locus_tag="IWM52_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9975065.1"
                     /translation="MTQSPSVILTCQFTLPNKKSFSTYIDYITREQALEEKERRTPEE
                     EQELQRIKNALEEFYIPEGETFSESKTGKEFSETSNEAQSLMEHGINFDKLEEQDFTN
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSIASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWNYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQR"
BASE COUNT          840 a          315 c          400 g          514 t
ORIGIN      
        1 cgtcctttga tggaatacaa acaagaaaag tttgaccagt tattagaaaa gttttcagaa
       61 attcaaagtt ataacacaaa gatattagaa gaatatgtta gccaaaataa tcgtattata
      121 gaaatcttga ttgagcaaaa tagaggaagt gaactttgat atggcaaagg ttatagacaa
      181 acacatttac tttcaccaaa cacatatcga tgtattggat gaaattattt ctactcatgc
      241 agaggttaaa aactatagtg aagctgttcg ctttttatgt atgaactatg atccagaagt
      301 ggagaaaaaa gtaaattcca atcaagcaaa gttgaatgga atgagtaaag aaatttctat
      361 tttaaccgaa atggtttcag aatttgcgga tcttcatttg aaagatacag gaatattaat
      421 tggtaaaaat tcaactgttt atcaagaagc aaaagaacga gtggaaggaa aaattaaatc
      481 caatcaaaca aaaaaatatt cccctcagtt gaagaaacct gtgaatgaat ctgttcatgc
      541 cagagtagat ccatttgatc caacgaagat tctaaaaaga taaaaatgaa ggaggataat
      601 aatgactcaa tcgccttcag ttattctcac ttgtcaattt actttgccga ataaaaagtc
      661 attttctact tatattgact atattacaag ggaacaggct cttgaagaaa aggaacgacg
      721 tacgcccgaa gaagaacagg aacttcaacg aatcaagaat gctttagagg agttttatat
      781 tcctgaagga gaaacttttt cggagagtaa gacgggtaaa gaattcagtg aaacgtcgaa
      841 tgaagctcaa tcgttgatgg aacatggaat caattttgac aagttagagg aacaagattt
      901 taccaattat ctttcttata tgacacgtca ctatgcgtta gaacaaaaga aagaactgac
      961 gcaaagtgaa caaaaagaat accaacgctt gaaccaagca atgaaaaaat atcaaacaga
     1021 accccataaa aaagcttcag gaaatcttcc tggagttttt tctatagctt cagacgaagt
     1081 gaaaggaagt gatctgaagg agattaagca aatcttccaa agaggacaac aaaaaggctc
     1141 aatcatttat caagatgttg tttctcatga taattcgtat cttgaaaaaa taggattgta
     1201 cgatcccaaa acagatactt tagatgaaga agggttaaaa gcagctggaa gaaaaatgat
     1261 ggagacatta ttcgagaaag agcaactgaa cgaaacaggc tactggatgg caacgattca
     1321 ccgtaacact aaacatatcc atattcattt tgcagttgtt gaaaaagaaa atacgcgtaa
     1381 accttacacg gaggttgaaa atggtgtgag ttatatcgta ccgaaaggta agagaagaca
     1441 agcaacatta gacgcaatga agaccaatta tgtacatgag ttagaacgtt tttcttatga
     1501 aaaggatcga atgattggta tggagaaacg aaatttattg acgcggaaaa gtgatttgcg
     1561 taacacctta acaaaagtgg taaaagagcc agcgcgttac gatcgacacg cgatgaactt
     1621 attaaatgaa gtatataaaa ccctacctga caaacgttcg gaatggaatt acggggatga
     1681 aaaacgaacc aaactttcac catacacaag agagaaactc gatgagttaa ccaaatatgt
     1741 tttaacaaaa gaacctgaat atcaagaata tatacagatt tcaaaagcat tgaaagaaga
     1801 agcaaaaagt ctttatgggg aaagcaagag agaatcaaag gatgcagaga aaaatgctct
     1861 ctttgattta aaaaagcgca caggaaatgc catcctaaca gagctgaaga gacaggatag
     1921 tcaaataaaa ccattaatag aaagtgttga tcagatgcaa tctatttaca atgaacgatc
     1981 ctttcgaacg cttaaacaat tgccaacgaa tgaatacaaa tatagttacg aagaattcat
     2041 gaagcgcagg aaacaattcc aacaaagag
//
LOCUS       DACDYI010000155         2014 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.162, whole genome shotgun sequence.
ACCESSION   DACDYI010000155 DACDYI010000000
VERSION     DACDYI010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.162"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            100..570
                     /locus_tag="IWM52_002892"
     CDS             100..570
                     /locus_tag="IWM52_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAP9975066.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            792..1895
                     /locus_tag="IWM52_002893"
     CDS             792..1895
                     /locus_tag="IWM52_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP9975067.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT          684 a          326 c          460 g          544 t
ORIGIN      
        1 gaatctctga agaataaatc acgtgatcgt gacgataaaa tacacttacg tcaggtgata
       61 aaaaatacat aagtagaaaa ctaagaggag gaacagaaaa tgaaagaagt tcaattgatc
      121 agaaaaagcg aactatcaga aggtggttgc aatgcatgcg gcgtagtaga agcaacaagc
      181 tatacattaa agcttggtgc caacaaagcg ataatctctg agttgacagt aggcggtctt
      241 gtggacagtc tggcgcttgc tgaaggtttt attggagaag atatttatga gatgttttca
      301 gaaatccgtc agttaaaaaa aggggaaaac tgcatagagg ttcatcatga gagcccgaat
      361 gtgagattca aacgtggtga taatgagatg atcttcaata atcatgtgtc cgatcacacc
      421 gagctatatg gaatcgtgaa tcaaatacta actgagcttt ttggattggg accttatgcg
      481 ttcaaggaag aaaacggtaa tccaaaatta aacgaagaat ggcaagaaac gattgaaata
      541 caaagaaaca acccgcactt atttcaatag gcatccacag tatttcgtaa aagggacttt
      601 gacacgactt ttgtcaaaat cccatcgttt tttatctatt tgtgttctaa acatttcttt
      661 cagagtagta acaactggaa tgactgaaat gtaaacagat tatttgcctg tatcaatacg
      721 gaagagtaat ataaagtgtg taaacgctat catattattc attcaatttt ttatagaggg
      781 aaggcattga aatggaaaaa acgtatcaag caggtacaaa tgaaggaata gtggatttta
      841 tcaatatgga agatttagaa atagccgctt cacaagtgat tccagctggt ggttatggtt
      901 acatcagcag tggtgctggc gacttattta cttatcaaga aaatgaacgg gcattcaatc
      961 atcagctgat tattcctcat gtattaagag atgtagaatt acccgataca actacacatt
     1021 ttgacaaaga aacgctgact gcaccaatca tcatggcacc agttgctgct catggcttag
     1081 ctcatgtgaa ggcggagaaa gcttctgcaa aaggtgtagc agattttggg acgatctata
     1141 cagcaagttc ttacgcttct tgtacattag aagagataag agaagcaggc ggggagaaag
     1201 caccgcaatg gtttcaattt tatatgagca aagatgatgg gatcaatttg gatattcttg
     1261 aagtggccaa gcgtaatgga gcaaaagcaa tcgtattgac agcggatgca acagttggcg
     1321 gaaaccgtga aacagaccgc agaaatgggt ttaccttccc actgccaatg ccgatcgttc
     1381 aggcttatca atcaggagta ggccaaacga tggatgctgt gtacaaatct tccaaacaga
     1441 agcttagtcc aaaagatgta gaatttattg ctgctcattc tgatttgcca gtctatgtga
     1501 aaggtgtcca gtcagaagaa gatgtctatc gttcattaga atctggtgct ggcggaatct
     1561 gggtatctaa tcatggtggc cgtcaactag atggtggtcc agcagcgttt gattcgttgc
     1621 aatatgtggc agaagcagta gataaacgtg taccaatcgt gtttgacagc ggtgtacgac
     1681 gcggacagca tgttttcaaa gcaatcgcat caggtgcaga tcttgttgct attggcagac
     1741 ccgttattta tggattgtct ttaggaggaa gtacaggagt ccatcaagtg tttgatttct
     1801 ttaaaacaga attggaaatg gtcatgcaat tagctggaac acaaacagta gaagatatca
     1861 aaaaaataaa attgcgtgag aatcgtttta tttaagaaac aaatgacgaa aaaaatagga
     1921 gtgcggcaga tgatgccgta ctcctatttt tatagttcaa ggatgtcact aatagaataa
     1981 tttgaaacga aaaaataaaa ttgaaatatt tttt
//
LOCUS       DACDYI010000156         2001 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.163, whole genome shotgun sequence.
ACCESSION   DACDYI010000156 DACDYI010000000
VERSION     DACDYI010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.163"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..376
                     /locus_tag="IWM52_002894"
     CDS             <1..376
                     /locus_tag="IWM52_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAP9975068.1"
                     /translation="NVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFRR
                     FPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTDD
                     WQKVMAAEVIATYLDGDLVYKK"
     gene            424..1824
                     /gene="yfcC"
                     /locus_tag="IWM52_002895"
     CDS             424..1824
                     /gene="yfcC"
                     /locus_tag="IWM52_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP9975069.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1857..>2001
                     /locus_tag="IWM52_002896"
     CDS             1857..>2001
                     /locus_tag="IWM52_002896"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP9975070.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          553 a          339 c          432 g          677 t
ORIGIN      
        1 aaatgtttcc acagaggact tctttaatta ttgtcgatta gaggaaagtg gtgtaacagt
       61 cactagcggt acagatttac cgctgtttat tactgatatt cctgaatcga ttataagagc
      121 agtattccgt cgttttcctg aaggagaaga agtctgggag aaagaaacaa gcttcacgac
      181 aaccgagctg ctgaaaagtt ttacaagcaa cagcttcaaa gtaaatggtt tatcggagta
      241 cggagaaata aaagtaggga aaagcgccag cttagctgtt ttcagcgagg acctctgtac
      301 tgatgactgg caaaaagtga tggctgcgga agttatcgct acctatcttg atggtgactt
      361 agtttacaag aaataatgct tttcgatata acgttataaa acgaattaaa gagaggttaa
      421 aaaatggaaa caatgaaaag aaaattcacc ttcccacata gttatacatt gatttttatc
      481 ttaattattt tggctgcaat tttaacttgg ataattccta gtggtcagtt tgagacagtt
      541 gttgaaaacg tcgcaggtat ggaaaaaaca acggttgttc cgggaaccta tcaccaaatc
      601 gataaggcag attatggaca aggtttttcc tctatactaa tggcaccagc tgaagggatt
      661 atttcagctg ttgaggttgt tgcttttgtt ctcatcgttg gtggtgcctt cgggattatc
      721 ctaaaaacgg gagccatcga tcgagggctg tttaccttag caaaagttct gggagacaag
      781 gggatcttag ttattccgat gtcgatgatt ctattcagct taggaggatc aacctttggc
      841 atgagcgagg aagtcattcc cttatttgcg gtgtttattt ctctgatgtt ttctcttggc
      901 tatgattcca tgactgcgat cttgattcta tttttaagta cacaagcggg atatgttggg
      961 tcgacgatta atcccttcaa cgttttaatc gcacagggag tagctggcgt ttacggaaat
     1021 cccctgcttt ggtttcggat gctatcctgg attgtcatta ctgggatttc aattgttttc
     1081 accatgcttt atgctagcag agtcaaaaag aatccactgt cctctattgt ttatgagagt
     1141 gatttaaagg ttaagcataa gtttcaaaat tttgacactg aaaaagagtt ttcaacacaa
     1201 gataaactaa ttatcgccgg ttttatcgta ggtttaggaa tcatgatttg gggtatcatt
     1261 tccaagggat ggtatatggt tgaaatttct gcactattct taggattagg tctttttgct
     1321 gggggcatcg ctagattttc tcaaaaggaa attgctgaag cttttgtcga aggctgttca
     1381 gatttcgcct atgctgctgt aatcattggg ctagctagag gagtcttggt aatcttggaa
     1441 ggtggaatga ttattgacac tattctaaac agcttggcta accttttagg tggtcttcca
     1501 aaattcctgt tcactacgat tctactcatt tctcaagtaa ttgtttcctt ttttgttcca
     1561 tcctcctcag gagctgcggc attaacgatg ccggttatgg caccattggg tgatctaatg
     1621 aatgtaagtc gtgatgttgt tgttctaagt aaccagtttg gaaatggttt aatgaatatt
     1681 atctccccaa ctagtggtgt attactggca ggattagcca tcgcagagat taatttctct
     1741 aaatggttta aggttggctt caaaatattt gccatcttaa cccttgtttc tgctgttttg
     1801 ttatacattg ctacactctt ttagtcttaa tcatccatta ataggaggca atagttatga
     1861 ttaaaataaa tcaattagca ggaatgaata tccactattt attttattct ctcaaagaat
     1921 ttttcgctta tcaacagaaa attggtattt cttctgttga actttgggga ggtgctccgc
     1981 attttttcat agatgctttt a
//
LOCUS       DACDYI010000157         1751 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.164, whole genome shotgun sequence.
ACCESSION   DACDYI010000157 DACDYI010000000
VERSION     DACDYI010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.164"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            383..1633
                     /locus_tag="IWM52_002897"
     CDS             383..1633
                     /locus_tag="IWM52_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP9975071.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     ETILEFNNSYHFDQIVIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFS
                     RMFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQH
                     FLENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGK
                     GRFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQ
                     VIVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQ
                     SQNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARK
                     FTRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          578 a          335 c          308 g          530 t
ORIGIN      
        1 ttgtctaata gtttttattc aaaatgtttc tagattataa aatttattga ataacacatt
       61 cctaacggaa tttgtttcga tattgtgtga tttctcagag tacaatggat gtatggaaag
      121 gatccaacgg gaatttgcta tttcctcctg tagcgtttgt ctaccctgtt tagtctgtta
      181 cccgaaccac tgttatcact accaactcat tagctgtttc gataatggac tagactttca
      241 aatagtagtt tggaaggcag cttctagctc ttttatcacc gtattctatt aggcgaggtt
      301 gtggctatac agtgagtttc tgggaaatcc cacagtatcg aagcattttc caaaatttat
      361 atcgaaagga ggtccttctt ccatgaaatt atttgtcggt attgacgtta gctctgaaaa
      421 actagatgtt tgttttttaa cagacgacaa tcaattgtct atcttatctg aaatctctgt
      481 tgccaatgac atcgaaggtg cttcattcat acgagaaaca attcttgaat tcaataacag
      541 ctaccacttt gatcaaattg tgattggcat ggagtcaacg tccatgtaca gcttccatcc
      601 ttccatgttt tttcatgaag atgagaaact aaagaagctc aacacacttg taacgattga
      661 aaatcctttt cgggtgaaac aatttagtcg catgtttgat gagaataaaa ctgatcgaaa
      721 cgatgcatta agaatcgctg atttcttgcg tattcaacga ttcacaactt cccctatcaa
      781 agaagagaaa tacatggcct tacagcgttt aacacgtact cgctatcaac ttattaagca
      841 attgattcgt acaaagcaac attttcttga aaatctaacg tacaaatgta atacgcttgc
      901 tcgagaaatg cgagacgaat cgacgagtct ttttagtgca accctcattt cactcatgac
      961 ggaagatttc acattggatg aacttgctga gcttccttta gaagctttct gtgatttact
     1021 tcaagaaaaa ggaaaaggcc gtttcaagca accagaaaaa atcgctaaag caattcaacg
     1081 tgcaattacc atgagttacc gcctaggtgc tcttgcacaa gaatcaatta atgttgtttt
     1141 aagtgttctc gtacgagaaa tacgagcact tgaaaaaaat atcaaagaat tagataaagc
     1201 catcgaacaa gtaattgttg tcattccaga atatcaatgc ttaaccagta ttccaggcgt
     1261 tggtaaagtc tatgctgccg gtttaatcgc tgaaattggt caaatcgaaa gatttgaaga
     1321 tcagacaaaa ttggcaaaat acgctggatt aagttggaaa gtgaaccaat ctggaaacta
     1381 tcagtcgcaa aatacacctc tgacaaaaca aggaaatcga tattttagat actacttagt
     1441 tgaagctgcc aactctgtaa aaaattatct tcctgaatac aaagcttttt atcaaagtaa
     1501 atataaggag gttccaaagc atcaacacaa acgtgcactc gtcctaaccg caagaaaatt
     1561 tacgcgtctg gtggatacgc tactacgtaa acaccaactc tatacgccac caaggagtgt
     1621 gatagagaaa taacaatccg ttattgacgc aaatccttga atcaacccga aaaaaatttg
     1681 attttgatcg ggtctagttt cgtgcgcttc aaaacatatt actcaataaa gaaaatctta
     1741 aatttcatct t
//
LOCUS       DACDYI010000158         1730 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.165, whole genome shotgun sequence.
ACCESSION   DACDYI010000158 DACDYI010000000
VERSION     DACDYI010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.165"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="IWM52_002898"
     CDS             complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="IWM52_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP9975072.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(1305..1709)
                     /locus_tag="IWM52_002899"
     CDS             complement(1305..1709)
                     /locus_tag="IWM52_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAP9975073.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          510 a          300 c          273 g          647 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcagatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgccaccagt tgtcagaaga
     1381 caaaaacttt tagaccagaa catttctttc caaagaaatt gtttgactct tggaaagtca
     1441 cgttttatca atcttgagct ggcactttta taggcattga tgaattttgt caattctgtt
     1501 ttaggttgag ctttgaacat aatatgaacg tggtctttat catgattcca ctccactaaa
     1561 gtaatgtgat acgattctga aattctttca aaagtagttt tggcataatc agatatttca
     1621 tcatcaatca cttgtctacg atatttcgtt actaaaacaa ggtggtaata aagaagaaat
     1681 actgaatgat tatttgtatc taattccatg ttatatcaac tcatttcttg
//
LOCUS       DACDYI010000159         1694 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.166, whole genome shotgun sequence.
ACCESSION   DACDYI010000159 DACDYI010000000
VERSION     DACDYI010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.166"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..596)
                     /locus_tag="IWM52_002900"
     CDS             complement(<1..596)
                     /locus_tag="IWM52_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9975074.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLG"
     gene            complement(607..1638)
                     /locus_tag="IWM52_002901"
     CDS             complement(607..1638)
                     /locus_tag="IWM52_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP9975075.1"
                     /translation="MIAELKPKHERQNFLVGDDRLDHAVAFLWKDPQTGETVGASYQG
                     TIVDFNRFGKRGTYKHIDKNPTPNHGFNLKIGDPKHLKFFESSIDLLSYAALNREKLQ
                     DAWLVSMDGLKHHVISHYVEESISELRRKQTFPQSIEICVDNDRAGHIFYEKEQMKGI
                     VDPFTNKKIRCERGIPNDWQVPKEYKATYEAVAKEMSVEPEAIMAIHKTETNLQLTNQ
                     LVSAHDVQSTFGKMLAKGEPVETIDLKEACTTVAKELKVCERADGTYNFDRFYSRKAN
                     IKDVNAGILLSYKAEQYYKGYKKHEHEFVPEVKKDWNDQLKHEIQQQEIRKQKRAMLF
                     QQGRQQERE"
BASE COUNT          423 a          379 c          287 g          605 t
ORIGIN      
        1 cctaaagaaa aacttcctgg tatgcctttt tgctgcaaga gtaaactata cagtgggcta
       61 ccgttcatcc caattaacca atcggcgatc tgacgtgctt gtacttcttc aaattttgga
      121 taggtctctt ctgcgtctcg taactgttgg aatccctgat aaatggcttc tttttctaag
      181 gagttgatcc aaagtctttt aaaggttttt ccttgattgg cgcctgcttg ttgaatgatt
      241 gaccaggcaa tcagctcccc ttcacgatcg ctatcagtcg cgatcacaat cgtatcagct
      301 gactgcagct gtcgtttgac gacactaaac tgctttttct tatctttagg aacatgatag
      361 tggtattgat tggggaaaat aggcaaatgt tctaatgccc attgtttcca tttttcatcg
      421 tacatatcgg gcgaatctaa ctcgactaaa tgaccaaaac catacgtgat cacagtttct
      481 cctgtaaaca atcgatcttg gatctcaaaa tacccatctt ttcgggtagc tttagaaaag
      541 ctatcggcat aagccttcgc ttgtgacggt ttttctgcta aaatgacggt tttcatagtg
      601 acgacttcac tccctttctt gttgtctgcc ttgttgaaac aacatcgcac gtttttgctt
      661 tctgatttct tgttgctgga tctcatgctt caattgatca ttccaatcct ttttcacttc
      721 aggtacaaat tcatgttcat gctttttata gcctttgtag tattgttctg ctttatagga
      781 aagaaggatt cctgcattaa cgtcttttat gttggctttt ctactataaa aacgatcaaa
      841 gttgtatgtt ccatctgcgc gttcacatac tttcagttcc ttcgcgaccg tggtacatgc
      901 ttctttcaaa tcaattgttt ctactggttc gcccttcgcc aacatctttc caaacgtaga
      961 ttgaacatca tgagcagaca ccaattgatt tgtcagctgt aaattagttt cagttttgtg
     1021 tatggccata attgcttcag gttcaacact catttctttt gcgactgctt cataagtagc
     1081 tttgtattct ttaggtactt gccaatcatt tggaattcct cgctcacaac ggattttctt
     1141 attggtaaaa ggatcaacga ttcccttcat ctgttctttt tcataaaaaa tatgtcctgc
     1201 acgatcattg tccacacata tttcaatcga ttgaggaaat gtttgttttc gtcttaattc
     1261 agaaatcgac tcttcaacgt aatgactgat tacatgatgt tttaacccgt ccatcgaaac
     1321 taaccaggca tcttgtagtt tctcccgatt caatgcagcg taacttaaca gatcaatact
     1381 actttcaaag aattttaaat gcttcggatc gcctattttt aaattaaatc cgtgatttgg
     1441 cgtaggattc ttgtcaatgt gtttatacgt tccccgttta ccgaaacgat taaaatcgac
     1501 gatcgttcct tgataacttg cccctactgt ctctcctgtc tgtggatctt tccataaaaa
     1561 agcaaccgca tgatctaaac ggtcgtctcc aactaaaaaa ttttgccgtt catgtttcgg
     1621 ctttagttca gcaatcaacc cttctttttc tgcataatca atcagccact ctggtagttt
     1681 tcgttcttcc ttca
//
LOCUS       DACDYI010000160         1685 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.167, whole genome shotgun sequence.
ACCESSION   DACDYI010000160 DACDYI010000000
VERSION     DACDYI010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.167"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..596)
                     /locus_tag="IWM52_002902"
     CDS             complement(<1..596)
                     /locus_tag="IWM52_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9975076.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLG"
     gene            complement(607..1629)
                     /locus_tag="IWM52_002903"
     CDS             complement(607..1629)
                     /locus_tag="IWM52_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP9975077.1"
                     /translation="MIAELKPKHERQNFLVRDDRLDHSVAFLWKDPQTKETVGASYQG
                     TFIDYERFGERGTYKHIDKNSTANHGFNLKIGDPKQLKFFESSIDLLSYAALNRDQLN
                     DTWLVSMEGLKHHVISHYFGEAVSELRKKQAFPQSIEICVDNDRAGHIFYEKEQLMGA
                     VDPFTNQKVRCERGIANDWQVPKEYKVIYEEVAKEMKVEPEAIMAIHKTENNLQFTNQ
                     LVSAHKVNASFGQQLSVNDSIEVISLKDICREVAKELKVCERSNGTYDFDRFYQGKGD
                     INAQILFSYKAEQYYKGYKNHEHEFVPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQG
                     RQQERE"
BASE COUNT          438 a          366 c          289 g          592 t
ORIGIN      
        1 cctaaggaaa agcttcctgg tatgcctttt tgctgcaaga gtaaactata cagtgggctg
       61 ccgttcatgc cgatcaacca atcagcgatc tggcgtgctt gtgcttcttc aaatttggga
      121 taggtctctt ctgcgtctcg taattgctgg aatccctgat aaatggcttc tttttctaag
      181 gagttgatcc aaagtctttt aaaggttttt ccttgatcag cgcctgcttg ttgaatgatc
      241 gtccaagcga tcagttcccc ttcacgatcg ctatctgtcg caataataat ggtgtcggcc
      301 gattgaagtt gctgtttcac cacgttgaat tgctttttct tatctttagg aacatgatag
      361 tgataatgtg tagggaaaat aggtaaatgt tctaatgacc attgtttcca attttcatcg
      421 tacatatcgg gcgaatctaa ctcgactaaa tgaccaaaac catacgtaat cacagtttca
      481 ccaggaaaca atcgatcttg gatctcaaaa tacccgtctt ttcgggtggc tttagaaaag
      541 ctatcggcat aagccttcgc ttgtgacggt ttttctgcta aaatgacagt tttcatcgtg
      601 acgatctcac tccctttctt gttgtctgcc ttgttgaaac aacatcgcac gtttttgctt
      661 tctaatttct tgttgctgga tctcatgctt caattgatcg ttccaatcct ttttcacttc
      721 aggtacaaat tcatgttcat gatttttata gcctttgtag tattgttctg ctttataaga
      781 gaaaagaatc tgagcattaa tatccccttt tccttgataa aaacgatcaa aatcgtacgt
      841 accattcgag cgttcacaaa cttttaattc ttttgcgact tctctacaaa tatctttcaa
      901 actaatgact tctattgaat cgttaacaga aagctgctga ccaaaagacg catttacttt
      961 gtgagcggaa accaattggt tcgtaaattg cagattgttt tctgtcttgt gtatggccat
     1021 aattgcttca ggttcaactt tcatttcttt tgcaacttct tcataaatga ctttgtattc
     1081 ttttggcact tgccaatcat tagcgattcc tcgttcacaa cgtactttct gattcgtaaa
     1141 tggatcaact gcgcccatta attgttcttt ttcgtagaaa atatgtcctg cacggtcatt
     1201 atccacacaa atctcgatgg attgaggaaa ggcttgtttc tttctcaatt cagatactgc
     1261 ctcaccaaaa taatgactga tcacatggtg ttttaaccct tccatagaga ctaaccacgt
     1321 atcgtttaac tgatcacgat tcagtgcagc atagctaagt aaatcaatac tactctcaaa
     1381 aaattttaac tgtttagggt caccaatctt taggttaaat ccatgattcg ctgtcgaatt
     1441 tttatctata tgcttatatg tgcctctctc accaaaacgt tcataatcaa tgaatgttcc
     1501 ttgataactc gcccctactg tctcttttgt ctgtggatct ttccataaaa aggcgaccga
     1561 atgatccaaa cgatcgtctc gaactaagaa attttgccgt tcatgttttg gctttagttc
     1621 ggcgatcaat ccttcttttt ctgcataatc aatcaaccaa tcgggcagtt ttcgttcttc
     1681 cttca
//
LOCUS       DACDYI010000161         1660 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.168, whole genome shotgun sequence.
ACCESSION   DACDYI010000161 DACDYI010000000
VERSION     DACDYI010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.168"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..1079)
                     /locus_tag="IWM52_002904"
     CDS             complement(<1..1079)
                     /locus_tag="IWM52_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP9975078.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIG"
     gene            complement(1079..>1660)
                     /locus_tag="IWM52_002905"
     CDS             complement(1079..>1660)
                     /locus_tag="IWM52_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP9975079.1"
                     /translation="QQQLVFFDIDSITGLDRGVQQCQMYMAMTLIWNQALIQGRRQRR
                     LIKEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQREMRKVEAMTVLATQSP
                     QEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEGLVGKSMTNSELESIPQQ
                     NQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
BASE COUNT          491 a          387 c          273 g          509 t
ORIGIN      
        1 atcccgatta cttgaccaac tttcacttga tcgccaacct tcacacgaga atttcctgta
       61 ctattcgcaa attcttgata taccatattc aaaccgtcgt aattaatcac gatcacacat
      121 gcacctaaac ctgaaatacc aggattgcca acgtacacga cttttcctcc atgcaccgcg
      181 tgaatttcac ttcctggatg atcaactgaa ccaaagtcta atccatcatg gaagccattc
      241 ggacggaatt caccgcctgg atttgttcca aataattgac caccgctgaa ggaactttta
      301 tctaaactgc tgccagggaa tggattcgtc cattcaccag taattgtgcc attgttattg
      361 ccaccttcac cccaatgttt gatggcgaat tcatcatatt cggttaattt attagaagtg
      421 ataattgaca tacaacctgt ataatacgga ctatcttcag taattcctgc agcggcataa
      481 ccgcctcgtc ctaagatcaa taaacttgcg ccagggcttt tatttccgac acaagcatta
      541 aaattggatt gtgtggctac cataaatccg taataggaaa atgcagcttt tgagttggga
      601 aacttcatgt accagccacc ttcaatacca ccgattcctc gcgcagttcc tttaggaaac
      661 agacacccat caaaccacgt aatcccaaaa tagttgagat catttcttgc ggacgccgac
      721 gtaccaaacg ctgattcgta accgtattgc gaaaggatca cacttggtaa taatttatat
      781 ttcactgctg cacctacggc atattgcgca atctcatcag aaaaagggcc tcttcctttg
      841 atttctttat aaatcggtaa gccttccgaa tattctcctg taaaagcttc gccaccactg
      901 cttgaatctg aatccgaatt atcaatcgag cccgccgcaa gtattagaaa tagggtcacg
      961 aacacagtga tcaaaatagt aatcaaacct gcagaagtac caaacaacca caaaaataag
     1021 cgccgctttg cacgacgctt aacatactgc ttgattaaac tttcattttg gttttccatt
     1081 attttcctcc atcaaacaat tttaattgac gtttgttcgg actaaaatga acacgaatgg
     1141 tttcttttga gccaaaattg atcacagcgt ctccttgatt ttgttgagga atggattcta
     1201 attctgagtt cgtcattgat ttacccacta atccttctat atgacgtaaa atagattcat
     1261 ccatacgcat aaagatcttc gtagaagaaa attcaaagac tttcttcagt ttcgagaagt
     1321 tatcagaaga catattttct ggcaccattt cttgtgggga ttgtgtagct aaaacagtca
     1381 ttgcttctac tttacgcatt tctcgttgaa agtttgtgac ataatttacc gtttcaataa
     1441 aatctggatt aatcgcatta tggcactcat caatcatcgc caagaagcgt ttgaagtctt
     1501 gttcttccat ttgtttttct ttaattaaac gtcgttgtct tcttccttga atcaatgcct
     1561 gattccaaat caacgtcata gccatgtaca tttgacattg ttgtacccct cgatctaagc
     1621 ctgtaataga gtcaatgtca aaaaagacca actgttgttg
//
LOCUS       DACDYI010000162         1609 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.170, whole genome shotgun sequence.
ACCESSION   DACDYI010000162 DACDYI010000000
VERSION     DACDYI010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.170"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(290..1330)
                     /locus_tag="IWM52_002906"
     CDS             complement(290..1330)
                     /locus_tag="IWM52_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAP9975080.1"
                     /translation="MTDFNFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCSVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFIPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAETESGISIIFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNSE
                     SFPKTITKDWLE"
BASE COUNT          511 a          243 c          212 g          643 t
ORIGIN      
        1 ggtttttagc ttttttaaaa aatgaaatta cctctcttcg cgtattatta taactaaaaa
       61 acagcaacat gcaaacgttt gttctctttt ttgtgttgga aattaattat taactattaa
      121 cacttatgac gtaaaaagct acgtttgtga tttttatcat aatattttaa ctcataaatt
      181 ctattaaaat agctatttct ttgtaacatt tgtaatataa ttagaataat aaaaaatttt
      241 taattttaat gctactccat gaagcaacaa tcaatttgtc agtgaaattt cactctaacc
      301 agtctttcgt aatagttttt gggaagcttt cagaattcaa acgtttttca cttgatactt
      361 tagcagctat tggtttttcg ataaaccaat tttttacata gacaaaaatc aggttctgga
      421 tattttcaac tttttcggtt tttaattttt gataggcttt taacagtgtc gtgtataatt
      481 cttgttctgc attgacataa taactatcga gttcttcaaa aattattgaa ataccacttt
      541 ctgtttcggc ttttttcttt gcattatgaa tagtctggac agttttttga gcttcactgt
      601 aacttgaaga aaatgttttt attaaattta atactcgttc agggataaac gtagcaatgc
      661 tgtcgtttgt cataattgtc tcaaaattgt ctaacaacaa ttgatcttgt aggcggtctt
      721 tttcagtgtc tatattgtgt ctattagtgt ctaatatatt attattaagt tctggtatga
      781 ttttcatacc ctctgattta tcaacgtttt ggcacggtat gattttcata ccctcttgag
      841 ccccaaggga ttcagcgtct gatttttcgt cgagggtggg tatgattttc ataccctctg
      901 atttatcaac gttttcccta ttctctctat caaatttttc gagtaagtag atatcatttt
      961 ctgagacttt taattctgct agatatagtc tattaggatt atttttccct aactgtttgt
     1021 taaatcccat ctgcttttgt aataataaac caaaggattc caactctttt ttgattgcta
     1081 tcgctttttg tttttggcag tttaatacgc tacatagttc atttgtcgta taagtaaaat
     1141 aaacattacc ttcttcatcg atttgccgtt tgctgattgc gatctctaat cttgctttta
     1201 aaagcatata ggcaatctta gttgaatctt tcattttttt gtattcgtca gaaacaataa
     1261 agacttttgg aaattgataa aataattcgt tgtaaactct atccgcctta aaaaaattaa
     1321 aatcagtcat tgtagatcct ctactttcac gttgttttat ttatgtaaaa ttacctactc
     1381 ttttcgaaaa tagcaaagat aaataaaaaa tgcaaacgca ttaaaactgt tttttaatta
     1441 aatactacct aaaatcagaa ataaaatgaa ttatgtacta cctttaaagt cattaagaac
     1501 tactttaaat aaatattatt tataattaaa tactaccaga agaacgtatg aataaaattt
     1561 agttttaaaa ttaaagataa ttaagtacta ccagtgtcaa aacaaaaaa
//
LOCUS       DACDYI010000163         1597 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.171, whole genome shotgun sequence.
ACCESSION   DACDYI010000163 DACDYI010000000
VERSION     DACDYI010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.171"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            481..1035
                     /locus_tag="IWM52_002907"
     CDS             481..1035
                     /locus_tag="IWM52_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9975081.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
     gene            1289..>1597
                     /locus_tag="IWM52_002908"
     CDS             1289..>1597
                     /locus_tag="IWM52_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9975082.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     L"
BASE COUNT          642 a          167 c          268 g          520 t
ORIGIN      
        1 aatattaatg aggtcattaa atcttattat ctttagtgat ataacgaaat atctacacag
       61 acaaaattat gatcttgcgt aaagttataa aatatcaata aaaatatttt attaatgaaa
      121 agcttggtag cacggttatt catatgctaa tttatttcac tttatagtaa ggggagttct
      181 tagtatattg ttcaaactta aatttcatat aatttaatta tgtaaattag ctattctaag
      241 aacttctacg gataattgcc cgtctaaaat ttaaataaga ggttagaatc ttcatataag
      301 taaggttaat ttttttgtac aaaaaagtcg gaatattctg agttactgaa atgatataaa
      361 atcataaact aaaccccttg attttcttca cttttaacag agtatatata taacttcatg
      421 attatggtaa aatatatata taatcatgaa ctcagaatat ttttaagaag ggaaacaata
      481 gtgagctata atgtacaacc gttaaggaca aatgaagaga tacaagattt tcagttttgg
      541 ctaagaagaa caagtaatcc agaaagagat agttttttat ttttgtttgg aatcaataat
      601 gggttacgta tgtcagatat tataggatta aaagttggag acatacgagg taaaagtaag
      661 cctattattg ttgaacataa aacaggtaag cgaaaaccga tttttataga taatttgaga
      721 gaagagattt tattatatac tgaaggtaaa gaagaaaatg attggttatt tccaagtcgg
      781 caacaaggtc gacatataac aagggatcgt gtctatcaga tatatgcaga tatagcagaa
      841 aaattaggaa gagatgatat aggaacacat acattaagaa aaacttttgg gtatcactac
      901 tacaagaaaa ctagagacat tgctacctta atgtttattt ttaatcatag ctcgcaagca
      961 attacaaaaa ggtacattgg tataactgaa gatgaaatag gggcatcgtt acgtggtttt
     1021 aaattgggag gctaattgag atattaggat atattttttg tgtataatga aaacttagaa
     1081 ggtgagatta atgaagataa ctgaattttc ggaagtattt aaaacgttta gtaacattta
     1141 caatagaata ccagagatag aacaaaatgt tgtagttgat aatgattatt atatagagaa
     1201 tgattttaat aaaccaagaa aatatgacaa cctatacaat tttatacaca atagaacaac
     1261 aataaataac aacagtaaca ctcgagtttt gattaattca gcatattttc atattaaata
     1321 tttaaagaaa tatgagtatg aagaagttgt attagattat gggctaaata gacagtcatt
     1381 tttaatttat gcaataaata acggaaattt gccaagcaat aaaaagaaca taattattga
     1441 aacatatgcc tatctggagg ggggatctaa aaaactaatt aagtcatgta atatagagta
     1501 tcaattatca gtaggagaaa ttaaatgtgt tttcgaagaa gatttagaac ttgacttaaa
     1561 aaaaattttt gccagcattc atgattatat aggtttg
//
LOCUS       DACDYI010000164         1583 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.172, whole genome shotgun sequence.
ACCESSION   DACDYI010000164 DACDYI010000000
VERSION     DACDYI010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.172"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            321..1055
                     /locus_tag="IWM52_002909"
     CDS             321..1055
                     /locus_tag="IWM52_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9975083.1"
                     /translation="MRSSKYTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVL
                     KGIEIGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFA
                     QLSEALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKS
                     AYLYDNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKAKGVTELAI
                     YQKGKRIMVPLQSKKTSSASKVRTFF"
     gene            complement(1052..1549)
                     /locus_tag="IWM52_002910"
     CDS             complement(1052..1549)
                     /locus_tag="IWM52_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9975084.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRIYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
BASE COUNT          509 a          271 c          247 g          556 t
ORIGIN      
        1 gccttccttt tttgtatgga ttttttcatt tacacaaaat attttatact ctcatattgt
       61 ttgctagatc caaactctta ttaaactttc cttgctataa aacccaaaca tagtaataaa
      121 agaataactt attaccttta aattatacta ccaacaaatg gtagtatatt attaatttat
      181 ataatatgaa acctaaaata tagtagtata cccgtggtgc tagttaattt tttcctagag
      241 gaaaatgtct aaacatgttg gtaaatcaac aaaaaaacgc atatagtagt ctcaaatctc
      301 ccaagaaatg aggactacat atgcgttcat caaaatatac tgaacatttt gacttagcca
      361 atcctgttac taaagtcgat gatatccccg attatgaaat gtacagtcaa acaatcgata
      421 gcttaaataa gcgttttggc aatcgtgttt taaaaggcat cgaaatcggt tacatcgctt
      481 ctgaaaagga tcggatcatc gactatttag ccgataaaga ttacgattta aaattactca
      541 gtgtgcatca taatggtcaa tttgattatt tagatgacga agtcaaagat atggatcctg
      601 ctattgtgat tcctcagtat tttgcacaac tttcagaagc actagttgtt atagaagcag
      661 atgtctttgc tcattttgac tatggttttc gggtatttgg tctatccgtt gctgaattta
      721 aacaatatga agcgcagttt ttaccgattc tagatcaagt aatcaaaaat aaattggcat
      781 tcgaattaaa tgctaagagc gcttacttgt atgacaattt agcgttatat gaatatgtca
      841 tcgatttata tctttcacgt ggtggtacct tgtttagtgt tggatcagat gggcattacc
      901 ttgaacattt ccgtttccat ttcgatgatc tttttgctct gttaaaagca aaaggtgtga
      961 cggaattagc gatttatcaa aaaggaaagc gaattatggt tccattacag tctaaaaaaa
     1021 cgtcctcagc atcaaaagtg aggacgtttt tttagatttc tttttctaaa atcggacaaa
     1081 tacgatcttg ttttcctaaa gggaaatgtt tgatcccgat ttttttaaat ccatgacgtt
     1141 cataaaaacg aatacctgca tgattttttt cataaacagt caaacgtaaa taacgttttt
     1201 gtttcgtttt agcataacga ttcgcttcat cgagtaaccg ttgccctaac cctttgtttt
     1261 gataatcttt gagcagatag atccgttgaa tctctatagc atctttttca tcattgactt
     1321 tcatataacc aatgagtatt tgatgatcct taataccaat aaatttactg gttgactgtg
     1381 ccaattctac tgccaatatt tcagttgtca attgtgtctg taaatatcct tgcatatcaa
     1441 gtggatctgc gccatcgata aacgattcat aaaaactgac aacacttaca acttgtaaag
     1501 catctagatc atcataatct aaatacgcta gtgtgatttg attgttcata taactcctcc
     1561 ttttgacata gtatactata aat
//
LOCUS       DACDYI010000165         1540 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.173, whole genome shotgun sequence.
ACCESSION   DACDYI010000165 DACDYI010000000
VERSION     DACDYI010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.173"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(2..334)
                     /locus_tag="IWM52_002911"
     CDS             complement(2..334)
                     /locus_tag="IWM52_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9975085.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            complement(384..>1540)
                     /locus_tag="IWM52_002912"
     CDS             complement(384..>1540)
                     /locus_tag="IWM52_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9975086.1"
                     /translation="NGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNER
                     GLKGTAEDLTEKAKDTLEEKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAY
                     GEGQVNAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKN
                     GQLPKTDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNS
                     ENEKRISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQ
                     FKDNDQSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAP
                     KPIQNVHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
BASE COUNT          374 a          304 c          254 g          608 t
ORIGIN      
        1 tttattcatc ctccttatac gtaccatgat aatttcggtt aggatctaac gcatgataat
       61 ggttcttgtc cgtaaaaaaa atagactcga taatcgcaaa aatttgtcgt tgacctgggt
      121 tattataggg tcgccaaaca atcactagag ctatcgcaat ccataagcca taattcaaga
      181 gtgcaaaaaa gagtggtaaa tggacaaggt tattgactat tttcgcccaa aaaaagcaac
      241 caccaagcgt taatagatcc aaaaagaaaa aggcgaaaaa cttccattcc ccactaaatc
      301 ttggagggtt tttcatgttt atatatttat ccataattga tttctccttt tcatataaag
      361 aaaaggccta acttgtaggc cttttatggt ttgatatcct gtttgatatc cctcaatagt
      421 tgttctttca atgcattgtt cctgttttta gtttctcttg tcacatttat ttttgttggt
      481 gcagtaaatg ccacatttgc tggcttgtaa cctgtatgaa cgttttgaat aggttttggt
      541 gcagcttctc ttgtcacaac attttgaacg gacgcattcc ctgtctcacc actttcagat
      601 aaattgtaag aaacagttgt cagatttcca ttttcaggca aacgacttgg tttcatttga
      661 acagtacctg caggtgattg atgaacaaaa ctttgatcat tatccttgaa ttgattcaaa
      721 cgactatctt tcattggtaa attttgtgcc ttaggtaaac caattttcgt tgatttttca
      781 gttccaactt cgattccatt atcatttggc agaacgatgg aatcctcatc cgtttgtact
      841 tcttgatttc gttttggtaa atctgaaatg cgtttttcat tttcggagtt aacagatcta
      901 taccgatatt gttctggatc agtttttggt aactgtccct cattttctgc tttgtcttct
      961 tgatttactt ttgataaatc tgaaattcgc ttctcatttt caaggtcact gttaggttta
     1021 tagcgatatt gttctggatc agtttttggt aactgtccat tttttccttc ttctgaattt
     1081 ctttcttcta tggaattttt ctcttcaatc gaattttcat tagagtgcaa cgcttcttgg
     1141 tgatctttga ctgaaggaat cactatacct tcttgatttt ctttttcggt tgttgtatta
     1201 gcttttactg ctgcattaac ttgtccttca ccatacgcat cttttagtcc ttctttgact
     1261 tctgaagctt tttctttttg tttttctagt gcttcggatg gattcttcac agcttcagcc
     1321 aattctttag ctctcgtgcc gtctaatttc tcttctaagg tgtctttggc tttttctgta
     1381 agatcttcag ctgttccttt taacccacgt tcattgacgt aaccaagacc ttctgcggct
     1441 ttagaaacac catttttcgc tttcccgaac atttcggaaa ctgttttctt actattaggt
     1501 acagttgtag ctttatcgtc tgattgttct tccccgttat
//
LOCUS       DACDYI010000166         1510 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.174, whole genome shotgun sequence.
ACCESSION   DACDYI010000166 DACDYI010000000
VERSION     DACDYI010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.174"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..1503)
                     /locus_tag="IWM52_002913"
     rRNA            complement(<1..1503)
                     /locus_tag="IWM52_002913"
                     /product="23S ribosomal RNA"
BASE COUNT          316 a          455 c          295 g          444 t
ORIGIN      
        1 atcgtctgca ttccttagcc tcctgcgtcc ctccattgct caaacaaaaa caacgggtac
       61 aggaatatca acctgttatc catcgcctac gcctgtcggc ctcggcttag gtcccgacta
      121 accctgggcg gacgagcctt ccccaggaaa ccttagtcat tcggtggaca ggattctcac
      181 ctgtctttcg ctactcatac cggcattctc acttctaagc gctccagcag tcctcacgat
      241 ctgccttcaa cgcccttaga acgctctcct accaatacac ctaaaggtgt actccacagc
      301 ttcggtaata tgtttagccc cggtacattt tcggcgcagg gtcactcgac tagtgagcta
      361 ttacgcactc tttaaatggt ggctgcttct aagccaacat cctagttgtc tgtgcaaccc
      421 cacatccttt tccacttaac atatattttg ggaccttagc tggtggtctg ggctgtttcc
      481 ctttcgacta tggatcttat cactcacagt ctgactcccg gatatgaatg aatggcattc
      541 ggagtttatc tgaattcggt aacccgagat gggcccctag tccaaacagt gctctacctc
      601 catcattctc aattccgagg ctagccctaa agctatttcg gagagaacca gctatctcca
      661 agttcgtttg gaatttctcc gctacccaca cctcatcccc gcacttttca acgtacgtgg
      721 gttcggtcct ccagtgcgtt ttaccgcacc ttcaacctgg acatgggtag atcacatggt
      781 ttcgggtcta cgactacata ctcaaacgcc ctattcagac tcgctttcgc tgcggctccg
      841 tctcttcaac ttaacctcgc atgcaatcgt aactcgccgg ttcattctac aaaaggcacg
      901 ccatcaccca ttaacgggct ttgacttgtt gtaggcacac ggtttcaggt tctatttcac
      961 tccccttccg gggtgctttt cacctttccc tcacggtact ggttcactat cggtcactag
     1021 ggagtattta gccttgggag atggtcctcc cggattccga cggaatttct cgtgttccgc
     1081 cgtactcagg atcctcctag gtgtcttcca catttcgtct acggggtttt taccctcttt
     1141 gactgacttt tccaagtcat tcgactatct gaaagaacta ccatattgga gtcctacaac
     1201 cccaacaagc aagcttgctg gtttgggctt ttcccgtttc gctcgccgct actcagggaa
     1261 tcgaattttc tttctcttcc tgcaggtact tagatgtttc agttctctgc gtctacctcg
     1321 aatgtgctat gtattcacac aatcgtaaca tcctattaaa gatgttgggt tcccccattc
     1381 ggaaatctct ggatcatagc ttacgtacag ctccccaaag catatcggtg ttagtcccgt
     1441 ccttcatcgg ctcctagtgc caaggcatcc accgtgcgcc cttattcact taaccttatc
     1501 aaccttacgg
//
LOCUS       DACDYI010000167         1502 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.175, whole genome shotgun sequence.
ACCESSION   DACDYI010000167 DACDYI010000000
VERSION     DACDYI010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.175"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(69..224)
                     /locus_tag="IWM52_002914"
     CDS             complement(69..224)
                     /locus_tag="IWM52_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010001600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9975087.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
     gene            841..1062
                     /locus_tag="IWM52_002915"
     CDS             841..1062
                     /locus_tag="IWM52_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9975088.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            1063..1320
                     /locus_tag="IWM52_002916"
     CDS             1063..1320
                     /locus_tag="IWM52_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9975089.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKAAKSAVPTGFLTKQPYIF"
BASE COUNT          507 a          340 c          238 g          417 t
ORIGIN      
        1 atgctataat caccttaatt gaacaacagt ctaacagcaa aagcagtgcg ccaacactaa
       61 tgcttttgct attttttttg ctcattttcc tttccgttag cattaaccca aattgtaata
      121 agaccagact tactaagacc catttgtttc gcaattttat caagattatc tgccacttcc
      181 ttagtaagcg ttacaacgac tctctttttt tcattactga tcataattcg tatcctccaa
      241 cgactttttc ttaaaaacca tagtaacaaa aaagtaacta aggtgcaaca gtttagatac
      301 taattagcga ataataggcc ttaaaccctt ggcattactg attttgtcaa aaaaaaacgc
      361 tgaaaccctt gggagagtaa ggctaaacag ctatcgacca atgaccaatg accaataagg
      421 ggggtgtcgt agtccccccc tcctccgccc aattcataaa caccaaaacc aaaaaacaaa
      481 cctcagggct ccgccccgaa ccccgacctc cttctaccga aatatatgca ggcgccacgc
      541 ttactatttc gttcagctat cggcttttgc aatctaacac gaccgtttcg ctaccagtcg
      601 ccttacaggc tccattctcg cttctcttac gtgttttaac gattgcgccg ataactaaac
      661 gaaatagacg ctaaatcgct taaaacgcaa aatagaccct ttttagagca ttgatttttt
      721 ctactagtca tttcgataat ataaaattga ccacaacaca gaaaacttat attataagct
      781 tatgtcgtaa gtccgacgta ctcacgtaat atagacggac gttaaatgga aggagcataa
      841 atgagagtga taaaatcaga aaaattcact ttggccgaaa ttatgacgac cagcgaactt
      901 gcacaagaac ttgattacaa ccaagcttac gttttgcgct tagcgaaaga acatctgact
      961 gaaggaaaag aatatcgctc tgccggtaga agaaactatc ttttcagccc agaagccctt
     1021 aaaaaactag aaaaagtcat aaccaccaaa gaaggtgagt aaatgcctaa agaaacaact
     1081 atcaacgttg aacaactaga aaaattgcta gaatcatatt ccaatgattt tatcgaattg
     1141 accggcggaa aagtcgaaga aaccaccgac ctctttgacc tacttctcac aaatattctg
     1201 gaattaaact ctgaagaaca aaaagagcat aagggaaaac tgatagcacc ttcctacagg
     1261 aaggcggcta agtcggctgt gccaactggt tttcttacaa aacaacctta tattttttga
     1321 tatcacaaaa aaatccattt tggacctatt ttcggtcata atatagtacc tacttttggt
     1381 catagtttcg tccgtagttt tggtcatagt gactaagact ttcacgcaca aaaaaagagc
     1441 tgaaattaac agctcttatc ctttatttat ttttgaattt acacttccac catgacgatt
     1501 cg
//
LOCUS       DACDYI010000168         1496 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.176, whole genome shotgun sequence.
ACCESSION   DACDYI010000168 DACDYI010000000
VERSION     DACDYI010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.176"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(139..1158)
                     /locus_tag="IWM52_002917"
     CDS             complement(139..1158)
                     /locus_tag="IWM52_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9975090.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1169..1375)
                     /locus_tag="IWM52_002918"
     CDS             complement(1169..1375)
                     /locus_tag="IWM52_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP9975091.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          415 a          311 c          254 g          516 t
ORIGIN      
        1 aacaaacctg ttatcgcata gcttggagtg tgactattat gggtgatagt gaagatatgc
       61 gataacatca ctattttatc gaatgatttt tatagttgtc aatatagtta tgtaatgctc
      121 ctcaacgagg agctattttt atcgtttagg aatatttaaa taccaacgtt tgtcatggaa
      181 atcttgcgca ccgcctttag tgtttccctc tggatcattc gttgcccgca tcatgacata
      241 gactttctta ttagggaagt tacgcatgtt aaaagataca tgatagccaa catttccaga
      301 agtattataa gcttgattta catctgatct ataaattcca tcagctctta ctcgagctaa
      361 ttctttccca gtattgtaat ccataatgaa gatatactcg tatttatagt tagcaatgtg
      421 ccatccagct acatgcaaat ttgcattttc gatttctcca aactgatcaa tgtgggcgta
      481 attcgttcca tctgtcagtg taggattagc tgcaccagct cgtgttggat caatgacagg
      541 cttgttttca gaagttgttg gattttcatc ggtaaatcca tgagctaaat catatgctaa
      601 tttttcttta cttacgccca tttcagaaag ataaccgtaa ggatctgtat gatcgcccca
      661 gatgttttgt gttacccata aatgcgattt gattcccggt tggttataag gagtgtccaa
      721 cgttaatgga ataccatatt tcattgctga atctctagcc aattcaacgt atgccttgta
      781 gtttttctca aacgttgctt tatcatgtgt gtgttgtaac tcaatctgca caggactgtt
      841 ggcattagca tacgaaccag caccgtactg tacataacca ggttgaccga cttgataaac
      901 aattccgccg tctcccacaa tataagcagt ataagcgcta gtccatgaac gttgcatata
      961 ctgcgcttca ttgcgtcctg ttgctgtttc attagccgtt tcatgcagta aaatatactg
     1021 attatttgct acttgtgagc taccttcatt tgcgcccaaa ttaaattcat tgttgatagt
     1081 ataggcaaat ccgttaattg gcaataaaaa aagagccgtt aacaggctca tcgcagtaat
     1141 agtaattttc tttttcatat ctatcctcct atttatctga tgaaattttc ttatacgttc
     1201 gatttgatac acctaaaata ctccctagaa atgctccaaa acctgtaata atcatcaccg
     1261 ttatatctgt gtaccgccag ttaacacctt ttccgacaag accgacaaaa gtcgctaaag
     1321 cggggataac tactatagcg atccatttca aaatatcaaa ggttctattg ttcatactat
     1381 ccacctccct tcattggttg ggctactttc tttttcaatc gcttgttttc ttctatcaat
     1441 atttctcttt ctgaaagcaa ggcatcaatt tgcccgtcca gttgttcaat tttatc
//
LOCUS       DACDYI010000169         1470 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.177, whole genome shotgun sequence.
ACCESSION   DACDYI010000169 DACDYI010000000
VERSION     DACDYI010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.177"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..213)
                     /locus_tag="IWM52_002919"
     CDS             complement(<1..213)
                     /locus_tag="IWM52_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9975092.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIE"
     gene            complement(398..796)
                     /locus_tag="IWM52_002920"
     CDS             complement(398..796)
                     /locus_tag="IWM52_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAP9975093.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(793..>1470)
                     /locus_tag="IWM52_002921"
     CDS             complement(793..>1470)
                     /locus_tag="IWM52_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP9975094.1"
                     /translation="NIISFRMRKKRFFDIMKGFLITTIGLILFLHGVNMAYVPVGQHL
                     GSAIAGLPYNYILVPLGFLMGFLVGFAEPAIHVMIKQVEELSEGRIRSKIMLMVISIG
                     IGAAVGLSMWRLLVGFSLYYFLIPGYGLAFILGRKVDKIFLAMAFDNGGVATGPMCST
                     FILSMSVAIAAQIEGRDPIIDGFGVVAMIALTPILSTLVLGYIYKRKDEKDRIEKERL
                     QKLGESK"
BASE COUNT          457 a          342 c          210 g          461 t
ORIGIN      
        1 ctcgatataa tcttttactg gtaattccaa ttctacctta aaaaagtgca aaacagccga
       61 cagcccttct gcttgataga tttctcctaa tgttagcccc tctagatgga actctggtga
      121 aaaatgcagc aatgttttat tttcctgcaa ttgaacatat agaaatgtag atcgctcaat
      181 cagagtatct tttaaaacaa ctaaaaaatt catttttctc cccctttttt catcattttt
      241 aaaatagcat attttgaaaa gaaagtctga tgcttagagt agaatttcat aaaaatttac
      301 tatttcataa gtattcacga gaaaaataca aacaaaaaag cggaaataac gctcatccgc
      361 cttcttgttt gtgtttattt cctcttcaaa cgcttgtcta tgattcagac ttttctctat
      421 cgttattttc atctaaaagt tcttgcattc cgataatttc tcgatattga tgaatcttag
      481 tgataccaat gactttgtct acatcaatac taaaacaaat accattacct ggaatatcga
      541 tgttgatgcc ttgaccaatc gcatccatta ctttattcgt caaactatcg ggtactaatg
      601 tcaacacaat atctttttct ggcgaaatgt ccaaacctaa aaatttccct gaatcatgta
      661 caccagaacc tcttccatgt aagatcgttc cgcctgctgc acctgcttct tttgaaaagt
      721 cgataacatc ttcgcccatt cctccgtcaa cgatcgtcac aatcatgtga agattcaatg
      781 ctacaagatc tttcatttgc tttcccccaa tttttgcaat ctttcttttt cgattctgtc
      841 tttttcatct tttcgtttat aaatatatcc tagtactaaa gtggataata ttggtgtcaa
      901 tgcaatcatt gcaaccacac caaacccgtc aatgatcgga tctcttcctt caatttgtgc
      961 agcgattgcc acgctcattg ataaaataaa tgtcgaacac atcgggcccg ttgctactcc
     1021 accgttatca aaagccattg ctaaaaagat cttgtccact tttcgtccta aaataaatgc
     1081 tagcccgtat cctgggatca agaaataata aagagagaat ccgacgagca aacgccacat
     1141 agacaatcct actgctgcgc cgatcccaat tgaaattacc atcagcatga ttttcgatcg
     1201 gattctcccc tcgctcaatt cttcaacctg tttgatcatt acatgaatcg ccggctcagc
     1261 aaaccctact aagaacccca ttaaaaagcc aaggggaacc agtatataat tgtaaggcaa
     1321 tcccgcaata gcactgccta aatgctgtcc taccggtaca taagccatat tgactccgtg
     1381 caggaaaagg atcaacccaa tagtcgttat caagaaacct ttcataatgt caaagaaacg
     1441 ctttttgcgc atacgaaaac taatgatatt
//
LOCUS       DACDYI010000170         1457 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.179, whole genome shotgun sequence.
ACCESSION   DACDYI010000170 DACDYI010000000
VERSION     DACDYI010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.179"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            109..1041
                     /locus_tag="IWM52_002922"
     CDS             109..1041
                     /locus_tag="IWM52_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAP9975095.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1113..>1457
                     /locus_tag="IWM52_002923"
     CDS             1113..>1457
                     /locus_tag="IWM52_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9975096.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT          640 a          169 c          228 g          420 t
ORIGIN      
        1 gatgaaataa atcaaaagta tgatgaaaca ttaaaaaatg tctattcttc acttatcgtt
       61 tcaaatatag aagaaacttc atcttctgaa ataaaaaata agctttcttt gttaaaatta
      121 aaagaaaagg aatatattat tgacgatgct gtttctgtcc ctcttatttt aaataaacga
      181 gagaaaaata atttaaagaa aaaaatgctc attccatttg attcagaagt gaaaggatta
      241 ctcaataaac taactatttc taatatagac tcgactaggg aaaaaatcat aaaaatattt
      301 gataaaatta ataatttatt caagaatgac aatgctgaat taaggaaggg tcttttagag
      361 tttaaacttc aagaactaga acttcattac tcttatttag tgaaaattac cgaagaaaaa
      421 gaacaacaaa aagcaataaa agaacagatg atagaagagg aaaaagttcg tagagaaatt
      481 gatcgtgaaa agaaacgtat tgataaagaa caacgtcaat tcaattctga aatttccaaa
      541 ttaatcgcat atatgcaaaa agctaatgca gatgtggaaa aagaattata tgcaaataaa
      601 attcaagaac tggaagaaaa attaaaagaa ttagaagtta tacgtgaaaa tgtattacaa
      661 agagaattaa atactagagc cggttatgtg tatgtaatta gtaatattgg gtcttttggt
      721 gaagacatat ataaaattgg tatgactaga agattagaac ctatggatag aataaaagaa
      781 ttgagtagtg catcggttcc atttgaattt gacgttcatg cgatgatttt ttcagaggac
      841 gctccgtctt tggaaactaa attgcataat cattttagaa aacaagaagt taataaaatt
      901 aatcaaagaa aagaattttt caaagtttca ctagacgaaa tagagaaagt tgttttagaa
      961 aattataatg gaacagtaac atttacaaaa ttagctaaag ctgaacaata tagacgatct
     1021 ttagaattgt caaaagatta aatatataaa aagccttcgg gctttttctt ttaacaatta
     1081 ataggaacgt atattcgaaa ggaatagaaa taatgtggtt agaaaaaata aatgacaaaa
     1141 aatttaagta caccgaacgt tatacggatc ccttaactga aaaaaagaga aaagtgtccg
     1201 ttactttatc tagcaattca cgccaagctt ggaatcaagc taacttactt ttgaatgaaa
     1261 aaatcgcaga aaaaataaaa cgaaatgaag aattaccact aacttttgga gaattaaaaa
     1321 ctaaatggga tgaaaaatac aagccaactg taaaagaaag ttcctatcga acaactcagg
     1381 tttatctatc attaatttca aaatatataa aagatgatgt acttgtaaaa aacgttaata
     1441 gtaatcttat acaagat
//
LOCUS       DACDYI010000171         1424 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.181, whole genome shotgun sequence.
ACCESSION   DACDYI010000171 DACDYI010000000
VERSION     DACDYI010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.181"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(150..1337)
                     /locus_tag="IWM52_002924"
     CDS             complement(150..1337)
                     /locus_tag="IWM52_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAP9975097.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DACDYI010000172         1421 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.182, whole genome shotgun sequence.
ACCESSION   DACDYI010000172 DACDYI010000000
VERSION     DACDYI010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.182"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            96..1391
                     /locus_tag="IWM52_002925"
     CDS             96..1391
                     /locus_tag="IWM52_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9975098.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          450 a          281 c          266 g          424 t
ORIGIN      
        1 cttcaccaac atcttttttg tataaaaaaa tatagaaaag acaccctgaa atcctttaga
       61 attaatttgg ctaccaattc ataggaggat tcaagatgtc tcatactcag cttatcaaag
      121 ataccctaaa tattctagac ctaaatatct atttcgagga aaattgttta actaaagaaa
      181 aatacaaagg gcaaatttgc ttgatttaca aaggaactct tctgtacaag ccagaagagt
      241 gttcccattg tctttgtgtc gtcccctcta ggatcattcg ttgggggacg acaaccgttc
      301 ggttgttact gaatgatgtg tctgagtatc gcacttatct ggagctgaag aaacaacgat
      361 ttaaatgtaa gtcttgccaa agaacattcg tagcggatac ctctgttgct gaaaaacact
      421 gtttcattag tcaaaaagtt cgttggtctg tcattgctcg cttaaaagag aatacttcca
      481 tgacggaaat cgctcgtcag aaaaatattt ccacttcttc ggtctatcgt gtcatgaagc
      541 gattctaccg cccattgaat cctttcaaac agactttgcc aaaggtctta tgctttgatg
      601 aattcaaatc ggttcgaagt gttgccagtg caatgagttt tatcatgatg gacggacaaa
      661 gccatgcatt attggatatt gtcgaaaatc gacgcttgcc ttatttagaa cgctattttt
      721 ctcggttttc attagcgact cgtgaagccg ttcaattgat tgttattgat atgtatgcgc
      781 cttatgtttc gttggtgaaa aagcttttcc cgaatgctca gttgatcatc gatcgtttcc
      841 acatcgtcca acatattggc agaactttct tgaatcatcg agtgaaagaa accacggtac
      901 gacttaaagg aaattcccac tctcaacgag ggttagggaa aaaattgaag cgctattgga
      961 aactcctaca aaaagaagaa gcaaagttaa actatgaaaa acgggtttgg cgtgccagtt
     1021 ttaaagatta tttgactgaa agtgagattg ccgatcgact gttagctgct tgtcccaact
     1081 tacgccaagg ctatcagtta taccaagaca ttctttatgc ggtaaagaaa agagatcagt
     1141 cactatttga ggattgttta actagagaca ttagcggtct gccagaaacc tatacaacca
     1201 ctttacgaac gtttaaaaag tttctgccac agatccaaaa tgcgcttcac tacagctatt
     1261 caaacggacc attggaatgc ttaaacaacc acatcaaagt attaaaacgc aatgcgtacg
     1321 gctttcgcaa cttttacaat ttcaaactac gaattatgat tcgtcatggc aaaacatttt
     1381 taaccaaata aacagaacag aaagaggttt tcttctttct g
//
LOCUS       DACDYI010000173         1385 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.183, whole genome shotgun sequence.
ACCESSION   DACDYI010000173 DACDYI010000000
VERSION     DACDYI010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.183"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..1307
                     /locus_tag="IWM52_002926"
                     /note="aph(2'')-Ia"
     CDS             <1..1307
                     /locus_tag="IWM52_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HAP9975099.1"
                     /translation="LKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYP
                     KTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRA
                     YQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSI
                     EIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYS
                     YISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECT
                     IDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNH
                     LLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKA
                     KEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          595 a          103 c          233 g          454 t
ORIGIN      
        1 cattaaaaaa acattataca gagccttggg aagatgaagt ttttagagta attattgaat
       61 ataacaatgt tcctattgga tatggacaaa tatataaaat gtatgatgag ttatatactg
      121 attatcatta tccaaaaact gatgagatag tctatggtat ggatcaattt ataggagagc
      181 caaattattg gagtaaagga attggtacaa gatatattaa attgattttt gaatttttga
      241 aaaaagaaag aaatgctaat gcagttattt tagaccctca taaaaataat ccaagagcaa
      301 taagggcata ccaaaaatct ggttttagaa ttattgaaga tttgccagaa catgaattac
      361 acgagggcaa aaaagaagat tgttatttaa tggaatatag atatgatgat aatgccacaa
      421 atgttaaggc aatgaaatat ttaattgagc attactttga taatttcaaa gtagatagta
      481 ttgaaataat cggtagtggt tatgatagtg tggcatattt agttaataat gaatacattt
      541 ttaaaacaaa atttagtact aataagaaaa aaggttatgc aaaagaaaaa gcaatatata
      601 attttttaaa tacaaattta gaaactaatg taaaaattcc taatattgaa tattcgtata
      661 ttagtgatga attatctata ctaggttata aagaaattaa aggaactttt ttaacaccag
      721 aaatttattc tactatgtca gaagaagaac aaaatttgtt aaaacgagat attgccagtt
      781 ttttaagaca aatgcacggt ttagattata cagatattag tgaatgtact attgataata
      841 aacaaaatgt attagaagag tatatattgt tgcgtgaaac tatttataat gatttaactg
      901 atatagaaaa agattatata gaaagtttta tggaaagact aaatgcaaca acagtttttg
      961 agggtaaaaa gtgtttatgc cataatgatt ttagttgtaa tcatctattg ttagatggca
     1021 ataatagatt aactggaata attgattttg gagattctgg aattatagat gaatattgtg
     1081 attttatata cttacttgaa gatagtgaag aagaaatagg aacaaatttt ggagaagata
     1141 tattaagaat gtatggaaat atagatattg agaaagcaaa agaatatcaa gatatagttg
     1201 aagaatatta tcctattgaa actattgttt atggaattaa aaatattaaa caggaattta
     1261 tcgaaaatgg tagaaaagaa atttataaaa ggacttataa agattgatta tataatatat
     1321 gaaaagctat tataaaagac attagtatta aatagtttaa aaaaatgaaa aataataaag
     1381 gaagt
//
LOCUS       DACDYI010000174         1311 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.184, whole genome shotgun sequence.
ACCESSION   DACDYI010000174 DACDYI010000000
VERSION     DACDYI010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.184"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            200..1248
                     /locus_tag="IWM52_002927"
                     /pseudo
     CDS             200..1248
                     /locus_tag="IWM52_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180785.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
BASE COUNT          532 a          148 c          254 g          377 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tcttgatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagatgg aatggcaaca
     1261 ctttttttga caaaaattat aaggtgttgt gaatgctgcg gtgttatggc t
//
LOCUS       DACDYI010000175         1297 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.185, whole genome shotgun sequence.
ACCESSION   DACDYI010000175 DACDYI010000000
VERSION     DACDYI010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.185"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            141..398
                     /locus_tag="IWM52_002928"
     CDS             141..398
                     /locus_tag="IWM52_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9975100.1"
                     /translation="MSKLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(630..1043)
                     /locus_tag="IWM52_002929"
     CDS             complement(630..1043)
                     /locus_tag="IWM52_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HAP9975101.1"
                     /translation="MDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVLSASIPI
                     LIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEKLLYITK
                     TSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          492 a          169 c          161 g          475 t
ORIGIN      
        1 ttctgtccac gttcatatta ctccaaagtt ttcctcattc caaaagaaaa gcacagaata
       61 cacaaagaag cgtttttatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaaat tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctgata aatatgaaaa atatgatgaa aaagaacttg
      361 acaaagaatt taaagaaatc aatgacttat tactataatt gatttatacg actcattttt
      421 gagtcgtcag actgcaaaca acactctaaa tttaggagtt tgtctacagt ctgaataaat
      481 tttaaattta ttgtccataa tgttattatg aatttatagc attaagaaaa gagataagac
      541 tttacatttt tttaggaatg taaagtctta tctcttttct taattgtaca attagtatta
      601 tctgcactac tttctaatta aataaatttt tagttattaa cttgattaaa atcagagata
      661 atatcattta attcagtttg aagggttgta acgattaaat ggaaattttc ttcatctgtt
      721 ttattatatg gggatgtttt cgttatatat aacaactttt ctttttcaat tttcattaat
      781 aactctgtcg aagtctgtac tttatcttga taattaaatg cagacatccc actttgtata
      841 attgtaatta ttgctgaagc aattgaaaca atcaatagta aagacctgtg ttcggaagcc
      901 tgatcaatta atattgggat actagctgat aagactattt ttataatatt tccgatacca
      961 attactctat tataaaatcg aatttctttt tttaatctat cgattgtaga atcaatagat
     1021 tcgataaacg ctttttgatc catcattttc tctccaatca ttttattaaa atcattaaca
     1081 aaaatattat aataggattt aagtaagttc taactaatca cacataaaaa taaaaaacat
     1141 ttgtatttta tcaaagtttt attatacttg tcaatttgtg tacatcctaa aatataagct
     1201 atttgtttac ttctgtagaa tatatacttt gttgttagat aagttgcaca tgtttataga
     1261 atctaaataa taaattttta tgtaccgccc ctccaaa
//
LOCUS       DACDYI010000176         1283 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.186, whole genome shotgun sequence.
ACCESSION   DACDYI010000176 DACDYI010000000
VERSION     DACDYI010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.186"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..267
                     /locus_tag="IWM52_002930"
     CDS             <1..267
                     /locus_tag="IWM52_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory protein"
                     /protein_id="HAP9975102.1"
                     /translation="DYFLPDIYARRLYHNMHDQIEEEAKENIFIKIVQSLDNSTAQKL
                     LIQFYEEKQIDRLQEYLEKLRQNEEITEVIRKRLLLEAEKAVKE"
     gene            264..>1283
                     /locus_tag="IWM52_002931"
     CDS             264..>1283
                     /locus_tag="IWM52_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9975103.1"
                     /translation="MIIGGIFSGIADWWSGLFGDGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKIKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLENDPNKVEHIPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSF"
BASE COUNT          443 a          184 c          247 g          409 t
ORIGIN      
        1 gattattttc tacctgatat ctatgcacgt cgactttatc acaatatgca cgatcaaata
       61 gaagaggaag ctaaagaaaa catttttatt aaaatcgttc aatctttaga caactcaacg
      121 gcacaaaagt tacttattca attttatgaa gagaagcaaa tagatcgttt gcaagaatac
      181 cttgaaaaat tgcgtcaaaa tgaagaaatt acagaggtta taaggaaacg acttttgcta
      241 gaagctgaaa aggcggtgaa agaatgatca ttggaggaat attttccggc attgccgact
      301 ggtggtcagg cttatttggg gatggggatg cagatcccaa aaccgtaacg aaagtcttac
      361 aagattatgg taattatctg cagtatcaag atgtattcag ttggatttgt aatacttttt
      421 tatggggttt gattaaaggg ttatataatc tcaatacttt actagaaaaa acaatttatc
      481 aaagctttga tttaaaagac ttattgaacg ccgctggtgt taatgaactg tttcaaagtt
      541 tgattagtaa ggtattggct cttttttgtg tcgtcacatt aatttattta ggcttaaaat
      601 tttttatgag taaacatcca ccaaaaatca aaaatatctt ggttaatcta tttatggcca
      661 tgattttgat ttttggtggt agttctttaa tcgatgaagg gctaaatatt agtaaaaact
      721 tttacggaga cgtgactgca gcaaataaag cagataaaac tggttcacct gcttttcaaa
      781 ttattcaaaa taatgtgatt gatgtgagta cgttactaga aaatgatccg aacaaagtag
      841 aacacatacc aacagacaaa agaaatgctc tcacggcgaa aaattttaaa acagcgaata
      901 tcaatgggat tattacacct gatcaagcta agaaaatgtc tgaatcaagt gatattagta
      961 cagtacaaaa agatcggtta aaggcattac agtatcgact agagttagac gataatggga
     1021 aagaaatccc tgttcaaatt agtgatgaag gtttagcaaa atatgtgtat acttcaggct
     1081 atcgccgata cattagtagt ccaggtatta ttttagctgg tgaatcttct ttgtcggtag
     1141 cctatttatt tatcttgttt acgattgtta cttgcattat tgagctggta tttaaaaaat
     1201 tctatttagt gattgccgcg tcaacggact ttgagacagg acaacggatg aaaagtgcta
     1261 tagaagatat ttctcaatcc ttc
//
LOCUS       DACDYI010000177         1215 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.187, whole genome shotgun sequence.
ACCESSION   DACDYI010000177 DACDYI010000000
VERSION     DACDYI010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.187"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            414..947
                     /locus_tag="IWM52_002932"
     CDS             414..947
                     /locus_tag="IWM52_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAP9975104.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DACDYI010000178         1173 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.188, whole genome shotgun sequence.
ACCESSION   DACDYI010000178 DACDYI010000000
VERSION     DACDYI010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.188"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>1173
                     /locus_tag="IWM52_002933"
     rRNA            <1..>1173
                     /locus_tag="IWM52_002933"
                     /product="23S ribosomal RNA"
BASE COUNT          304 a          267 c          362 g          240 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc att
//
LOCUS       DACDYI010000179         1172 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.189, whole genome shotgun sequence.
ACCESSION   DACDYI010000179 DACDYI010000000
VERSION     DACDYI010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.189"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..239)
                     /locus_tag="IWM52_002934"
     CDS             complement(<1..239)
                     /locus_tag="IWM52_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9975105.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            complement(353..694)
                     /locus_tag="IWM52_002935"
     CDS             complement(353..694)
                     /locus_tag="IWM52_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP9975106.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQKLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            complement(681..1010)
                     /locus_tag="IWM52_002936"
     CDS             complement(681..1010)
                     /locus_tag="IWM52_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9975107.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
BASE COUNT          336 a          260 c          138 g          438 t
ORIGIN      
        1 cagtcttggg ttatcttttt ctttcgttta attactggcc gtgctggttt caaatccttg
       61 attccattat gtgttctcca ataatcgaat tcttttcgta atctttcttt ttccatatcc
      121 gttcctttag atagggattt gaatacatct ttaggtgaat aaccacgagc taacagctgt
      181 acataccttc cttttgtcaa agtatccata tccatcgcct ctaaaactgg tcgcgccatc
      241 taattttcct cctaaataaa ttccttgctc aatgcctaag gtaatactac ctttttatta
      301 atcgtcgtga ctccttctaa gcctttatct gtaaaattta ttcctccagt ttttattctt
      361 ctttatccgc tacaaattca atgatttcaa cgctgacaat ataatcactc aaatatgacc
      421 aagtatcttc aacagataat tcgtctccat tatcattcat aagcgtcatt ttgaaccatt
      481 cattaccttc atcatcttca ggtagcaagc cgaatccaat tggattatca gtcaattttt
      541 gatcgtataa atcatcctcg tctaatgcta aaacatctaa tcctttacaa tttccgcctc
      601 ttctaaacgt cacaaatcct ttgtaatccc aacgttggaa agaaacctta atcgtatgtg
      661 tgcccaatcg tatattcgga tcataatttt tcatttgttg taacctcctc ataatcagtt
      721 agtactataa aggtatcttt gtttttatcc aaaaaatcta ataaatcttt tgtagaagat
      781 atctcttcat caacttcacc ataataaata ccttcgataa ttgttgctat atctgaaaaa
      841 ctaattttta ctggacattt atcgtctacc gcattcaata ccgacaaaat tgcttctttt
      901 tctgcaatct ctcgtttgaa tttctttgga gctaatcgca tgacactcaa gtcattttca
      961 agcttttcaa tccatgcagt tttactcatt ttcgcaaatt tttgaatcat tcctctacca
     1021 cctttgctat ctactaaatt agttttactt cataacgaat atctttatcg tttttcgttc
     1081 actcaaaaat atataaaata aatcgactcg tttatggcgt ccgaaacgag aaaccctagt
     1141 agacagcggt aaatgctcga ttgatccaat aa
//
LOCUS       DACDYI010000180         1072 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.190, whole genome shotgun sequence.
ACCESSION   DACDYI010000180 DACDYI010000000
VERSION     DACDYI010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.190"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..483
                     /locus_tag="IWM52_002937"
     CDS             <1..483
                     /locus_tag="IWM52_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9975108.1"
                     /translation="QIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILFFVGYVST
                     KLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKGIRSILKN
                     GQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDLELPMKDK
                     "
     gene            504..716
                     /locus_tag="IWM52_002938"
     CDS             504..716
                     /locus_tag="IWM52_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9975109.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT          410 a          130 c          223 g          309 t
ORIGIN      
        1 caaattgaac aatctggaag aacaacaaaa ggagtgaagg attttttatt gacgtttatt
       61 cactttgtaa atggacacat gtatttgttt gaagtgggta ttattctctt ttttgtcggg
      121 tatgtatcca cgaaacttat taaacgttca attgaaaaag aaattgaaat tgacctgatt
      181 ccgacacttc ttggttatga gtacaatgtt gattttggaa gatatgacga attgatcaat
      241 acggatatcg ttcacacgag tatccatgaa tctactgtta agggcatcag atctatttta
      301 aaaaatggcc aggaaatgaa agcaatagat cgaagaaaag agatctacaa tttttatgaa
      361 gtcgaagcaa gcctacgata tggcatcgga aaattgtggt tagatggaag taatattcgt
      421 caaaacaaac catacatttt ttggaacagt agtgatttag aacttccaat gaaagataaa
      481 taattatgga gaatcgtttg aaaatgattc ctctatacag gattgtgctt atagttggca
      541 tggagttaaa aaccttaaag aaggagcagt tattcaaagt gaaagaacgt cataatcagt
      601 tactatgtag taaaacgggt acgatgaaca ttgaataccg aacagaatat ttagaattaa
      661 cgaatcaaac tgattttttg ttgattcgaa agcaacaaga gcaagaaaaa aattaatggc
      721 tacttatagc caattgattg atgaatggga acaattcaag gaatctaaaa ttccagaacg
      781 tctctaaaag cctttatttg taaagagcat aaggaaacgt gggaacactt tttaaaaaaa
      841 gtgttccaat aggctgtgcc tattacgttt ctcaaaaata aatgtgggaa caaaatattt
      901 aaaaatagga tagtgaaagg atagtgaagg atagtaaaag gagggtagta ggatagcaaa
      961 aaggataggc tccttttcgc tatccttttt aaattggagg aattaatatg gaagtaagaa
     1021 taagaaatgt ggatgagaaa atcgtaagag aaattgatcg aagatgtgac ga
//
LOCUS       DACDYI010000181         1058 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.191, whole genome shotgun sequence.
ACCESSION   DACDYI010000181 DACDYI010000000
VERSION     DACDYI010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.191"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(36..992)
                     /locus_tag="IWM52_002939"
     CDS             complement(36..992)
                     /locus_tag="IWM52_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9975110.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          344 a          176 c          160 g          378 t
ORIGIN      
        1 tagaaaaagc agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct
       61 tcaggaaaat cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata
      121 gtcaacaaaa tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc
      181 actacttgag atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg
      241 aactcaggct ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt
      301 ttcttgataa attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt
      361 tcagaaatca gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga
      421 aaaaatttat cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc
      481 aaatagcaat aaaatgctgg atagttttct ctaaaaaaag aaacatcaag tggaaattca
      541 tcagctttat taatagtgta atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg
      601 atcaattggc tcgtctcttc tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat
      661 cgttcattca accattcgat tgtttcatct tcattttttc tcaagtattt ttgatccaat
      721 ttatcatgaa gataaagcca agagagtgtc ttattgataa aaataacgga tttttctcct
      781 aatactcctt ttaagtggta aaatcctata tccgaaaaaa atagcgactg attgctagca
      841 atatctaaag aatcagcttg ttcagccaac aggttgattg ctaaaaagtg gaataatttc
      901 tttttttcat aagcagacag aggataatac tgttttaaat cttttaacat tttttctgtt
      961 tttataatta taggtagcgt atacaattcc acaaaactta tctctgttga gttaaaaaga
     1021 ttatataaaa aaagccgaat gtttttagga ttccctgt
//
LOCUS       DACDYI010000182         1035 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.192, whole genome shotgun sequence.
ACCESSION   DACDYI010000182 DACDYI010000000
VERSION     DACDYI010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.192"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..>1035)
                     /locus_tag="IWM52_002940"
     rRNA            complement(<1..>1035)
                     /locus_tag="IWM52_002940"
                     /product="16S ribosomal RNA"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DACDYI010000183          998 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.193, whole genome shotgun sequence.
ACCESSION   DACDYI010000183 DACDYI010000000
VERSION     DACDYI010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.193"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..294
                     /locus_tag="IWM52_002941"
     CDS             <1..294
                     /locus_tag="IWM52_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9975111.1"
                     /translation="QKRQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERP
                     DLSFFFVIEGKTIKDLQTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            457..>998
                     /locus_tag="IWM52_002942"
     CDS             457..>998
                     /locus_tag="IWM52_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP9975112.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENL"
BASE COUNT          399 a          151 c          206 g          242 t
ORIGIN      
        1 caaaagcggc aattattaga taaaaatcgt atggaacagt ccttaggaag aaatctacaa
       61 cgattaaaaa aatatgaaaa gaaattagtc gaagaaatcc aggaattgaa acgagcagaa
      121 caagaacgtc ctgacttaag cttctttttc gtcattgaag gaaagacaat caaggattta
      181 caaaccagaa accgtcagtt aatgcgatct agtggcattt tagggttaaa accactctac
      241 aaaaaagaat tgatcaagat tctgtatcga atgaacaata tgaatgatga gtaggaatgg
      301 agaagagaga atgaaatatc cgttgatatg gttagaaaaa gatgttccga aggaagagac
      361 cactagaaaa tttcctcaca ctttaagcct gggtaagttg ggaaaagggc aatcttttaa
      421 ccaagtaaag aatcaaacaa gaagaggtga ctgtacatgt tagaagcaat taaaacagct
      481 ttttcaccac aaaatgatca aaaagtactt gatccattaa agcaactagg aaatcgggaa
      541 atagaaattt taaaatccaa agggtatgat ttagattttt taaaacaagt gatgccacaa
      601 ggtgggttaa attttgatga agaatatgtg acgtatggta atggttattc acgatgtgtg
      661 acagtttatc actacacaaa aaatcctacg cttttttggt tggccaatct catgaacaat
      721 cctcatacag tggccacact cgatgttagc acagatgaaa agcaaaaagt gattgatcaa
      781 atcaaccgcg cattagatga attaaatgat cgatcagata atgaacgaaa agcgactgac
      841 cgaaataaaa gtgcggaaga atacctggaa ttagcggaat atgcccgaaa attgacttct
      901 aatggggaaa tttctaaaca agtcaaagtg agagtacatg tttacggaaa cacgattcaa
      961 gaagtagatg aacgagcgca ggaactgatc gaaaatct
//
LOCUS       DACDYI010000184          998 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.194, whole genome shotgun sequence.
ACCESSION   DACDYI010000184 DACDYI010000000
VERSION     DACDYI010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.194"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..294
                     /locus_tag="IWM52_002943"
     CDS             <1..294
                     /locus_tag="IWM52_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9975113.1"
                     /translation="QKRQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERP
                     DLSFFFIIEGRTIKDLQTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            457..>998
                     /locus_tag="IWM52_002944"
     CDS             457..>998
                     /locus_tag="IWM52_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP9975114.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIKNL"
BASE COUNT          395 a          153 c          210 g          240 t
ORIGIN      
        1 caaaagcggc agttattaga taagaatcgt atggaacaat ccttaggaag aaatctacag
       61 cgattaagaa aatatgaaaa gaaattagta gaggaaatcc aggaattgaa acgagcagaa
      121 caagaacgtc ctgacttaag cttctttttc atcattgaag gaaggacaat caaggattta
      181 caaaccagaa accgtcagtt aatgcgatct agtggcattt tagggttaaa accactctac
      241 aaaaaagaat tgatcaagat tctgtatcga atgaacaaca tgaatgatga gtaggaatgg
      301 agaagagaga atgaaatatc cgttgatatg gttagaaaaa gatgttccga aggaagagac
      361 cactagaaaa tttcctcaca ctttaagcct gggtaagttg ggaaaagggc aatcttttca
      421 ccaagtaaag aatcaaacaa gaagaggtga ctgtacatgt tagaagcgat taaaacagct
      481 ttttcaccac aaaatgatca aaaagtactt gatccattaa agcaactagg aaatcgggaa
      541 atagaaattt taaaatccaa aggatatgat ttagattttt taaaacaagt gatgccacaa
      601 ggtggattaa atttcgatga agagtatgtg acctatggaa atggttattc acggtgtgtg
      661 acagtttatc actacacaaa aaatcctact cttttttggt tggccaatct catgaacaat
      721 cctcatacag tggccacact agatgttagc acagatgaaa agcaaaaagt gattgatcaa
      781 atcaatcgcg cattagatga attaaatgat cgatcagata atgaacgaaa agcgactgac
      841 cgaaataaaa gtgcggaaga atacctggag ctagcggaat atgcccgaaa attgacttct
      901 aatggggaaa tttctaaaca agtcaaagtg agagtacatg tttacggaaa cacgattcaa
      961 gaagtagatg aacgggcgca ggaactgatc aaaaatct
//
LOCUS       DACDYI010000185          994 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.195, whole genome shotgun sequence.
ACCESSION   DACDYI010000185 DACDYI010000000
VERSION     DACDYI010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.195"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            53..>994
                     /locus_tag="IWM52_002945"
     CDS             53..>994
                     /locus_tag="IWM52_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9975115.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDD"
BASE COUNT          356 a          180 c          190 g          268 t
ORIGIN      
        1 tgtgctacac tcaaaatagt tccgacgaaa gaattataag gagtgagtac aaatgaccta
       61 taaccatctt acaccaactg aactcgtaat gatagaagct tatttcaatc aatcacaacc
      121 tgtttcaaaa gtagccaatc tgttacaacg ttctcgacaa actatttata aggtctatcg
      181 ttttcttaaa tctggtggaa gcgcaatgag ctactataaa caatataaga aaaacaaacg
      241 taactgcgga agacatccga ttgttttacc tgatgagcaa caggaataca ttcaaaaaaa
      301 ggttgttcaa gggtgggctc ctgatgtcct tattggtcga gcagagtttc ctattgcttg
      361 ttcggtacgc acactgtatc gaatgtttaa agataaaacc tttgacactc acgatttacc
      421 aatgaaggga aaaagaaaac caaacggtca taaagaaaaa agaggaaaac aagggtttcg
      481 ccggtcaatc cgagacagaa agacagacta tacccagttt gatcatgaat ttggtcacct
      541 ggaaggtgac actatcgtgg gtggaaaaca taaaagcgcg gtcatcacat tagttgaacg
      601 gctatctaaa gtcatcatta ccttgaaacc cgaaggcaga caggctaaag acattgaaaa
      661 taggctgaat caatggtttc gttgtgtccc aaaaaactta tttaaatcca tcacatttga
      721 ttgtggaaaa gaattctcaa attggaaaaa gattagtaat accaacgata tttcaattta
      781 ttttgcggat ccaggaacac cttctcaacg aggattgaat gaacattcaa atggcttatt
      841 acgtaaagat ggtttgtata aagctatgga ttttaatggt gtatctgaaa cctttatcca
      901 atcggtggca tcgaaacgaa atcatattcc tagaaaatca ttacactatc ggacaccaat
      961 ggaagtattt ttgagttacg tcacaaaaga tgat
//
LOCUS       DACDYI010000186          977 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.196, whole genome shotgun sequence.
ACCESSION   DACDYI010000186 DACDYI010000000
VERSION     DACDYI010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.196"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            346..977
                     /locus_tag="IWM52_002946"
                     /pseudo
     CDS             346..977
                     /locus_tag="IWM52_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          332 a          180 c          178 g          287 t
ORIGIN      
        1 tagttatata tttaaatttt taccagaaag aaatgaacac cttcaattat ctaaatctcg
       61 aaaatggtta gatgtaatca tcaggaaaaa caatcttaaa cgaattacta ttcatggtct
      121 tagacacaca catgctagct tgcttttagc aagctagtgc aaatattaaa gatgtgcaag
      181 agcgtttagg ccactcttct attcaaatca ttatggatct atatattcat attacagaca
      241 aaagaaaaga agaaaccgta gcgcaattcg caaaatatag gctctataat ttatgggact
      301 gatgaatcaa aattgattct tcagtcccat tgttatttta ggtatatgat tcgtacattg
      361 aaagagttga aaaactatct tccgaagccg tcaaaaagga ccctgccaaa agtattgatg
      421 gtggatgaat ttcgttcgca cgcttcaatt gtggataaaa tgagctttat ttgtgcggat
      481 ggcgagactg gaaaactcat tgatattttg cctacacgaa aattgtctcg attaacacac
      541 tattttagtc gctgtgcaaa tcctgaagcg gtggaatatt tagtgacaga tatgaacgcc
      601 gcctacttcc aactgacaaa acgtgtctta ccaaatgcga aagtcgtcat tgattgtttt
      661 catatcatca aacatttgaa tcaggcgttc aatgattttc gtgtacaaga aatgaaaaga
      721 ttacaccagt tagggcaacg acgggaagcc gagaaagtga aaaagaactg gcgctttctt
      781 ctaaaaaacc gtcaaaaaat caatcactat gattacaaaa catggcgcag ttttcgtgca
      841 cctaaatacc cgctactcac tgaagcaatg atgattgatc gattgctggc attttcaacg
      901 cctttaaagg acgcctatac ttttttccat gagctgaccg aagcctttag agacaaggaa
      961 tcagacttat tttttac
//
LOCUS       DACDYI010000187          976 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.197, whole genome shotgun sequence.
ACCESSION   DACDYI010000187 DACDYI010000000
VERSION     DACDYI010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.197"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            82..>976
                     /locus_tag="IWM52_002947"
     CDS             82..>976
                     /locus_tag="IWM52_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAP9975116.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHY"
BASE COUNT          331 a          182 c          192 g          271 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatg
//
LOCUS       DACDYI010000188          970 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.198, whole genome shotgun sequence.
ACCESSION   DACDYI010000188 DACDYI010000000
VERSION     DACDYI010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.198"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            17..>970
                     /locus_tag="IWM52_002948"
     CDS             17..>970
                     /locus_tag="IWM52_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAP9975117.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRG
                     VQEVLLFITDGLKGMKDTIHQIYPKAKYQHCCIHVSRNIAHKVRVKDRKEICDDFKAV
                     YQANSKEEANTFLSGMIEKWKKNYPKVTQSLIENQDLLTFYD"
BASE COUNT          352 a          179 c          168 g          271 t
ORIGIN      
        1 aaaagaggac ttccctatga atgattttac tacagaaatt gtgcaaactc tagtcactaa
       61 aggcgattta aatgaattat tccgttcgca cttagaaaaa gcgataaaca cactcctacg
      121 gactgaatta acggcttttt tagattacga aaaatatgat cgcactggtt ttaattcagg
      181 taattcgaga aacggttctt actttcgatc aatcaaaacc gaatatggtg aattaacatt
      241 ggaaatacct agagatcgta atggtgagtt taaacaacaa actttaccag cctacaaaag
      301 aacaaacgat acattggaaa ccactattat ccatttattc gaaaaaggtg ttacgatgtc
      361 tgaaattgct gatttgatcg aaaaaatgta cggtcatcac tatactccac aaaccatgtc
      421 caacatgact aaagttctga ctgaagaagt aaatgccttt aaatccagag ccttaaatga
      481 taagtatgtc gctattttta tggacgctac ttacattcca ctaaaacgtc aaaccgtatc
      541 caaagaagcg atttatattg ccattggtat acgagaagac ggcactaaag aagtactgag
      601 ttatgcgatt gctccaactg aatcaacata cgtttggaat gagctgctac aggatattaa
      661 ctccagagga gttcaagaag tcttgctttt tattacggac ggcttaaaag gcatgaaaga
      721 tactatccat caaatttatc ctaaagcaaa atatcagcat tgttgtatcc atgtatctcg
      781 taacatcgct cataaagtac gtgtcaaaga ccgaaaagaa atctgtgatg actttaaggc
      841 tgtttatcaa gctaactcaa aagaagaagc gaataccttc ttatccggca tgattgagaa
      901 atggaagaaa aactatccta aagtgacgca gtcactcata gaaaaccaag acttattaac
      961 tttttatgat
//
LOCUS       DACDYI010000189          962 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.199, whole genome shotgun sequence.
ACCESSION   DACDYI010000189 DACDYI010000000
VERSION     DACDYI010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.199"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            312..608
                     /locus_tag="IWM52_002949"
     CDS             312..608
                     /locus_tag="IWM52_002949"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9975118.1"
                     /translation="MSAVVTDLTYVRVGTNWAYICLMIDLFNREIIGHSCGNKKDAQL
                     VKRAIQSIPYSLQEIELFHTDRGKEFDNQTIQNLMNGLVIKFLSTVCMKKLDVC"
     gene            complement(531..962)
                     /locus_tag="IWM52_002950"
                     /pseudo
     CDS             complement(531..962)
                     /locus_tag="IWM52_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          300 a          194 c          157 g          311 t
ORIGIN      
        1 ttttacactc tctattattc ttttcataaa tattgtaaat agaagtatta aaaatatata
       61 caaaaagtat tatttcgaga aagataaata ttttcaaaaa ccttttcata atcctcaccc
      121 tttcttacac tgataaatca tgcgtctaat attcaaatta tactcgatat gctttagcta
      181 atcagccaac tctgtttgtc tactatcatt atatcaatag acaatatctg ctcccgtctt
      241 tatgatcgtg ccaaagcaat caaaagaaaa ttgcgaatca cttagatcga aagtttactc
      301 aaacaaaagc attgtcagcg gttgtcacgg atttaactta tgttcgggtc gggacaaatt
      361 gggcttatat ttgtttgatg attgatttat tcaatcgtga aattattggc cattcttgcg
      421 gaaataaaaa agatgcgcag cttgtaaaaa gggccattca aagcattcct tactcacttc
      481 aagaaatcga actcttccat acggatcgtg gaaaagaatt tgataaccaa accattcaga
      541 atctcatgaa tggtttggtt atcaaattcc tttccacggt ctgtatgaaa aagcttgacg
      601 tctgttaaag aatacggtat ccgtccaaag gcttctttta ccaatgaggc atctttcttc
      661 tcaccacagg aataaccaat aatttctcga ttaaacaaat caagtattaa gcagatataa
      721 tgccactttt tccccacgcg aacataagta agatccgtaa cgatggcttc caatggctgt
      781 tcttgtgtaa aggtccgatc taatacattc gctgttttcg cttcattaca agctgttcgt
      841 tgccctttaa aatgagcgat cgtataggta gatgtcaatc cgcggcgttt catgattcga
      901 ccgattcggc gccgactgag ttgaagccca cgctttgcta aacacttctt caattttcgg
      961 gt
//
LOCUS       DACDYI010000190          890 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.200, whole genome shotgun sequence.
ACCESSION   DACDYI010000190 DACDYI010000000
VERSION     DACDYI010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.200"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..888
                     /locus_tag="IWM52_002951"
     CDS             <1..888
                     /locus_tag="IWM52_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAP9975119.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          386 a          116 c          144 g          244 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DACDYI010000191          880 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.201, whole genome shotgun sequence.
ACCESSION   DACDYI010000191 DACDYI010000000
VERSION     DACDYI010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.201"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            13..207
                     /locus_tag="IWM52_002952"
     CDS             13..207
                     /locus_tag="IWM52_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9975120.1"
                     /translation="MIFAAVAAVASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVIL
                     AAASIVVSMFSMFGQTPQGL"
     gene            279..>880
                     /locus_tag="IWM52_002953"
     CDS             279..>880
                     /locus_tag="IWM52_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9975121.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFE"
BASE COUNT          310 a          122 c          202 g          246 t
ORIGIN      
        1 aaaaataatc tgatgatttt tgctgctgtt gcggcagtgg cttccattgc tcttggaggt
       61 attttcttct tctttgggcg aagaggagct gaaaacggga agacacacgt aggttatgta
      121 ttgttggggg taggagttat tttagctgcc gcttctattg tagtttcaat gttctcaatg
      181 tttggacaaa ccccacaagg actataaaaa aaacaagtag aaggagtaaa aagtacgtgg
      241 ctacttttta ctcctttttt tatgaaagga gaatattcat gttttttacg atttcaggaa
      301 atgcaaaatt ggataaagag ttaataagaa aagcaacgaa gaaagtggga aagcgtactt
      361 cctttttacc gaaaacaatt aaatattctg tcattgaaac ggaagaacag ctacatgtgt
      421 taggacaagt gttagaacaa gagaatcaat cttctgttaa attgactctc acgttacatg
      481 ataaagaagg aaaaatagaa aaggctggca cagaatacgt ttttggccca ctggattatc
      541 aaatttcaga aggtttttat cctacattga tggatttaat ccgccttgat gtgtttaaag
      601 atacggatga aactatgagt gtggaagagt cttatcgaaa cgaaaaactt ttacaagttt
      661 tagaagaaga gttagagagc gaccaaagaa aagtgaatca agtgagtgaa gtcgaagtaa
      721 atgaagaagt accatgggaa cgaccaatat ttccaaagaa ggagtcaaaa gaaaaagaga
      781 cagtctatgt tgacgaacaa gctgatattc cgactcagaa atcgatattt gaggaagaga
      841 acgatgtgat gggtcaattg gaggtagatc cctttgaaga
//
LOCUS       DACDYI010000192          837 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.202, whole genome shotgun sequence.
ACCESSION   DACDYI010000192 DACDYI010000000
VERSION     DACDYI010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.202"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..192)
                     /locus_tag="IWM52_002954"
     CDS             complement(<1..192)
                     /locus_tag="IWM52_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9975122.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLK"
     gene            complement(306..647)
                     /locus_tag="IWM52_002955"
     CDS             complement(306..647)
                     /locus_tag="IWM52_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP9975123.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYYQKLTDNPIGFGLLPEDDEGNEWFKMTLTNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
BASE COUNT          241 a          185 c           97 g          314 t
ORIGIN      
        1 tttcaaatcc ttgattccat tatgggttct ccaataatcg aattcttttc gtaatctttc
       61 tttttccata tctgttcctt tagacaggga tttgaataca tctttaggtg aataaccacg
      121 agctaacagc tgtacatacc ttccttttgt caaagtatcc atatccatcg cctctaaaac
      181 tggtcgcgcc atctaatttt cctcctaaat gaattcctta ctcaatgcct aaggtaatac
      241 taccttttta ttattcgtcg tgacttcttc taagccttta tctgtaaaat ttattcctcc
      301 agtttttatt cttctttatc cgctacaaat tcaatgattt caacgctgac aatataatca
      361 ctcaaatatg accaagtatc ttcaacagat aattcgtctc cattatcatt cgtaagcgtc
      421 attttaaacc attcattacc ttcatcatct tcaggtagca agccgaatcc aattggatta
      481 tcagtcaatt tttgatagta taaatcatcc tcgtctaatg ctaaaacatc taatccttta
      541 caatttccgc ctcttctaaa cgtcacaaat cctttgtaat cccaacgttg gaaagaaacc
      601 ttaatagtat gtgtgcccaa tcgtatattc ggatcataat ttttcatttg tcattcctcc
      661 attacgtaaa tttcattttc tctatgtttt atatcaattt tttcacttta ttcaggactc
      721 gttcatattt tatcgttttt cgtttactta aaaatacata aaaaatatcg actcgtttat
      781 ggcgtccgaa acgagaaacc ctagtagaaa tcggtaaatg ctcgattgat ccaatga
//
LOCUS       DACDYI010000193          825 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.203, whole genome shotgun sequence.
ACCESSION   DACDYI010000193 DACDYI010000000
VERSION     DACDYI010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.203"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            1..825
                     /locus_tag="IWM52_002956"
                     /pseudo
     CDS             1..825
                     /locus_tag="IWM52_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          312 a          139 c          153 g          221 t
ORIGIN      
        1 ctgaacaaaa agaattgtta gaactacgta aaagaaacca gcaattagaa atggaaaatg
       61 atattttaaa gcaagcagcg ctgatattcg gacgaagaga caagtaatcg atgcgaataa
      121 gcatctttac cctatatcag cgatgtgcag aatattaggt ctatcacgtc agtcctatta
      181 ttatcaatca aaaccaaaga aagacgaatc agaacttgaa gaagtagtcg ctgaagaatt
      241 tatccgcagc cgaaaggcct acggctcaag aaaaataaaa aaagccttat caaaacgagg
      301 cattcagatc agccgacgaa aaattagtag aatcatgaaa aatagaggat taaaatcgag
      361 ctatactgtt gcttatttta aagtacatca ttctacttgc aatgaagcca aaacgacaaa
      421 cgtattgaat cgtaaattct taagagacaa cccattagaa gcgatcgtaa cagacttgac
      481 ttatgtacga gtcgggaaaa aatggaatta tgtctgtttc attttggatc tgttcaatcg
      541 agaaattctc ggctattctt gtggagaaca taaagatgcc gttctagtaa aaaaagcatt
      601 tagccgtatc aaacaacctc tgacagaggt tgagattttt catactgatc gtggaaaaga
      661 gtttgataac caagctattg atgaattatt aacaactttt gacatcaatc gatcattgag
      721 tcataaaggc tgtccttttg ataatgccgt agctgaatca acttataagt cgttgaaagt
      781 agaatttgtc tatcaataca catttgaaac cttacaacaa ttgga
//
LOCUS       DACDYI010000194          790 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.204, whole genome shotgun sequence.
ACCESSION   DACDYI010000194 DACDYI010000000
VERSION     DACDYI010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.204"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..782
                     /locus_tag="IWM52_002957"
     CDS             <1..782
                     /locus_tag="IWM52_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9975124.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKL
                     IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGI
                     PLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          340 a          107 c          102 g          241 t
ORIGIN      
        1 aagatatgct agattactac tattttgaaa aaaatttatc atataattat gtaatacatt
       61 taaaaacttt tacaggaatg atttttaaat tcgccaaccg tcgatatgga ttaaaatata
      121 atcccgtaaa tggggtttct ttagcaagaa aacctaagac aaacgaagaa ataaaaaaag
      181 aaaaagatgg ttatttatca aaagaagaag ttcaaatgat tgctagtaaa caaaaaagtt
      241 cacatcaaca gtcacgttat tctttaatta ctaaattttt gtttttaact ggactacgtt
      301 atggtgaatt gatatctttg actgaatctg attatgatgg taataaaatt acggtttctg
      361 gtacttatga ttacgagtta aaaattaaaa caacaactaa aaatacaggt tcttatagaa
      421 ccattgaatt atcaagtaat gctaaagaaa tcatagatca attaattgaa gaaaacaaat
      481 taattaagaa taataaagat aaatacattt tcatctctaa aaatggtaac ccgatatcta
      541 ttcaagcata taatcaatct ttaagagcag tatccaaaga attaaacata gataaaaaag
      601 tttcttctca catgttaagg cattcccaca tttctctact tacagaatta ggaatcccat
      661 taaaagccat tatggatcgt gtaggacatg aagattcaaa aacaacatta aagatttata
      721 ctcatacaac taaaaatatg caaaatcaac tagtagaaaa actaggaaaa atagaaattt
      781 aaattgcccc
//
LOCUS       DACDYI010000195          778 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.205, whole genome shotgun sequence.
ACCESSION   DACDYI010000195 DACDYI010000000
VERSION     DACDYI010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.205"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            39..710
                     /locus_tag="IWM52_002958"
     CDS             39..710
                     /locus_tag="IWM52_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP9975125.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQQEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
BASE COUNT          260 a          107 c          181 g          230 t
ORIGIN      
        1 aaaaagattt tttgttttgt ctgaaagggg gtagctggat gagaaaatgg ttatttggtt
       61 ttttaggagt cgctttaatc gtcttttgca gtgtgtttgg ctatgttagc taccaaaaac
      121 atgaaggaga ggtgtttaag caaaatatag aaaaaaagat gcccgtagat caaatcaatg
      181 cacatgcaaa gagttataag gaggatgcaa cgaacgtcaa taatgatatg tctttggggc
      241 aaatgttgtc catacaacaa gaagcaatcg aaatgggcgt caataaacaa gtatttgcac
      301 aaattcagat tcctgcactt ggtttagcat tgccaatttt taaaggggca aatcaataca
      361 ctttgagtct tggagcggct acttattttt atgaagatgc tgaaatgggc aaaggaaact
      421 atgtcctagc aggtcataac atggaaatgc caggtgtttt attttctgat attcaaaagt
      481 tgtcattagg ggaggtgatg gacttagtta gtaacgatgg cgtctatcgt tacaaggtaa
      541 cgcgtaaatt tatcgtacct gagtacttta aattaatcga tggcgttcct gaagagaact
      601 ctttcttgtc tttgccaaag aaaggagaga aacccttatt aacgttgttt acttgtgttt
      661 atacgtcaca aggaaaagaa agatacgtgg tacagggaga actacaataa caaggtttta
      721 atcaacgtga gtcgttgaaa tatgaaatga ggagagtaaa aaatggcaat aaatcggt
//
LOCUS       DACDYI010000196          755 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.206, whole genome shotgun sequence.
ACCESSION   DACDYI010000196 DACDYI010000000
VERSION     DACDYI010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.206"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(463..>755)
                     /locus_tag="IWM52_002959"
     CDS             complement(463..>755)
                     /locus_tag="IWM52_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9975126.1"
                     /translation="LIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLVE
                     LDVNNYRELYVFNSLDFEIPMKQMKNIDIHILPSESCQIEYSIEYFYKGKKM"
BASE COUNT          260 a          101 c           85 g          309 t
ORIGIN      
        1 aacaataaat gaagactttt ttattgttaa ttgtttattt ttctattatt agatatgaaa
       61 atcgcattta gataaaatag aaaaatatga atatatttta aatattaaga tgagagtcat
      121 taacttatga tagtagtcaa tttgcttttc actaattaca aaatatgtga tataggaagt
      181 ttagttattg ttctcataat gccatcctgt ttttagatta tctagtaatt tttttgttat
      241 ttaaaattag gagcagtgcc aattgtttta attttaaagg ctttttttat atcttctaaa
      301 ttagaagata tttgtatatc aatatggaat atattacctt ttttgtattt ttttatgtta
      361 tctttagcta aaacatcaaa tacatgtcct tgaaattgta tcgaggcagc tggatttagt
      421 ttaaattgag gaaactttac cacagctgat tcaaaacact tgtcacattt ttttaccttt
      481 gtaaaaatat tcgattgaat attctatttg gcacgactca cttggcagta tatgtatgtc
      541 aatatttttc atctgtttca taggtatttc aaaatctaaa ctattaaaaa catataattc
      601 cctgtaatta tttacatcta actctactaa atttatcttt ggaacaatag atcctccacc
      661 cattccacca taataaaaaa aatcttgttc tctatcaaaa tagcacatat taagctcagt
      721 ttgtttatca tcctcaataa ttttataaat caaac
//
LOCUS       DACDYI010000197          742 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.207, whole genome shotgun sequence.
ACCESSION   DACDYI010000197 DACDYI010000000
VERSION     DACDYI010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.207"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            51..731
                     /locus_tag="IWM52_002960"
     CDS             51..731
                     /locus_tag="IWM52_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HAP9975127.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEI
                     KVLLGIPA"
BASE COUNT          266 a          125 c          148 g          203 t
ORIGIN      
        1 tactatcaaa taaggtagaa taatagaaaa agatagcagg aggaatgacg atgaatcatt
       61 ttaaaggaaa gcaatttcag caggatgtga ttattgtagc cgtgggctac tatcttcgtt
      121 ataaccttag ctatcgtgaa gttcaagaaa tcttatatga tcgtggcatt aacgtttctc
      181 atacgacgat ttatcgttgg gtgcaagaat atggcaaact actctatcaa atttggaaaa
      241 agaaaaataa aaaatccttt tattcatgga aaatggatga aacgtacatc aaaattaaag
      301 gaaaatggca ttatttgtat cgagccatcg atgcagatgg tttaaccttg gatatttggt
      361 tacgtaaaaa acgggacaca caagcagcct atgcttttct taagcggtta gtgaagcagt
      421 ttgatgaacc gaaggttgta gtcacagata aagccccctc tattacaagt gcctttaaga
      481 aactaaaaga atacggcttt tatcaaggga cagaacatcg taccattaaa tacctgaata
      541 atttgattga acaagaccat cgtccagtaa agagacgcaa taaattctat cgaagtttac
      601 gcactgcctc taccacgatt aaaggcatgg aagccattcg aggattatat aagaaaaccc
      661 gaaaagaagg cactctcttc gggttttcgg tctgtactga aatcaaggta ttattgggaa
      721 tcccagctta aatcatagat ac
//
LOCUS       DACDYI010000198          720 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.208, whole genome shotgun sequence.
ACCESSION   DACDYI010000198 DACDYI010000000
VERSION     DACDYI010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.208"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(111..>720)
                     /locus_tag="IWM52_002961"
     CDS             complement(111..>720)
                     /locus_tag="IWM52_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9975128.1"
                     /translation="DHQDELHSNENSEEGQNGQLPKTDPEQYRYKSNINSENEKRISD
                     LPKRNQEVQTDDDSIDLPNDNGVEVGTEKSTKIGLPKAQNLPMKDSRLNQFKENDQSF
                     VHQSTAGNVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPEPIQRVHT
                     GYKPANVAFTAPTKINVTRETKNRNNELKEQLLRDIKQDIKP"
BASE COUNT          175 a          135 c          116 g          294 t
ORIGIN      
        1 aaaaacttcc attccccact aaatcttgga ggatttttca tgttaatata tttatccata
       61 aatgatttct ccttttcata catagaaaaa aggcctaact tgtaggcctt ttatggtttg
      121 atatcctgtt tgatatccct caatagttgt tctttcaatt cattgttcct gtttttagtt
      181 tctcttgtca catttatttt tgttggtgca gtaaatgcca catttgctgg tttgtaacct
      241 gtgtgaactc tttgaatagg ttctggtgca gcttctcttg tcacaacatt ttgaacggac
      301 gcattccctg tctccccact ttcagataaa ttgtaagaaa cagttgtcag atttccattt
      361 tcaggcaaac gacttggttt catttgaaca ttacctgcag ttgattggtg aacaaagctt
      421 tgatcattct ccttgaattg attcaaacga ctatctttca ttggtaaatt ttgtgcctta
      481 ggtaaaccaa ttttcgttga tttttcagtt ccaacttcga ccccattatc atttggcaga
      541 tcgatggaat catcatccgt ttgtacttct tgatttcgtt ttggtaaatc tgaaatgcgc
      601 ttttcatttt cagaattaat gttagattta taacgatatt gttctggatc tgtttttggt
      661 aattgaccat tttgtccttc ttctgaattt tcgttagaat gcaactcatc ttggtgatct
//
LOCUS       DACDYI010000199          700 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.209, whole genome shotgun sequence.
ACCESSION   DACDYI010000199 DACDYI010000000
VERSION     DACDYI010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.209"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            13..207
                     /locus_tag="IWM52_002962"
     CDS             13..207
                     /locus_tag="IWM52_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9975129.1"
                     /translation="MIFAAAAAVASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVIL
                     AAASIVVSLFGLFGQTPQGL"
     gene            277..>700
                     /locus_tag="IWM52_002963"
     CDS             277..>700
                     /locus_tag="IWM52_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9975130.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHESRSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQ"
BASE COUNT          239 a           99 c          160 g          202 t
ORIGIN      
        1 aaaaacaatc tgatgatttt tgctgctgct gcggccgttg cttccattgt tattgggggt
       61 cttttcttcc tatttggacg aagaggagct gaaaatggga agatgcacgt aggctatgta
      121 ttgatggggg taggagttat tttagcagca gcttctattg tggtttcgtt atttggattg
      181 tttggacaaa cacctcaagg attataagaa agtaaaaaag taggagtaag aagtagtcga
      241 tactttttac tcctttttta tgaaaggaga atactcatgt actttacgat tgcaggcaat
      301 accaaaacag ataaagagtt aataaaaaaa gcaacgaagg aagtaggaaa gcgtacttct
      361 tttttaccga aaacaattaa atattctgtc attgaaacgg aagaacagct acatgtgtta
      421 gggaaagtac tggaacacga aagtcgatct tctgttaagt tgactctcac gttacatgac
      481 acagaaggaa aaatagagaa agctggtaca gaatacgttt ttggcccact ggattatcaa
      541 atttcagaag gtttctatcc tacattgatg gatttaatcc gccttgatgt gtttaaagaa
      601 acggatgaaa ctatgagtgt ggaagagtct tatcgaaacg aaaaactttt acaagtttta
      661 gaagaagagt tagagagcga ccaaagaaaa gtgaatcaag
//
LOCUS       DACDYI010000200          699 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.210, whole genome shotgun sequence.
ACCESSION   DACDYI010000200 DACDYI010000000
VERSION     DACDYI010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.210"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..459)
                     /locus_tag="IWM52_002964"
     CDS             complement(<1..459)
                     /locus_tag="IWM52_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HAP9975131.1"
                     /translation="MSIITSNKLTEYDEFAMKHWGEGGNDNGTITGEWTNPFPGSSLD
                     KSTFSGGQLFGTNPGSEFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGL
                     GACVIVINYDGLNMVYQEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLG"
BASE COUNT          207 a          181 c          110 g          201 t
ORIGIN      
        1 acctaaatgc aagtgtgctg tatctcggat cccgattact tgaccaactt tcacctgatc
       61 tccaaccttc acacgagaat ttcctgtact attcgcaaat tcttgatata ccatattcaa
      121 cccgtcgtaa ttaatcacga tcacacaagc acctaaacct gaaataccag gattgccaac
      181 gtacacgact tttcctccat gcaccgcgtg aatttcactt cctggatgat caactgaacc
      241 aaaatctaat ccatcatgga agccattcgg acggaattca ctgcctggat ttgttccaaa
      301 taattgacca ccgctaaagg tacttttatc taaactgctg ccagggaatg gattcgtcca
      361 ttcaccagta attgtgccat tatcattacc accttcaccc caatgtttca tcgcgaattc
      421 atcatattcg gttaatttat tagaagtgat aattgacata caacctgtat aatacggact
      481 atcttcagta attcctgcag cggcataacc acctcgtcct aagatcaata agcttgcgcc
      541 agggctttta tttcctacac atgcattaaa atttgattgt gtggctacca taaagccgta
      601 ataggagaat gcagcttttg agttgggaaa attcatgtac cagccacctt caataccacc
      661 aattcctcgt gcagttcctt taggaaacaa acagccatc
//
LOCUS       DACDYI010000201          689 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.211, whole genome shotgun sequence.
ACCESSION   DACDYI010000201 DACDYI010000000
VERSION     DACDYI010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.211"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            82..>689
                     /locus_tag="IWM52_002965"
     CDS             82..>689
                     /locus_tag="IWM52_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HAP9975132.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRK"
BASE COUNT          230 a          119 c          135 g          205 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccattggac ggctcaaagt tattttgttc aacccagaca ttcaatcgcg
      421 aatcatgtca gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaaatt gtcaggtatc tttgaccacc gttattcgta cgttgaaaga gtttaaaagc
      541 tacttaccaa agcaatctaa gaggattctc cccagagtat tgatggttga tgaatttcgt
      601 tcgcacgctt ccatagaaga taaaatgagc tttatttgcg cagatggcga aacaggacaa
      661 ttaattgatg tcttgcctac gcgtaaatt
//
LOCUS       DACDYI010000202          685 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.212, whole genome shotgun sequence.
ACCESSION   DACDYI010000202 DACDYI010000000
VERSION     DACDYI010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.212"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..188)
                     /locus_tag="IWM52_002966"
     CDS             complement(<1..188)
                     /locus_tag="IWM52_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9975133.1"
                     /translation="MVHSTIGVVVEKSRDNLVFVTEIQTGRSFVVTDKAAKAYQSSDI
                     LTLNLTTKTFVDATEDYP"
     gene            complement(334..669)
                     /locus_tag="IWM52_002967"
     CDS             complement(334..669)
                     /locus_tag="IWM52_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9975134.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVNGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
BASE COUNT          195 a          148 c           96 g          246 t
ORIGIN      
        1 aatggataat cttctgtcgc atcgacgaac gtcttcgtag tcagatttaa cgttaaaata
       61 tcgctacttt gataagcttt tgccgctttg tctgtgacga caaaagatct gcctgtttga
      121 atttctgtca caaaaacgag attgtctctg cttttttcaa caacaactcc aatcgttgaa
      181 tgaaccattt ctgcttcctc ccttctcttt cttttgatat aatgtagtcg caaatcgcac
      241 cactcaatga gttggcggaa aacaattcat tgaaaaaaag agtcctggac aatcacgttc
      301 aggactcttt tacttctttt attcacttaa caactaacac tctgtgagtg aaatggattc
      361 ttcaatgctt ttacatggaa agttattgat tccctcttca ttcatttccc aagtagtata
      421 ataaggtgct aacttgccct catattcttg aatgataaaa tatttttcac cattcacatg
      481 attacaacaa tacacaccag tagctgggta ctcaacgact aaaaattgat ttggatcaaa
      541 ctgttcgttt ctcaacattt ctcttatctg gaacaaatgc tctttttcta aacaatcagg
      601 aagttgttcg attcgttctt ctcttaatga tccttctata actttccatt gatctaaata
      661 ttgtttcatt gctattcctc ctact
//
LOCUS       DACDYI010000203          668 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.213, whole genome shotgun sequence.
ACCESSION   DACDYI010000203 DACDYI010000000
VERSION     DACDYI010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.213"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..623
                     /locus_tag="IWM52_002968"
     CDS             <1..623
                     /locus_tag="IWM52_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9975135.1"
                     /translation="DFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIEI
                     VDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILDE
                     NVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIFD
                     VKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
BASE COUNT          292 a           47 c           95 g          234 t
ORIGIN      
        1 atgacttttt taaaggtgca tcccatgaac ttaaaacccc tcttgctagt cttaaaataa
       61 tactggaaaa catgaaatac aatattggaa aatataaaga aagagatatt tatattaatg
      121 aatgtattga aatcgttgat agtttaacaa aaaatatttc tcaaatatta tcagttcatt
      181 ctatagaaaa tttgaataat gatgaagaat atttgaaaat aaatgataca ttagaagaca
      241 tattaaaaaa gtatgaaata ttagcaaatc aaaagaaaat aactgtcaat aattatatat
      301 tagacgagaa tgtttatata ggaaaaacag ctttaaagat tattctatct aatttgatta
      361 gtaatgcggt aaaatatact gatgtaaatg gggtaattaa tattgggata gttaatgatt
      421 ggttatatat agagaattca tacggtaaca ataaaatttc gaatatggat aaaatatttg
      481 atgttaaatt tgatttaaat aaggaaaata gtaatggatt aggtttatat attgtaagta
      541 atattctgaa taattacaat atagaatata aagtattgca ggatgaagaa ttttttatct
      601 ttaaaataaa aatattttct taattaagat ataattttgc aatagttatt gaaaaataga
      661 gacttatt
//
LOCUS       DACDYI010000204          660 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.214, whole genome shotgun sequence.
ACCESSION   DACDYI010000204 DACDYI010000000
VERSION     DACDYI010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.214"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>660
                     /locus_tag="IWM52_002969"
     CDS             <1..>660
                     /locus_tag="IWM52_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9975136.1"
                     /translation="KEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVAKINNFTQT
                     NQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTTTEVLMEKY
                     EKIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLELKKREQDLAEQQMAI
                     EKQRLNQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPTPSVAAVQTPMNNQTNLTP
                     SP"
BASE COUNT          314 a          109 c          121 g          116 t
ORIGIN      
        1 aaaagaggaa ctaaataaag aagaacagcg agtgattgaa gaagaagtac aaaaaattaa
       61 agagcgatac gaaaagaaac gcgacaatgc gtattccttg tgtgtagcaa agatcaataa
      121 ttttactcag acgaatcaag aagcgttatc gaaagaaaaa gaaaacttgt taaaagaacg
      181 tattaaagaa cgaaaagaag aattttaccg aggtttgcgt acagataagc taaagattag
      241 caaagaatta aatgaacaac tgaataagct atatcaaaca acgactgaag ttcttatgga
      301 aaaatacgaa aagatccagc aagaattaca agtgcaaatt ccaaaatgga aaaaagagta
      361 tgaaattcaa caacaagaat tgaaagaaca agaagaaaaa caacggcaaa aagaaaaaga
      421 gcgcgaacaa ttagaactaa aaaaacgtga acaggatctt gcggaacaac aaatggcaat
      481 agaaaaacaa cggttaaacc aagaaaagga aaaagaagca gctgctcgta aggaacgtca
      541 agaacaaatg gatacattgc gacaagctca gttaaattat ctagcacaac ctcaaccaac
      601 gccttcagta gctgctgttc aaaccccaat gaacaaccaa acaaatctaa ctccttcacc
//
LOCUS       DACDYI010000205          627 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.215, whole genome shotgun sequence.
ACCESSION   DACDYI010000205 DACDYI010000000
VERSION     DACDYI010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.215"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
BASE COUNT          280 a           91 c          101 g          155 t
ORIGIN      
        1 aattccttag gcagcttttg ctgtgtagaa aggaataaat aataacgaaa gttcaaaagc
       61 taatgccgac tattctagac attaaagaag acgaaaacga acgataaaga aagaaaagtt
      121 atgaataatc gtttattgaa aaaaacttga aaaaatacaa aaaattcgtt gaccaagcta
      181 aatttagttg gtatattatt acttgtccaa aagacagaag aaaatattta ttcaaaaaca
      241 taacaaaaaa gttattgaca tgttactaac gacatgataa gataactgag tcgttaaaaa
      301 agaaccactt gcgaagcttg attctatcga aaaaaacttc aaaaaagttc ttgacaaata
      361 acaaacgata agttatgata aatgagttgc cgaaagattt cgaaaaacga aatcgaaaaa
      421 aacttttaaa aaagagttga caatcgcttg tctctctgat atgatataaa agttgctacg
      481 gcagccaaaa atgaatcggg aaacactctc gatgaagtag acctttgaaa actgaacaaa
      541 gtaagacaaa ccaaatgtgt agggcgtctt gattcaattc aagacaacaa acatttttaa
      601 caagcaagca atatgctagc aaacaaa
//
LOCUS       DACDYI010000206          625 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.216, whole genome shotgun sequence.
ACCESSION   DACDYI010000206 DACDYI010000000
VERSION     DACDYI010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.216"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
BASE COUNT          239 a           80 c           78 g          228 t
ORIGIN      
        1 aaggtatcaa gagtcacagt gaatttattc cgaaaagtga aaagagcaaa tataaaattt
       61 aataaaaata tagaactaaa taggaaaacg tagacgtaat tttctacgtt ttccttttca
      121 tatatattaa atattacgga aaattatata aactaattta gtgttttgtt tgtaattcct
      181 aattttgctc cacttttaga taatttttag gtctatttaa ctcatcagac attctatgct
      241 cattttctcc aaatttgttt caactctttc gtacttaatt ttgcgtattt gaaaattctt
      301 cttttagagt acttttatta cgatactagt atgatatata tctatagcaa acagttaaaa
      361 tcttcctaaa ataagcgaat agcattccta aaacctataa aatttttaga tgaagcgaca
      421 agtggacaat taataaacta cttgaacatg aaattatgga tcacctttca aatttagaaa
      481 atggaattct tatacttatt gcacaacatt tattgattgc aaaattatgt gacaaaaaga
      541 actagtagaa gaaggaataa tggcttgata ataaagtact acctattaaa ggtagtactt
      601 tatttaattt agtataaatt aacat
//
LOCUS       DACDYI010000207          608 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.217, whole genome shotgun sequence.
ACCESSION   DACDYI010000207 DACDYI010000000
VERSION     DACDYI010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.217"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>608
                     /locus_tag="IWM52_002970"
     CDS             <1..>608
                     /locus_tag="IWM52_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HAP9975137.1"
                     /translation="MNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNEL
                     RKVGQKSQAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFS
                     TSLKEAYPYFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNAM
                     IYPYTNGKIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          212 a          123 c          112 g          161 t
ORIGIN      
        1 acatgaacgc cgcctacttc cagcttacca aacgtattct gccaaatgcg aaagtagtga
       61 ttgatcggtt tcatattatc aaacacatga atcaagcctt taatgagttg cgtatccgtg
      121 aaatgaatga acttcgtaaa gtaggacaga aaagtcaggc agaaaaactg aaaaagaact
      181 ggcgcttttt gctaaaaaat cgtgcaaaca tcaaccatta tgaatacaaa acatggaaaa
      241 gttttcgagc accaaaatac ccatttctta cggaagcgat gatgattgat cgattgcttg
      301 gcttttctac gtccctgaag gaggcgtatc cctattttca tgaattagtt gaagcctttc
      361 gagacaaaga ccctgactta tttttctcct tattggcaga acttcccgaa acgttagatg
      421 acggctttcg ggaaaagctc caaaaccttc tgacctatga agaaggcatc accaacgcga
      481 tgatctatcc ttacaccaat ggaaaaatag aagcgaagaa tacccatata aagacaatga
      541 aacgagtatc ctacggattt aaatcatttg agaacatgag aattagaatc tttttgatca
      601 atcaatta
//
LOCUS       DACDYI010000208          592 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.218, whole genome shotgun sequence.
ACCESSION   DACDYI010000208 DACDYI010000000
VERSION     DACDYI010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.218"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..579
                     /locus_tag="IWM52_002971"
     CDS             <1..579
                     /locus_tag="IWM52_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9975138.1"
                     /translation="GIKSHLWVTQNIWGDHTDPCGYLSEMGVSKEKLAYDLAHGFTDD
                     NPTTSDDNPTTSEDKPVIDPTRAGAANSTLTDGTNYAHVDQFGEIENANLHVAGWHIA
                     NYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVY
                     VMMRATNDPEGNTKGGAQDFHDKRWYLNIPQR"
BASE COUNT          203 a          105 c          122 g          162 t
ORIGIN      
        1 ggaatcaaat cgcatttatg ggtaacacaa aatatttggg gcgatcatac agatccttgc
       61 ggttatcttt ctgaaatggg tgtaagtaaa gaaaaactag cctatgattt ggctcatggt
      121 tttacggatg ataatccaac tacttcggat gataatccaa ctacttcgga ggataaacca
      181 gtaattgatc caactcgagc aggtgctgca aattctacgc tgacagatgg aacaaattac
      241 gcccacgttg atcagttcgg agaaatcgaa aacgcaaact tgcatgtagc tggatggcac
      301 attgctaatt ataaatacga gtatattttc attatggact acaatactgg aaaagaacta
      361 gctagagtaa gagctgatgg gatttataga ccagacgtaa atcaagctta taatacttta
      421 ggaaatgttg gctatcatgt atctttcaat atgcgtgatt tccctaataa gaaagtatac
      481 gtcatgatgc gtgcgacaaa cgatccagaa ggaaatacta agggtggagc acaagatttt
      541 catgacaagc gttggtattt aaatattcca caacgataaa aaattccccc ct
//
LOCUS       DACDYI010000209          580 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.219, whole genome shotgun sequence.
ACCESSION   DACDYI010000209 DACDYI010000000
VERSION     DACDYI010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.219"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>580
                     /locus_tag="IWM52_002972"
     CDS             <1..>580
                     /locus_tag="IWM52_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9975139.1"
                     /translation="AEEKEYIEKRSTEGWTPDVIIGRAERTFSCSVSTLYRRFKTGEF
                     NVLHLPMQGKRKPNGYKEKRGKQAFKRNISERKKDYVVFEEEFGHLEGDTIVGIHHKS
                     AVITLVERLSKAIIALKPEGRKAVDIENSINEWLQSVPKNLFKSITFDCGKEFSNWKS
                     ISNTNDIDIYFADPGTPSQRGLNEHSNGLLRK"
BASE COUNT          205 a          108 c          117 g          150 t
ORIGIN      
        1 ctgctgagga aaaagaatac attgaaaaaa gatcaactga aggttggacc ccagacgtca
       61 ttattggccg tgcagagcga accttctctt gttcagtaag taccctctat cgccggttta
      121 agacgggaga attcaatgtt ttacatttac cgatgcaagg aaaacgaaaa ccaaatggtt
      181 ataaggaaaa acgtggaaaa caggcattca aaagaaatat ttctgaacgt aaaaaagatt
      241 atgtcgtttt cgaagaagaa tttggacatt tagagggtga tacgattgtc ggcatccacc
      301 ataaaagtgc cgtcatcaca ctcgttgagc gactttcaaa agccattatc gccttgaaac
      361 cagaaggccg taaggcagtt gatattgaaa attcgattaa tgaatggctt caatccgtac
      421 ccaaaaatct tttcaaatca attacctttg attgtggaaa agaattctct aattggaaaa
      481 gtattagtaa tacgaatgat attgatattt atttcgcaga cccaggaacg ccttcccaac
      541 ggggattaaa tgaacattca aacggactcc tccgaaaaga
//
LOCUS       DACDYI010000210          546 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.220, whole genome shotgun sequence.
ACCESSION   DACDYI010000210 DACDYI010000000
VERSION     DACDYI010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.220"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..>546)
                     /locus_tag="IWM52_002973"
     CDS             complement(<1..>546)
                     /locus_tag="IWM52_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP9975140.1"
                     /translation="ATNKITSAVDENGEITFADQPAYVNSKGNVYVVYESKSAAGLVT
                     QKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTKFGDDGTAQSKQTPLKGA
                     KFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEVPSEVVVGSDKEPTADQY
                     LLGADARNDAHNKLTFEITND"
BASE COUNT          148 a          114 c           89 g          195 t
ORIGIN      
        1 catcattcgt aatttcaaag gttaatttat tgtgcgcatc gttgcgagcg tctgcgccta
       61 ataaatattg atcggctgtt ggttctttat ctgatcccac tacaacttct gaaggaactt
      121 ccacaaaata gtattttcct aacgtcaaat cagtagcagt cagctttcct tgatcatccg
      181 atacaagatc tcctaatttt gttccttttc caggttcgcc cttgtataac tcaaattttg
      241 ccccttttaa aggtgtttgt ttgctttgag ctgtgccatc gtcaccaaat ttcgttaatt
      301 caaaactcaa cttagaaaca atatttttag gatataagtg aatgtctttt aaaaaacctg
      361 aacctgtatt atcagtcatt ggcgcaatca caaccattgg ttttgctttt tgtgtgacta
      421 atccagcagc ggatttactt tcataaacca catatacatt ccctttggaa ttgacatagg
      481 ctggttgatc agcaaaagtg atctcaccat tttcatctac agctgaagta attttgttgg
      541 ttgcat
//
LOCUS       DACDYI010000211          539 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.221, whole genome shotgun sequence.
ACCESSION   DACDYI010000211 DACDYI010000000
VERSION     DACDYI010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.221"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(264..527)
                     /locus_tag="IWM52_002974"
     CDS             complement(264..527)
                     /locus_tag="IWM52_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circular bacteriocin, circularin A/uberolysin
                     family"
                     /protein_id="HAP9975141.1"
                     /translation="MKKNLLLVLPILGFAGFFVGVPMLSANIGISSYAAKKVIDIINT
                     GSTVATIISIVAAVVGGGLITAGIVATAKSLIKKYGAKYAAAW"
BASE COUNT          212 a           93 c           75 g          159 t
ORIGIN      
        1 tgaatttttt catgttgaaa acatcactaa tcaaaggcaa attataattt agaaatagaa
       61 tgaaaacact aaatgaaaat agaacattag aaaaaataga atcaaaaaaa cccaagacta
      121 gtgccgaaat aagggaaaca atcgaagcta caaaaaataa tgatttcgtt gaacaggacc
      181 aatttatttg tttccgatat ttctcaaaaa gaactttctt tgggaaagtt aaaggcgact
      241 gtaaagtcgc ctttagtcct tttttaccaa gctgctgcat atttagctcc atatttttta
      301 attaatgatt tggctgtagc aacaatacca gctgtaatta atccgcctcc aacaactgca
      361 gcaacaatag aaatgattgt agcaaccgtg ctaccagtat taattatgtc aataactttt
      421 ttagctgcat aagaagaaat tccaatgtta gcacttagca ttggtacacc aacaaagaat
      481 cccgcaaaac ctaaaattgg taaaactaat aataaattct ttttcataat aattcctct
//
LOCUS       DACDYI010000212          527 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.222, whole genome shotgun sequence.
ACCESSION   DACDYI010000212 DACDYI010000000
VERSION     DACDYI010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.222"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(60..281)
                     /locus_tag="IWM52_002975"
     CDS             complement(60..281)
                     /locus_tag="IWM52_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9975142.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
BASE COUNT          184 a           88 c          102 g          153 t
ORIGIN      
        1 tgttttcatt aagacgtatt acctaagagt tccatgactt tagtctggta gcgtgtcagc
       61 taaccaaaaa agagaaaaag aaaagtaaaa aattcgactt tcttttttct atagtatata
      121 aatttttgca tatagataaa gatcaagata ccgataacgg aagctagatt gtaatctatt
      181 ttagtgacag tcaggtatag ccaagtaact agaagatcag tggaacctgc accataaaag
      241 tttgagtttg tcggtccgtt gacagttaaa agaaaaatca cgttgaagtt attgatattt
      301 ccgattacgc cgcttcgatt tggtcacgat ccaaattttg gataatccct gtagaaatta
      361 gcatagtagc tgggatacca attcacatat tgacaaagat aacagagact tttacataaa
      421 acggatcaat caggaaagga attgctcgat ccagccccag ttttccaaca atgtgttaac
      481 tggcctagcg tcattcagca aattatgcat gatgaggagc gaaatga
//
LOCUS       DACDYI010000213          521 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.223, whole genome shotgun sequence.
ACCESSION   DACDYI010000213 DACDYI010000000
VERSION     DACDYI010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.223"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>521
                     /locus_tag="IWM52_002976"
     CDS             <1..>521
                     /locus_tag="IWM52_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP9975143.1"
                     /translation="TSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLHD
                     KDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKIRSPNPQKCANAKGMVRYFAHMD
                     NPEKFQYAKSDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDLIDYAM
                     SERFDDWFPLLCD"
BASE COUNT          170 a           71 c          120 g          160 t
ORIGIN      
        1 tgacgagtag aaaaactaaa gatgaacgca caaggaattg gacgtttgta gtctatcctg
       61 aatcggctcc tgaaaattgg agggagtttt tagatgagtt acatgttcct tgggttgaga
      121 gtccacttca tgataaagat gtgaatcctg acggtgaatt gaaaaagcct cattggcatg
      181 ttttgataat gtttagcggt aaaaagacgt atgatcaaat acgtgagata actcaaaaga
      241 ttcggtctcc aaacccgcaa aaatgtgcta atgcaaaagg tatggtgcga tattttgctc
      301 atatggataa tccagaaaaa tttcaatatg ctaaaagtga tattatcgct catggtggtg
      361 cagaaattgc tagttatttg tcagtaacga gtgcagaacg atatgagttg attagagaga
      421 tgatgtcgtt tgttgattct aagaatatca ctgaaatcaa agatttaatt gactatgcaa
      481 tgtcagaaag atttgatgat tggtttccgc ttctttgtga t
//
LOCUS       DACDYI010000214          517 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.224, whole genome shotgun sequence.
ACCESSION   DACDYI010000214 DACDYI010000000
VERSION     DACDYI010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.224"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
BASE COUNT          225 a           53 c           82 g          157 t
ORIGIN      
        1 tatatccaac aaagaataag gtacaaaata caagcaagtg agaaattcag ggatgtcatt
       61 ataaaaaaac tagcgaacaa ttaaatagaa taacatccgt taattaaatg taagtatctt
      121 ttagatgaac gaaaaaacaa taaaatgctg taatattcgt gtttaaaaat aataaataaa
      181 aatagggaat tttctattgc aattttataa agagattgct atattattat ttgttctaaa
      241 tgattccttt aaggtgtcac taaaaattat aaaaaatagt gttgacgcac ttttgatgtc
      301 atgttataat aacgaagtcg tttggatgag atagctaata aagcttgatt ttatcgaaaa
      361 aaacttcgaa aaagttattg acaaataaca ataaagatgg tatgataatc aagtcgctgc
      421 taatgagtgg caaagacgtt tagaaaaaat aaacaaagaa gttgttgaca ttctcttctg
      481 aatctgataa gattaaagag ttgttaaaac aaaaaga
//
LOCUS       DACDYI010000215          490 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.225, whole genome shotgun sequence.
ACCESSION   DACDYI010000215 DACDYI010000000
VERSION     DACDYI010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.225"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..387
                     /locus_tag="IWM52_002977"
     CDS             <1..387
                     /locus_tag="IWM52_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP9975144.1"
                     /translation="RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGK
                     CAITLEEFQQANEIHCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIAL
                     LKLNKVQLIRLNKYRVLAGNQELNLI"
BASE COUNT          190 a           77 c           89 g          134 t
ORIGIN      
        1 agaaaattta tccacaccaa tctaagtgtt gataaattac tgatgtacca gttaatgaaa
       61 ttatctttgg aaaatcgcac aatagaatac gcagacaatc gaatttcgct attttctgct
      121 caacatggaa aatgtgctat aactctggaa gaatttcaac aagctaatga aatacattgt
      181 catcatattg taccaactgg agctggtgga aatgacgatt ataaaaatct tattttggta
      241 aaagaggcgg tacaccgtct aatacacgct aaaacagaag aaacaataca aaaatatata
      301 gctctattga agctaaataa agtccagctc ataagactaa ataaatatag agtattagct
      361 ggcaatcaag aattaaactt gatataggtc tactacataa atacttttca atataaacac
      421 gatggaacgc ggagtgcggt gaaagtcgca cgctccgtgt gaatgggggg aaaagtttga
      481 gatatttata
//
LOCUS       DACDYI010000216          462 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.226, whole genome shotgun sequence.
ACCESSION   DACDYI010000216 DACDYI010000000
VERSION     DACDYI010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.226"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..430)
                     /locus_tag="IWM52_002978"
     CDS             complement(<1..430)
                     /locus_tag="IWM52_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9975145.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DACDYI010000217          462 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.227, whole genome shotgun sequence.
ACCESSION   DACDYI010000217 DACDYI010000000
VERSION     DACDYI010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.227"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
BASE COUNT          131 a           77 c           95 g          159 t
ORIGIN      
        1 ttgacaacca tttttgttag gcgtttgagg aagtgaagct gttttcctca tgttttaaaa
       61 gctagaaata aattacagtg tacgctcctt tcttttttcg ttttgttggt tcattttcgc
      121 cagtgtgttc gtgtcgtttg ctgtgacgag tacgcaacat agacacaaag aaaacacaag
      181 ggcgagcttg ctcgtttata tacccttgtg ttttcgactg gatatgttaa ggtaacaaac
      241 agtaaatgac acgttacaga ctgcaaaatg taccaactga aaaaagaaag aataccgttg
      301 tgtgttttat ttctcgatca gcttttaaaa catggggtcc cgagcgccta cgaggaattt
      361 gtatcgataa gaaatagatt taaaaatttc gctgttattt tgtacattta acttgacggt
      421 gacatctctc tattgtgagt tattagtggt acagttttca ac
//
LOCUS       DACDYI010000218          450 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.228, whole genome shotgun sequence.
ACCESSION   DACDYI010000218 DACDYI010000000
VERSION     DACDYI010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.228"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
BASE COUNT          143 a           90 c           68 g          149 t
ORIGIN      
        1 gacactcctc ttaatataca gtcctattgt ataaagtgtt actatataca tagtaactgt
       61 atacataaaa aaagtcaagt attagaaaaa agttcaaaag atcaagtgtg cgcttcgctc
      121 ccactcccct cgacgtttta gtagcctttc cctcacttcg ttcagtccaa gccaactaaa
      181 aagttttcgg gatacattct ccttctcttc ctaataatca attaaaattt taatctttat
      241 atttctgcta atcactcagg aaacagtaca gaaaaacaaa cacgtatcat agatataaat
      301 gtaatggcat agtgcgggtt ttattttcag cctgtatcat agctaaacaa atcgagttgt
      361 gtgtccgttt ttgggcgttc tgctagcttg tttaaagtct cttgaatgaa tgtatgttct
      421 aagtcaaaaa actctgacag cgcctttata
//
LOCUS       DACDYI010000219          430 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.229, whole genome shotgun sequence.
ACCESSION   DACDYI010000219 DACDYI010000000
VERSION     DACDYI010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.229"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>430
                     /locus_tag="IWM52_002979"
     CDS             <1..>430
                     /locus_tag="IWM52_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HAP9975146.1"
                     /translation="LYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPD
                     NYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWF
                     SRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTP"
BASE COUNT          143 a           84 c           73 g          130 t
ORIGIN      
        1 cactttatcg tttattttct aaaggaatat ttgatattga cacactaccg atgaaaggta
       61 aaagaaaacc caatggccat caggaaaaac ggggaaaaca acagtatcag cgctcaatcc
      121 atgatcgacc tgataattat cctgatttca attctgagtt tggtcacctt gaaggtgata
      181 cgatcgttgg cattcatcat aaaagtgccg tcattacttt agttgaaaga ttatctaaag
      241 tcattatcac gattaaaccc aacggccgta aggcattaga tattgaaact gcccttaatc
      301 aatggttttc tcgcttccct aaaaacttct ttaaatctat tacgtttgac tgtggaaaag
      361 aattttctaa ctggaaagcc attagtaacc aacatgatat tgatatatat tttgcggacc
      421 ctggaacacc
//
LOCUS       DACDYI010000220          424 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.230, whole genome shotgun sequence.
ACCESSION   DACDYI010000220 DACDYI010000000
VERSION     DACDYI010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.230"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>424
                     /locus_tag="IWM52_002980"
     CDS             <1..>424
                     /locus_tag="IWM52_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HAP9975147.1"
                     /translation="KVGYNSTKKSYFYGLKIHMIVTKTGFPITYSITNPGVHDVKVLE
                     TLSEEANLPNILGDKGYISHKIHEKLALKGITISVPPRKNMDKSEKLDHSLLGKQRKT
                     VETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLL"
BASE COUNT          149 a           77 c           74 g          124 t
ORIGIN      
        1 caaagttggc tataactcaa caaaaaaatc ttatttttat ggtcttaaaa ttcatatgat
       61 cgttactaag actggctttc cgattactta ctcaatcacg aatccaggtg tccatgacgt
      121 gaaagtgtta gagactttat cggaagaagc gaatcttccc aatatcctag gggacaaagg
      181 atatattagc cataaaatcc atgaaaagct tgcccttaag ggcattacta tatccgttcc
      241 gccacgtaaa aacatggata aatcagaaaa actagaccac agcttacttg gaaaacaaag
      301 aaaaacagtt gagactgttt tttcttcctt agaaaaatta ggttgtcaaa atttcaactc
      361 acgttctgtt aaagggctag agagtagatt tgaaagcata ttactggctt acagtgttct
      421 atta
//
LOCUS       DACDYI010000221          392 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.231, whole genome shotgun sequence.
ACCESSION   DACDYI010000221 DACDYI010000000
VERSION     DACDYI010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.231"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>392
                     /locus_tag="IWM52_002981"
     CDS             <1..>392
                     /locus_tag="IWM52_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP9975148.1"
                     /translation="VTYLHKTKRDWQSMFTMFDEQAEWFGNIKGQSLPSLNIGGGFPF
                     HHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMMGAGKSTVLKMIAEA
                     HLAAGDFIWGFEKGKDFIPFLKEYNGIMV"
BASE COUNT          122 a           74 c           92 g          104 t
ORIGIN      
        1 gtgacttatc tgcataaaac caaacgagat tggcaatcga tgtttaccat gttcgatgaa
       61 caagctgaat ggttcggaaa tatcaaaggg caatcactac catcgttaaa cattggcgga
      121 ggtttcccgt tccaccacca aacgctaaaa gatccaggag gtacacaata tggggaaacc
      181 atgacaggcg gcccatttgt ctggaatgcg acgtataaaa atagtgtacg gttgtctact
      241 aataccttgc ttctaggaat gatgggagct ggtaaatcga ccgttcttaa aatgatcgcg
      301 gaagcccatt tagctgcagg agactttatt tggggctttg aaaagggaaa agactttatt
      361 cctttcttaa aggaatataa cggtattatg gt
//
LOCUS       DACDYI010000222          379 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.232, whole genome shotgun sequence.
ACCESSION   DACDYI010000222 DACDYI010000000
VERSION     DACDYI010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.232"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
BASE COUNT          116 a           65 c           78 g          120 t
ORIGIN      
        1 agttgtttta aatttaattg tctgctacgc ttgtgatgaa ttaattttcg ttctgcactt
       61 ttggacgata aatttggaca attgaaaata caaaaaaaag aaaggccctg tgagactcgg
      121 aaggctggta accttcgtcg acgtccacag gtggcattaa gacaaccttt aatcccttaa
      181 tgcgtttacc tttcagtgtt acggtattac ccgcaacgga tttagtataa cagatattca
      241 aaagtgatac cacttttttt ggctgtgtta aggttaaaaa tacgtgatat tacgatgaat
      301 ctatgcctaa gcataatgtt ataaaaatgt ccgtgtggct ctttgccgta tgacgaacgc
      361 tgaaattaga gggaacgca
//
LOCUS       DACDYI010000223          335 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.233, whole genome shotgun sequence.
ACCESSION   DACDYI010000223 DACDYI010000000
VERSION     DACDYI010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.233"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>335
                     /locus_tag="IWM52_002982"
     CDS             <1..>335
                     /locus_tag="IWM52_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9975149.1"
                     /translation="TQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDA
                     FEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSD
                     ADKYEPTVEG"
BASE COUNT          138 a           49 c           74 g           74 t
ORIGIN      
        1 actcaaccaa ttacagcaga tgatacagaa tttgtatcaa gtgcaacaaa actggatgat
       61 aaatctgacg ctgacaaata taatcctgaa ggtcaaaaag tgactacaga attgaataag
      121 gaaccagacg catttgaggg aattaagaac aaagaagatt taccaaaaga tactaagtat
      181 acttggaaag aaaaagtaga tgttagcgca gctggaaata aaaaagggac tgttgtagtg
      241 acatatccag atggatcatc tgatgaagtt gaagtagatg ttacagtgac agacaatcgt
      301 tctgacgccg ataaatatga gccaacagta gaagg
//
LOCUS       DACDYI010000224          330 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.234, whole genome shotgun sequence.
ACCESSION   DACDYI010000224 DACDYI010000000
VERSION     DACDYI010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.234"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(92..292)
                     /locus_tag="IWM52_002983"
     CDS             complement(92..292)
                     /locus_tag="IWM52_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9975150.1"
                     /translation="MAKAKTPFEKAASKILERRGVSETQWKQEVLEKAQFDLLRGKDK
                     DLEGYIYQREREKLVFDEIIKH"
BASE COUNT           91 a           67 c           52 g          120 t
ORIGIN      
        1 aatgctctaa aaatatcaac cgcagctact actttcggtt gtgttaaaaa attcattgta
       61 attgattcaa acataaaatt gttcgctcct tttagtgttt aataatttca tcgaaaacaa
      121 gcttttcacg ttcacgttga tagatatatc cttctaagtc cttgtctttt ccccgtaata
      181 ggtcaaattg tgctttttca aggacttctt gtttccactg tgtttcagat acaccgcggc
      241 gttctaagat tttagacgcg gctttttcaa atggcgtttt agcttttgcc actgtgattc
      301 ctccttcagg acaaataaaa agactagcaa
//
LOCUS       DACDYI010000225          330 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.235, whole genome shotgun sequence.
ACCESSION   DACDYI010000225 DACDYI010000000
VERSION     DACDYI010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.235"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            98..>330
                     /locus_tag="IWM52_002984"
     CDS             98..>330
                     /locus_tag="IWM52_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9975151.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMIIGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFA"
BASE COUNT          129 a           45 c           72 g           84 t
ORIGIN      
        1 caaaaaataa aaggtggatt aactgtcact gaaaaacaag tagatggtca atggaaagtc
       61 acagacttta gttatttcac tcgttaggag gaagaaaatg agtaagaaaa aaggagcaag
      121 acaacgtttc aaaaaacgaa aaagttatct tagatggctg ggaatcttta tgattatcgg
      181 ctttttaatt cttgctagtc cgatttatac gcataagaag gtagacatac cagatgaggt
      241 aagcacgaac gcatttttaa acacgaagga actgacgatg gtttcaaaag aaaaaggagc
      301 gaaccagcaa ttagtttttg aatttgcgat
//
LOCUS       DACDYI010000226          321 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.236, whole genome shotgun sequence.
ACCESSION   DACDYI010000226 DACDYI010000000
VERSION     DACDYI010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.236"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..>321)
                     /locus_tag="IWM52_002985"
     CDS             complement(<1..>321)
                     /locus_tag="IWM52_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9975152.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSL"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DACDYI010000227          312 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.237, whole genome shotgun sequence.
ACCESSION   DACDYI010000227 DACDYI010000000
VERSION     DACDYI010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.237"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
BASE COUNT          124 a           43 c           31 g          114 t
ORIGIN      
        1 ataagaataa aaatggttat aaaaacaaaa tacccgaact attagaagct atttatacat
       61 aaaaacataa attcaacact atttagtgat gaaaaaatat tttcgcttca atatagttcg
      121 gaatagttcg gatattttca attttatgaa tcatttcaat ttcttcataa ataagtgcag
      181 tttcttcttt ctctagcttt tgccattcat ctttcaaaat ataaataaaa tcaaatactt
      241 ttttattttt caatttgctg attccacaaa aaagataaag tccttataat tgtgtaaaag
      301 taaaaaggcc at
//
LOCUS       DACDYI010000228          306 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.238, whole genome shotgun sequence.
ACCESSION   DACDYI010000228 DACDYI010000000
VERSION     DACDYI010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.238"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            86..>306
                     /locus_tag="IWM52_002986"
     CDS             86..>306
                     /locus_tag="IWM52_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP9975153.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYAGKQKQIILKDPIPLK"
BASE COUNT          128 a           43 c           51 g           84 t
ORIGIN      
        1 catgaacgaa aatcaattct ctatttttga aaaataacaa gaaaaactta ttaaaaatat
       61 ttaaagtaag tgggtggatt attttatgtc atatacagga attttgagtc tcgaagatat
      121 ttgtcactac ggtaaacggt gtaccgctac agagaaaatc acgaagaaac tatcaactgg
      181 ccaaagtaaa accgttgtgc agtgtaagaa atatataatc cagaaagaca aagtctcaga
      241 ggaaatgatt tattacgctg gtaaacaaaa gcaaataatt ttaaaagatc ctatcccttt
      301 aaaaga
//
LOCUS       DACDYI010000229          287 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.239, whole genome shotgun sequence.
ACCESSION   DACDYI010000229 DACDYI010000000
VERSION     DACDYI010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.239"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>287
                     /locus_tag="IWM52_002987"
     CDS             <1..>287
                     /locus_tag="IWM52_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9975154.1"
                     /translation="FDYILDQYPPEHEWMKEKNKLFLNELYSKYQLPQTYQAFLESKE
                     ELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEERQQETDQEINEYATE"
BASE COUNT          115 a           46 c           50 g           76 t
ORIGIN      
        1 atttgattat attttggatc aatatccgcc agagcatgaa tggatgaaag aaaaaaacaa
       61 attattttta aatgagcttt attcgaaata tcaattaccc caaacctatc aagcattttt
      121 agagagtaaa gaagaattaa tctcacaggg taaaggcaca cttttagaac agttagagtc
      181 tgtccaatca acaaattggc atgagcttgc acaagaagat ttattagcca cctttgaaga
      241 acgtcaacaa gaaacagatc aagaaattaa tgagtatgcg acggaac
//
LOCUS       DACDYI010000230          283 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.240, whole genome shotgun sequence.
ACCESSION   DACDYI010000230 DACDYI010000000
VERSION     DACDYI010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.240"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>283
                     /locus_tag="IWM52_002988"
     CDS             <1..>283
                     /locus_tag="IWM52_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9975155.1"
                     /translation="EQATNTYKIEEVAKNASEKLKKNSSSAQAKIDKLNQTINEKNQE
                     IDQLKQQDGTKAKEEVNEAQSKTAEEFGKLAIDKSLSRNELTEKLKDVAT"
BASE COUNT          134 a           47 c           55 g           47 t
ORIGIN      
        1 gaacaagcga caaataccta taaaattgaa gaagtggcta aaaacgcgtc tgagaagctc
       61 aaaaaaaatt cctcttcagc gcaggcaaag atcgacaagt taaatcaaac tattaatgag
      121 aaaaaccaag aaatcgatca gctaaagcaa caagatggca caaaagcaaa agaagaagta
      181 aatgaagcac aaagtaagac ggcagaagag ttcggtaaat tggccattga taaatcatta
      241 agcagaaatg aattaacgga aaaactaaaa gatgtagcaa ccc
//
LOCUS       DACDYI010000231          265 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.denovo.241, whole genome shotgun sequence.
ACCESSION   DACDYI010000231 DACDYI010000000
VERSION     DACDYI010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.denovo.241"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(33..>265)
                     /locus_tag="IWM52_002989"
     CDS             complement(33..>265)
                     /locus_tag="IWM52_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9975156.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           77 a           39 c           47 g          102 t
ORIGIN      
        1 gaaggactga ccttctgtct cagatttttt tgttatttca cttgaatcaa ttgattgatc
       61 aaaaaaatcc tgattttcat gttctcaaat gatttaaatc cataagataa tcgtttcagt
      121 gtcttgatat ggggaatctt ggcctctatt ttgccattag aatacgggta aatcatagca
      181 ttgtaaatac cttcttcata cgttaaaagg ttttgaagtt tcgtgcgaag ttgtgcatca
      241 agtgtttcag gaagattttt taata
//
LOCUS       DACDYI010000232         7588 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.guided.101, whole genome shotgun sequence.
ACCESSION   DACDYI010000232 DACDYI010000000
VERSION     DACDYI010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.guided.101"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..1487
                     /locus_tag="IWM52_002990"
     CDS             <1..1487
                     /locus_tag="IWM52_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP9975157.1"
                     /translation="PHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDR
                     VESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIF
                     VFLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLI
                     ITTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSR
                     VMRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDG
                     KEKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVR
                     DLTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQ
                     YHDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTT
                     TADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            1484..2485
                     /locus_tag="IWM52_002991"
     CDS             1484..2485
                     /locus_tag="IWM52_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP9975158.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            2482..3414
                     /locus_tag="IWM52_002992"
     CDS             2482..3414
                     /locus_tag="IWM52_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9975159.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            3659..3775
                     /locus_tag="IWM52_002993"
     CDS             3659..3775
                     /locus_tag="IWM52_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP9975160.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            3791..5710
                     /gene="tet(M)"
                     /locus_tag="IWM52_002994"
     CDS             3791..5710
                     /gene="tet(M)"
                     /locus_tag="IWM52_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAP9975161.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNVCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            5829..5996
                     /locus_tag="IWM52_002995"
     CDS             5829..5996
                     /locus_tag="IWM52_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9975162.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(6056..6409)
                     /locus_tag="IWM52_002996"
     CDS             complement(6056..6409)
                     /locus_tag="IWM52_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9975163.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSIM
                     ESLASGINEARNIED"
     gene            6914..7336
                     /locus_tag="IWM52_002997"
     CDS             6914..7336
                     /locus_tag="IWM52_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9975164.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            7333..7563
                     /locus_tag="IWM52_002998"
     CDS             7333..7563
                     /locus_tag="IWM52_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9975165.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
BASE COUNT         2521 a         1335 c         1525 g         2207 t
ORIGIN      
        1 tgccccattc cgatagtcaa ggcaaggaca gcgtggactt aatcagagat agcctgtttt
       61 ccatacaggt tcagcaaccg tggctactgc ttcaatacaa cagttcagac attgaaagta
      121 tcggtattga ccgtgtggaa agcctgctct ccaccagccc agattccaac aatggcgaag
      181 acagagaaaa aattgttgcg gaagaaattg aagacagaag caataccaat ctaaccatta
      241 caaagaccat taaccgttta ggtacagtct tcttcctatt tgtcttcaat attgggattt
      301 ccatatttgt attcctatta acaggaatca tgattttctc gcaggtactt tttatcatct
      361 atgctatgtt tctgcctgtg agctttattt taagcatgat tccatcattt gatggtatgt
      421 caaaacgagc cataacaaag ctctttaata ccattttgac acgagctgga atcacattga
      481 ttattacgac agcatttagt atttcaacca tgctctatac cttatcggct ggttatccgt
      541 tctttttgat tgcttttcta cagattgtga cctttgcagg aatctacttc aagctgggcg
      601 atttaatgag tatgttttct ctacagagta acgattctca aagtgtggga agtcgtgtga
      661 tgagaaaacc tcgtatgctt atgcacgctc acatgcaccg tctacagcgg aaacttggac
      721 gttccatgac tactctaggg gctgggtctg ccattgttac aggtaaaaaa ggacagtcgg
      781 gttcggggag ttctgcaagg acacaagcag atcactcccg accagacgga aaggaaaaat
      841 caacacttgg aaaacgtatc ggtcaaacca tcggtacagt agctgatacc aaagacagaa
      901 tggtagacac tgctagtggt ttgaaagaac aggttaaaga tttgccgacc aatgcaagat
      961 atgcagtata tcaaggaaaa tccaaagtaa aagagaatgt ccgtgattta accagtagta
     1021 tttctcaaac caaagcggac agagccagtg gacgcaagga acagcaggaa caaaggcgaa
     1081 aaaccattgc gaagcgtcgc tctgaaatgg aacaggtcaa acagaaaaaa cagcctgctt
     1141 cttctgttca tgaaagaccg actacaagac aagaacaata tcatgatgaa cagacctcaa
     1201 aacagtctaa tattcagact tcatataagg aatctcaaca agccaaacaa gagcgtccag
     1261 cagttaagtc cgatttttca agtccaaaag tggaacgcca aggcaatacc gttcaagaaa
     1321 aaaccgttca aaagccagca acttcaacca ctacagcaga tagaacttca caacgtccaa
     1381 tcacaaaaga acgtccgtct actgttcaaa gagtaccact acaaaataca agaagtagac
     1441 caccaatcaa aaccgccacc attaagaaag tcggtaagaa accatgaagt tgaaaacttt
     1501 agtgattggt ggttctggat tattcttgat ggtcttctca ctgcttctgt ttgttgccat
     1561 tttattttca gatgaacagg acagcggaat ttccaatatt cattatggag gtgtgaatgt
     1621 ttccgcagaa gtgctggctc ataagcctat ggtagaaaaa tatgccaaag aatatggcgt
     1681 tgaagaatat gtcaacatac ttcttgcgat tatacaggtg gaatcgggcg gtactgcgga
     1741 agatgttatg cagtcctcgg aatccctcgg tcttccacct aattcattga gtacagaaga
     1801 atccattaag caaggtgtga agtatttcag tgaattatta gccagtagcg aaaggctcag
     1861 tgtagattta gaatcggtta tccagtccta caattatggt ggtggtttct tagggtatgt
     1921 ggctaatcgt ggaaataaat atacctttga actggctcaa agtttctcaa aagagtattc
     1981 aggtggcgaa aaagtgtctt accccaatcc catagccata cctatcaatg ggggctggcg
     2041 atacaactat ggcaatatgt tttatgtgca actggtaacg cagtatcttg tcacaacaga
     2101 gtttgatgat gatacggtac aagccatcat ggacgaagca ctgaaatatg agggctggcg
     2161 atacgtttac ggtggagctt ccccgactac ttcttttgat tgtagcggac tgacacaatg
     2221 gacgtatgga aaagctggaa ttaacttacc acgaaccgca caacagcaat atgatgtgac
     2281 ccagcatatc ccactatcgg aagcacaagc tggcgatttg gttttctttc attctaccta
     2341 taacgctggc tcttatatta ctcatgttgg gatatacctt ggcaataacc gtatgtttca
     2401 tgcaggcgac ccaatcggtt atgccgactt aacaagcccc tactggcaac agcatttagt
     2461 gggagcagga cgaatcaaac aatgagaaag gaagatttaa tgatgaaatt tagaaaaaat
     2521 cagaataaag aaaaacagat accaaaggaa aagaaacctc gtgtctataa ggtcaatcct
     2581 cataaaaagg ttgtgattgc cttgtgggta cttttagggc ttagtttcag ctttgcgata
     2641 ttcaagcact ttacagctat agatactcat actattcacg aaacaactat catagaaaag
     2701 gaatacgttg atactcatca tgtagaaaat tttgtagaga actttgcgaa agtctactat
     2761 tcatgggagc aatccgataa gtccattgat aatcgaatgg aaagtctaaa aggctatctg
     2821 acagatgaac ttcaagctct caatgttgat acagtacgca aagatattcc tgtatcgtct
     2881 tctgtaagag gatttcagat atggacggta gagccaactg gcgacaatga gtttaatgta
     2941 acctacagtg tagaccagct cattacagag ggagaaaata caaagaccgt ccactctgct
     3001 tatatagtga gtgtctatgt agatggttct ggaaatatgg tactggttaa gaatccgacc
     3061 attaccaaca tacctaagaa atcaagttat aaaccaaaag ccattgaaag tgaggggacg
     3121 gttgattcca ttacaaccaa tgaaatcaat gagtttttaa cgacgttctt caagctctat
     3181 cctacagcga cagccagtga actttcctac tatgtgaatg acgggatatt aaaaccaatc
     3241 ggaaaagagt acatctttca agaactggta aatcctattc acaatcgtaa ggataatcaa
     3301 gtcacggtat cgctgacagt ggagtatatc gaccagcaga ccaaagcaac gcaggtatct
     3361 caatttgatt tggtacttga aaagaacggg agtaattgga agattataga ataacaaata
     3421 ttggtacatt attacagcta ttttgtaatc acgtactctc tttgataaaa aattggagat
     3481 tcctttacaa atatgctctt acgtgctatt atttaagtat ctatttaaaa ggagttaata
     3541 aatatgcggc aaggtattat taaataaact gtcaatttga tagcgggaac aaataattgg
     3601 atgtcctttt ttaggagggc ttagtttttt gtacccagtt taagaatacc tttatcatgt
     3661 gattctaaag tatccggaga atatctgtat gctttgtatg cctatggtta tgcataaaaa
     3721 tcccagtgat aagagtattt atcactggga tttttatgcc cttttgggct tttgaatgga
     3781 ggaaaatcac atgaaaatta ttaatattgg agttttagct catgttgatg caggaaaaac
     3841 taccttaaca gaaagcttat tatataacag tggagcgatt acagaattag gaagcgtgga
     3901 caaaggtaca acgaggacgg ataatacgct tttagaacgt cagagaggaa ttacaattca
     3961 gacaggaata acctcttttc agtgggaaaa tacgaaggtg aacatcatag acacgccagg
     4021 acatatggat ttcttagcag aagtatatcg ttcattatca gttttagatg gggcaattct
     4081 actgatttct gcaaaagatg gcgtacaagc acaaactcgt atattatttc atgcacttag
     4141 gaaaatgggg attcccacaa tcttttttat caataagatt gaccaaaatg gaattgattt
     4201 atcaacggtt tatcaggata ttaaagagaa actttctgcc gaaattgtaa tcaaacagaa
     4261 ggtagaactg tatcctaatg tgtgtgtgac gaactttacc gaatctgaac aatgggatac
     4321 ggtaatagag ggaaacgatg accttttaga gaaatatatg tccggtaaat cattagaagc
     4381 attggaactc gaacaagagg aaagcataag atttcagaat tgttctctgt tccctcttta
     4441 tcatggaagt gcaaaaagta atatagggat tgataacctt atagaagtta ttactaataa
     4501 attttattca tcaacacatc gaggtccgtc tgaactttgc ggaaatgttt tcaaaattga
     4561 atatacaaaa aaaagacaac gtcttgcata tatacgcctt tatagtggag tactacattt
     4621 acgagattcg gttagagtat cagaaaaaga aaaaataaaa gttacagaaa tgtatacttc
     4681 aataaatggt gaattatgta agattgatag agcttattct ggagaaattg ttattttgca
     4741 aaatgagttt ttgaagttaa atagtgttct tggagataca aaactattgc cacagagaaa
     4801 aaagattgaa aatccgcacc ctctactaca aacaactgtt gaaccgagta aacctgaaca
     4861 gagagaaatg ttgcttgatg cccttttgga aatctcagat agtgatccgc ttctacgata
     4921 ttacgtggat tctacgacac atgaaattat actttctttc ttagggaaag tacaaatgga
     4981 agtgattagt gcactgttgc aagaaaagta tcatgtggag atagaactaa aagagcctac
     5041 agtcatttat atggagagac cgttaaaaaa tgcagaatat accattcaca tcgaagtgcc
     5101 gccaaatcct ttctgggctt ccattggttt atctgtatca ccgcttccgt tgggaagtgg
     5161 aatgcagtat gagagctcgg tttctcttgg atacttaaat caatcatttc aaaatgcagt
     5221 tatggaaggg atacgctatg gttgtgaaca aggattgtat ggttggaatg tgacggactg
     5281 taaaatctgt tttaagtatg gcttatacta tagccctgtt agtaccccag cagattttcg
     5341 gatgcttgct cctattgtat tggaacaagt cttaaaaaaa gctggaacag aattgttaga
     5401 gccatatctt agttttaaaa tttatgcgcc acaggaatat ctttcacgag catacaacga
     5461 tgctcctaaa tattgtgcga acatcgtaga cactcaattg aaaaataatg aggtcattct
     5521 tagtggagaa atccctgctc ggtgtattca agaatatcgt agtgatttaa ctttctttac
     5581 aaatggacgt agtgtttgtt taacagagtt aaaagggtac catgttacta ccggtgaacc
     5641 tgtttgccag ccccgtcgtc caaatagtcg gatagataaa gtacgatata tgttcaataa
     5701 aataacttag tgtattttat gttgttatat aaatatggtt tcttgttaaa taagatgaaa
     5761 tattttttaa taaagatttg aattaaagtg taaaggagga gatagttatt ataaactaca
     5821 agtggatatt gtgtcctgta tgtggaaata aaacacgatt aaagataagg gaagatactg
     5881 aattaaaaaa attccccctc tattgtccga aatgcagaca agaaaattta attgaaataa
     5941 agcagttcaa agtaactgtg attacagagc cagacgcaaa gacgcagagc cgataaaatg
     6001 agattaatac aatctcattt tatcggctct ttccgttatg tatggattct tttaattagt
     6061 cttcgatgtt tcttgcttcg ttgataccgc tggctaaaga ttccattatg gatagttctt
     6121 tgtctgtaaa gctatccatg tatttctcta tctgtaatcg tcgggtgctt tttaccaagt
     6181 tattagcagg taagaaaaat tcatcaacgg aaacatgaag taacgataca aggtcataaa
     6241 gaacttgtat gctggggtgt tgccctttat tttcaatatt agttaagtac cgtgggtcaa
     6301 tttcaatcaa tgctcccact tgttcacgag ttaaacctcg tttcaatcga gcttctttaa
     6361 tggctaaacc aaaggctcta aaatcatatt tatcttcttt tttacgcata gtagaccacc
     6421 tctatacatt ttattgttcc tactgaatta aaaacaggta tagaaaaacg tgttatatgg
     6481 tttataggtt tatatttaat aaaaagcact actaaacgcc aataaaaaaa accgttatat
     6541 ggtagtgcta tttacgctgt taaaatattg tatattactt ccaaatggcg gtttgttgga
     6601 ggtcaacgtc gccatgaagt acatcatata caataaattt ccttacattg ggttcttgtc
     6661 aaaaaaagtc gtctatctgc aatagataag tacgtccacc aatgtggttt tataaatcat
     6721 atagatagaa taacagaagc atgtaaacag agaaataaat ctgtttatat gcttttttgg
     6781 ctattcagaa cttttttaca aagtttattt atcagtaatg caacaaatcc ccctttcaca
     6841 ttgggactaa gagtgaaagg agataaacga gcaaggctca cttcctttcc tagacagaaa
     6901 gggggtgaga aacatgaaac catcttcttt tcagaccaca atagaaaatc agtttgacta
     6961 tatctgtaaa cgtgctatgg aagacgagcg aaagaattat atgctttatc tttcaaggat
     7021 tgcaaagcgt gaggtgtcct tttcggatgt tggcgattat cttgttagcc agtttgcgac
     7081 aacagataac tattcaactg actttcagat ttttacactc aatgggttat cagtaggcgt
     7141 tgaaaatgat ttgttgagtg aagcattacg tgagttgcca gacaagaaac gtgaaattct
     7201 actgctgttt tactttatgg acatgagcga ttcagaaatt gcagacctgt tgaaattgaa
     7261 ccgttctact gtctatcggc atagaaccag tggactagcc ttaattaaaa agtttatgga
     7321 ggaatttgaa gaatgaaaac acaatatcct atgattccct ttcctctcat tgtaaaggca
     7381 acagatggcg ataccgaagc gattaaccag attctacatc attacagagg gtacataacg
     7441 aagcgttccc tacgacttat gaaagatgaa tatggcaatc aaagtatggt cgttgatgaa
     7501 gtcttacgtg gaagaatgga aaccagactg attacaaaga ttttgtcatt tgaaattaag
     7561 taatatcctc tctcctttcg tggaagcg
//
LOCUS       DACDYI010000233         5839 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.guided.112, whole genome shotgun sequence.
ACCESSION   DACDYI010000233 DACDYI010000000
VERSION     DACDYI010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.guided.112"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            172..357
                     /locus_tag="IWM52_002999"
     CDS             172..357
                     /locus_tag="IWM52_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9975166.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            1300..1959
                     /locus_tag="IWM52_003000"
     CDS             1300..1959
                     /locus_tag="IWM52_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HAP9975167.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            1962..3305
                     /gene="vanS"
                     /locus_tag="IWM52_003001"
     CDS             1962..3305
                     /gene="vanS"
                     /locus_tag="IWM52_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP9975168.1"
                     /translation="MERKGIFIKVFSYTIIVLLLVVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQNKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLGNIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            3476..4282
                     /locus_tag="IWM52_003002"
     CDS             3476..4282
                     /locus_tag="IWM52_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HAP9975169.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            4300..5127
                     /gene="vanW"
                     /locus_tag="IWM52_003003"
     CDS             4300..5127
                     /gene="vanW"
                     /locus_tag="IWM52_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HAP9975170.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATIPEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            5124..>5839
                     /locus_tag="IWM52_003004"
     CDS             5124..>5839
                     /locus_tag="IWM52_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAP9975171.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTARGALVDTG"
BASE COUNT         1715 a         1191 c         1488 g         1445 t
ORIGIN      
        1 gccgggacaa tcttcaatat tcgccatatc gcacctccca cttacattgt atatttcata
       61 ctctgcccgt ggaatgttgg tatatgcacc tttcttaaag tttatatttc acattttgtt
      121 tcttttattc ttcgagcaaa agaattataa tgtaccctgt ggaggtgtac tatggactac
      181 atgacgttga aagaggcagg cgagaaatgg ggtgtgcccc cacgatggat aaactactat
      241 tgtgctgcgg gacgcatccc cggcgctgta aagatggcga cgatctggct cattcccaaa
      301 actgcggaaa agccgattga caggcgtaca aagaaaggaa aggcggttac tgaatagtat
      361 aatgaaaccg gcgcattttg gatatggttt tgaagagaga gaaagcatgg tattttgtca
      421 cggaaaaact ataagatgat agatggcagg ctgctccaga ccgataaaaa gtattctcat
      481 ctgaaacaga aagaaaagat ttacacatgg atgtatgaag aagcgaaact ttatcatgac
      541 agaaatggaa aatgcccgaa acaaagacga ggaagctgct gctgtatcag ccgtatatgg
      601 tagaattgaa aaggccggca tctggattct ttatggtgaa gtctccagac attggataac
      661 tgctggtcta atgcgccttt gagtatgggg tgattgattt taataccggg gaggtacggc
      721 tttttgagga aaatattctg ggatttacat ttggcgatta ttacatttcc tgtaactgca
      781 aggacacgga tgatgaagat atgaggaata tagacttgct gattttctac tgtcttgaac
      841 ggtattagga ctgaatctgc tgcgcggagt gcggcaagag cgaccggagg gagtacacac
      901 aggccctgcc tgtgtgaaaa gccataccgc aaggtgcatt cgcaaaaaag aggccgcgca
      961 ttttgctgag gcttggggtg tccccaacaa gcaaaacgag aacgccgcag gcgggcaagt
     1021 ggtggatcag aatatccgta aaggatattc tgatccattg cttgccagta ttatcgggat
     1081 gttttgacaa ccgctgtaat cgaaacatcc caaacattaa gaaggtgtta attattatta
     1141 tcttgattta attcgtttaa accaataata tagatacata caccatgaaa agaagcgagt
     1201 atcccaatga cggatgatta aaatccgaaa agaaatcgct ttgaaactac agggaaacta
     1261 cagactgtta tgttatcttc ttaaaatgga gggattttta tgtcgatacg aattctactt
     1321 gtcgaggatg atgatcatat ctgtaataca gtaagggcgt ttctggctga ggcaggatat
     1381 caggtggatg cctgcacaga tggaaatgag gcatacacca agttttacga aaacacttat
     1441 caactggtta ttcttgatat tatgctgccc ggtatgaacg ggcatgaact tttgcgtgaa
     1501 tttcgtgcga aaaatgatac tcccattctg atgatgacag ccctgtcgga tgacgaaaac
     1561 caaatccggg cgtttgatgc agaggcagac gactatgtaa caaagccatt caagatgcag
     1621 attttactaa agcgggtgga agccctgctg cggcgcagcg gtgcgctggc aaaggaaatc
     1681 cgtgtcggca ggctgacact tctgccggag gatttcacgg tactttgtga cggtacagag
     1741 ctgcccctga cacgaaaaga atttgaaatc cttttgctgc tggtgcagaa caaaggcaga
     1801 accttaacgc atgaaatcat tttgtcacgt atatggggat atgactttga gggtgatggc
     1861 agcacagtcc acactcatat caaaaatttg cgggccaagc tgccggaaaa tatcatcaaa
     1921 accattcgcg gtgtaggata ccgattggag gaatcataat aatggaaaga aaagggattt
     1981 tcattaaggt tttttcctat acgatcattg tcctgctact ggttgtcggt gtaacagcaa
     2041 cactgtttgc acagcaattt gtgtcttatt tcagagtgat ggaattacag caaacagtaa
     2101 aatcctatca gccattggtg gaactgattc agaatagcga taggcttgat attcaagagg
     2161 tggcagggct gtttcactac aataaccaat cctttgagtt ttatattgaa gataaagagg
     2221 gaagcgtact ctatgccaca ccaaatgcca atacatcaaa tagttttaga cccgactttc
     2281 tttatgtggt acatagagat gataatattt cgattgttgc tcaaaacaag gcaggtgtgg
     2341 gattgcttta tcaagggctg acaattcggg gaattgttat gattgcgata atggttgtat
     2401 tcagcctttt atgcgcgtat atctttgcgc ggcaaatgac aacgccgatc aaagccttag
     2461 cggacagtgc gaataaaatg gcaaacctga aagatgtacc gccgccgctg gagcgaaagg
     2521 atgagcttgg cgcactggct catgatatgc attccatgta tgtcaggctg aaagaaacca
     2581 tcgcaaggct ggaggatgaa atcgcaaggg aacatgagtt ggaggaaaca cagcgatatt
     2641 tctttgcggc agcttctcat gagctaaaaa cgcccatcgc ggctacaagc gttctgttgg
     2701 agggaatgct tggaaatatc ggtgactaca aagatcattc taagtatctg cgcgaatgca
     2761 tcaaaatgat ggataggcag ggcaaaatca tttccgaaat actggagctt gtcagcctga
     2821 atgatgggag aatcgtaccc atagctgaac cgttggacat agggcgcacg gttgccgagt
     2881 tgctgcccga ttttcaaacc ttggcagagg caaacaacca gcggttcgtc acagatattc
     2941 cagccgggca aattgtcctg tccgatccga ggctgctcca aaaggcacta tccaatgtca
     3001 tattgaatgc agttcagaac acgccgcagg gaggcgaggt acggatatgg agtgagcctg
     3061 gtgctgaaaa atgccgcctt tttgttttga acatgggcgt tcacattgat gatactgcgc
     3121 ttccaaggct gttcacccca ttctatcgca ttgatcaggc gcgaagcaga aaaagtgggc
     3181 gaagcggttt aggacttgcc atcgtacaaa aaacgctgga tgccatgagc ctccagtatg
     3241 cgctggaaaa cacctcggat ggcgttttgt tctggctgga tttaccgctc acatcaacat
     3301 tgtaaatatt taaaacttaa atgattttga ccgacaggta taaccctgcc ggtctttttt
     3361 tgctgctaca ggaaaactac agactggcta cagggaaagt acagatgcgc ttgccataat
     3421 aacaatcgta ccagccacaa atcgtagttt tattgcaaag gaggcattcg atcaaatgga
     3481 aaaaagcaac tatcattcca atgcggatca tcacaaacgg catatgaaac aatccgtgga
     3541 aaagcgggct tttctatgtg cgttcattat ttcgttcaca gtctgcacgc tgtttttggg
     3601 gtggagactg gcttccgtat tggaggcaac acagataccg cccatccctg caactcatac
     3661 aggcagcagc actgacgtag tggagaattt ggaggaaaac gctcttgcca ccgccaaaga
     3721 acagggagat gaacaggaat ggagcctgat tttagtgaac aggcagaacc ccatccccgc
     3781 acagtacgat gtggagcttg agcaactatc aaatggtgag cggatagata ttcggatttc
     3841 tccctatctt caagatttgt ttgatgccgc aagaactgat ggagtttacc cgattgtcgc
     3901 atccggatac cgaacaacag aaaaacagca agaaattatg gatgaaaaaa ttgccgaata
     3961 taaggcgaaa ggctacacct ctgcacaggc taaagcggaa gcagaaactt gggtggccgt
     4021 gccgggaacg agcgagcatc agcttggtct tgctgtggat atcaatgcgg acggaattca
     4081 ttcaacaggc aacgaggttt atagatggct ggatgaaaac agctatcgtt ttggttttat
     4141 tcgtcgctac ccaccagaca agacagagat aaccggtgtg agcaacgagc cgtggcatta
     4201 ccgatatgtt ggcatcgaag ctgccacaga gatgtacaac caaggggttt gccttgagga
     4261 atatttaaaa ccagaaaaat gagaaaagga tgttatgtta tggacagaaa aagattgaca
     4321 cagcgtttcc cgttcctgct tccaatgaga cgggcgcaga gaaaaatgtg cttttatgcg
     4381 ggaatgagat ttgacggctg tcgctatgcg cagacgatag gggaaaaatc gctttcccat
     4441 ttgctctttg aaacggattg cgcattatac aaccacaata ccggatttga catgatatac
     4501 caggaaaaca aggtgttcaa cttaaagctg gcagcaaaga ccttaaacgg cttattgata
     4561 agaccggggg aaaccttttc tttctggtgg ctggtacgcc atgcggacaa agataccccc
     4621 tataaagacg gccttacggt gaccaatggt aaactcacca ccatgtcggg cggcggtatg
     4681 tgccagatga gcaatttact attttggatg ttcctgcata cgccattgac aattatccag
     4741 cgcagaggtc acgaagtaaa ggagtttcca gagccaaaca gtgacgagat taaaggggtg
     4801 gatgcaacca ttccagaggg ctggattgat ttaaaagtgc gaaacgatac cgactgcacc
     4861 taccaaatat gggtgacctt agatgatgag aaaatcatcg gtcaggtgtc cgccgacaaa
     4921 gagccccaag cattatacaa aattacaaat ggcagtatcc agtatgtccg tgaaagtggc
     4981 gggatttatg aatatgccca ggttaaacgg atgcaagttg ccttaggtac cggggaaata
     5041 atagattgca agctgcttta tacaaacaaa tgcaaaatct gttatcccct cccggaaagt
     5101 gtggatattc aggaggagaa ccaatgagaa aaagtgtggg cattactgtt tttggatgcg
     5161 aacaggatga ggcaaatgct ttccgcgctt tatcgccgga ttttcatatt atccctacgc
     5221 tgattagcga tgcgatatcg gcagacaacg caaaattggc cgctggcaat caatgcgtta
     5281 gcgtaggcca taagtccgag gtttccgagg cgacaattct tgcgctgaga aaggtcgggg
     5341 taaaatacat ttctacccgc agcatcggct gcgatcacat tgatacgact gccgccgaga
     5401 gaatgggaat ctcggttggc acggttgcgt attcgccgga cagcgttgcg gattatgctt
     5461 tgatgctgat gctgatggcc atacggggtg caaaacccac catgcacgcc gtggcgcaac
     5521 aagatttcag attggatcgt atccggggga aagaactggg ggatatgact gtgggagtta
     5581 ttggaaccgg ccatatcggg caagcggtcg tcaaaaggct gcggggattt ggatgccatg
     5641 tgctggccta tgataacagc cgaaaaatgg atgcagatta tgtccagctt gatgagcttc
     5701 taaaaaacag cgatattgtt acgctccatg tgccgctttg tgcggatacc cgccatctga
     5761 tcggtcagaa gcaaattgga gagatgaagc aaggcgcatt tttaatcaac accgcgcgcg
     5821 gagcgcttgt cgataccgg
//
LOCUS       DACDYI010000234         4552 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.guided.121, whole genome shotgun sequence.
ACCESSION   DACDYI010000234 DACDYI010000000
VERSION     DACDYI010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.guided.121"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(114..1055)
                     /locus_tag="IWM52_003005"
     CDS             complement(114..1055)
                     /locus_tag="IWM52_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP9975172.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            1181..2302
                     /locus_tag="IWM52_003006"
     CDS             1181..2302
                     /locus_tag="IWM52_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9975173.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            2560..4062
                     /locus_tag="IWM52_003007"
     CDS             2560..4062
                     /locus_tag="IWM52_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP9975174.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            4129..>4552
                     /locus_tag="IWM52_003008"
     CDS             4129..>4552
                     /locus_tag="IWM52_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9975175.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVC"
BASE COUNT         1481 a          831 c          980 g         1260 t
ORIGIN      
        1 aacaagcaca attcttatgg catcatgaga gttgtgtttt ctttttgatc ttttattggg
       61 cttaaaaaaa gagaaccagc tgctgattca gctgattctc atcagagagc cttttattct
      121 gcgccaagga ttccttcttt tgcatggact aattgggtca aaaaggcaca atatggtgta
      181 gcaatatcat atttttgtcc ttttcgccaa atggcaccat tgatgtagtc gatttcagtc
      241 aaacgatggt ttttgatcag gtcttggtac atagatgggt aatgcaaccc gatgttgtca
      301 ggatcataag tagaagcaat atgttggtac acttcttctt gatctagtat gattccttct
      361 tttgctgcga tagaagcaaa ttcagatacg attgttcgaa ccatgctctc tgccgctttt
      421 tgcgcgccta gttcagcaat attacaatcc aaaatcgtgc ataaaccatt caatgttcca
      481 tttacacaag cttttcgcca aatggaataa cgaacgttat ggctatagat cggatttaaa
      541 tttgcttctt gaaagacttc tacaactttt tttgtaaatg cttcgccttc tggttccaag
      601 ttctctagtt cgatttctcc atcacctaac aatgtgactt ttccgggacc agctagacca
      661 gctgtccaca tcgtgatccc aaataagata tttttctttg gcacatattt ttccagtaca
      721 tcttcatgac ctaatccatt tagtaaacac aaaacataag tattgtcagt aataatggat
      781 tggatcgaac aaaacatatc atctaattga ttggcttttg ttaatgctac gatcaagtcc
      841 acgtgttcgt tgctttcgat gatttcttct ggtgaataga ttggcagttt tgcgaccact
      901 tctttaccgt taaagtcagc aatcaaccca tttgtacgaa tggcttcaat gtgagcaggc
      961 caacgatcga tcaagaggac ctcatttccg ctttgatgta gcatcaatcc gattcggctg
     1021 cccattgctc cggccccagc tattgcgatt ttcatgtttt ttctctcctt tattcaatga
     1081 gttccctttt agtatataga aaaatggaag aaaatagcat tgttttgttt gaaaaaaatg
     1141 aaaaaaagtg tcttacttaa gaagaaagtg agggatacag atggatattt tggcggcaat
     1201 tgattcattt aaaggaagtg cttcttcatt tgaactaaat caggcagttc taaatggctg
     1261 ggaagatcgt aaaggaagaa aaatcaatgt gccgattgca gatggcggag aaaatactgc
     1321 agaagcaatc tattacgcgc tcaggggaac atggcgtacc attgatggga tggatctctt
     1381 attccgtcag cgaaaaatca gctatttatt gacaacttgg caaggaaaaa acaccgcagt
     1441 catcgaatct gcacaagcat tggggttaga tctacttgat aaaccaacgg atcaaactat
     1501 ccgacttgct tccagttatg ccttaggaga acttattaaa gatgcactgc ttcaagaagt
     1561 agaacaaatt attgtcactt taggaggaag cgggtgtagc gatggtggtc taggattact
     1621 ccaatccttg ggtgcttctt tagaagggta cacagaagga aatcctttat tatctactaa
     1681 aaaacttcat tttgatcgaa gaaacgttct tgtttctaaa gtaaaattga ttatcgctgc
     1741 agatgttacc aatccatata caggtcaaca aggagctgct tggcaatttg gaagacaaaa
     1801 aggaggaata gatacgacgc tagctttctt agataaacaa gcaggaaaga ttgctcagca
     1861 aataaaagat ttctgggagg tggatttaga tgatattcca ggaagtggag cagcaggcgg
     1921 tattggtgga gccctttttt tgttgggagc gaagatggtt tctggtttcg atttagtcag
     1981 ccaaatcact ggattggaaa aaaagatcga acaagcagat gtcatcatta caggagaagg
     2041 gcggatcgat cagcaaacga tccacggaaa agttccatac ggtgtagcag tgcttgcaaa
     2101 aaaatatcga aaaccaataa tcgctatttg cggaagtaga gaccaagagc tagaaggact
     2161 ttctgatttt ctcccaattg tcttaagtat tcatttgggt ccaataggtt tagaagaagc
     2221 aatggaacac aggcaaacat tagaaaaaac aaaaatctta ggacagacac ttagcagatt
     2281 tttcaccatc ctcccgaagt agttgacaaa atcattttct ttatgctatc gtaatttcaa
     2341 atataggaca aagtgaggga tccgtgcatt gaaaaattaa gtcgattgat gaaagctgca
     2401 ttcagactgt accttgtgga gacaaggaag cagtgtgcag attttcggga ttggcttggt
     2461 tttttcgatg tatgggtctt tttgtgtgta tccaaccctt gcatcctctt tagccagttc
     2521 tgaaaatagg actggtcttt ttttacgagg tgatggaaaa tgtctaaaat cgaaataaaa
     2581 aatctgacat tcggctacga cagccaaggc acattattat ttgaacaagc aaatctaaat
     2641 tttgacacac aatggaaact aggacttatc ggacgaaacg gtcgaggaaa gacaacttta
     2701 ctgaatattc tacaaaacaa actaccttat caagggcaag taatccatca gcaagaattt
     2761 gcctatttcc cgcaacagac aaaagataaa gaacgtttaa cctattacgt gttaaatgat
     2821 attacggatt ttgagatatg ggaaatcgaa agagagctcc aattgatgca aacagatcct
     2881 gaaatcttat ggagagaatt cagcacacta tcggggggag agaagacaaa agtcctactg
     2941 gcacttttat ttgtggatga cactcatttc ccgttaatcg atgaaccaac gaatcatttg
     3001 gatatctctg gtagaaaaca agtagcggct tatttgaaaa agaaaaaaca aggcttcatc
     3061 gtggtcagcc atgaccgggg atttatcgat gaagtagtgg accatgtttt agcaatcgaa
     3121 aaaagtcaac tggaacttta tcaagggaat ttctctatct atgaagaaca gaaaaaactt
     3181 cgtgatgaat ttgaaatggc tcaaaatgaa aaattgaaaa aagaagtcag taggctaaag
     3241 aaaacagcag ctgaaaaagc cgaatggtct cgttcccgag aaggagataa aacaaagaaa
     3301 caagtcggat tcatcgatac tgaatctaga cgagtgaata aaggagcagt gggtgctgat
     3361 gctgcacgga cgatgaaacg atccaaagca atcgtgaatc ggatggagac ccagatcagc
     3421 gagaaagaaa aactattaaa agatatcgaa tatatcgatt ctttgacgat gaatagccaa
     3481 gcgtctcacc ataagcgact tttaagcgta gaagatcttc aattagggta tgaaaatctg
     3541 ttattcgagc caattcattt tacaatcgag cctcatcagc gggtggcgat ttcaggtcct
     3601 aacggtgcag gaaagtcatc cattatccat tatcttctgg gggcattcaa cggcaaggtt
     3661 ataggagaaa aataccagcc aaaacatctg agcattagtt atgcaagcca aaattatgaa
     3721 gacaatcgag gaacgttggc ggaatttgca gagaaaaacc aagtagacta ccaagcattt
     3781 ttgaacaacc tccgaaagct tgggatggaa agagatgttt ttcataacaa gatcgagcag
     3841 atgagtatgg gccaacggaa aaaagtggaa ttggctaaat ctttatcaca gccagctgaa
     3901 ctatatacat gggatgaacc attgaattat ttggatgtct tcaatcaaga acaattagaa
     3961 caactgatct tgaacgtgaa acctgccatg ttactagtgg aacatgatca aaccttcctg
     4021 gataaagtat ctactgagat tatttctctt gagagaatct aacccttgta atctcaagaa
     4081 caacaaggta tggttaaggg gaactttgcc aacgaaggag agaatcacat gctttatgta
     4141 acgagacacg gagaaacaac ttggaacgca caaggattag tttgcggacg ggcagatgtg
     4201 ccgctgactg aaaaaggaca gatgcaggca cagaaactag cagaaaaggt agtagatttg
     4261 cctgttccaa tcacgaaaat catccattcc cctcttcaac gagcaagaga tacagcacaa
     4321 gcagttgctg atcgtctcag cttgccgctg accgttgatg aacgattggt agaaatggat
     4381 tttggggatt atgatggact gccaagcaaa gatgagaact ttcaaaaagc acgtttagcg
     4441 ttcgctgtac gattcccaaa tggggagtca gtcttagatg tgtatgcacg gatcgttcct
     4501 ttattgaaag aatgcatcga agacgaagaa aatgtctatc tgttagtttg cc
//
LOCUS       DACDYI010000235         4151 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.guided.127, whole genome shotgun sequence.
ACCESSION   DACDYI010000235 DACDYI010000000
VERSION     DACDYI010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.guided.127"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            383..847
                     /locus_tag="IWM52_003009"
     CDS             383..847
                     /locus_tag="IWM52_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9975176.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            831..1460
                     /locus_tag="IWM52_003010"
     CDS             831..1460
                     /locus_tag="IWM52_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP9975177.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            1814..3292
                     /gene="msr"
                     /locus_tag="IWM52_003011"
     CDS             1814..3292
                     /gene="msr"
                     /locus_tag="IWM52_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP9975178.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            3339..3785
                     /locus_tag="IWM52_003012"
     CDS             3339..3785
                     /locus_tag="IWM52_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP9975179.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(3984..>4151)
                     /locus_tag="IWM52_003013"
     CDS             complement(3984..>4151)
                     /locus_tag="IWM52_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9975180.1"
                     /translation="LNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
BASE COUNT         1366 a          683 c          846 g         1256 t
ORIGIN      
        1 gtttttgaac accgtttact caaaaaaggt tgtgacaaca tcttgtcata acttttttat
       61 tactgtttaa gcatactgat ccaatttcta atattcgacg ggattcttgc aaaaggcgat
      121 ttcggaaatt tttggcggtg taaaattatc tactgcagaa gagctatctt ctatagtttg
      181 ataagaatca ataatcttgg ttttagcgtt gtgctttctt tatcattttt tccgttcatt
      241 ctacaaaaaa tttcttttct ctatactttt ctattgagag cgtttttttt attacacagc
      301 tgattctttg acttttttct ttttttattc tattattgac aagtcaatcg ttgacgagtc
      361 aattattaga aaggaggaaa ttatgtcttt agaagagatt agtcaccttt tatatcaaat
      421 caaattagcc gatcaaaaaa tcgtccagat ctttgaagaa aatatcggta taagtcttac
      481 tagatacgag atcatgatga gactgaaaga agaaaatcca ttgctacaaa gcagtttgca
      541 agaaaagttg cagattgatc aaggagcagt tactcgtcat ctgaaggtat tggaagcaaa
      601 tggctatgtt tcgcgtcaga gaaatcctca gaacaaccgg gaagtgtatg tagcgctaac
      661 agaaaaagcc cagaagaaaa tcaagcattg tgaaatggac cataaagata caggttccgt
      721 gctatccccg gctttcagcg atgtagaaat gaaacaattt ctaacacttt tagataaatt
      781 gaatacccaa ctttgcaata taacaacaga aacgaaagag gttaataata atggaacaaa
      841 aaaatgattt ttcaacaatt atgactggta gaagatccgt tcgcgtatat gatgaaacat
      901 acaaaatccc acacgaagaa atgctggaaa tgatcaaaga agcgacgact gcaccttctt
      961 ctgtcaatat gaaaccatgg cgctttgtgg tcgctgaaag cgaggaagca aaagaaatct
     1021 tgcgtccttt gatccgtttc aatacgagac aaaatgatac ttcttctgca atggtcatga
     1081 tttttggcga tatgcaatgt tacgaatatg gcgaagaaat ctacaaccaa gcttacgaat
     1141 cagggaagat gccaaaagaa gtcagagatc aacaattagc tgcgatcatt ccttattaca
     1201 agagtttttc aagagaagaa atgaacgatg tagtcaaagt cgattctagc ttagcggcta
     1261 tgcaattcat gttagttgcg cgtgatcatg gctatgaaac aaatccaatt ggtggattcg
     1321 aagcagatca gttggcagag gcatttggtt tggataaaga ccgttatgtt ccagttatta
     1381 ttttatcaat aggaaaagcg gtagaagaag gttacgagtc tgttcgttta gcaccagaaa
     1441 agatcactat gttcgaataa aaggttttca gttactattc agtagctatt gctttttttg
     1501 aatcgtgtgt ggtaacctaa taaaaaagga ggtcggaaaa atgactgcat cgatgaaatt
     1561 acgtttcgaa cttttgaata acaactaaat aaattcaaaa gacatgtata aacaacaatt
     1621 tcatggatgg agtatgtcca ttttttcgaa cgacagtaga ataggtgaaa gctatgacag
     1681 atttggtgat ttagtcatag ttatttcgtt cagagtagga aaagatgtga tgcagttttt
     1741 ttgcaaacag atttttctgc tcttttttag tatcgataat tgttgtttta catgaaatag
     1801 gagtgtaaaa catatggaaa atttagcagt aaatataaca aatctacaag tcagctttgg
     1861 caaccagcta gaactatcta ttgattctct tcgtgtctat cagcaagatc gtatagggat
     1921 cataggagaa aatggtgtcg gtaaatcgac tttgctcaaa ctaatagccg gtgaactttt
     1981 tcccgatcat gggaaaatcc aaacagagat caccttcaac tacctgcctc aattaaccta
     2041 tctcgctgaa gcaaaggacc taaatttgga attagccagt catttccagt taagactgga
     2101 agaaacttca gagcggaaat ggagcggagg ggaagaacga aagatcgagt tgatacgtct
     2161 tctttcttct tatgaacaag ggatgcttct agacgagcca acaacccatc tagatagaaa
     2221 aagtattgat cgactgattg aagagcttcg ttattattat ggtacgctgg tttttgttag
     2281 tcatgatcgc tattttctag atgagctagc atcgaaaatc tgggaagtaa aagacggaga
     2341 aatccgagag ttttcgggga attatagtgc ctatctcact caaaaggaat tggagaaaaa
     2401 gactcagcta cgagaagcag agtcgatcat gaaagagaaa aaacgattgg aaaaatcgat
     2461 ccaagaaaag aaaaaacaag cggaaaagtt ggaaaaagtg tccagtaaaa agaaaaagca
     2521 acaaatcaga ctggatcggt tgtcttcctc taaacaaaaa gacagtgtac aaaaagccat
     2581 ccaaaagaat gcgaaaacat tagagagaag actccaaaaa ataggagaaa caaccaagcc
     2641 gcagcagatg aaacaaatcc gttttccagt accaaaatct cttgaactcc acagccgcta
     2701 tcccatcatg ggacaaaatg tccaattgga aagaagtggg agaacattac tggtaaatgg
     2761 tgattttcag ttttctttag gtaaaaaaat cgcgattgtc ggtgaaaatg gttcaggtaa
     2821 gacaacctta ttagaacata tccgcaaaca aggagaagga atccttctct ctccgaaagt
     2881 aagctttcaa gtatatcagc aaaagggtta tcaaatgaca tctgaagaat ccatcattcg
     2941 ttttgtcatg agacaaacag agttttcaga atcgcttgtc cgtagtttgc tgaatcactt
     3001 agggtttgct caggaaactt tgacgaaacc gttatgtaca ttaagtgggg gagaagcgac
     3061 ccgtctgacg attgctttgc tttttactaa gccaagtaat gtgttgctgt tagatgaacc
     3121 gactaatttt attgatatgg caacgatcga agctttagag aagctaatgc aaatatatcc
     3181 gggaacgatt ttgtttactt ctcatgattc ttactttgtc gagcgtacgg ctgatgaagt
     3241 ttatgaaata aaagggcaga aaataaaaaa agtacttacg agaaattttt aaatgtgcta
     3301 cgatgatttt cgtgacaaat ttaaaggagt ggtacagatt gcctacaaat aaaaaagaag
     3361 gaataatttt tacaacaatg atgtgttttt tgatggtctt ggggatgagc gcgtataatt
     3421 tatggctgca tcaagatctt catttatcaa gtttgttgat tggtctagtg cctggattcg
     3481 tggtggcttt cattttggat gtcttcatca tcggtgtgct ggcgaaaaaa attgccttta
     3541 aactaccagt aaatccagaa agcaaggtcc aaatgatctt aactatttca tgcttgatgg
     3601 ttttaggaat ggtgacatgt atgtctttat tcggtgtgat catggaacaa ggaatacctg
     3661 atcaattatt ctctgcttat atgaatgctt ggaaaatgaa tgtgattgcc gctttaccgt
     3721 tgcagttgat catcgttggc ccgctttcaa gaagtgtgct aaaagcaatt caaagtaatg
     3781 aatgatggaa cactgttatt tgaaaaaagg ccttggcatg ctttttgtca tagtctcttg
     3841 aatgcagttc tagaaaaata gtgatccgaa cgataggttt tggcctgtcg ttcggatcgc
     3901 tttttctttt ccaactgtat tgctctttta ttgaagataa tttgctcttc aatagatatt
     3961 tctagaaagg ttctgtttat cccttactca aaaaactgct gcatttgttc tcttacttgc
     4021 tgacaccatt ggagttcctg ctgacaaagc ataattctgc gctggatcac aacataacgt
     4081 ccaaattctt gtttcttgtc tagcgagagg tcattcaatt ttttttgatt tttttgcatg
     4141 attttgttca g
//
LOCUS       DACDYI010000236         3814 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.guided.133, whole genome shotgun sequence.
ACCESSION   DACDYI010000236 DACDYI010000000
VERSION     DACDYI010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.guided.133"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            183..1250
                     /gene="rlmN"
                     /locus_tag="IWM52_003014"
     CDS             183..1250
                     /gene="rlmN"
                     /locus_tag="IWM52_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP9975181.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(1443..2546)
                     /locus_tag="IWM52_003015"
     CDS             complement(1443..2546)
                     /locus_tag="IWM52_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9975182.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            3099..3647
                     /gene="aac(6')"
                     /locus_tag="IWM52_003016"
     CDS             3099..3647
                     /gene="aac(6')"
                     /locus_tag="IWM52_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP9975183.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         1247 a          648 c          764 g         1155 t
ORIGIN      
        1 atcaattctg attcatcagt cccataaatt atagagccat cttttctaat acggtatgat
       61 gtcttgtctt tttagttttt gtgagattaa agttctattc gatttatttg tgaagtattg
      121 cattaaggaa atgaatcatg tatagtatgt agcgaaactt tgagaaaaag agggaaaata
      181 ctttggaaaa acagtccatt tatggcttga cgaatgaaga attaatcaat tggtttatag
      241 aaaacggcga gaaaaaattc cgagctgccc aagtttggga atggttgtat caaaaacgtg
      301 taagtaattt cacagaaatg acgaatctat cgaaacaatt gatcgaaaaa ttatcggagc
      361 attttatcat caatcctcta aaacagatgg tcgtccaaga agcttcggat ggtacagtaa
      421 aatatttgtt tgaactccct gataaaaaca tgattgagac tgtactgatg agacaagaat
      481 atggcttgtc agtttgtgtg acgacacaag taggatgtaa tatcggctgt acgttttgtg
      541 caagcgggct gctcaaaaag aaccgtgatc tgactgctgg agaaattgtt gcacagatca
      601 tgatggtcca acattatttt gatgagcgca atcttggcga acgcgtttca catgtcgttg
      661 taatggggat cggtgagcca tttgataatt acgacaatgt aatggacttt ttgcatatca
      721 tcaatgatgc caaaggattg gcaattggtg ctcggcacat tacagtttcc actagtggat
      781 tagcacacaa aatcaaggaa ttcgctaata acggcttgca agtcaatctg gctatttcct
      841 tgcacgcgcc gaataatgag gtgcgaacat cgatcatgcg gatcaaccga agtttcccaa
      901 ttgagaaatt gatggaagct gtggatgagt atttagagaa aacaaaccgt cgaatcacgt
      961 ttgaatatat catgttgaat caagtaaatg atagaccaga gcatgcgcaa caattagcag
     1021 atctattaaa agataagaaa aaattagcat atgtcaactt gattccatat aatccagtca
     1081 gtgaacatga tcaatattct cgcagtccaa aagctgatgt attgagattt tatgatgtat
     1141 tgaagaaaaa cggcgtaaac tgtgtgatac gtaaagaaca tggtacagat attgatgcag
     1201 cttgcggaca attgcgaagc aagcaaatga aaaaaacagt tgccaaataa tcgtatccta
     1261 ctcgtttttt caattaccat aaaaggagca gttttcttaa ttaaggaact gttcaactta
     1321 aataagagaa aagattctat agcgaaagct ataaagcttt ttttgacaag aaaaaccctc
     1381 atcaagcaaa gaaatgtcct caatttgatg acagcctgct tgatgggggt tttagcaatt
     1441 atttaactga ttttcttata tccttgctgg ctttcttgtg tagcatgata agtttctacg
     1501 tcaagaatgg cctgtttaaa tttttctcgg gtgatttcat aaggcacatg tttaatatca
     1561 tttgttgcca tggctttatc taagattgct tctaaatcgg tcgtgacatt gagattcatg
     1621 tcggttaacg atactggaag ttggttcttt ttcataaaca catagatttt atcaaacaaa
     1681 tcgaactgct gatccatcat caataaggtc agtacaccat aactgacaga tgctccgtga
     1741 aggtatccct ccattttttc tagaacagtc gtaccgtaat agaatgagtg tgccatagca
     1801 gaattatagt tattgttaac taaaacagaa accaatccgg tagtaacaat gatatccgaa
     1861 acgacttgtt caattgcata tgagggacga tcgtcggcac tatctttcaa tgcttgcgta
     1921 ccataagtca agagacgatc aatgcacatt tttccgattt ggattcctaa tctatccgtg
     1981 tagcctagat catcgccacg agcagaaaaa gtagattcga cttctttgga taaagcatcg
     2041 ccaatacctg cccataagta tttatcaggt gcttcggcaa taattgctgt atcaatgaag
     2101 gtgtgtacag gtggttttga aggaaagaag agtccgtcca ttacgccttc ttcgttatag
     2161 atgacacata ccgcagtgac gggcgaacag ttcgaaccaa tagtgggaaa ggtgaagact
     2221 ggtttagcta gtttttcacc aatgactttt acagtatcgc aagcacggcc accaccgact
     2281 gcaaacagga cgtctgcttt ttggacactt tcatgctgga tcaactgttc aatatttcca
     2341 taagttgaat tacccccgta ccagagactg gcgacaacct ctatttcctg tgtcaaagct
     2401 acttttattt tgttttctgc ctttgaaagt gcagtcttcc ctccaatcag cactactttt
     2461 gtcccatatt ctttgacgat tccatttgct ttttcatacg catctgttcc aatggtataa
     2521 tttggtaaga aaacactttg actcatattc atttccttct ttcttcgttg aaacgggtta
     2581 aaattttaaa gagtgatcga tctgactgcg gctgtagatg gagtagaggt gaacctaaga
     2641 caggataagt gatgaataag gtggcatgaa caccttgaaa tcatcaataa gtatcctaaa
     2701 aacgaacaag tacaaaaaca ttgaaaacga caataatgaa tttttgttcc tcttagtatt
     2761 tctaccgatt ccctttgctt ttttgtaatt atctaaaaat tctaaaaatt aagattatta
     2821 tatgaaaaca ggttttattt gtcaattcat aattggtttt ttttaaagca aaagataatc
     2881 tatatcatga tgaacaaaca gagttttttc ctccaattga tggctttcct gatctttcct
     2941 attttgaata aagtgtttct gtataatttt tttattttat aagtaagata aataatgaaa
     3001 gaatagagat actgattggt agctaaagat ttttgagcga gaacatttcg ttttttcaaa
     3061 aggactgtta gaataagcaa gaacaagggg gaagacgtat gataatcagt gaatttgacc
     3121 gtaataatcc agtattgaaa gatcagcttt ctgatttact gagactgact tggccggaag
     3181 aatatggaga cagctcggca gaagaagtag aagaaatgat gaatccagaa cgaatcgcgg
     3241 tagcagcggt agaccaagat gagttagtag gatttattgg tgcaatccct caatacggta
     3301 tcacaggttg ggaattgcat ccattagttg tagaaagctc ccgacgaaag aaccaaatag
     3361 gtactcgatt agtcaattac ttagaaaaag aagtagcttc cagaggagga atcacgattt
     3421 atttaggtac ggatgattta gaccatggaa caacgttaag tcaaacggac ctgtatgagc
     3481 atacatttga taaagtggct tccatccaga accttcgtga acatccgtat gaattctatg
     3541 aaaaattagg ttataaaatc gtaggtgtct taccaaatgc aaatggctgg gacaaaccgg
     3601 atatttggat ggcaaaaacg attattcctc gaccaaattc tcaataataa aaagaggttg
     3661 tgacagaagt aatcagcttc aagaaataag aaggaattta cgaaaattac ttcttgaatt
     3721 tttgtgaaat tccaacttat ttccgaagaa gctacttttg gaacaccgtg taccagaggt
     3781 tgtgaaaaag tcgcttagtt tcgagtagta agaa
//
LOCUS       DACDYI010000237         3426 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.guided.136, whole genome shotgun sequence.
ACCESSION   DACDYI010000237 DACDYI010000000
VERSION     DACDYI010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.guided.136"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..450
                     /locus_tag="IWM52_003017"
     CDS             <1..450
                     /locus_tag="IWM52_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HAP9975184.1"
                     /translation="MCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPNSDEIKGVDAT
                     IPEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNGSIQYVRESGG
                     IYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            447..1418
                     /locus_tag="IWM52_003018"
     CDS             447..1418
                     /locus_tag="IWM52_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAP9975185.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTARGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            1411..2439
                     /gene="vanB"
                     /locus_tag="IWM52_003019"
     CDS             1411..2439
                     /gene="vanB"
                     /locus_tag="IWM52_003019"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HAP9975186.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKFG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQIIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            2445..3053
                     /locus_tag="IWM52_003020"
     CDS             2445..3053
                     /locus_tag="IWM52_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HAP9975187.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
BASE COUNT          936 a          719 c          928 g          843 t
ORIGIN      
        1 atgtgccaga tgagcaattt actattttgg atgttcctgc atacgccatt gacaattatc
       61 cagcgcagag gtcacgaagt aaaggagttt ccagagccaa acagtgacga gattaaaggg
      121 gtggatgcaa ccattccaga gggctggatt gatttaaaag tgcgaaacga taccgactgc
      181 acctaccaaa tatgggtgac cttagatgat gagaaaatca tcggtcaggt gtccgccgac
      241 aaagagcccc aagcattata caaaattaca aatggcagta tccagtatgt ccgtgaaagt
      301 ggcgggattt atgaatatgc ccaggttaaa cggatgcaag ttgccttagg taccggggaa
      361 ataatagatt gcaagctgct ttatacaaac aaatgcaaaa tctgttatcc cctcccggaa
      421 agtgtggata ttcaggagga gaaccaatga gaaaaagtgt gggcattact gtttttggat
      481 gcgaacagga tgaggcaaat gctttccgcg ctttatcgcc ggattttcat attatcccta
      541 cgctgattag cgatgcgata tcggcagaca acgcaaaatt ggccgctggc aatcaatgcg
      601 ttagcgtagg ccataagtcc gaggtttccg aggcgacaat tcttgcgctg agaaaggtcg
      661 gggtaaaata catttctacc cgcagcatcg gctgcgatca cattgatacg actgccgccg
      721 agagaatggg aatctcggtt ggcacggttg cgtattcgcc ggacagcgtt gcggattatg
      781 ctttgatgct gatgctgatg gccatacggg gtgcaaaacc caccatgcac gccgtggcgc
      841 aacaagattt cagattggat cgtatccggg ggaaagaact gggggatatg actgtgggag
      901 ttattggaac cggccatatc gggcaagcgg tcgtcaaaag gctgcgggga tttggatgcc
      961 atgtgctggc ctatgataac agccgaaaaa tggatgcaga ttatgtccag cttgatgagc
     1021 ttctaaaaaa cagcgatatt gttacgctcc atgtgccgct ttgtgcggat acccgccatc
     1081 tgatcggtca gaagcaaatt ggagagatga agcaaggcgc atttttaatc aacaccgcgc
     1141 gcggagcgct tgtcgatacc ggggcgctgg tggaggcgtt ggaaagcgga aagctgggtg
     1201 gtgcggcact ggatgtgttg gagggcgagg atcagtttgt ttataccgat tgctcgcaga
     1261 aagtgcttga ccatcccttt ttgtcgcagc ttttaaggat gccgaatgtg atcatcacac
     1321 cccatacggc gtactacact gagcgtgtgc tgcaggatac tacagaaaaa acaatcagga
     1381 attgtctcaa ttttgaaagg agtttacagc atgaatagaa taaaagtcgc aatcatcttc
     1441 ggcggttgct cggaggaaca tgatgtgtcg gtaaaatccg caatagaaat tgctgcgaac
     1501 attgatacgg aaaaattcga tccgcactac atcggaatta caaaaaacgg tgtatggaag
     1561 ctatgcaaga agccatgtac ggaatgggaa gccgacagtc tccccgccat actctccccg
     1621 gataggaaaa cgcatgggct gcttgtcatg aaagaaagcg aatacgaaac acggcgtatt
     1681 gatgtggctt tcccggtttt gcatggcaaa ttcggggagg atggtgcgat acaggggctg
     1741 tttgtattgt ctggtatccc ctatgtgggc tgtgatattc aaagctccgc agcttgcatg
     1801 gacaaatcac tggcctacat tcttacaaaa aatgcgggca tcgccgttcc cgaatttcaa
     1861 attattgata aaggtgacaa gccggaggcg ggtgcgctta cctaccctgt ctttgtgaag
     1921 ccggcacggt caggttcgtc ctttggcgta accaaagtaa acggtacgga agaacttaac
     1981 gctgcgatag aagcggcagg acaatatgat ggaaaaatct taattgagca agcgatttcg
     2041 ggctgtgagg tcgggtgtgc ggtcatgggg aacgaggatg atttgattgt cggcgaagtg
     2101 gatcaaatcc ggctgagcca cggtatcttc cgcatccatc aggaaaacga gccggaaaaa
     2161 ggctcagaaa atgcgatgat tacagttccc gcagacattc cggtcgagga acgaaatcgg
     2221 gtgcaggaaa cggcaaagaa agtatatcgg gtgcttggat gcagagggct tgcccgtgtt
     2281 gatctttttt tgcaggagga tggcggcatc gttctaaatg aggtcaatac catgcccggt
     2341 tttacatcgt acagccgcta cccacgtatg gtggccgccg caggaatcac gcttcctgca
     2401 ctgattgaca gcctgattac attggcgtta aagaggtgat aagtatggaa aatggttttt
     2461 tgtttttaga tgaaatgttg catggcgtcc gttgggatgc caagtacgcc acatgggata
     2521 acttcacagg aaaaccggtg gatgggtatg aggtgaatcg catcatcggc acaaaggccg
     2581 tggcgtttgc tctgcgcgag gcacaaatcc atgcggctgc gcttggctat ggcttgcttt
     2641 tatgggatgg atatcggcca agaactgcgg tggactgctt cctgcgttgg gcagcgcaac
     2701 cggaggacaa tctcacaaaa gaaaaatttt accccaatat agagcgagcc gagttgatta
     2761 caaagggtta tgtggcctca caatccagcc atagccgtgg aagcgcaatt gatcttacgc
     2821 tctaccacct ggatacaggg gaacttgttt caatgggaag taacttcgat tttatggacg
     2881 aacggtcgca tcatacagca aaagggatag gggatgcaga ggcacaaaat cgaagatgct
     2941 tgcgtaaaat catggaaagc agcggatttc agtcttatcg ctttgaatgg tggcactata
     3001 agttgattga tgagccatac cccgatacct attttaattt tgccgtttca taatgaaagt
     3061 attggttttg tatcagattt tggatttgat aaaatctatg ctggtcatgg acaatctttt
     3121 actggaaagt ggttaaagca tcttttgtaa tacattgcaa attatagtat tcaatctgcc
     3181 cagcggcaga aaagaaaaaa accgctgttg ggcagacctt tcccacgccc attttgtatt
     3241 ttgctggcgg cggttttgag aaatcaaggc cgccgttctt ttctggcagc aaaaggtgtg
     3301 gcggcccata ggaggatgcc gctatgctgt tttgctattg ccatgctcta atccacttcc
     3361 accactcaaa aaagccttgc ccctccatga actgaacccc aaaagttgaa ctatttaatg
     3421 gactgt
//
LOCUS       DACDYI010000238         2125 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.guided.159, whole genome shotgun sequence.
ACCESSION   DACDYI010000238 DACDYI010000000
VERSION     DACDYI010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.guided.159"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            24..551
                     /locus_tag="IWM52_003021"
     CDS             24..551
                     /locus_tag="IWM52_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9975188.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(614..1267)
                     /locus_tag="IWM52_003022"
     CDS             complement(614..1267)
                     /locus_tag="IWM52_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP9975189.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            1399..>2125
                     /locus_tag="IWM52_003023"
     CDS             1399..>2125
                     /locus_tag="IWM52_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9975190.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEF"
BASE COUNT          784 a          381 c          427 g          533 t
ORIGIN      
        1 ttagaatcag aaaggatgag aaaatgttag ctgcaatttg ggcacaggat gaaaacggat
       61 tgattggaaa agaagaccgt cttccttggc gtttgccaaa tgatctgaaa ttcttcaaac
      121 aaatgacaga agcaaataca ctagtgatgg gacgaaaaac attcgaaggg atgggtagcc
      181 gtccgttgcc gaatcgtcag acgattgttt tgacgagaga ttcttcctat aaagcagaag
      241 gcgtgcatgt catgcatagt gtggaagacg tgcttgctct ggaaaaagaa acagacggta
      301 tctttttcat cgctggaggg tcagctgttt atcaagaatt tctgccattt tgtacgatat
      361 tataccgtac agtcatccat catgtattcg atggagatgc ttattttcca cctgttgact
      421 ggtctgattg gtcactgatc aatactagtc aaggagaaat ggatgaaaaa aatcgttatc
      481 cgcatcaatt tgaaacgtat cagcgtaatg agaatcctgt aaaaagcggg acaggcgaat
      541 tgacgatata aaaaagctat ggatagacta gctgttattt agagctgtcc catccatagc
      601 tttatttttt ttattaagta aagaataaaa cagagaagta cataaaacct gcacctaaca
      661 taacaaacaa atgccaaaca acatgcatat agcggataga attcaagcta taaaagaatg
      721 cgcctatcgt gtaagaaagc ccgcctgcta ccatcaaaat aacaccagtc attccaagag
      781 agtgataaag aggaacagca gccacgacac agagccaccc cataaaaata tagatgatcg
      841 tagatacttt tgatactctc tcccgtttat gcaacgtcaa cgctttatat ataatgccaa
      901 gaatcgcaaa caaccagatt aatacaaata aagtccaacc tagccatcct cggatactca
      961 gcaaacaaaa aggagtataa cttcctgcaa tcaaaagaaa aatcgaacta tgatcgaata
     1021 cttggaacac cttttttgct tttgtaaaaa tcaatgaatg aaacaaagta gacgttaaaa
     1081 ataaaagaat catcatcgat ccatagatag cataagagac aatatggagg gaggaatgca
     1141 agtgggcacc ttttactaaa agaaccacta gtcccgcgat actcaatcca gtcccaattc
     1201 catgggtgac tgcattaaac acttcattta atatttcata cgttcgatta aatttcgttt
     1261 tttccatgaa aacggctcct ttactaggtg taattaatga aaaatcatta tttgtctgtt
     1321 atactaaaaa tagaaattgt cttacatgta gtttaacatg aataaggcat cttgttaaag
     1381 aaagagagag agaaaataat gacaaatgta aaaatcgtaa cagattcttc atgtacgatg
     1441 gaaaaaaatg tacgcgatgc tttaggcatt cgtgtcatgc cgctatccat catgatcgac
     1501 ggaacagtat atcccgatga tgaccaacta gacggcgaaa gatttatgga aatgatggcg
     1561 aaagcaaaaa atctgcccaa aacaagccaa ccaccaattg gagaattcat ctcgatgtat
     1621 gatgagctag gagaagacgg cagtgaagtg atttcgatcc atatgacaaa aggcttgagt
     1681 ggaacagtag aagcagcaag acaagccagt aatctaacaa aaacgaaagt aacggttatc
     1741 gacagtgata ccactgacca aggattatct tttcaggtaa ttcgagcagc tgaattagca
     1801 aaagaaggta aaacagtaga gaaaatcctt gaagaagtca aaaaagtaca agacaataca
     1861 aaattataca tcggaatatc cacactagat aacttggtaa aaggtggaag aatcagccga
     1921 acaactggtt tgctgtctag tgttttgaac atacgcgtgg tgatgaattt cgcccattct
     1981 gaattaatcc ctgtaactaa ggggcgagga aagaaaacgt tcgataaatg gtttaataat
     2041 ttgaaaaatg aactgaaaga attgccaaat gtgcgacaaa tcggtatttc acatgcggat
     2101 gcaaaagaat tggcgcaaga attca
//
LOCUS       DACDYI010000239         1658 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.guided.169, whole genome shotgun sequence.
ACCESSION   DACDYI010000239 DACDYI010000000
VERSION     DACDYI010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.guided.169"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            808..1305
                     /gene="dfrG"
                     /locus_tag="IWM52_003024"
     CDS             808..1305
                     /gene="dfrG"
                     /locus_tag="IWM52_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP9975191.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(1377..>1658)
                     /locus_tag="IWM52_003025"
     CDS             complement(1377..>1658)
                     /locus_tag="IWM52_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP9975192.1"
                     /translation="LNVNDHSTNSFIQQIRRYISSLERPLTTARGDKKSYIYANFNPK
                     YAQFAITILRTYYNFCRPFKSADKKVLTPAQRLGITDKQFDWKDIIYFK"
BASE COUNT          593 a          201 c          300 g          564 t
ORIGIN      
        1 gatagaaatt acaacaaaat atactccaac tgctctcatg gctttatgga tcagtattct
       61 gacttattgt ttagtggtgt attcaagttt taaacgattt gcacctaata agttgaaaga
      121 ttctcgatat gtgactaaaa aggagataaa caagaagaat gaaaataaat aaaaaagtag
      181 taatattgag tggattaatt atagttagta gtatatattc tttcatcttt ttcaaaaata
      241 cacagagtat ttataccact ggagatcttt cttttcactt atctcgaatc aaggggttat
      301 cgactatttt taccagtccg attaattatg agacattcaa ttatactgga tatggtgtaa
      361 attattttta tccctttctg actttttttc cagctgtaat gttatattgg atgacaaaaa
      421 atttgattgt aagctatatt atttacgttt ggttattgaa cttgtgtaca acactcgtag
      481 cttatcatta tggcgagcgg tttttaaaac aaaaaaaagc ggcattttta ttttaagtag
      541 ttcagttttg gagtacaaaa tgacaaaatg tacttcaaaa tggactactt ttttcatttt
      601 aacttacata ccaaattaca tacaaaatga tttataaaat gcaattgaaa atgataattg
      661 aatgaaaatt tgtgattttg tggtattatg ataatataac ttatagaaaa aattgcttat
      721 tcaactaacg ggcaggataa tgagaaaagg ttttttgata gaaataagaa attagatggt
      781 aaattagtta aaaaggctgt gttataaatg aaagtttctt tgattgctgc gatggataag
      841 aatagagtga ttggcaaaga gaatgacatt ccttggagga ttcccaagga ctgggaatat
      901 gttaaaaata ctacaaaggg acatccgata atattaggta ggaagaacct tgaatcaatc
      961 ggaagagcct tacctgacag aagaaatatt attctgacga gagataaggg gtttaccttt
     1021 aatggttgtg aaattgttca ttcaatagaa gatgtttttg agttatgtaa aaacgaagaa
     1081 gaaattttta ttttcggagg agaacagatt tataatttgt ttttccctta tgttgagaaa
     1141 atgtacatca caaaaataca tcatgaattc gaaggagata ctttttttcc agaagtgaat
     1201 tatgaggaat ggaatgaggt atttgcccaa aaagggataa agaatgataa aaatccgtat
     1261 aactactatt ttcatgtata tgaaagaaaa aacttattga gttaacgggt gcgttaattg
     1321 aagaagaaca gtaaagataa agcacaagag cttgggatta ctccaagctc tttttgtcat
     1381 ttgaaataaa ttatatcttt ccaatcaaat tgtttatctg taatccctaa tctttgagca
     1441 ggtgtcaaaa cctttttatc cgctgattta aaagggcgac aaaagttgta gtaagttctt
     1501 agaattgtaa tcgcaaattg agcatacttc ggattgaagt tagcgtaaat atagctcttc
     1561 ttatcaccac gagctgttgt taatggtctt tcaagagatg atatataacg tcttatctgt
     1621 tgaataaagc tattggtcga atggtcatta acatttaa
//
LOCUS       DACDYI010000240         1467 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.guided.178, whole genome shotgun sequence.
ACCESSION   DACDYI010000240 DACDYI010000000
VERSION     DACDYI010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.guided.178"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..1348
                     /locus_tag="IWM52_003026"
     CDS             <1..1348
                     /locus_tag="IWM52_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HAP9975193.1"
                     /translation="DFILYLKLCNRTESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQI
                     YKMYDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAV
                     ILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKY
                     LIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNT
                     NLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLR
                     QMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFE
                     GKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGE
                     DILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          622 a          117 c          246 g          482 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaaccgaa tcattaaaaa aacattatac
       61 agagccttgg gaagatgaag tttttagagt aattattgaa tataacaatg ttcctattgg
      121 atatggacaa atatataaaa tgtatgatga gttatatact gattatcatt atccaaaaac
      181 tgatgagata gtctatggta tggatcaatt tataggagag ccaaattatt ggagtaaagg
      241 aattggtaca agatatatta aattgatttt tgaatttttg aaaaaagaaa gaaatgctaa
      301 tgcagttatt ttagaccctc ataaaaataa tccaagagca ataagggcat accaaaaatc
      361 tggttttaga attattgaag atttgccaga acatgaatta cacgagggca aaaaagaaga
      421 ttgttattta atggaatata gatatgatga taatgccaca aatgttaagg caatgaaata
      481 tttaattgag cattactttg ataatttcaa agtagatagt attgaaataa tcggtagtgg
      541 ttatgatagt gtggcatatt tagttaataa tgaatacatt tttaaaacaa aatttagtac
      601 taataagaaa aaaggttatg caaaagaaaa agcaatatat aattttttaa atacaaattt
      661 agaaactaat gtaaaaattc ctaatattga atattcgtat attagtgatg aattatctat
      721 actaggttat aaagaaatta aaggaacttt tttaacacca gaaatttatt ctactatgtc
      781 agaagaagaa caaaatttgt taaaacgaga tattgccagt tttttaagac aaatgcacgg
      841 tttagattat acagatatta gtgaatgtac tattgataat aaacaaaatg tattagaaga
      901 gtatatattg ttgcgtgaaa ctatttataa tgatttaact gatatagaaa aagattatat
      961 agaaagtttt atggaaagac taaatgcaac aacagttttt gagggtaaaa agtgtttatg
     1021 ccataatgat tttagttgta atcatctatt gttagatggc aataatagat taactggaat
     1081 aattgatttt ggagattctg gaattataga tgaatattgt gattttatat acttacttga
     1141 agatagtgaa gaagaaatag gaacaaattt tggagaagat atattaagaa tgtatggaaa
     1201 tatagatatt gagaaagcaa aagaatatca agatatagtt gaagaatatt atcctattga
     1261 aactattgtt tatggaatta aaaatattaa acaggaattt atcgaaaatg gtagaaaaga
     1321 aatttataaa aggacttata aagattgatt atataatata tgaaaagcta ttataaaaga
     1381 cattagtatt aaatagttta aaaaaatgaa aaataataaa ggaagtgagt caagtccaga
     1441 ctcctgtgta aaatgctata caatgtt
//
LOCUS       DACDYI010000241         1425 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31910_2017
            SAMN12640115-rid9881223.guided.180, whole genome shotgun sequence.
ACCESSION   DACDYI010000241 DACDYI010000000
VERSION     DACDYI010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640115
            Sequence Read Archive: SRR10023365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741890.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741890.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:32:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,906
            CDSs (with protein)               :: 2,906
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 28 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 10 of 59
            Pseudo Genes (multiple problems)  :: 10 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640115-rid9881223.guided.180"
                     /isolate="E31910_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            315..398
                     /locus_tag="IWM52_003027"
     CDS             315..398
                     /locus_tag="IWM52_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAP9975194.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            523..1260
                     /gene="erm(B)"
                     /locus_tag="IWM52_003028"
     CDS             523..1260
                     /gene="erm(B)"
                     /locus_tag="IWM52_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP9975195.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          528 a          215 c          237 g          445 t
ORIGIN      
        1 taagaaccgt caggtttagc taagtggtga gtagttcact tataaaaaag tggtgcattt
       61 ttaaattggc acaaacaggt aacggttatt gcaggtgtat ttcttatcta tgggtttaac
      121 atggatttta tcattaaaat catgagtatt gtccgagagt gattggtctt gcgtatggtt
      181 aaccctaaag ttatggaaat aagacttaga agcaaactta agagtgtgtt gatagtgcat
      241 taaaaatttt gtataatagg aattgaagtt aaattagatg ctaaaaattt gtaattaaga
      301 aggagggatt cgtcatgttg gtattccaaa tgcgttatgt agataaaaca tctactgttt
      361 tgaaacagac taaaaacagt gattacgcag ataaataaat acgttagatt aattcctacc
      421 agtgactaat cttatgactt tttaaacaga taactaaaat tacaaacaaa tcgtttaact
      481 tctgtatttg tttatagatg taatcacttc aggagtgatt acatgaacaa aaatataaaa
      541 tattctcaaa actttttaac gagtgaaaaa gtactcaacc aaataataaa acaattgaat
      601 ttaaaagaaa ccgataccgt ttacgaaatt ggaacaggta aagggcattt aacgacgaaa
      661 ctggctaaaa taagtaaaca ggtaacgtct attgaattag acagtcatct attcaactta
      721 tcgtcagaaa aattaaaact gaatactcgt gtcactttaa ttcaccaaga tattctacag
      781 tttcaattcc ctaacaaaca gaggtataaa attgttggga gtattcctta ccatttaagc
      841 acacaaatta ttaaaaaagt ggtttttgaa agccatgcgt ctgacatcta tctgattgtt
      901 gaagaaggat tctacaagcg taccttggat attcaccgaa cactagggtt gctcttgcac
      961 actcaagtct cgattcagca attgcttaag ctgccagcgg aatgctttca tcctaaacca
     1021 aaagtaaaca gtgtcttaat aaaacttacc cgccatacca cagatgttcc agataaatat
     1081 tggaagctat atacgtactt tgtttcaaaa tgggtcaatc gagaatatcg tcaactgttt
     1141 actaaaaatc agtttcatca agcaatgaaa cacgccaaag taaacaattt aagtaccgtt
     1201 acttatgagc aagtattgtc tatttttaat agttatctat tatttaacgg gaggaaataa
     1261 ttctatgagt cgcttttgta aatttggaaa gttacacgtt actaaaggga atgtagataa
     1321 attattaggt atagggcacc tctaataact accaattaat tcacctcaaa atgaaattag
     1381 aaaaaaacag cgaaattttt aaatctattt cttatcgata caaat
//
